LOCUS       DANLRK010000001        42039 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.001, whole genome shotgun sequence.
ACCESSION   DANLRK010000001 DANLRK010000000
VERSION     DANLRK010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 42039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42039
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.001"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            147..1505
                     /locus_tag="PXA33_000001"
     CDS             147..1505
                     /locus_tag="PXA33_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HDL5119391.1"
                     /translation="MITIKKGLDLPIAGKPEQVIHNGNTVTEVAMLGEEYVGMRPSMK
                     VREGDVVKKGQVLFEDKKNPGVVFTAPASGTVTAINRGEKRVLQSVVIKVEGDEQITF
                     ARYEASQLASLTSEQVKQNLVESGLWTAFRTRPFSKVPALDAEPSSIFVNAMDTNPLA
                     ANPEVIIAEHQQAFFDGLTVLSRLFESKKVIHLCGSADSNILNAAQSRQINNVETNRF
                     KGPHPAGLSGTHIHFIDPVGATKQVWYLNYQDVIAIGKLFTTGELFTDRVVALAGPQV
                     KNPRLVRTRLGANLSQLTANELNAGENRVISGSVLSGAIATGVHDYLGRYALQVSALL
                     EGREKAFFGMIAPYSDKYSITRTTLGHFAKKLFNFTTAVHGSERAMVPTGAYERVMPL
                     DIIPTLLLRDLAAGDTDSAQALGCLELDEEDLALCTFVCSGKNNWGPLLRQALDKIEK
                     DG"
     gene            1508..2743
                     /locus_tag="PXA33_000002"
     CDS             1508..2743
                     /locus_tag="PXA33_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HDL5119392.1"
                     /translation="MGLKNLFEKMEPAFHKGGKYEKWYTLFEAAYTIFYTPGTVTRKD
                     AHVRDAIDSKRMMLIVWLALFPAMFYGMYNVGAQGILAALKMGTLTDLVANNWHYSLA
                     DSLGSVADAGWGTKMLLGATYFLPIYLTVFAVGGFWEVVFAMVRKHEINEGFFVTSIL
                     LALIVPPTLPLWQAALATTFGVVVAKEVFGGVGKNFMNPALAGRAFLFFAYPGQISGD
                     AVWVAADGFSGATALSQWAVGGQGALQHVATGETITWMDAFLGNIPGSIGETSTLMLI
                     IGAAIIVFARIASWRIIAGVMIGMAATATLFNVIGSDTNPLFSMPWHWHLVLGGFAIG
                     MFFMATDPVSAAFTNKGKWAYGILIGFMCVLIRVVNPAYPEGMMLAILFANLFAPIFD
                     YLVVQGNIKRRLARVANNG"
     gene            2736..3509
                     /locus_tag="PXA33_000003"
     CDS             2736..3509
                     /locus_tag="PXA33_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit C"
                     /protein_id="HDL5119393.1"
                     /translation="MAKFNKDSVGGTLTVVVLLSLICSLIVAGAAVMLKPTQDIQKQL
                     DKQKNILQAAGLLQKDTNVQETYTKFIEPKIVDLATGDYVEGVTGFDARAAAKDPAQN
                     VVIAPEDDKANIKTRAKYAEVYLVKDESGKTTQVVLPMHGNGLWSIMYGFVAVQPDAN
                     TVNGITYYEQGETAGLGGEIANPNWQKNFVGKKLYNEQNQVALRVGKGASADKDHGVD
                     GLSGATLTSNGVDGSFKYWFGQNGFGPYLAKFKAAAGAN"
     gene            3509..4135
                     /locus_tag="PXA33_000004"
     CDS             3509..4135
                     /locus_tag="PXA33_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005717817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HDL5119394.1"
                     /translation="MADNNNLKKLLLSPIVDNNPIALQILGICSALAVTTQLQTAVVM
                     AIAVSLVTAFSSMFISMIRNYIPNSIRIIVQMAIIASLVILVDQILRAYAYDLSKQLS
                     VFVGLIITNCIVMGRAEAFAMKSGPVESFVDGIGNGLGYGAMLIIVAFLRELIGSGKI
                     FGITILETVQNGGWYQANGLFLLAPSAFFIIGFVIWAIRTWKPEQVEK"
     gene            4137..4733
                     /gene="nqrE"
                     /locus_tag="PXA33_000005"
     CDS             4137..4733
                     /gene="nqrE"
                     /locus_tag="PXA33_000005"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005529392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HDL5119395.1"
                     /translation="MEHLLSIFVKSIFIENMALSFFLGMCTFLAVSKKVSTAFGLGIA
                     VIVVLGIAVPANQLVYTHVLKEGALVEGVDLSFLNFITFIGVIAALVQILEMILDKFF
                     PALYSALGIFLPLITVNCAIFGGVSFMVQREYNFVESVVYGVGAGTGWMLAIVALAGL
                     TEKMKYSDVPAGLRGLGITFITVGLMALGFMSFSGIQL"
     gene            4744..5973
                     /gene="nqrF"
                     /locus_tag="PXA33_000006"
     CDS             4744..5973
                     /gene="nqrF"
                     /locus_tag="PXA33_000006"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HDL5119396.1"
                     /translation="MDSNFIFGIIAFTALVLVLAVIILFAKSKLVDSGDITISINNDP
                     EKGITLPAGGKLLGALASKGIFVSSACGGGGSCGQCKVQVKSGGGEILPTELSHISKK
                     EAKEGWRLACQVNVKSSMDVELPEEIFGVKKWECTVISNDNKATFIKELKLQIPEGEE
                     VPFRAGGYIQIEAEPHTVHYKDFDIPKEYHEDWDKFDLWRYTSKVDEHIIRAYSMASY
                     PEEKGIIMLNVRIATPPPRNPDVPPGQMSSYIWSLKPGDKVTISGPFGEFFAKDTDAE
                     MVFIGGGAGMAPMRSHIFDQLKRLKSKRKMSFWYGARSEREMFYVEDFDGLQAENDNF
                     KWYVALSDPLPGDREDYFRGFIHNVLYENYLKNHEAPEDCEYYMCGPPVMNAAVIGML
                     KSLGVEDENILLDDFGG"
     gene            6085..6519
                     /locus_tag="PXA33_000007"
     CDS             6085..6519
                     /locus_tag="PXA33_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase substrate binding
                     protein"
                     /protein_id="HDL5119397.1"
                     /translation="MEQLDITVLENAFNSLEKTIVSLENQAWFNRQEAIIQDTLIAGA
                     IQKFEFVYELSIKMLKRQLKNIESNDTDIDSTEFRDILRMAAQYGLIESPEVWLDYRK
                     MRNITSHTYDQNKAQQVYNGISNFLESTYFLVKQLQKRNSYD"
     gene            6512..6805
                     /locus_tag="PXA33_000008"
     CDS             6512..6805
                     /locus_tag="PXA33_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HDL5119398.1"
                     /translation="MIDLTESQRQQVLDIFKKYLPDTPVWVFGSRIKGTAKAYSDLDL
                     ALITKQPLTIRQQTELELAFTDSDLPFTVDLIDWASCNESFKQIILQRYEVLI"
     gene            6854..7888
                     /locus_tag="PXA33_000009"
     CDS             6854..7888
                     /locus_tag="PXA33_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HDL5119399.1"
                     /translation="MKKFFLLLIAFFTLSACEKAPEQITLSGKTMGTTYTVKYIDNGE
                     VENLISPEKMKNELDGLLIEVNNQMSTYQQDSEISRFNQAKQANQAVEISQDFAKVVQ
                     EAIRLNKVTEGALDVTVGPLVNLWGFGPDKRLNKVPSDEQIAERSAYVGIDKILLEMS
                     SKPKLTKSAANLYLDLSSIAKGFGVDKLAEHLETIGVSNYLVEIGGELRGKGKNLQGL
                     DWRIAIEKPVMTQGQAAQITVPLHNLAMATSGNYRNYFEDEKGNRLSHIIDPKQLRPV
                     NHNLASITVFAPTTMTADGLSTGLFVLGAEKALAVAEREKLAIFLIVKNGDKFETQMS
                     SEFSKLIQSQ"
     gene            7901..8146
                     /gene="nqrM"
                     /locus_tag="PXA33_000010"
     CDS             7901..8146
                     /gene="nqrM"
                     /locus_tag="PXA33_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Na+)-NQR maturation NqrM"
                     /protein_id="HDL5119400.1"
                     /translation="METVLLTFGFFVAVIFAMSIGFIVKGKTIKGSCGGITALGMKKM
                     CDCEEPCDNLKSKIADGTADPEEVARFNKEPQFYEVK"
     gene            8148..8564
                     /locus_tag="PXA33_000011"
     CDS             8148..8564
                     /locus_tag="PXA33_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase substrate binding
                     protein"
                     /protein_id="HDL5119401.1"
                     /translation="MNRDIRWKQRFDNYKKSVSYLQAEAEKYADTDIDVIKKGIIQSF
                     EITHELAWKLMQDILKWEGEVDIYGSKTASRLAFNRGLITQGEVWLEMIKSRNITVHT
                     DDETILTEEFNKIIKAYLPLFIEFEQRVETLCQTLD"
     gene            8546..8857
                     /locus_tag="PXA33_000012"
     CDS             8546..8857
                     /locus_tag="PXA33_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HDL5119402.1"
                     /translation="MPNFGLSERATKTITDILAKYPEVEQAIIYGSRAKGNYREGSDV
                     DLTFKGKDLTYDIVKRIWGDLDDSDSPYLFDLSIYHQLTNANFIEHIDRVGKVFYQRE
                     L"
     gene            8968..10131
                     /gene="mnmA"
                     /locus_tag="PXA33_000013"
     CDS             8968..10131
                     /gene="mnmA"
                     /locus_tag="PXA33_000013"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HDL5119403.1"
                     /translation="MTTQLTSKTYDTHFPKLTPEQLAENSKKKVIIGMSGGVDSSVSA
                     FILQQQGYQVEGLFMKNWEEDDDTDYCTAAAELADAQAVADKLGMKLHKINFAAEYWD
                     NVFEYFLAEYKAGRTPNPDILCNKEIKFKAFLEYAAEDLGADYIATGHYVRRTSDDQD
                     AKLLRGLDENKDQSYFLYALNKKQIGQSLFPVGEIEKPIVRAIAEDLGLATAKKKDST
                     GICFIGERKFKDFLARFLPAQPGEIRTVEGKLVGRHDGLMYHTLGQRKGLGIGGVKGL
                     SEDPFYVVEKDLINNVLIVAQGHDNSALLSSGLIAKQLTWVDMQPLRENLRCTVKTRY
                     RQADIACEIQVIDDETIRVMFDEPQIAVTPGQSAVFYLGEVCLGGGIIESQLK"
     gene            10248..11627
                     /gene="argH"
                     /locus_tag="PXA33_000014"
     CDS             10248..11627
                     /gene="argH"
                     /locus_tag="PXA33_000014"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HDL5119404.1"
                     /translation="MALWGGRFTQQADAKFKYFNDSLRFDYRLAIQDIEGSIGWAKAI
                     TSVGILTVAELEQLIAALKEVRAEVESNLTIILKDDAEDIHSWVESKLIEKVGDLGKK
                     LHTGRSRNDQVAVDIKMWCKVQAVALQERIRALQERLVSVAEENQESVMPGYTHLQRA
                     QPVTFAHWCMAYYEMLERDFSRLSDANKRMSTCPLGSGALAGTAYGIDRDLLARDLGF
                     EEATRNSLDSVSDRDHILELLSTASISMMHLSRFAEDLIIFNSGESGFLEMSDRVTSG
                     SSLMPQKKNPDACELIRGKSGRVFGALNGLLTTLKGLPLAYNKDMQEDKEGIFDALET
                     WQACLEIAELVLVDIQVNTERTREAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VYAISKKEPLEALSINEFQQFHHSIGDDVYPILSLESCLEKRCAKGGVNPQRVAEAIA
                     VAKANLASK"
     gene            11795..12793
                     /locus_tag="PXA33_000015"
     CDS             11795..12793
                     /locus_tag="PXA33_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HDL5119405.1"
                     /translation="MTESVNSTQAGTALSLGQQLKAAREALNLSITDVAQKTNLKKSH
                     IESLENDIFILQNVAPTFVRGYVRNYVKFLRLPESLISSVNYGEVTIPKEVTKVSSVK
                     PSSNSQGKTLKYLTVLVLLAALGMTLLWWWQGYQKEQENREQFVSSAPVAEVANTATN
                     NDASAQTSVPVQQPIQVSEQPAQVAETVVAAEPALTEKVEPTQAASSPVTQEDVKVVE
                     EVKQADATTAQVEAVNVLQQSQSEQAPTEQPVAVTNDELRIEITGSQSWITVRGAKNK
                     RLAEKLYNNGETLSFNENEQYRLTIGAPANVKVYYKGQEVPLKIDGRVARIHLPLQ"
     gene            12894..14006
                     /gene="ispG"
                     /locus_tag="PXA33_000016"
                     /gene_synonym="gcpE"
     CDS             12894..14006
                     /gene="ispG"
                     /locus_tag="PXA33_000016"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HDL5119406.1"
                     /translation="MIHQSPIKRRVSKKIYVGNVPVGGDAPITVQSMTNTRTTDVEAT
                     VAQIKSLERVGADIVRVSVPTMDAAEAFKAIKQQVSVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRTVVDCARDKNIPIRIGVNAGSLERDLQEKYGEPTPQALLES
                     ALRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARAGSV
                     KSAVGLGLLLAEGIGDTLRVSLAADPVEEVKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EIDVIATVNELEQRLEDIITPMDVSIIGCVVNGPGEALVSDLGVTGSNKMSGFYLDGV
                     RQKERFDNAKLIDQLEAKIRARVAQQHNRIDIEQIG"
     gene            14012..15283
                     /gene="hisS"
                     /locus_tag="PXA33_000017"
     CDS             14012..15283
                     /gene="hisS"
                     /locus_tag="PXA33_000017"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HDL5119407.1"
                     /translation="MAKTIQAIRGMNDCSPSESPLWQWVEQKVRNVLASYGYSEVRMP
                     IVESTPLFARAIGEVTDVVSKEMYTFWDNDEQLTLRPEGTAGCVRAAIERGWIYNNEQ
                     RLWYMGPMFRHERPQKGRYRQFHQAGVEVFGIANPEIDAELIILTARLWKELGIDQHV
                     SLQLNSIGSLEARANYRSALVKFLENHTALMNEEEKERLVKNPLRILDTKNEALQNVL
                     NDAPKLLDYLDDDSREHFTQLCALLDAMGIRYEVNPKLVRGLDYYNKTVFEWVTSALG
                     SQGTVCGGGRYDGLVEQLGGHATQGVGFAMGLERLVLLVQEVNKEIDLPKTVDVYIVF
                     SGEGATLNAFQLAEQIRSELPHVGVMTHCSGGKFQKQFKRADKVEAQIALVIGESEVA
                     EKQVVIKDLRSGAEQITIAQSELIAELSKRF"
     gene            15293..15913
                     /locus_tag="PXA33_000018"
     CDS             15293..15913
                     /locus_tag="PXA33_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDL5119408.1"
                     /translation="MSNEYLNKTEEQQFEDAKNWFKENGTPILVAIILACAATAGWNF
                     WKNHQLETAQKTSASYQSVMESYLQNPSKNQPLVEKFIADNKDSSYAAFAQFEVAKQA
                     VEKGNFSAAKTALQSVLTYSDSTLQSIARFRLAQIDFQLKSYDEALASLAQIQDKAWE
                     LRKQILTGDILAAKGDIPAAKSAYEQAKTTANPQEQTLIDVRLNNL"
     gene            complement(15976..17079)
                     /gene="dnaN"
                     /locus_tag="PXA33_000019"
     CDS             complement(15976..17079)
                     /gene="dnaN"
                     /locus_tag="PXA33_000019"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HDL5119409.1"
                     /translation="MQITITREQLLKPLQQVCGVLSSRPTLPVINNLLLEIEGNRLSL
                     TGTDLEVELTTQAQLLQAVDFAGKFTIPAKKFLDISKSLPDDSVISVQFEEDKAIIKA
                     GRSKFSLATLPASDYPNLMDWKPEVDFSIDQSTLARLIDATQFSMANQDARYFLNGMK
                     FETEGNLLRTVATDGHRLAVCTMVLNQELLTHSVIVPRKAVLELSRLVASNDETVRLE
                     IGSSNIRITMNGIVFTSKLIDGRFPDYRRVLPRNADRILEAETETLKRALMRAAILSN
                     EKFRGVRLTLSNNLLKITANNPEQEEAEEIIDVAYDSPEMEVGFNVSYVLDVLNTLKC
                     QRVRFSLVDSSSSCLIEDCDNSSAEYVIMPMRL"
     gene            complement(17098..18447)
                     /gene="dnaA"
                     /locus_tag="PXA33_000020"
     CDS             complement(17098..18447)
                     /gene="dnaA"
                     /locus_tag="PXA33_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005609583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HDL5119410.1"
                     /translation="MEQIVSSLWSDCLNHLQTKVSPTDYSTWLRPLQASSENGELTLY
                     AQNQFVENWVKDKFLSEITELARFLAKNDNLVVTLKVGIKPTEQPKVSPNLKNESSEF
                     SNTGFKTGLNPNQTFDNFVQGRSNQLAKLVAQQVVDNLGESHCNPLSFYAGTGLGKTH
                     LLHAIGNELVKRTPGTRVLYIHLERFYRDIIKAINSSNNDAEKIKKFYRSLDVLMIDD
                     IQFLANKPKVQEEFLHLLNALFEQRKQIILTSDVLPKNIDKIDTAIKSRLSGGISATI
                     EPPELETRVAILRQKALERGADLQEDAAYLMGQKLRTNVRELEGALNRVIAWQKLTLR
                     PITVDAVRETLQDLFASFDYLITIENIQRIVAEYYGIKVADIKSKSRARSIARPRQMA
                     MALAKELTNHSLPEIGREFGGRDHTTVMHACKTINELRDTESGIQEDYIHLTRKLSS"
     gene            complement(18634..20481)
                     /locus_tag="PXA33_000021"
     CDS             complement(18634..20481)
                     /locus_tag="PXA33_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL5119411.1"
                     /translation="MFEKLFKWFEARVETYPNEMPKTPKSGLIPFIYDATKGMRFYIL
                     LLTVLVAAVGIIEAVLFRFMGDLVDWVNKYSPTELWAEKGWTIIGMFFIALLAVVFVY
                     LASSIRFQSLQGVFPMRLRWNFHRLMLGQSLGFYQDEFAGRVSAKVMQTALSVRDVIM
                     TCADMLVYVLVYFTTSSVILLQLDGWLFVPFILWVISLAATIIYFVPKLAQAAQEQSD
                     ARSLMTGRITDAYANIATVKLFSHGNRESAYAKESMEGFMVTVHKQMRLVTVIETVTN
                     LTSMALIISTAGIGLWLWGSSVVTAGAIATSTALALRIKGLSQWIMWEFARLFENLGT
                     VQDGMITLSKPHNVVDKIDAKPLNVTKGEIKFENVDFAYDPKKPLLKDFDLTIKPGEK
                     VGLVGRSGAGKSTLTNLLLRFYDIQDGSITIDGQNIRDITQESLRSQIGLVTQDTSLL
                     HRSVRENLMYGRPNATEEEMIKAVEQAAASEFIPNLQDAKGRTGFDAHVGERGVKLSG
                     GQRQRIAIARVMLKDAPILLLDEATSALDSEVEVAIQENLTQLMEGKTVVAIAHRLST
                     IMAMDRLIVLDKGEIVEQGTHEELLAQNGVYARLWAHQSGGFLPENTDI"
     gene            complement(20550..21059)
                     /gene="luxS"
                     /locus_tag="PXA33_000022"
     CDS             complement(20550..21059)
                     /gene="luxS"
                     /locus_tag="PXA33_000022"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005706864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HDL5119412.1"
                     /translation="MPLLDSFKVDHTKMNAPAVRVAKTMTTPKGDTITVFDLRFCRPN
                     IDILPVRGIHTMEHLFAGFMRDHLNSEQVEIIDISPMGCRTGFYMSLIGSPSAEEVVK
                     AWTASMEDALHKIPDVSAIPELNEYQCGSYKEHSLKEAHQIARNVLAAGIGINRNEDL
                     ALDEKLLNP"
     gene            complement(21077..23743)
                     /locus_tag="PXA33_000023"
     CDS             complement(21077..23743)
                     /locus_tag="PXA33_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HDL5119413.1"
                     /translation="MTMEENTHTVVDAQIRQPRKISPFWLLPIVAFIIGALLFFQILK
                     EQGETITIRFSKGDGITAGKTAIRYQGLQIGQVKRVYFVDNLKEVEVQAEINPEAKSI
                     LRKQTKFWLVQPSASLAGVSGLDAIVSGNYITLLPGEGDFVDEFIAEEDPPAITVSDG
                     DLLIRLISDDIGSITVGASVYFRKVPVGNIADYRFTKDQKKIEIDVVINQKYAHLVKK
                     ESRFWNISGISADVNLQGIKINMDSLASVVQGAVAFDSPDNMEIAEQGQKFELYPDLK
                     SAKRGIEINVKLPLTHNLKINETGVFHQNVQIGMLSQLKLPQVKDNGVPQQESGQHKQ
                     IDGVLLIDPSYENLLRSQTKILLKEPKFSLNTEQLTKVGELLRGVYFEVHPGEGEPQS
                     EFVVQNEADYLLSLPNLLSFSLIAPQAYGVDTNQGIYYSDVQIGEIVKRSLELDKVRF
                     DVIIYPPYRNLIGSNSKFVAISNIDMSVGLDGLRVHAGSPSEWLKGGVRLINGKNDGS
                     PKKQYALYKDIESAESGMATLDKKATTTLSADSLSGISEGSLVLYRDFQVGEVLKITP
                     KQQKFEVELFIQPNYRHLLTEKSRFWIEPATSVDVSLKGVNITTAPLMRTLKGAISFD
                     DGGSKGNKTLYASKEKATSGNTYLTLIAKDASKLSEGMDIKYMGLTVGQIEKLELQNA
                     KKQIKATAYIQRKYYPLIAKAGSKFNVISPEISTSGFKNLDAALQNYITVDVGSGQAQ
                     NQFVLADTDTVKTTYANGFPIIVETTNANGIQPEAPVLYRGMQVGIVSRLGLSELGDR
                     VLIYVNIQDKYKHLVRTNTQFWQASGYTMDVSLQGVSMNSGTMSQLLNGGIEFSTPYT
                     KVVKPQAQPNRRFFLQRKLPDEAPAWDQGIAE"
     gene            complement(23826..24266)
                     /gene="fur"
                     /locus_tag="PXA33_000024"
     CDS             complement(23826..24266)
                     /gene="fur"
                     /locus_tag="PXA33_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014990964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HDL5119414.1"
                     /translation="MSEANIKRLKEVGLKVTEPRLTILALMQDIRDEMQHFAAEDIYK
                     ILLEKGSDIGLATVYRVLNQFEEAGILTRHNFDANKAVFELNMNHEHDHIICMDCGKV
                     FEFKDPDMERRQREISEKHGMELTNHSLYLYGKCSNLTNCDEKK"
     gene            complement(24302..24826)
                     /gene="fldA"
                     /locus_tag="PXA33_000025"
     CDS             complement(24302..24826)
                     /gene="fldA"
                     /locus_tag="PXA33_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006717621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HDL5119415.1"
                     /translation="MAVVGLFYGSDTGNTENISKMIQKKLGAELIDIRDIAKSTKEDI
                     EAYDFLMFGIPTWYYGESQADWDDFMPTLKEIDFNGKVVAIFGCGDQEDYAEYFCDAM
                     GTVRDVVEPNGGVIVGHWPTAGYTFEVSQALVDDDTFVGLCIDEDRQPELTTERVNKW
                     CEQLKEEMCLDQLA"
     gene            complement(24832..25107)
                     /gene="ybfE"
                     /locus_tag="PXA33_000026"
     CDS             complement(24832..25107)
                     /gene="ybfE"
                     /locus_tag="PXA33_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HDL5119416.1"
                     /translation="MAKQDSDCITLDLFATVPKVGRPRTNPLDREQQLRINKRNQLKR
                     DKSSGLKRVELKLHSNLVKLLEEQASEQGVSRGQLIETILNNYLTNR"
     gene            25348..26334
                     /locus_tag="PXA33_000027"
     CDS             25348..26334
                     /locus_tag="PXA33_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDL5119417.1"
                     /translation="MSNIHLAIAADFTLAEKLLEALANQDLGIENISAVELEPFGEEQ
                     NLRFGAKAVAQYALDEVNWAEFSHVLFAGKMAQAEVLAEAVKAGCVILDLYGITALIG
                     DVPVVVPSVNDENVANLRERNIVALANPQISQLALALKPLLNEPLKQVWVTSLLPSAY
                     FGDEKVKELAGQTARLLNGIPFDENAERIAFDVVPANVQGEEKSLPFSKAFELQIAKV
                     LPNLTACTTFHSVQVPVFYGVSQMVSVQSEYQLDANALSEAWKAENWLNFNENSVITA
                     VKNGENEEQNALEISQLLAKSEQEVQFWTVADEQRFSLAFLAVKLLKLVLAY"
     gene            26431..27726
                     /locus_tag="PXA33_000028"
     CDS             26431..27726
                     /locus_tag="PXA33_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDL5119418.1"
                     /translation="MLEKLFQLKAKGSNVKTEIIAGITTFFTMVYIVFVNPSILGVAG
                     MDTQVVFVTTCLIAAFGTIAMGLFSNLPIALAPAMGLNAFFAFVVVQKLGYSWQVGMG
                     AIFLGSVGLFLLTVLQVRYWFMSAIPLGLRVGIGAGIGLFIALIGFKNMGLVVPNPAT
                     LVALGDLHDPKVLMGILGFFIIVVLAAKGIHSGVLISIVIVTALALAFDPAVSFNGIM
                     SMPPSLGAVVGQVDIAGAFDVALLGMIFSFMLVNLFDSSGTLIAVTTKAGFADEQGRF
                     PKMKQALLVDSTAAMAGSYMGTSAISTYIESGSGVSVGGRTGLTAVVVGILFLLTIFF
                     SPLAGLVPAYATAGALVFVGILMASSLIEVKWDDLTEATPAFITTAMMPFTYSITEGI
                     AFGFISYCVMKAFTGRWKEINLSVLVVSLLFIAKFVWVG"
     gene            27846..28214
                     /locus_tag="PXA33_000029"
     CDS             27846..28214
                     /locus_tag="PXA33_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/TusD sulfur relay family protein"
                     /protein_id="HDL5119419.1"
                     /translation="MSNSVQKLLFIINSAPYGNETFFSALRLALQIQEQHKANTEIKL
                     FLMSDAVTAGLAKQNPAEGYHIQQMLEILTAQGANVKLCKTCTNARGITDLPLADGVE
                     IGTLIELADWTIQADKVLNF"
     gene            28214..29407
                     /locus_tag="PXA33_000030"
     CDS             28214..29407
                     /locus_tag="PXA33_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL5119420.1"
                     /translation="MQNIDVVVIGAGAAGLFCAAQLGQAGKKVVVLDSGKKVGRKILM
                     SGGGFCNFTNLDVSAGNYLSQNRHFVKSALSRYTQWDFISLVSHYGISYHEKELGQLF
                     CDESSQQIVDLLQAECNKGKVEILLRQAVISVEKVANFFEISTACGKFQSEHLVIATG
                     GLSMPALGASPFGYQLAEQFGIPVVPPRASLVPFTWKESDKYFAELSGIALPITVSAN
                     GKSFSNQMLFTHRGLSGPAILQISNYWELGETVEIDLLPSQSITDILSELRQSSPKLQ
                     LKTVLSRYLPKKLVEVWLEQKLLTDKVIADLSKKDLTLLEQFIHHWHFLPNGTEGYRT
                     AEVTKGGVDTDYISSKTMEAKNTAGLYFIGEVLDVTGWLGGYNFQWAWSSAFACAEGI
                     IEKSK"
     gene            complement(29478..29696)
                     /gene="infA"
                     /locus_tag="PXA33_000031"
     CDS             complement(29478..29696)
                     /gene="infA"
                     /locus_tag="PXA33_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HDL5119421.1"
                     /translation="MAKEDCIEMQGTILETLPNTMFRVELENGHIVTAHISGKMRKNY
                     IRILTGDKVTVEMTPYDLSKARIIFRAR"
     gene            complement(29806..30465)
                     /locus_tag="PXA33_000032"
     CDS             complement(29806..30465)
                     /locus_tag="PXA33_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HDL5119422.1"
                     /translation="MYISREKITKTLLLAGITLLCCGIVAVILMGVNQFKLGSQLASI
                     NQVSNLSHLLVRQQANIFSFLLVKNAKTDELVDALDSFAKEDFIIDANIYSPNGVLLA
                     QSQNAKDLKVLLDSEQSAKTQQIVEPIFSQQALVGFLRVTFDAEYGQTTKARVEQQFH
                     QLYGEIIILLLTGILLASSINYYFRRKIHHVQISPKPLMSQVKTQTQRFHSRRRWIKR
                     K"
     gene            30551..31414
                     /gene="serB"
                     /locus_tag="PXA33_000033"
     CDS             30551..31414
                     /gene="serB"
                     /locus_tag="PXA33_000033"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HDL5119423.1"
                     /translation="MTNTIFIYSKTLNEREIEQFSQQTAAILLKQAVYLDYKIAFFST
                     NLTACELRETAQKINADISDLTIIPTLNQGGLLLMDMDSTAIKIECIDDIAKLAGTGE
                     VVSAITASAMRGELDFEQSLRKRVGTLENASESILQKVRENLPLMDGFEQMVAILKEH
                     GWKLAIASGGFDYFADYLKQKYGLNFAVSNQLEIIDGKLTGKVLGKVVDAQHKADTLQ
                     NLATTFNIPPNQWVAVGDGANDLPMLKTASLGVALHAKPKVQAQADFVVNFGDLTALC
                     LLLNANHLFQH"
     gene            31430..31921
                     /locus_tag="PXA33_000034"
     CDS             31430..31921
                     /locus_tag="PXA33_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HDL5119424.1"
                     /translation="MPSFDIVSEITMHEVRNAVENANRVLSTRYDFRGVEAVIELNEK
                     NESIKLTTESDFQLEQLIEILIGSCVKRGIEHSSLDIPTESEHHGKLYSKEIKLKQGI
                     ESDMAKKITKLIKDSKIKVQAQIQGEQVRVTGKSRDDLQAAIQLVKGADLGQPFQFNN
                     FRD"
     gene            32006..32182
                     /locus_tag="PXA33_000035"
     CDS             32006..32182
                     /locus_tag="PXA33_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5363 domain-containing protein"
                     /protein_id="HDL5119425.1"
                     /translation="MMSEQTEKKGWFKKALEKYDAFCDELGVNQGACRGCVPVVKFDP
                     EPEKEQPEKSENKT"
     gene            complement(32230..32526)
                     /locus_tag="PXA33_000036"
     CDS             complement(32230..32526)
                     /locus_tag="PXA33_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5119426.1"
                     /translation="MKKHHIIILTLASASVVACSSNTPVQVVNGDKNIDYSQPKTIYI
                     SETNTQPPALTKQEKIKPSNATALCFNGSYSTDIQNPCKKDGGVKQYFAHHHAN"
     gene            complement(32603..33571)
                     /gene="mdh"
                     /locus_tag="PXA33_000037"
     CDS             complement(32603..33571)
                     /gene="mdh"
                     /locus_tag="PXA33_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HDL5119427.1"
                     /translation="MCQSKKVALLGAAGGIGQSLALLLKLNLPAKSELSLYDISPVTP
                     GIAVDLSHIPTDVKVTGFAGEDPTEALKDADVVVISAGVARKPGMTRADLFNTNATIV
                     HNLVEKAAKVCPKACIAIITNPVNTIIPIAAEVLKKAGVYDKNKLFGVTTLDVIRANT
                     FVAEAKDVNVKYVRVPVIGGHSGTTILPLLSQATVNGLKLEFTQEQIEQLTHRIQNAG
                     TEVVEAKAGGGSATLSMAQAGAEFALGLVRALIGEDVIRYAYVDNANGETSPAFFAYP
                     IRLGTNGVEKVLPIGNLSEFEKDQLEQLIPVLNDEIQLGQTFNKNA"
     gene            33767..34228
                     /gene="argR"
                     /locus_tag="PXA33_000038"
     CDS             33767..34228
                     /gene="argR"
                     /locus_tag="PXA33_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005713376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HDL5119428.1"
                     /translation="MDKIDSLSEAFKSLLKEEKFGSQSEIVAALQEMGFDNINQSKVS
                     RMLSKFGAVRTRNPKMEMVYQLPVELSVPTTSSPLKNLVVDIDHNDALIVVKTSPGAA
                     QLIARLLDSMGKAEGILGTIAGDDTIFITPVRNVQIEKLVKNVTQLFETSL"
     gene            34278..35174
                     /locus_tag="PXA33_000039"
     CDS             34278..35174
                     /locus_tag="PXA33_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HDL5119429.1"
                     /translation="MHIFITGGTGFIGRELIQQLKSENHHFTLLTRDTEAKSDDLQIN
                     YCYSLDELVDLNDFDAVINLAGEPIFDKRWTDEQKQKLIESRVNLTNKLSQLMNASSN
                     PPHTFLSGSAIGIYGDLPPSANFYTESTACYQDCFTNRLCQQWEAAALKAASDKTRVC
                     LIRTGTVFSPEGGALKKILPLYRCGLGGKLGSGKQYWAWISRRDHVQAVIFLLKNANC
                     SGAYNFVAPNPVRNEEFNRILANAVNRPAFFTVPEFALKLGLGERACLVLDNQQLIPE
                     KLLAASFVFQDPILTENVFLNP"
     gene            35219..37417
                     /gene="uvrD"
                     /locus_tag="PXA33_000040"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             35219..37417
                     /gene="uvrD"
                     /locus_tag="PXA33_000040"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HDL5119430.1"
                     /translation="MDFSLLLDGLNDKQREAVAAPIGNYLVLAGAGSGKTRVLTHRIA
                     WLIGVENVPESNILAVTFTNKAAAEMRHRIEYTLSQSGDNRLFGMWVGTFHSIANRLL
                     RSHYLDADLPQDFQIMDTEDQQRLLKRLLKLHNIDEKHFPPKHVAWYINAQKDKGKRA
                     KDIDHYNDPNEKKLVEIYQIYQDACDRAGLLDFAELLIRAYELFKTKSTILQRYQQRF
                     QQILIDEFQDTNNIQYDLIRLLAGDVGKVMIVGDDDQSIYGWRGAQVENIQRFLNDYD
                     NAQTIRLEQNYRSTGNILSCANELIANNDNRLGKNLWTDSGDGEPVDIYCAFNELDEA
                     RFVASQIKQWKEDEGDLAECAVLYRSNSQSRVIEEALIQANIPYRIYGGMRFFERQEI
                     KDALAYLRLIANRQDDAAFERVINTPTRGIGDRTLDTLRQITRGRQITLWQAIQVAVQ
                     EEQLSGRATSSLLRFVELINALDQETEQMSLAEQTDFVIKKSGLYEMYKQEKGEKGEV
                     RIENLEELVTATKQFVKPDEAEELTDLTAFLTHASLEAGEAQASQHEDYVELMTLHSA
                     KGLEFPRVFIVGVEEGIFPSGMSFDEGRLQEERRLAYVGITRAKKKLTISYAESRRLY
                     GKEERHVPSRFIAELPEEHIREVRLRGSINRAASFNQANSFAKNPTKTTASFLNDDTW
                     KMGQKVNHAKFGQGTIINVEGSGEATRLQVAFVGNGIKWLIAKMANLERI"
     gene            complement(37564..37638)
                     /locus_tag="PXA33_000041"
     tRNA            complement(37564..37638)
                     /locus_tag="PXA33_000041"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(37604..37606),aa:Gln,seq:ttg)
     gene            complement(37693..37767)
                     /locus_tag="PXA33_000042"
     tRNA            complement(37693..37767)
                     /locus_tag="PXA33_000042"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(37733..37735),aa:Gln,seq:ttg)
     gene            complement(37793..37877)
                     /locus_tag="PXA33_000043"
     tRNA            complement(37793..37877)
                     /locus_tag="PXA33_000043"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(37841..37843),aa:Leu,seq:tag)
     gene            complement(37880..37956)
                     /locus_tag="PXA33_000044"
     tRNA            complement(37880..37956)
                     /locus_tag="PXA33_000044"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(37920..37922),aa:Met,seq:cat)
     gene            complement(38123..38947)
                     /gene="aroE"
                     /locus_tag="PXA33_000045"
     CDS             complement(38123..38947)
                     /gene="aroE"
                     /locus_tag="PXA33_000045"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005615478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HDL5119431.1"
                     /translation="MNRYAVWGNPIAQSKSPRIHQLFALQTQRNIEYIANLGDETAFE
                     QQLADFFASGAKGCNITAPFKERAFKLADEFSETALLAQACNTLKRLEDGRLYADNTD
                     GAGLVSDLERLGWLQKGHKTLILGAGGATKGVLLPLLKAGLEITLYNRTQEKAVVLAE
                     KFAKFGKIQTACLSDLANQLFNLIVNATSLGLQGKYVELPESVIASANIYDMQYAPNM
                     QTPFLNYARRCGATACQDGLGMLVGQAAFAFSLWEGQFPDVEPVLAQLKLEMQTVK"
     gene            complement(38954..39508)
                     /locus_tag="PXA33_000046"
     CDS             complement(38954..39508)
                     /locus_tag="PXA33_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HDL5119432.1"
                     /translation="MNNFLEIVNQLKQEEVVAYPTEAVFGLGCNPQSEKAVRKLLVLK
                     QRPEEKGLILLASDLKYLLPYIDEARMTEQAWQRLSHIGEQAITWIVPVKNTVPSYLT
                     GKFDSIAVRLCNVPAVVQLCEASGVALTSTSANLSGEPPCRNAKEVIDQFGQDFPVLH
                     ADTLGRENPSEIRDIFSQQIFRQG"
     gene            complement(39501..40037)
                     /locus_tag="PXA33_000047"
     CDS             complement(39501..40037)
                     /locus_tag="PXA33_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HDL5119433.1"
                     /translation="MVLFKSTKQTETCPQCGSPLQIKQGKKGLFLGCSAYPECDYIKP
                     LHQAGHIIKQLDELCPECGGNLQLKQGNYGIFIGCSHYPECHFIVHEEAEVEEEFDCP
                     ECKQHKLIARKGRSGKIFYGCIGFPNCKFTLATRPINHLCEKCGCELATIKKIKGKQF
                     TICANKSCQHQTELKPNE"
     gene            complement(40047..40358)
                     /locus_tag="PXA33_000048"
     CDS             complement(40047..40358)
                     /locus_tag="PXA33_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005724113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HDL5119434.1"
                     /translation="MNREKITFFSRFEADILSGKKTITIRDKSESYFQPNQELAVFTN
                     ETDRFFANIKVLSVTPIVFDELNEQHAKQENMSLAELKQVIREIYPNDNAFFVIEFTL
                     I"
     gene            complement(40351..40827)
                     /gene="recX"
                     /locus_tag="PXA33_000049"
     CDS             complement(40351..40827)
                     /gene="recX"
                     /locus_tag="PXA33_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HDL5119435.1"
                     /translation="MTTSTTKTNKYTAVNYLLYLLSKRDYSEQELRQKLKQKEYELTE
                     IDAAIEKAQANQWQSDERFCTTFIRYRSMQGIGPRRLKQELKLKGVKDWLINQELENA
                     EVDWFELAEQIFEKKRPLVWDLKAKQKMWRFMVSRGFYNDHFSHLMALDYSSDEYE"
     gene            complement(40886..41992)
                     /gene="recA"
                     /locus_tag="PXA33_000050"
     CDS             complement(40886..41992)
                     /gene="recA"
                     /locus_tag="PXA33_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HDL5119436.1"
                     /translation="MAEKKSQKNTPVKQIDPEQKEKALAAALAQIEKQFGKGSIMKLG
                     DTQALDIEAVSTGSLGLDSALGIGGLPMGRIVEIYGPESSGKTTLTLSVVAQAQKNGK
                     TCAFIDAEHALDPIYARKLGVDTDGLLISQPDNGEQALEICDALVRSGAVDVIIVDSV
                     AALTPKAEIEGDMGDSHMGLQARLMSQALRKLTANIKATNCLVIFINQIRMKIGVMFG
                     NPETTTGGNALKFYASVRLDIRRSGVVKDGDEVIGSETKVKIVKNKVAPPFREVQFDI
                     MYGEGIARMNELLILAESHGFINKAGAWFSYEGEKIGQGKNNAIKWLKEHPEVASKIE
                     QDIRNLLISNPTFTATPDSENADNADDEFSEEEL"
BASE COUNT        12297 a         8194 c         9041 g        12507 t
ORIGIN      
        1 ttgatcacag atttgagaac ttaatgtggc ttttaaaaat aggtggggga ccattgttta
       61 tttttaagta aagctgcggc tcaatagaag cgattgcgtt agcgcaatca tattttttaa
      121 cccaaaagaa aagtagtgca aacaatatga ttacaatcaa aaaaggctta gatcttccga
      181 ttgcgggaaa acctgagcaa gtaatccata acggtaatac cgttactgaa gttgctatgc
      241 ttggtgaaga atatgtgggt atgcgtcctt caatgaaagt gcgtgaaggc gatgtagtaa
      301 agaaaggtca ggttcttttt gaagataaaa agaatccggg cgtggtattt actgcccctg
      361 caagtggtac cgtaacggca attaaccgtg gcgagaagcg tgttcttcaa tcggtagtaa
      421 ttaaagttga gggtgatgag caaattacct ttgctcgtta tgaagccagc caattagctt
      481 cattaacttc agagcaagta aaacaaaacc ttgtagagtc aggcttatgg acggctttcc
      541 gtacacgtcc gtttagcaaa gtgcctgcat tagatgcaga accttcctct atttttgtca
      601 atgcgatgga taccaatcca cttgcagcaa atccggaagt gattattgca gaacaccaac
      661 aagctttctt tgatggctta actgtattaa gtcgtttatt tgaaagtaaa aaagtgattc
      721 atctctgtgg cagtgcagac tcaaatattt taaatgcggc acaaagccgt cagatcaata
      781 atgtggaaac taaccgcttt aaaggtccgc accctgcagg tctttctggt acgcatattc
      841 actttattga cccggtaggt gcaactaaac aagtttggta tttaaattac caagatgtaa
      901 ttgctatcgg taaattattt acgaccggtg agctatttac tgaccgtgta gtcgcattag
      961 ccgggccaca agtgaaaaat ccacgtttgg tgcgtactcg tttaggggcg aatctttcac
     1021 aattaacagc caacgaatta aatgcgggtg aaaaccgtgt catttcaggt tctgtgttaa
     1081 gtggtgctat cgcaacggga gtacacgact atttaggtcg ttacgcatta caagtgtctg
     1141 ctttattaga agggcgtgag aaagcgttct tcggtatgat tgctccttac tctgataagt
     1201 attcaattac gcgtactact ttagggcatt ttgcgaagaa attatttaac ttcacaacag
     1261 cagttcacgg tagtgagcgt gcaatggtac caacaggggc ttatgagcgt gtaatgccgt
     1321 tggatatcat tccaacactc ttattgcgtg atttagcagc tggagataca gactccgctc
     1381 aagctttagg ctgcttagag ttagacgaag aagatttagc gttatgtact tttgtgtgtt
     1441 ccggtaaaaa caactgggga ccattattac gtcaagcgtt agataagatc gagaaggacg
     1501 gttaaaaatg ggtttgaaaa atctttttga aaaaatggaa cccgctttcc ataaaggtgg
     1561 aaagtatgag aaatggtaca cgctatttga agctgcctat acgattttct atacaccggg
     1621 tacggtcact cgtaaagatg ctcacgtgcg tgatgcgatt gactctaagc gtatgatgtt
     1681 aattgtatgg ttagcgttat tccctgcgat gttctacgga atgtacaatg ttggtgcaca
     1741 gggtattcta gcggcactca aaatgggaac attaacggat ttagttgcta acaactggca
     1801 ctacagctta gccgatagtt taggctctgt agctgatgca ggctggggaa ctaaaatgtt
     1861 gctgggggca acctatttcc taccaatcta cttaacagta tttgcagtgg gcggtttctg
     1921 ggaagtggtg tttgcaatgg tgcgtaagca cgagatcaac gaaggtttct ttgtaacctc
     1981 tatcttacta gcattaattg ttcctccaac attaccgtta tggcaagcag cccttgcaac
     2041 tacctttggt gttgtggtcg caaaagaagt gttcggtggc gtgggtaaaa actttatgaa
     2101 ccctgcgtta gccggtcgtg ctttcttgtt ctttgcttac ccgggtcaaa tttcaggtga
     2161 tgcagtatgg gttgccgcag acggcttctc aggtgcaact gcactttctc aatgggcagt
     2221 tggtggtcaa ggtgcattac agcacgttgc aaccggtgaa accattactt ggatggatgc
     2281 gttcttaggt aatattccgg gttctatcgg cgagacctct accttaatgt tgattattgg
     2341 tgctgcaatc attgtatttg cccgaattgc ttcttggcga attattgccg gtgtaatgat
     2401 tggtatggcg gcaactgcga cattatttaa tgtaatcggt tctgatacca acccattatt
     2461 ctctatgcct tggcattggc atttagtatt aggcggtttt gcgatcggta tgttctttat
     2521 ggcaaccgac ccggtatcag ctgcatttac caacaaaggt aaatgggcat acggtatctt
     2581 aatcggcttt atgtgtgtat taatccgtgt ggtaaaccca gcataccctg aaggtatgat
     2641 gttagcgatc ttatttgcta acttatttgc tccaatcttt gactatttag tagttcaagg
     2701 gaatatcaaa cgcagattag cgagggtagc aaacaatggc taaatttaat aaagatagcg
     2761 ttggcggaac gctaaccgtt gtagttttat taagtttaat ttgttctttg attgtggcag
     2821 gtgcggcagt aatgcttaaa ccaacacaag atattcagaa acaacttgat aaacagaaaa
     2881 acatcttaca agcggccggt ttattgcaaa aagataccaa tgtgcaagaa acctatacca
     2941 aatttattga gccgaaaatt gtcgatttag caacaggtga ttacgttgag ggtgtaaccg
     3001 gttttgatgc tcgtgcagca gctaaagatc ctgctcaaaa tgtagttatc gcacctgaag
     3061 atgataaggc gaatatcaaa acgcgtgcta aatatgctga agtttattta gtaaaagatg
     3121 aatctggcaa aacgactcaa gtagtattac caatgcacgg taacggctta tggtcaatta
     3181 tgtacggttt cgttgcggta caacctgatg caaataccgt taacggtatt acttactatg
     3241 aacaaggtga aacggcaggc ttaggtggcg aaattgcaaa cccgaactgg cagaaaaact
     3301 ttgtaggtaa aaaactgtat aacgagcaaa accaagttgc gttaagagtg ggtaaagggg
     3361 catctgcaga taaagatcac ggtgttgatg gtttatctgg tgcaacatta acctcgaacg
     3421 gtgttgatgg ttcattcaaa tactggtttg gtcaaaatgg ttttggtcca tatttagcaa
     3481 aatttaaagc agcggcagga gctaactaat ggcagacaat aacaatctta aaaaattatt
     3541 gttatcgcca attgtcgata acaacccgat cgcattacag atcttaggta tttgttctgc
     3601 gttagcggta acgactcaat tacaaacagc agtagtaatg gcgattgcgg taagtttagt
     3661 tactgcattc tcaagtatgt tcatttcaat gattcgtaac tatattccaa atagtatccg
     3721 tatcattgtg caaatggcga ttattgcatc attagtaatt ttggttgacc aaatcttacg
     3781 cgcttatgct tacgatctgt caaaacaatt atcggtattc gtaggcctta ttattaccaa
     3841 ctgtatcgta atggggcgtg cagaagcatt cgcgatgaaa tcaggcccgg ttgaaagttt
     3901 tgttgatggt atcggtaacg gtttaggcta cggtgcaatg ttaattatcg ttgccttttt
     3961 acgtgaactt atcggttcag gtaaaatttt cggtatcacc attcttgaaa ctgttcaaaa
     4021 tggcggttgg tatcaagcaa acggtttatt cttattagca ccaagtgcgt tctttattat
     4081 cggatttgtg atctgggcga ttagaacgtg gaaaccggaa caagtggaga aataagatgg
     4141 aacatcttct gagtatcttt gttaagtcca tatttattga aaatatggca ctttctttct
     4201 tccttggtat gtgtacattc cttgcggtgt ctaagaaagt ttcaactgct tttggtttag
     4261 gtattgcggt aattgtagta cttggcattg cagttccagc taaccaatta gtttataccc
     4321 acgtattaaa agagggtgca ttagtagaag gtgtggattt aagcttctta aactttatca
     4381 ctttcatcgg ggttattgcg gcacttgttc aaattctgga aatgatttta gacaagtttt
     4441 tcccggcact ttatagtgca ttaggtatct tcttaccact tatcaccgta aactgtgcga
     4501 tttttggtgg tgtatcgttt atggtacaac gtgagtataa ctttgtagaa tctgtggttt
     4561 atggcgtagg tgcaggtaca ggctggatgt tagccatcgt tgcccttgca ggtttaaccg
     4621 agaaaatgaa atattccgac gtgccggcag gcttacgtgg tttaggtatt acctttatta
     4681 cggtaggctt aatggcgtta ggctttatgt cattctcagg cattcaatta taaggagcat
     4741 atcgtggata gtaattttat tttcggtatt atcgcattta cggctctggt attagtgctt
     4801 gcggtgatca ttcttttcgc taaatcaaaa ttagttgatt caggtgatat taccatctca
     4861 atcaacaacg atccggaaaa aggcattacg ctaccggctg gtggaaaatt actcggtgct
     4921 ttagcaagta aagggatttt cgtttcgtca gcttgtgggg gcggtggctc ttgcggtcaa
     4981 tgtaaagtac aagtgaaatc aggcggtggt gaaattcttc caactgagtt atcgcacatt
     5041 tctaagaaag aagcgaaaga aggctggcgt ttagcttgtc aggtaaacgt gaaatcttca
     5101 atggacgttg aacttccgga agagatcttt ggtgtgaaaa aatgggaatg taccgttatc
     5161 tcaaacgata acaaagcaac cttcatcaaa gagcttaaac ttcaaattcc tgaaggcgaa
     5221 gaagtaccgt tccgtgcggg tggttatatc caaattgaag ctgaacctca taccgttcat
     5281 tataaagact tcgatattcc aaaagagtac cacgaagact gggataaatt tgacttatgg
     5341 cgttatactt caaaagtgga cgagcatatt attcgtgcat actcaatggc ttcatatccg
     5401 gaagagaaag gcattattat gcttaacgtg cgtattgcta cacctccacc acgcaatccg
     5461 gacgtaccgc cgggtcaaat gtcttcctac atttggtcat taaaaccggg tgataaagta
     5521 acaatttcag gtccattcgg tgagttcttc gctaaagata ccgatgcgga aatggtcttt
     5581 atcggtggtg gtgcaggtat ggcaccaatg cgttcacata tcttcgacca attaaaacgt
     5641 ttaaaatcta aacgtaaaat gtcattctgg tatggtgctc gttctgagcg tgaaatgttc
     5701 tatgtggaag attttgatgg tcttcaagca gaaaacgata acttcaaatg gtatgtggca
     5761 ctttctgatc cattaccggg cgacagagaa gattacttcc gtggctttat tcataacgtg
     5821 ctttatgaga actacctcaa aaaccacgaa gcacctgaag actgcgaata ctatatgtgt
     5881 ggtccaccgg tgatgaatgc tgcggtaatc ggtatgctga agagtttagg tgtagaagac
     5941 gaaaacatct tattagatga ctttggtggt tgataaacct taacttctga ctacaagcgg
     6001 tcaaatttgc aaaattgacc gcttgattta atttatatta ttaagttttg gtaatttaat
     6061 aatgtgaatt ttaaggggaa gcaaatggaa caactggata ttacggtact tgaaaatgcc
     6121 ttcaattccc ttgagaaaac gattgtcagc cttgagaatc aagcgtggtt taaccggcaa
     6181 gaagcaatta ttcaagacac tttgattgca ggagcaatcc aaaaatttga atttgtttac
     6241 gaattgtcga taaaaatgct caagcgtcag ctcaaaaaca ttgagtcaaa tgacactgat
     6301 attgatagca ccgagttccg tgatattttg cgaatggctg ctcaatatgg gctgattgaa
     6361 tcaccggaag tttggctgga ttatcgcaaa atgcgaaata ttacttcgca tacttatgat
     6421 caaaataaag cacagcaggt ttataacgga atttcaaact ttctggaaag tacgtatttt
     6481 ttagttaaac agctccaaaa acgcaatagc tatgattgat ttaactgagt cacaacgcca
     6541 acaggtgttg gatattttca aaaaatactt gcctgataca cccgtttggg tttttggctc
     6601 tcgcattaaa ggcacagcca aagcctattc ggatcttgat ttagcattaa tcaccaaaca
     6661 accgctcaca attcggcaac aaacagagtt agagcttgcg tttacggatt ctgacctacc
     6721 tttcacagta gatctgattg actgggcaag ttgcaacgaa tcgtttaaac aaattatttt
     6781 acagcgatat gaagtgctta tttaatcgct aaatatgcta taatttcccc aatttttaac
     6841 ttttgtgaca ccaatgaaaa aattttttct tcttttaatt gcttttttta ctttaagtgc
     6901 ttgtgaaaaa gcccctgagc aaattacgct gtcaggcaaa accatgggaa cgacttacac
     6961 cgtaaaatat attgataatg gtgaagtgga gaatttgatt tcgcctgaaa aaatgaaaaa
     7021 tgagctggat ggcttattga ttgaggtgaa taatcagatg tccacctacc aacaagattc
     7081 ggaaattagc cgttttaatc aagcaaagca ggcaaatcaa gcggtcgaaa tttcgcagga
     7141 ttttgcaaaa gtggtacaag aggcaatccg tttaaataaa gtaaccgaag gggctttaga
     7201 tgtaaccgtt ggtccattgg tgaacttgtg gggcttcggg ccggataaac gcttaaataa
     7261 agtgccgtct gatgaacaaa ttgcagagcg ttctgcttat gtcggcattg ataaaatttt
     7321 gcttgaaatg agtagtaaac ccaagctcac caagtcagca gctaatcttt atctagattt
     7381 atcgtctatt gcaaagggtt tcggtgtaga taaattagcc gagcatcttg aaactatagg
     7441 tgtgagcaac tatttagttg aaattggtgg cgaattacga ggcaaaggaa aaaatttaca
     7501 aggcttagat tggcggattg caatcgagaa gccggtaatg acacaaggtc aagccgcaca
     7561 aattaccgtg ccgttgcaca atttggcgat ggcaacttcg ggcaattatc gtaattattt
     7621 tgaagatgaa aaaggcaacc gtctttcaca tattatcgac ccaaaacagt tacgtccggt
     7681 aaaccataat ttagcctcta tcaccgtatt tgccccaact acaatgacgg cagacggctt
     7741 atcaaccggt ttatttgttt taggagcgga aaaagcatta gcagtggcag agcgtgaaaa
     7801 attagctatt tttttaattg ttaaaaacgg cgataaattt gagacacaaa tgtcgagcga
     7861 atttagcaaa cttattcaat cacaatagtt ggagtgaaaa atggaaactg tattattaac
     7921 ctttggcttt tttgtagccg taatttttgc gatgtcgatt ggttttatcg tgaaaggcaa
     7981 aaccatcaaa ggcagctgtg gcggtatcac cgctttaggt atgaaaaaaa tgtgcgactg
     8041 cgaagaacct tgcgataacc taaaatctaa aatcgcagac ggcacagccg accctgaaga
     8101 agtggcacgt tttaataaag agccacaatt ttatgaagtg aaataatatg aatagagata
     8161 ttcgctggaa acagcggttt gataactata aaaaatcagt aagttacctt caagcagaag
     8221 cagaaaaata cgctgatacc gacattgatg taattaaaaa aggaattatc cagagttttg
     8281 agattacaca cgagttagcg tggaagttaa tgcaagatat tttaaaatgg gaaggtgaag
     8341 ttgatattta tggttcaaaa acggcttctc gtttggcttt taatcgtggg ctaatcactc
     8401 agggcgaagt ttggctagaa atgataaaaa gccgcaatat aacagttcat actgacgatg
     8461 aaactatttt aactgaagaa tttaataaaa ttatcaaggc gtatctgcct ttatttattg
     8521 aatttgaaca gcgagtagaa acactatgcc aaactttgga ctaagcgaac gagcgacgaa
     8581 aactattacc gatattcttg cgaaataccc tgaagtggag caagccatta tttacggctc
     8641 gcgtgctaaa ggtaattatc gtgaaggctc agatgtagat cttactttta aaggaaaaga
     8701 tcttacttac gatattgtca aacgcatttg gggtgattta gacgattccg actcgcccta
     8761 tttatttgac ttgtcgattt atcatcaatt aaccaatgcc aactttattg aacatattga
     8821 tagagtagga aaagtctttt accaaagaga actctaattt cttaataggc aagcggttca
     8881 ttttgcaaaa ttttttgcaa atttcaccgc ttgttgtttt tattattaac caatgtgctt
     8941 cgccttaacc gaagttacgg gagatttatg acaactcaat taacctcaaa aacctacgac
     9001 actcatttcc ctaaattaac cccagaacaa cttgctgaaa acagcaagaa aaaagtcatt
     9061 atcggtatgt ccggtggggt ggattcctcg gtttccgctt ttattcttca acagcagggc
     9121 taccaagtgg aaggcttgtt tatgaaaaac tgggaagaag atgatgatac ggattattgt
     9181 accgccgccg ccgagcttgc cgatgctcaa gcggttgccg ataaactggg tatgaaactg
     9241 cataaaatta attttgcagc ggaatattgg gataatgtat ttgaatattt cttggcagaa
     9301 tacaaggcag gaagaacgcc aaatccggat attttgtgta ataaagagat caaatttaaa
     9361 gcctttttgg aatatgcggc agaagatctt ggtgcggatt atattgcgac cgggcattac
     9421 gtccgccgta cgagcgatga tcaagatgcg aaacttttgc gtggcttaga tgaaaacaaa
     9481 gatcagagtt actttttata tgcgttaaat aaaaagcaga tcgggcagag tttattccca
     9541 gtgggtgaaa ttgagaagcc gattgtgcga gccattgccg aagacctagg attagcaacc
     9601 gcaaagaaaa aagattctac cggcatttgt tttatcggtg agcgtaaatt taaagatttc
     9661 ttagctcgtt ttctaccggc tcaaccgggg gaaatccgca ccgttgaggg caaattagtc
     9721 ggtcgccatg atgggctgat gtatcacacg cttgggcaac gcaaaggctt gggtatcggt
     9781 ggggtgaaag ggctaagtga agatccgttt tatgtggtgg agaaagactt aattaacaat
     9841 gtattgattg tggcacaagg acacgataat tcagccctgc tttcaagtgg cttaattgcg
     9901 aaacagctca cttgggtgga tatgcagccg ttgcgtgaaa atttacgctg cacggtaaaa
     9961 acccgttatc gtcaagcaga tattgcgtgt gaaatccaag tgattgatga tgaaaccatt
    10021 cgagtgatgt ttgatgaacc gcaaattgcc gtgacgccgg ggcagtctgc cgtgttttac
    10081 ttaggtgaag tttgcttagg cggtggcatt attgagtcac aattaaaata gttataaata
    10141 aatattgaaa tattttgcat atttatgcaa aataagcgaa aagttaaggc ggtaaaacgc
    10201 ctttttcatt ttgatcgata caaagaataa tatacaagga acagactgtg gcactttggg
    10261 gtggacgctt tacacaacaa gcagacgcaa aatttaaata ttttaacgat tcattacgtt
    10321 ttgattaccg cttggcgatt caagatattg aaggctcaat cggctgggca aaagccatta
    10381 cttcggtagg tattttaacg gtagcagaat tagaacaact aatcgcagca ttaaaagagg
    10441 tgagagcaga ggtagaatct aaccttacca tcatcttaaa agatgatgcg gaagacattc
    10501 acagctgggt tgaatctaag ctgattgaaa aagtgggcga tttaggtaaa aaattgcaca
    10561 ccgggcgtag ccgtaatgac caagtagcgg tggatattaa aatgtggtgc aaggtgcaag
    10621 cggtggcatt gcaagaaaga atccgagctt tgcaagagcg tttggtttcc gttgccgaag
    10681 aaaatcaaga gtcggtgatg ccgggctata cccatttaca acgagctcag cctgtgacct
    10741 tcgctcattg gtgtatggcg tattatgaaa tgctagaacg ggatttcagc cgtttgagtg
    10801 atgctaataa acgtatgagc acttgcccgc ttggttcagg ggcattggct ggtacggctt
    10861 acggcattga tcgtgactta ttagcccgtg atttaggctt tgaagaagcc acacgcaaca
    10921 gtttagacag cgtgtcagat cgtgaccata ttttagagct gctctctact gcctcgatca
    10981 gtatgatgca cctctctcgc tttgctgaag atttaatcat ttttaatagt ggcgaatcag
    11041 gcttccttga aatgtcggat cgtgtgacct caggctcatc acttatgcca cagaagaaaa
    11101 accctgatgc ttgtgagctg attcgtggga aatccggtcg ggtatttggg gcattaaacg
    11161 gtttattaac tacactcaaa gggttaccac ttgcttataa taaagatatg caagaggaca
    11221 aagagggtat ttttgatgcg ttggaaactt ggcaggcttg tttagaaatt gcagaacttg
    11281 tactggttga tattcaagtg aataccgaac gcacccgaga agccgctcag caaggttatg
    11341 cgaatgcgac cgagttggca gattatttag tggcgaaagg cgtgccattc cgtgaagctc
    11401 atcatattgt tggcgaagcg gtggtttacg caatcagcaa aaaagagcca cttgaagcat
    11461 taagcattaa cgaattccag caattccatc attcgattgg cgatgatgtt tacccaattc
    11521 tctcgttaga atcttgctta gaaaaacgtt gtgccaaagg tggagtgaac ccacaacgtg
    11581 tggcagaggc gattgcggta gccaaagcca atttagcttc aaagtaaaac cggatacaag
    11641 cggtcatatt ttgcaaaaaa attgaaaaat atgaccgctt gccgattaaa ttttcaattt
    11701 tttacgcatt aagattgcaa aataggcatt acctctttaa acttccgaat aatttttgaa
    11761 ttttatttct actactttct ttaaagagtt ttatatgacg gaatcagtta attctactca
    11821 agctggtaca gcactttctt tagggcaaca attaaaagcg gctcgtgaag cgttaaatct
    11881 ctctattaca gatgttgctc aaaaaaccaa cttaaaaaaa tcacatattg aatcacttga
    11941 aaatgatatc tttattctac aaaatgttgc cccaactttt gtgcgtggct atgtgcgtaa
    12001 ttatgtgaaa tttttgcgtt tacctgaatc ccttatttcg agcgttaatt atggtgaagt
    12061 cactattcca aaagaagtca ctaaagtttc ttcagtaaaa ccttcatcaa attcacaagg
    12121 taaaacctta aagtatttaa cggtattagt attgcttgcg gcattaggaa tgacattact
    12181 ttggtggtgg caagggtatc aaaaagaaca ggaaaatcgc gagcaatttg ttagctctgc
    12241 accagttgct gaagtggcga ataccgctac aaataatgat gcttcagcac aaacttccgt
    12301 gcctgttcag caacctattc aggtttcaga gcagcctgct caagtagcag aaacggtagt
    12361 tgcagcagaa ccggcattaa ctgaaaaagt agagcctaca caagcagcat catcacctgt
    12421 aacacaagaa gatgttaaag tggtagaaga agttaaacaa gctgatgcca ccactgcaca
    12481 ggttgaggca gttaatgttt tacagcaatc tcaatcggaa caagccccta cagaacagcc
    12541 tgttgcggta acgaatgatg agcttcgtat cgaaattacc ggttcacaaa gctggattac
    12601 agtgcggggt gctaaaaata aacgtttagc agaaaaatta tataacaatg gtgagacttt
    12661 aagtttcaat gagaatgagc aatatcgttt aaccattggt gcgcctgcca atgtaaaagt
    12721 gtactataaa ggtcaagaag taccattgaa aattgatggg cgtgtagctc gtattcattt
    12781 accgttacag taagcttgat ttaacgatga aacgcacgta aagctagcat ttaacgatag
    12841 ctgtctttac gagcgttttc tgtttttata aattttcaca attagctaaa aatatgatac
    12901 atcaatcacc tattaaacgt cgggtatcga agaaaatcta tgtgggtaat gtgcctgtag
    12961 gcggcgatgc accgattacg gttcagtcaa tgaccaacac ccgcacaacc gatgtggaag
    13021 ccaccgttgc tcaaattaaa tcgctggaac gggtcggggc tgatattgtg cgtgtgtctg
    13081 tgccgacaat ggacgctgct gaggcattta aagcgattaa acaacaagtg agtgtgccat
    13141 tggtggcgga tatccacttc gattaccgca ttgcgttgaa agttgccgaa tatggggtgg
    13201 attgcttgcg gattaacccg ggtaatatcg ggaatgaaga gcggatccgc acggtggtag
    13261 attgtgccag agataaaaac attccgatcc gaatcggggt aaatgccgga tcattagagc
    13321 gtgatttgca ggaaaaatat ggcgagccga caccacaggc attattagaa tcggcattac
    13381 gccacgttga tcacttagat cgccttaact ttgatcaatt caaagtcagc gtgaaagcct
    13441 cagatgtatt tctagcggtt gaatcttacc gacttcttgc aaaacagatt gatcagcccc
    13501 tgcatttagg tattactgaa gcaggcggag cgagagccgg ttcggtgaaa tcggcggtag
    13561 gcttaggtct gttgctagcg gaagggattg gtgatacctt gcgtgtgtca ttagcggctg
    13621 atcctgttga agaggtgaaa gtcggttttg atattttgaa atcgctgcgt attcgctctc
    13681 gtgggatcaa ctttattgct tgcccaacct gctcccgcca agaaattgat gtgattgcga
    13741 cggtaaacga gctagaacaa cgtttagaag acattatcac gccgatggac gtttcgatta
    13801 tcggctgtgt ggtaaacggt ccaggagagg cgttggtgtc tgatttaggg gtaaccggca
    13861 gcaataaaat gagcggcttt tatttagatg gcgttcgtca aaaagagcgt tttgacaacg
    13921 caaaattaat cgaccaatta gaagctaaaa ttcgtgccag agttgcacaa caacataatc
    13981 gtatagatat tgaacaaata ggataaatat agtggcaaaa acaattcaag caattcgagg
    14041 aatgaacgac tgctcaccaa gtgagtctcc attatggcaa tgggttgaac agaaagtgcg
    14101 taatgtgctg gcaagttacg gctattcgga agtacgtatg ccgattgtag aaagcactcc
    14161 gttatttgcc cgtgccattg gcgaagtaac cgatgtggtg tcgaaagaga tgtacacgtt
    14221 ctgggataac gatgagcaat taacgctccg cccggaaggt acagccggtt gtgtgcgtgc
    14281 agcgattgag cgtggttgga tctacaataa tgagcaacgc ttatggtata tggggccgat
    14341 gttccgccac gaacgtccac agaaagggcg ttaccgccaa ttccaccaag ccggtgttga
    14401 agtgtttggc attgcaaatc cggaaattga tgcggagttg attattctca ccgcacgcct
    14461 atggaaagag ttaggcatcg atcaacatgt ttctttgcaa cttaactcta tcggctcgtt
    14521 agaagctcgt gcaaattatc gctcagcatt agtaaaattt ttagaaaatc acaccgcttt
    14581 aatgaacgaa gaagagaaag agcgtttggt gaaaaacccg ctacggattt tagataccaa
    14641 aaacgaagca ctacaaaatg tattaaacga tgccccaaaa ctgctcgatt atttagatga
    14701 tgacagccgt gaacatttta cccaactttg tgccttactt gatgcaatgg gaatccgtta
    14761 cgaagtcaat ccaaaattgg tgcgtggttt agactattac aataaaaccg tgtttgaatg
    14821 ggtcacttca gccttaggct cacaaggcac cgtatgtggc ggtgggcgtt atgacggctt
    14881 agtcgaacaa ctcggtggac acgcaaccca aggcgttggc tttgcaatgg gcttagagcg
    14941 tttagtgctg ttggtacaag aggtgaataa agagattgat ttaccaaaaa cggtggatgt
    15001 gtatatcgta ttctccggtg aaggggcaac actaaatgcc ttccaacttg cagagcagat
    15061 tcgtagcgaa ttgccacacg ttggcgtaat gacacactgt tccggcggta aattccaaaa
    15121 acaatttaag cgtgcagata aagtagaagc gcaaattgcg ttagtgattg gcgaaagcga
    15181 agtggctgaa aagcaagttg ttatcaaaga tttacgttca ggagcagaac aaatcacgat
    15241 tgctcaatca gagctgattg ctgagctatc taaacgattt taggagaaaa agatgagtaa
    15301 cgaatattta aataaaaccg aagagcagca atttgaagat gccaaaaatt ggtttaaaga
    15361 gaacgggaca ccgattttgg tggcgattat tttagcttgt gcggcaactg ccggctggaa
    15421 tttttggaaa aatcaccagc ttgaaacggc acaaaaaaca tccgcttcat accaaagcgt
    15481 gatggagagt tatttgcaaa atccaagcaa aaatcaaccg cttgttgaga aatttattgc
    15541 cgataataaa gattcaagct atgccgcttt tgcccaattt gaagtagcta aacaagcggt
    15601 cgaaaaaggg aatttttctg cggcaaaaac agcgttacag tcagttttga cttattcgga
    15661 ttcaacctta caatctatcg ctcgttttcg tttggctcaa attgacttcc aactaaaatc
    15721 ttacgatgaa gcactggcaa gtctggctca aattcaagat aaagcgtggg aattgcgtaa
    15781 gcaaattcta acaggcgata ttcttgcagc aaaaggcgat attcctgcgg cgaaaagtgc
    15841 ttatgagcaa gctaaaacga ccgcaaatcc tcaggaacaa accttaattg atgtacgttt
    15901 gaataactta taaaaacaaa gcctgattga aataaaatca atcaggcttt tttgttggtt
    15961 tagtgttgct gtgaattata aacgcattgg cataatcaca tattcggcag agctgttgtc
    16021 gcaatcttca attaagcaac tagaacttga atcaactaag ctaaaacgca cacgttggca
    16081 ttttaaggtg tttaacacat ctaatacata gctaacgtta aagccgactt ccatttccgg
    16141 gctgtcgtag gcaacatcaa tgatctcttc cgcctcttct tgctccggat tgtttgcggt
    16201 aattttgagt aaattattgc ttaaggttaa acgcacccct ctaaatttct cattagaaag
    16261 aatcgctgca cgcattaatg cccgtttaag ggtttcggtt tccgcttcta gaatgcgatc
    16321 tgcattgcga ggcagaactc ggcggtaatc cgggaatctg ccatcaatca gtttagaggt
    16381 aaatacaatg ccgttcattg taatacggat attgcttgaa ccgatttcca aacgcacggt
    16441 ttcatcgttg ctggcaacca agcgggaaag ctcaagtacg gctttgcgtg gcacgataac
    16501 cgaatgagtc aaaagctctt gatttagcac cattgtgcaa accgctaaac ggtgcccatc
    16561 tgtggcaacg gtgcgtaata aattgccttc ggtttcaaat ttcattccgt ttaagaaata
    16621 acgggcgtct tggtttgcca ttgagaattg ggtggcatca atcagacggg caagggtaga
    16681 ttgatcaatc gagaaatcca cttccggttt ccaatccatc aaattcgggt aatcacttgc
    16741 cggtaatgta gctaaactaa atttactacg tcctgctttg ataatggctt tatcttcttc
    16801 aaattgtacc gaaatcacgc tgtcatcagg taggctttta ctgatatcta ggaatttttt
    16861 agctggaatg gtaaattttc cggcaaaatc gaccgcttgt agcagttgtg cttgggtggt
    16921 taattccact tctaaatcgg taccggttaa agatagacga ttaccttcaa tttctaataa
    16981 aagattgtta ataacaggca aagtcggacg gctacttaat acgccacaca cctgttgtaa
    17041 aggcttaagt aattgttctc tggtaatggt aatttgcata atcattcctt catcgggcta
    17101 agatgataat ttgcgtgtta aatggatata atcttcttgg ataccgcttt cggtatcgcg
    17161 taattcatta atggttttgc aggcgtgcat cacggtagta tgatcccgac cgccaaattc
    17221 ccgaccaatt tccggcaagc tgtggttggt tagctctttt gccagtgcca ttgccatttg
    17281 gcgaggacgg gcaatgcttc ttgcccgact tttcgattta atatcggcaa cttttatgcc
    17341 gtaatattcg gccacaatgc gttgaatatt ttcaatcgta atcaagtaat caaaagaggc
    17401 aaacagatct tgcaaggttt cacgcaccgc atcaaccgta atcgggcgaa gggtcaattt
    17461 ttgccacgca atcacacggt ttaatgctcc ctccaattca cgcacattgg ttcggagttt
    17521 ttgccccatt aaataggcgg catcttcttg caaatctgct ccacgttcta aggctttttg
    17581 gcgaagaatt gccactcggg tttccaattc aggtggctca atggtcgcac taataccgcc
    17641 gcttaaacgg gatttaatcg ctgtgtcaat tttatcgata ttttttggca aaacgtcaga
    17701 ggttagaata atctgctttc tctgctcaaa taaagcattg agtagatgta aaaactcttc
    17761 ttgtactttc ggtttattgg caagaaattg aatatcgtca atcattaaca catcaagcga
    17821 gcggtagaat tttttgattt tttccgcatc gttattggaa ctgttgatcg ccttaataat
    17881 atcacgataa aaacgttcca aatgaatata tagcacccga gtacccggtg tgcgtttaac
    17941 taattcattg ccgatagcgt gtaataagtg ggttttgccc aaaccggtgc cggcataaaa
    18001 cgagagtggg ttgcaatggc tttcgcctaa attatccacc acttgttgag ccactaattt
    18061 agctaattgg tttgagcgac cttgtacaaa attatcaaag gtttgatttg gatttagccc
    18121 ggttttaaag ccggtattgc tgaattctga actctcgttt tttagatttg ggctgacctt
    18181 tggctgttct gtcggtttaa tcccaacttt taacgtaacc accaaattat catttttcgc
    18241 caaaaaacgg gcaagctcgg taatttccga taaaaattta tcttttaccc agttttcaac
    18301 aaattgattt tgtgcataaa gtgtaagttc cccattctca gaactcgctt gcagaggacg
    18361 cagccaagtg ctgtaatcag ttggtgagac tttcgtttgt aagtgattaa ggcaatcaga
    18421 ccaaagggaa gacacaattt gctccaaaat tattgttatt aaaggtcata atgatacctg
    18481 aaaaaagggg agaaatcttg caaatttttg agagatttta tgggggcttg gaagcgattt
    18541 taattggcga acaagcggtc gttttttgtt gaaatcttgc aaattgctaa caaaaaatga
    18601 ccgcttgctt agtatgatgt gtgatggagt tgattaaata tccgtattct ccggcaagaa
    18661 cccaccgctt tggtgagccc aaagtctggc gtaaacaccg ttttgagcaa gcaattcttc
    18721 gtgcgtgcct tgttcaacga tttcgccttt atctaacacg ataagtctgt ccattgccat
    18781 aatggtagag aggcggtggg caatcgcaac cacggtttta ccttccatta attgcgtgag
    18841 attttcttga attgccactt ctacttcaga gtctaacgca ctggttgcct catcgagtaa
    18901 taaaatcgga gcgtctttta gcatcactcg ggcaatcgca atccgttggc gttgtccgcc
    18961 ggaaagtttt acccctcgtt cgccaacgtg tgcatcaaaa ccggttctgc ctttggcatc
    19021 ttgcaggttt ggaataaatt cactggcggc agcttgttca acggctttga tcatctcttc
    19081 ttcagtggcg tttgggcgac cgtacatcaa gttttcacgc acagagcggt gtaacagcga
    19141 ggtgtcttga gttactaaac caatttgtga gcgaaggctt tcttgggtaa tatcacggat
    19201 attttgcccg tcaatcgtga ttgaaccgtc ttgaatatcg taaaaacgca ataataaatt
    19261 ggtgagcgtt gatttacctg caccactacg cccgactaag ccgacttttt cccccggttt
    19321 tatagtgagg tcaaaatctt tcagtagcgg ttttttcgga tcgtaggcaa aatccacatt
    19381 ctcaaattta atttcgcctt tggttacatt taacggtttc gcatcgattt tatctaccac
    19441 attgtgtggt ttggaaagcg taatcatacc gtcttgtacc gtacctaaat tttcaaatag
    19501 acgagcaaat tcccacataa tccattgtga taagccttta atgcgtaaag ccaaagcggt
    19561 tgaggtggca atcgcccctg cagttactac gcttgaaccc caaagccata aaccgatacc
    19621 cgcagttgaa attattaatg ccatcgaggt tagattagtt acggtttcaa ttacagtcac
    19681 taaacgcatc tgtttatgca ctgtcaccat aaatccttcc atcgactctt tcgcataggc
    19741 agattcacgg ttgccgtgcg agaagagttt gacggtggca atgttagcgt aagcatcggt
    19801 aattcgaccg gtcattaaac ttctggcatc cgattgctct tgagcagctt gggctaattt
    19861 cgggacaaag tagataatgg ttgccgctaa tgagatgacc cataaaataa acggcacgaa
    19921 taaccagccg tcaagttgta ataaaatcac gctggaggtg gtgaagtaaa ccagcacata
    19981 caccaacata tcggcacaag tcataatcac atcacgcacg gaaagggcgg tttgcattac
    20041 ttttgcagac actcttcctg caaattcgtc ttgatagaaa cctaaacttt gcccaagcat
    20101 taagcggtgg aagttccaac gcaaacgcat tggaaatacc ccttgcagag attgaaagcg
    20161 aatgctgctg gcaaggtaaa caaacaccac tgcaagtagg gcaataaaaa acataccgat
    20221 aatcgtccag cctttttctg cccaaagttc tgttggcgag tatttattga cccaatcgac
    20281 taaatcgccc ataaagcgaa acagaaccgc ttcgataata ccgacagctg caactaatac
    20341 ggtcagtagt aagatataaa accgcattcc tttagtggca tcgtaaataa aaggaatgag
    20401 acctgatttt ggagttttcg gcatttcatt tggataggtt tccacccttg cctcaaacca
    20461 tttaaacagt ttttcaaaca taaatttgtg atttccgaat aaaaatgcag gggcaaatag
    20521 ccatttgccc ccgaaaaaat gtgaaaaaat tatggattaa gtaatttttc gtctaaggct
    20581 aaatcttcgt tgcgatttat accaatgccg gcagctaata cgttgcgggc aatttgatgt
    20641 gcttctttta aagaatgctc tttgtaagag ccacattgat attcattcag ctcagggatt
    20701 gccgatacat caggaatttt atgtaacgca tcttccattg atgccgtcca agctttgaca
    20761 acttcttctg ctgaaggcga tccaatcagt gacatataaa aaccggtacg acaacccatt
    20821 ggtgaaatat caatgatctc cacttgttcg ctattgaggt gatcacgcat aaaaccggca
    20881 aaaagatgtt ccatcgtgtg aatcccacgc accggtagaa tatcaatatt cggacgacag
    20941 aaacggagat cgaacacggt aatggtatcg cctttcggtg tggtcattgt ttttgcaaca
    21001 cgcacggctg gtgcgttcat tttggtgtga tctactttaa aactatctaa taaaggcatt
    21061 ttttactcca tcaatattat tccgcaattc cttgatccca agccggggct tcatcaggta
    21121 gtttgcgctg taagaaaaat cgtctgtttg gctgggcttg aggcttcacc actttagtgt
    21181 aaggggtaga gaactcaata ccgccattta ataactgcga cattgtgccg gagttcatac
    21241 ttacaccttg caggctcacg tccatagtgt aacctgaagc ttgccagaat tgggtattgg
    21301 ttctgactaa gtgcttgtat ttatcttgaa tattgacata aataagcact cgatcgccta
    21361 actcacttag ccctaaacgg ctgactattc ccacctgcat tccacggtaa agcaccggtg
    21421 cttccggctg aataccgtta gcattggtgg tttcgacaat aatggggaaa ccgttggcat
    21481 aggtggtttt taccgtatcg gtatctgcaa gaacaaattg gttttgagct tgccctgaac
    21541 cgacatcaac cgtaatgtaa ttttgtagtg ctgcatctaa atttttaaag ccggaggtac
    21601 tgatttcagg cgagataaca ttaaatttac tgccggcttt tgcaattaac ggatagtatt
    21661 tcctttgaat gtaagcggtc gcttttatct gtttttttgc attttgtagc tcaagttttt
    21721 caatttgacc tacggttaag cccatatatt taatgtccat tccttcgctt aacttactcg
    21781 catcttttgc aatcagcgtg agataagtat tgcccgaagt ggctttctcc ttacttgcat
    21841 aaagcgtttt attgcctttt gaaccgccat catcaaagct gatcgcccct tttaaggtac
    21901 gcattaatgg agctgtagtg atattcacgc ctttgagtga cacatcaaca ctcgttgccg
    21961 gctcaatcca gaaacggctt ttctcggtta atagatgacg ataatttggc tggataaata
    22021 gctcaacctc aaatttttgt tgtttcgggg ttatttttaa cacctcgccg acctgaaaat
    22081 cacgatatag caccaaagag ccttcgctga taccagataa tgaatctgcc gaaagggtag
    22141 tggtggcttt tttatctaag gtagccatgc ctgattccgc actttctata tctttataaa
    22201 gtgcatattg ttttttcggg ctaccgtcat ttttaccgtt aattaagcga acaccgcctt
    22261 ttaaccattc agatggtgaa ccggcgtgaa ctcttaaccc atctagcccc accgacatat
    22321 caatgttgga tatcgcaaca aatttgctgt tactcccaat caggttgcga taaggcggat
    22381 aaataatgac atcaaaacgt actttgtcta actctaggct acgtttgacg atttctccga
    22441 tttgcacatc gctatagtaa atcccctgat tggtatctac cccataggct tgaggagcaa
    22501 ttaagctaaa gctaagtaaa ttcggtaaac ttaatagata atcggcttcg ttttgtacca
    22561 caaattccga ttgaggttct ccttcccccg ggtgaacctc aaaatagaca ccacgcagca
    22621 gctcacctac tttcgtcagc tgctctgtgt ttaaggagaa tttcggctct ttgagcaaga
    22681 ttttagtttg ggaacgtagt aaattttcat aacttgggtc aattaataaa accccatcaa
    22741 tctgtttatg ctgccctgat tcttgctgtg gcacaccgtt gtcttttact tgcggtaatt
    22801 ttagctgcga aagcatacca atttgcacat tttggtgaaa aacaccagtc tcattaattt
    22861 ttaaattatg tgtaagcggt agttttacgt tgatttcaat tccacgttta gctgatttca
    22921 aatccggata tagctcaaat ttctgtcctt gctcggcaat ttccatatta tcaggcgaat
    22981 caaaagcaac cgcaccttgt actaccgagg caaggctgtc catattgatt ttaatacctt
    23041 gtaaatttac atcagcgcta ataccgctaa tattccaaaa tcggctctct ttttttacta
    23101 aatgagcgta tttttgatta atgacgacat caatttcgat ttttttctgg tctttggtaa
    23161 aacgataatc ggcaatatta cccactggca ctttacggaa ataaacactc gctccgacag
    23221 taatagagcc tatgtcatca gagattaaac ggatcagtaa atcgccatcg ctcacggtaa
    23281 tagccggagg atcttcttct gcaataaatt catcaacgaa atcgccttca ccgggaagta
    23341 gggtaatata gttaccagaa acaatcgcat ctaagcctga aacgccagcc aaagaagcac
    23401 ttggttgcac taaccaaaat ttagtctgtt tgcgtaaaat acttttggct tcagggttta
    23461 tttctgcttg cacttccact tcttttaaat tatcgacaaa ataaacacgc ttaacctgac
    23521 caatctgcaa gccttggtaa cgaatagcgg tttttccggc agtaatgcca tcacctttac
    23581 taaatcgaat cgtgattgtt tcaccttgct cttttaaaat ttggaaaaat agcaaagcac
    23641 caataataaa agcaactatt ggtaacagcc aaaaaggaga aattttgcga ggctgccgga
    23701 tttgggcatc tacaacagtg tgggtatttt cttccatcgt cattttaaat tagggaaacc
    23761 tatatagaac aataccgaag acccaaaggg acttcggtaa tagaaataag tcaaaataag
    23821 aaatattatt ttttctcatc acagttggtt aaattactgc atttaccata cagatataaa
    23881 ctgtggttag tcaattccat tccgtgtttt tcactgattt cacgttggcg acgctccata
    23941 tcaggatctt taaattcgaa caccttacca caatccatac aaataatatg gtcgtgttcg
    24001 tggttcatat ttaattcgaa tactgcttta ttcgcatcga aattatggcg ggttaaaatg
    24061 ccggcttctt caaattggtt taagacacga taaaccgttg ctaaaccaat gtctgagcct
    24121 ttttctaaca gaatcttata aatatcttct gctgcaaaat gttgcatttc atcacgaata
    24181 tcttgcatta atgcaaggat agtcaaacga ggctcggtta cttttaagcc tacctctttt
    24241 aaacgtttaa tatttgcttc agacatatat gccacttatt tatctttatc caaaatagtt
    24301 attatgcgag ttggtctaag cacatttctt ctttcaactg ctcgcaccat ttattaacac
    24361 gttcagtggt taattcaggc tgacgatctt catcaataca taagccgaca aaagtatcat
    24421 catcaactaa ggcctgagaa acctcgaaag tgtaaccggc agttggccaa tgaccaacaa
    24481 tcacaccacc atttggctct accacatcac gcacagtacc cattgcatca cagaaatatt
    24541 ctgcataatc ttcctgatca ccgcaaccaa aaattgcaac gactttgccg ttaaaatcaa
    24601 tctcttttag ggtaggcata aaatcgtccc aatcggcttg cgattcacca taataccaag
    24661 ttgggatccc aaacatcaga aaatcgtatg cttcaatatc ttcttttgtg cttttggcaa
    24721 tgtctcgaat atcaattaat tcagccccta attttttctg aatcattttt gaaatatttt
    24781 ctgtattgcc tgtatcgcta ccgtaaaata aacctacaac tgccattgct actacctatt
    24841 tgtgagataa ttatttagaa ttgtttcaat taactgcccg cgtgaaacac cttgttcaga
    24901 cgcctgttcc tctaataact ttactaaatt cgaatgcagt tttaattcta cccgttttaa
    24961 accggaagat ttatctcgtt ttagttggtt acgtttattg attcgaagtt gttgttctct
    25021 atcaagagga tttgtacgag gacgcccaac ttttggtaca gtcgcaaaca gatctaaggt
    25081 tatacaatct gaatcttgtt ttgccatttt gttttactat tgggctttag ctaatcctca
    25141 gttttaatga gaatgataag ctacttttaa agtgtgaact atatcaaaag ttcaatcatt
    25201 tgaaaagtaa attacaggca aaacaagcgg tgtttttagg tttaaaattt gcaaaaacca
    25261 taaattacac attatgagtt atgatttttc tgaagcgtga aattagctta taatgaaacc
    25321 gaataacgtt atgaaaaagg agatagtgtg tcgaatatcc atttagcgat tgccgctgat
    25381 tttacgctgg cagaaaaatt acttgaagca ttagcaaatc aagatttagg gattgaaaat
    25441 atttcagccg ttgagcttga gccgtttggt gaagagcaga atttacgctt tggggcaaaa
    25501 gcagtcgcac aatatgcgtt agatgaagta aattgggcgg aatttagcca tgttctattt
    25561 gccggcaaaa tggcacaagc ggaagtgttg gcagaagcgg tgaaagccgg ctgtgtgatt
    25621 ttagatttat acggcattac cgcactcatc ggcgatgtac cggtagttgt gccaagtgta
    25681 aatgatgaaa atgtcgcaaa tttgcgtgag cgaaatatcg ttgctttagc caatccgcaa
    25741 atttcacaac tggcgttagc cttaaagcca ttgttgaacg agccgttaaa acaggtgtgg
    25801 gtaacctcac ttttaccgtc tgcctatttt ggcgatgaaa aagtcaagga gcttgccgga
    25861 caaactgccc gtttgttaaa cggcattccg tttgatgaaa atgcggaacg cattgccttt
    25921 gatgttgtgc cggctaatgt gcaaggagaa gagaaaagtc tgccgttctc aaaagccttt
    25981 gaactgcaaa ttgcaaaagt tttaccaaat ttgaccgctt gtactacctt ccacagcgtg
    26041 caagtgccgg tcttttatgg ggtttcccaa atggtgtcgg tgcagtctga atatcagtta
    26101 gatgctaatg cgttaagtga agcttggaaa gccgaaaatt ggctgaattt taatgaaaat
    26161 tcagtgatta cagcggtgaa aaatggcgaa aacgaagagc aaaatgcgtt agaaatcagt
    26221 cagttattgg cgaaatccga acaggaagtg cagttctgga cggtcgccga tgaacagcgt
    26281 ttcagtctgg catttttagc ggtgaaatta ctaaaattag ttttagcgta ttaagtgctt
    26341 gcaaaagtat ttattccgtg ataaaaatcg tcaacgtccg agcaaccgat tgcttgggcg
    26401 ttgtcatttt gtttaaacac cgaaggtctt atgttagaaa aattatttca gctcaaagca
    26461 aaaggcagta atgtaaaaac cgaaattatc gccggtatta ctaccttttt cacaatggtt
    26521 tatattgttt tcgtcaatcc gtctatttta ggcgtggcgg gaatggacac ccaagtagtg
    26581 ttcgtgacta cttgtttaat tgccgctttt ggtactatcg caatggggtt attcagtaat
    26641 ttaccgattg ctcttgcacc ggcaatgggc ttaaatgcct tttttgcatt cgtagtggtg
    26701 caaaaattag gttactcgtg gcaagtgggt atgggggcga ttttcttagg ctctgtcggc
    26761 ttattcctat taactgtatt acaagttcgt tactggttta tgtctgcgat tccacttggt
    26821 ttacgtgtag gtattggtgc cggtatcggt ttattcattg ccctaatcgg ttttaaaaat
    26881 atgggcttgg tggtgccaaa tccggcaacg cttgtggcac taggtgattt gcacgacccg
    26941 aaagtgttaa tggggatttt aggctttttc atcattgttg tattagcggc gaaaggtatt
    27001 cactcaggcg tgttaatttc aattgtgatt gttaccgctc ttgccttagc ctttgacccg
    27061 gcggtgagct ttaacggcat aatgtctatg ccaccaagcc ttggggcagt tgtggggcag
    27121 gttgatattg ccggtgcttt tgatgttgcc ttactcggga tgattttctc ctttatgttg
    27181 gtaaacctgt ttgactcttc cggcacctta attgcggtga ctaccaaagc aggctttgcg
    27241 gatgaacaag ggcgtttccc gaaaatgaaa caagctctgc tagtagatag taccgctgca
    27301 atggcaggct cttatatggg aacttcagcg atttctacct atattgaaag tggctccggc
    27361 gtttcagttg gcggtcgtac cggcttaact gcggtggtag ttgggatctt attcctatta
    27421 accattttct tctcaccact tgcagggtta gtacctgcct atgcaactgc gggggcattg
    27481 gtatttgtag gaattttaat ggcatctagc ttaattgaag taaaatggga cgatttaact
    27541 gaagctacgc cggcatttat tactaccgca atgatgccgt ttacttactc catcactgaa
    27601 gggattgcat tcggctttat cagctactgc gtaatgaaag cctttacagg gcgttggaaa
    27661 gaaatcaacc tttctgtttt agtggtttca cttctcttta ttgctaaatt tgtgtgggtg
    27721 ggttaagctc tacccccctc tccctgattt ttcttctaaa cgaagaaaaa tctatccctc
    27781 tcccacaaag gggagagggt atttgattca ttatctatgt gtttcgtgta ttccgaggta
    27841 aaacaatgtc taattcagtt caaaaacttc tttttatcat caattctgct ccttacggca
    27901 atgaaacgtt tttcagtgcg ttgcgtttgg cattacaaat tcaagaacaa cataaagcca
    27961 atacggaaat taaacttttc ttaatgtcgg atgcagtcac cgcagggctg gcaaagcaaa
    28021 atccggcaga gggttatcat attcagcaaa tgctggaaat tttaaccgcc caaggggcaa
    28081 acgtaaaatt atgcaaaacc tgtactaatg ctcgagggat tacagatttg cctttagccg
    28141 atggggtaga gattggcaca ttaattgaac tggcagattg gacaattcaa gcagataagg
    28201 tactgaattt ttaatgcaaa atattgatgt tgtggtgatt ggtgccggtg cggcagggct
    28261 gttttgtgct gcccaactgg ggcaagctgg caaaaaagtg gtggtgttag acagcggtaa
    28321 gaaagtcgga cgtaaaattt taatgtctgg cggtggtttt tgcaatttta cgaatttaga
    28381 cgtaagtgcc ggaaattacc ttagccaaaa ccgccatttt gtgaaatccg ccctttcacg
    28441 ttatacccaa tgggatttta tttcgcttgt cagccactat ggcattagct atcacgaaaa
    28501 agagctaggg cagttgtttt gtgatgaaag ctcgcaacaa attgtcgatt tactacaagc
    28561 agaatgcaac aaaggcaagg ttgagattct gttacggcaa gcggtcattt ctgtggaaaa
    28621 agttgcaaat ttttttgaaa tttcgaccgc ttgtggcaag ttccaatccg aacacttagt
    28681 gattgcaacc ggagggcttt caatgccggc tctcggggca agcccttttg gctatcaact
    28741 tgccgagcag tttggtattc ctgttgtgcc accacgtgca agccttgtgc cttttacttg
    28801 gaaagagagc gataaatatt ttgccgagct ttccggcatt gcactgccga ttactgtttc
    28861 cgccaatggc aaaagtttta gcaatcagat gttgtttacg cacagaggct tatccggccc
    28921 ggcaattttg cagatttcca attattggga attgggggaa acggtagaga ttgatctgct
    28981 accttcgcag tccattaccg atatattgag cgaactacgc caatcttcgc cgaaattaca
    29041 gctaaaaacc gtattatctc gttatttacc taaaaaattg gttgaagttt ggctggaaca
    29101 gaaattgctg accgataaag tgattgccga tttgagtaaa aaagatctga ccttattaga
    29161 gcaatttatc caccattggc atttcttgcc aaacggcacg gaaggctatc gtacagcgga
    29221 agtaaccaaa ggcggtgttg ataccgacta tatttcatct aaaacgatgg aagcgaaaaa
    29281 tacagcaggg ctttatttta tcggcgaagt gctggatgtt accggctggc ttggtggtta
    29341 taatttccaa tgggcgtggt catctgcttt tgcctgtgca gaagggatta ttgaaaaaag
    29401 caaataacta aaaaagcaaa taactaaaaa accaaacaaa aaggcgaaca caattgttcg
    29461 ccttatttta tgctgaatta tcttgcacgg aaaataatgc gtgctttgct taaatcgtat
    29521 ggagtcattt caaccgttac tttatcaccg gttaaaatac gaatgtagtt tttacgcatt
    29581 ttgcctgaaa tatgtgcggt aacaatatga ccgttttcta attccacacg gaacattgta
    29641 tttggtaagg tttctaaaat cgtaccttgc atctcaatgc aatcttcttt agccatttat
    29701 tcctctttaa agttttaatt tttatatgat gccattcggc gaaaaacggc ggatattata
    29761 cgcttattcg ctcaaattac aatcgtttat ccatttgagt gtaaactatt tacgcttaat
    29821 ccaacggcga cgtgagtgaa aacgctgggt ttgggttttt acttgactca taagtggttt
    29881 tgggcttatc tgtacatgat gaattttacg acgaaaatag tagttaatgc tacttgccaa
    29941 caatatacct gtgagtagca aaataattat ttccccataa agttgatgaa attgttgctc
    30001 tactcgtgct ttagtagttt gcccatattc tgcatcaaac gtgacgcgta aaaagccaac
    30061 caacgcctgt tgagaaaata ttggctcaac aatttgctga gttttggcag attgctctga
    30121 atcgagcaat acttttaaat ctttcgcatt ttggctttga gccagtagca cgccatttgg
    30181 tgaatagata ttggcatcaa taataaaatc ttctttggca aaactatcca acgcatcaac
    30241 cagctcatcg gttttggcat ttttaactaa caaaaacgaa aaaatatttg cttgctggcg
    30301 aaccaatagg tgtgaaaggt ttgatacttg attaatgctg gccaattgtg agcctagctt
    30361 aaattggttt acgcccatta aaataaccgc cacgatccca cagcagagta gcgtaatccc
    30421 tgccagcaaa agcgttttcg ttattttttc acgactaatg tacatcgaaa ttccctcaaa
    30481 atgatgcgtg attgtagcac gcttttctgt tagattagtg cagtaaattt aaacaaccta
    30541 aaataagaca atgacaaaca caatttttat ctattcaaaa accttaaacg agcgtgaaat
    30601 tgaacagttt tcacaacaaa cggcagcaat tttgctgaaa caagcggtct atttagacta
    30661 caaaattgca tttttttcca caaatttaac cgcttgcgag ctgcgtgaaa cggcacagaa
    30721 aatcaatgcg gatattagcg atctcactat tattccaacc ttaaaccaag gcggtttgct
    30781 attaatggat atggattcca ccgctattaa aattgaatgt attgatgata ttgccaaact
    30841 tgccggcacc ggcgaggtgg tgtccgccat tactgccagt gcaatgcggg gcgagctgga
    30901 tttcgagcaa agtttacgca agcgagtcgg cacgttggaa aatgcatcgg aaagcatttt
    30961 gcaaaaagtg cgggaaaatc taccgcttat ggacggcttt gagcagatgg ttgctattct
    31021 taaggaacac ggctggaaat tagcgattgc gtccggtgga tttgattact tcgccgatta
    31081 cttaaaacaa aaatacgggt tgaattttgc ggtctctaac caactggaaa ttattgacgg
    31141 taaattaacc ggcaaggtgt taggcaaagt agtggatgct caacataaag ccgatacctt
    31201 acagaattta gcgacaacct ttaatattcc accaaatcaa tgggtggcag tcggcgatgg
    31261 ggcaaatgac ttaccaatgt taaaaaccgc cagtttaggc gttgctttgc acgctaaacc
    31321 aaaagtgcaa gcacaagctg attttgtggt aaacttcggg gacttaacgg cattatgttt
    31381 attacttaat gccaatcatt tatttcaaca ttaattgaga ggaacaacaa tgccatcatt
    31441 tgatattgta tctgaaatca cgatgcacga agtgcgtaat gcggtagaaa atgccaatcg
    31501 tgtattaagc acccgctacg atttccgtgg ggtggaagcc gttattgagt taaatgaaaa
    31561 aaatgaaagc attaagctca cgaccgaatc ggatttccaa ttagagcagt taattgagat
    31621 tttaatcggc tcttgtgtaa aacgtgggat cgagcatagt tcattagata tccctacaga
    31681 aagtgagcat cacggtaaac tctattccaa agaaattaag ctcaagcaag ggattgagtc
    31741 agatatggcg aagaaaatta ccaagctgat taaggattct aaaatcaagg ttcaagccca
    31801 aattcaaggc gagcaagtgc gtgtaacggg caaatctcgt gatgatttac aagccgcaat
    31861 tcagctagtg aaaggggcag atttagggca gccattccaa tttaataatt tccgtgatta
    31921 gcaattcctg caattaatga tgacaagtgg aaaaattcag caaaatttga ccgcttgcat
    31981 taataattca acataaaagg aagagatgat gagcgagcaa acagaaaaga aaggctggtt
    32041 taaaaaagcc cttgagaaat atgatgcctt ttgcgatgag ctaggggtaa atcaaggtgc
    32101 ttgccgaggt tgtgtccctg tggtgaagtt tgaccctgaa ccggaaaaag agcagccaga
    32161 aaaatcagaa aataaaacat agaaaaaatg cacaatggta agatacattg tgcatttttt
    32221 ataggatttt taattggcgt gatgatgggc aaaatattgc ttaactccgc catctttctt
    32281 gcaaggattc tgaatatcag tagagtaact accgttaaaa caaagagcag tagcattcga
    32341 tggtttaatc ttttcctgtt tagttagggc tggcggttga gtattggttt cactaatata
    32401 gatcgtttta ggttgagaat agtctatatt tttgtcgcca tttacgactt gaacgggggt
    32461 gttcgagctg caagctacaa ccgatgctga agccaatgta agtataatga tatggtgttt
    32521 tttcatgggt aatttccgtt tttatatttt taattgagag aatgttaaaa agggctgatt
    32581 tctcaaccct taaagttatg ccttaagcat ttttattaaa agtttgtcct aactgaattt
    32641 catcatttaa tacaggaatt aactgctcta gttgatcttt ttcaaattcg ctgagattgc
    32701 cgataggtag aactttttcc acaccatttg tacctaaacg aatcgggtaa gcaaagaatg
    32761 ccggagaggt ttcgccattg gcattatcta cataagcata acgaatgaca tcttccccga
    32821 ttaaggctct cactaaacca agtgcaaatt ctgctcctgc ttgagccata gataaggttg
    32881 ctgagccacc acctgctttt gcctcaacca cttcagtacc ggcgttttgg atacgatgag
    32941 ttaattgctc gatttgttct tgagtgaact caagttttaa gccatttact gttgcttgtg
    33001 aaagcagtgg aagaatagtt gtgccggaat gtccgccaat cacaggcact cttacatatt
    33061 tcacatttac gtctttcgct tcagccacaa aggtattcgc tcgaataaca tctaatgttg
    33121 taacaccgaa aagtttattt ttgtcataca caccggcttt tttcaatact tctgccgcaa
    33181 tcggaataat ggtgtttacc gggttagtaa taattgcaat gcaagcttta gggcaaactt
    33241 tcgctgcttt ttcgactaag ttatgcacga tagtggcatt cgtattgaat aaatcggcac
    33301 gtgtcatccc cggcttacgg gcaacaccag cagaaataac gaccacatcg gcatctttta
    33361 gtgcctcagt cggatcttca cctgcaaaac cggttacttt tacatcagtt ggaatatggc
    33421 ttaaatccac tgcaattcct ggagtaacag gggaaatatc gtaaagagat aattcagatt
    33481 tagccggtaa attgagtttt aataataagg cgagtgattg accgattcca cctgcagcac
    33541 ctaaaagggc aactttttta ctttgacaca taaaatttct cctaaatgtt atggaagttt
    33601 agaccaattt agtctaagcc aattttttgg gcgattcaaa cggcaaatct catttttgag
    33661 acatagagca aatttttagc tgatttttat gggttttatt gatttatttg cataaataag
    33721 caagaattat gaatattttt aagtttttgt gaataagagt gtgaaagtgg acaagataga
    33781 tagtttatca gaagcgttta aatcgctgtt aaaggaagaa aaattcggct cgcaaagtga
    33841 aattgttgcc gcactacaag agatgggctt tgataatatc aaccaatcca aagtttctcg
    33901 aatgttatca aaatttggtg cagtcagaac ccgtaatcct aaaatggaaa tggtgtatca
    33961 gctaccagtc gaattaagtg taccgactac ctccagccca cttaaaaatt tggtggtaga
    34021 tattgatcat aatgatgcgt taattgtggt caaaaccagc cccggtgcgg ctcaactgat
    34081 tgcacgtttg ctcgattcaa tgggtaaagc cgaaggtatt ttaggcacta ttgctggcga
    34141 tgatacgatt tttataacgc cggtacgcaa tgtgcaaatt gaaaaattgg tgaagaatgt
    34201 gacacagtta tttgaaacat cattatagaa taagtaaagg aggacttaat gttctcctta
    34261 ttttttagga gattattatg cacattttta taacaggtgg tacgggtttt attgggcgtg
    34321 aattaatcca gcaattaaaa tctgaaaatc accactttac acttcttact agagatacag
    34381 aagcgaaaag tgatgattta cagattaatt attgctattc acttgatgaa cttgttgatt
    34441 taaatgattt tgatgcagta attaaccttg ccggtgagcc gatttttgat aaacgttgga
    34501 cagacgaaca aaaacagaaa ttgattgaaa gccgggttaa cctaacgaat aagctcagtc
    34561 aattaatgaa tgcaagctct aaccctccgc atacttttct ctccggttcg gcaatcggga
    34621 tttatggcga tttgcctcct tctgcaaatt tttatacaga atcgaccgct tgttatcaag
    34681 attgctttac caaccggctt tgccaacagt gggaagccgc tgcactaaaa gccgcaagtg
    34741 ataaaacccg agtgtgttta attcgtaccg gcacggtatt ttcgcccgaa ggcggagcat
    34801 taaagaaaat tttaccgctt tatcgatgcg gattaggcgg aaaacttggt tcaggcaaac
    34861 aatattgggc gtggatcagc cgccgggatc acgtacaagc ggtcattttt ctgctcaaaa
    34921 atgcaaattg ttcgggtgct tataattttg ttgcaccaaa tccggtgaga aatgaagaat
    34981 ttaatcgtat tttagcgaat gctgttaatc gcccggcatt ctttactgtg cctgaatttg
    35041 ccttgaaatt aggcttaggc gaacgagctt gtttagtctt agataatcaa cagctcattc
    35101 cggaaaaact actcgcagcc agttttgtat tccaagatcc gattttaacc gagaacgttt
    35161 tcttaaaccc ataatttagg ctataatgct gtataaaagc acagattaaa aggtgattat
    35221 ggatttctca ttattattag acggtttaaa tgacaagcag agggaggcgg ttgctgcccc
    35281 gattggtaat tatttagtgt tagccggggc gggttcgggc aaaactcggg ttttaaccca
    35341 tcgcattgcg tggctgattg gggtggaaaa tgtgccggaa tccaatattc tggctgttac
    35401 ctttaccaat aaagcggcgg cggaaatgcg acatcggatt gagtacactc tttcccaatc
    35461 gggcgataat cgattgttcg gaatgtgggt tggtactttc cacagcattg ccaaccgctt
    35521 acttcgttcc cactatttag atgcggattt accgcaagat ttccaaatta tggataccga
    35581 agatcagcaa cgtttactca aacgtttact gaaactgcat aatattgatg agaaacattt
    35641 tccgccaaaa cacgttgcgt ggtatatcaa cgcccaaaaa gataaaggca aacgggcaaa
    35701 agatattgat cactataacg atccgaatga aaaaaaattg gtggagattt accaaatcta
    35761 tcaagatgcc tgcgaccgag ccggtttgtt agattttgcc gagctgttga ttcgggctta
    35821 tgaactattc aaaaccaaat caaccatttt gcaacgttat caacaacgtt ttcagcaaat
    35881 tttgattgat gagtttcaag acaccaacaa tattcagtac gacttaattc gtttgcttgc
    35941 cggcgatgtc ggcaaagtga tgattgtcgg cgatgatgat cagtctattt atggttggcg
    36001 tggggcacag gttgagaata tccaacgttt cttaaatgat tatgacaacg ctcaaaccat
    36061 tcgcttagag caaaattacc gctctaccgg caacatttta agctgtgcca atgaattaat
    36121 tgccaataac gataaccgct taggcaaaaa tttatggaca gatagcggtg atggcgagcc
    36181 tgtggatatt tactgtgcct tcaacgaact tgatgaagct cgctttgttg cctcgcaaat
    36241 caagcaatgg aaagaagacg agggtgattt ggcggaatgt gcggtgttgt atcgcagcaa
    36301 cagccaatct cgtgtgattg aggaagcttt aattcaagcg aatattcctt atcgcattta
    36361 cggtggaatg cgattctttg aacgccaaga aattaaagat gctctggctt atttacgctt
    36421 aatcgccaat cgtcaagacg atgcggcgtt tgaacgggtg atcaacacgc caaccagagg
    36481 cattggcgat cgtaccttag acacactccg tcaaattacc cgtggtcgcc aaattacgct
    36541 gtggcaagca attcaagtgg cggtgcaaga agaacaactt tcagggcgtg ccacttcttc
    36601 cttattgcgt tttgtagagc tgattaatgc tcttgatcaa gaaaccgagc agatgtcgct
    36661 tgccgaacaa accgattttg tgattaaaaa atcgggttta tatgaaatgt ataagcaaga
    36721 aaaaggcgaa aagggcgaag ttcggattga aaacttagaa gagttagtta ccgcaactaa
    36781 gcagtttgtg aaacctgacg aagcggaaga gctgaccgat cttaccgcat ttttaaccca
    36841 cgcctcactt gaggctggag aagcccaagc ctcgcaacac gaagattatg tcgaactgat
    36901 gacgctacat tccgccaaag gattggagtt tccccgtgtg tttattgtgg gcgtggaaga
    36961 agggattttc ccaagcggaa tgagctttga tgaaggcagg ttgcaagaag aacgccgttt
    37021 ggcttatgtc ggcataaccc gagctaagaa aaaactgacg attagctatg ccgaatcaag
    37081 acggctttat ggtaaagaag aacgccacgt tccgtctcgc tttattgctg aattacctga
    37141 agagcatatt cgagaagtga gattgagagg ctcaattaac cgagcggcaa gttttaacca
    37201 agctaattca tttgcaaaaa atccgacaaa aacgaccgct tcctttttaa acgatgatac
    37261 ttggaaaatg gggcagaaag tgaaccacgc taaatttgga caaggcacga ttattaatgt
    37321 ggagggctca ggtgaagcca cccgtttgca agttgccttt gtcggcaacg gcattaaatg
    37381 gctgattgcc aaaatggcaa atttagagag gatttaggcg aataaaaagt tttttgcagg
    37441 aatatagatg gcgagatata ttgcccctta accttaaact aagggtaaat aaaattaaat
    37501 aatgcaagct agatataatg taaagtatca gaaataagat attaatttaa gataaagaat
    37561 agatggctgg ggtactagga ttcgaaccta gggatgccga gatcaaaacc cggtgcctta
    37621 ccgcttggcg ataccccaaa agaaaaaact aacttacgtt agcttatata gatttgaatg
    37681 tttgaaatta agtggctggg gtactaggat tcgaacctag ggatgccgag atcaaaaccc
    37741 ggtgccttac cgcttggcga taccccaaca ctaatttttg ttaatcgata aatggtgcgg
    37801 gacgagggac ttgaacccac acacctcgcg gcgccagaac ctaaatctgg tgcgtctacc
    37861 aatttcgcca gtcccgcaat ggtggctaca gcgagattcg aacttgcgac cccaacatta
    37921 tgagtgttgt gctctaacca gctgagctat gtagccattt gcgttcacca tatcggtcaa
    37981 cggagtgcat tatgctgatt ttagggcttt ccgtcaaata ctttttttaa aaaatttgaa
    38041 tttttgattc gcttgtatag ttattcgtcg ttttggctta aatctagcat tttttgttta
    38101 taaaataata gcttatattc tgctatttca ccgtttgcat ttctaatttt aattgagcca
    38161 gtacaggctc aacatcgggg aattgccctt cccacaaaga aaacgcaaac gctgcctgtc
    38221 caaccaacat tcctaatccg tcttggcacg ctgttgctcc acaacgcctt gcataattga
    38281 gaaaaggagt ttgcatattg ggggcatatt gcatatcgta aatattcgcc gatgctataa
    38341 cagattcagg caattcaaca tatttgcctt gcagaccgag agaggtggca ttgacgatta
    38401 aattaaacag ttggtttgcc aaatcgctta aacaagcggt ctgaattttg ccaaattttg
    38461 caaatttttc ggctaaaacg accgctttct cctgcgtgcg gttatataga gtaatctcta
    38521 accccgcttt aagtaacgga agtaatacgc ctttggttgc tccacctgct ccaagaatta
    38581 aggttttatg ccctttttgt aaccaaccta gccgttccaa atcagaaact aaaccggccc
    38641 cgtcggtatt atcggcatat aaccgtccat cttctaagcg tttaagcgta ttacacgcct
    38701 gtgccaacaa agcggtttcg ctaaattcat ctgccaattt aaaggctctt tctttaaaag
    38761 gggcggtgat gttgcaacct tttgctccag aagcgaaaaa atcggctaat tgttgctcaa
    38821 aagccgtttc atctcctaag ttagcgatat attcaatgtt gcgttgggtt tgtaaagcaa
    38881 agagttgatg aatacggggg gatttacttt gagcaatcgg gttgccccac acggcatatc
    38941 ggttcatttc ggcttatcct tgtctaaaaa tctgttggct gaaaatatct cgaatttcgg
    39001 aagggttttc tctacctagc gtgtctgcgt gtaatacagg aaaatcttga ccaaactgat
    39061 cgatgacttc ttttgcattg cggcaaggtg gttctccgct caaattggca ctggtagagg
    39121 tcaacgcaac gccgcttgcc tcacacaact gaaccaccgc cggcacatta cataaacgta
    39181 cggcaataga atcaaatttg ccggtgaggt aacttggcac agtattttta accggtacga
    39241 tccaagtaat tgcctgctcg ccaatatggc ttaggcgttg ccaagcctgt tccgtcattc
    39301 gagcttcatc aatataggga agaagatact ttaaatcaga agccagtaaa attagccctt
    39361 tttcctcagg gcgttgtttt aatacgagta atttccttac cgctttttca ctttgtggat
    39421 tgcagcctaa gccaaaaacc gcttcggttg ggtaagctac aacttcttct tgctttaatt
    39481 gattaactat ttctaaaaaa ttattcattc ggttttaatt ctgtttggtg ctggcaactt
    39541 ttgttagcac aaatggtgaa ttgcttgccc ttaatttttt tgattgtagc gagttcacaa
    39601 ccacattttt cacacaagtg gttaatcggt cgagttgcta atgtaaattt gcaattaggg
    39661 aagcctatac aaccataaaa tattttgcct gaacgccctt ttctagcaat cagtttatgt
    39721 tgcttgcatt cagggcaatc aaactcttct tcaacctcag cttcttcgtg tacgatgaag
    39781 tggcattcag ggtaatggct acagccgata aaaataccat aattgccttg ttttaattgg
    39841 aggtttccgc cacattcagg gcaaagttca tctaattgct taataatatg accggcttga
    39901 tgtaatggct taatataatc acattcaggg taagctgaac aacccaaaaa taaccctttt
    39961 ttgccctgct taatttgcaa cggcgagcca cattgtgggc aagtttcggt ctgtttggtt
    40021 gatttgaaaa gtaccatatt gatttgctaa ataagcgtaa attcaatcac aaaaaacgca
    40081 ttgtcattcg gataaatctc ccgaataact tgtttcaatt cggctaagct catattttct
    40141 tgtttagcgt gttgctcgtt taattcatca aacactatag gtgttaccga tagcacttta
    40201 atattggcga aaaatctatc ggtttcatta gtaaaaaccg ctaattcttg gtttggttga
    40261 aaataagatt ctgacttgtc tcgaatcgta attgtttttt taccgcttaa aatatcggct
    40321 tcaaatcggc tgaaaaaggt aattttctct ctattcatat tcatcgctac tgtaatccag
    40381 agccattaaa tgactaaaat gatcgttata aaaaccacgg ctaaccataa atcgccacat
    40441 tttctgcttt gctttcagat cccatacaag cggtcgtttt ttctcaaaaa tttgctcagc
    40501 taattcaaac caatccactt cggcgttttc taattcttgg ttaattaacc aatcttttac
    40561 ccctttaagt tttagctctt gctttaaacg gcgaggccca atgccttgca ttgaacgata
    40621 acggataaaa gtggtacaaa agcgttcatc actttgccac tgattcgctt gagctttttc
    40681 aatcgctgca tcaatctccg tgagttcata ctctttttgc tttagttttt gccgaagctc
    40741 ttgctcgctg taatcacgct ttgaaagtaa atatagcaga taattaaccg ctgtgtattt
    40801 atttgtttta gtagtagatg ttgtcattta tcggttataa ataaaaatgc gattttcacg
    40861 ttgagcgaaa accgcataaa tttaattaga gttcttcttc actaaattca tcatctgcat
    40921 tgtctgcatt ttcagaatct ggagtagccg taaaagttgg gtttgaaatg agtagatttc
    40981 gaatatcttg ctcaatttta ctcgccactt cagggtgttc ttttaaccat ttgatggcat
    41041 tatttttacc ctgaccgatt ttctcccctt cataagagaa ccaagcccct gctttattaa
    41101 tgaagccgtg agattccgct aaaatgagta attcattcat acgggcaatg ccctcaccgt
    41161 acataatatc aaattggact tcacggaacg gtggagcaac cttgtttttc acaattttca
    41221 cttttgtttc gctaccgata acctcatcac cgtcttttac tacgcctgaa cgacgaatat
    41281 ctaaacgtac tgaagcatag aatttaaggg cattaccacc agttgtggtt tcagggttac
    41341 cgaacattac tccaattttc atacgaattt ggttaatgaa aatcaccaag cagttggtcg
    41401 ctttgatatt tgcggttaat ttacgcaatg cttgtgacat taaacgagct tgtaagccca
    41461 tatgggaatc acccatatcg ccttcaatct ctgcttttgg tgtaagtgcc gcaacagagt
    41521 ccacaataat tacatcaacc gcaccggaac gaactaacgc atcacaaatt tctaatgctt
    41581 gttcgccatt atccggttgt gaaattaata atccatctgt atctacccct aacttacggg
    41641 cgtaaatagg atcaagtgcg tgttcggcat caataaaggc acaagtttta ccattttttt
    41701 gggcttgagc caccacagat aaagttaatg tagttttacc tgaagactcc ggtccataaa
    41761 tttccacaat acgacccatt ggtaaaccac caatgcctaa tgctgagtct aaaccaagag
    41821 aaccggttga aactgcttca atatcaaggg cttgagtatc gcctaacttc ataattgagc
    41881 ctttgccaaa ttgcttttca atttgagcta atgcggctgc caacgctttt tctttttgtt
    41941 ccggatcaat ctgtttaacc ggagtgttct tttgactttt tttctctgcc atattctatc
    42001 ctttgtaaac ttttatctaa aagcaaccgc ttgttgctg
//
LOCUS       DANLRK010000002        36293 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.002, whole genome shotgun sequence.
ACCESSION   DANLRK010000002 DANLRK010000000
VERSION     DANLRK010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 36293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36293
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.002"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(<1..746)
                     /locus_tag="PXA33_000051"
     CDS             complement(<1..746)
                     /locus_tag="PXA33_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chain length determination protein"
                     /protein_id="HDL5119437.1"
                     /translation="MADSVKSHEQSSDEIDLIEIFRALWDKKWWIVITTFITTLLAGI
                     YAFTAKEQWTSKATVIAPKLTELGEYLNVRREYSRILRVDPVDPNALSASLFNNFNRL
                     AQSQDTKNEFFITSNVYKTLAEGKDEQGQRTVLSNLSNENTSFIIPDAKKDPNAIGRT
                     LSFSAETPMEAQDTLGKLVQFINSKAFAVELEDFLVDFQNVLTALQYEKSLIQEDLSV
                     NKSVQLENLNKAYETAQKAGIKEYSKAFNQS"
     gene            895..1236
                     /gene="erpA"
                     /locus_tag="PXA33_000052"
     CDS             895..1236
                     /gene="erpA"
                     /locus_tag="PXA33_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005706127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HDL5119438.1"
                     /translation="MSDIQIPLIFTDAAARKVKSLIEGEENPNLRLRVYITGGGCSGF
                     QYGFTFDDQVNEGDLTIENQSVGLVVDPMSLQYLIGGTVDYTEGLDGSRFVVNNPNAS
                     STCGCGSSFSI"
     gene            complement(1341..1664)
                     /gene="yidD"
                     /locus_tag="PXA33_000053"
     CDS             complement(1341..1664)
                     /gene="yidD"
                     /locus_tag="PXA33_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HDL5119439.1"
                     /translation="MVKTHSPEQKAGTTESINQPPRLSLLAKILLLPIYFYRYAISPL
                     IGPRCRFYPTCSTYAVEAIKIHGAIKGGYLAFKRILRCNPLSEGGEDPVPLCCKGNCK
                     HNDKK"
     gene            complement(1613..1987)
                     /gene="rnpA"
                     /locus_tag="PXA33_000054"
     CDS             complement(1613..1987)
                     /gene="rnpA"
                     /locus_tag="PXA33_000054"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HDL5119440.1"
                     /translation="MKKLNFSRELRLLAPVQFKAVFEEPLRASTPQMTLLARENNLEN
                     PRLGLTVAKKHLKRAHDRNRIKRIVRESFRLKQHELPNFDFVFVAKGGIGKLDNATLF
                     ETLEKLWSRHIRLSKKPAQQNP"
     gene            complement(2023..2157)
                     /gene="rpmH"
                     /locus_tag="PXA33_000055"
     CDS             complement(2023..2157)
                     /gene="rpmH"
                     /locus_tag="PXA33_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HDL5119441.1"
                     /translation="MKRTFQPSVLKRARTHGFRARMATKNGRQVLARRRAKGRKSLSA
                     "
     gene            2394..3356
                     /gene="hemH"
                     /locus_tag="PXA33_000056"
     CDS             2394..3356
                     /gene="hemH"
                     /locus_tag="PXA33_000056"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HDL5119442.1"
                     /translation="MNQPQQIGILLANLGTPDEPTPSAVKRYLKQFLSDPRVIDLPKF
                     KWQAILNCIILPRRSPKVAKLYQSVWSEEGSPLLAISRQQQRAVQRYFDAKGKNVVVE
                     LGMSYGNPSIESATDNLIKQGVERIIVLPLYPQYSSTTTASVFDAFANGLKAQRKIVP
                     FDFIHSYHNDPLYIQALANSIDVKPDELLLFSFHGIPKRYETEGDFYAKHCQETAELV
                     AEKLGLPREKWYLSFQSRFGDEEWLQPYTDQTLESFPQKGIKKVAVVCPGFSADCLET
                     LEEITEENRENFEHAGGESYRYIPALNASGDHIKALVKLIEAKI"
     gene            complement(3420..4367)
                     /locus_tag="PXA33_000057"
     CDS             complement(3420..4367)
                     /locus_tag="PXA33_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HDL5119443.1"
                     /translation="MPTKPHKEYHELVELLISRGMLINDKSYAEKKLSQVGYYRLSGF
                     WHISRKPNQDGGLSENFLENVQFEEVYKLYIFDKKLRLLLLDIIERLEVNIRNIMAHE
                     LGRENPLAYEDYSLINQKFLKFQWKSNYAYANWLETTANKIKRSKDDSIEWHRKNEKE
                     IPFWVITETWDFGALSKFYSLLNGQHQRKIAKKFNVDENTLKNWLNEINIVRNLSAHH
                     SRIWNRKFSDIAIPKLDDPRWSKFKKMQQYFSNLSLDDKAKERIFGRVAILWFLVSQT
                     STNYHWLDKFEDLLNDFPNLPNTKVELMGFNREILEKIK"
     gene            complement(4579..6696)
                     /gene="spoT"
                     /locus_tag="PXA33_000058"
     CDS             complement(4579..6696)
                     /gene="spoT"
                     /locus_tag="PXA33_000058"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HDL5119444.1"
                     /translation="MYLFEPLDRIIQGYLPPEQIERVKRAYVIARDAHEGQTRSSGEP
                     YITHPVAVASIIAEMKLDQEAVIAALLHDVIEDTPYTEEELAAEFGQSVADIVQGVSK
                     LDKLKFRTRQEAEVANFRKMILAMTKDIRVVLIKLADRTHNMRTLGALRPDKRRRIAK
                     ETLEIYSPLAHRLGIEHIKNELEDLGFEAMHPQRYRVLKMAIASARGHRKELIQRIAD
                     DIRGRLEEVQIEARVYGREKHLYSIYQKMRQKDQHFHSILDIYAFRVVVDNVDNCYRV
                     LGQMHALYKPRPKRVKDYIAVPKPNGYQSLHTSMIGPHGVPVEVQIRTEDMDKMADMG
                     VTAHWRYKEGESHHNTTVQLKAQQWLQSIVELQNSAGNTAEFIDSVKSDLFSDDIFVF
                     TPKGRIVELPANATAIDFAYAVHSGIGDRCVGAMVDRNPYPLSEPLRTGQTVEIITEQ
                     GKRPNASWLNFVVSGKARAKIRQSLKNQQHDEAIALGRRQLVRAMNLRSLDELCSETI
                     QSVLDELRLSSFDELLAEIGLGNQMSNVIAQRLLGEPLLIDTDGNPENNQPLKIEGIE
                     NRLISFANCCLPIPGDEIIAHVSSGKGLAIHNACCGNVKDYATEPSNYLPVEWEIAKE
                     KAIQFETEIVLDIINEPGVLAGITSTLAKLNSNIGSINSEAREGNIYQVRVQISVENK
                     EHSELILRKLLSVKGVVKAARKQ"
     gene            complement(6806..7087)
                     /gene="rpoZ"
                     /locus_tag="PXA33_000059"
     CDS             complement(6806..7087)
                     /gene="rpoZ"
                     /locus_tag="PXA33_000059"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005613378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDL5119445.1"
                     /translation="MARVTVQEAAEKIGNRFDLILIAARRARQLQLHTREPLVPEEND
                     KPTVIALREIEEGLITTDIMDQLENHDSIKQEAAEQEAISFLTEMSNEA"
     gene            complement(7190..7687)
                     /locus_tag="PXA33_000060"
     CDS             complement(7190..7687)
                     /locus_tag="PXA33_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDL5119446.1"
                     /translation="MAHFYEYLEFNSDEDRENQLEVYVEVKLSTDTEEKLQAVGVQGD
                     WLVMAEPYCPDCVEIVAYFQRMAKLNPNIKVKYVARKDNQERKHFDSDEQQQAVISAQ
                     KIPTIFDIRNGKTELVLSEFPQFLKDKMAANPEQFEQLKADFRMGKFGKEVEAELLAI
                     LLNTK"
     gene            complement(7712..8734)
                     /locus_tag="PXA33_000061"
     CDS             complement(7712..8734)
                     /locus_tag="PXA33_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005825060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL5119447.1"
                     /translation="MSVTKKRLTLSDIAELSGVSKTTVSMILNGRGNDFRIKSETCQK
                     VKEIAKKHGYRANVYAKSLQAQRTNVIGLVIPDLTNYGFALTSRTLEKLCRENGLQLV
                     IACSDDTFEGEKKAIERLLDRQVDLLITAPTHNEPTYYQNAAQYTPMLQLDRYISGLN
                     IPSITSNDTQSISMLVENIVRAYHLKEFFYLGGQLSLSPSQNRLKGFREGLEQSHLAL
                     DENWVLHQDYQPESGYQMFAQIVEKLGRLPEAVFTASFTLLEGVMRYLTEHQQMDKLL
                     TQELHLATFDDHHLLNALPFHIHSIKQDHQQIAERTFELIQQKLAQQKIDDVKVDCEI
                     LWRKSI"
     gene            complement(8774..10204)
                     /locus_tag="PXA33_000062"
     CDS             complement(8774..10204)
                     /locus_tag="PXA33_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HDL5119448.1"
                     /translation="MHIFNNGKYKSLHAQSEGELNALRQTVLSDPDFYPTYHLAPETG
                     LFNDPNGLIFDGEKYHIFAQWFPYGALHGMKHWQHFITTDFQTFSKGDLLIPDELFES
                     HGCYSGGAILWQDKIVAFYTGNTRRPSDNARIPHQNIAIFDKSGKLLEKRCIIDQAPA
                     GYTEHVRDPKPYLTAEGKIRFVLGAQRENLTGTALIYEMDDLNATPRLIAELEVHNFD
                     NSNVFMWECPDLFSLGGKDLFVWSPQGKLRESHQFQNNYHATYALGELNGNRLRAEHI
                     EELDYGFDFYAPQTVENSDRILFGWVGLPDLTYPTDKYQWHSMLTLPRKLSLQNGKVI
                     QNPIVNVGELQAVEMSENFAIENLDTAYLQISVENQPLVLDFFSNEQGQKLSLRFENG
                     VLSLDRSQTEQTELMEKFGSVRHCKIEQLETIEIFFDRSVVEIFVNGGEKVLTSRFFI
                     AKRENVVKSSRPIQVKVAKVLPISVE"
     gene            complement(10309..11232)
                     /locus_tag="PXA33_000063"
     CDS             complement(10309..11232)
                     /locus_tag="PXA33_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005606644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HDL5119449.1"
                     /translation="MSKIWVTGDAVVDLIPDGEHHYLKCAGGAPANVAVGVSRLGVEA
                     GFIGRVGNDPLGKFMREVLQAENVCTKQMILDAQHRTSTVIVGLDNGERSFTFMVNPS
                     ADQFLEIGDLPEFNQGDFLHCCSIALINDPSRSTTIEGIRRVKKAGGYVSFDPNLRES
                     LWKSLDEMKTVVNSVVAMADILKFSEEELTLLTETETLEQATQAITAQYPEKLIIITL
                     GKDGAIYHFNGKSQVVAGKALKPVDTTGAGDAFVSGLLAGLAQTPNWHDETVLVEVIR
                     KANASGALATTAKGAMSALPNKAELDAFLAE"
     gene            complement(11349..12776)
                     /gene="miaB"
                     /locus_tag="PXA33_000064"
     CDS             complement(11349..12776)
                     /gene="miaB"
                     /locus_tag="PXA33_000064"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HDL5119450.1"
                     /translation="MAKLHITTWGCQMNEYDSSKMADLLNSTHGFELTENPEDADVLL
                     LNTCSIREKAQEKVFSQLGRWKNWKKDKPNLIIGVGGCVASQEGEHIRDRAPYVDIVF
                     GPQTLHRLPEMINQIRGGKSSIVDISFPEIEKFDRLPEPKAEGPTAFVSIMEGCNKYC
                     SFCVVPYTRGEEVSRPVDDVLFEIAQLAAQGVREVNLLGQNVNAYRGETFDGGICTFA
                     ELLRLVAAIDGIDRLRYTTSHPIEFTDDIIEVYRDTPELVSFLHLPIQSGSDRVLTMM
                     KRNHTALEYKAIIRKLRAVRPEIQISSDFIVGFPGETAEDFEQTMKVIEQVNFDMSFS
                     FIYSARPGTPAADLPDNVSDDEKKERLSRLQQRINHQAMQFSRAMLGTEQRILVEGPS
                     KKDLMELTGRTETNRVVNFVGTPDMIGKFVDVKITDVYTNSLRGEVIRTEDQMGLRVV
                     ESAESVIARTRKEDDLGVGKYVVNL"
     gene            complement(12993..13493)
                     /gene="crr"
                     /locus_tag="PXA33_000065"
     CDS             complement(12993..13493)
                     /gene="crr"
                     /locus_tag="PXA33_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005702996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL5119451.1"
                     /translation="MGLFDKLFGSKQKESQEIKIYAPLTGEIVNIEDVPDVVFSEKIV
                     GDGVAIRPSGDTIVAPVNGTIGKIFETNHAFSIESEEGVELFVHFGIDTVELKGEGFT
                     RLAQEGQTVKVGEPIIKFDLALLEGKAKSVLTPIVISNMDEISNLSKLNGQVVAGESV
                     VLTLTK"
     gene            complement(13578..15299)
                     /gene="ptsI"
                     /locus_tag="PXA33_000066"
     CDS             complement(13578..15299)
                     /gene="ptsI"
                     /locus_tag="PXA33_000066"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HDL5119452.1"
                     /translation="MITGIAASPGAVFGKALVLKEEPIVLNTRKITESEVEAEKAKFF
                     TGREKAAAQLTAIKEKARRTLGEEKEAIFEGHLMILEDEELEEEILSYLTDNLVTADV
                     AASKVIEMQASMLAEIDDEYLKERAGDIRDIGNRLVRNILNMNIVDLGDIQDEVILVA
                     YDLTPSETAQLNLDKVLGFITDVGGRTSHTSIMARSLELPAIVGTNNVTSLVKTGDFL
                     ILDAVNNEVHLNPSEEKIANFKALQEKLNAEKEELAKLKELPAQTLDGHKVDVAGNIG
                     TIRDVEGVLRNGGEAVGLYRTEFLFMDRTQLPGEEEQFQAYKEIVEAMNGQMVILRTM
                     DIGGDKELPYLDLPKEMNPFLGWRAVRIGFTRREILDTQLRAVLRASAFGNLAVMFPM
                     IISVEEIRLLKSIVAELKDQLRAEGHAFDENLKLGIMVETPSAAVNARHLAKEADFFS
                     IGTNDLTQYTLAVDRGNEIIAHLYNPLSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     VRATALLLGMGLDEFSMSGISVPHVKKLIRSINFADAKALADEALAQPTAADIERLVD
                     EFYQKLN"
     gene            complement(15421..15678)
                     /gene="ptsH"
                     /locus_tag="PXA33_000067"
     CDS             complement(15421..15678)
                     /gene="ptsH"
                     /locus_tag="PXA33_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HDL5119453.1"
                     /translation="MYSKDVVITAPNGLHTRPAAEFVKAAKGFASDITVTSGGKSASA
                     KSLFKLQTLGLTQGTTITISADGEDEQKAVDFLVDLIPTLE"
     gene            15939..17513
                     /gene="thiP"
                     /locus_tag="PXA33_000068"
     CDS             15939..17513
                     /gene="thiP"
                     /locus_tag="PXA33_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease"
                     /protein_id="HDL5119454.1"
                     /translation="MLSTLTKTTAWFIYFSIIALYVFSLWALMDYHQNDTLFVFSDIT
                     ATVAYSLLQASLSAVISLFLGSLLARSFFYLDFKGKNLLYKTISFIWALPSLVIIFAV
                     IGVFGNSGWLAKLFQQLGWNWQFNLYGLHGIILAHCLFNIPFVMKYYTGGLQLTPSSR
                     FQLAAQLNLTGWNYIQIVELPIIKTLLPYMFMTVFLVCFTSFPIVLMLGGSPKYSTLE
                     VAIYQAVTFEFDFAKALILIGVQFVVGIGLQLVMNYISTSALKKLKQQPNRVEIWKPK
                     PTGLAKLGLQAVIFLQSFAIILPLVSVIWAGLSVSKLSERLLNPTLWQATQYSLYLSL
                     IASIFVITISYLIALEARQLLYTKAKLRYSILTAITLFPLLLPIFLLSVGLFILLMNS
                     ELSSEALLLLVGICNGLTLLPFIYPMLFSAMWNSLTSHDKLAQGLGLKGLRRWWIVEK
                     NQLIRPLVTVFALAMSSSLGSFTVIAFFGNADFSSLPYLLYQQLGSYRTEDAAVTALI
                     LMLFALLPFLFSKDKL"
     gene            17510..18160
                     /gene="thiQ"
                     /locus_tag="PXA33_000069"
     CDS             17510..18160
                     /gene="thiQ"
                     /locus_tag="PXA33_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein"
                     /protein_id="HDL5119455.1"
                     /translation="MIELDLHFTYEQMNMHFQLSVKKGERIAVVGESGAGKSTLLNLI
                     AGFEKAESQQSQLWLNQKNHLDTEVAQRPVSMLFQDNNIFPHLTVEQNIGLALVPSLS
                     LNPKQKAQVKQIATQMGIETFLQRQADQLSGGQKQRVALARTLLRDKPILLLDEPFSA
                     LDPQRRAELQQIVFDICQSRNLTLLMVTHQLSESLFLFNRVITVADGEIVSDLSPL"
     gene            18467..19881
                     /locus_tag="PXA33_000070"
                     /pseudo
     CDS             18467..19881
                     /locus_tag="PXA33_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605131.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sucrose transporter subunit IIBC"
     gene            complement(19997..20800)
                     /gene="map"
                     /locus_tag="PXA33_000071"
     CDS             complement(19997..20800)
                     /gene="map"
                     /locus_tag="PXA33_000071"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HDL5119456.1"
                     /translation="MNNIPLRNEEEIQKLRVACKLASDVLVMIAPYVKEGVTTGELDK
                     ICHEYILANGATSASLGYHGFPKSVCISLNEVVCHGIPSDDKKLKKGDILNIDVTVIK
                     DGYYGDNSMMYVVGEPSVRDKRLMAVTLESLYVGIRTVKAGVRLKEIGTAIQKYVEKE
                     GFSVVREYCGHGIGTEFHCDPQVVHYATDDGGVVLKEGMVFTIEPMVNAGKKEVRLMG
                     DGWTVKTKDRSHSAQYEHQIVVTKDGCEVLTIRDEEIAEGRISRIMHNS"
     gene            complement(20886..21551)
                     /locus_tag="PXA33_000072"
     CDS             complement(20886..21551)
                     /locus_tag="PXA33_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HDL5119457.1"
                     /translation="MNFTTYFSDENKRRSLILLSLFHIFIIAISNYLVQISFEVQVPL
                     TEFSFPTTWGTLTFPFIFLATDLTVRVFGADLARKIIFVVMFPALVISYVISVLFFET
                     QFQGFSALTEFNFFVFRIALASFAAYLVGQLLDVLVFNSLRQGKRWWLAPTCSTIFGT
                     LIDTFVFFAIAFYKSSDEYMAEHWFTIGSVDYAFKLFVSLLLFLPLYGVILNFIMKKL
                     DLK"
     gene            21744..22433
                     /locus_tag="PXA33_000073"
     CDS             21744..22433
                     /locus_tag="PXA33_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HDL5119458.1"
                     /translation="MLQLRKANERGDGSFSWLKSYHTFSFANYYDLKHIHFSHLRVIN
                     EDFIAPNHGFGKHPHDNMEILTYMLKGRVAHEDSMGNKTEVKAGEFQIMSAGTGVFHS
                     EINPDPNETLHLYQIWIIPNQKDVTPRYNQDRFAPKEGATLILSPNAEEGSFKIYQDM
                     KLWRYQYPTAKSEQLALEPNRSYWLQVVKGNLNINGVELSTSDALGIRQEKALEINTQ
                     DDVEFLLFDLV"
     gene            complement(22501..23292)
                     /locus_tag="PXA33_000074"
     CDS             complement(22501..23292)
                     /locus_tag="PXA33_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HDL5119459.1"
                     /translation="MNNLRIKMISPEIALAKYNELDVLEINHSKVKAKIALQGAQLLS
                     WQPKNTQQDVLWLSEIEPFQTGVAIRGGIPICHPWFGTDKKPAHGTARIQAWNLLEHT
                     SSADGIRVVLGLENQAKLEMILGEVCELYFTHLAPEPAQAALHTYFQVGDITKTEVQG
                     LATQCFDKLTNQEIEVPSPRKISENVDCIYPAQAVNFIQDFANQRTIKVEHINATETV
                     LWNPWHNTVSAMTETAYQNMVCVETARINTLLQQNETLGVRILVR"
     gene            23416..24420
                     /gene="fbp"
                     /locus_tag="PXA33_000075"
     CDS             23416..24420
                     /gene="fbp"
                     /locus_tag="PXA33_000075"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HDL5119460.1"
                     /translation="MKTLGEFIIEKQAEYPGAKGELSGILSSIRLAAKIIHREINRAG
                     LSQDILGTAGSENVQGEAQMKLDVFANETMKKALLAREDVAGFASEEDDNFVAFDNER
                     GRNAKYILMTDPLDGSSNIEVNVSVGTIFSIYRRVSPIGTPVTQEDFLQEGRKQVAAG
                     YVTYGSSTMLVYTTGNGVNGFTYDPSLGLFILSHPDLKTPTEGKYYSINEGQYVTFPQ
                     GVKRFIKYCQETDEAIKRPYSSRYIGSLVSDFHRNLLKGGIYIYPTSTVYPKGKLRLL
                     YECNPIAFLAEQAGGMATDGINPILDIKPTELHQRVPFFVGSTSMVKQAGEFMQEFAK
                     "
     gene            24604..29115
                     /locus_tag="PXA33_000076"
     CDS             24604..29115
                     /locus_tag="PXA33_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HDL5119461.1"
                     /translation="MINKNFPLSYLASFVAFAVSLSATASVFRDDIDLQYYRDFAENK
                     GKFTVGASNIEIKDKSGNTLDALFPNVPMPDFSAANRNLGIATLVAPQYLVSVAHNTQ
                     YNTVEFGAPGTNADAHHYTYKVVDRNDYGIVEGGQHQDYQVPRLNKLVTEVAPATVTD
                     LGNNASAYQDSSRFTHFARLGSGRQIVKNTEHKDNQISTSYQYLTGGVHLPIAVHNSD
                     YWLDFRGNALNNSPYGALTAFGTRGDSGSGVYGYDQKTKRWLLLATYTFGTPANNYYN
                     RAGIIRQDYHDKQFAEDIAGTLTNAQQNAVFEWSAQGKDSSIGNKGKNLTVSLADRSI
                     KDNSASPLGYGGTPQLQLPQDNTGKTLNIEGQDSTIVLKTDIDQGAGALNFNANATVR
                     PENDQSWVGAGIVVAKGKQVNWQVKNPQGDRLSKLGEGTLHINGKGENLGDISVGQGT
                     VILTQQAGENGKKSAFNQVGIVSGRSTVVLNSADQVDPNKIYFGFRGGRLDLNGNNIA
                     FNRIQNSDDGARIVNNHLQKAATLTINGPKPPEATDLKWGTWKENSADIYEYINPHAN
                     NRTDYFTLKGNPNQYMPTNGASNAHWTFLSSNKDAAVKQVLAQKGLEHRYNSFNGFIG
                     ETDNTQHNGRLNVVYDPKASTPPATASEVTWGKGLVAGADIYLFTNPKTKIREYFALK
                     GDPKQPVPRGGLSSEHWEFLAADRNQAINKVLARKNAPIEQEKLNSIYTFSGGLNLNG
                     DLTVKGGKVLLSGRPTPHAYDAINKQDVVYADDWQNRQFKADNMQLNQYAQLYVGRNV
                     SNLQANLSANDHAQLHLGFINEQTPSCYYSEYTGKTSCDTQAVVSNEIFATLPTTQIN
                     GDVSLAEQSQLHIGKANLIGTIQAAATTSIRLANQASWTNTGDSRTGNLVAENGSTIN
                     LNEKFVTGEIPTRFNTLIIDGNFQGNAKINYLTDIAAGKGDHLQVNGLAEGTFTLALR
                     NSGKEAEVVSPLSLLTLTHQAQADKAKVSLENGYVDLGAYRYVLANRSNGYRLYNPLK
                     DAKDRNQSIATAKAELDHAIAEADKQKQEISRLNAEAEKERQAEKNAKQAAANAQSQL
                     SQANSELTRLQQYADYYRRYYPTYYRQIQGQITVAKQKVTQASAALTTAENNAKASAA
                     QIANVEQAVETAQNIAKQVEAKLATLRITAGNSEAILNAEALKLCQENGANCEHLQAH
                     ADTDSSNIQQSDWVSQYANTALSELSAQANSALQIGQDLDRQLFAKHDKFHVWSSIEH
                     QKTEHQSDLYRPYEQQTNLTQLGIELPLANGINAGVMLSRNHANAEFDEGVNGKSNLL
                     MASLYGKWHSENGTFVSLDGSYGKAKNRIDLFGENRFNRHIMAIGANLGHNFDLAGVQ
                     VQPAVGTRYYRFSAQNYKLGEVEVRSPKAHFMAYQAGVKVSKAFDLSSWKVEPSLAAH
                     YVDASSKRLSVAVNDNTFSQRFGRYLKTEVGVGVTKGQWQLSSHLGLLKGNEIGKQHF
                     AGFKLGYSW"
     gene            complement(29170..30279)
                     /gene="mobA"
                     /locus_tag="PXA33_000077"
     CDS             complement(29170..30279)
                     /gene="mobA"
                     /locus_tag="PXA33_000077"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HDL5119462.1"
                     /translation="MEEKISAVILAGGLARRMNGAEKGLQLLDQKPLISHLLARLSPQ
                     IEDIWLNINRSVEQYQQLYPELPYYQDSLPDFQGPLSGMLSGFEQIESDYLLFVPCDT
                     PFIPTQLLHKLHTALRINQAQIAYAHDGDRPHPTVALVHRSVAENLRAYLASGERRLL
                     WFFQTQKSVAVDFSAYKAAFRNFNCLEDLTAFNPYPIKTLAITGYSGTGKTTLLEKLI
                     PKLTACGIRVGLIKHSHHNVDIDKKGKDSYRLREAGANPTMVVCDERWALMTETKQAA
                     ETKQAVEFSQLIAKFDPETVDLVLVEGFKHETMPKIQLHRKEIEKPLPALDQWTIATA
                     TDYPLERENRLDINDVEQLADFIRQWLEASKEPAN"
     gene            30371..30640
                     /locus_tag="PXA33_000078"
     CDS             30371..30640
                     /locus_tag="PXA33_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HDL5119463.1"
                     /translation="MKCKRLEELLELLGEHWRKNPDLHLIDILQQLSVEVSEPDNFHA
                     LRDEVLIYQLKMRNADKFEPIPGIKKDYEDDFKTALLRARGILND"
     gene            30684..31322
                     /locus_tag="PXA33_000079"
     CDS             30684..31322
                     /locus_tag="PXA33_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HDL5119464.1"
                     /translation="MKKFALKTVLVALSALFAFNATAVAADPTAGKEYIEVRKAPSAQ
                     KEVVEFFSFYCPHCYDFELNYKIPSQIKAKLPADSKLVQYHVNFLGRQSENLTRAWAL
                     AMALGAEDKVKTALFEAAQKDAFKSMDDIRAVFIANSISAEQFDSGINSFAVNGLVNK
                     QVQLAEDFQIRGVPAFFVNGQYQLNLEGFSDSSSTNDFIQRYIDAVVFLSKK"
     gene            31342..31794
                     /locus_tag="PXA33_000080"
     CDS             31342..31794
                     /locus_tag="PXA33_000080"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MsnO8 family LLM class oxidoreductase"
                     /protein_id="HDL5119465.1"
                     /translation="MVLQVLNSKAIQPFLYANSENKMTNLSILNLVPIREGQTAKQAV
                     DSMVKLAKHAENIGIERYWIAEHHNMKNLASSATQLLIQHALSHTEKLRVGSGGVMLP
                     NHSPYIVAEQYGTLETLYPNRVELGLGRAPGTDMRTASALRPEFAFPR"
     gene            31778..32440
                     /locus_tag="PXA33_000081"
     CDS             31778..32440
                     /locus_tag="PXA33_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HDL5119466.1"
                     /translation="MPFPDEISELKGYFDGTNPVSAYPAQGLNVPFYILGSSPESAYL
                     AAERGLPYAFASHFAPRFMDDAIRIYRSHFKPSDVLDKPYVILGVNAIVAETDEEATQ
                     LATTQTQFFLNVVTNAQQNLQPPLESDDKVWENFVKAEIVPHFGPVAFEQNALYRQEK
                     TVVRQMTECSLIGSPQSVEQQLKALKARVEIDEIMAVNYIFDEQKQHQSYTWLKQITD
                     KV"
     gene            32442..32735
                     /locus_tag="PXA33_000082"
     CDS             32442..32735
                     /locus_tag="PXA33_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HDL5119467.1"
                     /translation="MSKTMQQKAAEAHNMCRLKGDSMLDNADRQIAFEAVYESEELAQ
                     QAVEFFAQKAKSVETEPCEIQSEITQIAEGFLMKMWITFSCQAEVILFQMAVR"
     gene            complement(32804..34138)
                     /gene="srmB"
                     /locus_tag="PXA33_000083"
     CDS             complement(32804..34138)
                     /gene="srmB"
                     /locus_tag="PXA33_000083"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HDL5119468.1"
                     /translation="MTQLMTFEELDLSPQLLKALAHKGYNRPTAIQQATIPAALEGRD
                     LLGSAPTGTGKTAAFLLPAIQHLLDYPRRKPGAPRILILTPTRELAMQVAEQAQALAK
                     FTQLSIATITGGVAYQNHGEVFNKNQDIVVATPGRLMQYIKEENFDCRAVEVLIFDEA
                     DRMLQMGFGQDAEKIAAETRWRKHTWLFSATLEGELLEDFAERLLNDPIKVDAEPSRR
                     ERKKIQQWYYHADNVEHKTKLLARIISEFSVERAIVFVRRREDVRELSETLRKRGIRS
                     TYLEGEMAQTQRNQAINRLKEGVVNVLVATDVAARGIDIDDIDFVLNYDLPYSADTYL
                     HRIGRTARAGKKGSAISLVEAHDYKLLGKIKRYTEEVLKPRIIEGLEPRTKAPKDGEL
                     NVTSKKEKARIKKKKEAKKEAAKPKVKVRHKDTKNIGKRRKPSSESAAVKQA"
     gene            34298..34561
                     /locus_tag="PXA33_000084"
     CDS             34298..34561
                     /locus_tag="PXA33_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrxA family glutaredoxin"
                     /protein_id="HDL5119469.1"
                     /translation="MFVEIYGRLSCPYCVRAKTLAEKMKQELSDFDFKFIDMIAENIS
                     KQDLEPRVGKPVATVPQIFVDNNHVGGCTDFQAFVEEKFGITV"
     gene            complement(34609..34707)
                     /locus_tag="PXA33_000085"
     CDS             complement(34609..34707)
                     /locus_tag="PXA33_000085"
                     /inference="COORDINATES: protein motif:HMM:NF028261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine/alanine import family NSS transporter
                     small subunit"
                     /protein_id="HDL5119470.1"
                     /translation="MSSAAIITMVVAMTIIWGGLLFAIKRLPKEEQ"
     gene            complement(34707..36215)
                     /locus_tag="PXA33_000086"
     CDS             complement(34707..36215)
                     /locus_tag="PXA33_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HDL5119471.1"
                     /translation="MSNQSSNAPQRETFSGRGAFIMAAIGSAVGLGNIWRFPYTTYEN
                     GGGAFILPYIIALLTAGLPLLFLDYSIGHKYRGGAPLAFRRFSSKFETFGWWQVLINV
                     IIGIYYAVVLGWAASYTYFSFSGAWGDKPVDFFIGEFLKMGDITQGVSFEFVGMVTGP
                     LIAVWLIAIAVLAMGIKKGISAASNLLMPQLIVMFLVLVGYSLTLPGAAKGLDALFTP
                     NWEKLADPSVWIAAYGQIFFSLSICFGIMITYASYLKKETDLTGSGLVVGFANSGFEL
                     LAGIGVFAALGFMATAAGQEVNEVAKGGIGLAFFAFPTIINQAPMGALVGVLFFGSLT
                     FAALTSFISVIEVIISAVQDKLNSGRVKATLLVGIPMMVVSVLLFGTTTGLPMLDVMD
                     KFVNQFGIVAVAFVSLIAIVTSEKLSTLEKHLNEVSSFKVGFIWRLCIVITSGVLAFM
                     LFSEIAKVYREGYEGYPSWFVNTFGWGMAIGLVVVAYLLSKMAWKNEPKGDE"
BASE COUNT        10714 a         7919 c         7033 g        10627 t
ORIGIN      
        1 gattgattaa atgcttttga atattcttta atacctgctt tttgtgcagt ttcataggct
       61 ttatttaagt tttccaactg aaccgactta ttcacagata aatcttcctg aattaatgat
      121 ttttcatatt gtaatgcagt aagcacattt tggaaatcga ctagaaaatc ttctaattca
      181 acagcaaatg ctttgctatt aatgaattga accaacttac caagggtgtc ctgcgcctcc
      241 ataggtgtct ctgcagaaaa agatagtgta cgacctattg cattaggatc tttctttgca
      301 tcaggaataa taaagctggt attttcatta ctcaaattgg ataaaactgt gcgttgcccc
      361 tgctcatctt ttccttcagc taacgtctta tacacattgg aagtgataaa aaattcattt
      421 ttagtatctt gagattgtgc taatcggtta aaattgttga acaaactcgc agacaatgca
      481 tttggatcaa caggatctac acgcaaaatc cgactatatt ctctgcgaac atttaaatac
      541 tcacctaact ctgttaattt aggggcaata actgttgcct tagacgtcca ttgctctttt
      601 gcagtaaatg cataaatacc agcaagtaat gtggtaatga atgttgttat aacaatccac
      661 cattttttat cccatagagc acgaaaaatc tcaattaaat caatctcatc cgagctttgc
      721 tcatgacttt ttactgaatc tgccatagtt tcctctaaaa tttgcatgtt tctgtacagc
      781 attatacata attttcttat ttccactgca ttcatttact cttttttaag attgcacttc
      841 ctatgtaatt attagataat atccaatcac agtaaaattt tgatctaaaa caaaatgagc
      901 gatattcaaa ttcctttaat ctttactgat gcggcggctc gtaaagtaaa aagcttaatt
      961 gagggcgaag agaacccgaa tcttcgcttg cgtgtgtata ttacgggtgg gggctgtagt
     1021 ggattccagt atgggttcac ctttgatgat caagtcaatg agggagacct taccattgag
     1081 aatcaaagcg ttggcttggt ggttgacccg atgagcctac aatatttaat tggtggcacg
     1141 gtggattata ccgaagggct ggacggctca cgttttgtcg tgaataatcc aaatgcaagc
     1201 tcaacctgtg gttgtggttc ttcctttagc atttaattca ctcgaattat tgccttattc
     1261 aatatcagtc tcacaagtaa attttttact aaaaatgacc gtttgtatta aacaaaatag
     1321 cctaacttat tgagtttagg ctatttttta tcattatgtt tgcagttgcc tttacaacaa
     1381 agtgggacag gatcttcgcc tccctcactc aaagggttgc aacgcagaat acgcttaaac
     1441 gctaaatacc cacctttaat tgctccgtgg attttaatcg cttcgaccgc ataggtggag
     1501 caggttgggt aaaatcggca acgtgggcca atcaatgggc tgatagcata gcgatagaaa
     1561 taaatcggta gcaatagaat tttggctaat aggctgagac gaggtggctg atttatggat
     1621 tctgttgtgc cggctttttg ctcaggcgaa tgtgtcttga ccatagtttt tccaatgttt
     1681 caaacagcgt tgcgttgtct aatttgccaa tgccgccttt tgccacaaat acaaaatcga
     1741 aattcggcaa ttcgtgttgc ttgaggcgaa aactttcacg cactatccgc ttaatgcgat
     1801 tacggtcgtg agcccgtttt aaatgttttt tggcaacagt taagcctaga cgaggatttt
     1861 ctagattatt ctctctagca agaagagtca tttggggtgt gctggctcga agtggctctt
     1921 caaacaccgc cttaaattga acgggagcta acaaacgtag ctcccgagag aaatttagct
     1981 tcttcacact taatcagaaa ggtgttttgg ctggttctgc aattatgcag ataaagattt
     2041 acgaccttta gcacgacgac gcgctaacac ttgacgaccg tttttagtag ccatacgagc
     2101 acggaaaccg tgagtacgag cacgttttaa tactgatggt tgaaatgtac gtttcattgc
     2161 ttatctacct aaaatttaaa aagttggcat aaaatggttt ctgccaaaca aggaccataa
     2221 aaaagagttg caattctaca ggcaattaaa ataaaaagca agaaacaatc caagcacaac
     2281 tcgggttgtt tgcaaaattt ttgaaaaata cgaccgcttg ttataatcgc agaaatttat
     2341 attggtttgt ggcataatcg aaaaaatatt aactattgat gagaaataga acaatgaatc
     2401 aaccccaaca aataggtatt ttgctggcta atttaggtac gccagatgaa cccacgccaa
     2461 gtgcggtgaa gcgttattta aagcaatttt taagcgatcc tcgtgtgatc gatctaccaa
     2521 aatttaaatg gcaagcaatc ctaaactgca tcattttgcc aagacgttcg ccaaaagtgg
     2581 caaaattata tcaatcagta tggagcgaag aaggctcacc gttattggcg atttctcggc
     2641 aacaacaacg agcggtacaa cgctattttg atgccaaagg caaaaatgtg gtggtggaat
     2701 tgggaatgtc ttacggcaat ccaagtattg aaagtgcgac cgataacctc atcaagcagg
     2761 gggttgagcg gattattgtg ctaccgcttt atccgcaata cagtagcact actaccgctt
     2821 cggtgtttga tgcgtttgcc aacggtttaa aagcacagcg taaaatcgtg ccgtttgatt
     2881 ttattcacag ctatcataac gatccgcttt atattcaagc gttggctaac tctattgatg
     2941 ttaaacctga cgagttatta ctgttttcat ttcacggcat tcctaaacgt tatgaaacgg
     3001 aaggtgattt ttacgccaag cattgccaag aaacagcaga attagtggct gaaaagctag
     3061 gtctgccaag agaaaaatgg tatttgtctt tccagtcccg ttttggtgat gaagagtggc
     3121 tacagcctta taccgatcaa acccttgaaa gcttcccaca aaaagggata aaaaaagtgg
     3181 ctgtcgtttg cccgggcttt tcagcagatt gtttggaaac cttagaagaa attacagaag
     3241 aaaaccgcga gaactttgaa catgccggcg gagaatctta tcgctacatt ccagcgttaa
     3301 atgcgagtgg cgatcatatc aaggcattag taaaattaat cgaagcgaaa atataatata
     3361 atttgggcgg ataataaatc cgcccatttt tatctacacc ctataggaat gtacttattt
     3421 tatttaattt tctctaatat ttctctgtta aatcccatca gttctacttt tgtattcggt
     3481 aaattaggga agtcatttaa taaatcttca aacttatcta accaatggta attagtgctg
     3541 gtttgactaa ctaaaaacca cagaatagct acccttccga aaattctctc ctttgctttg
     3601 tcatccagcg ataagtttga aaaatactgc tgcatttttt taaatttcga ccaacgagga
     3661 tcatctagct taggaatagc aatatcgcta aattttctgt tccaaatacg agaatggtga
     3721 gcagaaaggt ttcttacaat gtttatttca ttcaaccaat tctttaacgt attctcatct
     3781 acattaaatt tcttagcaat tttcctttgg tgttgcccat ttaacaatga gtaaaactta
     3841 gataatgcac caaaatccca ggtttcagta attacccaaa aagggatttc cttctcattt
     3901 tttctatgcc attcaataga atcatcttta ctacgcttaa ttttattagc tgtagtttct
     3961 agccaatttg cataagcata attagatttc cattgaaatt ttaagaactt ttgattaatc
     4021 agagaataat cttcataagc caaaggattt tcacgcccca attcatgagc cataatatta
     4081 cgaatattaa cttctaaacg ttcaataata tccaataata aaagccttag ttttttatca
     4141 aaaatatata gcttataaac ttcttcaaat tgtacatttt cgagaaaatt ttcgctaagc
     4201 ccaccatctt gattaggctt tctagatata tgccaaaatc cagataaacg ataataccct
     4261 acctgagata attttttctc tgcatagctt ttatcattta tgagcattcc tctagatatg
     4321 agtagttcta ctaattcgtg atactcttta tgtggttttg taggcataaa attgaaggca
     4381 ttttaagaaa ggcaaggacg tagcttgaca ctctaagtcg attacattta gtaaacctca
     4441 gggacagggg ctacgccacg attgcaaggg attatacatt ttagctgatt aaagtcaaaa
     4501 ggtttcatca attaataata gcaatactgt acttttaagg actataattg caaaaaactg
     4561 ataaaatttt accgcttgtt actgcttacg ggccgctttt acaacacctt ttacacttaa
     4621 tagcttacgg agaatcagct cagaatgctc tttattttcc acagaaattt gtactctcac
     4681 ttgatagata tttccttctc ttgcctcgct attaatgctg ccaatgttgc tatttaattt
     4741 ggcaagcgtt gatgtgatcc ccgcgagtac ccctggttca ttgataatat ccagtacgat
     4801 ttccgtttca aattgaatcg ctttttcttt ggcaatttcc cattccacag gtaaataatt
     4861 actcggctct gttgcgtaat ctttcacatt accacaacaa gcattgtgga ttgccaatcc
     4921 tttacctgaa ctgacgtgag caataatttc atcacccggt ataggtaagc aacagtttgc
     4981 aaaactgata aggcggttct caatcccttc aattttaagc ggttgattat tttctggatt
     5041 accatcagta tcaattaata gtggctcccc taataagcgt tgagcgataa cattactcat
     5101 ttggttacct agaccaattt cagccagtag ctcatcaaag cttgataaac gtagctcgtc
     5161 taatacggat tgaatagttt ctgaacacaa ttcatcaagt gagcgtaaat tcattgcccg
     5221 aactagctgt ctacgcccaa gtgcaatcgc ctcatcgtgc tgctgatttt taagcgattg
     5281 acgaatttta gccctcgcct taccacttac tacaaaattt aaccacgatg catttgggcg
     5341 cttaccttgc tcggtaataa tttccaccgt ctgtcctgtt cgtagcggtt ctgaaagtgg
     5401 atacggattg cgatcaacca tcgcacctac acaacgatcc ccaatgcctg aatgcaccgc
     5461 ataggcaaag tcaatagcgg tagcattcgc cggcaattca acaatacgcc ctttcggggt
     5521 aaagacgaaa atatcgtcag agaacagatc cgatttaacg ctatcaataa attctgctgt
     5581 attgccggca ctgttttgca attcaacaat actttgtagc cattgctggg ctttgagttg
     5641 aacggtcgtg ttatggtgcg actcgccctc tttgtaacgc cagtgagctg ttacccccat
     5701 atccgccatt ttgtccatat cttcggtgcg aatctgtacc tccaccggta caccgtgcgg
     5761 gccaatcatc gaagtgtgta aagattgata gccgttcggt tttggcacgg caatataatc
     5821 tttcacccgt ttcggacgag gcttatacag tgcgtgcatc tgccctaata cacggtaaca
     5881 attatctaca ttatcaacca ctactcggaa agcataaata tctaaaatcg aatgaaaatg
     5941 ttgatctttc tggcgcattt tttggtaaat ggaatagaga tgtttttcac gcccatacac
     6001 acgggcttca atttgcacct cttctaaccg accacgaata tcatcggcaa tgcgttgaat
     6061 tagctcttta cgatgtcctc tggcactggc aatagccatt ttcagtaccc gataacgttg
     6121 cgggtgcatt gcctcgaagc ctaaatcttc caactcgttt ttaatatgct ctattcccaa
     6181 gcgatgtgcc aacgggctat aaatctctaa ggtttctttc gctatacggc ggcgtttatc
     6241 cggacgcaaa gcaccaagcg tacgcatatt atgggttcgg tcggcaagtt taattaacac
     6301 aacccgaatg tctttggtca ttgccaaaat cattttacgg aagtttgcca cttctgcctc
     6361 ttggcgggtg cggaatttta atttatccag cttagaaacg ccttgtacaa tatctgctac
     6421 gctctgtcca aattccgccg ccagttcttc ttcggtgtaa ggtgtatctt caatcacatc
     6481 gtgcaacaaa gccgcaatca ccgcttcttg atcgagcttc atttccgcaa taatcgacgc
     6541 cactgctacc gggtgagtaa tatagggctc accgctagag cgtgtttgtc cctcgtgagc
     6601 atcacgggct atcacataag cacgttttac acgctcaatc tgctcgggtg gcaaatagcc
     6661 ttgaataatt cgatctaaag gttcaaaaag atacacgtta tacctcaaca acagaaataa
     6721 aaatggacaa cgaatttgca aattttgagg aaaatcctac cgcttgcaga ctcaattgtc
     6781 cattctctat ttacgaaaaa taaaattacg cttcgttgct catttcagtt aaaaatgaaa
     6841 tcgcctcttg ctccgccgct tcttgcttaa tagagtcgtg attttccagc tgatccataa
     6901 tgtcagttgt aattaaacct tcttcaattt cacgtaatgc aatcacggtc ggcttatcgt
     6961 tttcttcagg cacaagtggc tcacgggtgt gaagttgtaa ttgtcttgca cgacgtgcag
     7021 caattaagat taaatcaaaa cggttgccaa ttttctccgc tgcttcttgt acggttacac
     7081 gagccataaa tttactccaa agatttattc ggttttattc ataaaaagat tgcgaattat
     7141 acagaaattc cgccaattcc gcaatgaaag cgggctaaat tcacaaaatt tatttggtat
     7201 tcagtaaaat cgccaataat tcagcctcaa cctctttgcc gaattttccc attctaaaat
     7261 cggcttttag ttgctcgaat tgctcagggt tcgctgccat tttatctttt aaaaattgcg
     7321 gaaattcaga taacactaat tctgttttgc cattacgaat atcaaaaata gttggaattt
     7381 tttgcgcaga aatgaccgct tgttgctgtt catcgctgtc aaaatgtttg cgttcctgat
     7441 tatctttgcg agccacatat ttcaccttaa tgttaggatt gagctttgcc attcgctgga
     7501 aataagccac aatttccaca caatcggggc aatatggctc tgccattacc agccagtccc
     7561 cttgcacgcc aacagcttgc agtttttctt ccgtatctgt ggaaagcttg acctccacat
     7621 acacttctaa ttgattttca cgatcttcat cgctgttaaa ttctaaatat tcgtaaaaat
     7681 gtgccataat tttgtctttt cctatctcat cttaaatgga tttacgccat aaaatttcac
     7741 aatcaacttt aacgtcatct attttctgtt gcgctaactt ttgctgaatt aattcaaagg
     7801 tacgttcggc aatttgttgg tggtcttgct taattgaatg aatatgaaaa ggtagtgcat
     7861 tcagtaaatg gtgatcatca aaggttgcta aatgcagttc ttgagtcaat aatttatcca
     7921 tttgttggtg ttcagtcaga tagcgcatta ctccctcaag tagggtaaaa gaggctgtga
     7981 ataccgcttc tgggagcctg ccaagttttt caactatctg agcgaacatt tgatagcccg
     8041 attccggctg atagtcttga tgcagaaccc aattttcatc aagtgccaaa tgtgactgct
     8101 ccaaaccttc acggaagcct tttaaacgat tttgacttgg cgagagtgaa agttgtccac
     8161 ctaagtaaaa aaactctttt aagtgataag cccgtacaat attttccacc aacatactga
     8221 tactttgtgt atcattactg gtgatagaag ggatattcaa cccagaaata tagcggtcta
     8281 gctgtaacat tggggtatat tgtgccgcat tttgataata agtcggctca ttatgtgtcg
     8341 gagcagtaat cagtaaatct acctgtctgt ctaataaacg ctctatcgct ttcttctcac
     8401 cttcaaaggt atcatccgag caagcgatca ctagctgtaa cccgttttca cggcataatt
     8461 tctctaaggt tcttgaggtc agagcaaagc cataattcgt taaatcggga atgactaaac
     8521 cgatcacatt cgtgcgttga gcctgtaatg attttgcata tacattagca cgataaccgt
     8581 gttttttagc gatctctttc actttttggc aggtttcgga tttgattcta aaatcattgc
     8641 cccgcccatt taaaatcata ctgactgttg ttttagatac gccgctgagt tccgcaatat
     8701 cactgagcgt taggcgtttt ttagtcacac ttatctcctt ttagcataaa aatgggcatt
     8761 actgcccatt gctttattct acgctaattg gcaatacttt tgctactttc acttgaatcg
     8821 gacgagagct ttttacgaca ttttcacgct tagcgataaa aaagcgtgaa gtcagcactt
     8881 tttccccgcc gtttacgaaa atttccacca cagaacggtc aaagaaaatc tcgattgtct
     8941 ctaattgttc aattttgcaa tgacgaaccg agccaaattt ttccatcaat tctgtttgtt
     9001 cggtttggct acgatctaag ctcaacaccc cattttcaaa acgtaagctg agcttttgcc
     9061 cctgttcatt gctgaaaaaa tccaacacaa gcggttgatt ttccacagaa atttgcaagt
     9121 aggcagtatc caaattctcg attgcaaaat tttctgacat ttcgaccgct tgtaattcgc
     9181 ctacattcac gattgggttt tgtatcactt tgccgttttg cagggaaagt ttacgaggca
     9241 atgtcagcat tgaatgccac tgatatttat cagttgggta agtgagatca ggcaagccga
     9301 cccagccaaa caaaatgcga tcggaatttt ccaccgtttg cggtgcgtaa aagtcaaagc
     9361 cgtagtccag ctcttcaata tgctctgccc gtaatctgtt gccgtttagc tcgccaagtg
     9421 cgtaagtggc gtggtagtta ttttggaatt gatggctttc acgcagtttg ccttgtggcg
     9481 accacacaaa cagatcttta ccaccaagtg agaacagatc cggacactcc cacataaaca
     9541 cattgctgtt atcaaaattg tggacttcca gttctgcgat taaacggggt gtggcattta
     9601 aatcgtccat ttcataaatt aacgccgtgc cggtcaagtt ttcacgctga gcccccaaca
     9661 caaaacgaat tttgccctct gcggtaaggt aaggtttcgg gtcacgcacg tgttcggtgt
     9721 agcctgccgg tgcttggtcg ataatgcaac gcttttccaa taatttgccc gatttatcaa
     9781 aaatcgcgat attttggtga ggaatgcggg cattatctga tgggcggcgt gtattgccgg
     9841 tgtaaaaagc gacaatctta tcttgccata aaatcgcccc acctgaataa cagccgtggg
     9901 attcaaacag ctcatcggga atcagcaaat cccctttact gaacgtttga aaatcggtag
     9961 tgatgaaatg ctgccaatgt ttcattccat gtaatgcccc ataaggaaac cattgggcga
    10021 aaatatggta tttttcacca tcgaaaatca agccgtttgg gtcattaaat aagcctgttt
    10081 cgggagcgag atgataggtt gggtaaaaat cgggatcaga taaaacggtt tggcgaagag
    10141 cgtttaattc gccctcgctt tgtgcgtgca ggcttttgta tttgccgttg ttgaaaatgt
    10201 gcatagagtg ttcctgtcat ttcagaaatt acgatggtgc aagcgtccac gcttgtaccc
    10261 ttttgtaaaa aatgaggcac aagcgtggac acttgcgcca tcattacatt attccgccaa
    10321 aaatgcgtct aactccgctt tattcggtaa tgccgacatc gccccttttg ctgtggtggc
    10381 taacgcaccg gaagcatttg ctttgcggat cacttcaact aacacagttt catcgtgcca
    10441 attcggcgtt tgagcaagac cggctaataa tccgctgaca aaggcatctc cggcaccggt
    10501 ggtatccaca ggtttcaagg ctttgccggc aacgacttgg ctttttccgt tgaaatggta
    10561 gattgcaccg tctttgccaa gggtgataat aatcaatttc tccggatact gagcggtaat
    10621 agcttgtgtg gcttgttcca aagtttctgt ctcggttaat aaggtcagct cttcttcgga
    10681 aaatttgaga atatccgcca tcgccaccac agaatttacc actgttttca tttcatcaag
    10741 gctcttccag agcgattcac gcaagtttgg atcaaacgaa acatagccac ccgccttttt
    10801 cactcggcga atcccttcaa tggtagtgga acgggatggg tcgttaatca aggcaataga
    10861 acagcagtgt aagaaatcac cttggttaaa ttcaggtaaa tcgccgattt ctaaaaattg
    10921 gtcggcactc ggatttacca taaaagtaaa actacgctca ccgttatcta agccaacaat
    10981 cacggtggaa gtgcggtgct gagcgtctaa aatcatctgt ttggtgcaaa cattttctgc
    11041 ctgtaacact tctcgcataa atttacccag agggtcgtta cccacacgcc cgataaatcc
    11101 tgcctcaacc cctaaacgag aaacgcctac tgcaacattc gccggagcac caccggcaca
    11161 ttttaaatag tgatgttcgc cgtcgggaat taaatccacc accgcatcgc ctgttaccca
    11221 aatcttactc atctcataca cctttaatta gtcacaaaac accaagctaa aacgatttag
    11281 catttattcg attatatact aaaagaccgc ttgtacatac ctacaagcgg tcattttttc
    11341 tttaattttt acagattaac cacgtattta cctacaccta aatcatcttc tttacgagtg
    11401 cgggcaatca cgctttctgc cgattccacc acacgtaatc ccatttggtc ttcggtacga
    11461 atcacttcgc cacggagtga gttggtataa acatcggtga ttttcacatc gacaaatttg
    11521 ccaatcatat ccggtgtgcc aacaaagttt actacacggt tagtttcggt tctgccggtt
    11581 aattccatca agtctttctt agacgggcct tcaaccaaaa tgcgttgttc tgtaccgagc
    11641 attgcacggc taaattgcat tgcttggtgg ttgatgcgtt gttgtaaacg ggataatcgc
    11701 tcttttttct catcatcgga gacattatca ggcagatctg ccgctggtgt acccggtcga
    11761 gccgagtaga taaagctaaa gctcatatca aagttcactt gctcgatcac tttcattgtt
    11821 tgctcgaaat cctctgcggt ttcgcccggg aagcctacaa taaagtccga actaatttga
    11881 atctcaggtc gaacagcacg caatttacgg ataatcgctt tgtattccaa cgctgtgtgg
    11941 ttacgcttca tcattgttaa tacacggtct gaaccacttt gaatcggtaa atgtaagaaa
    12001 ctgaccaatt caggtgtatc acgataaact tcgataatat catcggtaaa ttcaatcggg
    12061 tggctggtgg tgtaacgtaa gcggtcaata ccgtcaatcg ctgccactaa gcgtaataat
    12121 tcggcaaagg tacaaatgcc gccatcaaaa gtttcaccac ggtaagcatt tacgttttga
    12181 ccgagtaaat taacctcacg cacaccttgt gccgcaagtt gggcgatttc aaataatacg
    12241 tcatccaccg ggcgggaaac ctcttcgcca cgagtgtaag gcacaacgca gaacgaacaa
    12301 tatttattgc agccttccat aattgaaacg aatgctgtag ggccttcggc ttttggctcc
    12361 ggcaagcggt caaatttttc aatttccggg aaagaaatat cgacaatcga gcttttaccg
    12421 ccacggattt ggttgatcat ttcaggcaaa cggtgcaaag tttgtggccc gaaaacaata
    12481 tctacataag gggcacgatc acggatatgc tccccttctt gtgaagccac acaaccgcca
    12541 acaccgataa tcaggttcgg tttatctttt ttccagtttt tccaacgccc gagttgcgag
    12601 aatacttttt cttgtgcttt ttcacggata gaacaagtgt ttagcagcaa cacgtctgca
    12661 tcttcagggt tttcagtcag ttcaaaaccg tgagtagagt ttaggagatc cgccattttt
    12721 gatgagtcgt actcattcat ttggcagccc caagtggtaa tatgtaattt agccataacg
    12781 tttaaaatta ataaataatt caataactta gaaaagaaaa gcgtaaagca atttattatt
    12841 tttgacttaa aataaaccct ttacgcaaaa tttgtggcgt tattttacct tgttgcctat
    12901 tttttatcta taaataatcg ccatttatcc gattttacct aaaacaaaaa agccacgatc
    12961 ttccatcgtg gcttccagct aagtaggagg tattatttgg ttaaggttaa aaccacgctt
    13021 tcaccggcaa caacttggcc atttaattta gataagttgc tgatttcatc catatttgaa
    13081 atcacgattg gtgttaatac tgatttggct ttgccttcaa gtagagctaa atcaaattta
    13141 atgatcggct cgcccacttt tacggtttga ccttcctgtg caaggcgagt aaagccttcg
    13201 ccttttaatt caacagtatc aataccgaag tgaacaaata attccacgcc ttcttcagat
    13261 tcaatcgaaa aagcgtggtt ggtttcaaaa attttgccga tagtcccatt tactggagca
    13321 acgatagtgt ctccactcgg gcgaattgcc acaccgtcac ctacaatttt ttcagagaaa
    13381 acaacatcag gcacatcttc aatatttacg atttcacctg ttaatggcgc atagattttg
    13441 atttcttgac tctctttttg ttttgagccg aataatttat caaataaacc catcttaaaa
    13501 tctcccttag atattagata caaaattctc gtacatttta tacgagaatt ttgtatttgt
    13561 atgctaaata attaaaatta gtttaatttc tgataaaact catctactaa gcgttcaata
    13621 tctgcagcag ttggctgagc aagcgcctca tcagctaatg ctttagcatc ggcaaaatta
    13681 attgagcgaa tgagtttttt aacgtgtggc acagagatac cgctcatact aaattcatct
    13741 aatcccatac cgagcaacaa cgcagttgca cgcacgtcac cggctaattc accacacata
    13801 cctgtccatt tgccttcagc gtgtgaagca tcaataactt gcttgattaa gtttaagact
    13861 gatgggctta atgggttata taaatgagca ataatttcat taccacgatc taccgcaagt
    13921 gtatattggg ttaagtcatt agtaccaata ctaaagaagt cggcttcttt tgcaagatga
    13981 cgtgcattta ccgctgctga tggggtttcc accataatac ctagttttaa gttctcatca
    14041 aaagcgtgac cttctgcacg taattgatct ttcaattccg caacgatgga ttttaataaa
    14101 cggatttctt ccaccgaaat aatcatcggg aacattaccg ctaaattacc gaacgcagaa
    14161 gcacgtaata ccgcacgtaa ctgagtatct aaaatttcac ggcgggtgaa accgatacgc
    14221 accgcgcgcc aacctaagaa tgggttcatt tctttcggta aatcaaggta tggtaactct
    14281 ttatcacctc cgatatccat cgtacgcaga atgaccattt gaccattcat tgcttcaaca
    14341 atctctttgt aggcttgaaa ttgctcttct tctcccggta attgagtacg atccataaac
    14401 aaaaactcag tacggtataa accaaccgct tcaccaccgt tgcgtaatac accttccaca
    14461 tcacggatag taccgatatt ccctgccaca tcaactttat ggccatctaa tgtttgagca
    14521 ggcaactctt ttaatttggc taattcttct ttttccgcat ttaatttctc ttgtaatgct
    14581 ttgaaattag cgattttttc ttccgatgga tttaaatgta cttcattgtt cactgcatct
    14641 aaaatcagaa aatcaccagt tttaactagg gaagtaacat tgttagtacc aacaatggca
    14701 ggaagctcta gggaacgagc cataatagaa gtgtgagaag tacgaccacc gacatcggta
    14761 ataaagccta atactttatc taaatttaat tgagctgttt cggaaggggt taagtcataa
    14821 gcgactaaaa taacttcatc ttgaatatca ccaagatcta cgatattcat atttaaaata
    14881 ttgcgtacca agcggttacc aatatcacga atatcacctg cacgttcttt taaatattca
    14941 tcgtcaattt ccgcaagcat tgaagcctgc atctcaatca ctttacttgc tgcaacatcc
    15001 gcagtcacaa gattatcagt aaggtaactc aaaatttctt cttcaagctc ttcatcttct
    15061 aagatcatca agtggccttc gaaaatcgcc tctttttcct cacctaatgt acggcgagct
    15121 ttttctttaa tcgcggttaa ttgagcagct gctttttcgc ggcctgtaaa gaattttgct
    15181 ttttctgcct caacttcgct ttcagtaatt ttgcgagtat ttagtacaat cggctcttct
    15241 tttaaaacca atgcttttcc aaatactgca ccaggggaag ctgcgatacc tgtaatcata
    15301 attgtgtcct taatttaaga tgatgtaaaa atcatctgga tgttacatag caaaacactt
    15361 tattgtagct tattaagcta caataaagtg acataaatct taaatccgag aatagaaaaa
    15421 ttattctaaa gtaggaatca aatcaactaa gaaatcaact gctttttgct catcttcgcc
    15481 gtccgcagaa attgtaattg ttgtgccttg agtcaaacct aatgtttgta atttgaataa
    15541 gctttttgca cttgcacttt taccacctga agtcacagtg atatcagaag caaaaccttt
    15601 agctgctttt acaaattcag ccgctgggcg agtgtgtaaa ccatttggag cggtaataac
    15661 aacatctttt gagtacataa aatcttcctc ttaaatttga aagttaaaaa aatttgtttt
    15721 aatccattta ttttgcgcac gcaactttac tacaaattca aattaaaaac acgctaaaaa
    15781 gcgcaaaaac tcgtcgtatc accctgctaa atagggtgtt ttttaagcta acttaaaaat
    15841 catttgtcga atatcaagat tctagcacac acttaagcgg aaatgttaca ataatttcgc
    15901 taccacttct ttcacttata ttctaaattt ggagcaatat gcttagcaca ttaaccaaaa
    15961 caaccgcatg gtttatctac tttagtatta ttgctctcta tgtttttagt ctatgggcgt
    16021 taatggacta tcaccaaaat gataccttat ttgttttttc cgatataacg gccacggtcg
    16081 cttatagcct acttcaagcc agcctctctg cggtaatttc actattttta ggcagcctac
    16141 tggcaagatc tttcttttat ttggacttta aaggaaaaaa tttactctat aaaaccattt
    16201 cgtttatctg ggcattaccc tcactagtaa ttatttttgc ggtaattggc gtatttggta
    16261 actcaggctg gctagccaaa cttttccaac aactcggttg gaattggcaa tttaatttat
    16321 acggcttgca cggcatcatt ttggctcact gcctgtttaa cattccgttt gtgatgaaat
    16381 attataccgg agggcttcaa ttgactccaa gcagtcgctt tcagcttgcg gctcaattaa
    16441 acctaacagg ttggaattat atccaaattg tggaactacc gataatcaaa actttgctgc
    16501 cttatatgtt tatgactgtt tttctggtct gctttaccag ttttccaatc gtattaatgt
    16561 taggcggtag cccaaaatac agcacgcttg aagttgccat ttatcaagcg gtcacttttg
    16621 aatttgattt tgcaaaagcc ctcatcttaa ttggggtaca atttgtagtg ggcattggct
    16681 tacaattagt aatgaattat atctcgactt ctgcactcaa aaagctaaaa cagcaaccta
    16741 accgagtaga aatttggaaa ccaaagccta ccggactggc taaactcggc ttacaagcgg
    16801 ttattttttt gcaaagtttt gcaataattc taccgcttgt cagcgtcatt tgggcaggac
    16861 taagcgtgtc aaaactcagt gagcgtttgc tcaaccctac tttatggcaa gctactcagt
    16921 attctttata cttaagttta attgcttcta tattcgtaat cacaatcagc tatttgattg
    16981 cacttgaagc tcgccaatta ctctatacaa aagccaagct cagatatagc attctcaccg
    17041 caatcaccct attcccctta cttctgccga tctttttatt atcggttggt ttgtttattt
    17101 tattaatgaa tagtgaactc tcaagcgagg cattactgct actggtcggg atatgcaacg
    17161 gcttaacatt actgccattt atttacccaa tgcttttctc cgcaatgtgg aatagcctca
    17221 cctcgcacga caaattagca caaggacttg ggctaaaggg gcttcgccgt tggtggattg
    17281 tggaaaaaaa tcaactaatt cgaccgcttg ttaccgtatt tgcgttagca atgtcatcga
    17341 gcttaggcag tttcaccgtc attgcttttt tcggcaacgc tgatttcagc tcactccctt
    17401 atttactcta ccaacaactt ggtagctata gaaccgaaga tgctgccgtt accgcactca
    17461 ttctgatgtt atttgcgtta ctaccattct tattcagcaa ggataaacta tgatcgaact
    17521 ggatctccat ttcacttacg aacaaatgaa tatgcacttt cagctttctg tcaaaaaagg
    17581 cgaacgtatt gcagtggttg gcgaaagtgg cgcaggcaaa agcacattac ttaatctaat
    17641 tgccggtttt gaaaaggcag aaagtcagca aagccagctg tggctaaatc agaaaaatca
    17701 tcttgatacc gaagttgcac aacgtcctgt ttcaatgcta ttccaagata acaatatttt
    17761 cccgcactta acggtagaac aaaacatcgg cttagcttta gtaccaagtt tgtcgctcaa
    17821 tccgaaacaa aaagcacagg ttaaacaaat tgccacacaa atggggattg aaaccttcct
    17881 tcagcgtcaa gccgatcagc tatcgggcgg acaaaaacag cgagtggcat tagctcgtac
    17941 tttgttgcgg gataagccga ttttattatt ggacgaaccc ttctccgccc tcgacccgca
    18001 acgccgagcc gaattacagc aaatcgtgtt tgacatttgc caaagcagaa atctaaccct
    18061 gttaatggta actcaccaac tatccgaaag cctatttcta tttaatcggg tgataacggt
    18121 agcagatggg gagattgttt ctgatctctc ccctctttag caaagcagat ctgctaggtt
    18181 ctaaatgtag ggacgccacg ctggcgtacg caacgatgtc aattccaaca atctaacggt
    18241 gaatgatgtt ccgcacggat actagcgtta ttcatttttt tcagaactcg ccccctactt
    18301 tagcaaagag gggaactgta actaatatta cctccctatt ccgctattct cttccccaaa
    18361 atgtgatcta cttcacaaat ttggaaatat tttaaatctt ctctttttta gctaaaccga
    18421 tttagttaaa atgcaaccga ttatttttta tgaaaggtaa attcaaatga actatccaaa
    18481 aattgcagaa caaacgattg caaaattagg tggcaaagaa aacattacca cacttgccca
    18541 ctgtgcaacc cgtttacgct taaccattgc tgatgaatcc aaaattgata aagaggcgat
    18601 tgaaaatatt gaaggcgtga aaggtcaatt ctctactccc ggtcaatacc aaattatttt
    18661 tggatcaggc acggtaaatg aagtgtatgc ccaaatgcaa aaacaaatgg gtatggcaga
    18721 tatgtccacc tctgaagtag cggctgccgg tgcagcaaat cagccattac tacaacgttt
    18781 ggtaaaaggc ttagccgata ttttcgtgcc gatcattccg gctattgtgg ctggcggttt
    18841 gttaatgggt attcactcaa tgctaacggc agtcggtttc ttctgggaag gtgaatctgt
    18901 tgttagccgt tatccgaata tttccgattt agttgatttc atcaacacta ttgctaacgc
    18961 accatttgta ttcttacctg tattactcgg tttctcagct acacgcaaat ttggcggtaa
    19021 tccattctta ggtgcggcac ttggtatgtt attggttcac ccggcacttg cagacggttg
    19081 gaactatgcc aaaaccttaa tggaaggtaa catcaaatac tggaatattt tcggcttaga
    19141 aattgaaaaa gtgggttatc aaggcacggt tattccaact atcgtttcgg cttgggtgtt
    19201 agcaaccctt gagaaaactt tccgtaaatt tgtgccaagc tatttagata acttggtgac
    19261 gccaatgatg tcattattta tcgccggtat tttagctttc actgtaatcg gaccattggg
    19321 tcgtgaagca ggttcagcga tttcagcagg cttaacgtgg ttatatgaca gcttaggctt
    19381 tatcggtgga gcaattttcg gtacattcta cgctccaatc gtgattaccg gtatgcacca
    19441 aacctttatt gcggttgaaa cccaattatt agctgaagta gcacaaaccg gcggtacatt
    19501 tatcttcccg attgcagcaa tgtctaacat tgcacaaggt gcagcctgtt taggtgtagc
    19561 ttttgcaatg aaagatgcca aagtgcgtgg tttagccgtg ccgtcaggta tttcagccct
    19621 gttaggtatc aaccggcaat gttcggagta aatttacgct accgccaagc ctttattgcg
    19681 gcgatgatcg gttcaggttt agccagtgca tttatcgctt tctttaatgt gaaagcaatt
    19741 gccttaggtg cagccggttt cttaggtatt ccgtcgatta aacctgacag ccttgcaatg
    19801 tattctgtcg gtatggtaat ttcatttgta gttgcattta ccatttcagc catttgggtg
    19861 aaaaaagcac aagcgaaata atcaaaaata gatcacaaaa aagggattaa gtgttagctt
    19921 aatccctttt gtttttaaac gccttagctt aacaatgagt ttcgcaagcg gtcatttttc
    19981 gctaaaaatt tgcaaattag ctgttgtgca taattcgaga aatacgccct tcggcaatct
    20041 cttcatcacg aattgtcagc acttcacagc cgtctttggt aactacaatt tgatgctcat
    20101 attgtgcgga gtggctgcgg tctttggttt tcactgtcca accgtcaccc attaagcgga
    20161 cttctttttt gccggcattc accattggct caatcgtaaa caccattcct tcttttaaca
    20221 ctacaccgcc atcatcggta gcataatgca ctacttgtgg gtcgcaatgg aattcagtac
    20281 caataccgtg tccgcaatat tctcgtacta ccgaaaagcc ttctttttcc acatattttt
    20341 gaatcgctgt accaatttct tttaaacgca cccccgcttt cacggtgcga atcccgacat
    20401 aaagggactc tagcgtaacc gccattaagc gtttatcccg cacacttggc tcaccgacta
    20461 catacatcat cgagttatcg ccatagtagc catctttgat aaccgtcaca tcgatgttta
    20521 aaatatcgcc ttttttcagc tttttgtcat cacttggaat accgtgacaa acgacctcat
    20581 ttaacgaaat gcacaccgat tttgggaagc cgtgatagcc caaactggct gaagttgccc
    20641 catttgccaa aatgtattcg tgacaaattt tatctaattc accagttgtt acgccctctt
    20701 tcacataagg ggcgatcatt actaaaacat ctgaagccag tttacaggct actcgtagtt
    20761 tttgaatttc ttcttcgttt cttaatggaa tattattcat tacattgctc tctgctatat
    20821 ctgccataaa aagtaagggg attatacctt ttttcagcac aatccccaaa cctcagttta
    20881 gatttctact ttaaatcaag tttcttcata atgaaattta atatcacacc ataaagcggt
    20941 agaaatagca gtaggctcac aaagagttta aacgcataat cgaccgaacc aatagtaaac
    21001 caatgctctg ccatatattc atcgctactt ttataaaagg cgatagcaaa gaatacaaaa
    21061 gtatcgatta aagtaccaaa aatggttgaa caggttggag caagccacca acgcttacct
    21121 tgtcttaaac tattaaatac tagaacgtct aataattgcc caactaaata agctgcaaag
    21181 ctagccagtg caatacggaa aacaaaaaag ttgaactcag ttaatgcact aaagccttga
    21241 aattgggttt caaagaataa aacggaaatc acatagctta tgacaagtgc cggaaacatc
    21301 acaacaaaaa taatttttct agcaagatct gcaccaaata cacgcacggt taaatcggtc
    21361 gctaagaaaa taaatgggaa tgttaacgtt ccccaagttg tcggaaagct aaattctgtt
    21421 agtggtactt gaacttcaaa gctaatttgc actaaatagt tgctaattgc gatgataaaa
    21481 atatggaaaa gtgaaagtaa aattagagaa cgacgtttgt tctcatccga aaaataagta
    21541 gtaaagttca tattgtatcc tttttcgtta aatgaaaact tgaggtaagg gaactcaagg
    21601 gggcattatt ctatacattt tcccataaaa atcaagctca tattatttca aaaaatgaaa
    21661 caattaatct attaaattag ggtttatcta tttttgacat aaagtaaaat ggcacaactt
    21721 aacacttaca taaggaatag ataatgttac aactgagaaa agccaatgaa cgaggcgatg
    21781 gttcttttag ctggcttaaa agctaccaca ctttttcctt tgccaattat tacgacctaa
    21841 aacatatcca tttttcacat ttacgggtaa ttaacgaaga tttcattgca cctaatcacg
    21901 gttttggcaa gcacccgcac gataatatgg aaattttaac ttatatgtta aaaggacgtg
    21961 tggctcacga agacagtatg ggtaataaaa ccgaagtaaa agctggcgaa tttcaaatta
    22021 tgtctgccgg aaccggggtt ttccattcag aaattaatcc agatccgaat gaaaccttac
    22081 acctttacca gatttggatt atcccaaacc aaaaagatgt gacccctcgc tacaaccaag
    22141 atagatttgc cccgaaagag ggagcaacct tgattctttc gccaaacgca gaagaaggtt
    22201 catttaaaat ttaccaagat atgaagctgt ggcgttatca atacccaaca gccaaatcag
    22261 agcaactggc attagagcca aaccgcagtt actggctaca agtggtgaaa gggaatctaa
    22321 acatcaatgg cgttgaactt tccaccagcg atgccttagg cattcgtcaa gagaaagcac
    22381 ttgaaataaa cactcaagat gacgtggaat ttttactgtt tgatctagtg taaatacaag
    22441 agacaagcgg ttaaatttgt aaaaaatatt actaattcag ctgcttgctc tcattaaaca
    22501 ttatcgcacc aaaattctca ctcctagcgt ttcattttgt tgaagtaagg tatttatccg
    22561 tgctgtttcc acacatacca tattttgata cgccgtttct gtcatcgcac taacggtatt
    22621 atgccaagga ttccaaagta cggtttcggt agcattaata tgctcaactt taatggtgcg
    22681 ctgatttgca aaatcttgaa taaaattcac cgcttgtgcc ggataaatac aatccacatt
    22741 ttcacttatc ttgcgaggtg atggcacttc aatttcttga tttgtcaatt tatcaaaaca
    22801 ttgtgttgct aagccttgta cttccgtttt cgtaatatca ccgacctgaa aataagtatg
    22861 caatgcagcc tgtgccggct ccggtgctaa gtgagtaaaa tacaactcac agacttcgcc
    22921 tagtatcatc tcgagttttg cctgattttc taaccccaaa acaacacgaa tcccatctgc
    22981 tgaggaagta tgttccagca aattccacgc ttgaatacgg gctgtgccgt gagcaggctt
    23041 tttgtctgta ccaaaccaag ggtggcaaat cggaatgccg ccacgaatcg ccacacctgt
    23101 ttggaaaggt tcaatttcac ttaaccataa tacatcttgc tgagtatttt taggttgcca
    23161 actcagtaac tgcgcacctt gcaatgcaat tttagccttc actttggagt gattaatctc
    23221 taaaacatcc aattcattat attttgctaa tgctatttca gggctgatca ttttgattcg
    23281 tagattattc atcgtattgc tcctcatctt gaaagataaa atgtgaagtg gatcacagaa
    23341 ttaaagctta aaccgctacc tctttaaagg taatcgttat aataagggca ttctaacata
    23401 gcgaaggaga aaaaaatgaa aacactcggc gaatttatta ttgaaaaaca agcagaatac
    23461 ccaggggcaa aaggggaatt aagtgggatt ttatcttcta ttcgtttagc cgctaaaatt
    23521 attcatcgag aaatcaaccg tgccggttta agtcaagata ttttaggtac tgcaggttct
    23581 gaaaatgtac agggtgaagc ccaaatgaaa cttgatgtat tcgccaacga aacgatgaaa
    23641 aaagcgttac ttgcacgtga agatgttgcc ggctttgcat cggaagaaga tgataatttt
    23701 gttgcctttg ataatgagcg tgggagaaat gcaaaatata ttttaatgac cgatccgctt
    23761 gatggctcct ccaatattga agtgaatgtt tctgtgggaa caatcttctc tatttaccgc
    23821 cgtgtctccc ctatcggcac cccagtaaca caagaagatt tcttgcagga agggcgtaaa
    23881 caagttgccg ccggttatgt tacttatggt tcttcaacca tgctggttta caccacaggc
    23941 aatggcgtga acggttttac ctatgatccg tcattaggct tatttattct ttcacaccct
    24001 gatctaaaaa cccctaccga aggaaaatat tactccatca atgaagggca gtatgttact
    24061 ttcccacaag gtgtgaaacg ctttattaag tattgccaag aaaccgatga agccatcaaa
    24121 cgtccatact cttcacgcta tatcggctcg ctggtgtccg atttccaccg caatttatta
    24181 aaaggcggta tttatattta cccaacctcc accgtatatc caaaagggaa attgcgttta
    24241 ctctatgaat gcaacccgat tgcgtttctt gccgaacaag ccggaggaat ggcgacagat
    24301 ggtattaatc cgattttaga tattaagcca acagagctac accagcgtgt accgttcttt
    24361 gtcggttcaa cctcaatggt caaacaagcg ggcgaattta tgcaagaatt tgcaaaataa
    24421 tgctcctgct tatcactcaa taaaactttc aacaaagcag ctgcgtattc cgtagctgct
    24481 tttgcctatc ttaagattgt aaatttgtat aaaaaacagg taagacctct tgccaaataa
    24541 ataattatct atagaatacc ccaccttttt actccattaa tattagcaat ttattaacat
    24601 tttatgataa ataaaaactt tccactttct taccttgcca gctttgtggc atttgctgtt
    24661 agtttatctg caacagcctc tgttttcaga gatgatatcg atcttcaata ctaccgtgat
    24721 tttgcggaga ataagggtaa attcaccgta ggtgcaagca atattgaaat taaagataaa
    24781 agtggcaata cattagatgc actcttccca aatgtaccga tgccagattt tagtgctgca
    24841 aaccgtaact tagggatagc aactctagtt gcaccgcaat atttagtcag cgttgcccat
    24901 aatacacaat acaatactgt ggaatttggc gccccaggca ctaatgccga tgcacaccac
    24961 tacacttaca aagtcgttga tcgtaacgac tatggaattg ttgaaggtgg gcaacatcaa
    25021 gattatcaag tgccacgtct caataaatta gtcactgagg tcgcacctgc aacagtgact
    25081 gatctgggta ataatgcttc agcctaccaa gatagtagtc gttttactca ctttgctcgc
    25141 ttaggctctg gtcgtcaaat agtcaaaaat accgagcata aagacaacca aatttcaaca
    25201 tcttaccaat atttaaccgg gggagttcat ttaccaatcg cggtacataa ttccgattac
    25261 tggctagatt ttagaggaaa tgcattaaac aatagcccat atggagcatt aactgcattt
    25321 ggtactcgtg gtgatagcgg ttcaggtgtg tatggctacg atcagaaaac gaaacgctgg
    25381 ctactactgg ctacttacac ctttggtacg cctgcaaaca actactacaa ccgtgccggt
    25441 attatccgtc aggattatca cgataaacaa tttgccgaag atattgccgg cacattaacc
    25501 aatgcgcaac aaaatgccgt ttttgaatgg tctgcacagg gtaaagacag tagcattggt
    25561 aataaaggga aaaacttaac ggtttcgctt gctgatcgtt caattaaaga caatagcgct
    25621 tctccattag gctatggtgg cacacctcag ctacaacttc cgcaagataa caccggaaaa
    25681 accctaaaca ttgaaggaca agatagcact atcgtattaa aaaccgatat tgaccaaggt
    25741 gctggcgcat taaatttcaa tgctaatgca acagttcgcc ctgaaaatga tcaaagctgg
    25801 gtaggagctg gaattgtagt ggcaaaaggt aaacaagtta attggcaagt caaaaaccca
    25861 caaggtgacc gtttatctaa attgggcgaa gggacactac atattaacgg taaaggcgaa
    25921 aaccttggtg acattagtgt tggacaaggc actgtcattt tgacccaaca agcgggtgaa
    25981 aatggcaaaa aatctgcatt taaccaagtt ggtattgtga gcggccgttc aaccgttgta
    26041 ttaaacagtg ctgatcaagt cgatccaaat aagatttact ttggcttccg tggtggtcgt
    26101 ttagatctca acggcaacaa cattgcgttt aatcgtattc aaaatagtga tgatggtgcg
    26161 cgtattgtta ataatcacct acaaaaagct gcaacactca ccattaatgg cccgaagccc
    26221 cctgaagcaa cggacttaaa atggggaacg tggaaagaaa acagtgctga tatttatgaa
    26281 tatatcaacc cacatgctaa taaccgtacc gattacttta cgctcaaagg caatccaaac
    26341 caatatatgc caacgaatgg cgcaagtaat gcccattgga ccttcctgtc ttctaacaag
    26401 gatgcagcag taaaacaagt actcgcgcaa aaaggtctgg agcatcgcta caatagcttt
    26461 aatggtttta ttggcgaaac agataacacc cagcataacg gtcgtttgaa tgtggtgtat
    26521 gatccaaaag cctctacacc accggcaact gcctctgaag taacttgggg taaggggcta
    26581 gtagctggtg cagatattta tctgttcacc aatccgaaaa cgaaaattcg ggaatacttt
    26641 gcattaaaag gtgatccaaa acaacctgtt cctcgcggtg gcttaagcag cgagcattgg
    26701 gaatttttag ctgcagatcg caatcaagcg atcaacaaag tattagcccg taaaaacgca
    26761 ccaatagagc aagaaaagct caatagtatt tataccttct ctggtggttt gaacttaaac
    26821 ggcgatctaa ctgtgaaagg aggtaaagtg ctactttccg gcagaccgac ccctcacgct
    26881 tatgatgcga tcaataagca agatgtagtt tatgccgatg attggcaaaa tcgtcaattt
    26941 aaggctgata atatgcagct aaaccaatac gctcagcttt atgttggtcg taatgtctca
    27001 aacctgcaag caaacttgtc agcgaatgac catgctcagc tacatttagg ctttatcaac
    27061 gagcaaactc caagttgcta ctattctgag tacactggta aaacaagctg tgatacacaa
    27121 gcggtcgttt ctaatgaaat ttttgcaaca ctgcccacaa cacaaattaa tggtgatgtc
    27181 agcttggcag aacagagcca attacacatc ggcaaagcga acctgattgg caccattcaa
    27241 gcagcagcaa caacgagcat tcgtttagct aaccaagcaa gctggacaaa cactggggat
    27301 agccgtactg gcaatttagt ggcagaaaac ggttcaacca tcaacctgaa tgaaaaattt
    27361 gtaacaggcg agataccgac tcgttttaat acgttgatta ttgacggtaa tttccaaggt
    27421 aatgccaaaa tcaactactt aaccgatatt gctgctggca aaggcgatca cctacaagta
    27481 aatggtctcg ccgaggggac tttcactctt gcattacgca acagtggcaa agaagctgaa
    27541 gtggttagcc cgttaagttt attaacctta acccatcagg ctcaggctga taaagctaaa
    27601 gtttcactgg aaaacggtta tgttgattta ggtgcatatc gttatgtgct ggcaaatcga
    27661 agcaacggct atcgcttata caatccgtta aaagatgcta aagatcggaa tcagtcgatt
    27721 gcaacggcga aagcggaatt agaccatgct attgcagaag cggataaaca aaaacaggaa
    27781 atcagccgtt tgaacgcaga agccgagaaa gagcgccaag cagagaaaaa tgctaaacaa
    27841 gccgctgcaa acgcacaatc tcagcttagc caagcaaata gcgaattaac ccgtttgcaa
    27901 caatatgctg attactaccg ccgttactat ccaacgtatt accgccaaat ccaagggcaa
    27961 attacggtag caaaacagaa agtcacacaa gcgagtgctg cactcactac agcagaaaac
    28021 aatgctaaag catccgctgc gcaaattgct aacgtagaac aagcggttga aactgcacaa
    28081 aatattgcaa aacaagtaga agcaaaatta gctaccttac gtataactgc tggaaatagt
    28141 gaagcaattt taaacgctga ggcactaaaa ctttgccaag agaatggtgc aaattgtgag
    28201 catctacaag cacacgcaga cactgacagt agtaatattc aacaaagcga ctgggtaagc
    28261 caatatgcca ataccgctct ttccgagcta tcggcacagg caaattctgc gttgcaaatc
    28321 gggcaagact tagatcgcca actgttcgca aaacacgata aattccacgt ttggagcagt
    28381 atcgaacatc aaaaaaccga acaccaatcg gatttatatc gcccgtatga gcaacaaacc
    28441 aacctcactc agttaggcat agaattaccg cttgcaaacg gtatcaatgc cggtgtgatg
    28501 ctatcccgca accacgctaa tgcggaattt gatgagggag tgaacggtaa atccaactta
    28561 ctgatggcaa gcctatatgg taaatggcat tcggaaaacg gcactttcgt cagcctagac
    28621 ggtagctacg gtaaagcgaa aaaccgtatc gacctgttcg gggagaaccg ctttaaccgt
    28681 catattatgg cgattggggc aaatcttgga cataacttcg acttagccgg cgtacaagtt
    28741 cagcctgctg ttggtacacg ttactatcgc ttcagcgcac aaaattacaa actgggtgaa
    28801 gtagaagtcc gcagcccgaa agcacacttt atggcctacc aagcaggtgt gaaagtcagc
    28861 aaggcctttg acctctccag ctggaaagtt gagcctagcc ttgccgcaca ttatgtcgat
    28921 gccagcagca aacgcttgag tgtggcagtc aatgacaata cctttagcca acgtttcgga
    28981 cgttatctga aaactgaagt gggcgtgggt gttacaaaag gtcaatggca attaagctct
    29041 caccttggtc tattgaaagg taacgaaatc ggcaaacaac attttgccgg cttcaaactg
    29101 ggatatagct ggtaattgct attagcccct aaactaagca atcgcttact ttaggggctt
    29161 tttagtcaat taattagccg gctctttgct tgcctctagc cactgccgaa taaaatcagc
    29221 aagctgttca acatcattaa tatccaaccg attttcccgc tcaagcggat aatcggttgc
    29281 ggtcgcgatt gtccactggt ctaacgccgg caacggtttt tcaatttctt tacggtgtag
    29341 ttgaattttc ggcattgttt cgtgcttaaa gccttccact aaaactaaat ctaccgtctc
    29401 tgggtcaaat tttgcaatta attgtgaaaa ttcgaccgct tgtttagttt ctgctgcctg
    29461 cttagtttcc gtcattaatg cccaacgttc atcacacacg accatagtcg ggtttgctcc
    29521 tgcttctcgc aagcgatagc tgtcttttcc ttttttatca atatcaacat tgtggtggga
    29581 atgcttaatc aagccaaccc gaataccgca agcggtcaat tttgggatta atttttccaa
    29641 taaggtggtt ttgcctgtgc cgctatagcc agtaatcgcc agggttttga tgggatatgg
    29701 attgaaagcc gttaaatctt ctaaacaatt aaaattgcga aatgcggctt tatatgccga
    29761 gaaatccact gccacacttt tttgggtttg aaaaaaccac aataatcggc gttcgccgga
    29821 ggctaaatac gctcttaaat tttccgccac cgaacgatgc accaaggcaa ccgttgggtg
    29881 tgggcggtcg ccatcgtgag cataggcaat ttgagcctga ttaatgcgta atgccgtgtg
    29941 taacttgtgc aatagttggg ttggaataaa cggcgtgtca caaggcacaa acagtaaata
    30001 gtcgctctca atctgctcga acccggaaag cattccactt agtgggcctt gaaaatcggg
    30061 caagctgtct tggtaataag gtaattcagg gtaaagttgt tgatattgtt ccaccgaacg
    30121 attgatattg agccaaatat cctcgatttg tggcgaaagt cgggctaaca ggtgcgaaat
    30181 caacggtttt tgatctaaca gttgcaaccc tttttccgct ccattcattc gccgagccaa
    30241 acctccggct aaaattaccg ctgaaatttt ctcttccatt tccctctcca aacctgaatt
    30301 tttttgccaa atgagggcta aaagtatatt attccgctta ctattcaaca agttttttaa
    30361 gaggcatact atgaaatgca aacgtttaga agagctattg gaactgcttg gcgaacattg
    30421 gcggaaaaac cccgatcttc acttaattga tattctgcaa cagctttcgg tggaagtaag
    30481 cgagccggat aattttcatg ccctgcgtga cgaggttttg atttatcagc tcaaaatgcg
    30541 aaacgccgat aaatttgagc cgattccggg cattaaaaaa gattatgaag atgattttaa
    30601 aaccgcattg ctccgagctc gtggaatttt aaacgattaa gtttgtctta tttctcactt
    30661 atcatatttt tgaaggaatc attatgaaaa aatttgcatt aaaaaccgta ctggttgctc
    30721 tttctgccct ttttgcattt aacgcaaccg cagttgcagc agatccaact gccggcaaag
    30781 agtatattga ggtgcgtaaa gccccatcgg ctcaaaaaga agtggtggaa tttttctcat
    30841 tctactgccc ccactgttac gattttgagc taaattacaa aattccaagc caaatcaaag
    30901 cgaaattgcc tgcagacagc aaattagtgc aatatcacgt caatttctta ggtcgccaat
    30961 ctgaaaactt aacccgtgct tgggcattgg caatggcatt aggagcggaa gataaagtaa
    31021 aaaccgcctt gtttgaagcc gctcaaaaag atgcgtttaa atcaatggac gatattcgtg
    31081 cggtcttcat cgctaacagc attagtgcag aacagtttga cagcggtatc aacagctttg
    31141 cagtaaacgg tttagtcaat aaacaagttc aattagctga agacttccaa attcgtggtg
    31201 taccggcgtt tttcgtgaat ggtcaatacc agcttaatct agaaggattt tccgattcaa
    31261 gctcgaccaa tgattttatc caacgctata ttgacgctgt ggtcttcttg agtaagaaat
    31321 aatcgtcccc atattaagga gatggtttta caagtgctga atagtaaagc tattcagcct
    31381 tttttatatg caaactcgga gaacaaaatg acaaacttat ccattctcaa ccttgtgccg
    31441 attcgtgaag ggcaaactgc caaacaagcg gtcgattcta tggtgaaatt agcaaaacac
    31501 gcagaaaata tcggcattga acgctattgg attgccgaac accataatat gaaaaatctt
    31561 gccagttccg ccacgcaatt attgattcaa cacgccctct cccacaccga aaaattaaga
    31621 gtcggctccg gtggcgtgat gttacccaac cacagccctt atattgtggc agaacaatac
    31681 ggcacgcttg aaacactcta cccaaaccga gtagaactgg ggcttgggcg agcccccggc
    31741 accgatatgc gaaccgcctc tgccttacgc ccagagtttg cctttccccg atgaaatcag
    31801 cgaattaaaa ggctactttg acggtacaaa ccctgtttcc gcctatccgg cacaagggct
    31861 aaatgtgccg ttttatattc taggctcaag ccctgaaagt gcctatttag cggctgaacg
    31921 aggcttgcct tacgccttcg cttcacactt tgcccctcgc tttatggacg atgcaatccg
    31981 catttaccgc tcacatttta agccctctga tgtgttagat aaaccttatg tgattttagg
    32041 tgtgaatgcc attgtggcag aaaccgatga agaagcaaca caactggcga ccacccaaac
    32101 ccagtttttc ttaaatgtag tgaccaatgc ccaacaaaat ctgcaaccac cgctcgaatc
    32161 tgacgacaaa gtgtgggaaa attttgtgaa agcagaaatt gtgccgcatt tcgggccggt
    32221 tgcctttgag caaaatgctc tataccgaca agagaaaacc gttgtccgcc aaatgaccga
    32281 atgctcctta attggtagcc cgcaaagcgt tgaacaacaa cttaaagccc taaaagcacg
    32341 agtggaaatt gatgaaatta tggcggtaaa ctacattttc gatgagcaaa aacagcatca
    32401 atcctacact tggttaaaac aaattacgga taaggtgtaa tatgagcaaa acgatgcaac
    32461 aaaaagcggc tgaggcacat aatatgtgcc ggctaaaagg cgattctatg ttggataatg
    32521 cagaccgcca aattgccttt gaagcggtgt atgagagcga agaactggct caacaagcgg
    32581 tcgaattttt cgcacaaaaa gcaaaatcgg tcgaaaccga accttgtgaa attcaaagcg
    32641 aaattacaca aattgcagaa gggtttctga tgaaaatgtg gatcactttc agctgccaag
    32701 cagaagtgat tctgttccaa atggcagtaa ggtagatata aacaagcggt tatttttgat
    32761 gaaatttttg caaaaaatct tcagaaaata accgcttgta tcattatgcc tgtttaaccg
    32821 cagccgattc actacttggt ttacgccgtt taccgatatt tttggtatct ttgtggcgga
    32881 cttttacttt tggttttgcc gcttcttttt tcgcctcttt tttcttctta atgcgtgctt
    32941 tttctttctt gctggtcaca tttagctcgc catctttcgg ggctttggtg cgaggctcta
    33001 agccctcgat aatgcgaggt tttagtactt cttcggtgta acgtttaatt ttgccgagta
    33061 atttataatc gtgagcttcc accaatgaaa ttgccgaacc ttttttgcca gcacgagcgg
    33121 tacgcccaat acggtgcaag taagtatccg cactataagg taaatcgtag ttcagcacga
    33181 aatcaatatc gtctatatca atgcctcgag ccgccacatc ggttgccact aacacattca
    33241 ctacgccttc ttttaaacgg ttaatagctt ggttacgttg ggtctgagcc atttcacctt
    33301 cgagataagt agaacgaatt ccccgcttac gcaaggtttc gctgagttct cgcacatctt
    33361 ctcggcggcg gacaaacaca atcgctcttt ccaccgaaaa ttcgctgata atacgagcta
    33421 ataatttggt tttatgctct acattatcgg cgtggtaata ccattgctga atttttttac
    33481 gctcacggcg acttggttcg gcatcgactt taatcggatc atttaataat cgctcggcaa
    33541 aatcctctaa taactcgccc tcgagggtcg ctgaaaacaa ccaagtatgc ttacgccaac
    33601 gggtttctgc cgcaattttc tccgcatctt gcccgaagcc catttgcaac attctatctg
    33661 cttcatcaaa aatcagcact tccaccgctc ggcagtcaaa attttcctct ttaatgtatt
    33721 gcattaaacg acccggggta gcaaccacaa tatcttgatt tttattaaag acttcaccgt
    33781 gattttgata cgccacccca ccggtaatag tcgcaatgct caactgagta aatttagcca
    33841 gtgcttgggc ttgctccgcc acctgcattg ccagctctcg ggttggggta agaattaaaa
    33901 tgcgaggagc ccctggtttg cggcgaggat aatctaataa atgctgaatt gccggcagta
    33961 aaaaagcggc ggttttgccg gtaccggtag gggctgaacc gagcagatca cgtccttcta
    34021 acgcagccgg aatcgtggct tgttgaatag cggttgggcg gttatagcct ttgtgtgcta
    34081 aggctttaag taattgaggg cttaaatcaa gctcctcaaa cgtcattaat tgggtcatct
    34141 ctgtttctct acttgtaaat ttgccccagt atatcacatt aactgcttgc cgtgatggtg
    34201 aagaaattta tcctaaaagg ttgaaaaaat tgttacactt acacagagcc gtttaacgga
    34261 caattcgctc acttttactt tatagaagga acattatatg tttgttgaaa tttatggacg
    34321 tttatcttgc ccatactgcg tgcgtgcaaa aacattagcc gagaaaatga aacaagaatt
    34381 aagcgatttt gattttaaat ttattgatat gatcgctgaa aatatttcaa aacaagacct
    34441 agagcctcgt gttggcaagc cggttgcaac tgtaccacaa atttttgtgg ataataacca
    34501 cgttggcggt tgcaccgatt tccaagcctt tgtggaagag aaatttggga ttaccgtata
    34561 gccaaaagcc cgctaacaag cgggcttttt tatacaaaaa tttgcaaatt attgttcttc
    34621 tttcggcaga cgtttaatcg caaaaagtaa accgccccag ataatcgtca ttgccaccac
    34681 catggtaata attgctgctg aactcattac tcatctcctt ttggttcatt tttccacgcc
    34741 atttttgata gcaaataagc caccaccact aagccgattg ccattcccca gccgaaggtg
    34801 tttacaaacc agcttgggta gccttcataa ccttcacgat acactttggc gatttcgctg
    34861 aataacataa atgccagtac gccggaggta ataacgatac ataaacgcca gataaagccg
    34921 actttgaatg aagaaacttc gttcaggtgt ttttcaaggg tgcttaattt ctcgctggtg
    34981 acaatcgcaa tcaaggaaac aaacgccacc gcaacaatac cgaattggtt tacgaattta
    35041 tccatcacat caagcattgg taaaccggtg gttgtaccga atagcaagac agataccacc
    35101 atcatcggaa tgccgactag tagcgtggct ttcacacgac ctgaattgag tttgtcttgc
    35161 accgctgaaa taatcacttc aattaccgag ataaatgagg ttaatgccgc aaaggtgagt
    35221 gaaccaaaga acagcacgcc aactaatgcc cccattggtg cttggttaat aatggttggg
    35281 aaagcgaaga atgccaagcc gataccgcct tttgctacct cgttcacttc ttgccctgca
    35341 gccgttgcca taaagccgag tgccgcaaat acgccaatac ccgctaataa ttcaaaacca
    35401 ctgttcgcaa aacccaccac taagcctgaa ccggttaaat cggtttcttt tttcaggtat
    35461 gaagcatagg taatcataat cccgaagcag atagagagtg agaagaagat ttgaccgtaa
    35521 gcggcaatcc acacacttgg atcagccaat ttttcccagt ttggggtaaa taacgcatct
    35581 aagcctttcg cagcacccgg tagggttaat gaatagccga ccaacactaa gaacatcacg
    35641 atcaactgcg gcattagcag gtttgaggca gcagaaatcc cttttttgat ccccatagcc
    35701 aacacagcaa tagcaatcag ccataccgca attaacggcc ctgtaaccat accgacaaat
    35761 tcaaagctca cgccttgagt aatatcgccc atttttaaga attcaccgat aaagaaatcc
    35821 accggtttat cgccccacgc tccgctgaat gagaagtagg tgtaacttgc tgcccagccg
    35881 agtaccaccg cataataaat cccgatgatc acgttgatca gcacttgcca ccaaccgaag
    35941 gtttcaaatt tcgagctgaa acggcggaaa gcgagcggtg caccaccacg atatttatgc
    36001 ccgatagaat aatctaagaa cagcagcggt aaacctgcgg tcaaaagggc aatgatatac
    36061 ggcagaataa aagccccacc gccgttttca taagtagtat aagggaaacg ccagatgttc
    36121 cctaagccga cagcggaacc aatagccgcc ataataaatg caccacgtcc ggagaacgtt
    36181 tctctttgcg gtgcgtttga tgattgatta gacacggtaa atacctcaaa ctgataaagt
    36241 tttatataaa aaagaattga gttaaatgta aattaatcac actttaagtg cgg
//
LOCUS       DANLRK010000003        35612 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.003, whole genome shotgun sequence.
ACCESSION   DANLRK010000003 DANLRK010000000
VERSION     DANLRK010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 35612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35612
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.003"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            11..2310
                     /locus_tag="PXA33_000087"
                     /pseudo
     CDS             11..2310
                     /locus_tag="PXA33_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005759321.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            complement(2388..3089)
                     /locus_tag="PXA33_000088"
     CDS             complement(2388..3089)
                     /locus_tag="PXA33_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HDL5119472.1"
                     /translation="MDLQNRENFLNKLAQRMGRPRQNVPEPIPVLENNHAITRLTDRP
                     QPQLCQEFIDFARVMMADVVEAKEADLPQAIIDICEKYGGASVIVNNDQRLKDLGITA
                     AIQAKYEDSYVWDFNQAETNFEKARTANIGVVYGEYGLAESGGIVLFSDKEFGRSVSL
                     LPEKSVVVLRKSTVLPRVAQLAQILHEKAQRGERMPSCVNIISGPSATADIELIKVVG
                     VHGPVNKIYLVIDDL"
     gene            complement(3094..4503)
                     /locus_tag="PXA33_000089"
     CDS             complement(3094..4503)
                     /locus_tag="PXA33_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HDL5119473.1"
                     /translation="MSYLQTNTLPFKQRVEQQVNNEIVRKALVKAQETIGANRQRMVD
                     ELGNWEEWRDDAKAIRNHVLANLDAYLYQLSEKVTQNGGHVFFAETAEEATDYIKNVA
                     KAKNAKKIVKSKSMVTEEIGMNHVLEAEGIKVVETDLGEYLLQIVGDKPSHIVVPAIH
                     KDRHRIRQEMHDVLGYQGSETPEEMTAFVRQKIREDFLEADIGISGCNFAVPETGSVC
                     LVTNEGNLRMATTVPKTHIAVMGMERIAPTFKEVDVLITMLARSAVGAKLTAYNTWLT
                     GPRLEGETDGPEEFHLVIVDNGRSKILESEFQEVLRCIRCGACLNTCPAYRQIGGHGY
                     GSIYPGPIGSVISPLLGGYEEFKELPYACSLCTACNSVCPVKIPLAQLILKHREKIAD
                     AGLTPMAERLSIFGFNFANSHPTLWKVGVKVGAKVASKLIKNGKAPVNFGALGEWTKA
                     RDLPTAEGESFREWFNNRG"
     gene            complement(4505..5236)
                     /locus_tag="PXA33_000090"
     CDS             complement(4505..5236)
                     /locus_tag="PXA33_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HDL5119474.1"
                     /translation="MNVNFYVTCIADVMKAGVAKNTVLLLEKLGCKITFLEKQGCCGQ
                     PAINSGYTKQALAGMKNLVETFEANDYPIVAPAGSCVYAIKTYPDHFERFGEKEWAER
                     AKKVADRFYDLTDFIVNKLGVKDVGAYLPGKAVYHPSCSLFRKLGIKDEPIALLQHVK
                     GLELLPIKNQETCCGFGGTFSVKMAEISGEMVKEKVANVDTDEPDYLIGADVSCLMNI
                     GGRLSREGKPIKVMHIAEVLMQGGK"
     gene            complement(5380..6984)
                     /locus_tag="PXA33_000091"
     CDS             complement(5380..6984)
                     /locus_tag="PXA33_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005818537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HDL5119475.1"
                     /translation="MALFLSVFPIVLLIYLMVKRNALPSYVALPLTALLIFVLQLTYF
                     GNDTTLIFANIIAGLGDVLTPITVIFGAILFNRFSEVSGATNTMRKWLGTINPNPVAQ
                     LMIIGWAFAFMIEGASGFGTPAAIAAPILVGLGFKPLQVAMLALVMNSVPVSFGAVGT
                     PTWFGMGPLLKDGLLTDAQVLEIGSITALIHSIAAFIIPVMALRLIVSWKEIRQNIVF
                     IYISIFACVIPYFIIAQFNYEFPSLVAGAIGLLVSVWVANMGIGLAKSENHLDGDKAT
                     FGEVAKALLPTGLLIFILVITRIQQLPLKAMLNDATAWIVSSLGFANFEISQGLIFAL
                     KNILGTNVATSYKLLYVPALIPFVVTVLICLPIFSVSGKNTQEIFGTSFKQVKKPFLA
                     LLGAIVMVKLMLIGGVDSMVQIIGKNFAEFTGQSWTLFASFLGAIGAFFSGSNTVSNL
                     TFGTVQYATAQQVGLSVTLILALQSVGGAMGNMVCINNIVAVCSVTGIEKQEGNIIKK
                     TAVPMFVYGVIAAISALFIVPMFYNL"
     gene            complement(7274..8365)
                     /gene="ychF"
                     /locus_tag="PXA33_000092"
     CDS             complement(7274..8365)
                     /gene="ychF"
                     /locus_tag="PXA33_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HDL5119476.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANYPFCTIEPNTGVV
                     PMPDPRLDALAEIVKPERVLPTTMEFVDIAGLVAGASKGEGLGNKFLANIRETDAIGH
                     VVRCFENDDIVHVAGKIDPADDIEIINTELALADLDSCERAIQRLQKRAKGGDKDAKF
                     ELSIMEKILPVLENAGMIRSVDLDKDELHAIKGYNFLTLKPTMYIANVNEDGFENNPY
                     LDRVREIAEKEGAVVVPVCAAIESEIAELDDEEKVEFLQDLGIEEPGLNRVIRAGYRL
                     LNLQTYFTAGVKEVRAWTVSVGATAPKAAAVIHTDFEKGFIRAEVIAYQDFIDNKGEA
                     GAKEAGKWRLEGKDYIVQDGDVMHFRFNV"
     gene            complement(8551..9138)
                     /gene="pth"
                     /locus_tag="PXA33_000093"
     CDS             complement(8551..9138)
                     /gene="pth"
                     /locus_tag="PXA33_000093"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HDL5119477.1"
                     /translation="MSEIKLIVGLANPGVKYEETRHNAGEWLINELARIYNVSLKEEP
                     KYFGKVAKINTAQGEVRLLVPTTFMNLSGKAVGALANFYRIKPEEILVAHDELDLPPG
                     SVKLKKGGGHGGHNGLKDIIAALGNNKEFYRVRIGIGHPGHKDLVANYVLGKPSPSDQ
                     TLLNSAVDEAARCVEVLFKDGMVKATNRLNSFKAN"
     gene            complement(9211..10086)
                     /locus_tag="PXA33_000094"
     CDS             complement(9211..10086)
                     /locus_tag="PXA33_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HDL5119478.1"
                     /translation="MMKKFIPVMIAALVLSACSSQPAKRKSTPRIKATAAYIPTENSA
                     YVVFDLNQKKFLEGKNFNLVRPIASVTKLMTAVVFLEENRYGASCQTQILPSDADFLK
                     GTTSPLPKNLNIACSELLKAMLVRSDNYAAHALAHATHLNKAQFLARMNSKAREIGMT
                     QTYFGDSSGLSSDNVSTVVDLAKLAGYAASKPEIQQLSNSPLVSVYAGGRYFSMKNTN
                     SMVRSQSYQALVSKTGYTREAGYNLAFIAKGDCRGKRIGVVSLNNDNAASRTDFTESM
                     LAKYQCVPNPYRQYD"
     gene            10308..11888
                     /gene="prfC"
                     /locus_tag="PXA33_000095"
     CDS             10308..11888
                     /gene="prfC"
                     /locus_tag="PXA33_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HDL5119479.1"
                     /translation="MSYPQEVNKRRTFAIISHPDAGKTTITEKVLLYGNAIQTAGSVK
                     GKGSAQHAKSDWMEMEKQRGISITTSVMQFPYNDCLVNLLDTPGHEDFSEDTYRTLTA
                     VDSCLMVIDSAKGVEERTIKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVESVLK
                     IKCAPITWPIGCGKLFKGVYHLYKDETYLYQTGQGHTIQEVRVVKGLNNPELDQAVGD
                     DLAQQLRDELELVQGASHEFDHTEFIKGELTPVFFGTALGNFGVNHFLDGLTEWAPAP
                     QARQSDEREVSPSEEKFSGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLKHVRIG
                     KDVVISDALTFMAGDRSQAEEAYAGDIIGLHNHGTIQIGDTFTQGENFKFTGIPNFAP
                     ELFRRIRLRDPLKQKQLLKGLVQLSEEGAVQVFRPLINNDLIVGAVGVLQFDVVVSRL
                     KTEYGVEAIYETVNVATARWVEGKDAKKFEEFKRKNEANLALDGGDNLTYIAPTMVNL
                     NLAQERYPDVQFFKTREH"
     gene            12147..13721
                     /locus_tag="PXA33_000096"
     CDS             12147..13721
                     /locus_tag="PXA33_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL5119480.1"
                     /translation="MKKWLIPLTVAAFCVSACDDKNSTQATAEKPQVENTQSQKNAEI
                     LPLATVVAPWEINSLDPNQSGVIFQRMNLAETLVEADLNGNLIPALATDWRSNEEGTI
                     WRFTLREGVKFHNDQVLTAQAVVNSLNIAKSKPGVLAKAFIKEIRAVDNKVEIELTKA
                     FVPFPPFLTHYTALILAPESYNEKGEVTQIIGTGAFKPTKIEAPQKLEAVQFEGYWGK
                     KPQVQQANYLASSRSETRMLMAQSEPSSLVFNLDTASVARLKADPNLQLVSGSIARTI
                     QLKMDVAKPFFNDLAIRQALSQAINRKAIAEQVLKIENGAADQILPKAFADWRVDTAE
                     QVVDIAKIKQNLTASGYTFDEQGRLTKDGKPFSFTMRTFSDRPELPVIATILQAQWKQ
                     IGVEVNVSVGNFSEIPAGHQDGSLEMALYALNYGKTLDPYGVIASDFANGGSDWGVMN
                     WQNTEVDNAVKSLETERDPQKAKALKQTVSKIIYNDLPIIPVVYYQQNVVAHKEVKNV
                     TLDPFERRFFLELLSK"
     gene            13941..14873
                     /locus_tag="PXA33_000097"
     CDS             13941..14873
                     /locus_tag="PXA33_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL5119481.1"
                     /translation="MHKILFRRLIQILLVIWSVGTLTFILTRQLSGDMAYRIAASRYG
                     YDQADSAAAEAVRAELGLDQPWWQSYFSWLSDLAQFNLGKSLVTGDPVWSEIQHQFGH
                     TLSLAIVALILAILIGPPLGLLAARKPNGFFDRLTLVFSTLFRSVPAFIIAIGLITLF
                     SAQLKWLPAGGYGCWQHFILPALTLAIGLSTVSVRVTRSAMVQVKGSEFYQFSQLKGL
                     SRWHTFLRHGLPNIAVPVIAYHAVQLVYLIEGVVVVESLFAWPGSGHALVHAIIARDV
                     PMIQGTTLVMGGLFVVLNTIADLLSAWIDPRIKR"
     gene            14883..15665
                     /locus_tag="PXA33_000098"
     CDS             14883..15665
                     /locus_tag="PXA33_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL5119482.1"
                     /translation="MKISISQYLGGTILLAMLGFAFLQPYFYPMDIGFQDLTNILSNP
                     NEIAWFGTDHLGRDMLARLASAIRLSFGLSLFSVICALIAGLITGIMAGFFGGWIDRI
                     FSFICDLVMALPGLLLVLLFAALSPGSFWTLYLGISLVMWVEFFRVIRSISSTMANSS
                     EIQASRLMGMGWFYSFKRHLLPRLLPMIATLSAFSLGNAILALATLGFVSVGLRPPTA
                     ELGLMMTELFPYYYEAPLIFLQPVVAVFLMVLSLQLLSRKIK"
     gene            15754..17166
                     /locus_tag="PXA33_000099"
     CDS             15754..17166
                     /locus_tag="PXA33_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL5119483.1"
                     /translation="MKLLDIQNLSVETENRLQLVEPISLQLKAGKNITILGETGSGKS
                     LLIQAIMGALPDGLIASGEISVKNCKISTESDRLQTLWGKTLVMLPQEPRRSLNPIMA
                     IGKQLWESFHYVAGKPSSQAKTESKAYLAKLGLGESVENYPHQLSGGMAQRASFAIAT
                     AAGGKILLADEPTKGLDPISKGKVIELLKSAYQNGGGLLTITHDIEVAEQLGGTILVM
                     KKGELLEQGEAKALLNNPQHPYTKALISADPKHWQPLQDLQNLSQKQPLVSVKNLSIA
                     RGKKTLFSNLSFDLHKGEVLGIVGHSGIGKSSLADVLCGLLKPKSGEVVWHSHSHKKH
                     QVLKLYQDPPEAFAPNVSLKTLLDDVINKHQLDRLPIPSLLQQLSLNPEILQRNAENV
                     SGGELQRVAILRALLLEPVLLFADEVTSRLDPITQQETIELLINQCRARNCSLILVSH
                     DPYLIEKSCDKVINLEEYVG"
     gene            complement(17253..17996)
                     /gene="rlmB"
                     /locus_tag="PXA33_000100"
     CDS             complement(17253..17996)
                     /gene="rlmB"
                     /locus_tag="PXA33_000100"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HDL5119484.1"
                     /translation="MSEQIYGIHAVKAFLDNAPERLIEVLVLKGREDKRLMPLLNELQ
                     RLGVAIQQVNRQTLDNKSQGEVHQGIIARVVPQKELNEHDLEAILSQKKNPLLLILDG
                     VTDPHNLGACLRTADAAGVDAVIVPKDKSAQLTPTARKVACGAAEVMPLIRVTNLART
                     MRDLQERHNVWIVGTAGEAESGIYEAKLTGSIALVMGAEGDGMRRLTREHCDQLISIP
                     MAGSVSSLNVSVATGVCLFEIVRQKLAGQ"
     gene            complement(18051..19418)
                     /gene="pssA"
                     /locus_tag="PXA33_000101"
     CDS             complement(18051..19418)
                     /gene="pssA"
                     /locus_tag="PXA33_000101"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HDL5119485.1"
                     /translation="MLILNKQRKAEYLLKSLPYLPQVAEQVQFLASSKAFKQQILQLI
                     QTAKKRIYITALYFEKDEAGQEVLAALYQAKRNNPELEINILVDWHRAQRGRIGEAEN
                     LSNADWYAKVKYEQGLPAGQEIAFYGVSINGREVFGVLHLKGFVFDDTILYSGASINN
                     VYLHQFERYRYDRYHVIENKVLADSWVTFIQQHLLTNEAVKRLDTENRPKTAEIRAQI
                     KHFRKQLSREAYLFNGVAENGRLAVSPLVGLGRRKNQLNKVIEALFYQTEQKLTICTP
                     YFNFPHSLRTRIEWLLANGKSVEIIVGDKTANDFYTKPDEKFTMASALPYLYEKNLRA
                     FAKRLDGYVQNKQLTIRLWKDGENSYHLKGVWVDNRYILLTGNNLNPRAWRLDAENAI
                     LISDPQHQLSEKAETELNQICQHTQILTHYSDLEVLTDYPENVRKLLKKFGRVKLDKI
                     VKMLL"
     gene            complement(19488..21035)
                     /locus_tag="PXA33_000102"
     CDS             complement(19488..21035)
                     /locus_tag="PXA33_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tannase/feruloyl esterase family alpha/beta
                     hydrolase"
                     /protein_id="HDL5119486.1"
                     /translation="MMKIIAFLFIFLSVFSHTALANSMAEQCVALKSNSPEKTAIYKA
                     EWVEKSAELPAYCLVQGEIGKRVEVDNVPYGIQFELRLPEQWNEKFLFQGAGGVGGVI
                     FPAIGKLYPHGASGANGLNRGYAVVSNDSGHATRDLVFTADPEARSNYAYASIGKVTT
                     VAKQLIAEFYRKPSKHNYIMGCSNGGREAMMAAMRYPQEFDGVIAGSPGFRVSRSVLA
                     EVWDNRALLAVAPKNGDGDKILSQALTQQDLDVIANGVLTRCDKLDGLADGLINAWEQ
                     CDFQPEMVAKQLGQKKVALIKTIFEGAKNSRGELIYSPWAYDSGINAKGWRDWKLGDS
                     QTAQPNSISFKMGLKSLTHYYLAPRVPSRDPLSVDLDQASEQVKAVGSIHDTDQVDLS
                     AFKQNGGKMLIYQGVSDPIFSAVDLKNWYQSLQQAVENPQHFAKLFFVSAMNHCGRGA
                     TVNDFDMLTALENWVEKGLEPESIAAKAGELYPNKAKQIPLCAYPKVAIYQTGEDPNR
                     LTSYQCR"
     gene            complement(21160..23976)
                     /gene="ileS"
                     /locus_tag="PXA33_000103"
     CDS             complement(21160..23976)
                     /gene="ileS"
                     /locus_tag="PXA33_000103"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011608339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HDL5119487.1"
                     /translation="MTIDYKNTLNLPETGFPMRGDLAKREPDMLKNWYDKNLYQKIRT
                     ASKGKKTFILHDGPPYANGNIHLGHAVNKILKDMILKSKTALGFDTPYVPGWDCHGLP
                     IELKVEGIVGKPNEKISAAEFRQACRDYAKEQVEGQKADFIRMGILGDWDNPYLTMNF
                     NTEANIIRTLGKVIANGHLYKGSKPVHWCLDCGSSLAEAEVEYEDKVSPSIYVRFNAV
                     DVNAVEEKFNAQGKGRGALSAVIWTTTPWTIPSNRAIAINPELDYALVQLGDERVLLA
                     VDLVEDVAKAAGVESAEILATTKGENLELLRFNHPFYDYSVPFIFGDHVTTDGGTGLV
                     HTAPDHGADDFVVARKYNIEMAGLISNDGKFKADTPFFAGLGVFESNDKVVEKLQEVG
                     ALLKLSRIKHSYPHCWRHKTPIIFRATPQWFIGMETQGLRQQALGEIKSVRWIPSWGE
                     ARIDTMVANRPDWCISRQRTWGVPMAMFVHNETEELHPRTLELIEEVAKRVEEKGIQA
                     WWDLDPAELLGEEAKDYRKVPDTLDVWFDSGSTYASVVEQRPEFNGNSADMYLEGSDQ
                     HRGWFMSSLMLSTATNGKAPYKQVLTHGFTVDEKGRKMSKSLGNVIVPSEVWNKNGAD
                     ILRLWVASTDYTGEITVSHNILNSAGESYRRIRNTARFLLANLNGFDPKSDLVQPQEM
                     IALDRWAVACALEAQNDIKEAYDNYQFHTVVQRLMRFCSIEMGSFYLDIIKDRQYTTK
                     ADSLARRSCQTALWHIAEALVRWIAPILSFTADEIWGYLPQVEGRSEFVFTEEFYTGL
                     FGLTEQDKLDDAYWQQILKVRAEVNRVLEQARKDKVIGAGLEAKVTVFANDEIRPLLE
                     QLGNELRFVLITSQAIVKPLAEADIAEGELAGLAVKVENADGEKCPRCWHYATDIGSH
                     SGHEEVCGRCVENVAGEGEKRLFA"
     gene            complement(24079..25041)
                     /gene="ribF"
                     /locus_tag="PXA33_000104"
     CDS             complement(24079..25041)
                     /gene="ribF"
                     /locus_tag="PXA33_000104"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HDL5119488.1"
                     /translation="MQLIRGFHNLDKHIALNQGCVLSIGNFDGVHLGHQNILARLCDR
                     ALDLGLPSVVMLFEPQPREFFAKKSGNSTACNPPARLMRLRDKLKYLEEAGVDFVLCV
                     RFSEKFAQISADDFISDLLAKRLKVRYLSVGDDFRFGAKRAGSFETLRNAGLKYRFAV
                     EESHTHSLNEERISSSLVREALQNNELDLAEKLLGKPYSILGRVAHGNKLGRTIGFPT
                     ANIMLNRLVTPIQGVFAVKVKTKQGSFNGIANVGNRPTINGTKPLLEVHIFDFNASIY
                     GEAIEVIFLKKLRNEIKFPDFEALKAQIEKDRLQAVEFFKEFAR"
     gene            25265..26539
                     /gene="nadR"
                     /locus_tag="PXA33_000105"
     CDS             25265..26539
                     /gene="nadR"
                     /locus_tag="PXA33_000105"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HDL5119489.1"
                     /translation="MTDFAYLQQKRKQLKLKVNDICEQAGVTRAYFNQLVSGKIKNPS
                     ANKLKALHSVLDIVEDSNQRSGVIFGKFYPIHTGHINMIYEAFSKVDVLHVIVCTDSE
                     RDLRLFQESKMKRMPTNEDRLRWVQQIFKYQQKQIFIHHLVEDGIPSYPNGWEDWSGR
                     VKALFEEKSINPTIVFSSEIQDKESYEKYLNLEVQLVDPTRESFNISATQIRNNPFQY
                     WRFIPKEVRPFFVKTIAILGGESSGKSVLVSKLANVFNTTSAWEYGREFVFEQLGGNE
                     QAMQYSDYPQMVLGHQRYVDYAMKHAHKVAIIDTDYITTQAFCIQYEGKAHPFLDSMI
                     REYPFDVTILLSNNTKWVADGLRSLGSKKQRQQFQHLLKKLLEKHHIPYIKIESPSYL
                     DRYNQAKEVVEAILNEEPIPLQFKQYNIHEFE"
     gene            26557..27249
                     /locus_tag="PXA33_000106"
     CDS             26557..27249
                     /locus_tag="PXA33_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDL5119490.1"
                     /translation="MILFAGDPHGSYEHLYPILQQQTEEVALVILGDLQLTTPDELDR
                     LSQYCDIWFIHGNHDSKTVAAFDAIWNSSWKERNIHGKVVDIQGIKIAGIGGVFRGHI
                     WMPPNRPMFFDPIHFCQYSAPEKIWRGGIPLRHRSSIFPSDFEALENEKADILITHEA
                     PKPHPQGFSVINQLARKLGASKIFHGHYHDNFNYGNLNVSRECAIYNIGFRSLADING
                     NYLILGVDDREK"
     gene            27382..28104
                     /gene="arcA"
                     /locus_tag="PXA33_000107"
     CDS             27382..28104
                     /gene="arcA"
                     /locus_tag="PXA33_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HDL5119491.1"
                     /translation="MKIPQILIVEDELVTRNMLKSIFEGEGYQVFQASNGEEMHSVLS
                     TNEINLVLMDINLPGKNGLILAREIRENMKLPLMFLTGRDNEIDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLQRTMIDKENDVVKEQSEIESYRFNGWILDINSHSLISPEGEV
                     NKLPRSEFRALLHFCQHPGKIQTREDLLKKMTGRELKPHDRTVDVTIRRIRKHFEDHP
                     NTPEIIVTIHGEGYRFCGNIEP"
     gene            28124..28642
                     /locus_tag="PXA33_000108"
     CDS             28124..28642
                     /locus_tag="PXA33_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HDL5119492.1"
                     /translation="MKKLTIVALTCLFSTHLLAAPVGNTFTGIGVGVDVTTTKYKVEG
                     VKGKDSTGPALVVDYGIEQGNNFVGVVQGKAKVGSTKVFSDVKQKNKYTIAYQQGYRV
                     GSDLLPYVKVDASSSKIGNETYRGFGFGAGAKYAVSSDVELGAEYTRDNLKRSGTKLK
                     GNAFTANATYRF"
     gene            complement(28720..29949)
                     /gene="serA"
                     /locus_tag="PXA33_000109"
     CDS             complement(28720..29949)
                     /gene="serA"
                     /locus_tag="PXA33_000109"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HDL5119493.1"
                     /translation="MAKISLDKSKIKFLLLEGVHQNAIDVLLAAGYTNIEVHKKALDG
                     QELIDAIKDAHFVGIRSRTFLTEEVLAQAPKLIAIGCFCIGTNQVDLNAAKRRGIPVF
                     NAPFSNTRSVAELVLAEMILLMRKVPTANAEVHRGVWNKSAAGANEVRGKKLGIVGYG
                     HIGSQLSILAEAIGMQVYFYDVENKLPLGNAQQVPTLEHLLSTCDMISLHVPEIDSTK
                     NLMSAERIAQLKEGSVLINAARGTVVDLDALAARIEDGSLRGAAIDVFPEEPASINDP
                     FVSPLCKFDNVILTPHIGGSTSEAQANIGTEVANKFVKYSDNGSTVTAVNFPEVSLPL
                     HTNTKRLLHIHENRPGMLNQINQIFVESNVNIAAQYLQTDAAIGYVVIDVESDNVDEA
                     LQRLKAIEGTIRARVLY"
     gene            complement(29986..30645)
                     /gene="rpiA"
                     /locus_tag="PXA33_000110"
     CDS             complement(29986..30645)
                     /gene="rpiA"
                     /locus_tag="PXA33_000110"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HDL5119494.1"
                     /translation="MDQQEMKKIAAQAALKFVKPDTIVGVGSGSTVNCFIDALASMKD
                     EIKGAVAASKASEQRLRDIGIEVFSANEVTELDVYIDGADEITPQGAMIKGGGAALTR
                     EKIVSSLAKKFICIVDKSKQVDVLGSTFPLPVEVIPMARSYVARQLVALGGSPEYREG
                     VVTDNGNVILDVHNFKIMEPLKMEHTINNIAGVVTNGIFAQRFANVTIVGTPEGAVIL
                     E"
     gene            complement(30790..32124)
                     /gene="glmM"
                     /locus_tag="PXA33_000111"
     CDS             complement(30790..32124)
                     /gene="glmM"
                     /locus_tag="PXA33_000111"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015939592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HDL5119495.1"
                     /translation="MARKYFGTDGVRGEVGKFPITPEFALKLGWAAGKVLATQGTKKV
                     LIGKDTRISGYMLESALEAGLTAAGLSAVFVGPMPTPAIAYLTRTFRAEAGIVISASH
                     NPYYDNGIKFFSSVGEKLPDEIEEAIEAMLDKPMDCVDSANLGRASRINDAAGRYIEF
                     CKGTFPSELSLEGYKIVVDCANGATYHIAPNVMRELGAEVLEIGTKPDGLNINEKCGA
                     TDIKALQKVVVEAGADVGLAYDGDGDRLIMVDHLGNKVDGDQILFILAREALRSGKLQ
                     GGVVGTLMSNMSLELALKELAIPFSRANVGDRYVLEQLKEKGWKLGGENSGHIIVLDK
                     NTTGDGIIASLQVLAAMASHKLSLNDLAKAVPLFPQVLLNVRFEKGAANPLESNEVKA
                     VAADVEKRLAGKGRILLRKSGTEPLIRVMVECEDAVLAQSCAEEIVEAVKRN"
     gene            complement(32150..33682)
                     /gene="ilvA"
                     /locus_tag="PXA33_000112"
     CDS             complement(32150..33682)
                     /gene="ilvA"
                     /locus_tag="PXA33_000112"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HDL5119496.1"
                     /translation="MMNETQHSQSAIDYLRSILSSNIYDLAQVTPLQKMEKLSERLGN
                     SIFIKREDRQPVHSFKLRGAFAMISNLSKAQKEAGVIAASAGNHAQGVALSAKHLGLR
                     ALIVMPQNTPAIKVDAVRGYGGEVLLYGANFDEAKAKAIELSTELGMTFIHPFDSPAV
                     IAGQGTIGLELVQQKPDLDYVFIPVGGGGLIAGIAVLIKQVMPNIKVIGVESKDSACL
                     YHALKQGQPTDLERVGLFADGIAVKRIGDETFRLCQQYVDDVVLVDNDEICASMKLIF
                     ENVRAISEPSGATSLAGLKKYVKEHQLQDKNLACILSGANVNFHTLRFVSERCEIGEK
                     REALLAVTIAEQKGSFLKFCEILGNRAVTEFNYRHSDDKQACIFVGVRISGSAEKVEI
                     IKDLQQNGYDVADLSDDDIAKTHIRYMVGGRASSIQYEQLYSFEFPEQKGALLKFLQM
                     LTNWDISLFHYRAHGADYGDILAAFALNRGDEVELNRHLEQLGYRFQNVSESPAYQYF
                     LK"
     gene            complement(33943..34536)
                     /gene="slyD"
                     /locus_tag="PXA33_000113"
     CDS             complement(33943..34536)
                     /gene="slyD"
                     /locus_tag="PXA33_000113"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL5119497.1"
                     /translation="MNITKDLVPSIAYQVRTQEGVLVDEAPVNQPLEYLHGHNNLVEG
                     LEKALEGKAVGDVFEVRVKPEEGYGEYNDNMVQRVPKDVFMGVDELEVGMRFIADTDL
                     GPLPVVITEVDGDEVVVDGNHMLAGQELLFSVEVVGIREATAEEIAHGHVHGGHEHGC
                     GCGGHDHHHDHDGCCGGHDHDHHHHHGHGGCGCGGHH"
     gene            complement(34639..34779)
                     /locus_tag="PXA33_000114"
     CDS             complement(34639..34779)
                     /locus_tag="PXA33_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5119498.1"
                     /translation="MKKLIKVCGLISATIFLSGCLVTSVVGGVVGTAVDVVDTVTPDI
                     ID"
     gene            complement(34851..35537)
                     /gene="aqpZ"
                     /locus_tag="PXA33_000115"
     CDS             complement(34851..35537)
                     /gene="aqpZ"
                     /locus_tag="PXA33_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HDL5119499.1"
                     /translation="MKKYFAEFFGTFWLVFGGCGSAVLAAGIPELGIGYAGVSLAFGL
                     TVLTMAYAVGHISGGHFNPAVSIGLLVGGRFNAKDLVPYIIAQVIGAIAAGAVLYTIA
                     SGVPTFDATAGFAGNGYGEHSPHGYSLTAALLIEVVLTAFFLIIIMGATDKRAPAGFA
                     PIAIGLALTLIHLISIPVTNTSVNPARSTGVALFQGSWAIEQLWLFWVAPIVGAIIGA
                     VVYRFIAEEK"
BASE COUNT        10320 a         8069 c         6995 g        10228 t
ORIGIN      
        1 gactctaaac gtgttaaaga aaaattatct cactgtatca attctccttg ccattagtgg
       61 agtaggctac gctaatgaaa tctctttaga gactattact gttgatggaa atacgccttc
      121 aaccaaaggg aaattactag gtggtgaact aaatagcaat gaatctgttg ttgatgaaaa
      181 aaattaaaac aaggtagtat tacattaggt aacgctttat ctggtgaatt aggcattcat
      241 tcaagccagt tcggaggcgg tgcaagtacc ccaattattc gtgggcaaga gagtaaacgt
      301 gcaaaaatct tacaaaataa tggcgaaaac ttagatatgt cgggaatgtc tcctgatcat
      361 gcagtaaccg tagatgcgct acttgctaaa cgtattgaaa ttttacgtgg tccaactact
      421 ttactttaca gtgcaggtaa tacggcaggt gtaattaatg tggtagacaa taaaattcca
      481 accgctattc ccgaaaaagg ctatgagggg caatttggtg tgcgttttgg ctcagccagt
      541 aaagagcgtt taacctatgc aggctcaacc tttgcccttg gaaatcattt agccttgcgg
      601 gtacagggaa tgtataacaa agcttccgaa tactatgctc cacactttac gattgaaggc
      661 aaaccttacc accgcgtacc agatagcgat gtacaatcac aaaccggcac agtaagcctt
      721 tcttggattg gagagcgagg acatcttggt attgcttaca ctgatcgccg tgataaatat
      781 ggattaattg gtcacacaca caaatacgat cactatacta ttagtattat tcgccaagcc
      841 gttatgtttg caaaaggtta tttacgtttt tatccgcacc ttgcagaaga aggagatatt
      901 gactataaca accctggtat ccgtttactg catactcata ttccgggagg ctcacattat
      961 ggacaagata ctcacgagca cggcaaacct tggattgata tgcactccaa gcggtatgat
     1021 attgatggaa gtttgcaaaa tccacttcct ggttttgaag aagccaaaat atcagctaac
     1081 tatgtagatt actatcatga tgaaaaagat ggaaaaagag ttgaaaatta cttcaagaac
     1141 aaaggaaaaa atctacgttt cgaattggtt cacaaagagt ggaaagggct aaaaggagct
     1201 attggtgtgc aatataccaa ccaatctacc tcagcacttg cacttgaagc ttcacgagct
     1261 gcaaaagtat ttaataaaca gcctttactc aataatccaa aaacaaaatt atggagtctc
     1321 tttgcgattg aacgccttaa tttaggtgac tttacctttg agttaagtgg gcgggcagaa
     1381 cgtcaaaaaa tagcaatgga ttatgatgtg aaattaattg atcgctggct aggctttaat
     1441 acacctatgc caaacttgga cccacataaa gacaaaggtt attcttatag ctttgcaact
     1501 cattggtatt tcgcaccaaa ccataagtta acattaaatg ctgcacatca agaacgttta
     1561 cctaatgctc aggaactcta tgctcatggt aaacacattg ctttaaatgc atttgaggct
     1621 ggcaataaaa atctaaaaaa agagcgttct aaccaaattg agctatcatt agcttatgtt
     1681 ggcgacaaat gggattataa attaaatctc taccacaccc gctatggcaa ctatatttac
     1741 ccattaactc ttaatgataa tcgtggacct aaatctttta ccgatgaata taacttgaaa
     1801 gtgaatagat attatcaagg cgaagctcgt tttagtgggg cagaaggcga aattggttat
     1861 ctatttacac caaattatcg cttagccgta ttcggagact atgtccgtgg aaagttagtc
     1921 aacttaccta atattgcaat gagttacaac atttggacag gtgaagttga caaatgggct
     1981 tctcagccag acatttctgc acctcgtata ccaccactac gtttaggagc aagatttaat
     2041 gctgacttca acctaaactg gtctggaatg ttagaatatt accgagtatt tgctcagaaa
     2101 aaagtctcga aatacgaaca agttacccct ggccatcatc aagtaaacct tggcgtaacc
     2161 tatagtaatc attttaatca aacagaatac caagtattcc ttaaagtgga taacttgctt
     2221 aatcagaaaa tgtaccaaca tgcctcatat ctaccacata ttccacaaat gggtagaaat
     2281 gcaatgcttg gaatgaatat cagtttttaa tttgtaaatc tctacaaaaa tataaccgct
     2341 tgtaaatttc tatctacaag cggttacttt cagtggattt tttgcaatta gagatcgtca
     2401 attaccaagt aaattttatt taccgggccg tgaacgccaa ctactttaat caactcaata
     2461 tccgcagtcg ccgatgggcc tgagatgata ttcacacaag atggcattct ttcacctcgt
     2521 tgggcttttt cgtgcagaat ttgtgcaagc tgtgccacac gaggtaacac tgtacttttg
     2581 cgtaacacaa ccaccgattt ttcgggcaat aagctcactg aacgcccaaa ttctttatcg
     2641 gaaaacagta caataccgcc agactctgcc aagccatatt caccgtaaac cacaccgata
     2701 ttagcagttc tggctttttc aaagttggtt tcagcttggt tgaaatccca cacatagctg
     2761 tcttcgtatt ttgcttgaat ggctgcggta atcccaagat cctttaaacg ttgatcgtta
     2821 ttcacgatca cactcgcccc gccatatttt tcacaaatat caataatggc ttgtggtaaa
     2881 tccgcctctt ttgcctctac tacatctgcc atcatcactc gggcaaaatc gataaactct
     2941 tggcaaagtt gtggttgagg gcgatcggtt aaacgggtaa tcgcgtggtt gttttccaac
     3001 acaggaattg gctcaggcac attttggcgt ggtctgccca tacgctgtgc caatttattt
     3061 aagaaatttt cacgattttg taaatccatt ttcttatcct ctgttattaa accattcacg
     3121 gaaactttcg ccttcggcag tcggtaaatc acgggctttc gtccattcac ctaacgcccc
     3181 aaaattcacc ggtgctttgc cgtttttaat caatttgctt gccactttcg cccccacttt
     3241 tacgccgact ttccaaaggg ttgggtgtga gttagcgaag ttaaagccaa aaatagacaa
     3301 gcgttctgcc attggcgtta agccggcatc cgcaattttc tcacggtgtt ttaagataag
     3361 ctgtgccagt ggaattttca ctggacaaac actgttacaa gcggtacaaa gtgagcaagc
     3421 atacggcaat tctttaaact cttcgtagcc accgagcaat ggcgaaataa ctgaaccaat
     3481 cgggcccgga tagattgaac catagccgtg accgccaatt tggcgatacg ccgggcaagt
     3541 gttcaaacat gcaccacagc ggatacaacg caacacttct tggaattcgc tttctaagat
     3601 tttagaacga ccgttatcga caatgaccaa gtggaactct tccggaccat cggtttcgcc
     3661 ttcaagtcta ggacctgtca gccaagtgtt gtaagcggtg agttttgccc ctaccgcact
     3721 gcgagccagc atggtaatta atacatcaac ctctttaaag gttggggcga tacgttccat
     3781 acccattact gcaatatgcg ttttcggcac agttgttgcc atacgcagat taccttcgtt
     3841 ggtcactaaa caaaccgagc cggtttccgg caccgcaaag ttacagcctg aaataccgat
     3901 gtctgcctct aagaaatctt cacggatttt ttggcggacg aatgcagtca tctcttcagg
     3961 tgtttccgaa ccttgataac ctagcacatc gtgcatttct tggcggattc tgtggcgatc
     4021 tttgtggatt gcaggtacaa caatatgaga aggtttatcg cccacaattt gcaataaata
     4081 ttcgcccaaa tcggtttcaa ccactttaat gccttctgct tctaaaacgt ggttcatccc
     4141 aatctcttcg gttaccatcg acttcgattt tacgattttc ttcgcatttt tagctttcgc
     4201 cacgtttttg atgtaatcag tggcttcttc ggcggtttcg gcaaagaaaa cgtgtccgcc
     4261 gttttgggtg actttttcgc ttaattggta gagataagcg tctaaattgg ctaaaacgtg
     4321 gttacgaatt gctttcgcat catcacgcca ctcttcccag ttgcccagct catccaccat
     4381 acgctgacgg tttgcaccga ttgtttcctg agccttaact aaggctttac gcacaatctc
     4441 gttattaact tgctgctcaa cacgttgttt aaacggcagg gtgttagttt gtaaataaga
     4501 catcttattt tcctccctgc attaacactt cggcaatgtg catcactttg attggcttac
     4561 cttcacggct taaacgaccg ccaatgttca tcaagcagct tacatctgcc ccgattaaat
     4621 aatccggctc atcggtatct acatttgcga ctttctcttt taccatctcg ccggaaattt
     4681 ctgccatttt gaccgagaat gtgccaccaa aaccacagca agtttcttgg tttttgattg
     4741 gtaataactc taacccttta acgtgctgta ataaagcaat aggctcatct ttaatcccca
     4801 gcttacggaa taagctgcaa gacgggtgat aaactgcttt gcccggtaaa taagccccaa
     4861 catctttcac gcccagtttg ttcacaataa aatcggttaa atcatagaaa cgatcagcca
     4921 ctttcttcgc acgttctgcc cattcttttt cgccaaagcg ttcaaaatga tccggatagg
     4981 ttttgatcgc atatacacaa gaacctgccg gggcgacaat cgggtaatca ttagcttcaa
     5041 aggtttcaac caagtttttc atacctgcta aagcttgctt agtgtaaccg ctgttgatcg
     5101 caggttgtcc acagcaacct tgtttttcta agaaagtgat tttacaacct aatttttcca
     5161 ataaaagcac agtatttttt gcaacccctg ctttcattac atctgcaatg caggttacgt
     5221 aaaaattaac gttcataaag aactcccaca gtaaatataa ataagaatta aactcaaaaa
     5281 atgccgttct aagacggcga aaggaagagg tggatttgca aaaaatttgc taaatctcac
     5341 cgcttccttt ctaaatcaag taagctgatt attaactcat tataggttat agaacatagg
     5401 tactataaac aatgccgaaa tcgcagcaat aacgccatac acgaacattg gcacggctgt
     5461 tttcttaata atattgcctt cttgtttctc aatgccggta acagaacaca cagccacgat
     5521 gttgttaata cacaccatat tccccattgc accaccaact gattgcaatg caagaattaa
     5581 ggtaacagat aacccgactt gctgtgccgt tgcatattgt actgttccga aggttaagtt
     5641 agataccgta tttgaccctg agaagaacgc accaattgca cctaagaacg aggcaaataa
     5701 tgtccaagat tgaccggtaa attctgcgaa gtttttacca atgatctgaa ccattgagtc
     5761 tacaccacca attaacatta attttaccat cacaatggca cctaaaagtg ctaagaatgg
     5821 ttttttcact tgtttaaatg aagtgccaaa aatctcttga gtatttttac ctgataccga
     5881 gaaaatcggt aagcaaatta atacggtcac aacaaatgga atcaacgcag gaacatacag
     5941 taatttatag ctagttgcta cattcgtacc taaaatattt ttcagtgcaa aaattaagcc
     6001 ttggctaatt tcaaagttag cgaaaccaag cgaactgaca atccaagctg ttgcatcatt
     6061 tagcattgct tttaatggaa gttgctgaat acgggtgatt accaaaatga agatcagtaa
     6121 ccctgttggt aataatgctt ttgccacttc accaaaggtc gctttatcgc cgtcaaggtg
     6181 gttctcgctt ttcgctaaac caatacccat atttgctacc cagactgaca ccaataaacc
     6241 aatcgccccg gcaactaatg atgggaattc gtagttaaat tgagcaataa tgaagtaagg
     6301 aatgacacag gcaaaaatgc tgatataaat aaacacgata ttttgacgaa tttctttcca
     6361 gctcacaatt aaacgcaatg ccataactgg aataataaac gcagcaatag agtggatcag
     6421 tgcagtaata gaaccaattt caagcacttg tgcatcggtt agtaaaccat cttttaataa
     6481 tggtcccata ccaaaccaag ttggtgtgcc aaccgcacca aatgacaccg gcacagagtt
     6541 cattactaag gctaacattg ccacttgtaa cggtttaaag cctaaaccaa ccagaattgg
     6601 agctgcaatt gccgccggcg taccgaaacc tgatgcgcct tcaatcataa atgcaaatgc
     6661 ccaaccgata atcatcaact gtgccaccgg attcgggtta attgtcccta accatttacg
     6721 catagtgttg gttgcacctg aaacttcaga gaaacggtta aataaaatcg ccccgaaaat
     6781 aacggtaatt ggggttaata catcacctaa accggcaata atattggcaa agattaacgt
     6841 ggtatcatta ccaaaatagg taagctgaag cacaaaaatt aataacgccg taagtggtaa
     6901 agccacataa gacggtaacg cgttacgttt taccattaaa taaatgagca aaacaattgg
     6961 aaatacgctc aggaatagag ccatactgca cctctttata tgattataaa atgacctaac
     7021 tggtcggata ggtgcgaatt gtactcttaa aaaaaactaa tgtaactagg ttgttacaat
     7081 tttgtgatct agctcgcatt tttaatgatt gtatggttaa aaaagagcca tatttatttt
     7141 catttaatac ttaatagtta gccctattta agacattttt accgaatcga gcaatatgca
     7201 attaattaac aaaaaaaggc cgcttgtaga tcacagatca acaagcggtc ttttttaggc
     7261 aaaaatttgc aaattaaaca ttgaaacgga agtgcattac atcgccgtct tgaacaatat
     7321 aatctttacc ttccaaacgc catttacccg cttcttttgc accggcttca cctttattgt
     7381 ctatgaagtc ttggtaagca attacttccg cacgaataaa gcctttctca aagtcagtgt
     7441 gaatcacggc tgctgctttt ggagcggttg cacctaccga aaccgtccac gcacgcactt
     7501 cttttacccc tgcggtaaag taggtttgta agttaagtaa acggtagcct gcacgaataa
     7561 cacggtttaa gcccggctct tcaatgccta aatcttgtag aaattccact ttttcttcgt
     7621 catcaagctc ggcaatttcc gattcaatcg ctgcacacac cggtacaacc accgcccctt
     7681 ctttttcggc aatttcacgc acacggtcaa gataagggtt attttcaaaa ccgtcttcgt
     7741 tcacgttggc aatatacatt gtcggtttta aagttaagaa gttgtagccc ttaatagcgt
     7801 gcagttcatc cttatctaaa tctaccgaac gaatcatacc ggcattttca agcacaggta
     7861 ggattttttc cataatcgat aattcaaatt ttgcgtcttt atcgccacct ttcgcacgtt
     7921 tttgtaaacg ctgaatggca cgctcgcaac tgtctaaatc ggcaagtgcc aattcggtat
     7981 tgatgatttc aatatcatca gctgggtcaa ttttccccgc aacgtgaacg atgtcatcat
     8041 tttcaaaaca acgcacaacg tggccaatcg catcagtttc acgaatattg gctaagaatt
     8101 tattacctaa accttcgcct ttgcttgcac ctgccactaa gccggcaata tccacaaatt
     8161 ccattgtagt tggtaacact cgctcaggtt tcacaatttc cgccaacgca tctaagcgag
     8221 ggtcaggcat tggtaccaca ccggtatttg gctcaatcgt acagaacgga tagtttgctg
     8281 cctcaatacc tgctttagtt aatgcattga aaagggtcga tttaccgaca tttggtaaac
     8341 ccacaatacc acatttaaat cccatttttt gttccttaga attcccctct ccccttgcgg
     8401 gagagggata aatttgagct tcgttcagaa gcgaaaatga gggagagggc gaaattgcaa
     8461 taatttcctc aaatatgacc gctcccctct ctctgaaaaa tacttctgaa cgaagtattt
     8521 ttcggtctct ctcccacaag ggagagagtt ttaatttgct ttaaaactat ttaaacggtt
     8581 agttgcctta accattccgt ctttaaataa aacttctacg caacgtgcgg cttcatcaac
     8641 cgcactattc agtaatgtct gatcgcttgg tgaaggcttg cccagcacat aattcgccac
     8701 taagtcttta tgccccgggt gtccaatacc aattcgcaca cggtaaaact ctttattatt
     8761 gccaagggcg gcaataatat ctttcaagcc gttatgacca ccgtgtccgc cgcctttttt
     8821 caatttcacc gaaccgggtg gtaaatccaa ttcatcgtga gcgaccaaaa tttcttccgg
     8881 tttaatccga taaaaattgg ctaacgctcc cactgcttta ccacttaaat tcataaaagt
     8941 agtcggcact aataaacgga cttcgccttg agcggtatta atttttgcta ctttgccaaa
     9001 atatttcggc tcttctttta ggctaacgtt atatattcgg gcaagttcgt taatcagcca
     9061 ctcgcctgca ttatggcggg tttcttcata ttttaccccc gggttcgcca gcccgacaat
     9121 taatttaatt tctgacacaa tcttgtctca attcatttaa tttaaaaggc agtattttag
     9181 agaaaaaccg ctattttctc aaggcaacca ttaatcatat tgtctatacg gatttggtac
     9241 gcattgatac ttcgctaaca tcgattcagt gaaatcggtg cgtgaagcag cgttatcatt
     9301 gtttaaacta accacaccaa ttcgcttgcc tctgcaatcc cctttagcaa taaaggcaag
     9361 attataaccc gcctcacggg tataacctgt cttactcact aatgcctgat aggattggga
     9421 acgaaccata ctgttggtgt ttttcataga gaaataacga ccacctgcat aaacactcac
     9481 taatggcgaa ttagacaatt gctgaatttc aggcttactt gccgcatagc ctgccaattt
     9541 agcaagatca accaccgttg aaacattatc cgaagataag cctgagctat caccaaagta
     9601 ggtttgagtc ataccgattt cacgggcttt gctgttcatt cgagccaaaa attgggcttt
     9661 attcaaatgt gttgcgtggg caagggcgtg agcggcatag ttatcggagc gaactagcat
     9721 cgcttttaac aattcactac aggcaatgtt aagatttttc ggcaatgggc tggtcgtgcc
     9781 ttttaagaaa tcagcatctg acggcaaaat ttgggtttgg caactcgccc catagcggtt
     9841 ttcttctaag aaaacgactg ctgtcattaa ttttgtgacc gaagcaatcg ggcgaactaa
     9901 attgaaattc ttaccttcca aaaatttctt ttggtttaag tcaaacacca cataagcgga
     9961 attttcggtt ggaatataag cagcggttgc tttaatccgt ggtgtgcttt tcctttttgc
    10021 cggctgggag gaacaggcgc ttaacaccag agccgcaatc ataacaggaa taaatttttt
    10081 catcataata gattcatcga taataaagtc aaagctggtt attctaccct gtttccaaaa
    10141 ttgtgccaaa gcaagcggtc gatttttaca aaatttttgc aaatctagcc gattttgcaa
    10201 aatttccgcc aaatttaacc gcttgccctc tcgcctcaaa tcagcgaaaa taacgcttaa
    10261 ttttttggcg gactttccgc cctttttatt ttaattggtt gagaaaaatg tcatacccac
    10321 aagaagttaa taaacgtcgc acctttgcga ttatttccca ccccgatgcg ggtaaaacca
    10381 ccattaccga aaaagtattg ctctacggaa atgcaatcca aactgccggc tcggtaaaag
    10441 gcaaaggctc ggcacaacac gctaaatccg actggatgga gatggaaaaa caacgtggaa
    10501 tttcgattac cacctccgtg atgcagttcc cgtacaatga ttgcttggta aacttactcg
    10561 atacaccggg gcacgaagac ttctcggaag atacttatcg tactttgact gcggtagata
    10621 gctgcttaat ggttatcgac tcggcaaaag gggttgagga acgcaccatt aaattaatgg
    10681 aagtgacccg cttgcgtgat acgccaatcc taacctttat gaacaagcta gaccgtgata
    10741 ttcgtgaccc gatggagctg ttagatgaag tggaaagcgt gctgaaaatc aaatgtgcgc
    10801 cgatcacttg gccgattggt tgcggaaaat tgtttaaagg ggtatatcac ctttacaagg
    10861 acgaaaccta cttataccaa accggacaag gtcataccat tcaagaagta cgagtggtta
    10921 aaggcttaaa taatccggaa ctggatcaag ccgttggcga tgatttagcc cagcagttgc
    10981 gtgatgaatt agagctggta caaggcgctt cgcacgaatt cgatcacacc gaatttatca
    11041 aaggcgagct gacaccggtt ttctttggca ctgcactggg taacttcggg gtcaatcatt
    11101 tccttgacgg tttaaccgaa tgggcaccgg caccacaagc ccgtcaatcg gacgaacgtg
    11161 aagtttcacc aagcgaggaa aaattcagcg gtttcgtatt taaaattcaa gcgaatatgg
    11221 atccgaaaca ccgagatcga gttgctttta tgcgtgtcgt ttcaggtaaa tatgaaaaag
    11281 gaatgaagct taaacacgtc cgtatcggca aagatgtggt gatctccgat gccttaacct
    11341 ttatggcggg cgaccgttca caagccgaag aagcctacgc cggcgatatt atcggcttgc
    11401 acaatcacgg cacgatccaa attggtgata ctttcaccca aggcgaaaat ttcaaattca
    11461 ccggtattcc gaacttcgcc cctgagctgt tccgccgaat ccgcctgcgt gatccgctca
    11521 aacagaaaca actgctcaaa ggcttggtac aactttccga agagggtgcg gtgcaagtat
    11581 tccgtccgtt aatcaacaac gatttgatcg tgggagcggt tggggtgcta cagtttgatg
    11641 tggtggtttc acggctgaaa accgaatacg gtgtagaggc aatttatgaa accgtaaacg
    11701 ttgccacagc ccgttgggtc gaaggcaaag atgccaaaaa atttgaagag ttcaaacgca
    11761 aaaacgaggc aaaccttgcc ctagacggcg gagacaacct cacctacatt gctccgacaa
    11821 tggtgaactt aaacctcgcc caagaacgtt accctgacgt acagttcttt aaaacgagag
    11881 agcactaaaa ccaatagggg cgaaaaatct ttcgccccta caaataaagc acaagcggtc
    11941 aatttttttt ccgaatttta caatactagg cattctgttc ctcaaatagc ctaaatattc
    12001 ccctatcaac aatccttatc ttgagattat tctgcctatt aattaaccac tcaaaaatac
    12061 cctcgcagaa aactctactt tttttatgga aatatgctaa agttcgccaa tattcatagc
    12121 cctgattttt ttatggagaa aataaaatga aaaaatggtt gattccatta actgtcgcag
    12181 cgttctgtgt ttctgcttgc gatgataaga acagcactca agcaaccgca gaaaaaccgc
    12241 aagtagagaa tactcaatcg caaaaaaatg cggaaatatt accgcttgca accgtggttg
    12301 ccccatggga aatcaatagc cttgacccga atcaatcagg cgtaattttc cagcgtatga
    12361 acttagcgga aacattggtc gaagccgatc ttaacgggaa tttaattccg gcattggcca
    12421 ctgactggcg cagtaacgaa gagggaacaa tctggcgttt taccttgcgt gaaggggtaa
    12481 aattccataa cgatcaagta cttaccgcac aagcagtggt aaatagtttg aatatcgcaa
    12541 aaagcaaacc gggtgtgtta gcaaaagcct ttatcaagga aattcgagcg gtagataaca
    12601 aagtggaaat tgagctaact aaagcttttg tgccgtttcc tcccttttta acacactaca
    12661 ccgccttaat tttggcacct gaaagctata atgaaaaagg cgaagtgacc caaattatcg
    12721 gtacaggtgc gtttaaaccc acaaaaattg aagctccgca gaaattggaa gcggtgcaat
    12781 ttgagggcta ttggggcaaa aaaccgcaag ttcagcaagc caattattta gccagcagcc
    12841 gtagcgaaac ccgtatgtta atggcacaaa gcgagccgtc atcactggta tttaatttag
    12901 ataccgccag tgtagcaagg ctgaaagccg atcctaacct gcaattagtc agcggatcta
    12961 ttgcccgtac tattcagttg aaaatggacg tggcaaagcc gttttttaac gacttagcga
    13021 ttcgccaagc cttatcgcaa gcgattaatc gcaaggcgat tgctgagcag gtcttaaaaa
    13081 ttgagaacgg agctgccgat caaattttac cgaaagcctt tgccgactgg cgtgttgata
    13141 ccgcagaaca agtggtcgat attgcaaaaa ttaaacaaaa tctgaccgct tccggctata
    13201 cctttgatga acaaggtcgc ttaaccaaag acggcaaacc gtttagcttt accatgcgca
    13261 ccttctcaga tcgtcctgaa ttaccggtga ttgctactat cttacaagct caatggaaac
    13321 aaattggcgt ggaagtgaat gtttcggtgg gtaatttcag cgaaattccg gcggggcatc
    13381 aagacggcag tttagaaatg gctctttatg ccttaaatta tggcaaaacc ttagatccat
    13441 atggcgtaat tgcctccgac ttcgccaatg gtggcagtga ttggggggta atgaattggc
    13501 aaaatactga ggtagacaat gcagtcaaat cgctcgaaac cgagcgtgac ccgcaaaaag
    13561 cgaaagcatt aaagcaaacg gttagcaaaa ttatttacaa tgatttgcca atcattccgg
    13621 tggtttatta ccaacaaaat gtggtggcac ataaagaagt aaaaaatgtg acgctagatc
    13681 catttgaaag acgcttcttt ttggagttat taagtaagta gtttaattca aaatactcct
    13741 cgctttagaa aagggaaggt aggatttttc agtagtaatt tgcgctctta ggtgaataaa
    13801 tagtgacaga attatcgttt tttctcattt ttcacctatt ttctccgctt atttcttctg
    13861 ccaaatcttc ccttcccctc tttgctaaag agggggatca gaacgcataa aatcatcttt
    13921 ttaataaatg aaataataca atgcacaaaa ttctcttccg ccgtcttatc caaattctac
    13981 tggtgatttg gtcggttggc acactcacat ttatcctcac ccgccaactt tcgggtgata
    14041 tggcataccg cattgctgcc agccgctatg gttatgatca agcagatagt gctgctgctg
    14101 aggctgtgcg tgccgagctg ggattagacc aaccttggtg gcaaagctac ttcagctggc
    14161 taagtgactt ggctcaattt aatttaggca aatcattggt gaccggtgat ccggtctggt
    14221 ctgaaattca gcaccaattc gggcatacct taagccttgc gattgtggca cttatcttag
    14281 caatccttat cgggcctccg ctcggtttgt tagccgcccg taagccaaac ggcttttttg
    14341 accgcttgac actggtattc agtaccctgt ttcgctccgt gccggcattt attattgcga
    14401 ttggcttgat tacgctgttt tccgctcaac taaaatggct acctgccggc ggatacggct
    14461 gttggcaaca ttttatcttg cctgccttaa cgcttgcgat tggcttgagt acggtttcgg
    14521 tacgagtcac ccgttctgca atggttcagg taaaaggctc agaattttac caattttcac
    14581 aattaaaagg cttaagccgt tggcatacct ttttacgtca cggtttgccg aatattgccg
    14641 tgccggtcat tgcctaccac gcagtacaat tagtttattt aattgaaggc gttgtggtgg
    14701 tcgaaagcct atttgcttgg cccggcagcg gacacgcact ggttcacgcc atcattgccc
    14761 gtgatgtgcc gatgattcaa ggtacaacgc tcgtgatggg cggattgttt gttgtattaa
    14821 acaccattgc cgatctatta agtgcttgga ttgacccacg aattaagcga taggacatca
    14881 agatgaaaat ttcaatttca caatatttag gcggcactat tctccttgca atgttgggat
    14941 ttgccttttt acagccttat ttttacccga tggatattgg ctttcaagat ttaaccaata
    15001 tcctttccaa tccaaatgaa atcgcttggt tcggcaccga tcatctcggg cgggatatgc
    15061 ttgcccgtct tgcctctgct attcgtcttt cgtttggctt atcactgttt agcgtgattt
    15121 gtgccttaat tgcaggctta attaccggca ttatggcagg ctttttcggt ggctggattg
    15181 accgcatttt cagctttatt tgtgatctag tgatggcact ccccggctta cttttagtat
    15241 tgctctttgc cgccctttct cccggctcat tttggacgct ctatttaggg atttcattgg
    15301 taatgtgggt ggaatttttc cgtgtgatcc gctctatcag ttcaacaatg gcaaacagct
    15361 cggaaattca agcctcacgc ctaatgggaa tgggctggtt ttatagcttc aaacgccact
    15421 tattgccccg tttattaccg atgattgcca ctttatcggc attctcgctc ggcaatgcga
    15481 ttttagctct cgccacactt ggctttgtca gcgtagggct tcgcccgcct acagcagaat
    15541 taggcttgat gatgaccgaa ctattccctt actactacga agcaccgctg atatttttgc
    15601 aaccagtggt tgccgttttc ttaatggtac tcagcttaca attattgtct agaaaaatta
    15661 aataatgcgt aggggcaggt cctgtgcctg cccaaaattc ttattatatt tatgggcggg
    15721 cacagggccc gcccctacag taataaaatg aagatgaaat tactcgatat ccaaaaccta
    15781 tctgttgaaa ccgaaaacag attacagctg gtcgaaccga ttagcctaca attaaaggca
    15841 ggaaaaaata ttactatctt aggcgaaacc ggctcaggca aaagcctgct gattcaagcg
    15901 attatggggg cgttacccga cggcttaatc gcaagcggtg aaatttcggt aaaaaattgc
    15961 aaaatttcca ccgaatccga ccgcttgcaa acattgtggg gtaaaacctt ggtaatgctg
    16021 ccacaagagc ctcgccgctc gcttaatccg attatggcaa tcggcaagca gttgtgggaa
    16081 agttttcact atgtggcagg caaaccatca tctcaagcca aaacggaaag caaagcctat
    16141 ttagccaaac ttgggctggg cgaatcggtt gaaaactacc cacaccagct ttccggtgga
    16201 atggctcaac gtgcctcttt tgcgattgca actgccgccg gtggcaaaat tttacttgcc
    16261 gatgagccga ccaaagggct tgacccaatc agtaaaggca aagtgattga actgttaaaa
    16321 tccgcctacc aaaacggtgg aggcttgctc accattactc acgatattga agttgccgag
    16381 caactcggtg gcactatttt agtgatgaaa aaaggcgaac tgttggagca aggtgaggca
    16441 aaagcgttgt taaataaccc acaacaccct tacaccaaag cacttatcag tgccgatcca
    16501 aaacattggc aaccattgca agatctgcaa aatttaagcc aaaaacaacc gcttgtttcg
    16561 gttaaaaatt tgagtattgc ccgtggtaag aaaacgctgt ttagcaacct ttcctttgac
    16621 ttacacaaag gcgaagtgct tgggattgtc gggcatagtg ggattggcaa aagctcgctt
    16681 gccgatgtac tttgtggttt gcttaagccg aaatcgggcg aggttgtgtg gcacagccac
    16741 agccataaaa aacaccaagt gttgaagctt taccaagacc caccggaagc ctttgctccg
    16801 aatgtcagtc taaaaacctt gcttgatgat gtgattaaca agcaccaatt agatcgactg
    16861 ccgattccaa gtttactgca acaactttcg ctcaacccag aaattttgca acgcaatgcg
    16921 gaaaatgtct ccggcggcga gctacaacga gtagcaattt tgcgagcctt gctcttagaa
    16981 ccggtactac tgtttgccga tgaagtcact tctcggcttg acccaatcac acaacaagaa
    17041 acgatagaat tgctgatcaa ccagtgccga gcgagaaatt gcagtctgat tttggtcagt
    17101 cacgatcctt atctaattga gaaatcgtgc gataaagtga ttaatttgga agagtatgtg
    17161 ggctaacaag cggttcaatt tctgagattt tttgcggctc taaaaacaaa aatgcgaaga
    17221 catcagttct tcgcattttt cttacttatt ccttattgcc cagccagctt ctgccgtaca
    17281 atttcaaaca gacagacccc ggttgcaact gagacattca aagaagatac tgagcccgcc
    17341 attggaatgc tgataagctg gtcgcaatgc tcacgggtaa ggcgacgcat tccgtcgcct
    17401 tctgccccca taactaacgc aatcgagccg gttaattttg cttcataaat gcccgattcc
    17461 gcctcacctg ccgtgccgac aatccagaca ttatgccgtt cttgtaaatc tcgcattgtg
    17521 cgagccagat tggtaactcg aatcagtggc atcacttccg ctgccccaca ggcgactttg
    17581 cgagcggttg gtgtaagctg agcagattta tctttcggca caataaccgc atcaacccct
    17641 gccgcatcag cggtacgcaa acaagcccct aggttgtgag ggtcggtcac gccatctaaa
    17701 atcaacaaga gtggattttt cttttggctt aagatcgcct ctaaatcgtg ttcattcagc
    17761 tctttttgtg gcacgactct ggcaataatc ccttgatgca cttcaccttg cgatttgtta
    17821 tctaaggttt ggcggttcac ttgttgaatt gccaccccta aacgttgtaa ttcatttaat
    17881 agtggcatta agcgtttatc ttcacgccct tttagcacca gcacttcaat taaacgctca
    17941 ggtgcattat ccaaaaaggc tttcactgcg tgaatgccat aaatttgttc gctcatattt
    18001 tatccttaat aattgggcga acccatcagc tcgcccatat aaatacctaa ctaaagcaac
    18061 atcttcacaa ttttatccaa cttaaccctg ccgaattttt tcagcaattt acgcacattt
    18121 tcagggtaat cggttaaaac ttccaaatca ctgtagtgag ttaaaatctg cgtatgttgg
    18181 caaatttggt ttaactccgt ttctgctttt tcacttaatt gatgttgagg atcggaaatt
    18241 aaaattgcat tttcggcatc taaccgccac gctcttgggt taaggttatt gccggtcagc
    18301 aaaatatagc ggttatccac ccaaacccct tttaagtggt agctgttttc gccatctttc
    18361 cataagcgaa tggttaactg tttattttgt acataaccgt ccaaccgttt tgcaaaggct
    18421 cgtaagttct tttcatacaa ataaggcaga gccgatgcca tcgtgaattt ttcgtccggc
    18481 ttggtgtaga aatcattagc ggttttatcc cccacaataa tttcaaccga tttgccgttt
    18541 gccaatagcc attcaatacg ggttctcaga gaatgcggaa aattaaaata cggagtgcaa
    18601 atcgttaatt tctgctcagt ttgataaaac aacgcttcaa tcactttatt taactgattc
    18661 ttacgtcgcc ctaagcctac taaaggagaa accgctaagc gaccattttc agcaacaccg
    18721 ttaaataaat acgcttcccg acttaattgt ttgcggaaat gcttaatttg cgcacgaatt
    18781 tcagccgttt tcgggcgatt ttccgtatct aaacgtttta ccgcttcatt ggttaaaaga
    18841 tgctgttgaa taaacgtcac ccagctatcg gcaagaactt tattttcaat gacgtggtaa
    18901 cggtcataac ggtaacgttc aaattgatgc aaatacacat tattaatgct tgctccgcta
    18961 tacaaaatcg tatcgtcaaa tacgaagcct ttcaaatgca atacaccaaa cacttctcgt
    19021 ccgttaatcg acacaccgta aaaggcaatc tcttgccctg ccggtaaacc ttgttcgtat
    19081 ttaacttttg cataccagtc ggcgttagat aaattctctg cctcaccaat acgcccacgt
    19141 tgggctcggt gccaatcaac caaaatgtta atttccagtt caggattatt tcgcttcgct
    19201 tgatataaag cagctaatac ttcctgcccg gcttcatctt tttcaaagta aagagcggtt
    19261 atataaatac gcttttttgc cgtttgaatc agctgtaaaa tttgttgttt aaacgctttg
    19321 ctcgaggcga gaaactgaac ctgctctgcc acttgtggta agtatggcag gcttttcaat
    19381 aggtattcag ctttacgttg tttgttcaaa attaacatca ttgattccaa aattttcaat
    19441 cgttgcctgc tattttacac tgaaatcgcc aataaagcga ttttcgctta acggcattga
    19501 tagctggtca aacgattagg atcttctccg gtttgataaa ttgcgacttt cggataagca
    19561 caaagcggga tctgcttggc tttattaggg taaagctcac cggctttggc ggcaatactt
    19621 tccggctcca gtcctttctc aacccaattt tcaagggcag tgagcatatc aaaatcattc
    19681 accgttgccc ctcgcccaca atggttcatt gccgacacga aaaacaattt tgcaaaatgt
    19741 tgcggatttt cgaccgcttg ttgtaggctt tgataccaat ttttgagatc aactgccgaa
    19801 aaaatcgggt ctgaaacgcc ttgataaatc agcattttgc caccgttctg cttaaaagcg
    19861 gataaatcaa cttgatctgt atcgtgaatg ctccctaccg ctttcacttg ttcgctggct
    19921 tgatccaaat ctacgctgag tggatcacgg ctcggcactc tgggggctaa atagtaatgg
    19981 gtcaaacttt tcaatcccat tttaaaacta atgctgttgg gctgagcggt ttggctatca
    20041 cctaatttcc aatcccgcca gcctttagcg ttaatgccgg aatcatacgc ccaagggcta
    20101 taaatcagct cgcctcgact gtttttcgcc ccctcgaaaa tcgttttgat taaagcgact
    20161 tttttctgcc ccagttgctt tgccaccatt tccggctgga aatcacactg ttcccacgca
    20221 ttaattaaac cgtctgctaa gccgtctaac ttatcgcaac gtgttaatac gccattggca
    20281 atcacatcta aatcttgttg agtgagggct tgggataaaa ttttgtcgcc atcaccattc
    20341 ttcggagcaa ctgccaacaa ggctcgatta tcccatactt ccgccaagac tgaacgggaa
    20401 acccgaaagc cggggctgcc tgcaatcaca ccgtcaaatt cctgtggata acgcattgcc
    20461 gccatcatcg cctcacgacc accattcgag cagcccataa tgtaattgtg cttgctcggt
    20521 tttcggtaaa attcggcaat caactgcttg gcaaccgtgg tcactttgcc gatagaggca
    20581 taggcataat ttgagcgagc ttcgggatct gcggtaaaca ctaaatctcg agtggcgtga
    20641 ccactgtcgt tactcactac tgcatagcct ctgtttaagc catttgcacc actcgccccg
    20701 tgcggataga gcttaccaat ggcagggaaa atcaccccac ctacaccgcc cgccccctga
    20761 aataagaatt tttcattcca ttgttcgggt aaacgtagct caaattgaat accgtaagga
    20821 acattatcca cctccacccg ttttccaatt tcaccttgta ctaaacagta agccggcaac
    20881 tcagcagatt tctcaaccca ttcagcttta taaatagccg ttttttccgg cgagttagat
    20941 tttaacgcga cacattgttc agccatagaa ttagccaaag cagtgtgaga aaaaacggag
    21001 agaaaaataa aaagaaaagc gattattttc atcatttaca tcaatacaag cggttatttt
    21061 tccgaaaatt tttgcaaatt ttaaactgag tgtctatcta tcgctggaaa agctaaggca
    21121 gagtttcctc tgccttagaa agatgaattg aattacattt taagcaaaca agcgtttttc
    21181 gccttcgccg gctacgtttt ccacacaacg tccgcaaact tcttcgtggc cagagtgcga
    21241 gccgatgtcg gtagcataat gccagcaacg agggcatttc tcaccatctg cattttccac
    21301 ttttactgcg agtccggcta attcgccttc cgcaatatct gcttccgcca gtggtttaac
    21361 aatcgcttgt gaggtaatca gcacaaagcg taattcattg ccaagttgtt ctaataacgg
    21421 gcgaatttca tcattggcga ataccgtcac cttggcttct aagccagcac caattacttt
    21481 gtctttacgg gcttgttcca gcacacggtt gacttccgca cgcactttta agatttgttg
    21541 ccagtaagca tcatctaatt tatcttgctc ggttaaaccg aataatccgg tgtagaattc
    21601 ttcggtaaag acaaattcac ttctgccttc cacctgcggt aagtagcccc aaatttcatc
    21661 ggctgtgaag gataaaatcg gtgcaatcca acgtactaac gcttctgcaa tatgccataa
    21721 cgctgtttgg cagctacggc gggcaaggct gtcggctttg gtggtgtatt ggcggtcttt
    21781 gataatatct aagtagaaag accccatttc aatcgagcag aaacgcatta aacgctgtac
    21841 taccgtgtgg aattggtagt tatcataggc ttctttgatg tcgttttgag cctctaaagc
    21901 acaagccacc gcccagcggt ctaaggcgat catctcttgc ggttgaacta aatcactttt
    21961 tggatcgaaa ccgttcaagt tcgccaataa gaaacgggct gtgttacgaa tacggcggta
    22021 tgattccccg gcactgttta aaatattgtg cgaaacggta atctcaccgg tatagtcggt
    22081 ggaggcaacc cataagcgta aaatatctgc accgtttttg ttccacactt cacttggcac
    22141 aattacgttg ccaagcgatt tggacatttt tctgcctttc tcatctacgg taaaaccgtg
    22201 agttagcact tgtttgtatg gtgctttgcc gttagtagca gtggaaagca ttaaagatga
    22261 cataaaccaa ccacgatgtt ggtctgaacc ttctaaatac atatccgctg aattgccgtt
    22321 aaattcaggg cgttgttcta caacagaggc ataggtcgat cctgaatcaa accacacgtc
    22381 taaggtatcc ggcactttgc ggtaatcttt ggcttcttca ccgagcaatt ccgcaggatc
    22441 taaatcccac cacgcttgaa tgcctttttc ttctacacgt ttcgccactt cttcaattaa
    22501 ttctaaagtg cgaggatgta attcttcggt ttcattatgc acaaacatcg ccattggcac
    22561 gccccaagta cgttggcgtg aaatacacca gtctgggcgg tttgccacca tcgtatcaat
    22621 acgagcttca ccccagcttg ggatccaacg caccgatttg atttctccta gtgcttgctg
    22681 gcgtaagcct tgggtttcca ttccgataaa ccattgtggg gtagcacgga agataatcgg
    22741 ggttttgtgt ctccaacagt gcggataact atgcttgatg cgggataatt ttaataatgc
    22801 tcccacttct tgtaattttt cgactacttt atcgttggat tcaaacacac ctaagccggc
    22861 aaagaacggg gtgtctgctt tgaatttacc gtcattggaa attagccctg ccatttcaat
    22921 gttatatttg cgagcgacca cgaagtcgtc cgcaccgtga tccggtgcag tatgcactaa
    22981 ccctgtacca ccgtctgtgg taacgtggtc gccaaaaata aatggcacgc tgtaatcata
    23041 gaatgggtgg ttaaagcgta ataattctaa gttttcgcct ttggtggttg ctaaaatctc
    23101 agcagattca acgcctgctg cttttgcaac atcttcgact aaatcgaccg ctagtaacac
    23161 acgctcatca cctaattgga ctaaggcata gtctaattca gggttgatcg caatcgcacg
    23221 gttagacgga atagtccaag gggtagttgt ccaaataacc gcagataatg cccctctacc
    23281 tttaccctgt gcgttgaatt tttcttctac cgcatttacg tccacagcat taaaacgcac
    23341 ataaatagag ggagaaactt tatcttcata ttccacttca gcttccgcta acgaagatcc
    23401 acaatctaag caccaatgta ccggttttga acctttgtat aaatgcccgt tcgcaatcac
    23461 tttgccgagc gtgcgaataa tattcgcctc agtgttaaaa ttcattgtta ggtatgggtt
    23521 atcccaatca cctaaaatac ccatacggat aaaatcggct ttctgacctt ctacttgttc
    23581 tttcgcatag tcacggcacg cttggcgaaa ctcggcagcc gaaattttct catttggttt
    23641 accaacaatc ccttctactt tcagctcaat cggcaaaccg tgacagtccc agcccggcac
    23701 ataaggggta tcgaaaccta atgcggtttt cgatttcaaa atcatatctt ttaaaatttt
    23761 attcactgcg tgacctaagt gaatattacc gttagcatac ggaggaccat catgcaaaat
    23821 aaaggttttc ttgcctttcg aggcagtacg aattttttgg tataaatttt tgtcatacca
    23881 attttttaac atatcgggtt cacgtttcgc gagatcccca cgcataggaa agcctgtttc
    23941 aggcaagttt aaggtgtttt tgtagtcaat agtcattttt ttatttctcg ttaaaattat
    24001 ctgttttatg cctagcacgg catagccgta ctaggttaag catagctcag cccctctggg
    24061 gctggaaact agaaaggctc accttgcaaa ttccttaaaa aattcaaccg cttgcaagcg
    24121 atctttctca atctgggctt ttagtgcttc aaagtcggga aattttattt cattgcgtaa
    24181 tttttttaaa aagatcactt caatcgcctc accgtaaata gaggcattga aatcaaaaat
    24241 atgcacttct aataacggct tagtaccgtt aatggtcggg cggttgccga cattggcgat
    24301 tccgttaaaa ctgccctgtt tggtcttcac ttttaccgca aatacccctt gaatcggcgt
    24361 aaccaaacga ttgagcataa tatttgcggt aggaaaacca atggttctgc cgagtttatt
    24421 accgtgtgca acgcgtccca gaatcgaata aggcttgccg agtaatttct ctgccaaatc
    24481 taactcattg ttttgtaagg cttctcgcac taatgagctg ctaattcttt cttcgtttaa
    24541 gctatgggta tggctttctt ccaccgcaaa gcgatatttt aagccggcat tgcgtaaagt
    24601 ttcaaaactg cccgcccgtt ttgccccaaa gcgaaaatca tcgcctacac ttaaatagcg
    24661 tactttcagt cttttagcga gcaaatcgct gatgaaatcg tctgccgaaa tttgagcaaa
    24721 tttttcacta aaacgcacgc atagcacaaa atccacaccg gcttcttcca aatattttag
    24781 cttatctcgc aggcgcatta agcgggcggg cgggttacaa gcggttgaat ttccactttt
    24841 ttttgcaaaa aactctcgag gttgtggctc aaataacatc acaactgagg gcaatcctaa
    24901 atctaaagct ctatcacaaa gacgagccaa aatattttga tgcccaagat gcacaccatc
    24961 gaaattcccg atagagagaa cacagccttg atttaaagcg atgtgtttgt ctaagttgtg
    25021 gaaacctcga ataagttgca taaaagataa accattttat aagtgaaaat tgctgtgatt
    25081 ataacgagaa atctacaaaa aaggaaaaaa attcaatctg ttatattttt ttagtgctga
    25141 aagtggtcgt gtagctaatt attaaagcaa agatatgcta taaaactgct atttatacaa
    25201 taaaaatata ttcaaatgct acattttact atatgataat catcaaaaaa tcgagggaat
    25261 cattatgaca gattttgctt atcttcaaca aaagcgcaaa cagcttaaac tcaaagtgaa
    25321 tgatatttgt gaacaagctg gggtaacccg agcttatttt aatcagttag taagcggaaa
    25381 aatcaaaaat ccaagcgcca ataagcttaa agccttgcat tccgttttag atatcgtaga
    25441 agatagcaac cagcgttccg gggttatttt tggcaaattc tatcctattc acacaggtca
    25501 tattaatatg atctatgaag catttagtaa agtagatgtt ttacatgtta ttgtttgtac
    25561 cgattctgaa cgggatttac gtcttttcca ggaaagtaaa atgaaacgaa tgccgacaaa
    25621 tgaagatcgg ctacgctggg tacagcaaat ttttaaatac caacaaaaac agatctttat
    25681 tcaccattta gttgaagacg gtattccgag ctatccaaat ggctgggaag attggtctgg
    25741 tcgtgttaaa gcgctatttg aagaaaaaag cattaatcct actattgttt ttagtagtga
    25801 aattcaagat aaagaatctt atgaaaaata tttgaatcta gaagttcaac ttgtcgatcc
    25861 tactcgtgaa tcctttaata tttcagcaac acaaattcgt aataatcctt ttcaatactg
    25921 gcgctttatt ccgaaagaag ttcgtccttt ctttgttaaa actattgcga ttttaggtgg
    25981 tgaaagcagt ggtaaatcag tgttggtgag caaattagcg aatgtattta acactacctc
    26041 tgcttgggaa tatgggcgtg agtttgtgtt tgagcaactt ggtggcaatg aacaagcaat
    26101 gcaatattcc gactatccgc aaatggtgct tggacatcaa cgctacgttg attatgccat
    26161 gaaacatgct cataaagtgg ctattattga taccgattac atcacaactc aagcgttctg
    26221 tattcagtat gaagggaagg ctcacccgtt tctggattca atgatccgcg aatatccttt
    26281 tgatgtgacg attttacttt ctaacaatac aaaatgggta gccgatggtt tacgcagttt
    26341 aggatcgaaa aaacagcgtc aacagtttca acacctactg aaaaaactac tagaaaaaca
    26401 ccatattcct tatattaaaa ttgaatctcc aagttatttg gatcgctaca atcaagccaa
    26461 agaggtggta gaagctattt taaatgaaga accaatccca ttacagttta agcaatataa
    26521 tattcacgaa tttgaataac tgccacagga aataacatga tactttttgc aggcgatcca
    26581 cacggtagct acgagcatct ttatccgatt ctacaacagc agacggagga ggtagcttta
    26641 gttattctag gcgatcttca attgactact cctgatgaat tagatcggct ttcgcagtat
    26701 tgtgatattt ggtttattca tggtaaccat gacagtaaaa ccgttgctgc ctttgatgct
    26761 atttggaata gtagctggaa agaacgcaat attcacggta aagtagttga cattcaagga
    26821 ataaaaattg caggtattgg aggggtcttc cgaggtcata tttggatgcc gcccaatcgt
    26881 ccaatgtttt ttgatcccat ccatttttgc caatactctg ctccggaaaa aatttggcgt
    26941 ggtggtattc cattaaggca ccgctcttcg atcttccctt ctgattttga agcattggaa
    27001 aatgaaaaag ccgatatttt aattacacac gaagcaccaa agccacaccc tcaaggcttc
    27061 tctgtaatca accagctagc aagaaaatta ggggcgagca aaattttcca tggccactac
    27121 catgataatt ttaattatgg caatctaaat gtcagcagag aatgtgctat ttataatatc
    27181 ggctttcgta gcctagcgga tattaatgga aattatctaa ttttaggtgt tgatgatcga
    27241 gagaaatagc caaaaatttg actgaattaa acaaattgtc attttaccct tcccattcat
    27301 aaatttataa gtaaaatgcg agaaatttgt aacataaacg ttattaaaca gaatttataa
    27361 attaaatcag aggtttaaaa gatgaagatc ccacaaatct taattgttga ggatgagtta
    27421 gtaacaagga atatgctgaa aagcattttt gaaggagaag gatatcaggt atttcaagca
    27481 tctaacggcg aggaaatgca ctctgttctg agtacaaatg aaatcaatct tgtgctaatg
    27541 gacattaact taccgggaaa aaatggatta attctcgccc gagaaattcg agagaatatg
    27601 aagctaccat taatgttttt aaccggtcgc gacaatgaaa ttgataagat cctcggttta
    27661 gaaataggtg ccgatgacta tatcactaaa ccgtttaatc ctcgagaatt gactatccga
    27721 gcgagaaatt tattgcaacg aacgatgata gataaagaaa acgacgttgt aaaagagcaa
    27781 tctgaaattg aaagctatcg ctttaacggt tggatcttag acattaacag tcatagttta
    27841 atctcaccag aaggtgaggt aaataaatta ccacgtagtg aatttagggc tttattacat
    27901 ttttgccaac atcctggaaa aattcaaaca cgtgaagatt tattgaaaaa aatgaccggg
    27961 cgggaactaa aacctcatga tagaaccgtt gatgttacta ttcgccgtat tcgtaaacat
    28021 tttgaagacc accccaatac cccagaaatt attgtcacca ttcacggtga aggctaccgt
    28081 ttctgtggta acatcgaacc ttaatcaaga taggagtaat tttatgaaaa aattaactat
    28141 tgtcgcatta acttgcttat tttctaccca tctattagcc gctcctgtcg gaaatacatt
    28201 tactggcatc ggtgtcggtg tagatgtaac tacaacaaaa tacaaagttg aaggagtaaa
    28261 aggcaaggat tccacaggtc cggcattagt tgtcgattat ggtattgagc aaggcaataa
    28321 ctttgtaggc gttgtacaag gaaaagcaaa agtaggtagt acaaaagtat ttagcgatgt
    28381 aaaacaaaaa aataaatata ctatagctta tcaacaaggt tatcgtgttg gatccgattt
    28441 attaccttat gtaaaagtag atgccagctc aagcaaaatc ggtaatgaaa cctatcgtgg
    28501 ctttggtttc ggtgctggtg caaaatatgc agtttcaagc gatgttgaat taggtgcaga
    28561 atacactcgc gataatttaa aacgtagcgg cacaaaatta aaaggtaacg ctttcaccgc
    28621 gaatgcaact tatcgtttct aatcttagcg gtaaagtttt ctgaaaaaat aaccgcttgt
    28681 aacacaaaag agcctgaagt gattcaggct ctttgccttt taatataata ctcgggctcg
    28741 aatcgtgcct tcgatcgctt ttaaacgttg taatgcttca tcaacattgt cgctctctac
    28801 atcaattaca acataaccga tagctgcatc ggtttgtaaa tattgagcgg caatattcac
    28861 atttgattcc acaaaaatct ggttaatttg atttaacata cctgggcgat tctcgtgaat
    28921 atgtaataaa cgtttggtat tagtatgaag cggtaaggat acttcaggga aattcactgc
    28981 cgtcacggtt gaaccgttat ctgaatattt cacaaattta ttggcaactt cggtgccgat
    29041 atttgcctga gcttcagagg ttgaaccacc aatatgtggc gttaaaatca cgttatcaaa
    29101 cttgcacaat ggtgacacga acgggtcatt aattgatgcc ggttcttccg gaaatacgtc
    29161 aatcgccgca ccacgcaatg agccgtcttc aatccgagcg gctaatgcgt ctaaatcgac
    29221 cactgtacca cgagcggcat taattaacac cgagccttct tttaattgag caatgcgttc
    29281 tgcactcatt aggtttttag tcgagtcgat ttccggaacg tgtagtgaaa tcatatcgca
    29341 ggttgaaagc aaatgctcta atgtcggcac ttgctgtgca ttaccgagtg gtagtttgtt
    29401 ttcaacatca tagaaataaa cctgcatacc aatggcttcg gcaagaatac taagctgtga
    29461 accgatatga ccatatccca caatacctaa tttcttgcca cgtacttcgt ttgcacctgc
    29521 tgccgattta ttccacaccc cacgatgcac ttccgcattt gcagtcggga ctttacgcat
    29581 taataaaatc atctctgcca gcactaattc tgccaccgag cgggtatttg agaacggtgc
    29641 attgaatacc ggaatacctc gtcttttcgc cgcattcaaa tcaacttggt ttgtaccgat
    29701 acagaaacaa ccaatcgcaa ttaatttcgg agcttgagcc agtacttctt cggttaaaaa
    29761 agtacgcgag cgaataccaa caaagtgtgc atctttaatt gcatcaatta attcttgacc
    29821 gtctaatgct tttttatgca cttcaatatt ggtataacca gccgctaaga gtacatcaat
    29881 tgcgttttga tgtacgccct caagcagaag gaatttgatc ttggatttat ccaaagaaat
    29941 tttagccatt ttttgctcct aagtatttgt atttgcaata ataaattatt ctaaaataac
    30001 tgcaccttca ggtgtgccga caatggttac attggcaaaa cgttgtgcaa aaataccgtt
    30061 agtcaccacg cctgcaatat tattaatggt atgttccatt ttcaacggct ccataatttt
    30121 aaagttatgt acatctaaaa tcacattgcc gttatcagtg accacgccct cacggtattc
    30181 cggcgaacca cctaaggcta cgagctgacg agccacataa gaacgagcca ttgggatcac
    30241 ctccaccggc agagggaaag ttgaacccag cacatcaact tgtttggatt tatccacaat
    30301 gcagataaat tttttcgcta atgaactcac gattttctca cgggtaagtg ctgccccgcc
    30361 acctttaatc atcgcccctt gtggcgtgat ttcatcagct ccgtcaatat acacatctaa
    30421 ttccgtcact tcattggcac tgaatacttc aatgccgata tcacgcaaac gctgttccga
    30481 ggctttagag gcagcaaccg ctcctttaat ctcatctttc attgaagcaa gggcatcaat
    30541 aaaacagttt acggttgaac cacttcccac cccaacaata gtgtccggtt ttacaaattt
    30601 taatgcagct tgtgccgcaa tttttttcat ttcttgttga tccattttta cctccacttg
    30661 tagggcaaat catcatttgc ccacttcggc aaggcgaaaa aaattttcgc cctactacat
    30721 ctattctaca catatatgta acaagcggtc atatttggca aattttttac caaaaacgac
    30781 cgcttgtcat tagttacgtt ttactgcttc aacaatctct tccgcacagc tttgagcaag
    30841 tacagcatct tcgcactcca ccatcactcg aattaatggc tcagtgcctg atttacgcaa
    30901 taaaatacgc cctttgcctg ccaagcgttt ttccacatct gccgctaccg ctttcacttc
    30961 gttactttca agcggatttg ccgccccttt ctcaaagcga acatttagca gaacttgcgg
    31021 gaacagaggc actgcttttg ccaaatcatt caggcttaat ttgtggcttg ccattgctgc
    31081 taatacttgc agtgaggcaa taataccatc accggtggtg tttttatcta acacgataat
    31141 atgcccggag ttttcgccac ccagtttcca gcctttttct ttaagctgct ctagtacata
    31201 gcggtcaccc acgttagcac gagagaatgg aatggctagc tcttttaaag ctaattccaa
    31261 gctcatatta ctcattaatg taccgactac accgccttgt aatttgcctg aacgtagagc
    31321 ttctcgggct aaaataaata gaatttggtc gccatcaact ttattgccta agtgatcgac
    31381 cataatcaaa cggtcgccat caccatcgta agctagacca acatccgcac ctgcttctac
    31441 caccactttt tgaagtgctt taatatccgt tgcaccacat ttttcgttga tgtttaaacc
    31501 gtccggctta gtaccgattt caagcacttc tgcccctagc tcacgcatta cattcggcgc
    31561 gatatggtag gttgcaccgt tggcacaatc caccacaatt ttatagcctt caaggcttag
    31621 ttcagacggg aaagtgcctt tacaaaactc gatataacgt cctgcggcat cattaatacg
    31681 actggcacga cctaaatttg ctgaatctac gcaatccata ggcttatcaa gcattgcttc
    31741 aatcgcttct tcaatttcat caggtagttt ttcaccaacc gacgagaaga atttaatgcc
    31801 gttatcgtaa taaggattgt gagaggcaga aatcacaatg ccggcttccg cacggaaagt
    31861 acgggttaaa taagcaattg ccggagtcgg catcggacca acgaatactg ctgataaacc
    31921 ggctgcggtt aaaccggctt ccagagccga ttccaacata taaccggaaa tacgggtatc
    31981 tttaccgatt aatacttttt tcgtgccttg ggttgccaac actttacctg ctgcccaacc
    32041 gagttttaat gcaaattcag gtgtaattgg aaatttacct acttcaccac gcacaccgtc
    32101 tgtgccgaaa tatttacgag ccattctaat tcctaagatt gataaaaatt tactttaaaa
    32161 aatattgata tgccggactt tcgctcacat tttggaagcg gtaacctagc tgttctaaat
    32221 gtcggtttaa ttctacttca tcgcctctgt ttaatgcaaa tgcggcaaga atatcgccat
    32281 aatcggctcc gtgagctcgg taatggaata acgagatatc ccagttagtc agcatttgta
    32341 agaattttag caatgcccct ttctgctctg gaaattcaaa gctatagagt tgctcatatt
    32401 gaatggagct tgctctaccg cctaccatat aacgaatatg ggttttggca atatcatcgt
    32461 ccgataaatc tgccacatca tagccgtttt gttgcagatc tttaataatc tctacttttt
    32521 ctgccgagcc tgaaattcgc acccccacaa aaatacaggc ttgtttatca tcggaatggc
    32581 gatagttaaa ttccgttacc gcacggttgc ctaaaatctc gcagaatttt aagaaactac
    32641 ctttctgttc agcaatggtg actgccaata aagcttcacg tttctccccg atttcacaac
    32701 gctctgacac aaaacgtaag gtgtggaaat tcacattcgc cccggaaaga atgcaagcta
    32761 agtttttatc ttgtaattga tgctctttca catatttttt caaaccggca agggaagttg
    32821 ccccggacgg ctcggaaatc gcacgcacat tttcaaaaat caacttcatt gaggcacaga
    32881 tttcatcatt atccaccaac accacatcat caacatattg ctgacacagg cggaaagttt
    32941 catcgccaat gcgttttacc gcaataccgt cagcaaaaag cccaacccgt tctaaatcgg
    33001 tcggttgccc ttgctttaag gcgtgatata agcaagcaga atctttagat tcgaccccaa
    33061 tcactttaat attcggcatc acctgcttaa ttaaaaccgc aatgccggca attaagccgc
    33121 caccgcccac tggaataaat acataatcta aatccggctt ttgttgcacc agttctaagc
    33181 cgattgtgcc ttgccctgca atcactgccg ggctgtcaaa cgggtgaatg aaggtcatac
    33241 ccaattcggt ggaaagctca atcgctttcg ccttagcttc atcaaaattc gccccataaa
    33301 gcaacacttc accaccatag ccacgtaccg catcaacctt aattgccggt gtgttttgcg
    33361 gcattacaat caaggctctt aaacctaaat gtttggcaga aagtgccacc ccttgtgcgt
    33421 ggttgcctgc tgaagccgca atgactccag cctctttttg tgctttggat aaatttgaga
    33481 tcatcgcaaa agcacctcgc aatttaaagc tgtgaaccgg ctgtcggtct tcacgtttga
    33541 taaaaataga gttgcctaac cgctcagaca gtttttccat tttctgtaaa ggggtcactt
    33601 gcgctaagtc ataaatattt gagcttaaaa tcgagcgtaa atagtcgata gcggattggg
    33661 aatgttgtgt ttcgttcatc attttgaaag gttatttatt gttagattga gataaagaca
    33721 ttggtaaatt tccaaggaaa attgaccgct tgtttggtta tttagcaatg gctttaacag
    33781 ggaatatttc ctctcgcccg tttgcgggag agagacagct taaaatttcg ttcagaaatt
    33841 ttaagcagag agaggggaaa ccttggatat ccccctctcc ctccgcaaat cttcgatttg
    33901 ctccaccctc tcccgcaagc ggggagaggg gaaatcacaa agttagtgat gaccaccgca
    33961 accacagcca ccgtgtccgt gatgatggtg atgatcgtgg tcgtgaccgc cgcagcaacc
    34021 atcgtgatcg tgatgatgat cgtgcccacc acaaccgcaa ccgtgttcgt gtccgccgtg
    34081 aacgtgtccg tgtgcgattt cttccgctgt cgcttcacga atgccaacca cttccacaga
    34141 gaaaagtaat tcctgaccgg ctaacatatg gttaccgtcc accacaactt catcgccatc
    34201 tacttcggta attaccaccg gtagcggacc taaatccgtg tccgcaataa aacgcatacc
    34261 cacttctaat tcatcgacgc ccataaatac atctttcggt acacgttgaa ccatattatc
    34321 gttgtactcg ccgtaacctt cttccggttt cacacgcact tcaaacacat cgcctacagc
    34381 tttgccttca agggcttttt ctaagccttc taccaagttg ttgtgaccgt gtaaatattc
    34441 taacggttgg tttaccggtg cttcatctac taatacgcct tcttgagtac gcacttgata
    34501 tgcaatgctt ggcactaaat cttttgtaat gttcatattg aatcctttaa ttcatcggtt
    34561 ttataaaata gcaagccatt gtaacgcttt attttatatt tgcaatctat ctggaaattt
    34621 taaccgcttg tcagtaaatt agtcgataat atcaggggtt acggtgtcta ccacatcaac
    34681 tgcggttccc accactccac caaccaccga agttaccaaa caaccactta ggaaaatagt
    34741 tgctgaaatc agcccacata ctttgattaa ttttttcatt ttaaaatcct ttcaaaaaca
    34801 aaaaacagcc ctaatcaaga ctagagctgc atcatattta ttcgcttaaa ttatttttct
    34861 tcagcgataa agcgataaac taccgcaccg ataattgcac cgacaatcgg agcaacccag
    34921 aacaaccata attgctcaat cgcccagctg ccttggaata acgctacacc ggttgaacgt
    34981 gccgggttta ctgaggtatt ggttaccgga atgctgatta agtggattaa ggttaaagcc
    35041 aaaccaatag caatcggagc gaaacctgct ggagcacgtt tatcggttgc acccataatg
    35101 atgattaaga agaatgccgt taataccact tcaattaata atgctgctgt taagctatag
    35161 ccgtgtggcg aatgctcacc ataaccgttg ccggcaaagc ctgctgtagc atcaaaagtc
    35221 ggaacacctg aagcaatggt gtataacact gcaccggcag caatcgcacc gattacttgg
    35281 gcaatgatat aaggcactaa atctttagcg ttaaaacgac cgccgactaa taaaccgatt
    35341 gaaaccgccg ggttgaagtg accacctgaa atatgaccga ctgcatacgc cattgttaat
    35401 acggttaaac cgaaggctaa cgaaacgcct gcataaccga ttcctaattc aggaatgcct
    35461 gctgctaaaa ctgcactgcc gcaaccacca aacactaacc aaaatgtccc gaaaaactca
    35521 gcaaaatatt ttttcatttt ttatccttac cctaaagggt gaatcacaca taagtgtatt
    35581 aactttccga atgctataaa acatagccaa ta
//
LOCUS       DANLRK010000004        34332 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.004, whole genome shotgun sequence.
ACCESSION   DANLRK010000004 DANLRK010000000
VERSION     DANLRK010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 34332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34332
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.004"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            46..264
                     /locus_tag="PXA33_000116"
     CDS             46..264
                     /locus_tag="PXA33_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL5119500.1"
                     /translation="MARNKRVRDMSNFEIRGELMKIGKSLSQLGIENGLAKTTVRNAL
                     DKPYPKGEQIIAKAIGKTPQEIWPSRYE"
     gene            274..2244
                     /locus_tag="PXA33_000117"
     CDS             274..2244
                     /locus_tag="PXA33_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HDL5119501.1"
                     /translation="MKEWFLAKEIAGLSGVPELPNSVSRLATKEGWQKRQIQGVRGVT
                     YEYHLTSLPIETQQQLRLNAALAVIPQAAELQPKRDDPALIARLNNATDKGRDKAKGK
                     AEACMQLQAFLDQGFSYTQAEAGAATAKNVSQGSLKNWYYKVKGHPVHLWQAILISES
                     GRSKKPQLKAKITEEAWDCFLADYLRPEKPDLRASYRRTQAIAKQYGWQIASLQTFQR
                     RVLAEVPYEVILLKREGANAVAKLVPALQRTVKDILAGEWINGDGYQHNVFVKWHTGE
                     IVRPKTWFWQDVRTRKILGYRTSISENTDSIRHALMDVIFNVGIPKTLTLDNTRAAAN
                     KAMTGGIENRYRFKHTELDPKGIMPILGIDVHFTSILYGEGHGQAKPIERAFGRGGIG
                     EKIDKRPELSGFYTGRNAQETPDNYNGGKDGVDYNTFLKAIAAGIEEYNSQTERHTEM
                     CRGELSFNQVWERDYHPSNVRQASPEQLRLLFLQAETVSIKRNGSFTLKAAGKLYGLT
                     NVYWAESLIGITDKKVVARFDPDDLHGNVYVYNLEGQFLAEAVCREAKGFGDTSASRE
                     QGRLYQKVVKSAKAQAEALELLEAHELASLAPQVDVPEPIEKKVKEVLVKEDFIVDFN
                     TVRKATVVEETEEISIFDEVLLKQEKQLRIVE"
     gene            complement(2424..2912)
                     /locus_tag="PXA33_000118"
     CDS             complement(2424..2912)
                     /locus_tag="PXA33_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5119502.1"
                     /translation="MLFRSLTDDEIHQIALDSARATFSGFKLKRANAIVMKNCITLFK
                     LSTINFCLAEYHSSFHHLTSIDDLDGIMPTVKKVAQMLSLQESKALIWLLYLKWLFVM
                     RQRSLQVLLENSADLIQGEVIAQPLPTFVKAVLAFQLETYHGLPEWIPEFDSAEFWQS
                     LS"
     gene            2944..3885
                     /locus_tag="PXA33_000119"
     CDS             2944..3885
                     /locus_tag="PXA33_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL5119503.1"
                     /translation="MTLIDQIKQHIAESGSSQGKVAKEAGINAGALSAYLNENYKGNN
                     EELEAKLIAYLERIEAKKREFVEAPSFIETKTANQIFGSLRFAQNTGVLAIIHGASGV
                     GKTQAAREYRKRYANVWLVTASPSRSSLSEVLYEIALELGMNDAPRRKGTLARLIVRK
                     MKGTAGLLIIDEADHLPYEALEELRLLQEEVVNESDIGAGVGLVLIGNDKVYTRMKGG
                     INPAHEYGRLWSRSAKRTSIQKTKKEDTQAVAKAWGLSENQEALKVMQAITETGGGLR
                     ILTHTLRLAAMVAKDRMIPLTADLIQLARKDLLSTGD"
     gene            3888..4202
                     /locus_tag="PXA33_000120"
     CDS             3888..4202
                     /locus_tag="PXA33_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5119504.1"
                     /translation="MRTPKPKHSFNRTNQAACRFLKQTQKAIIRLNSLGFTVLNIDFT
                     RIKPRIEVEIGNNKHIAQGLIYEGKAYRYSFGKSEDLGRWEGYYTMLEGIRVWWRQAA
                     IH"
     gene            4214..4450
                     /locus_tag="PXA33_000121"
     CDS             4214..4450
                     /locus_tag="PXA33_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5119505.1"
                     /translation="MKTLALLTALLGLVGCDLVQSKQAKNYIGNNLTEICIDNVVYLI
                     YSGDKKGGITPKVNKDFYPYTCTNKEERQNGKTR"
     gene            4434..4727
                     /locus_tag="PXA33_000122"
     CDS             4434..4727
                     /locus_tag="PXA33_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5119506.1"
                     /translation="MAKPDETPITKAECQSQLAELGVQYKKLPMAITRHICNATTNIH
                     GKVIKVSVVERVGYGVQITAQGNEKSCLVTYEAMLGMAEAMGLFDEVKEQNND"
     gene            4849..5067
                     /locus_tag="PXA33_000123"
     CDS             4849..5067
                     /locus_tag="PXA33_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005606434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HDL5119507.1"
                     /translation="MNDELAAISASYAQHNRYQHYAEKAAEAERKGNYKEAQDHWEVA
                     KLSAKKTANRDWAEQRAEFCKRMHNKPF"
     gene            5077..5259
                     /locus_tag="PXA33_000124"
     CDS             5077..5259
                     /locus_tag="PXA33_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005822929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5119508.1"
                     /translation="MTETVKAQLNSQLNEAIIQLIQAQKYLNQDDAIRSGVYIGTVQD
                     LLPKVHLKLLTANRKH"
     gene            5276..5665
                     /locus_tag="PXA33_000125"
     CDS             5276..5665
                     /locus_tag="PXA33_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5119509.1"
                     /translation="MAITEEQWKEIKQKLDSIIGRVKFRYKEHLLTVDVVQIKRSLKL
                     AVYVDGEIDGAWTKEGHEIRPYLEEVWYRKERPFFNAKEKKEYRGLMSKKKLNEKIVT
                     YSPMFPSPTALTRQYKKLDGLELIEVI"
     gene            5714..6001
                     /locus_tag="PXA33_000126"
     CDS             5714..6001
                     /locus_tag="PXA33_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5119510.1"
                     /translation="MNKNEFFKKYHLEGSLAILDLDANDDQSQFCYQALLEYRELPLD
                     TFLAEYSDDEDMTESEFIELMLDELEWIIRRAKNAKRFVKKRQKTMREEIM"
     gene            6120..6665
                     /locus_tag="PXA33_000127"
     CDS             6120..6665
                     /locus_tag="PXA33_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1018 domain-containing protein"
                     /protein_id="HDL5119511.1"
                     /translation="MTDKAKLIQLIHIAKSQLAMDDLSYREMLKRLTNKTSSTKCTVV
                     ELHKVLHELQTKGAKVKYFAKRSKKPTAYSPATGETAVKSQIAHKIRAVWITMGKHGF
                     LNDPSEKALNAYMRKVINNKTRPTILLNVSALEAGDASYMLEILKKWHKRVMIKALSK
                     SCTVDPKISYDELVEWYNEVM"
     gene            6652..7185
                     /locus_tag="PXA33_000128"
     CDS             6652..7185
                     /locus_tag="PXA33_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005693508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5119512.1"
                     /translation="MKLCRCPICHSDIHLEALVEDEAGRELLGKISQLTHGVAQPMVA
                     YLGLFKPQKSNLNNSRALKILNDVLALYPCSLLLAQALSETVVSIRKKRQQAMENGQK
                     IEPLPNHNYLKSVYETQKVHFAVVRTGKTERETMKAELEKTRNAIAYVQRFVDLGREE
                     VVKNSPEYQIWLAHKPK"
     gene            7350..7709
                     /locus_tag="PXA33_000129"
     CDS             7350..7709
                     /locus_tag="PXA33_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL5119513.1"
                     /translation="MELVDIFEEKAPEVLTDLAKHIEVALEEKCQFSPEKAKQIGIDI
                     AQQMAQNWGGEVIYIPRNLLMALNERDMKIYEEFNGNNHRELARKYNVSMQWVYKIVK
                     KVHKDEVARRQMSMFEM"
     gene            7845..8354
                     /locus_tag="PXA33_000130"
     CDS             7845..8354
                     /locus_tag="PXA33_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 108 protein"
                     /protein_id="HDL5119514.1"
                     /translation="MSQNLTFRQIFDRLIGHEGGYVNHPQDPGGETHWGITKRTAVAN
                     GYMYAMKNMTREQAYQIYEKAFWQRYRCAELKPAVAYQFFDAVVNHGFGNASRMLQRA
                     VGVADDGIIGNITLQAINAKSESDLLMLFNAERLKFYTKLRNFPTFGKGWVNRVAGNL
                     EYAAQDNEV"
     gene            8357..8626
                     /locus_tag="PXA33_000131"
     CDS             8357..8626
                     /locus_tag="PXA33_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2644 domain-containing protein"
                     /protein_id="HDL5119515.1"
                     /translation="MAKLFKELLSTNGTLSTTSTVQFFGTLCVFGLMVYAVITQQPYA
                     ESLLNNVLIYLFGATTAKGVVTSYQAKIKGGIHKQQSTFKEEEGC"
     gene            8620..8967
                     /locus_tag="PXA33_000132"
     CDS             8620..8967
                     /locus_tag="PXA33_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2681 domain-containing protein"
                     /protein_id="HDL5119516.1"
                     /translation="MLMQQILGVIGVIAAILSYALFKSWQLKRERKKNAILQAEKQQQ
                     AVEIEQKKAEVKYVYISKKNNEKVTRSSASDIDDKLQQHGYFRDNNRLHGVQSDLSEP
                     CGYAGNETSDSCS"
     gene            8994..9146
                     /locus_tag="PXA33_000133"
     CDS             8994..9146
                     /locus_tag="PXA33_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5119517.1"
                     /translation="MKYIKKLRKRWQIWRFLKNHPEVAQRRDLLAIAIKQGVENPVGR
                     ARRREP"
     gene            9146..9481
                     /locus_tag="PXA33_000134"
     CDS             9146..9481
                     /locus_tag="PXA33_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2730 domain-containing protein"
                     /protein_id="HDL5119518.1"
                     /translation="MDLFTVFWESIRANFGFISALLTIVVTAFWLKLDSKYAKKNDLG
                     ELLEIAKSHDNRLTTLETKVENLPSAVDVEKLKTLVTDVKGDTKATSKQVDSISHQLG
                     LLLEAKLKE"
     gene            9483..9779
                     /locus_tag="PXA33_000135"
     CDS             9483..9779
                     /locus_tag="PXA33_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5119519.1"
                     /translation="MSLRDLLTQDQRLVILRSLAEAGYDANESIISDCLDLYGHDISR
                     DLVRTHLSWLEEQGLLTLERLRDGYMVASITQRGLDVAQGRVVVSGVKRPRPKI"
     gene            9802..10374
                     /locus_tag="PXA33_000136"
     CDS             9802..10374
                     /locus_tag="PXA33_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3486 family protein"
                     /protein_id="HDL5119520.1"
                     /translation="MSEKNTRGRASKVDLLPPNIKTQLAMMLRDKQYSQSQILEEIND
                     LIRDCGLPDEMLLSKTGLNRYASRMERLGAKIRNAREVAEIWTKQFGEMPQTDIGKTL
                     MEMVKQIAFETSLKLGEQEGGIEPKQLALLSSAIQRLEQAESLSFKREQAIRKEVAQQ
                     AAETAEKVVVQAGLSAETVRTIKEQILGIA"
     gene            10374..11930
                     /locus_tag="PXA33_000137"
     CDS             10374..11930
                     /locus_tag="PXA33_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HDL5119521.1"
                     /translation="MALLNNRPLNELAPECRSFLESIHAFNPNELLLGYQKRWIADDS
                     QLKIAEKTRRCGLTWAEAADNALIASTRKSDGGSDVFYIGSNKEMAREYIDAVAMWAK
                     AFNRVAGEIQEEIFEDEDKDILTYVIYFSSGFKVKALSSNPKNLRGMQGVVVIDEAAF
                     HEYLAEVLKAALALTMWGAKVRIISTHNGSENLFNELITDSRAGRKRYSVHTITIEDA
                     CRDGLYQRICQVVTKKTWSPEAEKEWIENLLNDTASEEDALEEYFCVPKFGSGLWLSR
                     ALIERQMSEATPVIRFTAKDGFSLVAEPTRYAEIAEWCELELEPILATLNPDLLHFMG
                     EDFARSGDMTSLVVLAQQQNLVRKVAFIVELGNMPYKQQEQIVMFMLKRLPHFAGGAF
                     DARGNGGYLAEAARDEFSTLIDCVQLSEKWYREHTAPFKAALEDGELEAIPKDADILA
                     DLRSFQVVKGVPRIPDKRTKSADGKSKRHGDTAISLLLAHYASRQLVQMPVEPVSRRP
                     RHSQQLTEGY"
     gene            12049..13503
                     /locus_tag="PXA33_000138"
     CDS             12049..13503
                     /locus_tag="PXA33_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 family protein"
                     /protein_id="HDL5119522.1"
                     /translation="MAKKNNLISEIATRARSIDYWAFGYYLPNPDPILKRMGKDISVY
                     RELLSDGQVRSGVRRRKAAIKGLEWRITTTNNEKVDEQIYQIFNRLPMNNLITEMLNA
                     ALYGYHISEVIWAKEGELFVPTEIIGKKPEWFMFDDENLLRFRTKENWADGELLPDDK
                     FLLTTQEATQDNPYGLGDLSLCFWAATFKKGGFKYWLEFTEKYGSPWLIGKHPRQAHN
                     TDKERLADALEGMISSAIAVIPEDSSVEIKDAAGKSASTDAFEKFLNFCKAEINIALL
                     GQNQTTEQESNRASAQVGLEVMEDIRSDDQAMIEATFNTLLQWIVKYNFNVEQLPKFE
                     FYEQEAINTDQVERDSKLHAMGVRFSKAYFEREYGFEAGDIEVQAVEKAEKSANTTEF
                     SEGQRRTQVPMPVPILEQLEHEGEAMVDDWLQGVRDKLAQADSLEDFQSQLDSLIPEL
                     SFAEYGELLALGSTVAELAGRQSVNDEIKAKRNA"
     gene            13496..14773
                     /locus_tag="PXA33_000139"
     CDS             13496..14773
                     /locus_tag="PXA33_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HDL5119523.1"
                     /translation="MRKFTFKQQAQYFEKKLNLRTNSYLDILGEEHDYFFVVAGANRN
                     EVLSEFRKAVDEAIHNGETLENFRKRFDEVVAKTGWEYNGGRNWRTRIIYDTNVYGAY
                     NRGRLQQHLELAEDMPYWEYQHNDNAHPRPQHVAWDGLVLRYDDPFWKYHYPIKAYGC
                     HCTVQALDDFDLKHDGKTVSPPPEIEFEQKTVGVRTGNSRVVTVPKGVDVGFTPHNFD
                     NLKAGRAANVDKLLMQKMVTADPIFASKAVSDLLAKEPVAKLHNQAFKNWVNDVANAK
                     GSALSQVENMQLLGVLPVAVIERLQQINLAPQSAIIAITKGDMVHALRELKQNTGIAV
                     PLEFWEKLPAKLQYPNAILLEKQQAETTLLFIYDVAQTKIAVKVNYDATLQDRHGAKG
                     RQKVLLNRAVTGSLFQDTTGLKSSKYEVLLGEM"
     gene            complement(14975..15319)
                     /locus_tag="PXA33_000140"
     CDS             complement(14975..15319)
                     /locus_tag="PXA33_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5119524.1"
                     /translation="MMVNAEQAIELVYDLLLSRQWLVTKAEKLPLDPLSEKEAVMFLY
                     TLDQQTEASWLQLTPEQRATANGLIMDFIAKCLTSTKQWLVSDNIVPELQAIEIIKHE
                     IFLSHNSLVMPN"
     gene            15383..15847
                     /locus_tag="PXA33_000141"
     CDS             15383..15847
                     /locus_tag="PXA33_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HDL5119525.1"
                     /translation="MIKITLNNTQAVKSLQQIANQLEQPRRLYGILGETLKKIHTARF
                     KAEQDPDGNNWKPLASSTLALKRKRGKSTKILRQDGYLADKTAYNVTSDNVEFGSQEV
                     YARLHQFGGKAGRGKKVTVPARPWLGVGDKDEQLLLRKAEWHLGQIIGRMGK"
     gene            16082..17197
                     /locus_tag="PXA33_000142"
     CDS             16082..17197
                     /locus_tag="PXA33_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HDL5119526.1"
                     /translation="MTQIEIFKAGKRYDANGTLIDITPEMLQQTVAAYNPEFHEAPLV
                     IGHPKSNNPAWGWVKALSLEGDVLKAEVDQLDAEFSEMVASGKFKKVSAAFYLPDSPN
                     NPHKGVLSLRHVGFLGAMPPAVKGLKQVEFAEDDDFVVFSEWGVASLFSRFRDWLVSE
                     FGLEKADKALPPHEIDWLKEEAMRSQISKQVHAEQATPEPIFNEPAQPEGEPEMSAEE
                     KEELERLKAENEQLKAEKAQAEAEKMEAELEAEKAGNAEFCEGLVKQGKLAPVAKAAF
                     VQALNNLSVLKAGREPEFNEGEDVVSQFKSAMSQSPQIIQFGEYATADKANPQEAEAV
                     EYAEGTDPVSIEADQKVRAYMKAHNVGYAAAFNAIYR"
     gene            17228..18154
                     /locus_tag="PXA33_000143"
     CDS             17228..18154
                     /locus_tag="PXA33_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007524268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HDL5119527.1"
                     /translation="MTTHNLQKLRVQDPVLTELAQGYHNNELVAETLMPTVEIEKEAG
                     KIPTFGRLAFRLPSTVRNLRGSSNRLDPEDIGAIDVALEEHDVEYAIDYREENEAIFS
                     LRQFALNTTQDVIALGREKAVATLALDESRYETDNKVTLSGTSQFSHKDADVFKVFDT
                     AIRAVKRAIGRKPNVCVISGDVWAALKEHPAVVEKLKYSQVAIVTPEVFAKLIGIETV
                     KIGEAVYEESGQLKDIWSKDIVVAYVAPRSTGGKGTVYEPSYGYTVRRQKGLFVDTYK
                     ESGGKIEVVRTTDIHKPHLVGAAAGYLIKAAV"
     gene            18223..18504
                     /locus_tag="PXA33_000144"
     CDS             18223..18504
                     /locus_tag="PXA33_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5119528.1"
                     /translation="MAKFIVLHTPILHDGKRYEVGAEIELTAEQAENNAINLKAVEAV
                     PKPPKNASKPEDEPKPEGEPKPEDAVTEDDVKLAEAKIAEAEQPKGNKK"
     gene            18504..18938
                     /locus_tag="PXA33_000145"
     CDS             18504..18938
                     /locus_tag="PXA33_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1320 domain-containing protein"
                     /protein_id="HDL5119529.1"
                     /translation="MYVQIDELLTAFSRKIVVQLSNDEPTATEPDLKMLETAVKVANE
                     RIDAALRSRYTLPLADVPTLINQHALTLARYWLYARRPETKMPDTVKETYQQAVKELE
                     QIATGKLHLGIAESSQILEDDVLADNSEYEVRANARIDTSRY"
     gene            18944..19441
                     /locus_tag="PXA33_000146"
     CDS             18944..19441
                     /locus_tag="PXA33_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gp37 family protein"
                     /protein_id="HDL5119530.1"
                     /translation="MSATQPILEDICQHLRQALPEWEVELFPNDPSTYHLSHINGAVL
                     ISYLASKFEKPRTTQAVLQVRHVQIALTVLTRDLHNDVGALDLLDKLRLLVVGFKPQN
                     CGECWLIDEFFNGSSEETGLWQYQLILQTETQQVQHQAVENLPKFVTAHLRRQDQPGS
                     PGLKT"
     gene            19526..20911
                     /locus_tag="PXA33_000147"
     CDS             19526..20911
                     /locus_tag="PXA33_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HDL5119531.1"
                     /translation="MTFHHGSETERVNGGSVPVSVVDSAIIGIVGTAPIGAVNELTVC
                     LTKKDFAKFGTILNQGFTLPDAFDILARYAAGKVYVVNVLDPSRHRTTVTDEVLTLDR
                     NTLTAQTAKTGIIAVTRVKDGANVLNADRYSVDLQTGVVTFVSAPSAPKISYIYADPS
                     KVTEEDIKGGVDSLTGKRKGFELLRDGFNLYGADAKILICPEFDKTASCAAALATLAD
                     QLHAKAYIQLPKGTSLSKAIQGRGPAGTINASASNENVRHFFPYALGSSNTLESLATH
                     AAGLRMLIDVEKGYWFSTSNKELKGVIGLEIPLTARVDDKQSETNLLNAVGITTIFNS
                     FGTGFRLWGNRSSNFPTVTHISNFEVASRTGDIIDESIRQAELQFIDLPIDDALLDSF
                     LETIDTFLRTQKSLVGYSVSLDYEYDLVDAFSRGQVPIVYEYTPKIPGERISNKSVMT
                     RKYLANLVSQR"
     gene            20922..21437
                     /locus_tag="PXA33_000148"
     CDS             20922..21437
                     /locus_tag="PXA33_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HDL5119532.1"
                     /translation="MSIAINQIVNGNVYINGNSQMGRVNEVKIPDIEFEFIEHKGLGM
                     HGVIKLPAGTNAIEAEITWDSFYPEVRAVMLNPYKYTQLQIRSNLQVFNSQGLAAEEP
                     MVTIMNVSAGKIGGTGHKNKENAEFADTLNVHSIKQTVAGRELLFIDVLANIYRVNGV
                     DVLQKFRTNIG"
     gene            21533..21847
                     /locus_tag="PXA33_000149"
     CDS             21533..21847
                     /locus_tag="PXA33_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HDL5119533.1"
                     /translation="MANAAQQVLTNLRVQTTYKLQYPIALPDGRVIETLDVRRPKGKD
                     FRLLDEKGFDPEKDGVKILRFYIQQLTTLVPEDIDELDAADIKGLNNLLEDMLIEGKS
                     DK"
     gene            21844..21996
                     /locus_tag="PXA33_000150"
     CDS             21844..21996
                     /locus_tag="PXA33_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HDL5119534.1"
                     /translation="MSELDTVYADLAWWFKFSPSELLELDLVDIPKWVEQMNRQIKAG
                     YGQILR"
     gene            complement(21975..22277)
                     /locus_tag="PXA33_000151"
     CDS             complement(21975..22277)
                     /locus_tag="PXA33_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5119535.1"
                     /translation="MLTKLNNRITEWAGFEACVTRTECTAKWLLAVALVGWSIFYWLD
                     AIQSIQSLSEGVSFVLIYGLILTILAWVGVYLAWGVAILLTLATEPFIWLYRKICP"
     gene            22325..24982
                     /locus_tag="PXA33_000152"
     CDS             22325..24982
                     /locus_tag="PXA33_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDL5119536.1"
                     /translation="MANNLAIGMVIGASLSSGFHGAFSGAKKKLEQLEQAVAKNQQSH
                     SKLGSELTALRSKQATLYAEMSRASSKGGAGLALMQQEYKKIGKEIANISREQRKWST
                     ELEKSIAKQTKLRNAMERHTQAKENRDELKGKIIGTVASAAMGVGVMKTYMEQEEAAN
                     NLKISMLKADGSFGKFEEIGKIADTLGTDLPGTKKDFYKLAMALKKQGVSDDVLTGGA
                     LKTSAELNVLLDMDQESGGEFLAKFMESHRLNENELPQAADYLQRAMFAGGLSKEQMY
                     ESMKYYAPKLNSMKLTGAENTEKVLAIEAMAGQQGLEGSTFGTGLNMMLSRMNKGPEM
                     LKAAAKGMKAEAREMMEAVGVEFNFWDKNGSFKGIDGMLAEMTKFEKIRKKFGDKGVG
                     LVAEELFGIEGGRLADILAMKGQKGLDEMLSKMREQASLQDRINLKTNTLGAALESLG
                     GAWETFVGDIGSVFAEDIKGFSKGLQSAVEWLVPFVSKHKEAIKFVGGLVAGLVILRG
                     VLQAGSFVIQTVTMAMNALKIAAMTNPIVLVIGAIAAGAYLIYSHWDSIAPWFANLWQ
                     NVTAYFNQFWTWVKGVWSGVTDWVSSVWNGVATFFGQLWTTITSLFSVAWTNIQTIWG
                     GATAWFSGLWDGIKSAAFGMFDSISQKVSSVVASIKSFLGFGDDVDKMGAKIGNVATQ
                     VISKEHSGQLSKTADMAANTGFSSGGFTGQGGKYTPAGIVHKGEYVMTKEATSRLGVG
                     MLDSLNYGGAMGFTQPVLQPILGKQKGFFGSLWDDVKFGANFVGNLLGLNQGSLKTPD
                     FNPNATNASGQNSSIFSNYEPLNRNAVTQQESSQAPQIVVNFNPTINVNGNATQEAQS
                     QIMQQLQMSAYEFEKLLNRVLDQRSRRAY"
     gene            24992..25915
                     /locus_tag="PXA33_000153"
     CDS             24992..25915
                     /locus_tag="PXA33_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL5119537.1"
                     /translation="MYFMLGNIAFEPVNLTEFSEQHTAEFAEHAVLKGKPRLQAMGEK
                     LNELNFSLRLHHTIGGVESRYQALLEAKAKQAALALIWGSRYKGDYVIVDISSTTLFT
                     DGRGNALAREMQISLKEFVGDNDGGVLGEALNFGGGSLLGSILPSGAVTTLSQIKTAV
                     SRGVELYNSGKRLVDEVQNTVAIIRQLKNDPATALAYLPAALANLDGALGHFGNLTGS
                     SATLGGLQEKLPAAVVFSEEIGEIYQSLQTMRSSLGNASANSWDNWFTPAESALTEVT
                     DSFDHLAKPVAKMTAWIVLRTDEEAENDTVA"
     gene            25899..26126
                     /locus_tag="PXA33_000154"
     CDS             25899..26126
                     /locus_tag="PXA33_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HDL5119538.1"
                     /translation="MTQLLKHIVKQGERWDNLSYQYYGNAFEYGRIIDANPHIGFCEV
                     LPIGVTIYIPVLNVKPTNNENMPPWLRGRDA"
     gene            26119..27183
                     /locus_tag="PXA33_000155"
     CDS             26119..27183
                     /locus_tag="PXA33_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5119539.1"
                     /translation="MRNVPKPDFSLFYEKTNITAEIEPHLVQLTYIDHLEGQSDELTV
                     EFEDIQGKWIRQWFPTQGDKLKAAIGYQGEFLTDIGEFEIDEVEYSYKPSTINLKALS
                     TGISKANRTLKPKAYENTTLAQVVAKVADNLKLKVVGKIKAIPIKRITQYQERDVEFL
                     ARLAREYHHSFKIVGNQLVFTDKSELGQSEPVLMLEERDTISLRLRDRIKDTAKAVEI
                     SGYDTSGKKVVKKRKKAKALRPNIKQAKSASEDTLKIITRGESQEQIDARGEAALATQ
                     NEDQAAGDIELIGNPKLVAGATILLRNFGVFSGKYLIKSSRHTISRSGGYTTHIEVRM
                     LEFIADDLITLGMEAGNANA"
     gene            27170..27706
                     /locus_tag="PXA33_000156"
     CDS             27170..27706
                     /locus_tag="PXA33_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HDL5119540.1"
                     /translation="MQTHNFGATYQEGIVSAVDPKTHKVRCKIPALEDLETAWLSFLT
                     PNAGGNQFYCLPDVGELVAMLLDAQGEGGCVFGAIYNEQDPTPVADSDMWVKKFSNGT
                     EISHNRKTGDVVVKTNGHLTATAQGGATINANTVVNGTLHATGKITSDEEVSAPVVKQ
                     GTVELGTHKHGGSPEPNK"
     gene            27761..28123
                     /locus_tag="PXA33_000157"
     CDS             27761..28123
                     /locus_tag="PXA33_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HDL5119541.1"
                     /translation="MNRQTSIQLTHWQLAPSTDTVEVVQGIDDIHLCIVNILSTQKGT
                     DILRPEFGSDHFRYIDYPEDVAIPNFVREITTALQKWEKRIEVEEVLVDGSAPHFIFT
                     VMWHLTDDVYREIYRTTV"
     gene            28133..29236
                     /locus_tag="PXA33_000158"
     CDS             28133..29236
                     /locus_tag="PXA33_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HDL5119542.1"
                     /translation="MTEAIKIIDDDVKTVLAEAIADYEKRTGKILQPAHIERSIIQTY
                     AYREQLLRKGINHAFLQTFPQFATGIALDLCGETFGCYRLRDKPARTLLRFSVSGEHT
                     AIVIPKGTQVAINDELYFSTLNDDVITPLITYVEIDAECNQVGVIGNGWESGRVSKLK
                     TALATNYKVTVENIDITSGGLMQEEDEPYRKRILAAPEAFSNCGSIAAYDYHVRSVSQ
                     DIADVNISTPRGGLVRIAVLTQKGLPDTRLLDDIKKHVSAERLRPLCDMVEVIKPTER
                     RYQITARLILLDGYREDLVKAKAREALQLYLSDKTKKLGLDIVPSAIISALRVEGVYD
                     VNLIQPVKTIINETEWANCTAISVEVEEERRDG"
     gene            29229..29795
                     /locus_tag="PXA33_000159"
     CDS             29229..29795
                     /locus_tag="PXA33_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL5119543.1"
                     /translation="MASLHYADIIVRDPKYKALADLSKRLNLLDTSQIMTTLVDLLGD
                     ECIPLLAEKWSVTGYDGLLVADSENSKRALIKRAIELHQLKGTPWSIREVLRRLGFGE
                     VEIDEGLKNRLYESTYVSGIPENERWAHYAIRLSNPITNEQARNIRKVLRNFAPARCS
                     LAVLDYKADPIRYNNKATYNGTYNHGSS"
     gene            29805..32084
                     /locus_tag="PXA33_000160"
     CDS             29805..32084
                     /locus_tag="PXA33_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HDL5119544.1"
                     /translation="MAGVQETAQWENHVYRIEENDPVHGGENGVTNKPIKHLANRTLY
                     LRRLLTEAGQRINPKKITATSKNSNDLTGHTHEIDHASLTQKGIVQLNASIQSTSTTE
                     SATPSAVKAAYDKAATAEQKATLANEKVEAIKVGGRNLLIQSNTPHNGNAYSVRYALA
                     EAPKVGEEIVVTLWGELGSQRTGIGVYNSQGATELAKLVKIRDGVYQGKGIWRKPMSE
                     GRERTPNDTHLNVYFYPKTATDNNRIERIQLERGSIGTDWTPNEADLKAEISAIRTKL
                     IPVRGGNVATTLNAAKRSDWFREMQIPYQSGWGYYTQAFHNSGTQHNYSNLPLTGRYY
                     FIAELYHNSGYSHLRITYPSLKRTFESAINLNNEDLILDWREVVFLKDGLYQGTFKTT
                     GTLESAYRLRAARDDERYQPYLQMVDMGYDLANPPVGKNKIIGSVDYYVKNGTSESSK
                     ATMMAAAMPDKNVFWEVALWNAAQQRNVLFKGYSKTNNLSIGKTTDNERDRVQVNGTI
                     QATAPADNANNDQVPTTAWVRKMAKILADGKVSKAGDTMTGHLFIKNGEYSAVHTYNT
                     SGWFAKWEAAPLSDSHFAAIVYANDRETVINRVLIPKKNGTVALINDIADTVRAAVSR
                     SDKSRSRTFPLQTGKKYTTTGSVTIYPDGRIVQIFHLKNIKAAWFDYEASAVGDRHRV
                     VNIPLWTAMPNKIFDVDVKTVRPSNNASTYYIEAAEWLSAWQIYGNDTNKSSVNINLS
                     RFRGGKDEDMDLYVTVEGY"
     gene            32085..32687
                     /locus_tag="PXA33_000161"
     CDS             32085..32687
                     /locus_tag="PXA33_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5119545.1"
                     /translation="MAYFVNVIDEQGNYELIDDEFIGLYPDLDFNTLPKLTDKQYQTY
                     LAKANGKERFVNGEFVYEQIKVDMQAVISAEKSAKLAEINQKAQAFINDLAKYNETPK
                     FERDTWLEQAKEAKAWVADPTVQTPTLELIAQMRGIPLDTLRQKAYEKAMAYQTVAAI
                     VAGQRQGYEDRLEQAETLEQIQAIEPMYQLPQGANNEQTD"
     gene            32671..32946
                     /locus_tag="PXA33_000162"
     CDS             32671..32946
                     /locus_tag="PXA33_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5119546.1"
                     /translation="MSKLTKLKSWSYHVLIAIDQLFNALTGGAADETFSSRCYRGAVL
                     AENPKKRWRFWHSFLNCLFLDPKHCYAAYKAEVHRKQYPKAFRLGGI"
     gene            32946..33233
                     /locus_tag="PXA33_000163"
     CDS             32946..33233
                     /locus_tag="PXA33_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5119547.1"
                     /translation="MKKQNLKLVRGDDVSFRFSIKDSTGQPLALKDLWFDLHLVSYQG
                     EVLIALSTDDGSIETAEAGQMVLHFSHQQTARAEWTSANYDLQLTHDYTRV"
     gene            33243..33407
                     /locus_tag="PXA33_000164"
     CDS             33243..33407
                     /locus_tag="PXA33_000164"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5119548.1"
                     /translation="MTSIIELDLTDDLIIEVQLDSTIITGDSFLANLPDLLQIYNQAK
                     SDSSKELNND"
     gene            33400..34104
                     /locus_tag="PXA33_000165"
     CDS             33400..34104
                     /locus_tag="PXA33_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5119549.1"
                     /translation="MTKHKNHENHPIAITFYQLGKDVGDINRRLAVIEQREQSAQPSQ
                     PQAEAVIMPQQDAVAINNINAVLEAVEKYKTFEKYNTQWQERFEKFLSRNPNDYELSE
                     EQRQKLYHTMCQHLWQVNFTEINSKQYELPIPQQFQGKFGYIVKTSGGSTEISFELLS
                     ETHSTYLYPDSYIPEGGGDVMLKPAYIYLVIPDLVAMREPEVVGVSPLKLNILQKHLL
                     TPQKLQEQKPLNTKAN"
BASE COUNT        10829 a         6859 c         8107 g         8537 t
ORIGIN      
        1 atcatttaag tagtttctaa aactaacttt caaggatctc acaaaatggc aagaaataaa
       61 agagttcgag atatgagtaa tttcgaaatt cgtggtgagc tgatgaaaat cggtaagtcc
      121 ttatctcagt taggtattga gaatggatta gcaaaaacca cagttcgtaa tgcgttagat
      181 aagccatatc ccaaagggga gcaaattatt gcaaaagcga ttggtaaaac accgcaggaa
      241 atttggccct ctcgttacga ataaggagct tttatgaagg aatggttttt agccaaagaa
      301 atcgcaggat tatctggagt tccagaatta ccgaatagcg tttcaagatt ggcaaccaaa
      361 gaaggatggc aaaaacgaca aattcaaggt gtaagaggtg ttacttacga ataccacctc
      421 accagcctac ccattgaaac ccaacaacaa cttagactaa acgccgccct tgcggtaata
      481 ccgcaagcgg cagaactaca acccaaacga gacgaccccg cacttatcgc aaggctcaat
      541 aatgccaccg ataaaggcag ggataaagca aaaggcaagg cagaagcctg tatgcagttg
      601 caagcctttc tcgaccaagg gtttagttat acgcaggcag aagcgggagc ggcaaccgct
      661 aaaaatgtgt cgcaaggttc tttaaaaaat tggtactaca aagtgaaagg tcaccccgtc
      721 catttatggc aggcgatctt aatttccgaa agcggtagga gtaaaaagcc gcagctgaaa
      781 gccaagatta ctgaagaagc gtgggactgc tttttggcgg attacctccg ccctgaaaag
      841 ccggatttac gggcaagtta ccgccgaacc caagccatcg ccaagcaata cggctggcaa
      901 atagcaagtt tacagacttt ccaacgccga gtgttggcgg aagtgcctta cgaggtgatt
      961 ttgctcaaac gggaaggagc aaatgcggtt gccaagttag tgccggcact acaacgcacg
     1021 gtgaaagata ttctcgcagg agaatggatt aacggcgatg gctatcaaca caatgtgttt
     1081 gtgaaatggc acaccggcga gattgtccgc cctaaaacgt ggttttggca agatgttcgc
     1141 acccgcaaga ttttaggtta tcgaacatcg atttcggaga atactgacag catacgccac
     1201 gcattaatgg atgtgatttt taacgtaggc ataccgaaaa cgctcacgct ggataacacc
     1261 cgagcagcag cgaataaggc aatgaccggt ggtattgaaa accgctaccg ctttaaacat
     1321 accgagttag acccgaaagg cattatgccg attttaggga ttgatgtgca ttttacctca
     1381 attctttatg gtgaaggaca cgggcaagcc aagccgattg aacgtgcttt cgggcgaggc
     1441 ggaattggcg agaaaataga taaacgccct gaattaagtg gtttctatac gggcagaaat
     1501 gcacaagaaa cccctgataa ctataacggc ggtaaagacg gtgtggatta caacacattc
     1561 ttgaaagcca tagcagcagg gatagaagag tacaactctc aaaccgaacg ccataccgaa
     1621 atgtgccgag gagaattgag ttttaaccaa gtttgggagc gggattacca cccaagcaac
     1681 gtaagacaag ccagccctga acagttgcgg ttactgttct tacaggcaga aacggtcagc
     1741 attaaacgca acggcagttt tacgctgaaa gcagccggca aactttacgg cttaactaac
     1801 gtttactggg cagaaagcct gatagggata acggataaga aagtagtggc acggttcgac
     1861 cccgatgact tacacggcaa cgtgtatgtg tacaacctag aggggcaatt cttagccgaa
     1921 gcggtttgcc gagaggcgaa aggatttggt gataccagtg caagccgtga gcaaggtcgc
     1981 ttatatcaga aagtagtgaa aagtgccaaa gctcaagccg aagcccttga attactggaa
     2041 gctcacgaac tggcaagcct cgccccacaa gtggacgtgc ctgagccgat agagaagaag
     2101 gtaaaagagg tgctggtgaa agaagatttt atcgtggatt tcaacactgt gcgtaaagca
     2161 acggtggtgg aagaaaccga agagatcagt atttttgatg aagtgttact gaaacaagag
     2221 aaacaacttc gcatagtgga ataactagcg gtcagttttt gcaaaaaatt tgcaaatttt
     2281 tctaaaaaaa cgaccgcttg aaatgagatt taaacagggt ttaaacgcct ttaaggagta
     2341 aaaaatgaca ttaacagggc aagcttaccg agtgattttc aaggaactca aagagcaaca
     2401 agagagtgaa tttgaacagg ctttcaagaa agggattgcc aaaattcagc agaatcaaac
     2461 tcaggaatcc attctggtaa accgtgatat gtttcaagct gaaaagctaa cactgctttc
     2521 acaaaagttg gaagcggttg agcaattact tcgccttgaa ttaaatcagc cgaattttca
     2581 agcaaaactt gaagactacg ctgacgcatt acgaacaacc attttaagta aagtagccaa
     2641 ataagagctt tagactcttg aaggcttaac atctgagcca ctttttttac tgtcggcatt
     2701 ataccgtcaa gatcatcaat agaagttaaa tgatgaaaag aggaatggta ttcagctaaa
     2761 caaaaattga ttgtactaag tttaaacaaa gtaatgcaat ttttcatcac gatggcattt
     2821 gctcgtttta acttaaaacc agagaaggtt gcacgagcag aatcaagggc tatttgatga
     2881 atttcatcat ctgttaaaga acgaaagagc ataaaaacct ccaaacaaaa acaggacacc
     2941 attatgacat taatcgacca aattaaacaa cacatcgccg aaagcggcag ttctcagggg
     3001 aaagttgcca aagaagccgg gattaatgcc ggggcgttat cggcttacct gaatgaaaat
     3061 tacaaaggca ataatgaaga acttgaagca aagctgattg cttatttaga gcgtattgag
     3121 gcgaagaaaa gagagtttgt cgaagctccg agttttattg aaaccaaaac cgcaaaccaa
     3181 attttcggtt cgttacgatt tgctcaaaat actggcgtgc ttgccattat tcacggtgcg
     3241 agtggcgtgg gtaaaacaca agcggctaga gagtatcgta aacgctatgc gaatgtatgg
     3301 ttggttaccg caagcccttc tcgttcctca ttaagtgagg tgttatatga aattgctctt
     3361 gaacttggaa tgaacgatgc accacgccgc aaagggacat tggctcgttt aattgttcgc
     3421 aaaatgaaag gcacagcagg tttattaatt attgatgaag cagaccattt accttacgaa
     3481 gccttagaag aattaaggct gttacaagaa gaagtagtga atgaaagtga catcggagct
     3541 ggtgttggct tagtgctgat tggcaatgac aaagtctaca cccgaatgaa aggcggaatt
     3601 aaccctgctc acgaatatgg gcgactttgg tcacgcagtg ccaaacgcac cagtattcag
     3661 aaaaccaaaa aagaagatac ccaagcggta gccaaagcgt gggggttgag tgaaaaccaa
     3721 gaagcactca aggtaatgca agccattact gaaactggtg gcggattacg gattctgaca
     3781 cacaccttac gattagcagc gatggtagca aaagaccgaa tgattccctt aactgccgat
     3841 ttaattcagc tcgcacgtaa agatttactc tcaacaggag attaacaatg agaaccccaa
     3901 aacccaaaca cagttttaac cgcaccaacc aagcagcgtg ccgtttttta aaacaaaccc
     3961 aaaaggcaat tatccgctta aacagtcttg gctttacggt gctgaatatt gattttaccc
     4021 gtattaagcc ccgcattgag gtggaaatcg gcaataacaa gcacattgct caaggcttga
     4081 tttatgaggg taaagcctac cgctacagct tcggtaaaag cgaagattta ggcagatggg
     4141 aaggctacta caccatgctg gaaggtatca gagtgtggtg gcgacaagca gcaattcact
     4201 aggagaaaaa cagatgaaaa ccttagcact attaaccgcc cttttagggc ttgtagggtg
     4261 tgatttagtc caaagcaaac aagctaaaaa ttacatcggc aacaatttaa ctgaaatctg
     4321 tatagataac gtggtgtacc tgatttattc cggtgataaa aaaggcggaa ttaccccgaa
     4381 agtaaataaa gatttttacc cttatacctg tacaaacaaa gaggaaagac aaaatggcaa
     4441 aacccgatga aacacccatt acaaaagcag aatgtcaatc tcagcttgca gagttggggg
     4501 tgcaatataa aaaactgcca atggcaatta caagacatat ttgtaatgcc acaaccaata
     4561 tacacggcaa agttataaag gtcagtgtgg tagagagagt tggatatggc gtccagatta
     4621 ccgcacaagg taacgagaaa tcttgcttag taacctatga agcaatgtta ggtatggcag
     4681 aagcaatggg attatttgat gaagttaagg agcaaaataa tgactaaaaa aacaaccaga
     4741 gtaaaaagtg caacccaaag tgcgatttat caaagccgag atgaagtgca agtggctata
     4801 aaaatcattg gtgataaaca gcgtgaattg caacgtttag ctacggcaat gaatgatgaa
     4861 ttagctgcta ttagtgccag ttatgctcaa cataaccgct accaacatta tgccgagaaa
     4921 gcggctgaag cagaacgcaa aggcaactat aaggaagctc aagaccattg ggaagttgca
     4981 aaattatcag caaaaaagac cgctaaccga gactgggcgg aacaacgagc ggaattttgt
     5041 aaacgtatgc acaataaacc gttttagggg gaagtgatga cagaaaccgt aaaagcacaa
     5101 cttaacagcc aattaaatga ggcaattatc cagttgatac aagctcaaaa gtatttaaac
     5161 caagatgatg ccattcgcag tggagtgtat atcggtacgg tgcaggattt attgccaaag
     5221 gtgcatttaa agttattaac ggcaaatcgt aaacattgat agagatgagg taaaaatggc
     5281 tattacagaa gaacaatgga aagaaattaa gcaaaagcta gatagtatca tagggagagt
     5341 taaatttaga tacaaagagc atttactcac ggtagatgtt gtacaaatta agcgatcttt
     5401 aaaattggct gtgtatgttg atggggaaat tgatggagct tggacaaaag aaggacacga
     5461 aattcgccct tatttagaag aagtttggta tcgcaaagaa cgcccttttt ttaatgctaa
     5521 ggagaagaaa gagtatagag gtttaatgag caaaaaaaaa ctgaatgaaa aaatagtaac
     5581 ctactcacct atgtttccat caccaactgc actgactcgt caatataaga aactagatgg
     5641 cttagagttg attgaagtga tttaaaaccc atttacagcc catttaaccc cgagttattt
     5701 aaaggacaac aaaatgaata aaaacgaatt ttttaagaaa taccacctag agggttcact
     5761 agctattttg gatttggatg caaatgacga tcaaagccaa ttctgttacc aagcattgtt
     5821 ggagtataga gaactcccgt tagacacatt tcttgcagag tactcagatg atgaggatat
     5881 gacagagtca gagtttattg agttaatgct tgatgaatta gagtggatta tccgcagagc
     5941 taagaatgct aaacggtttg ttaaaaaacg acaaaaaacg atgagggagg aaattatgta
     6001 acagctcaac ccaaccaacg ccacatcatc cgtctagctg agaaacaggt gtggcgtttg
     6061 ttttaagtgg gcaatcaaaa gcgataacgt ttttaattgt ttatttatcg gagtaacaaa
     6121 tgacagacaa agccaagcta atccagctta tccatattgc caagtcgcaa ctagcaatgg
     6181 acgatttaag ctaccgtgaa atgctgaaac ggctcaccaa taaaaccagc tcgaccaaat
     6241 gcacggtggt cgagttgcac aaagtcttac acgaactgca aaccaaagga gcaaaagtaa
     6301 aatattttgc caaacgcagc aaaaaaccga ccgcttacag cccggcaaca ggggaaacag
     6361 cggtaaaaag ccaaattgct cataaaatcc gagcggtatg gataacaatg ggcaaacacg
     6421 gatttttaaa cgacccaagc gaaaaagcat taaatgctta tatgcgtaag gttatcaata
     6481 ataaaactag accaacaata ttattaaatg tctctgcatt agaggctgga gatgcaagtt
     6541 atatgcttga aatcctcaaa aaatggcata aacgtgtgat gattaaagcc ctaagcaaaa
     6601 gttgcacagt tgatccaaag ataagctacg atgaattagt ggagtggtac aatgaagtta
     6661 tgtagatgcc cgatttgcca cagtgatata cacctagaag ctctcgtaga agatgaagcc
     6721 gggcgtgaac tactcggcaa aatcagccaa ttaacacacg gtgttgctca accgatggtt
     6781 gcttatttag gcttatttaa accgcaaaaa agtaacctta ataactcaag agccttgaaa
     6841 atactcaatg atgtattagc actctaccct tgctcattgc tactggctca agccctatct
     6901 gaaacggtag taagcatacg caaaaaacgt cagcaagcaa tggaaaacgg gcagaaaatt
     6961 gaaccgctac cgaaccataa ctatttaaaa tcggtgtatg aaacgcaaaa agtccatttt
     7021 gctgtggtga gaaccggcaa aaccgaacgc gaaacaatga aagccgaact ggaaaaaaca
     7081 cgcaatgcga ttgcctatgt gcaacggttt gttgatttag gacgtgaaga ggttgttaaa
     7141 aatagccctg agtatcagat ttggttagct cataaaccca aataacacaa gccaccaaaa
     7201 ggtggctttt taactttttg aaagtgcgat aaaacctgat ttaaacaggc tttaaacggg
     7261 ttttaagatt gagtttaaaa aaatatttca caaaataaga atggtttttt atacaataac
     7321 cgaaacggtt tttggatagg aaatagacaa tggaattagt ggatattttt gaagaaaaag
     7381 ccccggaagt gttaaccgat ttagccaaac atattgaagt cgcattagaa gaaaaatgcc
     7441 aatttagccc cgaaaaggca aagcaaatcg gtattgatat tgcccaacaa atggctcaaa
     7501 actggggtgg cgaggtaatt tatattcctc gcaatttgtt gatggctctt aatgagcgag
     7561 atatgaaaat ttacgaagag tttaacggta acaatcaccg tgaattggct cgtaagtaca
     7621 atgtatcaat gcagtgggta tataaaattg tgaagaaagt ccataaggac gaagttgccc
     7681 gcaggcagat gagtatgttt gagatgtaaa tcattttttc ccagccctga tcattttcag
     7741 ggctttttgt gtattaaaag aacgcagtct ttaaacccgt ttaaaatcaa tttttcagca
     7801 aattctttaa actcctttta aagcaatttt aataggagtg tgctatgtct caaaatctta
     7861 cctttcgcca aatttttgac cgtttaatcg gacacgaggg cggctatgtc aatcacccac
     7921 aagacccggg cggagaaacc cactggggaa ttaccaaacg taccgcggtt gctaatggtt
     7981 atatgtatgc aatgaagaat atgacccgtg agcaagccta ccaaatctac gaaaaagcct
     8041 tctggcaacg ttatcgttgt gcagagctta agccagcagt tgcttatcaa tttttcgacg
     8101 cagtggttaa tcacggtttt ggtaatgcta gtcgtatgtt acagcgtgca gtaggcgtag
     8161 cggatgatgg cattattggc aatattaccc tgcaggcaat taatgcaaaa tctgaaagcg
     8221 atctcttaat gctatttaat gccgaacgtc tgaagtttta cacaaaatta cgcaacttcc
     8281 ccacttttgg taagggttgg gtcaatcgtg tggcaggcaa tttagaatac gcagcacaag
     8341 ataatgaggt ttaaaaatgg caaaactatt taaagaacta ctctcaacca atggcacatt
     8401 atccaccact agcaccgtgc agttttttgg cacattgtgt gtatttggtt taatggttta
     8461 tgcagtaatt acccaacaac cttatgccga aagcctatta aataatgtgt tgatttatct
     8521 ttttggggca actaccgcta agggtgtagt aaccagctat caagccaaaa ttaaaggggg
     8581 tatccataaa caacaaagta catttaaaga ggaggaggga tgttaatgca acaaatttta
     8641 ggcgtgattg gcgtcatcgc agcaatattg tcttatgctt tgtttaaaag ctggcaacta
     8701 aaacgtgagc gtaaaaagaa tgccatatta caagctgaaa aacagcaaca agcggttgaa
     8761 attgagcaaa aaaaagcaga ggtaaaatat gtgtatatat caaagaaaaa caatgaaaaa
     8821 gttactcgca gctctgctag cgatattgat gacaagttgc agcaacacgg ttacttccgt
     8881 gacaacaaca ggttgcacgg cgttcagtct gatttatccg aaccgtgcgg atacgctgga
     8941 aacgaaacgt cagattcttg ctcataatct gacttttgag gaaatttgcc aaaatgaaat
     9001 acattaaaaa attacgtaaa cgctggcaaa tttggcgttt tttaaaaaat catcctgagg
     9061 ttgcacaacg ccgagattta ttagcgattg ccattaaaca gggcgtagaa aaccctgtag
     9121 ggcgtgcaag acggagggag ccataatgga tttatttaca gtcttttggg agtctatccg
     9181 tgctaacttt ggttttattt ctgcgttact caccattgtt gttacggcat tttggctcaa
     9241 gctagatagc aaatacgcca agaaaaatga tcttggtgaa ttgctggaaa ttgccaaaag
     9301 ccacgataac cgcttaacca ccttagaaac caaggtagaa aacctgccaa gtgcggtgga
     9361 cgtggaaaag ctgaaaacct tagtcactga tgtaaaaggc gataccaaag ccacctcaaa
     9421 gcaggtggat agtatcagcc accaactcgg cttgttatta gaagcaaaat taaaggaatg
     9481 atatgtcgtt aagagattta ctcactcaag accaacggct ggtgatttta cgctcattag
     9541 cggaagccgg ctatgatgcc aatgagtcaa ttattagtga ttgcctagat ttatacggtc
     9601 acgatattag ccgtgattta gttcgcaccc atttaagttg gcttgaagag caaggcttac
     9661 ttaccttaga acgtttgcga gatggctata tggtggcaag cattacccag cgtggcttag
     9721 atgttgctca agggcgtgtt gtggtaagtg gcgttaaacg ccctcgccct aaaatttaaa
     9781 cggtatttaa ggagcgttta aatgagcgag aaaaatactc gtggtcgagc atcaaaagtc
     9841 gatttactgc cgccgaatat taaaacccaa ctggcgatga tgttgcgtga taagcagtac
     9901 tctcagtcac aaattttaga agaaattaat gacttaattc gggattgtgg cttgcctgat
     9961 gaaatgctgt taagcaaaac cggattaaac cgctacgcaa gccgtatgga acgcttaggg
    10021 gcaaaaatcc gtaatgcccg ggaagttgcc gaaatttgga ctaagcaatt cggcgaaatg
    10081 ccacaaaccg atattggcaa aaccctaatg gaaatggtca agcaaattgc ctttgaaacc
    10141 tcattgaaat taggcgaaca agaaggcggt attgagccaa aacagcttgc tttgctctcg
    10201 tctgctattc aacgtttaga acaagccgaa agtttgagtt ttaaacggga acaggcaatc
    10261 cgtaaagagg tcgcacaaca agccgctgaa accgctgaaa aagtggtggt gcaagcgggt
    10321 ttatccgctg aaaccgtccg tacgattaaa gagcaaattt taggaattgc ataatggcac
    10381 tactcaataa cagaccgctg aatgaattag cccctgaatg ccgttcgttt ttagagagca
    10441 ttcacgcttt taatcctaac gaactgttat tgggctatca aaagcggtgg attgcggacg
    10501 acagccaact caaaattgcc gaaaaaaccc gccgttgcgg tttaacgtgg gcagaagcag
    10561 cagataacgc cttaatcgca agcacccgca aatcagacgg tggctctgat gtattttaca
    10621 ttggctcaaa taaagagatg gcacgtgaat atatcgatgc tgtcgcaatg tgggcaaaag
    10681 cctttaatcg tgtggctgga gaaatccaag aagagatctt tgaagacgaa gataaagaca
    10741 tcttaaccta tgtaatctac ttctcatcgg gttttaaggt caaggcatta tccagtaatc
    10801 cgaaaaacct gcgtggtatg cagggcgtgg tggtgattga tgaggcggca ttccacgaat
    10861 acttagcgga agtcttgaag gcggcgttag ctctcacaat gtggggggcg aaagtccgta
    10921 ttatttcgac ccacaacggc tcggaaaatc tgtttaatga actgattacc gatagccgtg
    10981 ccggccgcaa acgctactct gtgcatacga ttacgattga agatgcctgc cgtgatgggc
    11041 tatatcaacg tatctgccaa gtagtgacga aaaaaacgtg gtcaccggaa gcagaaaaag
    11101 agtggattga aaacctactc aacgacaccg ccagcgaaga agacgcattg gaagaatact
    11161 tctgcgtacc gaagttcggc tcaggcttat ggctctctcg tgctttgatt gaacggcaga
    11221 tgagcgaagc cacgcctgtt atccgcttta cggcaaaaga tggtttcagc cttgtggctg
    11281 aaccgacacg ctatgcggag attgccgaat ggtgtgaatt agaacttgag ccgatattgg
    11341 caacccttaa ccctgatttg ttgcatttta tgggcgaaga ctttgcacgc agcggcgata
    11401 tgacctctct ggtggtgctt gctcagcaac aaaaccttgt tcgtaaagtc gcctttattg
    11461 ttgagcttgg caatatgccc tacaagcaac aagagcaaat cgtgatgttt atgcttaaac
    11521 gcttaccgca ttttgccggt ggggcttttg acgcccgagg caatggtggc tatttagccg
    11581 aagctgcacg agatgaattt agcaccttga ttgactgcgt gcagttatcg gaaaaatggt
    11641 atcgggaaca caccgccccc tttaaagcgg cattggaaga cggcgagctg gaagctattc
    11701 caaaagatgc ggatattttg gctgatttgc gttcatttca ggtggtgaaa ggcgtaccac
    11761 gcataccgga taaacgcact aaaagtgcag atgggaaaag caaacgacac ggtgataccg
    11821 caatttcttt gctgttggct cattatgcca gccgacaatt agtgcaaatg ccagttgagc
    11881 cggtatcacg ccgtccacgc catagccaac aattaacaga aggatattaa caaatgagat
    11941 atgtaccact ttgtttaatg atgtgtgcct cattctacct atttattaac aacattgacg
    12001 gctggggttg gggaattctt ttatctttta ttttagcgag tgcagcttat ggcaaagaag
    12061 aataatttaa ttagtgaaat tgccacccgt gccagaagta ttgattactg ggcatttggc
    12121 tattatctgc cgaaccctga cccgatttta aaacgaatgg ggaaagatat ttccgtttac
    12181 cgtgagttgt tatcagacgg gcaagttcgc tcaggcgtac gccgtcgaaa agcggcgatt
    12241 aaaggcttag agtggcgaat taccaccact aacaatgaga aagtggacga gcagatttat
    12301 caaatattta accgcttgcc gatgaataac ctgattaccg aaatgctcaa tgcagcactc
    12361 tatggctacc atatcagcga ggttatttgg gcaaaagagg gtgagttatt tgtgccaaca
    12421 gaaatcattg gtaaaaagcc tgaatggttt atgtttgacg acgaaaacct actgcgtttc
    12481 cgtaccaaag aaaactgggc ggacggcgaa ttattaccag atgataagtt cttgcttact
    12541 acgcaagaag ccacccaaga taatccctac ggtttaggcg atttatccct ctgcttttgg
    12601 gcggcgacct ttaaaaaagg cggtttcaaa tactggttgg aatttaccga aaaatacggc
    12661 tcaccgtggt tgattggcaa acaccctcgc caagctcaca atacagataa agagcgacta
    12721 gctgatgccc tagagggaat gattagctca gcgattgcgg ttatccctga agatagttcg
    12781 gtcgaaatca aagatgcagc gggcaaatct gccagcaccg atgcctttga aaagttcctc
    12841 aatttttgca aggcagagat caatatcgcc ctacttgggc aaaaccaaac caccgagcaa
    12901 gaaagcaacc gtgccagtgc acaagtaggc ttggaagtta tggaagatat tcgctcggac
    12961 gaccaagcga tgattgaagc caccttcaac accttgttgc agtggattgt gaaatataac
    13021 ttcaatgtgg aacaactgcc gaagtttgaa ttttacgagc aagaagccat caacaccgac
    13081 caagttgaac gagacagtaa actccacgca atgggcgtgc gtttttccaa agcctacttt
    13141 gagcgtgaat atggctttga ggcgggtgat attgaggtgc aagcggtcga aaaagcggaa
    13201 aaatctgcaa ataccacaga atttagcgaa ggacaacgcc gaacccaagt gccaatgcct
    13261 gtgccaattt tggaacagct agaacacgag ggagaagcaa tggtggacga ttggctacaa
    13321 ggggtgagag ataaactcgc tcaagcagat agtctggagg atttccaaag tcagctagat
    13381 agccttatcc ctgaattaag ctttgccgaa tatggcgagc tgttggcgtt aggctcaacg
    13441 gtggcagagc ttgcaggcag acaatcggta aacgatgaaa ttaaggcaaa acgcaatgcg
    13501 taaatttacc tttaaacagc aagcccaata ttttgagaaa aaactcaatc tccgcacgaa
    13561 tagctacctt gatattctgg gcgaagagca cgactatttt ttcgtggtgg ctggggcgaa
    13621 tcgtaatgaa gtgttgagtg agtttcgcaa ggcggtggat gaggctattc acaacggcga
    13681 aaccttagag aactttcgca agcggtttga tgaggttgtg gcaaaaacag gctgggagta
    13741 caacggcgga cgaaattggc gaacacggat tatttacgat accaatgttt acggggcgta
    13801 taaccgtgga cgtttgcaac aacatttaga gcttgcggag gatatgcctt attgggaata
    13861 tcaacacaac gacaacgccc accctcgccc acagcacgtg gcgtgggacg gcttggtgct
    13921 gcgttatgac gatccgtttt ggaaatatca ctatccaata aaagcctacg gttgccactg
    13981 caccgtgcaa gcgttagatg attttgacct caagcacgat ggtaaaaccg tcagcccacc
    14041 acctgaaatt gagtttgaac aaaaaacggt aggcgtgcgg acaggcaatt cacgagtcgt
    14101 aactgtgccg aaaggggttg atgtgggttt tacaccgcat aattttgata atctcaaagc
    14161 cgggagagca gcaaatgtag ataagttgct aatgcaaaaa atggttaccg ccgatcctat
    14221 atttgcctcc aaagcagtca gtgatttatt ggcaaaagag ccggttgcaa agttacataa
    14281 ccaagccttt aaaaattggg taaatgatgt tgccaatgct aaaggaagtg cattatccca
    14341 agtagagaat atgcagttgt taggggtact acctgttgcg gtgattgaac ggttacagca
    14401 gatcaattta gctccgcaat ctgcaattat tgcaataaca aaaggcgata tggtacacgc
    14461 tctacgagag ctaaaacaaa ataccggtat tgccgtaccg ttggaatttt gggaaaagtt
    14521 gcctgcaaag ttacaatacc caaatgccat cttgttggaa aaacaacaag cagaaactac
    14581 attgctcttt atctatgacg tagctcaaac caaaattgcc gtaaaagtca attatgatgc
    14641 aactttacaa gatagacacg gagcaaaagg acggcagaaa gtcttactga atcgagcggt
    14701 tacaggtagt ttattccaag atacaactgg attgaaaagt tcaaagtatg aagtgctatt
    14761 gggagaaatg tagcggcgaa ggactcgaac cttataatgt aggttaaacc tacaaccttt
    14821 accaattagg aaactaccgc tacattgtgt aaattcacta tacccctaaa ttatttttta
    14881 atcaatagga gaaacaaaat ggcagaaaaa caactttcct ttaaagtagc cgaaatagtg
    14941 ccaatgtcag ctattaacac tatgcggcac ttaattagtt tggcattaca agcgaattat
    15001 gagagagaaa aatctcgtgc ttgataattt caatggcttg tagttcggga actatattat
    15061 ctgaaacaag ccattgtttt gttgatgtca gacatttagc gatgaaatcc ataattaaac
    15121 cattagcagt agctcgttgt tccggagtaa gttgtaacca cgacgcctct gtttgttggt
    15181 ctaatgtata aagaaacata acggcttctt tttctgataa tgggtcaagt ggtaattttt
    15241 cagcttttgt gactaaccat tgacgggaaa gcaataaatc ataaaccagt tctattgctt
    15301 gttctgcatt taccatcata ttctaattcc ttaatatatc agggcaacat tacccaatct
    15361 atattttact gagatttgct taatgatcaa aatcaccctc aacaacaccc aagcggtcaa
    15421 atcgttacaa caaattgcaa atcagctgga acaacctcgc cgactgtatg gcattttagg
    15481 ggaaacccta aagaaaattc acactgcacg ctttaaagcg gaacaagacc ccgatggcaa
    15541 caattggaaa ccgttagcca gtagcacgct tgctttaaaa cgtaaacgag gtaagtccac
    15601 taaaatctta cggcaagacg gctaccttgc cgataaaacg gcttacaatg ttacaagtga
    15661 taatgttgag tttggctcgc aggaagtgta tgcacggttg catcaattcg gcggtaaagc
    15721 cggacgaggc aaaaaagtga ctgtaccggc tcgcccgtgg ttaggcgtag gcgataaaga
    15781 tgagcagtta cttttacgca aagctgagtg gcatttaggg caaattatcg gtcgtatggg
    15841 aaaataaccg cttaaaatca aatttaacgc ataaaaacgc ctctgtgcgt ttttattttt
    15901 tattcgcctg attattcgac tcaaaaaatt taaacgctgt taaacgcatt taaacggtat
    15961 ttaaacggta ttgccgattg tgataaatcc tctttattaa aacaccctcg aaaaatcttt
    16021 aaaccagttt aaaagctaat ttctctccaa atccttatta ttgccccatc acggaggcaa
    16081 tatgacccaa attgaaattt ttaaagccgg taagcgttat gacgccaacg gcacgttaat
    16141 tgacattacc cctgaaatgt tgcaacagac cgttgctgcc tataaccccg aatttcacga
    16201 agctccgctg gtgatcggac acccgaaatc caacaaccct gcgtgggggt gggtaaaagc
    16261 cctttccctt gagggcgatg tactaaaagc agaagtagat cagctagacg ctgagttttc
    16321 agaaatggtg gcaagcggca aattcaaaaa agtctcggct gctttttact tacctgatag
    16381 cccgaataac ccacataaag gcgtgttatc gctacgccac gtggggtttt taggggcaat
    16441 gccaccggcg gttaaaggct taaagcaggt ggaatttgca gaagatgatg attttgtcgt
    16501 cttttctgaa tggggggttg caagtctatt tagtcgtttt cgtgattggt tggtgagtga
    16561 gttcgggctt gaaaaagccg ataaagctct tcccccacac gaaattgatt ggctgaaaga
    16621 agaggcaatg cgaagccaaa ttagcaaaca ggtacacgcc gaacaggcaa cacctgaacc
    16681 gatttttaat gaacccgcac aaccagaagg agaacctgaa atgagtgcag aagagaagga
    16741 agagcttgaa cgcttaaaag cggaaaacga gcagttgaaa gccgaaaaag cccaagccga
    16801 agcagagaaa atggaagcag agctggaagc tgaaaaagct ggtaatgcag agttttgtga
    16861 aggtttagtc aagcaaggca aacttgcccc ggtggcaaaa gcggcgtttg tgcaggcttt
    16921 aaataacctt tcagtattaa aagccggtcg tgagccggaa tttaatgagg gcgaagatgt
    16981 ggtttcacag tttaagtctg caatgtcgca atcaccacag attatccaat ttggagaata
    17041 tgccacagca gataaagcca acccacaaga agcggaagca gtggaatatg ctgaaggcac
    17101 agatccagtt tcgattgaag ccgaccaaaa agtgcgagct tatatgaaag cccacaatgt
    17161 aggctacgct gcggcattta atgcgattta tcgctaatgt ttaacccaca actgaaaagg
    17221 aatactaatg acaactcata acttacaaaa actgcgtgta caagaccccg ttttaaccga
    17281 gctggcacaa ggctatcaca ataacgagct ggttgccgag accttaatgc cgactgtcga
    17341 aatcgaaaag gaagcaggca aaatcccaac tttcgggcgt ttagctttcc gattgccttc
    17401 taccgtgcgt aacttgcgtg gttcatctaa ccgtttagat ccagaagata ttggggcgat
    17461 tgatgtggcg ttagaagaac acgatgtgga atatgccatt gattatcgtg aagagaacga
    17521 agccattttt tctttacgtc aatttgcctt aaataccacc caagatgtga ttgcgttagg
    17581 tcgtgaaaaa gcggttgcaa ccctcgcctt agatgaaagc cgttatgaaa cagacaataa
    17641 agtcacgctg tccggcacca gtcaattcag ccataaagat gctgatgtgt ttaaggtatt
    17701 tgataccgct attcgtgccg tgaagcgggc tatcggtcgt aaaccgaatg tgtgtgtgat
    17761 ttccggtgat gtgtgggcag cgttaaaaga acatccggca gtggttgaaa agctcaaata
    17821 cagtcaagta gcgattgtta cccctgaggt gtttgccaaa ctgatcggca ttgagaccgt
    17881 aaaaatcggt gaagcggtgt atgaagaaag cggtcagctc aaagatattt ggagcaaaga
    17941 tattgtggtg gcgtatgttg ctcctcgttc gaccggaggc aaaggcacgg tttacgagcc
    18001 gtcttatggt tacacggttc gccgtcaaaa aggcttattt gttgatactt ataaagagag
    18061 tggcggcaaa attgaagtgg tacgcacgac cgatattcac aaacctcatt tagtgggtgc
    18121 ggcagcaggc tatttaatta aagctgccgt ataagccaca taccataggg tgcgtttact
    18181 aacgcaccat tatctcaaac acattacata gaggaaaaaa cgatggctaa atttattgtt
    18241 ttacataccc caattttaca cgatggcaaa cgctatgagg tgggggcaga aattgaactc
    18301 accgctgaac aagctgagaa caatgcgatc aacttaaaag cggtagaagc tgttccaaaa
    18361 ccacctaaaa atgcctcaaa acctgaagat gagccaaaac ccgaaggtga acctaagcct
    18421 gaagatgcag tgacggaaga tgatgtgaag ctggctgaag caaaaattgc agaagcagag
    18481 caaccaaaag ggaataaaaa ataatgtatg tgcagattga tgagctactc acggcgttta
    18541 gtcgcaagat tgtggtgcag ctcagtaatg atgaaccgac agcgaccgag cctgatttaa
    18601 agatgcttga aacggcagtt aaagtggcaa atgagcgaat tgatgcagcg ttacgctctc
    18661 gctatacctt gccgctggct gatgtgccta cgcttattaa tcagcacgca ttaacccttg
    18721 cccgttattg gctttatgcc cgccgtcctg aaaccaaaat gccggacacc gtgaaggaaa
    18781 cctaccaaca agcggtgaaa gagttggaac agattgcaac aggcaaactg catttaggca
    18841 ttgctgaaag cagtcaaatt ttagaagacg atgttttagc cgataacagt gaatatgaag
    18901 ttcgggcgaa cgctcgcatt gataccagtc gttattaaag attatgtcag ccacacaacc
    18961 gattttagaa gatatttgcc aacacttgcg gcaagcattg cccgagtggg aggtggaact
    19021 cttcccgaat gacccaagca cctatcatct ctcgcacatt aacggtgcgg tgttgattag
    19081 ctatttggca agcaaatttg agaaacctcg cactacacaa gcggtgctgc aggttcgcca
    19141 cgttcagatt gctttaacgg tgctgacccg tgatttgcat aatgatgtcg gggcgttgga
    19201 tttgctggat aaattgcggt tattggttgt ggggtttaag cctcaaaact gcggtgagtg
    19261 ttggttgatt gatgaatttt ttaatggctc aagcgaagaa accgggcttt ggcaatatca
    19321 gttgattttg caaaccgaaa ctcagcaggt gcaacaccaa gcggtcgaaa atctgccaaa
    19381 atttgtaacc gctcatcttc gccgtcaaga ccagcctggt tcgcccggac ttaaaactta
    19441 aatcgtaggg tgcgtttgct aacgcaccat caacatcacc tgattttacg gtgcgttggc
    19501 aaacgcaccc tacaaggaga atattatgac gtttcaccac ggttctgaaa ccgaacgagt
    19561 aaacggtggc tctgtgccgg tatcggtggt tgatagtgcc attatcggca ttgtcggtac
    19621 tgccccaatc ggcgcagtta atgaattgac cgtctgttta accaaaaaag attttgccaa
    19681 attcggcacg attttaaacc aaggcttcac cttacctgat gcctttgata ttttagctcg
    19741 ttacgcagcg ggtaaagtgt atgtggtgaa tgtgcttgac ccttcccgcc accgtactac
    19801 cgtgaccgat gaagtcctca cactggatcg caatacgctg accgctcaaa cagcgaaaac
    19861 cggcattatt gcggtgacga gagtgaaaga cggtgcaaac gtgctgaatg ctgatcggta
    19921 ttcggtcgat ctgcaaaccg gcgtggtgac ctttgtctct gcaccgtctg ccccgaaaat
    19981 tagctatatc tatgctgacc cgagcaaagt gaccgaagag gacattaaag gcggggtgga
    20041 tagcttaacc ggtaaacgca aaggttttga actattgcgt gatggtttta acctttacgg
    20101 tgcggatgcc aaaatcttaa tctgtccgga atttgataaa accgcaagct gtgccgctgc
    20161 ccttgccacc cttgccgacc aactgcacgc taaggcgtat attcaactgc cgaaagggac
    20221 gagcttatca aaagctattc aaggacgtgg cccggcaggg acgattaacg cctcggcaag
    20281 caatgagaac gtacgccatt tcttcccgta tgcgttgggg tcaagcaata ctcttgaaag
    20341 ccttgcgacc cacgccgccg gtttgcgtat gttgattgat gtggagaagg gctactggtt
    20401 cagcacttcc aataaagagc tgaaaggggt gatcgggttg gaaattccgc tgacggctcg
    20461 ggtggacgat aagcaaagcg aaactaatct gctcaatgcg gtagggatta ccaccatttt
    20521 caatagtttt ggcacaggct tccgcttatg gggcaaccgt tcgtctaact tcccgactgt
    20581 gacccatatc agcaactttg aagtcgcctc tcgcacaggc gatattattg atgaaagcat
    20641 tcgccaagcc gaattgcagt ttattgattt accgattgat gatgcgttgc tggatagctt
    20701 ccttgaaacc atcgacactt tcctgcgtac gcagaaatcg ttggtaggtt attcggtgag
    20761 cttagactat gaatatgact tggttgatgc attcagccgt gggcaagtcc cgattgtgta
    20821 tgaatacact cctaaaattc cgggtgaacg tatcagcaat aaatctgtga tgacccgtaa
    20881 atacttggca aacttggtgt cgcaacgata aggggaaata aatgagtatt gcaattaacc
    20941 aaattgtaaa cggaaatgtt tacattaacg gcaacagcca aatggggcga gtgaatgagg
    21001 tcaaaatccc cgatattgag tttgaattta tcgaacacaa aggccttgga atgcacggtg
    21061 tgattaaatt acctgccggc acgaatgcga ttgaagcaga aatcacttgg gacagttttt
    21121 atccggaagt gcgtgcggta atgcttaatc cgtataaata cactcagttg caaatccgct
    21181 ctaacctgca agtgtttaac tcgcagggct tggcagccga agagccgatg gtcaccatta
    21241 tgaatgtgtc tgccggtaaa atcggcggca cagggcataa aaacaaagaa aatgccgaat
    21301 ttgccgacac gttaaatgta cattcaatta aacagaccgt tgccggtcgt gagctgttat
    21361 ttattgatgt gctggcgaat atctaccgtg tgaacggggt ggatgtgttg caaaaattcc
    21421 gcaccaatat cggttagtaa aatctttaaa gccgtttaaa atcaatttaa acaccgttta
    21481 agtaaactcc tttgtgaagt taaacaacaa ccactcacaa aggagttttt ttatggctaa
    21541 cgcagctcaa caagtattaa ccaatcttcg tgtacaaacc acttataaat tgcaataccc
    21601 tatcgcttta ccagatggta gagttattga aaccttagat gtacgtcgtc caaaaggtaa
    21661 agattttcgt ttgcttgatg aaaaagggtt tgaccctgaa aaagatggcg taaaaattct
    21721 acgcttctat attcaacagc tcaccacact tgtgccggaa gatattgatg aactcgatgc
    21781 tgccgatatt aagggcttaa ataacctatt ggaagatatg cttattgagg gaaagtccga
    21841 caaataagtg aactagatac cgtttatgct gatctcgctt ggtggtttaa gttttcacca
    21901 agcgagcttt tagaattgga tttagtggat attccgaaat gggtagaaca gatgaacagg
    21961 caaataaaag ccggttatgg gcagatttta cggtagagcc aaataaaggg ttcggtggct
    22021 aaagtaagta aaatggcgac gccccaagct agatacacac caacccacgc caagattgtc
    22081 aaaatcaatc cataaatgag tacaaaacta acgccctcgc ttaggctttg tatagattga
    22141 atcgcatcta accagtagaa gatactccaa ccgactaatg ctaccgctaa caaccattta
    22201 gcggtgcatt ctgttcttgt tacacaagcc tcgaaaccag cccactcagt aattcgatta
    22261 tttaatttag tcagcataca ccacctcctt aaactttccc tattttacgc taaggattta
    22321 accaatggca aataatttag ctatcggaat ggtaattggt gcaagcctat catcaggttt
    22381 tcacggtgct ttttcaggag caaagaaaaa gctagaacaa ttagaacagg ctgtcgctaa
    22441 aaatcagcaa agccattcta aattaggttc tgagcttact gcattgcgta gtaaacaagc
    22501 tactctctat gcagaaatga gccgagccag tagtaaaggt ggtgctgggc ttgctcttat
    22561 gcaacaggaa tataaaaaaa ttggcaaaga aattgccaat attagccgag agcaacgcaa
    22621 atggagtaca gaattagaaa aatctattgc taagcaaact aaattacgca atgcaatgga
    22681 acgtcataca caagctaaag aaaaccgaga cgagcttaaa gggaaaataa ttggaacggt
    22741 agcttctgcg gcaatgggtg tcggcgtgat gaaaacctat atggagcaag aagaagctgc
    22801 gaataatctt aaaatttcaa tgctgaaagc agatggttct ttcgggaaat ttgaagagat
    22861 tggcaagatt gctgacacat tagggacaga tttaccggga acaaaaaaag atttctacaa
    22921 gctcgcaatg gcattaaaga aacaaggtgt ttctgatgat gtgctgaccg gaggggcatt
    22981 aaaaacttct gccgaactga atgtcttgct tgatatggat caggaaagtg gtggagagtt
    23041 tttagcgaag tttatggaat ctcaccggct caatgaaaat gagttaccac aagcagccga
    23101 ttatctgcaa cgagcaatgt ttgccggcgg tttaagtaaa gagcagatgt atgaatcgat
    23161 gaaatactat gctccaaaac tgaactcaat gaaacttaca ggggctgaga ataccgaaaa
    23221 agtgctggcg attgaagcaa tggcaggtca gcaaggctta gagggttcaa cctttggtac
    23281 gggcttaaat atgatgttaa gccgtatgaa taaaggaccg gaaatgctca aggcagcagc
    23341 caaaggaatg aaagccgaag ctcgagagat gatggaggct gtcggagtag aatttaattt
    23401 ctgggataaa aacggctcat tcaaaggcat tgatggaatg ctggctgaaa tgacgaaatt
    23461 tgaaaaaatc cgcaaaaaat tcggtgacaa aggtgtaggt ttagttgcag aagagctgtt
    23521 tggcattgaa ggtgggcgtc ttgctgatat tttggctatg aaaggtcaaa aaggcttaga
    23581 tgaaatgctg agtaaaatgc gtgaacaagc cagccttcaa gatcgcatta accttaaaac
    23641 aaatacatta ggtgctgccc tagaatcttt aggaggagcg tgggaaacct ttgtggggga
    23701 tattggctct gtctttgccg aagatataaa agggttttca aaaggcttgc aatctgcggt
    23761 ggaatggctt gtgccatttg tatcaaaaca taaagaggcg attaaatttg taggtggttt
    23821 ggttgctgga cttgttattt tacgaggcgt tttacaagct ggttcttttg ttattcaaac
    23881 ggtaactatg gcaatgaacg ccctcaaaat tgcagcaatg accaatccta ttgtcttagt
    23941 cattggagca atcgcagcgg gtgcttactt gatttatagc cattgggata gcattgcccc
    24001 ttggtttgca aatctttggc aaaatgtgac cgcttacttc aatcaatttt ggacttgggt
    24061 aaaaggtgtt tggtcgggcg tgacggactg ggtgtcttct gtgtggaatg gtgtagccac
    24121 ttttttcggg cagttatgga ctacgattac tagcttattt agtgtagctt ggacaaatat
    24181 ccaaacaata tggggaggag caacagcttg gttctctggt ttatgggatg ggattaagtc
    24241 tgcggctttt ggtatgttcg acagtattag ccaaaaagtc agctctgttg tagcaagcat
    24301 taagagcttt ttaggctttg gcgatgatgt ggataaaatg ggggcaaaaa ttggcaatgt
    24361 agctacccaa gtcatcagca aagaacactc aggacaactc agcaaaaccg cagatatggc
    24421 agccaataca gggttctcta gcggtggttt caccgggcaa ggcggtaaat atacccctgc
    24481 cggtattgtg cataagggcg aatatgtgat gacgaaagaa gcgacctctc gtttgggtgt
    24541 tggaatgctg gatagcttaa actatggcgg tgctatgggt ttcactcaac ctgttttgca
    24601 accgatttta ggtaagcaaa aaggcttttt tggctcactt tgggacgatg tgaaattcgg
    24661 ggcaaacttt gtcggcaatc tgcttggctt aaatcaaggc agcctgaaaa cgcctgattt
    24721 taacccaaat gcgaccaatg caagcggtca aaattcgtcg atttttagca attatgaacc
    24781 actcaaccga aatgcggtaa cacagcagga aagctcgcaa gcaccgcaga tagtcgtgaa
    24841 ctttaacccg acaatcaatg taaacggtaa tgctacgcaa gaggctcaat cgcaaattat
    24901 gcagcagttg caaatgagtg cttatgagtt tgagaaattg ctcaatcgtg tgctagacca
    24961 acgaagccgt agagcctatt aggagtgatt tatgtatttt atgttaggca atattgcctt
    25021 tgagccggtg aatttaactg aatttagcga gcaacatacc gccgaatttg ccgaacacgc
    25081 tgtgctaaaa ggcaaacctc gtttacaagc gatgggcgaa aagctaaatg aacttaattt
    25141 ttctcttcgg ttgcaccata ccataggcgg tgtagagagc cgttatcaag ccttgcttga
    25201 agccaaagcg aagcaagcgg cattggcgtt aatttggggc agtcgttata agggagatta
    25261 tgtaattgtc gatatttcat cgaccacatt gttcaccgat ggcagaggca atgccctcgc
    25321 tcgtgaaatg caaatcagcc tgaaagagtt tgtgggcgat aatgacggtg gtgtgttggg
    25381 tgaggcgtta aattttggtg gtggctcact gcttggctct attttgccaa gcggtgcggt
    25441 aactacccta tctcaaatca aaacagcagt cagccgaggt gtggagcttt ataacagcgg
    25501 caaacgctta gttgacgaag tgcaaaatac cgtggcgatt attcggcagt tgaaaaatga
    25561 cccggcaact gcattggcat atttacccgc tgcattagcg aatttagacg gggcattggg
    25621 gcattttggt aatttgaccg gctcatcggc gaccttgggc ggtttgcagg agaaactacc
    25681 ggcagcagtg gtctttagcg aggaaatcgg cgagatttat cagagtttgc aaacaatgcg
    25741 aagcagtttg ggtaatgcct cggcaaatag ctgggataat tggtttacac cggcagagag
    25801 tgcattgaca gaggtcacag acagctttga ccatctagca aaaccggtgg caaaaatgac
    25861 cgcttggatt gtgctgcgta ccgatgagga ggcagaaaat gacacagttg cttaaacata
    25921 tcgtcaaaca gggtgagcgt tgggataact tgagctatca atactacggc aatgcgtttg
    25981 aatatggtcg aattattgat gccaatccgc atatcggttt ttgcgaggtg ttgccgatag
    26041 gggtaacaat ttatatccct gtacttaacg ttaagccaac gaataatgaa aatatgccac
    26101 cgtggctaag ggggcgtgat gcgtaatgta ccgaagcccg atttttcact gttttatgaa
    26161 aaaaccaata tcactgccga gattgagcca catttggtgc aactgaccta tatcgaccat
    26221 ttggaaggtc aatcggacga actcactgtg gaatttgaag acattcaagg caaatggata
    26281 cgccaatggt tcccgactca gggggataag ctcaaagcgg cgattggcta tcagggcgaa
    26341 tttctgacgg acatcggtga gtttgaaatt gatgaagtgg aatatagtta taagccttcc
    26401 actattaact taaaagctct aagcaccggt atcagcaaag ccaaccgtac tttaaaaccg
    26461 aaagcctatg agaatacgac tcttgctcag gtggtggcaa aggtagcaga taacctgaaa
    26521 ttaaaggtag tcggtaagat taaggctatc ccgattaaac ggatcaccca atatcaagag
    26581 cgggacgtgg agtttttggc acgccttgcc cgagagtatc atcacagctt taaaatcgtg
    26641 ggcaatcagc tggtctttac cgataaaagc gaactggggc aaagtgagcc ggtgttaatg
    26701 ttggaagaac gggatactat ttccctacgt ttgcgagatc ggattaaaga cactgcaaag
    26761 gcagtggaaa tcagcggcta tgatacaagc ggtaaaaaag tggtgaaaaa acgcaaaaag
    26821 gcaaaagcct tacgcccgaa tataaaacaa gctaaatcag ccagtgaaga tacgctgaaa
    26881 atcatcacac gaggcgaaag tcaagaacag attgatgcac ggggtgaggc tgcgttggct
    26941 acgcaaaatg aagatcaggc ggctggtgat attgagctta tcggcaatcc taaattggtt
    27001 gccggagcaa cgattttatt gcgtaacttt ggcgtgttta gtggcaagta tctgattaaa
    27061 tcatcacgcc atacgattag ccgtagcggt ggttatacta ctcatattga ggtcagaatg
    27121 ttggagttta ttgccgatga tttaatcaca ttaggtatgg aggctggcaa tgcaaacgca
    27181 taattttggg gcgacttatc aagaagggat tgtatccgct gttgatccga aaacgcacaa
    27241 agtgcggtgt aaaatccctg cgctggaaga tttagaaact gcttggctgt cgtttttgac
    27301 cccgaacgcc ggtggcaatc agttttactg tttgcccgat gtgggcgagc tggtggcaat
    27361 gctacttgat gcacaaggcg aaggtggctg tgtgtttggg gcaatttata acgagcaaga
    27421 cccgacaccg gttgccgata gcgatatgtg ggttaagaaa ttcagcaatg gcacggagat
    27481 ttcgcataat cgcaaaaccg gcgatgtggt agtgaagact aacgggcatt taaccgcaac
    27541 ggctcaaggt ggggctacga ttaatgcaaa caccgtagtc aatggcacat tgcacgctac
    27601 cggtaaaatc acctctgacg aagaagtctc tgctcctgtt gtgaaacaag gcacggtaga
    27661 gcttggcaca cataaacacg gcggtagccc tgaacctaat aagtaattct ttaaaccagt
    27721 ttaaaatccc ttttaaagat agccctgtat catcagggct atgaacagac aaacttccat
    27781 tcaattaacc cactggcaac ttgcaccctc aaccgataca gttgaggtgg tgcaaggtat
    27841 tgatgatatt catctttgta ttgtcaatat cctttcaacc caaaaaggca cggatatttt
    27901 acgccctgaa tttggctctg accactttcg ctacattgac taccctgaag atgtggctat
    27961 cccgaatttt gtacgggaaa ttacaaccgc cttgcaaaaa tgggaaaaac gcattgaggt
    28021 tgaagaagta ttggtggacg gttcagctcc gcattttatt tttactgtga tgtggcactt
    28081 aaccgatgat gtttatcgtg aaatttaccg aacaacggta taaggatagg caatgacaga
    28141 agcgattaaa atcattgatg acgatgtaaa aaccgtactt gccgaagcga ttgccgatta
    28201 tgaaaaacgc accggtaaaa tcttacaacc tgcacatatt gagcgttcca ttattcaaac
    28261 ctacgcttac cgtgagcagt tattacgcaa aggcattaat cacgcctttt tacaaacctt
    28321 tccgcagttt gcgacgggga ttgcgttgga tttgtgcggg gaaacttttg gttgttatcg
    28381 gcttagagat aaacctgccc gtactttact acgcttttca gtaagtggtg aacatacagc
    28441 tatagttatc cctaaaggaa cgcaggtggc aattaatgac gagctatact tttcaacgct
    28501 caatgacgat gttattactc cattaattac ctatgtggaa attgatgcag agtgtaatca
    28561 ggttggtgtt atcggtaatg gttgggaatc aggaagagtc agtaaactaa aaacagcact
    28621 ggcaactaat tataaagtaa cagttgaaaa cattgatatt acgagcggtg ggttgatgca
    28681 ggaagaagat gaaccttacc gtaagcgaat tttagccgca ccggaagcct ttagtaactg
    28741 tggctctatc gctgcatatg attaccacgt ccgctctgta tcccaagata ttgcagatgt
    28801 gaatatctct acgcctaggg gtggcttggt aagaattgcg gtattgaccc aaaaagggtt
    28861 accggatacc cgattacttg atgatattaa aaaacacgtt agtgccgaac ggctacgccc
    28921 actttgcgat atggtcgagg ttattaagcc cacagaacgc cgttatcaaa ttacggcacg
    28981 tttaatattg ttagatggct accgtgagga tttggtaaaa gcaaaagccc gagaggcatt
    29041 acaactctat ttgtcagaca agactaaaaa actcggttta gacattgtgc cgtcggccat
    29101 catttcagct ttaagagttg agggtgttta cgatgtgaat ttaattcagc cagtcaaaac
    29161 tattatcaac gaaacagaat gggcgaactg cacagcaatc agcgtagaag tcgaagagga
    29221 gcgtagagat ggctagcttg cactatgcgg acattattgt gcgagaccct aaatataaag
    29281 cattagcgga tttaagcaag cggttaaatt tgctcgatac ttcgcaaatt atgactacgc
    29341 tggtggattt attgggcgac gaatgtatcc cattgctggc agaaaaatgg tcggtaaccg
    29401 gctatgacgg cttattggtt gcagatagcg aaaactcaaa gcgagcctta attaaaagag
    29461 caatcgaact acatcaacta aaaggcacgc cttggtcaat tcgtgaagta ttacgcagac
    29521 taggtttcgg agaggttgag attgacgagg gactgaaaaa tcggctgtat gaaagcactt
    29581 atgtgagcgg aatccccgaa aacgaacgct gggcgcacta tgcgattagg ttgagtaacc
    29641 cgattaccaa tgagcaagct cgcaatatcc gtaaggtctt acgcaatttt gctccggctc
    29701 gttgctctct tgcagttttg gattataaag cagatccaat tcgatataac aataaagcaa
    29761 cctacaacgg cacatataac cacggttcaa gttaaggata aattatggca ggtgtacaag
    29821 aaaccgcaca gtgggaaaac cacgtttaca gaattgaaga aaatgaccca gtccacggag
    29881 gagaaaacgg tgtaaccaat aagccaatta agcacctagc aaaccgcacg ctctatctgc
    29941 gtagattgct caccgaggca ggacaacgca ttaacccgaa aaaaatcaca gctaccagca
    30001 aaaatagtaa tgatttaact gggcatactc acgagattga ccacgcaagc ctaacgcaaa
    30061 aaggcattgt gcagttaaat gcgagtatcc aatcaactag cacaactgag tctgcaaccc
    30121 cttcagcagt taaagcagcc tatgataaag cagcaacagc agaacaaaaa gcgacattgg
    30181 caaatgagaa agtcgaggcg attaaagtag gcggacgaaa cctcctcatt caatccaaca
    30241 ctccacacaa cgggaatgcg tactcagtca gatacgcatt ggctgaggct ccgaaagtag
    30301 gcgaagagat tgtagtcact ctatggggtg aactcggctc tcaacgtact ggaattggcg
    30361 tttacaactc tcaaggtgct acggagttgg caaaactggt caaaattcgt gatggagttt
    30421 atcaaggtaa aggcatttgg cgtaagccga tgagtgaggg acgagagcga acgcctaacg
    30481 acacgcacct gaatgtgtat ttttacccga aaaccgcaac agataataac cgtattgagc
    30541 gaatccagct tgagcgaggc tcaatcggta cagattggac gccaaacgag gcagacttaa
    30601 aggcagagat tagtgcaatc cgcactaaac taatccctgt acggggtggc aatgttgcta
    30661 caaccttaaa tgcggctaag cggtcggatt ggtttagaga aatgcaaatc ccttatcaaa
    30721 gtggttgggg gtattacacc caagcatttc ataactcagg gacgcaacac aactacagca
    30781 atttaccgct gacaggtcgt tattatttta tcgccgaact ctatcataac tctggttatt
    30841 ctcacttgcg tatcacctat ccaagtttaa aacgcacctt tgagagtgct atcaacctta
    30901 acaatgagga tttgattcta gattggcgtg aggtggtgtt tttaaaagat ggattgtatc
    30961 aaggcacttt taaaacaaca ggcacactag agtctgctta tcgtctaaga gcggctcgtg
    31021 atgatgaacg ttatcagcca tacctgcaaa tggtcgatat gggctatgac cttgccaatc
    31081 ccccggtagg aaaaaacaaa attattggca gtgttgatta ttacgttaaa aacggcacaa
    31141 gcgagtcttc caaagcgacg atgatggcag cggcaatgcc tgacaagaac gtattttggg
    31201 aagtagcatt gtggaatgca gcacagcaac ggaatgtatt gtttaagggg tatagcaaga
    31261 ctaacaattt gagtattggt aaaaccaccg acaatgagcg agaccgagta caagtcaatg
    31321 gcacaatcca agccaccgca ccggctgata acgccaataa cgaccaagtg ccgactacgg
    31381 cttgggtgcg taagatggca aagattttag ctgatggcaa ggtgagtaaa gccggtgaca
    31441 caatgactgg gcatttattc atcaagaatg gggaatactc tgcggtacat acctataaca
    31501 caagtggttg gttcgcaaaa tgggaggcgg ccccattatc tgattcgcac tttgctgcca
    31561 ttgtatatgc caacgacaga gagacagtta tcaatcgggt gttaattccc aagaagaatg
    31621 gcacagtcgc tttaattaat gatattgccg ataccgttag agccgctgtg agtcgtagtg
    31681 ataaatcccg tagtagaact tttcctctgc aaacggggaa aaaatataca accacaggga
    31741 gcgttacgat ttatccagat ggccgaattg tgcagatttt ccacttaaaa aatattaaag
    31801 cggcgtggtt tgattatgag gcaagtgcgg taggagatag gcatagagtt gtcaatatcc
    31861 ctctttggac tgcgatgcca aataaaattt ttgatgtcga tgttaaaact gttcgcccct
    31921 caaataatgc ctcaacttac tacatagaag cggctgaatg gctgagtgcg tggcagattt
    31981 acggtaatga cacgaacaaa tcaagtgtca atattaatct atctcgattc agaggtggaa
    32041 aggatgaaga tatggacttg tatgtaacag ttgagggata ttaaatggca tattttgtga
    32101 atgtgattga tgagcaaggc aattatgagc taattgatga tgagtttatt gggctgtatc
    32161 cggatttgga ttttaacaca ttacccaaac tcacggataa gcaatatcag acctaccttg
    32221 ccaaagcaaa cggcaaagag cgatttgtaa atggcgaatt tgtgtatgag caaattaagg
    32281 tggatatgca agcggtcatt tccgctgaaa aatctgcaaa gttagcagag attaaccaaa
    32341 aagcacaagc ctttatcaat gatttagcca aatacaacga aacgcctaag tttgagcgtg
    32401 atacttggct tgagcaagcg aaagaggcaa aagcgtgggt ggctgaccca accgtacaaa
    32461 cgccaaccct tgagttaatt gcgcaaatgc gaggcatacc gctcgacacg ctacgccaaa
    32521 aagcctacga aaaagcgatg gcgtatcaaa cggtggctgc tattgtagca gggcaacggc
    32581 aaggatacga agaccgccta gaacaagcgg aaaccttaga gcaaatacag gcgattgagc
    32641 cgatgtatca attaccgcaa ggagcaaaca atgagcaaac tgactaaatt aaaatcgtgg
    32701 agctaccacg tcttaattgc aatagatcaa ctctttaacg ctctcaccgg tggagcagcg
    32761 gacgaaacat tttctagccg ttgctaccgt ggtgcagtgc ttgcggaaaa tccgaaaaaa
    32821 cgttggcgtt tttggcactc ttttttgaat tgcttatttt tagacccaaa gcactgctac
    32881 gccgcttata aagcggaggt acaccgcaag cagtatccga aggcatttag acttgggggg
    32941 atttaatgaa aaagcaaaac ttaaaattag tgcggggcga tgatgtgagt tttagattca
    33001 gcattaaaga cagcaccgga caacctcttg cgttaaagga tttgtggttt gatttgcact
    33061 tagtcagcta tcaaggtgag gtgctgattg ctctctctac tgatgatggc agtattgaga
    33121 cagcagaggc tggtcagatg gtgttacatt ttagtcacca gcaaactgct cgagctgaat
    33181 ggacaagtgc caattatgac ttgcaactca cgcacgacta tacaagagtc taggaggtgg
    33241 agatgacctc tatcattgaa ctagacctga ctgatgacct catcattgag gtgcagttag
    33301 atagcacaat cattaccggc gatagctttt tagccaacct gccggattta ctccaaattt
    33361 acaaccaagc aaagtcagat agcagtaagg agcttaacaa tgactaaaca caaaaaccac
    33421 gaaaaccacc cgattgctat aacattctac caacttggca aagatgtcgg cgacattaac
    33481 cgccggctag cggttattga gcaacgtgag caatcagcac agccaagcca accacaagct
    33541 gaggcggtta taatgccaca gcaagatgct gttgccatta ataacatcaa tgccgttttg
    33601 gaggcggtcg aaaaatacaa aacttttgaa aaatacaaca ctcagtggca agagcgtttt
    33661 gaaaaattcc tgtctcgtaa cccgaatgat tacgagttaa gtgaagagca acgccaaaaa
    33721 ctttaccaca cgatgtgcca gcatttgtgg caggtgaact ttactgaaat caatagtaaa
    33781 cagtatgaat taccgatccc gcaacaattc cagggtaagt ttggctatat tgtcaaaacc
    33841 tcgggcggta gcacagaaat tagttttgag ttgctatctg aaactcacag cacttatctt
    33901 tatccggata gctatattcc cgagggcggt ggcgatgtaa tgctcaagcc ggcttatatt
    33961 tatttagtga ttccggactt agtggcaatg cgtgagccgg aggtggtggg ggtttcaccg
    34021 ctgaaactga acattctgca gaaacacctg ctgacacctc agaaactaca ggagcagaag
    34081 cccttgaaca cgaaggcaaa ctgattgatg gcgtatggca ttttgagcga gagccggtaa
    34141 atccaccgag agatcagaat tattatgtgg caggtaacct gttctacaac ggcataccgg
    34201 attgataaat tgatgtttca gtaaacctaa ttagggggga atcccccttt tttattatta
    34261 taaggaaaga aaatgaataa atctttagta gcgttagctg ttgcagcgat ggcatcgaaa
    34321 gcacaaacta aa
//
LOCUS       DANLRK010000005        34162 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.005, whole genome shotgun sequence.
ACCESSION   DANLRK010000005 DANLRK010000000
VERSION     DANLRK010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 34162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34162
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.005"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(92..1225)
                     /locus_tag="PXA33_000166"
     CDS             complement(92..1225)
                     /locus_tag="PXA33_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012478416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis PLP-dependent protein"
                     /protein_id="HDL5119550.1"
                     /translation="MTKFNNIETTLVQIGNRTDPRTGAVAMPIILSTAYGRDGLGEST
                     GWDYTRTKNPTRAVLEQGIADLEGGDAGFAMASGMAAIQLVMSLFKAPDEWIISSDVY
                     GGSYRLFDFSHKNHGTLKPVYVNTADLAAIEAAITPNTKAIFVETPSNPLMEECDVAA
                     IAKIAKKHNLMLIVDNTFLTPALFRPIEHGADIVIHSATKYLSGHNDVLAGLIVAKDS
                     DATKVNGQNLTERLFYFQNCAGAVLSPFDSYLAVRGMKTLALRMERHQSNATELAKFL
                     AEQPEIDSVLYSGKSGMLSFRLQKEEWVAKFLKAIKLITFAESLGGAESFITYPATQT
                     HMDIPEAERITRGITNNLLRFSVGLEHVEDIKADLLQAFKQLK"
     gene            complement(1242..2054)
                     /gene="dapB"
                     /locus_tag="PXA33_000167"
     CDS             complement(1242..2054)
                     /gene="dapB"
                     /locus_tag="PXA33_000167"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005696006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HDL5119551.1"
                     /translation="MTLKIGIVGAGGRMGRQLIQAVHNAQGVELGSAFERKGSSLVGS
                     DAGEVAGVGTLGVVISDDVAQNVAGFDLLIDFTRPEGTLEHLDICVAHHKQMVIGTTG
                     FDDAGKAAIKAASEKIGIVFASNYSVGVNLVFKLLEKAAKVMGDYCDIEVIEAHHRHK
                     VDAPSGTALSMGEHIAKTLGRDLKTHGVFERNGITGERKRDEIGFATIRAGDVVGEHS
                     VWFADEGERVEIAHKASSRMTFANGAVRAAKWLNGKQNGLFDMTDVLDLNNL"
     gene            complement(2105..2482)
                     /locus_tag="PXA33_000168"
     CDS             complement(2105..2482)
                     /locus_tag="PXA33_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5119552.1"
                     /translation="MGIKVLFGTKISAELQYLPEKDLVKILQFKQHVEIYGLENLAGR
                     NKSSDDVPTNDPYWAEKVAKAQKYSLWHYHIGIPEYDTSQGFGNYTSEYILHYVKGEN
                     FIQIVDFTSHPPFKLPSESYLQN"
     gene            complement(2472..2945)
                     /locus_tag="PXA33_000169"
     CDS             complement(2472..2945)
                     /locus_tag="PXA33_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5119553.1"
                     /translation="MMKNLPLSMADTLAVIYPAFDFAADDKPATEQQISLLNFRITMA
                     NQLLIACIETIEKNPNFWAEIDLNDFKKYLNQNKVVIFDTQQYFTDGSRTMAILDNMK
                     SNIRKIEQQLSQATAFNFDLERMKERLNDTFHEVPKDITNVNEFRNWLKGVANGH"
     gene            complement(3013..4101)
                     /gene="rlmM"
                     /locus_tag="PXA33_000170"
     CDS             complement(3013..4101)
                     /gene="rlmM"
                     /locus_tag="PXA33_000170"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015432676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HDL5119554.1"
                     /translation="MFNKLALYCRAGFEKEVAGEITDKAAQQGIFGFANLKENSGYVI
                     FECYQAGEADRLAQALKFEQLIFVRQMLVVGDLLQNLSENDRISPILAQYAELNPRQS
                     SDVFVETPDTNEAKELLTFCRKFTVPLRSALKKQGWLGKSENMQGSVSLHILFTRPGA
                     CYVGYAYNHNKSPFFMGIPRLKFPAEAPSRSTLKLEEAILTFIPQAEEKKRFNDTMVG
                     VDLGACPGGWTYQLVKRGVFVYAVDHGKMAASLHETGRIEHCPEDGFKFQPPKRKQID
                     WLVCDMVEQPIRISKLIAKWLTNGWCRETIFNLKLPMKKRYQEVQLCLNFLEEELTKH
                     GFWFKMQAKHLYHDREEITVHIAVMGQK"
     gene            complement(4101..4493)
                     /locus_tag="PXA33_000171"
     CDS             complement(4101..4493)
                     /locus_tag="PXA33_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HDL5119555.1"
                     /translation="MKNKFVAIAAFSGLFTVAFGAIASHALSAQLSPKALGWIETGIK
                     YQMFHTLAILALGLFQIANFGQNPPVCRAKAFNIIGSNWALGILLFSGNLYLLALNVP
                     TIHWLTPIGGLCFMLGWIALIYISVRKA"
     gene            complement(4600..6001)
                     /locus_tag="PXA33_000172"
                     /pseudo
     CDS             complement(4600..6001)
                     /locus_tag="PXA33_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252330.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
     gene            complement(6156..8513)
                     /locus_tag="PXA33_000173"
     CDS             complement(6156..8513)
                     /locus_tag="PXA33_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin guanine dinucleotide-containing
                     S/N-oxide reductase"
                     /protein_id="HDL5119556.1"
                     /translation="MNTALEKGKFVFLDKPKNTITTAAHWGALNVTVENGKVVKSEGS
                     LAKNHQNELQDVVADQLYSPVRVKYPMVRKGYLEGNKDTTLRGRDKWVRISWDKAFEL
                     VSGEIQRVREQYGSESIFGGSYGWYSSGSLHASRTLLQRYLNVTGGFTNTKGDYSTGA
                     VQVIMPYVLGNIEVYAQQTSWHTIVESTELLVIWSSNPLNTLRIAWTSAEPEGIDYLA
                     KLKASGKRIICIDPVKSETCQFLEAEWLPINTGTDVALILGIAHTLITENKHDHDFLT
                     KYTVGFEQFADYVLGKADNLPKTAEWASQICDIPEEVIKTLANDFVTHRTMLMSGWGL
                     QRLQHGEQSHWALVTLAAMLGQIGLPGGGFGLNYRYSTNGLNVNSGVRLGAINAGKPN
                     PDMPFFPVSRIADALLKPNQPYDYNGQTLHYPNIKLVYWAGGNPFAHHPDTNYLVKAW
                     QQPETMIVNECYWTPTARMADIVLPATTSYERNDLTVAGDYVAKHIIPMKQVVEPQFE
                     AKNDYDIFAELAKRAGKEQAFTEGKSEMEWLKEFYQVAFEAGEKANTPLLDFEAFWAE
                     NKVLSFEAEHQNNTFVRYQDYRTDPIKHKLATPSGKIEIFSETIAKMNYEDCKGHPTW
                     FEHHEYSGKTNEAEPLALVTPHIKYRLHSQLAYSSLRQYYEVNGREPMLIHTKDAADR
                     GITSGDIVRIFNKRGQVLAGAVVTDGIIQGTVALYEGGWYDPQDLGKTDTPLCKNGNP
                     NVLTRNEGSSKLGQGNAPNTTTVQVEKYVGDAPEVTVFKAPFFSE"
     gene            complement(8649..11255)
                     /gene="acnB"
                     /locus_tag="PXA33_000174"
     CDS             complement(8649..11255)
                     /gene="acnB"
                     /locus_tag="PXA33_000174"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014391308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HDL5119557.1"
                     /translation="MENFIENYQQQVDERAALGVVPQPLSAEQTAQLVELLKNPPAEQ
                     AVFLLDLFTNRIPAGVDEAAYVKAAFLADITKGVATSPLISPAHAVKLLGTMQGGYNI
                     ETLLSALDNPELAPIAVEALSKTLLMFDNFHDVKERADQGNPFAQQVLESWANADWFT
                     SRPKLAEKLTVTVFKVTGETNTDDLSPAQDAWSRPDIPLHALAMLKNERDGIFPDENG
                     HTGPIKQLEALKEKGFPLAYVGDVVGTGSSRKSATNSVLWFMGEDIPYIPNKRAGGVV
                     LGGKIAPIFFNTLEDAGSLPIEVDVTNLNMGDVIDIYPYAGKICKHNSDEVLAEFELK
                     TEVLLDEVRAGGRIPLIIGRGLTHKARVALGLPESDVFIKPQSVSESNKGYTLAQKMV
                     GRACGVEGVRPGQYCEPRMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAA
                     YPKPVDVITHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIG
                     ISFPAGSGLVAFAAATGVMPLDMPESVLVRFSGTMQPGITLRDLVHAIPYYAIQQGLL
                     TVEKKGKKNIFSGRILEIEGLEDLKIEQAFELSDASAERSAAACTIKLNKEPIIEYLN
                     SNITLLKWMIAEGYDDKRTLERRIANMQAWLDNPELLEADENAEYAAVIEINLDEIKE
                     PIVCVPNDPDDARLLSQVQGDKIDEVFIGSCMTNIGHFRAAGKLLSQAQGELPTKLWI
                     APPTKMDAGLLTEEGYYSIYGKSGARVEMPGCSLCMGNQARVANNANVVSTSTRNFPN
                     RLGQGANVYLASAELAAVSALLGKLPTPQEYLDYMNNLQKDKDDIYRYMNFDQINSYV
                     KKADNVIVQVAV"
     gene            complement(11275..12522)
                     /gene="icd"
                     /locus_tag="PXA33_000175"
     CDS             complement(11275..12522)
                     /gene="icd"
                     /locus_tag="PXA33_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HDL5119558.1"
                     /translation="MQTQVQIPQGETIQLNQDGSLQVPDNPIIPFIEGDGIGVDVTPA
                     MKAVLDAAVEKAYQGKRKIAWMEIYAGEKANKLYGENTWLPDETLELIRQYHVAIKGP
                     LMTPVGGGIRSLNVAMRQGLDLYNCLRPIRYYDGTPSPVKHPELVDMVIFRENSEDIY
                     AGVEWVAGSPEANKVIEFLQQEMGVKKIRFTEDCGIGIKPVSKQGTQRLVRAALQYVI
                     DNNRKSLTLVHKGNIMKFTEGAFKEWGYQVAEEFGAKLIDKGPWMSLTNPKTGEEIII
                     KDSIADAFLQEILLHPTEYDVIATLNLNGDYISDALAAQVGGIGISPGANIGFEAAIF
                     EATHGTAPKIAGQNKGNPGSLILSGEMMLRHLGWVEAADLVVKAVSKTIANKTVTFDF
                     AEMLEGVTLRSTSEFAQDIIANM"
     gene            12756..14036
                     /locus_tag="PXA33_000176"
     CDS             12756..14036
                     /locus_tag="PXA33_000176"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005762304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HDL5119559.1"
                     /translation="MSKATLTLETGEKLELNMQKGTLGYQTIDIQPFLKHKLFAYDPG
                     LVSTAVCRSNITYVDGDEGVLLYRGYPIDQLAKHSDYLEVGYMLLFGERPTKEEYKDF
                     VQLIKKHTLVHEQLTKFFSGFRRDSHPMAVMCGVSGALAAFYHDAIDVNNQAHRELTA
                     IRLLAKIPTLAAMCYKYSIGQPFMFPQNHLSYAGNFLYMMFATPCEPYEVNPVLERAL
                     DRIFILHADHEQNASTSTVRTAASSGANPFACIAAGIASLWGPAHGGANEACINMLKE
                     IGTVDRIPEFIARAKDKNDPFRLMGFGHRVYKNYDPRAKVMRETCHEVLKELNIDNPL
                     FEVALELERIALSDPYFIEHKLYPNVDFYSGIVLKAIGIPTSMFTVMFALARTVGWIA
                     HWKEMYLQGNIKIVRPRQIYTGHTKRDFEPMDKS"
     gene            complement(14109..15338)
                     /locus_tag="PXA33_000177"
     CDS             complement(14109..15338)
                     /locus_tag="PXA33_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5119560.1"
                     /translation="MFQAEPKNGKVTVEVLKQYLIDELKIDEEEIAIVIGNQHELDNI
                     DLFNPQCKINYIITVEALKEGWDCPFAYIFCSVQNVSSSKEAEQLLGRVLRMPYAKHR
                     QIEDLNRAYAPLSSKSFGETARNLQDKLIGMGFEALEVAEMLRIPQQENWIGNEGDLF
                     YTPTTVLALTQLPEMSDLSEAKRSQLKITEQEGHILVQVKGNLSEKLEKALIRTATGK
                     KKEQIEQQLQIHQNRVEILSSPAEKGEIFKVIPQLCMNIQGEFDLADSQVLLDYQSWN
                     LLDYPAKLDGFRLQENSQSFEIDVEDKRVSYHFNQQQLSFSDDWLRNPVKRGFSLPLA
                     HQNFYPDFIVELIDGRILIVEYKGEQLKSNEDSKEKALIGNLWEKLSQGKGLFIMAVK
                     SDENGQDVRTQLLNKLA"
     gene            complement(15305..16294)
                     /locus_tag="PXA33_000178"
     CDS             complement(15305..16294)
                     /locus_tag="PXA33_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDL5119561.1"
                     /translation="MKLKNYQNEALNRVSHYFSACLQSDAKTAFSQIQPEYSYKIPSE
                     HSNLRDVPYVCVRIPTGGGKTLLASHSIARIAKQYLDCDFPVTLWPVPSRTIKTQTTE
                     ALKNPQHPYRAVLDKTFNREVMVIESEDFDLLRPQDFGNKAIVIVSTIQNFRVENQDG
                     RKIYAFNEKLTAHFERLLPHIQANLEKISTTDLQENGLTSKQTGKIKCSFANLLRAYQ
                     PLVIVDEAHNARTSLSFDVLSQLSPSAILEFTATPNTDRKAGSNVLYHVSASHLKAEE
                     MIKLPIVLTEHHNWQQAIDGAVINRGALAQKAQYESEYVRPIYCFKLSRKTAK"
     gene            complement(16310..16828)
                     /locus_tag="PXA33_000179"
     CDS             complement(16310..16828)
                     /locus_tag="PXA33_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HDL5119562.1"
                     /translation="MNRRYIGIEIGEHAKTHVVPRLKKVIEGEQGGISKAVNWQGGGS
                     FRFCELGEEVFDAFGSLNPNIRFDDLAAHIWYLENHFPLQKNSEKSPLVGTFNGKAYY
                     LLYNGILGDKRPQSGNVLTRKLMAELPYFAEFIQQGMDIVVYGEACRLGEVQLAEKKI
                     TFKQIPYDVMSR"
     gene            complement(16838..16909)
                     /locus_tag="PXA33_000180"
                     /pseudo
     CDS             complement(16838..16909)
                     /locus_tag="PXA33_000180"
                     /inference="COORDINATES: protein motif:HMM:NF013704.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(16916..18025)
                     /locus_tag="PXA33_000181"
     CDS             complement(16916..18025)
                     /locus_tag="PXA33_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HDL5119563.1"
                     /translation="MTTLTWYGKEEAVKKAKQTPYCLLRELPDFSHNIARQPTADSRQ
                     PTADSRQPTADSRQPTADSSASAETLSKVKQNLIIQGDNLSALKSLLPFYAGQVKCIF
                     IDPPYNTKSAFTHYDDNLEHSIWLSMMYPRLEILRELLAEDGSIWITLDDNESHYLKI
                     MLDEIFGRKNFISNLIWEKKRKPSFLGKVGKVTDNLFCYAKNKNELNELNDLTYLETS
                     GDETYPLYNAGNSREILVFPPRSIKFKKHSDGLYKPTIFSKKTSSITLISELIIEDQW
                     NKNTVEIEGEWRYSQKSIDEQLKNGDYYIIKSLDFRPRRVFERSAEKKKIHNLISRAH
                     YDMATNEDSASEHLELFGEDIAEDSFDYPKPEKLI"
     gene            complement(18166..20652)
                     /gene="torA"
                     /locus_tag="PXA33_000182"
     CDS             complement(18166..20652)
                     /gene="torA"
                     /locus_tag="PXA33_000182"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HDL5119564.1"
                     /translation="MKKQDNIDVSRRGFLKNSSLSLAAGAGLASGTTGVVGALASTSA
                     KAAEMKTVVTAAHWGPLGVVVENGKVVKSDSVFEISVPNELQHVVADQVHGETRVKYP
                     MVRKGYLEGNKDTTLRGRDEWVRVSWDKAFELVANEMKRVREAHGANAIFGGSYGWYS
                     SGALHASRTLLQRYLNVTGGFVGVKGDYSTGAAQVIMPHVLGTIEVYEQQTSWEVILE
                     SSDIVVLWSANPLNTLRIAWTSTDQQGIEYFKKLKESGKRIICIDPVKSESCEFLGAE
                     WVPVNTGTDVALMLGMAHTLVAEGKHDQAFLKKYTTGFEKFEEYLLGKTDNQPKDAKW
                     ASQICGVPEETIKKLATDFQSKRTMLMAGWGIQRQRHGEQGHWMLVTLAAMLGQIGLP
                     GGGFGLSYHYSNGGVPSATGGILGSITANPAVEAGAKTWLDETSKSSFPVARISDALL
                     NPGKTIDYNGTQITYPDIKIVYWAGGNPFTHHQDTNTLVKAWQKPDTIIVNECHWTPT
                     ARMSDIVLPATTSYERNDLTMSGDYSMMNIFPMKQVVEPQFEAKNDYDIFAELAKRAG
                     KEAEFTEGKSEMDWLQSFYQTAFEAARKNRVIMPKFEKFWQENKPLSFKASDKAKKWV
                     RYEEFRNDPLLNPLGTPSGKIEIFSEVIAKMNYDDCKGHPTWMEHEEYSGKTTAEEPL
                     ALVTPHSKWRLHSQLAYSSLRKQYAVNDREPVLIHTEDAAARGITSGDIVRIFNKRGQ
                     VLAGAVVTDGIIKGTLALHEGAWYDPLDLGTSEQPLCKNGNPNVLTRDEGTSKLAQGN
                     SPNTATVQVEKFTGQAPEVTVFKEPKYTQG"
     regulatory      complement(20671..20867)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            complement(20871..21986)
                     /locus_tag="PXA33_000183"
     CDS             complement(20871..21986)
                     /locus_tag="PXA33_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HDL5119565.1"
                     /translation="MAKMKKIAVFVGIMALGAAVLGGTQYAMKATSSTEFCVSCHSMT
                     HPQAEWEGSVHFSNRKGIRAECSDCHLPQDGLHYVKAKIIALKDVWHTVVTNKLPDQE
                     AYEAHRLEMAQRVWAEMKENDSMTCKSCHSFDAMVLSEQTEAAQKMHKLAQETNQTCI
                     DCHKGIAHFMPDVPVDNAAAMGELAKHGAEFSTNDKTLYTLAMTPVQLAQGGEIRLLP
                     YAELHQWTAEGEQVKATVNGWQQEGAESVVYMDLGKRIMVALLPDDAQSKVNVTKTVF
                     DNVTNSNWKEINFEITAPKNAVTANITALNHFGDNLNQTHCSGCHAPIGAEHYTANQW
                     IGVVNSMKDRTSMSADDVRAVTIYLQRNAKDVAGSSH"
     gene            complement(22128..23879)
                     /locus_tag="PXA33_000184"
     CDS             complement(22128..23879)
                     /locus_tag="PXA33_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL5119566.1"
                     /translation="MDKRSRWSCLVNEQGEISEWEDKAGADYFAELPQICHYAQALLY
                     SDKNSLQFYYSSNHQWLKISLERHQSFVLTQAEHTDLPYGLSKREIELLTLISSGLSN
                     EDIANELCISKRTVDKHIENIFAKTHTKSRTLLAVFAITEHLICLPTPGKLTQSILAS
                     FNIEQLALQIIAKKQPLVTASTRHEPPIFTINHHHSRPIIIGVPYVEQGIGQIDTREL
                     LNGTQLAVETINQQGGIHGREVQVATAGFYVKDKASILQAYHHLFDQEVDAISTAYAC
                     YLPEVHELIATKGIPYLHIATHSGSNKLAQNLPNQRIENMFQVCASDVTYGLGISHFL
                     QYYRYHYAPWLQNKTVMVVTVSWQKVDIGIESLTLSLREQGWNVDYLELTQEQDNYAT
                     LMAQVHNLAPSIIVFASYFAEDIVQFYQTFIRNPTNAIIYSIYAPSAFLPHEKLCEGV
                     LWSTNSGLSENYLGRQFSQHYERFFGKSPTYSQASIAYDQANILANAWKQSVSPRHFK
                     AVSNAIRLQPHYGVNGTYYFNTDSQIGLTYAETHDLSISLPQLVYQIQQGQSRVIAPE
                     LFANAQFILPPWFSEKA"
     regulatory      24043..24199
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            24188..25213
                     /gene="moaA"
                     /locus_tag="PXA33_000185"
     CDS             24188..25213
                     /gene="moaA"
                     /locus_tag="PXA33_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HDL5119567.1"
                     /translation="MQSIIPIKNVGDDVVSGLVDRFQRQYTYLRLSITDVCNFRCNYC
                     LPDGYKPPSHKQTFLSVDEIQRVAKAFANLGTEKIRITGGEPTLRKDFLEIAHTISQT
                     PGIRKVALTTNGYRLERDVELWQQAGITDINVSVDSLDPRQFQLITGENKLQSILKGI
                     DKAFEIGYPKIKVNAVLMKQYTAPELDKFLTWIKDKPIQMRFIELMETGEMDSFFQAQ
                     HLSGQSVMTRLLQEGWQLQPKAMSDGPAKVLSHPDFKGEIGLIMPYEKNFCASCNRLR
                     VSALGKLHLCLFGEEGIDIRDLLASDEQQLQLETRLKSSLQGKREHHYLHIGDSGVRN
                     NLASIGG"
     gene            25714..26190
                     /gene="moaC"
                     /locus_tag="PXA33_000186"
     CDS             25714..26190
                     /gene="moaC"
                     /locus_tag="PXA33_000186"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HDL5119568.1"
                     /translation="MTTFTHINQNGEANMVDVSMKQETVRIARAEAFVRMNAETLQMI
                     VSGNHHKGDVFATARIAGIQAAKRTWELIPLCHPLLLSKVEVQLEALPESNQVRIESL
                     CKLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMIIENVRLLSKSGGKSGDFKVD"
     gene            26183..26428
                     /gene="moaD"
                     /locus_tag="PXA33_000187"
     CDS             26183..26428
                     /gene="moaD"
                     /locus_tag="PXA33_000187"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005631889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HDL5119569.1"
                     /translation="MIKILFFAQVRELVATDRLDVEPTFTTAEALREHLSQQGKKWQL
                     ALEKGKLLVAINQTICPLETEIKDGDEVAFFPPVTGG"
     gene            26430..26885
                     /gene="moaE"
                     /locus_tag="PXA33_000188"
     CDS             26430..26885
                     /gene="moaE"
                     /locus_tag="PXA33_000188"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HDL5119570.1"
                     /translation="MFETLIEVQEAEFDQNRIYQWLSEQHSVGAATLFIGKVREMNLG
                     DNVSGLFLEHYPAMTKKALQEIVAQARTRWDLQRVAVIHRIGQLHTGDEIVLVGVSSA
                     HRGDAYHANEFIMDYLKTKAPFWKREQTQAGERWIEGRESDQQAAKKWQ"
     gene            26918..27400
                     /gene="ybeY"
                     /locus_tag="PXA33_000189"
     CDS             26918..27400
                     /gene="ybeY"
                     /locus_tag="PXA33_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HDL5119571.1"
                     /translation="MENLYIDLQIACENTENLPSEQQFYTWVQKALAVEAKTDDFPES
                     EITIRIVDEAESHELNLTYRGKDKPTNVLSFPFEAPEGIEMPLLGDLVICRQVMEKEA
                     EEQGISAESHWAHLAIHGTLHLLGYDHIEEDEAVEMESLETEIMQSLGYEDPYLSEKI
                     "
     gene            27478..29496
                     /locus_tag="PXA33_000190"
     CDS             27478..29496
                     /locus_tag="PXA33_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(Met) cytidine acetyltransferase"
                     /protein_id="HDL5119572.1"
                     /translation="MRTIAIITSPNNADFIQQGYFYIPENHQLKAIPFSNAKTILGQE
                     YPFAIYTMQAENGVNFNLDAFAILAGTIQENGTLFLLCPQWDNLENELDFDALRWNEN
                     QAIACPNFYQHFKQLVAKFGFEVRTNLPKLPATSGQIPSKIYQLTQEQQNILQNLPLD
                     PADIHLITAPRGRGKSTLAGRLAKALPAQNKVLITARSYSALPSFWRSAEQKIPFFAP
                     DNLIEQVKSKNISPNQWLFIDEAASLPLPILHTLCAYFEKVVLTTTTQNYEGTGRGFE
                     LKFPVMLDKPFKHWTLSQPLRWSENDPLEAFVTELLLLSEEKSPLFSKEGKGEIYSLR
                     SHYVPLLKQRSNAKRLWQKQLSKNISYSNKHLQEKIAFYHLLANAHYKTTPTDLRRLF
                     DGENQLLFSQQENEQLIGGIWAMQEGGLDEKLTQAIWLGERRPQGSLVAQYLCFQGNL
                     PQACKLHSIRISRIAVLPEKQNQGVGKRLISQFILQNSVEKRPLDFISVSFGLTTPLH
                     AFWQSCGFQLVQITPNKEASSGYQSAMMIYPISEQGKLFAQQATQAFARDFALQPFFT
                     EICEELQNFEQFRPLATHNLDERDWKNLTAFAHGKRTFSAAYVSLKRLYLSDKARFSE
                     LDIFERKGQKISKMEVENYRKLVLQNLNNKSFKIIYRFYYIFLCFCSC"
     gene            29561..30751
                     /locus_tag="PXA33_000191"
     CDS             29561..30751
                     /locus_tag="PXA33_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/tyrosine/aromatic aminotransferase"
                     /protein_id="HDL5119573.1"
                     /translation="MFSHIQAAPADSILGLGEAFKADSREGKINLGIGVYMTDEGKTP
                     IVKAVKEAEKRLLETENSKNYLTIDGVQAFNAATQALLFGEGAEVITSGRAKTAQSLG
                     GTGALRIAAEFIKRHTNAKNVWISTPTWPNHNGIFDAVGVNVKGYRYYNKETNGLDWE
                     NLIADLSQAEAGDVVLLHGCCHNPTGIDPTPAQWEQLAALSAEKGWLPLFDFAYQGFA
                     NGLEEDAYGLRAFVKNNRELLVASSFSKNFGLYNERVGAFTLIADNADDANRAFTQIK
                     SIIRVLYSNPSAHGASAVAVALADPDLKAQWIAELDEMRNRIKEMRSQLVQKLKEKGA
                     NKDFSFITEQNGMFSFSGLTPEQVDKLKDEFAIYAVRSGRINVAGITSKNIDALADAI
                     VKVL"
     gene            complement(30858..32159)
                     /gene="folC"
                     /locus_tag="PXA33_000192"
     CDS             complement(30858..32159)
                     /gene="folC"
                     /locus_tag="PXA33_000192"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HDL5119574.1"
                     /translation="MNTLSIPKATDSLETWLSYLEQAHSKPIDMGLDRIRKVATELDL
                     LKPAPYVITVAGTNGKGSTCRLLEVTLLKAGFRVGVYSSPHLIHYNERVRINGEVLPD
                     ADHVQAFAKINAKKTASLTYFEFSTLSAFELFKQNQLDIVILEVGLGGRLDATNIIDP
                     SLAVIISIDIDHVAFLGDNREDIGREKAGIFRENIPVVIGEPDCPHSIKQIADSLNCH
                     AFYRGKDWQFEIKNNLFHWYSEEKSFTDLPIPLIPIPNAGTALAVLMQLPFEIDEKII
                     RQALNEAQMTGRFQTLTDTDFTHFSQPKPAAKVIIDVGHNPHAARYLADKLRELKQPN
                     QKIYAVFSALEDKDLSGIVEPLANIIDEWHCAGLDCWRGQTGEAVLAKLVNVLPNSTA
                     CSYENVAQASRVLFETANENDIVLVFGSFHTVADFMLWLET"
     gene            complement(32169..33065)
                     /gene="accD"
                     /locus_tag="PXA33_000193"
     CDS             complement(32169..33065)
                     /gene="accD"
                     /locus_tag="PXA33_000193"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HDL5119575.1"
                     /translation="MSWLDRILGRDSSSSSNAKSKVPEGVWTKCTSCEQVLYSEELQR
                     NQQVCPKCDHHMRIDARTRLLNLLDKDSAVEIAADLEPEDVLKFKDLKKYKDRLTAAQ
                     KQTGEKDSFIVLAGTLHNMPVVTASFNFEFMGGSMGSVVGAKFVKAAEKALAEKIPFI
                     CFSASGGARMQEALFSLMQMAKTSAILAKMKEQGVPFISVLTDPTLGGVSASLAMLGD
                     VNIAEPKALIGFAGPRVIEQTVREKLPEGFQRAEFLLEHGAVDMIVHRKEMRDTLSRL
                     CAKLTNQPTPFKVAELVVENAE"
     gene            33374..34156
                     /gene="mazG"
                     /locus_tag="PXA33_000194"
                     /pseudo
     CDS             33374..34156
                     /gene="mazG"
                     /locus_tag="PXA33_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991517.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
BASE COUNT         9894 a         7445 c         6725 g        10098 t
ORIGIN      
        1 ttctctcctc ctttattaaa aaagaaaacg gtcagattgg taaaatctat acctaatatg
       61 accgcttgtg aatacaaaaa aattacttca attacttcaa ctgtttaaag gcttgcagta
      121 aatcagcttt aatatcttca acgtgttcta aaccaacgga aaaacgcagt aaattattcg
      181 taatgcctcg tgtaatacgt tctgcttccg gaatatccat atgggtttgt gttgcaggat
      241 aagtaataaa actttctgca ccgcctaaac tttccgcaaa ggtaatcaat ttaattgcct
      301 tgagaaattt cgctacccac tcttcttttt gtaaacggaa agataacata ccacttttgc
      361 ctgaatataa cacgctgtca atttcaggtt gctctgctaa gaatttagcc agttcggtgg
      421 cattagactg atggcgttcc attcgtaatg ccaaggtttt cataccacgc acggcaaggt
      481 aagaatcaaa tggcgacagc actgcaccgg cacagttttg gaaatagaat aaacgctcgg
      541 tcaaattctg cccgtttact ttggtagcat cagaatcttt cgcaacaatt aagccggcta
      601 acacatcatt atgccccgaa agatacttgg tcgcactgtg aatcacaata tcagctccgt
      661 gctcaatcgg acggaataac gctggggtta agaacgtatt atcaacgatc aacattaaat
      721 tgtgtttttt cgcaatttta gcaatggcag caacgtcgca ctcctccatc aatggatttg
      781 acggcgtttc cacgaaaatc gccttagtat tcggtgtaat agctgcttca atcgctgcta
      841 aatccgctgt attaacataa accggcttta atgtgccgtg atttttgtgg ctaaaatcaa
      901 acaagcggta agaaccacca taaacatcgc tggaaataat ccattcatca ggtgctttaa
      961 ataatgacat tactagctga atcgctgcca ttccagatgc catcgcaaaa ccggcatcac
     1021 cgccttctaa atccgcaatc ccctgctcta atacagctcg agttgggttt ttggtacggg
     1081 tgtaatccca gccggtagat tcgcctaagc catcacgccc gtaagcagtt gataagataa
     1141 tcggcattgc caccgcccct gtacgaggat cagttctgtt accgatttgc actaatgtgg
     1201 tttcaatatt attgaatttt gtcattgtta ttatccttca attacagatt atttagatcc
     1261 aatacgtctg tcatatcaaa caagccattt tgtttgccat ttaaccattt tgcagcacgc
     1321 actgcgccat tggcaaacgt cattcgacta gaggctttat gtgcgatttc gacacgttcg
     1381 ccttcatcag cgaaccatac actatgctcc cctacaacat caccggcacg aatagtagca
     1441 aagccgattt catcacgttt gcgttcgcct gtaataccgt tacgttcaaa tacgccatga
     1501 gttttcaagt cacgcccaag agttttggca atatgttctc ccattgataa agccgtacct
     1561 gatggtgcat caactttatg gcgatggtgt gcctcaatca cttcaatatc acaataatcg
     1621 cccattactt ttgctgcttt ttcgagcagt ttaaacacca gattcacccc aacgctgtag
     1681 tttgaggcaa aaacgatacc gattttttca ctcgctgctt tgattgcagc tttacctgca
     1741 tcatcaaaac cggttgtgcc aatcaccatt tgtttatgat gagccacaca aatatccaaa
     1801 tgttctagcg tgccttcagg gcgagtaaaa tcaattagta aatcaaaacc tgctacattt
     1861 tgggcgacat catcagaaat caccacacct aatgtaccaa ccccggctac ttcaccagca
     1921 tcactaccta ctaatgatga acctttacgc tcaaaagcag accctaattc tacgccttgt
     1981 gcattatgga cggcttgaat taattgacgc cccattcgcc caccggcacc gacgattcca
     2041 atttttaatg tcatatttgt tcttcctttt tatatttgtt ttttacaagc ggttacattt
     2101 ttaattaatt ttgcaaatag ctttctgatg gcaatttaaa aggtgggtga gaagtaaaat
     2161 caacaatttg aataaagttt tcacctttca cataatgtaa aatatactca gaagtataat
     2221 taccaaatcc ttgactggta tcatattctg gaatgccgat atgataatgc cataaagaat
     2281 atttttgtgc tttagccacc ttctctgccc aataaggatc attcgttggc acatcatcag
     2341 aacttttatt tcgaccagct agattttcca atccataaat ttcaacgtgt tgtttaaact
     2401 gcaggatttt gactagatct ttttcaggca aatattgcaa ttcggctgaa atttttgtac
     2461 caaataaaac tttaatgccc atttgcaaca cctttcaacc agtttctaaa ttcattaaca
     2521 ttcgttatat ctttaggcac ttcgtgaaaa gtatcattta accgctcttt cattcgctct
     2581 aagtcaaaat taaatgcggt tgcttgagag agttgctgtt caattttgcg aatattggac
     2641 ttcatattat ccaatatagc cattgtcctt gagccatcag taaaatactg ttgggtatcg
     2701 aaaatcacca ccttattttg atttaggtat tttttgaaat cgtttaaatc aatttcagcc
     2761 caaaagtttg gatttttttc gatagtttca atacaggcaa ttaataattg atttgccatt
     2821 gtaatacgaa aatttaacaa tgagatttgc tgctctgttg caggtttatc atcagctgcg
     2881 aaatcaaatg ccggataaat cactgcaagc gtgtcagcca tagataaggg taaatttttc
     2941 atcatctctc cttacggaag aagttgtcat tatctactgg attacttaaa aaaacaactt
     3001 attcatttta tgctattttt gccccatcac cgcaatatgt accgtaattt cctcacggtc
     3061 gtggtagagg tgtttagctt gcattttgaa ccaaaacccg tgtttcgtta gctcttcttc
     3121 caagaaattg aggcatagtt gcacttcttg ataacgtttt ttcatcggca gttttaggtt
     3181 gaaaatcgtt tctcgacacc aaccgttagt cagccatttt gcaatgagtt tcgagatacg
     3241 aatcggctgc tctaccatat cacaaaccag ccaatcgatt tgtttacgct taggcggttg
     3301 gaatttaaac ccatcttcag ggcaatgctc aattcttccg gtttcgtgta aacttgctgc
     3361 cattttgccg tgatcgactg catagacaaa aacaccacgt ttgactaatt gataagtcca
     3421 accacccgga caagccccaa gatcgacccc taccatcgta tcattaaagc gttttttctc
     3481 ctctgcttgc ggaataaaag ttaaaatcgc ctcttctaat ttaagcgttg agcgactagg
     3541 tgcctcagcc gggaatttta aacggggaat ccccataaaa aacggcgatt tattatggtt
     3601 gtacgcatag cccacatagc aagctcccgg acgagtaaat aaaatatgca agctcacact
     3661 accctgcata ttttccgatt tccccaacca accttgtttt ttcaatgccg accgtaatgg
     3721 cacagtaaat ttacgacaga aagttaataa ttctttcgct tcgttggtat ccggtgtttc
     3781 gacaaataca tcagaacttt ggcgaggatt aagttcggca tattgggcta aaatcggcga
     3841 aattcgatca ttttcagaca gattttgcaa caaatcccca acaaccaaca tttggcgaac
     3901 aaaaataagc tgttcaaact ttaacgcttg ggctaatcga tctgcttccc ctgcttgata
     3961 gcactcaaaa atcacatagc cactattttc tttaaggttg gcaaagccaa aaatgccctg
     4021 ttgtgcagct ttatccgtga tttcgcctgc tacctctttt tcaaaccctg ctcggcaata
     4081 taaggctaat ttattgaaca ttaggctttt ctcacactga tataaattaa cgcaatccaa
     4141 ccaagcataa agcagagacc accaataggc gtaagccaat gaatagtcgg cacatttaac
     4201 gccagtaagt acaggttgcc actaaaaagc agaatgccta acgcccaatt gcttccgatg
     4261 atgttaaagg ctttggctct acaaacgggt ggattttgcc caaaatttgc aatttggaat
     4321 aaacctaaag ctaaaatggc aagggtgtga aacatttgat atttaatccc cgtctcaatc
     4381 cagcctaagg ctttaggcga taattgagcc gataatgcgt ggctggctat cgccccaaat
     4441 gccacagtaa ataagccgct gaatgcggca attgctacaa atttgttttt cataagttga
     4501 agataggtat gatgttagat ggcttactat atcatattat cgaaaccaac aagcggtgaa
     4561 attttgcaaa aaaattacaa aaaatcaccg tttgtatgct taatggttca agataatatc
     4621 cacatcttcc gatttatcgt gaaccccaaa tctatctact aaggttttgc tggcctcatt
     4681 taatccgata atttctacct caacatcact ttttcgatat ttcaacacaa ctttatctaa
     4741 cgctgaaact gccgttacgt cccaaaagtg agcgtgggtt aaatcaatgt ggactttttt
     4801 cagttctgaa cgggtaaaat caaattgtgc gataaaatca tcagatgagg caaaaaatac
     4861 ttgcccgata acatcataac gcaattcctc tttctctata gcacttgttt taaccgccat
     4921 ataacgtcca attttattcg caaagaataa tgccgagagc aacacgccgg aaaatacacc
     4981 aagggctaag ttatgtgtat aaaccacaat tgcaacagtg actaacatca ctacattaaa
     5041 gccgattggg tgcgttttta gctcttttaa ggaatgccaa ttaaaggtgc cgatagagac
     5101 cataatcatc actgcaacta atgcagccat cggaatcact ctcaaaatat cgctcaaaaa
     5161 gaccacaaaa atcagtaata atattggcaa tgccctgccc tttacattca cggtttttat
     5221 cgctgtctgt ctctgtcatt tcatcaacaa tcgttgccgt cattaaagat tccaataacc
     5281 ctactgcggc caagctgaaa gaataaggca aaataatcca taaggtgtct aaattaagcg
     5341 gaatatcggg taataaaaac accggtaagg tatcgggtaa ttgccccata tccccaacgg
     5401 tacgaacatc aatgccttgc cataatgtga aaagtgttaa agcaataatg gtcactaatg
     5461 gtgagggaat gatttttcca atcaccggta cataaggaaa aagataaatg atagcaagcc
     5521 ctgctaccac cagtgcataa gtatgccaag ttgtattttg taactcaggt agttgtgcca
     5581 taaaaattaa aatggcaagt gcattaacaa acccaatcac tacggctcgt gccacaaacc
     5641 gcataagcga gccgagtttt aaccaaccgg caataatttg caacacgcct gttaataagg
     5701 tggccgctag catatattgt aaaccgtgat ccctcactaa gcctgctaat accagagcca
     5761 ttgcaccggt tgctgcagaa atcatcgccg gtctgccgcc cacaaaactg ataattactg
     5821 caatacaaaa tgaggcatat aaaccaattt gtggatcaac ccctgcaata attgaaaatg
     5881 ctatggcttc tggaattaac gccaaagcca ccactaaacc agagagaaca tcacctcgta
     5941 catttgaaaa ccattgttgt ttaattaccg taggcgaaaa ttgctgaaat aaagttgtca
     6001 ttattaccct taaataaaaa gaaaagccga gcaaagtcgg ctaaaaatga ggcgagagag
     6061 tagcaaatta gtgaaaagtc gtcaaataaa caagcggtct tctttcgctc tttttttgca
     6121 aaaaatagta gaaaaatgac cgcttggatt aattgctatt cactaaaaaa aggggcttta
     6181 aacaccgtca cttcaggtgc atctcccaca tatttttcca cttgaaccgt ggtagtgttc
     6241 ggtgcattgc cctgcccgag ttttgatgaa ccctcattac gggttagcac attcgggttg
     6301 ccgtttttgc atagtggagt atctgttttg cctaaatctt gcgggtcata ccaaccacct
     6361 tcgtataacg ctactgtgcc ttgaatgatg ccatcagtca ccactgctcc tgccaacacc
     6421 tgccctcgct tgttaaaaat tcgcacaata tcaccgcttg taatgcctct gtcagcggca
     6481 tcttttgtgt gaattagcat tggttctctg ccgtttacct cgtaatattg acgtaatgag
     6541 gagtaagcaa gctggctgtg taaacggtat ttaatatgcg gtgtaactaa tgccaacggc
     6601 tcggcttcgt tggttttgcc tgaatattcg tgatgttcaa accaagtcgg gtgtccttta
     6661 caatcttcat aattcatctt ggcaatggtt tcggagaaaa tctcaatttt gcctgatggc
     6721 gttgccaatt tgtgtttaat cggatcagta cgataatctt gatagcgaac gaaagtatta
     6781 ttttgatgtt ctgcctcaaa gctcaacact ttattttccg cccaaaatgc ctcgaaatcc
     6841 agcaatggcg tattagcttt ttcccccgcc tcaaaggcaa cttggtaaaa ttccttaagc
     6901 cattccattt cgcttttgcc ttcggtaaat gcttgttctt taccggctcg tttcgctaat
     6961 tcggcaaaaa tatcgtaatc attcttcgcc tcaaattgcg gttcaaccac ctgtttcatc
     7021 gggataatat gttttgctac ataatcaccg gcaacggtga gatcgtttcg ctcgtagctg
     7081 gtggttgcgg gcaatacaat atctgccatt cgagcggtcg gtgtccagta acattcattg
     7141 acgatcatcg tttccggctg ttgccacgct ttcactaaat agttggtatc cgggtggtga
     7201 gcaaacggat taccccccgc ccaatagact aatttgatgt tcgggtaatg taatgtttgc
     7261 ccgttgtaat cataaggctg attcggtttg agcaacgcat cggcaattcg agacaccggg
     7321 aaaaacggca tatcggggtt aggtttacct gcattgattg cccctaaacg aacaccggaa
     7381 ttaacattta gcccattggt agaataacga taatttaagc cgaaaccgcc gcccggcaag
     7441 ccaatttgcc cgagcatagc ggcaagagtc actaacgccc agtggctttg ctccccgtgt
     7501 tgcaggcgtt gcaagcccca gccggacatc aacattgtgc ggtgagtaac aaaatcattt
     7561 gctaaggttt taatcacctc ctccggaatg tcacaaattt ggctcgccca ctctgctgtt
     7621 ttaggtaaat tatctgcttt tcctaacaca taatcagcaa attgctcgaa accaacagta
     7681 tatttagtta agaagtcgtg atcgtgcttg ttttcagtaa taagggtgtg ggcgatccct
     7741 aaaatcagtg caacatccgt gccggtatta ataggcaacc actctgcctc taaaaattga
     7801 caggtctcgc ttttcaccgg gtcgatacaa ataatccgtt taccgcttgc ttttaatttg
     7861 gcgagataat caatcccctc cggttcggca gaggtccacg caatgcgaag tgtattcagt
     7921 ggattagacg accaaattac caataattct gtgctttcta caattgtgtg ccaactggtt
     7981 tgttgagcat acacctcaat attgcctaat acataaggca taatcacttg caccgcaccg
     8041 gtggaataat cgcctttagt attggtaaag ccgcctgtga cgtttaaata acgttgcaaa
     8101 agcgtgcgag aggcgtgcag tgaaccactg ctataccagc cgtaagaacc gccgaaaatg
     8161 gattcgctgc catattgctc tcttacccgt tggatttcgc cggaaaccag ctcaaaggct
     8221 ttgtcccaag agattctcac ccacttatct cgccctcgca aagtggtgtc tttattccct
     8281 tccaagtagc ctttccgcac catcggatat ttcacccgaa ccgggctata gagctgatcc
     8341 gccaccacat cttgcaattc gttttggtga tttttcgcta aactgccctc tgatttcacc
     8401 acttttccgt tttccaccgt tacatttaat gcaccccagt gggcggcggt ggtaatggta
     8461 tttttcggtt tatccaaaaa gacaaacttc cctttttcta atgctgtgtt catttaaatt
     8521 aatgcctttt ctgttacaaa attgagagga atttactgga ttaagccaaa agttgcaacc
     8581 acaagcggtt gtttttagcc aaaaatttgc aaaacaaaac ccacctaaaa aggtgggctt
     8641 caaggaagtt aaacggcaac ttgaacgatt acattgtccg cttttttcac ataactattg
     8701 atttggtcaa agttcatata gcggtaaata tcgtccttat ctttctgcaa gttattcata
     8761 tagtctaggt actcttgcgg tgtcggcaat ttacccaata acgcagaaac tgcggctaat
     8821 tctgctgagg ctaaatacac atttgcccct tgccctaaac ggttcgggaa gtttcgggta
     8881 gaggtagaaa ccacattagc attatttgcc actcgggctt ggttacccat acagagagaa
     8941 cagcccggca tctctaccct tgctccgctt ttgccgtaaa tgctgtaata gccctcttcg
     9001 gtcagcaagc ctgcgtccat tttagtcggc ggagcaatcc ataatttagt cggcaattcg
     9061 ccctgtgctt ggcttaacag tttacctgcc gcacggaaat gcccgatatt agtcatacaa
     9121 gaaccaataa agacttcatc aattttatcg ccctgtactt gagaaagcag gcgagcatcg
     9181 tcagggtcat tcggtacgca cacaatcggc tctttgattt catctaagtt gatctcaatc
     9241 actgcggcat actccgcatt ttcgtctgcc tctaaaagct ccggattatc caaccacgct
     9301 tgcatattgg cgatacggcg ttctagcgtg cgtttatcgt catagccttc cgcaatcatc
     9361 cattttaaaa gcgtaatatt agaattcaga tactcgataa tcggctcttt gttgagcttg
     9421 atggtacagg ctgcagcaga gcgttcagca gaagcatcag aaagctcgaa ggcttgttcg
     9481 attttcagat cttccaaccc ttcaatttct aaaattcgac cggagaaaat atttttctta
     9541 ccttttttct ccaccgtcag caagccttgc tgaatggcat aatacggaat agcgtgaacc
     9601 agatcccgta aggtaatgcc cggttgcatt gtgccggaaa aacgcaccaa gaccgattcc
     9661 ggcatatcta acggcatcac ccctgttgcc gcagcaaagg caactaagcc cgaaccggca
     9721 gggaatgaaa tcccgattgg gaaacgggtg tgggaatcgc cgcctgtacc gacagtatcc
     9781 ggcaataaca tacggtttag ccaagagtga atcacgccat cacccgggcg aagtgacaca
     9841 ccgccacggt tcataataaa atcaggcaaa gtgtggtggg taattacgtc caccggtttc
     9901 gggtaagcgg cagtatggca gaacgattgc atcacaagat ctgccgagaa gcctaaacag
     9961 gctaaatctt tcagctcatc tcttgtcatt ggtcctgtgg tatcttgtga accgacagac
    10021 gtcattcgag gctcgcaata ttgccccggt cgaacgcctt ctacgccaca agctcgccct
    10081 accatttttt gagcaagggt gtagcctttg ttactctctg aaaccgactg cggtttaata
    10141 aatacatcac tttcaggcaa gccaagtgct actctggctt tatgggtcaa accacgtccg
    10201 ataatcagcg gaattcgccc gcctgcacgc acttcatcaa gcaatacttc ggtttttagc
    10261 tcaaattcgg ctaagacttc atcggagttg tgtttgcaaa ttttgcctgc ataagggtaa
    10321 atgtcgatta catcgcccat attcaggttg gttacatcca cttcgatcgg taaagaaccg
    10381 gcatcttcta atgtgttaaa gaaaatcggg gcaattttac cgcctaacac caccccaccg
    10441 gcacgtttgt ttggaatgta aggaatatct tcccccataa accaaagcac ggagttggtt
    10501 gcggatttac gagaagagcc ggtgccgact acatcaccga cataggctaa cgggaaacct
    10561 ttttctttta atgcttccag ttgcttaatc gggccggtat gaccgttttc atcggggaaa
    10621 ataccgtcac gttcattttt cagcattgct aatgcgtgaa gtggaatatc agggcgagac
    10681 caagcgtctt gagccgggga aagatcatcg gtattggttt cgcctgtgac tttaaatacc
    10741 gttaccgtta atttttcggc aagtttcggg cgagaggtaa accaatcggc attcgcccaa
    10801 gattccaata cttgttgtgc aaacggatta ccctgatctg cccgttcttt tacatcgtgg
    10861 aaattatcaa acatcaataa tgttttagat aaggcttcta ccgcaatcgg tgcaagctca
    10921 gggttatcca acgcagacaa taaggtttca atgttataac caccttgcat tgtgccgagt
    10981 aatttcactg cgtgagcagg cgaaatgagc ggcgatgtgg caaccccttt ggtaatatcg
    11041 gctaaaaagg cggctttaac ataagcggct tcatcaacac ctgccggaat acgattggta
    11101 aaaagatcca ataaaaagac cgcttgttca gccggtgggt ttttcaataa ttcaaccagt
    11161 tgagccgttt gttccgcaga aagtggctga ggcacaacac ccagtgccgc acgttcatca
    11221 acctgttgtt gataattttc tataaaattt tccatcatca ttccctctcc aactttacat
    11281 attggcaata atgtcttgtg caaattctga ggttgagcgt agagttaccc cctccagcat
    11341 ttccgcaaaa tcaaaggtga cagttttatt ggcaatggtt ttagacaccg cttttaccac
    11401 taaatcagcc gcttcaaccc aacctaaatg gcgtaacatc atttccccac ttaaaattaa
    11461 tgacccagga ttgcctttat tttgccctgc aattttaggg gctgtgccgt gtgtggcttc
    11521 aaaaatagcc gcttcaaacc caatattcgc tcccggtgaa ataccgattc cgcctacttg
    11581 tgcggctaaa gcatcagaaa tatagtcgcc gtttaggttc agtgtagcaa tcacatcata
    11641 ttcggttggg tgtaacaaga tttcttgtaa aaaggcatcg gcaatgctgt cttttataat
    11701 gatttcctca ccggttttcg gattagtcaa gctcatccaa gggcctttat caattaattt
    11761 tgccccaaat tcttccgcaa cttggtagcc ccactcttta aatgctcctt cggtaaattt
    11821 cataatgtta cctttatgca caagcgtgag cgatttacgg ttgttatcaa ttacatattg
    11881 caatgcagca cgcaccaagc gttgtgtgcc ttgcttagaa accggcttaa ttccgatacc
    11941 gcaatcttcg gtaaagcgga tttttttcac gcccatctct tgttgtaaaa attcgatcac
    12001 tttattggct tcaggcgaac cggcaaccca ttctacaccg gcataaatat cttccgagtt
    12061 ttcacggaaa atcaccatat ccaccagctc agggtgttta accgggcttg gcgtaccgtc
    12121 ataataacga attgggcgta agcagttgta taaatctaat ccttgacgca tcgccacatt
    12181 taaagaacga ataccgccac ccaccggtgt cattaaagga cctttaatcg ccacgtgata
    12241 ttggcggata agctctaagg tttcatccgg caaccaagtg ttctcaccat ataatttatt
    12301 cgccttttcg cctgcgtaaa tttccatcca agcaattttt cgcttgcctt gataggcttt
    12361 ttctaccgcc gcatctagca ctgctttcat tgcaggagta acatcaacac cgatgccgtc
    12421 accttcaata aatggaataa tcggattatc cggaacttgt aaagaaccgt cttggttcag
    12481 ttgaatagtt tcgccttgtg gaatttggac ttgtgtttgc attgatttta cccccgtaat
    12541 gacgaaaaag tagtgacgaa aaatgaatgt tgacaatcag ccaacaatgg attaactata
    12601 aacagtaaat ttctaaatgt aaataaagca aaatgggctg agagagaatt tgtgatctaa
    12661 cgcacattta tgtaaataaa atgtaaaaaa tcgttgtgat tcttttggaa atcatctagt
    12721 ttatcttata aatctcaaat aaataggagt aagctatgtc aaaagcaaca ttaacactcg
    12781 aaacaggtga aaaacttgaa ctcaatatgc aaaaaggaac cttagggtat caaacgattg
    12841 atattcagcc tttcttaaag cataagttat ttgcttatga tcccggattg gtttcgaccg
    12901 ctgtatgccg atccaatatc acttatgttg atggcgatga aggggtttta ctctatcgag
    12961 gctacccgat tgaccaatta gccaaacatt ctgattactt agaagtcggt tatatgctgt
    13021 tattcggcga acgccctacc aaagaagaat acaaagactt cgttcaactc atcaaaaaac
    13081 acactcttgt acacgaacaa ctaaccaaat tcttctccgg tttccgccga gattcgcacc
    13141 caatggcggt gatgtgtggg gtaagtggag ctttggctgc cttctaccac gacgccattg
    13201 atgtaaacaa ccaagcccac cgtgaattaa ccgccattcg tttgttagcc aaaatcccaa
    13261 cccttgcggc gatgtgttat aaatactcta tcggtcagcc gtttatgttc ccacaaaacc
    13321 acttatctta cgccggcaac ttcttatata tgatgtttgc cactccttgt gaaccgtacg
    13381 aagtgaaccc tgtgttagag cgtgcgttag accgaatttt cattttacat gcagaccacg
    13441 aacaaaacgc ttcgacctca accgtgcgaa ccgccgcttc atcaggtgca aacccatttg
    13501 cttgcattgc tgccggtatt gcctctctct ggggacctgc acacggagga gcaaatgaag
    13561 cctgtatcaa tatgttgaaa gaaatcggca ctgtggatcg cattcctgaa tttattgctc
    13621 gagccaaaga taaaaatgac ccgttccgct taatgggctt tgggcatcgt gtctataaaa
    13681 actacgaccc tcgtgcgaaa gtaatgcgtg aaacctgcca cgaagtgtta aaagaactga
    13741 acattgataa cccactattt gaagtggcat tggaattaga acgtattgcg ttaagcgacc
    13801 cttatttcat tgagcataaa ctttatccaa acgtagattt ctactccgga atcgtgctaa
    13861 aagccatcgg tattccaacc tcaatgttta ccgtaatgtt tgccctagcc agaaccgttg
    13921 gctggattgc tcactggaaa gagatgtatc tccaaggcaa tatcaaaatc gttcgtccac
    13981 gtcaaatcta caccggacat actaaacgtg attttgagcc aatggataaa tcataagatt
    14041 ggcaaaaaat cgaaaaattt taaccgcttg tttccacaag cggttatatt ttgcataaaa
    14101 tttgcaaatc aggctaattt atttagcagt tgtgttctaa catcttgacc attttcatcg
    14161 ctttttacag ccataataaa taagcctttt ccttgactta atttctccca aagattgcca
    14221 attaaggctt tttctttaga atcttcatta ctttttaatt gttccccttt atattccaca
    14281 attaaaattc gaccatcaat aagctctaca ataaaatcag ggtaaaaatt ctgatgagct
    14341 aatggtaaag aaaaccctct ttttacagga tttcttaacc aatcatcaga aaaactcagt
    14401 tgctgctgat taaaatggta agaaacccgt ttatcttcca catcaatttc aaaactttgg
    14461 ctattttctt gtaatctaaa tccatcaagt ttggctggat aatccaataa attccaactt
    14521 tggtaatcta atagcacttg tgaatcagct aagtcgaatt cgccttgaat attcatacac
    14581 agttgaggta ttactttaaa tatttcccct ttttcggcag gagaagataa tatttcaact
    14641 ctattctggt gaatttgtaa ctgttgctca atttgctctt ttttcttacc tgttgcagtt
    14701 cgaatcaacg ctttttctaa cttttcactt aaattacctt ttacttgcac taaaatgtga
    14761 ccttcttgct cggtaatttt aagttgagaa cgttttgctt cgcttaaatc agacatttca
    14821 gggagttgcg ttaatgctaa aactgttgtc ggtgtataga ataaatctcc ttcgtttcca
    14881 atccaatttt cttgttgtgg gatacgaagc atttcagcta cttctaatgc ttcaaacccc
    14941 attccaatca atttatcttg taaattacga gccgtttcgc cgaacgactt agaggaaagc
    15001 ggagcataag cacgatttaa atcttcaatt tgacggtgtt tagcataagg cattcgcaat
    15061 actcgcccta atagctgctc cgcttccttg ctagaggaaa cattttgtac tgagcaaaaa
    15121 atataagcaa aagggcaatc ccacccttct tttaatgcct caaccgtgat gatgtaattg
    15181 atcttgcatt gtggattaaa taaatcgata ttatctaatt cgtgttgatt acctatcaca
    15241 atggcaatct cttcttcatc aatttttaac tcatcaatta aatactgttt taagacttct
    15301 actgttactt tgccgttttt cggctcagct tgaaacaata aatcggacga acgtactcgc
    15361 tctcatactg tgctttttgt gctaatgctc ctctattgat tacagcccca tctattgctt
    15421 gttgccagtt gtgatgttcg gtcagcacaa taggcaattt gatcatctct tctgctttta
    15481 gatggcttgc cgaaacgtga tacaacacat tacttcctgc ttttctatct gtgtttggcg
    15541 tagcagtaaa ttctaaaata gctgatgggg aaagttgaga tagcacatca aaagagagtg
    15601 aagtccgagc attatgtgct tcatcaacaa tgaccaaagg ctgataagct cgcaacaaat
    15661 ttgcaaaaga acacttaatt ttgcccgttt gtttggaggt taaaccgttc tcttgtagat
    15721 cagtagtgga aatcttttct aaatttgcct gaatatgggg tagtaaacgc tcaaaatgag
    15781 ctgttaattt ttcattaaaa gcatagattt ttcgcccatc ttgattttct acccgaaaat
    15841 tttgaatagt agaaacgatt acaatcgcct tattaccaaa atcttgtggg cgaagtaaat
    15901 caaaatcttc agactcaata accattactt ctcggttaaa agttttatca agtactgcac
    15961 ggtaagggtg ttgcggattt tttaatgctt ctgtagtttg ggttttaatt gtacgagaag
    16021 gcactggcca caaggtaacc gggaaatcgc aatccaaata ctgctttgca atacgagcaa
    16081 tactatgcga ggctaacaac gttttaccgc caccagttgg aattcgtaca caaacataag
    16141 gcacatcacg caaattactg tgttcacttg ggatcttgta actatattca ggttgaattt
    16201 ggctaaatgc ggtttttgca tcagattgca aacaagccga aaaatagtgg ctgacacggt
    16261 ttaatgcctc attttggtaa ttttttaatt tcattttttt ctcctactat taacgagaca
    16321 ttacatcata tggaatctgt ttaaatgtaa ttttcttttc agctaactgc acttctccta
    16381 aacggcacgc ttcgccatat accacaatat ccattccctg ttgaataaat tcagcaaagt
    16441 atggtaactc cgccattaat tttcgtgtca gaacattacc actctgcgga cgtttatcgc
    16501 ctaaaattcc gttatataac aaatagtagg ctttgccgtt gaaagtgccg acaagcggtg
    16561 atttttcgct gtttttttgc aaagggaaat gattttctaa ataccaaata tgggcggcta
    16621 aatcgtcaaa acgaatgttc ggattaagtg aaccgaatgc gtcaaatact tcctcgccta
    16681 actcacaaaa gcgaaacgat ccaccacctt gccaatttac tgcttttgaa ataccgcctt
    16741 gctcaccttc aattactttt ttcaaccgtg ggacaacatg ggttttagca tgttcgccaa
    16801 tctcaatacc gatataacga cggttcattt tatgagcaac tgctgcagtt gtacctgagc
    16861 caagaaagct atcgaggacg agatcatatg gttcagtcga tgcttctaat acaaattaga
    16921 ttaatttttc aggtttagga taatcaaatg aatcttctgc aatatcctcc ccaaataatt
    16981 ctagatgttc actagcagag tcttcatttg ttgccatatc ataatgagct ctgctaatta
    17041 agttatgaat ttttttcttt tccgcacttc gctcaaatac tctccttggt cgaaagtcta
    17101 aactttttat aatatagtaa tcaccatttt ttaattgctc atcaatactt ttttggctat
    17161 accgccattc accttcaatt tctaccgtat ttttattcca ttgatcttca ataattaatt
    17221 ccgaaatcaa cgtaattgaa ctcgtttttt tagaaaaaat ggttggctta tataatccat
    17281 cactatgttt tttaaatttt atagaacgag gagggaaaac taaaatttct cttgaatttc
    17341 cagcattgta taatggatag gtttcatcac ctgaggtctc aaggtaagtt aagtcattta
    17401 attcatttaa ttcatttttg ttttttgcat agcaaaataa attatctgta actttaccaa
    17461 ctttgcctaa aaaagatggt ttccttttct tttcccaaat taagttagaa atgaaattct
    17521 tccgcccaaa aatttcatca agcataattt tcaaatagtg actttcgtta tcatcaagag
    17581 tgatccaaat tgaaccatct tcggcaagta actctcgcag aatttcaagg cgtggataca
    17641 tcattgaaag ccaaatggaa tgctctaaat tatcatcgta gtgggtaaag gccgattttg
    17701 tgttataggg aggatcaata aaaatacatt tgacttgccc cgcataaaag ggcaaaagag
    17761 attttaaggc tgataaatta tcaccctgaa taattaaatt ttgcttgact ttagataagg
    17821 tttcagcaga agctgagctg tcggctgtcg gctgtcggct gtcggctgtc ggctgtcggc
    17881 tgtcggctgt cggctgtcgg ctgtcggctg tcggctgtcg ggcaatatta tgcgagaagt
    17941 ccggcaactc acgcaatagg caatatgggg tttgttttgc ttttttgacg gcttcttctt
    18001 tgccgtacca agtaagggtt gtcataaaat gcacctgaaa agtaaaagtg gattattgta
    18061 acaagcggtc aaaaaaatag aaagttttac aaaaaattaa gcaaatttaa ccgcttgcaa
    18121 catagaaaag gcacagtttt atactgtgcc tttggttata tgaaattagc cttgtgtgta
    18181 tttaggctct ttaaatactg ttacttctgg tgcttgtccg gtgaattttt caacctgtac
    18241 cgtagcggta tttggtgagt taccctgtgc taattttgat gtgccttcat cacgggttag
    18301 cacgttaggg ttgccgtttt tacatagcgg ttgctcgctg gtgccgagat cgagcggatc
    18361 gtaccaagct ccttcgtgca aggctaatgt acctttgata atgccatctg tcaccaccgc
    18421 accggctaat acttgaccac gtttgttaaa aatacgtaca atatcaccgc ttgtaatgcc
    18481 acgagcggcg gcatcttcag tatggatcaa caccggctca cggtcattaa ccgcatattg
    18541 tttgcgtaat gatgagtaag ctaactggct gtgtaaacgc catttagagt gtggagtcac
    18601 caatgccaac ggttcttctg cggtggtttt acccgagtat tcttcgtgtt ccatccaagt
    18661 tgggtgacct ttacagtcat cataattcat tttggcaatc acctcagaga aaatctcaat
    18721 tttgccggac ggtgtgccta atggatttag gagtgggtca ttacgaaatt cttcataacg
    18781 aacccatttt ttcgctttgt ctgatgcttt aaaagaaagt ggtttattct cttgccagaa
    18841 tttctcgaat ttcggcataa tcacacgatt tttgcgtgct gcctcaaatg cagtttgata
    18901 gaaactttgt agccaatcca tttcagattt gccttcggta aattccgctt ctttgcctgc
    18961 acgtttggct agttcggcaa aaatatcgta gtcgtttttt gcctcaaatt gcggttcgac
    19021 cacctgtttc atcgggaaaa tattcatcat tgaataatcg cccgacatag ttaagtcatt
    19081 acgctcatag ctggtggttg ccggcagcac gatgtcagac atacgagccg ttggtgtcca
    19141 atggcattca ttgacgataa tagtatccgg tttttgccac gctttcacca aagtgttggt
    19201 gtcttggtgg tgggtaaacg gattaccccc tgcccaatag acaattttga tatcaggata
    19261 agtaatttgt gtgccattgt aatcaatggt tttgcccggg ttgagcagtg catcggaaat
    19321 acgagccacc gggaatgaag attttgatgt ttcatctaac caggttttcg ctccggcttc
    19381 tactgccgga tttgctgtga tagagcctaa aatgccccct gtggcactcg gcacgccgcc
    19441 gtttgagtaa tggtagctta aaccgaaacc accacccggt aagccgattt gacctaacat
    19501 tgcagctagg gtgactaaca tccaatgccc ttgttcaccg tgtcgttggc gttgaatccc
    19561 ccagcctgcc attaacattg tgcgtttaga ttggaaatca gtcgctaatt ttttaatggt
    19621 ttcttccggc acaccacaaa tttgacttgc ccattttgca tctttcggtt gattgtctgt
    19681 tttaccgagc agatattctt caaacttctc aaaaccggtg gtgtattttt taaggaatgc
    19741 ttgatcgtgt ttgccttcag cgactaaggt atgagccata ccgagcatta acgcaacatc
    19801 agtgccggtg tttaccggta cccattctgc acctaaaaat tcgcaagatt cactttttac
    19861 cgggtcaata cagataatgc gtttcccgct ctctttgagt tttttgaagt attcaatacc
    19921 ttgttgatcg gtagaagtcc acgcaatgcg tagcgtattt aacgggttag ctgaccaaag
    19981 caccacaata tctgaacttt ctaaaatcac ttcccaacta gtttgctgtt cgtacacctc
    20041 gattgtaccc aagacgtgtg gcataatcac ttgagcagca ccggtagaat agtcgccttt
    20101 tacaccaaca aatccgccgg taacatttaa atagcgttgt aacagtgtac gggaagcgtg
    20161 taatgcccca gaactatacc aaccgtaaga accaccaaaa atagcattcg ccccgtgagc
    20221 ctcacgaaca cgtttcattt cattcgcaac taattcaaat gccttatccc acgaaactcg
    20281 cacccattca tcacgcccac gtagagtagt atctttatta ccttctaagt agcctttacg
    20341 caccattggg tattttactc gtgtttcacc gtgtacttgg tctgctacca cgtgttgcaa
    20401 ttcatttggc acagaaatct caaaaacaga atcagatttc accactttgc cattttctac
    20461 cactacacct aaaggccccc agtgggcggc agttactact gttttcatct cggctgcttt
    20521 ggcactggtg gaagctaatg cccccactac accggtagta ccactggcta aacctgcacc
    20581 ggcagctaag cttaatgatg aattttttag aaaaccgcga cgacttacgt caatattgtc
    20641 ttgttttttc ataataaacc tcgtttgaag ttaagttaaa aatagacacc tttctaaagt
    20701 cgagtggata aaccatttct cgtgtatcca ttggtaaaag caccgacggt aatttttgca
    20761 aaatgttagg aaaaaatgac cgcttgtagt aggtgcttta cggcttgcta ccatatctct
    20821 gctctcagca gacacttagg ctcacaagct cggtatcctc taagttgcaa ttaatgggaa
    20881 ctgcctgcta catcttttgc attacgttgg agatagatag tcaccgcacg cacatcatca
    20941 gccgacatag atgttctgtc tttcattgaa tttaccaccc caatccattg atttgcggtg
    21001 taatgttctg ccccaatcgg tgcatggcaa ccgctacagt gtgtttggtt taagttatcg
    21061 ccaaaatgat ttagtgcagt aatattggca gtgactgcat ttttaggagc agtaatttca
    21121 aaattgattt ctttccaatt tgagttggtg acattatcaa acactgtttt tgtgacattc
    21181 actttgcttt gagcgtcatc aggtagaaga gcaaccataa tacgcttgcc taaatccata
    21241 tacaccacag attccgcacc ttcttgttgc cagccattaa ccgttgcttt aacctgctcg
    21301 ccttctgctg tccattgatg taattctgca taaggtaaca agcgaatttc tccaccttga
    21361 gcgagctgca ctggagtcat tgcgagcgta taaagggttt tatcatttgt gctaaattcc
    21421 gcaccgtgtt tagctaattc gcccattgct gcggcattat ctaccggcac atcaggcata
    21481 aaatgggcaa ttcctttatg gcaatcaata caggtttgat tagtttcttg ggctagtttg
    21541 tgcattttct gtgccgcttc tgtttgttca gaaagtacca tcgcatcaaa actatgacag
    21601 cttttacagg tcatagaatc gttttctttc atttctgccc aaacacgttg tgccatttct
    21661 agacgatggg cttcataggc ttcttgatca ggtaatttat tggttacaac ggtgtgccaa
    21721 acatctttta atgcgatgat tttagctttg acataatgca agccgtcttg tggtagatgg
    21781 caatccgaac actcggctcg aattccctta cggttggaaa aatggacact gccttcccac
    21841 tctgcctgtg ggtgagtcat agaatggcag ctcacacaaa attctgttga gctggtggct
    21901 ttcattgcat attgtgtgcc acctaatacc gcagcaccta atgccataat accgacaaat
    21961 acagcaattt ttttcatctt agccataaat taccccactt aatacactaa aaattaaaga
    22021 cagaggcatt gtattaactc ccaagaggta taaaaataag taaaaatacc tattacctcg
    22081 aaagagtgag aataatttga tctaaggcaa aaatatgtag tgaaatttta ggctttttca
    22141 gagaaccacg gcggtaagat aaattgtgca ttggcaaaca actccggtgc aatcacacgg
    22201 ctctgccctt gttggatttg gtaaaccaac tgtggcaagc tgatagataa gtcgtgggtt
    22261 tctgcgtaag ttaatccaat ttggctatcg gtattaaaat agtaagtgcc gtttacccca
    22321 taatgtggct gtaagcggat cgcattggag acggctttaa aatgccgtgg tgaaacactc
    22381 tgtttccacg catttgccag aatattcgct tggtcataag caatactggc ttgggaataa
    22441 gtgggcgatt tgccgaaaaa ccgttcgtaa tgttggctaa actgtctgcc taaataattt
    22501 tcgcttaacc cactattggt actccaaaga acgccttcgc aaagtttctc gtgtggcaaa
    22561 aacgccgaag gtgcataaat actgtaaata atggcatttg ttgggttgcg aatgaacgtt
    22621 tgataaaact gtacaatatc ttctgcaaaa taagaggcaa acacaataat ggaaggtgcg
    22681 agattatgca cctgtgccat taatgtggca taattatctt gctcttgggt gagttccaga
    22741 taatccacat tccagccttg ctcccgcaaa cttagggtta aactttcaat accgatatca
    22801 actttttgcc aactcaccgt taccaccatc accgttttat tctgaagcca aggggcataa
    22861 tggtaacggt aatattgcag aaagtgagat atacctaagc cataggttac atcactggcg
    22921 caaacctgaa acatattttc aattcgttga ttcggtaaat tttgggcaag tttatttgag
    22981 ccactatggg ttgcaatatg caaataagga atgcctttag ttgcaattaa ttcgtgtact
    23041 tccggcaagt agcaggcata agcggtggaa atcgcatcaa cttcttgatc aaataaatga
    23101 tggtatgcct gaagaatact tgctttatct ttaacataaa acccagccgt tgcaacctgt
    23161 acttctctgc cgtgaatacc accttgttga ttgatggtct caaccgcaag ttgagtgccg
    23221 ttaagtagct ctcgggtatc gatttgccca ataccctgtt caacataagg cacgccaata
    23281 ataatcggac gactatgatg gtggtttatc gtaaaaatcg gtggttcgtg tcgagttgaa
    23341 gccgttacaa gcggttgttt tttcgcgata atttgcaatg ccagttgttc aatgttaaag
    23401 ctggcaagaa tggattgggt tagttttccc ggtgtgggta aacagattaa atgttcggta
    23461 atggcaaaaa cagctaataa ggttcggctt ttggtatgtg ttttcgcaaa aatattttcg
    23521 atatgtttat caacagtccg tttgctgata caaagctcat tggcgatatc ctcattgctc
    23581 aaaccactgc taattagagt gagaagctca atctcccttt tactcagtcc ataaggcaaa
    23641 tcggtatgct ctgcttgagt caatacaaac gattggtggc gttctaggct gatcttaagc
    23701 cattgatggt ttgaactata gtaaaactgc aagctatttt tatctgaata gagcaatgcc
    23761 tgtgcatagt ggcaaatttg cggtaattca gcaaaatagt ctgcccctgc tttgtcttcc
    23821 cattctgata tttcgccttg ttcatttact aaacaagacc atcggctacg tttatccatt
    23881 ctcacctcga actaaaaacg ttagctaaaa tagcaaaatt atctcggcat taatatgata
    23941 taaatcaaac taagcaacgg ttgccagatt tttataggta gagcatacaa tttaaattat
    24001 acctcttgcc taattaggtt tctcaccata aaatgaaaac caatttattg aagactccga
    24061 gcattatttc ctaactctaa aaattaaaga caaatttttg atatggaatt tatgccagta
    24121 acagcataaa gctgttcaaa ggataactta gaaatgggtt gtcctctcgt gtttagaaag
    24181 gtgtcaaatg cagtctatta tcccaattaa aaacgtgggt gatgatgtcg tttcaggttt
    24241 agttgatcgc tttcagcgtc aatatactta tttgcgacta tccattactg atgtttgtaa
    24301 cttccgttgt aattattgct tgcctgatgg ctataagccg ccttcacata agcaaacttt
    24361 tcttagcgtt gatgaaatcc agcgggtggc taaagccttt gccaatttag ggacggaaaa
    24421 aatccgtatc accggtggtg agccaacttt acgcaaagat ttcctcgaaa tcgctcacac
    24481 tatttcacaa acacccggta tccgtaaagt tgccctaacc accaacggct atcgcctaga
    24541 gcgtgatgtt gaactgtggc aacaagccgg tattaccgat attaatgtga gtgttgatag
    24601 ccttgaccca cgccaatttc aactgattac cggcgagaac aagttgcaat ctattttaaa
    24661 gggtattgat aaagcctttg aaattggcta cccaaaaatt aaagtcaatg cggtgctaat
    24721 gaagcaatat accgcccctg aattggataa attcctcact tggatcaaag acaagccgat
    24781 tcaaatgcgg tttattgagc tgatggaaac cggtgaaatg gatagtttct tccaagctca
    24841 acatctttct ggtcaatcgg tgatgacccg cttattacaa gaaggctggc aattacaacc
    24901 caaagcgatg tctgacggcc cggcaaaagt gctttctcac cctgatttta aaggcgaaat
    24961 cggcttaatt atgccctacg agaaaaattt ctgtgcctct tgcaaccgct tgcgtgtttc
    25021 cgcacttggt aaactgcatc tttgcttatt tggtgaagaa gggattgata ttcgtgattt
    25081 actggcaagc gatgagcaac agttacagct tgaaacccgc ctaaaatctt ccttacaagg
    25141 caagcgagaa caccactatt tgcatattgg ggatagtggg gttaggaata atttggcgag
    25201 tatcggtggt taaacaacca ccgagcgtgt tttttctgtt acttttcttt gcttcgccaa
    25261 agaaaagtaa ccaaaagaaa ggcgacccca gattttcctt aatccttcgc ttgttgctaa
    25321 cttttgggac gaacttttca aactcgccaa ctgcgttggc tcaaacacga aaagttgtcc
    25381 tcaaaagttt acgcaatcgc tacggcggaa aataggggca agcggtacat tatttaagat
    25441 ttttgcaagt ttttatttaa aaataaccgc ttgtaatgtt ccagcttctc ccttctgaac
    25501 cgcctgagcg actttgaatt tataggaaaa atttagtgtt tgagccaacg aagttggcga
    25561 gttttaaatt tttccgttaa acaaattcaa ataaaatgaa gatagcggtg aagtagggtg
    25621 tgctttcttt ggttactttc tttgcacaag caaagaaagt aacaagaaga cgctcaatat
    25681 taattttaca atcaaattta aagagacaaa caaatgacaa ccttcaccca catcaaccaa
    25741 aacggcgaag ccaatatggt tgatgtttca atgaaacaag aaaccgtacg cattgcccga
    25801 gcggaggcat ttgtgcgaat gaatgccgaa acattacaaa tgatcgtcag tggtaatcac
    25861 cacaaaggcg atgtattcgc aaccgctcga attgccggta ttcaggcggc gaagagaact
    25921 tgggagctga ttccactctg ccacccgtta ttgctttcta aagtggaagt tcaactggag
    25981 gcgttgccgg aaagcaacca agtgcgaatt gaatcactct gtaaactaac cggcaaaacc
    26041 ggcgtggaaa tggaggcatt aaccgcagct agtgttgctg ctctgactat ttacgatatg
    26101 tgtaaagccg tgcaaaaaga tatgattatt gaaaatgtaa gattacttag taaaagtggc
    26161 ggaaaatcag gagatttcaa ggttgattaa gatacttttt ttcgcccaag tgcgtgaact
    26221 tgtggcaacg gatagactag atgttgagcc tacttttacc actgccgaag cgttgcgtga
    26281 acacttaagc caacagggta aaaaatggca acttgccctt gaaaaaggca aactgctggt
    26341 tgcaattaat caaacgatct gcccgcttga aacggagatc aaagacggcg atgaagtggc
    26401 attcttccca ccggtaaccg ggggctaaaa tgtttgaaac cctgattgaa gtacaagaag
    26461 ctgaatttga ccaaaaccgc atttaccaat ggctcagtga acaacatagc gtgggggcag
    26521 ccactctatt catcggtaaa gtacgtgaaa tgaatttggg cgataatgtt tccggtttgt
    26581 ttcttgaaca ctatccggca atgactaaaa aagcattgca agaaattgtg gctcaagctc
    26641 gcactcgttg ggatttacaa cgcgttgcgg tgattcaccg cattgggcaa ctgcataccg
    26701 gtgatgaaat cgtattagtc ggtgtgagtt ctgctcatcg aggtgatgcc taccacgcca
    26761 acgagtttat tatggattat ctcaaaacca aagccccatt ttggaaacga gaacaaaccc
    26821 aagccggtga acgttggatt gaagggcgtg agagtgacca acaagccgcc aaaaaatggc
    26881 aatagaaaaa atggcaataa tttgcaaaag gtaacgaatg gaaaatttat atattgattt
    26941 gcagatcgcc tgcgaaaata ccgaaaactt accaagtgaa caacaatttt atacttgggt
    27001 acaaaaagcc ctcgcagttg aggcaaaaac cgatgatttc cctgagagtg agatcaccat
    27061 tcggattgtc gatgaagcag aaagccacga gctaaatctg acttatcgtg gtaaagacaa
    27121 gccaaccaat gtgctatctt tcccgtttga ggcccccgaa ggaatcgaaa tgccactatt
    27181 gggtgattta gtcatctgtc gtcaagtaat ggaaaaagag gcggaagaac aaggtatttc
    27241 agccgaatcc cattgggctc atttggcgat tcacggcaca cttcatctgc tcggctacga
    27301 ccatattgag gaagatgaag cagtggaaat ggaaagcctc gaaaccgaaa ttatgcaaag
    27361 tttagggtat gaagatccgt atctttcaga aaaaatatag tcttgtaggg gagggtcctg
    27421 tgccctccag tactccatta taatcggaca ggcacaggag ctgttcctac aagttatatg
    27481 agaacgatcg caatcataac atctcctaac aatgccgact ttattcaaca aggctatttt
    27541 tacattcccg aaaatcatca gcttaaagct atcccctttt ccaatgccaa aacgatttta
    27601 ggacaagaat acccttttgc catttatact atgcaagcag aaaatggggt gaattttaac
    27661 ttagatgctt ttgcaatact tgccggcact attcaagaaa acggtacttt atttttgctt
    27721 tgtcctcagt gggataattt ggaaaatgaa ttagatttcg atgcgttacg ttggaacgaa
    27781 aatcaggcta ttgcctgccc gaatttctat caacatttta aacaattagt cgctaaattt
    27841 ggttttgaag tcagaactaa tttgccaaaa ttaccggcta caagcggtca aattccttca
    27901 aaaatttacc aattaacgca agaacagcaa aatattttgc aaaatctacc gcttgatcct
    27961 gccgatattc atttgattac cgcccctcgt gggcggggca aatctaccct tgcagggcga
    28021 cttgcaaaag cgttacccgc acaaaataag gtattaatta ccgcacggtc ttattctgca
    28081 ttgcccagtt tttggcgttc ggcagagcaa aaaatccctt ttttcgcacc tgacaattta
    28141 atcgagcaag ttaaaagtaa aaacatttca cctaaccaat ggctctttat tgatgaggca
    28201 gctagcctac ccttgccgat actccacaca ctttgtgcct attttgaaaa agtggtttta
    28261 accacaacaa cccaaaatta cgaaggcacg gggcgaggat ttgagctgaa attcccggta
    28321 atgttggata aaccgtttaa acattggacg ttatctcaac cattgcggtg gagcgaaaat
    28381 gatcctctcg aagcatttgt gactgagttg ctattacttt ccgaagaaaa atctcccctc
    28441 tttagcaaag aggggaaggg ggagatttat tcgcttcgct cacactatgt gccgttgcta
    28501 aagcaacgtt caaacgcaaa gcgtttgtgg cagaagcaat tatcgaagaa tataagttat
    28561 tcgaataagc atttacagga aaaaatcgct ttctaccact tactcgccaa cgcccattac
    28621 aaaaccactc caactgactt acgccgttta tttgatggcg aaaatcagct tttgttttca
    28681 caacaggaaa acgagcaact tatcggcgga atttgggcaa tgcaagaggg tggtttagat
    28741 gaaaaattaa cccaagccat ttggctaggg gaacgccgtc cgcaaggcag tctagtggct
    28801 caatatctct gttttcaagg gaatttaccg caggcttgca aattgcattc cattcgcatt
    28861 tctcggattg cggtattgcc tgaaaagcaa aatcaaggcg ttggcaagcg gttgatttct
    28921 cagtttattt tgcaaaattc ggtagaaaaa cgaccgcttg attttatctc tgtgagcttt
    28981 gggctaacta ctccactcca cgcattttgg caatcttgtg gtttccaact tgtgcaaatc
    29041 acaccgaata aagaggcaag cagtggttat caaagtgcaa tgatgattta cccgatttcg
    29101 gagcaaggaa agttatttgc acagcaggca acccaagcct ttgccagaga tttcgccctt
    29161 cagccatttt tcacggaaat ttgtgaggaa ttgcaaaatt tcgaacaatt tcgaccgctt
    29221 gccacacaca atttagatga acgtgattgg aaaaatttaa ccgcgtttgc tcatggcaag
    29281 cggacgtttt cagcggcgta tgtgagttta aaacggcttt atttatcgga taaagcacgc
    29341 ttttcagagc tggatatttt tgaacgaaaa gggcaaaaaa tctccaaaat ggaggtggaa
    29401 aattatcgaa aactcgtttt gcaaaactta aacaataaat catttaaaat aatttatagg
    29461 ttttattaca tttttttgtg tttttgctct tgctaaatct gcaatttaat cgtataaatt
    29521 cactgtttat ttttcatttt gatgaggaac tccaatgtca atgtttagtc acattcaagc
    29581 tgctccggca gactcaattt taggcttagg tgaagcgttt aaagcagata gccgtgaagg
    29641 taaaatcaac cttggtatcg gtgtttatat gaccgatgaa ggcaaaaccc cgattgtcaa
    29701 agcggtaaaa gaagcagaaa aacgtttatt agagacggaa aacagtaaaa attatttaac
    29761 cattgacggt gttcaagcat tcaatgcggc aacgcaagcg ttattatttg gcgaaggggc
    29821 tgaagtgatt acctcaggtc gtgctaaaac tgcacagagt ttaggtggga caggggcatt
    29881 acgcattgcg gcggaattta tcaaacgtca tacgaatgcg aaaaatgtat ggatttcaac
    29941 cccgacttgg ccaaaccata acggcatttt tgatgcagta ggcgtgaatg taaaaggcta
    30001 tcgctactac aataaagaga ctaacggttt agattgggaa aatttaattg ccgatttaag
    30061 ccaagcggaa gccggagatg tggtgttatt gcacggttgc tgccacaacc cgaccggaat
    30121 tgacccaaca cctgcacaat gggaacagtt agcagcatta tctgccgaaa aaggctggtt
    30181 gccgttattt gactttgctt atcaaggctt tgcaaacggt ttagaggaag atgcttacgg
    30241 tttacgagct tttgtgaaaa ataaccgtga attgttggtc gcaagctcat tctcgaaaaa
    30301 ctttgggtta tataacgagc gtgtcggtgc atttacccta attgcagata atgctgatga
    30361 tgccaaccgt gctttcactc aaatcaaatc gattattcgt gtactttact ctaacccatc
    30421 tgctcacggt gcaagtgcag tagcagttgc attggctgat cctgacctta aagcacaatg
    30481 gattgcggaa ttagatgaaa tgcgtaaccg tattaaagaa atgcgtagcc aattagtgca
    30541 gaaattaaaa gaaaaagggg caaacaaaga cttctctttc attactgagc aaaatggtat
    30601 gttctcattc agtggcttaa caccggagca agtggataag ttaaaagatg agtttgcaat
    30661 ttatgctgtg cgttcaggca gaatcaatgt agcaggcatt accagcaaaa acattgatgc
    30721 tcttgccgat gcgattgtga aagtgcttta attaaacctt aaagatatat agaggcataa
    30781 gcctgagtgc ttgtgcctct tttttatctt ttaggtacaa gtgtggacac ttgcaccaac
    30841 gttgattatt cgtagaacta ggtttccaac caaagcataa aatctgctac cgtatgaaat
    30901 gagccgaaca ccagcacaat atcattttcg ttggctgttt caaacaatac tcggcttgcc
    30961 tgtgccacat tttcatagct acaagcggtt gaatttggca aaacgtttac caatttcgct
    31021 aacaccgcct cgcctgtttg tccacgccaa caatctagcc ctgcacaatg ccattcatca
    31081 ataatattag ctaatggctc gacaataccg gataaatcct tatcttctaa cgcactaaac
    31141 accgcataga ttttttgatt tggttgcttt aactcccgca gtttgtcagc taaataacgt
    31201 gctgcgtgtg ggttatgccc tacatcaata attacttttg cagccggttt aggttgagaa
    31261 aaatgagtaa aatcggtatc cgtcagggtt tgaaaacgcc ctgtcatttg agcttcattt
    31321 aaggcttgtc gaataatctt ttcatcaatt tcaaatggca gttgcattaa tactgctaat
    31381 gcggtgccgg cgtttggaat cggaataagc gggattggta aatcggtaaa ggatttttct
    31441 tcagaatacc aatgaaacag attattctta atttcaaact gccagtcttt accccggtaa
    31501 aaagcgtgac aatttaacga atcagcaatc tgtttaatcg aatgagggca atcaggctcg
    31561 ccgatgacaa ccggaatatt ttcacggaaa atgccggctt tctcacgccc gatgtcttca
    31621 cggttatcgc ctaaaaatgc aacgtggtcg atatcaatcg agataatcac ggcaaggctt
    31681 ggatcaataa tattagtggc atctaatcgc ccacctaatc cgacttctaa aatcacaata
    31741 tcgagctgat tttgtttgaa tagctcaaat gcagacagcg tactaaactc aaaataagtg
    31801 agtgaagcgg tcttttttgc attaattttt gcaaatgctt ggacgtggtc ggcatcaggt
    31861 aatacttcgc cgttaattcg cacccgttca ttatagtgaa ttaagtgcgg tgaagagtaa
    31921 acaccaaccc gaaatccggc ttttaataag gtaacttcta acaaacggca ggtcgaaccc
    31981 ttgccgttag taccggcaac ggtaatcaca taaggggcag gcttgagtaa atccagctct
    32041 gttgcgacct tacgaatacg atctaagccc atatcaatcg gtttagaatg ggcttgttct
    32101 aaataggaaa gccacgtttc caaagaatcc gtggctttag gtatgcttaa tgtattcatt
    32161 cagatatttt attctgcgtt ttctacaacc aattccgcca ctttaaacgg cgttggttga
    32221 ttggttaatt ttgcacaaag acgggaaagg gtatcacgca tctctttacg atgcacgatc
    32281 atatcaactg ccccgtgttc taataagaac tcggcacgtt ggaaaccttc cggcagtttt
    32341 tcacgtacgg tttgctcaat tacacgagga ccagcaaagc cgattagggc ttttggctca
    32401 gcaatattca catcgcccag cattgccaaa cttgcggaaa ccccacctaa agttgggtcg
    32461 gttaatacag aaataaacgg tacgccctgc tctttcattt tggctaaaat cgcactggtt
    32521 tttgccattt gcattaaaga gaataacgcc tcttgcatac gagccccgcc cgatgccgaa
    32581 aaacagataa acggaatttt ttcagctaaa gctttttccg cagctttcac aaattttgca
    32641 cctactacag accccattga accgcccata aactcaaagt taaaggaggc cgtaaccacc
    32701 ggcatattat gcaatgtacc ggcaagcacg ataaaagaat ctttctcacc ggtttgtttt
    32761 tgcgcggcag ttaaacgatc tttatatttc tttaaatctt taaatttcaa cacatcttcc
    32821 ggctctaagt cggcagcgat ttcgaccgca gaatctttat ctaacaaatt caataaacgg
    32881 gtgcgggcat caatacgcat atgatgatcg cattttggac aaacctgttg gttgcgttgt
    32941 aattcttcgc tatagagaac ttgttcacaa cttgtgcatt ttgtccatac accttccggc
    33001 acttttgatt ttgcattgct tgatgatgaa gagtctctac ctaaaattct gtctaaccag
    33061 ctcattatta ttccttttgg attgagttgt tgggttcact aaatggttgc tattaaagca
    33121 taatttatcg gtttttgata tgttttcttg acatatcaga tcactaacta tgtgtataag
    33181 ttcgataaaa aaacgcaatc agaagaatga ttaacatcac cacaatttga attaagcgtt
    33241 ttttggttaa tttttccgct gccattgctc tgctcatact taataaattt ccgcattata
    33301 agcgaaaaag cacgggctat gtagaatttg caaaattttg atcaaaaata accgcttgta
    33361 aaaaggaaat actatggcaa ccattgaaca atttcttgag gttgtaaacc aacttcgtca
    33421 ccctgaacac ggctgcccgt gggatctcaa acagaatttt gacacgatgt tccccactta
    33481 ttggaagaaa cctacgaagt agcggaagcg attcacaccc aagatcgcac tgccttgcgt
    33541 gaagagttag gcgatttatt actgcaagtg gtatttttaa gccaattagc gaaagaggaa
    33601 ggcagtttca cttttgatga tgtagtaacg gatattcacg ataaactgat ttaccgccac
    33661 cctcacgttt ttggtgatgt gaaagctact aacagcgaag aggccttaca aagttgggaa
    33721 gcacaaaaag cgaatgatgc aaaacaccgt agccaagatt ctatttttag atgatttacc
    33781 ttttgctttg cctgctctta cccgtgcaaa taagctacaa aaacggtgtg caaaagttgg
    33841 attcgattgg gataaccctc acgatgtgct ggcaaaagtc gaagaagaac tcgatgaagt
    33901 caaagcagaa atcgcacaat atccggctcg ctccatcgag cttgctgaag aattaggcga
    33961 tctcttgttt gcgattgtaa atttatgccg ccattatcat accgatgccg aggaaatttt
    34021 acgcaatgca aatctaaaat ttgaacatcg ctttcgaaaa gttgaaaaat ctgtgaaaaa
    34081 atcgggaaga actgtaaaag aatgtaaatt agaagaacta gatgtaattt ggcacaaaat
    34141 aaaagtggta aaataaaaag ca
//
LOCUS       DANLRK010000006        31352 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.006, whole genome shotgun sequence.
ACCESSION   DANLRK010000006 DANLRK010000000
VERSION     DANLRK010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 31352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31352
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.006"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            248..976
                     /gene="ttrB"
                     /locus_tag="PXA33_000195"
     CDS             248..976
                     /gene="ttrB"
                     /locus_tag="PXA33_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetrathionate reductase subunit TtrB"
                     /protein_id="HDL5119576.1"
                     /translation="MDLSKRSFLKELSALTVGASFVPLQSAQAFMPAWREGDENKRYA
                     MLIDLRKCIGCQACTVSCSVENATPLHSFRTTVRQYEITNGTQVVNNVVLPRLCNHCD
                     QPPCVPVCPVQATFQRKDGVVVINNEQCIGCGYCVQACPYDARFINEETKTADKCTFC
                     THRLEVGLLPACVESCVGGARVIGDLNDPKSQISQLYQAHKDDLKVLKPEAGTVPHVF
                     YVGLDDDAFVSKIDGQPMLWTGEA"
     gene            978..2009
                     /gene="nrfD"
                     /locus_tag="PXA33_000196"
     CDS             978..2009
                     /gene="nrfD"
                     /locus_tag="PXA33_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HDL5119577.1"
                     /translation="MIREVLVEPQHIVWLPWIVHYFFFVGVAATAVFTAVLFAKKSLK
                     PTACELAAVTVALIGSIVAPVALTADLHQPSRILHFYTDFAWRSPMAWGAIILPLFSV
                     AVAGYFVLALAHHTRPNLPKWLAWLQLPILQNQGLLWAFRLFATLTAIGIIGYTVLET
                     YQTGTRTLWHSAWLLPIMLFSAWAVALGLTQAVCKMLFHTQTNTGLWTKLCLILTALS
                     LVGLAFSSPQATQDFAQLFNGSITAYLVGIFWLIALICNFSAKNHRLQWLGVLALIAF
                     GWLLRWVLVIQVQTIAKTNALQNPYHFDWTAVDGGLGIVSIFGLTVLVSIVVWQLILI
                     ICGGRKGEQ"
     gene            1999..5094
                     /gene="ttrA"
                     /locus_tag="PXA33_000197"
     CDS             1999..5094
                     /gene="ttrA"
                     /locus_tag="PXA33_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetrathionate reductase subunit TtrA"
                     /protein_id="HDL5119578.1"
                     /translation="MNNKRRNLLKGGLALGGAAAFGAGYSPKVSEIAKGVMHGTSGEK
                     TRDAIHGNSLTPEYQVKDGKLLSNPEQIVCNTQCMGCWTQCGIRARVDLNTNQVIRIS
                     GNPYHPLSSDKTLPFGMPIAKAELLLAGESGIENRSTACARGAAFLEGVNSPYRITQP
                     LKRVGKRGEGKWQTISFEQLVEEIINGGNLFGEGEVEGLKTIRNLVDPINPKQPDLGM
                     KANQLLVTFAGPEGRQPILQRFAQKSFGTINFSNHGAYCGAAYRAGSGAFMKDFDNQQ
                     HAKPDWDHAEFILFIGTSPAQSGNPFKRQARQLAKRRPDDNFSYVVVSPRLEMTSSLA
                     TQNNHWVPIKPEGDLALVMGMLRWIIENERFNADYLSRPSLAAMENAGHVSYANASHL
                     IICEPNHPKFGQALRISDLQDVQLDPEEKVEENLIVVKDQASGELISAKDCLQAVIFV
                     EQIAKLLDGSEVLVKSSMQLLKEATQEKTLAEYSDICGVPVQTIENLAKKFTSHGHKA
                     NAITHGGTMHSTGFYTSWAILLLNAMVGNLNKKGGMAVAAGKFKDFGDGPRYNLANFP
                     NEVKPKGTNLARSKKAYQNSSEFKQKVTKGENPYPAQGAWYPFTGGQTTEMFTSMVQG
                     YPYFAKAWINHMANPVYGLTAMNPIALEKLKDPKILPLFVSIDAFMNETTALTDYIVP
                     DTHNFESWGFSTAWGGVPQRTSTARHPIVPSKNAKTAEGDTICMENFVIAVAKKMGLP
                     GFGENAIADLEGNRYPLHRTEDYFLRAAANIAFAGEKPAPDASEQDLLLTGVQRIMPK
                     IEQTLKAEERLKVANVYCKGGRFAPHESAWKEENMEFQWKNCLQIWNENVYKAQHHSN
                     GEHYWGCPRYMSPRFADGSTLEQHYPQSEWGFKLISFKSNIMSSITAPLLRLLSIKPE
                     GLVAMNRLDAEEKGIKHGDVVKLSTPTAGLTVQIVVLDGIARGTIGIEHGYGHKQIGA
                     SSYTIDGVEIPANPMIAKGINLNDLGIIDHTKAVKSPWVDWVCGSAVRNGIPAKVEKL
                     A"
     gene            5235..6080
                     /locus_tag="PXA33_000198"
     CDS             5235..6080
                     /locus_tag="PXA33_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL5119579.1"
                     /translation="MTFVKKTALSLIATALFATSSFAAEQSQYQHIRNATAKIEYAGK
                     TFLVDPYLAEKDRYLPPNAKKRNPTLDMTVSIDEVLNGVDAVIVTHTHHAHWDDVAQQ
                     RVPKNLPIFVQNAGDARLIRSQGFTDVRVVGKDTPFEGVTLTRTRGQHGTDAMYANPK
                     IAEMVGDAMDVVMKAEGKPTVYLVGDTIWNHEIEHSFNTYKPDFVVLNAGCAKLPNFE
                     GSPIMCTEDVSKAHKIAPQAKILTVHMDALPQTSISSDQMRDYVQKEGLNRVTVPKNG
                     DVIKF"
     gene            complement(6193..7590)
                     /locus_tag="PXA33_000199"
     CDS             complement(6193..7590)
                     /locus_tag="PXA33_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HDL5119580.1"
                     /translation="MKLSKLAFSVALALTVTACTTGNMSNDGTLQQAQQTYQSYDEIT
                     KQYQINEQWWQGYTDAELNRVVDLAIKNNLDLAKSGIAVNKALYNANLVGANLVPTFS
                     GSGSVSAAKGLGSASSNNVSTGVSTTAAQAAFNLSYTVDLWRRLADTASAAEWTHKAT
                     IEDLKATRLSIINAVVTTYYQLAYYRDAIRITEESIRHYEQISRILTNKMNAGAIDSL
                     AVDQAQQAVLNARNSVIDLKTNQKIAEQTLRNLLNVQPNTPLNIRYPNIMSVKLQGVD
                     LNVPVSAIANRPDVVGSLQRLQSAFKTLTATEKSWFPTLTIGGSITGSATNIGNVADN
                     PVGNGLIKLDLPFLDWNRVKNNVKISETDYVTARLNYEQKITSALNEIDRYYYSYTQS
                     RSSLKILQDTNAKNKRISSYYKNRYEQGVSEFREWITALNTELNSQLAILNAKYAILT
                     NENAVYQAMAGKYRR"
     gene            complement(7680..8303)
                     /gene="gmk"
                     /locus_tag="PXA33_000200"
     CDS             complement(7680..8303)
                     /gene="gmk"
                     /locus_tag="PXA33_000200"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HDL5119581.1"
                     /translation="MSLGNLYIISAPSGAGKSSLINALLADLPRSEVQLSISHTTRQP
                     RPAEQDGVHYYFTSHDEFKQLIEQGYFLEWAEVFGNYYGTSLPMIERSLEQGIDVFLD
                     IDWQGARQIREKVPNVKTVFILPPSKTELEKRLIGRGQDSAETIAKRMAEAVSEMNHY
                     NEFDYVIINDDFQQALAELKAILIAERLSLGSQQIRHQHLITDLLNN"
     gene            complement(8416..8889)
                     /gene="gpt"
                     /locus_tag="PXA33_000201"
     CDS             complement(8416..8889)
                     /gene="gpt"
                     /locus_tag="PXA33_000201"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDL5119582.1"
                     /translation="MSEKYTNEKYVVTWDMFHMHARKLAENLLPATQWKGIIAVSRGG
                     LFPAAVLARELGIRHVETVCIASYDHDSQGELNVLHAADTDGEGFIVVDDLVDTGNTA
                     KEIRRMYPKARFVTVFAKPHGAPLVDDYVIDIPQETWIEQPWDLGITFVQPLARR"
     gene            complement(9035..10444)
                     /locus_tag="PXA33_000202"
     CDS             complement(9035..10444)
                     /locus_tag="PXA33_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HDL5119583.1"
                     /translation="MKKYPLLFSLFSPVVWAEKVIEHNTDPISFERQPQAVKFEQNVV
                     KNSQKPTAYQPAYSVNQNVEQQINQAILSQDWATLEPLLSRYPQTENFDPLLWHYAQA
                     ALAYAKKDHQTAVFHYRQLLAKPPSLVYPRFDLALILAEDQQFRAAEQEFKAIQAHLP
                     PELAKIARNTQVQIAENEAWQPDFYLQYTQTDNVNNASSSPIVNVNGRIFRKNADSLP
                     QSAKGVRYGLGLSKLHNVKGNHFVGLTLNYSGIYYWDNRDYREQSVSVSPNYSYRTVA
                     YRLTFSPFIEQNWLGSSRYNVQFGATLNGLRYLNSQWLVSGSFTHLQKRYFEELTASR
                     YNSYQHQLSGGVQWQAVKNGRFFANLDGVREIAREKAQSSNKWLARVGAVFQGERWAT
                     QASIGFGKRYFADKHYLFGYKRLDTEYQANLAVWNRTWHWQGIVPKLNFRYQKIDSNI
                     ADFYSRQNKEVFFTLEKLF"
     gene            complement(10640..11770)
                     /locus_tag="PXA33_000203"
     CDS             complement(10640..11770)
                     /locus_tag="PXA33_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin transporter"
                     /protein_id="HDL5119584.1"
                     /translation="MSIKKVTLASLILLGLTACSSGGSGSSSNNVAKPNTKPQTTQQQ
                     TDKAAAEKAAKDKAAKEKAEAERLAAEKAAKEKAEAERLAAEKAAKEKAEAERLAAEK
                     AAKEKAEAERLAAEKAAKEKAEAERLAAEKAAKEKAEAERLAAEKAAKEKAEAERLAA
                     EKAAKEKAEAERLAAEKEVDPIAIIKAKGIDDTNYGLKLGNSKETIEGKVTVRTQSYL
                     YNQPYSVVTAQAVQRNGWENGNYIDKVDGTVTVKGLKTEKLPTEGTATYSGKAFNHDG
                     AKGFNGGDLTYNVNFSKRTGSGRVEGEFGNLIVLEEGTINNTQISSTVHGNNPLNLSG
                     KYTVEFFGKNAEEIAGEIQTKANLTGEVSNFGLAGTRGDIQK"
     gene            11973..13427
                     /locus_tag="PXA33_000204"
     CDS             11973..13427
                     /locus_tag="PXA33_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HDL5119585.1"
                     /translation="MSEISTLAPQLLWQWFDKACAIPHPSYHENELAEFIVNWAKSKN
                     FFVERDEVGNVLIRKPATKGMENRQTVALQAHLDMVPQANTGTVHDFTKDPIQPYIDG
                     EWVKAKGTTLGADNGIGLASCLAVLDSDDLAHPTIEVLLTMTEEAGMEGAIGLRPNWL
                     TADIMINTDTEENGEIYIGCAGGENVNVTLPIFLVPHQQDSAVTVTLKGLNGGHSGCD
                     IHTTRANAIKVFARILEQAHNEVEFQIAEIQGGSVRNAIPREAHATLVFSAKNQQKLT
                     ACLSQTAEQVKAELKIAEPNFQFLIEEAQLPEQAFDLASTARVIHFLNVMPNGVVRNS
                     DVVENVVETSLSIGVLNTEASEVKATILARSLIEGGKEEIRSKIRSLTALVGAEVEFS
                     GNYPGWEPNPNSKITPLTKAIYDEILGYESQIKVIHAGLECGLINKIYPNMDFVSIGP
                     TILNAHSPDEKVHIPAVEIYWKLLTKLLEQAPEK"
     gene            complement(13502..14791)
                     /locus_tag="PXA33_000205"
     CDS             complement(13502..14791)
                     /locus_tag="PXA33_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL5119586.1"
                     /translation="MDIRQKIAHNKMTSFQWLVVGLTVLLNMLDGFDLMALAFTAKSI
                     REELQLDGAAIGSLMSAGLFGMAIGSLFLSPLADKFGRRPLLLIGTGLATLGMLLTYF
                     ATSAFEIGVWRVLTGLGVAAILTGTNVLVSEYSSQKWRGLAIAIYASGFGIGAMLGGM
                     SAVLLQGEYGWRAVFLVGSILTGAVFVLLFFVLPESVEFLISKHPVNAKARLNQIAVK
                     LGLGNGWELPEIEAKSKSKVAITRLFEPEYRRNTLLIWLAFIAISASFYFISSWTPAL
                     LEEAGMPKTQSQTVGMAISVGGTIGSLIFGFLVSRWAAKHILQAFSILAASAVVAFVF
                     AGNLGLAITLAILVGGLINGCITGLYTINPTLYAPDFRSTGVGTAIGVGRLGSIVSPT
                     LAGILLDAGWKKNELYLGAAVVLLIAAFTVHFLKQRK"
     gene            complement(14995..15330)
                     /locus_tag="PXA33_000206"
     CDS             complement(14995..15330)
                     /locus_tag="PXA33_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HDL5119587.1"
                     /translation="MVSEQRQNPEEQKSEEQKINVEVLYAYPDKYFLKKLQVPQNATI
                     QTIILLSGVLEKYTEIDLRENKVGIFSRPAKLNETVEEGDRIEIYRPLIADPKEIRRK
                     RAAEQAEKK"
     gene            complement(15320..15754)
                     /locus_tag="PXA33_000207"
     CDS             complement(15320..15754)
                     /locus_tag="PXA33_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HDL5119588.1"
                     /translation="MPIVNQSALVPYSAEQMYQLVNQYEKYPEFLSGCVSTKTLSTGE
                     NELKAELHIQKLGISQTFSTHNKMTPPYKIEMALIEGPFRHLHGAWTFTPFDEQSCKI
                     ALQLNFEFSSPIVAMVFGKVFNELTLKMVNAFKQRAKEVYGV"
     gene            complement(15809..16864)
                     /gene="modC"
                     /locus_tag="PXA33_000208"
     CDS             complement(15809..16864)
                     /gene="modC"
                     /locus_tag="PXA33_000208"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HDL5119589.1"
                     /translation="MLKLKLLQQLGNLTLNVNLDLPNKGITAIFGRSGAGKSSLINLI
                     AGLSTAQAGFIELNGQVLFDSQNGINLPPEKRNIGYVFQEHRLFPHYSVEKNLKYGYK
                     RSDSADFLQIVELLGIAPLLNRYPASLSGGEKQRVAIGRALLSSPNILLMDEPLSALD
                     LPRKQELLDYLDKLSARLEIPILYVSHSLEEVVRLADHLVLLENGKVTAFDTLSAVWH
                     SSAFSAWQPNHQRISLLELPVEETLPHYNMLGLSLNAQRLWVNQLPRHQIGEKLRVTI
                     ASKEVSISLQKPTQSSIRNVLKGNVRKIETHLDRVDLAIQVENQQIWSTISLWSFNEL
                     AIQPEQEVYLQIKSVSL"
     gene            complement(16851..17543)
                     /gene="modB"
                     /locus_tag="PXA33_000209"
     CDS             complement(16851..17543)
                     /gene="modB"
                     /locus_tag="PXA33_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HDL5119590.1"
                     /translation="MLNFTPQELNAIVLSLKVASIAVVLALPLAVWVAWLLARKQFWG
                     KNLLNGIMHLPLVLPPVVIGYLLLISMAKRGVIGQYLWEWFHFSLSFSWKGAVLASMV
                     MAFPLMVRSIRLAFEAVDPKLEQAARTLGAIPLKVFFTLTLPLSFSGIIAGGVLGFAR
                     SLGEFGATITFVSNIPNQTQTIPAALFTFIETPDGEMAAARLCLVAILISLIALFCSE
                     WLAERQKRYAQT"
     gene            complement(17727..18482)
                     /gene="modA"
                     /locus_tag="PXA33_000210"
     CDS             complement(17727..18482)
                     /gene="modA"
                     /locus_tag="PXA33_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL5119591.1"
                     /translation="MKALKKLAITMALTLSGTAFSQEITVFAAASMTNVLQDIQQDFE
                     KAYPQDKITFSFASSSVLAKQIEQDAPADIFISADQKWMDYVAEKQPEKVKHRRNLVR
                     NELVLIAPTESNIEPKAIQAVDFAKELANSYLSVGDPNHVPAGKYAKKALSHYQLWES
                     VEPRLARAKNVRDALSFVERGESPLGIVYSTDAKVSNKVKVVAVFPPESYGEIVYPAA
                     AVSEKASAATFLDFLKTPKAKAKFEAAGFYPVN"
     gene            complement(18503..19264)
                     /locus_tag="PXA33_000211"
     CDS             complement(18503..19264)
                     /locus_tag="PXA33_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007523083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL5119592.1"
                     /translation="MNLIGFYTLAYKECRRILRIWRQTLVPPVITTTLYFLIFGTLIG
                     GRIGEMNGVGYTQFIAPGLIMMTAITASYVNTASSFFLSKFTRDFEEMLISPLSTHNI
                     IWGYVTGSILRAGLAATLVMCVAMVFVSFEIYSVAIIVLTLLLTIIAFALGGVINAVF
                     ARSFDDVGLIPTFVLTPLTYLGGVFYSISLLPEFWQTVSKFNPIVYMINGLRYGFLGI
                     SDVPLGYTFAVLTSLCIGLYIIAYSLIQRGVGLRS"
     gene            complement(19266..20174)
                     /locus_tag="PXA33_000212"
     CDS             complement(19266..20174)
                     /locus_tag="PXA33_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL5119593.1"
                     /translation="MKALELIDLVKTYPTGVKAVKGINLVVEQGDFYALLGHNGAGKS
                     TTIGIISSLVNKTSGSVKVFGYDLDTQKVELKHQIGLVPQEFNFNQFEKVIDVLVQQA
                     GFYGIPRSEALVRAEKWLKLLDLWEKRTHLTRELSGGMKRRVMIARALMHNPKLLILD
                     EPTAGVDIELRRSLWDFLRDLNNQGTTIILTTHYLEEAETLCRHIGIIQHGELIENTS
                     MKALLAKLESEVFVLDLQNSEQNQPLVIQNYPHKWLDENTLEVNVKRQQGLTNLFSQI
                     SAQGAEVLSMRNKSNRLEELFINMAQ"
     gene            complement(20180..22237)
                     /gene="dnaX"
                     /locus_tag="PXA33_000213"
     CDS             complement(20180..22237)
                     /gene="dnaX"
                     /locus_tag="PXA33_000213"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HDL5119594.1"
                     /translation="MSYQVLARKWRPQRFSEVVGQQHVLSALENSLREDRLHHAYLFS
                     GTRGVGKTSIARLFAKGLNCVNGITAEPCGECANCKAIEEGHFIDLIEIDAASRTKVE
                     DTRELLDNVQYKPTVGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEYVKFLLATTDPQ
                     KLPITILSRCMQFHLRALEQSQIARHLEFILNAENIPFEPLALEKLAKAAQGSIRDSL
                     SLTDQAIAMSNANITLAIVSQMLGLIDDNQPLELVQATAQADGEKAMAIIEQIAAKGV
                     DWQQLLSDVAEVLHKIAMLQLVKTPTTEETLVHFLAKQIPPEDVQFFYQLILNSKKEL
                     PFAPEQRAGVEMAILRALAFHPKQAEKIATVAVEKVTQPNHSVSAVTTAKPQNQISQL
                     RENFEQNQQKTVTVANTTTAVESIAQPPQAVDTAISPSAPATLSPAMAALEARKKLQA
                     QQANLAQTEKKKPELKAPQVIAKKSANQTASLQERFISLAENQHKAVLQVSKKTESAP
                     KSVEDEDYRWTWLNPELENQADSAKPSDIKQAILQERTPELVAKTIHLSCQQDKWCDI
                     ANSLNLGGFSRQIALNSYLAEQNEQHLALVLKPNMAHLDNPESRKNLEAALAEKGYSY
                     ELTIGDNSEQQTPLEIRRAIFKQLTEAAKSALLNDEKLMLLRNAFDAQIDESTIRAVA
                     ETE"
     gene            complement(22328..22867)
                     /gene="apt"
                     /locus_tag="PXA33_000214"
     CDS             complement(22328..22867)
                     /gene="apt"
                     /locus_tag="PXA33_000214"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HDL5119595.1"
                     /translation="MNQLDLIKSSIKSIPDYPKAGIIFRDITSLLEVPTAFQATIDAI
                     VAEFKDKGITKIVGTESRGFIFGAPVALALGVPFVLVRKPKKLPREVISQSYTLEYGE
                     DTLEIHKDAVSANDNVLVIDDLLATGGTIDATAKLIRRLGGNVEHAAFVIWLPELGGK
                     ERLEKEGVKSFSLVSFEGH"
     gene            complement(22995..23945)
                     /gene="lpxM"
                     /locus_tag="PXA33_000215"
                     /gene_synonym="msbB"
     CDS             complement(22995..23945)
                     /gene="lpxM"
                     /locus_tag="PXA33_000215"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249087.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HDL5119596.1"
                     /translation="MTRITAQDGYTHKFLWSFLHPKYWGVWLGVLGLVILAYVPVRIR
                     DKLSAFVGKMAYRYLKQKNKKGYHRAKVNLRYCFPDWSEKKREEVVEKMFITVAQTML
                     AIGETAIRPASYLQKRCEFTGFENVVKAKESGKNVIMLVPHTWSIDMAGVAMFSLGYP
                     MTSMYNPHRNALVDWLWNITRERLGGRMHTRQNGIKAFLADVRKGDVGYFLPDEDFGE
                     ELSVYADFFATEKATLPGLNKMARVANAEVIPMFSIYNAEKAKYEMEILPPLEFSGDP
                     IQSAREMNRVIEYFVEKNPEQYVWILRLLKTRRNREDIYL"
     gene            complement(24386..25069)
                     /locus_tag="PXA33_000216"
     CDS             complement(24386..25069)
                     /locus_tag="PXA33_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005624031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HDL5119597.1"
                     /translation="MEREQQIPVTNIDVAPKQSSVWKTIFADGVWKNNGALVQLLGLC
                     PLLAVSNSVTNALGLGLATMFVLICTNITVSLFRKITPHDIRIPIYVMVIATVVTAVQ
                     LLMNAFAFPVYQSLGIFIPLIVTNCIVIGRAEAYASKNEVKFSAFDGFSMGLGMTFSL
                     VVLGALREIIGNGTLFSGLDLLLGDWAKALRIDVLHIDSGLLLAILPPGAFIGLGVIL
                     AVKNIIDKR"
     gene            complement(25078..25710)
                     /gene="rsxG"
                     /locus_tag="PXA33_000217"
     CDS             complement(25078..25710)
                     /gene="rsxG"
                     /locus_tag="PXA33_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HDL5119598.1"
                     /translation="MNTSKITLNYALILGFIALLCTAVSTGVYLLTKGKIDDVTAMQQ
                     RKLLEEVVPKSHFDNDVLATCKLVQLENAPYLNRIYVAKKSENLTAYLVQGTAPDGYS
                     GDIVLLMGIEPNGNILGVRTLEHKETPGLGDKIETRVSDWILSFTNQLFSLENEADWN
                     VKKDGGKFDQFTGATITPRAVVNHIRQSAKWVVTELAQNPTMFNQFKACK"
     gene            complement(25710..26765)
                     /gene="rsxD"
                     /locus_tag="PXA33_000218"
     CDS             complement(25710..26765)
                     /gene="rsxD"
                     /locus_tag="PXA33_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HDL5119599.1"
                     /translation="MFKMVSSPHTHSSNLTANFMLWVIAMMLPAFGVQLYYFGYGVLI
                     QTLLAVSLAIVIEIGIAKLRKKTTAFYLADLSGILTALILAMSIPPYAPYWLVLIGVI
                     VALLLAKHSYGGLGQNLFNPAMVGYALLLVSFPVQMTAWIVPIELLSEPPTFRDAISL
                     IFYGVTSDGFSIHQLVNSVDGIAQATPLDSMKSSLAKSGFDAVMNTPIFNGLFATGWL
                     QLNLAFLFGGLVLIYKRIIHWQIPVAMLATFAFFSGFTELFSQQSHLHFVSHFFSGAM
                     MFGAFFIATDPVTASITPRGKLIFGGLVGLLVYLIRYYGNYPDAIAFSVLLANICVPL
                     IDHYTQPRLYGTKLDNR"
     gene            complement(26811..27446)
                     /locus_tag="PXA33_000219"
     CDS             complement(26811..27446)
                     /locus_tag="PXA33_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HDL5119600.1"
                     /translation="MFSELIPALPWSFLFIQDLIGTIVFAISGAIAARQHKMDIFGMF
                     ILAFVTGVGGGTLRDMMIGSTPVFWMKQPIYVLMITLAVVITAIFRNQISRKSWQTGL
                     LVFDAIGLGVFTVIGVQKGLDFGLHPLICVALGGVTGCFGGLIRDILRNEVPIILQKE
                     VYVTASLIGGVIFVAANSVGLESDWVYLATVFTVIIIRLLAIRFNLNLPRL"
     gene            complement(27439..29733)
                     /gene="rsxC"
                     /locus_tag="PXA33_000220"
     CDS             complement(27439..29733)
                     /gene="rsxC"
                     /locus_tag="PXA33_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HDL5119601.1"
                     /translation="MSNPNLVIERIRQNKETGKLWDFPGGIHPMENKSQSNSKPIRTL
                     NLPKFFYVPLVQHSGWAGELLVKVGDVVLKGQPLTKGDHFRQLPVHSPTSGRVIGIEP
                     YVSAHASGLPEITVIIETDGKDKWVERQPIEDFLSLTSDQIIQKVYQYGIAGLGGAVF
                     PTATKLSLADKRCKLLVINGAECEPYITCDDRLMQDFTADLIEGIRILRYVLRPEEVV
                     IAIEDNKPHAIQALETALKGSNDMFVRAIPTKYPSGASDQLIQVLTGLEIPAGKRTIE
                     MGIVMHNVGTAFAVKRAIIDDEPLIERVVTLTGDKIRNKGNVWARLGTPIQHLLEQVD
                     YQADKRFPVFLGGPMMGFILPALQAPITKTANCLIAPDHFEYAPPEPERSCIRCSSCS
                     DACPVGLLPQQLYWFARAEDHDKSKEYHLDACIECGVCAYVCPSYIPLIQYFRQEKAK
                     IEEIEEKARKAEEAKIRFEAREARLQKERDARTARISQAAEKRREEVANAGGVDPVKA
                     ALERLKAKKAVETVQEIPAEVQVKPNGEPDNSDLMAQRRARRLAKQAEQAVEKSENVT
                     NALIGANNDLPLQSMNETASDKSVGANNYSPEAESEDPRKAAVAAALARAKAKKQAQQ
                     AVEKSENITNAVIGVNNDLPLQSMNETVSDKSVGANNHSPETESEDPRKAAVAAAIAR
                     AKAKKQAQQAVEKPEDIANAVIGVNNDLPLQSMNETTSDKSVGANNHSPKTESEDPRK
                     AAVAAAIARAKAKKAAQQEKSNNV"
     gene            complement(29720..30337)
                     /gene="rsxB"
                     /locus_tag="PXA33_000221"
     CDS             complement(29720..30337)
                     /gene="rsxB"
                     /locus_tag="PXA33_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HDL5119602.1"
                     /translation="MLDLPIIAYILIAIAVIALIFGAVLGYSSIKLKVESDPIVDQID
                     ALLPQSQCGQCGYPGCKPYAEAIANGDVITKCVPGGQPLVVNLAELLGVEVPPMEGMA
                     EPEMKVAFVIEDLCIGCTKCIQACPVDAIIGTNKAMHTIIPDLCTGCELCVAPCPTNC
                     IEMRPIKPTTQSWNWKFDQDLIIPIVNTTELQRKLVTGGGKNEQS"
     gene            complement(30337..30918)
                     /gene="rsxA"
                     /locus_tag="PXA33_000222"
     CDS             complement(30337..30918)
                     /gene="rsxA"
                     /locus_tag="PXA33_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007525505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HDL5119603.1"
                     /translation="MVDYILLVISTALINNFVLVKFLGLCPFMGVSKKVETAIGMGLA
                     TTFVLTVASLSAYLMETYLLEPLNAQFLRTLVFILVIAVIVQLTEMIVHKTSPTLYRL
                     LGIYLPLITTNCAVLGVALLNVNLANNLVESVLYGFGAAAGFSLVLVLFAALRERLAA
                     ADVPRPFQGASIALITAGLMSLAFMGFTGLVKL"
BASE COUNT         8708 a         7224 c         6763 g         8657 t
ORIGIN      
        1 attctaggag attagcgata cctagtaccc cgagtgatat taatgcgtat aaaaacttta
       61 gatctaaact aaaccatctt attgcttttt aattagtatc aatgccctat tatctacttg
      121 tatagggcat ttcccttcat ccttgaccca aatcaacttt ccccttgata tgtggaaaac
      181 cacaatacct tgccgttccc tctccgcctt agaatacctt tgttattttt aaatttgtgg
      241 gggaaatatg gatttaagca aacgttcctt tttgaaagag ctttccgcct tgacggtggg
      301 ggcgagtttt gtgccgttgc aatcggccca ggcgtttatg ccggcttggc gtgagggcga
      361 tgagaacaaa cgctatgcga tgctgattga tttgcgtaaa tgtatcggct gtcaagcgtg
      421 taccgtgagc tgttcggtgg agaacgccac gccgttgcac agtttccgca ccacggtgcg
      481 tcaatatgaa atcaccaacg gcacgcaggt ggtgaacaat gtggtgttgc ctcgtttgtg
      541 taaccattgc gatcaaccgc cgtgtgtgcc ggtctgcccg gtgcaggcaa cctttcaacg
      601 taaagacggt gtggtggtga ttaacaatga gcagtgcatc ggctgtggtt actgcgtgca
      661 agcctgcccg tatgatgccc gttttatcaa tgaagagacc aaaaccgccg ataaatgcac
      721 cttctgcact caccgtttgg aggtaggtct gctgccggcg tgcgtggaaa gttgcgtggg
      781 cggggcaagg gtgattggcg atttgaacga cccgaaaagt caaatttccc aactttatca
      841 agcccacaaa gatgatttga aagtcctgaa accggaggcc ggcaccgtgc cacacgtttt
      901 ctatgtgggg ctagacgatg atgcctttgt gagcaaaatt gacgggcaac caatgctctg
      961 gacgggggag gcgtaaaatg attcgtgaag tattagtgga accgcaacat atcgtctggt
     1021 tgccgtggat tgtgcattat ttcttcttcg taggcgtggc ggcaacggca gtgtttaccg
     1081 ccgtgctgtt tgcaaaaaaa tcactgaaac cgaccgcttg cgagctggcg gcggtgacgg
     1141 tagccttgat tggctcgatt gtcgcccctg tggcattgac tgccgacctg caccaaccga
     1201 gccgtattct gcatttctac accgattttg cgtggcgatc tccaatggcg tggggagcga
     1261 ttatcctgcc cctgttcagc gtggcggtag cgggttattt tgtgctggca ttggctcacc
     1321 atacccggcc gaacctgccg aaatggttag cgtggctaca gttgccgata ctgcaaaatc
     1381 aaggcttgct ttgggcattc cgcctgtttg ccacactcac cgctatcggc attatcggct
     1441 acaccgtgct tgaaacctac caaaccggca caagaacctt atggcacagt gcgtggctgc
     1501 tgccgattat gctcttttct gcgtgggcgg tggcattggg gcttacccaa gcggtctgca
     1561 aaatgctgtt ccacacccaa accaacacgg ggctatggac aaaactctgc ctgattttaa
     1621 ccgcactttc gcttgtcgga ctagcattca gtagtccaca agccacacaa gattttgccc
     1681 agctcttcaa tggctcaatc accgcttacc ttgtcggcat tttctggcta attgccctca
     1741 tttgcaattt ttctgccaaa aaccaccgct tgcaatggct cggcgtgctt gccctcatcg
     1801 ctttcggctg gctactccgc tgggtactgg tgatccaagt ccaaaccatt gccaaaacca
     1861 atgccttgca aaatccatat catttcgatt ggacagccgt cgatggtggt ttggggattg
     1921 tctcgatttt tgggctaacg gtgctagtaa gtatcgtggt atggcaactg attttaataa
     1981 tttgtggggg aagaaaaggt gaacaataaa cgcagaaatc tgttgaaagg cgggctggca
     2041 cttggtgggg cggcggcgtt tggggccggg tattcgccga aggtgtcgga gattgccaaa
     2101 ggggtgatgc acggcacatc gggcgagaaa acccgtgatg caattcacgg taattcgctc
     2161 acgccggagt atcaggtaaa agacggcaaa ctgctcagca atcctgagca aattgtgtgc
     2221 aatacacagt gtatgggctg ttggacgcag tgtggcattc gggcgagggt ggatttgaac
     2281 accaaccaag tgattcgcat ttcgggcaat ccgtatcacc cgctttcctc cgacaaaacc
     2341 ctgccgttcg gtatgccgat tgccaaggcg gaactgttgc tggcgggcga aagtggcatt
     2401 gaaaatcgct ccaccgcctg tgctagaggg gcggcgttct tggagggggt gaacagcccg
     2461 tatcggatta cccaaccgtt gaaacgggtc ggaaaacggg gcgaaggcaa gtggcagacc
     2521 atcagctttg agcagttggt ggaggaaatc atcaatggtg gcaacctatt tggtgaaggc
     2581 gaagtggagg gcttgaagac gatccgcaat ctggtcgatc cgattaaccc gaaacagcct
     2641 gatttgggaa tgaaagcaaa ccagctttta gttacttttg ccggccccga aggtcgtcag
     2701 ccgattttgc aacgctttgc ccaaaagagc ttcggcacga tcaacttttc caaccacggg
     2761 gcttattgtg gcgctgcgta ccgtgccggt tcgggtgcct ttatgaaaga ttttgacaac
     2821 caacaacacg ccaagccgga ctgggatcac gctgaattta ttctgtttat cggcacgtct
     2881 ccggcacagt cgggcaatcc gtttaagcgt caggctcgcc agcttgccaa acgccgccct
     2941 gatgacaatt tcagctatgt ggtggtttcc cctcgtttgg agatgacctc ctccctcgcc
     3001 acccaaaaca accattgggt gccgattaag cccgagggcg atttggcgtt ggtaatggga
     3061 atgttgcgtt ggattatcga aaacgagcgt ttcaatgctg actacctctc ccgcccaagt
     3121 ctggcagcga tggaaaatgc cggccacgtc agctatgcca atgcgtcaca tctgattatt
     3181 tgcgaaccga accacccgaa attcgggcag gcgttgcgga ttagcgatct gcaagatgtg
     3241 caactcgacc ccgaagagaa agtggaagaa aacctgattg tggtaaaaga tcaggcaagc
     3301 ggcgaactca tttcagcaaa agattgctta caagcggtca tttttgtcga acaaattgca
     3361 aaactgcttg atggctcgga agtgttggtg aaatcctcaa tgcagttgct gaaagaggcg
     3421 acacaagaga aaacccttgc cgaatatagc gacatttgtg gcgtgccggt gcaaaccatt
     3481 gaaaacttag cgaaaaaatt cacttcgcac ggtcataaag ccaatgccat cactcacggc
     3541 ggcacgatgc acagcaccgg tttttatacc agttgggcga ttttgttgct gaatgcaatg
     3601 gtcggtaacc tcaacaaaaa aggcggaatg gcagtagccg caggcaaatt caaagatttc
     3661 ggggacgggc ctcgctacaa tttagcgaac ttcccgaatg aagtgaagcc aaaaggcacg
     3721 aaccttgccc gcagtaaaaa ggcgtaccaa aattcaagcg aatttaagca gaaagtgacg
     3781 aagggcgaaa atccgtatcc ggcacaaggg gcgtggtatc cgttcaccgg cgggcaaact
     3841 accgagatgt ttacctcaat ggtgcagggc tatccatact ttgcgaaagc gtggatcaac
     3901 catatggcga accccgttta cggcttaacc gctatgaacc cgattgcgtt ggagaaatta
     3961 aaagatccga aaattctacc gctctttgtc tctattgatg cttttatgaa cgaaaccact
     4021 gcactgacgg actacatcgt gccggacacc cacaactttg agagctgggg cttttcaacg
     4081 gcgtggggcg gtgtgcctca acgcaccagc accgcacgcc acccgattgt gccgtccaaa
     4141 aacgccaaaa ctgccgaagg cgacaccatc tgtatggaga atttcgtgat tgcggtggcg
     4201 aaaaaaatgg gcttgccggg ctttggcgaa aatgcgattg ccgatttaga aggcaaccgc
     4261 tatccgctcc accgtaccga agattacttc ctacgggcgg cagcgaacat tgcctttgcc
     4321 ggtgagaaac ctgcaccgga cgccagcgag caagatctgc tgctcaccgg cgtgcaacgg
     4381 attatgccga aaattgaaca aacgctaaaa gccgaagagc gattaaaagt ggcgaatgtg
     4441 tattgcaaag gcggacgttt tgccccacac gaaagtgcgt ggaaagagga gaatatggag
     4501 ttccagtgga aaaactgcct gcaaatttgg aacgaaaacg tgtataaagc ccagcaccac
     4561 agcaacggtg aacactattg gggctgccct cgttatatgt cgccacgttt tgcggacggc
     4621 tccacgctgg agcagcacta tccgcagtcg gaatggggct ttaaactgat ttcgtttaaa
     4681 tcgaacatta tgagcagtat taccgcccca ctgttgcgtc tgctctcgat caagcctgag
     4741 ggcttagtgg cgatgaaccg tttagatgcg gaagaaaaag gcattaaaca cggcgatgtg
     4801 gtgaaactca gcaccccaac cgccggcttg acggtgcaga ttgtggtgtt ggacggtatc
     4861 gcccgaggca caatcggcat cgaacacggc tacggtcata aacagattgg ggcaagttcc
     4921 tacaccattg acggcgtgga aattccagcc aacccgatga tcgccaaagg gattaacctc
     4981 aacgacctcg gcattatcga ccacaccaaa gcggtcaaat ccccgtgggt agattgggtc
     5041 tgtggctcag cggtgagaaa tggcattccg gcgaaggtag agaaattagc ctagccgaac
     5101 aagcggttgg ttttgcagaa aaatgtgcaa aatcgaccgc ttttattgat gtaagggaca
     5161 ctgttcctta tgcaaaccag cattgcagag cgtaaaaaat catactctgc attttaattt
     5221 attttggaga acctatgact tttgtcaaaa aaacagcact gagcctgatt gcaacggcac
     5281 ttttcgctac atcaagtttt gccgctgagc agagccaata ccaacatatt cgtaatgcaa
     5341 cggcgaaaat tgaatatgcc ggcaagacgt ttttggttga tccgtattta gcggagaaag
     5401 atcgctactt accgccgaat gcgaaaaaac gtaatccgac cttggatatg actgtgagca
     5461 ttgatgaagt gttaaatggc gtggatgcgg tgatcgtaac tcacactcac cacgctcact
     5521 gggacgatgt agcacaacaa cgtgtgccga aaaatctgcc gatttttgtg cagaatgccg
     5581 gtgatgcccg tttaatccgt tcacaaggct ttaccgatgt gcgtgtggtc ggcaaagata
     5641 ccccgtttga aggcgtaacc ttaacccgaa ctcgtggcca gcacggcacc gatgcgatgt
     5701 atgcgaaccc gaaaattgcc gaaatggtcg gcgatgcgat ggacgtagtg atgaaagcgg
     5761 aaggcaaacc gaccgtctat ttggtgggag ataccatttg gaaccacgaa atcgaacatt
     5821 cgttcaacac ttacaaacca gactttgtgg tgctgaatgc cggctgtgcc aaactaccaa
     5881 actttgaagg cagcccgatt atgtgtaccg aagatgtttc caaagcacac aaaatcgctc
     5941 cacaagcgaa aatcttaact gtgcatatgg acgccctgcc acaaacctca atcagcagtg
     6001 atcagatgcg tgattatgta caaaaagagg gattaaatcg tgtaactgtg ccgaaaaacg
     6061 gtgatgtgat taagttctag tagctaaact aacattacca aaatggttta agtagcaata
     6121 aatagcgaag cgggttaagc catcaagcct taacccactt ttaccaacca tagtatatag
     6181 ttttagatat aattatcttc tgtatttacc tgccattgct tggtaaaccg cattctcgtt
     6241 ggtcaaaatc gcatattttg catttaaaat cgccaactga gaattaagct cggtattcag
     6301 tgcggtaatc cactcacgga actccgacac cccttgttca taacggtttt tatagtatga
     6361 actgatacgt ttatttttcg cattggtatc ttgaaggatt tttaacgagc ttctcgactg
     6421 cgtataactg taataataac ggtcaatttc atttaaggct gaggtaattt tttgctcata
     6481 gtttaaacgg gcagtcacat aatccgtttc agaaattttt acgttatttt taacccgatt
     6541 ccaatctaag aacggtaaat ccaatttaat taagccgtta cccacagggt tatctgccac
     6601 attgccgata ttggttgcac taccggtaat agaaccaccg atggttaagg ttgggaacca
     6661 gcttttctcg gttgcggtta aggttttaaa tgcactttgt aagcgttgta acgagcctac
     6721 tacatcaggg cgattagcaa tggctgacac tggcacattt aaatctaccc cttgcaattt
     6781 cacgctcata atattcgggt aacgaatgtt aagcggagta tttggctgaa catttaacaa
     6841 attgcgtaat gtttgctcgg caattttttg gttggttttc agatcaatta cactatttct
     6901 ggcgtttaag accgcttgtt gagcctgatc aaccgctaaa ctatcaatcg caccggcatt
     6961 cattttgtta gttaaaatac ggctgatttg ctcataatgg cgaatgctct cttcggtaat
     7021 acgaatcgca tcacgatagt aagctaactg gtagtaagta gtcacaaccg cattaataat
     7081 agataaacgg gtagctttta agtcttcaat ggtggcttta tgcgtccatt ctgccgcact
     7141 ggcggtatcg gctaaacgtc gccataaatc cacagtataa cttaaattaa atgcagcttg
     7201 agctgcggtt gttgaaacac cggtcgaaac attatttgaa gaagccgaac ctaaaccttt
     7261 agccgcagaa acagaacctg aaccactaaa ggttggcact aaatttgccc ctactaaatt
     7321 agcgttatac aaggctttat tgactgcaat tccgcttttg gctaaatcaa ggttattttt
     7381 gatggctaaa tcaaccacac ggtttaattc cgcatccgta tagccttgcc accattgctc
     7441 gttaatttga tattgtttgg taatttcatc atagctttga taggtttgct gtgcttgttg
     7501 caaagtcccg tcattgctca tattccctgt ggtacaagcc gttacggtta gagccaacgc
     7561 cacagaaaat gctaattttg aaagtttcat tacatttcct tttgaaaata aagaggggaa
     7621 aatgggcgta ttctacccaa aaacagattg cctttccagt taaatgaaca actgttcatt
     7681 taattattta acaaatcagt aatcaaatgc tgatgacgaa tttgttgcga acctaagctc
     7741 aaacgctcgg caattaaaat cgcttttagc tctgccaatg cctgttggaa atcatcatta
     7801 ataatcacat aatcgaactc attgtaatgg ttcatttccg acaccgcctc tgccatacgt
     7861 ttggcaatgg tttcagcgga atcttgccca cgcccgatta aacgtttttc caattctgtt
     7921 tttgacggtg gcaaaataaa aacggttttc acattcggca ctttttcccg aatttgtcta
     7981 gccccttgcc agtcaatatc caaaaagaca tcaatgccct gctctaggct acgttcaatc
     8041 atcggcaatg atgtgccgta ataattgcca aacacctccg cccattctaa aaaataccct
     8101 tgctcaatca gctgcttaaa ctcatcgtgc gaagtgaaat aataatgcac gccgtcctgt
     8161 tctgccggac gaggctgacg ggtagtgtgt gaaatcgaaa gctgtacttc cgaacggggt
     8221 aaatccgcca acaaggcgtt aattaaggaa gattttcctg ctccacttgg ggcagaaata
     8281 atataaagat tgcctaaact cattatttgt atatacctat ttgattttgg gaatagggcg
     8341 aatgatgatt cgcccctaca tttcatagga ataaataaaa aataatggac aactttcatt
     8401 gtccattaca caaaattatc tacgtgccaa aggctgaacg aaggtaatgc ctaaatccca
     8461 aggctgctca atccacgttt cttgtggaat atcaatcaca taatcatcaa ccaacggtgc
     8521 accgtgcggt ttcgcaaaca cggttacaaa acgagctttc gggtacattc tgcgaatttc
     8581 ttttgccgtg ttgccggtat ccactaaatc atctaccaca ataaagcctt cgccatcggt
     8641 atcggctgcg tgtaacacat ttaactcacc ttggctatcg tgatcgtagc tggcaatgca
     8701 cactgtttct acgtggcgaa tacccaactc acgtgctaaa acggctgccg gaaacaagcc
     8761 gccacggctc accgcaataa tccctttcca ctgtgtggca ggtaataaat tttctgccaa
     8821 cttgcgagcg tgcatatgga acatatccca cgtcacgaca tatttttcat ttgtgtattt
     8881 ttcactcata gaaacaacct aatataaaat tgggggctac cccgatataa aaaattgcac
     8941 taggataacc cgaaaatgct tttttcgcca tcatttcatt tatttttttg tgccggcagc
     9001 tacgcaatcg tatgcttagc acaaaaaacc aacactaaaa tagcttttct aaagtgaaaa
     9061 agacttcctt gttttggcga gagtaaaaat cagcaatatt gctgtcaatt ttttgataac
     9121 ggaaattcag cttcggcaca atgccctgcc aatgccaagt gcggttccaa accgctaaat
     9181 tggcttgata ttccgtgtct aaccgcttat agccaaacaa atagtgcttg tcggcaaaat
     9241 atcgcttgcc aaagccgata cttgcctgcg ttgcccagcg ttcgccttga aaaaccgccc
     9301 ccactcgagc cagccactta ttcgagcttt gtgctttttc ccgtgcaatt tcacgcacgc
     9361 cgtctaaatt tgcaaaaaac cgcccatttt tgaccgcttg ccactgcaca ccgccactga
     9421 gttggtgctg atagctgtta taacggcttg cggttagctc ctcaaaatac cgtttttgca
     9481 aatgggtaaa actccccgac accaaccatt gcgaattaag gtaacgcaaa ccgttgagtg
     9541 ttgccccaaa ttgaacatta taacggctac tccccagcca attttgctca ataaaaggcg
     9601 aaaaagtcag ccgataagcg acggtgcggt agctataatt cggcgaaacg gatacggatt
     9661 gctcacggta atctcggtta tcccaatagt aaatcccgct ataattgagc gttaaaccga
     9721 caaaatgatt gcctttcacg ttgtgcaatt tactcaaccc taagccataa cgcacgcctt
     9781 ttgctgattg tggcaaactg tctgcatttt tgcggaaaat ccgcccattt acattcacaa
     9841 tcggcgaaga tgaggcatta ttcacgttat ctgtttgggt atattgcaga taaaaatcgg
     9901 gctgccacgc ctcattttca gcaatttgga cttgagtatt gcgggcaatt ttagcaagtt
     9961 ccggcggtaa atgggcttga atagccttaa attcctgctc tgccgcacga aattgctgat
    10021 cttctgccaa aatcaacgcc aaatcaaaac gagggtaaac caaactcggg ggcttagcta
    10081 acagttggcg atagtgaaaa actgcggttt gatgatcttt tttcgcatag gcaagtgcgg
    10141 cttgcgcata gtgccacaac aacgggtcaa aattttcagt ttgcggataa cggcttaaaa
    10201 gcggttcaag cgttgcccaa tcttggctga gaatcgcttg gttaatttgc tgttcaacat
    10261 tctgattgac tgaatacgca ggctgataag cggtcggttt ttgtgaattt tttacaacat
    10321 tttgctcaaa tttaaccgct tgcggctgac gctcaaagga aatcgggtca gtgttgtgtt
    10381 caatcacctt ttctgcccat acaacggggc tgaacagcga aaataaaagg ggatattttt
    10441 tcataaagat aaagaaaatt ggtaggggcg gacctatatg ttcgcctcta cgggatttat
    10501 cactctctcc ccttgtggga gagggttatt agaagctgta aattgaaatc taaactattg
    10561 tgtcttatgg tatctacaag caatatcaag acaaatgccg tttaattttt cgttaaacgg
    10621 cattgaacgt tagaaaaggt tatttttgga tatcgccacg agtgccggca agtccaaaat
    10681 tactcacttc cccagtaaga tttgcttttg tctgaatttc accggcaatt tcctctgcat
    10741 ttttaccgaa aaattcgact gtatattttc cagaaaggtt taaaggatta ttgccatgta
    10801 ctgttgaaga aatctgagta ttattaatcg tcccctcttc taatacgatt aagtttccaa
    10861 attctccctc aactctacct gaaccggtac gcttagagaa attaacatta taggttagat
    10921 caccaccatt aaaaccttta gctccatcgt ggttaaatgc ttttcctgaa tatgttgcag
    10981 taccctctgt tggtaatttc tctgttttca atcctttaac tgtaactgtt ccatcaactt
    11041 tatcaatata attaccgttt tcccagccat ttctttgaac tgcttgtgct gttactacag
    11101 aatatggctg attatataaa taactctgtg ttctgactgt gactttaccc tcaatagttt
    11161 ctttactatt accaagtttt aagccgtaat ttgtatcatc aatacctttt gctttaataa
    11221 tagctatagg atctacttct ttttctgctg ctaaacgctc cgcttccgct ttctctttcg
    11281 ccgctttttc cgccgctaaa cgttctgctt cggctttttc tttcgctgct ttttccgccg
    11341 ctaaacgctc cgcttccgct ttctctttcg ccgctttttc tgccgctaaa cgttctgctt
    11401 ccgctttctc tttcgccgct ttttccgccg ctaaacgttc tgcttcggct ttttctttcg
    11461 ctgctttttc cgccgctaaa cgttccgctt cggctttttc tttcgctgct ttttccgccg
    11521 ctaaacgttc tgcttccgct ttctctttag ctgctttttc tgctgctaaa cgttctgctt
    11581 ctgctttctc tttcgctgcc ttgtcttttg ctgctttttc tgctgccgct ttatcggttt
    11641 gttgctgagt agtttgcggt ttagtattcg gttttgccac attgtttgag ctactgcccg
    11701 aaccaccact actacacgcc gttaaaccca ataaaatcaa actggctaaa gttacttttt
    11761 tgatagacat aaaatgttcc ttttgtaaga taaacaaaaa tagatgtgta taaaacacac
    11821 gcatattgta caaaaaaaaa acaactcgtc atcaagtact ttttaagatt tttttgttaa
    11881 atttctccta aatattctac aatgtcagag gtctatatca gcttgaatac aagcggttat
    11941 tttttcttaa aattttacaa aaggggctta ttatgtctga aatctcaacg ctcgcaccgc
    12001 agctactttg gcagtggttt gataaagcct gtgctattcc gcacccgtct taccacgaaa
    12061 atgaactggc tgaatttatt gtgaattggg caaaatcgaa aaatttcttt gttgaacgtg
    12121 atgaagtggg taatgtgtta atccgtaaac cggcaactaa aggtatggaa aaccgccaaa
    12181 ctgtcgctct gcaagcccat cttgatatgg tgccacaagc caataccggc acggttcacg
    12241 atttcactaa agatccgatt cagccttata ttgacggcga atgggtaaaa gccaaaggca
    12301 caacattggg ggcagataac ggcattggat tggcttcgtg cttggcggtg ttggatagcg
    12361 atgatttagc ccacccaacc attgaagtgt tgctcacaat gaccgaagaa gccggtatgg
    12421 aaggggcgat tggtttacgc ccgaactggc taaccgccga tattatgatt aacaccgaca
    12481 ccgaagaaaa cggggaaatt tacatcggct gtgcaggtgg ggaaaatgtg aatgtgacac
    12541 tgccaatttt cttggtgcct catcagcaag atagtgcagt aaccgttacg cttaaaggcc
    12601 taaacggcgg gcattcgggc tgtgatattc acaccacccg agcgaatgcg attaaagtgt
    12661 tcgcccgcat tttggaacaa gcccacaacg aagtggaatt ccaaattgca gaaattcaag
    12721 gcggttcggt gcggaatgcc attccacgag aagcccacgc aaccttggtt ttctctgcaa
    12781 aaaatcagca aaaattaacc gcttgtttaa gccaaaccgc cgaacaagta aaagcagaac
    12841 tcaaaattgc cgagccaaac ttccaatttt taattgaaga agctcaactg cctgagcaag
    12901 cctttgacct tgccagcact gctcgagtaa tccatttctt aaatgtgatg ccaaatggtg
    12961 tggtgcgtaa tagcgatgtg gtggaaaatg tggtggaaac ctcactcagt attggtgtgc
    13021 ttaacaccga agcgagtgag gtgaaagcga ccattcttgc ccgctcatta attgagggcg
    13081 gaaaagagga aattcgcagt aaaatccgct cattaaccgc attagtggga gcagaagtgg
    13141 aattttcggg caactacccg ggctgggagc caaacccaaa ttccaaaatc acgccattaa
    13201 ccaaagcgat ttatgatgaa attttaggtt atgaatctca aattaaagtg attcacgcag
    13261 ggctagaatg tggcttaatc aacaaaatct acccgaatat ggattttgtc tcgattggac
    13321 caactattct caacgcccat tctccggacg aaaaagtgca tattccggca gtggagattt
    13381 attggaaact cctaaccaaa ctacttgaac aagcacctga gaaataaatt taatcaaaaa
    13441 aattatataa tcttgccccg taattaattg aataaaacct caattaatta cggggtagct
    13501 cttattttct ctgttttaaa aaatgtaccg tgaaagcagc aattaataag acgactgctg
    13561 cacctaaata aagctcgttc tttttccagc cggcgtctaa taaaatacct gcaagagttg
    13621 gggaaacaat tgagcctaaa cgccctacac cgatggcggt gcctacgccc gtgctgcgga
    13681 aatccggtgc gtatagggtt gggttaatgg tataaagccc ggtaatgcag ccgttaatta
    13741 atccgccaac caaaatggct agtgttatcg caagccctaa attgccggca aacacaaagg
    13801 caaccacagc agatgcagcc agaatactga atgcctgcaa aatatgtttt gccgcccagc
    13861 gactgactaa aaagccgaaa attaacgagc cgattgtacc accaactgaa attgccattc
    13921 ccacagtttg gctttgggtt ttaggcattc cggcttcttc taatagtgcc ggagtccaag
    13981 agctgataaa gtaaaatgag gctgaaattg caataaaggc aagccaaatt agcaacgtat
    14041 tgcggcgata ttcaggctcg aataaacggg taattgccac tttgctttta ctttttgcct
    14101 caatttcagg aagttcccag ccgtttccta agcctaattt aacggcaatt tgatttaaac
    14161 gagctttagc attcaccggg tgttttgaaa ttaaaaattc caccgattcg ggcaatacaa
    14221 agaataataa aacgaatacc gcaccggtta aaatcgaccc aacgaggaaa accgcacgcc
    14281 agccatattc tccttgcaat aacaccgcag acattccgcc cagcattgca ccaatcccaa
    14341 agcctgaggc ataaattgca atcgctaagc cacgccattt ttggctggag tattcactca
    14401 ctagcacatt agtgccggtt aaaatggcgg ctacgcctaa accggttaaa acacgccata
    14461 caccaatttc aaacgcagaa gtagcgaaat aagttaggag cattcctaac gtggctaagc
    14521 ctgtgccgat taaaagcaac gggcggcgac caaatttatc agcaagtggt gaaagaaaga
    14581 gtgagcctat cgccataccg aataaaccgg cactcattaa gctgccgatg gctgcaccgt
    14641 ctaactgcaa ttcttctcgg atgcttttgg cggtaaaggc gagtgccatt aaatcaaagc
    14701 cgtccagcat attaagtaac accgtaagcc caaccactaa ccattggaat gaagtcattt
    14761 tgttatgagc aattttttga cgaatatcca taagctccct ctgcatcaaa attgataaaa
    14821 ttttatttag agtgaactat agaaagttga ttaaatataa tcaaactcaa aatttacata
    14881 aaatttatga aagattttca tatttaatca aaggagggaa tttatagcag gaatggcgag
    14941 ggagaaaata gatagggcga ataattattc acccctagat tgaataggag aaagctattt
    15001 cttctccgct tgctcagccg ctcttttgcg tctgatttct ttcggatcag caattaacgg
    15061 gcgatagatc tcaattctgt cgccttcttc caccgtttca ttcagttttg ccgggcggct
    15121 aaaaataccg actttgtttt cacgcaagtc aatttcggta tatttttcca acacgccgga
    15181 gagcaaaata atagtttgaa tggtcgcgtt ttgcggtact tgcagttttt tcagaaaata
    15241 tttatcaggg taagcataaa gcacctcaac attaattttc tgttcttcac ttttttgttc
    15301 ttctggattt tgacgttgtt cagacaccat acacctcttt tgcacgttgt ttgaaagcat
    15361 ttaccatttt taaagttaat tcgttgaaca ccttgccgaa caccattgct acaattgggc
    15421 tggaaaattc aaaatttaat tgcagggcaa ttttgcaact ttgctcatca aagggggtga
    15481 atgtccaagc tccgtgtaaa tggcggaaag gcccttcaat taatgccatc tcgattttat
    15541 agggcggcgt cattttattg tgggtactga aagtttggct aatgcctaat ttctgaatgt
    15601 gtaactctgc ctttaattca ttttcgccgg tgctaagggt tttggtactc acgcagccgg
    15661 ataaaaattc ggggtatttt tcatactgat taacaagttg atacatttgc tcggcactat
    15721 aaggcacgag ggcagattga ttaacgattg gcataatgtc tcttttggga attaacaagc
    15781 ggtcatattt tagcgtaaat ttgcaagcct ataaagaaac gcttttgatt tgaagataga
    15841 cctcttgctc cggttgaatc gctaattcat taaaagacca aaggctgatc gtactccaaa
    15901 tttgctgatt ttcgacttga attgccagat caacccgatc taaatgcgtt tctattttcc
    15961 gaacattgcc ttttaacaca ttacgaattg agctttgagt cggtttttgt aagctgattg
    16021 aaacctcttt gctggcaata gttaccctta gcttctcgcc tatttgatgg cgggggagtt
    16081 ggttcaccca taatcgctgt gcgttcaagc tcaagccaag catattatag tggggaagtg
    16141 tttcttccac cggtaattca agcaggctaa ttcgctgatg atttggctgc caagcggaaa
    16201 atgccgagct gtgccaaacc gctgaaaggg tatcaaatgc ggttactttg ccgttttcca
    16261 acagcacaag atgatctgcc aatcgaacta cttcctctaa actgtggctg acatacagaa
    16321 tcggtatttc aagccgagcg gataatttat ctaaatagtc cagtagctct tgcttacgag
    16381 ggagatcgag ggcagataat ggttcgtcca tcagcaaaat attcggtgag cttaataacg
    16441 cccgcccaat cgccacacgt tgtttttcgc caccggaaag gcttgccgga tagcgattaa
    16501 gcaaaggggc aatgcctaac agttccacaa tttgcaaaaa atccgcagaa tccgaccgct
    16561 tgtagccata ttttaagttt ttttccaccg aataatgagg gaaaaggcga tgctcttgaa
    16621 acacatagcc aatgttgcgt ttttcaggtg gtaaattgat gccgttttgg ctgtcaaaca
    16681 gcacctgacc gtttaattcg ataaagccgg cttgagcggt tgaaagcccc gcaattaaat
    16741 taatcaggct ggatttaccg gcaccggaac gcccgaaaat agcggtgatt cctttattcg
    16801 gcaaatcgag attgacattg agcgtcagat tgccgagttg ctgaagaagt ttaagtttga
    16861 gcatagcgtt tttgcctttc tgccagccat tccgaacaga acagggcaat gagtgaaatc
    16921 agaatcgcca ccaagcacaa acgggcggca gccatttcgc catcgggggt ttcaataaag
    16981 gtgaaaaggg cggcaggaat ggtttgggtt tggttgggaa tgttggagac aaaggtgatg
    17041 gttgccccaa actcccccaa agagcgggca aaacccagca cgccaccggc aataatgcct
    17101 gaaaaagaga gcggtaaagt gagcgtgaaa aacactttta acggtattgc tccaagcgtt
    17161 ctggcggctt gctccaattt aggatcaact gcctcaaagg ctaaacggat agaacgcacc
    17221 attagcggaa acgccatcac cattgaggca agcactgctc ctttccacga aaaactcaag
    17281 ctgaaatgaa accattccca caaatattgc ccgattaccc ctcgtttcgc catcgaaata
    17341 agcaataaat agccaatgac aaccggcggt aacaccagag ggagatgcat aataccattg
    17401 agcagatttt ttccccaaaa ctgtttgcgg gcaagcagcc acgccaccca cacggcaagc
    17461 ggtaatgcaa gcactacagc aatggaggca acttttaggc ttagaacaat cgcattgagt
    17521 tcttgtgggg tgaaattaag cataatctaa aactatttgg ttataagctt accctcgccc
    17581 cttgtgggag agggatagat ttttcttcgt tcagaagaaa aatcagggag agggggaata
    17641 ttttaaacgc tatttgccct ctctctgaaa aatatgtctg aacgacatat ttttctgtct
    17701 ctctcccaca aggggagaga gtaaatttaa tttacaggat aaaacccagc tgcctcaaat
    17761 ttcgcttttg ctttcggggt tttgagaaaa tctaaaaatg tggcggcaga ggctttttca
    17821 ctgactgccg cagcagggta aacaatttcg ccgtagcttt ccggtgggaa aacggcaacc
    17881 actttgactt tattcgacac tttagcatcg gtggaataga caatcccaag tggggattcg
    17941 cctcgctcga caaaagagag ggcatctcgt acattttttg ccctcgctaa acgaggttca
    18001 acactttccc ataattggta atgactaagg gcttttttgg catatttgcc ggcaggaacg
    18061 tggtttgggt ctcccaccga taaatagctg tttgctaatt ctttggcaaa atcgaccgct
    18121 tgtatcgctt ttggctcaat gttactttcg gtaggtgcaa ttaatactaa ctcgtttctg
    18181 actaaattgc ggcgatgttt aactttctca ggttgttttt cggcaacata atccatccac
    18241 ttttggtcgg cggaaataaa aatatctgcc ggtgcatctt gctcaatctg ctttgctaat
    18301 accgatgatg aagcgaagga gaacgtgatt ttatcctgtg gataggcttt ttcaaaatcc
    18361 tgctgaatat cttgtaacac atttgtcatt gaagcggcag caaacacggt aatctcttgt
    18421 gaaaaggctg tgcctgataa cgttaatgcc attgtaattg ctagtttttt taatgctttc
    18481 attttcataa cctcacaatt agttaagaac gcagcccaac ccctcgttga attaaggaat
    18541 aggcaataat atacaagccg atgcaaagtg aagtaagcac tgcaaaagta taacctaacg
    18601 gcacatcgct gataccgaga aagccatatc ttaagccgtt aatcatataa acgatcgggt
    18661 tgaatttaga caccgtttgc caaaattcag gcagtaatga aatagagtaa aatacgccgc
    18721 ctaagtaagt taagggagta agcacaaagg ttggaatcag ccccacatca tcaaaggagc
    18781 gggcgaacac cgcattaatt acgccaccta acgcaaaggc aatgattgtc agcaacaggg
    18841 ttaagacgat aatcgcaacc gagtagattt caaacgagac aaacaccatt gccacgcaca
    18901 ttactaaggt tgcggctaaa cctgcacgca aaatactgcc ggtgacataa ccccaaataa
    18961 tattatgggt agagagtggc gaaatcagca tctcttcaaa atctcgggta aatttactca
    19021 agaaaaatga agaggcggta ttcacataag aggcggtgat ggcggtcatc ataattaagc
    19081 cgggtgcaat aaattgggtg taacccacgc cattcatttc cccgattcgc ccaccaatca
    19141 gcgtgccgaa gattaaaaaa tagagcgtgg tagtaatcac cggtggcact aaagtttgtc
    19201 gccaaatccg caaaatgcgg cggcactctt tgtaagctaa ggtataaaat ccgattaaat
    19261 tcattttact gagccatatt gataaatagt tcttccaagc ggtttgattt gtttcgcata
    19321 cttagcacct cagccccttg ggcggaaatt tgcgaaaaca gattggttag cccttgctgg
    19381 cgttttacat tcacttcaag ggtgttttca tcaagccatt tatgcgggta attttgaatc
    19441 acaagcggtt gattttgctc agaattttgc aaatctaaca caaatacctc actttccaat
    19501 ttggcaagca aggctttcat tgaggtgttt tcaattaatt cgccgtgttg aataatgccg
    19561 atgtgacggc atagggtttc cgcctcttct aaatagtggg tagtgagaat aatagttgtg
    19621 ccttgattgt ttagatcccg caagaaatcc cacaatgagc ggcggagttc aatatccacc
    19681 cctgcggtag gttcatctaa aatcagtagt tttggattgt gcattaaggc tcgggcaatc
    19741 atcacccgac gtttcattcc accagaaagc tcacgggtga ggtgggtgcg tttttcccat
    19801 aggtcgagca atttcagcca cttttcggca cgtactaagg cttcactgcg aggaatgccg
    19861 taaaacccgg cttgttgcac tagcacatca atcacttttt caaactgatt gaaattaaac
    19921 tcttgtggca ctaagccgat ttgatgcttt aattcgactt tttgggtgtc taaatcatag
    19981 ccaaacactt tcacgctgcc gctggtttta tttaccagtg agctgataat gccaatagta
    20041 gtagatttac ctgccccgtt atgcccaagt agggcataga aatcaccttg ctcgaccaca
    20101 agattaatgc ctttaacggc ttttacacct gttgggtagg tttttaccaa atcaattaat
    20161 tctaatgctt tcatttgttt tactcagttt ccgccaccgc tcgaatggtc gattcatcaa
    20221 tttgtgcatc aaaagcgttg cgaagtaaca ttaatttttc atcgttaagc agagctgatt
    20281 ttgctgcctc agttaattgt ttgaaaatgg ctcgccgaat ttcaagcgga gtttgctgtt
    20341 cgctattgtc accaatggtc aattcgtatg aatagccttt ttcggcaagg gcagcttcta
    20401 aatttttacg tgattccgga ttatctaagt gagccatatt cggctttaac accaatgcga
    20461 ggtgctgttc attttgttcc gctaaatagc tatttaaggc gatctgacgg gaaaatccgc
    20521 ctaaattgag tgagttggcg atgtcacacc atttatcctg ctgacaagat aaatgaatgg
    20581 tttttgccac taattcaggg gtgcgttctt gcaaaatcgc ttgtttaata tcagaaggtt
    20641 tggcactgtc tgcttgattt tctaattcag gattgagcca cgtccagcga taatcttcat
    20701 cttccacact tttaggggcg gattcggttt tctttgaaac ttgcagtacg gctttgtgct
    20761 gattttccgc caagctgata aaccgctctt gcaatgaagc ggtctgattt gccgattttt
    20821 ttgcaataac ttgaggggct tttagctcag gctttttttt ttcggtttga gctaaatttg
    20881 cctgctgtgc ttgtagcttt ttgcgggctt cgagggctgc cattgccggt gaaagagtcg
    20941 caggagcaga cggcgaaata gcggtatcta ctgcctgtgg cggttgagca atgctttcaa
    21001 ccgctgtggt agtattggca acagtaaccg ttttttgctg attttgctca aaattttctc
    21061 ttagctggct aatttgattt tgtggttttg ctgtcgtaac cgctgaaacc gaatggtttg
    21121 gttgtgtaac cttttccact gcaactgttg ctattttttc tgcctgttta gggtggaaag
    21181 ccaacgcacg caaaatcgcc atttcaacac cggcacgttg ctccggtgca aaaggcaact
    21241 cttttttact gttgaggatt aattgataga aaaattgcac gtcttcgggc ggaatttgtt
    21301 ttgccagaaa atgcactaat gtttcttcgg ttgtaggtgt tttcactaat tgcaacattg
    21361 caattttgtg taacacttct gccacatcgc ttaataattg ttgccaatca acgcctttcg
    21421 ctgcaatttg ctcgatgatc gccattgctt tttcaccgtc ggcttgggcg gttgcttgca
    21481 ctaattctag tggttgatta tcgtcaatca accctagcat ttggctgact atcgcaagcg
    21541 taatattggc attactcatt gcaatggctt gatcggtgag gctgagagag tctcggatac
    21601 taccttgtgc tgcttttgcc aacttttcta gtgccaaagg ctcaaacgga atgttctctg
    21661 catttagaat aaattcaaga tgacgggcaa tttgcgattg ctctaatgcc cgcagatgaa
    21721 attgcataca gcgagagagg atcgtaatcg gcagtttttg cggatcagtg gtagccagca
    21781 agaatttgac atattcaggc ggctcttcaa gggtttttag taaggcgttg aaactatgac
    21841 gagagagcat atgcacttcg tcaattaaat aaaccttaaa acgcccaacg gtgggtttgt
    21901 attgaacgtt atcaagtagc tcacgggtgt cttccacttt agtacgagaa gcagcatcaa
    21961 tttcaatgag atcgataaaa tgcccttctt caatcgcttt acaattcgca cattcaccgc
    22021 aaggctcggc ggtgatcccg tttacacagt ttaagccttt ggcgaataaa cgggcaatag
    22081 aggttttacc cacaccacga gtgcctgaaa acaggtaagc gtggtgcagt ctatcttctc
    22141 ttaagctgtt ttcaagagca gataaaacgt gttgctgacc aactacttcc gagaagcgtt
    22201 gcggtctcca tttacgggct aaaacttgat aactcattga tttttctatt caatttggaa
    22261 atcacaagcg gtcatctttc cgaaaaattt tgcaaaattt tagcaaaaag ccaccgcttg
    22321 ttccccatta atgaccttca aagctgacta ggctaaagga tttaacccct tctttttcca
    22381 aacgctcttt accacctaac tcaggcagcc aaatcacaaa ggcagcgtgt tctacgttac
    22441 cacctaagcg acgaattagt tttgcagttg catcaatagt accaccggtt gcgagtaaat
    22501 cgtcgatgac caatacatta tcattagcac tcactgcgtc tttatgaatt tctagcgtgt
    22561 cttcaccata ttcaagcgtg taagattgcg aaatcacttc acgtggtaat tttttcggtt
    22621 tacgaaccaa cacaaaaggc acgcctaacg ctaatgcaac aggggcacca aaaataaacc
    22681 cgcgagattc tgtgccgaca attttagtaa tacctttgtc tttaaattct gcaacgatgg
    22741 catcaatggt ggcttggaat gcagttggca cttctaaaag tgaagtgata tcacggaaga
    22801 taatccctgc tttcggataa tctggaatgg atttgataga agatttaatt aaatctaatt
    22861 ggttcattgt ttgacctacg tgtaataaaa aaataactgg aaaactctat agaaaaacag
    22921 cagaaatttc aaggagaaat tgataacctt acgtttgata gtacagctta cttttcagtt
    22981 tccttttccc tatattatag ataaatatct tccctgtttc gtctggtttt aagcaaacgt
    23041 aaaatccaaa catactgctc ggggtttttc tccacaaaat attcaattac tcggttcatt
    23101 tctcgggcgg attgaattgg gtctcctgaa aattcaagcg gtggcaaaat ttccatttca
    23161 tattttgctt tttccgcatt ataaatcgag aacattggga tcacttccgc atttgccact
    23221 cttgccattt tatttaagcc cggcaaagtg gctttttcgg tggcaaaaaa gtcggcatac
    23281 acacttagct cttcgccgaa atcttcatcg ggcagaaaat aaccaacatc acctttacgc
    23341 acatcggcta aaaacgcttt aatcccattt tgacgagtgt gcattcgccc acctaaacgc
    23401 tcacgggtaa tattccacag ccagtccacc aacgcattac ggtgtggatt gtacattgat
    23461 gtcatcggat aaccgagcga gaacatcgcc acccccgcca tatcaatcga ccaagtatgt
    23521 ggcactaaca taatcacgtt tttaccgctc tctttggctt tcaccacatt ttcaaagccg
    23581 gtaaattcac accgtttttg taaataagag gccgggcgga ttgcagtttc gccaatcgcc
    23641 aacatggttt gtgctaccgt gataaacatt ttctcgacca cttcttcacg ctttttctca
    23701 ctccaatcgg gaaaacagta acgcaaattc actttagcac ggtggtagcc ttttttattt
    23761 ttctgcttga gatagcgata cgccatctta cccacaaaag cggataattt atccctaatt
    23821 cgcaccggca cataggctaa aatcactaat cctaatacac ctaaccatac gccccaatat
    23881 ttcgggtgta gaaaagacca taggaatttg tgcgtatagc cgtcttgggc ggtaattcgt
    23941 gtcattctat aattcctcat tatttgtcac tttctttgct tcgccaaaga aagtaaccaa
    24001 agaaaggcaa ccctacttca ccgctatctt cgcttggttt gaatttgctt aacgaaaaaa
    24061 ttccaaactc gccgcttcgc tgctcaaaca aggaattttt cctacaaatt caaagtcgtt
    24121 cgggcggttc agaagggaac ccggcagagc atcattttgc aaaattttcc aaaaaaatca
    24181 ccgcttgtat ggtcggatcg ggttcccttc tgagttgccc gaacgacttg aaatttttag
    24241 gaaaataact cgagcgagag cgaagcgaac atcagggtta tttttcgtta agaaaatttc
    24301 aaccaagtga ggatagcaac aaagcagggt gtgctttctt ttggttactt ttctttgcac
    24361 aagcaaagaa aagtgacata acacatcatc tcttatcaat aatattcttc accgccaata
    24421 tcaccccaag tccaataaaa gcccccggag gtaaaattgc cagtagtaaa cctgaatcaa
    24481 tatgcaatac atcaatgcgt agggcttttg cccaatcgcc aaggagtaga tctaagcctg
    24541 aaaacagcgt gccgttgcca ataatttcac gcaacgcgcc tagcaccaca aggctaaaag
    24601 tcattcccag ccccattgaa aagccgtcaa aagccgagaa tttgacctca ttttttgagg
    24661 cataggcttc tgctcgtcca atcacgatac agttggttac aatcagcgga ataaaaatcc
    24721 cgagcgattg gtaaacaggg aaagcaaaag cattcattaa aagctgtact gccgttacca
    24781 ccgttgcaat taccatcaca taaatcggaa tccgaatatc gtgtggagtg attttccgaa
    24841 acagggaaac cgtgatattg gtgcaaatta gcacaaacat tgttgccaag cctagtccaa
    24901 gtgcattggt tacgctgttt gaaaccgcca gtagtgggca aagccctaat aattgcacca
    24961 aagcaccatt atttttccac acaccatcag cgaaaatcgt tttccaaacg ctggattgtt
    25021 tcggggcaac gtcaatattc gtcacaggga tttgttgttc tctttccatt tctcccccta
    25081 tttgcacgct ttaaattgat taaacattgt cgggttttga gccagctctg tcaccaccca
    25141 tttcgcactt tggcggatat gattgaccac cgcacgaggt gtaatagttg caccggtaaa
    25201 ttggtcgaac ttgccgccat ctttttttac attccaatcc gcttcgtttt ctaaactaaa
    25261 aagttgattg gtaaaagata agatccaatc cgacactcgg gtctcaattt tatctcccag
    25321 ccccggagtt tctttatgct caagggtgcg aacgcctaaa atgttgccgt tcggctcaat
    25381 ccccatcaat agcacaatat cgccggaata gccgtccgga gccgttcctt gaactaggta
    25441 agcggtcaga ttttccgatt tttttgcaac ataaatacgg ttcaaataag gggcattttc
    25501 gagctgaacc agtttgcaag tcgccaacac atcattatca aaatggcttt tcggcacaac
    25561 ctcttccaat aatttgcgtt gttgcattgc ggttacatca tcaatcttgc ctttggtaag
    25621 caggtacaca ccggttgaaa cagcggtgca aagcaaggca ataaagccta aaattaaggc
    25681 gtaatttaag gtaatttttg aggtgttcat tatctgttat ccaattttgt gccgtataat
    25741 ctcggctgcg tataatggtc gattaacggt acgcaaatgt ttgccaataa cactgaaaat
    25801 gcaatcgcat cagggtaatt tccgtagtag cggattagat aaaccaataa tcccaccaag
    25861 ccaccgaaaa tcagttttcc tcttggggta atggaggctg ttaccgggtc ggtcgcaata
    25921 aaaaatgccc cgaacatcat cgcaccgcta aagaaatggc tcacaaaatg caagtgcgat
    25981 tgttggctaa acagctcggt gaatccacta aaaaaggcaa aagttgccaa cattgctacc
    26041 ggaatttgcc aatggataat ccgtttataa atcaacacca aaccgccgaa taagaacgca
    26101 aggttcagtt gtagccaacc ggtcgcaaac agcccgttga aaatcggcgt attcattacc
    26161 gcgtcaaaac ctgattttgc aaggctgctt ttcatactat caagcggggt agcttgtgca
    26221 atgccatcaa cactattaac tagctgatga atactaaaac catcgctagt taccccgtaa
    26281 aaaatcaacg aaatcgcatc acgaaatgtc ggtggttcac ttaataactc aatcggcact
    26341 atccacgccg tcatttgcac cgggaaagaa accaacaaca acgcatagcc aaccattgcc
    26401 gggttaaata gattctgccc caagccacca taactgtgtt ttgccaataa taacgccaca
    26461 atcacgccaa tcaacacaag ccaataagga gcataaggcg gaatcgacat cgccaaaatc
    26521 aaggcggtta aaatacctga taagtcagcc aaatagaaag cggttgtttt tttgcgtaat
    26581 tttgcaatcc cgatttcaat cacaatcgca aggctaacgg caagcaacgt ttgaatcagc
    26641 acaccatagc cgaaataata aagctgaacc ccaaaagcag gtaacatcat cgcaatcacc
    26701 cacagcataa aattcgcagt gagattgctc gaatgagtgt ggggggaact gaccatttta
    26761 aacatctata aaaccttttt agtaaaaaat tgcaaaaaaa tgaccgctta ttaaagtctc
    26821 ggcaaattca aattaaagcg tattgccagc aggcggataa taattacggt gaacacggtg
    26881 gcaaggtaaa cccaatcgct ttcgagccca actgagtttg cagccacaaa aatcacacca
    26941 ccgataaggc tggcggtcac atacacttct ttttgcaaaa tgatcggcac ttcgttgcgt
    27001 aaaatatcac ggattaagcc accaaaacag cctgtcacac cgcccagagc cacacagatc
    27061 agtgggtgca agccgaaatc caagcctttt tgcacgccaa tcacggtaaa tacgccaagc
    27121 ccgatggcat caaacaccag caagccggtt tgccaacttt tgcgggaaat ttggttgcgg
    27181 aaaatagcgg taatcaccac tgcaagggta atcatcagca catagatcgg ttgtttcatc
    27241 caaaacaccg gcgtggagcc aatcatcata tcccttagcg taccgccccc gaccccggtc
    27301 acaaacgcta aaataaacat accgaaaatg tccattttgt gctggcgggc agcaattgcc
    27361 cccgaaatcg caaacacgat agtgccgatt aagtcctgaa taaacaggaa cgaccacggc
    27421 aatgccggaa ttagctcgct aaacattgtt gcttttctcc tgctgagcgg cttttttcgc
    27481 tttagctctg gcgatggcgg ccgcaacggc ggctttgcgt gggtcttcgc tttccgtttt
    27541 gggcgaatgg ttattcgccc ctacagattt atccgatgta gtttcgttca ttgattgtag
    27601 gggcaaatca ttatttaccc caatcacagc attggcaata tcttccggtt tttcgaccgc
    27661 ttgttgagct tgttttttcg ctttagctct agcgatagct gctgcaacgg cggctttgcg
    27721 tgggtcttcg ctttctgttt cgggcgaatg gttattcgcc cctacagatt tatccgatac
    27781 cgtttcgttc attgattgta ggggcaaatc attatttacc ccaatcacgg cattggtaat
    27841 attttctgat ttttcgaccg cttgctgtgc ctgttttttg gctttggctc gggcaagggc
    27901 agccgcaacg gcggctttgc gtgggtcttc gctttctgct tcgggcgaat agttattcgc
    27961 ccctacagat ttatccgatg cagtttcgtt cattgattgt agaggcaaat cattatttgc
    28021 cccaatcaag gcatttgtaa cattttccga tttttcgacc gcttgttctg cctgtttggc
    28081 taaacgtctg gctctgcgtt gtgccattaa atcgctgtta tcaggctcgc cgttaggttt
    28141 tacctgtact tctgccggta tttcttggac tgtttcaacc gcttttttcg ctttcaggcg
    28201 ttcaagagcc gcttttacag gatcaacacc acctgcattt gccacttctt cacggcgttt
    28261 ttccgccgct tggctaatgc gagcagttct ggcatctcgt tctttttgta agcgagcttc
    28321 tctcgcctca aagcggattt tagcctcttc cgctttgcgg gctttttcct caatttcctc
    28381 gatttttgct ttttcttggc ggaaatattg gattaacgga atgtagcttg ggcaaacgta
    28441 agcacaaacg ccacactcaa tgcaggcatc taaatggtat tctttcgatt tatcgtgatc
    28501 ttctgcacgg gcaaaccaat aaagttgctg tggcaataac cccaccgggc aagcatcgga
    28561 acagcttgaa cagcggatac aactacgctc cggttcaggt ggtgcatatt caaaatgatc
    28621 aggggcaatc aagcagttcg cagttttggt aatcggggct tgcaatgcag gcaaaataaa
    28681 gcccatcatc ggtccgccta aaaacaccgg aaagcgttta tcggcttgat aatctacctg
    28741 ctccaacaag tgctgaattg gcgtgcctaa tcttgcccac acgttcccct tgttgcgaat
    28801 tttatcgcct gtcagcgtca ctacccgttc aatcaaaggt tcatcatcaa taattgcccg
    28861 tttaaccgca aacgccgtgc cgacattgtg catcacaatg cccatttcaa tagtgcgttt
    28921 gccagccgga atctcaagtc cggtcaacac ttgaatcagc tgatcagaag ctccggaggg
    28981 atatttagtc gggatcgccc gcacaaacat atcgttcgag ccttttaaag cggtttctaa
    29041 agcttgaata gcgtgcggtt tgttgtcttc aatcgcaatc accacttctt ccgggcgaag
    29101 cacataacgc agaatgcgaa taccttcgat aagatccgca gtgaaatctt gcattaagcg
    29161 atcgtcacag gtgatgtaag gctcacattc tgccccgtta atcaccagta atttacagcg
    29221 tttatccgcc aaacttaatt ttgttgcggt tgggaatacc gcaccgccta aaccggcaat
    29281 gccatattgg taaacttttt ggataatttg atcgcttgtt aggcttaaaa aatcctcaat
    29341 cggctggcgt tccacccatt tatctttacc atcggtttca ataatcaccg ttatttcggg
    29401 caaacctgag gcgtgagcgg aaacataagg ctcaatccca atcacccgcc cggaggttgg
    29461 gctgtgtacg ggcagttgac ggaagtgatc gcctttcgtg agcggctgcc ctttcaatac
    29521 cacatcgccg actttgacta ataattcccc tgcccagcca gaatgttgaa ccagtggcac
    29581 atagaaaaac ttcggtaaat ttaaggtgcg aattggcttg ctgtttgatt gggatttatt
    29641 ttccatcggg tgaataccgc ccgggaaatc ccacagttta cccgtttctt tattttggcg
    29701 gattcgctca atcactaaat taggattgct catttttgcc tccgccggta actaattttc
    29761 tctgcaattc agtggtattc acaatcggaa taattaaatc ttggtcaaat ttccagttcc
    29821 aagattgggt tgtcggttta attggtcgca tttcaataca gttggtcggg caaggggcga
    29881 cacaaagttc acagccggtg caaagatccg gaataatggt gtgcattgct ttattcgtgc
    29941 cgataatcgc atcaaccggg caagcctgaa tgcacttagt acagccaatg cagagatctt
    30001 caatcacaaa agccactttc atctcaggct ctgccatacc ttccattggc ggcacttcta
    30061 ccccaagcaa ttcggcaaga ttcaccacta aaggttgtcc acccggcaca catttagtga
    30121 tcacatcacc attagcaatc gcctcagcat aaggcttaca gcccgggtag ccacattgac
    30181 cgcattggct ttgtggtaaa agggcatcaa tttgatcgac aatcgggtcg gattcaactt
    30241 tcagtttaat cgaggaatag cctaaaaccg ccccgaaaat cagtgcgatc acagcaatag
    30301 cgataagaat ataagcgata attggcaaat caagcattag agtttcacca atcctgtaaa
    30361 gcccataaag gcaagcgaca tcaagcctgc ggtaatcaga gcaattgatg ctccttggaa
    30421 cggacgaggc acatcagcgg cagccaaacg ctcacgcaga gccgcaaata aaactaacac
    30481 cagcgagaaa ccggcagcgg ctccaaaacc ataaagaaca gattccacca agttattggc
    30541 taaattcacg tttaataatg ccacccctaa tacggcacag ttggtggtaa tcagcggtaa
    30601 atagatccca agcaagcgat aaagtgtcgg gctggtttta tgcacaatca tttccgttaa
    30661 ttgcacgatc accgcaatca ctaaaataaa caccaaggta cgcaaaaatt gagcgtttaa
    30721 cggctcaagc aaataggttt ccatcaaata agcagagagc gaggcaaccg tcagcacaaa
    30781 ggttgtcgcc aaccccatac caatagcagt ttccaccttt ttcgacactc ccataaacgg
    30841 acaaagccct agaaatttaa ctaagacaaa gttattaata agggcggtgc tgatgaccaa
    30901 cagaatataa tcgaccataa attccacaaa attaaatata caagcggtca aaaacgcatt
    30961 ttattttgca aaaaacagag aaaatctgac cgtttgttaa agcatctcat taaaaataaa
    31021 cgttatatta tctcttgttt tcggctcatt cacaataaga aatcggaggt acttctagta
    31081 aacataccat atcaaatatg atcttgttac ctttgtttct atctctggta gaatggagaa
    31141 tgtggggact aagctataca gcatgaaagg tgaaaggagc ttgattttct aagttaatcc
    31201 taaaaagtct cactcctttg atatagagga tattagaata acaaccaatt aattactcga
    31261 taaataaaca taataatccc aaatataatg aaaacataat aaatcttttt ccataaaggg
    31321 agttttacct cttcttcaga atattcttca tc
//
LOCUS       DANLRK010000007        31245 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.007, whole genome shotgun sequence.
ACCESSION   DANLRK010000007 DANLRK010000000
VERSION     DANLRK010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 31245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31245
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.007"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            265..966
                     /locus_tag="PXA33_000223"
     CDS             265..966
                     /locus_tag="PXA33_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL5119604.1"
                     /translation="MIGQALGWRATFGIIGIIALIVLMMIWRLLPYLPSKNAGSIKSL
                     PLLFKRPMLIGIYLLVALIVSGHFTAYSYIEPFMIKISSVSVENTTFILLVFGLAGVV
                     ASFLFGRLYGKAPNKFILSGIAIIMCSQLCLLFLSDSTVAMFMVAFVWGIGITGMSIA
                     LQMKVLELAPDATDVATAIFSGTYNLGIGGGALLGSIAIQQLGLANIGFAGGSIAFVA
                     MVWFVFVSLKYRQLR"
     gene            complement(1033..2766)
                     /locus_tag="PXA33_000224"
     CDS             complement(1033..2766)
                     /locus_tag="PXA33_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3413 domain-containing protein"
                     /protein_id="HDL5119605.1"
                     /translation="MFARLRSLLPTNSRQYREETSQKITWGHWFALFNIVLALLISSR
                     YAFNADWPNTLVGKLYFFVSLFGHFSFVVFAGFLLILFPLSFIVKNHRTYRGLSVILA
                     TIGQTVLLVDTEVFKQFYLHLSPLVWDLLVNPEEGELTRKWQLLFVPMPLLLLAEMLY
                     SRWCWQKLRSFSRQKWGKYVALFFVISFTATHLIYAWADMNAYRPITAQRANYPLSYP
                     MTARNFLEKHGFIDRVEFNQEIEESGRLDTFYLNYPKSPLIATKTPSTNILLINLSGL
                     SKQIINAETMPKLTEISQQSHRFMQHYSSGDSASAGALGLFYSLSGKYLDSVLADKTV
                     SPLIEIARKSDYQLGLFSHNGFADPIYHQAIFSGILLPTAHSNVGAIGQWKNWISSRS
                     LENPFFSYLDLRMPFAHTAQEQAKLLDWQFAEIWQHLTQNALLANTLVIITADISNEV
                     GAENSFDKQHIQVPMMFYWQGESKEYHFLSSHLDIMPTLLSQFFGVQNPLNDYAQGMD
                     LSTQNQRLWVSSSNHKWNVAIMPNGDQYQIDRKGNFKHFNSNGEHEKDARPPLALFLQ
                     MIQQSNQFIEK"
     gene            complement(2771..2992)
                     /locus_tag="PXA33_000225"
     CDS             complement(2771..2992)
                     /locus_tag="PXA33_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1414 domain-containing protein"
                     /protein_id="HDL5119606.1"
                     /translation="MATKSKYHTKQFEQLLADLIATIEKHKAPVDLSLMALGNMITNI
                     LIENIQSPAQREALADAFADALKQSLKTK"
     gene            3231..3803
                     /locus_tag="PXA33_000226"
     CDS             3231..3803
                     /locus_tag="PXA33_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL5119607.1"
                     /translation="MSRRSEPKEEMVNRILSATESLIVQEGLQNLSMRSIAKEAGIAS
                     GTLYLYFKTKDDLLHSLAAQLLECYNGYMHFEFKPEMALFEQYKALVNKKWQFLIERL
                     DLVKQFQAVIGIDELIRETIADETSFWNKLILEGKKQRIIADLSNELLYGLSIGTLVD
                     IQYLQNLNQENQFDGYLDEIILRTWKAITF"
     gene            3834..5039
                     /locus_tag="PXA33_000227"
     CDS             3834..5039
                     /locus_tag="PXA33_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HDL5119608.1"
                     /translation="MTTETQKLGKGRKFLIVLTLLIVLIVFAAIVGLQKIGAMKKAEA
                     AANMPEAVSEVTAMQVTTQEWTPTISAVGYIRPNQGAMLSAETSGVVSRVLVTSGQRV
                     KKGDLLVEFDSSVEIANLRASEAQLPNAKANLERYRNLIASNSASKAEFDNAQSTYNQ
                     LLANIEALRATIKRRQIYAPFSGLAGIVNVNVGQYITMGTEIVRVEDQSSMKVRFTLP
                     QTNLEQISIGQKVTAVIDALPAQTFPAKIVAIDPAVDRLTGLINVEAVIEEGQEKLLS
                     GMFARLNVALPTETGQIVVPQISIAYTMYGETVYVLQPLSDEDKEMVNKMAVQNPSLD
                     VNKMYRAKQMEVKTADRKGNYSQLSKGVKAGDLIVTGGLQRLSNNALVKVSDTEAVGI
                     TTPAKNSKL"
     gene            5053..8220
                     /locus_tag="PXA33_000228"
     CDS             5053..8220
                     /locus_tag="PXA33_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HDL5119609.1"
                     /translation="MKFTDIFIKRPVLAVCISLLITILGLQAIEKLQVREYPEMTVSI
                     VTVSVNYSGADARLMQTLVTSQLEEAVAQADNIDYMTSSSSPSTTTVTAKMKLNTDPN
                     AALSDISAKVNAVRSNLPSGIDDPSIRVSTGSNDALMYIRFVSDELNTSQVTDYINRV
                     VKPQFFTVNGVSSVDVYGSVFGLRIWLDPDKMASNNLSGAAVLSALSANNLQTAAGNS
                     NGYYTVYRNKVLSTTPSVTELEEVTVSTTKDGKTIKLKDIATVELDKYQDAARATVGG
                     KDAVVLAVSVAATANSLTVAKDIYPMFEQVLKNLPSTIQGDIVYDKTIAIDSSIKEVI
                     KTIGEATVIVLVVIILFLGSLRAMIVPVITIPISLIGVLFFLQMFGFSINLLTLLALV
                     LAIGLVVDDAIVVLENVERHVKEGKTPFDAAIIGTREIALPVISMTITLGAVYSPMAL
                     MTGVTGTLFKEFALTLAGAVFISGIAALTLSPMMSSRILKEHKEGEESAFARVVHRSL
                     DKMTSCYTNMLSGIMAVRGFVLFFAVLIFASLPMLFGSLSSEVAPTEDRGFVVGISNG
                     PSNTNLDYTEAALAQFNQEVTKLPEVSSLMTVSGFNGANSALSIIALKDWNDRSRERA
                     AISNDVAAIGKKVVGMDVNAIAFPEISTGENGLPFSLVITTADSYEKLAIVASEFLKK
                     AQTSGQFFFANLDLKFDTVTMNMKFDREKMGAYGVTMQQVSGTLGAFLSGAIITRVDI
                     DSRAYPIVSQAVRNDRLNPEDLMNYYVTTATGQSIPLGSFVTMELKTSPSSLPRMSQL
                     NSATIGGVVSGSIGDAVNWAKAELDRTLPSGYQYDFRGESRQFVQEGNAMAMTFALAV
                     IIIYLVLAIQFESWRDPLVILVSVPLAVSGALLVMNIPTAMKGLFMGIAAGTGSKDMM
                     AFANGIWTAGYTLNIYSQVGLITLVGLITKHGILMCEVAKEEQLLHGKSRYEAIFHAA
                     TIRLRPIMMTTAAMIAGLIPLLFAVGAGAIARFSMGMVIVAGLAIGTLFTLFILPVIY
                     TFLGQEHKPLREFDEEKYFPTTKEA"
     gene            8448..9911
                     /gene="guaB"
                     /locus_tag="PXA33_000229"
     CDS             8448..9911
                     /gene="guaB"
                     /locus_tag="PXA33_000229"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005725353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HDL5119610.1"
                     /translation="MLRIKQEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTETKLAISLAQEGGIGFIHKNMSIERQADRVRKVKKFESGIVSEPVTISP
                     DMTLAELAELVKKNGFAGYPVIDENQNLVGIITGRDTRFVTDLSKTVREFMTPKDRLV
                     TVKENASREEIFHLMHEHRVEKVLVVNNEFQLKGMITLKDYQKAESKPNACKDEFGRL
                     RVGAAVGAGPGNEERIDALVKAGVDVLLIDSSHGHSEGVLQRVRETRAKYPDLPIVAG
                     NIATAEGAIALADAGASAVKVGIGPGSICTTRIVTGVGVPQITAIAEAAAALKERGIP
                     VIADGGIRYSGDISKAIAAGASCVMVGSMFAGTEEAPGEIELYQGRAFKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRIAYKGYLKEIIHQQMGGLRSCMGLTGCATI
                     EELRTKAEFVRISGAGIKESHVHDVTITKEAPNYRMG"
     gene            10032..10703
                     /locus_tag="PXA33_000230"
     CDS             10032..10703
                     /locus_tag="PXA33_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDL5119611.1"
                     /translation="MLQAIIFDMDGVIVDTEYLEFSLQKQFIEDIKEHSRPITLEQQS
                     EVVGKCLKEIPVIIKKLSESSLPIEEIRARYYAFFQDLFSKVDFKTIFRADIQQIIQF
                     AKQNQIKLAVASSSALSHIENILTVCGIKDEFDLIVSGEQFEHSKPDPTIYRYTCEKL
                     GVEPQNAVAIEDSYYGMLAAKTAGLTVIGYEEKRILIDQSLADYMGKDMKEILKILQK
                     CNTPS"
     gene            10825..11136
                     /locus_tag="PXA33_000231"
     CDS             10825..11136
                     /locus_tag="PXA33_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5119612.1"
                     /translation="MKKLSLISIVALSACSIFGPSYSGETTASPLLKLDTERNINLYF
                     QAIHKCTPDKIHTKIINIPSEGNAKELWTATGCNKTQSFIITYRNDGVGGTFINMTTT
                     P"
     gene            11153..12724
                     /gene="guaA"
                     /locus_tag="PXA33_000232"
     CDS             11153..12724
                     /gene="guaA"
                     /locus_tag="PXA33_000232"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012510808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HDL5119613.1"
                     /translation="MTNIHHHKILILDFGSQYTQLIARRVREIGVYCELWAWDVTEEQ
                     IREFNPTGIILSGGPESTTEENSPRAPEYVFNAGLPVLGICYGMQTMAMQLGGLTETS
                     DHREFGYASVALKSADQLFANLNDDLTACEPKLDVWMSHGDKVTKLPPHFTITGVTPT
                     CPIAAMSDESRRFYGVQFHPEVTHTKQGLALLKNFVVGICGCETNWTAENIIEDAVKR
                     IREQVGDDEVILGLSGGVDSSVTALLLHRAIGKNLHCVFVDNGLLRLNEGDQVMEMFG
                     NKFGLNIVRVNAEDRFLEALKGLDEPEAKRKTIGKVFVDVFDDESKKLSSVKWLAQGT
                     IYPDVIESAASKTGKAHVIKSHHNVGGLPDYMKLGLVEPLRELFKDEVRKIGLALGLP
                     AEMLNRHPFPGPGLGVRVLGEIKKEYCDLLRKADAIFIEELHKAGWYYKVSQAFTVFL
                     PVKSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDLLGKISNRIINEVNGISRV
                     VYDVSGKPPATIEWE"
     gene            complement(12885..13280)
                     /locus_tag="PXA33_000233"
     CDS             complement(12885..13280)
                     /locus_tag="PXA33_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcB family protein"
                     /protein_id="HDL5119614.1"
                     /translation="MEQWTTDVWAAIMAAFIVGAVIGCTLVRVFNSSVRKQHELEKEL
                     KQTKTQVDEQKQKLEQHFEQSAQLLSSLASDYKKLYTHLAQSSEKLLPEASKNITFFQ
                     QTKLPIPAEKTEEDQPRDYSEGSSGLLKS"
     gene            13579..14007
                     /gene="rplM"
                     /locus_tag="PXA33_000234"
     CDS             13579..14007
                     /gene="rplM"
                     /locus_tag="PXA33_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HDL5119615.1"
                     /translation="MKTFVAKPETVQRDWYVVDATGKTLGRLATELARRLRGKHKPEY
                     TPHVDTGDYIIVINADKVAVTGKKQTDKIYYWHTGYVGGIKDATFKEMIARRPEAVIE
                     IAVKGMLPKGPLGREMFRKLKVYAGSEHNHQAQQPQVLDI"
     gene            14024..14419
                     /gene="rpsI"
                     /locus_tag="PXA33_000235"
     CDS             14024..14419
                     /gene="rpsI"
                     /locus_tag="PXA33_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015432457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDL5119616.1"
                     /translation="MTAANQNYGTGRRKSSSARVFIKPGSGNITINQRSLEVYFGRET
                     ARMIVRQPLELVELTDKLDLYITVKGGGISGQAGAIRHGITRALMEYDETLRPALRAA
                     GFVTRDARRVERKKVGLHKARRRPQYSKR"
     gene            16101..17821
                     /gene="ltrA"
                     /locus_tag="PXA33_000236"
                     /pseudo
     CDS             16101..17821
                     /gene="ltrA"
                     /locus_tag="PXA33_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012341372.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            18010..18786
                     /gene="yaaA"
                     /locus_tag="PXA33_000237"
     CDS             18010..18786
                     /gene="yaaA"
                     /locus_tag="PXA33_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005622827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HDL5119617.1"
                     /translation="MLAIISPAKTLDFKTNIDGFAFSQPNLTACSQELIDICKQLSPA
                     ELGSLMSISDKLAALNVARFAEWQLEHNEQNAKAALFAFKGDVYTGLEAETLSKAQVE
                     YAQQHLRMLSGLYGLLKPLDLMQPYRLEMGTKLANPKGKDLYAFWGNIITENLQQAID
                     EQGDDILVNLASDEYYGAVKPQNLNATIIKPVFLDEKNGKYKVISFYAKKARGMMVRF
                     ILDTQPTSIEQLKQFNYGGYWFDADSSSESELVFKREEQA"
     gene            18786..19349
                     /locus_tag="PXA33_000238"
     CDS             18786..19349
                     /locus_tag="PXA33_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATPase"
                     /protein_id="HDL5119618.1"
                     /translation="MSAIKKGITLVALSLAGCVSQSPQFAVPDAIHFGQDTFVKVTHS
                     QIDEMQHLLYLPEKSEKNPENWQKGILFFLDKNSKNQTLEQRAAFRQASFAKKPELEA
                     KVEIQQNELHSSVIYPPIERFNNVMLEVTRGRNLACGYGQIQFADKREVGKTVAKKSP
                     NLTAYSQEIARLSLELNQLGWQVQCSK"
     gene            19358..20263
                     /locus_tag="PXA33_000239"
     CDS             19358..20263
                     /locus_tag="PXA33_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HDL5119619.1"
                     /translation="MNEKYSKLVKNAANLAIIVAILLIVIKAFAWWKTGSISILAAMT
                     DSVVDLFASLTNILVLRFALQPADENHAFGHGKAESLAALAQSAFISGSAVFLLLQGF
                     HKLTNPELIAQSSIGVIVSIVSIVITALLVFYQRYVVTLTKSPAIEADSLHYQTDLLM
                     NAAILVAMLLNSAGFVYADAVFAIGIALYIAFNAVKMFWCAVQTLLDQALPDEEIERI
                     KEIASQHPNILGIHDILTRRAGAVRFIQLHLELDDHLTLLVAHEITDSLEQKLLAAFP
                     MSHVIIHQEPTTVVKQEFQDMAENI"
     gene            20263..21015
                     /locus_tag="PXA33_000240"
     CDS             20263..21015
                     /locus_tag="PXA33_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon-containing protein"
                     /protein_id="HDL5119620.1"
                     /translation="MYQAIVHFSYHNFEQDPVTAISQILNQWLYNGQVIGREMPITFH
                     QNEFQARVCVPTQESLLPQYNSDEVNEALQQAVEIGVIFASFELVGRDYQGEESSHNR
                     SPAFQILYTTHLDTCSPLYDGEQFAPIPLYSLQDQSLSEAILEWQQNWQACDLLQMNG
                     NLLECHALVQISDETSDLAMIGRDLCQQIEQKTHIPTFYYLYRLGRDESEYQRKCPCC
                     GGEWKLTEPLHDIFHFRCEPCRLISNLSWELL"
     gene            21116..22441
                     /locus_tag="PXA33_000241"
     CDS             21116..22441
                     /locus_tag="PXA33_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HDL5119621.1"
                     /translation="MLIFNLIVFAAILFLLAKIYKSTQKLGQTVFIGLFLGLASGAFL
                     QTFYEKATVDATLDWINLIGNGYVRLLQMIVMPLVFVSILSAITRLKEAGSLGKISFS
                     VLSVLLVTTAIAAAIGIALVYLFDLSAEGLVAGSRELAAQEKVVGRVEQVSNLSVPSM
                     LLSFIPKNPFLELTGANPTSIINTVIFSALLGIAALSLGKEDKALGERIAQGVETLNK
                     LVMRLVRFVIRLTPYGVFALMIKMAATSQWSDIVNLGSFIVASYIAIGLMFIVHGILL
                     FVAKINPLDYYKKVLPTLSFAFTSRSSAATIPLNIETQTNKLGNNSVIANFSATFGAT
                     IGQNGCAGIYPAMLAVMVAPTVGMDPFSLNYILTLILVVAISSFGIAGVGGGATFAAI
                     VVLSTLNLPIELVGLLISVEPIIDMARTALNVNGAMVAGTLTNKWVKAE"
     gene            complement(22501..23160)
                     /locus_tag="PXA33_000242"
     CDS             complement(22501..23160)
                     /locus_tag="PXA33_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDL5119622.1"
                     /translation="MFNISKQQVLDAFHFRSACRSYDPNKKINKEDMDYILELGRLSP
                     SSVGSEPWKFLVLQNPETRQKIAPVSWGIKHPMEEMSHLVVILTKKNVRYDSVYMRDM
                     LAKRGLTYEQIEASLVRYKSFQEEDMKLLSERELFDWSTKQTYIALGNMMTGAALIGI
                     DSCPIEGFHYDTVNQILSDEGLFDLNEYGVSCMITFGYRNKEIKKKSRKPTEEVIAWI
                     E"
     gene            23389..24375
                     /gene="pheS"
                     /locus_tag="PXA33_000243"
     CDS             23389..24375
                     /gene="pheS"
                     /locus_tag="PXA33_000243"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HDL5119623.1"
                     /translation="MQHLKDLTNKAREALDKIDQNLEALEEFRVEYFGKKGHFTTLMQ
                     ELRNIAAEERPAIGAKINEAKQVVLDVLNAKKEEWEQAVLNAKLANESIDVSLPGRKT
                     ELGGLHPVSITIERVVKFFSELGFTVANGPEIETDYYNFDALNIPDHHPARADHDTFW
                     FDANRLLRTQTSGVQIRTMENVKPPIRIVAPGRVYRNDYDQTHTPMFHQIELLYVDKE
                     ANFTELKGLIHDFLKAFFEEDLQVRFRPSFFPFTEPSAEVDVMRQNGKWLEVLGCGMV
                     HPNVLRNVGIDPEEYSGFAVGMGVERLTMLRYNVTDLRSFFENDLRFLKQFK"
     gene            24423..26810
                     /gene="pheT"
                     /locus_tag="PXA33_000244"
     CDS             24423..26810
                     /gene="pheT"
                     /locus_tag="PXA33_000244"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HDL5119624.1"
                     /translation="MKFNESWLREWVNPAISTEQLCDQITMLGLEVDDVEPVAGAFSG
                     VVVGEVVECAQHPDADKLRVTKVNVGGERLLDIVCGAPNCRQGLKVACAVEGAVLPGD
                     FKIKKTKLRGQPSEGMLCSYSELGIKEDHSGIIELPADAPIGQDFREYLNLNDVAIEI
                     SLTPNRADCLSIAGIAREVGVINRVDVKAPTITDVKATISDKVSVELQAPEACPRYLA
                     RVVKNVNVKATSPLWLQEKLRRCGIRSIDPIVDITNLSLLELGQPMHAFDAAKIEGGI
                     QVRMAKEGEELVLLDGTNAKLQSNTLVIADSKGALAMAGIFGGEASGVSENTKDVVLE
                     SAFFAPLAITGRARQYGLHTDASHRFERGVDPELTRQAMERATALLLEICGGEAGEIV
                     EAVSQENLPKRNTVTLRRSKLDSVIGHHIDDATVTDILTRLGLNVTFADNSWTAIAPS
                     WRFDIEIEEDLIEEIARIYGYNSIPNNSPLAHLTMKGTPEKLLEANRIRMALVDSDYQ
                     EVITYSFVDPKKQSLLHPQQEALILPNPISSEMSAMRVSLLTGLLDTIVYNQSRQQNR
                     VRIFEAGLRFIPDSNAESGVRQEFVFGAAIVGDKRPVHWENKSEAADFFDLKGDIERI
                     LSLTSIRHDLQFVAKQFPALHPGQSAAIMLDGKEIGFIGTVHPSIVQKFGIKGKPIVC
                     EILASAIAERPVTQAGEISKFPANNRDIAVVVDESVPAGEVLEACRKAGGNKLVAVNL
                     FDVYRGSNLESGKKSLAISLTVQDNEKTLEENEINAVIQAVLAELATRFNAVLRD"
     gene            26868..27164
                     /locus_tag="PXA33_000245"
     CDS             26868..27164
                     /locus_tag="PXA33_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005710633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HDL5119625.1"
                     /translation="MALTKIEIAENLIEKFGLEKRVAKQFVELFFEEIRSSLENGEEV
                     KLSGFGNFSLREKKARPGRNPKTGENVAVSARRVVVFKAGQKLRERVEQASSQS"
     gene            27203..27724
                     /locus_tag="PXA33_000246"
     CDS             27203..27724
                     /locus_tag="PXA33_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDL5119626.1"
                     /translation="MKLTKRWLSLGCLLAITAMLVACSSSDTTSKSQVKSKVYRSIYQ
                     SDLSDPIMAISRLSEHQHEWKGTRYRLGGNSKAGIDCSGFMQVTFKDLFGIDLPRTTT
                     EQAKAGERISKDELRTGDLVFFKTGRGPNGKHVGVYVKNGQFLHASTKGGVIYSDLDS
                     PYWSKAFWQARRL"
     gene            27740..28783
                     /gene="degS"
                     /locus_tag="PXA33_000247"
     CDS             27740..28783
                     /gene="degS"
                     /locus_tag="PXA33_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HDL5119627.1"
                     /translation="MLKKIGQAVIFGLFCAILILFVAPIIQSKGSSFSLSAFTQKEIV
                     SYNAAVKIASPAVVNVYSRNFSSENETFEVKNLGSGVIMSSDGYILTNRHVIEDADQI
                     VIALQNGAISTNVKLVGDDSLTDLAVLKIEGENLPTIPQNPERKLQIGDVVLAIGNPL
                     NLGQSITQGIVSAIGRKTLTESGRQNFIQTDVSINQGNSGGALINTAGELIGINTLRF
                     GKNTNELSEGLNFAIPINLANQIMSKIIKDGRVIRGYFGVSTDLLDTAKMQGYEKGVI
                     IVNVDKNGPAAKAGIQAGDLVLKVGDIEAFSPSQMMEELAEMKPYTTVKVLVQRDEQL
                     LNFDVTITELQTQ"
     gene            28792..29466
                     /gene="cmk"
                     /locus_tag="PXA33_000248"
     CDS             28792..29466
                     /gene="cmk"
                     /locus_tag="PXA33_000248"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HDL5119628.1"
                     /translation="MKKIVITVDGPSGAGKGTLCHALAEKLGFDFLDSGALYRITALS
                     AIKKEVPLDDEEKLAEVGRNLDIQFLPENGEIQVILEGENVGDLIRTAEAGQNASKVA
                     AFPLVREALLERQRAFSSEKGLIADGRDMGTVVFPDAQIKLFLDASAEERTKRRVKQL
                     QEKGFNANFDEILAEIKERDFRDRNRSVAPLVPAKDALLLDSTHLSIEDVINQALNHI
                     AQYIKF"
     gene            29558..31225
                     /gene="rpsA"
                     /locus_tag="PXA33_000249"
     CDS             29558..31225
                     /gene="rpsA"
                     /locus_tag="PXA33_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006717605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HDL5119629.1"
                     /translation="MSELSFAQLLEESFAATPRLGDVVKGTVVAIQKGLVIVDTGFKS
                     ESAIDASEFTNAQGELEVQVGDEVDVVLKAVEDGFGATVVSRGDAKRNEAWIALEKAF
                     EEQATVIGFVNGKVKGGFTVELNGVRAFLPGSLVDTRPVRDSLNLEGKEYEFKVIKLD
                     QKRNNVVVSRRAVIETESNQDRDAVLANLVEGSEVKGTVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEVVNVGDEVTVKVLKFDKDKTRVSLGLKQLGQDPWAVIAENHPV
                     NSKLTGKVTNLTDYGCFVEILDGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLE
                     VDEERRRISLGLKQCKANPWEQFAATHSKNDKVSGKIKSITDFGIFIGLEGGIDGLVH
                     LSDISWNVSGEEAVRNYKKGDEVEAVVLQVDAVKERISLGIKQLESDPFTNFVDGTKK
                     GAILTGKVVEVDAKGVKVELEGGVEAFIRANEATAERVEDITSVISVGDSIEAKYTGV
                     DRKSRVVNLSVRAKNEAEESAAIAQVNKEEVVVPNAFAEAFKAAKGE"
BASE COUNT         9408 a         5664 c         7095 g         9078 t
ORIGIN      
        1 gaaacttgct cgaaaagctc aacggagatt atcgttcctt agagtattta gtcttgcctt
       61 tgcggctttt atagtaaaca ccaccgaatt tattcctatt gcattgcttt cagacattgc
      121 aagcagtttc caaatggagc cttcatcggc aggcattatg atgacggttt atgcgtgggt
      181 ggtattttta ttatcgttac cgttgatgtt actcacggcg aatatggaaa gaaaacggct
      241 gttgctgatg ttatttatgg gcgaatgatc gggcaagcct taggctggcg ggcaaccttt
      301 ggcattattg ggattatcgc tttgattgtg ctaatgatga tttggcggtt gctgccttat
      361 ttaccaagca aaaatgcagg ctcgataaaa agtttgcctt tgttatttaa acgcccgatg
      421 ttaatcggca tttatttgct ggtggcattg atcgtttcag gacattttac cgcttacagc
      481 tatattgagc cgtttatgat taaaatcagt tcggtgagtg tggaaaacac cacctttatt
      541 ttgctggttt ttggcttggc aggtgtggtg gcaagttttt tatttggtcg gttatatggc
      601 aaagccccga ataaatttat cttaagcggg atcgccatta ttatgtgcag ccagctttgt
      661 ttactgtttt tgagtgactc aacagtggca atgtttatgg tcgcttttgt ctggggaatt
      721 ggcattaccg gaatgagcat tgcgttgcaa atgaaggtgc ttgaacttgc ccctgatgcg
      781 actgatgtcg caactgcaat tttttccggc acttataatt taggcattgg cggtggagca
      841 ttactgggga gcatcgctat tcagcaactg ggattagcca atatcggatt tgccggtggg
      901 agtattgctt ttgtagcaat ggtttggttt gtatttgtta gcttgaaata tagacagctc
      961 agatagtgac aagcggtcat attttgctga aattttgcaa attttcctgc aaaaatgacc
     1021 gcttgttgaa cattactttt caataaattg attgctttgt tgaatcattt gtaagaacaa
     1081 cgctaatggt ggtcgagcat ctttctcgtg ttcgccattg ctgttaaagt gcttaaagtt
     1141 gcctttacga tcaatttgat attgatcgcc attcggcata atcgccacat tccatttgtg
     1201 attagaactg gatacccata aacgttggtt ctgggtgctt aaatccatac cttgtgcata
     1261 atcattcagc ggattttgca cgccgaagaa ttggctcagt aaggttggca taatatctaa
     1321 atggcttgaa aggaaatggt actctttgct ttcgccttgc cagtagaaca tcatcggcac
     1381 ttgaatgtgt tgtttatcga agctattttc tgctccaact tcattactaa tatcggcggt
     1441 aatgattacc agcgtattcg ccaatagtgc attttgggtc aaatgttgcc agatttcagc
     1501 gaattgccaa tctaataatt ttgcctgctc ttgagcggtg tgggcgaagg gcattcttaa
     1561 gtctaaatag ctaaagaacg gattttccaa tgagcgagag ctgatccagt ttttccattg
     1621 cccaatcgcc cctacattac tgtgcgccgt aggcaataaa ataccagaga agatcgcctg
     1681 atgataaatc gggtcggcaa aaccattatg tgaaaacagc cctaattgat agtccgattt
     1741 tctagcgatt tcgataagcg gtgacacggt tttatcggct aaaacggaat ccaaatattt
     1801 accgcttaaa ctgtaaaata gacctaatgc accggcagag gctgaatcac cactactgta
     1861 atgctgcata aagcggtggc tttgttggct gatttctgtc agcttaggca ttgtttcggc
     1921 atttattatc tgtttagata aacctgataa attaatcagt aaaatattag ttgagggcgt
     1981 tttggtcgca atcaagggag attttggata attcagatag aaagtatcta gtctgccact
     2041 ttcttcaatt tcttgattaa attctactcg gtcaataaaa ccgtgttttt ctaagaaatt
     2101 acgagccgtc atcgggtaag aaagtgggta gtttgctcgt tgagcggtaa tcgggcggta
     2161 ggcattcata tctgcccacg catagattaa atgagtagcg gtaaagctga tcacgaaaaa
     2221 gagagcgaca tatttgcccc atttttggcg gctaaaacta cgcagtttct gccagcacca
     2281 gcgagagtag agcatttctg ccagcaaaag gagtggcatt ggtacaaaca gcagttgcca
     2341 tttacgggtc agttcccctt cttccggatt aactaacaga tcccatacaa gcggtgataa
     2401 atgcagataa aattgcttga agacttcagt atccaccaat aacacggttt gcccgatggt
     2461 ggctaaaatc acggaaagcc cacgataggt gcgatgattc ttaacaataa aactgagcgg
     2521 aaacagaatt aatagaaatc ctgcaaagac cacaaagctg aaatgcccga acaggctgac
     2581 aaagaaataa agtttgccta caagcgtgtt cggccagtct gcattaaatg cgtaacgaga
     2641 ggaaatgagt agtgccaaca caatattaaa tagtgcaaac caatgccccc acgtgatttt
     2701 ctgtgaggtt tcttctcgat attggcgaga attggtcggc aataaactgc gtaggcgtgc
     2761 aaacatcatc ttatttggtt tttagtgatt gttttaatgc atcggcaaag gcatcagcga
     2821 gagcttctct ttgtgccggg ctttgaatat tttcaattaa aatgttagtt atcatattgc
     2881 ctaatgccat taacgataaa tccaccggtg ctttatgctt ttctatggtg gcgatgaggt
     2941 cagccaatag ctgctcaaat tgtttggtgt ggtatttaga tttcgttgcc atattgaaat
     3001 aaagatgatc ctataattag gtacaaatag tagcacaaaa ataataataa agtagcgtta
     3061 agcgttattg tttgagtgat ttttgcaaag ttttagccaa aaatgaccgc ttgttcaata
     3121 aatgaggaat tttgcaacta ttttatttta atttgatagg tgttttctgc tttctttttt
     3181 cataaaaaaa gctataatga acggctgttc attataggta aggagataaa atgtctaggc
     3241 gaagcgagcc aaaagaagag atggtaaacc gtattttatc ggcaaccgag tcgctgattg
     3301 tgcaagaagg tttgcaaaac ctttcaatgc gtagtattgc aaaagaagca gggatcgctt
     3361 ccggtacatt atatctttac tttaaaacca aagatgattt attgcactca ttggctgccc
     3421 aattactcga atgttataac ggttatatgc attttgagtt taagcctgaa atggcgttat
     3481 ttgagcaata taaagcgttg gtcaataaaa aatggcagtt tttaatcgag cgtttagatt
     3541 tggtaaaaca gtttcaagcg gtgataggga ttgatgaatt gattcgggaa accatagctg
     3601 atgaaacttc cttttggaat aaattaattt tagaagggaa aaagcaaagg attattgctg
     3661 atctatctaa tgagttgctt tatggattaa gcatcggcac attggtggat atccaatatc
     3721 tgcaaaatct gaatcaagaa aatcagtttg atggatattt agacgagatt attttacgca
     3781 cttggaaagc aatcactttt taagtttctt tttattagtt ttatggagaa tgtatgacaa
     3841 cggaaacaca gaagctgggt aaaggcagaa agtttttaat tgtactcaca ttgttaatcg
     3901 tattgattgt gtttgctgca atcgtagggt tacaaaaaat cggggcaatg aaaaaagccg
     3961 aagcagcggc aaatatgccg gaggcggtga gcgaagttac tgctatgcaa gtgacgaccc
     4021 aagaatggac accaaccatt tctgcggtcg gttatattcg cccaaatcaa ggggcaatgt
     4081 taagtgctga aacttcaggc gtggttagcc gtgttttggt cacttcagga cagcgtgtga
     4141 aaaaaggtga tctgttagta gaatttgaca gcagtgttga aattgctaac ttgagagcct
     4201 ctgaagcgca attaccgaat gccaaagcaa atttagagcg ttaccgtaat ctgattgcct
     4261 caaacagtgc ttctaaagcc gagtttgata atgctcaatc tacctacaac caattattag
     4321 ccaatatcga ggcattgcgt gctacgatta aacgccgtca aatttatgct ccgtttagtg
     4381 gtttagcggg tatcgtgaat gtgaatgttg ggcaatacat tacaatgggt acggaaattg
     4441 tgcgtgtgga agatcaatct tcaatgaaag tacgttttac cttgccgcaa actaatttag
     4501 agcaaatctc gattgggcaa aaagtgacag ccgtaattga tgcgttaccg gcacaaacct
     4561 tcccagctaa aattgtggct attgatccgg ctgtggatcg tttaaccggc ttaattaatg
     4621 tggaagcggt gattgaagaa gggcaggaaa aattgctttc aggtatgttt gcccgcttaa
     4681 atgttgcttt accaacagaa accggacaaa tcgtcgtacc acaaatttct atcgcttaca
     4741 ctatgtatgg tgaaacggta tatgtgctgc aaccattatc tgatgaagat aaagaaatgg
     4801 tgaacaaaat ggcggtacaa aacccaagtt tagatgtaaa caaaatgtac cgtgcaaaac
     4861 agatggaagt gaaaacggct gaccgcaaag gaaactattc acaattatcc aaaggcgtga
     4921 aagcaggtga tctgattgta acaggcggtt tacaacgttt aagtaataat gcgttggtta
     4981 aagtttctga tacagaagcc gtaggcatta ccacaccggc gaaaaacagt aaactgtagt
     5041 ggagtgagtt aagtgaaatt tactgacatc tttattaaac gtcctgtttt ggcagtttgt
     5101 atcagcctat tgattacgat cctaggcttg caagcaatcg aaaaattgca ggtgcgtgaa
     5161 tatccggaga tgacggtctc gattgtaacc gtgagtgtta actattcagg tgcagatgca
     5221 cgtttaatgc agacactggt tacctcacag ttagaagagg ctgttgctca agcggataat
     5281 attgactata tgacttcatc aagctctcca agtaccacta cggtaacggc aaaaatgaag
     5341 ttaaataccg atccgaatgc cgcactttcc gatatttcgg caaaagtgaa tgcggtacgc
     5401 tcaaatttac cgagcggtat tgatgatccg tcaattagag tctcaaccgg ttcaaatgat
     5461 gcgttaatgt atatccgatt tgtctctgat gaacttaata catcacaagt taccgattat
     5521 attaaccgtg tagtaaaacc acagttcttt acggtaaatg gtgtttctag cgttgatgtg
     5581 tatggctctg tttttggctt acgcatctgg cttgatccgg ataaaatggc gagcaataac
     5641 ctatcagggg cggctgtatt atcggcatta agtgccaata acttgcaaac cgctgcgggt
     5701 aattctaacg gttactatac ggtttataga aacaaagtac tttctaccac gccttctgtg
     5761 acagagttag aagaggttac ggtttcaacc accaaagatg gaaaaaccat caaattaaaa
     5821 gatattgcga ctgttgagct ggataaatac caagatgcag caagagcaac ggttggcggt
     5881 aaagatgcag ttgtgcttgc tgtttcggta gccgcaacgg caaactcttt aaccgtagcg
     5941 aaagatattt atccgatgtt tgagcaggta ttaaaaaatc taccaagcac cattcaagga
     6001 gatattgttt acgataaaac cattgcgatt gatagttcta tcaaagaagt aattaaaact
     6061 attggtgagg caaccgtcat tgtattggta gtaattatcc tgttcttagg ttcattacga
     6121 gcaatgattg taccggtgat taccattccg atttccttaa tcggggtatt attcttcctg
     6181 caaatgtttg gtttctcgat caacttgctt actttgctgg cgttggtatt agcgatcggc
     6241 ttggtagtgg acgatgcgat tgtcgtactt gaaaacgtag aacgtcacgt taaagaaggt
     6301 aaaacgccat ttgatgcagc gattatcggt acacgtgaaa ttgctttacc ggttatttca
     6361 atgactatta ctctgggtgc agtatattca ccaatggcat taatgaccgg ggtaacaggg
     6421 acactattta aagagttcgc tttaacgctt gccggtgcgg tatttatttc ggggattgcg
     6481 gcattaaccc tttcgccgat gatgtctagc cgaatattaa aagaacataa agaaggggaa
     6541 gagtcagcat ttgcccgcgt ggtgcataga tcactagata aaatgaccag ctgctatacc
     6601 aatatgctaa gcggtattat ggctgttcgt ggtttcgtct tattctttgc ggtgctgatc
     6661 ttcgccagtt taccgatgtt atttggctca ctttccagtg aagttgcacc gacagaagac
     6721 cgtggctttg tggttggtat ttctaatgga ccttcaaaca ccaatttaga ttataccgaa
     6781 gcagcgttag cccaatttaa tcaggaagta actaaattac ctgaagtatc ttccttaatg
     6841 acagtttcag gctttaatgg tgcaaatagt gcattatcga ttattgcatt aaaagattgg
     6901 aatgatcgta gccgtgaacg tgctgcgatt tctaatgatg tggctgcaat cggtaaaaaa
     6961 gtggtgggta tggacgtgaa tgcaattgca ttccctgaaa tttcaaccgg tgaaaatggt
     7021 ctaccattct cattagtgat tactaccgct gatagctatg aaaaattggc aatcgttgcc
     7081 tctgaattct tgaaaaaagc ccaaacttcc ggtcagttct tctttgcgaa tttagatttg
     7141 aaatttgata cggtcacgat gaatatgaaa ttcgaccgtg aaaaaatggg tgcttatggc
     7201 gtgacaatgc agcaggtgag tggcacatta ggggcattct tatccggtgc gattattact
     7261 cgtgtcgata ttgactctcg tgcttacccg attgtgtcgc aagcggtgcg taatgatcgt
     7321 ttaaatcctg aagatttaat gaattactat gtcacgactg caacagggca atctatcccg
     7381 ttaggttcat ttgtgacgat ggaattaaaa acatcaccat cttctttacc tcgtatgagc
     7441 caattaaact cggccaccat tggtggggta gtatctggct caatcggcga tgcggtaaat
     7501 tgggcgaaag cagaattaga tcgcacttta ccatcaggtt atcaatatga cttccgtggt
     7561 gaatctcgcc agttcgttca agaaggtaat gcgatggcaa tgactttcgc tctagcagtt
     7621 atcattatct atcttgtact tgcaattcaa tttgaatctt ggcgtgaccc attagtaatt
     7681 ttggtttctg tgccgttagc tgtgagtggt gcattgttgg taatgaacat tccaaccgca
     7741 atgaaaggct tgtttatggg gattgcagca ggcactggct cgaaagatat gatggcattc
     7801 gccaacggta tttggacggc aggttatacc ttaaatatct attcacaagt aggtctaatt
     7861 acccttgtcg gcttaattac taaacacggt atcttgatgt gtgaagtggc gaaagaagag
     7921 cagttactgc acggcaaatc acgctatgaa gctattttcc acgcagccac gattcgttta
     7981 cgcccaatta tgatgacaac ggcagcaatg attgcaggct taattccgct tctgtttgcg
     8041 gtgggtgccg gtgcaatcgc ccgcttcagt atgggtatgg taatcgtagc aggcttggca
     8101 atcgggacat tgtttactct gtttatcttg ccggttatct acactttctt agggcaagaa
     8161 cacaagccat tacgtgaatt tgacgaggaa aaatatttcc caacgacaaa agaagcatag
     8221 ttttaaatag caaaagagcg tagcgatacg ctcttttttg ttggcttaca agcggtcgtt
     8281 ttttatttat tgtttgctaa aaaatcacaa aatttaaccg cttgaaagta tattttcaga
     8341 tagcgtaaac gtttccctat ttacagaaac ctagttcctc tgtataatcc gctcgcaatt
     8401 ttccagtaca gattttcaat ttaatcattt tttaaggaat aattgcaatg ctacgaatta
     8461 aacaagaagc cctcactttt gatgatgttc ttctcgtccc ggcacattct actgtgcttc
     8521 ctaatactgc tgatctttct actcaattaa ctaaaaccat tcgtttaaac attccgatgc
     8581 tttctgctgc aatggatacc gttacagaaa ctaagcttgc gatctccctt gctcaagaag
     8641 gcggcattgg ttttatccat aaaaatatgt cgattgaacg ccaggcagac cgtgtgcgta
     8701 aagtgaaaaa atttgaaagt ggtattgttt ctgagccagt gacgatttct cctgatatga
     8761 cattagcgga attggctgaa ttggtgaaaa agaacggttt tgcaggctat ccggtgattg
     8821 atgaaaacca aaatttagtg ggaattatta ccggacgtga tacccgattt gtcacggatt
     8881 taagcaaaac agtgcgtgaa tttatgacac caaaagaccg tttagtgacg gtaaaagaaa
     8941 acgcaagccg tgaagaaatt ttccacttaa tgcacgaaca ccgagtggag aaagtgctgg
     9001 tagtgaataa tgaatttcag ttaaaaggaa tgattaccct aaaagactac caaaaagcgg
     9061 aaagcaaacc gaatgcctgt aaagatgagt ttgggcgttt gcgtgtgggg gcggcagtgg
     9121 gagccggtcc gggcaatgaa gaacgaattg atgctttagt aaaagcgggt gtcgatgtgc
     9181 tattaatcga ctcttcgcac gggcattctg aaggtgtatt acaacgtgtg cgtgaaaccc
     9241 gtgcaaaata ccctgattta ccgattgttg ccggtaatat tgccactgca gaaggagcga
     9301 ttgcgttagc tgatgcagga gccagtgctg tgaaagtagg aatcggcccg ggttcaattt
     9361 gtaccaccag aattgtaaca ggcgttggcg tgccacaaat cacggcaatc gcagaagcgg
     9421 cagctgcgct taaagaacga ggcattcctg tgattgctga tggtggaatt cgttattcag
     9481 gcgatatttc aaaagctatt gccgccggtg caagttgcgt aatggtcggt tcgatgtttg
     9541 ccggcacaga agaagccccg ggtgaaattg agctttatca aggcagagca ttcaaatcct
     9601 accgtggaat gggatcatta ggtgcaatga gtaaaggctc gtcagatcgc tatttccaat
     9661 ctgataatgc cgccgacaag ctcgtaccgg aagggattga agggcgtatc gcttacaaag
     9721 gctacttgaa agaaattatc caccaacaaa tgggcggctt acgctcctgt atgggattaa
     9781 ccggctgtgc cactattgaa gaactccgca ccaaagcaga atttgtccgc attagtggtg
     9841 ctggtattaa agaaagccac gtccacgatg tgacaattac caaagaagca ccgaactacc
     9901 gaatgggtta atagcctttc tcactctctc cccttgtggg agagagaccg ttttaaatta
     9961 cgttcagtaa tttaaaacag agagagggaa taagtggtta gattttctta aaattttaca
    10021 aaagaggcta tatgctccaa gctatcattt ttgatatgga cggcgtgatt gtggataccg
    10081 aatatctgga attttcctta caaaaacagt ttattgaaga catcaaagaa cacagccgcc
    10141 cgattacttt agaacaacaa tcggaagtgg taggcaaatg tttaaaagaa attccggtga
    10201 ttatcaaaaa actaagcgaa tccagtttgc cgattgagga aattcgtgca cgttactacg
    10261 cctttttcca agatctgttt agcaaagtgg attttaaaac cattttcaga gccgatattc
    10321 agcaaattat tcaatttgcc aagcagaatc aaattaagct tgcggttgcc tcttcttccg
    10381 ccctttcgca tattgaaaat attctcacgg tttgcggcat taaagacgaa tttgatttaa
    10441 tcgtcagcgg tgaacagttt gaacacagca agccagaccc aaccatttac cgctacacct
    10501 gcgaaaaatt aggcgttgag ccacaaaatg cagtggcgat tgaagactcc tactacggaa
    10561 tgctcgccgc caaaaccgca ggcttaacgg tgattggtta cgaagaaaaa cgaatactga
    10621 ttgaccaaag tttagcggat tatatgggca aagatatgaa agagattttg aagattcttc
    10681 aaaaatgtaa tactccaagt taataaacgg tcgattttta tagaaatttt gtcaatgttt
    10741 ctacttcaag tacttggaac agtctatcgg cttgagtaac ctagaaatga gagtggatat
    10801 tttaatatta ataggataaa tagaatgaaa aaattaagtt taatttcaat cgtcgcttta
    10861 tctgcgtgca gtattttcgg accatcttat tcaggagaaa caacggccag tcctttatta
    10921 aagttggaca cagaaagaaa tataaatctg tattttcaag caatacataa atgtactcct
    10981 gataaaattc atacaaaaat catcaatatt ccaagtgaag gtaatgcaaa agaactttgg
    11041 actgcaacag gttgtaataa aacgcaatct tttattatta catatagaaa cgatggggta
    11101 ggcggcactt ttattaacat gaccacaacg ccataattta aaggaaatta caatgacaaa
    11161 catccaccac cacaaaattt taattttaga cttcggttca caatatactc agcttatcgc
    11221 acgccgtgtg cgggagattg gggtttactg cgagctttgg gcttgggacg taacggaaga
    11281 gcagatccgt gaatttaacc caacggggat tattctttca ggaggtcctg aaagcaccac
    11341 tgaggaaaac agcccacgag cgccggagta tgtgtttaac gctggtctgc cagtgctggg
    11401 catttgctac ggaatgcaga caatggcgat gcagttgggc ggtttaaccg aaacgtccga
    11461 ccaccgtgaa tttggctatg cctcggtggc tctcaaatct gccgatcaac tttttgcaaa
    11521 tttaaacgat gatttgaccg cttgcgagcc aaaattggac gtgtggatga gtcacggcga
    11581 caaagtcacc aaattaccac cgcactttac catcacaggc gttaccccaa cctgcccgat
    11641 tgccgcgatg tcggacgaaa gccgccgttt ctatggcgtg cagttccacc ccgaagtgac
    11701 ccacaccaaa caaggcttgg cgttgctgaa aaacttcgtg gtcggcattt gcggctgtga
    11761 aacgaactgg acggcagaaa acatcattga agatgcggtg aaacgcattc gtgagcaagt
    11821 gggcgatgac gaggtgattt taggtttgtc gggcggggtg gattcctccg tcaccgcctt
    11881 gctattacat cgtgctatcg gtaaaaacct gcactgcgta tttgtggata acggcttgct
    11941 ccgcttgaac gaaggcgatc aagtcatgga aatgttcggc aacaaattcg gtttgaacat
    12001 tgtgcgagtg aatgccgaag accgtttctt agaagcgtta aaaggcttag acgaaccgga
    12061 agctaaacgc aaaaccattg gtaaagtgtt tgtggacgtg tttgatgacg aaagcaaaaa
    12121 acttagctcg gtcaaatggc ttgcacaagg cacgatctac cctgatgtga ttgaatccgc
    12181 cgccagcaaa accggcaaag cccacgtgat taaatcacac cataacgttg gcggcttgcc
    12241 ggattatatg aagctcggct tagtcgaacc gttgcgtgaa ttgtttaaag acgaagtgcg
    12301 caaaatcggc ttggcactcg gcttaccggc tgaaatgctc aaccgccacc cgttccccgg
    12361 cccgggctta ggcgtgcgtg tgctgggcga aatcaaaaaa gagtactgcg atttactgcg
    12421 taaagccgat gcgatcttca tcgaagaact gcacaaagcc ggctggtact acaaagtcag
    12481 ccaagctttc acggtattct tgccggtgaa atcggtgggc gtaatgggcg atggacgcaa
    12541 atatgactgg gtcgtcagcc tgcgtgcggt ggaaaccatc gactttatga ccgctcactg
    12601 ggcgcacttg ccgtatgatc tattaggcaa aatctccaac cgcattatta acgaagtgaa
    12661 cggtatttcc cgtgtggtgt atgacgtgag cggaaaacca ccggcaacca ttgagtggga
    12721 ataactctta caaaataggc ttcttataac ctaagttcag tatctaaagc tgaaaaatgt
    12781 tgttaactgc ttaatgtagc ggtttagaat aaaggattga ttccaacgga atcaatcctt
    12841 aaactaagtg ccttttagta aaactaaata ttcaattttt agttttatga tttcagtaag
    12901 ccagatgaac cttcggaata atctctaggt tgatcttctt ccgttttttc tgccggaata
    12961 ggtaatttgg tttgttggaa aaaggtaata ttttttgatg cttctggtaa tagcttttca
    13021 gagctttgtg ctaaatgggt ataaagcttt ttataatcag aagctagact tgaaagtaat
    13081 tgggctgatt gctcaaagtg ttgttcaagt ttttgttttt gctcatccac ttgagttttg
    13141 gtttgcttca actctttttc cagttcatgc tgttttctga cactactgtt aaatactcta
    13201 actagagtgc aacctattac agcacctaca ataaatgctg ccataatggc tgcccaaaca
    13261 tctgttgtcc attgttccat cataaactcc ttatgtttta gtgattgatt actgctattt
    13321 ttaacatttc agtttttatt tgaaaagctc caaatgatta aaatgtgata taggttcaat
    13381 tttgaaagat tatcagcaaa aaatgttaga tttttgagtg aaatctctgt ataatttcgc
    13441 caccctgttt ttgtgtggtt ttccccgcca cacaagtttg gtctgaatca gactcgaagg
    13501 ggtcgggttc accgtaaaat aataggaaat acctcaatga ggtattgggt tttaaacttt
    13561 aaatttggta tttaattaat gaaaactttt gtagcaaaac cagaaacagt acaacgtgac
    13621 tggtatgtag tagatgcgac gggtaaaaca ttaggtcgtt tagcgactga attggcacgc
    13681 cgtcttcgtg gtaaacataa acctgagtac actccacacg ttgataccgg tgattacatc
    13741 atcgtgatca atgcagacaa agtagcagta acaggtaaaa aacaaactga caaaatctac
    13801 tactggcaca ctggctatgt aggtggcatc aaagatgcaa ccttcaaaga aatgattgct
    13861 cgtcgtcctg aagctgtgat tgaaatcgca gtgaaaggta tgttaccaaa aggtccactt
    13921 ggtcgtgaaa tgttccgtaa attaaaagtt tacgcaggtt cagaacacaa tcaccaagca
    13981 caacaaccac aagttttaga tatctaattc acggagcata aaaatgacag cagcaaatca
    14041 aaactacggc acaggtcgcc gcaaaagctc ttcagcccgt gtatttatca aaccgggttc
    14101 aggtaacatt actatcaacc aacgttcact tgaagtttac ttcggtcgtg aaacagctcg
    14161 tatgatcgta cgtcagccgt tagaattggt tgaattaact gataaattag acctttacat
    14221 cactgttaaa ggtggtggta tttctggtca agcgggtgca atccgtcacg gtatcactcg
    14281 tgcattaatg gaatacgatg aaacgctacg cccagcactt cgtgcagcag gcttcgttac
    14341 tcgtgacgca cgtcgcgttg aacgtaaaaa agtgggttta cacaaagcac gtcgtcgtcc
    14401 acaatactca aaacgttaag tgcgacaagt tgtcgtactt atggcaataa ctccttatat
    14461 atcaaggagt tattgctgga agtgggtggt attcttatca cctgcttccg actttccaag
    14521 cactcttcgg agtgcttgga aagacaggca agagaaaagg ggctttgccc aagcactctt
    14581 gttctaagtt gggaaagtaa atgaaccaac tcgaaaggct gtaaggtatg atcttcactt
    14641 tgatgtactt ggtttttttc ttaataccaa gatagagtaa gaaagcaaga tccgcagacc
    14701 ggagttaccc ataccccaga gcagagaaaa tttacaacac ctactataaa taaagtccaa
    14761 tagatcgcct cagcactcag aaaagacatc agaactaaga cttggtaaat cttagggaag
    14821 gagtgccatc tttacttata cttttcacac tcgcaatcta cggtaatcgt agaatgctcc
    14881 tcaatagtga ataaaaacac taaggataaa ctcttgttta ttcgttttta tgagctaggg
    14941 ataattatac cttaaattct cgttaaaatc acctctaata ctcacattag ctagggttag
    15001 ctaccttcgc ctattggaca ggttataggc tatttgcggg aaaatttgga tatagacaaa
    15061 gcgactttag tcgcataagt gaatttattt tgtaaaggtt cggacttatt tatgcagtat
    15121 aggatactga cttagtaagt atgcgtaagc tattactgtc attgttacgg tcaaacaatc
    15181 cgaagatatg agtagtacgg agggaatgat aaggtaacct gttatatggc taacagtagt
    15241 ctagggatag tgcgacaccg gtaacggtgt ggtgcggagc ttcctgacaa tgaacccctg
    15301 atgaaaatca gtcaatgaaa tcgtgagagg acattgggag aaagtgaata gcacaccttc
    15361 aaggggctag actgggtaga aagataaacg taagtgaaca gttgataaac ctcgttatcg
    15421 agagagagcc aaagctattg ataggctctg accaaaaggt aggcagtcgg ttattcttct
    15481 ttaacgtgag gatacgccat caaagcactg tcgggggaga gactgtatct tcactactcg
    15541 tgtgatagta atggaacttg gtaagcctgt atttctgcct tgcccacaat actcaaaccg
    15601 tttgaaatag atggcgaagg taaaagagga aaaggcaagt aaagcgtaag cgatactgtt
    15661 gggaatgcag gtaagggagg ttagaaaaag cgaatgctat gctgtaatgg catagatagg
    15721 gtgtgaaaca tagcctaacc cgaaagggtg cagacttcta actggtcttt catcgtaaga
    15781 tgaatagcgt ttcctttaag ggtaacaccg aaagagattg tagataacag aagagaaatt
    15841 gaatgttatc aaggaataca atgttgaata ggtacaaaaa caaattaaag aaatgagcgt
    15901 taggctcatc ataacctagc gctcgactct ttaacccaat cggttacaag agcggttgaa
    15961 attatcggta aaacggtaag atcctcttgt aaccaaaggg aataaactaa ggatagttta
    16021 tctcaatagg gatttgttta cttgaaggat ctacccactt gctgagactt tcgacttatc
    16081 ttgtaatatc agaggtaggt atgaaagagc gtaagcatta tactgaaaag ttagcttgca
    16141 atccagcgac ctcgcacatc tccaaatggc attatgtcga ttggcataaa gctgaaacgt
    16201 atgtgaaagg aatgcaaatt cggattgcga aggcaacact ggaaggcaat tggcgtaagg
    16261 ttaaaaactt acaacgaatg ctaacccact cacattatgc taaattagtt gcagtaagaa
    16321 gagtgactga aaatcaaggt aagcgaacaa gtggtgtcga taaagagctt tgggagaccc
    16381 cagagacaaa atggcaagcc gcactatcat tgacggaaaa agggtataaa cctagtccat
    16441 taagacgagt gtatatcccc aaaccgaatg gaaagaaacg tccgttgggt attccaacaa
    16501 tgcgagatag agcaatgcaa gccctttatt tattggcaat acaacctgtg gcagaaacaa
    16561 ctgcggataa aggttcttat ggatttagat taaaccgttc aacggtagat gctattactc
    16621 atatacacta aatttattcg cataaaggca agaaaactaa tcaacctgtt gagtgggtac
    16681 ttgatgcgga tattgcaggt tgttttgacc acataagcca cgactggcta ttaaagcata
    16741 ttccaatgaa taaacggata ttgcggaaat ggctaaaatc aggtgtagtg gaatttggac
    16801 ggttgaaacg aacagaggaa ggtacaccac agggtgggat tatctcgcca acgcttgcaa
    16861 atatggcatt agatggattg gaaaaactgt tagaaactca ttttggagga aaaggcagtc
    16921 ggacaattcg agaaaataaa acttacttag tacgttatgc agatgacttt atcatatcag
    16981 gtaaaacgaa agagttactt gaaaatcaag ttatcccact tatccagaac tttctgaatg
    17041 aacgaggttt atccttatca acagaaaaga cgaaagtggt acatattgaa gaaggttttg
    17101 attttctagg ttggaatgtg agacgtttca aaggaaaaat actgaacaga ccaagtcaaa
    17161 agaatgtgaa agcgttctac aacaaggtaa aagaggtaat ttctaaatga aaatggctaa
    17221 acaggaagat cttataatgg tgctaaaccc aatgttacga ggttgggcaa attatcatcg
    17281 aagccaagtt gcaagtaagg cattcggacg aatggacgca ttagtttgga aagtactttg
    17341 gaaatggtgt gaacgccgtc atcacaataa aggtattatg tggataaaag agaaatactt
    17401 ttgtaccaca gcgactcgaa attgggtatt tggcacagtt tataccaatg aaaaaggaga
    17461 aactaagccg atagacttat tctattgtgg ctatgttaaa atcaaacgac acgttaaaat
    17521 taagtctgaa tataatccgt ttttgcctga atgggagttg tacggcgaaa aactgagcca
    17581 agaacgtctg tatgaagaac aatcacacag acgacaatgg caggcactgt ataaagacca
    17641 gcgtggaaaa tgtgcattat gtgggttgcc aataaccaaa gaaacaggct ggcacgatca
    17701 tcatatcgtg tacaaaatgt taggaggaag cgatgcatta agcaatcgtt gtttggtaca
    17761 tccgacttgt cacataaaga ttcatacttt gaacttggag gtcgtgaagc cggctatata
    17821 gctgatgaaa ggcttgagct gtatgcagag aaatttgctt gtacagttct tagggggcgg
    17881 aggtgcagta atgtactttc gctacccgac ttttcttttc gttttcaaga aggcagggtt
    17941 tcctgccttt tttgtcttta tgactgtggt agaataaccg ccctgattaa ctatttgata
    18001 gaggcattta tgttagccat tatttcccct gccaaaaccc ttgattttaa aaccaatatt
    18061 gacggttttg cattttctca accaaatttg accgcttgta gccaagaatt aattgatatt
    18121 tgtaaacagc tttcacctgc tgaattaggt agtttgatgt caattagcga taaattagcc
    18181 gctttaaatg tggctcgctt tgccgagtgg cagcttgagc ataacgaaca aaatgccaaa
    18241 gccgccctct ttgcctttaa aggtgatgtt tataccggac tagaggcaga aacgttaagc
    18301 aaagcacaag ttgaatatgc tcaacaacat ttgcgtatgc tttcaggctt gtatggttta
    18361 ttaaagccgt tagatttaat gcagccttat cgtttagaaa tgggcactaa gttagccaat
    18421 ccgaaaggca aagatcttta tgctttctgg ggcaatatta ttactgaaaa tttgcagcaa
    18481 gcgattgatg aacaaggcga tgatatttta gttaatcttg cctctgatga atattatggt
    18541 gcggttaagc cacaaaatct aaatgcgact attatcaagc cggtgttttt agatgagaaa
    18601 aacggtaaat ataaagtgat cagcttctat gccaaaaaag cccgtggaat gatggttcgc
    18661 tttatattag acacccaacc gactagcatc gaacagctca aacagtttaa ctacgggggc
    18721 tattggtttg atgcagacag ttctagtgaa agcgagctag tctttaaacg tgaggagcag
    18781 gcataatgtc ggctatcaaa aagggaataa ctttggttgc gttatcattg gcaggttgtg
    18841 tcagccaaag cccacaattt gcagtgcctg atgctattca ctttgggcag gatacttttg
    18901 taaaagtcac ccacagccaa attgatgaaa tgcaacattt actttatttg ccagaaaaga
    18961 gtgagaaaaa tccggaaaat tggcaaaaag gcattttatt tttcttagat aaaaacagta
    19021 aaaatcaaac cttagagcaa cgagctgcat tccgccaagc aagttttgcg aaaaagccgg
    19081 agcttgaggc gaaagtggaa attcagcaaa atgaattaca cagcagtgtg atttatccac
    19141 ctatagaacg ttttaacaat gtgatgttag aagtaacccg tgggcggaat ttagcttgtg
    19201 gttacgggca gattcaattt gcagataagc gggaagttgg caagacagtt gcaaaaaaat
    19261 cgccgaattt gaccgcttac agccaagaaa ttgctagact ttctcttgaa ttaaaccaac
    19321 tgggttggca agtccaatgc agtaaataat gtagtaaatg aacgaaaaat acagcaaatt
    19381 agtcaaaaat gcagcgaatt tagcgattat tgttgcgatc ttactgattg tgattaaagc
    19441 ctttgcatgg tggaaaaccg gttcgatttc aatcttggca gcaatgacgg attcggtagt
    19501 ggatctgttt gcttcgctaa ccaatatttt agtgctgcgt tttgcactac aaccggcaga
    19561 tgagaatcac gcgtttgggc acggcaaagc ggagtctttg gcggcacttg cccaaagtgc
    19621 gtttatttcc ggctcggcgg ttttcctact gctacaaggt ttccataaac tcaccaatcc
    19681 ggaattaatt gctcaatcga gtattggggt aattgtcagt attgtatcaa ttgtgattac
    19741 tgcactttta gtcttttatc agcgttatgt tgtcacgctt acgaaaagtc cggcgattga
    19801 agcggattcg ttacattacc aaactgattt attaatgaat gcggcaattt tagtggcaat
    19861 gttgcttaat agtgccggtt ttgtgtacgc cgatgcagtt tttgcaatag gtattgccct
    19921 ctacattgct ttcaatgcag taaaaatgtt ttggtgtgcg gtgcaaacct tgctagacca
    19981 agctttgccc gatgaagaaa ttgagcggat taaagagatt gcttcgcaac atccgaacat
    20041 tttagggatt cacgatattc taacccgccg tgcaggtgca gtgcgtttta tccaattaca
    20101 tttggaatta gacgatcact taacgctgct agtagctcac gaaataactg atagccttga
    20161 gcagaaattg ctggcggcat ttccgatgtc tcacgtgatt attcatcaag agccgactac
    20221 ggtggtcaaa caagaatttc aagatatggc ggagaatatt taatgtatca ggcgatagtg
    20281 catttcagct atcataattt tgagcaagat ccggtgacgg caattagcca aatcttaaat
    20341 caatggttat ataacgggca agtcattggc agagaaatgc cgattacttt tcatcaaaac
    20401 gagtttcaag ctcgggtttg tgtgcctaca caagaaagcc ttttgccaca atataatagt
    20461 gatgaagtaa atgaagcgtt gcaacaagcg gtcgaaatcg gcgtaatttt tgcaagtttt
    20521 gagcttgtcg ggcgagatta tcagggcgaa gaaagcagtc ataaccgctc gccagcgttt
    20581 caaatattat ataccaccca tttagatacc tgctcgccac tttatgatgg agagcagttt
    20641 gccccaattc cgctttattc tttacaggat cagtctctta gcgaagcgat attggaatgg
    20701 cagcaaaatt ggcaagcgtg cgatctgcta caaatgaacg gcaatctgct ggagtgccac
    20761 gcacttgttc aaattagtga tgaaacaagc gatcttgcaa tgatcggtcg agatctatgt
    20821 cagcaaattg agcagaaaac tcacattccc accttttatt acctctaccg tttagggcgt
    20881 gatgaaagtg aatatcaacg caaatgccct tgttgtggcg gagaatggaa attaactgag
    20941 ccgttgcacg atattttcca ttttagatgt gagccttgcc gtttgatctc taatctaagc
    21001 tgggaattat tataactttt agttatacca aagtaataaa tcatcttgaa tgaatgccgc
    21061 aagcgtaata cccttgcggc atttttataa taagcggttt tactttggag gttctgtgct
    21121 tattttcaac ttaattgtct ttgcggcaat attattttta ttggctaaaa tttataaatc
    21181 tacgcaaaaa ctcggtcaaa cagtgtttat tgggttattt ttagggcttg caagcggtgc
    21241 ttttttgcaa actttttacg aaaaagctac tgttgatgca actcttgatt ggattaactt
    21301 aattggtaac ggttatgttc gcttgctaca aatgatcgta atgccattgg tgtttgtctc
    21361 tattttatcg gcaattaccc gcttaaaaga ggcaggatct ttaggcaaaa ttagttttag
    21421 tgtgctatca gtgttattag tgaccaccgc gattgccgca gcgattggta ttgctttagt
    21481 gtatctgttt gatttatctg ccgagggttt agtcgccggc tcccgtgagc ttgccgctca
    21541 agagaaagtg gtagggcgtg tggagcaggt gagcaactta tctgtgcctt caatgttgct
    21601 ttctttcatt ccgaaaaatc cgtttttaga attaaccggt gcaaacccaa cgtcgatcat
    21661 caacacggtt attttctcgg ctctgctagg cattgctgca ctgagcttag gcaaagaaga
    21721 taaagcgttg ggcgaacgta tcgcacaagg tgtagagacc ttaaataaac tggttatgcg
    21781 tttagtacgt tttgtgattc gcctaacccc ttatggcgta tttgcgttga tgatcaaaat
    21841 ggcggcaact tctcaatgga gcgatattgt aaatttaggt agctttattg tggcatcata
    21901 cattgcgatt ggtttgatgt ttattgtgca cggtatttta ctctttgtgg ctaaaatcaa
    21961 cccgttggat tactacaaaa aagtgttgcc gacattaagt tttgctttta cgtctcgttc
    22021 cagtgcggca accattccgt tgaatattga gacgcaaacc aataaattag gtaataacag
    22081 tgtgattgcc aacttttctg caacctttgg ggcaaccatc gggcaaaatg gctgtgcggg
    22141 aatttatccg gcaatgttgg cggtgatggt cgctccaaca gtgggaatgg atccgtttag
    22201 cttaaactat attttaacct tgattttggt ggtggcaatt tcctcatttg gcattgccgg
    22261 tgtaggtggt ggtgcaactt ttgcggcgat tgtggtgcta tcaaccttga atctgccgat
    22321 tgagttagta ggcttgctca tttcagttga gccgattatt gatatggctc gcaccgcctt
    22381 aaacgtgaat ggtgcaatgg tggcgggcac attaaccaat aaatgggtga aagccgagta
    22441 gtcaaaacta aagatataaa aatgggcgat taattcgccc gtttttataa gttaaggtga
    22501 ttattcaatc cacgcaatca cttcttcggt aggtttgcgg gattttttct taatttcttt
    22561 gttgcgatag ccaaaagtta tcatacaaga cacaccatat tcgtttaggt caaataagcc
    22621 ttcgtccgat aaaatttggt ttacggtatc gtaatgaaaa ccttcaatcg ggcaagagtc
    22681 aataccaatc agagccgcac cggtcatcat attgcctaat gcaatatagg tttgcttggt
    22741 actccaatcg aataattcac gttcactaag caatttcata tcctcttctt ggaacgactt
    22801 atagcgtacc agcgaggctt caatctgctc ataggttaag cctcgtttgg ctaacatatc
    22861 acgcatatag acagaatcat aacggacatt ctttttggtt aaaatcacca ctaaatggct
    22921 catttcttcc attgggtgtt taattcccca cgaaaccggg gcgattttct gacgggtttc
    22981 cgggttttgt agcactaaaa atttccacgg ttcagaaccg acagagcttg gtgagagacg
    23041 cccgagttcc aaaatataat ccatatcttc tttgttgatt tttttgtttg gatcgtaact
    23101 gcgacaggcc gaacggaagt gaaaagcgtc taaaacttgc tgtttgctaa tattaaacat
    23161 cgtttttcct tttataagtg aaataagtag attaatgata acttgattta gagcagaatg
    23221 cgaatttcac gcctgttaat ttgcaaaaaa ttaggaaaaa atgaccgctt gtttaaaatt
    23281 gctttacctt aaaaatcaat catttaacga atcggttgat ttactttttc tggctaaagt
    23341 attattattc atgaaatttt ttatttcatt gagtttagag gataggtaat gcaacaccta
    23401 aaagatttaa ctaataaagc acgtgaagct ttagataaga ttgatcaaaa tttagaggct
    23461 ttagaagagt ttagagtcga atattttggt aaaaaagggc attttacaac gttaatgcaa
    23521 gaattacgca atattgcagc ggaagaaaga ccggcaatcg gagcaaaaat taatgaagca
    23581 aagcaagttg tgcttgatgt gttaaatgcc aaaaaagaag aatgggaaca agctgtatta
    23641 aatgcaaaat tagctaatga gagcattgat gtttcactgc cggggcgtaa aactgaatta
    23701 ggtggtttgc accctgtttc tatcactatt gagcgtgtgg tgaaattttt ctctgaatta
    23761 ggttttaccg tagcaaacgg acctgaaatt gaaaccgatt actataactt cgatgcgtta
    23821 aacattccgg atcatcatcc ggctcgagcg gatcacgata cgttttggtt tgatgccaat
    23881 cgtttattaa gaacccaaac ctcgggtgtg caaatccgta caatggaaaa tgtaaaaccg
    23941 cctatccgta ttgtggcacc ggggcgtgta tatcgtaatg actacgacca aacccacaca
    24001 ccgatgtttc accaaatcga attgttatat gtggataaag aagcaaactt taccgagtta
    24061 aaaggcttaa ttcacgattt tctcaaagca ttctttgaag aagatttaca agtgcgtttc
    24121 cgcccgtcat tcttcccatt cactgagcct tctgcagaag tagatgtaat gcgtcaaaac
    24181 ggcaaatggt tagaagtgtt aggttgcggt atggtacacc cgaatgtgtt acgcaatgta
    24241 gggattgacc ctgaagaata tagcggtttt gctgtgggaa tgggcgtcga gcgtttaacg
    24301 atgttacgct acaatgtcac ggatttacgt tcattctttg aaaatgactt acgtttctta
    24361 aaacaattta agtaatacaa gcggttaaaa atttagaaaa ttttacaaat aggatagaaa
    24421 taatgaaatt caatgaatct tggttgcgtg aatgggtaaa cccagcaatt agcacagaac
    24481 agttatgcga ccaaattaca atgttagggt tagaagttga cgatgtagag ccggtagctg
    24541 gtgcgttttc gggtgtagtt gtgggtgaag tggtggaatg cgcccagcac ccggatgctg
    24601 acaaattgcg tgtcactaaa gtgaatgttg gtggcgaacg tttattagat attgtttgtg
    24661 gtgcaccaaa ctgccgtcaa ggtttaaaag tggcttgcgc tgttgaaggt gcagtgctac
    24721 cgggcgattt caaaattaag aaaaccaaat tgcgtggtca gccttcagag gggatgttat
    24781 gttcttactc agaacttggg attaaagaag atcatagcgg tattattgaa ttacctgctg
    24841 atgcacctat tggtcaagat ttccgtgaat atttaaattt aaatgatgtg gcgattgaaa
    24901 tcagccttac gccaaaccgt gccgactgct taagcattgc cggcattgct cgtgaagtgg
    24961 gggtgattaa ccgtgtcgat gtgaaagctc caactattac agatgtaaaa gccacgattt
    25021 cagataaagt ttcagtggaa ttacaagcac cggaggcttg ccctcgctat ttagcccgtg
    25081 tggtgaaaaa tgtgaatgtc aaagcgacat cgccactttg gttacaagaa aaattacgcc
    25141 gttgtgggat tcgctcaatc gatccaatcg ttgatattac taacttgagc cttttagaat
    25201 tagggcagcc aatgcacgcc tttgatgcag cgaaaattga aggtggcatt caagtgcgta
    25261 tggcaaaaga aggcgaagaa ttggtattgc tagatggcac aaacgctaaa ttacaatcga
    25321 ataccttagt gattgctgat agcaaagggg cattggctat ggcaggtatt ttcggtggcg
    25381 aagccagcgg ggtgagcgag aatacgaaag atgtggtgtt agagtctgca ttttttgctc
    25441 cattagcgat taccggtcgc gctcgtcaat atgggttaca taccgacgca tcacaccgtt
    25501 ttgagcgtgg ggttgatcca gaattaactc gtcaagcaat ggagcgtgca acggcattat
    25561 tactggaaat ttgcggcggt gaagcaggtg aaattgtgga agccgtgagc caagaaaacc
    25621 taccaaaacg caatactgtc acgcttagac gcagtaaatt agattcggtg attggtcatc
    25681 atattgacga tgcaacggta accgatattt taacccgctt aggcttgaac gtaacatttg
    25741 cggataatag ttggacggca attgctccaa gttggcgttt tgatattgaa attgaagaag
    25801 atttaattga agaaatcgcg cgaatttatg gctacaacag tatcccaaat aattcgccac
    25861 ttgctcattt aacgatgaaa ggtacgccgg aaaaattact ggaagcaaac cgtattcgta
    25921 tggcattggt agatagtgat taccaagaag tgattactta tagctttgtt gatccgaaaa
    25981 aacaatcatt attgcaccca cagcaagaag ccttgatttt accaaacccg atttcaagtg
    26041 aaatgtcggc aatgcgtgtt tctcttttaa ccggtttgtt ggacactatc gtgtataacc
    26101 aaagccgtca gcaaaaccgt gtgcgtattt ttgaagcagg tctacgtttt attccggata
    26161 gcaatgcgga atcaggcgtg cgtcaagaat ttgtttttgg tgcggcgatt gtaggcgata
    26221 aacgtccggt acattgggaa aacaaatcag aagcagccga tttctttgat ttaaaaggcg
    26281 atattgagcg tattctttct ttaacctcta tccgccacga tctgcaattt gtcgcaaaac
    26341 agttcccagc attacacccg gggcaatcgg ctgcgattat gcttgatggc aaagaaattg
    26401 gttttatcgg aactgttcac ccgtcaattg tgcaaaagtt tggcattaaa ggcaaaccga
    26461 ttgtatgtga aattctagca tcagctattg cagagcgtcc ggttacacaa gcgggcgaaa
    26521 tttctaaatt tcctgcaaat aaccgtgata ttgcggtagt ggttgatgaa tctgttcctg
    26581 caggtgaggt gttagaggct tgtcgtaaag ctggtggtaa taaattagtg gcagtaaatt
    26641 tatttgacgt ttaccgtggt tcgaatttag aaagtggtaa gaagagttta gccattagct
    26701 taacggttca agataatgaa aaaacattag aagaaaatga aatcaatgcg gtaattcaag
    26761 cggtattagc tgaattagca acacgtttca atgcggtact tcgtgattaa tcagtaaaat
    26821 atggctgatt tattttatca gccataacaa taaactaagg ataaatgatg gcgcttacca
    26881 agattgaaat tgcagaaaac ctgattgaaa aatttgggtt agaaaagcgt gtagcgaagc
    26941 aatttgttga attgttcttt gaagagatcc gatcctccct cgaaaatggt gaagaagtga
    27001 aattatctgg ttttggtaat ttttcactga gagaaaaaaa agctcgtcca ggaagaaatc
    27061 ctaaaacggg ggaaaatgtg gctgtttcag cccgtcgagt agtagttttt aaagctgggc
    27121 aaaaattacg agaacgtgtt gagcaagcta gctcccaatc ataggggcag ttatattagt
    27181 tagatttaat gtggagtaat aaatgaaatt aaccaaacgg tggctttcct taggttgttt
    27241 gttagctatt acagcgatgt tagtggcttg ttcaagttca gatacaacct ctaagtctca
    27301 agtaaaaagt aaagtttatc gttcaatata ccaatcagac ctttctgatc ctattatggc
    27361 aattagtcga ttaagtgaac atcaacacga atggaaaggc actcgctatc gtttaggtgg
    27421 caatagcaag gctggcatcg actgttctgg ttttatgcaa gtcactttta aagatctttt
    27481 tgggatagat ttgccgagaa ccaccacaga gcaggctaag gctggtgagc gtatatccaa
    27541 agacgaattg cgtacaggtg atttagtttt tttcaagaca gggcgtggtc caaatgggaa
    27601 acacgttggt gtttatgtaa aaaatgggca atttcttcac gcctcaacaa agggaggggt
    27661 gatttattct gatttggatt cgccatattg gtctaaggct ttctggcagg ctcgtcgttt
    27721 ataatttagg gtaacataca tgctgaaaaa aatcggacaa gcggtcatat ttggactttt
    27781 ttgtgcaatt ttaattttgt ttgtagctcc aattattcag agtaaaggga gttcgttttc
    27841 attatcagca ttcactcaaa aagagattgt aagctacaat gcagcggtta aaatagcctc
    27901 ccccgcagtc gttaacgtat atagccgaaa ttttagttct gaaaatgaaa catttgaagt
    27961 taagaattta ggttcgggtg tgattatgag tagcgatggc tatattttaa ctaacaggca
    28021 tgtgattgaa gatgcggatc aaatcgtgat tgctttgcaa aacggtgcta tttcgactaa
    28081 tgtaaaatta gtcggcgatg attctttaac tgatttagct gttttaaaaa ttgagggtga
    28141 gaatttacct actattccac aaaatccgga gcgcaaacta caaattggcg atgtggtgtt
    28201 ggcaattggt aatcctttga atttaggaca gagcattacg caagggatag tcagtgctat
    28261 tgggcgaaaa accttaacag aaagtggtag acaaaatttt attcagacag atgtttccat
    28321 taatcaaggg aactcaggtg gtgctttaat taatactgct ggtgagctaa taggtattaa
    28381 cactttgcgt tttgggaaaa atacgaatga attgtcagag ggcctaaatt ttgcgattcc
    28441 cattaatctg gcgaatcaaa ttatgagtaa gatcattaaa gatggccgag ttattcgtgg
    28501 ctattttggg gtaagtacag atttgcttga caccgcaaaa atgcaaggtt atgaaaaagg
    28561 ggtgatcatt gttaatgtag ataaaaatgg gccagccgca aaagcgggga ttcaagcagg
    28621 tgacttagtg cttaaagtcg gagatattga ggcattctct ccttctcaaa tgatggaaga
    28681 gcttgctgaa atgaaacctt atactacagt aaaagtattg gttcaacgtg atgaacaatt
    28741 acttaacttt gatgtaacta ttactgagtt acagactcaa tagggtagaa aatgaaaaaa
    28801 atagtgatta cagttgatgg gccgagtggt gcaggtaaag gaacgttatg tcatgcattg
    28861 gctgaaaaat tagggtttga ttttttagat tctggagcct tataccggat tactgcatta
    28921 agcgcaatta aaaaagaggt tccccttgat gatgaggaaa aactagcgga agttgggcgt
    28981 aatcttgata ttcaattttt gccagaaaat ggcgagattc aggtgatttt ggaaggagaa
    29041 aatgtaggcg acttaatccg aacggcagaa gcaggtcaaa atgcctctaa agtcgctgct
    29101 ttccctttgg tacgtgaagc acttttagaa cgccaaagag cctttagtag cgaaaaaggt
    29161 ctgattgcag atggacgaga tatggggaca gttgtgtttc ctgatgcaca aattaagctg
    29221 tttttagatg caagtgccga agaacgcaca aaaagacgtg taaaacagtt gcaagaaaag
    29281 ggatttaatg ctaactttga cgagatttta gccgagataa aagaacgtga ttttcgcgat
    29341 agaaatcgat cagtcgcgcc acttgttccg gctaaagatg cgttgctgtt agattcaacg
    29401 catttatcaa ttgaagatgt gattaaccaa gcattaaatc atatcgctca atacattaaa
    29461 ttttagtttg ttgcttcaca taggatttgt gtagctttta ttatcaaccc cgtttaacag
    29521 gacgttagat ggatgtttat ttttaagaag atttattatg tcagaattat cgtttgctca
    29581 actacttgaa gaatcatttg cagctacacc acgtttaggt gatgttgtta aaggtactgt
    29641 tgttgctatc caaaaaggct tagttattgt tgatacaggt ttcaaatctg aatctgcaat
    29701 cgatgcctca gaatttacta acgcacaagg tgaattagaa gttcaagtgg gtgatgaagt
    29761 agatgttgtt cttaaagctg tagaagacgg ttttggtgca acagtagttt ctcgtggtga
    29821 tgcaaaacgt aacgaagctt ggattgcttt agagaaagca tttgaagaac aagctacagt
    29881 tatcggtttc gtaaatggta aagtaaaagg cggtttcact gttgaattaa acggtgttcg
    29941 tgcattctta cctggttcat tagttgatac tcgtccggtt cgtgattctt taaacctaga
    30001 aggtaaagag tacgaattca aagtaatcaa attagatcaa aaacgtaaca acgtagtagt
    30061 atctcgtcgt gctgttatcg aaacagaaag caaccaagat cgtgatgcgg tattagcaaa
    30121 cttagttgaa ggttctgaag ttaaaggtac agttaaaaac ttaactgact acggtgcatt
    30181 cgtagattta ggtggcgttg acggtttatt acatatcaca gatatggctt ggaaacgtgt
    30241 taaacaccca agtgaagtgg ttaatgtagg cgatgaagtt actgttaaag tattaaaatt
    30301 tgacaaagac aaaactcgtg tatcattagg cttaaaacaa ttaggtcaag atccttgggc
    30361 tgtaattgct gaaaaccacc cggtaaacag caaattaact ggtaaagtaa ctaacttaac
    30421 tgactacggt tgcttcgttg agattttaga cggtgttgaa ggtttagttc acgtttcaga
    30481 aatggattgg acaaacaaaa acattcaccc atctaaagtg gttaacgtag gcgatgtagt
    30541 tgaagtaatg gtacttgaag ttgatgaaga acgtcgtcgt atctcattag gtttaaaaca
    30601 atgcaaagca aacccttggg aacaatttgc ggcaactcac agcaaaaatg ataaagtatc
    30661 aggtaaaatc aaatcaatta ctgacttcgg tattttcatc ggtttagaag gtggtattga
    30721 cggtttagtt cacttatctg acatttcttg gaatgtttca ggtgaagaag cagttcgtaa
    30781 ctacaaaaaa ggtgatgaag tagaagctgt tgttctacaa gttgatgcag tgaaagagcg
    30841 tatctcttta ggtatcaaac aattagaatc tgatcctttc acaaactttg tagatggtac
    30901 taaaaaaggt gcaatcttaa caggtaaagt tgtagaagtt gatgctaaag gcgttaaagt
    30961 tgaattggaa ggtggcgttg aagcatttat tcgtgcaaac gaagcaactg ctgagcgtgt
    31021 agaagacatc acttctgtta tttctgttgg cgattcaatc gaagcgaaat acaccggtgt
    31081 tgatcgtaaa tctcgcgtag ttaacttatc tgttcgtgca aaaaacgaag ctgaagaatc
    31141 agcagcaatt gcacaagtga acaaagagga agttgttgta cctaatgcat ttgctgaagc
    31201 tttcaaagca gctaaaggcg aataattagt atagactaat ctaaa
//
LOCUS       DANLRK010000008        30222 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.008, whole genome shotgun sequence.
ACCESSION   DANLRK010000008 DANLRK010000000
VERSION     DANLRK010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 30222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30222
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.008"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            105..608
                     /locus_tag="PXA33_000250"
     CDS             105..608
                     /locus_tag="PXA33_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-activating lysine-acyltransferase"
                     /protein_id="HDL5119630.1"
                     /translation="MNQSYFNLLGNITWLWMNSSLHKEWSCELLARNVIPAIENEQYM
                     LLIDNGIPIAYCSWADLNLETEVKYIKDINSLTPEEWQSGDRRWIIDWVAPFGHSQLL
                     YKKMCQKYPDMIVRSIRFYPKQKELGKIAYFKGGKLDKKTAKKRFDTYQEELATALKN
                     EFNFIKK"
     gene            624..3485
                     /locus_tag="PXA33_000251"
     CDS             624..3485
                     /locus_tag="PXA33_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RTX family hemolysin"
                     /protein_id="HDL5119631.1"
                     /translation="MGTRLTTLSNGLKNTLTATKSGLHKAGQSLTQAGSSLKTGAKKI
                     ILYIPQNYQYDTEQGNGLQDLVKAAEELGIEVQREERNNIATAQTSLGTIQTAIGLTE
                     RGIVLSAPQIDKLLQKTKAGQALGSAESIVQNANKAKTVLSGIQSILGSVLAGMDLDE
                     ALQNNSNQHALAKAGLELTNSLIENIANSVKTLDEFGEQISQFGSKLQNIKGLGTLGD
                     KLKNIGGLDKAGLGLDVISGLLSGATAALVLADKNASTAKKVGAGFELANQVVGNITK
                     AVSSYILAQRVAAGLSSTGPVAALIASTVSLAISPLAFAGIADKFNHAKSLESYAERF
                     KKLGYDGDNLLAEYQRGTGTIDASVTAINTALAAIAGGVSAAAAGSVIASPIALLVSG
                     ITGVISTILQYSKQAMFEHVANKIHNKIVEWEKNNHGKNYFENGYDARYLANLQDNMK
                     FLLNLNKELQAERVIAITQQQWDNNIGDLAGISRLGEKVLSGKAYVDAFEEGKHIKAD
                     KLVQLDSANGIIDVSNSGKAKTQHILFRTPLLTPGTEHRERVQTGKYEYITKLNINRV
                     DSWKITDGAASSTFDLTNVVQRIGIELDNAGNVTKTKETKIIAKLGEGDDNVFVGSGT
                     TEIDGGEGYDRVHYSRGNYGALTIDATKETEQGSYTVNRFVETGKALHEVTSTHTALV
                     GNREEKIEYRHSNNQHHAGYYTKDTLKAVEEIIGTSHNDIFKGSKFNDAFNGGDGVDT
                     IDGNDGNDRLFGGKGDDILDGGNGDDFIDGGKGNDLLHGGKGDDIFVHRKGDGNDIIT
                     DSDGNDKLSFSDSNLKDLTFEKVKHNLVITNSKKEKVTIQNWFREADFAKEVPNYKAT
                     KDEKIEEIIGQNGERITSKQVDDLIAKGNGKITQDELSKVVDNYELLKHSKNVTNSLD
                     KLISSVSAFTSSNDSRNVLVAPTSMLDQSLSSLQFARAA"
     gene            3560..5686
                     /locus_tag="PXA33_000252"
     CDS             3560..5686
                     /locus_tag="PXA33_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_063101322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HDL5119632.1"
                     /translation="MEANHQRNDLGLVALTMLAQYHNISLNPEEIKHKFDLDGKGLSL
                     TAWLLAAKSLALKAKHIKKEISRLHLVNLPALVWQDNGKHFLLVKVDTDNNRYLTYNL
                     EQDAPQILSQDEFEACYQGQLILVTSRASVVGQLAKFDFTWFIPAVIKYRKIFLETLI
                     VSIFLQIFALITPLFFQVVMDKVLVHRGFSTLNIITVALAIVIIFEIVLSGLRTYVFS
                     HSTSRIDVELGAKLFRHLLSLPISYFENRRVGDTVARVRELDQIRNFLTGQALTSVLD
                     LLFSFIFFAVMWYYSPKLTLVILGSLPCYILWSIFISPILRRRLDEKFARSADNQAFL
                     VESVTAINMIKAMAVAPQMTDTWDKQLASYVSSSFRVTVLATIGQQGVQLIQKTVMVI
                     NLWLGAHLVISGDLSIGQLIAFNMLSGQVIAPVIRLAQLWQDFQQVGISVTRLGDVLN
                     SPTEQYQGKLSLPEIKGDISFKNIRFRYKPDAPTILNNVNLEIRQGEVIGIVGRSGSG
                     KSTLTKLLQRFYIPENGQVLIDGHDLALADPNWLRRQIGVVLQDNVLLNRSIRENIAL
                     SDPGMPMERVIYAAKLAGAHDFISELREGYNTIVGEQGAGLSGGQRQRIAIARALVNN
                     PKILIFDEATSALDYESEHIIMQNMQKICQGRTVILIAHRLSTVKNADRIIVMEKGEI
                     VEQGKHHELLQNSNGLYSYLHQLQLN"
     gene            5698..7134
                     /locus_tag="PXA33_000253"
     CDS             5698..7134
                     /locus_tag="PXA33_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_140441352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HDL5119633.1"
                     /translation="MKIWLSGIYEFFLRYKNIWAEVWKIRKELDHPNRKKDESEFLPA
                     HLELIETPVSKKPRLIAYLIMLFLVVAIVLASVSKVEIVATAPGKLTFSGRSKEIKPI
                     ENAIVQEIFVKDGQFVEKGQLLVSLTALGSDADIKKTMASLSLAKLENYRYQTLLTAI
                     EKESLPVIDLSRTEFKDSSEEDRLRIKHLIEEQYTTWQKQKTQKTLAYKRKEAEKQTI
                     FAYVRKYEGATRIEQEKLKDFKALYKQKSLSKHELLAQENKLIEAQNELAVYRSKLNE
                     LENDLLNVKEELELITQFFKSDVLEKLKQHIENERQLRLELEKNNQRRQASMIRAPVS
                     GTVQQLKIHTIGGVVTTAETLMIIVPEDDVLEATALVPNKDIGFVAAGQEVIIKVETF
                     PYTRYGYLTGRIKHISPDAIEQPNVGLVFNATIAIDRKNLTSPDGRKIDLSSGMTITA
                     EIKTGERSVMSYLLSPLEESVTESLRER"
     gene            7375..8373
                     /locus_tag="PXA33_000254"
                     /pseudo
     CDS             7375..8373
                     /locus_tag="PXA33_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250818.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            8390..9133
                     /locus_tag="PXA33_000255"
     CDS             8390..9133
                     /locus_tag="PXA33_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL5119634.1"
                     /translation="MKQKYIYGLAGLLLLLLGWQLGTALLSQRLPMASMLAPAKTWES
                     FTWLISSGEIFPHLWASLQRIAVGLLAALVIGVPLGLLLGFSKHLEMLCTPSFQLLRM
                     ISPLSWMPIIVMLLGVGDLPIYFLLAFATVWSLIISTTSGVKSIDPQWLQLGRVMSAT
                     RKELLCKIVVPAITGHVLTGLRVAVGIAWVVLVPCEMLGVNQGLGYFILDTRDRLAYS
                     ELMAVIIIIGLIGWALDSLIRLALAKGGR"
     gene            9288..10346
                     /locus_tag="PXA33_000256"
     CDS             9288..10346
                     /locus_tag="PXA33_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HDL5119635.1"
                     /translation="MSVLSSSFIQWLQQNAENLDQSNERADLLIPQIAALGLYKIGVP
                     ETLGGSGGTLVDAIKAVTELGNHSLAAAFVSWGHRTFIEHIIASKNPIPRQSYLADLL
                     NGNLTAGTGLSNAMKFLSGIEELNVNIREENGKRYLNGRLPWVTNLRSYRFVASFVAG
                     FQDERKPLVIAIPSTAKGLTRTKELEFIALQGTNTTSLVFENVELQDEWILADDAPEF
                     LAATRPAFLGLQFGLPFGLVERSLAEIEKSLPFRAILASEWQAQVDALNDIKTRLFNG
                     LNEEGYFVRNPKELFQARIDIVNVVAQTILLELQASGGRGYLKNDTSGFSRRWKEAAF
                     LPVVTPSAVQLRMVLANS"
     gene            complement(10467..11009)
                     /locus_tag="PXA33_000257"
     CDS             complement(10467..11009)
                     /locus_tag="PXA33_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HDL5119636.1"
                     /translation="MSQFQIHTIESAPETAQEALKAVQQANGFIPNLIGVLANAPTAL
                     ETYRTVGGINSRNSLTAEEREVVQITAAVVNGCGFCVAGHTKIALKALKMPEEVVNAL
                     RATARIDDNKLDTLARFTLAVMLQKAKLTEAQLNEFFAAGYNQQNAIDVILGVSLATL
                     CNYVNNIAEIPINPELQPFA"
     gene            complement(11161..12672)
                     /gene="glpK"
                     /locus_tag="PXA33_000258"
     CDS             complement(11161..12672)
                     /gene="glpK"
                     /locus_tag="PXA33_000258"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HDL5119637.1"
                     /translation="MEKQYIIALDQGTTSSRAVLLDKNANVVEVAQREFTQIYPQAGW
                     VEHNPMEIWSSQSSTLNEVVAKAGIKPDSIAAIGITNQRETTIVWEKETGKPVYNAIV
                     WQCRRTSDITDKLKADGHEEYIRKTTGLVVDPYFSGTKVKWILDNVEGAREKAERGEL
                     LFGTVDTWLVWKLTQGRVHVTDYTNASRTMLFNIHTKQWDDKMLELLNIPRSMLPEVK
                     NSSEVYGQTNIGGQGGVRIPVAGIAGDQQAALYGHLCVKEGQAKNTYGTGCFMLMHTG
                     NTAIQSENGLLTTIACNAKGEPEYALEGSVFIAGASIQWLRDELKIVHDSFDSEYFAT
                     KEDDSNGVYVVPAFTGLGAPYWDPYARGSIFGLTRGANRNHIVRATLESIAYQTRDVL
                     EAMQSDSKAKLQALRVDGGATANNFLMQFQADILDTKVERPKVKEVTALGAAYLAGIA
                     VGFWKDLEELKDKAEIERTFTPDGDQEKRAKRYKGWKKAVRRSLEWAKEDAEE"
     gene            complement(12676..13461)
                     /locus_tag="PXA33_000259"
     CDS             complement(12676..13461)
                     /locus_tag="PXA33_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HDL5119638.1"
                     /translation="MSKSLRNACIGEFIGTGFILFFGAGCVAAAQVAGANFGLWEISI
                     VWGLGVSMAIYISAGISGAHLNPAVTIALAAFYGFEKHKILPYVIAQVAGAFCSVALI
                     YFMYSDLFTAAEAAQGITRGETVGFAGVFSTYPNPNITLVTAFIVEFVITVVLMSTIL
                     AIGDDKNGLPNKALAALLIGLLIAVIGGATGPLTGFAMNPARDFGPKLFAYLAGWGEI
                     ALTGGKEIPYFIIPIVAPICGALFGAWGYKNLIHNNLPANTQE"
     gene            13674..14033
                     /locus_tag="PXA33_000260"
     CDS             13674..14033
                     /locus_tag="PXA33_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HDL5119639.1"
                     /translation="MISIGAVLGAMCRWQLAVWLNPILSQFAFGTLAANWLGCLLIGI
                     AMGFNLGESQRLLFISGFLGSFTTFSSFSLELTEKLLAEKWGQFVTVLSLHLIGGLLL
                     TLIGVMLARAVTSGRFC"
     gene            complement(14081..15403)
                     /locus_tag="PXA33_000261"
     CDS             complement(14081..15403)
                     /locus_tag="PXA33_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HDL5119640.1"
                     /translation="MSAMFIIQFAIVLLCILVGARVGGIGLGVFGGLGLAILSFGFGL
                     KPAGLPIDVMFMIMAVVSAAAAMQAAGGLDYMIKIATRILRKNPKYITFIAPLVTWTF
                     TVLAGTGHVAYSVLPVIAEVSRHNGIRPERPLSMAVIASQFAIVASPIAAAVVAVVAF
                     LEPQGVTLGNVLSVTIPATLLGLGLACVFVNKMGKELKDDPNYQRLLQDPEYVKENHI
                     TTNPTELPLKPTAKLSVGLFLFGALLVVLMGAIPALRPVFDGKPMGMAHTIEIVMLSI
                     GALIIFFCKPDGNEITKGSVFHAGMRAVIAIFGIAWLGDTLMQGHMDEVKSMVSGLVE
                     TAPWAFAFALFVLSVLVNSQGATVATLFPVAIAIGIPAPVLIGVFVAVNGYFFIPNYG
                     PIIASIDFDTTGTTRIGKYIFNHSFMLPGLLSMIFSIGFGLLLSNILL"
     gene            15552..16376
                     /gene="dapF"
                     /locus_tag="PXA33_000262"
     CDS             15552..16376
                     /gene="dapF"
                     /locus_tag="PXA33_000262"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HDL5119641.1"
                     /translation="MQFSKMHGLGNDFMVVDGVTQNVYLTEEMIRTLSDRHRGIGFDQ
                     LLLVEPPYDPELDFHYRIFNADGSEVSQCGNGARCFARFVTLKGLTNKKDIHVSTAKG
                     KMVLSLQEDGKVRVNMGEPIWEPSQVPFTANKFEKNYILRTELQTVLCGVVSMGNPHC
                     VLQVDDVKTAPVNELGPLLESHERFPERANISFMQVVNRNHVKLRVYERGAGETQACG
                     SGACGTVAVGIMQGLLDNKVQVDLPGGTLIIEWQGVGHPLYMTGDATHIYDGFIKL"
     gene            complement(16547..17089)
                     /locus_tag="PXA33_000263"
     CDS             complement(16547..17089)
                     /locus_tag="PXA33_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase family protein"
                     /protein_id="HDL5119642.1"
                     /translation="MAQVYIVHGYTANADKHWFPWLEQELAKCGVSCERLNMPDSAKP
                     SLDGWLNHLAQNVELTDQTVFVGHSLGCIAILNFLAKNYAKIKGAVFVSGFYQPVENL
                     PELSAFTNYYAILPSVSSFPSYVVSALDDKVVNHQYSDALAQHLHADYIRLNQGGHFL
                     DREGVTELPVVLELVKKLLE"
     gene            17213..17536
                     /locus_tag="PXA33_000264"
     CDS             17213..17536
                     /locus_tag="PXA33_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QacE family quaternary ammonium compound efflux
                     SMR transporter"
                     /protein_id="HDL5119643.1"
                     /translation="MNVWILLAISICLEIAATNLLKLSNGFTKAIPTIGSLALYGLSF
                     YFLSIIFRTLPVGIVYAIWSGVGIVLTALVAYFAFGQKIDLAGLIGISLIIAGVLVIN
                     LFSKV"
     gene            complement(17599..19044)
                     /gene="xylB"
                     /locus_tag="PXA33_000265"
     CDS             complement(17599..19044)
                     /gene="xylB"
                     /locus_tag="PXA33_000265"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HDL5119644.1"
                     /translation="MYIGIDLGTSGVKVVLLDENQHIIAITQKSLPIFRPHPLWSEQN
                     PQDWWNATNEAMLELSATQDLQAVKAIGLTGQMHGATLLDNADNVLSPAILWNDGRSF
                     MECEELEQLVPNSREITGNLMMPGFTAPKLRWVDKHQPDIAEKVSKVLLPKDYLRLLM
                     SGEYASDMSDASGTMWLDVGKRNWNKSLLNACGLDIENMPKLFEGNQITGYLRPTLAE
                     QWKMKSVPIVAGGGDNAAGAIGIGLYQTGQAMLSLGTSGVYFVVSDKFQANPQKAVHS
                     FCHALPDRWHLMSVILSAASAVDWAVKSLGLKDVSTLFQQVEASQTASDAIFLPYLSG
                     ERTPHNDPYAKGVFWGLSHNDNQITMAKAVIEGVSFALAEGIEVLHETGVVADNIALI
                     GGGAKSVYWRQLLADISGKTFEYRTSGDVGPALGAAKLAQIALNPNQDIATFCQPLPL
                     EQVYQPTPARYAEYQQKRQKFTELYRRLKGL"
     gene            complement(19197..20516)
                     /gene="xylA"
                     /locus_tag="PXA33_000266"
     CDS             complement(19197..20516)
                     /gene="xylA"
                     /locus_tag="PXA33_000266"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012072255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HDL5119645.1"
                     /translation="MSNYFDKISKVQYEGANSTNPFAFKHYNPDEVVLGKTMAEHLRL
                     AVCYWHTFCWTGNDMFGVGSLDRSWQKTGDLLQGAKQKAEIAFEFFQKLGIPYYCFHD
                     VDVAPEGNNFKEYQHNFNTIVDILEQKQAETGVKLLWGTANCFTNPRYMSGASTNPNP
                     EVFAWAAAQVFTAMNATKRLGGENYVLWGGREGYETLLNTDLKREREQIGRFMQLVVE
                     HKHKIGFNGTLLIEPKPQEPTKHQYDYDVATVYGFLKQFGLEKEIKVNIEANHATLAG
                     HTFQHEIATATALDIFGSIDANRGDPQLGWDTDQFPNSVEENTLVMYEILKAGGFTTG
                     GFNFDAKIRRQSTDPYDLFHAHIGAIDVLALSLKRAAKMLEDQALQKVVDNRYAGWNQ
                     DLGLQILNGKASLEDLAKIVETQGLDPKPVSGQQEYLENLVNSYIYR"
     gene            20758..21759
                     /gene="xylF"
                     /locus_tag="PXA33_000267"
     CDS             20758..21759
                     /gene="xylF"
                     /locus_tag="PXA33_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005576719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL5119646.1"
                     /translation="MKLKSKLLAVATATLMVLSNASVAKDLKIGMSIDDLRLERWQKD
                     RDIFVNKAESLGAKVFVQSANGDATAQISQIENMLNKDIDVLVIIPFNGEVLSNVIAE
                     AKKEGVKVLAYDRLINNADVDFYVSFDNEKVGELQAKSIIGQKPEGNYFLMGGSPVDN
                     NAKLFRKGQMKVLQPLIDSGKIKVVGDQWVDSWLAEKALQIMENALTANKNNIDAVVA
                     SNDATAGGAIQALSAQGLSSKVAISGQDADLAAIKRIIAGTQTMTVYKPITNLADKAA
                     EIAVALGKDEKVEMNAQLNNGMKEVPAYLLEPVVVTKENIDDTVIKDGFHTKDAVYK"
     gene            21826..23340
                     /gene="xylG"
                     /locus_tag="PXA33_000268"
     CDS             21826..23340
                     /gene="xylG"
                     /locus_tag="PXA33_000268"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011608742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HDL5119647.1"
                     /translation="MAKLLEMKNITKKFGEVVALNNISISLETGEILSLCGENGSGKS
                     TLMKVLCGIYPAGDYEGEIYFSGEKLIARNIKDTEEKGISIIHQELTLVKNMSILENM
                     FLGNEITKSGITDDNEMYLRCKTLLEQVQLDIDPNTKVGELGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEKETEILLSLIKDLQAHNIACVYISHKLNEVKAISDKICVIRDG
                     EHIGTRSAEGMSEDDIITMMVGREITSLYPHEPHNIGEEVLRVENLTAWHPTNTHIKR
                     VDNANFVLRQGEILGIAGLVGSGRTEMAQCIFGSYQGKYQGDIFLHKQPIKIKNCAQA
                     IENHIVMVPEDRKKHGIIPIMGVGKNITLSSLPQFCFGKKVINEPLEETIINQSIAKL
                     KVKTSSPELAIGRLSGGNQQKAILAKCLLLNPKILILDEPTRGIDVGAKYEIYKLINQ
                     LAKEGMAIIVISSELPEVLGISDRVLVMHQGKIKADLINQDLTQEKVMEAALKE"
     gene            23344..24471
                     /locus_tag="PXA33_000269"
     CDS             23344..24471
                     /locus_tag="PXA33_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005624872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL5119648.1"
                     /translation="MNKLKSINLQVYIMLIAIVVIMSFFSIATDGAYLSARNISNLLR
                     QTSITGILAIGMVFVIISAEIDLSVGSLMGLLGGFAAISNVWWGWPLSLTIIVTLILG
                     VMLGAWSGWWVAYQKVPSFIVTLAGMLAFRGVLVGLTNGTTVSPISNEMTTIGQGYLP
                     DMVGMILGTIGIFCFIVWGSYQRKARQKLNLTVPSMSKDTLKYGLVAIIVLGAIYLLN
                     DYRGVPFPVLVLVILAIAGTFLARKTAFGRHIYAIGGNIDAARLSGISVEKVKLAIFA
                     LNGLLVAIAGLILSSRLGAGAPSAGQNAELDAIAACVIGGASLAGGIGSIYGVVIGAF
                     IIALLDNGMSMLDVPTFWQYIVKGGILLLAVWVDTISKKKA"
     gene            complement(24487..25017)
                     /locus_tag="PXA33_000270"
     CDS             complement(24487..25017)
                     /locus_tag="PXA33_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL5119649.1"
                     /translation="MSENSLKTHYSAKELAELSLACLPKTKQGVLYQAKKQGWTTHKK
                     VGVGGEILEFALSNLPQEIQTEIRSRFMSAVVEAKPKKLPAVRAEVDVGNLTTKQREI
                     ADARMALVAYVLELEGSMSRIKAITYLCNLAKQGEMPPHLVELVAVANAKKTAKRTIV
                     PIFSPIMPDFPLDSRD"
     gene            complement(25033..25296)
                     /locus_tag="PXA33_000271"
     CDS             complement(25033..25296)
                     /locus_tag="PXA33_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL5119650.1"
                     /translation="MSTLEDIKKTAKDWHRADIIAALHKAGWSLRQLSLKHGYSQSST
                     LKNAVDRPWLKGERIIAEAIGISPEEIWPERYAQRNHKKFADR"
     gene            25480..25962
                     /locus_tag="PXA33_000272"
     CDS             25480..25962
                     /locus_tag="PXA33_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL5119651.1"
                     /translation="MKIECKKVIGFGNRIEQSRKNAKLSRSEVCDALGGIAISTLQAW
                     EADTREPPISKLMALSQILNVEPCYLLTGERHSQNLNVTREPQPVYATKPQTEEGEVV
                     VIKAKLQEAIETLEEVLEITQREMKPAGKAQMVWAFYELLTQEKSEKEKMVGLLKLVA
                     "
     gene            25974..26225
                     /locus_tag="PXA33_000273"
     CDS             25974..26225
                     /locus_tag="PXA33_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5119652.1"
                     /translation="MDKQAILKLFRDNSTAGNSQPSQEPIQVFNNSNVIIQNGDNATV
                     IAGCTVDNNTNLTCADKQRVHDLIHSLEQRNRASRDEGA"
     gene            complement(26515..28380)
                     /locus_tag="PXA33_000274"
     CDS             complement(26515..28380)
                     /locus_tag="PXA33_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HDL5119653.1"
                     /translation="MQSLLSFVPDFLKQELFWVMALLVFAIWQFIQNKLRMDVVALIV
                     MLFFSLTNILSVKEVLAGLSDPNVVLIALLFIVGEGLVRTGVANQVSDWLMRVSGASE
                     TKVLVLLMLCIAGLGSFMSSTGIVAIFIPVVLAICSGMGISPRRLMMPLSVAGLISGM
                     MTLIATPPNLVAHSELVKSGFEGFNFFSFTPIGLIILVMGIGYMLIARRWLDNGEQSN
                     THSQAQKFSMSQLIEEYQLKERAKMVLVEADSDCVGKSIGQLNLRIEQGLNIIAIQRT
                     KRFRKITVSASVNEIFQPKDILLLDTALDDETYQQRCEQLKLRPIELKGEYFSSQYRS
                     VGMAEISVMPEAECIGKTIRELKFRSRYHLSIVGLKRGDEIIQEELLDTPLKFGDILL
                     VMGVWQQIHRMDDDHRDFFLLRDPAESKNAPPALSQAPHALFSVLTMVLLMITGIVPN
                     VMAALIACLMLGKFRCVDMKSAYDSIHMPSIVLIVGMMPFSIALQKTGGVDLTVRLLL
                     DAMQGLDVHFVLIVLFVVTAIISAFISNTATAILVMPIAIAIANQLGYSPAPFVMTVA
                     VSASAAFMTPVSSPVNTMVLAPAGYKFIDFVKVGVPFTVLVMLVSVFVIPMLFPL"
     gene            complement(28520..29035)
                     /locus_tag="PXA33_000275"
     CDS             complement(28520..29035)
                     /locus_tag="PXA33_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MltR family transcriptional regulator"
                     /protein_id="HDL5119654.1"
                     /translation="MMDYIDKLSEETTLRGFLSIANQRFAKAVEQLIERVFRKTDFAL
                     KSVVDSLFEHQGPLADIEVRLKVLLGLGVISPKVFEDISLFLEVKQHLNEEEDEFAFS
                     HPAVVKFAKDLHHIDFLPINELLNMKASAANKDSMLFQMQQMRLEKMVRSSLILAVTE
                     IDEQLNVESPL"
     gene            complement(29047..30186)
                     /locus_tag="PXA33_000276"
     CDS             complement(29047..30186)
                     /locus_tag="PXA33_000276"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005557909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDL5119655.1"
                     /translation="MNALHFGAGNIGRGFIGKLLADARIFVTFADINQTQIDQINQNK
                     QYGVKIVGDDSRVEIVKNIAAINSKDENAVIEQVKNTDLITTAVGPNVLGFIAPLFAK
                     ALVARVESGNTQPLNIIACENMVRGTTFFKGKIFEHLTAEQQAEIEKVVGFVDSAVDR
                     IVPPAEPNPADPLEVTVEEFSEWIVDQTQFKGDIPNIKGMELTDNLMAFVERKLFTLN
                     TGHLICAYLGKQAGVKWIKEAIAIEEIKTQVKATMEESGAVLIKRYGFDPQAHSAYIE
                     KILKRFANPYLNDDVNRVGREPIRKLSENDRLIKPLRGTLEYGLPYQNLVKGVVMALQ
                     YRNEEDPQAVELAEFIANHGVAAAVEKYTGLTDQAVIAQVVELYQ"
BASE COUNT         8990 a         6219 c         6418 g         8595 t
ORIGIN      
        1 tttaagctac aaaaaacaac aaaaaacacg acaataagat cgagtaatga ttatattatg
       61 ttataatttt tgacctaatt tagaataatt atcgagtgca aattatgaat caatcttatt
      121 ttaacttact aggaaacatt acttggctat ggatgaactc ctccctccac aaagaatgga
      181 gctgtgaact actagcacgc aatgtgattc ctgcaattga aaatgaacaa tatatgctac
      241 ttatagataa cggtattccg atcgcttatt gtagttgggc agatttaaac cttgagactg
      301 aggtgaaata tattaaggat attaattcgt taacaccaga agaatggcag tctggtgaca
      361 gacgctggat tattgattgg gtagcaccat tcggacattc tcaattactt tataaaaaaa
      421 tgtgtcagaa ataccctgat atgatcgtca gatctatacg cttttatcca aagcagaaag
      481 aattaggcaa aattgcctac tttaaaggag gtaaattaga taaaaaaaca gcaaaaaaac
      541 gttttgatac atatcaagaa gagctggcaa cagcacttaa aaatgaattt aattttatta
      601 aaaaatagaa ggagacatcc cttatgggaa ctagacttac aaccctatca aatgggctaa
      661 aaaacacttt aacggcaacc aaaagtggct tacataaagc cggtcaatca ttaacccaag
      721 ccggcagttc tttaaaaact ggggcaaaaa aaattatcct ctatattccc caaaattacc
      781 aatatgatac tgaacaaggt aatggtttac aggatttagt caaagcggcc gaagagttgg
      841 ggattgaggt acaaagagaa gaacgcaata atattgcaac agctcaaacc agtttaggca
      901 cgattcaaac cgctattggc ttaactgagc gtggcattgt gttatccgct ccacaaattg
      961 ataaattgct acagaaaact aaagcaggcc aagcattagg ttctgccgaa agcattgtac
     1021 aaaatgcaaa taaagccaaa actgtattat ctggcattca atctatttta ggctcagtat
     1081 tggctggaat ggatttagat gaggccttac agaataacag caaccaacat gctcttgcta
     1141 aagctggctt ggagctaaca aattcattaa ttgaaaatat tgctaattca gtaaaaacac
     1201 ttgacgaatt tggtgagcaa attagtcaat ttggttcaaa actacaaaat atcaaaggct
     1261 tagggacttt aggagacaaa ctcaaaaata tcggtggact tgataaagct ggccttggtt
     1321 tagatgttat ctcagggcta ttatcgggcg caacagctgc acttgtactt gcagataaaa
     1381 atgcttcaac agctaaaaaa gtgggtgcgg gttttgaatt ggcaaaccaa gttgttggta
     1441 atattaccaa agccgtttct tcttacattt tagcccaacg tgttgcagca ggtttatctt
     1501 caactgggcc tgtggctgct ttaattgctt ctactgtttc tcttgcgatt agcccattag
     1561 catttgccgg tattgccgat aaatttaatc atgcaaaaag tttagagagt tatgccgaac
     1621 gctttaaaaa attaggctat gacggagata atttattagc agaatatcag cggggaacag
     1681 ggactattga tgcatcggtt actgcaatta ataccgcatt ggccgctatt gctggtggtg
     1741 tgtctgctgc tgcagccggc tcggttattg cttcaccgat tgccttatta gtatctggga
     1801 ttaccggtgt aatttctacg attctgcaat attctaaaca agcaatgttt gagcacgttg
     1861 caaataaaat tcataacaaa attgtagaat gggaaaaaaa taatcacggt aagaactact
     1921 ttgaaaatgg ttacgatgcc cgttatcttg cgaatttaca agataatatg aaattcttac
     1981 tgaacttaaa caaagagtta caggcagaac gtgtcatcgc tattactcag cagcaatggg
     2041 ataacaacat tggtgattta gctggtatta gccgtttagg tgaaaaagtc cttagtggta
     2101 aagcctatgt ggatgcgttt gaagaaggca aacacattaa agccgataaa ttagtacagt
     2161 tggattcggc aaacggtatt attgatgtga gtaattcggg taaagcgaaa actcagcata
     2221 tcttattcag aacgccatta ttgacgccgg gaacagagca tcgtgaacgc gtacaaacag
     2281 gtaaatatga atatattacc aagctcaata ttaaccgtgt agatagctgg aaaattacag
     2341 atggtgcagc aagttctacc tttgatttaa ctaacgttgt tcagcgtatt ggtattgaat
     2401 tagacaatgc tggaaatgta actaaaacca aagaaacaaa aattattgcc aaacttggtg
     2461 aaggtgatga caacgtattt gttggttctg gtacgacgga aattgatggc ggtgaaggtt
     2521 acgaccgagt tcactatagc cgtggaaact atggtgcttt aactattgat gcaaccaaag
     2581 agaccgagca aggtagttat accgtaaatc gtttcgtaga aaccggtaaa gcactacacg
     2641 aagtgacttc aacccatacc gcattagtgg gcaaccgtga agaaaaaata gaatatcgtc
     2701 atagcaataa ccagcaccat gccggttatt acaccaaaga taccttgaaa gctgttgaag
     2761 aaattatcgg tacatcacat aacgatatct ttaaaggtag taagttcaat gatgccttta
     2821 acggtggtga tggtgtcgat actattgacg gtaacgacgg caatgaccgc ttatttggtg
     2881 gtaaaggcga tgatattctc gatggtggaa atggtgatga ttttatcgat ggcggtaaag
     2941 gcaacgacct attacacggt ggcaagggcg atgatatttt cgttcaccgt aaaggcgatg
     3001 gtaatgatat tattaccgat tctgacggca atgataaatt atcattctct gattcgaact
     3061 taaaagattt aacatttgaa aaagttaaac ataatcttgt catcacgaat agcaaaaaag
     3121 agaaagtgac cattcaaaac tggttccgag aggctgattt tgctaaagaa gtgcctaatt
     3181 ataaagcaac taaagatgag aaaatcgaag aaatcatcgg tcaaaatggc gagcggatca
     3241 cctcaaagca agttgatgat cttatcgcaa aaggtaacgg caaaattacc caagatgagc
     3301 tatcaaaagt tgttgataac tatgaattgc tcaaacatag caaaaatgtg acaaacagct
     3361 tagataagtt aatctcatct gtaagtgcat ttacctcgtc taatgattcg agaaatgtat
     3421 tagtggctcc aacttcaatg ttggatcaaa gtttatcttc tcttcaattt gctagagcag
     3481 cttaattttt aatgattggc aactctatat tgtttcacac attatagagt tgccgtttta
     3541 ttttataaaa ggagacaata tggaagctaa ccatcaaagg aatgatcttg gtttagttgc
     3601 cctcactatg ttggcacaat accataatat ttcgcttaat ccggaagaaa taaaacataa
     3661 atttgatctt gacggaaaag ggctttcttt aactgcttgg cttttagctg caaaatcgtt
     3721 agcgttgaaa gcgaaacaca ttaaaaaaga gatttcccgc ttacacttgg tgaatttacc
     3781 ggcattagtt tggcaagata acggtaaaca ttttttattg gtaaaagtgg ataccgataa
     3841 taaccgctat ttaacttaca atttggaaca agatgctcca caaattctgt cacaagacga
     3901 atttgaagcc tgctatcaag ggcagttaat tttggtcacg tccagagctt ccgtagtagg
     3961 tcaattagca aagttcgatt tcacctggtt tattccggcg gtgatcaaat accgaaaaat
     4021 ctttctagaa accttgattg tttcgatctt tttgcaaatt tttgccctaa ttacaccgct
     4081 attcttccaa gttgttatgg ataaagtact ggtgcatcga ggtttttcaa ccttgaatat
     4141 cattacggtt gccttagcta ttgtgatcat ctttgaaatt gtactaagtg gtttgagaac
     4201 ctatgttttt tctcatagca ctagccgtat tgatgttgaa ttaggcgcta aattatttcg
     4261 acatttatta tcactaccca tttcttattt tgaaaacaga cgagttggag atacagtcgc
     4321 tagggttaga gaattagatc aaattcgtaa tttccttacc ggacaagcat taacctcggt
     4381 gttagatctc ttattctctt ttatcttttt tgccgtaatg tggtattaca gcccaaaatt
     4441 aaccttggta attcttggtt cattgccctg ctatatttta tggtcaattt ttattagtcc
     4501 gattttaaga cggcgtttag atgagaaatt tgcccgaagt gctgataacc aagcattctt
     4561 agttgagtcg gtaacagcca tcaatatgat taaagcgatg gcggttgctc cacaaatgac
     4621 ggatacatgg gataaacagc tggcaagcta tgtttcatca agtttccgtg tcaccgtatt
     4681 agcaaccatt gggcaacaag gtgtacaact tattcaaaaa accgttatgg tgattaacct
     4741 ttggttaggg gcacacttag ttatttcagg cgatctgagt attgggcaat taattgcctt
     4801 taatatgcta tcagggcaag tgattgcacc ggtgattcgg ctggctcagc tctggcaaga
     4861 tttccaacaa gttgggattt ccgtcactcg cttaggtgat gttttaaact ctccaaccga
     4921 acaatatcaa ggcaaattat cactaccaga aataaaaggc gatatctcat ttaaaaatat
     4981 ccgctttaga tataaaccag atgcaccaac tattttaaat aatgtgaatt tagaaattag
     5041 gcaaggagaa gtgattggga ttgttggacg ttccggttca ggcaaaagta ctctgactaa
     5101 attactgcaa cgtttttata ttcctgaaaa tgggcaggtt ttgattgatg gacatgatct
     5161 agccttagct gatccaaact ggctacgccg tcaaataggt gtagtgctgc aagataatgt
     5221 gttattaaac cgcagtatcc gagaaaatat tgcgctatca gatccaggaa tgccaatgga
     5281 gcgagtaatt tatgcagcaa aattagcagg ggctcacgat tttatttcag aattgcgtga
     5341 aggttataac accattgtgg gtgaacaagg agcggggctt tcaggcgggc aacgccaacg
     5401 gattgcgatt gctcgagctt tggtaaacaa cccgaaaatc ctgatttttg atgaggcaac
     5461 cagtgccctc gattacgaat ctgagcatat tattatgcaa aatatgcaaa aaatatgcca
     5521 aggcagaacc gtgattttga ttgcacatcg tttatcgacc gtcaaaaatg cggatcgaat
     5581 tattgtgatg gaaaaggggg aaattgttga gcaaggcaag caccacgaat tactgcaaaa
     5641 cagtaacgga ctttattcct acttacacca attacaactt aattaagaag gaaaacaatg
     5701 aaaatatggc ttagtggtat ttatgaattt ttcctacgct ataaaaacat ttgggcagaa
     5761 gtatggaaaa ttcgtaaaga attagaccac ccaaacagaa aaaaagacga aagtgaattt
     5821 ttaccggcac atttagaact gattgaaacc ccggtttcta aaaaaccacg tctaattgct
     5881 tatttgatta tgctattttt agttgtggca attgtgcttg ccagtgtaag caaagttgaa
     5941 attgtggcga ctgctcccgg taaattaact tttagtggca gaagtaaaga aattaaaccg
     6001 attgaaaacg ccattgtaca agaaattttc gttaaagatg ggcagtttgt ggaaaaaggg
     6061 caattattag tcagcttaac tgcattgggt tctgatgcag atatcaaaaa gaccatggct
     6121 tcactttctt tagctaaact ggagaactat cgctaccaaa ctttgcttac tgccattgaa
     6181 aaagagtcct tgccggtgat tgatttatct agaaccgaat ttaaagattc atcggaagaa
     6241 gatcgactac gtattaaaca cttaattgag gagcaataca ccacttggca aaaacaaaaa
     6301 acacagaaaa ctttagcgta taagcgtaaa gaggctgaaa aacaaacaat atttgcctat
     6361 gtccgtaaat atgaaggtgc aacacgtatt gaacaagaaa aattaaaaga ctttaaggca
     6421 ctttataaac agaagtcttt atctaagcac gaacttcttg cgcaagaaaa taaattaatt
     6481 gaggctcaga atgagctagc tgtttatcgc tcaaaattaa atgaattaga aaatgatcta
     6541 ctcaatgtaa aagaagaact tgaattgatc acgcaattct ttaaaagcga tgtgttggaa
     6601 aaattaaagc aacatattga aaatgaacgc caacttcggc tcgagttaga aaaaaataat
     6661 caacgcagac aggcctcgat gatcagagca ccggtttccg gtacggttca gcaactgaaa
     6721 attcacacta taggtggtgt tgttacgact gctgaaacct tgatgatcat tgtgccggaa
     6781 gacgatgtgt tagaggccac cgctctggtt ccaaacaaag atatcggctt tgttgcagca
     6841 gggcaggagg tgattattaa agtggaaact ttcccttata cacgctatgg ttatctaact
     6901 ggtcgaatta aacatattag cccggatgcg attgaacaac ctaatgtagg cttagttttt
     6961 aatgcaacta tagctataga taggaagaat ctaacatcgc ctgatgggcg aaaaattgat
     7021 ttgagttcag gtatgacaat aactgctgaa atcaaaaccg gtgaacggag tgtaatgagt
     7081 tatttactca gcccattaga agaatctgtc acagaaagtt taagggaacg ctaatcgaac
     7141 caaaacaaag ccataaaagc cattttgagc ttttatggct ttatttttta gtccacaagc
     7201 ggtcaaaaaa gcccaatttt ttacactttt ataacaaatt gttctaacta aaaattacta
     7261 attcttttct tttataagac gaatcctcta ttttcatttc attaacattg aactagaaaa
     7321 ggggatttat gagcctaagc attacgaatc tttctcttgg ctaccgcaaa aatcagcaaa
     7381 ggcttatttg aaaagcacgg tgtgcaggtg gaaaaaccgg tgatgtttcg cagctgggct
     7441 cagttggtgg aagccttttt aagtggcaat gtgaacgtgg tgcatctgct ttcgcctatg
     7501 agtttgtggg cgaaatatgg agcaaatgct ccggtgaaag cggtaatgtg gaatcacttg
     7561 gcaggttcgg ctttaacggt tcgccctgaa atcaacagta ttgccgaact ctccggcaaa
     7621 acggtagcaa ttccgttttg gtattcaatt cataatatcg ttttgcaacg attattacga
     7681 gaaaatggct tgaaaatcac cgaaaaagag ccgaaaagcg gtgaagtgcg attaagtgta
     7741 atggctccgt cagatatggt ggcagcgttg gcaagtgagg caatttccgg ctttattgtg
     7801 gcagagccgt tcaatgcgtt agcggaagcc aaaggtgtgg ggaaggtgct gcgttttagt
     7861 ggcgatgtgt ggaaagatca cgcctgctgt ttaaccctaa tgcacgaata cgatattcaa
     7921 caccgcccgg aatgggtgca aaaagtggtg aatgtcttaa ccgaagccca agtgtccacg
     7981 cttgccaacc gtgcagaaac cgctcaatta ttggctcggg gcagcggcta cacgccgcac
     8041 gatcaaaaag tgctggaaaa ggtactggct ccaacccaag ctcagtggga taaatatatc
     8101 gaaacagggg caattcaaaa ccctcagtgg caccaacagc gaatcggttt ccaaccgtat
     8161 ccgttcgatt cctatatgga agagctggtc aaaatgctca aacagaccca cattgccggc
     8221 aataatgcgt ttttacaaca gcttgacccg gtagaggcag cgaaagcgat taacgagccg
     8281 acctttgtgc gaaaagcgat tgagcaaggc ggttgggttg aaacctttaa tttacaaacc
     8341 ggttggacaa gaaccgagca aattagtgta taagcgatag gaacgaacga tgaaacagaa
     8401 atatatttac ggcttagccg gtttattact gctactttta ggttggcagc ttggcacagc
     8461 cttgttaagc caacggttgc cgatggcgtc aatgttagct ccggcaaaaa cctgggaaag
     8521 ttttacttgg ctgatttcct ccggcgaaat ttttccgcac ttatgggcga gtttgcaacg
     8581 cattgctgtc gggctactcg ctgcgttagt gattggtgta ccgctgggct tattgctcgg
     8641 cttctccaag caccttgaaa tgctttgcac gccgtcattc caactactac gtatgatttc
     8701 gccactttcg tggatgccga ttatcgtgat gttacttggt gtaggcgatt tgccgattta
     8761 ttttttactg gcgtttgcca ccgtttggtc gttgattatt tccaccacct caggggtaaa
     8821 aagcattgat ccacaatggt tacaactggg tagagtaatg tcggctaccc gcaaagaact
     8881 gctgtgcaaa attgttgtac cggctattac cggacacgtt ctcaccggtt tacgtgtggc
     8941 ggttgggatt gcgtgggtgg tgctggtacc gtgcgaaatg ctaggcgtga atcaaggact
     9001 cggctatttt attttagaca cccgagaccg attagcctat tccgaactga tggcggtaat
     9061 tataattatc ggcttaatcg gctgggcgtt ggatagctta atccgcttgg ctttagcgaa
     9121 aggcggcaga taaattgaca agcggtcgaa aaattgcatt tttttgcaaa aaatcgaccg
     9181 cttgtttatt cccaaaaagc atattacctt aatttatttt atttctaggg cataaaaaag
     9241 cgatttaaga tagcgacaaa gtaaactaat tatacgaggt aaacacaatg tcggttcttt
     9301 catcttcttt tattcaatgg cttcagcaga acgccgaaaa tttagatcaa tctaacgaac
     9361 gagcggattt actcattcca caaattgccg cactagggct atataaaatc ggcgtgccgg
     9421 aaacgcttgg cggcagcggt ggcactttgg ttgatgcgat caaagccgtt accgaactgg
     9481 gtaatcactc gctggctgcg gcatttgttt cgtggggaca tcgtaccttt atcgagcata
     9541 ttatcgcctc aaaaaatcct attccccgcc aaagctattt ggcggatctg ctaaatggca
     9601 atttaaccgc aggaacaggt ttatccaatg cgatgaaatt tctctccggc attgaagagt
     9661 tgaatgtgaa tattcgtgaa gaaaacggta aacgctattt aaatggccgc ctgccttggg
     9721 taaccaattt acgttcatac cgctttgttg ctagtttcgt tgccggtttt caagacgaac
     9781 gcaaaccgct agtgattgca attccctcca ccgccaaagg cttaacccgc accaaagaat
     9841 tggaatttat cgcattgcaa ggcacgaata ctacctctct cgtatttgag aatgtggaat
     9901 tacaagatga atggattttg gctgatgacg cgcctgagtt tttagcagcc actcgccctg
     9961 cgttcttagg tttacaattt ggtttgccct ttggcttggt tgaacgtagt ttggcggaaa
    10021 tcgaaaaatc gctccctttc cgtgcaattc tcgcctctga gtggcaagct caagttgatg
    10081 ccctcaacga tattaaaact cgtctgttta atggcttaaa tgaagaaggt tattttgtgc
    10141 gtaatccgaa agagcttttc caagcgagaa ttgacattgt caatgtcgtc gcccaaacta
    10201 ttctgctaga actgcaagcg agtggcggac gtggctatct gaaaaatgat acttcaggct
    10261 tcagccgccg ttggaaagaa gcggcatttc tgcccgttgt tacgccaagt gcggtacagc
    10321 tgagaatggt gctggcgaat agttaagcgg ataagagaca aattgttgcc tcttttattt
    10381 tgcaaaaaat agtctaaata taaccgcttg caataaaaat aaaaaaggat gcatttttta
    10441 tgcttcctca attttacggc tatctattat gcaaaaggct gcaattcagg gttaattgga
    10501 atttcggcaa tgttgttaac gtagttacat aatgttgcca agctcacgcc taaaatcaca
    10561 tcaatcgcat tttgttggtt gtaacctgcg gcaaaaaatt cgtttaattg ggcttcggtt
    10621 aatttggctt tttgtaacat tactgcaagg gtgaagcgag ccagagtgtc taatttgtta
    10681 tcgtctatgc gagccgtggc acgtaaagca tttaccactt cttcaggcat ttttaatgct
    10741 tttaaggcga ttttagtatg acctgccacg cagaaaccac agccgttgac cactgctgcg
    10801 gtgatttgta ccacttcacg ttcttcagcg gttaagctgt tacgactatt aataccaccc
    10861 acagtgcggt aagtttccag tgctgttggt gcgttggcta atacgccgat cagatttggg
    10921 ataaaaccgt ttgcttgttg cactgctttt agggcttctt gtgccgtttc aggagcggat
    10981 tcaatagtgt gaatttgaaa ttgagacata aatgttcctt atcaggttgt ttattggtgt
    11041 ggctcatcat aaagaaaagg ctaatttaga acaaatcaaa atcactattt aatataacta
    11101 aaagatctta aagggagtat agttgcctat actccctttg tttatgcaca ggttgcgttt
    11161 ttactcttct gcgtcttctt ttgcccattc taatgagcgg cgaacggctt ttttccaacc
    11221 tttatagcgt ttcgcccgtt tttcttggtc gccgtccggt gtgaaggtgc gttcaatttc
    11281 cgctttgtct ttcagttctt ccaaatcttt ccagaagccg acagcaatac ccgcaagata
    11341 agctgcccct aaagcagtga cttctttcac tttcggacgc tcaactttgg tgtctaaaat
    11401 atcggcttgg aactgcatta agaagttgtt tgcggttgca ccaccgtcca cacgtaacgc
    11461 ttgtagtttc gctttggagt cagactgcat tgcttctaac acatctcggg tttggtaagc
    11521 gatagactct agggtcgcac gcacaatgtg gttacggttt gccccacggg ttaaaccgaa
    11581 aattgagcct cgtgcatacg gatcccaata tggagcacct aagccggtaa aggcaggtac
    11641 aacataaaca ccgttagagt catcttcttt agtggcaaaa tattcgctgt caaagctatc
    11701 gtgaacaatt ttcagctcat cacgcagcca ttgaatagac gcaccggcaa taaatactga
    11761 accttccaac gcatattccg gctcgccttt tgcgttacac gcaatggtcg ttaataaacc
    11821 gttttcagat tgaatcgcag tgttaccggt gtgcatcaac ataaaacagc ctgtaccata
    11881 ggtattcttc gcttgtcctt ctttcacgca taaatgaccg tagagagcgg cttgttggtc
    11941 acctgcaata cccgcgaccg gaatacgcac accgccttga ccgccgatat tagtttgccc
    12001 gtaaacttct gatgaatttt tcacttcagg taacattgag cgtggaatat tgagcaattc
    12061 cagcatttta tcgtcccatt gtttggtgtg aatgttaaac aacatagtac gagaagcgtt
    12121 ggtgtaatcg gtaacgtgta cccgcccttg ggttaatttc cacaccagcc aagtatcgac
    12181 cgtaccgaac agcaattcgc cacgttctgc tttttctctt gccccttcta cgttatctaa
    12241 aatccacttc actttagtgc cggagaagta cggatcgact accaagcctg tcgttttacg
    12301 aatatattcc tcgtgtccgt ctgcttttaa tttatcggta atatccgatg tgcgacgaca
    12361 ttgccacaca atcgcattat aaacaggttt accggtctct ttttcccaca caatcgtggt
    12421 ttcacgttgg tttgtgatcc cgattgcggc aatgctatcc ggcttaatac cggcttttgc
    12481 caccacttca tttaaggtgg aactttggct tgaccaaatt tccatcgggt tatgttctac
    12541 ccagcctgct tgcggataaa tctgggtaaa ttcacgctga gccacttcaa ccacattggc
    12601 atttttatct aataaaaccg cacgagagct ggttgtacct tggtcgagag cgatgatata
    12661 ttgtttttcc atagattact cctgagtatt tgctggaaga ttgttatgaa tgaggttttt
    12721 ataaccccaa gcaccgaata atgcaccgca gattggggcg acaatcggaa tgatgaaata
    12781 cggaatttct tttccgcctg ttaaagcaat ttcaccccaa ccggcaagat aagcaaagag
    12841 tttcggacca aaatcacgag ccgggttcat cgcaaagccg gttaacgggc cggttgctcc
    12901 accaattacc gcaatcaata aaccgatgag taatgcggct aaggctttat tcggcaagcc
    12961 gtttttatca tcgccaatcg ctaaaatagt ggacatcaac accacagtaa tcacaaattc
    13021 aacgatgaag gcggtaacca atgtaatatt agggtttgga taggtggaga acacaccggc
    13081 aaagccaaca gtttcgcccc gagtaatgcc ttgagcggct tcggcggcag taaagagatc
    13141 gctatacata aaataaatca gtgcgacaga gcaaaacgct ccggcaactt gggcgatgac
    13201 atagggcaaa attttatgct tttcaaagcc gtagaatgcg gcaagtgcta tagtgaccgc
    13261 agggttaagg tgagcaccgg aaataccggc agaaatgtag attgccatgg aaacaccaag
    13321 cccccaaacg atggaaattt cccataaacc gaaattcgca ccggctacct gagcagcagc
    13381 aacacaacca gcaccaaaga aaagaataaa acctgtaccg ataaattcgc caatacaggc
    13441 attacgtaag gatttgctca taaagtttct ccttaagatg aaaatgattg gaaagtgatg
    13501 aaaaaatgtt tgaatatgaa catatttcgg agtgatattg ccaaagcggc attttttaag
    13561 caaataggaa gtttcaaatt tgtgaaatcc atcacattat ttttctaaat cactcatttt
    13621 taggtatgat gtgagaaaac gttagatgag gaaaaatgat gtcttcaata cttatgattt
    13681 caatcggggc ggtacttgga gctatgtgcc gctggcaatt agccgtttgg ctaaacccga
    13741 tactcagcca attcgccttt ggtactttag cagcaaattg gcttggttgt ttgttaattg
    13801 gtattgcaat ggggtttaat ttaggcgaga gccagcggtt gctgttcatc agcggttttt
    13861 taggcagttt taccaccttt tccagtttct ctttagaact aacggaaaaa ctattagcag
    13921 aaaaatgggg gcagtttgtg acagtgctga gtttacattt aatcggtggg cttttgctta
    13981 ctttgatagg tgtaatgctt gcaagagccg ttacaagcgg tcgtttttgc tgaaaatttt
    14041 gcaaataaaa aacctcccaa acgggaggtt ttgcgagcga ttaaagtagg atattggaaa
    14101 gtaataaacc aaatccgata ctgaaaatca tactaagtaa gcccggtaac ataaagctat
    14161 ggttgaaaat atatttaccg atacgggttg tgccggtggt gtcgaagtca atcgaggcaa
    14221 taatcgggcc gtagttcgga atgaagaagt agccgtttac cgccacaaat accccgatta
    14281 gcaccggtgc aggaatgccg atagcaatcg ctacagggaa taaggttgca accgttgccc
    14341 cttggctgtt taccaatacg gaaagtacga ataatgcgaa agcaaatgcc caaggtgcgg
    14401 tttcaactaa gcctgaaacc attgatttca cttcgtccat atgaccttgc attaaggtat
    14461 cgcctaacca agcaataccg aaaatcgcaa tcactgcacg cataccggcg tggaagacag
    14521 aacctttggt gatttcatta ccgtccggtt tgcagaagaa gataatcaac gcaccaatac
    14581 ttaacatcac aatttcaatc gtgtgagcca tccccatcgg cttaccatca aacacagggc
    14641 gaagagctgg aattgcgccc attaatacca ctaataatgc accgaatagg aataagccta
    14701 ctgataattt cgcagtcggt tttaacggta attcggttgg attggtagta atgtgatttt
    14761 ctttcacata ttcagggtct tgcaataagc gttgataatt tgggtcgtct tttaattctt
    14821 tgcccatttt atttacaaac acacaagcta aacctaagcc caataaggtc gcaggaatag
    14881 tcacacttaa cacgttaccc aatgtaacac cttgaggttc aaggaaggca accacagcaa
    14941 ctactgccgc agcaatcggg ctggcgacaa tcgcaaattg tgaggcgatt actgccattg
    15001 aaagtggacg ctcagggcgg ataccgttgt ggcggcttac ctctgcaatt acaggtaata
    15061 cagaataagc aacgtgacct gtaccggcaa gcacggtgaa tgtccaagtt acaagcggtg
    15121 cgataaaggt aatgtatttc gggtttttac gcaaaatgcg agtggcaatt ttgatcatat
    15181 agtccaaacc accggctgct tgcatggctg ctgcagccga cacaaccgcc ataatcataa
    15241 acattacatc aatcggtaaa ccggcaggtt ttagcccaaa cccaaatgaa agaatggcta
    15301 agcccaagcc accgaacacc cctaatccga taccgcctac acgggcgcca accaaaatgc
    15361 atagtaacac tatggcaaat tggataataa acatcgcaga cataattttt tgctcctaaa
    15421 taagtaaatg aaactctgtg tttgaaaaaa tctgcatttt tcatcaaatt aatcggcacg
    15481 tactatatca ataaataaaa ctaatctcta taatctaaat caataaaatt tctaattaat
    15541 taaggaataa tatgcaattt tcaaaaatgc acggcttagg caatgatttt atggtggttg
    15601 atggtgtaac ccaaaacgtt taccttaccg aagagatgat ccgtacgctt tctgatcgcc
    15661 atcgtggtat cggtttcgac caacttttat tggttgaacc gccttacgac cctgaattag
    15721 attttcacta tcgcattttt aatgcagacg gcagtgaagt gtcgcaatgt ggcaatgggg
    15781 cgagatgttt tgcccgtttt gttacgctca aaggcttaac caacaaaaaa gacattcacg
    15841 ttagcaccgc aaaaggcaaa atggtgctaa gtttgcaaga agacggcaaa gtgcgtgtga
    15901 atatgggcga accgatttgg gaaccttcgc aagtgccttt tacagcaaat aaatttgaga
    15961 aaaactatat tttacgcacc gagctacaaa ccgtgttatg tggcgtggtt tcaatgggga
    16021 atccgcactg cgtactgcaa gttgatgatg tgaaaaccgc cccggtaaat gaactcgggc
    16081 cgttactaga aagccacgag cgttttcctg aacgggcgaa tatcagcttt atgcaagtag
    16141 tgaatcgcaa tcacgttaaa ttacgggtgt atgagcgtgg agcaggcgaa acccaagcct
    16201 gtggtagcgg tgcgtgcggc acagtggcgg tcggcattat gcaaggcttg ttagataaca
    16261 aagtgcaggt ggatttaccc ggtggaacgc tgattattga atggcaagga gtagggcatc
    16321 ctctgtatat gaccggcgat gccacccaca tttatgatgg atttattaaa ctgtaagttt
    16381 tcccataggg aggcaagcgg tttattttta ccgttttttt gcaaaatagg gttggtttat
    16441 acaaaagtac cagcgtggac gctggcacta tcagactgat gtataacttt tgtttaaatt
    16501 caacttatag atggtgcaag cgtcctcgct tgtaccaaaa caggtttcac tcgagtaact
    16561 tcttcaccaa ctccaacacc acaggtaatt cggtaacacc ttctctatcc aaaaaatgcc
    16621 cgccttgatt taaccgaata taatccgcgt gcaaatgttg ggctaacgca tcactgtatt
    16681 ggtgattcac gaccttatca tctaacgcac ttaccacata gcttggaaag ctcgatacgc
    16741 tcggcaaaat cgcataatag ttggtaaagg cagacagttc gggtaagttt tctaccggtt
    16801 gatagaaccc cgacacaaac accgcccctt taatcttggc gtaatttttg gctaaaaaat
    16861 tcaaaatggc aatgcaacct aaactatgcc cgacaaaaac cgtttgatcg gtcagctcca
    16921 cattttgtgc aagatgattt aaccaaccat ctaatgacgg ctttgccgaa tccggcatat
    16981 tcaaccgctc acaactgaca ccacacttcg ctaattcttg ctccagccaa ggaaaccaat
    17041 gcttatcggc attcgccgta taaccgtgta cgatataaac ctgtgccatt tcacacctcc
    17101 tgattcacca aagattgtac catagcttaa tcaggaaaaa acaagcggtg atttttttaa
    17161 atttttttgc aactcaggta aaatagcacg catttacgca aagaggtcat caatgaacgt
    17221 ttggatttta cttgcgattt ccatctgcct cgaaatcgct gccaccaatt tactcaaatt
    17281 aagcaacggc ttcaccaaag ctataccgac tattggctcg ctcgccttat acggtttatc
    17341 cttttacttt ctctctatta ttttccgcac cctaccggtc ggaatcgtgt atgctatttg
    17401 gtccggcgta gggattgtcc ttaccgccct tgttgcttat tttgctttcg gacagaaaat
    17461 tgacttggca ggcttaatcg gcatcagcct cattattgcc ggtgtattgg tgattaattt
    17521 gttttctaaa gtgtgataca agcggttgat tttacaaaaa aatctgcaaa atcaaccgct
    17581 tgtaatagtt ggcaagaatt ataacccctt taaccgccgg taaagctcag taaatttttg
    17641 gcgtttttgt tgatattctg catagcgggc aggggttggt tggtaaacct gttccagggg
    17701 taacggctga cagaacgtag caatatcctg atttgggttt agcgcaattt gagcgagttt
    17761 tgccgcccct aatgccggac cgacatcacc actggtgcgg tattcaaagg ttttaccgct
    17821 aatatcggca agtagttgtc gccagtacac actttttgct ccaccgccaa ttaatgcaat
    17881 attgtcagca actacaccag tttcgtggag tacctcaatc ccttcagcca aagcgaaact
    17941 taccccttca atcacggctt ttgccatcgt tatttggttg tcattatggc ttaatcccca
    18001 aaatacgcct ttcgcataag ggtcattatg cggtgtacgt tcacccgata aataaggtaa
    18061 gaaaatagca tcagatgccg tttggcttgc ttcaacctgt tggaataaag tcgatacatc
    18121 ttttagtccc aatgatttta ctgcccaatc taccgcagaa gcggcactta atattactga
    18181 catcagatgc caacgatcag gtaaagcgtg gcaaaaacta tgcactgctt tttgcggatt
    18241 agcctgaaat ttatcgctca ctacaaaata aacccctgaa gtccccaatg agagcatagc
    18301 ttgcccggtt tggtataagc caatgccgat tgcaccggca gcattatcgc caccgcctgc
    18361 cacaatcggc acagatttca ttttccattg ctcggcaagg gttggacgta aataacctgt
    18421 gatttgattg ccttcaaaca gtttcggcat attttcaatg tctaatccgc aagcatttag
    18481 tagcgatttg ttccaattcc gtttacccac atcaagccac attgtgccgg aggcatcaga
    18541 catatcagag gcatattcgc cgctcatcaa caagcggaga taatctttcg gtagcaagac
    18601 tttgctgact ttttcggcaa tatcgggctg gtgtttatca acccaacgca atttcggagc
    18661 ggtaaagccc ggcatcatca ggttgcctgt aatttcacgg ctatttggca caagctgttc
    18721 taactcttcg cattccataa aactacgtcc gtcattccat aaaatcgcag gggaaagtac
    18781 attatctgcg ttatctagta aagtcgctcc gtgcatttgc ccggttaaac caatcgcttt
    18841 tactgcttgt aaatcttgag tagcggaaag ttctaacatc gcttcattgg tggcattcca
    18901 ccaatcttgc ggattttgtt cagaccacaa tgggtgtgga cgaaaaatag gaagtgattt
    18961 ttgtgtgatg gcaataatgt gttgattttc atcgagtaac acgactttca cacccgatgt
    19021 gccgagatcg attccaatgt acataaatat tcctttgtta ttgaatacaa gcggtcattt
    19081 tattcggaac atttgcaatt ttaagttttg gagatctatc acccctcttt agcaaagagg
    19141 gggatcagat cgagcttaac tttgcaaaaa attgataaaa aacaaccgct tgtagattaa
    19201 cggtaaatat agctatttac caaattttcc aaatattctt gctgaccgga aacaggtttt
    19261 ggatctaacc cttgggtttc cactattttt gccaaatctt caaggcttgc tttaccgttt
    19321 agaatttgta agcccaaatc ttggttccag ccagcgtagc ggttatctac tactttctgt
    19381 aatgcttgat cttccagcat ttttgccgca cgttttagcg aaagggctaa cacatcaatt
    19441 gccccgatat gtgcgtggaa taaatcatac ggatcggtac tttggcggcg gattttcgca
    19501 tcaaagttga aaccgcccgt tgtgaaacca cctgctttta agatttcata catcacaagg
    19561 gtgttttctt ccacgctgtt tgggaattgg tcggtatccc aacctaattg cggatcacct
    19621 cggttggcat caatcgagcc aaaaatatct aatgcggtgg cagtcgcaat ttcgtgttgg
    19681 aaagtatgcc ctgcaagagt ggcgtggttt gcttcaatgt tgactttgat ctctttttct
    19741 agaccaaact gttttaagaa accgtaaacg gtggcaacat cgtagtcata ttgatgtttg
    19801 gttggttctt gtggttttgg ctcaattaat aaagtgccgt taaaaccgat tttgtgttta
    19861 tgttccacca ctaactgcat aaagcgaccg atttgttcac gctcacgttt taagtcagtg
    19921 ttgagtaggg tttcataacc ttcacgtccg ccccataata cataattttc gccgcctaag
    19981 cgtttggttg cgttcattgc ggtaaagact tgtgctgccg cccaagcaaa gacttccgga
    20041 ttcggattgg ttgaagcacc cgacatatag cgaggattgg taaagcagtt cgccgtaccc
    20101 cataataatt tcacgcctgt ttcagcttgt ttttgctcta aaatatccac aatagtgttg
    20161 aaattgtgct gatattcttt gaaattgttg ccttccggtg caacatccac atcgtggaag
    20221 cagtagtaag gaatgcctaa tttttggaaa aattcaaagg caatttccgc tttttgcttc
    20281 gcaccttgca gtaaatcccc cgttttttgc cagcttctgt caagcgaacc cacgccaaac
    20341 atatcgttac ccgtccagca gaaggtgtgc caatagcaaa ccgctaaccg cagatgttct
    20401 gccatcgttt tgcctaaaac cacttcatcc gggttgtaat gtttgaatgc aaacgggttg
    20461 gtggaattag ccccttcata ttgtactttt gagattttgt cgaaatagtt agacataata
    20521 atgctcctat gaattggaaa aataatcttt cggtaacatc atcggaaaat tatgcaaaac
    20581 cttcaattac gatatttcgt ttttggattt cgattattgg ttaatgtggc gtagatcaca
    20641 aaagtgaaaa atcataatga tattaataaa tatgataatt gaacttcatt tgaaataagc
    20701 ccagactgct tacgggcgaa aaagatgctc acattttcat aaccaatgag gtgaactatg
    20761 aaacttaagt ctaaattatt agccgttgct acggcgactt taatggtact cagcaatgct
    20821 tctgttgcta aagatctaaa aatcggtatg tcaattgatg atttacgctt agagcgttgg
    20881 caaaaagacc gagatatttt tgtgaataaa gcggaaagct taggagcaaa agtcttcgtt
    20941 caatcggcaa atggcgatgc aaccgcacaa atctcacaaa ttgagaatat gttgaataaa
    21001 gatattgatg tattggtgat tattccgttt aacggcgaag tgttatccaa tgtgattgcc
    21061 gaggcgaaga aagaaggtgt taaggtactt gcttacgacc gcttaatcaa taatgccgat
    21121 gtggattttt atgtttcatt tgataatgaa aaagtaggtg aattgcaggc taaaagcatt
    21181 attgggcaaa agccggaggg gaattatttc ctaatgggtg gttcacctgt agataataac
    21241 gcgaagctgt tccgtaaagg tcaaatgaag gtgctacaac cgttaattga cagtggaaaa
    21301 attaaagtgg taggcgatca atgggtagat tcttggcttg ctgaaaaagc attacagatt
    21361 atggaaaatg cactaactgc taataaaaat aatattgatg cagttgtagc ttcaaacgat
    21421 gcaaccgcag gcggtgcaat tcaagcgtta agtgcccaag ggttatccag caaggttgca
    21481 atttccggac aagatgcaga tttagccgca attaaacgta ttattgccgg tacgcaaacg
    21541 atgacagtgt ataaaccaat taccaattta gccgacaaag ctgccgaaat tgctgttgca
    21601 ttaggtaaag atgagaaagt agaaatgaat gctcaattaa ataacggtat gaaagaagta
    21661 ccggcatatt tattagagcc ggttgttgta actaaagaaa atattgatga cacagtaatt
    21721 aaagacggct tccataccaa agatgcagta tataaataaa tcactttaat ttttctagat
    21781 ggcgaggctt cctcgccatt tttgaaaata aggggctaac taagtatggc gaaattatta
    21841 gaaatgaaga acatcactaa aaaattcggt gaggttgtgg cattaaataa tatttcaatt
    21901 tcccttgaaa ccggcgaaat actttcttta tgtggtgaaa atggttcagg caaatcaact
    21961 ctgatgaaag tattatgtgg aatttatcct gccggtgatt atgaagggga aatctatttt
    22021 tccggcgaaa aattgattgc cagaaatatc aaagatactg aagaaaaagg catttcgatt
    22081 attcaccaag aattgacctt agtaaaaaat atgtcgattt tggagaatat gtttttaggg
    22141 aatgaaatta ccaaatcagg gattacagat gacaatgaaa tgtatctgcg ttgtaaaacc
    22201 ttacttgaac aagtgcagtt ggatattgac ccaaacacca aagttggaga gctaggttta
    22261 gggcaacaac agttggttga aattgccaaa gcattgaata aacaggtacg gttgttgatt
    22321 ttagacgagc ctaccgcctc gttaaccgaa aaagaaaccg agattctgct gagtttaatt
    22381 aaagatttac aagctcacaa tattgcttgt gtttatattt cacacaaatt gaatgaagtg
    22441 aaagccattt ccgacaaaat ctgtgtcatt cgagacggcg aacatatcgg tactcgcagt
    22501 gcagagggaa tgagtgaaga cgatattatt acgatgatgg ttggacgtga gattacctcg
    22561 ctctacccac acgagccaca caatattggt gaagaagtcc ttcgagttga aaatttaacc
    22621 gcttggcacc caaccaatac acatatcaaa cgggttgata atgctaattt tgtactacgc
    22681 caaggtgaaa ttttaggtat tgctggtttg gtcggttcag ggcgaaccga gatggcacaa
    22741 tgtatttttg gctcttacca aggcaaatat caaggtgata tttttctgca taagcaaccg
    22801 attaaaatca aaaattgtgc acaagcgatt gaaaaccata ttgtgatggt gccggaagat
    22861 cgcaaaaaac acggcatcat tccaattatg ggggtgggta aaaacataac tctctcttca
    22921 ttaccgcaat tttgttttgg caaaaaagta ataaatgaac cgctggaaga aaccattatc
    22981 aaccaatcta ttgcaaaact gaaagtgaaa acttcctcgc ctgaattagc aattggacgg
    23041 ttaagtggcg gtaatcagca aaaagcaatt ttggcaaaat gcctattgct taaccctaaa
    23101 atcctgattt tagatgagcc gacccgtggt atcgacgtgg gagcaaaata tgaaatttat
    23161 aagctcatca accaattagc taaagaaggt atggcaatta tcgtcatttc ctccgaatta
    23221 cctgaagttt taggcattag tgatcgtgta ttggttatgc accaaggcaa aattaaagca
    23281 gatttgatta accaagacct tacccaagaa aaagtaatgg aagcggcact caaggagtaa
    23341 cctatgaata agttaaaatc gattaattta caagtttata ttatgctgat tgcaattgtt
    23401 gtgattatga gctttttctc tattgcaact gacggtgctt atttaagtgc cagaaatatc
    23461 tccaacctgt tacgccaaac ctccattacc ggtattcttg ccatcggtat ggtatttgtg
    23521 attatttccg cagaaattga tttatcggtt ggctcgctaa tgggcttact cggtggattt
    23581 gccgccatca gtaatgtatg gtggggctgg ccgctctcgt taactattat agttacttta
    23641 attttaggcg tcatgctggg tgcttggagc ggttggtggg ttgcttatca aaaagtgccg
    23701 tcctttattg taactcttgc cggtatgctg gcgtttcgtg gtgtattggt cggcttaacc
    23761 aacgggacaa ccgtttcacc gattagcaat gaaatgacaa ccattggtca aggctatttg
    23821 cctgatatgg tgggtatgat tttaggtact atcgggatat tttgctttat tgtttggggt
    23881 agctaccaac gcaaagccag acaaaaactc aatcttactg tgccttctat gtcgaaagac
    23941 acgctgaaat acggactagt tgcaattatc gtattaggtg cgatctacct actcaatgat
    24001 taccgtggtg tgccattccc ggtattggtg ttggtgattc tagccattgc cggcacattc
    24061 ttggccagaa aaaccgcctt cgggcgtcac atttatgcga ttggtggcaa cattgatgcc
    24121 gctcgcctct cggggatttc agttgaaaaa gtcaaacttg cgatttttgc cttaaacggc
    24181 ttattagttg ccattgcagg cttgatttta agctcacgtc ttggagccgg cgcaccttcc
    24241 gccgggcaaa atgccgaatt agatgcgatt gccgcctgcg tgattggtgg agccagttta
    24301 gccggcggta tcggtagcat ttatggcgta gttattggag catttattat tgctttgctg
    24361 gataacggta tgagtatgct tgatgtgcca accttctggc aatatatcgt taaaggtgga
    24421 attctactgc ttgcagtttg ggtagatacc atcagcaaga aaaaagccta attggataac
    24481 aagcggtcaa tctcgactat ccaacgggaa atccggcata atcgggctaa aaatgggaac
    24541 tattgtgcgt tttgccgtct ttttagcatt tgctacggca accagctcca ccaagtgcgg
    24601 tggcatttcg ccttgtttgg caaggttgca aaggtaggtt atcgctttaa tccggctcat
    24661 tgagccttcc agttccaaca catacgccac cagtgccatt cgtgcatcag caatttctcg
    24721 ctgtttggtg gtcaggtttc ccacatccac ctccgcacgc actgccggta actttttcgg
    24781 cttggcttcc accaccgccg acataaagcg ggagcggatt tcggtttgga tttcttgagg
    24841 gaggtttgag agtgcaaatt ctaaaatttc tcccccaact ccaacctttt tatgagtagt
    24901 ccatccttgt ttctttgcct gatacaaaac tccttgcttt gttttaggta aacaggctaa
    24961 acttaactca gccaattctt ttgccgaata gtgtgttttt agactgtttt cactcataaa
    25021 aacctcttta agctatctgt cggcaaattt tttatggttt cgttgagcat aacgttcagg
    25081 ccaaatttct tcgggtgata taccgattgc ttcggcaata atacgctcac cctttaacca
    25141 cggacgatct acagcgtttt tcaaggtgct ggattgactg tagccatgct tcaatgagag
    25201 ttgtctaagc gaccaacccg ctttatgtaa agcagcgatg atgtcggccc tatgccagtc
    25261 tttcgctgtt tttttaatat cttccaatgt actcattaga taactccttt cgcttaacga
    25321 tttagataat atacctattt ttactaaaaa taaagtgtaa aaaggttgct ttatagttga
    25381 tttatgagca aataaactta gtttgctgta agttattgat ttatttatag gtttttagag
    25441 tgtaaaaggc taaaaatctc aacttatttt tttacagtta tgaaaataga gtgtaaaaag
    25501 gttattggtt tcggtaaccg aatagaacag agcagaaaaa atgcaaaatt aagtagatct
    25561 gaggtttgtg atgccctagg aggtattgca atatctacat tacaagcgtg ggaggctgat
    25621 acaagagaac ctcctatatc aaaacttatg gctttgtcac aaatacttaa cgttgagcct
    25681 tgctacctat taacaggtga gcgacactcg caaaacctta atgtcacaag agagccacag
    25741 cctgtgtatg ccacaaaacc gcagacagaa gaaggggaag tagttgttat caaagctaag
    25801 ttacaagagg caatagaaac gttggaagag gtgctggaaa ttacccaacg ggaaatgaag
    25861 cctgccggaa aagcccaaat ggtgtgggct ttctacgagc ttctcacgca agaaaaaagc
    25921 gaaaaagaaa aaatggtggg attactcaaa ctggtggcat aacaggggac aatatggaca
    25981 aacaagccat tctcaaatta ttcagagaca atagcactgc cggcaacagc cagccaagcc
    26041 aagagcctat acaggtgttt aataacagca acgtcatcat tcaaaacggc gataacgcca
    26101 cggtgattgc cggctgcacg gtggataata acaccaacct aacctgtgcc gataaacaac
    26161 gtgtacacga cctcattcac agcttagagc aacgcaaccg agccagccga gatgagggcg
    26221 cttaaaattg ttttaaaatc cgtttaaacc tgctcagacc tatgcaaaaa ataacgtaaa
    26281 aatagcccat ttttttctat ttttgctcat tttcgttttt tgcataactc accccaaaca
    26341 aaaacggcga agccttgtat ttcctagcct cgccgttatt tttccgccca aatttttttc
    26401 tttctctcta tgcaaaaaag atcagtaccc cacactatgt tgccagacat actcaacaat
    26461 tttaccgtca acgtcagatt accaaaaatg accgcttgta atttagagga gtgtttagag
    26521 cggaaataac atcggaatca caaacaccga caccagcatc accagtacag taaacggcac
    26581 accaacttta acgaaatcaa taaatttata gcctgccggt gctaatacca tcgtatttac
    26641 cggtgaagaa accggcgtca taaaagcggc ggaggctgaa acagcgaccg tcatcacaaa
    26701 aggtgccggt gagtagccaa gttggttagc aatcgcaatg gctatcggca taaccaaaat
    26761 tgcagtcgct gtgttggaaa taaaagcact gataatcgct gttactacaa ataatactat
    26821 tagaacaaaa tgtacatcta agccttgcat tgcatcaagt agaagcctaa cggttaaatc
    26881 cacaccaccg gttttttgta aggcaataga aaacggcatc atccccacaa tcaacacaat
    26941 gcttggcata tgaatcgaat cataagcact tttcatatcc acacagcgga acttgcctaa
    27001 cattaagcag gcaatcaacg ctgccatcac gtttggcaca atgccggtaa tcatcagcaa
    27061 caccatagtc agcactgaga acagagcgtg aggggcttgg cttaacgccg gaggggcgtt
    27121 tttactttct gccggatctc taagcaagaa gaaatctcga tgatcgtcgt ccatacggtg
    27181 aatttgttgc catacgccca tcaccaataa aatatcgccg aatttgagtg gcgtatcgag
    27241 cagttcctct tgtatgattt catcaccacg ttttaagccg acaatgctta aatggtatct
    27301 tgagcggaat ttgagttccc gaatagtttt accgatacat tctgcttcag gcataaccga
    27361 aatttccgcc atacccaccg agcgatattg tgaggaaaag tattcgcctt ttagctcaat
    27421 cgggcgaagt ttgagctgtt cacagcgttg ttggtaagtt tcatcatcga gggcagtatc
    27481 aagcagtaaa atatctttcg gctggaaaat ttcgtttacc gaggcactga cggtaatttt
    27541 tctaaaccgc ttagtgcgtt gaattgcaat gatgtttagc ccttgctcaa tccgtaaatt
    27601 cagttgcccg atagatttgc cgacacagtc tgaatctgct tccaccagca ccattttggc
    27661 tcgctcttta agttgatact cttcaattaa ctgcgacatt gaaaattttt gagcctgact
    27721 gtgagtattg ctttgctcac cattatccaa ccaacggcgg gcaatcagca tatagccgat
    27781 gcccatcact aaaataatta atccaatcgg agtaaaactg aagaaattga agccttcaaa
    27841 acctgatttt actaattcgg aatgagcaac aaggttgggt ggtgtggcaa ttaaggtcat
    27901 cataccgctg ataagtccgg caacacttaa cggcatcatt aaccggcgag gagaaattcc
    27961 cattcccgag caaatggcaa gcaccaccgg aatgaaaatc gccacaatgc cggtggaact
    28021 cataaaagat ccaagcccgg caatacagag cattagcagc accagcactt tggtttcgct
    28081 cgctcccgac acccgcatca accaatcgct gacttggttc gccacgccgg ttcgcaccaa
    28141 accttctccg acaataaaga gcaacgcaat cagcaccacg tttgggtcac ttaagcctgc
    28201 caacacttct ttgactgata aaatattagt caggctaaaa aacagcatta caattaaggc
    28261 aaccacgtcc atacgcaact tgttttggat aaattgccaa atcgcaaata ccaataacgc
    28321 cattacccaa aacagttctt gcttaagaaa atccggcaca aaagagagca acgattgcat
    28381 aaccatatcc tccaaaatag ttagggatag attacaccgc tttaagcaaa gaaaaaagcc
    28441 gataagatca aaaagaaagg aaaaagcaat cacgtacggc gtgaatgctc agtacaagcg
    28501 gttagttttg acgaaatttt tacaatgggc tttccacatt cagttgttcg tcaatttcag
    28561 ttaccgccaa aatcaggctt gaacgtacca ttttttccaa ccgcatttgc tgcatttgaa
    28621 agagcatcga atctttattc gctgcactcg ctttcatatt cagcaattca ttgataggca
    28681 aaaaatcaat atggtgcaaa tctttggcaa atttcactac cgccgggtga gaaaatgcga
    28741 actcgtcttc ttcctcattc aaatgctgtt tgacttcaag gaaaagcgag atatcttcaa
    28801 acactttagg tgagatcacc cccaacccaa gcaacacttt taaacgtact tcaatgtcag
    28861 ccagcggtcc ttgatgttca aacaaggaat ccaccacgga ttttaacgca aagtccgttt
    28921 tgcgaaacac tcgttcaatc aactgttcaa ccgctttggc aaagcgttga ttggcaatgc
    28981 taaggaaacc acgcagggtg gtttcctcac tgagtttgtc gatgtaatcc atcatagtgt
    29041 caagaattat tgatataatt ccaccacttg agcaatgacc gcttggtcgg ttaaaccggt
    29101 gtatttttcc accgctgccg ccacaccgtg atttgcaata aattcggcaa gttcgaccgc
    29161 ttgtgggtct tcctcattgc gatattgcaa tgccatcacc acgcctttga ccaagttttg
    29221 atacggtaag ccatattcca acgtgccacg caacggtttg atcaagcggt cgttttcgct
    29281 cagtttgcgg attggctcac gccctacacg gtttacgtca tcgtttaagt acgggttggc
    29341 aaaacgtttg aggatttttt cgatatacgc tgaatgggct tgtgggtcga agccgtaacg
    29401 tttaatcagc acagcaccgc tctcttccat tgtggctttc acctgagttt tgatttcctc
    29461 aatcgcaatc gcctctttaa tccatttcac gcccgcttgt ttgcccaagt aggcacagat
    29521 taaatgcccg gtgttcaggg tgaacagttt gcgttccaca aacgccatca ggttatcggt
    29581 taattccatt cctttgatgt ttggaatgtc acctttgaac tgggtttgat ccacaatcca
    29641 ctcgctgaac tcttccaccg tgacttccaa cggatcagca gggttcggct cggcaggtgg
    29701 tacaattctg tccaccgcac tatcaacaaa accaaccact ttttcaattt ctgcttgctg
    29761 ctcggcagtt aaatgctcaa aaattttacc tttaaagaaa gttgtgccac gcaccatatt
    29821 ttcacaggcg ataatgttca gcggttgggt gttgccactt tccacacgag ccactaacgc
    29881 tttggcgaac agtggggcaa taaagccaag cacattcggg ccgactgcgg tggtaattaa
    29941 atcggtgttt ttcacttgct caatcacggc attttcgtct ttcgagttaa ttgccgcaat
    30001 gtttttgaca atttccacac ggctgtcatc gcccacgatt ttcacgccgt attgcttgtt
    30061 ttggttgatt tggtcgattt gagtttggtt aatatcggca aaagtcacga aaatgcgagc
    30121 atcagcaagc aatttgccga taaagccacg cccgatatta ccggcaccaa aatggagtgc
    30181 gttcattgat gattgttcct ctataaaacg taggggcggg tc
//
LOCUS       DANLRK010000009        28686 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.009, whole genome shotgun sequence.
ACCESSION   DANLRK010000009 DANLRK010000000
VERSION     DANLRK010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 28686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28686
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.009"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            99..1073
                     /gene="znuA"
                     /locus_tag="PXA33_000277"
     CDS             99..1073
                     /gene="znuA"
                     /locus_tag="PXA33_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HDL5119656.1"
                     /translation="MFKRTLIGLAVLGASVAQADVLTSIKPLGFIASAITEGVTETKV
                     LLPVTASPHDYSLKPSDVTQLQSAQLVVWVGDGLEGFLEKSIEKLPREKVLTLEDVDA
                     IDDIVDKASKKEDKDDDHDHKHEHKHEHKHEHKHEGHNHEGHNHDHHGHSHDEDWHIW
                     LSSEASELAAEQIAARLSEQMPEHKAKIAENLAKFKANLTAKKAEIAKQLEPVKAKGY
                     YTFHDAYGYFEREYGLNSLGSFTINPTVAPGAKTLTAIKKNIAKNKAQCLFAEPQFTP
                     KVIETLAKETKVNVGQLDPLGAKVQLSATAYPEFLQVLADEFSQCLTK"
     gene            complement(1125..1445)
                     /locus_tag="PXA33_000278"
     CDS             complement(1125..1445)
                     /locus_tag="PXA33_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005621693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnA domain-containing protein"
                     /protein_id="HDL5119657.1"
                     /translation="MIYPTCPECKSENTYHDSIQFVCPDCAYEWTGEETDQDDDVLIV
                     KDSNGNLLSDGDDVLLIKDLKLKGSSEVLKKGTKFKGIRLAQGDHNVDCGKIMLKSEF
                     LKKA"
     gene            1586..3949
                     /gene="rnr"
                     /locus_tag="PXA33_000279"
     CDS             1586..3949
                     /gene="rnr"
                     /locus_tag="PXA33_000279"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HDL5119658.1"
                     /translation="MIVDPNYQQELEKYENPVPSREFILNTIREHNAPMSRDELLSAF
                     HIDDEERMEGIRRRLRAMENDGELVFTKGKRYALPEKMDLIKGTVIGHRDGYGFLQVE
                     GHEKGATKSEDWFIPNAQMVKVMHGDFVLAQPNGTDRRGRKEVRIVRVLEARKKQIVG
                     RFFLESGIGFVVPDDSRVNQDILIPDEHRMGARMGQVVVVELQERKASFSRPVGIITE
                     ILGDNLAPGMEIEIALRNHDIPHIWPEGVEKQIRQFSSEEVPEEAKQGRVDLRDLPLI
                     TIDGEDARDFDDAVFAKKEGNGWRLWVAIADVSYYVRPKTALDLEALNRGNSVYFPNR
                     VVPMLPEVLSNGLCSLNPQVDRLCLVAEMSVSSKGELQDYQFYEAVMNSHARLTYTKV
                     WKMLEGDEALRERYAPLVPHIEELYAMFQTLMKARHKRGAIEFETIENQFIFNPQGRI
                     ERIEPLIRNDAHKLIEECMILANIAAARFVEKANEPALYRIHDKPSEEKLLSFKSFVR
                     ECGLTWDVGLDPTPKDYGVLLEQIAERADKELIQTMLLRSLKQAVYAADNIGHFGLAL
                     TEYAHFTSPIRRYPDLLLHRAIKYLIEKGKGNTRYYTDGGGYHYKLDDMDEFGDKCSA
                     TERRADEATREVADWLKCEFMQDHLGEEFDGVISSVTGFGLFVKLNELLIDGLVHIST
                     LDNDYYHYDADRQRLIGGSGIIYRLGDAVRVKVINVNLDEKKIDFELLTGNKKTGKTA
                     KKKAKATEVPVFKEPKTVKKAKKVAEKKPAKSAKKRTKTTASKSRKK"
     gene            3953..4219
                     /locus_tag="PXA33_000280"
     CDS             3953..4219
                     /locus_tag="PXA33_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5119659.1"
                     /translation="MDYKKQILFSLLFALVIAIISYFLFTYIPFDCSDLSKLNDSQRL
                     ECRTAEFLGVYYWLPHTTTLFFGSVLLYWAVYGMISLVQAVLKR"
     gene            4442..4900
                     /gene="mraZ"
                     /locus_tag="PXA33_000281"
     CDS             4442..4900
                     /gene="mraZ"
                     /locus_tag="PXA33_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HDL5119660.1"
                     /translation="MFRGASSISIDSKGRIAIPTRYRAELLEKHHGILVCTVDIRQPC
                     LLLYPLHEWEMVEQKLLALSNFDPVQRRIQRVMQGFATECEMDSAGRILLSPTLRQHA
                     QLEQQIMLVGQLNKFEIWQDKQWQAQIAEDLAFGSSAEILDCEALKNLSL"
     gene            4916..5854
                     /gene="rsmH"
                     /locus_tag="PXA33_000282"
     CDS             4916..5854
                     /gene="rsmH"
                     /locus_tag="PXA33_000282"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HDL5119661.1"
                     /translation="MSENPKHITVLLHEAVDGLAIKPNGIYIDGTFGRGGHSRLILSK
                     LGENGRLIATDRDPRAIAEAQTISDPRFQIEHTAFSAIPELCERMGLVGKIDGILLDL
                     GVSSPQLDEAERGFSFMRDGPLDMRMDTTKGLSAAEWLAQVSAEDLAWVLKEFGEERF
                     AKRIAQAVVSYNKSANEKISRTLQLAQIIADAVPFKDKHKHPATRSFQAIRIFINGEL
                     DELEKALNSALTVLAPEGRLSIISFHSLEDRMVKQFVRKHSKGMDVPRGLPILEAELT
                     KNIPLKTIGKAIMPSEAEIEANARSRSAVLRIAEKR"
     gene            5873..6184
                     /gene="ftsL"
                     /locus_tag="PXA33_000283"
     CDS             5873..6184
                     /gene="ftsL"
                     /locus_tag="PXA33_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HDL5119662.1"
                     /translation="MSNERYPLHQIIIDDIFSHNKVALLLLIGVVISAVATIWITHQT
                     RLSVSEQGQLIQANQKLESQYTNLQLEENSRSSRARVDAAAKSFGLQPIKKEQEIILV
                     E"
     gene            6188..8248
                     /locus_tag="PXA33_000284"
     CDS             6188..8248
                     /locus_tag="PXA33_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005622210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsI"
                     /protein_id="HDL5119663.1"
                     /translation="MVKSVKLKRKQEAPVAKAEPQQAEGKKTPSFLPTRFRVVVFFML
                     LMVGGLLAQTAYIQLFDSERLIKEANNRSLRTKELQFTRGRILDRNGRFLSISVPMYS
                     LTLDPREYFDTKLRRSNESWRALAIEMETSKGKIEANVNKFIEKKNFSKEKVDFDPRS
                     ILNTKSEGYWTQLAQVTGLDYNSLIEKVRNNPSSVFLRLDNENLVRERQKFQALSKEI
                     GRSYNDIMDELYSKNRQRFLYLSRHQSEAISEYAKELDIDALVIKVESRRFYPLAEES
                     SQLIGFTDKDDMHGSEGLERSFDSLLIGKNGKQIIRKDAKGNIVENIRSEKQYDPQDV
                     MLSIDEELQSMVYGEIKKAVQENNAESGTAVLVDVQTGEILAMANAPSFNPNKRDSFK
                     PELMRNRAITDTFEPGSTVKPFTVLTALQNGVTYRDEVIGTRPFVVNGHTIKDVAPRD
                     SLTLTGILQKSSNIGVSKLALRMPANALVDTYTKVGFGKDTGLGLGEQRGTNGDRKRW
                     ADIERATLSYGYGLNVTPLQLARAYATLGSFGLYRPLSITKVDPPVIGERVLPEKTTR
                     DVVHMMESVAAKGEGGHRAAVDGYRVAIKTGTARKLEKGKYVEKYIAYTAGLAPASDP
                     RFALVVLINEPKAGNYYGGFVSAPLFSRIMGYTLKQRNIKPDNLTENTQSAVREIKLE
                     RPIN"
     gene            8257..9747
                     /gene="murE"
                     /locus_tag="PXA33_000285"
     CDS             8257..9747
                     /gene="murE"
                     /locus_tag="PXA33_000285"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HDL5119664.1"
                     /translation="MKRLLPFLTELDAWVEELTELKQMTLDSRQVQAGDLFVALKGHQ
                     VDGRKFIPNAIEQGAAIILAEADEDQPEIELDPKFAKFNLDRTACCKVVSVPRLPKLL
                     SEIAGALYHNPSHKLTLAGITGTNGKTTTAQLLAQWHNLLGGKSAVMGTIGNGLYGQV
                     QEAVNTTGSAIEIQHNLANFVELGADFCAMEVSSHGLAQFRAEALDFDLAIFTNLSRD
                     HLDYHNTMEEYAQAKFRLFNELSTKAQVINADDEIGREWLTQLPNAVAVSTDPKFAGN
                     HQFVKATAVKFTLQGASIAFESSWGNGELHSRLIGAFNVNNLLTAFAGLLALGFDIQE
                     LVKTAPNLVGVAGRMECITAPNKPMVIVDYAHTPDALEKALQAARLHCEGELWCIFGC
                     GGDRDAGKRPLMATIAEKLTDKVIATDDNPRTEDNQKIMADIVKGFSKPQQIIHNREE
                     AIKTAIEQAKATDVILIAGKGHEDYQIIGTEKLHFSDQETARKYLF"
     gene            9835..11229
                     /gene="murF"
                     /locus_tag="PXA33_000286"
     CDS             9835..11229
                     /gene="murF"
                     /locus_tag="PXA33_000286"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HDL5119665.1"
                     /translation="MIKLTTQQIAEILNAKLIGNGETVVETTSTDTRQAVENGLFFAL
                     KGENFDAHHYLQNAVAQGCVAVVVERECEIDVPQLVVADTRLALGELAKWLKAKLNPK
                     TVAMTGSSGKTTVKEMTAKILQKMTACEQEVLYTFGNLNNDLGVPMTLLRLTEQHKYA
                     VIELGANHIGEIAYTTAIAQPDACLVNNVAAAHLEGFGSLAGVAQAKGEIYRGLKENG
                     KAIVNLAFYYPQWQKEIGSREMQSFSYIGSDSDSYADYWAEDVELHLSGSRFTLHTPQ
                     GEIAIDLPYLGKHNVSNALAATSLAMSVGATLEAVKAGLEQRSMVKGRLYPVQINDHC
                     LFIDDTYNANVDSMKSAISVLENYPAYRIFAVGDMAELGEESSKCHQEVADFAKNAAL
                     DLIVSFGKESAVISQQAEHHFTDKQQMVDFLLPIILQKIEQKQPLVLLAKGSRSQKME
                     TVISELCKPFNLNF"
     gene            11250..12332
                     /gene="mraY"
                     /locus_tag="PXA33_000287"
     CDS             11250..12332
                     /gene="mraY"
                     /locus_tag="PXA33_000287"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HDL5119666.1"
                     /translation="MLVWLAELLVQYNTAFNVVSYITFRAIMALLTAMGIGLWIGPKV
                     IRRLQILKFGQEVRNDGPESHFKKRGTPTMGGVMILAAIGVSTLLWADLRNSYVWFTL
                     FVLFGYGAVGFVDDYRKISRKNTDGLIARWKYFWLSAIALVAVFGMYAVGKDTAATQL
                     VVPFFKDVMPQLGLFYIVLSYFVIVGTSNAVNLTDGLDGLAIVPTIMVASAFALIAWA
                     TGNFNFAQYLHIPFIPNAGELVILCTAIVGAGLGFLWYNTYPAQVFMGDVGSLSLGGA
                     LGVIAVLVRQELLLVVMGGVFVVEALSVILQVGSYKLRQKRIFRMAPIHHHFELKGWS
                     EPRVIVRFWIITLMLVLVGLVTLKLR"
     gene            12396..13697
                     /gene="murD"
                     /locus_tag="PXA33_000288"
     CDS             12396..13697
                     /gene="murD"
                     /locus_tag="PXA33_000288"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005606684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HDL5119667.1"
                     /translation="MQKQYENKTVTIIGLGTTGLSCVDFFKKKNANIQVIDTRANPAG
                     ADKLDVTIPLHTGGLNSEWLLKSDLIVISPGLAVATPEIQQAIQAGVEIVGDVELFCR
                     EAKAPIIAITGSNGKSTVTTLTTEMAKQAGIKVGMGGNIGVPVLSLLEGDYDLFVLEL
                     SSFQLETTYSLKAKAATVLNISEDHMDRYDSIADYRQAKLRIYDNAENIIVNGEDAQT
                     YPIQAVKKVIRFAEQNAEYSLRNQMLYSGDDAIIQTKQMLISGRHNEMNALAAMALAE
                     AANVPREGIVKALQIYGGLPHRFQAVATNDGVRWVNDSKATNVGSTVAALNGLNIAGT
                     LYLLLGGDGKAADFSELKPLINKPNIVCYCFGQDGAALAELSEQSVLVETMQQAIEAI
                     RPQLKKGDMVLLSPACASLDQFKNFEQRGEVFMALAQGQHA"
     gene            13697..14875
                     /gene="ftsW"
                     /locus_tag="PXA33_000289"
     CDS             13697..14875
                     /gene="ftsW"
                     /locus_tag="PXA33_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HDL5119668.1"
                     /translation="MLERIKSEWDKWVRLTPSNSLYDRTLIWLFLGLLIIGFVMVTSA
                     SLPVSTRLNNDPFYFAIRDGLYIIASIIFCYVFVQIPIEKWEKHNLALFFISIGFLIA
                     VLIFGRSINGAVRWIPLGILNFQPAELAKLAVICYFASFYVRKYDEIRKEKASFWRPA
                     VILFLFGFLLILQPDLGSTFVLFVLTFSMLFIVGAKIMQFMFLGVVGTVLFAVLILTS
                     EYRLKRVTSFMDPFADAYGDGFQLSNSQMAFGQGEFWGQGLGNSVQKLEYLPEAHTDF
                     VMAVIGEEFGFFGIACIVLLLILLTVRALKISKESLVLEERFKGYMAFGIAIWVFLQG
                     FVNLGVASGLLPTKGLTFPLVSYGGSSLVIMSIAIAVLLRIDHENRAERIGHAKIKTI
                     "
     gene            14994..16049
                     /gene="murG"
                     /locus_tag="PXA33_000290"
     CDS             14994..16049
                     /gene="murG"
                     /locus_tag="PXA33_000290"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDL5119669.1"
                     /translation="MAKKLLVMAGGTGGHVFPAIAVARELQKQGWQIRWLGTKDRMEA
                     TLVPKHGIEIDFIEISGLRGKGVAALLKAPFAIFKAVMQARKIIKNYQPDAVLGMGGY
                     VSGPGGIAAKLCGVPVILHEQNAVAGLTNVWLSKIARKVLQAFPTAFKEAEVVGNPVR
                     EDLSALPLPNERFAKRGYPINILVMGGSQGARVINQTVPEVAKVLGNNVFISHQVGKG
                     NLAGIEEVYQQTGNGIASEFIDDMKAAYEWADLVICRSGALTVCEIAAVGLPAVFVPF
                     QHKDRQQFLNANYLAADGAAVIIEQSDFTPKALQQALEPLIANRQLLLDMSMRARAKS
                     TPMAAKRVAEVIIQEAN"
     gene            16125..17552
                     /gene="murC"
                     /locus_tag="PXA33_000291"
     CDS             16125..17552
                     /gene="murC"
                     /locus_tag="PXA33_000291"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013527829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HDL5119670.1"
                     /translation="MKNFQDKVKKLVPEMRRVSQIHFIGIGGAGMSGIAEVLLNEGYQ
                     ISGSDIADRAVTQRLSAAGAKVFIGHQAENIAGASVVVVSTAIDETNPEIIAAREARI
                     PVIRRAEMLAEIMRFRHGIAIAGTHGKTTTTAMISMIYTEAKLDPTFVNGGLVKSTGK
                     NAHLGASRYLIAEADESDASFLHLQPMVSVVTNIEPDHMDTYGGDFEKMKETYVRFLR
                     NLPFYGLAVMCADDETVMEIAPRVGRQVITYGFSENADYRIEDYQQTGFQEHYTVVCP
                     SGERIDVLLNVPGMHNALNATAALAVAKEEGIGNEAILAALADFQGAGRRFDQLGSFI
                     RPNGKIMLVDDYGHHPTEVDVTIQAARSGWEGKRVVMIFQPHRYSRTRDLFDEFVQVL
                     SKVDALILLDVYAAGEAPIVGADSKALARSIRNLGKVDPILVSDTDQLGEVLDQIIQD
                     GDLILAQGAGSVSRISRGLAESWKN"
     gene            17642..18553
                     /locus_tag="PXA33_000292"
     CDS             17642..18553
                     /locus_tag="PXA33_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HDL5119671.1"
                     /translation="MSIKNEKIAVLFGGVSQEREVSLNSGAAVTAALKSLGYNVEGID
                     TKEFPIEKLKEKGIQRVFNILHGGIGENGVLQGALEQMGIPYTGCGVMASAVTLDKFR
                     TKLLWNAVGLPTADMVVVQRGQAVDFDQIIAKLGLPVFVKSSCEGSSVGVFKVKTKEE
                     LAPAIHEALKFDSIVLVEEFLAGNEYSVPVLDGQVLPAVQVIPDGEFYDYHAKYVSDN
                     TQYVVPALNDERQAEVAKLVKAAYDVVGCRGWSRIDVMEDGEGKFRLVEVNTNPGMTS
                     HSIFPKSAATVGISFEKLVERVLELSA"
     gene            18553..19344
                     /locus_tag="PXA33_000293"
     CDS             18553..19344
                     /locus_tag="PXA33_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HDL5119672.1"
                     /translation="MGIFRKKNSTNVRLNVLNPKTPVSPRKWLVFIKPLIVLLCTLFV
                     FGVYSNWQNILESWDKTPIRAYALTHKTQFTTNADIRETLSKEPALKGYFGQNIQEVK
                     DKLLSIPWVKDVAVRKLYPDRLSITLLEHKPVAIWNDTNFVSEQGTVFSLPKDRIDKN
                     GLPLLYGPDTEGKSVLEAWNKIRADLNARNLDLTSVSVDNRGSWTIRLSNQVELRLGR
                     GEWTSKIDRFVTIFPEIHIPEGNRLAYVDLRYPHGAAVGFSPIKN"
     gene            19367..20638
                     /gene="ftsA"
                     /locus_tag="PXA33_000294"
     CDS             19367..20638
                     /gene="ftsA"
                     /locus_tag="PXA33_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HDL5119673.1"
                     /translation="MTKAVESKIIVGLDIGTSKVVAVVGEVLPDGVINVMGAGVCPSK
                     GVDRGGVIDLDAAVNSIQRAIEQAESIADCQIMGVTLAISGQHIVGLNEAGSSTLSGG
                     IVTQDNIESAVDMAKAIKLQDGLETLHMIPQEYHVDRLPATKNPIGLSGMRLQVQAHL
                     IACHQAWLKNLKNAVEHAKLKVDQVVFSGLASSYSVLTEDEKELGVCLIDIGGGTMDV
                     LVYTDGALRYSKVIPFAGNNITDYLARVFTTSRPEAESLKVGYGSAISPPTHNSDKKI
                     EVAGLGGRMARTFTRAQVATVTSQCYNDLLKVVEEELTQLRHELFQKGIKQELIAGVV
                     ITGGGSQIEDIVECAKSIFGSQVRVGYPLNITGLTDYVNKPQYATALGLLQYSHYNYT
                     EQGTSTDSNEDIFAPIGRGTKKIIDFFKSKF"
     gene            20671..21918
                     /gene="ftsZ"
                     /locus_tag="PXA33_000295"
     CDS             20671..21918
                     /gene="ftsZ"
                     /locus_tag="PXA33_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HDL5119674.1"
                     /translation="MFEPIIEQTQDAIIKVVGVGGGGGNAVDRMSRSADDIKGVEFFD
                     VNTDAQVLRKRTTRQTIQIGASTTKGLGAGADPMVGKQAAEEDREAIANALKGANMTF
                     IAVGMGGGTGTGAAPVVAQIAKEQGSLTVGVVTKPFRFEGPRRMRFADQGIKELSQYV
                     DSLIIIPNDKLRGLGKQTTAVDAFAAANDVLSNCVLGITNMITSSGGSTGADINVDFA
                     DVRTVMSGKGHAMIGTGFAEGEVGEGRAEKAMNDAISSPLLENVDISGASGMLINISA
                     GTDFLLEEVYAMMDLIYGFATEDAAIVFGCNYYPEMDGKVSVTLVATGIGQPEEALHM
                     PHKAQPVYAQQGNHVQATQPTQPNHAVQQPNFTQPSSYGQPNQFSGQPQPLQQPVAPK
                     PQSVDTAQIFNPNSIPGFMRNSQ"
     gene            21950..22870
                     /gene="lpxC"
                     /locus_tag="PXA33_000296"
     CDS             21950..22870
                     /gene="lpxC"
                     /locus_tag="PXA33_000296"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012073651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HDL5119675.1"
                     /translation="MTKQRTLKQAAKVTGIGLHSGKKVTLTLRPAPANTGVIYARTDL
                     EPVVYFPASADSIRDTQLCTCMVNDDGVRISTVEHLNAAMSALGLDNVIVEVDAPEIP
                     IMDGSSSPFIYLLLDAGIEEQDAPKKFIRIKETVRVEEGDKWAELKPYNKGFKLNFTI
                     DFEHPLIPKNVGSYSIDFSAENFIQQLSRARTFTFMKDVEYLQSIGLALGGSLDNAIV
                     LDDYRILNEDGLRFKDELVRHKMLDSIGDLYMCGYNILGEFSAYKSGHGVNNKLLRAV
                     LENKNAWEFVTFEDKAEVPQGYQVQQEIFI"
     gene            23043..24725
                     /gene="recN"
                     /locus_tag="PXA33_000297"
     CDS             23043..24725
                     /gene="recN"
                     /locus_tag="PXA33_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HDL5119676.1"
                     /translation="MLTQLTINNFAIVRHLILELNEGMSVITGETGAGKSIAIDALGL
                     CLGYRSESGMIRNGSDKADISATFTMQPHSPAYLWLQEHELLDEDNPQECILRRMINI
                     DGRSKAFVNNRSLPVSQLRELGQYLIHLNGQHAPQLLLKNEYQLEIVDHYATLQPLLM
                     KMAKQYGNWKELNKQVKNFQKQCQENEARKQLLEYQVEELDEFAIKANEFEEMEEKYN
                     ILSSSEELTELSDEITRLLSDDEINANSLIYKALRNAENLAEVDSQYQGILDMLNEAL
                     IQIQEASSEVAQLASKIEQDPELLQELDNRIAQTISLARKHHIQPNELWQYHATLKAE
                     LQELLDFEDNEEQLIQNEKQAYQECITLAEEIYQKRLDATHKLSEQVTKQIKHLAMEN
                     GEFFIHIEHNLEKMSANGADYVEFNLRSNLGQQPQPLAKIASGGELSRISLAVQVLTA
                     NKLSTPTIIFDEVDVGISGSAATTVGKLLRKLGEKCQVLCVTHLPQVASYGNQQFNVQ
                     KYVENNQTETQMTLLTEAERIHALARLLGGSEITETVLANAKEMLNLAKNNE"
     gene            complement(24772..24847)
                     /locus_tag="PXA33_000298"
     tRNA            complement(24772..24847)
                     /locus_tag="PXA33_000298"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24812..24814),aa:Ala,seq:ggc)
     gene            complement(24927..26366)
                     /gene="gltX"
                     /locus_tag="PXA33_000299"
     CDS             complement(24927..26366)
                     /gene="gltX"
                     /locus_tag="PXA33_000299"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007526628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HDL5119677.1"
                     /translation="MKIETLFPLDPNVKVRTRFAPSPTGYLHVGGARTALYSWLYAKH
                     NDGEFVLRIEDTDLERSTPEATAAILEGMEWLDLAWEHGPYFQTKRFDRYNQVIDQML
                     EAGTAYRCYCSKERLDEVRAEQEANKEKAKYDQHCLHNPQNDPSQPHVVRFKNPTEGS
                     VVFDDAVRGRIEIANSELDDLIIRRTDGSPTYNFCVVVDDWDMGITHVVRGEDHINNT
                     PRQINILKALGAPIPTYAHVSMINGDDGQKLSKRHGAVSVMQYRDDGYLPEALINYLV
                     RLGWGHGDQEIFSRDEMIELFDIHSVSKSASAFNTEKLQWLNQHYIRSLAPSYVAKHL
                     EWHMQTQGIDFTSGPKLEEIIPVLGERCKTLKELAESSRYFYQEFKAYDEKAAAKNFK
                     AEAVAPLAKLLEKLTACNDWTVENIHEAMNQTAAELEIGMGKVGMPFRLAVTGSGQSP
                     SMDITAKLVGKERTLARLQKAIEFIQANS"
     gene            26541..26616
                     /locus_tag="PXA33_000300"
     tRNA            26541..26616
                     /locus_tag="PXA33_000300"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26574..26576,aa:Val,seq:tac)
     gene            26665..26740
                     /locus_tag="PXA33_000301"
     tRNA            26665..26740
                     /locus_tag="PXA33_000301"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26698..26700,aa:Val,seq:tac)
     gene            26793..26868
                     /locus_tag="PXA33_000302"
     tRNA            26793..26868
                     /locus_tag="PXA33_000302"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26826..26828,aa:Val,seq:tac)
     gene            26917..26992
                     /locus_tag="PXA33_000303"
     tRNA            26917..26992
                     /locus_tag="PXA33_000303"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26950..26952,aa:Val,seq:tac)
     gene            complement(27146..28639)
                     /gene="mltF"
                     /locus_tag="PXA33_000304"
     CDS             complement(27146..28639)
                     /gene="mltF"
                     /locus_tag="PXA33_000304"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HDL5119678.1"
                     /translation="MKGLIARLFIAVALLLWAWDMIFPWQKIIHSEANHYTQIQQSKT
                     LKVGMINHPLSYFVGPEGKSGIEYDLATAFAQYLKVNLDIKTYDSSEKLFEALRNNDV
                     DIAAAGLLYHSELNDEFQIGPSYYSASWQLVYKKGSARPYRLSDLRENVIIPAGSAVI
                     PILNQLKVDNPELKWQISDKFTQEELLLKVAQGEISHTIAMSVDISAAQHINPNLAVG
                     FDLTDENPVLWYLANSSYSELQSVLLDFMSYSMETGLISRIEEKYFNHLAKFDYVDIQ
                     SYLNAIKTILPKYEPLFRAHKGELEWQMLAAIAYQESHWDPNATSPTGVRGIMMLTKD
                     TADRMKISDRTNPAQSIKAGSEYLHMLMRQMPETIPQADRIWYSLAAYNMGLGHLLDL
                     RRLTKQLGGDPDNWLDVKKNLPLLAEKRYYANLKYGYARGYEALQYVENIRRYYGSII
                     NYQRVEEQKMQEQENLPDGQIKQENSSYEKIEEKEIPTDKEITPASN"
BASE COUNT         8707 a         5515 c         6397 g         8067 t
ORIGIN      
        1 aaaacgtaaa tgaaaaatac ccataaaaaa tttatagaaa ttttgtttta ttttgttaga
       61 atagctcggt tcaaattaaa tgccataagg ggaattttat gtttaaaaga acattaatcg
      121 gtctagccgt gttaggtgca agtgttgctc aagcggatgt attaaccagt attaagcctt
      181 taggctttat tgccagtgcc attaccgaag gggtaactga aactaaagta ttattaccgg
      241 taactgcctc tccacacgat tacagcttaa aaccttcaga tgtgactcaa ttacaatctg
      301 ctcaattagt ggtttgggtg ggtgacggct tggaaggctt tttagaaaaa agtattgaaa
      361 aactgccgag agaaaaagta ttaacccttg aagatgttga tgcgattgat gatattgtcg
      421 ataaagcaag caaaaaagaa gataaagacg atgatcacga tcataaacat gaacataagc
      481 acgaacacaa gcacgagcat aaacacgaag ggcataatca cgaaggacat aaccacgatc
      541 atcacggaca tagccacgat gaagattggc atatttggct ctcaagcgag gcaagtgagc
      601 tggcagcaga gcaaattgcg gcacgtttaa gcgaacaaat gcctgaacac aaagcgaaaa
      661 ttgcggaaaa tcttgcaaaa tttaaagcaa atctaaccgc taaaaaagcc gaaattgcga
      721 aacaattaga gccggtaaaa gccaaaggtt actacacatt ccacgatgct tacggctatt
      781 ttgaaagaga atatggttta aattcattgg gttcatttac cattaaccca actgttgccc
      841 cgggtgcaaa aacacttaca gcaatcaaga aaaacattgc gaaaaacaaa gcacaatgtt
      901 tatttgccga gccgcaattt acgccgaaag taattgaaac cttagcaaaa gaaactaaag
      961 taaatgttgg ccaattagac ccactcggtg caaaagttca gttaagtgcg actgcttacc
     1021 ctgaattttt acaagttttg gctgacgaat ttagccaatg tttaacaaaa taagttaaaa
     1081 atcccccatt taaatataaa tgggggattt tgcgattaca tcacttaagc tttttttaag
     1141 aattctgatt tcaacataat tttaccgcaa tcgacattat ggtcaccttg agctaaacgg
     1201 attcctttga attttgtacc ttttttcagt acttcagacg agccttttaa ttttaaatct
     1261 ttgattaata atacatcgtc cccgtcagat aataaattgc cattactgtc ttttacaatt
     1321 agcacatcat catcttgatc cgtttcttcg cccgtccatt catacgcaca atccgggcaa
     1381 acaaattgaa ttgagtcgtg gtaagtattt tcgcttttac attcagggca ggttgggtaa
     1441 atcattttaa ttcctcaaat taataaagaa ccagattata taccgaaatc cagcaaaacg
     1501 ctaagcggtc atattttgcc gattttttac caatcgagct aaaagtcagt tagaatagcc
     1561 ttaattacac aaaaaggagg atactatgat agtcgatccg aattatcagc aagaattaga
     1621 aaaatacgaa aatccggtac caagccgaga gtttatttta aatacaattc gtgaacataa
     1681 cgccccaatg agccgtgatg aattattgtc ggcttttcat attgacgatg aagaacgaat
     1741 ggaaggcatt cgccgccgtc tgcgtgcaat ggaaaatgac ggtgaattgg tgttcaccaa
     1801 aggcaaacgc tatgcgttgc cggaaaaaat ggatttaatc aaaggtacag ttatcggcca
     1861 tcgtgacggt tacggttttt tgcaagtgga aggacacgaa aaaggggcga ctaaaagcga
     1921 agattggttt attcccaacg cccaaatggt aaaggtaatg cacggtgact ttgtgctggc
     1981 acaaccaaac ggcacagacc gcagaggtcg gaaagaagtc cgcattgtgc gagtgttgga
     2041 ggcccgtaag aaacagattg tcgggcgttt tttccttgaa agcggtatcg gttttgttgt
     2101 gcctgatgac agccgcgtta accaagatat tctcattccg gatgaacacc gtatgggggc
     2161 gagaatgggg caagttgtgg tggtggaatt acaggaaaga aaagcgagtt ttagccgccc
     2221 ggtgggaatc attactgaaa ttctaggcga taatttagcg ccgggaatgg aaattgaaat
     2281 tgccctacgc aatcacgata ttccgcacat ttggccggaa ggggtagaaa aacaaatccg
     2341 ccaatttagt agcgaagaag tgccggaaga agccaagcaa gggcgggtgg atttgcgaga
     2401 tctgccactg attaccattg acggcgaaga tgcccgagat ttcgatgatg ctgtatttgc
     2461 caaaaaagag ggcaacggct ggcggttatg ggtggcgatt gccgatgtaa gttattatgt
     2521 tcgcccaaaa accgcattag atttggaagc cctaaatcgt ggtaactcgg tctatttccc
     2581 gaatcgggtt gtgccaatgt tgccggaagt gctttccaat ggcttgtgtt cactcaatcc
     2641 acaggttgac aggctctgct tggtggcgga aatgtcggtt tccagcaaag gggaattaca
     2701 agattatcaa ttctacgaag cggtaatgaa ctctcacgcc cgtttaactt acactaaagt
     2761 gtggaaaatg ttggaaggtg atgaagcctt gcgtgaacgc tatgctccat tagtgccgca
     2821 tattgaagaa ctctatgcaa tgttccaaac cttaatgaaa gctcgccata aacgaggggc
     2881 gattgaattt gaaaccattg aaaatcaatt tattttcaat ccgcaagggc gaattgagcg
     2941 aattgagccg ttaatccgca acgatgctca taaattgatt gaagaatgta tgattttagc
     3001 caatattgct gccgctcgtt ttgtggaaaa agcgaacgaa ccggcgttat atcggattca
     3061 cgataagccg agtgaagaaa aattgctcag ttttaaatcc tttgtgcgtg agtgtggctt
     3121 aacctgggac gtaggcttag atccaacccc aaaagattac ggtgtactac tggagcaaat
     3181 tgccgaacgt gccgataaag agttgattca aacgatgttg ctccgctcgc tgaaacaagc
     3241 agtgtatgcg gcggataata tcgggcattt cggcttggca ttaaccgaat atgctcattt
     3301 tacctcgccg attcgccgtt acccggattt actgttacac cgtgcaatta aatatctgat
     3361 cgaaaaaggc aaaggcaaca ctcgctacta taccgatggc ggtggttatc actacaagtt
     3421 agatgatatg gacgaattcg gcgataaatg ttctgccacc gaacgccgag ctgatgaggc
     3481 aacccgagaa gtggcagatt ggctgaaatg cgaatttatg caagatcatc ttggcgaaga
     3541 gtttgatggc gtgatttcga gtgttaccgg ctttggctta tttgtaaaac tcaatgagct
     3601 tttaattgac ggattggttc atatttcaac gctggataat gattattacc actatgatgc
     3661 ggaccgacaa cgtttaatcg gtggcagtgg gatcatctat cgtttaggcg atgcagttag
     3721 agtaaaagta atcaatgtga atttagacga gaaaaaaatt gattttgagt tactcaccgg
     3781 taataagaaa accggcaaaa ccgccaagaa aaaagcaaaa gcgacagagg ttcctgtttt
     3841 caaagagcct aaaacggtaa aaaaagccaa aaaagtcgct gagaaaaaac cggcaaaatc
     3901 tgcaaaaaaa cggacaaaaa cgaccgctag taaatcaagg aaaaaatagc aaatggatta
     3961 caaaaaacaa attttattca gccttttatt tgctttagta attgcaataa taagctattt
     4021 tttgtttacc tatatcccgt ttgattgtag cgatctgagc aaactcaacg attcacaacg
     4081 tttagagtgc cgaactgccg aatttctggg cgtttattac tggctgccgc acactaccac
     4141 gctgtttttc ggctcggtgt tattatattg ggcggtttat ggcatgattt cattagtgca
     4201 agcagtgctc aaacgctaac aagcagtttt gtttttgcaa aaagttgcaa atctcatctg
     4261 aataatcctt ccaagatcaa aggtctatca aaaaagatag acctttattg tgtaaataaa
     4321 ctaaaactcc ttgatagatg tatcacatat ctataaaatt ccagcaatta agtgggaaaa
     4381 agtggtaaat tgtggaactt ttccttaaat ttaacctcat ttaaaaaggt aaatcagtaa
     4441 aatgtttcgt ggtgcaagtt caatcagcat tgatagcaaa gggcggatcg ctattccgac
     4501 ccgttatcgt gccgagctat tggaaaaaca tcacgggatc ttagtttgca ctgtggatat
     4561 ccgccagcct tgtttgttgc tttacccact tcacgaatgg gaaatggttg aacagaaatt
     4621 gttggcacta tctaattttg accctgttca acgccgtatt cagcgagtaa tgcaaggctt
     4681 tgctaccgaa tgtgaaatgg attcggcagg gcgaatactg cttagcccta cacttcgcca
     4741 acacgcacaa ttagagcaac aaattatgct ggttggacaa ttaaacaaat ttgaaatttg
     4801 gcaagataaa caatggcaag cacaaattgc cgaagatctt gcttttggca gttcggcaga
     4861 aattttagac tgcgaagcat tgaaaaacct gtctctctag taattggaaa ttgagatgag
     4921 cgagaaccca aaacatatta cagtattatt gcacgaagcg gttgacggct tggcaattaa
     4981 gcctaacggt atctatattg acggtacttt cgggcgtggc gggcattctc gcttgatttt
     5041 atccaaactg ggcgaaaatg ggcgattaat cgcaactgat cgtgacccac gagcgatagc
     5101 cgaagctcaa acgattagtg accctcgttt tcaaattgaa catacggcat tttctgccat
     5161 tcccgaactg tgtgaacgaa tggggttggt gggcaaaatt gacggtattt tattggattt
     5221 aggtgtgtct tccccgcagc ttgacgaagc cgaacgaggc tttagtttta tgcgtgacgg
     5281 gccgcttgat atgcgaatgg ataccaccaa aggcttatcg gcagccgagt ggcttgccca
     5341 agtttcggca gaagatttag cttgggtatt gaaagaattt ggcgaagagc gttttgccaa
     5401 acggattgca caagcggtcg tttcttacaa taaatctgca aatgaaaaaa tcagccgcac
     5461 tttgcagctt gcgcaaatta ttgcggatgc ggtgccgttt aaagataagc acaaacaccc
     5521 tgcaacccgt tcgttccaag caattcgcat ttttatcaat ggtgaattag acgagctgga
     5581 aaaagcttta aattccgcat taacggtgtt agcccctgaa gggcgtttgt cgattatcag
     5641 ctttcattct ctagaagacc gaatggtgaa acagtttgtg cgtaagcata gtaaaggaat
     5701 ggacgtaccc cgtggtttac cgattttaga agcagagcta accaaaaata ttccgctgaa
     5761 aaccattggc aaagcgatta tgccgagcga agccgaaatt gaagcaaatg cccgctcacg
     5821 cagtgcggta ttgcggattg ccgaaaaacg ctaacagtaa atagggaaga atatgtcgaa
     5881 cgaacgttat ccgttacatc aaatcatcat tgatgatatc tttagccata acaaagtggc
     5941 tcttttattg ttgattggtg tagtgatttc agcagtggct acgatttgga taacccacca
     6001 gactcgttta tcggtgagcg aacaagggca actgattcaa gcaaaccaaa agttggaaag
     6061 ccaatatact aatctgcaat tagaagaaaa cagcagaagc agtcgggcaa gagttgatgc
     6121 cgccgctaaa tcgttcggat tacagccgat taaaaaagag caagaaatta ttttagttga
     6181 ataaaaaatg gttaaatcag ttaaattaaa acgtaagcaa gaagcccctg tcgcaaaagc
     6241 agagcctcaa caagcagaag gtaaaaaaac accgagtttt ttacctactc gctttagagt
     6301 ggttgtgttt tttatgctat taatggttgg tggcttacta gcacaaaccg cctatattca
     6361 gctttttgac tctgaaagat taattaaaga ggctaataac cgctcacttc gtactaaaga
     6421 actccaattt acccgtggac gtattttaga tcgtaacggg cgttttctct ctattagtgt
     6481 gccaatgtac tcattaacac ttgacccgag agaatacttt gatactaagc tgcgtcgttc
     6541 taacgaaagc tggcgtgcct tagcgattga aatggaaaca tcaaaaggta aaattgaggc
     6601 aaacgttaat aaatttattg aaaagaaaaa tttttcaaaa gaaaaagtag attttgatcc
     6661 acgttctatt ttaaatacta aaagtgaagg ttattggact cagcttgccc aagttacagg
     6721 tttggattac aacagtttaa tagaaaaagt acggaataac cctagctcgg tttttttacg
     6781 tttagataac gaaaatttag taagagaacg ccagaaattc caagcgttaa gcaaagaaat
     6841 cggccgttcc tataatgaca taatggacga gctttatagc aaaaaccgcc agcgattcct
     6901 ttacttatcc cgtcatcaat ctgaagcgat cagcgaatat gcaaaagaac tagatattga
     6961 tgcattagtg attaaagtcg aatcacgccg tttttacccg ctggcagaag aatcttcaca
     7021 gttgatcggt tttactgata aagatgatat gcacggttcg gaagggctgg agcgtagttt
     7081 tgattcctta cttatcggca aaaacggtaa acaaattatc cgaaaagatg ccaaaggcaa
     7141 tattgtggaa aatatccgtt cggagaaaca gtacgatccg caagatgtta tgttaagcat
     7201 tgatgaagaa ttgcagtcta tggtgtacgg tgaaatcaaa aaagcggtac aggaaaataa
     7261 tgcggaatca ggcactgcgg ttttggttga tgtgcaaacc ggcgaaattt tggcaatggc
     7321 aaatgcccct tcattcaacc caaacaaacg ggatagtttt aaacctgaat taatgcgtaa
     7381 ccgtgctatt accgacactt ttgaaccggg ttcaacggtg aaacctttta ccgtattaac
     7441 cgcattacaa aatggggtaa cttatcgtga tgaagttatt ggcactcgcc catttgtcgt
     7501 caatggacat acaattaaag acgttgctcc aagggacagc cttacgctta ccggcatctt
     7561 gcaaaaatcc agtaacatcg gggtcagtaa attagccctc agaatgccgg caaatgcgtt
     7621 agtggatact tacacgaaag tcggtttcgg taaagacacc ggtttaggct tgggtgaaca
     7681 acgaggtacg aacggtgatc gcaaacgttg ggcggatatt gaacgtgcta ccctttccta
     7741 tggatacggt ttaaatgtga ccccattaca acttgcccgt gcttatgcga ctttaggaag
     7801 tttcgggctg tatcgcccac tttcgattac caaggtcgat ccgccggtga ttggcgaacg
     7861 ggtgctaccg gaaaaaacca cccgagatgt cgtgcatatg atggaaagcg ttgcggctaa
     7921 aggcgaaggc ggacaccgtg cagcggtgga cggctaccgt gtagccatca aaaccggtac
     7981 agcacgcaaa ttagaaaaag gtaaatatgt tgaaaaatat attgcttata ccgcagggct
     8041 tgccccggca agcgatcctc gctttgcatt agtggtatta atcaacgaac ctaaagcagg
     8101 caactattat ggtggctttg tttctgcacc actgttctca cgcattatgg gttacacctt
     8161 aaaacaacgt aatattaagc cggataactt aacggaaaat acccaaagtg ccgtgcgtga
     8221 aattaaatta gaacgcccta tcaattaagg ttagctatga aacgtttact cccttttctg
     8281 acagaacttg atgcttgggt ggaagaactc accgagctaa aacaaatgac acttgatagt
     8341 cgccaagttc aagccggtga tctctttgtt gctttaaaag gacatcaagt cgatggcaga
     8401 aaatttattc cgaatgcgat agaacaagga gcagcaatta ttttggctga ggctgatgaa
     8461 gatcaaccgg aaattgagct tgatccgaaa tttgcaaaat ttaacctaga tcgcaccgct
     8521 tgttgcaagg ttgtctctgt tccacgtctg ccaaaattgc tctctgaaat tgcaggagct
     8581 ctttaccata acccgtcaca caagctgact ttagccggca ttaccggcac gaacggtaaa
     8641 acgactactg ctcaattatt agcacaatgg cataatttat tgggtgggaa atcggcagta
     8701 atgggtacta tcggcaacgg tttgtacgga caagtgcaag aagcggtaaa taccaccggc
     8761 tctgccattg aaattcagca taatcttgcg aactttgttg aacttggagc ggatttttgt
     8821 gcgatggaag tcagctctca cggtttggct caatttagag ccgaagcctt ggattttgat
     8881 ttagcgatct ttacgaattt aagccgagat caccttgatt accacaacac gatggaagag
     8941 tatgcccaag ccaaattccg tctcttcaac gaattaagca ccaaagctca agtaattaac
     9001 gccgatgatg aaatcgggcg tgaatggctg acacaattac caaatgcagt tgcggtaagc
     9061 accgatccga agtttgcagg caaccaccaa tttgtcaaag ccactgcggt aaaatttacc
     9121 ctacaagggg caagtattgc gtttgaatca agttggggca atggcgagtt acatagccgt
     9181 ttaatcgggg ctttcaatgt aaataatctg cttaccgcct ttgccggact tttagctctt
     9241 ggctttgata ttcaagaact ggttaaaacc gccccgaatt tagttggcgt agcaggtcga
     9301 atggaatgca ttaccgctcc aaataagcca atggtaattg tggactatgc tcacacccct
     9361 gatgctcttg aaaaagccct acaagcggct cgtttacatt gcgaaggcga actttggtgc
     9421 attttcggct gtggtggaga ccgtgacgcc ggcaaacgcc cattaatggc gacgattgcc
     9481 gagaaattaa ccgataaagt gattgctaca gacgacaacc ctcgcaccga agataatcaa
     9541 aagattatgg cagatattgt gaaaggtttt agtaaacccc aacaaattat ccacaatcgt
     9601 gaagaggcaa ttaaaaccgc cattgagcaa gctaaagcca cagatgtgat tctaatcgca
     9661 ggcaaaggtc acgaagatta tcagattatt ggcacagaga agttacattt ttcggatcag
     9721 gaaacggcaa ggaaatatct gttttaatgt cactttcttt gcacaagcaa agaaaagtga
     9781 cagtgacaaa tacaaaatgc tcgtaattaa ttttataaag aaaaagataa atcaatgata
     9841 aaactaacca cccaacaaat cgctgaaatc ctaaatgcca aattgatcgg caatggcgaa
     9901 actgtggtag aaaccaccag caccgacacc cgacaagcgg ttgaaaacgg cttatttttt
     9961 gcattaaagg gcgagaactt tgatgctcat cactatcttc aaaatgcagt cgcacaaggt
    10021 tgtgtagcgg ttgtggttga gcgtgagtgt gaaattgatg taccacaact tgtggtggca
    10081 gatacccgtt tggcattggg ggaattggcg aaatggttaa aagctaagct gaacccgaaa
    10141 acggtagcga tgacaggttc ttccggcaaa acaaccgtca aagagatgac ggcaaaaatt
    10201 ctgcaaaaaa tgaccgcttg cgaacaggaa gtgctttata cttttggtaa cttaaacaat
    10261 gacttaggtg taccgatgac cttgcttcgc ttaaccgaac agcataaata tgcggtcatt
    10321 gagctggggg caaatcatat cggtgaaatc gcttatacca ccgctatcgc tcagcctgat
    10381 gcctgcttgg tgaataatgt ggctgcggcg catttagagg ggtttggttc attagcaggc
    10441 gtggcacaag ccaagggcga aatttatcgt ggcttgaaag aaaatggcaa agcgattgtc
    10501 aatttagctt tttattatcc gcaatggcaa aaagagattg gctcacggga aatgcaatct
    10561 ttctcttata tcgggtcaga ttcggattct tacgcagatt actgggcgga agatgtagaa
    10621 ctgcatttaa gcggttcacg ctttacgcta cataccccac agggcgaaat tgctattgat
    10681 ctgccttatt tgggcaaaca taacgtgagc aatgccttag cggcaacttc gctggcgatg
    10741 tctgttgggg caacgctaga agcagttaag gcagggcttg agcagcgttc aatggtaaaa
    10801 gggcgtttgt atccggtgca aattaacgac cactgtttgt ttattgatga cacttacaac
    10861 gccaatgtgg attcaatgaa atcggcgatt tctgttttgg aaaattatcc ggcttatcgt
    10921 atttttgcag tgggtgatat ggcggaattg ggtgaagaaa gtagcaaatg ccaccaagaa
    10981 gtagcggatt ttgccaaaaa tgcagcatta gatttaatcg tgagctttgg aaaagaaagt
    11041 gcggtaatca gccaacaggc agaacatcat tttaccgata aacagcaaat ggttgatttc
    11101 ctactgccga ttattttgca aaaaatcgaa caaaaacaac cgcttgtatt attggcaaaa
    11161 ggctctcgta gccagaaaat ggaaaccgtg atttccgaat tgtgtaaacc gtttaaccta
    11221 aatttctaat tatttttaag gactaagcaa tgttagtttg gcttgcagaa ctattggttc
    11281 aatataacac agcgtttaac gttgtctctt atattacttt ccgtgccatt atggcgttat
    11341 taactgcaat gggaatcggg ctttggatcg ggccgaaagt aattcgccgt ttgcagatct
    11401 taaaatttgg tcaggaagtg cgtaatgacg gaccggaaag ccatttcaaa aaacgtggta
    11461 cgccaacaat gggcggagtg atgattctcg cagcgattgg ggtgagtaca ttgctttggg
    11521 cagatctgcg taattcctat gtttggttca cgctgtttgt gctatttggc tacggtgcag
    11581 tgggctttgt tgatgattat cgtaagatct ctcgtaaaaa taccgatggt ttgattgccc
    11641 gttggaaata tttttggtta tctgctatcg cattagtggc ggtgtttggt atgtatgcgg
    11701 tgggtaaaga taccgctgca acacaattag tcgtgccgtt ttttaaagat gtgatgccgc
    11761 aactcggttt attttatatt gtgttatcct atttcgtgat tgtcggcacc agtaatgcgg
    11821 taaacctaac ggacggctta gacgggcttg cgattgtgcc aactattatg gtcgcatctg
    11881 cctttgcgtt aattgcgtgg gcaaccggta actttaattt tgcacaatat ttacatattc
    11941 cttttattcc aaatgccggt gaattggtta ttttatgtac tgcgattgtg ggagcaggtt
    12001 tagggttctt atggtataac acctaccctg cacaggtgtt tatgggcgat gttggctcac
    12061 tttcgctcgg cggtgcatta ggcgtgattg cagtattagt tcgccaagaa ttattattgg
    12121 tggtaatggg cggtgtattt gtggttgaag ccctctcggt gattttacaa gtaggttcat
    12181 acaaattacg ccaaaaacgt attttccgaa tggctccaat tcaccaccat tttgaactca
    12241 aaggctggtc tgaaccacgg gttatcgtgc gtttctggat tatcacgcta atgctggttt
    12301 tagtcggctt agtaacattg aaattacgtt aattgattga ataaggtgca ttgagataat
    12361 gtaccttatg ttatttttaa attacagaat aaattatgca aaaacaatac gaaaacaaaa
    12421 ccgttactat tatcggctta ggtacaacag ggttatcttg tgtggatttc tttaagaaaa
    12481 aaaatgccaa tatccaagtg attgacaccc gagcaaaccc agccggagcg gataaattgg
    12541 acgtaactat tccgttgcat accggtgggt taaacagtga atggttgctg aaatctgatc
    12601 ttattgtaat tagcccaggt ttagccgttg ccacacctga aattcagcaa gcgattcaag
    12661 ctggtgttga aatagtcggt gatgttgaac tgttttgtcg ggaagcgaaa gctccgatta
    12721 ttgcgatcac cggttcaaac ggcaaaagta ccgtaacgac tttaactacc gaaatggcga
    12781 agcaagcggg gattaaagtg ggaatgggtg gcaacatcgg tgtgccggta ttaagtttgc
    12841 tggagggcga ttatgacctt tttgtgctgg agctatccag cttccagctt gagacaactt
    12901 attcactgaa agcaaaagct gccactgttt taaatattag cgaagatcat atggatcgct
    12961 acgattcgat tgccgactat cgccaagcga aattgcggat ttacgataat gcggaaaata
    13021 tcatcgtaaa cggtgaagat gcccaaactt acccgataca agcggtcaaa aaagtgattc
    13081 gttttgcaga acaaaatgcg gaatatagcc tacgcaatca gatgctttat tcaggcgatg
    13141 atgcaattat ccaaactaaa cagatgctga ttagcggccg tcataatgag atgaatgcgt
    13201 tagcggcgat ggcattagca gaagcggcga atgttcctcg tgaaggcatt gtgaaagctt
    13261 tgcaaattta tggtggtttg ccacaccgtt tccaagcggt ggcaaccaat gatggcgtgc
    13321 gttgggtcaa tgattccaaa gcgaccaatg tgggtagcac ggtagcggca ttaaacggct
    13381 tgaatattgc cggtacactt tatttacttt taggtggtga cggcaaggct gcggattttt
    13441 ccgaactcaa accattaatt aataagccaa atattgtctg ctattgtttc gggcaagatg
    13501 gagcggcatt ggcagaatta agcgaacaaa gtgtgttggt tgaaacgatg cagcaagcaa
    13561 ttgaagcaat ccgcccacaa ctgaaaaaag gcgatatggt actgctttct ccggcttgtg
    13621 ccagtttaga tcagtttaaa aactttgagc aacgtggcga ggtatttatg gcattagcac
    13681 aaggacagca cgcataatgt tagaaagaat taagtcggaa tgggataaat gggtcagatt
    13741 aaccccttct aattcacttt atgacagaac tctaatttgg ctatttttag gcctgctaat
    13801 tatcggtttt gtgatggtaa catctgcttc actcccagtt agcacaaggt tgaataacga
    13861 tccattttat tttgcgattc gagacgggct gtatattatt gcttcaatta tcttttgtta
    13921 tgtttttgta caaatcccaa ttgaaaaatg ggaaaagcat aacttggcac tgttttttat
    13981 ttcaatcggt tttttaatcg ccgtactcat tttcggccgc tcaattaatg gtgcagtgcg
    14041 ttggattccg ttgggtattc tcaactttca gccggcagaa ttagccaaat tagcggtcat
    14101 ttgctatttt gccagtttct atgtacgtaa atatgatgaa attcgtaaag aaaaagccag
    14161 tttctggcga ccggcagtga ttttattctt attcggcttc ttgctgattt tgcagccgga
    14221 tttaggtagt acctttgtat tatttgttct cactttctca atgcttttca ttgtgggggc
    14281 aaaaattatg caatttatgt ttttaggcgt ggtgggaacg gtgttatttg cggtgttgat
    14341 tttaacctcg gaataccgtt taaaacgtgt tacctccttt atggatccgt ttgctgatgc
    14401 ttatggcgac ggtttccagc tctcgaactc acaaatggca tttggacaag gtgaattttg
    14461 gggacaaggt ttaggtaatt cggtacaaaa gctagaatat ttgccagagg cacatactga
    14521 ctttgtaatg gcggttattg gtgaagaatt tggtttcttt ggtattgctt gcatcgtact
    14581 tttacttatt ctattaacgg tgagagcctt aaaaatcagt aaagaatctt tggtgctgga
    14641 agagcgtttt aaaggctata tggcatttgg gattgcgatt tgggttttcc tacaaggctt
    14701 cgtaaactta ggggtggcat caggtttgct gccaacgaag ggcttaacct tcccattagt
    14761 gagttatggt ggttcgagtt tggtaattat gtcgattgcg attgcagttt tgctacgaat
    14821 tgaccacgaa aaccgtgccg agcgaatcgg gcacgcaaaa attaaaacca tctagccgag
    14881 cggtctgcaa cgttacaatt tgcaaaattt tacaaaaatg agaccgcttg tattagctgg
    14941 aaagcataaa aattcagtaa aatagcacgt tttagaataa aaaagagttg aaaatggcga
    15001 agaaattatt agtaatggca ggtggaacag gggggcacgt tttccctgca attgccgtag
    15061 ctcgagagct acaaaaacaa ggctggcaaa tccgctggct ggggacgaaa gaccgaatgg
    15121 aagccactct tgtgccgaaa cacggtattg agattgattt tattgaaatc tcaggcttgc
    15181 ggggtaaagg cgttgcagca ttgttgaaag caccgtttgc tatttttaaa gcggtgatgc
    15241 aagcccgcaa aatcattaaa aattatcagc ctgatgcggt gttgggaatg ggcggttatg
    15301 tgtccggtcc gggcggtatt gcggctaaat tatgtggcgt gccggtgatt ttacacgagc
    15361 aaaacgcagt tgccggccta accaatgtgt ggctatccaa aattgcccgc aaggtgttac
    15421 aggctttccc tactgctttt aaagaggccg aagtagtagg taatcctgta cgtgaagatt
    15481 tatctgctct tccgctgccg aatgaacgtt tcgccaaacg aggctacccg attaacattt
    15541 tagtaatggg tggcagccaa ggggcaagag tgatcaacca aaccgtgccg gaagtggcaa
    15601 aagtgttggg taataatgtg tttatcagcc accaagtcgg taaaggtaat ttagccggta
    15661 tcgaagaagt ctatcaacaa accggcaatg ggattgcttc cgagtttatt gatgatatga
    15721 aagccgcgta tgagtgggca gatttggtga tttgtcgttc cggtgcgtta acggtgtgtg
    15781 aaattgccgc cgtaggtctg cccgctgtgt ttgtgccgtt ccaacataaa gacaggcaac
    15841 aatttttaaa cgctaactat ttggcggcag atggagcagc agtgattatc gaacagagcg
    15901 atttcactcc aaaggcatta cagcaagcct tagaaccctt aattgccaat cgccaactct
    15961 tattggatat gtcaatgcga gccagagcaa aatccacccc aatggcagca aaacgtgtag
    16021 cggaagtgat tattcaagaa gcgaattaac ttactacaag cggtcgtttt tggtaaaaat
    16081 tttgcaaaat gtaaccgctt gcgaatagat taaaaggaca ataaatgaaa aatttccaag
    16141 ataaagtcaa aaaattagtg cctgaaatgc gtcgtgtcag ccaaattcat tttatcggga
    16201 ttggcggagc cggtatgagc ggcattgcgg aagtgttatt aaacgaggga taccaaattt
    16261 caggttcgga tattgcagac agagcggtta cacaacgtct atccgcagcc ggagcgaaag
    16321 tctttatcgg acaccaagca gaaaatattg ctggggcgag tgtggtggtg gtttctactg
    16381 cgattgatga aaccaaccct gaaattattg ccgctcgaga agctcgaatt ccggtgattc
    16441 gccgagcaga aatgcttgcc gaaattatgc gttttcgcca cggcattgcg attgcaggca
    16501 cacacggtaa aaccactaca accgcaatga tttcaatgat ctacaccgaa gcaaaattag
    16561 atccgacttt cgtgaatggc ggtttggtaa aatctaccgg caaaaatgct catttggggg
    16621 cgagccgtta tttaattgcc gaagccgatg agagtgatgc ttctttcttg cacttgcagc
    16681 caatggtgtc ggtagtaacc aacattgagc ctgaccatat ggacacctat ggtggcgatt
    16741 ttgagaaaat gaaagagact tacgtacgat ttttgcgtaa cttacctttc tacggcttag
    16801 cggtaatgtg tgccgatgat gaaacggtaa tggaaattgc tcctcgtgtc ggcagacaag
    16861 tgattaccta tggctttagc gaaaatgcgg attaccgaat tgaagattac cagcaaaccg
    16921 gcttccaaga gcattacacc gtagtttgcc caagcggtga acgaattgat gtgctgttga
    16981 atgtgccggg tatgcacaat gccttaaatg caaccgctgc gttagcggtg gcaaaagaag
    17041 aagggattgg caatgaggcg attttagctg ctttagctga tttccaaggg gcaggacgcc
    17101 gctttgacca attaggttca tttattcgcc cgaacggcaa aattatgtta gttgatgact
    17161 acggccacca cccaactgaa gttgatgtaa ccattcaagc cgctcgttca ggctgggaag
    17221 gtaaacgtgt tgtgatgatt ttccaaccgc accgctattc tcgtacccgt gatttattcg
    17281 atgaatttgt acaagtactg tctaaagtcg atgccttgat tttattggat gtgtatgctg
    17341 ccggcgaagc tccgattgtg ggggcagaca gtaaagcctt agcccgttca atccgtaatt
    17401 taggtaaggt cgatccgatt ttggtttcag ataccgatca acttggcgag gtattagatc
    17461 aaattattca agacggtgat ttaattttgg cacaaggtgc gggcagtgta agccgcattt
    17521 ctcgtggctt ggctgagagc tggaaaaatt aaatactctc tccccttgcg ggagagagac
    17581 cgaaaaatac ttcgttcaga agtatttttc agagagaggg tatatattta aaggatttca
    17641 aatgagtatt aaaaacgaaa aaattgccgt cttattcggt ggcgtttcgc aagagcgtga
    17701 agtttcactg aattctggtg cagcggtaac agctgcatta aaaagtttag gctataacgt
    17761 agaaggcatt gatactaaag aattcccgat tgaaaaatta aaagaaaaag ggattcagcg
    17821 tgtatttaat attttacacg gcggtattgg cgaaaatggc gtactgcaag gtgcgttaga
    17881 gcaaatgggt attccttaca ccggttgtgg cgtaatggca tcagcagtga cgctggataa
    17941 attccggact aaattattat ggaatgcagt aggtttacca acagcggata tggtggtggt
    18001 tcagcgtgga caagcggtcg attttgacca aattattgca aaattaggct taccggtatt
    18061 tgtgaagtct tcgtgtgaag gctcaagtgt gggtgtattc aaagtaaaaa ccaaagaaga
    18121 attggcaccg gcaattcacg aggcattaaa gtttgattcc attgtgttgg tggaagaatt
    18181 tttagccggt aatgaatatt ctgtaccggt attagacgga caagttctac cggcggtaca
    18241 agtaattccg gacggcgaat tttatgacta ccacgcaaaa tatgtgtcgg ataacaccca
    18301 atatgtagta ccggcattaa atgacgaacg ccaagcagag gtcgccaaat tagtgaaagc
    18361 ggcttacgat gtggttggct gtcgaggttg gagccgtatt gatgtgatgg aagatggcga
    18421 aggaaaattc cgcttggttg aagtaaatac caacccggga atgaccagtc acagtatttt
    18481 cccaaaatca gcagcaacag ttggtatttc ctttgaaaaa ttagttgaac gcgtattgga
    18541 gttaagtgct taatgggcat cttccgcaag aagaacagca caaatgtgcg tttgaatgtt
    18601 cttaatccaa aaacacctgt ttctccacgt aaatggctag tattcattaa gccattaatt
    18661 gtgttattat gtacactatt tgtattcggt gtgtatagta attggcaaaa tatactcgaa
    18721 agttgggata aaacaccgat tcgcgcttat gcattaacac ataaaacaca atttacaaca
    18781 aatgcagata ttcgtgaaac tttgtctaaa gaacctgctc taaaaggata tttcggacaa
    18841 aatattcaag aggtcaaaga taagctttta tctatacctt gggttaaaga tgtggctgtt
    18901 cgtaaacttt atcccgaccg attaagtatt actttgctag aacataaacc cgttgcaatt
    18961 tggaatgata ctaactttgt ctcagaacaa ggaaccgtct ttagtttacc gaaagacaga
    19021 atcgataaaa atggacttcc attactctat ggtccagata cggaaggaaa aagtgtatta
    19081 gaggcatgga ataaaatccg agctgatcta aatgcccgca acttggattt aacctccgtt
    19141 tctgtagata atcgaggttc ttggacaatt aggctttcta atcaagtaga attaagatta
    19201 ggaagagggg aatggacttc taaaattgat cgttttgtaa caatctttcc tgaaattcat
    19261 atacctgaag gtaatagatt agcttatgtt gatttacgtt atccacatgg cgcggcggtt
    19321 ggatttagtc ctataaaaaa ttaaactcag tgaacacaaa aacattatga caaaagcagt
    19381 agaatcaaaa attatcgttg gtcttgacat tgggacatca aaggttgttg ccgttgttgg
    19441 tgaagtatta cctgatggtg taattaatgt tatgggagct ggcgtttgcc cttctaaagg
    19501 agtcgatcga ggtggcgtta ttgatcttga cgctgccgtt aattctattc aaagagccat
    19561 tgagcaggcg gaatctattg ccgattgtca aattatgggt gtaacactgg caatctcagg
    19621 tcagcatatt gttggtttaa atgaagcagg atcatcaaca ttatctggag gtattgttac
    19681 ccaagataat attgaaagtg ctgtggatat ggcaaaggca attaaattac aagatggctt
    19741 agaaacatta cacatgattc cacaagaata tcacgtggat agattgcctg cgactaaaaa
    19801 tccaattggc ttatcgggta tgcgtttaca agttcaagca cacttaattg cttgccatca
    19861 agcttggtta aaaaacttaa aaaatgcggt tgaacatgct aaattaaaag tcgatcaagt
    19921 tgtattttca ggtcttgcct ctagctattc tgttttaact gaagatgaaa aagagctggg
    19981 tgtttgctta attgatattg gtggcggtac gatggatgta ctagtttata ccgatggagc
    20041 attaagatac agtaaagtca ttccatttgc aggtaataac attactgatt atttggctcg
    20101 tgtatttacg acatctcgcc ctgaagctga aagccttaaa gtaggttatg gtagcgcaat
    20161 ttcgcctcca acccataatt cagataaaaa aattgaggtg gcaggactag gtggacggat
    20221 ggcaagaacc tttacccgag ctcaggtggc aacggttacc tcacaatgtt ataacgattt
    20281 gctaaaagtt gttgaagagg aattaaccca attacgccat gaacttttcc aaaaaggaat
    20341 aaaacaagag ttaattgccg gtgtagtcat tactggtggt ggctcacaaa ttgaagatat
    20401 tgtagaatgt gctaaatcaa tttttggttc acaagttcgt gttggatatc cactcaatat
    20461 tactggctta accgattatg tgaataaacc acaatatgca acagcattag ggcttttaca
    20521 atatagccac tataattata ctgaacaagg tacaagtaca gattctaatg aagatatctt
    20581 tgctcctatt ggtcgtggaa ccaagaaaat cattgacttc ttcaaatcaa aattttaata
    20641 gtgttaaaat aaacacgcag ggagataaaa atgtttgaac ctattattga acaaacgcaa
    20701 gatgctatca ttaaggtagt gggtgttggt ggcggtggcg gtaatgccgt agacagaatg
    20761 tctcgctctg ccgatgatat taaaggtgtt gaattctttg atgtaaatac tgatgctcaa
    20821 gttttaagaa aaagaaccac acgccaaact atccaaattg gagcaagtac aactaaaggt
    20881 ttaggtgcag gtgctgatcc aatggtagga aaacaagctg ccgaagaaga tcgtgaggct
    20941 atcgctaatg cattaaaagg tgcaaatatg acctttattg ctgttggtat gggtggaggt
    21001 accggtaccg gagcagcccc tgttgttgct caaattgcca aagagcaagg ctcactaact
    21061 gtaggcgttg taacaaaacc tttccgcttc gaagggcctc gccgtatgcg ctttgcagac
    21121 caaggcatta aagagctttc tcaatatgtt gattctctta ttataattcc taatgataaa
    21181 ttacgtggtt taggtaagca aacaacagca gtagatgcct ttgccgcagc aaatgatgtt
    21241 ttaagtaact gtgtgcttgg tattaccaat atgattacca gttcgggcgg ttctaccggt
    21301 gcagatatta acgtggactt cgcggatgtt cgtacagtaa tgtctggtaa gggacacgct
    21361 atgattggta caggttttgc tgaaggtgaa gttggtgaag gacgagcgga aaaagcaatg
    21421 aatgatgcca tttctagtcc attacttgaa aatgtggata tttcaggtgc aagtggtatg
    21481 ctcatcaaca ttagtgctgg gacagatttc ttattagaag aagtttatgc tatgatggac
    21541 ttaatttatg gctttgcaac agaagatgcc gcaattgtat ttggttgtaa ctattaccct
    21601 gaaatggatg gaaaagtgag tgtcactctt gtcgcaacag gaataggcca acctgaggaa
    21661 gctctacata tgccacataa ggctcagcca gtatatgcac agcaaggaaa tcatgttcaa
    21721 gctacacagc ctactcagcc aaaccatgcg gtacaacaac caaactttac tcaaccgtca
    21781 agttatggtc agccaaatca atttagtggt caaccacaac cactacaaca acctgtggct
    21841 ccaaaaccac aatcggttga tacagcacaa atttttaacc caaattcaat tccgggtttt
    21901 atgagaaata gtcaataact tccactcatt tcggaagtaa ggacaaaata tgactaaaca
    21961 aagaacatta aaacaagcag caaaagtaac aggtattggc ttacatagcg gcaaaaaagt
    22021 aaccttaacg ttacgccctg ccccagctaa tacaggagtt atttatgccc gtaccgattt
    22081 agagcctgtc gtttatttcc ctgcaagcgc agactctatt cgtgatacac aattatgcac
    22141 atgtatggta aatgatgatg gtgttcgtat ttcaacagta gaacatttaa acgcagcaat
    22201 gtcagcatta ggcttagata atgttattgt tgaagtagat gcacctgaaa ttccgattat
    22261 ggacggtagt tcaagcccat ttatctatct tttattagat gccggcatcg aagaacaaga
    22321 tgcaccgaag aaatttattc gcattaaaga aacagtacgc gttgaagaag gcgataaatg
    22381 ggccgaatta aaaccataca ataaaggttt taaattgaac ttcactattg actttgagca
    22441 cccattaatc cccaaaaatg tagggagcta ttccattgat ttttctgctg agaactttat
    22501 tcagcaacta agtcgagcga gaacctttac atttatgaag gatgtagaat acttgcaatc
    22561 tattggtctt gcattaggtg gaagccttga taatgctatt gtattagatg attaccgaat
    22621 tctaaatgaa gacggtttac gatttaaaga tgaactggtt cgccataaaa tgctggattc
    22681 gataggtgac ttatatatgt gtggttataa tatcctaggt gaatttagtg catataaatc
    22741 aggtcatggc gttaataata aattattaag agctgtattg gaaaataaaa acgcttggga
    22801 gtttgttacc tttgaagata aagcagaagt cccacaaggt taccaagtac aacaagaaat
    22861 ttttatttag tctaaatggc ttaaaagacg aacagaaatg ttcgtctttt attttgcaaa
    22921 ttttttctaa tatttaaccg cttgcaaccc gcctcattag cgattttttc gcccttatct
    22981 atttactgtt taaaaaaaca gttgtactat atacccatac agttaatctt gtgtggtgta
    23041 ttatgcttac tcaacttact attaataatt ttgcgattgt tcgccatctt attcttgaat
    23101 taaatgaagg tatgtctgtt attaccggtg aaaccggtgc cgggaaatca atcgctattg
    23161 atgccttagg gctatgcttg ggttatcgtt ctgaaagtgg gatgattaga aacggttcgg
    23221 acaaagcaga tataagtgca acctttacta tgcagccaca tagccctgct tatttatggc
    23281 tacaagagca tgaactgctt gatgaagata atcctcaaga gtgtatctta cgccgtatga
    23341 ttaatattga tggacgctca aaagcctttg taaataaccg atcattacct gtttcacaac
    23401 ttcgagagtt ggggcaatat cttattcatt taaatgggca acacgcacca caattactcc
    23461 taaaaaacga atatcaactt gaaatagtcg atcattatgc cacgttacag cctctattga
    23521 tgaagatggc aaagcaatat gggaattgga aagaactgaa taagcaggta aaaaatttcc
    23581 aaaaacagtg ccaagaaaat gaagctcgca aacagctatt agaatatcaa gttgaggaat
    23641 tagatgaatt tgcaattaaa gcaaacgaat ttgaagaaat ggaagagaaa tacaatatct
    23701 tgtcttcttc cgaggagctt actgagcttt cagatgaaat cacacgctta ttaagtgatg
    23761 atgaaatcaa tgcaaacagt ctgatttata aagcgttacg caatgcagaa aacttggctg
    23821 aggttgatag ccaatatcag ggcattttag atatgcttaa tgaggccctg attcagattc
    23881 aagaagcaag ctccgaagtg gctcaattag ccagtaaaat tgaacaagat ccggaattac
    23941 tacaggagct ggataataga attgcacaaa ctatatcgtt agcacgcaaa caccatattc
    24001 aaccgaatga attatggcaa tatcacgcga cattaaaagc agaactacag gaattactgg
    24061 attttgaaga taatgaagaa caactcattc aaaatgaaaa acaagcttac caagagtgta
    24121 taacattagc agaagaaatt taccaaaaac gtctagatgc cacacataag ctctctgaac
    24181 aagtaacaaa acaaattaaa cacttagcaa tggaaaacgg tgagttcttt attcatatag
    24241 aacataatct tgaaaaaatg tctgcaaatg gtgctgacta tgtcgagttt aatttacgca
    24301 gcaatttagg acaacaaccc caaccactgg caaaaattgc ttctggtggt gagctatctc
    24361 gcatttctct ggcagtacaa gtgcttaccg caaataaact ttctacgcca actattattt
    24421 ttgatgaagt tgatgttggg attagtggct ctgctgccac tacagtcgga aagctactgc
    24481 gtaagctagg cgaaaaatgc caagtattat gtgtaaccca tttacctcaa gtggcaagtt
    24541 acggcaacca gcagtttaat gtacaaaaat atgtagaaaa taatcaaact gaaacgcaaa
    24601 tgacattact cacagaagca gagcgtattc acgccttggc tcgactatta ggcggaagcg
    24661 aaatcaccga aacggtttta gctaatgcaa aagaaatgct aaatttagcg aaaaataacg
    24721 aatagccaaa aagaaaaatc gcatcaatga atattgatgc gattatgaat ttggtggagg
    24781 taaacgggat cgaaccgatg acctcttgca tgccatgcaa gcgctctccc aactgagcta
    24841 taccccctta atacaagcgg aataatatgc tttccgcttg aagatgtcaa tggctttaat
    24901 caagcaaggt gttgattgat taaaaactag ctattcgcct gaataaactc aatcgctttt
    24961 tgtaagcgag ctaaggttct ttctttaccg actaatttgg cggtgatatc cattgacggc
    25021 gattgcccac ttcctgttac cgctaaacgg aacggcatac cgactttgcc cataccaatt
    25081 tccagctcgg cagcggtttg gttcattgcc tcgtggatat tttccaccgt ccagtcattg
    25141 caagcggtca atttttccaa taattttgca agtggagcaa cggcttcggc tttgaagttt
    25201 ttagcagcag ctttttcgtc gtaagcctta aattcttgat agaaatagcg gctgctttcg
    25261 gcaagctctt ttaaggtttt gcaacgttcg cccagtacag ggataatttc ttccaatttt
    25321 ggaccgcttg tgaaatcaat gccttgagtt tgcatatgcc attcaagatg ttttgccaca
    25381 taacttgggg caagactgcg gatatagtgt tggttcaacc attgtaattt ttcagtgtta
    25441 aatgcacttg ccgatttact gactgaatga atgtcgaaca gctcgatcat ttcgtcacga
    25501 gagaaaatct cttggtcgcc gtgtccccag cctaaacgca caaggtagtt aattaaggct
    25561 tccggtagat agccgtcatc acgatattgc attacgctca ccgcaccgtg gcgtttggag
    25621 agtttttgcc cgtcatcgcc gttgatcatt gaaacgtggg cgtaggttgg gattggtgct
    25681 cctaatgctt tcaaaatgtt gatttggcgt ggtgtattgt tgatatggtc ttcaccccgc
    25741 acaacgtgag taatgcccat atcccagtcg tccaccacca cacagaaatt gtaggtcggc
    25801 gagccgtcag tacggcgaat aatcaagtca tctaattcgc tattggcgat ttcaattcgc
    25861 ccacgcaccg catcgtcaaa tactaccgag ccttcggtcg ggtttttgaa acgcacaaca
    25921 tgtggttggc tcgggtcgtt ttgcggattg tgtaagcagt gctgatcgta tttcgctttc
    25981 tctttatttg cttcttgctc tgcacgtact tcgtctaaac gttctttcga gcagtagcaa
    26041 cggtatgccg tgccggcttc taacatttgg tcgatcactt ggttatagcg atcgaaacgc
    26101 ttggtttgaa agtatgggcc gtgttcccac gccaaatcca gccattccat accctctaaa
    26161 atagcggcgg ttgcctcagg ggttgagcgt tcaagatcgg tatcttcaat acgtaacaca
    26221 aattcaccat cattatgctt agcatataac caagagtaga gcgcggtcct cgccccacct
    26281 acgtgcaagt agccagttgg gctaggtgca aaacgggtac ggacttttac attcggatct
    26341 aaggggaaaa gggtttcaat tttcatttta tattgcctaa ttgatgattc ttaagaaaaa
    26401 cttgtctatt ttactacgct tgagggtgaa tcatagtaaa aaattaaatt tgcggggtaa
    26461 aaaatcagca aacggggtaa aaagaacaaa aagggtttga cgaaacagca aaaatctcta
    26521 taattcactt ccacaacagc gggcgattag ctcagttggg agagcacctc ccttacaagg
    26581 agggggtcac tggttcgaga ccggtatcgc ccaccacttc aacttaagtg tttcgttgtt
    26641 gtatcacaat ctaattttga aaacgggcga ttagctcagt tgggagagca cctcccttac
    26701 aaggaggggg tcactggttc gagaccggta tcgcccacca cttcaactta agtgtttcgt
    26761 tttcaaattc cctttgattt taagtctcaa gtgggcgatt agctcagttg ggagagcacc
    26821 tcccttacaa ggagggggtc actggttcga gaccggtatc gcccaccact taagactaaa
    26881 atcataaact tcaaatggcg atttttcgta tctgaagggc gattagctca gttgggagag
    26941 cacctccctt acaaggaggg ggtcactggt tcgagaccgg tatcgcccac catctattta
    27001 gtatcaaagc ctaaaactta tatcataatc taataacccc tttaaagtca taactaaaaa
    27061 gggaaaataa attcagatgg cctgttgcat ctgcataaat gcctttctac gtgcagaaat
    27121 taatcttttt ctatctttaa tttgcttaat ttgacgcagg cgtaatttcc ttgtcagtag
    27181 ggatttcttt ttcttcaatt ttctcataag aagaattctc ctgttttatt tgaccatctg
    27241 gtaaattttc ttgttcttgc atcttttgct cttcaactcg ttgataatta ataatactac
    27301 cataataacg ccgtatattt tcaacatact gtaatgcttc atatcctcga gcatagccgt
    27361 attttagatt agcataataa cgtttttccg caagcaatgg cagattcttt ttaacatcaa
    27421 gccaattatc cggatcacca ccaagttgtt ttgttaatcg acgaagatcc aacaagtgcc
    27481 ccaatcccat attatacgct gctaggctat accaaattct atctgcttga ggaatagttt
    27541 ccggcatttg tctcattagc atatgtaaat attcagatcc cgctttgata ctttgtgcag
    27601 ggttagtcct atcagaaatt ttcattctat ctgccgtatc ttttgtcagc atcataattc
    27661 ccctcactcc ggttggagaa gttgcattag gatcccaatg agactcctga taggcgatag
    27721 ccgccagcat ttgccattct aattcacctt tatgtgcccg aaaaaggggt tcatatttag
    27781 gtaaaatagt tttaattgca ttcaaatagc tctgaatatc aacataatca aacttagcta
    27841 aatgattaaa atatttctct tctattcttg atatcagccc agtttccata gaataagaca
    27901 taaaatccag cagcacagat tgcagttcac tataagaact atttgctaaa taccataaca
    27961 ctgggttttc atcagttaaa tcaaacccta cggctaaatt aggattaata tgctgtgcag
    28021 cagaaatatc aaccgacatt gcaattgtat gtgaaatttc accttgtgcg acttttaaca
    28081 atagctcttc ttgtgtaaat ttatccgaga tctgccattt tagttccgga ttatccacct
    28141 taagctgatt taatattgga attaccgcag aaccggctgg aataatcaca ttttcccgca
    28201 aatcggaaag tcgatatggt ctagcacttc cctttttata aacaagctgc caagaagccg
    28261 aataatagga aggacctatc tgaaattcat cgtttaattc agaatggtaa agcagcccag
    28321 cggcagcaat atcaacatca ttatttctta aggcctcaaa caatttctcg ctactatcat
    28381 aagttttaat atcaagattg acctttaaat attgagcaaa ggctgttgct aaatcatatt
    28441 caatgccact ttttccttca ggaccaacaa agtaagaaag tggatggttg atcattccaa
    28501 cctttaacgt tttgctttgc tgaatttgtg tataatgatt tgcttctgaa tgaatgattt
    28561 tttgccaagg aaaaatcata tcccaagccc acagtaacaa tgctacggca ataaaaaggc
    28621 gagcaattaa tcctttcaat ttaaaatcct cattatttca agctaagcat taagcctatc
    28681 tgaaat
//
LOCUS       DANLRK010000010        28230 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.010, whole genome shotgun sequence.
ACCESSION   DANLRK010000010 DANLRK010000000
VERSION     DANLRK010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 28230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28230
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.010"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(23..862)
                     /locus_tag="PXA33_000305"
     CDS             complement(23..862)
                     /locus_tag="PXA33_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HDL5119679.1"
                     /translation="MQSYPYLLVNVFATSHFGGNPLAVFYEADGLTDLQMQLIARQFN
                     LSEVVFIQSSTDSQAVKKLKIFTPDYEMPFAGHPTVGAAFVLHSAFDFPTTYRLQTNA
                     GLVAIQHQHNVTTFALTNGVNLENTPLSRAECASILGLNVADIASEPCYVNTGVEQLL
                     VELASQQAVEKCKINANLAEKAFLKDSVYVWCREGNEAKVRLFFQSQGSIVEDPGTGS
                     AAANLGGWHIAQGKIPIQLQISQGDEIQRPNRLLLKVDQNGKIFVGGEVILVGKGEFY
                     LPE"
     gene            1052..2386
                     /locus_tag="PXA33_000306"
     CDS             1052..2386
                     /locus_tag="PXA33_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HDL5119680.1"
                     /translation="MLYAEFLLLLAFLYAGSRYGGIGLGVVSGIGLFVEVFFLGMPLS
                     APPISVMLVILAVVTCASILEAAGGLKFMLQVAERILRSNPKRITFLGPLVTYLMTLM
                     LGTGHSVYSIMPIIGDIALKNKIRPERPMAAASVASQLGITGSPLSAAVAYYLTEIVK
                     LPGFEGVTLLNVVGVTITATFCGVIAMSLYSLRRGKELEDDPEYQRRMQDPELRKQIE
                     QTSATSLDEKLPASAKNSVYLFLAAIATIVIIAMIPAIKPTVAATGKVAGMDQIIQIV
                     MLTFGGLILILTKTDPKKVPNGIVFKSGMVATIAIFGIAWMSDTYFTHAMPAFKEGVT
                     EMVRAQPWTFAFALFAVSVVVNSQAVTAKMLLPVGIAMGLPAPLLVGLMPATYAYFFI
                     PNYPSDIATVNFDVTGTTKIGKYYFNHSFMAPGLVGVITACIVGIAVANVLI"
     gene            complement(2466..2876)
                     /locus_tag="PXA33_000307"
     CDS             complement(2466..2876)
                     /locus_tag="PXA33_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HDL5119681.1"
                     /translation="MNSTLTNLQPLAFLITRVVAGYMFLLHGTAKFFEFPMSMTNGNG
                     SVPLASIYGVGGILEIVGGIFFILGLFTRQTAFILSGMMAYAFFIAHGAQGNVLLPLL
                     NGGELAGVYSVLFLTYIFIGGGKYSLDAKLFNKA"
     gene            complement(2999..3676)
                     /locus_tag="PXA33_000308"
     CDS             complement(2999..3676)
                     /locus_tag="PXA33_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HDL5119682.1"
                     /translation="MNLFGFRCFHCNHPLAIGVQGICSHCQKLLKPTPYCRHCGMSAV
                     EFRNACGYCLKNEPKWHKLVRVNEYRAPLSHWVHQFKFQQQYWWDQALARQLLLAIKQ
                     AQREQVLSLPEVIMPVPLFWHRHWKRGFNQSELLCDWLSKWLQIPLDTQSLQRVRATV
                     SQRELTAAERRKNLRGAFFYQPLKAYKRVAIVDDVLTTGSTLNVICAELLKAGVVEIQ
                     VWVLARA"
     gene            3794..4606
                     /locus_tag="PXA33_000309"
     CDS             3794..4606
                     /locus_tag="PXA33_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005610932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL5119683.1"
                     /translation="MKSLQKPIKIVFFDIDDTLYVKHKAYIPKSITEQVLPRLKAKGI
                     IPAIATGRNHGCFPEALKPYLNPEIGFELFVTINGQYNFYKDQIISDYSLSREQIERI
                     IEKANALGILYAFITPMTIAVSETNPIVHEAVLPITPNYLIDPHYYEKEKIVQMLLFY
                     TEEQKQAVENSGIFANDLKEARWHPNAVDILRKENSKARGIQDVLATLNIEIESAMAF
                     GDGINDLEMLPTVGVGVAMGNGVPELKAVADFITKPLEEDGILYALETLEVI"
     gene            complement(4654..4923)
                     /locus_tag="PXA33_000310"
     CDS             complement(4654..4923)
                     /locus_tag="PXA33_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDL5119684.1"
                     /translation="MGLLYLLLLNTLIGYGSYGEALNHWDTSKVSIVTTMLPIFTMIF
                     SNLSYYFYPHIFAKPDMNWISYLGALVVVSGAILAIAGDKLFRRN"
     gene            complement(4896..5573)
                     /locus_tag="PXA33_000311"
     CDS             complement(4896..5573)
                     /locus_tag="PXA33_000311"
                     /inference="COORDINATES: protein motif:HMM:NF013088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDL5119685.1"
                     /translation="MKQRPLLGFSLALTAAMTWGTLPIAAQQVLKVIDAQTLVWVRFV
                     VASVGLLLILGFAKRLPKLTACSKKELGLILLGVCGLSANFFLFAEALHYVSPTINQV
                     LWQLAPFSMILLGVLLFKEKFGLFQKIGFVLLIAGLVAFFNDKFAEILQLNTYALGVL
                     LSAGASLIWVLYGVAQKLLLAKFNSQQVLFIIYIGCSLLMLPFASPAQLGNLEDGFLW
                     GCFIYCC"
     gene            complement(5675..5932)
                     /gene="rpmA"
                     /locus_tag="PXA33_000312"
     CDS             complement(5675..5932)
                     /gene="rpmA"
                     /locus_tag="PXA33_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007369914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HDL5119686.1"
                     /translation="MATKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGSNVGMGKDHTLFATADGKVKFEVKGEKSRKYVSIIAD"
     gene            complement(5953..6264)
                     /gene="rplU"
                     /locus_tag="PXA33_000313"
     CDS             complement(5953..6264)
                     /gene="rplU"
                     /locus_tag="PXA33_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HDL5119687.1"
                     /translation="MYAVFQSGGKQHRVSEGQVVRLEKLELATGEKVEFDSVLMVVNG
                     EDVKIGAPVVAGAKVVAEVVAQGRGEKVKIVKFRRRKHSRKQQGHRQWFTEVKITGIQ
                     A"
     gene            6542..7540
                     /gene="ispB"
                     /locus_tag="PXA33_000314"
     CDS             6542..7540
                     /gene="ispB"
                     /locus_tag="PXA33_000314"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HDL5119688.1"
                     /translation="MAVQLFSLEQIQALANEDMQAVNAEILAQLNSEVLLINQLGHYI
                     IAGGGKRIRPLIAVLAARALGYRGKKHITAAAFIEFVHTATLLHDDVVDESDMRRGRP
                     TANAHFGNAASVLVGDFIYTRSFQMMTDLDSLRVLKVMSAATNALAEGEVQQLMNVND
                     PNTTEANYMRVIYNKTARLFEAATQCVAIAADASEEIETALKNYGCYLGTAFQLIDDI
                     LDYSAQSESLGKNVGDDFVEGKPTLPLLHAMHAADKAGKTTQAELIRNAIENGGELEQ
                     IPQVLAIMQEHQSLDYAMQKAREEAQKAVDSLGVLPESEYKQALVSLAYLSVDRSY"
     gene            7643..8389
                     /locus_tag="PXA33_000315"
     CDS             7643..8389
                     /locus_tag="PXA33_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HDL5119689.1"
                     /translation="MTEQNQRPQEHIESVRARRRAQNQKKGPNAPFVREKLTLPNGHN
                     KLLLHSCCAPCSGEVMEAIHASGIEFTIYFYNPNIHPLKEYLIRKEENIRFAEKWGIP
                     FIDLDDDYENDRKEWFKKAKGMEWEPERGIRCTMCFDMRFEKAAEYAHNNGFPVFTSC
                     LGISRWKDMNQINDCGHRAAAKFDDVVYWDYNWRKGGGSQRMIEISKRERFYQQEYCG
                     CVYSLRDSNKWRLETGRQRIEIGKLYYSPD"
     gene            8806..9741
                     /gene="fabD"
                     /locus_tag="PXA33_000316"
     CDS             8806..9741
                     /gene="fabD"
                     /locus_tag="PXA33_000316"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014990844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDL5119690.1"
                     /translation="MSKFAMVFPGQGSQAVGMLADLAPQYPIVEETFKQASDVLGYDL
                     WDLVQNGTAEELGQTHRTQPALLASSVALFRIWQQKFPEQKPAVMAGHSLGEYSALVC
                     AGVLDFQDAIKLVELRGRAMQEAVPAGSGAMFAIIGLDNESIIKACEQAASETGEIVS
                     AVNFNSPGQVVIAGTKAAAEKAGELCKAAGAKRALPLAVSVPSHCALMKPAAEKLAEA
                     LQNIELNQPLVPVINNVDVAVETDADTIRNALVRQLYSPVRWTETVEKMAQDGITTLY
                     EIGPNKVLTGLAGRIVKELSAKAVNDVASFEAVEA"
     gene            9758..10222
                     /locus_tag="PXA33_000317"
     CDS             9758..10222
                     /locus_tag="PXA33_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5119691.1"
                     /translation="MSKETLFMLISALIALVILPLAYTVGIAKETLGGNMGMLSIAAG
                     CSFLAYFLSLNPKIRHAFIGGSAITAAAIQAWVLYGVLLSENDPKSAVVLFQIYLFSS
                     LGPLFFSAIWAFTRPRWIYPKQGSAVKYALFGGLTTVINYLITLFIFYILTL"
     gene            10243..10965
                     /gene="fabG"
                     /locus_tag="PXA33_000318"
     CDS             10243..10965
                     /gene="fabG"
                     /locus_tag="PXA33_000318"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HDL5119692.1"
                     /translation="MSKIALVTGATRGIGKAIAEELVAKGYIVVGTATSEKGAESISA
                     YLGENGKGLVLNVADAESIESVLKQIKETFGDVDVLVNNAGITRDGLLMRMKDEDWFD
                     IIQTNLTSVYRLSKAVLRPMMKKGGRIITIGSVVGSMGNPGQTNYCAAKAGLVGFSKS
                     LAKEVASRGITVNVVAPGFIATDMTDELTEDQKNAILSQIPAGELGSPQDIAKAVAFL
                     ASDDARYINGETIHVNGGLYMS"
     gene            11084..12382
                     /gene="brnQ"
                     /locus_tag="PXA33_000319"
     CDS             11084..12382
                     /gene="brnQ"
                     /locus_tag="PXA33_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDL5119693.1"
                     /translation="MTRNTLVVGFLLFAIFFGAGNLIFPPKLGYESSTEFFSAISGFV
                     LTGVGLPLLGIIASAFYNGGYKSALDKIHPWFSLAFLTSIYLAIGPFFAGPRTAATAY
                     EMAIMPFLGDSSQVSMLIFTLIYFAFTLWLALNPNKMVDRIGAILTPILLITIIGLVI
                     RAGFILDTDPQITTPNTNEQSYFFKGMVDGYLTMDALASLAYSVIVLNAIRGKGVSQA
                     ALQRQTVSAGLVAAVALAVIYISLGWIGNNMPISANTLSMLSERKLDLGTYILNTVTT
                     QAFGELGRSVLGVIVSLACLTTAVGLAVSVSEYFNEIFPKISYKVYAILFTVISFTVA
                     NQGLKEVISKSIPVLLVLYPIAMTVILMLLINLVIKLPLSAQRLSLLLVTIVAIASVA
                     GVGFTENLPLKSYSMEWLPFAIIGAIIGAIIHPLTGRKHK"
     gene            12428..13252
                     /gene="mutM"
                     /locus_tag="PXA33_000320"
     CDS             12428..13252
                     /gene="mutM"
                     /locus_tag="PXA33_000320"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005713993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HDL5119694.1"
                     /translation="MPELPEVETSVRGVSPYLVGQTIEQIIVRQPKLRWTVSPELSQM
                     AGAKITAVERRAKYLIIRTDKGDILVHLGMSGSLGILQERQQKEIGKHDHVDLITQNG
                     TILRYNDPRKFGCWLWAEKAEDHELLRKLGPEPLSNDFTADYLFAKSRKKTTACKTFI
                     MTNEVVVGVGNIYACESLFMAGIHPELAAQNLTKKQCERLVKTIKEVLTKAIIQGGTT
                     LKDFIQPDGKPGYFAQVLQVYGRKGETCNDCGKPIETKVIGQRNTYFCPKCQRLPK"
     gene            complement(13335..13769)
                     /gene="dtd"
                     /locus_tag="PXA33_000321"
     CDS             complement(13335..13769)
                     /gene="dtd"
                     /locus_tag="PXA33_000321"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HDL5119695.1"
                     /translation="MIGLIQRVKFAKVEVEGQVVGEIASGLLVLLGVEKDDDEAKADK
                     LLEKVLNYRVFSDSEGKMNLNVQQAGGSLLVVSQFTLAADTNKGLRPSFSKGASPVDA
                     ERLYAYFHQQAATKIHTQTGQFAADMQVSLQNDGPVTFWLQV"
     gene            complement(13744..14589)
                     /locus_tag="PXA33_000322"
     CDS             complement(13744..14589)
                     /locus_tag="PXA33_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HDL5119696.1"
                     /translation="MLNRLWRFIKLFAKRYNENNIAISAGYLTYSTMLAIVPFIVVLF
                     SIFTFFPIFHEANEQLKAFIYDNFAPSASEMVSEYIELFVSNAKQMGLVSFIGLFVVT
                     IMLIKSIDEALNTIWHDNRQRPMWFSFLLYFLILLFGPLLAGASIAISTYVMSLSIFN
                     GNGALSLGQHLLELMPFLLIWLLFTLVYKFIPIRKVKFQHAAIGALMAAIFFTLGKKA
                     FIWYITAFPSYQAIYGALAVLPIMLLWIQLSWQVVLLGGQFAAVLDEMETDKGSINDR
                     LNSAG"
     gene            complement(14591..15829)
                     /gene="proA"
                     /locus_tag="PXA33_000323"
     CDS             complement(14591..15829)
                     /gene="proA"
                     /locus_tag="PXA33_000323"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HDL5119697.1"
                     /translation="MQMIELAQQAKQASFELAQFGNLQKNQALLTIADSLEQRAEEIL
                     YANAKDIEFAQAQGISLAIIDRLLLNEDRLKDIANDVRNVAKLADPVGQVMDGGVLDS
                     GLKIERQRVPLGVILTIYEARPNVTIDVASLCLKTGNAVILRGGKETKFTNAVLVDVV
                     QDALEKAGLPKLAVQAVTDPDRALLLELLKLDRYIDMVIPRGGAGLHQFCKENSTIPV
                     IVGGIGVCHLFVEKTADQEKSLEVIANAKTQRPSTCNTLETLLVEKAIAVEFLPKLVA
                     KMQQLGVTIHSNDLQKIEGIEPLDQAKLSQEWLSLDLNVVVVNDLQQAVEHIRHYGSQ
                     HSEGILTSDYQLARQFVQQVDAAAVYINASTRFTDGGEFGLGAEVAVSTQKLHARGPM
                     GLEALTTYKWVCEGDYLVRG"
     gene            16113..17996
                     /gene="mutL"
                     /locus_tag="PXA33_000324"
     CDS             16113..17996
                     /gene="mutL"
                     /locus_tag="PXA33_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HDL5119698.1"
                     /translation="MINTTPKQLIHILPPQLANQIAAGEVVERPASVVKELVENSLDA
                     GANTIQIDIEKGGSQLIRIRDNGCGISKQDLPLALARHATSKISSLEDLEAILSLGFR
                     GEALASISSVSRLTLTSRTAEQTEAWQAYAQGREMAVEIQPASHPIGTTIEVANLFFN
                     TPARRKFLRTDKTEFAHIDEVVRRIALAKPNISFSLSHNGKIVRQYRKVQDNSVEQQQ
                     KRVAAICGDEFIQHSTYIDRQHGDLHLHGWVGSPALARLQNDLCYSYVNGRMMRDKTI
                     NHAIRQAYGDSIASGYYPAFVIFLDLDPTMVDVNVHPAKHEVRFHQGRLVHDFILQGV
                     LNSLQQTQPTLELRNEVNEPLPSYVQDTNRQAAGQNIFVSQTAPQAAEFTQKNASRSS
                     YTPKFERSNSVSSSAQKWYKELVGGDLHQTTPTIKTQSTLVEPKTEPQTEHKTEAVSQ
                     KITQIAPLASHHSQALAIVKNQAVLIKEEENFYLLPLATLAELKLKLQLEKGQSQALL
                     IPLNLSLDQQQTERWQQTKEELTKLGFSISEKQWQAQTRLTVLSVPQAVREQNLQQLL
                     FALFNQTQAVNLAEFFAKKCEKPTACTLSDVIALLSEIELYANGKKELEGIRKEVDFS
                     KFL"
     gene            complement(18015..18284)
                     /locus_tag="PXA33_000325"
     CDS             complement(18015..18284)
                     /locus_tag="PXA33_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin, RelE/StbE family"
                     /protein_id="HDL5119699.1"
                     /translation="MREIEYSNAFKRDYKKCAVESLSSSLIEALYFLINDKPLPEKYC
                     DHALTGEWKGFRDCHIQPDLVLIYRKVEDDKLELLRIGSHSHLFG"
     gene            complement(18271..18537)
                     /locus_tag="PXA33_000326"
     CDS             complement(18271..18537)
                     /locus_tag="PXA33_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HDL5119700.1"
                     /translation="MANSALVSVRISPEIKEQASEVLAGIGLTVSDVMRMTLAKIATE
                     KRFQFDYQPNFETAEVLRAVKNGEEKLHSANSIADLMEQLNARD"
     gene            18977..19930
                     /gene="miaA"
                     /locus_tag="PXA33_000327"
     CDS             18977..19930
                     /gene="miaA"
                     /locus_tag="PXA33_000327"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HDL5119701.1"
                     /translation="MNKKPLALFLMGPTASGKTDLAIALRQELPVEVISVDSALIYKG
                     MDIGTAKPSKEELALAPHRLIDILDPAESYSAMNFHADALREMADITSQGKIPLLVGG
                     TMLYYKALLEGLSPLPSADPAIRAEIESRAAQHGWATLHQELVKIDPIAGARINPNDS
                     QRINRALEVFYITGKTMTELTAQQGEALPYNILQFAIAPQDRAVLHQRIEQRFHKMIE
                     LGFEEEVKRLFLRKDLHINLPSIRCVGYRQMWEYLQGDISLDEAIFKGICATRQLAKR
                     QITWLRGWQGELTWLDSLAPTSSKAKMVEKIDHYLKNCDNI"
     gene            19999..20271
                     /gene="hfq"
                     /locus_tag="PXA33_000328"
     CDS             19999..20271
                     /gene="hfq"
                     /locus_tag="PXA33_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005712219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HDL5119702.1"
                     /translation="MAKGQSLQDPYLNALRRERIPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPARALSHNNNNQAQHAQPAAEVTEQAE"
     gene            20291..21676
                     /gene="hflX"
                     /locus_tag="PXA33_000329"
     CDS             20291..21676
                     /gene="hflX"
                     /locus_tag="PXA33_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HDL5119703.1"
                     /translation="MLNESLPNSEENSAFGFALSEASQTTEAASPTDPENQDKAILVH
                     LYLGQHKDVENLLEFKTLAESAGVEVLATLTTSRQSSHIKYYVGQGKAEGIAEAVERL
                     GATVVLVNHQLSPAQTRNLQSLCGCRVVDRTGLILDIFAQRARSHEGKLQVELAQLRH
                     LSTRLVRRVTNQDQQKGGAVGLRGPGETQLETDRRLIKVRIQQLLNRLEKVSKQRNQN
                     RKTRQKADIPTVSLVGYTNAGKSTLFNAITQAGVYAADQLFATLDPTLRRIQVQDVGT
                     TILADTVGFIRFLPHDLVSAFKSTLQETTEATLLLHVIDGSDERKNENIDAVNQVLDE
                     IGALDIPTLLVFNKIDKLEGVEPHIERNADGTPVAVYLSAQENSGIELLFQAIQERLR
                     SNLVCENLLLPVTAGAIYAQFKAENCIKTEHFNEFGERIISIEINEVQWNKWVKQFPD
                     LAEYIELAKWG"
     gene            complement(21898..27969)
                     /locus_tag="PXA33_000330"
     CDS             complement(21898..27969)
                     /locus_tag="PXA33_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HDL5119704.1"
                     /translation="MNKIFKVIFNQTTQKMEVVSELARSQGKAASSTDKRGTIKLAAA
                     SILGGATLISGMAAFMLSATPAEAAVIIDRIEVQQKAPKKVVNGGIDIYTNTSLATDS
                     ITNPYNYFNPGSLSYADRTKLQESNNSVNKEKDSRLLYKSNTTSAIVIGHNAVALDNN
                     RKSRGKPKASGIAIGDYSYSGGGLSIAIGSFSRTASIGSVALGTSARAEGFNSFAAMR
                     QSAAIADYSTAIGSTSWANATASFAIGASATALGDRSFAIGSGTPQTVDGSYGHENRR
                     SIYDGQNNTLAFGTDAFSIGTKAKTNGNHSFAIGTNAWSGGFKLENSNISELGQVINP
                     DTAKVANNAFALGTNTRANSNDTLAFSTNASATGDGSIAFGVNSNTTGIGAIAFGNAS
                     KAWKSNAISIGWNANSHAEKVISIGSHSNASKDGAMAIGDTANSSGNNSVAIGYSANV
                     SKANATAVGTRANITHEGGTAIGFEAKSKGNYATAIAYNSTAKGKNSIAAGVNSTVSG
                     IRAAALGYNNTVSGKDTFVLGSNVTVKTKGSVILGSDSTEATATKEDTATVGTLKYSS
                     FAGDENVVDGDYVSVGNATNARQIKFVAPGKIASNSTDAINGSQLYAVADTLVKTGFN
                     ITADHSGLGGGAKEDNVKLGETVKYTSTDKNVVTTVTNNTIDFGLSDNISVGGKPGKD
                     GSVGVKGADGKDGVTIKPEAIVFHGVDGVNGKDGKDGKASIKVEKGAKGLDGNDGKDG
                     ESKTRIVYEKPNGDKEEVATLNDGLKFGANDGKVHDAKLNTQVDIKGATTNTDWAKFD
                     AGKNIMTQVVGNTITVALAKDLAGLDSATFGTQGDSTVINKDGVTIAKKDPNDATKSN
                     VTLTEDGLSNGGNQITNVDSGLKGKDGQPVKLTEAAGDVLNNAVNVGDLKNTVTETVT
                     EVTTDAFGLKDENNKEFKQNLGTTAKIIGDGNVKTNVVEKDGGHALEVSLVNNITVGK
                     DGEPGKVGIAGKDGKDAIALDGKDGAITVGRPGKDGIDGTIGVNGKDGSAVVLNGKDG
                     SIGLNGTNGKDGLTIKGTEGKPGLVGKDGLPGKDGVTRIQYQPTDGNGDPIGEPEQVA
                     TLNDGLHFKGDDATLVSKKLNETLEIIGGADKDKLTANNIGVNSKDGKLSVQLAKDLK
                     DLNSATFKPQDGENGQEGKGPTTVVNNNGVTITPKDGTDGKAVKLTEDGLDNGGNQIV
                     NVKSGIDGLAGKDGINGKDGKPITSVNDLTDDQLKEVGTNAANINDVVNASNDIIEKG
                     FSIAADNGETDHVKLGETVKYTSKDKNIITTVSDNVIDFTLNNDFTIGGPGKDGSIGV
                     KGADGTVGVTLNGKDGSIGLTGPKGADGKDGASATISVKDGAKGLDGNDGKDGESKTR
                     IVYEKPDGTTEEVATLNDGLRFVGDDGKEIVKKLNETLKISGGDTDLTNLSDNNIGVV
                     NNNGALTVKLAKDIDLTKDGSVTIGDTTVNNDGLTIKDGPSVTKAGINAGDKKIINVQ
                     DGEISATSKDAVNGSQLYQVQEVANAGWNLTANGKDKGNVKPGATVDLNNKDGNIIIT
                     KEGNNVTFGLKNNLTIGGKDGKDGQIGIAGKDGKDGVTIKGDGTITAGRDGKDGVDGS
                     IGATGKDGASVVLNGKDGSIGLTGPKGADGKDGASANIAVKDGAEGVDGTNGKDGLPG
                     ENGKTRIVYETKDKDGKTVTEQVATLNDGLIFSGNNEDVKNRQKLNTEVKVKGEGVDK
                     TASENFKSASGNINVKANGTDTLEVQLAKAIDLTEDGSVTTGDTTVNNNGLTIKDGPS
                     VTKGGIDAKGTKITNVKDGDVTATSKDVVSGSQLYNAMQNTGWYLTVDGNVAETSEKG
                     AQRVNNNGKVAVSGGKNIVVSRKGSTVEVATSSNLEFDSVKVGGTTISSTVAKDGVNE
                     LNIAGSKNAQTRITNVAPGVKGTDAVNVNQLKGAENRLNNRISNVDKDLRAGIAGALA
                     AGNLYHVTQPGKSMVSAGVGAYRGQGAIAVGYSRLSDNGKVGIKFSVNSNSRGDTGAA
                     ASVGYQW"
BASE COUNT         8256 a         6341 c         5517 g         8116 t
ORIGIN      
        1 agcaaacaga atttgcttac cctcattcgg gtaaataaaa ctcgcctttg ccgactaaaa
       61 taacctctcc gccaacaaaa atcttcccgt tttgatcgac ttttaataac aggcgattcg
      121 gccgttgaat ttcatcacct tgtgagatct gaagctggat tggtattttg ccttgtgcaa
      181 tgtgccaacc gcctaaattt gccgctgccg aaccggtgcc ggggtcttcg acaatcgaac
      241 cttgcgattg aaaaaacagt cgcactttcg cctcatttcc ctcacgacac caaacatata
      301 cactgtcttt caaaaatgct ttttcagcca gatttgcatt tattttgcac ttttcgaccg
      361 cttgttgaga agctaactca accaataact gctccacgcc ggtgtttaca taacaaggct
      421 cggacgcaat atccgcaaca ttcagcccca atattgaggc acattctgca cggcttagcg
      481 gtgtgttttc caggttcaca ccattggtta aagcaaaagt agtcacatta tgttgatgtt
      541 gaatcgctac caatccggca ttagtttgca agcgataggt tgttggaaaa tcaaaggcgg
      601 aatgcaacac aaaagccgct ccaacagttg ggtggccggc gaacggcatt tcataatcag
      661 gcgtaaaaat tttgagtttt ttgaccgctt gtgaatcggt ggaagattga ataaatacca
      721 cttcagaaag gttaaattgg cgagcaataa gttgcatttg taagtcggtt aatccgtccg
      781 cttcataaaa tactgcaagc gggttgccgc caaaatggct tgttgcaaac acattcacca
      841 gcaaataagg atagctctgc atttatcact ccttacataa acttaaagcc acattgggcg
      901 aaaattctac cattttggtg gcgaatcgta aaattggaaa atttatcggt atccctcttt
      961 agagttaact atttttgatt tatctcaaag ttttaaaaaa aagattaggt catacttctt
     1021 tctagtgatt attttttaat ttgaggttct tatgctttat gcagaatttt tacttttatt
     1081 agccttttta tatgcaggta gccgttacgg tggcatcggt ttaggtgtgg tttccggcat
     1141 cggcttattt gttgaagtgt tcttcttagg aatgccgctt tccgctccgc caattagcgt
     1201 gatgctcgtg attttggctg tagttacttg tgcctccatt ctagaagccg ctggcggttt
     1261 gaaatttatg ttgcaagttg ccgaacgcat tttgcgtagc aacccaaaac gtattacctt
     1321 tcttggccca cttgtcactt atctgatgac cttaatgctc ggcacagggc attcggttta
     1381 ctctattatg ccgattatcg gtgatattgc cttaaaaaat aaaattcgcc cggaacgccc
     1441 gatggcagca gcctctgttg cttcacaatt aggtattacc ggtagcccac tttctgctgc
     1501 agttgcctat tacttaaccg aaatcgttaa attaccgggc tttgaagggg tgactttatt
     1561 gaatgtagtc ggcgtaacca tcaccgcaac cttctgtggt gtgattgcga tgtcattata
     1621 tagcttacgt cgtggtaaag agttggaaga cgatcctgag tatcaacgcc gtatgcaaga
     1681 ccctgagcta cgcaaacaaa tcgagcaaac gagtgcaacg tctttagacg aaaaactacc
     1741 ggcaagtgcg aaaaactcgg tttatctgtt cctagccgct attgcaacca ttgtgattat
     1801 tgcaatgatt ccggctatta aaccaacggt tgctgctaca ggaaaagttg ccggtatgga
     1861 tcaaatcatt caaatcgtga tgctaacttt cgggggttta atcttaattt taactaaaac
     1921 cgacccgaaa aaagtaccaa acggcatcgt tttcaaatcc ggtatggtag caaccattgc
     1981 gattttcggg attgcttgga tgagtgatac ttacttcacc catgcaatgc cagcgttcaa
     2041 agaaggggta acagaaatgg ttcgagccca accttggaca ttcgccttcg ccttgtttgc
     2101 ggtatctgta gtagtaaaca gccaagccgt aaccgcaaaa atgttattac cggtaggcat
     2161 tgcaatgggc ttgcctgctc cattattagt tggtttaatg ccggcaactt atgcttactt
     2221 ctttattccg aattatccgt ctgatatcgc aaccgttaac tttgacgtaa ccggtacaac
     2281 taaaatcggt aaatattact tcaaccacag ctttatggct ccgggtttag ttggcgtaat
     2341 tactgcctgt attgtcggta tcgccgttgc aaatgtgcta atttaatttt aacaaaaaaa
     2401 caagcggtca gattaagtaa attttttacc aaatctgacc gcttgattat ttactgcaaa
     2461 accaattagg ctttgttaaa gagttttgca tctaacgagt attttccgcc accgataaaa
     2521 atataggtta agaataatac cgaatacacg cctgctaatt caccgccgtt taataaaggt
     2581 aatagcacat tgccttgagc tccgtgagca ataaagaacg cgtatgccat catacctgaa
     2641 aggataaacg ctgtttggcg ggtaaataga cctaaaataa agaaaattcc gcccacaatt
     2701 tccaaaatac cgcctacacc ataaatggag gcaaggggta cactgccgtt tccgttggtc
     2761 atcgacattg gaaattcaaa aaatttcgct gtgccgtgta ataaaaacat ataaccggca
     2821 acaacacggg tgattaaaaa cgctaaaggt tgtaaatttg ttaaagtaga gttcatttgc
     2881 tttccttaaa ataaattgtg atataaaggt caattgattg aggcaggcat tctattttaa
     2941 aaacctatac ttcacaatcc agccgtcatg aaatagatta tttaccatta ataaacaatt
     3001 aggctctcgc taacacccaa acctgaattt ctactactcc agctttgagt aactcggcac
     3061 aaattacatt taaggtggaa cctgtggtga gcacatcatc aacaatcgcc actcgtttat
     3121 aggcttttaa cggttgatag aaaaatgccc cacgcagatt tttccgccgt tcagcagcgg
     3181 tcaattctct ttgtgagacg gttgctctca cacgttgtaa actctgggta tccagcggaa
     3241 tctgtaacca cttcgataac caatcacaca ataattcgct ttgattaaac cctcttttcc
     3301 aatgtctgtg ccaaaaaagt ggtacaggca taatcacttc aggcaagctc agaacctgtt
     3361 ccctttgagc ttgtttgatt gccagcagca attgtcgggc aagggcttga tcccaccaat
     3421 attgctgctg gaacttaaat tgatgtaccc aatgtgaaag tggcgctcgg tattcattta
     3481 ccctcactaa tttatgccac ttcggttcat ttttcaaaca atagccacaa gcgtttcgaa
     3541 actccaccgc actcattcca caatgacgac aataaggggt tggttttagc aatttttggc
     3601 aatggctgca aatgccttgc acgccaatag cgagtggatg attacaatga aagcaacgga
     3661 agccgaataa attcacattt cctcctttta tttgcagatt atcaaattta gccttatttt
     3721 ttccggctat ttttgtgaca tcgctcgaaa aaagagtaaa atctcattat cagtaaataa
     3781 aaaggaaacc gagatgaaat ccctccaaaa accgatcaaa attgtctttt tcgatattga
     3841 tgacaccctc tatgtaaaac ataaagccta tattccaaaa tcaattaccg agcaggtatt
     3901 acctcgttta aaagccaaag gcattattcc ggccattgcc accggcagaa atcacggctg
     3961 ttttcctgaa gctctcaaac cttatttaaa ccctgaaatc ggttttgagc tgtttgttac
     4021 cattaacggg caatataatt tctataaaga tcaaataatt agtgattatt cgttaagccg
     4081 tgagcaaatt gaacgtatta tcgaaaaagc gaatgctttg ggtattctgt atgcctttat
     4141 tacgccaatg acgattgcag tttccgaaac caatccgatt gtgcacgaag cggttttacc
     4201 gatcacaccg aattatttga tcgatccaca ctactacgaa aaagagaaaa ttgtgcaaat
     4261 gttactgttc tacaccgaag agcagaaaca agcggtcgaa aatagtggca tttttgcaaa
     4321 tgatttaaaa gaggctcgct ggcaccccaa tgcggtagat attctgcgaa aagaaaattc
     4381 gaaagctcgt ggcattcaag atgtattagc aaccttgaat attgagattg aaagtgcgat
     4441 ggcatttggc gatggtatta acgacttgga aatgctcccg accgtaggtg ttggtgtggc
     4501 aatgggtaat ggcgtgccgg aattaaaagc ggtggcagat ttcatcacca agccgcttga
     4561 ggaagacggc attctatacg ctcttgaaac tcttgaggtg atttaaaagc aagcggttgt
     4621 aagtttttct ccatttacaa ccgcttgtag tttctaatta cgcctaaata gcttatcgcc
     4681 tgcaatcgct aaaatcgccc cactgacgac caccaacgcc ccaagataac tgatccaatt
     4741 catatccggc ttggcaaaaa tatgcggata aaaatagtag ctaaggttgg aaaagatcat
     4801 cgtaaaaatc ggcaacatcg tggttacaat gctaaccttt gaagtatccc aatgattcaa
     4861 cgcctcaccg tatgaaccgt agccaattaa ggtgttcaac agcaataaat aaagcaaccc
     4921 cacagaaaac catcctcaag attgcctaac tgtgccggtg aggcaaacgg taacatcagc
     4981 agagaacagc cgatgtaaat aataaacagc acttgctgag agttaaattt cgctaataat
     5041 aatttttgag ccacgccgta tagcacccaa atcaagctgg ctccggcact caataacact
     5101 ccgagagcgt aggtgttgag ctgtaaaatt tcagcaaatt tatcgttaaa aaatgcgaca
     5161 agccctgcaa tcaataacac aaatccaatc ttttgaaaca agccgaactt ttccttaaac
     5221 aataatacgc ctagtagaat catcgaaaat ggagctaact gccacaatac ttggttaatg
     5281 gtgggcgaaa catagtgtag agcctctgca aacaaaaaga aatttgccga cagaccacac
     5341 acccctaaca aaattaaacc gagttctttt ttgctacaag cggtcaattt gggtaaacgt
     5401 tttgcaaatc ctagaatcag caatagccct actgatgcca caacgaacct aacccaaact
     5461 aaagtttggg catctatcac tttcaatact tgctgggcgg caatcggtag cgttccccag
     5521 gtcattgctg cggttaatgc aagtgaaaag ccaagtaagg ggcgttgttt catcgtggaa
     5581 attccttttt ctattctatg tttctatatt ttagaaagat tgagatacaa aaaaaccccg
     5641 ccattggcga ggtctcttat tgattttgaa atgattagtc agcaatgatg cttacatatt
     5701 tacggctttt ctcgcctttc acttcaaatt taactttacc gtcagcagtt gcaaataaag
     5761 tgtggtcttt acccatacct acatttgaac ctgcgtggaa tttagtacca cgttgacgaa
     5821 cgataatgct acctgctaat acagattcac caccgaaacg tttaacacca aggcgtttag
     5881 cttcagaatc acgaccgtta cgagttgaac caccagcttt tttagttgcc atcttctaga
     5941 tctcctctga aattatgctt gaatcccagt gattttcact tctgtgaacc actgacgatg
     6001 accttgttgt ttacggctgt gtttacgacg acggaactta acgattttaa ctttctcgcc
     6061 acgaccttgt gctacaactt cagccactac ttttgcacct gctacaaccg gtgcaccgat
     6121 tttaacatct tcaccgttaa cgaccattaa cactgagtca aattcaactt tctcaccagt
     6181 tgcaagttca agtttttcta aacgaactac ttgaccttca cttactcggt gttgtttgcc
     6241 gccactttgg aaaactgcgt acatatttac tccgctatgt gccaactgct tttgctttta
     6301 ggcactaaat taattcataa aaatagggtt gcaattctac ggaaaaagat tcgaaagagc
     6361 aagagcgatt ttgagaattt tttgaaattt gcaaaaaatg gtaaaaatac taccgcttgt
     6421 ttcaactgcg actaaaaaac aatagactgc tccgatttta caacaaaatc ataattcttt
     6481 tttgaacaca ggctaaatta aggtagaatc tactcaattt tcgtcttact ttatggattc
     6541 gatggctgtg caactttttt ctttagaaca aattcaagcc cttgcgaatg aagatatgca
     6601 agcggtaaat gccgaaattt tagctcaact taattccgaa gtgctactga ttaaccagct
     6661 cggacattat atcatcgcag gcggtgggaa acgtattcgc ccgttaattg cggtgcttgc
     6721 ggctcgtgcc ttgggttatc gtggtaaaaa gcatatcacg gcggctgctt ttattgagtt
     6781 tgtgcatact gctacacttt tgcacgatga tgtagtcgat gaatccgata tgcgtcgtgg
     6841 cagaccaacc gcaaacgccc attttggcaa tgcggctagt gtattggtag gggatttcat
     6901 ctatacccgt tcgttccaga tgatgacgga tttagactct ctgcgtgtgt taaaagtgat
     6961 gtctgccgcc accaatgcgt tggcagaagg ggaagttcag caattaatga acgtgaacga
     7021 cccaaacacc actgaagcga actatatgcg ggtcatttac aataaaaccg cccgcttgtt
     7081 tgaagccgcc actcaatgcg tggcgattgc ggctgatgct tctgaggaaa ttgaaaccgc
     7141 attaaaaaat tacggttgct acttaggcac ggcattccag ctgattgacg atattttaga
     7201 ctactccgct caaagcgaaa gtttaggcaa aaacgtaggc gatgactttg tggaaggtaa
     7261 gccaaccttg ccattgctcc acgcaatgca cgctgccgac aaagcaggca aaactactca
     7321 agcagagctg attcgcaatg ctatcgaaaa cggcggcgaa ttggaacaaa ttccacaagt
     7381 attggcaatt atgcaagaac atcaatctct ggattatgcc atgcaaaaag cccgtgaaga
     7441 agcccaaaaa gcggtggatt cattaggggt tttaccggaa agcgagtaca aacaagcctt
     7501 ggtttcattg gcgtatttat cggtggatcg ctcttattaa tttgcaaaaa atcatataaa
     7561 tttaaccact tgtaggggca aatgatattt gccccataac ctataattct tttgggcaaa
     7621 tgttatttgc cccaacataa caatgactga acaaaaccaa cgaccccaag aacatattga
     7681 aagcgtacga gcgagacgcc gtgctcaaaa tcagaaaaaa ggcccgaatg ccccgtttgt
     7741 gcgtgagaaa ttaacgctgc caaacggaca taacaaatta ctgctgcact cttgctgtgc
     7801 cccttgctcg ggtgaggtaa tggaggctat tcacgcctcc ggcattgagt ttacgatcta
     7861 tttttataac ccgaacattc acccgctgaa agagtatttg atccgcaagg aagagaatat
     7921 tcgctttgcc gaaaagtggg gtattccgtt tatcgattta gacgatgact acgaaaacga
     7981 ccgcaaagag tggttcaaaa aagccaaagg aatggaatgg gagccggaac gaggcattcg
     8041 ttgcacaatg tgctttgata tgcggtttga aaaagcagcg gaatacgctc acaacaacgg
     8101 tttccctgta tttaccagtt gcttaggcat ttcccgatgg aaagatatga accaaatcaa
     8161 cgactgtggt caccgtgcag cagccaaatt tgatgacgtg gtttactggg actacaactg
     8221 gcgtaaaggc ggcggctcac aacgaatgat cgaaatcagc aaacgtgaac gtttctacca
     8281 gcaagaatac tgcggttgcg tctattcgtt aagagatagc aacaaatggc ggttggaaac
     8341 agggcgtcag cggattgaaa tcggtaagct ctattactca cccgattaaa tgttactttt
     8401 ctttgcttcg ccaaagaaaa gtaaccaaaa gaaaggcgac ccgaacttcg cttagttacg
     8461 atttccttaa cggaaaattt gtaaactcgc tacgctcaaa cagtacaaat tttcctagaa
     8521 atcgcaagtc gctcagacgg gacaagcggt acattattca aaatttttta caaaatatag
     8581 tgatgtactc ttcccttctg aaccgcccga acgtgtttga attggtagga aaatttactc
     8641 tgagcgaagc gaatgtaaat tttccgtgaa accaattcaa actaagtgag gataagcggt
     8701 gaagtagggg tgtttctttg gttactttct ttgcacatgc aaagaaagta acgaaaactc
     8761 agatatagat ttcaacttaa aaatttacag aaggaataat aacaaatgtc aaaattcgct
     8821 atggttttcc ccggtcaagg ctcacaagcg gttggaatgc ttgccgacct tgcgccacaa
     8881 tatccaatcg tggaagaaac cttcaaacaa gcaagtgatg tgcttggcta tgacttatgg
     8941 gatttagtgc aaaacggtac tgccgaagag ttagggcaaa cccaccgcac tcaacctgcg
     9001 ttgcttgcct caagcgtggc acttttccgc atttggcaac aaaaattccc tgagcaaaaa
     9061 ccagccgtga tggcagggca tagtttgggt gaatactcag cgttggtgtg tgccggtgtg
     9121 ttggatttcc aagatgcgat taaattggtc gagttgcgtg gtcgtgcaat gcaagaagcc
     9181 gtgccggcag gcagcggtgc gatgtttgcg attatcggtt tagataacga aagcattatt
     9241 aaagcgtgcg agcaagcagc aagcgagacc ggtgaaattg tctctgcggt aaactttaac
     9301 agcccggggc aagttgtgat tgccggcaca aaagcagcgg cagaaaaagc aggagaattg
     9361 tgtaaagccg caggtgcaaa acgtgcgtta ccattagcgg tgagcgtacc ttcacactgt
     9421 gctttaatga aaccggcggc agaaaaatta gccgaagcct tgcaaaatat tgagctaaat
     9481 caaccgcttg tccctgttat caacaacgtg gatgtggcag tggaaacaga tgccgatacc
     9541 attcgtaatg cacttgttcg ccaactttac agccctgttc gttggacgga aaccgttgaa
     9601 aaaatggcac aagatggcat cactacttta tatgaaatcg ggccaaacaa agtcctaacc
     9661 ggtttagcag gtcgtattgt aaaagaatta tcagccaaag cggtaaatga tgttgccagc
     9721 tttgaggcgg ttgaggctta attttcggga gcgtaaaatg agtaaagaaa ccctatttat
     9781 gctaataagt gcattgattg ccttagtgat tttaccttta gcttatactg ttggtatcgc
     9841 taaagaaaca ttaggcggaa atatgggaat gttatccatt gctgccggtt gctcattttt
     9901 agcctatttt ttaagtctaa acccgaaaat tcgccacgcc tttatcggag gttcagctat
     9961 tacagctgct gctattcaag cttgggtatt atatggtgtg ctattaagcg aaaatgaccc
    10021 caaaagtgcg gtcgttttat ttcaaattta tctgtttagt agtttagggc cactgttttt
    10081 ttcagctatt tgggcattta ctcgcccacg ttggatttac cctaaacaag gtagtgcggt
    10141 gaaatatgcc ttatttggtg gcttaacgac cgtgataaac tacttgatta ccctttttat
    10201 tttttatatt ctgacgttat aacgtcatac taaggagaat ttatgagcaa aatcgcatta
    10261 gttacagggg caacccgtgg aatcggcaaa gcgattgccg aagaattggt agcaaaaggc
    10321 tacatcgtag tcggcaccgc aacctctgaa aaaggggcgg aatctatttc agcttattta
    10381 ggcgagaacg gcaaaggctt agtgctaaat gttgccgatg cagagtcgat tgagagcgta
    10441 ttaaagcaga tcaaagagac ctttggcgat gtggacgtgt tagtcaataa cgccggcatt
    10501 acccgtgacg gcttattaat gcgtatgaaa gatgaggatt ggttcgacat cattcaaact
    10561 aacttaactt cggtttatcg tttatccaaa gcggtattac gcccgatgat gaaaaaaggc
    10621 ggacgcatta tcacgatcgg ttctgtggtc ggctcaatgg gcaacccggg gcaaaccaac
    10681 tactgtgctg ccaaagcagg cttagttggt ttttctaaat ctttagcgaa agaagtagca
    10741 agccgcggca ttacggtaaa cgtggttgct ccgggcttta tcgccactga tatgaccgat
    10801 gagctaaccg aagatcagaa aaatgcgatt ttaagccaaa ttcctgccgg tgaactcggt
    10861 tctccacaag atattgccaa agcagtcgct ttcttagcca gcgatgatgc ccgttatatc
    10921 aacggtgaaa ccattcacgt taatggcggt ttatatatga gctaatccgc tacaagcggt
    10981 cttttttcaa ccgagctttg taaattcaga gttcggtttt gttatttgtt attttgtgtt
    11041 atcacctgct ttataggtat aggtgaaaaa gtgtgaatat gttatgacta gaaatacatt
    11101 agtagtaggc tttttattgt ttgctatttt ctttggtgcc ggcaatctga ttttcccacc
    11161 aaaattaggt tatgaaagta gtacagaatt tttctcagcc atttcaggct ttgttttaac
    11221 tggagtaggg cttcctctac tcggtattat cgccagtgca ttttataatg gaggctataa
    11281 atctgcatta gacaaaatcc acccttggtt ttctttagca ttcctaactt ctatctattt
    11341 agcgatcggt ccattctttg ccggtccgcg cacggcagca actgcctatg aaatggcgat
    11401 aatgccgttt ttaggggatt ccagccaagt gtcaatgtta atttttacct taatttactt
    11461 tgcctttacc ctatggttag cgctaaatcc aaataaaatg gttgatcgca ttggtgcaat
    11521 tcttaccccg atcttgctta taaccatcat tggattagtt attcgagccg gctttatttt
    11581 agataccgat ccacaaatca ctacgcctaa tacgaatgaa caatcttact tttttaaagg
    11641 tatggttgat ggctatctaa ctatggatgc acttgcttcg ttagcctatt cggtaattgt
    11701 attaaatgct attcgtggga aaggggtaag ccaagccgca ttacaacgcc aaacagtttc
    11761 tgccggctta gttgcagcag ttgctctagc tgtaatttat atttcattgg gttggattgg
    11821 taataatatg ccaattagtg caaacacact ttcgatgtta tcagaaagaa aattggatct
    11881 aggtacttac attcttaaca ctgtgactac ccaagccttc ggtgaactcg gacgtagtgt
    11941 cttaggagtg attgtttcac ttgcctgttt aaccactgct gtcggtttag ccgtatcagt
    12001 cagtgaatac tttaatgaaa ttttcccgaa aatttcatat aaagtttatg ctatcttatt
    12061 tactgtgatt agttttactg ttgctaacca aggattaaaa gaagttatta gcaaatccat
    12121 tccggtatta ttagttctct acccaatagc aatgactgtc attttaatgt tgctgattaa
    12181 tttagtgatt aaacttccgt tgtctgcaca acgtctatca ctcctattag tgacaattgt
    12241 agcgatagct tcagttgccg gtgtcgggtt taccgaaaac ttgcctctaa aatcctactc
    12301 gatggaatgg ctacctttcg ctatcattgg agctattatt ggagcaatta tccacccgct
    12361 taccggtcga aagcataaat aaagtaaagg cagtgagcat tcactgcctc ttttttagga
    12421 gagaataatg cctgaattac ccgaagttga aaccagcgtt cgtggtgtat cgccctattt
    12481 ggttgggcaa actattgaac aaatcatcgt tcgtcagcca aagttacgtt ggacggtgag
    12541 tcctgaatta agccaaatgg caggggcaaa aattaccgca gtagaaagac gtgccaaata
    12601 tctgattatc cgcaccgaca aaggcgatat tttggtgcat ctgggaatgt ccggctcgct
    12661 cgggattttg caagaacgtc aacaaaaaga aattggcaaa cacgaccacg tcgatcttat
    12721 tacccaaaac ggcacaattt tacgctataa cgaccctcgc aaattcggct gttggctatg
    12781 ggcagaaaaa gcagaagatc acgaactgtt gcgtaaactc gggcctgaac cgctttcaaa
    12841 cgatttcaca gcagattact tatttgcaaa aagccgcaaa aaaacgaccg cttgtaaaac
    12901 ctttattatg accaatgaag tggttgttgg ggtggggaat atctatgcgt gcgaaagttt
    12961 atttatggca ggcattcacc ctgagcttgc agcacaaaac ctgactaaaa aacagtgcga
    13021 acgtttagta aaaaccatca aagaagtgct gactaaagcg attattcaag gcggcacaac
    13081 actgaaagat tttatccaac ctgacggcaa accaggctat tttgctcaag tgttgcaagt
    13141 gtatggaaga aaaggggaaa cctgtaatga ctgcggaaag ccgattgaaa ctaaagtaat
    13201 cgggcaacgt aatacctatt tttgcccgaa gtgtcagaga ttgccgaagt aatctaataa
    13261 taaactctct ccccctgagg gagagagaca gcttgttgaa acgttaagtg aaacaagcag
    13321 agagagggta aaacctaaac ctgcaaccaa aaggtaacag gcccgtcatt ttgtaggctg
    13381 acttgcatat cggctgcaaa ttgcccggtt tgggtatgaa tcttagtggc ggcttgttga
    13441 tgaaagtagg catacaaacg ttcggcatca accgggcttg ctccttttga gaaactagga
    13501 cgcaagcctt tattggtatc cgccgcaagg gtaaactgtg aaaccactaa caaactgcca
    13561 cctgcttgct gtacgtttaa gttcattttc ccctcgctat ccgaaaacac ccgataattc
    13621 agcacttttt ccagtagctt atctgctttc gcttcatcat cgtctttttc cacaccgagc
    13681 agcaccaaca aaccgcttgc aatttcgcca acgacttgcc cttcaacctc cacttttgcg
    13741 aatttaaccc gctgaattaa gcctatcatt aatgctcccc ttgtctgttt ccatttcgtc
    13801 taaaaccgcc gcaaattgcc cgccaagtaa taccacctgc caacttaact gaatccataa
    13861 cagcataatc ggtaacaccg ctaatgctcc ataaattgcc tgataagacg ggaaggcagt
    13921 aatataccaa ataaaggctt tcttccctaa agtaaagaaa atcgctgcca ttaatgcccc
    13981 gatagcagcg tgttgaaatt ttacctttct aattggaata aatttataca ctaaggtaaa
    14041 aagtagccaa attagcaaaa acggcatcaa ttctaataaa tgctgcccga gcgataacgc
    14101 tccatttcca ttaaaaatag aaagcgacat tacataggtg ctaatcgcaa tgctagcacc
    14161 ggcaagcagc gggccaaaca gcaaaatgag aaagtaaagc aagaaagaga accacatcgg
    14221 acgctgacga ttatcgtgcc aaatggtatt caatgcctca tcaatactct taatcagcat
    14281 aatagtaacc acaaataagc cgataaaact taccagcccc atttgtttgg cattactgac
    14341 aaacaactca atgtattccg acaccatctc gctcgcactc ggggcaaagt tgtcataaat
    14401 gaacgctttt aactgctcgt ttgcttcgtg gaaaatcgga aaaaaggtaa aaatggaaaa
    14461 cagcacgaca ataaacggca ctatcgccag cattgtgcta taagtgagat aaccggctga
    14521 aatcgcaatg ttattttcat tgtaacgctt ggcaaagagc ttaataaatc tccacagtcg
    14581 atttaacatc ttaacctctc accaaataat cgccttcaca cacccatttg taggtggtta
    14641 aggcttctaa tcccatcggg cctcgtgcgt gtaatttctg cgtgctaact gccacttctg
    14701 cccctaaacc aaactcacca ccatcggtaa aacgggtgct ggcattaata taaaccgccg
    14761 cggcatcaac ctgttgtaca aattgacggg caagttgata atcggaggtt aaaatcccct
    14821 ctgaatgctg cgaaccataa tggcggatat gctctaccgc ttgttgcagg tcattcacca
    14881 ccaccacgtt aagatcaagc gataaccact cttggctcaa ttttgcttga tcaagcggtt
    14941 caattccttc aattttttgc aaatcgttac tgtgaatagt aacgccaagc tgttgcattt
    15001 tggcgaccaa tttcggcaaa aattccaccg caatcgcttt ttccaccaac agcgtttcca
    15061 gcgtattgca agtgcttggg cgttgcgttt tggcattagc gatgacctct agagatttct
    15121 cctgatccgc tgttttttcc acaaacagat gacaaacccc aatccccccc acaatcaccg
    15181 gaatggtcga attttcttta cagaattgat gtaaacctgc accgcctcga gggatcacca
    15241 tatcaatgta gcgatctagc ttcaatagtt ccaataataa ggctcgatcc ggatccgtca
    15301 ccgcttgcac tgccaatttc ggcaagccgg ctttttctaa cgcatcttgc actacatcaa
    15361 ccaacacagc attggtaaat ttggtctctt taccgccacg caaaatcact gcattgccgg
    15421 tttttaggca taatgatgcc acatcaatgg tcacattcgg gcgagcttcg taaatggtta
    15481 aaatcacccc gagcggcaca cgctggcgtt cgattttcaa accgctgtct aacacgccac
    15541 cgtccatcac ctgccccacc ggatccgcta atttggcaac atttcgcaca tcgttagcaa
    15601 tgtcttttag ccgatcttca ttcaataata agcggtcaat aatcgccaat gaaatccctt
    15661 gtgcttgagc aaattcaata tctttggcat tagcgtataa aatttcttct gcacgttgct
    15721 caaggctatc ggcaatcgtg agtaaggctt ggtttttctg taggttgcca aattgagcca
    15781 gctcaaaact agcttgtttg gcttgctggg caagttctat catctgcata aattaggtct
    15841 catttaatca atattctcca taatctaaca cgttctttta tgtgaagaaa tatgcaaaag
    15901 ccaagaaaat tggataaaat gggggaattt taaaacatta cgcaattccc ctctccctga
    15961 tttttctcct gaacggagaa aaatctatcc ctctcctaca aggggagagg gtagaattta
    16021 actctctccc cttgtgggag agagaccgcc tacagattcg ttcagaatct gtaggcagag
    16081 agaggggtaa aaacagataa aacttaaaca aaatgataaa cactacccct aaacagctaa
    16141 tccacattct tccgcctcaa ctggcaaacc aaattgctgc cggcgaggtg gtggaacgcc
    16201 ctgcgtctgt ggtaaaggag ctagtggaaa acagtttaga tgccggagcc aatactattc
    16261 aaatcgacat tgaaaaaggc ggctctcagc ttatccgcat tcgagataac ggttgtggta
    16321 tcagcaagca agatttaccg ctggcacttg ctcgccacgc tacgagcaaa atcagcagct
    16381 tggaagattt agaagccatt ttaagtttag gctttcgggg cgaagcattg gcaagtatca
    16441 gctcggtttc acgtttaaca ctgacctctc gtaccgcaga gcaaaccgaa gcatggcaag
    16501 cctatgcaca agggcgggaa atggcggtgg aaattcagcc tgcctcacac ccgattggca
    16561 caaccattga agtagcaaat ctgtttttta acacaccggc acggcgtaaa tttctacgca
    16621 ccgataaaac cgaatttgcc catattgacg aggtggttcg ccgcattgcc cttgccaagc
    16681 cgaatatcag tttcagctta agccataatg gtaaaatcgt ccgccaatat cgcaaagtgc
    16741 aagacaattc ggtagagcag caacaaaaac gggttgcagc catttgcggt gatgagttta
    16801 ttcaacattc aacttatatt gacaggcaac acggggatct gcacctacac ggctgggttg
    16861 gctcaccggc tcttgcccgt ctgcaaaacg acctttgcta cagctatgtg aacgggcgaa
    16921 tgatgcggga taaaaccatc aatcacgcca ttcgccaagc ctacggcgac agcattgcaa
    16981 gtggttacta tccggctttt gtgatctttt tggatttaga tccgacaatg gttgatgtaa
    17041 acgttcaccc ggctaaacac gaggtgcgct ttcatcaagg aagactggta cacgacttta
    17101 ttttgcaagg cgtactcaac agcctgcaac aaacccaacc tacgcttgaa ctgcgaaatg
    17161 aagtgaacga gccgttaccg agctatgttc aagacactaa ccgccaagct gcggggcaaa
    17221 atatttttgt gtcgcaaacc gctccacaag cggccgaatt tacgcaaaaa aatgcaagtc
    17281 gatcaagcta tacgcctaaa tttgaacgaa gcaacagtgt gagttcgtcc gctcaaaaat
    17341 ggtataagga attggttggt ggcgatttgc accaaacaac gccaactatc aaaacgcaaa
    17401 gcacgcttgt tgagccaaaa acagaacccc aaactgaaca taaaacagaa gccgtttcgc
    17461 aaaaaattac gcaaattgca ccgcttgcaa gccaccattc acaagcctta gcgattgtaa
    17521 aaaaccaagc ggtgttaatt aaagaagagg aaaattttta cctgcttcca ctggcaacat
    17581 tagccgagct taaactcaag ctacaactgg aaaaagggca atctcaagcg ttgttgatcc
    17641 cactgaatct cagtttagat caacaacaaa ccgaacgctg gcaacaaacc aaagaagaac
    17701 taactaagct tggcttttcg atcagcgaaa aacagtggca agcccaaaca cggctcacag
    17761 tgctttccgt accacaggct gtcagagaac aaaacctgca acagctttta tttgctctgt
    17821 tcaaccaaac acaagcggtc aatttagccg aattttttgc aaaaaaatgc gaaaaaccca
    17881 ccgcttgtac gctaagtgac gtgattgctc tactctcaga aattgagctt tatgccaatg
    17941 ggaaaaaaga gctggaggga ataaggaagg aagtggattt ttccaagttt ttatagtcta
    18001 aagggcaata ccctttagcc aaataaatga gagtggctgc caattcgcag cagctctagc
    18061 ttatcatctt ccacttttcg atagataaga accaaatcag gttggatatg gcaatcccta
    18121 aagcctttcc attcgccggt taaagcgtga tcgcagtatt tctcaggtaa tggcttgtca
    18181 ttaatcagaa aatacaacgc ctcaattaat gaagaggata aagactcaac cgcacatttt
    18241 ttatagtcac gtttaaaagc attactatac tcaatctctc gcattgagtt gctccattaa
    18301 atccgcaata ctatttgctg aatgtagctt ttcctcgcca ttcttaacag cccttaatac
    18361 ttctgctgtc tcaaaattag gctgataatc aaactgaaaa cgtttttctg ttgcgatttt
    18421 cgctaatgtc attcgcatca catcagagac agtcaagccg ataccggcta acacctcact
    18481 tgcttgctct ttaatttcag ggctgattct tacgcttact aatgcactat ttgccatcaa
    18541 aaactccttt tgtatatcaa atgatataca aaataacatt taacaaaaaa ttgtgcaata
    18601 aaaaagaaat tcaaagaaaa gaaatgaata gttaaaatta gctcgttaca agaaatcatt
    18661 gagttactca agaaatcaag gtaatataaa gaagtctggg cggaacacga acccgcccct
    18721 agcatctagc cgcttacaag aacagataga ttagttgaaa gagcgtgaaa atttcaactg
    18781 ctgcacacag aaacccaagt gcaactaaaa tcaatagtag ttctttcatt gtaagcctcc
    18841 ttagtgctac taaggatgat ttaggctcag gctactgcga atagccctaa cgagcctacc
    18901 agtagcacaa tcaggattgt aacaaaaaag ctgtgaattt cacggctttt tttgtatcta
    18961 tatacagtat attaatatga ataaaaaacc ccttgccctt tttttaatgg ggccgactgc
    19021 ctccgggaaa accgatttag ctattgcttt acgccaagaa ttgccggttg aggtaatcag
    19081 tgttgattct gccttaattt acaaaggaat ggatattggc acggcaaagc caagcaagga
    19141 agagcttgcc cttgcaccgc atcgcttgat tgatatttta gaccctgccg agagctattc
    19201 ggcgatgaat ttccacgctg atgcgttgcg tgaaatggca gatattacct cacagggtaa
    19261 aattccgctc ttggtgggtg gcacaatgct ttattacaag gcattgcttg aagggttatc
    19321 tcccctgccc tctgccgatc cagccattcg ggctgaaatc gaaagccgag cggcacaaca
    19381 cggctgggca acattgcatc aagaattagt gaaaatcgac ccgattgccg gtgcaagaat
    19441 taacccaaat gacagccaac ggatcaaccg agcgttagaa gttttttaca ttaccggcaa
    19501 aacgatgacc gaactcactg ctcagcaagg tgaggctcta ccttataata tcttacaatt
    19561 tgcaatcgct cctcaagatc gtgcagttct acaccaacgg attgaacaac gctttcacaa
    19621 aatgattgag ctaggctttg aagaggaggt caagcggtta tttttacgca aagatttgca
    19681 tattaatttg ccctcgattc gctgtgtggg ctaccgccaa atgtgggaat atttgcaagg
    19741 cgatatttcg ctcgatgagg cgatctttaa aggcatttgt gccacccgcc agctggcaaa
    19801 acggcagatt acttggctgc gtggctggca aggcgaactg acttggttag atagtttagc
    19861 tccgaccagt tctaaagcaa aaatggtgga aaagatcgat cattatttaa agaattgtga
    19921 taatatctaa acgcctttgg cacttatcgc attatttggt gtcttaaaaa tcacacaact
    19981 ataaaaagga aaataacaat ggcaaaaggt caatctttac aagatccata tttgaatgca
    20041 cttcgtcgtg agcgtattcc tgtttcaatt tatttagtta acggcattaa attacaaggt
    20101 cagatcgagt cgttcgacca atttgtgatt ttattaaaaa acactgtaag ccaaatggtg
    20161 tataaacacg ctatttcaac ggttgttcct gcccgtgcgt tatcgcataa caataacaac
    20221 caagcccaac acgctcagcc tgctgccgaa gtaactgaac aagcggaata ataacattat
    20281 tactcaacca atgttaaacg aatcattgcc aaactcagaa gaaaattctg cgtttggctt
    20341 tgctttatct gaagcaagcc aaactacaga agccgcctcg cctaccgatc cagaaaatca
    20401 agataaagcc attttggtgc atctctatct cgggcaacat aaagatgttg aaaatttgct
    20461 ggaatttaaa acgctggcag aatctgccgg tgtcgaagtg ctggcaacgc ttaccaccag
    20521 ccgccaaagt tcgcatatca aatattatgt ggggcaaggc aaagcggagg gaattgccga
    20581 agcggtagaa cgactcgggg caacagtagt gctggtgaac catcaactta gcccggctca
    20641 gacccgtaat ttgcaatcgt tatgcggctg ccgtgtggtg gatagaaccg gcttgatttt
    20701 ggatattttc gctcaacggg caagatcgca cgaaggcaaa ctacaagtcg agctggcaca
    20761 acttcgccat ttatcgaccc gtttggttcg ccgagttact aatcaagacc aacaaaaagg
    20821 cggagcagtt ggcttgcgtg gtccaggtga aacacagctc gaaaccgatc gccgtttgat
    20881 taaagtgcga atccaacagt tactcaaccg cttggaaaaa gtcagcaagc aacgcaatca
    20941 aaatcgaaaa accagacaaa aagccgatat tccaacagta tccttagtgg gctataccaa
    21001 tgccggcaaa tctaccctgt ttaatgcgat tacccaagct ggtgtttatg ctgccgatca
    21061 actttttgcg acccttgacc cgactttacg ccggattcag gtgcaagatg tcggcacgac
    21121 aattcttgcc gatacggtag gttttatccg ttttctaccg cacgatttag tttcagcctt
    21181 taaatctacc ctgcaagaaa ccaccgaagc tactttactg ctccacgtca ttgacggctc
    21241 agacgaacgc aaaaacgaaa atattgatgc ggtaaatcaa gtgttggacg aaatcggagc
    21301 gttagatatt cctactttat tggtattcaa taaaattgat aaattagaag gtgtagaacc
    21361 gcatattgaa cgcaatgccg atggcacgcc agttgccgtg tatctttccg cccaagaaaa
    21421 tagcggcatt gagctgttat tccaagccat tcaagaaagg cttcgcagta acttagtgtg
    21481 tgaaaacttg ctcctcccgg ttactgccgg tgcaatttac gcacaattta aagcagaaaa
    21541 ctgcattaaa accgagcatt ttaatgagtt cggtgaacgg attatctcta ttgaaattaa
    21601 cgaagtgcaa tggaacaaat gggtgaaaca atttccggat ctagccgaat atattgagct
    21661 ggcaaaatgg gggtaattaa tctcacttct gagtaagtta tatttaaatt atgcaagcgg
    21721 tcaattcttt acgttttttt gcaaaaaagt gtaaagaatt gaccgcttgt tttatccatt
    21781 aaatccaatt tagccaataa aaaaatccct caaataaacc agatttcccc gttctatttg
    21841 aaggatttta tttattcttc cccaaaagtg gggaagaaaa gcttaatcaa gcagttacta
    21901 ccactgataa cctacgcttg cagcagcacc tgtatcacca cggctgttag agttaacact
    21961 gaatttgata cccactttac cattatctga taaacgagaa taacctacag caatagctcc
    22021 ttgaccacgg taagcaccta caccggctga aaccatcgat ttaccaggtt gtgttacgtg
    22081 atataaatta cctgcggcta atgcacctgc aatacctgca cgtaagtctt tatctacgtt
    22141 actgatacga ttgttcagac gattttctgc acctttcaac tgattcacat taacagcatc
    22201 agtgcctttc acacccggag caacgttcgt aatacgtgtt tgcgcattct tgctacctgc
    22261 aatgttaagc tcatttacgc catcttttgc aacagttgaa ctgatagttg taccaccaac
    22321 tttcactgaa tcaaattcaa gattgcttga ggttgccact tcaacagttg aacctttacg
    22381 tgaaacaacg atgtttttgc cacctgaaac ggccacttta ccattgttat taacacgttg
    22441 agcacctttc tctgaagttt cagctacatt accatcaaca gtgagatacc agcctgtatt
    22501 ctgcattgca ttatacaact ggctaccgct cacaacatct ttacttgttg ccgttacatc
    22561 accatcttta acgttagtga tcttcgtgcc tttcgcatca ataccgcctt tagttacact
    22621 tgggccgtct ttaatggtta aaccattgtt atttacggtt gtgtcgccag ttgttacaga
    22681 accatcttcc gttaaatcaa ttgctttcgc taattgtacc tctaatgtgt ctgtaccatt
    22741 cgctttcaca ttgatgttgc ctgatgctga cttaaagttt tcagaagctg ttttatccac
    22801 accttcacct ttcactttaa cttcagtatt cagtttctga cggttcttca catcttcatt
    22861 gttaccagag aagattaaac catcatttaa ggttgcaact tgttctgtga cagttttacc
    22921 gtctttatct ttggtctcat aaacgatacg agtcttaccg ttctcacccg gtaatccatc
    22981 tttaccattt gtaccatcaa caccttcagc accatctttc actgcaatat ttgcacttgc
    23041 accatcttta ccgtcagcgc ctttcggacc ggttaagccg atagagccat ctttaccgtt
    23101 taatacaaca gaagcgccat ctttacctgt agctccaatt gaaccatcaa caccgtcttt
    23161 accgtcacgg cctgcagtaa ttgtgccgtc acctttaatg gttacgccat ctttaccgtc
    23221 tttgccagca ataccgattt gaccgtcttt accatcttta ccaccaatgg ttaagttgtt
    23281 tttcaagccg aaggtcacgt tattaccttc tttagtaata ataatgttac cgtctttgtt
    23341 gtttagatca actgttgctc ctggtttaac gttgcctttg tctttaccat ttgctgttaa
    23401 gttccaacct gcgttagcaa cttcttgcac ttgatataac tggctaccat ttaccgcatc
    23461 tttgctggtt gcagaaattt caccgtcttg aacgttaatg attttcttat cgcctgcatt
    23521 gataccggct ttagttacac ttgggccatc tttgatagtt aaaccatcat tatttaccgt
    23581 agtatcacca atagttacag aaccatcttt cgttaaatca atgtctttcg ctaatttaac
    23641 tgttaacgca ccattgttgt ttacaacacc gatattgtta tcggacaggt tcgttaaatc
    23701 agtatcaccg cctgagattt ttaacgtctc attaagtttc ttaacgattt ctttaccgtc
    23761 atcacctacg aatcttaagc catcgttcag tgttgcaact tcctcagttg taccgtctgg
    23821 tttctcataa acgatacggg ttttgctttc gccatcttta ccgtcgttac cgtcaaggcc
    23881 tttagcaccg tcttttacag agatagttgc acttgcacca tctttaccgt cagcgccttt
    23941 cggaccggtt aagccgatag agccatcttt accgtttaaa gtaacaccaa ctgtaccatc
    24001 tgcaccttta acaccgattg aaccatcttt acctggaccg ccaatggtaa aatcgttatt
    24061 taacgtaaag tcaatcacat tatcacttac tgttgtgatg atgtttttgt ctttagaggt
    24121 gtacttaaca gtttcaccta gtttaacgtg atcagtttca ccattatccg ctgcaatgct
    24181 aaagcctttc tcgataatat cgttgcttgc atttacaaca tcattaatat tcgcagcgtt
    24241 agtaccaact tcttttaatt gatcgtctgt taagtcgttc actgaagtga ttggcttacc
    24301 atctttaccg ttaatgccgt ctttacctgc taaaccgtca ataccgctct tcacgttaac
    24361 aatttggttg ccgccattgt ctagaccgtc ttcagtaagc ttaactgctt taccatctgt
    24421 gccatctttt ggcgtgatag ttacgccatt attatttact actgttgttg gacctttacc
    24481 ttcttgacca ttctctccat cttgtggttt gaatgtagct gagttcaagt ctttaagatc
    24541 tttagctaat tgaacagata atttaccatc tttgctgttt acgccgatgt tatttgcagt
    24601 tagcttatct ttatcagctc caccaatgat ttctaaggtt tcattcaatt tcttagatac
    24661 tagtgttgca tcatcacctt taaagtgtaa accatcattt aaagtagcta cttgctctgg
    24721 ttcacctata ggatcaccat tgccatctgt tggctgatat tggatacgag ttacaccatc
    24781 tttacctggt aaaccatctt taccaacaag ccctggtttg ccctcagtac ctttaatggt
    24841 taaaccatct ttaccatttg taccgttcaa gccgattgaa ccatctttac cgttaagaac
    24901 aactgcagaa ccgtctttac cgtttacacc gatagtacca tcaataccat ctttgccagg
    24961 gcgacctaca gtgatagctc catctttacc atctaaagca atagcatctt taccgtcttt
    25021 accagcaata ccaactttac ctggttcacc atctttacca acagtaatat tattcactag
    25081 gctaacttct aatgcatggc caccatcttt ctcaacaacg tttgttttaa cattgccatc
    25141 accaatgatc ttagctgtcg tacctaagtt ttgcttaaac tccttgttgt tctcatcttt
    25201 tagaccaaac gcatctgttg tcacctcagt aacagtctca gtaacagtat ttttcaagtc
    25261 accgacgtta accgcattgt taagaacatc acctgctgcc tcagtaagtt taacaggttg
    25321 accatcttta ccttttaagc cgctatcaac attggtgatt tggttaccac cgttgcttaa
    25381 gccatcctct gttaaggtaa cattagattt agttgcatca ttaggatctt tcttagcaat
    25441 cgttacacca tctttattga taactgtaga atcaccttgt gtgccgaatg ttgcactatc
    25501 taagcctgct agatcttttg ctaacgccac agtaatggta ttgccaacaa cctgtgtcat
    25561 gatattttta ccagcatcaa acttagccca atcagtgttt gttgtagcac ctttaatatc
    25621 cacctgcgta tttaatttcg catcgtgaac tttgccatca ttcgcaccga attttaagcc
    25681 atcgttaagt gttgcaactt cttctttatc accatttggt ttttcgtaaa caatacgagt
    25741 tttgctttcg ccgtctttac cgtcgttgcc gtcaagacct ttagcacctt tctcaacttt
    25801 aattgatgct ttaccatctt ttccatcttt tccatttacg ccatcaacgc cgtggaatac
    25861 gatggcttct ggtttaattg ttacaccatc tttaccatca gcacctttaa caccaactga
    25921 accatcttta cctggtttac caccaacaga gatgttatcg gataagccaa aatcgatggt
    25981 attatttgtt acggttgtaa caacattctt atcggttgag gtgtacttaa cagtttcacc
    26041 taacttcaca ttgtcttctt tagcaccacc tcctaagcca gagtgatccg ctgtaatgtt
    26101 aaagccagtt ttaactaaag tgtcagctac tgcgtaaagt tgtgaaccgt taatcgcatc
    26161 tgttgaattg gaagcaattt tacccggtgc aacaaactta atttggcgag cattggttgc
    26221 attacccaca ctcacataat caccatcaac aacattttca tcgccagcaa agctgctata
    26281 ttttaatgtg ccaacagttg cagtatcttc tttagttgcg gttgcttcag tagagtcaga
    26341 acctagaatt acagaaccct ttgtcttaac agtaacatta gaacctaata cgaaagtatc
    26401 tttacctgaa actgtattgt tataacctaa agccgcagca cgaatacccg aaacagtact
    26461 atttacaccg gctgcaattg agtttttacc ctttgcggtt gagttgtaag caatagcagt
    26521 tgcataatta ccttttgatt tggcctcaaa accaattgct gtgccacctt catgcgtaat
    26581 attggcacgt gtacctactg ctgtcgcatt cgccttactt acattagcac tataacctat
    26641 cgcaacagaa ttattaccac ttgaattagc cgtatcacca attgccattg caccatcttt
    26701 gctcgcgtta gaatgcgaac caatactgat cactttttct gcatgagagt ttgcattcca
    26761 accaatgcta atagcattac ttttccaggc tttagaagca ttaccaaaag caatcgcacc
    26821 gattcctgtt gtattcgaat ttacaccaaa ggcgatagaa ccatcacctg ttgcacttgc
    26881 atttgttgaa aacgccaaag tatcattcga atttgcacga gtattcgtac ccaaagcaaa
    26941 tgcattattt gctactttcg ctgtatcagg attaattacc tgtcctaatt cagaaatatt
    27001 tgaattctct aatttaaagc caccggacca agcgttagta ccaatggcaa atgaatggtt
    27061 accattagtt ttcgctttag taccgattga gaatgcatct gttccaaatg ctaaagtatt
    27121 attttgccca tcataaattg aacgtctatt ttcatgaccg taacttccat ccactgtctg
    27181 tggagtacca ctaccaatcg caaaagaacg gtcacctagc gctgtagccg aagcaccaat
    27241 ggcgaagcta gctgtagcat ttgcccaaga tgttgaacca attgcagtag aataatctgc
    27301 aattgcagca gattgacgca ttgcagcaaa tgagttaaat ccttccgcac gtgcagaggt
    27361 acctaatgct acagaaccaa tacttgcagt acgagaaaaa gaaccaattg caatagacaa
    27421 tccccctcca gaatatgaat aatcaccaat ggctatgcct gacgctttcg gttttcctct
    27481 actttttctg ttattgtcta aagctacagc attgtgtcca attacaattg ctgaggtagt
    27541 attagactta tacaataatc tagaatcctt ctccttatta acagagttat tagattcttg
    27601 aagcttagtt ctgtctgcat aggataagct acctgggtta aagtagttat atggattcgt
    27661 aatagaatct gtagccaaac tagtgtttgt ataaatatct ataccaccat taacaacttt
    27721 ttttggtgct ttttgttgta cttctattct atcaataatc accgccgctt ctgccggtgt
    27781 agcagaaagc ataaaggctg ccattccact gataagggtt gctcccccta gaatgctagc
    27841 cgctgctaat ttaatagtac cgcgcttatc agtagaagaa gcggctttac cttgcgatcg
    27901 agcaagttca gacacaactt ccattttttg cgtcgtttgg ttaaaaataa ccttaaatat
    27961 tttgttcata attttatacc tttagaatta ttataaagct tacttcgtaa ataattcggc
    28021 aattacaacc caccgaataa acaaccttac tactcttaat ttagtaagag gaaagtcata
    28081 tcgataatag taagattttc acttcaaaag tgaaacaagc ttttcacttc tttacaattc
    28141 tcttattatc aagataataa aattaacaaa accccttata aatcaaacaa atacacagta
    28201 aatatactta atttacaaat aaataccaaa
//
LOCUS       DANLRK010000011        28128 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.011, whole genome shotgun sequence.
ACCESSION   DANLRK010000011 DANLRK010000000
VERSION     DANLRK010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 28128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28128
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.011"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(10..630)
                     /locus_tag="PXA33_000331"
     CDS             complement(10..630)
                     /locus_tag="PXA33_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HDL5119705.1"
                     /translation="MCRFPLFETLSIIDGKVQNLQYHQQRFEQATQEYFGCEPRFSLA
                     DILVVPEASQRGKVRCRIDYNATEFELKFFIYSPKKVERFRLVEVENWDYHLKFSDRK
                     RFDLLPILQNEEVIILNNGYVSDCSIGNLLFLKQGIWYSPQHYLLKGTQLSKLLDEGK
                     VVLAKIAKQDLHQYEKIMLINALNPFEEPRALPCSQIYFCDLDRKS"
     gene            complement(608..1582)
                     /locus_tag="PXA33_000332"
     CDS             complement(608..1582)
                     /locus_tag="PXA33_000332"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005612571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HDL5119706.1"
                     /translation="MQDFIHQANHYGQAKRPFFFLIDFEQQKPLIFPLEQAHASGLFF
                     EFFCKSNLEKPVEKPTKAFELSANPIPFSEYQTGFELVKKEIQAGNSYLLNLSYPTAI
                     ETNYSLAEIFAASKAKYKCYLQGRFVCFSPECFVRIENNRIFSYPMKGTINANEENAE
                     QKLMASEKEFTEHNTIVDLIRNDLALVAKNIEVTKYRYVEKVETHRGAILQTSSEICG
                     ELGENWQENIGSILAKLLPAGSISGAPKEKTVSIIQQAELGKRGYYTGIFGYFDGESL
                     ESAVAIRYIAQTSNGLVFHSGGGITSQSVLTDEYNELLEKVYVPISAI"
     gene            1692..3299
                     /gene="rmuC"
                     /locus_tag="PXA33_000333"
     CDS             1692..3299
                     /gene="rmuC"
                     /locus_tag="PXA33_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HDL5119707.1"
                     /translation="MHFQEIGIYIALAVCVLVGVYLFFLKSRYQRNAYELSQDLGKIT
                     ENFEQIQQKYEVLAQEKNQLEQWAIQQQTKYEAVSERLNERDGQLQRFQQKIELAEQQ
                     ENQLERYINELKERVGSSQAKAESLEEQLQFSQSSLSTKERENQALFSRLNEVQNELT
                     ELRTTLSEKQANFEAQQRNFIEVKQQLNVEFQHLAQQILDEKSKRFSETNQSSLEALL
                     KPFKEQIEGFQKRVNEVHSESLKGSANLEAEIKRVLQIGVSMSEEAQNLATALKGNNK
                     IAGNWGEVQLESALQSAGLLAGEHYQAQESFRDEEGRRFAPDFVVHLPDQKHLIIDSK
                     VSLVAYDQAVRSEENFAISQALDEHCRSLRNHIEGLSKKNYSALLGIKSPDFVLMFIP
                     IEPAYIEAMKHDPQLFNFGYERNVILVSHTTLMPILRTVANLWRIERGNSEAREISEK
                     AGEIYNQVCAVADRLAKLGNTLNTANNQYNQTVTSLVGRQGLLGKVERFQHLSSKASQ
                     IVPQVELIENEVDTIKLEMVSRELLEE"
     gene            3362..3946
                     /locus_tag="PXA33_000334"
     CDS             3362..3946
                     /locus_tag="PXA33_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component II"
                     /protein_id="HDL5119708.1"
                     /translation="MLKKLLIIDNHDSFTFNLVDLLRKLDVPAKVVLVEELDLNEVEH
                     FSHILISPGPDVPRAYLQTFAMLERYHQTKSILGVCLGHQTICEFFGGKLYNLNDVRH
                     GQQRTLTQIEPNPIFKGLPKQLKVGLYHSWGILQNSLQNTPLVPTAVCDQNVLMAFKH
                     QHLPIFGVQFHPESFITEFGQQMLANWLEITQDF"
     gene            4043..5125
                     /gene="prfA"
                     /locus_tag="PXA33_000335"
     CDS             4043..5125
                     /gene="prfA"
                     /locus_tag="PXA33_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005713004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HDL5119709.1"
                     /translation="MKDSIINKLESLSERHEELQALLGDASIISDQDKFRAYSKEYSQ
                     LEEVVGTFNRWKKLNNDIEEAQLLLDDPDMKDMAAEEIAENKAEIENLEQHLQILLLP
                     KDPNDEYNAFLEIRAGTGGDEAGIFAGDLYRMYSRYCESKRWRIEEMSANESEQGGYK
                     EIIVKISGEGVYGQLKFESGGHRVQRVPKTESQGRIHTSACTVAVMPELPESEMPEIN
                     PSDLRIDTYRSSGAGGQHVNTTDSAVRITHIPTGIVVECQDERSQHKNKAKALAVLAS
                     RIVQVEQERQAAEQADTRRNLLGSGDRSDKIRTYNYPQGRVTDHRINLTVYRLDEVMN
                     GKIDELIQPIITEYQADQLAALSEQN"
     gene            5237..5839
                     /locus_tag="PXA33_000336"
     CDS             5237..5839
                     /locus_tag="PXA33_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5119710.1"
                     /translation="MRKLGLLAVFTLVLSACSNSTKIEQTILSKQDIVGSWVCAIKYD
                     DLKVSTLDFSEFKANGEMTNFGDISDENFEPIKFTYVTGDKGKWDLKGNRLVIDYDLS
                     ERKVKRTTPKKFLEFLKQEERKASKTAKKLLKYEQGIFNILSDQKNTENSKIILDILK
                     FGHSRMAIQQNMGDKVYSGGCVTADKAEEYLDILKNRNSK"
     gene            5839..6705
                     /gene="prmC"
                     /locus_tag="PXA33_000337"
     CDS             5839..6705
                     /gene="prmC"
                     /locus_tag="PXA33_000337"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDL5119711.1"
                     /translation="MDYREWLTFAEQKLLENSGADPYLNAKFDANLLLQAVTKRSKSA
                     IFAFSETLLSESELAELAEKLARRAKGEPMAYILGYREFWSLPLKVSPATLIPRPDTE
                     RLVELALEYANKRLGNQKMLQILDLGTGTGAIALALASELGEKAQIIGVDFQNEAVQL
                     AEENRRNLGLNQVTFLQSDWFSSLANQQFDLIVSNPPYIDESDENLTVGDVRFEPLTA
                     LVADNKGLSDLQKIIENAPLHLKPQGALMLEHGWKQGNLVRDLFDLHLWEQVETAQDY
                     AGNDRVTKAILK"
     gene            6714..7580
                     /locus_tag="PXA33_000338"
     CDS             6714..7580
                     /locus_tag="PXA33_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB1 family protein"
                     /protein_id="HDL5119712.1"
                     /translation="MDDIIEELLKELEDPKVTISETELKKFLYREILRLTTLIDKNVS
                     EQQVFGQMSALVKKARYNVNGEGDEERINQLLHLVYREWGFHCYYEDYFHTENLLLNQ
                     VIRKRRGMPVSLGAVALYLSAVLDLPLYPVNFPTQLVLRAEIHQPNGATKVRFINPWN
                     GEFLTIEMLNKWLEGEMGFGVEVTPDLLRRAEPQELLERVETVFKMALTREGKYEETL
                     RLIEYRLAFSPEDPYEIRDRGMVLASMDCYQAALEDINYFIDQCPEDPSAEILKMEVK
                     GLQQKSQESVVH"
     gene            7670..8524
                     /gene="kdsA"
                     /locus_tag="PXA33_000339"
     CDS             7670..8524
                     /gene="kdsA"
                     /locus_tag="PXA33_000339"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012073169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HDL5119713.1"
                     /translation="MTDKIVKVGNIEVANHKPFTLFGGMNVLESRDMALRVCEQYVEV
                     TQKLNVPYVFKASFDKANRSSIHSYRGPGMEEGLKIFQELKQTFGVNIITDVHEIYQC
                     KPVAEVVDVIQLPAFLARQTDLVEAMARTGAVINVKKPQFLSPGQMGNIVEKIAECGN
                     DQVILCDRGTNFGYDNLVVDMLGFGIMKKVSKGCPVIFDVTHSLQCRDPFGAASGGRR
                     DQVTELARSGMAIGLAGLFLEAHPDPNSAKCDGPSALPLSKLEAFVSQMKAIDELVKS
                     FEEIDTSK"
     gene            8745..9692
                     /locus_tag="PXA33_000340"
     CDS             8745..9692
                     /locus_tag="PXA33_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HDL5119714.1"
                     /translation="MLNIVFLDRTGIPATHDIPRPNFPHTWTEYATTSADQTFERVKE
                     ADIIITSKVLLQRDLLAKLPKLKLIAITATGTNNVDLAAAKELGIAVKNVTGYSSVTV
                     PEHVIGMIYSLKHRLADYHRDLITSDRWATCGQFCYVDYPIHDVRGSTLGIFGKGNIG
                     AEVARLAQAVGMQVIFAEHQGATEIREGYTAFEEVFKQADIISLHCPLTEQTQNLINA
                     ETLALMKPTAYIINTGRGPLIDEQALLEALEQGKIAGAALDVLVKEPPEKDNPLMLAA
                     KRLPNLLITPHVAWASDSAVTTLVNKVTQNMEEFVATGM"
     gene            9790..10962
                     /locus_tag="PXA33_000341"
     CDS             9790..10962
                     /locus_tag="PXA33_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005609574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HDL5119715.1"
                     /translation="MNLTPFFIAFRYFRSKSADRFGRLVTNLASLGIVLGVMALVIVL
                     SVMNGLENMQKTNLLSTLPHAIITPQEGHFAKGEKLALPEFAKQSVVINRANVIIQSS
                     QGINAGQLIGVENATDDPLLVAENIPALLPSGEFKVVIGSRLANKLNVTVGDKLRLMI
                     TENSQYTPLGRVPVQRLFEISGVYQSNREASEFTLFANLADVGRLLRISEDQVQGVRL
                     YLQDPFQVTELSQYFDAERYQISDWREQKGEFFQAVKMEKNMMGLLISLIIVVAISNI
                     VTSLSLMVVDKQGEIAILQTQGLTKKQVMQIFVFQGAIVGVIGSIIGGLLGVLIAYNL
                     DQIILLLNPSIHLPTLISGTQIAVIVGVSMLLSLLCTLYPAYRASKIEPAQALRYE"
     gene            10979..11662
                     /gene="lolD"
                     /locus_tag="PXA33_000342"
     CDS             10979..11662
                     /gene="lolD"
                     /locus_tag="PXA33_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HDL5119716.1"
                     /translation="MTELLRCEHISKFYDEGEQKVQVLKEVSFSMNEGELVAIVGSSG
                     SGKSTLLHTLGGLDQPSTGEVWIRGQSLQQLSSDKLALLRNQNLGFVYQFHHLMADFT
                     ALENVMMPMLIGKQNKTEAANRAEKMLQAVGLAHRMTHRPSALSGGERQRVAIARALV
                     NNPALVLADEPTGNLDQKTTESIFELIQQLNQEQNIAFLLVTHDLNLANKLSRSLVMR
                     DGVLSEGSL"
     gene            11659..12924
                     /gene="lolE"
                     /locus_tag="PXA33_000343"
     CDS             11659..12924
                     /gene="lolE"
                     /locus_tag="PXA33_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HDL5119717.1"
                     /translation="MSTPFFISWRYQRGKQKNKLVSLIALFSNIGIALGVAVLIIGLS
                     AMNGFERELNNRVLSVVPHAELVSFQGNQPQPIFDSKKLEALIKKSQNVTASSPFVSF
                     TALIENGAQLKIAQVRGVDPKKQDQASQLSQFIPPEQWQAFNQQFQADEDGLILGAGI
                     AKALEVEAGDEVTLLIPQPTEDGKLAQPLRFNLNVTGILRLDGQLDHSYALIPLNKAQ
                     ELMEYQANEVSGLEISLANPFDVQQLEMPQLNGYTQPLYLNTWIDKFGYMYNDIQLIR
                     TVMYIAMVLVIGVACFNIISTLIMAVKDKQGDIAILRTLGANNGFIRRIFLWYGLISG
                     MKGALFGLILGVILSLNLTAIIKAIEAFFEVKLLSDGVYFVDFLPSELHWQDVVYVLA
                     ATIILSLFASLYPANRAAKLEPAKVLSGH"
     gene            complement(12999..14930)
                     /gene="thrS"
                     /locus_tag="PXA33_000344"
     CDS             complement(12999..14930)
                     /gene="thrS"
                     /locus_tag="PXA33_000344"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011200229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HDL5119718.1"
                     /translation="MPIITLPDGSQRSFDNPVSVMEVAQSIGAGLAKATIAGRVNGER
                     RDASDIISQDSTLEIITAKDEDGLEIIRHSTAHLLGHSIKQLFPDVKMAIGPTIENGF
                     YYDIDLDRSLTQEDLDALEKRMLELAKTNYDVVKRPVSWQEARDTFEKRGEPYKMAIL
                     DENIERTATPALYHHEEYIDMCRGPHVPNMRFCHHFKLQKVAGAYWRGDSKNKMLQRI
                     YGTAWADKKQLAEYLQRLEEAAKRDHRRIGKALDLYHMQEEAPGMVFWHNDGWTIFRE
                     LETFVRTKLKEYDYQEVKGPFMMDRVLWEKTGHWQNYADLMFTTQSENREYAIKPMNC
                     PGHVQIFNQGLKSYRDLPIRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IESEVISCIKMVYDIYSTFGFTNIAVKLSTRPENRIGDDAMWDRAEKGLANALRNNGL
                     EYEIQEGEGAFYGPKIEFALRDCLGREWQCGTVQLDFALPGRLAASYVAEDNGRRTPV
                     MIHRAILGSIERFIGIITEEYAGFFPTWLAPTQAVVMNITDSQADYVQQVVKQLSDAG
                     IRVKADLRNEKVGFKVREHTLRRVPYMLVCGDKEIAEGKVSVRTRKGADLGTYLVSDL
                     VEILKNQIKARELKLLGEE"
     gene            complement(15097..16446)
                     /locus_tag="PXA33_000345"
     CDS             complement(15097..16446)
                     /locus_tag="PXA33_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL5119719.1"
                     /translation="MNSTKFYSLYSGIFFLFFGYGLFLNSAGIKLAEMGITDIIIDLL
                     NAAFFIGAALSTIFAHRIVSSVGHIRSFSVFCAVFAIAALGHIMIDTLWIWGVLRIIL
                     GFCHYSLLLLVESWLSEKTNVDTRGKVLATYNIIFYLAFILGVSLLSLELSSRNIFIL
                     STILVVMSMIPIALTKMVQPDIPARERISIPKLFAISPLALATSFISGMLVNGFFTMA
                     SVFLLKFSFSLSEISLYLMISMIGGFISQMPMAILSNRIGRRNAILACSVIATLTALA
                     GLFAIFQENLTAWGQYIIAFFFGCSLFSLYALSIARANDELPNNMNTVEVSRGLLFCY
                     GVGALVAPPLIGFAMSLSAEYGFYALFGLFSLSLFICAFRTKSIPKAERAEMQYATPI
                     ATASPLEEIEVVEDALIPFDEEAVQEYQAQLEILEQDPQTENENIELDEGAAENSKV"
     gene            complement(16721..17212)
                     /locus_tag="PXA33_000346"
     CDS             complement(16721..17212)
                     /locus_tag="PXA33_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HDL5119720.1"
                     /translation="MFTTQLIQTKRWLEANSITTFWLKTFIGATLLAIFAQISVPMYP
                     VPITGQTLALTVVGFAMGRKAATAAVLLYLFEGAIGLPVFAGGASGFHHFFGPTGGYL
                     LGYIPTAYFLGYYSDKGVLNSFWKSLFVALIASVITFAFGLAQLSLYQKAQYYKRAYI
                     HLF"
     gene            17293..18060
                     /locus_tag="PXA33_000347"
     CDS             17293..18060
                     /locus_tag="PXA33_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--protein ligase"
                     /protein_id="HDL5119721.1"
                     /translation="MVKPVYIYCDEGSSEIGVQSLLLAVKQHLQRPAYLINAKQIIQS
                     KFSDGCLLIMPGGADLPYCKKLNGEGNRRIRQFIEQGGAYLGICAGAYYGCAELNFVG
                     RDYSVNGNRELALFNGIAGIAKGSLPQFTNGQLYDKNIGSKAIVEVTLAEGGKMPFYY
                     HGGCCFEPNSTTVIYNPLAYYPDGSVAMIEGELGRGYYLLSGVHFELQAVVYWQKIVN
                     CCENLDIKTQEEAICSNFNVNYGSQIWQHLKQRLNQA"
     gene            complement(18051..18845)
                     /locus_tag="PXA33_000348"
     CDS             complement(18051..18845)
                     /locus_tag="PXA33_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HDL5119722.1"
                     /translation="MRFFDTHTHLDYLAESLNLSIPELVENAKQHQVECILIVAVFAK
                     NFAKVTACAKQKPENLVYGLGLHPLYIQQHQQQDLILLENLLSQQDPQCTAIAEIGLE
                     KAVSEVSTPELWKKQCEFLEAQLSLAKKFNLPVNLHSRKSHDQLFVFLKKAQLTKTGV
                     VHGFAGSYEQAKRFVDLGYKIGVGGTITYERANKTREAIRKLPLESLLLETDSPDMPV
                     FGFQGQPNRPERLLVTFNSLCELRTESPNLIAETIWQNSERLFFYA"
     gene            complement(18906..20492)
                     /locus_tag="PXA33_000349"
     CDS             complement(18906..20492)
                     /locus_tag="PXA33_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005667512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HDL5119723.1"
                     /translation="MLWFFFCVAVLVLGYFTYGKIIEKIFVINPKRATPAYAMNDGVD
                     YMPMSKTKIWLIQLLNIAGTGPIFGPILGALYGPVAMLWIVIGCIFAGAVHDYFCGML
                     SIRNGGATMPNLAGKFLGRPVKLFINVMALILLLLVGVVFVASPAQLLSTITMDIAGA
                     TQSAMELGDVEAIKESVASGGVTVWGMDKATVISVWTLIIFAYYILATLLPVDKIIGR
                     IYPFFGALLLFMSVGMVYGLVSAHLSATDPIDFFRTVNADGQGLTWEKFTQNFQVKGD
                     VPIWPLLFLTISCGALSGFHATQTPLMARCAQNEKEGRFIFYGAMITEGVIALVWCAV
                     GLSFYENPQALQDAISAGSPSKVVYDSSLHFLGFIGGIFAVLGVVVLPITSGDTAFRA
                     ARLQLAEIFGLEQRTLVKRLYIAVPLFVLGFIVSKVDFSVLWRYFTWANQMTAMVMLW
                     TAAGYLYRYKKFHWVCSIPAWFITTVCATYLANNKIGFGLDYELSVYIGFAITVVCVI
                     CFFAFLKPSEARDEDAYVTN"
     gene            complement(20678..22531)
                     /gene="dxs"
                     /locus_tag="PXA33_000350"
     CDS             complement(20678..22531)
                     /gene="dxs"
                     /locus_tag="PXA33_000350"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HDL5119724.1"
                     /translation="MEKKYPLLSEINSPEDLRLLSKDQLQTVVDELRAYLLESVSQTS
                     GHLASGLGVVELTVALHYVYQTPFDQLIWDVGHQAYPHKILTGRRDQMHTIRQKDGIH
                     PFPWREESPYDVLSVGHSSTSISAGLGIAVAAEKENAGRKTVCVIGDGAITAGMAFEA
                     LNHAGALHTDMLVILNDNEMSISENVGALNNHLARIFSGSLYTSVRDGSKKILDKVPT
                     IKNFMKKSEEHMKGVIFSPESTLFEELGFNYIGPIDGHNIDELVKTLSNMRELKGPQF
                     LHIRTKKGKGYTPAENDPIGYHGVPKFDPTAAELPKSKAAPTYSNIFGDWLCEMAERD
                     PKIIGITPAMREGSGMVEFSKRFPEQYFDVAIAEQHAVTFGAGLAIAGYKPVVAIYSS
                     FLQRAYDQLIHDVAIQNLPVIFAIDRAGIVGADGQTHQGAFDLSFMRCIPNMTIMAPS
                     DENEMRKMLYTAYTMNTPSAIRYPRGNAKGVKLEPMQRLEVGKGNIIREGKKVAILNF
                     GALLDEAKIVAEKHNYTLVDMRFIKPLDEALLQKVADSHELLVTLEENAIQGGAGSFV
                     NEYLQNIGKIKPLVMLGIPDFFVPQSTQAEAYADLGLDAQGIEAKIQKFDI"
     gene            complement(22576..23388)
                     /gene="suhB"
                     /locus_tag="PXA33_000351"
     CDS             complement(22576..23388)
                     /gene="suhB"
                     /locus_tag="PXA33_000351"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012621556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HDL5119725.1"
                     /translation="MNPMLNIAIRAARKAGNIIAKGYEQSPQDTQVLQKNTNDYVTEI
                     DKAAEAAIIDVIRKSYPEHSIVTEESGVLNGSNENVQWVIDPLDGTTNFVKRLPHFAV
                     SIAVRENGRTTVGVVYDPIRNELFTAVRGEGAKLNEFRLRIDAERRDLKGTVIATGFP
                     FKVIRHRDAHLNMLEGLMNNGVADFRRTGSAALDLAYVAAGRVDGFFEIGLKPWDFAA
                     GELIAREAGAIVTNFVGGTDYLKTGNLVVGNGRVVKEILNSIQPTLTEELKA"
     gene            23569..24603
                     /locus_tag="PXA33_000352"
     CDS             23569..24603
                     /locus_tag="PXA33_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA methyltransferase"
                     /protein_id="HDL5119726.1"
                     /translation="MQFKENNQPRFKHREERRSDECSPRFSDKGKMRGNEGKSDRPHK
                     LHFKRDEAYSRSEQIYPAEGKKASGEGSVQIWVKGGNSSVKEKKNGPLSPRAPEKIKK
                     NRLEEMKVYGENACISLFQQRPESIVRLWATVEGAKKLGDLMSYLAQHKKAYHVIDRE
                     EMERVTGTEHHGDMCLLVKKTASFVLEGYLQVPKKQDCLVLLDGVNNAQNVGGIIRTC
                     AFYGVKGIITENVDSLNSSAAARVAEGGLEFVRALETKNKHIALVQLRQAGYQIVHLT
                     RHKQAPSLTKIKLAEKVVFVLSEIPASDVAYAEDTTIQLSFNNPLNSGLNVAVNAGIL
                     LNQWYQTHIL"
     gene            24613..25497
                     /locus_tag="PXA33_000353"
     CDS             24613..25497
                     /locus_tag="PXA33_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HDL5119727.1"
                     /translation="MNQQKPFEVIAIVGTPRHDSAFETHLAVYNWLKDRDYQILVETE
                     VATQLQLKESYSLDQIGQRAELVIVIGGDGNMLGIARALAKYRVPLIGINRGNLGFLT
                     DIAPQTAFEQLYSCLVKNEYVIEERFLLEAFIERNGKIIAANNALNEIVVHPTQVARI
                     IEFEVYIDGKFAFSQRADGLIVATPTGSTAYSLSAGGPILTPNMNAMALVPMHPHTLS
                     SRPLVVDGDSQISLRFAKYNTPDLEVICDGQVNMHFTPEDRVLVRKSPDKLQLLHLKD
                     YSYFTVLGSKLGWASKLF"
     gene            25636..26613
                     /locus_tag="PXA33_000354"
     CDS             25636..26613
                     /locus_tag="PXA33_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1852 domain-containing protein"
                     /protein_id="HDL5119728.1"
                     /translation="MTTNFKCQVHTIEFNEDYQPEQSTRLTTNFANLARGENRKQNLQ
                     NAIAMINNRFNDLANWDNPNADRYSVKLDIVSLDIDVEGKGEFFPAIEWLKTYVIDYK
                     TGKTIDGIIGNNFSSFVRDYDFSVLLLDHNKDKPQFSVPEGYGELHGKLFKHLLASPE
                     YTAKYKKPPVICLSVATTRTYTRTTNVHPILGVEYLQDSYSLTDAYFAKMGLKVRYFM
                     PPKSVAPYAFYFDENADLLNDYTNLELISTIATMEAFQKIYRPEIYNANSVAGQVYKG
                     SLTYQDYSVTKVEYDREERTQLAIKQGKYTEEHFIKRYQAILEAWATEE"
     gene            26701..27732
                     /locus_tag="PXA33_000355"
     CDS             26701..27732
                     /locus_tag="PXA33_000355"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HDL5119729.1"
                     /translation="MSNILLPTSTAGSLPKPSWLAEPEKLWSPWKFEGEELLQAKRDA
                     LLVSLAEQIAAGIDIVSDGEQTRQHFVTTFIEHLEGVDFSKREIVRIRNRYDASVPSV
                     VGAVSRPKSVFVDDAKFLRAHTDKPIKWALPGPMTMIDTLYDGHYKSREKLAWEFAKI
                     LNEEAKELEAAGVDIIQFDEPAFNVFFDELNDWGVAALERAAEGLKCETAVHICYGYG
                     IKANTDWKQTLGNEWRQYEESFPKLQQSKIDIISLECQNSRVPMDLIELVRGKKVMLG
                     AIDVATNIIETPEQVADTLRKALQFVDADKLYPSTNCGMAPLSRQVAQGKLQALSQGA
                     AILRAEFAK"
     gene            27820..28041
                     /locus_tag="PXA33_000356"
     CDS             27820..28041
                     /locus_tag="PXA33_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5119730.1"
                     /translation="MLNLFKKPIKKSSFEAWAKLMDDVAKVAILAIPVILYGEYSLLM
                     KFTHSITLFAVVYFLLATARLFRETAEEM"
BASE COUNT         8585 a         5702 c         6082 g         7759 t
ORIGIN      
        1 agcatttttt taggattttc gatcaagatc acaaaaataa atttgcgagc aaggcaatgc
       61 ccttggttcc tcaaagggat taagagcatt aatcaacata attttttcat attgatgcaa
      121 atcttgcttg gctattttgg ctaaaacgac tttcccttca tcaagcaatt tactaagctg
      181 agtgcctttg agcaaataat gttgcgggct ataccagatt ccttgtttta ggaacagtaa
      241 attgccgatg gagcaatcac tgacgtagcc attattgaga ataatcacct cttcattttg
      301 taaaataggg agcaaatcaa accgcttgcg gtcgctaaat ttgaggtgat aatcccaatt
      361 ttccacctca accaagcgaa aacgttctac ttttttgggc gaatagataa aaaatttcag
      421 ctcaaattct gttgcgttgt aatctattcg gcaacgtact ttgccccgtt gagaagcctc
      481 tggcacaact aaaatatcag caaggctaaa acgaggttca cagccaaaat attcttgtgt
      541 tgcttgctca aaacgttgtt gatgatactg taagttctga actttgccgt caataatcga
      601 aagggtttca aatagcggaa atcggcacat agactttctc taacagttca ttatattcat
      661 cagttaacac actttgtgag gtaatgccac caccactatg aaacacaagc ccgtttgagg
      721 tttgagcaat gtaacgaatt gccaccgcac tttctaagct ctcgccatca aaataaccaa
      781 aaataccggt gtagtaaccc cgttttccta attctgcttg ctgaataatc gaaaccgttt
      841 tctctttcgg tgcaccgcta atggagccgg caggtagtaa ttttgccaat atcgaaccga
      901 tattttcttg ccaattttca cctaattcgc cacagatctc ggaacttgtc tgcaaaattg
      961 cccctcgatg ggtttctact ttttccacat aacggtattt agtaacttca atatttttcg
     1021 ccaccaacgc caagtcattt cggatcaaat ccacaatggt attatgttcg gtaaactctt
     1081 tttccgaagc cattaatttc tgctcggcat tctcctcatt tgcattaatt gtgcctttca
     1141 tcgggtagga aaaaatgcgg ttattttcaa ttcgcacaaa gcattcaggg gaaaaacaga
     1201 cgaacctgcc ttgcaaataa catttatatt ttgctttgct ggcggcaaaa atttccgcca
     1261 atgaataatt ggtttcaatg gcagtcgggt agctcaaatt cagcaagtag ctattgccgg
     1321 cttgaatttc ctttttcact aattcaaacc cggtttggta ttcgctaaac ggaatcggat
     1381 ttgccgataa ttcaaaagct ttagtcggtt tttcaaccgg cttttcgaga ttcgatttgc
     1441 aaaaaaattc aaaaaaaaga ccgcttgcgt gagcctgttc tagcggaaaa ataagtggct
     1501 tttgctgctc aaaatcaatt aaaaagaaaa aagggcgttt tgcttgcccg tagtgattgg
     1561 cttggtgaat aaaatcttgc atagaaaaca caatggctga aattggctga atcatcaaaa
     1621 gctctgttat aatgcaaagc aatagatttg aaaagcattt tttaaaaaag gttttaccga
     1681 tgaacaacac cattcatttt caagaaattg ggatctacat cgcccttgcc gtttgtgtgc
     1741 tggtaggcgt ttatctcttt ttcttgaaat cccgttatca gcgaaatgcc tacgaattaa
     1801 gccaagatct tggcaaaatc accgaaaatt ttgaacaaat tcagcaaaaa tatgaggtgt
     1861 tggctcaaga aaaaaatcag cttgagcagt gggcaattca acaacagacc aaatatgaag
     1921 cagtgagtga acgtttaaac gagcgagatg ggcaattgca acgattccaa caaaaaatcg
     1981 aacttgccga acagcaagaa aaccagctgg aacgctatat taatgagcta aaagaacgtg
     2041 tgggttcttc ccaagcaaag gcagaaagtt tggaagaaca gctacaattt agccaaagtt
     2101 cactctcaac taaagagcgg gaaaatcaag cgttatttag ccgtttaaac gaggtacaaa
     2161 acgagctgac cgaactgcgt actacgcttt cggaaaaaca ggcaaatttt gaagcacaac
     2221 aacgcaattt tattgaggta aaacaacagc ttaatgtgga atttcaacat ttggcacaac
     2281 aaattcttga cgaaaaaagc aagcgtttta gcgaaaccaa tcaaagctcg ttagaagctc
     2341 tactgaagcc gttcaaagag caaattgaag gctttcaaaa acgggtgaat gaggtacata
     2401 gtgagtcgct caaaggctcg gcaaatttag aggctgaaat taagcgggtg ctacaaattg
     2461 gcgtttcaat gtcggaagag gcacaaaatc tggcaacggc attaaaaggc aataataaaa
     2521 ttgccggcaa ctggggtgaa gtacagcttg aaagtgcctt acaatctgcc ggtttactcg
     2581 caggcgagca ttaccaagcc caagagagtt ttcgtgatga agaggggcga cgttttgccc
     2641 ccgattttgt ggtgcatttg ccggatcaaa aacatctgat tattgacagt aaagtctcgt
     2701 tagtagcgta cgatcaagcg gtcagatcag aagaaaattt tgcaatttct caagccttag
     2761 acgaacattg ccgttcgcta cgcaatcata ttgagggatt gtctaagaag aactacagtg
     2821 cattattagg cattaaaagc ccggattttg tactgatgtt tattccgata gaaccggctt
     2881 atattgaagc gatgaagcac gatccgcaac tgtttaactt tggctatgaa cgcaatgtga
     2941 ttttggtttc tcacaccacc ttaatgccga ttttacgcac ggtcgccaat ttatggcgga
     3001 ttgaacgagg caacagcgag gctcgtgaaa tcagcgaaaa agccggcgaa atttacaacc
     3061 aagtgtgtgc ggttgccgat cggttagcta aattaggtaa cacgctaaat accgcaaata
     3121 atcaatacaa tcaaaccgtt acctcgttag tgggacggca aggcttactg ggcaaagtgg
     3181 aacgattcca acatctttca agcaaagcaa gccaaattgt gccgcaggtt gagctgattg
     3241 aaaatgaggt ggatactatt aaattggaaa tggtatcgag agaattattg gaagaatagt
     3301 caaatctctt tattccctac aaaaatatgg ggtgatttga aatactaaag aaaataataa
     3361 aatgctaaaa aaactattaa tcatcgacaa ccacgactct tttaccttca acttggtgga
     3421 tttactccgt aagcttgatg tgccggcgaa agtggtgctg gttgaagagc ttgacctcaa
     3481 tgaggtggag cattttagcc atattttgat ttcgcccggc cctgatgtgc cgagagccta
     3541 tctacaaacc tttgcgatgt tggagcgtta tcatcaaacc aaatcaattc taggcgtttg
     3601 cttggggcat caaacgattt gcgagttttt tggcggcaag ttgtataacc taaacgatgt
     3661 tagacacggg cagcaacgca cgctcactca aattgagcct aaccccatat ttaaaggttt
     3721 gcctaagcag ttgaaagttg ggttgtatca ctcttggggc attttgcaaa attcgttgca
     3781 aaatacaccg cttgtaccaa ccgcagtttg cgatcaaaat gtgttaatgg cgtttaaaca
     3841 ccaacatttg ccgatttttg gcgtgcagtt tcacccggaa tcttttatca ctgaatttgg
     3901 tcaacaaatg ttggcaaatt ggcttgagat tacacaagat ttttagcgtg aaatccgcta
     3961 taatccgtca gttatttacc cttttaaaat ctgtaggggc gaaaaatttt tcgccttact
     4021 taatatgcaa gagataattt aaatgaaaga ttcgattatc aataaattag aaagtttaag
     4081 tgaacgccac gaagaactac aggcgttact tggcgatgcc tcaattattt ccgatcaaga
     4141 caaattccgt gcttattcca aagaatattc gcaattagaa gaagtggttg gcacctttaa
     4201 ccgctggaaa aagctgaaca atgatattga agaggctcaa ttattactcg atgatcctga
     4261 tatgaaggat atggcagcgg aagaaattgc ggaaaataaa gccgaaatcg agaatcttga
     4321 gcaacattta caaattttat tattgccgaa agatccgaat gatgaataca acgccttcct
     4381 tgaaatccgt gccggaacgg gcggtgacga ggctggtatt tttgccggtg atttataccg
     4441 tatgtacagc cgttattgcg aaagtaaacg ctggcgaatt gaagaaatgt cggcaaatga
     4501 gagtgaacaa ggcggatata aagagatcat cgttaaaatt tcaggcgaag gcgtttacgg
     4561 tcagcttaaa tttgaatcgg gcggacaccg tgtacaacgt gtaccgaaaa ccgaatcaca
     4621 agggcgtatt cacacctctg cttgcacggt agcggtaatg ccggagttgc cggaaagcga
     4681 gatgccggaa atcaatccat ctgatctgcg aattgacacc taccgctcat cgggtgcagg
     4741 cggtcagcac gttaatacca ccgactctgc agtgcggatt actcatatcc cgaccgggat
     4801 tgtggtcgag tgtcaagatg agcgttcaca gcacaaaaat aaagccaaag cgctggcagt
     4861 gttagcctct cggattgtac aagttgagca agaacgccaa gccgcagagc aagcggatac
     4921 ccgccgtaac ttacttggct caggcgaccg ttcggataaa atccgcacct acaactaccc
     4981 gcaagggcgt gtgactgatc accgtattaa cctcacggtt tatcgtttag acgaagtgat
     5041 gaacggcaaa attgatgaat tgatacaacc gattatcact gaatatcaag cggatcagtt
     5101 agcggcgttg tcggagcaaa attaatttta ttgtaagggt acactgtgtg tgcctgccaa
     5161 atataaattt gcaaaatttt gactattttt gatcgcttgt agtttctaaa aagtttaaaa
     5221 atttgaggaa aacaaaatga gaaagttagg tttattagca gtatttactt tagtattatc
     5281 agcctgttca aattcaacaa aaattgaaca aaccattctt tccaagcagg atatcgtagg
     5341 ctcttgggta tgcgctatta aatacgatga tctaaaagta agcactttag atttttctga
     5401 atttaaagct aatggcgaaa tgacgaattt cggtgatatt tctgatgaga attttgagcc
     5461 cattaaattt acttatgtaa caggagataa agggaagtgg gacttaaaag gaaatcgatt
     5521 agtgattgat tatgatcttt ctgaacgtaa agtaaaaaga acaacaccaa aaaaattttt
     5581 agagttcttg aaacaggaag agcgtaaagc ctcaaaaaca gctaaaaaac tccttaaata
     5641 tgaacaaggg attttcaata ttctgagtga tcagaaaaat actgaaaaca gtaaaataat
     5701 attagatatt ttgaagtttg gtcattcaag aatggcgatt cagcaaaata tgggagacaa
     5761 agtttattca ggtggttgtg taacagctga taaagcagaa gagtatttag atatcctaaa
     5821 aaatagaaat tctaaataat ggattaccga gagtggctca cttttgctga gcaaaaatta
     5881 cttgaaaata gcggggcaga tccataccta aacgcaaaat ttgatgcaaa tttgctattg
     5941 caagcagtca caaaacgttc aaaatcggcc atttttgctt tttcggaaac cctactttct
     6001 gaaagcgagc ttgcagaatt agcggaaaaa ttagctcggc gtgccaaagg tgaaccaatg
     6061 gcatatattt taggctatcg ggaattttgg tcgttgccgt taaaggtttc accggcaacg
     6121 ctgattcctc gccctgatac ggaacgactg gttgagttgg cgttggaata tgctaacaag
     6181 cggttaggaa atcagaaaat gttgcaaatt ctggatctcg gtaccggcac gggggcgatt
     6241 gcgttggcac tggcgagtga attaggcgag aaagcccaaa ttatcggggt ggatttccaa
     6301 aatgaggcgg tgcaactggc agaagaaaat cgccgaaact taggcttaaa tcaagttaca
     6361 tttttacaaa gcgattggtt cagttcgctt gcaaatcaac aatttgattt aattgtctca
     6421 aatccgcctt atattgatga atcggacgaa aatttgacgg tgggagacgt acgttttgaa
     6481 cctttaaccg cattagttgc cgataataag gggttgagcg atttgcaaaa aatcatcgaa
     6541 aatgcaccgc ttcacttaaa accgcaaggg gcgttaatgc tggaacacgg ctggaaacaa
     6601 ggtaacttgg ttcgagattt atttgattta cacttgtggg aacaggtaga aactgctcaa
     6661 gattatgccg gcaatgaccg agtaaccaaa gcgattttaa aataaggcag aaaatggacg
     6721 atattattga agaattgtta aaagagttgg aagatccgaa agtaaccatc agcgaaaccg
     6781 agctgaaaaa gtttctttat cgtgaaattt tacgattaac tacgctcatt gataagaatg
     6841 taagtgagca acaagtgttt ggtcagatgt ctgcgttggt gaaaaaagct cgctataacg
     6901 tgaatggcga aggcgatgaa gagagaatta accaattact tcatttagtc tatcgtgaat
     6961 ggggctttca ttgctactat gaagactatt tccacaccga aaatttgttg ctaaatcaag
     7021 taattcgcaa acggcgtgga atgccggttt cacttggggc ggtggcgctt tatttatccg
     7081 ccgtattgga tttgccgctt tacccggtta attttccgac tcagcttgtg ttacgggctg
     7141 aaatacacca gccgaacggg gcaaccaaag tgcgttttat caacccgtgg aacggtgaat
     7201 ttctcaccat cgaaatgctg aataaatggc tggaaggaga aatgggcttt ggcgtagaag
     7261 ttacccctga tttgttaaga cgagcagaac cacaagagtt gctggaaagg gtcgaaactg
     7321 tgtttaaaat ggcacttacc cgtgaaggca aatatgaaga aaccttacgc ttgattgaat
     7381 accgcttggc atttagcccg gaagatccgt acgaaatcag agaccgagga atggtactgg
     7441 caagtatgga ttgctaccaa gcagcattag aagatattaa ttatttcatt gaccaatgcc
     7501 ctgaagatcc ttctgccgaa attttaaaaa tggaagtgaa aggactgcaa caaaaaagcc
     7561 aagaaagtgt ggtgcattaa gctgagaagt agccccaaag gggctgagct atgattaacc
     7621 tagtacggct ctgccgtgct aggcagaaaa taagaaacaa ggactcccta tgacagataa
     7681 aattgtaaaa gttggcaata ttgaagtggc taatcacaaa ccgtttacgc tttttggtgg
     7741 tatgaatgtg ctggaaagcc gtgatatggc acttagagta tgtgagcaat atgttgaagt
     7801 cacgcaaaaa ctaaatgtgc cttatgtgtt caaagcctca tttgacaaag caaaccgttc
     7861 ttcaatccac tcttaccgtg gaccgggaat ggaagaaggc ttaaagattt tccaagaatt
     7921 aaaacaaacc tttggcgtga acatcattac cgatgttcac gaaatttacc aatgtaaacc
     7981 tgtggcagaa gtagttgatg tgattcaact accagcattt ttggctcgcc aaaccgattt
     8041 agtggaagca atggctcgta ccggtgcggt tattaacgtt aaaaagccgc aatttttaag
     8101 cccgggtcaa atggggaata tcgtagaaaa gattgctgaa tgcggcaacg atcaagtgat
     8161 tttgtgcgac cgtggcacta actttggcta cgataatcta gttgtggata tgcttggttt
     8221 cggcattatg aaaaaagtgt cgaaaggttg cccggtgatt tttgatgtga ctcactcatt
     8281 acaatgccgt gatccattcg gtgcggcttc cggtggtcgc agagatcaag taaccgaact
     8341 ggcacgctcc ggtatggcga ttggtttagc cggcttgttc ttagaagctc acccggatcc
     8401 aaactcggcg aaatgtgatg gtccatctgc attaccgctg tcaaaattag aagcctttgt
     8461 ttcacagatg aaagcgattg atgagttggt taagagcttt gaagaaattg atacttccaa
     8521 ataaactttc tccgcattaa atcctttcct actttctttg cttcgccaaa gaaagtaggc
     8581 aaagaaaggc gaccctactt cgctactttt cttcgcttag tgtgaatggg cttaacgaaa
     8641 aatttacatt cgcttcgctc agagtaaatt ttcctaccca ttaaaacacg ctcaggtagc
     8701 tcagaaggga gttagaaagg taaaagacag aataggagaa aaaaatgcta aatattgttt
     8761 ttcttgaccg taccggtatt cctgccactc acgatattcc tcgtccgaat tttccacata
     8821 cttggacgga atatgccacc acctctgccg atcaaacttt tgagcgggta aaagaagcgg
     8881 atattatcat taccagcaaa gtgttactcc agcgtgactt gctggctaaa ttaccaaagc
     8941 tgaaattaat tgcgatcaca gcaaccggca cgaacaatgt ggacttagcg gcagcaaaag
     9001 agttagggat tgcagtgaaa aatgtaaccg gctattccag cgttaccgtg ccggaacacg
     9061 tcattggtat gatttactcc ctgaaacatc gtttagccga ttatcaccgt gatttaatta
     9121 cgtctgaccg ctgggcaact tgcggccaat tttgctatgt agattacccg atccacgatg
     9181 tgcgaggctc aaccttaggc attttcggta aagggaacat cggggctgaa gtggcacggc
     9241 tggcacaagc ggtcggaatg caggttattt ttgcagaaca tcaaggcgca actgaaattc
     9301 gagaaggcta caccgccttt gaagaggtat tcaaacaagc agatattatt tcactacact
     9361 gccctttaac cgagcagact caaaacctga ttaatgcaga aaccttagcc ttaatgaagc
     9421 cgaccgctta tattatcaat acgggtcgag gcccactgat tgatgaacag gcattgcttg
     9481 aggcattaga gcaaggcaag attgccggcg ctgcgttaga tgttttagtg aaagagccgc
     9541 ctgaaaaaga caacccttta atgttagccg caaaacgttt accgaattta ctgattaccc
     9601 cacacgttgc gtgggcaagc gattctgcgg ttacaacact agtgaataaa gtgacacaaa
     9661 atatggaaga gtttgtagca acgggtatgt agcttgaagt aggggctggt cctgtgccag
     9721 ccctaatatt aatagaatga gagggcgggt ataaaacccg cccctaccaa tatcatttta
     9781 gaaaaacaga tgaatttaac cccttttttt attgcttttc gctattttag atcgaaaagt
     9841 gctgaccgtt tcgggcggtt agtcaccaat cttgccagcc ttggcattgt gctgggcgta
     9901 atggcattgg tgattgtgct gtcggttatg aacggtttgg aaaatatgca gaaaaccaat
     9961 ctgctatcca ccttgccgca cgctatcatc acccctcaag aggggcattt tgccaaaggt
    10021 gaaaaactgg ctctacctga atttgccaag caaagtgtgg tgattaaccg tgcgaatgtg
    10081 attatccaaa gttcacaagg cattaacgcc ggtcagttaa tcggcgtgga aaatgccaca
    10141 gacgatccgt tgttagtggc ggaaaatatc cccgcattat tgcctagcgg tgaatttaag
    10201 gtagtaattg gctctcgttt agccaataaa ttgaacgtta cggtaggcga taagctccgc
    10261 ttaatgatca ctgaaaacag ccaatatacg ccacttgggc gagtgccggt acagcgtttg
    10321 tttgaaattt cgggcgttta tcaatctaat cgtgaagcga gcgaatttac cctctttgct
    10381 aatttagctg atgtcgggcg tttgcttcgc atcagcgaag atcaagtaca aggcgtgcgt
    10441 ttatatttgc aagatccttt ccaagttacc gaacttagcc aatatttcga tgcagaacgc
    10501 taccaaatca gtgattggcg tgagcaaaaa ggcgaatttt tccaagcggt gaaaatggaa
    10561 aaaaatatga tgggcttact catcagcctg attattgtgg ttgcaatttc caacattgtg
    10621 acttcactca gtttgatggt ggtagacaaa cagggcgaaa ttgcaatttt acaaacccaa
    10681 ggcttaacca aaaaacaagt gatgcagatt tttgtgttcc aaggggcgat tgtcggagta
    10741 attggctcga ttattggtgg tttgctcggc gtattgatcg cctacaattt agatcagatt
    10801 atcctgctat taaacccgag catccatctg cctaccctaa tttcaggcac gcaaattgcg
    10861 gtcattgtag gggtgtcgat gttgctttca ctcctttgca cgctttaccc ggcataccga
    10921 gcctcaaaaa ttgagccggc acaggcattg agatatgaat aataagaacg gaaataaaat
    10981 gacagaatta cttcgctgtg aacatatcag caaattttac gatgagggcg aacagaaagt
    11041 tcaggtgcta aaagaggtgt cattttcgat gaacgaaggt gagctggtag cgattgtcgg
    11101 tagttcaggt tccggcaaaa gcaccctatt gcacacatta ggcgggctag atcagccaag
    11161 caccggtgaa gtctggattc gagggcaatc tctgcaacag ctcagttcag acaaattagc
    11221 gttattgcgt aaccaaaact taggctttgt gtaccaattt catcacttaa tggcagactt
    11281 taccgccctt gaaaatgtga tgatgccgat gctgatcggc aaacagaaca aaactgaagc
    11341 tgccaatcgt gccgagaaaa tgctacaagc ggtcggatta gctcatcgaa tgacacatcg
    11401 cccatcagca ctttcaggcg gcgaacgcca acgggtggcg attgcccgag cgttggttaa
    11461 taatccggct ctggtacttg ccgatgaacc gaccggtaat ttagaccaaa aaaccactga
    11521 aagcattttt gagctgattc aacagctcaa ccaagagcag aacattgcgt ttttattagt
    11581 aacgcatgat ttaaatctag cgaataaact ttcacgtagt cttgtaatgc gagatggcgt
    11641 gttaagcgag gggagcttat gagtacgccg tttttcatta gctggcgtta ccaacggggc
    11701 aaacagaaaa acaaactggt ttcactgatt gctctctttt ccaacatcgg tattgccctt
    11761 ggggttgcgg tattaattat cgggctaagt gcaatgaacg gctttgagcg agagctgaat
    11821 aatcgtgtgc tttcggttgt gccacacgct gaactggttt cgtttcaagg caatcagcct
    11881 caaccaattt ttgatagcaa aaaattagag gcattgataa aaaaatcgca aaatgtgacc
    11941 gctagttcgc cttttgtcag ctttaccgcc ttaattgaaa atggggctca gcttaaaatc
    12001 gcccaggtgc gaggggttga tcctaaaaag caagatcagg ccagccaatt aagccaattt
    12061 attccacccg agcaatggca agcgttcaat cagcaatttc aagccgatga agatggcttg
    12121 attttaggag ccggtattgc caaagcgtta gaagttgagg caggagacga agttacgctt
    12181 ttaattcctc agccaacaga agacggcaaa ttagcccagc ccttgcgttt taacctaaac
    12241 gtaaccggca ttttgcggct agatgggcag ctggatcaca gttatgcctt aattccactg
    12301 aataaagctc aagagctgat ggaataccaa gcgaatgaag tgtcagggct tgaaatcagc
    12361 cttgccaatc cgtttgatgt acaacagctt gaaatgccac aacttaacgg ctatacccaa
    12421 ccgctttatt tgaatacgtg gattgataaa ttcggttata tgtataacga tattcaactg
    12481 attcgcaccg tgatgtacat tgcaatggtt ctcgtgattg gcgttgcctg ttttaatatt
    12541 atttccacct tgattatggc agtgaaagac aaacaaggcg atatcgcgat tttacggaca
    12601 ctgggggcaa ataacggttt tatccgccgc attttcttat ggtatggctt aatttcaggt
    12661 atgaaagggg cgttattcgg gctgatttta ggtgtaattt tatcgctaaa tttaaccgct
    12721 attatcaaag cgattgaagc attctttgag gtgaaattgc tttcagacgg ggtttatttt
    12781 gttgatttct taccaagtga attacattgg caagatgtcg tctatgtatt ggcagccacg
    12841 attatcctaa gcctttttgc cagcttatac cctgccaacc gagccgctaa attagaaccg
    12901 gcaaaagtgt taagtgggca ttaatcattt tcctattcta ataaaaaagc agctacccct
    12961 cgattatgtc agaaaggtag ctgcttttat ttatgttatt actcttcgcc taataatttc
    13021 aactcacgag ccttaatttg gttttttaga atttcaacta agtctgaaac gaggtaagta
    13081 cctaagtctg cacctttacg ggtacgcaca gacactttac cttcggcgat ttctttatca
    13141 ccgcaaacca acatataagg cacacggcgt aaggtgtgct cacgcacttt gaagccgact
    13201 ttttcgttgc gtaagtctgc tttcacacga atgcctgcat cagaaagctg tttcaccact
    13261 tgttgcacat aatctgcttg gctgtcggta atgttcatta ccactgcttg ggttggggca
    13321 agccaagttg ggaagaaacc ggcatattct tcggtgataa tcccgatgaa acgttcgatc
    13381 gagcctaaaa tcgcacggtg aatcattacc ggtgtgcggc gaccattatc ttctgctaca
    13441 taagaggctg ctaaacgacc cggtagagcg aagtcaagtt gcactgtgcc acattgccat
    13501 tcacgtccta agcaatcacg caatgcaaac tcaattttcg gcccgtagaa tgcaccttcg
    13561 ccttcttgaa tttcatattc aagtccgttg ttgcgtaagg cgttggctaa gcctttttct
    13621 gctctgtccc acattgcatc atcaccgata cggttttccg gacgggttga aagttttact
    13681 gcaatgttag taaaaccgaa ggtgctgtaa atgtcgtaca ccattttgat acagctgatt
    13741 acttctgact cgatttgttc ttcagtacag aaaatatggg catcatcttg ggtaaaacca
    13801 cgcacacgca ttaagccgtg taatgaaccg gacggctcgt tacggtgaca cgaaccaaat
    13861 tccgccatac ggatcggtaa atcacggtaa gatttcaagc cttgattaaa gatttgaacg
    13921 tgacccgggc agttcatcgg cttaattgca tattcacggt tttccgattg ggtagtgaac
    13981 attaaatctg cgtagttttg ccagtgtccg gttttttccc aaagcacacg atccatcata
    14041 aacggacctt ttacttcttg gtaatcgtac tctttcaatt tggtacgcac aaaagtttca
    14101 agttcacgga aaatcgtcca gccgtcattg tgccagaaca ccatacccgg tgcttcttct
    14161 tgcatatggt ataaatctaa cgctttaccg atacggcggt ggtcacgttt tgccgcttct
    14221 tccaaacgtt gtaaatattc ggctaattgt tttttatcag cccaagctgt accgtaaata
    14281 cgttgtagca ttttattttt gctgtcgcca cgccagtaag cacccgctac tttttgtaat
    14341 ttgaagtggt ggcagaaacg catatttggt acgtgtggac cacggcacat atcaatatat
    14401 tcttcgtggt gatataacgc aggcgttgcc gtacgttcaa tattttcatc taaaatcgcc
    14461 attttgtatg gctcaccacg tttctcaaag gtatctcgag cttcttgcca gcttaccggg
    14521 cgttttacca cgtcatagtt ggtttttgcc agctctaaca tacgcttttc aagagcgtct
    14581 aaatcttctt gagtcaaaga gcggtctaaa tcgatatcgt agtagaaacc gttttcgata
    14641 gtcgggccga tagccatttt aacgtctggg aataattgct tgatggagtg acctaacaag
    14701 tgagcggttg agtggcgaat gatctctaaa ccatcttcgt ctttggcagt aatgatttca
    14761 agtgtgctgt cttggctgat gatatcagac gcatcacggc gttcaccgtt tacacgaccg
    14821 gcaatggtgg ctttcgctaa acctgccccg atagattggg caacttccat tacagaaact
    14881 gggttgtcaa atgaacgttg tgaaccgtcc ggtaaagtaa taattggcat aataaatcct
    14941 tacagtggta atccatacga gagattactt gtgttgtttg aatgaaaaaa ccgcctacaa
    15001 gcggtctttt ttgattaaaa ttttgcaatt tgcttattat ttcccttaca agtggggaat
    15061 aaaagcactg aaattctagc ataaaacaaa gaaaaattaa actttgctgt tttcagcggc
    15121 cccttcatca agctcaatat tttcattttc tgtttgtgga tcttgttcaa gtatttcaag
    15181 ctgagcctgg tactcttgta ccgcttcttc atcaaatgga ataagtgcgt cctcaacaac
    15241 ttcaatttct tcaagtgggc ttgcggttgc aattggggta gcatattgca tctctgctcg
    15301 ttccgctttc ggaattgatt tggtgcggaa agcacaaata aataaactaa gtgagaacag
    15361 tccaaaaagt gcataaaagc catattcagc ggaaaggctc atcgcaaaac cgataagcgg
    15421 tggagcaact aacgccccca caccatagca aaacagtaaa cccctgctta cttcaaccgt
    15481 gttcatatta ttcggcaatt catcgttggc acgggcaatg ctcaaagcat ataagctaaa
    15541 cagactacag ccaaagaaaa aggcgataat atactgtccc caggcggtca aattttcttg
    15601 gaaaattgca aataatccgg caagtgcggt taaagtggca atcactgaac aagctaaaat
    15661 ggcgttacgt ctgccaatac gattcgataa tatcgccatc ggcatttggc tgataaaacc
    15721 accgatcatc gagatcatca aataaagcga gatttcactt aatgaaaagc taaacttcag
    15781 cagaaagact gacgccattg taaaaaagcc gtttaccaac attccgctaa taaagctggt
    15841 tgctaaggct aacggtgaaa tcgcaaatag tttaggaata ctaatgcgct ctcgagccgg
    15901 aatatcaggc tgaaccattt tagttaaagc aattgggatc atcgacatca caaccaaaat
    15961 ggtagataag ataaaaatgt ttcggctact aagttctaaa cttaataaac ttacgcctaa
    16021 aataaaggcg agatagaaga taatgttata agtggcaagc actttgcctc tggtatcgac
    16081 attggttttt tcgcttaacc aactttcgac taaaagcagc aagctataat gacagaatcc
    16141 caaaataatt cgtaacacgc cccagatcca caaagtatca atcataatat gaccgagagc
    16201 ggcgatagca aaaacagcac agaaaacgct aaatgaacgg atatgcccaa cacttgatac
    16261 aatgcggtga gcaaaaatag tgcttaatgc cgccccaata aagaaagcag cgttaagtaa
    16321 atcgatgatg atgtccgtta tccccatttc tgccagtttg atccctgctg aatttagaaa
    16381 taaaccgtag ccgaaaaaca gaaaaaagat acctgaataa agggagtaaa atttagtgct
    16441 attcatcaat gctccttcaa acattctgcc agtattaaat cattgtaccg atttaatacc
    16501 ttttaggcaa aaaagttatt attagcgttg agaaaataac ttttggctat tttgattaaa
    16561 aaatcaaatg aaggtgattt tgaaatgtag taaaaaatag agcaatcaga attgaaaaag
    16621 ggtttgccgt aacaaagcct ttagaaagat tagagtttcg agaagaattt gtagcttggg
    16681 ctaaccaaaa cacttgctag gagtgcttta agcacaccac ctaaaataaa tggatataag
    16741 cccgcttgta atactgtgcc ttctggtaca aagaaagttg agccaagccg aaagcaaagg
    16801 tgatcacact tgcaattaag gcgacaaata aacttttcca aaaagagttt aatacgcctt
    16861 tatcgctata atagcctaag aaataggcgg ttggaatata gcctaataag tatccgcctg
    16921 ttggaccaaa gaaatgatgg aaacctgaag caccgccggc aaagacaggt aaaccaatcg
    16981 ccccttcaaa aagataaagt aatactgcag cagttgcggc tttacgaccc attgcaaaac
    17041 caaccacagt taaagcaagt gtttgccctg taatgggtac agggtacatc ggcacagaaa
    17101 tttgtgcaaa aatagcaagc agtgttgctc caataaaagt tttcaaccaa aatgtagtta
    17161 tactattggc ttctagccaa cgtttagttt gaattaattg cgtagtgaac ataatcctct
    17221 cctttaatat ggttaaaatg ttctcaaccg attatataag aagatgtctt ttagggtaag
    17281 agtgaattta aaatggtaaa accagtttat atttattgtg atgaaggtag cagtgaaatt
    17341 ggtgttcaat cactgttatt ggcagtaaaa cagcatttac agcgtccggc ttatctcatc
    17401 aatgccaaac aaattattca aagcaagttt tccgatggct gtttattaat catgcccggt
    17461 ggagccgatt taccttactg caaaaaatta aacggagaag gcaatcgtag aatccgccaa
    17521 tttattgaac aaggtggagc ttatctcggt atctgtgccg gagcttatta tggctgtgct
    17581 gaattgaatt ttgtagggcg tgactatagc gtaaatggta atcgagaact agctttattt
    17641 aatggcattg cgggcattgc gaaaggcagt ctgccgcagt ttaccaacgg gcaactttac
    17701 gataaaaaca ttggtagcaa agcgatagtt gaagtaacac ttgcagaggg tggaaaaatg
    17761 ccgttttatt atcacggtgg ttgctgtttt gaaccaaatt caacaacagt gatttacaac
    17821 ccgttggctt attatcctga tggctcagta gcaatgatag agggagagct agggcgaggt
    17881 tactatctgt tgtcgggtgt acattttgaa ttacaagcgg tcgtttattg gcaaaaaatt
    17941 gtaaattgct gtgaaaatct tgacataaaa acacaagaag aagcaatttg ctctaatttt
    18001 aatgtaaatt atggctcgca aatttggcaa catctaaagc aacgactaaa tcaagcgtaa
    18061 aaaaataacc tttcgctatt ctgccaaatg gtttctgcaa tcagatttgg tgattctgtt
    18121 cttaattcac atagactatt gaaagtcact aaaagccgtt ccggtcggtt cggctggcct
    18181 tgaaagccaa acaccggcat atcgggcgag tcggtttcca gtagtaaact ttcaagaggt
    18241 agtttgcgga tagcctcacg ggttttgtta gcacgttcat aggtaatagt tccccccaca
    18301 ccaattttat aaccgagatc gacaaaacgt tttgcctgtt catagctgcc ggcaaagccg
    18361 tgaaccacac cggttttggt tagttgggct tttttgagga acacaaaaag ttgatcgtgg
    18421 cttttacgtg aatgaagatt aacgggtaaa ttaaattttt tcgcaaggga aagttgtgcc
    18481 tctaaaaatt cacattgctt tttccataat tcaggggtag aaacttcact taccgctttt
    18541 tccaaaccga tttcggcaat agcggtacac tgtggatctt gttggcttag caaattttcc
    18601 aataagatta aatcttgttg ttggtgctgt tgaatataca gtggatgcaa ccctaatcca
    18661 tacaccaaat tttccggttt ttgtttggca caagcggtca cttttgcaaa attttttgca
    18721 aatacggcaa cgataagaat gcattcaacc tgatgctgtt tggcattctc aaccaattcc
    18781 ggaatgctta aattcaggct ttctgcgaga tagtcgaggt gagtatgagt gtcaaaaaat
    18841 cgcataataa aaccaataaa aacggggact agattgtccc catgtttagc attttacgca
    18901 agcaattagt ttgttacata agcatcttca tcacgagcct cagatggctt gaggaaagca
    18961 aagaagcaaa taacacaaac taccgtaatc gcaaaaccga tataaactga taattcataa
    19021 tctaaaccga agccgatttt gttgttcgca aggtaagttg cacacacggt agtgataaac
    19081 catgcaggaa tcgagcatac ccagtggaat tttttatagc ggtataagta acctgcggca
    19141 gtccataaca taaccattgc agtcatttgg tttgcccaag tgaagtaacg ccataatacg
    19201 ctgaaatcaa cttttgagac aatgaaacca agtacgaata atggtacggc aatatacaaa
    19261 cgttttacta aggtacgttg ttctaaacca aagatttccg ctagttgtaa acgagcggct
    19321 cggaatgcag tgtcacctga ggtgattggt aatactacaa cgcctaatac ggcaaaaata
    19381 ccaccgataa accctaagaa gtgtaacgag ctgtcatata ccactttaga cggtgaaccg
    19441 gcagagatcg catcttgtaa ggcttgtggg ttttcataga atgaaagacc tactgcacac
    19501 catactaatg caattacccc ttcagtaatc attgcaccgt agaaaataaa acgaccttct
    19561 ttctcatttt gcgcacagcg agccattaat ggggtttggg ttgcgtggaa accggataat
    19621 gctccgcaag aaatagttaa gaatagtaat ggccaaattg gaacatcacc tttcacttgg
    19681 aagttttggg tgaatttttc ccacgttaag ccttgaccgt ctgcattcac tgtacggaag
    19741 aagtcgatag gatcagtcgc acttaagtga gcgctgacta gaccatatac catacctaca
    19801 gacataaaga gtaataatgc tccgaagaaa ggataaattc gaccgatgat tttatcaaca
    19861 ggtagtaatg tagcaagaat atagtaagca aaaatgatta acgtccacac agaaatcacg
    19921 gttgctttat ccatacccca aacggttacg ccacctgatg ctacagattc tttaattgct
    19981 tcaacatcac ctaattccat tgcactttgg gttgcacctg caatatccat ggtgatggta
    20041 gagagtaact gagcagggct tgcaacgaaa actacgccta ctaataaaag caagatgagc
    20101 gccattacgt tgatgaataa cttgactgga cgacccaaga atttccctgc cagatttggc
    20161 atcgttgcgc caccgttgcg gatacttaac ataccacaga agtagtcgtg tactgcaccg
    20221 gcaaaaatac aaccaatcac aatccaaagc attgcaactg gtccgtataa ggcaccaagt
    20281 attggtccaa aaatagggcc ggtaccggca atgtttaata attgaatcag ccaaatcttg
    20341 gttttagaca ttggcatata gtcaacaccg tcgttcattg cataagccgg cgttgctctt
    20401 ttcgggttaa tcacgaagat tttttcaatg atttttccgt aagtaaagta gccaagaaca
    20461 agcacggcta cacagaaaaa gaaccacaac atagtagaaa tcctataaat tgagataaat
    20521 gatcattttt agcagaactg ataaaggcgt gccattgtaa gtgaggagtg aggataaagt
    20581 aaaatgaatt gttagatttg tgtgaataat ttactgaaaa tgtgaggttc atcaaaaaat
    20641 ccctttgttc tgcgtcaatc aaagggactt tttaaagtta aatatcgaat ttttgaattt
    20701 tcgcttcaat gccttgggca tctaatccaa gatcggcata agcttcagct tgagtagatt
    20761 ggggaacgaa aaagtcagga ataccaagca ttacaagcgg tttgattttg ccgatatttt
    20821 gcaaatattc attcacaaaa ctacctgcgc caccttgaat agcattttct tcaagcgtaa
    20881 ctaacaactc gtgtgaatcc gccacttttt gaagtaatgc ttcatctaat ggtttaatga
    20941 aacgcatatc taccaacgtg tagttatgtt tttcagccac aatctttgct tcatcaagca
    21001 aagcaccaaa atttaaaatc gcaacttttt taccttcacg aataatgttt cctttaccta
    21061 cttcaagtct ttgcattggc tcaagtttta cccctttagc attgcctcga gggtaacgaa
    21121 tagcacttgg cgtattcatc gtgtaagcgg tataaagcat ttttcgcatt tcattttcat
    21181 cgctcggtgc cataatggtc atattgggaa tacaacgcat aaaacttaaa tcaaatgcac
    21241 cttggtgagt ttgcccgtca gcgccgacaa ttccggctcg gtcaatcgca aaaatcaccg
    21301 gcaggttttg aatagcaaca tcgtgaatta attggtcata agctcgctgc aagaagctgg
    21361 aataaattgc gactaccggt ttgtaacctg cgattgctaa gcctgccccg aaagtcactg
    21421 cgtgttgttc ggcaatcgcc acatcaaaat attgttcagg gaaacgtttg gaaaattcta
    21481 ccattcccga gccttcacgc attgccggcg taatgccgat aattttcgga tcacgctctg
    21541 ccatttcaca tagccaatcg ccaaaaatat tggaataagt aggtgctgct tttgattttg
    21601 gtaactcggc tgcggtaggg tcaaatttcg gaacgccatg gtagccaatc ggatcatttt
    21661 ccgcaggtgt gtagcctttg ccttttttcg tgcgaatatg cagaaattga gggcctttca
    21721 gctcacgcat attgctcaag gttttgacta actcgtcaat attatgtcca tcaatcgggc
    21781 cgatataatt aaagcctaac tcttcaaaca aagtgctttc aggggagaaa atcacccctt
    21841 tcatatgctc ttcgcttttt ttcataaagt ttttgatggt aggcacttta tctaaaattt
    21901 tcttactgcc gtcacgcact gaagtataga gcgaaccaga aaaaatgcgg gcaaggtggt
    21961 tgtttaatgc cccgacattt tcagagattg acatttcatt atcatttaag ataaccaaca
    22021 tatcggtatg caatgcacct gcgtggttta atgcttcaaa tgccattccg gcagtgattg
    22081 caccatcacc gattacacag acggttttac ggccggcatt ttctttttct gctgccaccg
    22141 caatgccaag tccggcactg atcgaagtag aagaatgccc aacgcttaac acatcgtaag
    22201 ggctttcttc acgccaaggg aaggggtgaa taccatcttt ttggcggata gtgtgcattt
    22261 gatctcggcg cccagtaagg attttatgtg ggtaagcttg atgaccaacg tcccaaatta
    22321 attggtcgaa aggcgtttga taaacataat gcaaagctac cgtaagctct actaccccta
    22381 agccagaggc taaatgtccg ctagtttgac tgactgattc caataaatag gctcgcagtt
    22441 catcaactac ggtttgaagc tggtctttag aaagtaaacg taaatcttcc ggtgagttga
    22501 tttcgcttaa cagaggatac ttcttttcca taatgtttta ttacccacaa ccatcaaata
    22561 atagatgata ttaaattaag ctttgagttc ttcggtcaaa gtaggctgaa tgctatttaa
    22621 aatttcttta accacacggc cgttaccaac aaccagatta cctgttttca agtaatctgt
    22681 tccgccaaca aagttagtca caattgcacc ggcttcacgg gcaataagtt caccggcggc
    22741 gaaatcccac ggttttaagc cgatttcaaa gaaaccatca acacgacccg ctgcaacata
    22801 ggcaagatct aaagcggcag aaccggtacg gcggaaatca gctactccgt tattcattaa
    22861 gccttcaagc atatttaaat gtgcatcacg atgtctgatg actttgaaag gaaaaccggt
    22921 tgcaattaca gtgcctttta aatcacggcg ttctgcatca atgcgtaaac ggaactcgtt
    22981 taatttagcc ccttcgccac gcaccgcagt gaaaagctcg ttacggatag gatcgtaaac
    23041 tacccctact gttgtacgtc cgttttcacg cacggcaatc gacacagcaa aatgaggtaa
    23101 gcgttttacg aaattggttg taccatctaa tggatcgatc acccattgta cattttcatt
    23161 gctaccgttt aacacgccgg actcttcggt cacgatcgag tgttcagggt aagatttacg
    23221 gatcacatca ataattgccg cttccgctgc tttatcgatt tcagtcacat aatcattcgt
    23281 atttttttgt aaaacttgtg tatcttgagg cgattgttca tagcctttgg caataatatt
    23341 gcctgctttt cgtgccgcac gaatagcaat gtttaacatt gggttcatat ttccaccaga
    23401 ttttaaagaa cataataaag cacgaattat aaagaatgta gcgaaataaa gaaacaacaa
    23461 gcggtctaat ctgtgaaaaa ttttgcaaaa aaatggcttt ttctaaccgc ttggagtttg
    23521 gattcgttat aatacctccc attcaagcca cccatttagg tactaaaaat gcaatttaaa
    23581 gaaaataacc aacctcgttt taaacacaga gaagaacgcc gttcagatga gtgttccccc
    23641 cgtttttcag ataaaggcaa aatgcgagga aatgaaggta aaagtgacag accgcataag
    23701 ctacatttta agcgtgacga ggcttattca cgctctgagc agatttatcc agcagaaggc
    23761 aaaaaagcca gtggtgaagg tagtgtgcaa atttgggtaa aaggtgggaa tagctcagtt
    23821 aaagagaaaa aaaacggccc gctttcaccg agagcgcctg agaaaattaa gaaaaatcgc
    23881 ttggaagaga tgaaagtcta tggcgaaaat gcctgtatca gtttgttcca acaacgtcct
    23941 gaatctattg tacgtttatg ggcaacagtg gagggggcaa aaaaattagg cgatttaatg
    24001 agctatttgg ctcagcacaa aaaagcctat cacgttattg accgtgaaga gatggagcga
    24061 gtcaccggta ctgaacatca cggcgatatg tgcttattgg tgaaaaaaac ggcttcattt
    24121 gtgctagagg gctatttaca agtgccgaaa aaacaagatt gcttggtact gttagatggt
    24181 gtaaacaatg ctcagaatgt ggggggaatc atcagaacgt gtgcttttta tggtgtgaaa
    24241 ggcattatta ctgaaaatgt ggatagctta aactccagtg ctgcagctcg tgtcgcagag
    24301 ggtggtttgg aatttgtgcg agcgttagaa actaaaaaca agcatattgc tttggtacaa
    24361 ctccgccaag ccggttatca aattgtccac ctcacacgcc ataagcaagc cccttcttta
    24421 accaaaatta agctggcgga aaaggtggtt tttgtattaa gtgagattcc tgcaagtgat
    24481 gtggcttacg cagaagacac aacaatacag ctttccttta ataatccgtt aaatagtggt
    24541 ttaaatgtgg cggtaaacgc aggtattcta cttaatcaat ggtatcaaac ccacattctt
    24601 taaggaattg aaatgaatca gcaaaaaccg tttgaagtga ttgcgattgt tggcacacct
    24661 cgccacgaca gtgcatttga aacacatctt gccgtttata attggttaaa agatcgtgat
    24721 tatcaaattt tagtggaaac agaggttgcc actcagttac agcttaagga aagctacagt
    24781 ttagatcaga ttgggcagcg cgcagagttg gtgattgtga taggtggcga tggtaatatg
    24841 cttggcatcg cccgagcttt ggctaaatac cgagtgccac tgataggcat taaccgtggc
    24901 aatctgggtt ttttaaccga tatcgcacct caaacggctt ttgaacagct ttatagttgc
    24961 ttagtcaaaa atgagtatgt gattgaagag cgattcctgt tagaggcttt cattgagcgc
    25021 aacggtaaaa ttattgccgc aaataatgcc ttgaatgaaa ttgtggttca cccaacccaa
    25081 gtggctcgaa ttattgaatt tgaagtttat attgacggaa aattcgcttt ttcacaacgt
    25141 gcagatggct tgattgtggc aacgcctaca ggctcaaccg cctattcact ttctgctggt
    25201 ggcccgattt taaccccgaa tatgaatgca atggcattag tgccgatgca ccctcacact
    25261 ctttcttctc gtcctttggt ggttgatggc gatagtcaaa tttcgcttcg ttttgccaaa
    25321 tataataccc ctgatttaga agtgatttgt gacgggcagg tgaatatgca ctttacgcct
    25381 gaagatagag tcttggtgcg taaaagcccg gataaattac agctgctcca tttaaaagat
    25441 tatagctact ttactgtatt aggttctaaa ttaggctggg caagtaagtt gttttaaaca
    25501 caagcggtgt tttttctgat taaatttgca aatgcaatat gaattgattt catatacatt
    25561 tcaaaaatat atcattttta ttcaataaaa atttgtggtt caataagcta tctgaaaaat
    25621 aaaggggaca ataaaatgac aacaaatttt aaatgccaag tgcatactat tgaatttaat
    25681 gaagattacc aaccggagca aagcacccgt ttaaccacca actttgccaa ccttgcccgt
    25741 ggcgaaaacc gcaaacagaa cctgcaaaat gcgattgcga tgatcaataa ccgttttaac
    25801 gatttagcga actgggataa cccgaatgcg gatcgttatt cggtgaaatt ggatattgtc
    25861 tcactggata ttgatgtgga aggcaaaggc gagtttttcc ctgcgattga gtggctgaaa
    25921 acctatgtta tcgactacaa aaccggcaaa accattgatg gcattatcgg taataacttc
    25981 tcttcttttg tgcgtgatta cgatttcagc gtattgttgt tagatcacaa taaagacaaa
    26041 ccgcaattta gcgtgccgga aggctatggc gagttacacg gcaagctgtt taagcattta
    26101 ttggcatcgc ctgaatatac cgcaaaatat aaaaaaccac cggtgatctg tttaagcgtg
    26161 gcaaccaccc gtacctacac aagaaccact aacgttcacc caattttagg tgtggaatat
    26221 ctgcaagata gttattcatt aactgatgcc tattttgcga aaatgggctt aaaagtacgt
    26281 tactttatgc cgccaaaatc cgttgcaccc tatgcgttct actttgatga gaatgcagat
    26341 ttactcaatg attacactaa cttagagcta atcagcacga ttgccacaat ggaagcgttc
    26401 caaaaaattt accgtccgga aatttacaat gccaattcgg tcgcagggca ggtctataaa
    26461 ggcagcctga cttaccaaga ttattccgtc accaaagtgg aatatgaccg tgaagaacgc
    26521 acccagttgg cgatcaaaca gggtaaatac accgaagagc attttattaa acgttaccaa
    26581 gcgattttag aggcttgggc gacggaggaa taaacgtagg gtgggcgtaa gcccaccata
    26641 aaatgagata taaccaatgg tgggcttacg cccaccctac tttttatagg aaaattcaaa
    26701 atgagcaaca ttcttctccc aacctctacc gccggcagcc taccaaaacc ttcgtggctt
    26761 gccgagccgg aaaaactgtg gtcaccttgg aaatttgaag gcgaggaact acttcaagcc
    26821 aaacgtgatg ccctccttgt ttcccttgcc gaacaaattg cggcagggat tgatattgtt
    26881 agtgatggcg aacagacccg ccagcacttt gtgaccacct ttatcgagca tttggaaggg
    26941 gtggatttta gcaagcgtga aatcgtgcgc attcgtaacc gttacgatgc cagcgtgccg
    27001 agcgtggtgg gggcagtttc ccgcccgaaa tcggtgtttg tggacgatgc caaattcttg
    27061 cgtgcgcata ccgacaaacc aatcaaatgg gcattacccg gcccgatgac aatgatcgac
    27121 actctctatg acggtcatta caaaagccgt gaaaaattgg cgtgggaatt tgccaaaatt
    27181 ctcaacgaag aagccaaaga gttggaagcg gcaggggtgg atattattca atttgacgaa
    27241 ccggcattta acgtcttctt cgatgagctg aacgactggg gcgtggcggc gttagagcgt
    27301 gcggcggaag gcttaaaatg cgaaacggcg gtgcatatct gctacggcta cggcattaaa
    27361 gccaataccg actggaaaca gaccctcggc aacgaatggc gtcaatacga agagagtttc
    27421 ccgaaactgc aacaatcaaa aatcgacatt atctcgttag agtgccaaaa ctcccgtgtg
    27481 ccaatggact taatcgaatt agtgcgtggc aaaaaagtaa tgctgggagc gattgatgtg
    27541 gctaccaaca ttatcgaaac accggaacaa gtggcagata cgttacgcaa agcattacaa
    27601 tttgtcgatg cggataaact ttatccaagc accaactgcg gaatggcacc actttcacgc
    27661 caagtcgcac aaggtaaatt acaggcactc agccaagggg cagcgatatt gcgtgcggaa
    27721 ttcgcaaaat aattttgcga tctgcatcga aaatatcaac tttgcttttt gctcgtttcc
    27781 agattttctt ccaaaataac aggaaatgac ggaggcacta tgctaaatct atttaaaaag
    27841 ccaattaaga aaagctcctt tgaggcttgg gcaaaactaa tggacgatgt ggcaaaagtt
    27901 gcaattttag caattcctgt tattctctat ggagaatatt cactgctaat gaaatttaca
    27961 cactcaatta ccttatttgc agtagtttat tttttgttag caacggcaag attgtttcga
    28021 gaaacagcag aggagatgta aatggacgga ttgacaatcg gattatttct tttgcttgca
    28081 ttttcatttg ctgcatacca atgggcaaag catttaaaag atcataat
//
LOCUS       DANLRK010000012        26485 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.012, whole genome shotgun sequence.
ACCESSION   DANLRK010000012 DANLRK010000000
VERSION     DANLRK010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 26485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26485
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.012"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(31..821)
                     /locus_tag="PXA33_000357"
                     /pseudo
     CDS             complement(31..821)
                     /locus_tag="PXA33_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618283.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
     gene            complement(818..1519)
                     /gene="lpxH"
                     /locus_tag="PXA33_000358"
     CDS             complement(818..1519)
                     /gene="lpxH"
                     /locus_tag="PXA33_000358"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HDL5119731.1"
                     /translation="MTYYFIADLHLNENQPEITHLFLQFMQEKALLAEAVYILGDLFD
                     FWIGDDEQSELISTVKQAIKTLTTSGTKCYFICGNRDFLIGKRFSEQTGMVILPDYHV
                     IDLFGTSTLLCHGDTLCTDDVKYQQFRKKVHQKWRQAIFLMFPLSWRIRIAEKIRSKS
                     KQEKRDKSAEIMDVNAEVTAQIVAKYNTTQLIHGHTHRQAIHQHNGFTRIVLGDWKEN
                     YASILSVTEQGAEFI"
     gene            complement(1594..2115)
                     /locus_tag="PXA33_000359"
     CDS             complement(1594..2115)
                     /locus_tag="PXA33_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1523 family protein"
                     /protein_id="HDL5119732.1"
                     /translation="MRAILKYFLILVSLSFFIVLGGAVNYAMPSYEDTVVTGMEVRRM
                     DKDGIISKANPADGEVRDVYFLFTEDPETKKVMVYRNEDTGWGLPPYFKFGSADIQAK
                     AQAYANEKQRVQIKYYGWRINWLNEFRNIVSIKPLEEAETVSKPIMSYVLYAVLAFLF
                     FLSVQFIRGIFKD"
     gene            complement(2125..2913)
                     /locus_tag="PXA33_000360"
     CDS             complement(2125..2913)
                     /locus_tag="PXA33_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchF/TatD family DNA exonuclease"
                     /protein_id="HDL5119733.1"
                     /translation="MKDLFIIDSHCHLDSLDYETRHKNVDEVIENAKARGVHHFISVC
                     TTVGRFEAMKNLTAHRNDVSLSCGVHPLNVEDEPFDYDKLFQFAQDPKVVAIGETGLD
                     YHYTPETKALQQSLFVQQIEIANKLNKPLIIHSRSARQDTMDMLEQNNAEKCGGVLHC
                     FTEDWAMAKRALDIGFYISISGIITFRNAEELREVVRKVPLDRLLVETDSPYLAPIPY
                     RGKPNQPAYVRETCEYVATLKGVSTEELARITTENVQHLFNIKL"
     gene            complement(2918..3202)
                     /locus_tag="PXA33_000361"
     CDS             complement(2918..3202)
                     /locus_tag="PXA33_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL5119734.1"
                     /translation="MYKLSAQALRDQEKLFRYSISQFGVQQTESYFADLKHILNLLAD
                     FPTMGTSAEYIRGGLRMHTHQSHTIYYRIKADHIFIVRILHNQMNHKRYI"
     gene            complement(3195..3437)
                     /locus_tag="PXA33_000362"
     CDS             complement(3195..3437)
                     /locus_tag="PXA33_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HDL5119735.1"
                     /translation="MARTTSVTLGEPLNDFVNEMVASGRYGSTSEVVRTALRMLEEKE
                     QYLAQLRQAIDDGDASGYATETIDEMMLRVKAELNV"
     gene            complement(3568..4545)
                     /locus_tag="PXA33_000363"
     CDS             complement(3568..4545)
                     /locus_tag="PXA33_000363"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HDL5119736.1"
                     /translation="MYPWLESTYQQLTDSFLQGRGHHALLFKTDVGLGTDTLIYKVAE
                     WLLCSQKLRHQPCLECKSCLLMQSNNHPDFHILEPIEDKDIGIDQVRELIYKLQNFAQ
                     QGGNTVVYIRGVDRLTEASSNALLKTLEEPRENVYFLLEAPLQATVLATIQSRSQTWV
                     LYAPQASEVMAWLQTQFPAISTDDLEIALRLCHSRPLICKNFIENDRLQQRKTFLQTF
                     WRFYKSHDVFLLFQAFAQEKELILQQLEWLESFFSDALKAKMAISHGWLNPDLQNGIL
                     PFSQQLTTHGLLKGHQILQQTQRDLTEINAVNPELMLLDCLTKLVLVFE"
     gene            complement(4545..5165)
                     /locus_tag="PXA33_000364"
     CDS             complement(4545..5165)
                     /locus_tag="PXA33_000364"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HDL5119737.1"
                     /translation="MRGKFIVIEGLEGAGKTTAHQVILKELEQAGINDVVMTREPGGT
                     PLAEKLRHLIKHETEEAINDKAELLMLYAARVQLVENVIKPALAEGKWVLGDRHDMSS
                     QAYQGGGRKLDRHLLETLKESVLGTFEPDLTIYLDLDPVIGLERARGRGELDRIEQQS
                     LDFFYRTRERYLELTQHNDKAVIINAEQPIEQVTADIQQVIRNWLK"
     gene            complement(5176..6210)
                     /gene="mltG"
                     /locus_tag="PXA33_000365"
     CDS             complement(5176..6210)
                     /gene="mltG"
                     /locus_tag="PXA33_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005613374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HDL5119738.1"
                     /translation="MLKKILIALGLAGVIACGGLFYGYQKLTSLAEHPITVQPNQLFV
                     LEKGVSSQKLAALLEEQGIVTHDDADLIPYLMRLYPELSKFKAGAYSLAGLTTVKDLL
                     AHLSSGKEVQLNVQFIEGKTFKIWREQLAKADYLQHTLQGKSEKEIADLLGIPHEKIE
                     GWIAPDTYNYTPNSTDLALLKRAYQKQKTALEQAWKNRAENLPLATPYEMLILASIVE
                     KETGIATERPQVASVFINRLRNNMRLQTDPTVIYGMGDRYNGNIRRKDLEEATPYNTY
                     QIDGLPPTPIAMPSEASIKAVANPDNTPYLYFVADGTGGHKFSKTLNEHNKAVAEWIQ
                     IERKRKNEQK"
     gene            complement(6303..6378)
                     /locus_tag="PXA33_000366"
     tRNA            complement(6303..6378)
                     /locus_tag="PXA33_000366"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6343..6345),aa:Lys,seq:ttt)
     gene            complement(6400..6475)
                     /locus_tag="PXA33_000367"
     tRNA            complement(6400..6475)
                     /locus_tag="PXA33_000367"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6440..6442),aa:Lys,seq:ttt)
     gene            complement(6498..6573)
                     /locus_tag="PXA33_000368"
     tRNA            complement(6498..6573)
                     /locus_tag="PXA33_000368"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6538..6540),aa:Lys,seq:ttt)
     gene            6790..7317
                     /gene="ppa"
                     /locus_tag="PXA33_000369"
     CDS             6790..7317
                     /gene="ppa"
                     /locus_tag="PXA33_000369"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010944216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HDL5119739.1"
                     /translation="MGLELVPAGKELPDDIYVVIEIPANSDPIKYEVDKETGALFVDR
                     FMATAMFYPANYGYVNNTLSSDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGG
                     DAKVVAVPHTKLSKEYDHIKDVGDLPALLKAQIQHFFESYKALEAGKWVKVEGWGDVN
                     EARQEILESFERAKK"
     gene            7373..8128
                     /locus_tag="PXA33_000370"
     CDS             7373..8128
                     /locus_tag="PXA33_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HDL5119740.1"
                     /translation="MKFIKKLSYSVFLATVITACATTESINQEAAQSYAQVKTQARQQ
                     NAIDNSSATAKRIHTVFHKMKPYAERANQTGVPFHWEITVLKSDELNAWAMPGGKMAF
                     YTGLVERLNLSNDEIAVVMGHEMAHALKEHGKSDRTVSAITSIVGAIADVAVTASTGV
                     STEGLLSTGVDLIATKPFSRSQETEADEVGLILMAESGYNPSAAPNVWVKMSKANGDS
                     GLSIFSTHPSNADRQENLARLVPEAMKIYNARK"
     gene            complement(8192..9223)
                     /gene="trpS"
                     /locus_tag="PXA33_000371"
     CDS             complement(8192..9223)
                     /gene="trpS"
                     /locus_tag="PXA33_000371"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HDL5119741.1"
                     /translation="MSKPIVFSGVQPSGELTIGNYLGALRNWVKMQDDYDCVYCIVDQ
                     HAITVRQDPESLRKSTLDVLALYLACGIDPNKSTIFIQSHVPEHAQLAWVLNCYTYFG
                     EMGRMTQFKDKSARYEENVNVGLFTYPVLMAADILLYQANQVPVGDDQKQHLEITRDI
                     ANRFNALYGKKDSEGNLIEPVFAVPEVFIAKSGARVMSLLEPTKKMSKSDDNRNNVIG
                     LLEDPKAVAKKIKRAMTDGDEPPVVRYDVQNKAGVSNLLDILSAITGKTIAELETEFE
                     GKMYGHLKTEVADKVSELLTNLQERYHHYRQDEALLEKIYREGAEKARAQAKATLDKV
                     YELVGFVRY"
     gene            complement(9350..9682)
                     /locus_tag="PXA33_000372"
     CDS             complement(9350..9682)
                     /locus_tag="PXA33_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDL5119742.1"
                     /translation="MKRGDIYWLDLDPTLGHEQSGFRPVVIVSATAFNLASKLPVVVP
                     ITNGGAFAERLGFAVSLKDAGLKTTGIIRCDQPRTVDIAQRHGKFIEALPESLLDEIM
                     ARVSVIFE"
     gene            complement(9679..9939)
                     /locus_tag="PXA33_000373"
     CDS             complement(9679..9939)
                     /locus_tag="PXA33_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HDL5119743.1"
                     /translation="MPTIALRQVGGSVAMVIPPAFLEALKLSVGSKVDLSLNDNQLVI
                     NPARPKVKYSLEELLRQSDFEAARNEESQSWLSEPAIGKEIL"
     gene            complement(10009..10677)
                     /locus_tag="PXA33_000374"
     CDS             complement(10009..10677)
                     /locus_tag="PXA33_000374"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HDL5119744.1"
                     /translation="MSKKYKVIGFDLDGTLINTLPDLTLVANSMFLEAGLPTVTQEKV
                     LTWIGKGADIFFQNAVAHTGRIFQADELVKMRVSFDKYYATYVCEESTLFPNVKETLE
                     ILKANDYPLVVITNKPTKLVEPVLSAFGIYHLFDETLGGQSLPKIKPHPDPMFFICEK
                     FGITPSELLFVGDSENDVIAAKAAGCDVVGLTYGYNYNVPIEQSNPTFVTSEFKDVLK
                     IVGL"
     gene            complement(10764..11561)
                     /gene="lgt"
                     /locus_tag="PXA33_000375"
     CDS             complement(10764..11561)
                     /gene="lgt"
                     /locus_tag="PXA33_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HDL5119745.1"
                     /translation="MNEQFIQFPQISPIVFEIGPIALRWYGLMYLAGFAFAYWLGMRR
                     AKNSNGVWTTEQVDSLVYTCFWGVVLGGRIGDVFFYSFDRFLQDPLYLFRIWEGGMSF
                     HGGLIGVIVAMIWVSYRQKRNFWQTADFIAPLVPFGLGMGRIGNFINDELWGRVTDVP
                     WAVLFPSGGYLPRHPSQLYEAFLEGVVLLFILNWFIQKPRPAGAVSGLFLIGYGTFRF
                     IVEYFREIDHNVNTTADLITRGQLLSLPMIIGGILIMAWAYKVNKVK"
     gene            complement(11569..12357)
                     /locus_tag="PXA33_000376"
     CDS             complement(11569..12357)
                     /locus_tag="PXA33_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDL5119746.1"
                     /translation="MTILLSCLFLGVFVGFLAGLFGIGGGLIIVPSLVFLLPMAGVPP
                     EYIMSTALGTSFSTIIITAFASAQRHHKLGNVDIQTSKYFIPALMVSVFLSGLVVSRL
                     DPQLMSRIFAVMVLYLAGKMIFSIKRAPKNKPLTVQTTIIGGSIIGALASMAGIAGGA
                     FIVPFLNDRGLEMKRAIGTSSFCGAFLGLSGTISFIVSGWGLEGMPDYSLGYVYLPAL
                     FGITATSFFTSKMGASAANALPVPMLKKAFAVLLVCIAINMFLK"
     gene            complement(12359..13003)
                     /gene="rppH"
                     /locus_tag="PXA33_000377"
     CDS             complement(12359..13003)
                     /gene="rppH"
                     /locus_tag="PXA33_000377"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HDL5119747.1"
                     /translation="MIDFDGYRPNVGIVICNKHGQVLWAKRFGQNSWQFPQGGINEGE
                     NIEAAMYRELYEEVGLSKKDVRLLWASKYWLKYKLPKRLVRNEGSGPVCIGQKQRWFL
                     LQLIGDESLINLKTTKNPEFDGWRWVSFWYPVRQVVSFKRDVYRKAMKEFAQVLQNNI
                     QKATDDKTGEKPRIERGETSPHTYKKTSQDSAKAYKREARKPYRPWHQHNQKGK"
     gene            13615..14760
                     /gene="lldD"
                     /locus_tag="PXA33_000378"
     CDS             13615..14760
                     /gene="lldD"
                     /locus_tag="PXA33_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent L-lactate dehydrogenase LldD"
                     /protein_id="HDL5119748.1"
                     /translation="MIISSANDYRRAAKRRVPPFMFHYADGGSYAEQTLRRNVSDLED
                     IALRQRVLKDMSQLDTGIELFGEKLSMPVTLAPVGALGMYARRGEVQAAKAADNKGIP
                     FTLSTVSICPIEEVAPAIKRPMWFQLYVLKDRGFMKNALERAKAAGCSTLVFTVDMPT
                     PGARYRDMHSGMSGPYKDIRRVLQAMVHPFWAWDVGIHGKPHTLGNVSNYMGKPIDLN
                     NYIGWLTDNFDPSISWKDLEWIREFWDGPMVIKGILDPEDAKDAVRFGADGIIVSNHG
                     GRQLDGVLSSAKALPSIADAVKGEIKILADSGIRNGLDVVRMLALGADCTMIGRSFVY
                     ALSAAGQAGVENLLDIFLKEMKVAMTLTSNAKISDIGRDALVDLSNL"
     gene            complement(14814..15083)
                     /locus_tag="PXA33_000379"
     CDS             complement(14814..15083)
                     /locus_tag="PXA33_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5119749.1"
                     /translation="MLSEERFGFFYIFIFIYFSLLSLSIGYIRPELRTTYDYLEIENK
                     RYYVLSIHRNNIYVLGEKTKDNDEFLFFNQDTLKYYRINITKMPN"
     gene            complement(15158..15544)
                     /locus_tag="PXA33_000380"
     CDS             complement(15158..15544)
                     /locus_tag="PXA33_000380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5119750.1"
                     /translation="MTNQKFINSSLTLAFFTLISFSTAYCYAWGIAAFHGYPWWHVEV
                     GNAGIARALAYVFGTFLTIFLFYLIGYALVNKVFKLHYFKYLGWLRVSVLVTIFSLPV
                     MISFYLFIGKVPMYLHLLYLGTTTSI"
     gene            complement(15797..16627)
                     /gene="fghA"
                     /locus_tag="PXA33_000381"
     CDS             complement(15797..16627)
                     /gene="fghA"
                     /locus_tag="PXA33_000381"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014167586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HDL5119751.1"
                     /translation="MQQFENYRCFSGEQQVWQHFSASLNCEMKFAIYLPQSAKTQKLP
                     VLYWLSGLTCTEQNFITKSGFQRYADEHHLIVVAPDTSPRGVNVANDEAYDLGQGAGF
                     YLNATQGEWTTHYQMYDYIVSELPTLINAHFPTNGKQSIFGHSMGGHGALTIAFKNPG
                     QYQSVSAFAPIVAPTQVPWGQKAFLAYLGEDQTAWKAYDSVALLEKYRPSLPILIDQG
                     SQDSFLSDQLKPEIFCETADRLGIPYQFNLRDGYDHSYYFISSFIGEHIAFHAKHLKD
                     "
     gene            complement(16707..17765)
                     /locus_tag="PXA33_000382"
     CDS             complement(16707..17765)
                     /locus_tag="PXA33_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HDL5119752.1"
                     /translation="MAYPIKSYATFSATTPLAPHSFERRELLEDDVLIDILYCGVCHS
                     DLHMARNDWGWTQSYPLVPGHEIIGRIIKVGKNAKKFQIGDLVGVGCMVDSCRECSPC
                     QHGLEQYCEVGNIGTYGGIDRFDGTPTQGGYSTCVVVSEDFVLKVSENLDTKAVAPLL
                     CAGITTYSPLRHWNVKAGSKVAVVGLGGLGHMAVKLAKAMGAEVTLFTRSLGKADDAY
                     RLGASRVVLSTDEKQMADVANTFDLIIDTVPYTHDLKPYIPTLALDGTLVLVGLVGEL
                     EQTINTVPMILGRRSISASVIGGIQETQEMLDFCAEHNIVPDVEMIDMQNINDAYERM
                     LKSDVKYRFVIDMGSLKG"
     gene            complement(17952..18887)
                     /locus_tag="PXA33_000383"
     CDS             complement(17952..18887)
                     /locus_tag="PXA33_000383"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03571 family LLM class oxidoreductase"
                     /protein_id="HDL5119753.1"
                     /translation="MNTLSPIFSQVFGQNQLTLGLIAPFMGYQNHIPDNLTTIADNAR
                     LAENLGFSALWVRDVPFYDPHFGDVGQGLDPMVTLGFLSAHTQNIALGTAGLIAPLRS
                     PIHIAKMATSVDSLSHGRFLLGLSSGDRPVEYPAFGADFASRSERFRESIDLINTLTE
                     QDFPHFKGKHYADLTDNLDLLPKPKHRLPIVAIGRARQEMDWLANVPDAWIWHGVNPA
                     DTANIINTLADLRQKDTPTAFGYAQFVEVLDDKNAKAQLFNNIYLRGGAKGLADFWAK
                     EQEKGVKHLALNLKPTQRPADEVLTELAEYALTQF"
     gene            complement(19167..19478)
                     /locus_tag="PXA33_000384"
     CDS             complement(19167..19478)
                     /locus_tag="PXA33_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL5119754.1"
                     /translation="MSETEKQAINAPNAVMNGYLTMHYPDWFKPDGIYFNDGAFESFE
                     SSHKLTKDGKIRLVPTAGHTLGHLAVVVDMGEHYILIGGDASYSEQDMLAGNIDGVCN
                     A"
     gene            complement(19485..19877)
                     /locus_tag="PXA33_000385"
     CDS             complement(19485..19877)
                     /locus_tag="PXA33_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL5119755.1"
                     /translation="MIKVHCLTTGWVQIKIHHQVARFFARPARVLDVLTDKKWSPKLP
                     IGCWLIEHDDGLILVDTGESSRANDKGYQPWWHPFMQFCERRGVKKHEEVGEILKANG
                     FDPLRIDTVVMTHMHGDHAGGLPNFPNS"
     gene            20009..20569
                     /locus_tag="PXA33_000386"
     CDS             20009..20569
                     /locus_tag="PXA33_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HDL5119756.1"
                     /translation="MNDLRVIKTHLAIQNALIELLDEKLFEKVQVQEIIEKALINRTT
                     FYRYYSGESDLVGKLIKEVKAEYQDLLKKRFESGNLLRFMQTIGNRLLEKRRLILALW
                     QIKTPRHHLYDDMHMLLKNAFIAHAKRITSDDKNWAEKNWDYQAHIFATIALESHKYY
                     FEQGKLLPIPQVFEEWIEMVQVLKSV"
     gene            complement(20635..21624)
                     /locus_tag="PXA33_000387"
     CDS             complement(20635..21624)
                     /locus_tag="PXA33_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012073273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HDL5119757.1"
                     /translation="MSKQIQFTKTGSPDVLQIVDVAIPAPKAHEVQIQIQAIGLNRAE
                     MMYREGAYVIDPVFPATLGYEGAGVIVAIGEGVNEVAISDKVSIIPSFMFTEYGTYGE
                     IVNMPKHAVVKHPDNLSMEQASASWMAFVTAYGGLIEFGKVQKGDFVVLGGATSSVGL
                     ASIQIAKMQGATVIALSRTHAKGDVLLEKGADFVIATSEDDVTAKLFEITNGKGVNVV
                     FDPVGRKEAAKIINAMAQDGRYIIYGALSHDDIAVPVFPILGKHLTVRGYELFEITTV
                     PEKLAQAKTFVYDGLASGQLRPEIDKIFAFDEMVKAHEYMASNQQIGKIVVKV"
     gene            complement(21838..22755)
                     /locus_tag="PXA33_000388"
     CDS             complement(21838..22755)
                     /locus_tag="PXA33_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDL5119758.1"
                     /translation="MTFIIGILAGVLIAFQSAINARLRMHVGAAFLTSFVSFSVGLLF
                     LLGLSLIMQQTPAFSLDNLGNVPLWAWTGGLLGMVGLTANVLIFPKLGGVQTAVMPIL
                     GQVLMGVLIDTFAWLNSPYIAFTASRLFGIGLVLFGVWIAVVLPHLSGLKRKENANLW
                     LWRAVGILGGTALATQAAVNGEFGRQPGSSLGAATVSFGVGAIGLLLVVLFYEKSLPK
                     LCQPTEGKPFWIWSGGILGSLFILAGVWLVPQIGTGSVVMLVLSGLICGSLLVDKFGL
                     FGITQKPILPLQIIGVTLLLAGVAFIRLL"
     gene            complement(22891..23550)
                     /locus_tag="PXA33_000389"
     CDS             complement(22891..23550)
                     /locus_tag="PXA33_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003672168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDL5119759.1"
                     /translation="MQFFNKEDVINAFHYRASTRSYDGSRKIPAEDFNYILELGRLSP
                     SSVGSEPWQFLVLQNPELRQKIKPYCWGIPTMETSSHIVVILAKKNARYDSQYFSEIM
                     DRRGLQGEAREKAMAVYKKFQEKDIGILDSERSLFDWASKQTYIALGNMMTGAALIGV
                     DSCPIEGFAYQEVNDILAAEGLFDPSEWGVSVMCTFGYRDKEIRQKARKDFETVVKFI
                     E"
     gene            complement(23735..24799)
                     /locus_tag="PXA33_000390"
     CDS             complement(23735..24799)
                     /locus_tag="PXA33_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HDL5119760.1"
                     /translation="MAKFRKLFTPFNIKNLALKNRVVMPPMCQYSATNGVPNDWHFVH
                     YTSRAVGGVGLIIVEMTNVAPNGRITPNCLGLWNDEQQQAFKKLVDSVHAQGAKIGVQ
                     IAHAGRKAQDEPDAVAPSAIHYGELDYAGQNLITPRELTTDEVKALVQAFKDSVKRAV
                     EAGFDTIEIHGAHGYLIHQFYSPKSNQRSDEYGQDKMLFGEQVIQAAREVMPSEMPLI
                     VRISAQEYGENGFGLDYGLQVAKRFADAGADVLDISGGGDGVLHAGNHPEFYAGYQVD
                     FARQVKLATGKPVIAVGMLDNPAVGDHILGMGDADLVAVGRALLRDPYWVLNAQYDQN
                     AADSSEMQFVPKQYERGLFM"
     gene            24890..25798
                     /locus_tag="PXA33_000391"
     CDS             24890..25798
                     /locus_tag="PXA33_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL5119761.1"
                     /translation="MSDIRTLDFNLLKAFVVLLDECNVSRAAQRLSITQPAMSGILNR
                     LRESFNDPLFVRVQHGMQPTDRALQLGQTARNILQDISSMLQPPILEPEHLNMTLRIA
                     AMDYVQQIIALPLILRLRRLAPNVKVALLPVQGQNIKTLFEQKKIDLALVSRQHLSTD
                     TLKTVLYEERYVCAMSKTHPMAQTPLSLDQFCNLPFAMLSYNGGEFSGATDLALQKIG
                     LQRKVMVSVNHISLLPQLLQGSDLVAVLPERLAKTLPNVYLQPPPIEVEGFTMMMAWH
                     ERTEQDTAHRWLREVLQGVVKPKQAL"
     gene            complement(25868..26419)
                     /locus_tag="PXA33_000392"
     CDS             complement(25868..26419)
                     /locus_tag="PXA33_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDL5119762.1"
                     /translation="MAKQIAVLIGSGSKTSFSHLTVRHLQKMAPASIQLNIVDIADLP
                     LYDRDLDENSPAQYTRVREAVAVADGVLLVTPEHNASFSAMLKNAIDVVSRPMGQSKW
                     IGKPAGIVTVGASPAGGVRAADQVRTITSGSFVNMPTLPFALNVGNLFTGVFDEQGEI
                     ASETLKSSMQNFINAYAQFVEKY"
BASE COUNT         7447 a         5711 c         5691 g         7636 t
ORIGIN      
        1 tttttttagg gaaaatctgc aaatgctttt ttagctccat tcaagcgtat aaggcttaat
       61 accaatccct ttactattaa gacagtcttg tagatttcta atattattaa caatattttc
      121 ggtaattagg ttggcttgtt taactttttc ttcagtaaca ttgggatctt tttcgttgaa
      181 taaaatcgca tttaataatc tttctgcgtg cataccctct cggcagtaat gtaaaacacc
      241 gacatcatta aatgcagtag ctacagagct aatttgtgta gtggctatgc ctaaagcacc
      301 atcttctcca cctaattctt ggaagagctg atgttgtgga aagccgccac aagcgaggaa
      361 gaaactacgc cgaaatacag tgttgcctgc accagtcatt tgcatatatt gccaagctaa
      421 ctcaaaatta gggtgagagc tgtaacgttg atctaagtta ccggttttaa tgctaaccga
      481 accaagccgg tttccggttt aaactcaaaa atcttttcag caacttgcaa tgccccgttt
      541 tcataagcat catcagcatc taaaaaggca attaaatctg tttcactttg taatgctccc
      601 cagttgcgtg ctttggcaac accgccattt tgaggcattt gttccactct aattttatca
      661 ggcacttggg cttgtaaatg tttagctaag gcaagcgtat tatccgttga agcatcatca
      721 ataagccaca atagatttaa tccagactga tttaatacac tttgcactgc acgaacaagg
      781 gtgttttcgg cgttatagca aggaataatc acattaatca tataaattca gctccttgct
      841 cggttacgct taaaatagag gcgtaatttt ctttccagtc gcctaataca atgcgggtaa
      901 atccattatg ctgatggatt gcttgtcgat gcgtatgccc gtgtataagt tgagttgtat
      961 tgtattttgc gacaatttga gcggtaactt cagcgtttac gtccataatt tcagcggatt
     1021 tatcacgctt ttcttgtttg cttttggatc gaattttctc cgcaatacga attcgccaag
     1081 aaagcggaaa catcaaaaag atagcttgtc gccatttttg gtggactttt ttacgaaatt
     1141 gttgatattt cacatcatca gtacaaagcg tatcgccgtg gcaaagcaag gtagatgtgc
     1201 cgaataaatc tataacgtga taatcaggca aaataaccat acccgtttgt tcgctgaatc
     1261 gtttgccaat taaaaaatca cgatttccgc aaataaaata gcattttgta ccgcttgtag
     1321 tgagggtttt gattgcctgt ttaacggttg aaatgagttc agactgctca tcatcgccaa
     1381 tccagaaatc aaacagatca cctaagatat aaaccgcttc tgcaagtagt gctttttctt
     1441 gcataaattg caaaaagagg tgggtaattt caggctgatt ttcatttaag tggagatcgg
     1501 caataaaata gtacgtcatt tgatattgtt gataaaaaag aaaacggttc aattttttac
     1561 aaaatcgaac cgtttgtcaa aggttaatcg gcattaatct ttaaaaatgc cacgaataaa
     1621 ttgtactgag aggaagaaca agaacgccaa tacggcataa agtacatagc tcataattgg
     1681 tttgctgact gtttctgctt cttctaatgg cttgattgaa acaatatttc taaattcatt
     1741 tagccaattg attcgccagc cgtaatattt aatttgcaca cgttgttttt cgttagcgta
     1801 ggcttgagct ttggcttgaa tgtcggcaga tccgaatttg aaataaggag gcaaccccca
     1861 gccggtatct tcattacgat aaaccattac ctttttggtt tcaggatctt ccgtgaataa
     1921 gaaataaaca tcgcgcacct cgccatcagc aggattcgct ttgctgataa taccatcttt
     1981 atccattcgg cgtacttcca tacctgttac tacggtatct tcataacttg gcattgcata
     2041 attaactgca ccgcctaata caataaaaaa tgatagtgag acaagaatca gaaaatactt
     2101 aagtattgct ctcatattag ttccttataa tttaatgttg aataagtgtt gcacattttc
     2161 ggttgtaatg cgagctaatt cttcagttga aacgcctttt aatgtcgcca catattcgca
     2221 agtttcccgc acatacgccg gctggttcgg cttaccacga taaggaatcg gggcaagata
     2281 aggggaatct gtttcaacca ataagcgatc taacggcact tttcgcacga cttctcggag
     2341 ttcttctgca ttgcggaaag taataatgcc cgaaattgag atataaaagc cgatgtctaa
     2401 agcccgtttt gccattgccc aatcttcggt aaagcaatgc aacaccccac cgcatttttc
     2461 ggcattattt tgttccaaca tatccatcgt atcttggcgt gccgaacgag aatggataat
     2521 gagtggctta ttgagtttgt ttgcaatttc aatttgttga acgaaaagcg attgttgcaa
     2581 cgctttggtt tccggtgtgt agtgataatc caaaccagtt tccccaatgg caaccacttt
     2641 cggatcttga gcgaactgaa ataatttatc gtaatcaaaa ggttcatctt ccacattcaa
     2701 cgggtgaacg ccacaagaaa gtgaaacgtc attacgatgg gcggttaagt ttttcattgc
     2761 ttcaaaacgc ccaactgtag tgcaaaccga gataaaatga tgcacccctc gggctttggc
     2821 attttcaatt acttcgtcca cgtttttgtg acgagtttcg tagtctagag aatctagatg
     2881 acaatgtgag tctatgataa ataagtcttt catttttcta aatatagcgt ttatgattca
     2941 tttggttatg taagatgcga acaataaata tatgatctgc ttttattcga taataaattg
     3001 tatgagattg atgtgtgtgc attcgcaagc caccacgaat atattccgca gaagttccca
     3061 ttgttggaaa atcagctaat aaattcaaaa tatgctttag atctgcaaaa taagattctg
     3121 tttgttgaac accaaattga gagatactgt aacgaaaaag tttctcttga tctcggagtg
     3181 cttgagcaga aagtttatac attaagttcc gcctttactc tcaacatcat ttcatcaatc
     3241 gtttcggttg cataaccact agcatcacca tcatcaatgg cttgtcgcaa ctgagctaag
     3301 tattgctctt tttcctcaag cattcgtaaa gcggttctca ctacttcact ggtagagcca
     3361 taacgcccac ttgccaccat ttcatttaca aaatcattta aaggttcgcc taaagtcacg
     3421 ctggttgttc ttgccataat attctccttg tgtgaattat tcatacaata acaaagatag
     3481 ataattttgc aagttataaa ctatattcta gggctagaag cctcttttta cggctaattg
     3541 aaagctacct tctatgagag agggctttta ctcaaacacc aaaaccaact tcgtcaggca
     3601 atctagcaac atcaattccg gattgacggc gttaatttcg gttaaatccc gctgggtttg
     3661 ttggagaatt tgatgccctt taagtaagcc gtgggtcgtt agttgttggc taaaagggag
     3721 aatgccgttt tggagatccg ggtttagcca accgtggctg attgccattt tagcttttag
     3781 ggcatcgcta aagaaacttt ccagccattc gagttgctgt aaaatcagct ctttttcttg
     3841 ggcgaaggct tggaataaca agaagacatc gtggcttttg tagaaacgcc agaaggtttg
     3901 caaaaaggtt tttcgctgtt gcaagcggtc attttcgata aaatttttgc aaattagtgg
     3961 gcgtgagtgg caaaggcgga gtgcaatctc taagtcgtcg gttgagattg ccggaaattg
     4021 tgtttgtagc caagccatta cttctgaggc ttgtggggcg taaagtaccc aagtttggct
     4081 gcggctttga atggtagcta aaactgttgc ttgaagcggt gcttcgagca agaaatagac
     4141 gttttcacgt ggctcttcca aggttttgag cagggcatta ctgcttgcct cggttaaacg
     4201 atcaactcct cgaatataaa cgaccgtatt tcccccttgt tgggcgaaat tctggagttt
     4261 ataaatcaac tcacgcactt ggtcgatacc aatatctttg tcttcaatcg gctcaagaat
     4321 gtggaaatcg gggtggttgt tgctttgcat aagcaggcaa cttttgcatt caagacaagg
     4381 ttgatggcgg agtttttggc tgcaaagcaa ccattcagcc accttgtaga ttaaggtgtc
     4441 tgtaccaaga ccaacatctg ttttgaacag taaggcgtga tgacctcgac cctgtaaaaa
     4501 tgaatcggta agttgttgat aagtgctttc gagccaaggg tacattattt cagccagttt
     4561 ctaattactt gttgaatatc cgcagtcacc tgttcgatgg gttgttcggc attgatgatc
     4621 acggctttat cgttgtgttg ggttaattct aaataacgct ctcgtgtgcg gtagaagaag
     4681 tcaaggcttt gctgttcgat gcggtctaat tcgcctctgc ctctggctct ttctaaacca
     4741 atcaccggat cgagatcgag ataaatcgtt aaatctggct cgaatgtgcc gagtaccgat
     4801 tcttttaagg tttcgagtaa gtgacgatcc aacttacgtc caccgccttg ataggcttgg
     4861 gaagacatat cgtggcggtc gcccagaacc catttgcctt cggcaagggc aggtttgatc
     4921 acattttcga ctaattgaac acgagcagcg tagagcatta gcagctcagc cttatcgtta
     4981 atggcttctt cggtttcgtg tttaattaaa tggcgtaact tttcggcaag gggggtgccg
     5041 ccgggttctc gggtcattac tacgtcatta atgccggctt gttccaactc tttgagaatc
     5101 acttggtgag cagtcgtttt gcctgcgccc tctaagcctt caattacaat aaattttcca
     5161 cgcatattgt tctcactatt tttgttcatt tttgcgtttg cgttcaattt gaatccattc
     5221 tgccaccgct ttattatgct cattcagtgt tttgctgaat ttatgaccgc ccgtgccgtc
     5281 tgccacaaaa tagaggtagg gtgtattatc cgggttggca accgctttaa tcgaagcttc
     5341 actcggcatg gcaatcggcg ttggcggtaa gccgtcaatc tggtaagtat tgtatggcgt
     5401 tgcctcttct aaatctttgc ggcggatatt gccgttataa cgatcgccca ttccgtaaat
     5461 gacggttgga tcggtttgca gtcgcatatt gttgcgtaaa cggttaataa agaccgatgc
     5521 cacttgtggg cgttcggtcg caatgccggt ctctttttcc acaatcgagg caagaatcag
     5581 catttcgtat ggtgttgcaa gcggtagatt ttccgctcta tttttccaag cctgttccaa
     5641 cgccgttttc tgcttttgat aagcccgttt aagcaaggct aaatcggtgg aattgggcgt
     5701 gtagttgtaa gtatccgggg ctatccaacc ttcgattttt tcgtgtggaa tgccgagtaa
     5761 gtcagcgatt tctttttccg atttgccttg tagcgtgtgc tgtaaataat cggctttcgc
     5821 tagttgttcc cgccaaattt taaaagtttt gccctcaata aattgcacgt taagttgcac
     5881 ttctttgccg gagcttaaat gagcgagcaa atcttttact gtcgttaagc ctgcgagtga
     5941 ataagcacca gccttgaatt tgcttaactc agggtataat cgcattaaat aagggataag
     6001 atcggcgtca tcgtgggtga caatgccttg ctcttctaac agagccgcta atttttggct
     6061 ggaaacgcct ttttccaata cgaaaagttg attcggctga accgtgatcg ggtgttcggc
     6121 taaagaggtg agtttttggt agccgtaaaa cagtccaccg caagcaataa cgccggctaa
     6181 gcctaatgca ataaggattt tttttagcat tgatgaataa aataaaagtg ataaaaattg
     6241 actgaattct actataaagc ggttgttttt tcagaaaaat ttgcaaatgg gatttgagta
     6301 aatggtgggt catgaaggat tcgaaccttc gaccaacgga ttaaaagtcc gctgctctac
     6361 cgactgagct aatgaccctc agaggagttt tgaaattaat ggtgggtcat gaaggattcg
     6421 aaccttcgac caacggatta aaagtccgct gctctaccga ctgagctaat gacccttaga
     6481 ggtgttttga agattaatgg tgggtcatga aggattcgaa ccttcgacca acggattaaa
     6541 agtccgctgc tctaccggct gagctaatga cccataatct tgcgtaacga gggcatatct
     6601 tacggatttt taaaactttg gcaagttttt tttatcgaaa aatggttgag tgtgcggttt
     6661 gtaagcaatt tagcaaaaaa ttagccggat ttgttggcgg aaaatgaaaa gagaaacggg
     6721 cttttatctt tttctgaaaa taggctataa tcacgccgtt tatttcacct attatagaag
     6781 gaaaccaaaa tgggcttaga attagttcct gccggtaaag aattaccgga cgatatttat
     6841 gttgtaattg aaattccggc aaactctgat ccaatcaaat atgaagtgga caaagaaacc
     6901 ggggcattat ttgtggatcg ctttatggca accgcaatgt tctatccggc aaactatggt
     6961 tatgtgaata acaccttatc atcagacggt gacccggttg atgtattagt gccaacacca
     7021 tatccgttac aaccgggttc ggtgattcgt tgccgtcctg tcggtgtgtt aaaaatgacc
     7081 gatgaagccg gcggtgatgc aaaagtggtt gctgttccac acaccaaatt aagtaaagag
     7141 tatgaccaca tcaaagatgt aggtgactta ccggcgttat taaaagcaca aatccagcac
     7201 ttcttcgaaa gctataaagc attagaagcg ggcaaatggg tgaaagtaga aggttggggc
     7261 gatgtaaacg aagctcgcca agaaatctta gaatcattcg agcgtgctaa gaaataatta
     7321 tttatttttg aatgccaagc aaatttgttt ggcatttttt gtgggatata aaatgaaatt
     7381 catcaaaaaa ttaagttatt cagttttttt agcaacagtg attaccgctt gtgccacaac
     7441 agaaagcatt aaccaagaag cggctcaaag ttatgctcaa gtgaaaaccc aagctcgtca
     7501 gcaaaatgcg attgacaatt cgtctgccac cgctaagcgg attcacactg tttttcataa
     7561 aatgaagcct tatgcggaaa gagccaacca gactggtgta ccttttcatt gggaaattac
     7621 cgtattgaaa tcggatgaac tcaatgcgtg ggcgatgccg ggcggtaaaa tggcgtttta
     7681 cacggggtta gtggaaagac ttaatttatc gaacgatgaa attgcagtgg taatgggaca
     7741 cgagatggct cacgcactga aagaacacgg taaatcagac cgtacggtta gcgctattac
     7801 cagtattgta ggtgctattg cagatgtggc tgtgactgcc tcaacaggcg taagcaccga
     7861 aggtttactc agcactggcg tggatttgat tgccaccaag ccattctctc gcagccaaga
     7921 gactgaggcg gacgaagttg ggttaatttt aatggcagaa tcaggctaca acccatcagc
     7981 agcgccgaat gtttgggtaa aaatgagtaa agccaatggc gattcaggct tgtccatttt
     8041 ctcaactcac ccatcaaatg ctgaccgtca agaaaactta gcccgccttg tgccggaagc
     8101 aatgaagatt tataacgccc gtaaataatt gacaatcggt tcttttttgc aaaaatgctc
     8161 ttgaatcctc aagagcattt ttcattaagt gttagtaacg cacaaaaccg actaattcat
     8221 ataccttatc taacgttgct ttggcttgag ctcgagcttt ttctgcccct tcacggtaga
     8281 ttttttccaa taatgcttca tcttggcggt agtggtgata acgttcttgc agattggtta
     8341 atagttcgga aaccttatct gctacttctg ttttcaaatg accatacatt ttgccttcaa
     8401 attcggtttc taattcggca atggttttgc cggtaatggc ggagagaata tccaataaat
     8461 tcgagacacc ggctttattc tgtacgtcat aacgcactac tggcggttca tcgccatcgg
     8521 tcattgcccg tttgattttc ttcgctaccg ctttcggatc ttccaatagt ccaatcacat
     8581 tgttacgatt atcgtccgat tttgacattt ttttggtcgg ctcaagtagc gacatcactc
     8641 ttgccccgga tttcgcaata aagacttccg gcactgcaaa aacaggctcg atcaggttgc
     8701 cttcggaatc ttttttgccg tataaggcat tgaaacggtt ggcaatgtca cgggtgattt
     8761 ccaaatgttg tttttggtca tcgcctaccg gcacttggtt ggcttggtaa agcagaatat
     8821 ctgccgccat taataccgga taagtaaata agcccacatt gacattctct tcatagcgtg
     8881 ctgatttatc tttaaattgc gtcatacgcc ccatttcacc gaaataggtg taacagttca
     8941 gcacccaagc taattgggcg tgttccggca cgtgggattg aataaaaata gtgcttttgt
     9001 ttggatcaat gccacaagct aaatacaggg ctaacacatc taaagttgat ttgcgtagcg
     9061 actccggatc ttgacgaacg gtaatggcgt gttgatcgac aatgcaatat acacagtcat
     9121 aatcatcttg cattttcacc cagttgcgta acgcccctaa gtagttaccg atagtcaatt
     9181 cacctgaggg ctgcacgcca ctaaatacaa taggtttgct cattttatct tctcgtttga
     9241 tttgaaaaat ataggttatc ttaacggtta ttgggaaggg ataaaagtac aagcggtcag
     9301 atttattgat gattttgcaa aaaatagagc aaatcagacc gcttgtatct tactcaaaaa
     9361 tcactgaaac ccttgccata atttcatcaa gtaggctttc gggtaaggct tcgataaatt
     9421 ttccatggcg ttgtgcaata tcgactgtgc gaggttgatc gcatcgaata atccctgttg
     9481 tttttaagcc ggcatctttt aatgaaacgg caaagcccag tctttcagca aaagctccac
     9541 cgttagtaat cggcacaacc acaggcagtt tactggctag attaaatgca gttgcggaaa
     9601 caattacaac ggggcggaag cctgattgtt cgtgtcctaa ggttggatca agatcgagcc
     9661 agtaaatatc gccacgtttc ataaaatttc ttttcctatt gccggctcgg ataaccacga
     9721 ttggctttct tcattccttg ctgcctcaaa atcggattgt cttaataatt cctctaagct
     9781 gtatttcact tttgggcggg caggattaat cacgagttgg ttgtcgttta aagacaaatc
     9841 cactttgcta ccgacggaga gcttgagtgc ctctaaaaaa gcaggcggaa tcaccattgc
     9901 aaccgagcca ccgacctgac gtaatgcgat tgttggcata ctttactcct ttaaatcttt
     9961 aggttcggaa agtctaaagc aaacatcaaa agaaaacaaa tgttttcttt ataaccccac
    10021 aattttcaac acatctttaa attcagatgt cacaaaagtc gggttagatt gttcaatcgg
    10081 cacattgtag ttatagccgt aagttagtcc aaccacatca caaccggcgg ctttggcggc
    10141 aatcacgtca ttttctgagt cgccgacaaa aagtaattct gatggggtaa tgccgaattt
    10201 ttcgcagata aaaaacatcg ggtcagggtg cggtttgatt ttggggagcg attgcccacc
    10261 aagggtttca tcgaataaat gataaatgcc gaaagcggaa agcaccggct cgactaattt
    10321 ggtcggtttg ttggtaatca ccaccagcgg gtagtcgttt gctttgagga tttctaaggt
    10381 ttctttcaca tttgggaaaa gggtgctttc ctcgcacaca taggtggcgt agtatttatc
    10441 aaagctcact cgcattttca ccaactcatc ggcttggaaa attctgccgg tgtgagcaac
    10501 cgcattttgg aagaaaatat ctgccccttt accgatccaa gtcagcactt tttcttgtgt
    10561 aaccgttggc aaaccggctt ctagaaacat tgaattggcg acaagagtga gatcggggag
    10621 ggtgttgatt agcgttccgt ctaagtcgaa accgatgact ttgtattttt tactcatagt
    10681 atatccttca aacacaagcg gtcgttttct gcaaattttt tgcaattttt gtaatgaggg
    10741 caaatattga cttgccccta cgtttatttc actttgttta ctttatacgc ccacgccata
    10801 atcagtatgc cgccgataat catcggtaaa gagagtagtt gcccacgagt gattaaatct
    10861 gcggtggtat tgacgttatg gtcgatttct cggaagtatt ccacaataaa gcggaatgtg
    10921 ccgtagccga ttaaaaataa ccctgaaacc gcacctgccg ggcgaggctt ttgaataaac
    10981 cagttgagga taaagagtaa caccacaccc tctaaaaagg cttcataaag ctgtgacggg
    11041 tggcggggaa gatagccacc gcttggaaat aacaccgccc aaggcacatc ggttacacgc
    11101 ccccaaagtt catcgttaat gaaattgccg atacgcccca tacctagccc gaacggtact
    11161 agcggtgcga taaaatctgc tgtctgccag aaattgcgtt tttggcggta ggaaacccaa
    11221 atcattgcta caatgacgcc gattaaaccg ccgtgaaagg acattccgcc ttcccaaatg
    11281 cgaaagaggt agagcggatc ttgtaagaaa cggtcgaagc tataaaagaa cacatcgccg
    11341 atacgcccac ctaatactac gccccagaag caagtataga ctaagctatc cacttgctcg
    11401 gttgtccaaa ctccattgga atttttcgcc cgtctcattc ccagccagta ggcaaaggca
    11461 aagccggcta aatacattag cccataccaa cgcagggcaa tcggaccgat ttcaaacaca
    11521 atcgggctaa tttgtggaaa ttggataaat tgttcgttca tagtgtcctt attttaaaaa
    11581 catattgatg gcgatacaga ccaaaagcac cgcaaaggct tttttcagca tcggcacagg
    11641 tagagcgttg gcggcacttg cccccatttt tgaggtgaaa aatgaagttg ccgtaatacc
    11701 aaacagagcc ggtaaataaa cataaccgag agaataatca ggcattcctt ctaagcccca
    11761 gccacttaca ataaagctga ttgtgccaga taaacctaaa aatgctccgc aaaatgagga
    11821 agtgccgatg gcacgtttca tttctaagcc tcgatcgttt aaaaatggta caataaaggc
    11881 tccacctgcg atccctgcca tactggctaa cgcaccgatg attgaacctc cgattatcgt
    11941 cgtttgaacg gtaagtggtt tatttttcgg tgctcttttt attgaaaaaa tcattttacc
    12001 tgcaaggtaa agtaccatta ccgcaaaaat tctagacatc agttgcggat cgaggcggct
    12061 taccaccaag cccgatagaa aaaccgaaac catcaacgct ggaataaaat atttactggt
    12121 ttggatatcg acattgccta acttatgatg gcgttgtgcc gatgcaaagg cggtaataat
    12181 aatggttgaa aatgatgtgc cgagtgcggt ggacattata tattccggcg gtacacctgc
    12241 catgggcagt aggaagacca aactcggcac gatgattaac ccaccaccaa tgccgaacaa
    12301 gccggctaaa aagccgacaa acacccctaa aaaaaggcag cttaataaaa tagtcatctt
    12361 attttccttt ttgattatgt tgatgccaag gacgataagg cttacgagct tctcgtttat
    12421 aggctttggc agaatcctga gaggtttttt tataagtatg gggcgaagtc tcgccacgct
    12481 caatgcgtgg tttctcaccg gttttatcgt cagtcgcttt ttgaatattg ttttgcaaaa
    12541 cctgtgcaaa ctctttcatc gctttgcgat aaacatctcg tttaaaagag accacttggc
    12601 ggactggata ccaaaagctg acccaacgcc agccgtcaaa ctccggattt ttcgtggttt
    12661 tcaaattaat caggctttca tcaccgatta attgcagtag aaaccaacgc tgtttttgcc
    12721 cgatacagac aggaccagag ccttcattac gtaccaagcg ttttggcagt ttatatttta
    12781 gccaatattt agacgcccaa agcaatcgta catctttttt acttaagccg acctcttcat
    12841 aaagctcacg gtacattgct gcttcaatgt tttccccctc attaattcca ccttgcggaa
    12901 attgccaaga gttttgacca aaccgttttg cccacagaac ttgcccgtgc ttattacaga
    12961 taacgattcc aacatttggg cggtagccat cgaaatcgat cactcgacca cctaattgat
    13021 aaaaattcta gaatgggaga attgtttcat actttgggtt gatttgcaaa aaaaccataa
    13081 aatataaccg cttgtgaata actttgtgga taactattga atataagtgg tataagttga
    13141 ggataaacag gcggaacgtt gtaaaacaga gcaattaatc agatctaaaa atgcgatctt
    13201 tttcttaagc aatgatctgg ataaagatca agttgttgcg ttttttctcg tcaaaataag
    13261 tttaaatttt aaaatgaata agcagggcaa tttttgcttc gcaaataaag cagagttatc
    13321 cccaccattt gtggataaag atgtgatagt ttttcaaatt aggcgtgaat aactcaaatc
    13381 agccttttgc tatttccata aattataatt aaatcaaata gttagtgtca tcttttcaat
    13441 ctgtgaataa tcaattgttt tgttggttgc tttttgtgca attgtatttt tatgctaatt
    13501 ggtttgtaac aaaaatgtga tctatttctc atattttgca aatagtagta attcttattt
    13561 ttattttaaa gtagaatagc cgcacttttt tcttaatgat ttatgaggtt ttaaatgatc
    13621 atttcatctg caaacgatta ccgccgtgcg gcaaaacgtc gtgttccccc ttttatgttc
    13681 cattatgcgg atggcggttc ttacgccgag cagaccttac gtcgtaatgt gagcgatttg
    13741 gaagacatcg ccctgcgtca acgtgtgtta aaagatatgt cgcaactaga caccggcatc
    13801 gagctgttcg gcgaaaaact gtctatgccg gtgaccctcg cccctgtcgg tgcattagga
    13861 atgtatgccc gccgtggcga agtgcaagcg gccaaagccg cagataacaa aggtattccg
    13921 tttacccttt ccactgtctc tatttgcccg attgaagagg ttgcaccggc aatcaaacgc
    13981 ccgatgtggt ttcagctcta tgtgttaaaa gatcggggct ttatgaaaaa tgcattggaa
    14041 cgagccaaag ccgccggttg ttccacgcta gtgtttaccg tagatatgcc gacccccggc
    14101 gcacgttatc gtgatatgca ctccggaatg agcggcccat acaaagacat tcgccgtgtg
    14161 ttgcaggcaa tggttcaccc gttttgggcg tgggacgtgg gcattcacgg taaaccccac
    14221 acgttaggca atgtgtcaaa ctatatgggt aaaccgattg atctgaacaa ctacatcggc
    14281 tggctgaccg ataatttcga cccatcaatt tcgtggaaag atttagagtg gattcgtgaa
    14341 ttttgggacg gcccgatggt gatcaaaggc attctcgacc cagaagatgc taaagatgcc
    14401 gtaagatttg gtgcggacgg tatcatcgtc tccaaccacg gtggtcgtca gttagatggg
    14461 gtactttctt ctgccaaagc cctgccaagc attgccgatg cagtaaaagg ggagatcaaa
    14521 atccttgccg actctggtat ccgcaacggc ttagatgtgg tgagaatgct ggctctcggc
    14581 gcagattgta cgatgatcgg gcgttccttt gtttacgctc ttagcgccgc cggtcaagcc
    14641 ggtgtggaaa acctgctcga catcttcctt aaagaaatga aagtggcgat gacacttacc
    14701 agcaatgcta aaattagcga catcggcaga gatgctttgg tagatttaag taatctttag
    14761 ctcaaatagc cacaatccgc acgttttagg tgcggattgt tattttagat aggctaattt
    14821 ggcattttgg taatattaat gcggtaatat ttcaatgtat cttggttgaa aaagagaaat
    14881 tcatcgttat ctttagtttt ttcgcccagc acataaatgt tattgcgatg aatagataag
    14941 acataatatc gcttattttc aatttcaaga taatcatacg ttgttctcag ctcagggcga
    15001 atgtagccaa tactcaaaga aagtaagcta aaatagatga aaataaaaat atagaaaaaa
    15061 ccgaagcgtt cttcgcttaa cattttttga atatctaagt ggaatacgcg gtggccccaa
    15121 tgtttgtgaa ataataatac tgatagggta gttgtcccta gatagaggta gttgtcccta
    15181 gatagagtag gtgtaaatac attggcactt tgccaataaa cagatagaaa ctgatcatta
    15241 ctggcaagct aaaaatagta acaagaacac tcacccgtag ccagccaagg tatttaaagt
    15301 aatgcagctt aaaaacttta ttaactaaag cgtatcctat caaataaaaa agaaagatag
    15361 ttaaaaatgt gccaaaaacg taggctagtg cgcgagctat accggcatta ccaacctcaa
    15421 catgccacca cgggtagcca tgaaaagcag caatacccca agcataacag taagcggtag
    15481 aaaaagagat taaagtaaag aaagcgagag taagagaaga gttgatgaat ttctgattag
    15541 tcataacagt aaatagattg cacctactta gttaaagatg aaacacaaga aagcagactg
    15601 agtttagctt ccacccaact cctttgttta gaggagaact ttattaaagc acgacttttg
    15661 ttgtatttca aacctttgtt tttatacaaa acaaccgtgt tgtaaaaaat gatcgtaaac
    15721 tgaccgcttg taatgcactc tgaatatatc gaaacatgat aaaaatgata aagcatgttt
    15781 cgataattta ctgtctttag tcttttaaat gttttgcatg aaaggctata tgttcaccga
    15841 taaagctact aatgaaatag tagctgtgat cgtaaccatc acgtaaattg aattggtagg
    15901 gtattccaag tctatctgcg gtttcacaga aaatttccgg ctttaactga tcgctcaaaa
    15961 agctgtcttg gctgccttga tcgattagaa ttggcaagct tgggcgatat ttttccagca
    16021 atgccacgct atcatacgcc ttccaagcgg tctgatcttc gcctaaatat gctaaaaatg
    16081 ctttttgccc ccaaggaact tgggttgggg cgacaatcgg tgcaaatgcc gatacacttt
    16141 gatattgccc cggattttta aatgcaatgg ttaaggcacc atgtccgccc attgaatgcc
    16201 caaaaattga ttgtttaccg ttagtcggga aatgagcatt aatcaatgtt ggcagttcgc
    16261 tgacaatgta atcatacatt tggtaatgtg ttgtccattc gccttgcgta gcatttaggt
    16321 aaaatccggc accctgacct aaatcataag cctcatcatt agcgacattc acacccctag
    16381 ggctggtatc cggtgcgacc acaatcagat gatgttcgtc ggcataacgt tgaaagccag
    16441 atttagtgat gaagttctgc tcggtacagg ttaaaccaga aagccaatat agtacgggta
    16501 gcttttgtgt tttagcagat tgcggcagat aaatagcaaa tttcatttca cagtttaagc
    16561 tggctgagaa atgttgccaa acctgttgtt caccgctaaa acagcggtag ttttcaaatt
    16621 gttgcataat attccttaca agcggtttaa ttccgctgat tttttgcaaa aataaaggcg
    16681 gaactcaccg cctttatatt gtaatatcaa cctttcaaac ttcccatatc aatcacaaac
    16741 cgatatttca cgtctgattt cagcattcgt tcataagcat cattgatgtt ttgcatatca
    16801 atcatttcca catccggcac aatgttgtgt tcggcgcaga agtcgagcat ttcttgagtt
    16861 tcctgaatgc cgccgatgac agaggcggaa atagagcggc ggcctaaaat catcggtacg
    16921 gtgttgatgg tttgctccaa ctcaccgacc aagcccacca gcaccaatgt gccgtccaac
    16981 gctaaggttg ggatataagg tttcaagtcg tgggtataag gcacggtatc gatgattaaa
    17041 tcaaaggtat ttgcaacgtc tgccatttgc ttttcatcgg tagaaagcac cacacggctt
    17101 gcgcccaaac ggtaagcatc atcggcttta cccagtgagc gagtaaaaag cgtaacttcc
    17161 gctcccattg ctttggcaag tttaaccgcc atatgcccca gtccgccaag ccccacaaca
    17221 gcgacttttg agccagcttt gacattccaa tgacgcaggg gcgaataggt ggtaatgcct
    17281 gcacacaaaa gaggggcaac cgctttggtg tccaagtttt cagagacttt taatacaaaa
    17341 tcttcactca ccaccacgca tgtgctgtaa ccgccttgtg ttggtgtgcc gtcaaagcgg
    17401 tcaataccgc cataagtgcc gatgttgccc acttcgcagt actgctccaa accgtgctgg
    17461 caaggactgc attcacggca ggaatccacc atacaaccga caccgaccaa atcgccgatt
    17521 tggaattttt tggcgttttt accgactttg ataatacgac cgatgatttc gtgccctggt
    17581 acaagcggat agctttgcgt ccagccccaa tcattgcgag ccatatgcaa gtctgaatgg
    17641 cacacgccac agtacaaaat atcaatcaaa acatcgtctt caagcaattc acggcgttca
    17701 aaactgtgtg gggcaagtgg tgtggttgcc gaaaaagtgg cgtaggattt gatagggtaa
    17761 gccatttttg gggtttaaat ggctgaaatg gcaattacta tagtgccgat gatttattat
    17821 tgaaaatcaa gatttttgat aaatcggtat cagttaaaat gataattggg ggttttcaga
    17881 ctgactgaaa aattattttg cccattttaa aaacaaaaac accgccctca tcacaaaagc
    17941 ggtgtttttc atcaaaattg tgtcaaagca tactctgcca attctgtaag cacttcatca
    18001 gcaggacgct gagtgggttt taaattgagt gccaaatgtt tcacgccttt ttcttgttct
    18061 tttgcccaaa agtctgccaa gccttttgca ccaccacgca gataaatgtt gttaaataac
    18121 tgtgctttgg cgtttttgtc gtccaaaact tccacaaatt gagcatagcc aaatgctgtg
    18181 ggggtgtctt tttggcgtaa atcggcaagg gtgttgatga tgtttgcggt gtcggcagga
    18241 ttcacaccgt gccaaatcca cgcatcaggt acattagcaa gccagtccat ttcttgcctt
    18301 gctcgtccaa tcgccacaat gggcaagcgg tgtttgggtt tggggagcaa atccaaattg
    18361 tctgttaaat cggcataatg tttgcctttg aaatggggaa aatcctgctc ggttaaggtg
    18421 ttaatcaaat caatgctttc acgaaaacgc tcgctacgag aggcaaaatc cgccccaaat
    18481 gcagggtatt ccacaggtct atcgccagag gataagccca ataaaaaccg tccatgactc
    18541 aaactatcca cacttgtcgc cattttggca atgtgaatgg gactacgcaa aggggcaatc
    18601 agccctgccg tgccaagggc gatgttttgg gtgtgggcgg acaaaaaacc aagtgtaacc
    18661 atcgggtcaa gcccttgccc cacatcgcca aaatgagggt cataaaacgg cacatctcgt
    18721 acccacaatg ctgaaaagcc caagttttca gccagccgtg cattgtcggc aatggtggtc
    18781 aaattgtctg gaatgtgatt ttgatagccc ataaaggggg caattaagcc cagtgttaat
    18841 tgattttgcc caaaaacttg ggagaaaatg ggggaaagcg tgttcatttt ggcgtacctg
    18901 tcttatttaa gataaggtat tttaagggct tttgagtttg cttaaaatgg gataaattga
    18961 gaaagtgttt tgctaaattg gcataaatag acgctttcaa gctgactgaa aatttgcaaa
    19021 atttttaata aaaatgaccg cttgcctttc aacaagcggt cattcccctt taatttttta
    19081 ccaccgtaaa caccttattt ttcagccgat attcactttt ttcatcgtga gccagttgca
    19141 caatggttgg cttgcgttta cacaattcac gcattgcaca cgccgtcaat gttgccagca
    19201 agcatatctt gttcagagta agaggcatcg ccgccaatca aaatataatg ctcgcccata
    19261 tccaccacca ccgccaaatg ccccagcgta tgtcctgcgg ttggcacaag gcggattttg
    19321 ccgtctttgg tgagtttatg actgctctca aaactttcaa acgccccatc attaaaatag
    19381 atgccatcag gcttaaacca atcaggatag tgcattgtta aataaccgtt catcaccgca
    19441 ttgggggcgt tgattgcctg tttttcagtc tcactcatca caaatcagct gtttgggaaa
    19501 ttgggcaatc cgcctgcgtg gtcgccgtgc atatgggtca ttaccaccgt gtcaatgcgt
    19561 aaagggtcaa agccgtttgc ctttaaaatt tcgcccactt cttcgtgttt tttgacccca
    19621 cgccgttcgc aaaactgcat aaacgggtgc caccacggct gatagccctt gtcgttggca
    19681 cgactgcttt cgcccgtatc caccaaaatc aaaccgtcat cgtgttcaat cagccagcaa
    19741 ccaatcggca gtttaggcga ccatttttta tcggtcagca catcaagcac acgggcagga
    19801 cgggcaaaaa agcgtgccac ttgatgatga attttgattt gtacccaacc tgttgtcaga
    19861 caatgcactt taatcatttt tgtgttctca ttaaaaaatc gtgggcatat tgtagaaagt
    19921 gcggtaactt ttcacaaaca ttaaaaagtt cgtatttggc tggatatgca gataaaatta
    19981 caaaaatgta tgcttatttt gaaaaaagat gaacgattta agagtaatca aaacccatct
    20041 agcaatccaa aacgctttga ttgaattatt agatgaaaag ctgtttgaaa aagtgcaagt
    20101 ccaagaaatc attgaaaaag ccttgattaa ccgcaccact ttttaccgtt attattcggg
    20161 cgaaagtgat ttggtcggca aattgataaa agaggtgaag gcggaatatc aagacctgct
    20221 caaaaagcgt tttgaaagcg gtaatttgct gcgatttatg caaaccattg gcaaccgctt
    20281 attggaaaaa agacggctga ttttggcact ttggcaaatc aaaacccctc gccatcattt
    20341 gtatgacgat atgcacatgc tgttaaaaaa cgcctttatc gcccacgcca aacggataac
    20401 atcagatgat aaaaactggg ctgagaaaaa ttgggattac caagcccata tttttgccac
    20461 cattgccctt gaaagccata aatattattt tgaacaaggc aaacttttgc ccattccaca
    20521 ggtgtttgag gagtggatag aaatggtgca ggtgttgaaa agtgtttaga aataactggt
    20581 aaaattttcc ataaaaaaca ccgctttcat cacaaaagcg gtgctttcaa atcatcaaac
    20641 cttaaccaca atcttaccaa tctgctgatt gcttgccata tattcgtgag ctttaaccat
    20701 ttcatcaaag gcaaagattt tgtcaatttc agggcgtaac tgaccgcttg ccaaaccgtc
    20761 atagacaaag gttttggctt gtgccaattt ttcaggtacg gtggtaattt caaagagttc
    20821 atacccccgc acggtcaaat gcttacccaa aatcgggaac actggcacag caatatcatc
    20881 atggcttaac gctccataaa tgatgtaacg accatcttgt gccattgcat tgatgatttt
    20941 tgccgcctct tttctgccca cagggtcaaa taccacattg acgcctttgc cattggtgat
    21001 ttcaaaaagt ttggcggtta catcgtcttc gcttgtggca atcacaaaat ccgctccttt
    21061 ttccaaaagt acatcgcctt tggcgtgggt gcgagacaag gcaatcacgg ttgccccttg
    21121 cattttggcg atttggatac tggctaaacc aaccgaactg gttgcaccgc ccagcaccac
    21181 aaaatcgcct ttttgtactt tgccaaactc aatcagtccg ccataagcgg taacaaacgc
    21241 catccaactg gcggacgctt gttccattga caaattatca gggtgcttga ccacagcgtg
    21301 ttttggcata ttgacgattt cgccataagt gccgtattcg gtaaacataa aggacggaat
    21361 aatgctcacc ttatcgctaa tggcaacttc gttcacaccc tcgccaatcg ccacaatcac
    21421 accagcccct tcataaccca aagtcgcagg gaatacaggg tcaatcacat acgccccttc
    21481 acggtacatc atttcggcac ggtttaagcc aatggcttgg atttggattt gtacttcgtg
    21541 tgcttttggt gcaggaattg ctacatcaac gatttgcaag acatcaggcg aacctgtttt
    21601 tgtaaattgg atttgtttgc tcattgtttt gaccttttgt gtaaataatg ggcgtatttt
    21661 aatgcaatga atttatttta aacacaacat caattgcaaa acacttttgc taaatttaca
    21721 taaatacatt ttcacaatgt ctgaaaagtt gttttgcaaa ttttaaaaag aaaaccaccg
    21781 cttgccgatc acaagcggtg gtttttgtgg gttattttgc aagaggtgtc agcttggcta
    21841 cagtaagcgg ataaatgcga cgccggctaa gagtaaggta acgccgatga tctgcagcgg
    21901 tagaatgggt ttttgtgtaa tgccgaacag accgaattta tctaccagca ggctgccgca
    21961 aatcaggcca gagaggacga gcatcaccac cgagcctgtg ccgatttggg ggacgagcca
    22021 aacgccagcg aggataaaca ggctgcccag tatgccgccg ctccatatcc aaaacggttt
    22081 gccttcggtt ggctggcaga gtttcggcag gcttttttca taaaacagca ctaccagtaa
    22141 taagccgatg gcaccgacac cgaaggagac tgttgctgcg ccgagtgatg agccgggctg
    22201 tctgccgaat tcgccattaa cggcggcttg ggttgctaat gcggttccgc ctaatatgcc
    22261 gactgcgcgc cacagccata aatttgcgtt ttctttgcgt tttagaccgg acagatgggg
    22321 cagtaccacg gcaatccata cgccgaataa gactaaacca atgccaaaca ggcggctggc
    22381 cgtgaaggct atgtaaggcg agttaagcca agcgaaagtg tcgatcagca cgcccatcag
    22441 cacttggcct aaaatcggca tgaccgcagt ttgtacgccg ccgagcttgg ggaagatcag
    22501 tacgtttgcc gttagcccca ccatgccgag taatccgccc gtccatgccc ataaggggac
    22561 gttgcctaaa ttgtcgaggc taaatgctgg tgtttgttgc atgatgagcg agagtccgag
    22621 tagaaacagg aggcctacgc tgaaagagac aaacgaggtt aaaaatgccg cacccacgtg
    22681 catacgcaaa cgggcgttga tggctgattg aaatgcaatc agcacgccgg ctaaaatacc
    22741 gataataaat gtcatgacta actgggttgt gctgccgcct caaaatgagt ggtggttgaa
    22801 aagaaatgcc gtctgaatgc ggattcagac ggccttaagg cttggtatcg gcgtttacct
    22861 gatgttgaat agccgttttg cctagccggt ttattcgatg aatttcacca cggtttcgaa
    22921 atctttacgt gctttttggc ggatttcttt atcacggtaa ccgaaggtac acatcaccga
    22981 tacgccccat tcgcttgggt caaataatcc ttccgctgcc aaaatatcgt tgacttcttg
    23041 gtaagcgaaa ccttctatcg gacaagaatc tacgccaatt aaagctgcac cggtcatcat
    23101 attgcccaat gcgatgtagg tttgtttgct tgcccagtca aacaggctgc gttcgctgtc
    23161 taaaatgccg atatctttct cttggaattt tttatacacg gccatggctt tttcgcgcgc
    23221 ttcgccttgt aaacctcgtc tatccataat ttcagaaaaa tattggctgt cgtaacgggc
    23281 atttttcttc gctaaaatca ccacgatatg gcttgaggtt tccatagttg gaatgcccca
    23341 gcagtaaggt ttgatttttt gccgtaattc agggttttgc aacactaaaa actgccaagg
    23401 ctctgagcct accgaacttg gacttaaacg accgagttcc agaatatagt tgaaatcttc
    23461 agccggaatt tttctgctgc cgtcgtaaga acgggtagat gcacggtaat gaaaggcatt
    23521 gattacgtct tctttgttga aaaattgcat ggaacactcc taaaaatgca aaacagtctt
    23581 ttcagacggt ctgaaaagtc tagatgaaat acggataagt ataaaagatt taagttattt
    23641 ttaataattg ataattttga tatttaggta taagttgacg tgatgagaaa aaacagaccc
    23701 tctgaatgtt caggcggtct ttaacgttgg ttgtttacat aaataatccg cgttcatatt
    23761 gcttcggtac aaactgcatt tcactgctgt ctgctgcatt ttggtcatat tgcgcattca
    23821 atacccagta cggatcgcgc aataaggctc ttcccacagc cactaaatcc gcatcgccca
    23881 tacctaagat atgatcgcct accgccggat tatccaacat acctactgca attaccggtt
    23941 tgcccgtagc gagcttcact tgacgggcaa agtctacttg gtaacctgcg taaaattcgg
    24001 ggtgattacc tgcatgcaat acaccatcac caccaccgct gatgtctaag acatctgcgc
    24061 ctgcatcggc aaaacgtttc gctacctgca aaccataatc caagccaaag ccgttttcac
    24121 cgtattcttg tgcagaaatg cggacaatta acggcatttc agacggcatc acttcacgtg
    24181 ctgcctgaat cacttgctcg ccaaacaaca ttttgtcttg gccgtactca tcactgcgct
    24241 gattggattt cggcgaatag aattggtgaa ttagataacc gtgtgcgccg tggatttcaa
    24301 ttgtatcaaa gcctgcctca acggcacgtt tgaccgaatc cttaaaggct tgaaccaatg
    24361 ctttgacttc gtctgtcgtc agctcgcgag gtgtaatcag attttgtccg gcataatcca
    24421 actcaccgta gtgaattgca cttggagcca ctgcatccgg ctcgtcttgg gctttgcgtc
    24481 cggcatgggc gatttgcaca ccgatttttg ccccttgagc atgaacgctg tctaccagtt
    24541 ttttgaatgc ctgttgttgt tcatcgttcc ataagcctaa gcagtttggc gtaattctgc
    24601 cgtttggcgc cacattggtc atctccacaa taatcaagcc gacaccacct actgcacggc
    24661 tggtgtaatg cacgaaatgc caatcgttcg gtacgccgtt ggttgccgaa tattgacaca
    24721 ttggtggcat aaccacacgg tttttcaatg ctaagttttt aatgttaaac ggggtgaata
    24781 attttctgaa cttcgccatg ttgccttcct taatgaaata gattaggtgg aatgataatt
    24841 cagtgctatt atctattcaa attgattatt gtgatagtta ggtataaaaa tgagtgatat
    24901 aagaacactg gatttcaatt tgctcaaagc ttttgtggtg ctgctggacg aatgcaacgt
    24961 tagccgggca gcacaacgat tatccatcac ccaaccggca atgagcggta ttttaaaccg
    25021 cttgcgcgag agttttaacg atccgttatt tgtgcgagtg caacacggta tgcagccaac
    25081 cgaccgcgcg ttgcaactgg ggcaaaccgc acgcaacatt ctgcaagata tcagctccat
    25141 gctgcaacca cccatattgg aaccggaaca tctgaatatg accttgcgta ttgccgcaat
    25201 ggattatgta caacaaatta ttgccttgcc gctgattctg cgtttaagac ggcttgctcc
    25261 gaatgtaaaa gtcgccttac tgccggtgca ggggcaaaat atcaaaactt tgttcgagca
    25321 aaaaaaaatc gatcttgcct tggtcagccg ccaacattta agcacggata cgctgaaaac
    25381 ggtattatat gaagaacgct acgtatgcgc gatgagcaaa acacacccga tggcgcaaac
    25441 accgctatcg ctcgatcaat tctgcaatct tcccttcgca atgctttctt acaacggcgg
    25501 agaattcagc ggagctacgg atctagcgtt acaaaagata ggactacagc gcaaagtaat
    25561 ggtctcggta aaccatattt ccctgctgcc ccagctgcta caaggttccg acttagtcgc
    25621 agtattgccc gaacgcttag caaaaacctt acccaatgtt tatttacaac ctccgcctat
    25681 agaggtagaa ggcttcacca tgatgatggc ttggcacgaa agaaccgaac aagatacggc
    25741 gcatcgatgg ttaagagagg tgctgcaggg tgtggtaaaa cctaaacagg ctctttaatt
    25801 attctttctt ttgctatgaa aaaagccgtt tatattttta gataaacggc ttttttgcat
    25861 tctaatatta gtatttttct acaaactgag cataggcatt aatgaagttt tgcattgagc
    25921 tttttaatgt ctcagaggca atttcaccct gttcatcaaa tacgcctgta aataagtttc
    25981 ctacgtttaa tgcgaaaggt aaggttggca tatttacaaa tgagcctgaa gtaatagtac
    26041 gtacttgatc agctgcacgt acaccgcctg ccgggcttgc gccaacggtt acgatacctg
    26101 ccggtttgcc aatccatttg ctttgcccca ttgggcgaga aaccacgtca atcgcatttt
    26161 taagcattgc agaaaagctt gcattatgct caggtgtgac taataacacg ccatctgcca
    26221 cagcaaccgc ttcacgcact ctggtgtatt gtgccgggct gttttcatct aaatcacggt
    26281 cgtaaagtgg taagtctgca atatccacaa tgtttaattg aatgcttgca ggtgccattt
    26341 tttgtaagtg acgaacagtc aagtgactaa atgaagtttt gctgccgcta ccaattaata
    26401 ctgcaatttg ttttgccatt ttataattcc ttagtacata gagaatatag gcattttatc
    26461 cctttcaatc tgtttcataa agcgc
//
LOCUS       DANLRK010000013        24199 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.013, whole genome shotgun sequence.
ACCESSION   DANLRK010000013 DANLRK010000000
VERSION     DANLRK010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 24199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24199
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.013"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(36..1148)
                     /locus_tag="PXA33_000393"
     CDS             complement(36..1148)
                     /locus_tag="PXA33_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5119763.1"
                     /translation="MKNNKTDTLLFLEQFARYLTAKSWQIAFELEYAVIWQKDLGHRI
                     LEVTLPKPIAEDREEVLQKALEKLAKIEEKNVETLKLNISNQYTDKLSVRVIGESVKD
                     GTIPLNEGVKLFEKTKHLINALALSAKKKKAAFGHNSGGKDVAEFMSQVRLGQTQIGS
                     YIVNLSYPVENIEIKEQNEIIQAISFSRSVSHNLVNSLNKLKEKITHYDHNPTIFAEL
                     IPEGVSHNLCEAIIGLSGSDQQRKIEIKLQVGEISDESMPSETFNIKFSQKEIKVIQV
                     ASRYYQGEYTLPHYEIIGKVVGLHSSNLADGGYIQVPCKIEDKTVDIRVDLPPEQYKL
                     AAEAHKTEQQIRCKGENLYINKKKGRLQKLTSINIL"
     gene            complement(1145..1648)
                     /locus_tag="PXA33_000394"
     CDS             complement(1145..1648)
                     /locus_tag="PXA33_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4365 domain-containing protein"
                     /protein_id="HDL5119764.1"
                     /translation="MLSPTQQMEQFNIAYVLAITANAGFNHAVPVVDNHSVDLAISAE
                     FPTEEGKRSDPEIKLQLKSEGDLHIKNGLVSYKLKKKNYDDLRKNCANPRYLIVCDLP
                     KKTTQWLSHKKKFMTLKRHCYWVSLKGFPATTNRSSVTLTFPENQRFTTDVLIKMIEY
                     ARVGETL"
     gene            complement(1653..2384)
                     /locus_tag="PXA33_000395"
     CDS             complement(1653..2384)
                     /locus_tag="PXA33_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL5119765.1"
                     /translation="MRPLKEIRYDNLLRLIDEAKSTSDLANRTGIAVSYLLQIKNKNA
                     IQNGKPKGIGDKIAAKLEDGMNKPRGWLDQIHSEQSNLINSRIGNEEQKLQEQDLNDF
                     IIIDVLDVSASAGFGSSSELVEVVNQMRYVPEQFYSLFRNMAPRYIRIINLSGDSMYP
                     TFSSGDMLFVDISVNEFTGDGVYVFTYKGHLYVKRLQNTGDQILVISDNKLYEKWSIT
                     EENQDQLFIHARVKVHQSQQLNFIG"
     gene            2511..2735
                     /locus_tag="PXA33_000396"
     CDS             2511..2735
                     /locus_tag="PXA33_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL5119766.1"
                     /translation="MQLQEYIEKSKWGEISRLAKAIDVAIPIVSFWVNGKRQVPAKRC
                     PEIEKFTEGKVTCEELRPDVDWAVLRNSGK"
     gene            2784..3545
                     /locus_tag="PXA33_000397"
     CDS             2784..3545
                     /locus_tag="PXA33_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HDL5119767.1"
                     /translation="MKSQSLLPITAQNASGAEVKISSIEIAELCEKQHKNVIVDIRKM
                     LAELKLNSADFSAQYQTKDGRMQPCYFLPKRETMILVSGYRIDLRAKIIDRLDELEKQ
                     HQPSIPKNYPEALRMAAELAEQNQVLLLEKQQTTAENHALKSYFEPGLTPAQFVKGLN
                     GVNSNKINDYLRSRGWLYKDKHCWRVFSHVRDKYLTESFKRIEVDPVERIEMATYKPI
                     LLEKGAVKIFEAYLQGKLPMKADWNGKYYHSKVAV"
     gene            3542..4333
                     /locus_tag="PXA33_000398"
     CDS             3542..4333
                     /locus_tag="PXA33_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL5119768.1"
                     /translation="MSMLLMVKAMNCTVGNSARKLVLLKLADNANDDGVCFPSYQFIA
                     DKCEMSKRSAISHIDELIKMGYVSKKSRKNKDGSSSNLYQLHLDAPSEKSALGGENFA
                     PGGSENSAPITSHSINQSINHNTSEQNSDGVDKKSKYRFSDNDMAVAKWIFKRVKQLA
                     PQTKESYLPNWANEVRLMVERDKHTHQEICSLFKFATEHHFWGRNILSVVSLRAKWDR
                     LNLERQSSLRPNNIHSATGNGDIQDTTGFSKGLVVDVGGGTKWEF"
     gene            4321..4956
                     /locus_tag="PXA33_000399"
     CDS             4321..4956
                     /locus_tag="PXA33_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HDL5119769.1"
                     /translation="MGILATTTQNQVLPVEIVEQAEVETTKIFNRLKATHLGWRANIK
                     SQAEYDNARREWVRLLIENRLTPKEVERGLKIADTDKNPYLPSFGQFLEWCKSIDYAE
                     LGLPDMDQFLKRLNHFSAFGFTEIDNFKFRSNAEYWLLTDLYERNRKHSWKDETLRNQ
                     AEKALVEMAKRIMSGEQIPPPKPVIEEKKKVPIDPRIQRILDEKKQAGAMQ"
     gene            4953..5525
                     /locus_tag="PXA33_000400"
     CDS             4953..5525
                     /locus_tag="PXA33_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine methyltransferase"
                     /protein_id="HDL5119770.1"
                     /translation="MTDFDKDTYQTPNYVRNWLNHRYAWFHIDGCSNGKNALYSHWIG
                     KAAEGLDEDTLSGQIAEDFLAENLLDVILECVADWGELLRIFVNPPYSNPLPFVQRAA
                     ELKKAGHIVVMLLPADKSTRWYQVIQENASEVIDIIGGRINFINPLTDEEVKGNSKGS
                     MVAVFDPFVEGIVTRQMPLDKIKELGGYEK"
     gene            5595..6623
                     /locus_tag="PXA33_000401"
     CDS             5595..6623
                     /locus_tag="PXA33_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HDL5119771.1"
                     /translation="MAEGLLLTPYFQTEVGIVFYKVPKDVSLAAFGERTLLQPAPTDL
                     ATVKSGKVANFSANSTACKELAEFAKSAKVRSTLNQRDKYSNDLPYTRFVKSIQTCQC
                     RDGKYCHHEKVFVENEKGAVQLCWYHDKMRMDGEIPNEKLEALADENWEKFIAQQIRL
                     TLRKTQSSAIEFADLVLFASIKGLLDELDSNALQQFLGYPPLNNSGVSKESNIGFDDV
                     PNARANLKKWAETLKLKVEPEPLAAFMAKPKLQRFEWRKWLQFVKAQPCMCCGQQADD
                     PHHIIGYGGAMGSKQHDLFTLPLCRIHHDELHRNVGKFEQDYGSQLELLIKFLDRAIG
                     LGALEIDG"
     gene            6616..6975
                     /locus_tag="PXA33_000402"
     CDS             6616..6975
                     /locus_tag="PXA33_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HDL5119772.1"
                     /translation="MVELVLPYPPSVNDYWRWVSKTRRVVCKRGKDYQWATFLAAQNK
                     PKFGGQVEIKCDVYFPDNRDRDLDNLGKCILDSLVYSKVIVDDSRHYVKKLTFEDKGN
                     QKGGAVIVRIKERLDVN"
     gene            6965..7324
                     /locus_tag="PXA33_000403"
     CDS             6965..7324
                     /locus_tag="PXA33_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5119773.1"
                     /translation="MLIDIKGILRFWGYSANGRLGTEFPCVAAGMKPAVSASNHRILR
                     LTDESIFEIDRCIKQLKEQEPQQYEVLIGRYAARVSDTQIEKVLGISHSTFKRELAQA
                     EMYVLGVVVGLKLALVV"
     gene            7404..8210
                     /locus_tag="PXA33_000404"
     CDS             7404..8210
                     /locus_tag="PXA33_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005620883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HDL5119774.1"
                     /translation="MKKLVLVTALSLVLSACGSGGGGGSSASNPNKEATPQSPSKSET
                     KSDTSSQNQVKGALSKVSADLQEQVKALKQINVDGRIIELAQEPIGFIEKDLDNGKKG
                     KAYNLAYSSIGYVLPKDVKTDEYGRVIDERASANDVGIFGLATKFQDLPKSGTAHYSG
                     VSFGANSEGKLTLTADFGDKKVNGSITERKLLSNNQALSSINLLSAPISQTKFAGEEI
                     HFAGIAQANVEGNIVQAPYGGKFMGPKAEEVVGFVADDHNDPYEVFAGQK"
     gene            8374..8721
                     /locus_tag="PXA33_000405"
     CDS             8374..8721
                     /locus_tag="PXA33_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HDL5119775.1"
                     /translation="MPEKNPDIYTQAWNYFAMFVSNHSSFICAVIIAFFTSITKSFLY
                     GKTDTPKRVFAEAILCAVIAGSLRPILIYFNADVSLITPIGAGIGLLGTSAIRQMILK
                     FLNKKAGVDDNVN"
     gene            8711..9307
                     /locus_tag="PXA33_000406"
     CDS             8711..9307
                     /locus_tag="PXA33_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 19 protein"
                     /protein_id="HDL5119776.1"
                     /translation="MLISERKFKQVFPSAIDGIYAEIAKHIEKAGCKTKEQQAMFLAQ
                     CGVECAGYKTFEENLNYSATALLATFPKYFTQHNVVLYARDKRAIANRVYANRMGNGN
                     EQSGDGWKYRGRGLIQITGKANYIEFAKWEGLPSLVTSPDQITDNLALTVQVAVWYWQ
                     SRKLASYGSDILKVTKLINGGTHGLSQRTNYYQRLMVA"
     gene            9308..9658
                     /locus_tag="PXA33_000407"
     CDS             9308..9658
                     /locus_tag="PXA33_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2570 domain-containing protein"
                     /protein_id="HDL5119777.1"
                     /translation="MSKYIYLVATILILGLCGWIWQQSKQINTLRAENQTQAQTIEQQ
                     QKANQRLADKLEQERQAVEKIRKSANEMRNKVEIVQNEIKSILAQDSCAKADLPNGVA
                     DSIKRLHRQNNHKH"
     gene            9663..9836
                     /locus_tag="PXA33_000408"
     CDS             9663..9836
                     /locus_tag="PXA33_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5119778.1"
                     /translation="MYPPAAYLVPCERTEFSGNTYGDTVEYLIKVMGERDLCANQIDR
                     IREWQAQTKQGFK"
     gene            9972..10370
                     /locus_tag="PXA33_000409"
     CDS             9972..10370
                     /locus_tag="PXA33_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5119779.1"
                     /translation="MIVKLKLGAVVVLSQVKQATIAYAGSEAYQTAIEQAKKGLLDED
                     KGKPFETVLHFPAVYADEDGQEEICPEQIATTVRSDNVSGEPVAVVLIPDDNVVGELQ
                     SIFIYKTDYAEIFTDNGVKLSAVRTSPAPR"
     gene            10518..10994
                     /locus_tag="PXA33_000410"
     CDS             10518..10994
                     /locus_tag="PXA33_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HDL5119780.1"
                     /translation="MDNLFDLKLNINQIAELTGLHRQTVSQRVAGLNPALGSNSKLKL
                     YALRDLILTGLAEKMSADVDSLSPADRRAWFQSENERLKFEKEIGELIPASEVALEMS
                     ALAKTVVQALETLPDILERDCGLQPKDLIRVQQVTDDVRDQMALHIQEVTTDTENE"
     gene            10994..13105
                     /locus_tag="PXA33_000411"
     CDS             10994..13105
                     /locus_tag="PXA33_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010786315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HDL5119781.1"
                     /translation="MFASAKEIRRDIANAIKAPRRMRVSEAVAEYMRVPLGGGNSVKW
                     DKHTAAYMLEPMDCLNSREYDAVIFVGPARTGKTIGLIDGWITYSIICDPSDFLLVQL
                     TQEKASEHSRKRLDRTFRCSPEIAKRLSPRKNDNNVHDKYFRAGNLLKIGWPSINVLS
                     SSDYKYVALTDYDRWPEDVDGEGDGFSLASKRTTTFMSAGMTLVESSPGKDIVDLKHI
                     PKSTHEAPPTTGILSLYNRGDRRRFYWQCPDCSEYFEPSMANMVGYREDSDFVKASEN
                     ARLQCPHCQTLIAPELKRELNIKGVWLKEGQKIDASGQISGESRKSRIASFWLEGPAA
                     AYQTWAQLTYKLLNAEHEYEMTGSEETLKAVTNTDWGLPYLPRSALEQRRSDELMERR
                     EEVEEKTVPAQCRFIVAAVDVQGGKNRRFVVQMVGYGENGERWLIDRYNISHTLPDSD
                     GVIEKIDPRIPDDWYILISDVLEKRYPLAHNPNHFMPILAMAVDSGGEDGVTDNAYQF
                     WRKCRRDGYAKRVYLVKGDSTKRQKLITKTYPDNTARSDRHSSARGDVPLYLLQTDYL
                     KDRINNALFRDTVGANYIHFPDWLGEWFFNELTYEERGADGKWRKPGKGNNEAFDLFC
                     YAHAIAILRGYERIKWGDENDVPSWAKLPDINPNIIRETPRTAEQAVEIEEKSEKPQP
                     KPTKAKSSWLSGGGRKTGGWL"
     gene            13102..13326
                     /locus_tag="PXA33_000412"
     CDS             13102..13326
                     /locus_tag="PXA33_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5119782.1"
                     /translation="MSLYTIDELKQKIRTLDEKIETAQSQVSFNGRSVSYQVAELTKQ
                     RDRYQTMLDEQLAQSGQRTKQHRIKYARFI"
     gene            13326..14825
                     /locus_tag="PXA33_000413"
     CDS             13326..14825
                     /locus_tag="PXA33_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDL5119783.1"
                     /translation="MNFLEKTIATLSPKWAANRSRNRYVLNAYEAAQPSRTHKASRES
                     KGANSTVRQSAVSLREQARALDQNHDIVIGILDKMEERVIGSKGIHIEPQPLTLAGEV
                     HEELAGQIRKLWAEWSVKPDVTGLYTRPLLERMLLRTWLRDGEVFVQLVKGKVAGLEH
                     GSQVAFSLEALEPDFVPMQSDEAKNGLVQGVFLNAWRKPTAYQVYLDNPQESNGMYGK
                     VKTVPADNMLHLAFRKRLHQIRGVSMLHGVIVRLADLKEYEESERVAARIAAAMTMYI
                     KKGDAALYDDDSNSDGERLFDIAPGAVIDDLKPGEDIGLINSNRPNVNLETFRNGQLR
                     ATAAGTRSSYSSIARDYNGTYSAQRQELVESFEGYAVLQDAFVAAISRPIYREWLKMA
                     IASQAIKLPPDIDQNSLFNAVYSGPVMPWIDPIKEANAWKERIKGGLATEGQAIRASG
                     NNPAEVKRQRIVEIKENQAEGLKFDTDLTNYGGQDKSFSNQKDEINEDE"
     gene            14837..16798
                     /locus_tag="PXA33_000414"
     CDS             14837..16798
                     /locus_tag="PXA33_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HDL5119784.1"
                     /translation="MKTWFNIQAKANDTAEISIFDEIGFWGVTAQQFAKDLKALGNNL
                     KQINLHIHSPGGDVFDGIAIYNLLKNHPANKTVYIDGLAASMASVIAMAGDEIIMPEN
                     AMMMIHKPWGIQGGDADDMRKYADLLDKVESTLIMAYVAKTGKYETDLAEMLKEETWL
                     TGKECVEQGFADKLAEPLVAMACIQSKKLEDYTKMPDKIKNMLFAPQGNAGANPKHEQ
                     PQTPPQAGENPQNVAKPDAMVALAQRNATIKATFAAFGTQFDGLLAECLADVSMTAEQ
                     AKDKLLAKLGENTTPSVPQNHIHAGNGNIVGDSVKQSLMARAGQDTDKTNARDNAYNA
                     MTLRELARASLVDRGVGIAGYTPMQMVGLAFTHSSSDFGQILIDVAHKSLLKGWETAT
                     ENYEQFTTRGTLTDFRPAKRVGLGEFGYLPEVGEGEEYTYGTIGDEGASVALATYGQL
                     FSITRQAIINDDMHLLTKIPEKMGAAAKATITKLVFALITGNATAQDGKKLFDATHKN
                     SLTGAALNVENIDKAIQLMNGFVNSRGEPLSIEPEFMLLPTSLHTKAKQILGSSSVEG
                     ADINSGIINPIRDIVSPIKSPRLQIADPKSWYLLNKEAIEVSYLDGVDSPYIEQQHGF
                     TVDGVSTKVRIDAGVNVIDYRGVVKVTNA"
     gene            16872..17195
                     /locus_tag="PXA33_000415"
     CDS             16872..17195
                     /locus_tag="PXA33_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2190 family protein"
                     /protein_id="HDL5119785.1"
                     /translation="MAKNYIQDGNTVRLTATKAITSGDVIVAEDLIAIAVSDAAKNDA
                     VVGLTTGVFSVKAKQADDIKQGAVLYWSETEGATLTAGSNKRLGIAWKDSGTSSNQVD
                     VKINV"
     gene            17188..17490
                     /locus_tag="PXA33_000416"
     CDS             17188..17490
                     /locus_tag="PXA33_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL5119786.1"
                     /translation="MSSPFEQAIAAADQTIQQTMMSEWLIGGKPYPATFDEAPAIFNG
                     LHSTDDRAIHGTERTLTLFRASGYKPRLDDRVSGNGKKYLVKSYHFVDQLIVLQLE"
     gene            17494..18018
                     /locus_tag="PXA33_000417"
     CDS             17494..18018
                     /locus_tag="PXA33_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL5119787.1"
                     /translation="MGSQVTGLKELQASLKKIARQTVPKAAAQAIRTVGRQAMNKAVK
                     SVAAEIGVNQKTIKGRAKMTAKPTPSRLQATIKVNRSHMPMIRVLERKSNRLSPTKGS
                     IRVGKHTVQRGFRQRLANGRTHIMYRQGRKRYGIDVAKVPLSQPLTQAFDRELKNYPE
                     QVQAELAKQIAGKL"
     gene            18015..18410
                     /locus_tag="PXA33_000418"
     CDS             18015..18410
                     /locus_tag="PXA33_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL5119788.1"
                     /translation="MKIHTKIRKEVLELLQTKLPEIDNFYNGLPTFIDIEEEQLAVSV
                     YLDDISLQEITVCNNQWDAKLNITIHLKSTESAEDELDEWAEKIREIIDDYPEFEHLE
                     SVALSQYQYEQDQNQRTWHMATLIFDVEY"
     gene            18438..19079
                     /locus_tag="PXA33_000419"
     CDS             18438..19079
                     /locus_tag="PXA33_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5119789.1"
                     /translation="MAKTTKVQGTKFRISVGREAQKAITAINLNTATLTIASSGYKKG
                     DAIELTGCGQLDGIYPVLSVTTDQVKLCEEVNWTGKDLPANYTKAKAALVQYSEQFCA
                     VKNIEKSDDTLSTEDVTTVCSEGTETEPGEIEFGSIKLSFFHKPTTEMQTRLRKLFYD
                     KSTFAYKLELPDNHGTTYGEGFIEAGNGFSGEVKGKYEGSVSIKPSKRDYLLV"
     gene            19163..19564
                     /locus_tag="PXA33_000420"
     CDS             19163..19564
                     /locus_tag="PXA33_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5119790.1"
                     /translation="MTLREKLLANKPKLQPIEINGETYYLREATVGDMNKQIFETRSW
                     LIQQAEQENVELPAEDDETFDEALNRFGEKYRLAQSVAYRLCDENGVLLFNPLDINDL
                     NAIAELDSKVIIDFNQAVSAPKDSASEESSK"
     gene            19609..19839
                     /locus_tag="PXA33_000421"
     CDS             19609..19839
                     /locus_tag="PXA33_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HDL5119791.1"
                     /translation="MPERHFAEYRLFYQEQPFGLWRDDYRTAQLAHLTAMFNRDPKGK
                     APKLAEFMPFFNRTDEVEEIEDDGVADYLAKR"
     gene            19902..20129
                     /locus_tag="PXA33_000422"
     CDS             19902..20129
                     /locus_tag="PXA33_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5119792.1"
                     /translation="MFEALDRFMINTIKIAIIVFLVACIFISMPNWWIASILVLFPTA
                     YLLDKYWWRNTEMYRRKMDKLVEEMKNNRQI"
     gene            20183..23749
                     /locus_tag="PXA33_000423"
     CDS             20183..23749
                     /locus_tag="PXA33_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HDL5119793.1"
                     /translation="MSSSNRLTITLDVENSKLLAGFAQSDTVANKFARSFIVNMDQAQ
                     QKARQFADRSTKYLKNIEQAANNLNSSVSKERFWQIANRLQFPTSKVISYANSYTELG
                     NKLKLVTESESQHARAMAAVYDISLKTAQSTQATSSVYQTFAQNAKQLGISQNDVAKL
                     TETVAKSVAISGASSATASNALVQFSQSLLMGKLKAQEFNSLMTQTPSVIQAIAKGLG
                     ITTAELKAMVDNGEMSAEKMIEGLKKAESYVNGQYAKTTTTISGAMQNLSTAAEKWVG
                     EMDSTVGVSQKAVAVLNALNANFDTVAKTVLVAGGAFALFKGYMNVRPFIESRAAIYE
                     NQKAIIAENQARQQAMAIRQQELVGLSQQTALKLEQTLQTYNQNKAELEAIAVKQQKI
                     TKEREEIAVAIQAAATRSERLALSAELQALDQKEITLGQQKIAVERQQIAAKQALKVA
                     YAENAVAQSAMSAGMITATTTTRAMNGVLAAARAELNLLKAAALSNPLMTLAMVATTA
                     GTALWGFFESQKAAREEALRYADSLDSVRVNIEKMTKAQTDAEMVKLSRSIKEQETAL
                     ANLKQRQAELTREIEQGATVYEDAFAGTVRVTMSAEELAKKQEELTLVTADAEKAQKT
                     LNSSLEAQEALKAHIPIANLREEFVKLYPHIDESQIKVDGLNIAIGNFTVKSPEMVIA
                     TNNIANALGGVAGEAMRAAILVANLSSMKLDANGAAIIDPKHLEKIEQIERNNAIGSA
                     KGKDKIALQVKDALIKSGMKEGDAGYERLKAAYEQQFTLQNAPKGKGGKTKAEKEAEK
                     AAKKQQSYQNQVAEMTNRLAGLKANASDIAIFGQVSDYQEVRKLTEDIAINAEKYKGY
                     GEQGVARLKELASQLESAQQQVAISQFAYNGGEKLKAMEFELTLLGKTRQEQELMQYN
                     HELDLEAARLKIGMADENIAKLDQEIVKLKERRAEIQAQAEQARGSFKQGMLDGLNNI
                     EADVSNVAANVANITQNTFDGMADSLTNFVMTGKADFRSFANSILSDLSKMIIKMMLF
                     NAIKQGATMLFGYQGPTTTVNGNAVYGLGTWATGGYTGDGGKYTPAGIVHKGEYVITK
                     EATARLGLDYLNYLNYGKRGFSSGGGVAVPRVPSSSYQPKSAQSNISVQVINNGEPVD
                     AKVSQKQQGDQMQITVELMREIARQEANGMIQNNFRAGGVFA"
     gene            23749..24078
                     /locus_tag="PXA33_000424"
     CDS             23749..24078
                     /locus_tag="PXA33_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL5119794.1"
                     /translation="METLKWCVRTDLAVENTPEIMEVKFGDGYTQRSPKGLNNLLRAY
                     TGTIKVKKGEHLAVEAFFAKHRGVSPFYFKDPYTQQNKKVVCSSWPAKMVGLNHWEFS
                     VTFKEVP"
     gene            24078..>24199
                     /locus_tag="PXA33_000425"
     CDS             24078..>24199
                     /locus_tag="PXA33_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HDL5119795.1"
                     /translation="MPVSISNQMKLDLAKLEQNAMLDLYEVDLRTLKDKSGNAGS"
BASE COUNT         7540 a         4887 c         5475 g         6297 t
ORIGIN      
        1 ttaggcaaaa gaaaaccgcc acgagggcgg tttgtctata agatatttat tgaggttaat
       61 ttttgtagcc gacctttttt cttattgata tataggtttt ctcctttaca tcgaatttgc
      121 tgttcggttt tgtgtgcttc agctgccaat ttgtactgct caggaggaag gtcaacccta
      181 atgtcaaccg ttttatcttc tattttacaa ggtacctgaa tataacctcc atcagctaaa
      241 ttgctggagt gtaaccctac aactttgcca atgatttcat agtgtgggag ggtatattct
      301 ccttggtaat acctagatgc aacctgaatt actttaattt ctttttgaga aaatttaata
      361 ttaaatgttt cagaaggcat tgattcatca ctaatttcac ccacttggag tttaatttcg
      421 atttttcttt gttggtctga accacttaat ccaataatag cttcacaaag attatgactt
      481 actccttcag gtatcaattc agcaaaaata gtcggattgt gatcataatg agtaattttt
      541 tctttcagct tgttcaggct gttaaccaaa ttatgcgata cactccgaga aaatgaaatt
      601 gcctgaataa tttcattctg ttccttaatt tcaatattct caacaggata agataaattg
      661 acaatatagc ttccaatttg agtttgccct aaacgaactt gagacataaa ctctgctaca
      721 tctttgccac ctgagttatg tccaaaagct gcctttttct ttttagctga aagtgccaag
      781 gcattaatta aatgtttggt tttttcaaat aattttacac cttcgtttag cggaattgtc
      841 ccatctttaa cgctttcgcc aatcactcta acagatagct tgtctgtata ttgatttgaa
      901 atattaagct ttaacgtttc tacgtttttt tcttcaattt tggcgagctt ttcaagtgct
      961 ttctgcaaca cttcctctct atcttcagca attggctttg gtaatgtaac ctctaaaatt
     1021 cgatgtccaa gatctttctg ccaaataacc gcatactcta attcaaacgc aatttgccag
     1081 cttttggcag ttaaatagcg agcaaactgt tcgagaaaca gtaacgtgtc cgttttattg
     1141 tttttcataa agtttctcct actctagcgt attcaatcat tttaatgagt acatcagtgg
     1201 taaatcgttg attttctgga aatgtgaggg taacactact tctatttgtt gttgccggaa
     1261 agccttttaa tgatacccaa taacaatgtc tttttaaagt cataaatttt ttcttatgtg
     1321 agagccattg agtcgttttt ttaggcaagt cacatacaat taagtagcga gggttcgcac
     1381 aatttttcct taaatcatca tagtttttct ttttgagttt atagcttacc agaccatttt
     1441 taatatgaag atctccttct gatttaagtt gtagctttat ttccggatca cttcttttcc
     1501 cttcttcagt cggaaattct gcagaaattg caagatcaac actatgattg tctactactg
     1561 gtaccgcatg attaaagcct gcattagcag taatagctaa cacataagca atattaaatt
     1621 gttccatttg ttgtgtaggt gaaagcatat ttctatccaa taaaattcaa ctgttggctt
     1681 tggtgaactt ttactctagc atggataaat agctgatctt gattttcttc agtgatactc
     1741 cacttttcat agagtttgtt atctgaaatc actaatattt gatcgcctgt attttgtaat
     1801 ctttttacat acaaatgtcc tttgtatgta aatacataaa caccatcgcc ggtaaactca
     1861 tttacgctaa tatcaacaaa aagcatatct cccgaggaaa atgtcgggta catagaatca
     1921 ccacttaaat tgataattct gatgtatcga ggtgccatat tccgaaagag ggaataaaat
     1981 tgctcaggca cataacgcat ttgatttact acttcaacta attcactgct tgagccgaag
     2041 cctgcactag cactgacatc taatacatca attatgataa agtcatttaa atcttgctcc
     2101 tgcaattttt gttcttcatt gcctatcctt gaattgataa ggtttgattg ctctgaatgt
     2161 atttgatcta accagcctct aggtttattc atcccatctt ctaatttggc ggcaatcttg
     2221 tcaccaatgc cttttggctt accgttctga atagcatttt tatttttgat ttgtagtaga
     2281 tagcttactg cgatacctgt tcgattagct aaatcagatg tgcttttagc ctcatctatc
     2341 aagcgtaata aattatcgta tctaatttct tttaggggtc tcatatccca cctccatact
     2401 atttgcttta ttttattatc atttcgataa catttaaata tgatctattt tgttgatttt
     2461 gatttatctt taagataata ttcacttatc ttttaggtaa ggattatgaa atgcaattac
     2521 aagaatacat agaaaaatct aagtgggggg aaatctctcg attagctaaa gccatcgatg
     2581 tagcaattcc aatagtttca ttttgggtaa acggcaaacg ccaagtaccg gcaaagcgtt
     2641 gcccggaaat tgaaaaattt acagaaggga aagtaacttg tgaagagcta cgccctgatg
     2701 ttgattgggc agttttacga aattcaggca aataaaaaac caccgctgga acggtggcta
     2761 attacaacct aaggaggttt attttgaaat cccaatcatt attaccgata accgcacaaa
     2821 atgcaagcgg tgcggaagtc aaaatcagca gtattgaaat tgcagagtta tgcgaaaaac
     2881 aacataagaa cgttattgta gatattcgca agatgttagc tgagctgaaa ttgaactcgg
     2941 ctgatttttc agcccagtat caaaccaaag acggcagaat gcaaccctgc tactttcttc
     3001 caaaacgaga aacaatgatt ttagtttccg gctatcgcat tgatctgaga gccaaaatca
     3061 ttgaccgctt agatgagtta gaaaagcaac atcagccctc tattccgaaa aattaccccg
     3121 aggctctgcg aatggcagcg gagttggcgg aacaaaacca agttttattg ttggaaaaac
     3181 agcagactac cgcagaaaac cacgccttga aatcctactt tgaaccgggt ttgacccctg
     3241 ctcagtttgt aaaagggctg aatggcgtaa atagtaacaa gataaatgat tatttacgta
     3301 gtcgaggctg gttatacaaa gacaagcatt gctggcgggt gtttagccac gttcgggata
     3361 aatatctgac ggagtcattt aagagaattg aagttgatcc tgttgaacgt attgagatgg
     3421 ccacctacaa gccaattttg cttgagaaag gtgctgttaa gatttttgag gcttatttac
     3481 aaggtaagtt accaatgaaa gccgattgga acggcaagta ttatcacagt aaggtggcgg
     3541 tatgagtatg ttattaatgg ttaaggcgat gaattgtacg gtgggaaatt ctgcccgtaa
     3601 attagtgctg ttaaaactcg ctgataacgc aaatgatgat ggtgtttgct ttcctagtta
     3661 tcaatttatt gcagataagt gtgaaatgtc taaacgcagt gctattagcc atattgatga
     3721 attaatcaaa atgggatatg tgagcaaaaa atcacgcaaa aacaaagatg ggtctagttc
     3781 aaatttatat caattacacc ttgatgcccc tagtgaaaaa tctgcactag ggggtgaaaa
     3841 ttttgcacca gggggtagtg aaaattctgc acccataacc agtcactcta ttaaccagtc
     3901 aattaaccat aatacgtccg aacaaaattc ggacggggtg gataaaaaat caaaatatcg
     3961 tttttctgat aacgatatgg cagtggcaaa gtggattttt aaacgagtga aacaactagc
     4021 tcctcagacc aaggagtctt acttgccgaa ttgggccaat gaagtccgct tgatggtaga
     4081 acgtgataaa catactcacc aagaaatttg ctcgttgttt aaatttgcga ccgaacacca
     4141 tttttggggc aggaatatcc taagtgttgt atctcttcga gctaagtggg atcggctaaa
     4201 tctagaaagg caatcatcat tacgaccaaa caatattcac tcagcaacag gcaatggtga
     4261 tattcaagac acaacggggt tttcaaaagg tcttgttgtt gatgtaggag gagggacaaa
     4321 atgggaattt tagctacaac gactcaaaat caagtattac ctgttgagat tgtggaacaa
     4381 gcagaggtgg aaacaaccaa gattttcaac cgcttgaaag caactcactt aggctggcga
     4441 gccaatatca agagtcaagc cgaatatgat aatgctcgcc gtgaatgggt tcgtttgttg
     4501 attgaaaatc gcttaacgcc aaaagaagtt gaacgaggtt tgaaaattgc ggataccgat
     4561 aaaaatccct atttaccgag ctttggacaa ttcctcgaat ggtgtaaatc cattgactac
     4621 gcagaattag gtttgcctga tatggatcaa ttcctcaagc gtttaaatca tttttccgct
     4681 tttgggttta ctgaaatcga taattttaag tttcgttcaa acgctgaata ttggctctta
     4741 actgaccttt atgaacgaaa tcgaaaacat agttggaaag atgaaacatt acgtaaccaa
     4801 gctgaaaaag cgttagtaga gatggcaaaa cggattatga gcggtgaaca aattccacct
     4861 cctaagccag taatcgaaga gaaaaagaaa gtgccaatag atccacgcat tcagcgaatt
     4921 ttggacgaga agaaacaagc aggagcaatg caatgacaga ttttgataaa gacacttacc
     4981 aaacacctaa ttatgtgcgt aattggctaa atcatcgcta tgcgtggttt cacattgacg
     5041 gttgttctaa cggcaaaaac gcattgtata gccactggat tggcaaagcg gcagagggct
     5101 tagacgaaga cacactaagt ggtcagattg ccgaggattt cttggcagag aacttgctcg
     5161 atgtgatctt ggaatgtgtg gcagattggg gcgaattact gcgtattttc gtgaatccgc
     5221 catacagcaa tccactgccg tttgtacaac gggcggcaga gctgaaaaaa gccggccata
     5281 ttgtggtaat gcttttaccg gcagataaat ctacccgttg gtatcaggtt atccaagaaa
     5341 acgctagtga ggtgatcgac attatcggcg gacgtattaa tttcatcaat ccgctcacag
     5401 acgaggaagt aaaaggcaac agcaaaggct cgatggtggc ggtgttcgat ccgtttgtgg
     5461 agggaatagt tacccgtcaa atgccgttgg ataagattaa ggagctgggc ggctatgaaa
     5521 aataacgaag gctgggacgg tttagataag gcgtatcatt gccttagccg caacaaaaag
     5581 aaatgggggc aataatggcg gaaggtttgc tacttacccc ttactttcaa accgaagtgg
     5641 gtattgtgtt ttacaaagtg cctaaagatg tttctcttgc tgctttcggt gagcggactt
     5701 tgttgcaacc tgcaccgacc gatttagcaa cggttaaatc aggcaaggtt gcaaattttt
     5761 cggcaaattc gaccgcttgt aaagagttgg cagagtttgc taaatcggca aaagttcgct
     5821 caacgctaaa tcagcgtgac aaatacagta atgatttgcc ttatacccgg tttgtaaagt
     5881 caattcagac gtgccaatgc cgagatggta aatactgcca ccacgaaaaa gtattcgtgg
     5941 agaatgaaaa aggtgcggta cagctttgtt ggtatcacga caaaatgcga atggacggtg
     6001 aaatcccgaa tgaaaaatta gaagcactag cggatgaaaa ctgggaaaaa tttatcgccc
     6061 aacaaatccg cttaacctta cgcaaaacgc aatcttctgc cattgaattt gcggacttag
     6121 tgttgtttgc gagtatcaaa ggtttgcttg atgaattaga cagcaacgca ttacagcagt
     6181 ttctcggcta tccaccactg aataatagcg gtgtgagcaa agaaagtaat atcggatttg
     6241 atgatgtgcc gaatgctcga gccaacctca aaaagtgggc tgaaacgtta aaactgaaag
     6301 tcgagccgga gccgttggcg gcatttatgg caaaaccgaa attacagcgg ttcgagtggc
     6361 ggaagtggtt gcagtttgta aaagcacagc cttgtatgtg ttgcggacaa caagcagacg
     6421 acccacatca tattatcggc tatggcggag cgatggggag caaacagcac gatttattca
     6481 ctctgcccct atgccgtatt catcacgatg aattgcatcg caatgtgggt aaatttgagc
     6541 aagattacgg ctcgcaatta gagttgttga ttaagttttt agaccgagcg ataggtttgg
     6601 gggcgttaga aattgatggt tgaattggtg ctgccctatc cgccaagtgt aaacgactat
     6661 tggcggtggg taagcaaaac aagacgagtg gtgtgtaaac gaggcaaaga ctatcaatgg
     6721 gcaacatttc tcgcagcaca gaataaacct aaatttggcg gacaggttga aattaagtgc
     6781 gatgtttact tccctgataa ccgagatcgg gatttggata acttaggcaa atgtatttta
     6841 gattcgttgg tatatagcaa agtgattgtt gatgactccc ggcactatgt caaaaagctg
     6901 acatttgagg acaagggcaa tcaaaaaggc ggtgcggtaa ttgtaaggat taaggagaga
     6961 ctagatgtta attgatatta aaggtatttt acggttttgg ggctattccg ccaacggcag
     7021 acttggtacg gaatttccct gtgtggcggc agggatgaaa cctgctgtat cggcaagcaa
     7081 ccaccgcata ttacgtttaa ctgacgaatc catttttgaa attgaccgct gtatcaagca
     7141 acttaaagag caagagcctc agcaatatga ggtgctgatt ggtcggtatg cagcaagggt
     7201 atctgataca caaatcgaaa aagtattagg tatcagtcac tccaccttta aacgagaatt
     7261 ggcacaggcg gaaatgtatg tgctgggtgt ggtagttggg ttaaaattgg cgttggtggt
     7321 ttaaataatg tgatctgtat cacaacatta attcaataaa tgagataaag tatagactct
     7381 ttttacctat ggagatttta ccaatgaaaa agttagtttt agttacagca ttaagtttag
     7441 ttttatctgc ttgcggtagt ggtggaggtg gtggctcatc agcatcaaat cctaataagg
     7501 aagctactcc acagtcccca tctaaaagtg aaactaagtc agatacttca tctcaaaatc
     7561 aagtaaaagg tgctttatct aaagtttcgg cagatttaca agagcaagta aaagcactta
     7621 agcaaattaa tgttgatggt agaatcattg agttagccca agagcctatc ggttttatag
     7681 aaaaagattt agataatggc aaaaaaggaa aagcctataa cttagcttat tctagtattg
     7741 gttatgtgtt acctaaagat gtaaaaacgg atgaatatgg acgagtaatt gatgagcgag
     7801 cttctgctaa tgatgtcggt atatttggac tagcaaccaa attccaagat ttaccaaaat
     7861 ccggaactgc acactactct ggcgtatcat ttggtgcaaa cagtgaaggt aaattaacac
     7921 ttactgctga ttttggtgac aagaaagtaa atggctcaat tactgaaaga aagctattat
     7981 ctaataacca agcattatct tctattaact tattatctgc ccctatcagt caaaccaagt
     8041 ttgctggtga agaaatacat tttgccggca ttgctcaagc caacgtagaa ggtaacattg
     8101 ttcaagctcc ttatggcggt aaatttatgg ggccaaaagc ggaagaagtt gttggtttcg
     8161 ttgccgatga tcataatgac ccatatgaag tatttgctgg tcaaaaataa tttttaatgt
     8221 atgattgaca aggtcgccct aaaagtatag gatttatgca atccttgcga ttagtgcaag
     8281 tgcattacgc aatactattt ttatagccct gattggttca gccagtcggg gcttttttgt
     8341 tgccccgaaa aagtggggtg gagtatgaat acaatgcctg aaaagaaccc tgatatatat
     8401 acgcaggctt ggaattattt tgcaatgttt gtcagcaatc atagcagttt tatttgtgct
     8461 gttatcattg ctttttttac gtctattacc aagtcatttt tatacggcaa aaccgacaca
     8521 ccaaagcggg tctttgcaga agcgattctg tgtgcggtga ttgccggttc gttgcgtccg
     8581 attttaatct attttaatgc tgatgtttcc ttaattaccc cgattggtgc cggtatcgga
     8641 ctactaggga caagtgctat tcgtcaaatg attttgaaat tcctcaataa gaaagcagga
     8701 gttgatgata atgttaatta gcgaaagaaa atttaaacaa gtattcccca gtgcgattga
     8761 cggaatttat gccgaaattg ccaaacacat tgaaaaagcc ggttgcaaga cgaaagagca
     8821 gcaagcaatg tttttggctc aatgtggcgt agagtgtgct ggttataaaa cctttgaaga
     8881 gaatttgaat tattccgcta cggcattatt agccacattt ccgaaatatt ttacccaaca
     8941 caatgttgtg ctttatgccc gagataagcg tgcgattgct aatcgggtgt atgcaaatcg
     9001 aatgggtaac ggcaatgaac aaagtggcga tggttggaaa tatcgtgggc gtgggctgat
     9061 ccagattact ggtaaagcga attatattga gtttgcgaag tgggaggggt tgccaagctt
     9121 ggtgaccagt cctgaccaaa tcacagataa ccttgctctt acggtgcagg tggcggtatg
     9181 gtattggcaa agtcgcaaat tagcaagtta cggtagtgac attctaaaag taaccaagct
     9241 gattaacggc ggcactcacg ggctatcaca acgcacgaac tattatcaac gattaatggt
     9301 ggcataaatg agtaaatata tttatctcgt agccaccatt ttgattttgg gcttgtgtgg
     9361 ttggatttgg cagcagtcca agcagattaa taccttaaga gccgaaaacc aaacgcaagc
     9421 ccaaaccatt gagcagcagc aaaaagccaa tcaacggctt gctgataagc tcgaacaaga
     9481 gcgacaagcg gtcgaaaaaa tccgaaaaag tgcaaatgaa atgcgaaaca aagtggagat
     9541 tgttcaaaat gagattaagt caatccttgc ccaagacagt tgtgctaaag ctgatttgcc
     9601 taatggcgtt gctgatagca ttaagcggtt gcacaggcag aacaatcaca agcactgaat
     9661 atttgtatcc gccggcggct taccttgtgc cgtgcgagcg gactgaattt agcggcaata
     9721 catacggcga tacggttgag tatctgataa aagtgatggg agagcgtgat ttgtgtgcta
     9781 accaaatcga ccgtatcaga gaatggcaag cacaaaccaa acaaggtttt aaatagcttg
     9841 gcggattaag ccgctatgac atatgggagc gtaacagctc ccttttttat tgtaccgcat
     9901 tacttagtta tgcttttggt tatatcaaga ccataactaa gcaatgtgtt ctattaacat
     9961 aaggattatt tatgattgtg aaattgaaat taggtgcggt agttgtgcta tcacaagtta
    10021 aacaagccac catcgcatac gctggatcag aggcatacca aacagctatt gagcaagcca
    10081 agaaaggttt acttgatgaa gacaaaggta aaccatttga aactgttctt cacttccctg
    10141 ctgtgtatgc cgatgaagat ggacaagaag aaatttgccc tgaacagatt gcaactacag
    10201 tacgttcaga taatgtaagt ggtgagcctg ttgcagtggt attaatccca gatgataacg
    10261 tagttggcga gttacagtct attttcattt ataagacaga ttatgcagaa atttttactg
    10321 acaatggtgt gaaattaagt gcagtaagaa caagtccagc tcctcgttaa ttttgtaaaa
    10381 ggtactcccg aggggatacc cctttccacg gggttgcggc agcgtggttt tcggcaattt
    10441 tttgagtttc taggcatcat catcttccta tgatgttgct gttttttagg cggttttgat
    10501 ttttaggatt tgagcaaatg gacaacttat ttgatctaaa acttaacatc aatcagatcg
    10561 ccgaactcac aggattgcac cgccagacgg tatctcagcg agtcgctggg ttaaatccag
    10621 cactcggtag taatagcaaa ttaaaactct atgctctgcg tgatttaatc ctcaccgggt
    10681 tggcggaaaa gatgtcggca gatgtcgata gcctatcacc tgctgatcgt cgtgcgtggt
    10741 tccagtcgga aaacgaaaga ctaaaatttg aaaaagaaat tggcgagctt atcccagcaa
    10801 gcgaagtggc tcttgaaatg tcagctttag caaaaacggt agtacaagcc ttagaaacct
    10861 tgcctgatat tttagagcga gactgcggat tgcagcctaa agatttgata cgagtgcagc
    10921 aagtaactga cgatgtacga gaccaaatgg cattacatat ccaagaagtt accactgaca
    10981 cggagaatga ataatgtttg ctagtgctaa agaaattcga cgtgatatag caaatgccat
    11041 aaaagcacct cggcgaatga gagtgtcaga agcagtagcg gaatatatgc gtgtaccact
    11101 tggcggaggc aactcggtca aatgggataa gcacaccgcc gcctatatgt tagaaccgat
    11161 ggactgcctc aatagccgag aatacgatgc ggtgattttt gtcggcccgg cacgaacggg
    11221 taaaaccatc ggcttgattg acggctggat aacctactca attatctgcg atccgtctga
    11281 tttcctgttg gtgcaactta cccaagagaa agctagcgaa catagccgaa aacggcttga
    11341 ccgcacattc cgctgttcgc ccgaaattgc caagcggcta agcccacgca aaaacgataa
    11401 caatgtccac gacaagtatt tcagagcggg caacttgctc aaaatcggct ggccgtctat
    11461 caatgtgctt tcttcatcgg actacaaata tgttgctctg actgactacg accgctggcc
    11521 ggaagatgtg gacggagagg gcgacggttt ctcgcttgcc tcaaagcgga cgaccacctt
    11581 tatgtcagca ggaatgactt tggtggagag ttcgcccggt aaagatattg ttgatctcaa
    11641 acatatcccg aaaagcaccc acgaggctcc accgactacc ggtattttaa gcctctataa
    11701 tcgaggtgat cgccgtcggt tttactggca atgccccgac tgctccgaat acttcgagcc
    11761 gtcaatggca aatatggtcg gctaccgtga agacagtgat tttgtcaaag ccagcgaaaa
    11821 cgcacggtta caatgccctc actgccaaac cctgattgcc cccgaactca aacgagagct
    11881 aaatatcaag ggcgtatggc tcaaagaggg gcaaaaaatt gatgcaagcg gtcaaattag
    11941 tggcgaaagt cgcaaatccc gtattgcctc cttttggctt gaagggccag cggcagccta
    12001 ccaaacgtgg gcgcagctca cctacaaact gctcaatgcc gaacacgaat atgaaatgac
    12061 tggcagtgag gaaaccctca aagcggtaac aaataccgac tgggggttac cttacttacc
    12121 acgatctgca ttagaacaac gccgttctga tgagttgatg gaacgccgag aagaagtaga
    12181 agagaaaact gtaccagctc aatgtcgctt tattgtggcg gcggtggacg tacagggcgg
    12241 taaaaaccgc cgatttgtgg tgcaaatggt cggttacggt gaaaatggcg aacgctggtt
    12301 gattgaccgc tataacatct cacatacttt gcccgacagt gatggcgtga ttgaaaaaat
    12361 tgaccctcgt attcccgatg attggtacat actgatttcg gacgtactag aaaaacgcta
    12421 cccacttgcc cacaatccaa accactttat gccgattttg gcgatggcgg tggacagtgg
    12481 cggtgaagat ggggtaaccg ataacgccta ccaattttgg cgcaaatgcc gccgagatgg
    12541 ctatgcgaaa cgagtctatt tagtcaaagg cgacagcacc aaacggcaaa agctgattac
    12601 caaaacttac cccgacaata ccgcacgaag tgaccgccac tcctccgcac ggggcgatgt
    12661 gccgttgtac ttgctccaaa ccgactacct caaagatcgg attaacaacg ccctgttccg
    12721 tgatacggtg ggggcgaact acatccactt ccccgactgg ctaggtgaat ggttttttaa
    12781 cgaattgacc tacgaagaac gaggagcgga cggcaaatgg cggaaaccgg gcaaaggcaa
    12841 taacgaagcc ttcgacctgt tctgctatgc ccacgccatt gcaattttac gaggctacga
    12901 acgaatcaaa tggggcgatg aaaacgatgt gccaagctgg gcaaaactgc ccgacatcaa
    12961 ccctaatatc attcgggaaa cgccaagaac ggctgaacaa gcggtcgaaa ttgaagaaaa
    13021 atccgaaaaa ccacaaccta aacccaccaa agcaaaaagc agttggttaa gtggtggcgg
    13081 tagaaaaaca ggaggctggc tatgagcctt tacaccatag acgagcttaa acaaaaaatc
    13141 cgcacgcttg atgaaaaaat cgaaaccgcc caaagccaag tgagctttaa tggtcggtcg
    13201 gtttcatatc aagtggcgga actaaccaaa cagcgtgatc gctatcaaac aatgctggat
    13261 gaacaacttg cccaaagcgg gcaacgcacc aaacagcatc gaattaaata tgcgaggttt
    13321 atttgatgaa cttcctcgaa aaaaccattg ccaccctttc cccgaaatgg gcggcaaatc
    13381 gctctcgaaa tcgctatgtg ctaaatgcct atgaggcggc acagcctagc cgaacccata
    13441 aggcaagccg agagagcaaa ggggcaaact ctactgttcg ccaaagtgcg gtcagcctac
    13501 gggaacaagc ccgagcctta gaccaaaatc acgatattgt gatcggcatt ctcgacaaaa
    13561 tggaagagcg ggtgattggc tcaaaaggca ttcatattga accgcagccg ctgacgttgg
    13621 caggtgaagt ccacgaagaa ttagctgggc aaatccgaaa gctgtgggcg gaatggtcgg
    13681 tgaaacctga tgtgacggga ttatacactc gcccattgct agaacgaatg ctacttcgga
    13741 cttggctacg agacggcgaa gtctttgttc agttggtgaa aggcaaggtg gcagggttag
    13801 aacacggctc acaggttgct ttttcccttg aagccttaga gccggatttt gtgccaatgc
    13861 aatccgatga ggcaaaaaac ggcttggtgc aaggcgtgtt tctcaatgcg tggcggaagc
    13921 cgactgctta ccaagtctat ttagacaatc cgcaagaaag caacggaatg tacggcaaag
    13981 tgaaaaccgt gccggcagac aatatgttgc acctcgcctt tcgcaagcgg ttacatcaaa
    14041 ttcgtggtgt aagtatgttg cacggtgtga ttgtgcgatt ggcggatctg aaagaatacg
    14101 aagagagcga acgagttgcc gctcgcattg ccgccgcaat gacgatgtac attaaaaaag
    14161 gcgatgcggc actgtatgat gacgacagta acagcgatgg cgaacgcctg tttgatattg
    14221 cccccggtgc ggtgattgat gatttaaaac cgggtgaaga catcggctta atcaattcca
    14281 accgcccgaa tgtgaatttg gaaactttcc gcaatggaca actgcgagcc actgccgccg
    14341 gtactcgttc gagctattcc agcatcgccc gagactacaa cggcacttac tctgcacagc
    14401 gacaagagct ggttgaaagt tttgaaggtt atgcggtgct acaagatgcg tttgtggcgg
    14461 caatcagccg cccgatttat cgggaatggc tcaaaatggc aatcgcctca caagcgatca
    14521 aactgccgcc tgatattgac caaaactctc tctttaatgc ggtttattca ggtcctgtaa
    14581 tgccgtggat tgacccaatc aaagaggcga atgcgtggaa agaacgaatt aaaggcggtt
    14641 tagccaccga agggcaagca atccgagcca gtggcaataa cccagccgaa gtcaaacggc
    14701 aacgaattgt cgagatcaaa gaaaatcaag ccgaagggct aaaatttgat accgatctca
    14761 ccaattacgg tgggcaggat aaatctttct ccaatcaaaa agatgaaatt aacgaagatg
    14821 aataacagga gggtgcgtga aaacgtggtt caacattcaa gccaaagcga atgacaccgc
    14881 cgaaatctca atttttgacg aaatcggctt ttggggtgtc accgcccaac aatttgctaa
    14941 agacttaaaa gcccttggca acaatttaaa acaaatcaat ctgcatatcc actcgcccgg
    15001 tggtgatgtg tttgacggca ttgccattta caacctgcta aaaaatcacc ctgctaacaa
    15061 gaccgtctat attgacggtc ttgccgcatc aatggctagc gtaattgcaa tggcaggtga
    15121 tgaaatcatt atgcctgaaa acgctatgat gatgatccac aaaccgtggg gcattcaagg
    15181 cggtgatgcg gatgatatgc gtaaatatgc ggatttactg gataaggttg aaagtacact
    15241 gattatggcg tatgtcgcca aaacaggtaa atacgaaacc gatttggcag aaatgctgaa
    15301 agaagaaact tggctcacag gcaaagagtg cgtggagcaa ggtttcgccg ataagttagc
    15361 cgaacccctt gtggcgatgg cttgtatcca atccaaaaaa ttagaggact acacaaaaat
    15421 gcctgacaaa atcaaaaata tgctattcgc accacaaggc aacgcaggtg caaatccaaa
    15481 acatgaacaa cctcaaacac caccacaagc gggtgaaaat ccgcaaaatg tggcaaaacc
    15541 tgatgcaatg gtggcattag cccaacgcaa tgcaacgatt aaagcgacct ttgccgcttt
    15601 cggcacacaa tttgacggct tgctggcaga atgtttagct gatgtgtcaa tgaccgctga
    15661 gcaagccaaa gacaagctcc ttgccaaact gggcgaaaac accaccccaa gcgtaccgca
    15721 aaaccacatt cacgcaggta acggtaacat cgttggcgac agcgtgaagc agtcgttaat
    15781 ggctcgtgct ggtcaagata ccgacaaaac caacgcaaga gataatgcct acaatgcaat
    15841 gaccttgcgt gagttggctc gtgcctcatt ggttgatcgt ggcgtgggta ttgcgggcta
    15901 caccccaatg cagatggtcg gtcttgcctt tactcattcg agctccgact tcggtcaaat
    15961 cttgattgat gtggctcata aatccctact caaaggctgg gaaaccgcta ccgaaaacta
    16021 cgagcagttt accacccgtg gcactcttac tgacttccga ccggcaaaac gagtcggttt
    16081 aggtgagttt ggctacctgc cggaagtggg cgaaggtgaa gaatacacct acggcacaat
    16141 cggtgatgaa ggtgcatctg ttgcattggc gacttacgga caactgttta gcattacccg
    16201 tcaggcgatt atcaatgacg atatgcatct tttaactaaa atccctgaga aaatgggtgc
    16261 agcagccaaa gcgactatta ccaaactggt gtttgccttg attaccggca atgcgacagc
    16321 ccaagacggt aaaaagctgt ttgatgctac acataaaaac agcttaacgg gtgcggcatt
    16381 gaatgtggaa aacattgaca aggcaatcca gttaatgaat ggctttgtaa acagccgtgg
    16441 tgaaccgctc tctattgagc cagaatttat gctgttacca acctcattgc ataccaaagc
    16501 aaaacagatt cttggttcgt caagcgtgga aggtgcagac atcaacagcg gtatcattaa
    16561 cccgatccgt gatattgtca gcccgattaa atcgcctcgc ttacaaattg cagatccgaa
    16621 atcatggtac ttgctcaata aagaggcgat tgaagtctct tatctggacg gggtggatag
    16681 cccttacatc gagcaacagc acggctttac ggtggacggt gtttcgacca aagtccgtat
    16741 cgatgctggt gtgaatgtga ttgactaccg tggtgtggtg aaagtgacca acgcttaatt
    16801 taaacaattt aaaacctgac cgcactttta acagtgcggt tttttattat ccgaaaaaag
    16861 gaaaaagaga tatggctaaa aactatattc aagacggcaa taccgtgcgt ttaactgcaa
    16921 ccaaagcgat tacttccggc gatgtcattg tggcggagga tttaattgcg attgcggttt
    16981 ctgatgcggc aaaaaatgat gcagtagttg gcttaactac cggtgttttt agcgtaaaag
    17041 ctaaacaagc agatgacatc aaacaaggtg cagtgttgta ctggtctgaa acagagggcg
    17101 caactctcac cgcaggctca aataaacgac ttggcattgc gtggaaagac tctggcacat
    17161 catctaatca agttgatgtg aaaatcaatg tctagcccgt ttgagcaggc aatagctgcc
    17221 gccgatcaaa ctattcagca aactatgatg agtgagtggt tgattggcgg caagccttac
    17281 cctgcaactt ttgatgaagc ccccgctatt tttaacggtt tacactcaac cgatgacaga
    17341 gctattcacg gcacggaacg cacactgacc ctatttcggg cgagtggcta caaaccgaga
    17401 ttggacgacc gagtgagcgg aaatggcaag aaataccttg ttaaatccta tcactttgtc
    17461 gatcagttaa tcgtattgca attggagtaa acaatgggtt ctcaagtgac cggtttaaaa
    17521 gaactgcaag cctccctgaa aaaaatagct aggcaaaccg tacctaaagc agccgctcaa
    17581 gcaatccgta ctgttgggcg gcaagcaatg aataaggcgg ttaaatcggt ggcggcagag
    17641 attggcgtaa atcaaaagac aatcaaaggt agagcaaaaa tgacggcaaa gcccacgccg
    17701 tcaagattgc aagccacgat taaggttaat cgatcacata tgccgatgat tcgggtacta
    17761 gagcgcaagt ccaaccgcct gtcacccacc aaaggcagta ttagagttgg gaaacacact
    17821 gtacaacgtg gttttcgcca acgactcgcc aatgggcgaa cgcacattat gtaccggcaa
    17881 ggtcgtaagc gttacggcat tgatgtagca aaagtgccgt tatcccaacc acttacacaa
    17941 gcatttgatc gagaattgaa aaactatccc gaacaagtgc aagcggaatt agctaaacaa
    18001 attgcgggga aattatgaaa atccacacaa aaatccgaaa agaagtcctt gaattattac
    18061 agactaaatt gccggaaatt gataattttt ataacgggct gccaactttt attgatattg
    18121 aggaggaaca acttgcagta tcggtttatc tcgatgatat cagtctgcaa gaaataactg
    18181 tttgtaataa tcaatgggac gccaagctca atattacaat ccatctgaaa tctaccgaga
    18241 gtgcagaaga tgaattagac gaatgggcgg aaaaaattcg tgaaattatc gatgattatc
    18301 ccgaatttga gcatttagag tcagtcgcgc tctctcagta tcaatatgag caagaccaga
    18361 atcagcgtac gtggcacatg gcgacattaa tctttgatgt tgagtattaa acaaataatc
    18421 tctataggag acttaaaatg gcaaaaacaa caaaagtcca aggcacgaaa tttagaatta
    18481 gcgttggacg tgaggctcaa aaagcaatta ccgcaattaa cttaaatact gctactctaa
    18541 cgattgcatc ctcaggctat aaaaaaggtg atgcaatcga gcttacaggt tgtggtcaat
    18601 tagatggtat ctacccggtg ttatcagtaa ctaccgatca ggtaaaatta tgcgaagaag
    18661 tgaactggac aggtaaagat ttaccagcta attacaccaa agccaaggcg gcactagtgc
    18721 aatactcgga gcaattctgc gcggtaaaaa acattgaaaa atctgatgat actttgagta
    18781 ccgaagatgt aacaacggtt tgttcagagg gcacagaaac cgagccgggt gaaattgaat
    18841 ttggttcgat caaactcagt ttcttccaca agcctactac cgaaatgcaa acccgattgc
    18901 gcaaactctt ttacgacaaa tccacctttg cctacaaact ggaactaccg gataaccatg
    18961 gcacgaccta tggcgaaggc tttattgagg ccggcaacgg tttctcgggc gaagttaagg
    19021 gtaagtatga aggctctgta tctattaagc caagcaaacg cgactactta ttagtttaat
    19081 tgcaaaaaaa ccataaaatt cgaccgtttg tagaaataac aagcggtctt tttatatcag
    19141 aattttacaa aaggaacgaa caatgacact gcgtgagaaa ctccttgcta ataaaccaaa
    19201 attgcaacct atcgaaatca acggcgagac ctactacctg cgtgaagcta ctgttggtga
    19261 tatgaacaag cagatttttg aaacccgaag ctggctcatt caacaagctg aacaagaaaa
    19321 tgttgaatta ccggcagaag atgatgaaac ctttgatgag gctctcaacc gttttggcga
    19381 aaaataccgc cttgctcaat cggttgccta ccgcttatgt gatgaaaacg gtgtattact
    19441 gtttaatcct ctcgacatta acgatctgaa tgccattgcc gaattagaca gtaaagtgat
    19501 tatcgacttt aaccaagccg tgtccgcccc aaaagactca gcgagcgaag aaagttccaa
    19561 ataacccttt cgctcgcact gggtaaaaca cttgaagaaa tcgaacaaat gcccgaacgc
    19621 cactttgccg aatatcggct tttctaccaa gaacagcctt tcggcttatg gcgagacgat
    19681 taccgcaccg cccaacttgc ccacttaacc gcaatgttca accgtgatcc gaaaggcaaa
    19741 gctccaaaat tagcggagtt tatgccgttt tttaatcgga cagatgaggt ggaggaaata
    19801 gaagatgatg gtgtggcaga ttatttggcg aaaaggtaaa gaaaacagct tgaaattcct
    19861 tgctcttgga tatatatttc ctcaaagaga aggagggaag tatgtttgaa gcactagacc
    19921 gttttatgat taatacgatt aaaattgcaa ttattgtatt tttggtcgct tgcatcttta
    19981 ttagtatgcc gaactggtgg atagcttcta tcttagtact tttcccgact gcctatttgc
    20041 ttgataaata ctggtggcga aataccgaaa tgtatcgccg caaaatggat aaattagttg
    20101 aagagatgaa aaataatcgt caaatctaaa ttaacatcaa ttctagcccg ctttatgcgg
    20161 gctttttatt tggggtgaaa aagtgagtag ctcaaacaga ttaactatta ccttagatgt
    20221 agaaaattct aagttactag ctggattcgc acaatctgat acagtagcaa ataagtttgc
    20281 tcgcagcttc attgtgaata tggatcaggc tcagcaaaaa gcacgtcaat ttgctgatag
    20341 atcgacaaaa tatctaaaaa atattgaaca agcagcaaat aatcttaata gctctgtatc
    20401 aaaagaacgt ttttggcaaa ttgcaaatcg gttgcaattc ccgacaagta aagtgattag
    20461 ttatgccaat agctacaccg aactcggtaa taaactcaaa ttagtcaccg aaagtgaatc
    20521 ccaacacgcc cgagcaatgg cggcggtgta tgatatttcg ctcaaaaccg cccaatctac
    20581 acaggcaacc tcttcggttt atcagacctt tgctcaaaat gcaaaacagc tcggtatttc
    20641 tcaaaacgat gtggcaaaac taacggaaac ggtggcgaaa tcagtggcga tctctggtgc
    20701 aagttcggca accgcttcaa acgccctagt gcaattcagc caatcgttgt taatgggtaa
    20761 gctcaaagcc caagaattta actcattgat gacccaaaca ccgtctgtta ttcaggcgat
    20821 tgccaaaggg ttaggaatta ctactgccga acttaaagca atggtggata acggtgagat
    20881 gtcggctgaa aaaatgattg aggggctaaa aaaagcagag agttatgtaa acggacagta
    20941 cgccaaaaca accactacaa ttagtggagc aatgcaaaat ctttcaactg cggctgaaaa
    21001 gtgggttggc gaaatggata gtaccgttgg tgtgtcacag aaagccgttg ccgttctgaa
    21061 tgcgttaaat gctaattttg acacggttgc taaaacggta cttgtcgccg gtggagcctt
    21121 tgctcttttt aaaggctata tgaatgtacg cccatttatc gagtcaagag cggctattta
    21181 tgaaaatcaa aaagcaatta ttgctgaaaa tcaagcccgc caacaggcaa tggcgattcg
    21241 tcagcaagag ttagtaggac tgtctcaaca aaccgcttta aaactggaac aaacattaca
    21301 aacctacaat caaaataaag ccgaattaga ggcaattgca gttaaacagc agaaaattac
    21361 aaaagaacgg gaagaaatcg cagtggcaat tcaagcggct gcaactcgtt cggaaagatt
    21421 agcgttatcc gccgaattac aagccttaga tcaaaaggaa atcacgctcg gacaacagaa
    21481 aatagcggtt gaacgccagc aaattgcagc aaagcaggct ctcaaagtgg cgtatgctga
    21541 aaatgcggtt gctcaaagtg caatgagtgc aggtatgatc acagcaacca caacaaccag
    21601 agcaatgaat ggtgttttag cggctgcaag agctgaattg aatttactga aagcagcagc
    21661 actgtctaac ccattaatga cactcgcaat ggtggctaca actgccggta ctgccctttg
    21721 gggatttttt gaaagtcaaa aagcagctcg tgaagaagcc ctccgctacg ctgacagctt
    21781 agactctgtt cgagtcaata tcgaaaaaat gactaaggct caaactgatg cagaaatggt
    21841 taaattgtct cgttcgatca aagaacagga aacagcatta gctaatctga aacaacgcca
    21901 agctgaactt acacgagaaa tagagcaagg agctacggtt tatgaggatg cgtttgccgg
    21961 cactgtgcgt gttacgatga gtgcggaaga gcttgcgaaa aaacaagaag aattaacatt
    22021 agttactgct gatgctgaaa aagctcaaaa aaccttgaat tctagcttag aggctcaaga
    22081 agccttaaaa gctcatattc cgattgctaa cctacgagag gaatttgtta aattatatcc
    22141 tcacattgat gaaagccaaa tcaaagttga tggcttaaat attgctatcg gtaattttac
    22201 cgtcaaatca ccggagatgg ttattgctac taataacatt gctaatgctc ttggtggcgt
    22261 tgccggagag gctatgagag cggctattct agtcgctaat ctgtcaagta tgaagctgga
    22321 tgctaacggt gctgcaatta tcgatcctaa acatcttgaa aaaatcgagc aaattgaacg
    22381 taacaatgcg attggttcgg ctaaaggtaa agataaaatc gcattgcaag taaaagacgc
    22441 tctgattaaa tcaggtatga aagagggcga tgccggatac gagcgactaa aagcagcata
    22501 tgagcaacaa tttacgttgc aaaatgcccc taagggcaag ggtggaaaaa ccaaagccga
    22561 aaaagaggca gaaaaagcgg ctaaaaagca acaaagttat caaaaccaag tggcggagat
    22621 gacaaatcgt ttggcaggct taaaagccaa tgcttcagat atcgcgattt tcgggcaagt
    22681 ctccgactac caagaagtgc gtaaactgac tgaagatatt gcgatcaatg ccgaaaaata
    22741 caaaggctac ggtgagcaag gtgtcgcaag gcttaaagag ttagccagtc agttagagtc
    22801 cgcccagcaa caagtcgcta tttctcaatt tgcctacaat ggcggtgaga aactcaaagc
    22861 aatggagttt gaattaacct tgctcggtaa aacccgccaa gagcaagagt taatgcagta
    22921 caaccacgaa ttagatcttg aagcagctcg gctcaaaatc ggtatggctg atgaaaatat
    22981 tgcgaagtta gaccaggaaa tcgttaagct aaaagaacgt cgagcggaaa tccaagcaca
    23041 agcagaacaa gctcgtggca gctttaaaca aggtatgtta gatggtttga ataatatcga
    23101 agctgatgtc agcaatgtag cggcaaatgt tgccaatatt actcaaaata cctttgacgg
    23161 tatggcggat agcttgacta attttgtgat gacaggtaaa gcggatttcc gtagttttgc
    23221 taattccata ttatctgatc ttagcaaaat gattatcaaa atgatgttat ttaatgctat
    23281 caaacaaggt gcaactatgt tgtttggcta ccaaggaccg actacaacgg taaatggtaa
    23341 tgctgtttat gggttaggta cttgggcaac aggtggctac accggcgatg gtggtaaata
    23401 caccccagcg ggtatcgtcc acaaagggga atacgtcatc accaaagagg caaccgctcg
    23461 tcttggtttg gattacctaa actatcttaa ctatggcaaa cgagggtttt ccagcggtgg
    23521 tggcgttgct gtgccgagag tgccatcctc atcttatcaa cctaaatcag ctcaaagtaa
    23581 tatcagtgtt caggtgatta ataacggaga gccggttgat gcaaaagtca gccagaaaca
    23641 gcaaggcgat caaatgcaaa ttaccgttga gttaatgcgt gaaattgccc gacaggaggc
    23701 aaacggtatg atccaaaaca acttcagagc tggaggagta tttgcctaat ggaaacatta
    23761 aaatggtgtg ttcgcaccga tttagcggtt gaaaataccc ctgaaataat ggaagtcaaa
    23821 ttcggcgatg gttataccca acgctcgccc aaaggcttaa acaatttatt acgagcctat
    23881 acaggcacaa tcaaagtgaa aaagggggaa catttagcgg tggaggcatt ttttgccaaa
    23941 catcgtggtg tttccccttt ttactttaaa gatccgtaca cacaacaaaa caaaaaggta
    24001 gtgtgtagca gttggcccgc aaaaatggtt ggcttaaacc attgggaatt tagtgtaacc
    24061 tttaaggaag taccctaatg cctgttagca tatccaatca aatgaaactc gaccttgcca
    24121 aacttgagca aaatgcaatg cttgatttgt acgaggtgga cttacgcaca ttaaaggata
    24181 aaagcggtaa tgctggttc
//
LOCUS       DANLRK010000014        22480 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.014, whole genome shotgun sequence.
ACCESSION   DANLRK010000014 DANLRK010000000
VERSION     DANLRK010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 22480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22480
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.014"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(34..585)
                     /locus_tag="PXA33_000426"
     CDS             complement(34..585)
                     /locus_tag="PXA33_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5119796.1"
                     /translation="MKGIMKSILVFSTAVFLAACSSNKSGWTRLNDNDVDQKSYAIAY
                     GATAQTYADRVNESYDINSFMNGVNDWYTKKVTLPIEQIRAITINRMLDHNVYAYYSG
                     VIYAADLQANFNHLDPNCWGLVQPASMTQGIHDAMLDLQKNKVRGDEYIKNGVEQILH
                     LCVKTIAEDENESQSKGKTKKHK"
     gene            678..1529
                     /locus_tag="PXA33_000427"
     CDS             678..1529
                     /locus_tag="PXA33_000427"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005701564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HDL5119797.1"
                     /translation="MKQYLDLCNRIVSEGKWVANERTGKHCLTVINADLTYDVEKGEF
                     PLVTTRRSYWKAAIAELLGYIRGYSNAADFRQLGTKSWDANANENAQWLANPYRKGED
                     DMGLVYGAVGRNFPKPDGGSVDLLRQIVDDLKKGIDNRGEIYTFYHPGAFHMGCLRPC
                     LHSHHFSLLDGTLYLNSTQRSADVPLGLNWNMIQCYTFLALMAQITGHKAGQAFHKIV
                     NAHIYEDQLELMRDVQLKREPLNAPKLIINPDIKSLEDLETWVSLDDFKVEGYEYHPA
                     IQYPFSV"
     gene            complement(1583..2026)
                     /gene="lysM"
                     /locus_tag="PXA33_000428"
     CDS             complement(1583..2026)
                     /gene="lysM"
                     /locus_tag="PXA33_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein LysM"
                     /protein_id="HDL5119798.1"
                     /translation="MGLFDFVGDIGRKLFNKEEDASKAVTEHLAEDNPGVENVNVTVE
                     NGVAKISGIASTAAAVEKAVLMAGNVAGITEVNIDEVTLPDDVQLASDDELYVIQKGD
                     TLWEIATKAYGNGAKYKAIVEANKEVIKDENKIFPGQKIRIPKGL"
     gene            2265..2702
                     /locus_tag="PXA33_000429"
     CDS             2265..2702
                     /locus_tag="PXA33_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HDL5119799.1"
                     /translation="MKQVQFYLLSQADAAELSAAEMQACELAAQAWRLGKRVLIACET
                     EQQALNIDEALWARDPDSFVPHNLSGEITTYPTPIEISWKGKRNAQRRDLLISLQTQL
                     PEFIGSFTQIIDFVPVEETAKAQARDRYKQYRQLGWQLETIQC"
     gene            2696..3139
                     /locus_tag="PXA33_000430"
     CDS             2696..3139
                     /locus_tag="PXA33_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL5119800.1"
                     /translation="MLKNLSWRAKRFDELSTQELFEIYHARTAVFVVEQNCPYQEVDN
                     KDLQAVHFFTKNAKNLTAYCRLIPANDGVHIGRVLVVKENRGSGLARELVQKAMDYCG
                     EHFPAQPIHAQAQSYLQGFYESFGFKAVSEVYLEDGIAHLDMVLE"
     gene            3136..3878
                     /locus_tag="PXA33_000431"
                     /pseudo
     CDS             3136..3878
                     /locus_tag="PXA33_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250915.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
     gene            3880..4050
                     /locus_tag="PXA33_000432"
     CDS             3880..4050
                     /locus_tag="PXA33_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5119801.1"
                     /translation="MTSREELLKKQRELDILFTAWFEEKKKHEVLTYRRENGDLIQHY
                     PDGTEKVIEYAQ"
     gene            4040..4633
                     /locus_tag="PXA33_000433"
     CDS             4040..4633
                     /locus_tag="PXA33_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5119802.1"
                     /translation="MHNNLAIFYCGTNGAGKSTLRSFNQDKVEIVIDSDNIAMQINPL
                     NPRLADIEAGRKAIELFQFAVKNQVAFSMESTLSGKSILKRMENAKAGYFKTRLNYIG
                     VDSYKINIARVKARVKAGGHFIDEETIRKRYHISRENLIEAIFINDETLIYDNSETKP
                     NLILQIADKKVIKISDKIPNWCNQLCDELVELGFKTT"
     gene            4878..5576
                     /locus_tag="PXA33_000434"
     CDS             4878..5576
                     /locus_tag="PXA33_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5119803.1"
                     /translation="MKQNRKKSKKVIGTHKKENGLKQFIKNNKIPIILISAIWSIFTT
                     DILERIANMPIIWKNFEENYLYNKKLDGLWSTNTNYIIDYKLLNIPEEQPNVIIEFMV
                     NKDDNSILGEMTSTALCELIPLTWSFRLESPKPTLLNLIFDRELKLSYLVSGGYTELS
                     TFKIKEINSNLGTLTLTTQEDKTGIFPPTIKLINFSSNIEDDIKEIEQSCLNSRKQLW
                     EKRRKMQEEQKIPN"
     gene            5693..8557
                     /locus_tag="PXA33_000435"
     CDS             5693..8557
                     /locus_tag="PXA33_000435"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012771641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HDL5119804.1"
                     /translation="MTKQFTMEDRFNPSAVEQALYQHWESQGYFKPSEDINAPSYCIA
                     IPPPNVTGSLHMGHAFQQTLMDTLIRFNRMEGNNTLWQTGTDHAGIATQMVVERKIAA
                     EEGKTRHDYGREAFINKIWDWKAYSGGTISQQMRRLGNSIDWDRERFTMDEGLSNAVK
                     EVFVRLHEEGLIYRGKRLVNWDPKLHTAISDLEVENKESKGSLWHFRYPLANGAKTAD
                     GLDYLVVATTRPETMLGDTAVAVHPEDERYQALIGKSVILPLANREIPIIADEYVDRE
                     FGTGVVKITPAHDFNDYEVGKRHGLPMVNVMTLNADIRAEAEIIGTDGKPLSDYTAPI
                     PADYQGLERFAARKKIVADFEALGLLDQIKPHDLKVPYGDRGGVPIEPMLTDQWYVSV
                     KPLAEVATKAVEDGEIQFVPKQYENLYFSWMRDIQDWCISRQLWWGHRIPAWYDEQGN
                     VYVARDEAEVRAKHNLPADLPLKQDEDVLDTWFSSGLWTFSTLGWPEQTKELKMFHPT
                     DVLITGFDIIFFWVARMIMFTMHFIKDENGKPQVPFKTVYVTGLIRDEQGQKMSKSKG
                     NVLDPIDMIDGISLDDLLEKRTGNMMQPQLAEKIAKATRKEFAEGIVAHGTDALRFTL
                     TALASNGRDINWDMKRLEGYRNFCNKLWNASRYVLTNDKLDLSEGDVEFSLADRWIES
                     QFNRTVETFRTALSQYRFDLVANAIYEFTWDQFCDWYLELTKPIFFKGTDVQRRGASR
                     TLVNVLEKLLRLIHPVMPFITEEIWQKVKGFVGIEADTIMLQKFPQFDPLAIDETAES
                     QINFIKEVIVAVRNIRAESNIAPSKGLDLIARNFSADEVSILNANEVLLKSMAKLDSV
                     KVLENGENAPLSVAKLVANGEILIPMAGFINKEAELARLTKEMDKLKGEVARIEGKLS
                     NEAFVAKAPEQVIAKEREKMQEYLSGLEKLQVQYQEIEAL"
     gene            complement(8628..9221)
                     /locus_tag="PXA33_000436"
     CDS             complement(8628..9221)
                     /locus_tag="PXA33_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase"
                     /protein_id="HDL5119805.1"
                     /translation="MLTDQDIFNRYQTKKSEFEKQADISLIGHSLFDMWGDMENGTPN
                     LAGKTVANLGLSGTSTRQYLDVITRQERIQHVGDNVFIFLGVNDIVKEPDYSPKQVLE
                     WIGEIVDSLRKINPNANFFLLEATPVNNITTTNNAAIKELNAYFEANIPSDLTYVKTW
                     EYFADKEGNLDLTLCHDGLHFTQAGYDRLEAILTKHL"
     gene            complement(9356..10810)
                     /gene="gnd"
                     /locus_tag="PXA33_000437"
     CDS             complement(9356..10810)
                     /gene="gnd"
                     /locus_tag="PXA33_000437"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005714317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HDL5119806.1"
                     /translation="MSQKGDIGVIGLAVMGQNLILNMNDNGFKVVAYNRTTSKVDEFL
                     EGAAKGTNIIGAYSLEDLAAKLEKPRKVMLMVRAGEVVDQFIEALLPHLEEGDIIIDG
                     GNSNYPDTNRRVKALAEKGIRFIGSGVSGGEEGARHGPSIMPGGDESAWQYVKPIFQA
                     ISAKTDKGEPCCDWVGRDGAGHFVKMVHNGIEYGDMQLICEAYQFLKDGLGLSYDEMH
                     EIFKEWKNTELDSYLVDITTDILAYKDEDGEPLVEKILDTAGQKGTGKWTGINALDFG
                     IPLTLITESVFARCVSAFKDQRVAASRLFNKEIGKVEGDKKVWVEAVRRALLASKIIS
                     YAQGFMLIREASEQFGWDINYGNTALLWREGCIIRSRFLGNIRDAYEANPYLVFLGSD
                     PYFKDILENCLADWRKVVAKSVEIGLPVPCMASAITFLDGYTSARLPANLLQAQRDYF
                     GAHTYERTDKARGEFFHTNWTGRGGNTASTTYDV"
     gene            complement(10893..11582)
                     /locus_tag="PXA33_000438"
                     /pseudo
     CDS             complement(10893..11582)
                     /locus_tag="PXA33_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249555.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
     gene            complement(11592..12479)
                     /locus_tag="PXA33_000439"
     CDS             complement(11592..12479)
                     /locus_tag="PXA33_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HDL5119807.1"
                     /translation="MNPIQLIEPNINSKIFTIRGEQVMLDVHLAELYQVDTRTFNQAV
                     KRNEERFPDIFRFQLTENEWLSLRSQFVILNENSGRGQHRKYLPYVFTEQGIAMLSAV
                     LRSEVAVQVSIKIMQAFVQMRHLLQTNSGLVQRLESVERKQLEMSGQLEQVFQSLEGN
                     KPKMQGVFFEGETFNAYQFVTELVRGAKSSLILIDNYVDDTVLTLFTKRSHNVSVTIY
                     TKQISKQLALDLEKYNSQYAHINVKIFDKSHERFLIIDEKELYHIGASLKDLGKKWFA
                     FSKMDLSMSRILEKLAENQ"
     gene            complement(12655..13353)
                     /gene="pgl"
                     /locus_tag="PXA33_000440"
     CDS             complement(12655..13353)
                     /gene="pgl"
                     /locus_tag="PXA33_000440"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005620404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HDL5119808.1"
                     /translation="MNKVIFPTAQAAVEQIAKEFIEYSHQNRPVHISLSGGSTPKLLF
                     KTLAQAPFNTEIQWQNLHFWWGDDRMVAPTDPESNYGEVQKLLFDHIDIPSENIHRIR
                     GEENVEQELARFSAELTACVPNLAFDWIILGMGTDGHTASLFPHQTDFNDQNVAVIAK
                     YPETGQIRISKTASLIEKAKRITYLVTGASKAEILKEIQTTPAENLPYPAAKIQAENG
                     VTEWYLDSEAANLL"
     gene            complement(13493..14980)
                     /locus_tag="PXA33_000441"
     CDS             complement(13493..14980)
                     /locus_tag="PXA33_000441"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HDL5119809.1"
                     /translation="MNAENSCIVIFGASGDLTFRKLIPALYNLYKIGRLGEHFSVLGV
                     SRSELTDESFRQKMRDALVKFEKASGEELDKFCEHLYYQAVNTSDAVDYAKLLPRLDE
                     LHDKYQTAGNTLYYLSTPPSLYGVIPECLAAHGLTTEEFGWKRIIVEKPFGYDIETAK
                     KLDVQIHKCFEEHQIYRIDHYLGKETVQNLLVLRFSNGLFEPLWNRNFIDYVEITGAE
                     SIGVEDRGGYYDGSGAMRDMFQNHLLQVLAMVAMEPPAIINANSMRDEVAKVLYCLHP
                     LTEEDVKHNVVLGQYARGTVDGKEVPAYLEEKGVPADSNTETFMAVKCKIDNWRWAGV
                     PFYVRTGKRLPTRVTEIVIHFKTTPHPVFSQNAPENKLIIRVQPDEGISMRFGLKKPG
                     AGFEAKEVSMDFRYSDLSSSSSLLTAYERLLLDALKGDATLFARTDAVHACWKFVQPI
                     LAYKANRGRVYEYESGTWGPTEADKLIARHGKVWRKPSGTMKKKV"
     gene            complement(15055..15873)
                     /gene="cysQ"
                     /locus_tag="PXA33_000442"
     CDS             complement(15055..15873)
                     /gene="cysQ"
                     /locus_tag="PXA33_000442"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HDL5119810.1"
                     /translation="MQTLTQPLLENIINIAKAAGEQLKQLYARSVAIKIKADCTPVTE
                     ADLIISQFLTERLKQLTPDIPVLSEEFCDIPFAERQQWQEYWIIDPIDGTQQFINRTG
                     QFSVVIALVQKREGRNQPVLGVIHAPILGKTYFSMEGSGSFLQENGEIRPLVKPKRAE
                     NALLIVTVGSDLHAEQAKNAISAPQIECVKYGSGSLKAGLVAEGKADCYIRFGDTGEW
                     DTAVAEVLLSEMGGKIFDFNFSPLTYNQRESLINPYFVMVADGSIDWDKIFLFK"
     gene            complement(15887..16429)
                     /gene="nudE"
                     /locus_tag="PXA33_000443"
     CDS             complement(15887..16429)
                     /gene="nudE"
                     /locus_tag="PXA33_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HDL5119811.1"
                     /translation="MIKQLPEILNIKTVAKSRIFEVQAVDLRFSNGEERTYERLTPNR
                     RSSVMVIPIQNNELIFVKEYAVGSERYELGFPKGIVDAGEEPIESANRELQEEIGFGA
                     KKLDFLRSLYSSPSHMYGLMHLFLAQDLYPSKLEGDEPEPLEIVRYPLDKIDQLLADP
                     DFAEARNLSALFMLREYLKK"
     gene            16517..16933
                     /gene="hslR"
                     /locus_tag="PXA33_000444"
     CDS             16517..16933
                     /gene="hslR"
                     /locus_tag="PXA33_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HDL5119812.1"
                     /translation="MKNKHEKEQNDAEVRLDKWLWAARFYKTRSIAKAMIEGGKVHYN
                     GQRAKVSKAVEIGAIIKLRQGNEEKEVEVLALSDQRRGAPEAQLLYSETEKSIKHREA
                     MAFARKANALSMPHPDRRPNKKERRDLIKFKEQEFS"
     gene            17177..18070
                     /gene="hslO"
                     /locus_tag="PXA33_000445"
     CDS             17177..18070
                     /gene="hslO"
                     /locus_tag="PXA33_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HDL5119813.1"
                     /translation="MNYTKDNDKLYRYLFKDRAVRGEWVRLNNTFTETLNTHHYPKAV
                     QNLLGEMLVATSLLTATMKFNGNITVQIQGDSPLKLAVVNGNDQQQLRALARVQADIA
                     DTASLAEMIGNGVLVISIIPTDGERYQGVIALDKPTIRECLEDYFARSEQLQTHLVIR
                     TGEFEGKAVAGGMLLQIMPDGTGSRDDFEHLATLTETVKDEELFGLEAEELLYRLYHE
                     EQVEVYPPQDTEFKCGCSRERSGSALLLVPAEEIEEMLEEKQGVIDMQCECCGTQYFF
                     DKAAIEGFKEETEKLSKLGLN"
     gene            complement(18144..18782)
                     /locus_tag="PXA33_000446"
     CDS             complement(18144..18782)
                     /locus_tag="PXA33_000446"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015431925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HDL5119814.1"
                     /translation="MQNKIDWQKVDGLLPVIVQNAQTCEVLMLGYMNQQALEKTLAEK
                     RVTFYSRTKQRLWTKGETSGNFLNVVDMSLDCDNDTLLILANPIGETCHTGAESCFHQ
                     FEQEQPDWIFFSKLERMIAERKNADPESSYTAKLYAKGTPKIVQKVGEEAVESVIAAM
                     KNDREELVGEVADLMYHLTVLLHNADLSWAEINAKLKERHKGIGLHPEGSNK"
     gene            complement(18810..19196)
                     /locus_tag="PXA33_000447"
     CDS             complement(18810..19196)
                     /locus_tag="PXA33_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL5119815.1"
                     /translation="MIKITALDHLVLTVADMQKTIDFYTTILGMQEITFGDNRKALLF
                     GTQKINLHQKGAEILPNAQNADCGTADLCLLTVTPLQEVIYVLQRHQVEILEGGIVPR
                     TGAVGKIESVYCRDPDGNLIEISLYV"
     gene            complement(19193..19966)
                     /gene="hisF"
                     /locus_tag="PXA33_000448"
     CDS             complement(19193..19966)
                     /gene="hisF"
                     /locus_tag="PXA33_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005624689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HDL5119816.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAQRYAQEGA
                     DELVFYDITASSDSRTVDKSWVERVAQVIDIPFCVAGGIKTIADAEQIFAFGADKISI
                     NSPALANPELITELAKRFGVQAVVVGIDSWFEAETGKYWVNQYTGDEKRTRQTEWQLL
                     DWVEEVQKRGAGEIVLNMMNQDGVRNGYDLVQLKKVREVCQVPLIASGGAGEMVHFRD
                     AFIEANVDGALAASVFHKQIINIGELKEYLAKEGVEVRR"
     gene            complement(19948..20697)
                     /gene="hisA"
                     /locus_tag="PXA33_000449"
     CDS             complement(19948..20697)
                     /gene="hisA"
                     /locus_tag="PXA33_000449"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006995165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HDL5119817.1"
                     /translation="MQKSIIIPALDLIDGQVVRLHQGDYAKQTTYADDPIAQFADYLA
                     QGAEQLHLVDLTGAKNPAQRQTALIGKIIRETHCKIQVGGGIRTEQDVADLLAVGANR
                     VVIGSTAVKEREMVKGWFKKYGAEKFVLALDVNIDAIGQKIIAMSGWQEASGVSLEEL
                     IEDYQTVGLQHVLCTDISRDGTLAGSNVDLYREICAKYPEIQFQSSGGIGSLADIKAL
                     CGTGVAGVIVGRALLEGKFNVAEAIECWQNG"
     gene            complement(20867..21712)
                     /locus_tag="PXA33_000450"
     CDS             complement(20867..21712)
                     /locus_tag="PXA33_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor protein"
                     /protein_id="HDL5119818.1"
                     /translation="MNEIKLFENSQIRSVWDSEKEEWFFSVVDIIEVLTQSSNPRRYW
                     SDLKRKIKDEEGGIELYEKIVQLKLKAPDGKMRETDATDLQGIFRIIQSVPSPKAEPL
                     KMWLAEVGKERIDEIIDPELTIDRALATYLKKGYSREWINQRLQAIQVRKELTDTWQD
                     HGVQVGREFAILTNEITQAWSGMTTRQYKDFKGLKKESLRDNMTTTELVLNMLAEAAT
                     KDIAQISHPQGLEENKTVAKRGGNVAKVARESLEAETGKPVITQQNAIDFAAVIEQVA
                     KQVKK"
     gene            complement(21811..22404)
                     /gene="hisH"
                     /locus_tag="PXA33_000451"
     CDS             complement(21811..22404)
                     /gene="hisH"
                     /locus_tag="PXA33_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015431920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HDL5119819.1"
                     /translation="MTNLTIIDTGCANLSSVKFAFDRLGIRAEISRDLNKIQSADKLL
                     LPGVGTAAAAMAILHDRRLIDTIRNATQPMLGICLGMQLMTDFSHEGDVATLNLMSGK
                     TERLPECGLPLPHMGWNKVQYQADHPLFHDIEQGNHFYFVHSYGVLPNENTIATCDYG
                     VPFSAAIASQNFYGVQFHPERSGEAGAKLLRNFVENV"
BASE COUNT         6479 a         5005 c         4570 g         6426 t
ORIGIN      
        1 ttttaaaaat tttaccgctt gtcatagctt aacttattta tgctttttgg ttttaccttt
       61 agattgcgat tcattttcat cttcagcaat cgtttttaca caaaggtgca atatttgctc
      121 aaccccattt ttgatatact catctccacg aactttgttc ttctgtaaat ctaacatcgc
      181 atcgtgaatg ccctgggtca tactcgctgg ttgaacaagc ccccaacaat taggatctaa
      241 atgattgaaa tttgcctgca aatctgcggc ataaatcaca ccactataat aagcatacac
      301 attatggtct aacattcgat taatcgttat tgctctaatt tgctcaattg gcaatgtaac
      361 ctttttagtg taccaatcat ttaccccatt cataaaggaa ttgatatcat agctttcatt
      421 tactctatcc gcataagtct gagccgttgc accataagca atagcataag atttctgatc
      481 aacatcatta tcatttaaac gtgtccaacc gcttttattc gatgaacaag ctgctaaaaa
      541 aacagcggtg gaaaaaacca gaatggattt cattatacct ttcatatcca taactcctta
      601 ttgtgttgtg tcataaagtt ttagctattt taccgaataa atggcaaaat tacctaaaat
      661 tttttatgag gtaaataatg aaacagtatt tagacttatg taaccgcatc gttagcgaag
      721 gcaaatgggt tgcaaatgaa cgtacaggca aacactgcct cacagtcatt aatgctgact
      781 taacttatga cgtagaaaaa ggagaatttc ctttagttac cactcgccgt agttattgga
      841 aagctgcgat tgccgaactt ttaggctata ttcgaggcta tagtaatgct gctgatttcc
      901 gtcaattagg cacaaaatcg tgggacgcta atgcaaatga aaatgcacaa tggcttgcta
      961 atccctatcg aaaaggggaa gatgatatgg gcttggttta cggcgcagta gggcgtaatt
     1021 tcccgaaacc agacggtggc tcagtcgatt tattgcgaca aattgtggac gatttgaaaa
     1081 aaggaattga taaccgtggt gaaatttata ccttctacca ccccggcgct ttccatatgg
     1141 gctgcttacg tccttgttta cacagccatc atttttctct acttgatggc acgctttacc
     1201 tgaatagtac tcagcgttcg gcagatgtac cgcttgggtt aaattggaat atgattcaat
     1261 gctatacctt tttagcttta atggctcaaa ttacagggca taaggcagga caagcgttcc
     1321 ataaaattgt caatgcgcat atttatgaag atcaactaga attaatgcgt gatgttcaac
     1381 ttaagcgaga accactcaat gcacctaaat taatcattaa tcctgatata aaatccttag
     1441 aagatttaga aacttgggtg agcttagatg atttcaaagt tgaaggttat gaataccacc
     1501 ctgccattca atatccattt tcagtctaac accacctaca aaaagagcta aactttcgac
     1561 cagcttagct ctttcaattt gattataaac ctttcgggat acgaattttt tgccctggga
     1621 agattttatt ttcgtcttta ataacttctt tatttgcttc tacaatcgct ttatattttg
     1681 caccgttacc ataagctttt gtggcgattt cccacaacgt atcccctttt tggataacat
     1741 aaagctcatc atcacttgca agttgcacgt catcaggcaa ggtcacttca tcaatattta
     1801 cctcagtgat accagccaca ttacccgcca tcaataccgc tttctcaaca gctgctgcgg
     1861 ttgaagcaat gcctgaaatt ttcgctaccc cgttttcaac cgttacgttt acattttcaa
     1921 cgcccggatt gtcttccgcc aaatgctctg taaccgcttt tgaagcatct tcttctttat
     1981 taaataattt acgaccaatg tcgccaacaa aatcaaataa gcccatagta agttccttat
     2041 aaaatagcta aatgaagtgg gtattctaca caatttccaa ctattgaaat agtgcattgt
     2101 tctcttagaa cgtctaaaag ctaattagtt cctcaaggca acgcaacaca agtggtcatt
     2161 ttttctcaat tttttgcaaa aacaaacgct tagattttat attttactca ttttcagcta
     2221 gaatattaag tttaaatatc tgattgaatt tctgggaaca ttctgtgaaa caagtacaat
     2281 tttatctatt aagccaagcc gatgcagccg agctttccgc tgctgaaatg caagcctgcg
     2341 aacttgcggc tcaagcgtgg cgattaggta aacgggtact gattgcatgc gaaaccgagc
     2401 aacaagcgtt aaatattgac gaagcacttt gggcaagaga tccggattcg ttcgtgccgc
     2461 acaatttatc gggagaaatc accacttacc caacgcctat cgaaatttcg tggaaaggta
     2521 aacgcaatgc tcaacgtcgg gatttgttga tttcactcca aacccaactc cccgaattta
     2581 tcggcagttt tacccagatt attgatttcg tgccggtgga agaaaccgcc aaagcacagg
     2641 ctcgtgaccg ctataaacaa taccgccaac ttggttggca actggaaacg atacaatgct
     2701 aaaaaacctc tcttggcgcg caaaacgctt tgatgaactc tccacccaag agttgtttga
     2761 gatttaccac gcacgtacag cggtgtttgt ggttgagcaa aactgccctt accaagaagt
     2821 cgataataaa gacctgcaag cggtgcattt ctttacaaaa aatgcaaaaa atctgaccgc
     2881 ttactgccgt ttaattccag ccaatgatgg tgtgcatata ggtagagtgt tggtggtgaa
     2941 agaaaatcgt ggctcaggct tagcaaggga attggtacaa aaagcgatgg attattgtgg
     3001 cgaacatttt cctgctcaac ccattcacgc ccaagcccaa agttacctgc aagggtttta
     3061 tgaaagtttt gggtttaaag cggtttcaga agtgtatttg gaagatggga ttgcgcattt
     3121 ggatatggtg ttagaatgaa taaattagct aaaatcgaac aattaaaaac agcactttca
     3181 caacgccgtc cattatctga aagcgaagtt caacgactgc gggaagagtt tattattgaa
     3241 agcagctata attccaatgc aattgaaggc aataccatta ctttgcgtga aacggtgctc
     3301 attttaaaag agggaatgac cattgccgaa aaaccgattc gggagcattt agatattatc
     3361 ggttttaagg atgcattcaa ctatatttat gaattagtcg ccaataaaga accattaagc
     3421 gaacgagcga ttaaagatat tcattctctg gtattaatgg gcaatgcgga aagccgaggg
     3481 gtttatcgca aaattccagt acgaatttta ggggcagaaa atgccccccc ccttatttaa
     3541 ttgccgaaga aatgcaatta ttgattgaaa aatatcaaca aaaactgacc gcacttcatc
     3601 ccatagaggc aatcagttgg ctgcatttag cctttgaaag cattcaccct tttattgatg
     3661 gaaacgggct cacagggcga ttactgatta attttgaatt attaaaacag ggctatttac
     3721 cgattgatat taaatttacc aatcgagccc aatattacca atgttttgat gatttccata
     3781 aaaatcaaca agatattagt agtttggttg aattgattag tcagtatgaa ttagctgagt
     3841 tggaaaggta tttggcgatt ctgaaacaga ggacataaaa tgacaagtcg tgaagaatta
     3901 ctgaaaaaac aacgtgaact ggatattctc tttacagcat ggtttgaaga aaagaaaaag
     3961 cacgaagtgc tgacgtatcg ccgagaaaat ggggatttaa ttcagcatta tccggacggt
     4021 acggaaaaag tgattgaata tgcacaataa cctagcaatt ttttactgtg gcacaaacgg
     4081 agccggcaaa tctactttac gttctttcaa tcaagataaa gtcgagattg tgattgattc
     4141 cgataatatt gcgatgcaga ttaacccact caatccaaga ttggccgata ttgaggcggg
     4201 cagaaaagca atcgaactat ttcagtttgc agtaaaaaat caggttgcat tttcaatgga
     4261 atcaaccctc tcgggtaaat ctattctaaa acgaatggaa aatgccaaag caggatattt
     4321 caaaactcga ttaaattata ttggtgtcga tagctataaa attaacatcg cacgggtaaa
     4381 agcaagagtg aaagccggcg gacattttat tgatgaagaa accattagaa aacgctatca
     4441 cattagccgt gaaaatttaa ttgaagcgat ttttatcaat gatgaaacac tgatttatga
     4501 taattcagaa accaagccga atttgatttt gcagattgct gataagaaag tgataaaaat
     4561 ttcagacaaa ataccgaatt ggtgtaatca gctttgtgat gaattagtgg aattagggtt
     4621 taaaaccacc tagcacgagc cgtgctaggc tcgacaatcc cagctccttt ggagctgtaa
     4681 cttagagcca caaagtggct cagctatgcg taaccccaca cggcttgtgt gtgggtgaca
     4741 agctgtcgga ttaaagaaaa actttgcaaa aaacttctcg aaacagaccg cttatgccta
     4801 tctctgatag gttcttaaat aaataagctc tcgtcgaaga cgatcaccat cggggatatt
     4861 ggaacaaaaa cacaaagatg aaacagaata ggaaaaagtc taaaaaagtt attggtactc
     4921 ataaaaaaga gaatggctta aaacagttca taaaaaataa caaaattcct attattctta
     4981 tatctgccat atggagtatc ttcacaactg atattttaga acgcatcgca aacatgccta
     5041 ttatttggaa aaatttcgag gagaattacc tatataataa aaaactggat ggtttatggt
     5101 ctacaaatac aaattacatt atcgactata aattattgaa catacctgaa gagcaaccaa
     5161 atgttatcat tgaatttatg gttaataaag atgacaattc gattttaggg gaaatgactt
     5221 caacagctct gtgtgagtta atacctctaa cttggagttt tagattggaa agccctaagc
     5281 caacattatt aaatcttatt ttcgatagag agttgaaatt gagttatctt gttagtgggg
     5341 gatataccga gctatcaaca tttaaaatta aagaaattaa cagtaatcta ggaacactaa
     5401 cattaactac gcaagaagat aaaaccggta tatttcctcc gacaatcaaa ttaatcaatt
     5461 tttcatccaa tatagaagat gatattaaag agattgagca aagctgttta aatagccgaa
     5521 agcaattatg ggaaaaacga agaaaaatgc aagaggaaca aaaaattccc aattaatctt
     5581 ttagttgacc ttaatttaaa taactaatta cctataaaag catttacggc aaagatcttt
     5641 tttataagat taagctagtg aaaaaattat taaacaagag aaaacaaaac caatgacaaa
     5701 acaattcaca atggaagacc gttttaaccc ttccgctgta gagcaagcgc tctaccaaca
     5761 ctgggaatcg caaggctatt ttaagccgag tgaagatatt aatgcaccga gctactgtat
     5821 cgctattccg ccgccgaatg tgacggggag tttgcatatg gggcacgctt tccagcaaac
     5881 cttgatggat acgctgattc gttttaaccg tatggagggc aacaataccc tttggcaaac
     5941 aggaactgac cacgcaggga ttgccactca gatggtggtg gagcgtaaaa tcgcagcgga
     6001 agaaggtaaa acccgccacg attatgggcg tgaagccttt atcaacaaaa tctgggattg
     6061 gaaagcctat tcgggcggca cgatcagcca gcaaatgcgt cgcttaggca actcgattga
     6121 ctgggatcgt gagcgtttta cgatggacga aggcttatcc aatgcggtaa aagaggtgtt
     6181 cgttcgcttg cacgaagaag gcttgattta ccgtggcaaa cgcctcgtaa actgggatcc
     6241 gaaactgcat actgcgattt ctgacttgga agtggaaaac aaagaaagca aaggctcgct
     6301 gtggcatttc cgctatccgc tggctaatgg tgcaaaaacc gcagacggct tggattactt
     6361 agtggtggca acgacccgtc ctgaaacgat gttgggcgat actgcggtgg cggttcaccc
     6421 tgaagatgag cgttatcaag ctctgattgg caaaagcgta atcctaccgc ttgccaaccg
     6481 tgaaatcccg attattgccg atgagtatgt ggatcgtgag ttcggtacgg gcgtggtaaa
     6541 aatcacccct gcccacgatt tcaacgacta cgaagtgggc aaacgccacg gcttgccgat
     6601 ggtgaatgtg atgaccttaa atgccgatat tcgtgcagag gccgaaatta tcggcacaga
     6661 cggtaaaccg ctttccgact acaccgcccc aattcctgcc gactatcaag gcttagagcg
     6721 ttttgccgcg cgtaagaaaa ttgtggcaga ttttgaagct cttgggctgt tagatcaaat
     6781 caaaccacac gacttaaaag tgccgtatgg cgaccgtggt ggcgtgccga ttgagccgat
     6841 gctgaccgac caatggtatg tgtctgtcaa acctttggcg gaagtcgcga ccaaagcggt
     6901 ggaagacggc gaaatccaat tcgtgccgaa acagtacgaa aacctgtatt tctcttggat
     6961 gcgtgatatt caagactggt gtatctctcg ccaattatgg tggggacacc gcattccagc
     7021 gtggtatgac gagcagggca atgtttatgt agcacgagat gaagcggaag tgcgtgcgaa
     7081 acacaactta cctgcggact taccattaaa gcaagatgaa gacgtactgg atacttggtt
     7141 ctcgtctggt ctatggacgt tctcaacgtt aggctggcca gaacaaacca aagaactgaa
     7201 aatgttccac ccgaccgatg tgctgattac cggttttgac atcatcttct tctgggtggc
     7261 tcgtatgatt atgttcacga tgcacttcat caaagatgaa aacggcaaac cgcaagtgcc
     7321 gttcaaaacc gtgtatgtaa caggcttgat ccgtgacgag caagggcaga aaatgtcgaa
     7381 atcgaaaggg aacgtgttag acccgattga tatgattgac ggcatcagtc tcgacgattt
     7441 gctcgaaaaa cgtacaggta atatgatgca gccacaactg gcagaaaaaa tcgctaaggc
     7501 aacccgcaaa gaatttgccg aagggattgt ggcacacggc acagacgctc tgcgtttcac
     7561 cctcaccgcc cttgccagca acggtcgaga catcaactgg gatatgaaac gtttagaagg
     7621 ctaccgcaac ttctgcaaca aactgtggaa tgcgagccgt tatgtgctga ccaatgacaa
     7681 gttggattta agcgaaggcg acgtggaatt tagccttgcc gatcgttgga ttgaaagcca
     7741 attcaaccgc acggtggaaa ctttccgcac cgcactcagc caataccgtt tcgacttagt
     7801 ggcaaatgcg atctacgaat ttacttggga tcaattctgc gactggtatt tagagctgac
     7861 caaacctatt ttcttcaaag gcacagatgt acaacgccgt ggcgcaagtc gcacgctggt
     7921 gaatgtattg gaaaaactcc tgcgtttaat ccacccagta atgccgttca tcaccgaaga
     7981 aatctggcag aaagtgaaag gctttgtagg tattgaagcc gataccatta tgttgcaaaa
     8041 attcccacaa tttgacccgc ttgccattga tgaaaccgcc gaaagtcaaa tcaactttat
     8101 caaagaagtg attgtggcgg tgcgtaacat tcgtgcggaa agcaacatcg caccaagcaa
     8161 aggtttagat ttaatcgctc gtaatttctc tgcggacgaa gtctctattc tcaacgctaa
     8221 cgaagtgttg ctcaaatcga tggcaaaact tgatagcgtg aaagtgctgg aaaatggtga
     8281 aaatgcaccg ctatcggtag cgaaactggt cgcaaatggt gagatcttga tcccaatggc
     8341 aggctttatc aacaaagaag ctgagctagc tcgcctcacc aaagagatgg ataaacttaa
     8401 aggtgaagtg gctcgcattg aaggcaaact ttcaaacgaa gcctttgtcg ccaaagcccc
     8461 agagcaagtc atcgccaaag agcgtgaaaa aatgcaagag tacctgagtg gattagagaa
     8521 attgcaggtg cagtatcaag agattgaagc actgtaatta actaacaagc ggtcaggatt
     8581 tggaaatttt ttgctaattc agaccgcttg cttattgaag cttttcatta taaatgtttg
     8641 gttaaaatcg cctctaggcg gtcatagcct gcttgggtga agtgtaatcc atcgtggcat
     8701 aaagttagat ctaaattccc ttctttgtca gcaaaatatt cccacgtttt tacataagtc
     8761 aaatcagatg gaatatttgc ctcaaaatac gcattgagct cttttattgc agcattattg
     8821 gtggtggtaa tattattaac cggtgtggct tccaatagaa aaaagttggc atttggatta
     8881 attttgcgaa gagaatccac aatttcgcca atccactcta acacttgttt tggcgagtaa
     8941 tccggctctt ttacaatgtc attaacgcct agaaaaataa atacattatc gccaacgtgt
     9001 tgaattcgct cttgtcgggt aatcacatcc aaatattggc gggtgctggt gcctgataag
     9061 cccaaattgg caactgtttt tcctgctaaa ttcggcgtgc cgttttccat atcgccccac
     9121 atatcaaaca gggaatgccc aataagggaa atatctgcct gtttttcaaa ttctgatttt
     9181 tttgtttggt agcgattaaa aatatcttga tcggtaagca taggttctcc ttaaatataa
     9241 atgaaaaatc gctccattat tatacaaact tgtaaacatt cactccaaga tattttgaag
     9301 aaaaatccaa taaaaaagcc acttaatatc gaattaagtg gctcaaaaag attaattaca
     9361 catcataagt ggtcgatgcg gtattaccgc cacgtcctgt ccagttggtg tggaagaact
     9421 caccgcgagc tttgtctgta cgctcataag tatgagcacc aaagtagtca cgttgtgctt
     9481 ggagtaagtt tgccggtaaa cgtgccgagg tgtagccatc taagaaggta atggctgacg
     9541 ccatacaagg tacaggtaaa ccgatttcca ccgattttgc cactacttta cgccaatctg
     9601 ccaagcagtt ttccaaaata tctttgaagt atgggtctga gcctaagaac actaaatatg
     9661 ggttggcttc atacgcatca cgaatgttgc ctaagaaacg gctgcggata atacaacctt
     9721 cacgccataa tagggcggta ttgccgtagt tgatgtccca accgaattgc tcagaagcct
     9781 cacgaatcaa cataaagcct tgtgcataag agatgatttt ggaagcaagt aacgcacggc
     9841 gtaccgcttc tacccatact tttttgtcgc cttcaacttt accaatttct ttgttaaaca
     9901 atcttgaagc cgcaacacgt tgatctttaa acgcagatac gcaacgggcg aataccgact
     9961 cggtaatcag tgttaatgga atgccgaaat caagggcgtt aataccagtc catttacccg
    10021 ttcctttttg acctgcggtg tcgagaattt tctctaccaa tggctcgccg tcttcgtctt
    10081 tgtaggcaag aatatcagtg gtaatatcca ccaaatagct gtctaattcg gtgtttttcc
    10141 actctttaaa gatttcgtgc atttcatcat agcttaaacc taaaccatct tttaagaatt
    10201 ggtaggcttc acaaattaac tgcatatcgc catattcgat accgttgtga accattttca
    10261 caaaatgccc tgctccgtca cgaccaaccc agtcacaaca aggctcacct ttgtcggttt
    10321 tggctgaaat tgcttggaaa atcggcttca catattgcca cgcagattca tcaccgcccg
    10381 gcataattga cggcccgtga cgagcaccct cttcgccgcc cgatacacct gagccgataa
    10441 agcgaatgcc tttttccgct aacgctttca cacggcggtt ggtgtccgga tagtttgagt
    10501 taccgccatc aatgatgatg tcgccttctt ctaaatgtgg cagaagagcc tcgataaact
    10561 gatccaccac ctcaccggca cgcaccatta gcatcacttt acgcggtttt tccaacttcg
    10621 ccgctaaatc ttctaacgag taagcaccga taatgttcgt gcctttcgcc gcaccttcta
    10681 agaactcatc cacttttgaa gtagtgcggt tgtacgccac cactttaaag ccgttgtcat
    10741 tcatattcaa aattaaattt tgacccatta cggcgagtcc aatcacgccg atatcgcctt
    10801 tttgtgacat tgtttgctcc tattgttttt gattctatgc aagcggtcga attggtaaaa
    10861 ttttttgcaa atttgaccgc ttgtaacatt tattaaccta aaataaactc caccgccatt
    10921 tggctgtgta atgctgctgt atcgaaaaac ggtaacggat tgtcgttttg gttaatcagc
    10981 aacccgattt cggtacaacc taaaatcaca ccttctgcac cttgttcact aagttgttga
    11041 atagtctgaa gataaaacgc tttggcactt tcagtaatct tgcctaaaca gagttcctcg
    11101 aaaataatgc gatgaatttc ttgttgcgtt tcctcatctg gtactattga tgtgatacca
    11161 cgctccaata aaccgtcttt gtaaaaatta tcactcatag taaattttgt gccaaggagt
    11221 gctactttct gcagaccttg agctgaaatc gcctcagcca cacagtccaa aatatgcaaa
    11281 aacggcacag agatatggct actaatttga tcggcaattt tgtgcattgt gttggttgcc
    11341 aataaaatgc cttctgctcc ggcttgttct agcaatctag cttgttcaga taaaatttca
    11401 gcggctgctt gccaatttcc ctacttttgg cattgtacaa tctcttcaaa attgacactc
    11461 gaaattaaaa tcttggcact gctatttcca cctaatactt tattcaccga gcgattaata
    11521 tctaaataat aagaaaccgt gctttccgca ctcattccac ctaaaatgcc gagtgttttc
    11581 attaatatac cttattgatt ttccgctaac ttttctaaaa tgcggctcat tgataaatcc
    11641 attttagaaa aagcgaacca tttcttacct aaatctttaa gtgatgcacc gatatgataa
    11701 agctcttttt catcaataat caaaaatcgc tcgtgagatt tatcaaatat tttcacatta
    11761 atgtgagcat attggctgtt atatttttcc aaatctaatg caagttgctt agatatttgt
    11821 ttcgtataaa tcgtaactga tacgttatgc gatcgtttcg taaataaagt taaaacggta
    11881 tcatctacat agttatcaat taaaatcagc gatgattttg cacctctcac taattccgta
    11941 acaaactgat aagcgttaaa ggtttcacct tcaaagaaaa ccccttgcat tttaggctta
    12001 ttgccttcaa gagattgaaa cacctgctct aactgcccag acatctccaa ctgtttacgc
    12061 tctacacttt ctaagcgttg taccaaacca ctatttgttt gcaaaagatg acgcatttgc
    12121 acaaaggctt gcataatctt aatgctaact tgcaccgcaa cttcactacg caatactgcc
    12181 gaaagcattg caatgccttg ctcggtaaag acataaggaa gatatttcct atgttggcca
    12241 cgcccactat tttcgtttaa gatcacaaat tgtgatctta aagatagcca ttcgttttct
    12301 gttaattgaa aacgaaatat gtcaggaaat cgctcttcat tccgttttac tgcttggtta
    12361 aaggttcgag tatctacctg ataaagctct gccaaatgaa catctagcat tacttgctcg
    12421 ccacgaatag taaaaatttt agagttaatg tttggttcaa ttaattgaat tggattcatc
    12481 tttcttcctt attcttcagt gaagatcact tttgtgatct caaaataaga aagactatgc
    12541 caatttttgg cctataccac gagcagattt ttctgctttt tcgatgaata tcacaaaaat
    12601 actttgcaac atttttctaa aatttaaccg cttgtaaagg tcactgattg tgacctagag
    12661 taagttagcc gcctcactat ccaaatacca ttccgttacc ccattttccg cctgaatttt
    12721 ggcggcagga taaggtaaat tttctgccgg agtggtttgg atttctttta aaatttccgc
    12781 tttgcttgct cctgtgacca agtaagtgat ccgtttggct ttttcaatta aagatgccgt
    12841 tttggaaatg cggatctgcc ctgtttctgg gtatttggcg atcacggcaa cgttctgatc
    12901 attaaaatct gtttgatgtg ggaacagtga ggcggtatgc ccgtctgttc ccattcctaa
    12961 aataatccaa tcaaacgcca agttcggcac acaagcggtc aattctgccg aaaatcttgc
    13021 aagttcttgt tctacattct cttcaccacg aatgcggtga atattttcgc ttggaatgtc
    13081 gatgtgatcg aacagtaatt tctgcacttc gccgtagtta ctttcgggat cggttggggc
    13141 aaccattcga tcatcccccc accagaaatg caggttttgc cattgaattt cggtattaaa
    13201 cggtgcttga gctaaggttt tgaataataa tttgggggtt gaaccaccgg aaagggaaat
    13261 atgcaccggg cggttttgat ggctgtattc aataaactct ttagcaattt gttcaaccgc
    13321 agcttgggcg gtggggaaaa taactttatt cataatttgc ctcgttagat cacccctctt
    13381 tagaaaagag gggaggggga gatttgatta cgatttcaat atttcgtatg agcgtatata
    13441 acttctgcca aatctcccct agcccctctt tgctaaagag gggaactgaa atttacactt
    13501 tcttcttcat tgtaccggac ggttttcgcc atactttgcc gtgtctggcg attaatttat
    13561 ctgcctcggt tggcccccaa gtgccggatt catattcata cacacgccca cgattagctt
    13621 tgtaggctaa aatcggttgc acaaatttcc aacaagcgtg tacagcatca gtacgggcaa
    13681 ataaggttgc atcaccttta agtgcgtcta atagtaagcg ttcataagcg gtgagtaagc
    13741 ttgatgatga acttaaatca gaatagcgga aatccattga gacttctttc gcttcaaagc
    13801 ctgctcccgg ttttttcaag ccgaaacgca ttgaaatgcc ttcgtccggt tgcacacgaa
    13861 taatcaattt attttccgga gcattttggc taaataccgg gtgtggtgta gttttaaaat
    13921 gaattacgat ttccgtcaca cgggttggta agcgtttacc ggttcgcaca tagaacggca
    13981 cacctgccca acgccagtta tcgattttac attttaccgc cataaaggtt tcagtgttgg
    14041 aatcagccgg cacgcctttt tcttccaagt aagccggcac ttctttgccg tctaccgtgc
    14101 cacgagcata ctgacctaac accacattgt gtttaacgtc ttcttcagtt aacggatgca
    14161 agcaatatag cacttttgcg acttcatcac gcattgaatt agcattaata attgccggtg
    14221 gctccattgc aaccatggct aatacttgca ataagtggtt ttggaacata tcacgcattg
    14281 cccccgagcc atcatagtaa ccgccacgat cttcaacgcc gatggattct gccccggtaa
    14341 tttccacata atcaataaaa ttacggttcc aaagcggttc aaataagccg ttagaaaaac
    14401 gtaaaacgag taagttttgt accgtttctt tacctaaata gtggtcgata cggtagattt
    14461 gatgctcttc aaagcatttg tggatttgta cgtctaattt tttggcggtt tcgatatcgt
    14521 agccgaatgg tttttcaaca ataatccgtt tccagccaaa ctcttcggta gttaagccgt
    14581 gagcggctaa acattccgga atcacgccat ataaacttgg tggcgtggag aggtaataaa
    14641 gcgtgttacc ggctgtttga tatttatcgt gtagctcatc aagacgaggc agcaacttgg
    14701 cataatccac tgcatcagag gtatttacag cttggtaata aagatgttcg caaaatttat
    14761 ctaattcttc accgcttgct ttttcaaatt tgactaacgc atcacgcatt ttttggcgga
    14821 aagattcgtc ggttaattct gaacgagaaa cacctaggac ggaaaaatgc tcgcccaatc
    14881 tgccaatctt atataagtta taaagagcag gaattaattt acgaaaagtt aagtcaccgg
    14941 aagcaccaaa aatgacgata cagctatttt cagcattcat ttatttatcc tattgctaat
    15001 tgttaaaatt ttgttgggtg caagattatc ataaaaattg ctaatcggaa ctacctattt
    15061 gaataaaaat attttatccc aatctatcga gccgtcagcc accatcacaa aataaggatt
    15121 aatcaaactt tctcgctgat tataagtcag cggcgaaaaa ttaaagtcga aaattttgcc
    15181 gcccatttca cttaacagca cttctgccac agcagtatcc cattcgccgg tgtcaccaaa
    15241 acggatataa caatcggctt tcccttctgc cactaaaccg gcttttagac tgcccgaacc
    15301 atattttaca cactcaattt gtggggctga aatggcattt ttagcttgtt ctgcatgtaa
    15361 atcgctgcct acggtaacaa tcaaaagggc attctccgca cgtttcggtt tgacaagcgg
    15421 tcgaatttct ccattttctt gcaaaaagct accgctacct tccatactaa aataggtttt
    15481 tcctaatatc ggagcatgaa taacgcccag caccggctga tttctaccct cacgtttttg
    15541 cactaacgca atcaccactg aaaattgacc ggtgcggtta ataaactgtt gtgtgccgtc
    15601 aatcgggtcg ataatccaat attcttgcca ttgttggcgt tcggcaaacg gaatatcgca
    15661 aaactcttcc gataataccg gaatatctgg tgtgagttgc ttcaagcgtt cagttaaaaa
    15721 ttggctgata atcagatccg cttccgtcac cggtgtgcaa tcggctttga ttttaattgc
    15781 caccgagcgg gcataaagct gctttaactg ctcaccggct gctttggcaa tattgataat
    15841 attctcgagt aggggttgag ttagcgtttg cataatagct ccttgcctat ttttttaagt
    15901 attctcgtag cataaataga gcggacaaat tccgtgcttc ggcaaaatca ggatctgcca
    15961 ataattgatc aattttatcc aacggataac gcacaatttc cagcggttca ggctcatcgc
    16021 cttccaattt tgatgggtac agatcttgtg ccaaaaacag gtgcattaag ccgtacatat
    16081 ggcttggcga actgtaaagc gaccgcagaa aatctaattt tttcgcccca aaaccaattt
    16141 cttcttgtaa ttcacggttc gcactctcaa tcggctcttc acctgcatcc acaatgcctt
    16201 tcggaaaacc cagctcgtaa cgctccgagc cgaccgcata ctctttcaca aaaatcagct
    16261 cgttgttttg aatcggaatc accattaccg aagaacggcg attcggcgta agtcgttcgt
    16321 aagttcgctc ttcgccatta gaaaagcgta aatcgaccgc ttgcacctca aaaatgcgag
    16381 atttcgctac tgttttaatg ttcaaaattt caggaagttg ttttatcatt ttatctcacc
    16441 tttttgagtt attttagttg aaaaatctca ctttcgctct aatatttcga ctattgtttt
    16501 gattttaagg cagaaaatga aaaataaaca cgaaaaagaa caaaacgacg ccgaagttcg
    16561 gctagataaa tggctttggg cggctcgttt ttataaaacc cgttcgattg ccaaagcaat
    16621 gattgaaggg ggtaaagtcc attataatgg gcaaagagct aaagttagca aagcggtgga
    16681 gatcggtgcc atcatcaaac ttcgccaagg caatgaagaa aaagaggtgg aagtgttagc
    16741 cctcagtgac caacgccgag gggctccgga agcccaactg ctctacagtg aaaccgaaaa
    16801 aagcattaaa caccgtgagg caatggcatt tgcccgcaaa gcgaatgctc tttcaatgcc
    16861 acaccctgat cgccgtccaa acaaaaaaga acggcgggat ttaatcaaat tcaaagagca
    16921 agaatttagc taagcccttg caatcagaaa aataatccct atattgaata tcctaagtag
    16981 gggcgaaaaa tttttcgccc ctatataatt atttcgggcg aacattgatt cgcccctaca
    17041 gcgttcgatt tgcacatttt tagccacaaa atgcaatgca ttttgaacgt tggctttgcc
    17101 aacggctccg taggagcgaa caaaattaga aataattttg tgagtaataa tcgaccgctt
    17161 gcaacgagga atcaaaatga attacaccaa agacaatgac aaactctacc gctatttatt
    17221 caaagaccga gcggtacggg gcgaatgggt gcgtttaaac aacaccttta ccgagacctt
    17281 aaacacccat cactacccta aagcagtaca aaatttactc ggagaaatgt tagtcgcaac
    17341 cagtttgctg actgcaacaa tgaaatttaa cggcaacatt actgtgcaga ttcaaggtga
    17401 cagcccgtta aaattggcgg ttgtgaacgg taacgaccaa cagcaactcc gtgctttagc
    17461 ccgtgttcag gcagatattg cagacacagc ctcgttagcg gaaatgatcg gcaatggtgt
    17521 attagtgatt tctattatcc caaccgatgg tgaacgttat caaggggtga ttgctctcga
    17581 taaaccaacc attcgtgaat gtttagaaga ttacttcgcc cgctccgagc aattacaaac
    17641 ccacctcgtt atccgcacag gcgaatttga aggcaaagca gtggcaggcg gtatgttgct
    17701 acaaattatg ccggacggca caggctcaag agatgatttt gagcatttag ccacgctcac
    17761 tgaaaccgta aaagatgaag agttatttgg tttagaggca gaagaattgc tctatcgcct
    17821 ctaccacgaa gagcaagtag aagtttaccc accacaagat actgaattta aatgtggctg
    17881 ctcaagagag cgttcaggct ccgcattatt actggtgccg gcagaagaaa tcgaagaaat
    17941 gttggaggaa aaacaaggcg tgatcgatat gcaatgtgaa tgctgcggga cacaatattt
    18001 ctttgacaaa gcggcgattg aaggctttaa ggaagaaact gaaaaattaa gtaagttagg
    18061 gttaaattaa gctaaacaag cggtcaagtt tcttgtcttt tttgcaattt gtaaaaaaac
    18121 ggtgaaattt gaccgcttgc attttactta tttgagcctt ctgggtgtaa cccaatccct
    18181 ttgtggcgtt cttttagttt agcattgatc tctgcccacg agagatcggc attgtgcaat
    18241 aacaccgtta agtgatacat cagatcagcc acttcaccga ccaactcttc ccgatcattt
    18301 ttcattgccg caatcacgct ttctactgcc tcttccccta ctttttggac gatttttggc
    18361 gtgcctttgg cgtaaagttt agcggtataa gaactttccg gatcagcatt tttacgttcg
    18421 gctatcattc gttctaattt gctgaaaaag atccaatctg gttgttcttg ttcaaactga
    18481 tgaaaacagc tctccgcccc cgtatggcag gtttcgccga tagggttagc gaggattaac
    18541 agggtgtcgt tatcacaatc caaactcata tccaccacgt ttaggaaatt gcccgaggtt
    18601 tcgcctttcg tccacagccg ttgtttagta cgggagtaaa aagtcactcg cttttcagcc
    18661 agcgtttttt cgagggcttg ttggttcata tatcctaaca tcagtacttc gcaggtttgg
    18721 gcgttttgga cgataacggg cagtaagccg tccacttttt gccagtctat tttattttgc
    18781 attttttctt cctttttttg accgcttgtt tagacatata ggcttatttc aatcaaatta
    18841 ccatcagggt cacggcaata aaccgactca atttttccca ctgcccccgt acgaggcaca
    18901 atcccacctt ccaaaatctc cacctgatgt cgctgtaaca catagataac ctcctgaagt
    18961 ggagttaccg ttaagaggca taaatctgcc gtgccacaat cggcattttg ggcattgggt
    19021 aggatttctg cacctttttg gtggaggttg attttctgtg tgccaaacaa aagcgctttg
    19081 cgattatcgc caaaggtgat ttcttgcata cccaaaatgg tcgtataaaa atcaatggtt
    19141 ttttgcatat cggcaacggt taaaaccaga tgatctaagg cagtaatctt aatcatctac
    19201 gcacctcaac cccttccttc gccaaatatt ccttcaactc cccaatattg ataatttgtt
    19261 tatgaaacac actcgctgcc aacgcaccat ccacatttgc ctcaataaaa gcatcacgga
    19321 aatgcaccat ttcgcctgca ccgcctgagg cgatgagtgg gacttggcaa acttcacgca
    19381 cttttttcag ttgcaccaaa tcgtagccgt tgcgtacgcc gtcttggttc atcatattca
    19441 gcacgatttc gcctgctcct cgcttttgca cttcctccac ccaatccaac aattgccatt
    19501 cggtttggcg ggtgcgtttt tcatcgccgg tatattgatt gacccaatat ttgccggttt
    19561 ctgcctcgaa ccagctatca ataccaacca ccaccgcctg cacgccaaag cgctttgcca
    19621 gttcggtaat cagctcgggg ttggcaaggg ctggggaatt gatggagatt ttgtccgctc
    19681 cgaaagcgaa aatctgctcc gcatcggcaa tggttttgat tccgcctgcc acacagaacg
    19741 gaatatcaat cacctgtgcc acacgttcca cccagctttt atccaccgtt ctgctgtcac
    19801 ttgaggcggt aatatcgtaa aacaccaact catccgcccc ctcttgggca taacgctggg
    19861 cgagtggcac aatgtcgccg atgatttcgt ggttgcggaa ctgcacgcct ttcacaactt
    19921 gcccgtcacg cacgtctaaa caaggaatta tccgttttgc caacattcga ttgcctccgc
    19981 tacattaaat ttaccttcca acaacgcacg accgacaatc acgccagcca cgcccgtacc
    20041 acaaagcgct ttgatgtcgg caagcgagcc gataccgcct gaggactgaa attgaatctc
    20101 tgggtatttg gcacagattt ctcgatagag atctacattt gaacctgcca gcgtgccgtc
    20161 tcgggaaatg tcggtgcata aaacgtgttg caagcccacg gtttgataat cttcaatcaa
    20221 ctcctcgagc gacacaccac ttgcctcttg ccagccgctc atcgcaatga ttttttgacc
    20281 gattgcatca atgttcacat ccagtgccag cacaaatttc tccgcaccgt actttttgaa
    20341 ccagccttta accatttcac gctcttttac cgcagtcgaa ccaatcacca cacggtttgc
    20401 tccaacagca agtaaatccg ccacatcctg ctcggtgcgg ataccgccgc ccacttgaat
    20461 tttgcagtgg gtttcacgaa taattttgcc gataagtgcg gtctgtcttt gagccggatt
    20521 tttcgcccct gtaagatcga ccaaatgtaa ctgctccgcc ccttgtgcca agtagtcggc
    20581 aaattgggca atcgggtcat cggcataagt cgtctgtttc gcataatcgc cctgatgtag
    20641 gcgaactact tgcccatcga taaggtcgag ggcggggata atgatggatt tttgcatatt
    20701 attgttcctg tgtgcctagc acgagccgtg ctaggctgaa taatcccagt cgcgctgcga
    20761 ctgcctctct aagctacgga gtagcttggt ttatctagcc ccatacggct cgtatggggt
    20821 acaagcggtc aaattcttcc aaaaacttgc aaaaaaccga ccgcacttat ttcttcacct
    20881 gtttcgccac ttgttcaatc accgcagcga aatcaatcgc attttgttgc gtaatcaccg
    20941 gtttgccggt ctctgcctcc aaactttccc gagccacttt tgcaacattt ccgccccgtt
    21001 tggcaacggt tttattttct tccaagccct gtgggtggct gatttgagcg atgtctttgg
    21061 tggcagcctc agcaagcata ttcagcacca actcggtggt cgtcatattg tctcgcagac
    21121 tttccttttt cagccctttg aaatccttat attggcgagt agtcataccg ctccacgcct
    21181 gtgtgatttc attggtgaga atcgcaaatt ctctgcccac ttgcacaccg tgatcttgcc
    21241 aagtatccgt cagttctttt cgcacctgaa tggcttgcaa gcgttggtta atccactcac
    21301 gggaatatcc ctttttcaag taagtagcca aagctcggtc aatggtcaat tcaggatcga
    21361 taatctcatc aatccgctct ttgcctacct ctgccagcca cattttcaac ggctcggctt
    21421 tcgggcttgg cacagactga ataatacgga aaatgccttg taaatccgtg gcatcggttt
    21481 cccgcatttt tccgtctggg gcttttaatt tcaactgtac gattttttcg tacagttcaa
    21541 ttccgccctc ttcgtccttg attttccgct ttaaatcact ccaataacgg cgtggattgc
    21601 tactttgcgt cagcacctcg ataatgtcca ccaccgagaa aaaccactct tctttttcgc
    21661 tatcccacac cgagcggatt tggctgtttt caaatagttt aatttcgttc atttctcacc
    21721 ttctttcact ggttaaatta acagtaattt tgtcgaaata aagcgagaaa tcaagcccta
    21781 ttttcaaact tttcgatcga gatcacaaaa tcagacattc tccacaaaat tcctgagcaa
    21841 cttcgcccca gcctcgcccg aacgctcggg gtggaactgt acgccgtaga agttttggct
    21901 ggcaatggca gcggaaaacg gcacgccgta gtcgcaggtg gcgatggtgt tctcattcgg
    21961 tagcacaccg tagctatgca caaaatagaa atgattgcct tgctcaatgt cgtgaaatag
    22021 cggatggtcg gcttggtatt gcaccttgtt ccagcccata tgcggcaacg gcaaaccgca
    22081 ttcaggcaag cgttcggttt tgccactcat taagttcagt gttgccacat cgccttcgtg
    22141 ggaaaagtcc gtcattaact gcattcctaa gcagatgcca agcatcggct gggtggcgtt
    22201 gcgaatggtg tcgataagtc ggcgatcgtg caagatcgcc atcgccgccg ctgctgttcc
    22261 cacaccgggg agtagcaatt tatcggcaga ttggattttg ttgagatctc gcgagatctc
    22321 agcccgaatg ccaagacggt caaaagcaaa tttgaccgaa gacaggttgg cacagcctgt
    22381 gtcgatgatg gtgaggttgg tcatttagtt tcctgagtta agtatttctt atagtgaagt
    22441 aaaattgact caatatatct gtttccttca aaagctttct
//
LOCUS       DANLRK010000015        22080 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.015, whole genome shotgun sequence.
ACCESSION   DANLRK010000015 DANLRK010000000
VERSION     DANLRK010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 22080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22080
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.015"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            45..1067
                     /locus_tag="PXA33_000452"
     CDS             45..1067
                     /locus_tag="PXA33_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL5119820.1"
                     /translation="MANGTSRNRRSSGRVTLADVAKVVGVGQMTVSRALRTPEMVSEP
                     LRKRIEEAIIELGYIPHFSARQLASAESHNLVIVTTSISSTENTLILSALRKSVADSA
                     LQMVILIADQESWFQELINHAPQAIVFLNLDCPPNVREWIKNSNIPTVEIGAIKKEPI
                     HLSVGIDSHEAMEKMIHFLVKKGYREIGLLSAKQDLSIFKQYLESWHTTLFAHHISPL
                     KIHHVVEDISFSAGATLLTEALLNWEAIDALVFLSDELACGALYEALRRHMSIPKDLA
                     IAGLGGLEVGSISYPKLTTIAIPYEQMGKIAGKQLLTLLSGKTLSAEEMIVKLPTKLV
                     IRDSAA"
     gene            1180..1593
                     /gene="secE"
                     /locus_tag="PXA33_000453"
     CDS             1180..1593
                     /gene="secE"
                     /locus_tag="PXA33_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HDL5119821.1"
                     /translation="MATEIKTKTPEQVEQKGGKSKGANTALWLVAIVFLAVAAVGNAY
                     FSSHFSLIVRVLLLVVLLVGAVALAAITNQGQKAIHFIKESRTELRKIIWPTRPEATQ
                     TTLIVLAMCVVVSLVLWGIDSIIVALITFVTSLRF"
     gene            1595..2158
                     /gene="nusG"
                     /locus_tag="PXA33_000454"
     CDS             1595..2158
                     /gene="nusG"
                     /locus_tag="PXA33_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HDL5119822.1"
                     /translation="MSEVEVKENGSKRWYVLQAFSGFENRVAVTLREYIKLHKMEEQF
                     GEVLVPTEEVVENVGGKRRKTERKFFPGYVLVEMEMNDDTWHLVKSVPRVMGFIGGTA
                     DRPAPITKREADRILNRVQETAEKPRHRKEFQPGESILVTEGPFADFSGTVEEVDYEK
                     GRLKVSVSIFGRATPVELEFSQVEKQS"
     gene            2391..2819
                     /gene="rplK"
                     /locus_tag="PXA33_000455"
     CDS             2391..2819
                     /gene="rplK"
                     /locus_tag="PXA33_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HDL5119823.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     RTESLEKGLPIPVVITVYADKSFTFVTKTPPAAVLLKKAVGIKSGSGKPNKDKVGTVT
                     QEQIRQIAETKAADMTGATIETKMKSIAGTARSMGLIVEE"
     gene            2824..3513
                     /gene="rplA"
                     /locus_tag="PXA33_000456"
     CDS             2824..3513
                     /gene="rplA"
                     /locus_tag="PXA33_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HDL5119824.1"
                     /translation="MAKLTKKMKAIKAGVDSTKAYDINEAVEVLKKFATAKFVESVDV
                     AVNLGIDSRKSDQNVRGATVLPHGTGRTARVAVFTQGANAEAAKAAGADLVGMEDLAE
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKSGQ
                     IRYRNDKNGIIHTTIGKADFSAEQLKDNLNALLAALTKAKPTTAKGIFIKKVSISTTM
                     GAGVAVDQASL"
     gene            3575..3994
                     /locus_tag="PXA33_000457"
     CDS             3575..3994
                     /locus_tag="PXA33_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5119825.1"
                     /translation="MLRLLSFCFIFLLSACTVLPQIKQLPPQVESVKSFKVENNGQQS
                     LLLIQFYENKWRWIQTDPLGAPIARVWLTKKGWEPDGFVMPNKQAQLLFTALVNALNP
                     HSPPFYLDKGWKIEREKSQFTIWLPDSSKWKVEILGE"
     gene            4081..5303
                     /locus_tag="PXA33_000458"
                     /pseudo
     CDS             4081..5303
                     /locus_tag="PXA33_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005544283.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
     gene            5296..5700
                     /locus_tag="PXA33_000459"
     CDS             5296..5700
                     /locus_tag="PXA33_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005557532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HDL5119826.1"
                     /translation="MHKFTFPIEQIAPLVPHSGNMILLDRITAFGEDFLTAETNIRPD
                     NPLIKHGKLATYASIEIMAQAGEPVRLGYLLGTRKLHLHRQEIPIGSQLQIQIKMSIQ
                     DATGFGVFDCQLIDLADNQVLLEGALNVFSPK"
     gene            5810..7243
                     /locus_tag="PXA33_000460"
     CDS             5810..7243
                     /locus_tag="PXA33_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HDL5119827.1"
                     /translation="MQEAYDIQHLCTRKEDKSFDRKSARKEPKEILRHLIGFANADGG
                     LLVIGIEDNGEITGFNYQKAHQPEEFIHALHNLQKMPIPVTYQQIEVVNSRGLADFVL
                     LFEVTASSGRVITSHDDRVFLRSKDQTLELNFEQRLQLEYEKGQRAFEEQPIARADFT
                     DLDMDLLEQYRQKLHTDRSIEEILKARDLINKEGQITNACILLFGNNPTRYFPNARLR
                     FLRYEGKAPLTGRTFNVVKEFMLEEPIPKLLESAKAVLKSQLREFQRLDENGQFTKID
                     EYPEFAWLEGIVNAIAHRSYSNQGDCIRISMFDDRLEIFSPGGLPRPVTLENMYYTRF
                     SRNPKIARVLYEFGWVKELNEGVNRMAEEMNAYQLNTPSYFEPNENAVRLILENNIEH
                     RTLRALERIEEIFTQDIMATLNKNEVTILQYLYENEMITVKIASEILDRSKELSRRIL
                     KSLEQKNILTWHGLSPKDPTQYYTLKK"
     gene            7257..7985
                     /gene="fabG"
                     /locus_tag="PXA33_000461"
     CDS             7257..7985
                     /gene="fabG"
                     /locus_tag="PXA33_000461"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012771807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HDL5119828.1"
                     /translation="MNNTVLITGSSRGIGKAIALTLAQQGFDIVVHCRSRVEEAEAVA
                     QAVRNLGKNARVLQFDVADREKVRSILEADVEQHGAYYGVVLNAGLTRDNAFPALTDE
                     DWDSVLRTNLDGFYNVLHPIMMPMIRRRKAGRIVCITSVSGLIGNRGQVNYSASKAGI
                     IGAAKALAIELAKRKITVNCVAPGLIDTDILDENVPIEEILKMIPAGRMGDPDEVAHA
                     VAFLMDEKATYITRQVIAVNGGLC"
     gene            8048..9193
                     /locus_tag="PXA33_000462"
     CDS             8048..9193
                     /locus_tag="PXA33_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014167571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HDL5119829.1"
                     /translation="MKRVVITGIGVVTAFGKEWAEIHGYQPPKHWNRKQLRSLGRVSQ
                     FVVEASERALAQAGLLDENGVIFDWLKDRRMGVACGSSTGSTNDIKDAAELLMTGKSD
                     GFNANTYVRMMPHTTAANIGIFFGLTGRIIPTSSACSSGSQGIGYAYEAIKYGMIPMM
                     LAGGGEEFCASKVYVFDSLYAASRRNDNPKGTPRPYDKDRDGLVIGEGAGMFVLEELE
                     HALARGANIIAEVVGYGANSDGSHVTRPQATTMQRCMELALKDAGIQPNQVGYVNGHG
                     TATEQGDIAETQATTAVFGKIPLSSQKSYLGHTLGACGALESWFSIEMMRDGWFAPTL
                     NLENVDERCGDLDYLQGDGREIHTDYVMNNNFAFGGVNTSLIFKRWK"
     gene            9207..10112
                     /locus_tag="PXA33_000463"
                     /pseudo
     CDS             9207..10112
                     /locus_tag="PXA33_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251927.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dialkylresorcinol condensing enzyme"
     gene            10135..11277
                     /locus_tag="PXA33_000464"
     CDS             10135..11277
                     /locus_tag="PXA33_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HDL5119830.1"
                     /translation="MLSNVYITNTSAFLPNAPVSNDAMEAVLGMVGETPSRVRKMILR
                     SNGIQHRYYAIDPITRQATHSSTELAVQAVKKLGNSATSATCLAVGTSYPDQIMPGQG
                     VMVHGLLDAEPMEVLTTAGVCVAGMAAMKHAYNAVRIGEHRAAIATASETASAIMRSE
                     HFRAEIDHKKLDDARPEIGFEKDFLRWMLSDGAGAVLLANQPNEKGLSLKIHWIDLVS
                     YANEMPVCMYAGAEFIDGNYTGWKAMSEAQRAECSAMAVKQDVKLLNENIVKYTVEKA
                     LERIIPKYKLNAEEIDYFLPHYSSGSFRDKLLEGLQRINFLIPQEKWFTNLTTKGNTG
                     SASIYIMLDEFIRTHDLKQGQKILCYVPESGRFSSCFMLLEAVVNS"
     gene            11326..11541
                     /locus_tag="PXA33_000465"
     CDS             11326..11541
                     /locus_tag="PXA33_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDL5119831.1"
                     /translation="MDINDIPQDNSKIFNGERKVIYARNPQPEIRQMISSILACYAWD
                     ENNPFVAKSDARLKALAEVVGKAEIGD"
     gene            11822..12313
                     /gene="rplJ"
                     /locus_tag="PXA33_000466"
     CDS             11822..12313
                     /gene="rplJ"
                     /locus_tag="PXA33_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005694693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HDL5119832.1"
                     /translation="MALNLQDKQAIVAEVNEAAKGALSAVVADSRGVTVEKMTELRKA
                     AREAGVTMQVVRNTLLRRAVEGTEFECLTDTFTGPTLIAFSNEHPGAAARLFTEFAKT
                     NKEFELKGAAFEGKKQDVAFLATLPTYEEAIARLMGTMKEAAAGKLVRTLAALRDQME
                     AAA"
     gene            12372..12740
                     /gene="rplL"
                     /locus_tag="PXA33_000467"
     CDS             12372..12740
                     /gene="rplL"
                     /locus_tag="PXA33_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HDL5119833.1"
                     /translation="MSLTNEQIIEAIASKSVSEIVELITAMEEKFGVSAAAAVAAAPA
                     AGAAAAEEKTEFDVVLAEAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGISK
                     AEAEALKKELEEAGAKVEIK"
     gene            complement(12847..13677)
                     /locus_tag="PXA33_000468"
     CDS             complement(12847..13677)
                     /locus_tag="PXA33_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL5119834.1"
                     /translation="MVQKPLNHYLEMQEHFLYVPYLHHHRRIRVLLPKDYHNEGWERY
                     PVLYMHDGQNVFYSKESYSGHSWKIIPTIKSHQELPKMIIVGIDNAGANRLNEYGPWK
                     TTTGSTPETRNAGGLGEEYAKWVVETVKPFIDIHYRTKPQQEHTLLAGSSMGGIITAY
                     MGSRYPHIFGHLGVFSLASWFSEPDFLRFTHQYPLQPNTKVFIQVGTNEGDEIDSHFI
                     SNTNQTYIDCSLNYYQALIRIGVPLDNIRLRIMANEIHHEMHWADHFVEFLHFSLLRK
                     "
     gene            13903..15444
                     /locus_tag="PXA33_000469"
     CDS             13903..15444
                     /locus_tag="PXA33_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HDL5119835.1"
                     /translation="MQASLQDILEVVKAKNLTYQQKLMSLGNIAERLFNPIELLGYTE
                     EEWEHIENQMICDLNEGYAIYRPRYILPDYNVYMQKGCQFLDLPPPTNLDEALDGLLI
                     LYSHVPSITTFPVYIGRLDQLLEPFITDEEQDYIKIKRFLNHIDKTISDSFCHANIGP
                     NDTKAGRLILKAVIELENITPNMTIRYDKTKTSREFAELAAKACLLVSKPSFANDAYY
                     IQDLGEEYGIASCYNALPECGGAYTLTRLRLGTIGRACASVEQMVNEMLPKVAKLALS
                     TMDKRHKFLVEESNFFESSFLVQEGFIKRTNFTSMIAIVGLADAVNHLLKQEGLNETF
                     GQSKRGDEIATLIMDKLQAVNDAHKGLYVERTNNQYLLHAQVGANNHAEDKMNTPAHR
                     IRVGEEPTLLAHLKQSAPFHKYFPSGTGDLFAFDQTYTDHLDAVVDIIDGSFAQGYRY
                     ITTYLKNTDLIRVTGYLVKKSEVERYRKGEAVLRDTTWYGSGTDDCAKVFDRQLRDEE
                     NVNAG"
     gene            15447..16235
                     /locus_tag="PXA33_000470"
     CDS             15447..16235
                     /locus_tag="PXA33_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S cluster-binding domain-containing
                     protein"
                     /protein_id="HDL5119836.1"
                     /translation="MAALSEIFVPLHRIIPFSNVEGQGNRTSIFLQGCKLNCLYCHNP
                     ETIPRYSKEAKQVSLQYLYDQVMEAVPFIRGVTISGGEPTIHHKKLVPLFEALREQGL
                     TCYLDSSGFFDFEATEPLINVTDKFLFDLKGDGAGLQTLCFDRKNQAGKVPEQALPNV
                     NHIKPENLARNLQNLAKLLPLDKVEEVRLVYLNEFYNAHHLLEKVADLLKPYPDVLLK
                     IIRVHTKGARDETGLARFVPTIEQTDELAAFAKRHGIQKVITIY"
     gene            16248..17505
                     /locus_tag="PXA33_000471"
                     /pseudo
     CDS             16248..17505
                     /locus_tag="PXA33_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005760733.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
     gene            complement(17584..19593)
                     /locus_tag="PXA33_000472"
     CDS             complement(17584..19593)
                     /locus_tag="PXA33_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HDL5119837.1"
                     /translation="MSAENMPSVIRFEQAVAKKDYESACTELLSILSKLDSNFGGISN
                     IELNMPEQIENLENDKAIYFCTRMAVAITRLFEDPALEISEHGAMRFLTLQRWIALIF
                     ASSPYVNADHILRTYNRNKESANPNTVDLDATLQALIKFCILYLPESNILLNLDAAWN
                     ASSDLTASLCFALQSPRFIGTSSAFAKRAAILQWFPEKLAQIENLNKLPSAISHDVYM
                     HCSYDIEANKHNVKRSLNAVIRRHLLSVGWEDRKIEQLGTRNNKPVMVVLLEHFHSSH
                     SIYRTHSTSMVAACEHFHLIGLGSDAVDEMGQQVFDEFHLLPQDGSLFDRLSFLKDIC
                     DKNNPAVFYMPSIGMDLTTIFASNTRLAPIQAVALGHPATTHSDFIEYVIVEDDYVGS
                     ESCFSEQLLRLPKDALPYVPSALAPQNVVYNLRENPEVIHIGIASTTMKLNPYFLEAL
                     KAIRDRAKVKTHFHFALGQSSGITHPYVERFIKSYLGNDATAHPHSPYDEYLNILHNC
                     DMMLNPFPFGNTNGIIDMVTLGLVGVCKTGPEVHEHIDEGLFKRLGLPNWLITQTAEE
                     YVTQAIRLAENHEERLAIRRDIIENNKLQTLFSGDPRPMGQIFLAKVQAWLADKNSKN
                     AEVEVKTKKVRKAATASQSAKKQTTSKTQTAKAEKDNTAKTETKS"
     gene            complement(19603..20940)
                     /gene="rimO"
                     /locus_tag="PXA33_000473"
     CDS             complement(19603..20940)
                     /gene="rimO"
                     /locus_tag="PXA33_000473"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HDL5119838.1"
                     /translation="MSASPNIGFISLGCPKNLVDSERILTELRADGYNIIPSYENADL
                     VIVNTCGFIDSAVQESLESIGEALEANGKVIVTGCLGAKENQIREVHPKVLEITGPHS
                     YEAVMNHVHKYVPKPEFNPYTSLVPKQGIKLTPKHYAYLKISEGCDHRCTFCIIPSMR
                     GDLASRSIVQVLDEAKRLVDAGVKELLIVSQDTSAYSLDRKKEEATKTVFWNGMPIKS
                     NLITLCEQLGSMGVWVRLHYVYPYPHVDDLIPLMAAGKILPYLDIPLQHASPKILKAM
                     KRPGSIERTLERIKKWREICPELTLRSTFIVGFPGETEEDFQLLLDFLKEAQLDRVGC
                     FKFSPVEGAVATDMPDQVPEEVKEERFHRFMQLQQEISAARLQAKIGKTVQVIIDDID
                     EEGIIGRSMADAPEIDGVVYVDNPSNQLVTVGQIISVTITHADEYDLWGILNN"
     gene            complement(21095..21787)
                     /gene="mtnN"
                     /locus_tag="PXA33_000474"
     CDS             complement(21095..21787)
                     /gene="mtnN"
                     /locus_tag="PXA33_000474"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HDL5119839.1"
                     /translation="MKIGIIGAMAQEIEILRQVMVEPKVTELAGCKIFEGKINNTRVA
                     LLQSGIGKVAAAIGTTLLIQLTQPDMIINTGSAGGLDPDLNVGDILISTEVRYHDVDV
                     TAFGYEIGQLPANPPTFSPNEQLVELAQKEAKKAGYNVVSGLICSGDAFINGNDKINQ
                     IRQNFPNVVAVEMEAAAIAQVCHGFNLPFVIVRAVSDVADKESHLSFEEFLPLAAEKS
                     STIVLAMLNNID"
     gene            complement(21845..22066)
                     /locus_tag="PXA33_000475"
     CDS             complement(21845..22066)
                     /locus_tag="PXA33_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HDL5119840.1"
                     /translation="MDNNYTLSAKITELETKVAFQELIIEELNQALIEQQFAIDKLQT
                     QIRHIAEKLKGAQLSQVASQAEETPPPHY"
BASE COUNT         6474 a         4473 c         4926 g         6207 t
ORIGIN      
        1 attaaggatt tagccctata atatggaaca attaggaaaa tagtatggca aatggaacaa
       61 gtagaaatcg aagatcttcg ggcagagtca ctttagctga tgtagccaaa gttgttggtg
      121 ttgggcagat gacggtatct cgtgctttgc gtacaccaga aatggtgtcg gagccactac
      181 gtaaacggat tgaagaggca atcattgaac taggatatat tccgcatttt tctgctcggc
      241 aattagcttc tgcagaatca cataatttgg tcattgtgac gacgtcgatt agctcaacgg
      301 aaaatacctt aattttatcg gcattacgca aaagcgtggc ggattccgca ttgcaaatgg
      361 tgattttgat cgctgatcaa gaatcttggt ttcaagaact gattaatcat gctcctcaag
      421 cgatagtgtt tttgaattta gattgccctc ctaatgtccg tgagtggatt aaaaacagca
      481 atatccctac cgtagaaata ggtgcgataa aaaaagaacc gattcatctc agtgttggaa
      541 ttgatagcca cgaagcaatg gagaaaatga tccatttttt ggtgaaaaaa ggctatcgag
      601 agattggatt gttatcggcg aagcaagatt tatccatctt caaacaatat ttagaaagtt
      661 ggcatacgac tttatttgca catcatattt ctcctttaaa aatccaccac gtagtagagg
      721 atatttcgtt ttcggcagga gcaactttgt tgactgaagc ccttctaaat tgggaagcaa
      781 tagatgcatt ggttttttta tccgatgaat tggcttgcgg agcattatat gaagcattac
      841 gccgacatat gagcattcca aaagatcttg cgattgccgg tttaggtggt ttagaagtgg
      901 ggagtatttc ctaccctaaa ttgacaacca ttgcgatccc ttacgagcaa atgggaaaaa
      961 tagcaggtaa acaactactt actcttttga gtggtaaaac cttatcagca gaagagatga
     1021 ttgttaaatt accgactaag ctagtgattc gtgatagcgc cgcataatat tgattttgca
     1081 taaaaatcta taaaagatcg tggcaagtct tgtagaaaat gatagaattt aacgctttaa
     1141 aataatttct gattttttta ttcatttttt aggttatata tggctactga gattaaaaca
     1201 aaaacccccg agcaagtcga acaaaaaggg gggaaaagca aaggtgcaaa tacagcatta
     1261 tggctggttg cgattgtatt tttagcagtt gctgcggtag gtaatgccta tttttcatct
     1321 catttttcat taattgttcg agtgctttta ttggtggtgt tgctagttgg tgctgttgca
     1381 ctagcggcaa ttacaaatca ggggcaaaag gcaattcatt ttattaaaga gtctcgaacc
     1441 gaactacgca aaattatctg gccgacacgc cctgaagcaa cacaaaccac cttgattgta
     1501 ttggcaatgt gcgtagtagt ttccttggtt ttatggggta ttgactcaat catcgtcgca
     1561 ttaattacat ttgtaacaag tttaaggttc taaaatgagt gaagtagaag tgaaagaaaa
     1621 tggctcaaaa cgctggtatg tattacaagc gttttcaggc tttgaaaacc gtgtggcagt
     1681 aacattgcgt gaatacatta agcttcacaa aatggaagag cagtttggtg aagtattagt
     1741 gccaaccgaa gaagtcgttg aaaatgtggg cggtaaacgc cgtaaaaccg agcgtaaatt
     1801 cttcccaggt tatgtattgg ttgaaatgga aatgaatgac gatacttggc atttagtgaa
     1861 aagtgtgcct cgtgtaatgg gctttattgg tggtacggca gatcgccccg caccaattac
     1921 caaacgtgaa gcagaccgca ttctaaaccg tgtacaagaa acagcggaaa aaccacgcca
     1981 cagaaaagaa ttccaaccgg gcgaaagcat tttggttaca gaaggtccgt ttgcagattt
     2041 ctccggtacg gtcgaagaag tggattacga aaaaggtcgc ttaaaagtat cggtgtcgat
     2101 ttttggtcgt gcgacaccgg tagaattaga attcagtcaa gtggaaaagc agagctaatt
     2161 tcaattaatt tcacacacaa gcggttattt ttctcaattt tttgcaaaat tcagcggaaa
     2221 aaagaccgct tgtaacaaga tttattcaac aaagcgaaag ttttgcttga aaaatagtcg
     2281 caaattcttt aaaatttgcg acctattacg agccagcctg ctgactcgta ttttttatgt
     2341 aacaggggag ctgattttca gcgttatcac ccatttagag gaaaattaaa atggcaaaaa
     2401 aagtccaagc ctacgttaaa ctgcaagttg cagcgggtat ggctaaccca tcaccaccgg
     2461 ttggtcctgc attaggtcaa caaggtgtta acattatgga attctgtaaa gcattcaacg
     2521 ctcgtactga gagcttagag aaaggtttac cgattccggt tgttatcaca gtttacgctg
     2581 ataaatcttt cacattcgtt accaaaactc caccggcggc agtgctactg aaaaaagcgg
     2641 taggtatcaa atctggttct ggtaaaccga acaaagataa agtgggtaca gtaactcaag
     2701 agcaaatccg tcaaatcgct gaaactaaag cagcagatat gactggtgcg actatcgaaa
     2761 ccaaaatgaa atcaatcgct ggtacagctc gctcaatggg cttaatcgta gaggaataat
     2821 acaatggcta aattgactaa aaaaatgaaa gcaattaaag ctggcgtaga ttctactaaa
     2881 gcttatgaca tcaacgaagc agttgaagtg ttaaaaaaat tcgcaaccgc taaattcgtt
     2941 gagagcgtgg acgttgctgt aaacttaggt atcgattctc gtaaatcaga ccaaaacgtg
     3001 cgtggtgcaa ccgtattacc acacggtaca ggccgtaccg ctcgtgttgc tgtgtttaca
     3061 caaggtgcaa acgcagaagc agcaaaagca gcgggtgctg acttagtggg tatggaagat
     3121 cttgcagagc aaatcaagaa aggcgaaatg aactttgatg ttgttatcgc ttctccagat
     3181 gcaatgcgtg ttgtaggtca attaggtcaa gtattaggtc cacgcggctt aatgccaaac
     3241 ccgaaagtag gtacagtaac accaaacgtt gcagaagcgg ttaaaaatgc gaaatctggt
     3301 cagatccgct accgtaacga caaaaacggt attatccaca ctactatcgg taaagcagac
     3361 ttctcagctg aacaattaaa agataactta aacgcattgt tagctgcttt aacaaaagca
     3421 aaaccaacaa cagcaaaagg tatcttcatc aagaaagtaa gcatctctac tacaatgggt
     3481 gctggtgttg cggttgatca agcatcactt taatttttaa tagaatcaaa agaccgtagt
     3541 gttaatactc tacggtcttt ttctttaggt acgaatgtta agattattat ctttttgctt
     3601 tatttttttg cttagtgctt gtactgtatt acctcaaatt aagcaattac cgccacaggt
     3661 tgaatctgta aaatccttta aagttgaaaa taatggccag caaagtttgc ttctaattca
     3721 attttatgaa aataaatggc gttggattca aactgatcct ttaggtgctc ctattgccag
     3781 agtatggctt actaaaaaag gttgggagcc agatggcttt gtaatgccaa ataagcaggc
     3841 acagctatta tttacagctt tagtcaatgc attaaaccct cattctccac ctttttattt
     3901 agataaaggt tggaaaattg agagggaaaa atcccaattt acgatttggc taccagatag
     3961 cagtaaatgg aaagtggaaa tattggggga ataagcgaaa acttgaacct ctatgcttga
     4021 atcacttgaa tatgaatttt ataattttag tgccattaca caataatact ggaataaata
     4081 ataatgacac tatatttatc agagcccgcc atcaccagtc cgattggata tggcatcgtg
     4141 gagcatttga atgttttaat taatgcgaaa gtatctcctt tagatacgac ttcacagcct
     4201 tatttacgac ataatatttc aaatagttca ccgaaatttc tagggcaagt ctctgctaga
     4261 ttgcgtgatt ttcctgttga actatctatt gaacaccgca gccgcaataa ccaactgcta
     4321 tggcacgccc ttgcagaaat tgagccgaaa attaccgctt gtatcgagca attcgggcgt
     4381 gagcggattg cggtgatcat cggcacgtcc actaccggcg ccgatgaaaa tattcccgtc
     4441 ttcaaagccg gtgtgcaggg cgattatgcc accgagcagt ttaatcaaca gcaaattctc
     4501 ttctctgcgc cgtctgattt cgttgccgaa gtgtatggct taacggggct gagttacggc
     4561 atttccaccg cctgcacctc agaggcaaga gcgttaattt ctgctgcccg tttactgaaa
     4621 gccggcttgt gcgatgcggt gatttgcggt ggtgtggata ccctttcgcc tttaactatc
     4681 agcggattta gctcgctgga agtgttatca aaccaacaaa ccaacccatt ttccgctaac
     4741 cgtaacggca ttaacatcgg cgaaggcgca gcggtgtttg tgatgagccg tgaaccactg
     4801 gacgaacatc gcattcagtt actgggttat ggttccagct cggacgctta ccatatgtcc
     4861 tcacctcacc ccgaagggga aggggcaatt tccgctttcc aaaatgccct gcacaatgct
     4921 gatttagacg cagccgacat cggctggatc aacctgcacg gcacagggac gcttcataac
     4981 gatcaaatgg aaagcctcgc tgtggcaaaa atcttcggta atcagaccgc ttgcaccacc
     5041 accaaaccct acaccgggca tacccttggg ctgccggtgc ggtggaagcg gcaattttgt
     5101 ggggaatgct ctgtcgccaa cacaacccga ccggcaagct ccctgcgcaa ttatgggata
     5161 aacaagcgga cgaaaacctg ccgaacattg cgatcaccga tgaacagagc cgttgggcag
     5221 caggcagacg gattggcgca agcagctctt ttgcctttgg cggcaataac agcgtactga
     5281 ttttgggaga aagcgatgca taaattcacc ttccctatcg aacaaattgc accattggtt
     5341 cctcacagtg gcaatatgat cctactcgac cgcattaccg cattcggcga ggattttttg
     5401 accgctgaaa ccaatattcg ccctgacaat ccgctgatca aacacggaaa acttgccacc
     5461 tacgccagca ttgaaattat ggcacaagcc ggcgaaccag ttcgcttagg ctacctgctc
     5521 ggcactcgca aactgcacct ccaccgccaa gaaatcccga ttggctcaca gctacaaatt
     5581 cagatcaaaa tgtctattca agatgccacc ggcttcggcg tattcgactg ccaactgatc
     5641 gatttagccg acaaccaagt gcttttagaa ggggcgctga atgtgtttag tccgaaatag
     5701 agttggtagt agtaagtagt agtaagtagt agtaagtagt agtaagtagt agttggtagt
     5761 agttggtagt agttggtagt agttggtagt agttggtagg aaattcgtta tgcaagaggc
     5821 ctatgacatt caacatcttt gtactcgcaa agaagataaa tcctttgatc gcaaaagtgc
     5881 gagaaaagaa ccgaaagaga ttttgcggca tttgattggt tttgcgaatg cagacggcgg
     5941 tttattagtt attggtattg aagataatgg cgaaattacc ggttttaact accaaaaagc
     6001 ccatcagccg gaagaattta tccacgctct gcacaattta caaaaaatgc cgataccggt
     6061 gacctatcag caaattgagg tggtaaatag tcgaggttta gcggattttg tcttattatt
     6121 tgaagttaca gcttcgagtg ggagggtgat tacctctcac gatgatcggg tttttctacg
     6181 ctcaaaagat caaactttgg aactcaattt tgaacaacgt ttgcaattag agtacgaaaa
     6241 agggcagcgt gcttttgaag agcaaccgat agcacgcgct gattttactg atctggatat
     6301 ggatttatta gaacagtatc gacagaaatt acacacagat cgctcaatag aagagattct
     6361 gaaagcccga gatttaatta acaaagaagg gcaaattacg aatgcctgta ttttgctatt
     6421 tgggaataac ccgacacgtt attttcctaa tgcccgtttg cgttttttgc ggtatgaagg
     6481 taaagcacct ctgaccggta gaacttttaa tgtcgtcaaa gaatttatgc ttgaagagcc
     6541 tattcctaag ttattggaga gtgctaaggc agtattaaaa agccaattac gagagttcca
     6601 acgtttagat gaaaacgggc aattcacaaa aattgatgag tatccagaat ttgcttggtt
     6661 agaaggtatt gtcaatgcga ttgcacaccg tagttacagt aatcaagggg actgcattcg
     6721 catttcgatg tttgatgacc gtttggaaat ttttagtccg ggtggattac ctcgccctgt
     6781 gacgcttgaa aatatgtact atacccgttt ttcacgcaat ccgaagattg cacgagtgct
     6841 gtatgaattt ggttgggtca aagagctgaa tgaaggtgtt aatcgtatgg cggaggaaat
     6901 gaacgcttac caacttaata caccttctta ctttgaaccc aatgaaaatg cggtcagact
     6961 gattttagaa aataatattg aacatcggac attaagggca ttagaacgaa tagaagaaat
     7021 ttttacgcag gatattatgg cgacattaaa taaaaatgaa gttactatcc tgcaatattt
     7081 atatgaaaat gaaatgatta ctgtaaagat tgctagtgag atattagatc ggagtaaaga
     7141 attatctcgt agaatattaa aaagtttaga acaaaaaaat attctcactt ggcacggctt
     7201 aagtccaaaa gatccaaccc aatactacac actcaagaaa tagtaggaaa tataagatga
     7261 ataatacagt tctcatcacc ggctcaagcc gtggtatcgg taaagcgatt gctttaaccc
     7321 ttgcacaaca agggtttgat attgtggtac attgccgttc tcgtgtggaa gaagcggaag
     7381 cggtggcaca agcggtcaga aatctcggaa aaaatgccag agtgttacag tttgatgtgg
     7441 cggatcgtga aaaagtgcgg tcgattttag aggcagatgt ggaacaacat ggtgcctatt
     7501 acggcgtggt gctaaatgca gggcttaccc gtgataacgc ctttcctgct ttaaccgatg
     7561 aggattggga tagcgtgttg cgtaccaatt tagacggttt ttataatgtc ctgcacccga
     7621 ttatgatgcc gatgatccgt cgccgtaaag caggcaggat tgtctgtatc acctcggttt
     7681 ccggcttgat cggcaaccgt gggcaggtca attacagcgc ttcaaaagcc ggcattattg
     7741 gtgcggcaaa agcactcgcc atcgagcttg ccaaacgtaa aatcacggta aactgcgtgg
     7801 cgcccggctt gattgatacc gatattctgg acgaaaacgt cccaatcgaa gaaattctca
     7861 aaatgatccc cgcaggacga atgggcgatc ctgatgaagt ggcacacgcc gttgcgtttt
     7921 taatggatga aaaagccact tacatcacac gccaagtcat cgctgtgaat ggtgggttgt
     7981 gttaacttta ggataataat tcgtaggggc ggatccagta cccgcccgaa gccaaaaaga
     8041 gataaatatg aaacgagtcg tcatcaccgg aattggtgtg gttaccgctt ttggtaaaga
     8101 gtgggcggaa attcacggct atcaaccgcc gaaacattgg aaccgcaagc agttgcgcag
     8161 ccttgggcga gtgtcgcaat ttgtggtgga ggccagcgag cgggctttag cacaggccgg
     8221 tttgttggac gaaaacggcg tgattttcga ctggctaaaa gacaggcgaa tgggcgtggc
     8281 gtgtggctcg tccacaggct ctaccaatga catcaaagat gccgccgaat tgctgatgac
     8341 cggcaaatcg gacggtttca atgccaacac ctatgtgcga atgatgcccc acaccaccgc
     8401 tgccaatatt ggcattttct tcgggctgac agggcggatt atcccgactt ccagcgcttg
     8461 ctcctcgggc agccaaggta tcggttacgc ttacgaagcc attaaatacg gtatgatccc
     8521 gatgatgctt gccggcggtg gagaagaatt ttgtgcctcc aaagtctatg tcttcgacag
     8581 cctctatgct gccagccgcc gcaacgacaa cccaaaaggc acgccacgcc cctatgacaa
     8641 agaccgagac ggcttagtga tcggcgaggg agcgggaatg ttcgtattgg aagagctgga
     8701 acacgcgtta gcacgagggg caaacattat cgccgaagtg gtgggctacg gtgcaaacag
     8761 tgacggttcc cacgtcaccc gcccacaagc caccacaatg caacgctgta tggaacttgc
     8821 ccttaaagat gccggtattc aaccgaacca agtcggctat gtaaacggac acggcacggc
     8881 caccgagcag ggcgacattg ccgaaactca agccaccaca gcggtgttcg gcaaaattcc
     8941 gctcagctcg caaaaaagct acttagggca tacgctcggt gcgtgtggtg cattggaaag
     9001 ctggttcagc attgaaatga tgcgagacgg ctggttcgcc ccaacactga atttagaaaa
     9061 tgtcgatgaa cgctgtggcg acttggatta ccttcaaggt gacggacggg aaatccacac
     9121 cgattatgtg atgaacaaca atttcgcctt cggcggtgtg aatacctcat tgatttttaa
     9181 acgttggaaa taaccgtttt caaataatga aaaagatatt attagtttat tactcccaaa
     9241 ccgggcaact cacccatttg gcagaaaatt ttgtacaatc gcttgaacaa gcgggcgttt
     9301 ttgtggaaaa actcgcaatc aaaccgcaac aggaataccc gtttccgtgg cggtttatgc
     9361 gtttttttaa caccttcccc gaaaccgtcc acctcacacc gccgccgatt gagcctctgc
     9421 cctttcagca cgaaatatac gatttggtga tcatcgccta ttccgtgtgg tttctctccc
     9481 ccagccagcc gattaccgct tttttacaga gtgaacaggc aaaaaagtgt tgaaggatac
     9541 gcccgtcatc acgctgatcg gctgtcgcaa tatgtggctg atggcgcagg aaaaagtgaa
     9601 aaaactgctt gccgagagtg aggcgaagct cattggcaac gtggtcaaag tggatcaatc
     9661 taacgactgg gcgagtttta tcaccacacc gctttggatg ctgaccggca agaaaaaaaa
     9721 tcggcaagct gccgagcgct ggcattgccg aaagcgaaat ggaagatgta aaacgcttcg
     9781 gggcaaaact tttgcacatt tttagccaaa accaaccgct tgacagcacc cttttccgcc
     9841 aaatgcaagc ggtcaaaatt gacgaaaaat tgatgatgag cgaaaaagtc ggtgcgagaa
     9901 gtttccacct ttggggcaaa ttactgatca agtgcggtca aatttcaccg cacttacgcc
     9961 aagcggtact ctatttttac atcctctttt tagtgacgtt aattttaacc gtcgtcccga
    10021 tttcaacggt gattaaacgg ctattacgcc cactgctgaa agaaaaatta gaaaaacaaa
    10081 aacggtattt tgccgagcct tcgggcgagt aaacgtaccc tacacggaaa ctttatgcta
    10141 tctaacgtct atatcaccaa cacctccgcc tttttaccca acgcccctgt gtccaacgat
    10201 gcaatggaag cggtgttggg aatggtgggg gaaacgcctt cccgagtgcg gaagatgatt
    10261 ttgcgttcta acggtattca acatcgttat tatgccattg acccgataac ccgccaagcg
    10321 actcacagca gtaccgagtt ggcggtgcaa gcggtcaaaa aattgggaaa ttctgcaaca
    10381 tcagcgactt gcttggcggt ggggacatct taccccgacc aaattatgcc ggggcagggg
    10441 gtgatggtac acggcttgct tgatgccgaa ccgatggaag tgctgaccac tgccggtgtg
    10501 tgcgtggcgg gaatggcagc gatgaagcac gcctataatg ccgtacgcat cggtgagcat
    10561 cgtgctgcga ttgcaacggc ttccgaaacc gcctccgcca ttatgcgcag tgaacatttc
    10621 cgtgccgaaa tcgatcataa aaaactggac gatgctcgcc ctgagatcgg ttttgagaaa
    10681 gatttcctgc gttggatgct ctcagacggt gcaggggcgg tattgcttgc caatcagccg
    10741 aacgaaaaag ggttgagcct caaaattcac tggatcgatc tggtctctta cgctaacgaa
    10801 atgccggttt gtatgtacgc cggtgcggaa tttattgacg gcaactacac cggctggaaa
    10861 gcaatgagcg aagcccaacg tgccgaatgc tcggcaatgg cggtgaaaca agatgtcaaa
    10921 ctgctcaacg aaaacatcgt caaatacacg gtggaaaaag cattggagcg gattatcccg
    10981 aaatacaagc tcaacgctga agagattgac tacttcctgc cgcactactc gtccggttcc
    11041 ttccgagata aattattgga aggtttacag cggatcaatt tcctgatccc acaggaaaaa
    11101 tggttcacca acctgactac caaaggcaac actgggtcag cctcgatcta cattatgctc
    11161 gatgaattta tccgcaccca cgatctcaaa cagggacaaa aaatcctctg ctatgtgccg
    11221 gaaagcggac gtttttcgtc ttgttttatg cttttggaag cggtggtaaa ttcgtaaaat
    11281 cgtagggacg ggggctattc ccgtccaaca atcatcggaa aacgtatgga catcaatgac
    11341 attccccaag acaacagcaa aatcttcaac ggcgaacgca aagtgatcta cgcccgcaat
    11401 ccgcaaccag aaatccgcca aatgatctcc tctattctcg cttgctacgc ttgggacgaa
    11461 aacaacccgt ttgtagcgaa gtctgatgca cggttgaagg cattggcgga agttgttggt
    11521 aaagcagaaa ttggcgatta agtttgattt tgtgctttac agggtcgcag attatctgta
    11581 taatttgcga ccttaacttg cgagagcaag ctgatggtgc ttagcctatc taagccccgt
    11641 ctaagactgt aggggtgaaa acttaataat cctacataga tggtgaacag acagaattct
    11701 ctgcttctgg acaccttagg ctcaaagaag attgcagttt gtagcaatgc aagcggtaat
    11761 atttttggga ttttttgtaa attctgcttg aaaaggcaga gtgtatcagg agctaaaacc
    11821 aatggcatta aatcttcaag acaaacaagc gattgttgct gaagtaaacg aagctgccaa
    11881 aggtgcactt tcagctgttg ttgcggattc tcgcggtgta acagttgaaa aaatgactga
    11941 gttacgtaaa gcagctcgtg aagctggtgt gacaatgcaa gttgtacgta atactttatt
    12001 acgtcgtgca gtggaaggca ccgagttcga gtgcttaaca gatacgttta ccggtccaac
    12061 tcttatcgca ttctcaaatg aacacccagg tgcagcagca cgtttattca ctgaatttgc
    12121 gaaaacaaac aaagagtttg aacttaaagg tgcagccttt gaaggtaaaa aacaagatgt
    12181 tgcattctta gcaaccttac caacttacga agaagcaatt gcacgtttaa tgggcacaat
    12241 gaaagaagct gcggcaggca aacttgttcg cactcttgca gcattacgcg atcaaatgga
    12301 agcagcagct taattttaag cactcttctt tattcgttta acaacttatt ttactttagg
    12361 aatcgattgt tatgtcatta actaacgaac aaatcattga agcgattgct tctaaatcag
    12421 tatcagaaat cgttgaatta atcactgcga tggaagaaaa attcggtgtt tcagcagcgg
    12481 cagcagtagc agcagctcca gcagcaggcg cagcggcagc agaagagaaa actgagtttg
    12541 atgtagtatt agcagaagct ggtgcaaaca aagttgctgt aatcaaagca gtacgtggtg
    12601 caacaggttt aggcttaaaa gaagctaaag acttagtaga atcagctcca gctgcattaa
    12661 aagaaggcat ctctaaagca gaagctgaag cacttaagaa agaattagaa gaagctggcg
    12721 cgaaagtaga aatcaaataa tttttgattt attttgtcag aacttcaagt tcaacaaacc
    12781 ccgatggaaa catcggggtt ttgttttttc agaattgtgt aatgtagata tgattacctt
    12841 tatccattac ttgcgcagca gtgaaaaatg taaaaattcc acaaagtgat ctgcccagtg
    12901 catttcgtgg tggatttcat ttgccataat ccgcaggcgg atattgtcta acggcacgcc
    12961 aatccgaatc agtgcttggt agtaattgag cgaacaatca atataggttt ggttcgtatt
    13021 agagataaag tgcgaatcga tttcatcgcc ttcgttagtg ccaacctgaa taaacacttt
    13081 ggtattgggt tgtaacggat attggtgcgt aaaacgcaga aaatccggct cactaaacca
    13141 agaggcaagc gaaaacaccc ctaaatgccc gaaaatatgt gggtagcggc tgcccatata
    13201 agcggtgata atgccaccca ttgaactgcc tgccaataag gtatgttctt gctgaggctt
    13261 ggtgcgatag tgaatatcaa taaagggttt aaccgtttcc accacccatt tggcatattc
    13321 ctcccctaag cctccggcat ttcgggtttc aggggttgag ccggtggttg ttttccacgg
    13381 gccgtattca tttaagcgat tagctcctgc gttatcaatg ccgacaataa tcatcttcgg
    13441 taattcttgg tgtgatttaa tggttggaat aattttccaa gaatgccccg aataagactc
    13501 tttgctgtaa aacacatttt gcccgtcgtg catatataac accggataac gttcccaacc
    13561 ttcgttgtga taatccttcg gcaatagtac ccgaatgcgg cggtgatggt gtaaataggg
    13621 aacatataaa aaatgctcct gcatttctaa atagtggtta agtggttttt gtaccatcag
    13681 tctgtccttt tgcttaaatt gtggcgtatt ttacacctta atcagtctga gttaaaacaa
    13741 gcggtcagaa aaagtcaaaa atttgcaaat tgctgttacc taacgcaatc ggttgcctta
    13801 tgcttttgag gaatataaaa gagaaactgt gatctaagtc acagcaattt ttattcggtt
    13861 tgactaaaat ccgaactatc acttaataga gaggaaaatg ttatgcaggc ttcattacaa
    13921 gatattcttg aggtagtaaa agcaaaaaat ctcacttatc agcaaaaatt gatgagttta
    13981 ggcaatattg ctgagcggtt atttaacccg attgaactac ttggctacac tgaagaagag
    14041 tgggaacaca ttgaaaatca gatgatttgc gatttaaatg aaggttatgc gatttatcgc
    14101 ccacgttata ttttgcctga ttataatgtg tatatgcaaa aaggttgcca attcttagat
    14161 ttacctccgc caaccaattt agatgaagcc ttagacggct tgttaattct gtattcacac
    14221 gttccgtcca ttactacttt tccggtgtat atcgggcgtt tagaccaact gttagaaccg
    14281 tttataaccg atgaagagca agattacatt aaaatcaagc gtttcttaaa tcatatcgat
    14341 aaaaccattt ccgattcttt ctgccacgct aacatcgggc cgaatgacac caaagccggt
    14401 cgtttaatct taaaagcggt gattgagctt gaaaacatta caccgaatat gacgattcgt
    14461 tatgataaaa ccaaaacatc gcgtgaattt gccgaactgg cggcgaaagc gtgcttgttg
    14521 gtgtccaaac cgtcttttgc taatgatgcc tactacattc aagatttagg cgaagaatac
    14581 ggaattgcaa gctgttataa cgccttaccg gaatgtggcg gtgcttacac tctgacccgc
    14641 ctgcgtttag gtactatcgg gcgtgcttgt gcgagtgttg aacaaatggt caatgagatg
    14701 ctgccgaaag tggcaaaact cgccctttcc acgatggata aacggcataa attcttggtg
    14761 gaagaaagca atttctttga aagtagcttc ttggttcagg agggctttat caaacgtact
    14821 aacttcacaa gtatgattgc gattgtaggt cttgctgatg cggtgaatca tttactcaag
    14881 caagaaggtt taaatgaaac ctttgggcaa agcaaacgtg gcgatgaaat tgcgacgctg
    14941 attatggata agctccaagc agtaaatgac gctcataaag ggctgtatgt tgaacgaacc
    15001 aataaccaat atctgttaca cgctcaagtg ggggcaaata accacgctga agacaaaatg
    15061 aacacgccgg cacaccgcat tcgtgtgggc gaagagccaa ccttattggc acacttaaaa
    15121 cagtctgctc ctttccataa atatttccca tccggtacgg gggatttatt cgcctttgac
    15181 caaacctaca ctgatcattt agatgcggtg gtggatatta ttgacggctc gttcgcccaa
    15241 ggttatcgct acattaccac ttacctgaaa aatactgatt tgattcgagt gacaggctac
    15301 ttggtgaaaa aaagcgaggt tgaacgttat cgcaaaggcg aagcggtatt gcgtgatacc
    15361 acttggtatg gttcaggcac cgatgattgt gcgaaagtgt tcgaccgcca gctgcgtgat
    15421 gaagaaaatg tgaatgcagg gtaacaatgg ctgcactttc tgagattttt gtgccgttac
    15481 accgcattat ccccttctcg aatgtagaag ggcagggcaa tcgtaccagc atttttctac
    15541 aaggttgcaa gctgaattgc ctgtattgcc ataacccgga aaccattcca cgttatagca
    15601 aggaagcaaa acaggttagc ttgcaatact tgtacgatca agtgatggag gcagttccct
    15661 ttattcgagg agtcactatt tcgggtggag agccaaccat tcatcataaa aaattagtgc
    15721 cactgttcga ggcgttgcgt gagcaagggc taacctgtta tttagacagc agcggctttt
    15781 ttgattttga ggcaacagag ccgttaatta atgttaccga taaatttctg ttcgatctca
    15841 aaggagatgg ggcgggctta caaacgctat gctttgatcg gaaaaaccaa gccggcaaag
    15901 tgcctgaaca ggctcttcca aacgtgaatc atattaaacc tgaaaacttg gctcgcaatt
    15961 tgcaaaattt agccaaatta ttaccgcttg ataaggtgga agaagtacgt ttggtttacc
    16021 tcaatgagtt ttataacgcc caccatttgc tcgaaaaagt ggctgaccta cttaaacctt
    16081 acccagatgt actacttaaa attattcggg tacacaccaa aggagcgaga gatgaaacag
    16141 gcttggctcg ttttgtaccc actatcgagc aaaccgatga actggcagca tttgcaaaac
    16201 ggcacggtat tcaaaaagtt attactattt actaatggag acttacgatg gatatactaa
    16261 ttagcataat tggtgttttt gtgttattag gcttaggtgt tttattatca aataatcgca
    16321 aagccattaa atttcgcaca attttgggcg ctttggcgat tcaaattggt tttgccgctc
    16381 ttattcttta ctttccagcg ggaagaaatg cattgctagc tactgcaaat tgtgtgagca
    16441 atattattaa ttatggtaac gaaggtatta gctttgtgtt tggcaattta gcgaatccga
    16501 gtaatagcag cattggtttt gtgtttgcgg ttaaagtatt gcctattatt atcttcttct
    16561 ctgcgttaat ttcaatgctt tactaccttg gtgtgatgca atgggtgatt aaaattattg
    16621 gtggagcatt acaaaaaaca ctaggcactt ccaaggcaga atcaatgtca gcggcagcta
    16681 attatttttg ttggtcagac agaagctcct ctagttgtaa aaccatacat tagccgtatg
    16741 acagagtcag aattatttgc cattatggtt ggtggaactg cttcaattgc tggttccgtt
    16801 atggctggtt atgccggaat gggcgttcct ttaacttatc ttattgcggc ctcctttatg
    16861 gctgcaccag ctgggttact atttgctaaa ttaatgtatc ctcaaacgga aaccttggag
    16921 agtaaagcag atgacaatgt tgaagttgaa aagccaaata acgtggttga ggccctagct
    16981 aatggtgcta gtgcaggtgt tactttggct attaatgtcg gtgctatgtt aatagcattt
    17041 attgcagtga tcgctttatt aaatggcttt attggtggca ttggtaattg gtttggttat
    17101 gaggggctaa ccttacaaat tttattaggt tatttattcc aaccacttgc tttcttaatc
    17161 ggtgtgccgt ggcaagatgc cgatattgca ggtcaaatga taggtatgaa acttgctgtt
    17221 aatgaatttg tcggatatct tgagtttgct aaatatctac agcctgatgc tgtggtgcaa
    17281 ttaagcgata aaactaaggc aataattact tttgctttat gtggttttgc aaactttaga
    17341 gcaattgcgg tattgattag tggtatcgga agtatggcac caagtcggcg ttcagatatt
    17401 gctcgtttag gtttaaaagc agtgattggc ggcacattgg cgaacttaat gagtgcaact
    17461 atagcgggtt tctttattgg tttaagtggt gccacattag gttgatacaa gcggttaaat
    17521 tgactcactt ttttgcaaaa agcccaagta agttaaatga ctacttgggc tttgtttaat
    17581 gaactagctc ttagtttcgg tttttgctgt gttatccttt tccgctttgg ctgtctgtgt
    17641 tttcgaagtt gtctgttttt ttgcggattg gctagcagta gctgcttttc ttactttctt
    17701 tgttttaact tctacttccg catttttaga gtttttatcc gctaaccatg cctgcacttt
    17761 agctaagaat atttgtccca tcgggcgagg atcgccagaa aataatgttt gtaatttatt
    17821 attttcaata atgtcgcgtc taattgctaa gcgttcttcg tggttttccg ctaaacgaat
    17881 agcttgagta acgtattcct ctgctgtttg ggtaattaac caattaggta gcccgaggcg
    17941 cttaaataaa ccttcatcaa tatgttcatg aacttcaggg cctgttttac agacaccaac
    18001 taaaccaagc gtaaccatat cgataatgcc gtttgtattt ccaaatggga atggatttag
    18061 catcatatcg caattatgta gaatatttag atattcatca tatggcgaat gtgggtgtgc
    18121 ggttgcatca tttcctaaat agcttttgat aaaacgttcc acataaggat gcgtaattcc
    18181 actagattgc cctaatgcaa aatggaaatg agtttttacc ttcgccctat cacgaatggc
    18241 ttttaatgcc tctaagaaat atgggtttaa tttcatagtg gttgatgcaa ttccgatatg
    18301 gataacctct ggattttcac gtaggttata gaccacattt tgaggcgcta atgcggaagg
    18361 cacatagggt agcgcatctt ttggaagacg taataattgt tcactaaagc agctttcgga
    18421 accaacatag tcatcttcta cgatgacata ttcaataaaa tcagagtgtg tagttgccgg
    18481 atgacctagc gcaacagctt gaattggtgc taggcgagtg ttactagcga aaatagtggt
    18541 taaatccatt ccaatgctag gcatatagaa tacggctgga ttatttttat cacagatatc
    18601 ttttaaaaag cttaagcggt caaatagtga gccatcttgt ggtaataaat gaaattcgtc
    18661 gaatacctgt tgccccattt catctacagc atcactgcct agcccaataa gatggaaatg
    18721 ttcgcatgca gcaaccattg aggttgaatg agtacgataa atagaatgtg atgaatggaa
    18781 atgctccaat aatactacca tgacaggttt attatttctg gtaccaagct gttcaatttt
    18841 tctatcttcc caaccgacag aaagtaaatg acgacgaatt acagcattta acgaccgttt
    18901 tacattatgt ttatttgctt caatatcata actgcaatgc atataaacat cgtgtgaaat
    18961 ggcgctcggt agcttgttca agttttcaat ttgcgctaat ttttctggga accattgcaa
    19021 aatagccgcg cgttttgcaa aagcagagga tgtaccaata aatctaggtg attggagtgc
    19081 aaaacaaagt gatgcggtga gatctgatga agcattccat gcagcatcta gattcaataa
    19141 aatattagat tccggtaagt ataaaataca gaacttaatt agtgcttgta atgtggcatc
    19201 taaatcaacc gtatttggat tagcagattc tttatttcta ttgtaagtac gcaagatatg
    19261 gtctgcgttt acgtatggag aactggcaaa aattaatgct atccagcgtt gtaatgtcag
    19321 aaaacgcata gcaccgtgtt cggagatctc taatgcagga tcttcaaaca agcgagtaat
    19381 cgctaccgcc attcgagtac agaaatagat agctttatca ttttctaaat tttcaatctg
    19441 ctctggcata ttcaattcaa tattggaaat gccaccaaaa ttactatcta attttgaaag
    19501 gatagacagt agctcggtac aagccgattc ataatctttt ttcgcgaccg cctgctcaaa
    19561 acggatgacg ctaggcatat tttctgctga cataatgtat ccttagttgt ttaaaattcc
    19621 ccacagatca tactcatctg catgtgtaat ggttactgaa ataatttgcc ctactgtcac
    19681 taattggttt gaaggattat ctacatagac tacaccgtca atttccggtg catctgccat
    19741 tgagcgaccg ataatgcctt cttcgtcaat gtcatcaata attacttgta cggttttgcc
    19801 gatttttgct tgtaagcgag cagcggaaat ttcttgttgt agttgcataa aacggtggaa
    19861 acgttcctct tttacttctt ccggaacttg atccggcata tcggtagcaa cagcaccttc
    19921 cactgggctg aatttaaagc aaccaacacg atctaattgt gcttctttta agaaatcgag
    19981 taatagttgg aaatcttctt ctgtttcccc cgggaagccg acaataaagg tagaacgcag
    20041 agttaattca gggcaaattt cacgccattt tttaatgcgt tctaaggttc tttcgattga
    20101 acccgggcgt ttcatcgctt ttaagatttt cgggctggcg tgttggagtg gaatatccaa
    20161 atacggcaaa attttgccgg cagccataag cggaattaaa tcatctacgt gtgggtatgg
    20221 gtaaacatag tgcaaacgca cccacacgcc cattgagcct aattgctcac atagcgtaat
    20281 taaattactt ttaatcggca taccgttcca gaaaacggtt ttggttgctt cttctttttt
    20341 gcgatctaac gaataggctg aggtgtcttg tgagacgatt aacagctctt ttaccccggc
    20401 atcaactagg cgttttgctt catctaatac ttgcacgata gaacggcttg ctaaatcgcc
    20461 acgcattgaa ggaataatgc agaaagtgca gcgatgatcg cagccttcgg aaattttcaa
    20521 ataagcgtaa tgcttcggtg ttaatttaat cccttgtttt ggcactaagc tggtgtaagg
    20581 gttaaattcc ggttttggca catatttatg gacgtggttc attaccgcct catagctatg
    20641 tggccctgta atttccaata ctttagggtg tacctcacga atttggtttt cttttgcccc
    20701 taaacagccg gtgacaatta cttttccgtt agcttctaac gcttcaccaa tgctttcaag
    20761 agattcttgt actgcggaat caataaaacc gcaggtgttt acaatcacaa gatccgcatt
    20821 ttcataacta gggatgatgt tataaccatc cgcacgcagt tcggtcaaaa tacgttcgga
    20881 atcaactaaa tttttagggc agcctaagct gataaaacca atatttggtg aagcactcat
    20941 ataactctca ataactaaaa agaaaaatgc ctacaatttt tgtctgtagg caaaaaacta
    21001 acaagattct acaaaatctt gcccaaaata gcgataacaa gcggtcaatt tttaataaac
    21061 atttacaaac ttgaaatttc gctaacaatt aaaattaatc gatattattt agcattgcta
    21121 atacaatcgt ggaagatttt tctgcggcta atggcagaaa ttcttcaaaa gaaaggtggg
    21181 attctttgtc cgctacatcc gatactgctc ttacgattac aaatggaagg ttaaaaccgt
    21241 gacaaacctg cgcaatggct gctgcttcca tttcgactgc aacgacattc gggaaattct
    21301 gacgaatttg attaatctta tcattaccgt taataaaggc atcgccgcta cagattaagc
    21361 ctgaaaccac attgtaaccg gcttttttcg cttccttttg agctaattcg actaattgct
    21421 cgtttggcga aaatgttggc gggtttgccg gtagttgccc gatttcataa ccaaaggcgg
    21481 tcacatcaac atcgtgataa cgaacttcgg ttgaaatcaa aatatcacct acgtttaaat
    21541 ctggatctaa tccaccggca gagcctgtat taataatcat atccggctgg gtgagctgta
    21601 tcagtaaggt agtgccgatg gcggcagcaa ctttgccgat acccgattgt aataatgcca
    21661 cacgggtatt gttaattttg ccttcaaaaa ttttacaacc tgctaattca gtgactttag
    21721 gttcaaccat tacttggcgt aaaatctcaa tttcttgtgc catcgcacca ataataccaa
    21781 ttttcattac tttccttctc taatgtattg tttatttttt gaaaaaaacg ctattttaac
    21841 agcattaata gtgaggaggg ggtgtttctt ctgcttgtga agcaacttgg cttagttgag
    21901 cccctttcaa tttctcagca atatggcgaa tctgagtttg taacttatca attgcaaatt
    21961 gttgctcaat aagtgcttga tttaactctt caataatcag ctcttggaaa gcaactttag
    22021 tctccaattc ggtaattttt gccgataatg tataattatt atccattttt tactaaaaaa
//
LOCUS       DANLRK010000016        21479 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.016, whole genome shotgun sequence.
ACCESSION   DANLRK010000016 DANLRK010000000
VERSION     DANLRK010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 21479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21479
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.016"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(<1..411)
                     /locus_tag="PXA33_000476"
     CDS             complement(<1..411)
                     /locus_tag="PXA33_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5119841.1"
                     /translation="MIQNDPRDAIILSLLANAVYGGHNNSQISYQETEEVNLYARLRQ
                     VEGDVREIKSNYLTKEAFYKAGGSTLIALVVTAVIALWTVYSHLETKVEARFSKIDDK
                     FVQVETNIKNLDIRLTKVESRLDNVEQRLDNVEQR"
     gene            complement(416..796)
                     /locus_tag="PXA33_000477"
     CDS             complement(416..796)
                     /locus_tag="PXA33_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5119842.1"
                     /translation="MNNKRIKPEQFADFITQRTGRNLTCPICNSQEHYLHDGYDTFTE
                     LCGEKIIGVPTIPYRIEPSPDEVMKFAAPEFHSLHFNEREGTKYWLDQKARTAVIVTC
                     VCCNNMLFFDRERILEWIKGSQNE"
     gene            complement(847..1506)
                     /locus_tag="PXA33_000478"
     CDS             complement(847..1506)
                     /locus_tag="PXA33_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HDL5119843.1"
                     /translation="MTTLGSRIKAYREQLGISQYEVAERCTSIDNRDKNNKWGQSRIA
                     NYERNNRTPDLDDIEILSKVLGVSPETLAFDTNVQLARPTKSNSYPLISNIQAGLWTE
                     AFDFKDSEGYDYIDTEIDAGPDAFFLRISGMSMEPKFSEGDLVLIDIRKRPHPGDYVA
                     AVNGTGEATLKRYRELGEWSESGNPHFELIPLNPDFPTLSSMKQDIRIIGVAVEHRSY
                     L"
     gene            1636..1842
                     /locus_tag="PXA33_000479"
     CDS             1636..1842
                     /locus_tag="PXA33_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005656653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL5119844.1"
                     /translation="MNKIAEIRNQIGISQAQLAVQIGWGQPRIANYETGNRTPSIYVA
                     QKIVEGLNELGANVRIDDVFPVGN"
     gene            1863..2123
                     /locus_tag="PXA33_000480"
     CDS             1863..2123
                     /locus_tag="PXA33_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5119845.1"
                     /translation="MPRNELTKNARAIADLIHRKSATVTHKKLARAIGLSESQFSRTF
                     ADNVEMVAVIIDYLSIELADKDELLELKEKRYKRINLEEATT"
     gene            2287..2457
                     /locus_tag="PXA33_000481"
     CDS             2287..2457
                     /locus_tag="PXA33_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5119846.1"
                     /translation="MKNQRNDTQKSSLWQEFEYRKRLKNLIESGLSVAEIETRSQQIY
                     EELKSECTTEAN"
     gene            2432..3301
                     /locus_tag="PXA33_000482"
     CDS             2432..3301
                     /locus_tag="PXA33_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HDL5119847.1"
                     /translation="MNAQPKQINPILKLHPKQSEAKKVSVDDGYTQIPNELLKAILRS
                     GVLGWKGSYLLATVLKTLSWHKESDWFTHSQVCEMMNIEPTKYHINQLSAARKELIRE
                     NILFEDGKETGVNLSVFEWKMVYPEKVGSSRNNRELIPEKVGNGYPEKVGNTKETITK
                     EKINNEFTNVNSVQQPETSLPKKTKSEPVDYQGVMEEFNRAVEDTPIPQIRAMSDERK
                     RSVHALAKILRKEFGGYTRQHFADYFNDFVRQASSRKDRFYFGGLDGSAWVANFGYII
                     RSKTFYKTWEDSL"
     gene            3298..4659
                     /locus_tag="PXA33_000483"
     CDS             3298..4659
                     /locus_tag="PXA33_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
                     /protein_id="HDL5119848.1"
                     /translation="MSQNLKNITYEVEYLLVGALLKSGLNSKARDVLSWLEPEMFATF
                     QLGVIYEAIRKQALKDNVIDMLLLNTDYGQDFATLAEIMKNTISGANLDGYAEKVRQY
                     HQRREAQRVFLDVAGELQNARDEQLDAITSTGLAHLSKLLQRGGKVKPIDMNDLLEGY
                     LELFQERAKPSFKERLLFTGIEALDGKLGGINDTDICIVAGRAGNGKTETAITFTKNI
                     IENKGSVLFFSLEMSKEQIMDRLIASASGVNSVKLRNPEMMNDEDFARMGTAIQPLQN
                     QQLYIVDKSGLTAEEIVAIAESHIQEYGKVSAVVIDYIGLVRHGKLDGKINRTYQIGE
                     SMERFKTFCKNNHTPMILLAQLNRNADGSRPTNADLRDSGSLEQDASQIIMVHNQRNK
                     DGEPAPYTEWIVTKNRFGANGTVYMQFQQGRFVECDQAEAREYFQPKETKTSSNKRYG
                     AMQ"
     gene            4656..5192
                     /locus_tag="PXA33_000484"
     CDS             4656..5192
                     /locus_tag="PXA33_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HDL5119849.1"
                     /translation="MTDFDKNIKYKIIYADPPWKYKDSGCNGSAENHYTTMNIKEICD
                     LPVQDISDKDSILFLWVTYPMLPEGLKLIEAWGFNYKTIGFQWVKTNKKNKNSFFFGL
                     GRWTRGNTECCLIATKGKPSRVSNKVSQLIIEPIQHHSKKPDIVREKIVELMGDLPRI
                     ELFARNKTQGWDVWGDEV"
     gene            5283..5498
                     /locus_tag="PXA33_000485"
     CDS             5283..5498
                     /locus_tag="PXA33_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5119850.1"
                     /translation="MITYKCPKCNGELEDLSINDEWGWFLDEPYRCNGHYTGRFPNIS
                     RDSTLNRTKSCGYFSKEEVKKVNGTQR"
     gene            5482..5988
                     /locus_tag="PXA33_000486"
     CDS             5482..5988
                     /locus_tag="PXA33_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HDL5119851.1"
                     /translation="MAHSVNDIVKAHGRRYKARLKIQMIKMQGGVLVPLNEREAEALV
                     SLKNGEQYEIEVIRTRNPAFHRKVFAFLNFCFEYWSADKTEWEFFDERKQFDTFRKNL
                     TVLAGFKEITYTLDGRMRVEAQSLSYGNMEQDEFEQVYKALINAAIKHVFNNTKDENR
                     INQLYSFF"
     gene            6038..6250
                     /locus_tag="PXA33_000487"
     CDS             6038..6250
                     /locus_tag="PXA33_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5119852.1"
                     /translation="MKKNVIRYWLSVPFRIIAVLFFLVILTVRYVGYLLIYPLAFFAA
                     VGSFIVFCQWTYQEWLYYFKRGFAPI"
     gene            6296..6490
                     /locus_tag="PXA33_000488"
     CDS             6296..6490
                     /locus_tag="PXA33_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5119853.1"
                     /translation="MTKFFETIAFVMLFGIIPSCLLAINTTLMNMNIKNAPWFMVIIQ
                     LGFNITCYWLWFISVMRNVI"
     gene            6618..7187
                     /locus_tag="PXA33_000489"
     CDS             6618..7187
                     /locus_tag="PXA33_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HDL5119854.1"
                     /translation="MANKPPKQHKCKECGSYYIKFQSTQQVCSVKCAMAMGKRKTEAK
                     RKKSAKIDRLEQRKRLEKLKSRRDFEKDLQKIFNKFIRLRDKDLPCISCGRYHQGKYD
                     AGHYKTVGGNPELRFNEDNCHSQCVPCNRHLHGNIVNYRVNLIEKIGLERVEFLERKD
                     HPPLKLTIEQIKDLIKVYKAKCKELERVT"
     gene            7177..7650
                     /locus_tag="PXA33_000490"
     CDS             7177..7650
                     /locus_tag="PXA33_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5119855.1"
                     /translation="MLPRKIKNKWLEPEKQKWIEELLRLWGAWEFGGLDLESRVNMIY
                     RLMKSAEGVKSLSSREVCNDSLGTLINEIFTVVTKRDVMLADILKQKYWFGRSERQIA
                     IYYQLRDSEGRKERRWQEIIQDKCKKAERIIADILESKISSSNSTDKLKKYQFSP"
     gene            7878..7953
                     /locus_tag="PXA33_000491"
     tRNA            7878..7953
                     /locus_tag="PXA33_000491"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7911..7913,aa:Trp,seq:cca)
     gene            7970..8155
                     /locus_tag="PXA33_000492"
     CDS             7970..8155
                     /locus_tag="PXA33_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5119856.1"
                     /translation="MYNLDDAIQECREKFGEDKPYFSEAGTLTFAGCKALSRLRDEKL
                     GIKAPPNFPFPRVEDLS"
     gene            8181..8351
                     /locus_tag="PXA33_000493"
     CDS             8181..8351
                     /locus_tag="PXA33_000493"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5119857.1"
                     /translation="MTEQLNTQSKSGEEDQEKSQDRYAVGWPSRRISDCVQHFRSRKI
                     YPFASNNDVKQG"
     gene            8494..8739
                     /locus_tag="PXA33_000494"
     CDS             8494..8739
                     /locus_tag="PXA33_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5119858.1"
                     /translation="MKLMKDMGTQSYIWSGFSTWLAWLGEQQNLMLLSLAIGIVTAIA
                     GLIQRFDEWRRRKREDERAEELHQAKMARLRKGLSDE"
     gene            8732..9301
                     /locus_tag="PXA33_000495"
     CDS             8732..9301
                     /locus_tag="PXA33_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HDL5119859.1"
                     /translation="MSKSLKYGSGIVCGIAAIITLVQYQHPEIRTNQAGLEIIGNAEG
                     CRRDPYKCPADVITVGIGSTEFGGEKIDPNRIYSDKEIAERWAKDLKIAESCVNRHFN
                     GKDMNDNQFSGMTSAVFNMGCYNMRFYRNKQGQYVQTTIHKLAVNKQFEEMCHRLPDF
                     IRASGKVLNGLVIRREKEKALCLTGLVAR"
     gene            9274..9624
                     /locus_tag="PXA33_000496"
     CDS             9274..9624
                     /locus_tag="PXA33_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2570 domain-containing protein"
                     /protein_id="HDL5119860.1"
                     /translation="MLNWISGTIAIVILGLCVWLWGQSQMIDSLRAENRAQAQTIEQQ
                     QKANQRLTDSLEQERQAVEKIQKIANELRNKVEMAKNEITSILAQDSCAKADLPNGVA
                     DSIKRLHQQNNHKH"
     gene            9629..9802
                     /locus_tag="PXA33_000497"
     CDS             9629..9802
                     /locus_tag="PXA33_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5119861.1"
                     /translation="MYPPAAYLVPCGRTEFSGRTYGDTVEYLVKVMGERDLCANQIDR
                     IREWQAQTKQGFK"
     gene            10194..10718
                     /locus_tag="PXA33_000498"
     CDS             10194..10718
                     /locus_tag="PXA33_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HDL5119862.1"
                     /translation="MTKKGEVKATSKGVGKLTDKQKRFVEEYLIDLNATQAAIRAGYS
                     EKTAYSIGEENLRKPEIRSAIQEAQNKRSERTQITQDDVLNGLLEVIAMSTGKKIVTE
                     TDVAKNDNGELVGFDIAKTKFEPAAANKALELLGKHLGMFKDKVDLINSDGSLNRPMV
                     IELIAPSLDDESTD"
     gene            10702..11922
                     /locus_tag="PXA33_000499"
     CDS             10702..11922
                     /locus_tag="PXA33_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HDL5119863.1"
                     /translation="MKAQIELPPKLLPVFAKDYRYKGAYGGRGSGKTRAFAKMSAVSA
                     YKRAMRGESGVILCGREFMNSLEESSLEEVKQAIKSEPFLANFFDVGEKYVRTKDGRI
                     SYVFSGLRHNLDSIKSKARILIAWIDEAETVSETAWRKLIPTVRENNSEIWVTWNPEN
                     RGSATDTRFRQNPPENSCIIEMSYQDNPWFPDVLEQERLNDKQRLDDATYRWIWEGAY
                     LEASEAQIFRGKYQEMEFVPNPDFDGPYYGLDFGFAQDPTAAVKCWVFNGDLYIEFEA
                     GKVGLELDDTADFIAKGVPQIADHVVRADSARPESISYLKRHGLPRIIGVEKWKGSVE
                     DGIEHIKSYGKVFIHPRCQQTLNEFRLYSYKTDRLSGDVLPVVIDAHNHFIDALRYAL
                     TPLMQVKSAKGVLL"
     gene            11919..13295
                     /locus_tag="PXA33_000500"
     CDS             11919..13295
                     /locus_tag="PXA33_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HDL5119864.1"
                     /translation="MSLEQDRLAFLAEALGLGNIKRRTLWKEFGYPNTLTFNHFLKAY
                     KRNSIAFAAINRLLDGCWVDCPVIVEGEQKNESKQTTEWESKVERFMKRYWSAIKEAD
                     RRNLVGNYSALLLQVRDGQQWDQPILQGALSSIGEFGLVKLIPVWQSQLSVTEFQEDV
                     TADNYGEPLMYQFSESAFGKKSVSRNIKVHASRVILLNEGGDFNSPHSGVPLLEAGYN
                     KLVDLEKTSGGSAEGFLKNASRQLGIKLTKDVDLRQLEDSAKALGFSSFSDALNDKIK
                     KINSGTDSALITHEGDASVLSVAPADPKPTWEISANEFAASVQIPFTILFGQQTGRLA
                     SDEDKTDWANRCNGRRNGFLTDVITQLLNRLWFIGVLPMPKNSDVTVSWSDLLAPSEK
                     EKIANAQALASVATTSQSAFGFAAVTANEIRESLGFEPLPDNLIPPTIDDENEDQDEN
                     QATADSDK"
     gene            13249..14187
                     /locus_tag="PXA33_000501"
     CDS             13249..14187
                     /locus_tag="PXA33_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017357793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HDL5119865.1"
                     /translation="MKIKTKIKPLRIPTNKADPLNMGVSVAKLFRHIDVIYSQIKRSI
                     KQQILQKMSLRLQANNFEYFPSYLTATELADLLETLQKIIDKEILSNGSQGENLWFDS
                     YIDEAALKGTQSAVTDLGLQSEAYRSQRNLSSIVFSPAYFNRLAIAHMASYSEWQGLS
                     DELRKSLAGVITEAVLNGKNVKETAREIRQKLDVSSKRAKRIAQSSQLAAYRRAEWQE
                     AEEAKEELGLNTKLLHFSALKATTRLAHAQRHGKYFDVAEVREWYSRDGNQFNCYCKQ
                     SVVVVNADGKTDIEPLLVGLAKERKQWVGALKNVKK"
     gene            14174..15544
                     /locus_tag="PXA33_000502"
     CDS             14174..15544
                     /locus_tag="PXA33_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016533290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HDL5119866.1"
                     /translation="MSKNNVNIVTVINSKNISTESIDGDQHLIIRGVVPIVDDVVMND
                     GLYPADEINKSYNSLEGNFMPLGHPKIEGKYVSAQDVRAVNKHHVGAWAKNVRKESGK
                     VLVDMYINRRFAEGSEQGKTLLERLDGMVTNTNAEPIHVSTGLILNKVKQSGKSKGKK
                     YSWIATNMSFDHIAILLDVPGAATPDDGVGIFVNQDGSEMGVENVNLTQAADYRTEKL
                     FDKVRFYLMANSDLSFEEIHRALREAIRPEGKDKWSFWVEAVYPKYFIYSDDNSGKKY
                     KRSYFIDENDNVQLTSEPVEVVKKIGYEEITTNEEHNPMKTKILAVLNAANVKTEGLN
                     DDQLLEAYNKLQADKGKEPEKPKNEDDSEIDEKIKKAVNAAIEPLQQALQANTDKEQA
                     AMRAAVKAKFNMSDAAVNALQGEVLTELYAQTQQSVGLNNNSANGNGDNQWDGYSLNQ
                     EEKGNE"
     gene            15537..15971
                     /locus_tag="PXA33_000503"
     CDS             15537..15971
                     /locus_tag="PXA33_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5119867.1"
                     /translation="MSNVIYRGSVKREPQTVNIVINDTSAPGAVVKLHNGKLEAAADL
                     KGRRFLLGNNRFKGQTIEQAYAKGDTATAFRLEPEQEYYAQIADGTYNFGDELTVKQA
                     GGKLTKAETGDVVLFFFDEESQRVISGGKGYADVVVANAYTK"
     gene            15986..16975
                     /locus_tag="PXA33_000504"
     CDS             15986..16975
                     /locus_tag="PXA33_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="encapsulin"
                     /protein_id="HDL5119868.1"
                     /translation="MLKFTKEQERFVINERINYDKKHALMAANSAGLLGNAMTLPKDV
                     WAEWDRDAVQIQREELVVFNDLAGISKSMPIGKLIHHFQTVSDSGSVNISLDGRSSAK
                     TDNVVLNYHGTPLPIIDSTFSFGWRDMSAAQSEGYQIDGAARANSLRKVAEKMEDLAL
                     NGDSSIVVGNAKLYGLRTAPNRMTNTHNLDLATATPKEIYDVFRDLISKFHAKNYYSP
                     VTLYVNYGDYFAMSTRDYSEQKSEGSILNKVMTIPQIAKIVPASRVPQNEILGLCKRS
                     DVYQVLNGMPLVTRPIARHNETDDYSFQIMAAVAVEFKFDDKGNAGYIQYTKS"
     gene            16987..17238
                     /locus_tag="PXA33_000505"
     CDS             16987..17238
                     /locus_tag="PXA33_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5119869.1"
                     /translation="MKWLLTHDSHELKKGDVYEGETLPLWLVGKAIEIEEKSFEVATP
                     SDHADGETGETGETGETGETGETGETGETGQKGKKDKGK"
     gene            17241..17618
                     /locus_tag="PXA33_000506"
     CDS             17241..17618
                     /locus_tag="PXA33_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5119870.1"
                     /translation="MAAEIELSEARTTLDELGFTSPNSLLELYVQQTNTLDQALNKSG
                     YSEETQKLIKLYLVAILAISSGARRIKSEGAPSGASRTFEYDPNALDNLKSAIRSIDP
                     KGCAESLLPKDKVVGFFDVVGGC"
     gene            17618..17962
                     /locus_tag="PXA33_000507"
     CDS             17618..17962
                     /locus_tag="PXA33_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5119871.1"
                     /translation="MSNLSNWSYTAKATLWQAKGKNDDGVLVFSTPQIISCDYGADRK
                     RARFEIGREQAVKNVIWTEFAHAKLGDYVLIGESEQADPILAGAEEIIHIQRFADTFD
                     RNRDDFALITGG"
     gene            17964..18332
                     /locus_tag="PXA33_000508"
     CDS             17964..18332
                     /locus_tag="PXA33_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HDL5119872.1"
                     /translation="MGIKVKGIRQVKQRLNQVVGEVQSQKATRAMFRILNAVAPLAAH
                     YTPVDTSTLINSQFTEMEVNGTRLIGRVGYSANYAAYVHDPNIKQNFRKPSAKKEFLT
                     SALNESKATIKSIVKEELSL"
     gene            18329..18709
                     /locus_tag="PXA33_000509"
     CDS             18329..18709
                     /locus_tag="PXA33_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5119873.1"
                     /translation="MISYVKAFKQWLEAHSLADGYVVQLYQWEDTQKAKPVIVIQPNS
                     GSPQVSDLSSEHYLLISLVAGKNSGYTIEERARAIMSKILVEPFASFGYIESMGGLPA
                     PIFTEDNRMIFRLPLRIISTNQEE"
     gene            18713..19195
                     /locus_tag="PXA33_000510"
     CDS             18713..19195
                     /locus_tag="PXA33_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL5119874.1"
                     /translation="MGSPSPQNNAENLVVGRAVVLEYGTTDAKPQESEWKVAGAMTTK
                     SWDFSPNSVTSEADDAGGFPETLITNSDFSISGDGEWRKRPKSKELGIKDLVVLYANA
                     VKNRTQPYIWVRLKYAGLTILGKMVITALSSEAPTNDLVKFSIEFKVGDSSTIEIAAA
                     "
     gene            complement(19235..19651)
                     /locus_tag="PXA33_000511"
     CDS             complement(19235..19651)
                     /locus_tag="PXA33_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HDL5119875.1"
                     /translation="MFYPALFTPAEEGGFVVTFPDLPEAITQGDTFEEAMEMAEDVLL
                     SCVEIYFDEDRRFPLTRPVNTDEVPVFMPESIYAKVLLHNTMQEQAVSKAEIARLTNI
                     RPPEVQRILAPRHITKIDTIGRILASLGKPLQLSLG"
     gene            complement(19707..19883)
                     /locus_tag="PXA33_000512"
     CDS             complement(19707..19883)
                     /locus_tag="PXA33_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010848235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HDL5119876.1"
                     /translation="MKQSEFLRWLKANGVEVENGSKHLKLYYKGKRSHLPKHPSQELK
                     TGLVEGVKKQLGLK"
     gene            19979..20650
                     /locus_tag="PXA33_000513"
     CDS             19979..20650
                     /locus_tag="PXA33_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5119877.1"
                     /translation="MKMVSYPITEIGEMLIATDGRDFLFRPSFKNIYKIGSPQEIVKI
                     YALLHGCEIREILPHILTKSESHQSYFWSVLNSPVFGRKILQNAMIVMSCCCDDDISL
                     LIGEWLPSKSGVRYKLGKMPPETIINFAKNLMGHGVIGCCELPKKEQSAKNEFSESFD
                     AVEYISLARTHFNMSREDAENLTMTELQQLLKSQMPAENNSYGQFTEEEYDRIMAEYE
                     KRRAA"
     gene            complement(20665..20898)
                     /locus_tag="PXA33_000514"
     CDS             complement(20665..20898)
                     /locus_tag="PXA33_000514"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5119878.1"
                     /translation="MKKDCTKQDFANWLNENQEVCWIKEVSIERILSCVDDYVAICNA
                     RSKDEFELLNDIRELFYSAHYKDFTPVLSGRFF"
     gene            21067..>21479
                     /locus_tag="PXA33_000515"
     CDS             21067..>21479
                     /locus_tag="PXA33_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5119879.1"
                     /translation="MKKMIVFVASVLSSFVIASDWEIIEAAQNKVKAELIRKSEKKAE
                     LCLNVAQAVLITNQKVQKDTFSMCGEFSTEIVFSDSKAYSLAVCGKVKGKDIIGNPIE
                     SDYIYQRATKDFYFNYNGKKSAYYDPQSDLARLQEV"
BASE COUNT         6673 a         4034 c         4982 g         5790 t
ORIGIN      
        1 tcgctgctca acattgtcta gtcgttgttc gacattatcc aatctagatt caactttagt
       61 gaggcgaata tcaagatttt tgatattggt ctctacttgg acaaacttat catcaatttt
      121 cgagaatcga gcttcaactt tggtctcaag gtgtgaatat actgtccata atgcaattac
      181 agcagtaact actaatgcaa tgagtgtaga gccaccagct ttataaaaag cctctttcgt
      241 caaataattt gacttgattt ctcttacatc gccttcaact tgtcttaatc gagcatataa
      301 attcacttct tccgtctcct gataactaat ttgtgaatta ttatgcccgc cgtatactgc
      361 atttgcaagt aaggatagta tgattgcatc tctaggatca ttttgaatca ttggtctatt
      421 cattttgtga gcccttaatc cattctaaaa ttctctctct gtcgaaaaaa agcatattat
      481 tacagcaaac acaagtgaca ataacggctg ttctcgcttt ttggtctaac caatattttg
      541 ttccttcccg ttcattgaaa tgcaaagaat gaaattcagg agctgcaaat ttcataactt
      601 catcaggaga tggctctatt cggtatggaa tggttggaac accgataatt ttctctccgc
      661 ataattccgt aaaagtgtca tagccatcgt gtaaataatg ttcttggcta ttacaaatag
      721 ggcaagttaa attccgccca gtacgttgcg tgataaaatc cgcaaactgt tcaggtttaa
      781 tgcgtttatt attcatttta acttcctttc tacccaataa aattcaactg ttagctttgg
      841 tggactttac aaatagcttc tatgctcaac agcgacacca ataatacgaa tatcttgttt
      901 cattgagctg agtgttggaa agtcggggtt aagcggtatt agctcaaaat gaggattgcc
      961 ggattcagac cattcgccta actcccgata acgttttaaa gtagcctcac ctgtgccatt
     1021 aaccgctgct acataatcgc ccgggtgagg acgtttgcga atatcgatta acaccagatc
     1081 gccttcacta aatttaggct ccatcgacat tccggaaatt cttaaaaaga aagcgtccgg
     1141 tccggcatca atttccgtat cgatatagtc gtagccctcc gaatctttga aatcaaaggc
     1201 ttctgtccat aaacccgctt gtatattgct gatcaacgga tatgagttcg attttgtcgg
     1261 tctggcaagc tgaacgtttg tatcaaaagc aagtgtttct ggagaaacac caagcacttt
     1321 gctgagtatt tcaatatcat caagatcggg agtacgattg tttctctcat aattagcaat
     1381 gcgagattgc ccccatttgt tatttttatc tcgattatct attgatgtac atctttcagc
     1441 aacttcatat tggctgattc ctagctgttc tctgtaagcc ttaattctac tacctaaggt
     1501 tgtcatagca ctctcctatt agattttcct cattttaaca cgctccgtta tatccttgtg
     1561 aatcacaaaa tgaaatttac aattagttca ttttgtgatt taattaaaag atataaatca
     1621 caaagagaaa tttgtatgaa taagatagct gaaatacgaa accaaattgg tataagtcaa
     1681 gctcagttag ctgttcagat tggttggggg caaccaagaa tagctaacta tgaaactgga
     1741 aatcgtaccc catctattta cgttgctcaa aaaattgttg agggattgaa cgaattaggt
     1801 gcaaacgtac gaattgatga cgttttcccc gttggcaact aatttacccc aagaggcttg
     1861 caatgccacg caatgaatta acaaaaaatg caagagcgat tgcggatttg attcatcgaa
     1921 aatccgcaac ggtgacgcat aagaaactag caagagcaat cggactgagt gaatcacaat
     1981 ttagccgtac atttgcagat aatgtggaaa tggtggcggt gattattgat tacttaagta
     2041 ttgagcttgc ggataaagac gagttactag agctgaaaga gaaacgatat aagcgaataa
     2101 atctcgaaga agcgacaaca tagaagttac caacaatgaa aaatttttta gaaaaattca
     2161 aatcattcta ccacagtaaa aattcaattt ttattagagt ggcaattttt atgctgcttg
     2221 cgtggtgggt ttttaacaat taaaaaacca ccgctggaac ggtggtaatt tacaaggagt
     2281 taccttttga aaaatcaacg caatgataca caaaaaagct ccttatggca agagtttgaa
     2341 tatcgaaaac ggttaaaaaa tctcattgaa agcggtttat ccgtagccga aattgaaacc
     2401 cgttcacaac aaatttatga ggagctgaaa agtgaatgca caaccgaagc aaattaaccc
     2461 aattttaaaa ttgcacccta aacaatcaga ggctaagaaa gtgagtgttg atgatggtta
     2521 tacacaaatt ccgaatgagt tattaaaagc aattttgcga tcgggtgttt taggttggaa
     2581 aggctcttac ttactggcta ccgtcttaaa aacattgtct tggcataaag aaagtgattg
     2641 gttcacgcat tcccaagtgt gtgaaatgat gaatattgag ccgacaaaat accatattaa
     2701 tcaactttcc gctgctcgaa aagaacttat ccgtgaaaat atcttgtttg aagatggtaa
     2761 ggaaacaggg gtaaatctca gtgtttttga atggaaaatg gtatatcccg aaaaagtagg
     2821 gagttcccga aataataggg aattaattcc cgaaaaagta gggaatgggt atcccgaaaa
     2881 agtagggaac acaaaagaaa ctattacaaa agaaaaaata aataatgaat ttactaacgt
     2941 aaattctgtg caacagcccg agacctcttt gccgaaaaaa acaaaatctg agccggtcga
     3001 ttatcaagga gtgatggaag aatttaatcg tgctgtggaa gacacaccga ttccgcaaat
     3061 tcgggcgatg agtgatgagc gaaagcgatc ggttcacgca ttagcaaaaa tcttgcgaaa
     3121 agaatttggt ggctacacca gacaacactt tgccgattac ttcaatgatt ttgtccgaca
     3181 ggcaagtagc cggaaagaca gattttattt tggcgggctt gatggctcgg cttgggtagc
     3241 aaatttcggc tacatcattc gttcgaaaac gttttataag acttgggaag attcgctatg
     3301 agccaaaatt tgaaaaatat cacttacgag gttgaatatt tgctggttgg agcattgctg
     3361 aaatccggtc ttaactcgaa agctagggac gtattgagct ggttagagcc tgaaatgttc
     3421 gctacctttc aacttggggt gatttatgag gctatccgca agcaggcgtt gaaagataac
     3481 gtgatcgata tgctgttact caataccgac tacggacaag attttgccac gttggcagag
     3541 ataatgaaaa acacgatcag cggtgcaaac ttagacggct atgcagaaaa agtccggcaa
     3601 tatcatcaac gccgagaagc ccagcgagtg tttttagacg ttgcaggtga gttgcaaaat
     3661 gcccgagacg agcagttaga tgctatcact tcaaccgggt tagctcacct aagcaaatta
     3721 ttacaacgtg gcggaaaagt gaaaccgatt gatatgaatg atttgctcga aggttatttg
     3781 gaattgttcc aagaacgtgc aaaaccaagt tttaaagagc gtttgttgtt taccggtata
     3841 gaagcattag atgggaaact aggcggtatt aacgataccg atatttgcat tgtagcagga
     3901 cgtgccggta acggtaaaac cgaaacagcc atcacgttta ctaaaaacat tatcgagaat
     3961 aaaggttcgg tgctgttctt ctcgctggag atgagcaaag agcagattat ggataggctg
     4021 attgccagtg caagcggtgt aaattcagta aaacttcgca atccggaaat gatgaatgat
     4081 gaagattttg cccgaatggg aacggcgatt cagcctttac aaaatcagca gttgtacatt
     4141 gttgataaaa gcggattaac cgcagaagag attgtggcga ttgcagagag ccatattcaa
     4201 gagtacggca aagtgagtgc ggtagtgatt gattatatcg gtttggttcg acacggcaaa
     4261 ctagacggca aaattaaccg aacctatcaa atcggtgaaa gtatggagcg gtttaaaact
     4321 ttctgcaaaa acaatcacac accgatgatt ttattggcac agcttaaccg caatgcagat
     4381 ggcagtcgcc caaccaacgc agatttgcga gacagtggca gtcttgaaca agatgcaagc
     4441 caaatcatta tggtgcataa ccaacgcaac aaagacggcg agccagcccc ctatacagaa
     4501 tggattgtta ctaaaaatcg ctttggagca aatggcacgg tttatatgca attccagcag
     4561 ggtaggttcg tagaatgcga ccaggcagaa gcgcgggagt atttccagcc gaaagaaact
     4621 aaaacatcat caaacaaacg ctatggagca atgcaatgac agattttgat aaaaatataa
     4681 aatacaaaat tatttacgca gatccgcctt ggaagtataa ggatagtggc tgtaatggga
     4741 gtgcagaaaa ccattacaca acaatgaata tcaaagaaat atgtgattta cccgtccaag
     4801 atatttcaga caaagattct attctgtttt tgtgggttac ttaccctatg ttacctgagg
     4861 gcttaaagtt gattgaggcg tgggggttca actataaaac aatagggttt caatgggtga
     4921 agacaaataa gaagaataag aattcttttt tctttggctt gggacgatgg actagaggta
     4981 ataccgaatg ctgtttaatt gcaacaaaag gaaagccatc gagggtaagc aataaagtga
     5041 gccaattaat tattgagcca atacaacatc atagtaaaaa gcctgacatt gtcagagaaa
     5101 agatagttga gttgatgggt gacttgccaa gaatagagtt gttcgccaga aataaaaccc
     5161 aaggttggga tgtttggggg gatgaggtgt gattgtttca agctattgtt acccaaagtg
     5221 cggtagggtt ttattaaaaa ggttggcggt tgtgaaggat aacgaaggtt gggacggttt
     5281 agatgattac ttataaatgc cccaaatgca acggagagct tgaagattta agtattaatg
     5341 atgaatgggg ttggttttta gatgagccgt atcgttgcaa cggacactat acagggcggt
     5401 ttccaaacat tagccgagat agcacgttaa atcgcacaaa atcctgtggg tactttagca
     5461 aggaagaggt taagaaagta aatggcacac agcgttaatg acatcgtaaa agcccacggc
     5521 aggcgttata aagcaagatt gaaaatacag atgataaaaa tgcaaggagg cgttttagtc
     5581 ccccttaatg agcgtgaagc tgaagcattg gtatcactta aaaatggcga gcaatatgaa
     5641 attgaggtta ttcgcactcg caatccagcc tttcatcgga aggtcttcgc ctttttaaat
     5701 ttttgctttg aatattggtc ggcagacaaa accgaatggg agttttttga tgagaggaaa
     5761 cagtttgata ctttccgaaa aaatctgact gttctggcag gatttaaaga aatcacatac
     5821 accctagatg ggcgtatgcg tgtagaagct caaagtctta gttacggaaa tatggaacaa
     5881 gacgagttcg agcaggttta caaagcattg attaatgcag ctatcaagca cgttttcaac
     5941 aataccaaag atgagaatag gataaatcag ttgtattcgt ttttttaatt aaggcgaaat
     6001 cgccatattt aattaggttt aattgcagga gattaaaatg aaaaagaatg ttattagata
     6061 ttggcttagc gtgccattca gaattattgc agtgcttttt ttcttagtca ttcttactgt
     6121 tagatatgta ggttatttat tgatttatcc attagcattc tttgcagcag tcggtagttt
     6181 tattgttttc tgtcaatgga catatcaaga atggctttat tattttaagc gcggttttgc
     6241 tccgatttag tgtgtgttta ttaattccct cgaattcggg ggaaggagaa gtaagatgac
     6301 aaaatttttt gaaactatcg cattcgtgat gttatttggc attattccaa gctgtttgtt
     6361 ggcgattaat actactttaa tgaatatgaa tattaaaaac gcaccgtggt ttatggtgat
     6421 tattcagtta ggttttaata tcacttgtta ttggttgtgg tttatttctg ttatgcggaa
     6481 tgttatttaa ggagattaat aagtgagcca gtgcaatgaa ctgactgtaa ttttaatcgc
     6541 catcgctatt cttagtgtat gtgccggttc actctttttt attgagtggt ttgataagag
     6601 agatggtgag ggttggaatg gctaacaaac cacctaaaca acacaaatgc aaagagtgcg
     6661 gcagttatta catcaaattt caaagcacgc agcaggtctg ctctgttaag tgtgctatgg
     6721 caatgggtaa gcgtaagaca gaggcgaaac gcaaaaaaag tgcaaaaatc gaccgcttgg
     6781 agcaacggaa gcggctggaa aaattgaaaa gccgtagaga ttttgagaaa gatttacaaa
     6841 agatatttaa taaattcatt cgcttgcgtg ataaagattt accctgtatt tcctgtggcc
     6901 gctatcatca ggggaaatat gatgcagggc attataaaac cgttggtggt aatcctgagc
     6961 tacgttttaa tgaagataat tgccacagtc aatgtgtgcc gtgtaaccga catctgcacg
     7021 gcaatattgt gaattatcga gtaaatctga ttgagaaaat cgggcttgag cgtgtggaat
     7081 ttttagagcg taaagaccac ccaccattaa aacttactat cgaacaaatc aaagatttaa
     7141 tcaaagtcta taaagcgaag tgcaaggagc ttgagcgtgt tacctagaaa aattaaaaac
     7201 aagtggttag agcctgaaaa gcagaaatgg atcgaagagt tgctaaggtt atggggggcg
     7261 tgggaatttg gcggattgga tttagaaagt cgggtaaata tgatttaccg gctgatgaaa
     7321 tctgcggagg gggttaaaag tctttcatct agagaggttt gtaacgatag cctcggcacg
     7381 ctgatcaatg agatttttac ggtagtcacc aaaagagacg taatgttagc cgatatactc
     7441 aaacaaaaat actggttcgg gcgttcggag cggcaaatcg ctatttatta tcaactaagg
     7501 gacagcgagg gtagaaaaga aagacgatgg caagaaatta ttcaagataa atgcaaaaaa
     7561 gcggaaagaa ttattgcaga tattcttgaa agcaagattt catccagtaa ttctacagat
     7621 aaactgaaaa aatatcaatt ttccccttga ttttagtgcg taacaagtgt atattttgtg
     7681 ctaatggtgg acgtagtata aatgatgttt accaatgtta gttaaagacg cagcctgaag
     7741 taatattcag ttcggaggag aataactaga aacgacgtcg agtatagtta tttcagcgtt
     7801 tgcgtggcag tatgccgacc gaattagttt aacagccctg agcagaaatg cttggggctt
     7861 ttttgtttac ggattttaag ggtgtagctc agttggtaga gcagcggtct ccaaaatcgt
     7921 tggttgttgg tttgagtcca accacccttt ccaaatgcga gtaaatgtta tgtataacct
     7981 tgatgatgca attcaagaat gcagagaaaa atttggtgaa gataagcctt atttttctga
     8041 agctggaaca cttacttttg ctggttgcaa agccctctct cgattaagag atgaaaagtt
     8101 aggaattaag gctccgccaa atttcccttt cccaagggtt gaagatctat cttagctaca
     8161 aaaatcaatg agggattgct atgacggaac aactaaatac tcagtcaaaa agcggtgaag
     8221 aagatcaaga aaaatctcaa gatcgctatg ccgtgggctg gccatctcga cgtatatcag
     8281 attgcgttca gcacttccga agtaggaaaa tttatccatt tgccagtaat aatgacgtga
     8341 agcaaggtta aatgcttctg gtggtacaga gtaaattcaa tcatttttac cctctgctag
     8401 tttatttgtt ggggaacagt attctagcag aatttttaac caaagctcag tctcacgact
     8461 gggctttttt attgcctcga aacggggtgg agtatgaagt taatgaaaga tatgggaacg
     8521 cagagctata tttggtcggg atttagcact tggctggcgt ggctcggcga acagcagaat
     8581 ttaatgttac ttagtcttgc tatcggtatt gttactgcca ttgcagggct tattcagcga
     8641 tttgatgaat ggcgtaggcg taagcgtgaa gatgagcgag cggaagagct tcatcaagcg
     8701 aaaatggcaa gattgagaaa ggggttaagt gatgagtaaa agccttaaat atggcagtgg
     8761 tatcgtttgt ggtatcgctg ccattattac attagtccaa tatcaacatc ccgaaatccg
     8821 cactaaccaa gctggattag agattatcgg taatgctgag ggttgcagac gtgatccgta
     8881 taaatgccct gccgatgtaa tcacggttgg cattggctca acggagtttg gcggtgaaaa
     8941 gattgaccca aatcgtattt attctgacaa agaaattgct gagcgttggg caaaagattt
     9001 gaagattgcg gagagttgtg tcaatcgtca ttttaatggt aaagacatga acgataatca
     9061 attttcggga atgacgtccg ctgtatttaa tatgggttgt tataacatga gattttatcg
     9121 gaataaacaa ggtcaatatg tgcagactac catccataaa ctcgcagtga ataaacaatt
     9181 tgaggagatg tgtcatcgac tacctgactt tattcgtgcc agtggtaagg tattgaacgg
     9241 attggttatc cgccgtgaaa aggagaaagc attatgctta actggattag tggcacgata
     9301 gccattgtga ttttgggctt gtgtgtatgg ttatggggtc agtcacagat gatagatagc
     9361 ttaagagctg agaaccgcgc acaagcccaa accattgagc agcagcaaaa agccaatcaa
     9421 cggctaaccg atagcttaga gcaagagcga caagcggtcg aaaaaatcca aaaaattgca
     9481 aatgagctgc ggaataaagt ggagatggcc aagaatgaaa ttacatcaat actggcacaa
     9541 gacagttgtg ctaaagctga tttgcctaat ggcgttgctg atagcattaa gcggctgcac
     9601 cagcagaaca atcacaagca ctgagtattt gtatccaccg gcagcttacc ttgtgccgtg
     9661 tgggcgaaca gaatttagcg gtagaaccta tggcgatacg gttgagtatc tcgtcaaagt
     9721 gatgggagag cgtgatttgt gtgctaatca aattgaccgt atcagagaat ggcaagcaca
     9781 aaccaaacaa gggtttaaat agctaattat ctaattaacc gctattgaaa atgggagcgt
     9841 aacagctccc tttttgtttt gatggaaagt aaatattaca taatttagct atggtttcgg
     9901 ggttggttgg ataaaacacc gaggaaatac ctaagtgatg tgatttatat aaaacaaaac
     9961 cccgaacatt tgcggtgttc gaggtttttt tattacccat tgaaacggaa tgagtaacaa
    10021 gttatggaaa ttattacatt tttaaccgta accatcaagg agattcttat ggaatatggt
    10081 ttatggcaaa taagcctagc agtaacagtc cctattttgg cgttcgtgtc gccaaagctg
    10141 attaatgcta ttgctaatct attaaatgca atgaagtaaa ggaatttaac ccaatgacta
    10201 aaaaaggcga ggttaaagcc acgtctaaag gcgtgggtaa attaaccgat aaacagaaac
    10261 gatttgtaga agagtatctg attgacttga atgcgactca agctgcgatt agagctgggt
    10321 acagcgaaaa aacagcatac tcaatcggag aagagaacct gagaaaacct gaaatcagaa
    10381 gtgcaattca ggaggctcaa aataaacgct cggagagaac ccaaattaca caagatgatg
    10441 tgctaaatgg gttgcttgaa gtgattgcaa tgagtactgg taaaaaaatt gtgacagaaa
    10501 ctgatgttgc aaaaaatgac aatggtgagt tggtcggttt tgatattgca aaaacaaaat
    10561 tcgaacctgc cgccgcaaat aaagctcttg agttactcgg taaacattta ggaatgttta
    10621 aagacaaggt tgatttaatt aattctgatg gttcactgaa tcgaccgatg gtgattgaat
    10681 taattgctcc gagtttagac gatgaaagca cagattgaac taccacctaa actgttgcct
    10741 gtttttgcca aagattatcg ttacaaaggt gcttatggtg ggcgaggttc aggcaagact
    10801 cgggcatttg ccaaaatgtc ggcagtgtct gcttataaaa gagcaatgcg aggggaaagt
    10861 ggcgttattc tttgcggtcg tgagtttatg aactcattgg aagaaagctc tcttgaagaa
    10921 gttaaacagg cgatcaagtc tgagcctttt ttagcaaatt tttttgatgt cggcgagaag
    10981 tatgttcgca ctaaagatgg gcggatttct tatgtgtttt ccggacttcg gcataactta
    11041 gatagcatca aatctaaagc ccgtattttg attgcgtgga tagatgaagc agaaacagta
    11101 agcgaaactg cttggcggaa attaattcca accgtgcgtg agaataattc cgaaatatgg
    11161 gtaacttgga acccggaaaa tagagggagc gcaacagata ctcgcttcag gcaaaatcca
    11221 cctgaaaaca gctgcataat tgaaatgagt taccaagata atccttggtt tcctgatgtg
    11281 ctcgagcaag agcggctaaa tgacaaacag cggcttgatg atgcgactta tcgctggatt
    11341 tgggaaggtg cttatttgga agcgagcgaa gcgcaaatct ttcgcggtaa atatcaagaa
    11401 atggaatttg tgccgaaccc tgattttgat ggcccatatt acggcttaga ttttggtttc
    11461 gcgcaagatc cgacggcggc ggtcaaatgc tgggtattta atggcgatct gtacatcgag
    11521 tttgaagcag gcaaagtggg cttagaactt gacgacaccg cagactttat tgccaaaggc
    11581 gtgccacaaa ttgccgatca cgttgtgcgt gcagattcgg cgcgtcctga gtcaattagt
    11641 tacctcaaac gtcacggctt accgcgcatt atcggtgtgg agaagtggaa aggctcggtg
    11701 gaagacggca ttgagcatat taagtcctac ggcaaagtct ttattcaccc acgctgtcag
    11761 caaacgctca acgaatttcg cctttattcg tacaaaaccg accgcttatc gggcgatgta
    11821 ttgcctgtcg tgattgatgc ccataaccac tttattgatg cgctacgcta cgcattaacg
    11881 ccgttgatgc aagttaaaag tgccaaagga gttttactat gagcctagag caagacagac
    11941 tggctttttt agccgaagcc ctagggcttg gcaatatcaa acgaagaacg ttatggaagg
    12001 agtttggcta ccctaatacc ttaacattca accatttctt aaaagcctat aagcgcaata
    12061 gtatcgcttt tgcagcgatt aaccgcttac ttgacggttg ttgggttgac tgccccgtaa
    12121 tcgttgaggg tgagcaaaag aacgaatcaa agcagacaac cgaatgggag tccaaagttg
    12181 aacgttttat gaaacgctac tggtcggcaa tcaaagaggc agatagacga aaccttgtcg
    12241 gtaactattc ggcgttatta cttcaagtaa gagatggtca acaatgggat cagcctattt
    12301 tgcaaggcgc gctatctagc attggagagt tcggactggt taaactgatc cccgtgtggc
    12361 agtcacagct ttcagtgaca gagtttcaag aggatgtgac cgccgataat tacggcgaac
    12421 cgctgatgta tcaattcagc gaatctgcct ttggcaaaaa aagcgtgtca cgaaatatca
    12481 aggttcacgc aagtcgtgta attttgctca atgagggtgg cgactttaat tcccctcatt
    12541 ctggagttcc tctgcttgag gcgggttaca acaagctagt tgatctcgaa aaaacatcgg
    12601 gcggcagcgc cgaaggtttt ctgaaaaacg caagccgaca attagggatc aaattaacaa
    12661 aagatgttga tttgagacag ctcgaagaca gcgcaaaagc ccttgggttt tcttcatttt
    12721 ctgatgcgtt gaacgacaag atcaagaaaa tcaactcagg aacagactca gccttgatta
    12781 ctcacgaagg tgatgcctct gtgttgtcag tcgcaccagc agatccgaag ccaacgtggg
    12841 aaatttctgc aaatgagttt gccgcctcag tacaaatccc tttcacgatt ttgttcggac
    12901 agcaaacagg gcgattggca agtgacgagg acaaaaccga ctgggcaaac cgttgcaacg
    12961 gtcgtcgaaa tggttttctc accgatgtga taacacaact acttaatcgc ttgtggttca
    13021 tcggtgtgct acctatgcca aaaaatagcg atgtcacagt gtcttggtct gacttacttg
    13081 caccaagcga aaaagagaaa atcgcaaacg cacaggcttt ggcttcagtt gcaactacat
    13141 cgcagtctgc ctttggtttt gccgcggtaa cagcgaacga aatccgcgaa tctttagggt
    13201 ttgaacctct gcctgataat ctgattccgc caacaattga cgacgaaaat gaagatcaag
    13261 acgaaaatca agccactgcg gattccgaca aataaagccg atccgctcaa tatgggagtg
    13321 tcggttgcca agctatttcg tcacattgat gtcatttact cacaaattaa gcgctccatt
    13381 aagcaacaaa tcttgcaaaa gatgtcactg cgactgcaag caaataattt tgagtatttt
    13441 ccttcgtatc ttactgcgac tgagttggcg gatttgcttg aaaccttgca aaagattatc
    13501 gacaaagaga ttttgtcgaa tggttctcaa ggcgaaaatc tctggtttga ctcctatatc
    13561 gacgaagcag cactaaaagg cactcagtct gcggtcactg atttaggtct gcaatcagag
    13621 gcttatcgca gtcagcgaaa cttatcatca atcgttttca gtcctgcgta cttcaaccga
    13681 ttggcgattg cacacatggc aagttatagc gagtggcaag gtttaagcga cgagttgcga
    13741 aaatctttgg cgggggtgat cacggaggcg gttttaaacg gcaaaaacgt gaaagaaact
    13801 gcccgtgaga ttcggcaaaa acttgatgtg tcatcaaaac gggcgaagcg aatagcgcaa
    13861 tcaagccagt tggcggcata tcgtcgggca gaatggcaag aggcggaaga ggcaaaagaa
    13921 gaacttgggc taaacaccaa gctcttacat ttttcagcgc tcaaagctac tacgcgatta
    13981 gcacacgccc aacgccacgg caaatacttc gatgttgcag aggtgcgcga atggtatagc
    14041 cgagacggca accaattcaa ctgttactgc aagcagtctg tggttgtggt caacgccgac
    14101 ggtaaaacag atattgagcc tctccttgtt gggttggcta aagaacgtaa acaatgggta
    14161 ggagcattaa aaaatgtcaa aaaataacgt gaacatcgtc accgtcatta acagcaaaaa
    14221 tatcagcaca gaatctattg acggagatca acacctgatt atccgcggcg ttgtgccgat
    14281 tgttgatgat gttgtgatga acgacggact ttatcctgcg gatgaaatca ataaaagcta
    14341 caacagctta gagggcaact ttatgccgct tgggcatcca aaaatcgaag gtaaatacgt
    14401 ttcagcccaa gatgttcgag cggtgaacaa acatcacgtc ggtgcgtggg cgaagaacgt
    14461 acgcaaggaa agtggcaaag tgctggtcga tatgtatatc aaccgccgct ttgcggaagg
    14521 ttcagaacag ggcaaaacct tgcttgaacg acttgacgga atggtgacaa acaccaacgc
    14581 tgaacctatt cacgtttcca cagggctgat tctaaacaaa gtcaagcaat cgggtaaatc
    14641 caaaggcaag aaatatagct ggattgcgac aaatatgtcg ttcgatcata ttgcaatatt
    14701 gcttgatgtg ccgggcgcgg cgacacctga tgatggcgtg gggatttttg taaaccaaga
    14761 cggaagcgaa atgggggtgg aaaatgtaaa cctaacacaa gcggcggatt accgcacaga
    14821 aaagctgttt gacaaagtac gattttacct gatggcgaac tcagatttat cgtttgaaga
    14881 aattcatcgc gcgctgcgtg aagctattcg ccccgaaggc aaagataagt ggtcattttg
    14941 ggtcgaagct gtttacccga aatacttcat ctattcggac gataacagcg gcaagaaata
    15001 caagcggagc tattttattg acgaaaatga caatgtgcag ctcactagcg aacctgtcga
    15061 agttgtcaag aagattggct acgaagaaat tacaaccaat gaggaacata atccaatgaa
    15121 aaccaaaatc ttagccgtgt tgaatgcggc aaacgtgaaa acggaaggct taaatgacga
    15181 tcagctcttg gaagcctaca acaagctgca agcggataag ggtaaagagc ctgaaaagcc
    15241 taaaaatgaa gatgacagcg aaattgatga gaaaatcaag aaagcagtca atgccgcgat
    15301 tgagccactc cagcaagcct tgcaagctaa tactgacaaa gagcaagcag caatgcgtgc
    15361 ggcggtgaaa gccaagttca atatgagcga tgccgcagtc aatgctttgc aaggcgaggt
    15421 attaactgag ttgtatgcgc aaacgcaaca atcggtcgga ttaaacaata attcggcaaa
    15481 cggcaacggc gacaatcaat gggatggtta ctcactcaac caagaggaga aaggcaatga
    15541 gtaatgtaat ttatcgcggt tcggtcaagc gtgaacctca aacggtgaat atcgtgatca
    15601 acgatacttc ggcaccgggt gcagtagtta aactccacaa cggcaaatta gaggcggcgg
    15661 ctgatttaaa aggtcgccgt tttttattgg gcaacaaccg ctttaagggg cagacaatcg
    15721 aacaagcgta tgcgaagggc gatactgcaa cggcgtttcg tttagagcct gaacaggagt
    15781 attacgcgca aattgccgac ggcacttata actttggcga tgaactcacg gtaaaacaag
    15841 cgggcggcaa actgaccaaa gcggagacgg gcgatgtagt gctatttttc ttcgacgaag
    15901 aatcgcagcg tgtaatcagt ggcggcaaag gctatgccga tgtcgtggtg gcaaacgcct
    15961 acactaaata agaggtaaca aagcaatgtt gaaattcaca aaagaacaag aacgcttcgt
    16021 gattaacgag cggatcaatt acgacaaaaa acacgcgcta atggcagcaa attcggcggg
    16081 gttattgggc aacgcgatga ctttgccgaa agatgtctgg gcggaatggg atcgtgatgc
    16141 agtgcaaatt cagcgcgaag aattggtggt gtttaacgat ttagcgggca tttcaaaatc
    16201 aatgccgatt ggcaaactta ttcaccattt ccaaacggta tctgatagcg gttcggttaa
    16261 tatctcatta gacggtcgtt catcagcgaa aaccgataac gtggtgctta actaccacgg
    16321 tacaccgttg ccgattattg attccacttt ctcttttggc tggcgcgata tgtcggcagc
    16381 tcaaagcgaa gggtatcaga tcgacggtgc agcacgcgcg aacagtctcc gcaaggtggc
    16441 tgaaaaaatg gaagatctcg ctttaaatgg tgattcctct attgtggttg gcaatgccaa
    16501 gctttacggc ttgcgtactg cgccaaatcg tatgactaac acacacaatc tggatttagc
    16561 cactgcgaca ccgaaagaaa tttacgatgt gttccgtgac ttgattagca aattccacgc
    16621 gaagaactac tactctcctg taacgcttta tgtgaactat ggcgactatt tcgcaatgag
    16681 tacacgtgat tactccgagc agaaatccga aggatctatc ttgaacaaag tgatgacgat
    16741 tccgcaaatt gcgaaaatcg tgccagcaag ccgtgtaccg caaaatgaaa ttctcggctt
    16801 gtgcaagcgt tcggatgtgt accaagtgct taacggtatg ccgttagtga ctcgtccgat
    16861 tgcgcgccat aatgaaaccg atgattactc gttccaaatt atggcagccg ttgccgtgga
    16921 gttcaaattc gacgataaag gcaatgctgg ctatatccag tacactaaat cctaaggcgg
    16981 tgcaaaatga agtggctatt aacacacgat agccacgagt tgaagaaagg cgatgtttac
    17041 gaaggcgaaa cattaccgct ttggcttgtg ggcaaagcta tcgaaatcga agaaaaatcc
    17101 tttgaagttg caacgccttc tgatcacgct gacggcgaaa ctggcgaaac tggcgaaact
    17161 ggcgaaactg gcgaaactgg cgaaactggc gaaactggcg aaactggtca gaaaggcaaa
    17221 aaagataaag gcaaataact atggcagcag aaatagagct atccgaagcg cgaacaacgc
    17281 ttgacgaact gggtttcact tcgccgaact ctctgcttga gctttacgtt cagcaaacga
    17341 acaccttaga ccaggcgttg aacaaatcgg ggtacagcga agaaacacag aaactgatca
    17401 agctctatct ggttgctatt cttgcaattt catctggggc gcgccgtata aagtcagaag
    17461 gtgcgcctag cggtgcaagc cgaacgtttg agtatgatcc aaacgcgcta gacaacctta
    17521 agtcggcgat tcgctcaatt gatccgaaag gttgcgctga aagtttgcta cccaaagata
    17581 aagttgtcgg ctttttcgat gtggtcggag gctgctaatg tcaaatttat cgaattggtc
    17641 ctacaccgca aaagccacgc tttggcaggc taaaggaaag aacgatgacg gcgttttagt
    17701 tttttctacg ccacaaatca tttcctgtga ctacggtgca gatagaaagc gcgcacgttt
    17761 tgagattggg cgtgaacaag cggtcaaaaa tgtgatctgg acggagttcg cccacgccaa
    17821 attaggcgat tatgttctga ttggcgaaag cgagcaagcc gatccgattt tggctggcgc
    17881 agaggaaatt attcatatac agcgctttgc tgatacgttc gaccgcaatc gtgatgattt
    17941 tgcgctgata acaggcgggt aaaatgggga tcaaagtaaa aggcataaga caggtcaagc
    18001 agcggctgaa tcaggttgtt ggcgaggttc agagccaaaa agcaactcgg gcaatgtttc
    18061 gcattttgaa tgcggttgcc cctcttgcgg cgcactatac gcctgttgat acctcaacgt
    18121 tgatcaacag tcaattcact gaaatggaag tgaacggcac acgcttgatt ggaagagtgg
    18181 gttattctgc taattatgcg gcttacgttc acgatcctaa tatcaagcaa aacttccgca
    18241 agccttcagc gaagaaagag tttttgactt ccgcgctgaa cgaatcaaag gctacgatca
    18301 aatccattgt taaagaggag ttgtcactat gatttcttat gtcaaagcct tcaaacaatg
    18361 gttagaggct cactctcttg cagacggcta tgttgtgcag ctttaccagt gggaggatac
    18421 gcaaaaagcc aagcctgtta ttgttattca gccaaatagt ggttcgccgc aagtttctga
    18481 cctgagcagc gaacactatc tgctaattag ccttgttgca ggtaaaaatt caggttacac
    18541 aatcgaagag cgagcaaggg cgattatgtc aaagatttta gttgagcctt tcgcttcctt
    18601 tggttacatc gaatcaatgg gcggattacc cgccccaatt ttcaccgaag ataatcgaat
    18661 gatttttcgc ttaccacttc ggattatttc aacaaatcaa gaggagtagt ttatgggttc
    18721 tccatctcca caaaataacg cagaaaatct cgtggtgggg cgtgccgtag ttttagagta
    18781 cggtacgact gatgcaaagc cacaagaaag cgaatggaag gttgcgggcg caatgacgac
    18841 aaagagttgg gattttagcc caaattcggt tacatcagag gctgatgatg ctggcggctt
    18901 ccctgaaacc ttaattacaa actcagactt tagtatttct ggtgacggtg agtggcgcaa
    18961 acgaccaaaa tcgaaagaac taggcatcaa agatctagtt gtgttatacg caaatgcggt
    19021 aaaaaatcgc acccaacctt acatttgggt tcgtttgaaa tacgcaggct taacaattct
    19081 cggaaaaatg gttatcactg cattaagtag cgaagcccca accaatgact tagtgaaatt
    19141 ctctatagag ttcaaggttg gcgattcaag cactatcgaa attgctgcag cgtaaaaaat
    19201 cccaccctct ataacaagag cggtgggatt atcattatcc tagggatagt tgtaggggct
    19261 ttcctaagct agccaaaata cgaccaatag tgtcgatttt tgtaatatgg cgaggggcta
    19321 aaattcgctg tacttctggc ggacggatat tagttaatcg ggcaatttct gccttgctta
    19381 ccgcttgttc ttgcattgta ttatgcaaaa gcacttttgc gtagatgctc tctggcataa
    19441 acacaggcac ttcgtcggta tttactgggc gagtgagcgg aaagcgtcta tcttcatcga
    19501 aataaatttc aacacaagat agtagcacgt cttctgccat ttccattgct tcttcaaatg
    19561 tatcgccttg ggtgatagct tctggcaaat caggaaatgt cactacaaag cccccttctt
    19621 cagcgggggt aaataacgct ggataaaaca tattttctcc ttattcaagg gtgtagtgaa
    19681 agccccttaa catcaggggc tttatattat tttaagccta gttgcttttt aaccccttcc
    19741 actaagcctg tttttaactc ttggcttggg tgtttgggga ggtggcttct tttgcccttg
    19801 tagtagagtt tcaaatgctt tgatccattt tcaacctcta ccccattagc ctttagccac
    19861 cttaggaatt cgctttgctt cactacctcc tcctatttgt ttaacttggg ttcattatat
    19921 gcaaaaatgc taactttgtc aacagaaagt tagcaaaaat gctaattttt ttgttgaggt
    19981 gaaaatggtt agctatccaa ttactgaaat tggtgaaatg cttattgcaa ccgatgggcg
    20041 agacttttta ttcagaccga gtttcaaaaa tatctacaaa attggaagcc cacaagaaat
    20101 tgtaaaaatc tatgcactat tgcacggctg tgagattcga gagatattgc cgcacatttt
    20161 aaccaaatcg gagtctcatc aatcttactt ttggtcagtg ctgaattcgc ctgtttttgg
    20221 cagaaaaatt ctgcaaaacg caatgatcgt gatgtcgtgc tgttgcgatg atgatatttc
    20281 cctgttgatt ggcgagtggt tgccgtcaaa gtctggcgtt aggtacaagt tgggtaaaat
    20341 gccacctgag acaattatca attttgcaaa aaatttaatg ggtcacggag tgattggttg
    20401 ctgcgaattg ccaaagaaag aacaatctgc aaaaaatgaa ttttcagaaa gttttgatgc
    20461 tgttgagtac ataagtttag cccgaactca tttcaatatg agccgcgaag acgctgaaaa
    20521 tctcacgatg accgaattgc agcaacttct aaaaagtcaa atgcctgccg aaaataatag
    20581 ctatggacag ttcaccgaag aagagtacga cagaattatg gcggaatatg agaaaagaag
    20641 agcggcttaa atagccgcta acatttaaaa gaacctacct gatagaacgg gagtgaaatc
    20701 cttgtaatgt gctgaataaa aaagctctct tatgtcattg agtagctcaa attcatcttt
    20761 ggatcttgcg ttgcagattg ccacatagtc atctacgcag gataaaatac gttcaatact
    20821 gacttctttt atccagcata cttcttggtt ttcattcagc cagttggcaa agtcttgttt
    20881 agtgcagtct tttttcataa gttctccttt ttgattattt gcaaaaaaaa tcatatcaaa
    20941 tcatccgttg ttatctattg gcgaaaataa tttttgcgac acagatcgca aaaataggtt
    21001 ttgacggtgg aaaaacgaat cttaattcgt agaatggtaa aatttcttta tttttaaggt
    21061 atttttatga aaaaaatgat tgtttttgta gcttctgttt tgtcatcttt tgttattgca
    21121 tcggattggg aaataataga agcggctcaa aataaggtga aagcagagct tataagaaaa
    21181 tcagaaaaaa aagcagagtt atgtttaaat gttgctcagg ctgtcttaat tactaatcaa
    21241 aaagtacaaa aagatacttt ctcaatgtgt ggtgaatttt cgacagagat tgtcttttct
    21301 gactctaaag catatagcct tgcagtatgt ggaaaggtta aaggtaaaga tattatcgga
    21361 aatccgatag agagcgacta tatataccaa agagcaacta aggattttta ctttaattat
    21421 aatggcaaaa aatcagctta ttatgatcct caatctgatc ttgcaaggct tcaagaggt
//
LOCUS       DANLRK010000017        21357 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.017, whole genome shotgun sequence.
ACCESSION   DANLRK010000017 DANLRK010000000
VERSION     DANLRK010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 21357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21357
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.017"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(68..2980)
                     /gene="rapA"
                     /locus_tag="PXA33_000516"
     CDS             complement(68..2980)
                     /gene="rapA"
                     /locus_tag="PXA33_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HDL5119880.1"
                     /translation="MFLVGQRWLSESENNLGLGIVTSVDHRTVTINFPAAEEERVYAI
                     AVAPLTRVQFQAGDRINSIDGWQLEVSEVVENQGAVIYLGKRVDNGEEAVLPEMLLDH
                     KISFSKPQDRLFSAQIDRSDRFALRFNALQHQQAQYQSPLRGLRGIRASLIPHQLHIA
                     KEVGQRLAPRVLLADEVGLGKTIEAGMILQQQLFSGRAERVLVLVPESLQHQWLVEML
                     RRFNLKFSLFDEERCSDFDKQDENGDDVSENPFESEALVITSLNWLETSPQRANQVLA
                     ADWDLLIVDEAHHLEWSETESSIGYQLVERLSQQIPAVLLLTATPEQLGQESHFARLA
                     LLDSDRFYDYAEFVQEQAQYKPVADAVSSLLSDKPLSNDEQNAIAELLSEQDTEPMFR
                     AINSKKSDENDRLQARQELISELIDRHGTSRVLFRNTRQGVKGFPHREYHQISLEMPK
                     QYQNALKVMGMMGSVSQSDLLYPEYLFQRMNPNAKWAEFDPRIDWLITFLKNHRDEKV
                     LVICKQADTAIALEQVLREREAIRSAVFHEKMSIVERDKAAAYFAQQEEGAQVLISSS
                     IGSEGRNFQFAKDLVLFNLPDNPDLLEQSIGRLDRIGQKNDIQIYVPCFNLSAQQMLA
                     EWYHKGLNAFEETCPMGTAIFRELGEELQQFLQKPELSAQFDDFVAKTYKRQQYLKAE
                     LEKGQDRLLELNSNGGEAAQQLANDIAKEDNNPQLVNFALSLFDVIGVEQEDLGEQSI
                     VISPTGHMLVPDFPGLSEDGTTITFNRELALMREEVEFLTWDHPMIRNGIDLITSGDI
                     GKSAISLLVNNKLPAGTLLLEALYVVEAQAPKGLNLTRFLPPTPIRVLVDNKGNNIAP
                     QVSFAGLERQLKPVNKQMANKIAKMARADIEKLIKASEKAVEPQVQQVIETAKFEANS
                     KLKAELHRLESLKMVNKNIRADEVEALEQQLNASLAQLETANYRLDSLRVIVSNKA"
     gene            complement(3141..3440)
                     /locus_tag="PXA33_000517"
     CDS             complement(3141..3440)
                     /locus_tag="PXA33_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5119881.1"
                     /translation="MRFLPSEIYQPRGELVKADRQGNGEFEVEYRVSGNDVRGLAKSA
                     IAHAKRKGFHLVESDIHRDDADLKFKRGDQELDIEIEVKGRNRIEYKADLDLDKN"
     gene            complement(3561..4886)
                     /locus_tag="PXA33_000518"
     CDS             complement(3561..4886)
                     /locus_tag="PXA33_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase
                     family protein"
                     /protein_id="HDL5119882.1"
                     /translation="MISSVWNERYLADKKREQDHRSPFQRDRARLLHSEAFRCLQAKT
                     QIHAVGEDDFYRTRLTHSLEVAQIGSSLREKLLADVEGFQAVCSEQEFAKNMANLTAL
                     LPDHSLVESLCFAHDLGHPPFGHGGEMALNYKMYAFGGFEGNAQSFRIMTQLEPYTET
                     AGMNLTRRTLLGVMKYPALLSQTSPIEPAEMSEARHINLHHFPQSKGIFDDNNHFFEW
                     VLAPLSEKDRRQFCTVQPNQDPKQPNKTQYKSLDCSIMELADDIAYGVHDLEDAIVGG
                     MVTPQSWQNAEKLLAECQSDWVKQRLPEIREKLFSQHRYERKDVIGALVNHFITNVRW
                     KALPEFDEPLLRYNAYLPESVACVLKILKDFVYQYVICDAKTQRVERKGQRILMELFD
                     ILSSDPMRLLPNNVRERWKNAEESKRARVICDYLAGMSDGQAFKLFESL"
     gene            4971..6290
                     /gene="rlmD"
                     /locus_tag="PXA33_000519"
     CDS             4971..6290
                     /gene="rlmD"
                     /locus_tag="PXA33_000519"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDL5119883.1"
                     /translation="MAIFYSEKSAKKPIKSPACQKATIQSLDYQGLGIAKINGKTWFI
                     ENALPDEQVEFKVLEEKRQYGRGQAVKILKKSANRRMPSCELYGQCGGCQMQHISLEL
                     QRKAKQQALFQRLQKLQTHAIDVQPMIIGNDKHYRRRAKLSIAIQQGKAVIGFRLQNS
                     NQIIPLAHCEVLVEPLANLLPKLKHLIENWQQPKKLGHIELVSADNTIALFFRHLGAL
                     SAQDNHNLRQFATQEQLSLFVMTSENHIEHLCGERPYYQIQGLKLHFSIRDFIQVNAT
                     LNEKMVEKALEWLELSNQDRVLDLFCGMGNFTLPIAERAKSVVGVEGVEPMVQQAREN
                     AVTSGLKNVEFYQTNLDEPFSDKPWAKEPFNKVLLDPARNGALFCLDHLTSLNPERIV
                     YVSCNPATLVRDAEKLLQLGYRLEKAAMIDMFPHTGHLESISLFLKS"
     gene            complement(6430..7611)
                     /locus_tag="PXA33_000520"
     CDS             complement(6430..7611)
                     /locus_tag="PXA33_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HDL5119884.1"
                     /translation="MESRRPSLSFFIILGMMAMLPPLAIDMYLPSFLDIARDLAVSQE
                     KVQTTLAVFTLGFGAGQLFWGPMADSFGRKIIILIGLVGSAIAAYFLTQVQNIETFYL
                     LRLVQGLCAAAPAVVLGALVRDLFERNLFAKLMSVIMIISMLAPLLAPIVGGYIAKYF
                     HWHSIFYVLVAMGILCVVLISWKIPETLAPEKRLPLSFGKTLSNFAILLKHRPTLGYV
                     LIGGLTFAGIFCFLTSGSIVYIGLYGVSQENFGYFFGLNMIVMVTMTAINSKMVVKVG
                     SETMLRIALAMQLLAGIWLALVAIFDLGFWAMAIGIPLYIGMLSTIGSNASAAILEQF
                     PQMAGTANGVAGTARFGVASLVGAGLSHIAVTSAAPMLLAMALCVMLAGVVYYFLCYK
                     R"
     gene            complement(7601..8302)
                     /gene="rsuA"
                     /locus_tag="PXA33_000521"
     CDS             complement(7601..8302)
                     /gene="rsuA"
                     /locus_tag="PXA33_000521"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HDL5119885.1"
                     /translation="MRLDKFIAENTGLTRSQAAKVLKSGVVTVNGNIEKSGAAKISQE
                     DEIYYEDQKLEWVEEGQYFMLYKPQGYICSHDDGEYPTVFQFFDYPLMTKLHTAGRLD
                     VDTTGLVLLTDDGKWSHRITSPKHHCEKTYLVTLADPVEDFYAQRLAEGILLRGEKEP
                     TLPAQLEILDDYNVNLTISEGRYHQVKRMFAALGNKVEALHRWRIGDVVLDERLEEGE
                     FRSLTSEEIASFNGK"
     gene            complement(8547..9518)
                     /gene="menC"
                     /locus_tag="PXA33_000522"
     CDS             complement(8547..9518)
                     /gene="menC"
                     /locus_tag="PXA33_000522"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HDL5119886.1"
                     /translation="MRQVKLYRYQLPIQTGVVLRKQPLKERQGVLVCLQEQEKVGWGE
                     IAPLPSFSQESLEQAENQAVNWLKAWQKGEPETLEQLFPSVAFGLSCALAELNNELGE
                     QANNQSAVLCYGDLEKLHTAFSQHTNPIGKIKIGLNPEQEGEQANLLLHTFPNLRLRL
                     DANRLWSLEQAVKFAEKIANEHKPRIQFIEEPCQALELSRQFATQTDIAIAWDETVRE
                     ANFLVKKEPNLTAIIIKPTLVGSLEKCVNLIQQAQKQGLVAVISSSLESSLGLTQLAR
                     IAQQYTPNSVPGLDTLNLMQHQLLRDWHDSSLPLVGLESEFVREVVV"
     gene            9730..11808
                     /gene="metG"
                     /locus_tag="PXA33_000523"
     CDS             9730..11808
                     /gene="metG"
                     /locus_tag="PXA33_000523"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HDL5119887.1"
                     /translation="MANQKRKMLVTCALPYANGAIHLGHMLEHIQADIWVRFQRMRGN
                     EVHFVCADDAHGTPIMLNADKLGITPEQLIEKAKADHEADFASFNISFDNYHSTHSEE
                     NRQITTDIYKTLKANGFIKSKVISQLFDPEKNMFLPDRFVKGTCPKCKAEDQYGDNCE
                     VCASTYSPMDLINPRSAVSGATPVVKESEHFFFDLPSFEGMLKEWTRSGSLQPEIANK
                     MQEWFESGLQQWDISRDAPYFGFEIPDAENKFFYVWLDAPIGYIASFKNLCDRAGIDF
                     DEFWQKDSTTELYHFIGKDIVYFHSLFWPAMLEGSGYRKPTNVFAHGYVTVDGAKMSK
                     SRGTFIQANTYLKHLDPECLRYYYAAKLNDRIEDLDFNLDDFVQRVNSDIVNKLVNLA
                     SRNASFIAKRFEGKLAEKLDDEALFNEFTAQAETIANFYENREFNKAIRAIMELTDKA
                     NKYIDDKAPWVIAKEEGKEAELQAVCSMGIELFRVLMSYLKPVLPKLAERAEAFLQSE
                     LRWDNIATPLFGHTVAPFKSLFSRLEKKQIDAVIDETKALFAEANKKTENQTKGTQKV
                     EQQAVEKQENIAVEPISPEITIDDFAKLDLRAAKVLNCEAVPKSDKMLRFELDLGDHQ
                     RQVFSGIKAAYPNPEALIGRFVIVIANLAPRKMSFGMSEGMILSAGTGGANLFLLDAD
                     SGVQPGMQVK"
     gene            11818..12234
                     /gene="ndk"
                     /locus_tag="PXA33_000524"
     CDS             11818..12234
                     /gene="ndk"
                     /locus_tag="PXA33_000524"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HDL5119888.1"
                     /translation="MIQQTLSIIKPDATRRNLIGKILAQLEENGLTIKALKKVHLTRE
                     QAEGFYAEHKGKDFFEPLVEFMTSGSIVVSVLEGENAISHYRALMGATNPDNREKGTI
                     RDLYALSMRENSVHGSDSEESAKREIAYFFVPSEIV"
     gene            complement(12290..12766)
                     /gene="greA"
                     /locus_tag="PXA33_000525"
     CDS             complement(12290..12766)
                     /gene="greA"
                     /locus_tag="PXA33_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HDL5119889.1"
                     /translation="MKQIPMTVRGAEQLREELDFLKNVRRPQIIDAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIQEIEGKLGNAQIIDVTKMANNGKVIFGATVQLVNADTDEE
                     VTYRIVGDDEANIKEGLISVNSPIARGLIGKEVDDSVSITTPGGKVEFDIVGVEYI"
     gene            12863..14308
                     /gene="dacB"
                     /locus_tag="PXA33_000526"
     CDS             12863..14308
                     /gene="dacB"
                     /locus_tag="PXA33_000526"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005615286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HDL5119890.1"
                     /translation="MIFKNFTRAFLNKFILSTLLIPSITQAEINTEELLNTLPAGTSV
                     SFIAKNLDTNQIITQYQSDIFMLPASTQKVFTALAAKLTLNDDFRFQTALLTNGKVEN
                     GVLKGNLIARFSGDPELTSGQIYQLMSKLKQQGINKIEGDLILDPSVFASHDKASGWI
                     WNDLTMCFNAPPAAINVDHNCFYVTLNADQPIGEFAKVNVPSAYPVQVFSSAYIVEPK
                     EAPFCQLDVVVHDNNRYQIKGCMARQSQPFGLSFSVQDPTNYGANMLKAQLKSLKIAF
                     NGQVKEPLTAQNGTLLAEHYSEPLPVLLKKMMKKSDNQIADALFRTVANKQHNRPASF
                     QLGSYVIRQLLKTKANIDFKNSVVADGSGLSRHNQVSSRTMLETLEYIAQNEESLKLF
                     ETFPIAGVDGTISGRGSISTEPLAKNLIAKTGSLKGVYNLAGFMKNARGERIAFVQFI
                     NGYSTGELESKTKRAPLNTFENKFYMALFNE"
     gene            complement(14395..14664)
                     /locus_tag="PXA33_000527"
     CDS             complement(14395..14664)
                     /locus_tag="PXA33_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDL5119891.1"
                     /translation="MINHNLADFVFEYFTENNLKAGTLRYRYIKENVIDAYSTRVDDA
                     FQGKGIAGELYNALIAFAQEKGLKIKPSCSYIEVKMQRSHRDLIA"
     gene            14836..16425
                     /gene="purH"
                     /locus_tag="PXA33_000528"
     CDS             14836..16425
                     /gene="purH"
                     /locus_tag="PXA33_000528"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005821463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HDL5119892.1"
                     /translation="MAIQQALLSVSDKKGIVEFARGLSARGVKLLSTGGTAKLLAEAG
                     LLVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGTDDEVMNQHGIERIDMVVVNLY
                     PFAQTVANPNCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVNNNDFDAILAEMDQHQ
                     NSLTLETRFNLAIKAFEHTAQYDSMIANYFGQKVPPYQGAEEEDLTQPSGQFPRTLNL
                     NFVRKQTMRYGENSHQNAAFYVENELKEASVSTAKQLQGKALSYNNIADTDAALECVK
                     EFSEPACVIVKHANPCGVALGKNILDAYNRAYQTDPTSAFGGIIAFNRELDGETAQTI
                     ADRQFVEVIIAPTVSASAKEVLATKKNVRVLECGEFSEAVKRLDFKRVNGGLLVQDAD
                     QGSVSIDDLKVVSERKPTEAELKDLLFCWKVAKYVKSNAIVYAKDGQTIGIGAGQMSR
                     VYSAKIAGIKAEDEGLQVVGCVMASDAFFPFRDGIDAAAKVGITCVIHPGGSMRDQEV
                     IDAANEHGMAVVLTGMRHFRH"
     gene            complement(16480..17856)
                     /locus_tag="PXA33_000529"
     CDS             complement(16480..17856)
                     /locus_tag="PXA33_000529"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000626422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HDL5119893.1"
                     /translation="MISVFDMFKVGIGPSSSHTAGPMKAAKEFVDELQNRKFLNKIDR
                     LRVDVYGSLSLTGRGHNTDIAIIMGLMGYLPHNVDIERIEQTIENLKQNKQLIIAESS
                     VEPKVIAFDFYEDMPFHRDFLPRHENGVKFSALSGEELLFQNTYYSIGGGFIVDEANF
                     EQVKNSDLSVPFPYQNAADLLAHCKNNGLPLSSLVWKNELALRSRVEISDYLAMIWNT
                     MEACIERGLNTEGLLPGPLKVVRRASGLRRMLEANSKFNTDPMQIIDWVNMFALAVNE
                     ENAAGGRVVTAPTNGACGIVPAVLAYYQKFIAQLNQDTIERYLLTTSVIGSLYKMNAS
                     ISGAEVGCQGEVGVACSMAAAGLTEIMGGSAEQVCMAAEIAMEHNLGLTCDPVGGQVQ
                     VPCIERNAIASVKAINASRMALRRTSSPQVSLDKVIETMYETGKDMNAKYRETSTGGL
                     AIKILPCD"
     gene            complement(17913..19139)
                     /locus_tag="PXA33_000530"
     CDS             complement(17913..19139)
                     /locus_tag="PXA33_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HDL5119894.1"
                     /translation="MAMPSQRFSLTWVLNLFGTAVGAGVLFLPINAGMGGFYPLIIMT
                     LLVGPMTYLAHRGLTRFVLSSKYKGSDITAVVREHFGEQAGKLITLLYFFAIFPILLI
                     YGVGITNTVSSFMENQLHIAPPSRAVLSFMLIAAMIGVMLLSEQVMLKITTCLVYPLV
                     LILLGLSIYLIPQWNTAALQQMPTTGDFLTTLWLTIPVLVFAFNHSPAISSFALSQQK
                     YYQDDKKAEIESAKVLRSTAFILVLFVMFFVFSCVLTLTPEELAQAKVQNISILSYLA
                     NKFDNPIISYFGPLVAFLAIGSSFFGHYLGAREGLEGLVNQMRKEPIDPSKFRKITAI
                     TFLIILWIVATINPSILGFIESLGGPIIAMILFIMPVYAVYKVPALARFKGEFGHLFV
                     LVMGCIAISAIVYGLL"
     gene            complement(19409..20296)
                     /gene="dapA"
                     /locus_tag="PXA33_000531"
     CDS             complement(19409..20296)
                     /gene="dapA"
                     /locus_tag="PXA33_000531"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDL5119895.1"
                     /translation="MATPLFHGSIVALVTPMTHGEVDYQDLKKLVEYHIQAGSHAIVS
                     VGTTGESTTLSIDENVKVIKKTVEFADGRIPIIAGTGSNATSEAITMTKLLANSGVAG
                     CLSVVPYYNKPTQEGMYLHYKAIAESTDLPQILYNVPGRTGSDLKPETIGRLAKIENI
                     VAVKEATGDLSRLPLIKQLAGEDFIFLSGDDATGFESMKLGGQGVISVTNNVAAADMA
                     KMCELALAGQFTEAEEINNRLMALHHDLFVESNPIPVKWAAYKLGLISEPNLRLPLTT
                     LSESAQPVVLKALQQAGLI"
     gene            complement(20392..21333)
                     /locus_tag="PXA33_000532"
     CDS             complement(20392..21333)
                     /locus_tag="PXA33_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxL/LpxP family Kdo(2)-lipid IV(A)
                     lauroyl/palmitoleoyl acyltransferasee"
                     /protein_id="HDL5119896.1"
                     /translation="MSEKKQLPPFQTQFLHPKYWGLWLGLGVFKLILCLPYPILVKIG
                     LGLGKLFTKLGFGKRRMRIARRNLELCFPHYSAEKVEELVEENMKSVGMAIIETGMAW
                     FWSDKRILKWSKIEGLEHLTQRDQERGILLVGVHFLTLELGARIIGLHHQGVGVYRPN
                     DNPLLDWIQFRGRVRSNKAMLDRKDVRGMIRALKNGEMIWYAPDHDYGRRNSVFVPFF
                     AVEETCTTAGSYMLLRSAPKSLVIPFSPIRNLDYSGYTVKVSPAVDFSQCQNEVETAI
                     LMNRVVEREIMAALTQYMWLHRRFKTRPNEGDQSLYD"
BASE COUNT         6362 a         4851 c         4129 g         6015 t
ORIGIN      
        1 gacacaaaat agaaaagtga ttttttcgac acagatcgaa aaaatcactt ttcgtacttg
       61 caaaaaatta cgctttatta cttacaatca ctcgcaagct atctaagcga taatttgccg
      121 tttcaagttg ggcaagggaa gcgttaagct gttgttctaa ggcttcaact tcatcggctc
      181 gaatattttt attcaccatt ttgagtgatt ccaagcggtg taattctgct tttaatttgc
      241 tatttgcctc aaatttggca gtttcaatca cctgttggac ctgaggttct accgcttttt
      301 cactcgcttt aattaatttt tcaatatccg ctcgagccat tttggcaatt ttatttgcca
      361 tctgtttatt caccggtttt aattggcgtt caagcccggc aaaagagacc tgcggagcaa
      421 tattgttgcc tttgttatcc actaaaaccc gaatcggggt tggcggtagg aaacgggtta
      481 aatttagccc tttcggtgct tgtgcttcca ccacataaag cgcctcaagt aataatgtac
      541 cggcaggcaa tttattattc accagcaatg agattgctga tttgccaata tcgccggagg
      601 tgattaaatc aataccattg cggatcatcg ggtgatccca agtgagaaat tccacctctt
      661 cccgcattaa cgccagttca cgattaaagg taatggtcgt gccatcttcc gatagccccg
      721 ggaaatcagg cacaagcata tgtccggttg ggctaatcac aatgctctgc tcgcctaaat
      781 cttcctgttc aacgccaatc acatcaaaca aactcaaggc aaaattaacc aactgcggat
      841 tattatcttc tttcgcaata tcattagcga gctgttgagc tgcttctccg ccattagaat
      901 tcagttctaa taatctgtcc tgtcctttct ccagttcggc tttcaaatac tgttggcgtt
      961 tataggtttt cgcaacaaaa tcatcaaatt gggctgaaag ttcaggtttt tgcaggaatt
     1021 gttgcaattc ttcacctaat tcacggaaga tcgctgttcc catcgggcag gtttcttcaa
     1081 acgcattcaa gcccttgtga taccactctg ccaacatctg ttgagccgat aaattaaagc
     1141 aaggcacata aatctgaatg tcatttttct gtccgatacg atctaatcgc ccaatacttt
     1201 gctccaataa atccgggtta tccggcaaat taaacagcac taaatcttta gcaaattgga
     1261 aatttcgccc ttctgaaccg atgcttgaac taatcagcac ctgagcgcct tcttcttgtt
     1321 gagcaaaata ggcggcagct ttgtcacgtt caacaatcga cattttctcg tggaaaacgg
     1381 cagaacgaat cgcctcacgc tcacgcaaaa cttgctccaa agcaatcgca gtgtccgcct
     1441 gtttgcagat caccagcact ttttcatcac gatgattttt taagaaagtg atgagccaat
     1501 caatccgagg gtcgaattca gcccatttcg cattcgggtt cattcgttgg aacaaatatt
     1561 caggatagag caaatcactt tggctcacac tccccatcat tcccatcact tttaacgcat
     1621 tttggtattg tttcggcatt tctaggctga tttggtggta ttcccgatgt gggaagcctt
     1681 tcactccctg acgggtattt ctaaacaaca cccggctggt tccatggcgg tcaattagct
     1741 cactaatcag ctcttgacgg gcttgcaagc ggtcattttc gtccgatttt ttgctattaa
     1801 ttgcacggaa catcggctcg gtatcttgct cgcttaacag ctccgcaatc gcattctgct
     1861 cgtcattact tagcggttta tcgcttaata agctactcac cgcatctgcc accggtttat
     1921 actgtgcttg ctcctgtaca aactcggcat aatcataaaa acgatccgaa tctaacaacg
     1981 ccaagcgggc aaaatggctc tcttgcccta attgctccgg tgttgccgtt aaaagcaaga
     2041 cggcaggaat ttgttgagat aaacgctcta ccaactgata gccgatagaa gattcggttt
     2101 ccgaccattc taaatggtga gcttcatcaa caattaatag atcccagtcg gcagcaagca
     2161 cttgattggc tcgttgcggt gaggtttcca gccagtttag tgaagtaatg actaacgctt
     2221 cgctctcaaa tggattttca cttacatcat cgccattttc atcttgttta tcaaaatcac
     2281 tacaacgttc ttcatcgaat aacgagaatt tcagattaaa acggcgaagc atttccacca
     2341 accattggtg ttgcaaactt tccggcacta ataccaatac tcgttctgct ctgcccgaaa
     2401 atagctgttg ttgcaaaatc ataccagctt caatagtttt acccagtccc acttcatcgg
     2461 ctaatagcac acgaggagca aggcgttgcc cgacttcttt tgcaatatgt aattgatgtg
     2521 gaatcaggct ggctcgaatg cctcgcaaac cacgcagtgg cgactgatat tgcgcttgct
     2581 ggtgctgcaa agcgttaaag cgtaatgcaa agcgatctga gcgatcaatc tgagccgaaa
     2641 aaaggcggtc ttgcggtttg ctgaaactga ttttatgatc gagcaacatt tcaggcagta
     2701 ccgcttcttc accgttatcc acccttttgc ccaaatagat tacggcacct tgattttcca
     2761 caacctctga aacctctaac tgccagccat ctatactgtt aattctatct ccggcttgaa
     2821 actgcactcg agttaaaggt gcaactgcaa ttgcatagac ccgctcttct tccgctgccg
     2881 ggaaattgat agtgacagtg cgatgatcca ccgaagtcac gattcccaaa cctaaattat
     2941 tttccgattc acttagccaa cgctgaccga ctaaaaacat tttttcctct aagtaataag
     3001 taaaagcatc attattgtca tttaaacggc tattttacca aattccggga ccggtcggct
     3061 tgagtatttt tgcaaaaaag cacaataaaa agaccgcttg tgcggttaaa tcacacaagc
     3121 ggtcgtttta aggggtaaat ttagttttta tctaaatcta gatctgcctt atattcaatc
     3181 cgatttctac cttttacttc aatttcaata tccagctctt ggtcaccacg tttgaatttt
     3241 aaatcagcat catctctatg aatatcagat tctactaaat ggaaaccttt acgtttagcg
     3301 tgggcgatcg cacttttcgc taagccacga acatcattac cactgacacg atattctact
     3361 tcaaactcac cattaccttg acgatctgct ttaactaatt cgccacgagg ctgataaatt
     3421 tcacttggaa gaaaccgcaa atgctgttgc agaagccaaa gttgctaata ctaaaagacc
     3481 tttagaaaca gttttcattg aaatctccta ttaaataaat gaaacctatt actgagacct
     3541 tgcaaaatta acaaagttca ttataaactt tcaaaaagtt taaacgcctg cccatcagac
     3601 ataccggcta aatagtcgca aattaccctt gcacgcttag attcttccgc atttttccaa
     3661 cgttcccgca cattattagg gagtaatcgc atcggatcac tgcttaaaat atcgaacaat
     3721 tccattagaa tgcgttgccc cttgcgttcc acccgctggg ttttggcatc acaaatcaca
     3781 tattgataca caaaatcttt caagattttc agcacacagg caacactttc cggtaaatag
     3841 gcattataac gtagcaacgg ctcgtcaaat tcaggcagag ctttccaacg cacattggta
     3901 ataaaatgat tcactaatgc cccgatcaca tcttttcgct cgtagcggtg ctgagaaaag
     3961 agcttttccc gaatctccgg caggcgttgt ttcacccaat cggattggca ctccgccagc
     4021 aatttttccg cattttgcca cgattgcggt gtgaccattc cacctacaat cgcatcttcc
     4081 aaatcgtgta cgccataggc aatatcgtcc gctagttcca ttatgctgca atcgagagat
     4141 ttatattgcg ttttgtttgg ctgtttcgga tcttgattgg gctgaaccgt gcaaaattgg
     4201 cgtcggtctt tttcacttaa cggggctaat acccactcaa aaaagtggtt gttgtcatca
     4261 aaaatccctt tgctctgtgg aaaatggtgc aaattaatat gccgagcctc gctcatttct
     4321 gccggctcaa tcggcgaggt ttgagacaag agtgccggat atttcattac ccccagtaag
     4381 gtgcgacggg tcagattcat tccggctgtt tcggtgtaag gctcaagctg ggtcataatc
     4441 cgaaaggatt gagcattacc ctcgaagccg ccaaaggcgt acattttata atttaacgcc
     4501 atttcaccac cgtgcccgaa cggagggtgc ccgagatcgt gggcaaaaca aagggattcc
     4561 accaagctat gatccggcaa taaagcggtc agatttgcca tattttttgc aaattcctgc
     4621 tccgaacaga ccgcttgaaa gccttccaca tccgctaata atttttctcg caaactgcta
     4681 ccgatttgag ccacttccaa cgaatgggtt aaacgggttc gataaaaatc atcttccccc
     4741 acggcgtgaa tttgcgtttt cgcctgcaaa cagcgaaaag cctccgaatg caataacctt
     4801 gcccgatccc gctgaaaagg cgagcggtga tcttgttccc gttttttatc cgccaaataa
     4861 cgttcattcc aaaccgatga aatcatattg cctccatttt ccgccaactg cttttcttat
     4921 ataatctccc catttcgaca ctttatatca taacaattca tctgaaaaca atggctattt
     4981 tttattctga aaaatcggca aaaaaaccga taaaatcacc cgcttgtcaa aaggcaacta
     5041 ttcaatcgtt agattatcaa ggcttaggga ttgccaaaat caacggcaaa acttggttta
     5101 tcgaaaatgc cctgcctgat gagcaggtgg aatttaaagt gttggaagag aaacgccaat
     5161 acggacgtgg acaagcggtt aaaattctta aaaaatctgc aaatcggcga atgccaagct
     5221 gcgaacttta cgggcaatgc ggtggctgcc aaatgcagca tatttcgctt gaactgcaac
     5281 gtaaggcaaa acaacaggct ttattccaac gcttacagaa attgcaaacc cacgctattg
     5341 atgttcagcc gatgattatc ggcaacgata aacactaccg ccgtcgggca aaattaagca
     5401 ttgcgatcca gcaaggcaag gcggtaatcg gctttcgttt gcaaaattcc aaccaaatta
     5461 taccgcttgc acactgtgaa gtgctggttg aaccgcttgc caatttgctc ccaaaattaa
     5521 aacacctgat tgaaaactgg caacagccga aaaaattggg gcatattgag ctggtcagtg
     5581 cggacaatac cattgcccta tttttccgcc atttaggggc attatcggcg caagataacc
     5641 acaatttgcg tcaattcgcc acacaagagc aattatctct ttttgtgatg actagcgaaa
     5701 atcacattga gcatctgtgc ggtgaacgcc cgtattatca aattcagggt ctaaagttgc
     5761 atttttcaat tcgggatttt attcaggtga atgccacgct caatgaaaaa atggtggaaa
     5821 aagcccttga gtggctggaa ttatccaacc aagatcgagt gttagatctc ttctgtggaa
     5881 tggggaattt taccttgcct attgctgagc gagcgaaatc ggtggtcggt gtggaagggg
     5941 tagaaccaat ggtgcaacaa gcccgagaga atgcggtgac aagcggtcta aaaaatgtgg
     6001 aattttacca aaccaattta gatgagccgt tttctgacaa accttgggca aaagagccgt
     6061 ttaacaaggt actactcgac ccggcacgca acggagcact attttgccta gatcacttga
     6121 catcactcaa tcctgagcgg attgtttatg tctcttgcaa tccggcaaca ctggtgcgag
     6181 atgccgaaaa actgttgcaa ctaggctaca ggctagaaaa agcggcaatg attgatatgt
     6241 tcccccacac cgggcatttg gaatctattt cgctgttttt aaaatcttag tagatattgc
     6301 cccgatggtg cgagcgtcca cgctcgtacc acacaagcgg ttaattctgc ctaaaaattt
     6361 gcaaaaaatg tactattatt aaccgcttgt agaaatttgt tgggttttcg caagctaacc
     6421 ccaacctagt tatcgcttat aacacagaaa ataatacacc acaccggcta acataacgca
     6481 caatgccatc gccaaaagca ttggggcagc actggttaca gcaatatgtg aaagccctgc
     6541 tccaacaagg gaagcaacac caaaacgagc cgtacctgct actccgttag ccgtgcctgc
     6601 catttgtgga aattgctcta aaatggcggc actggcatta ctaccaatgg tggaaagcat
     6661 tccgatataa agcggaatac cgattgccat tgcccagaag cctaaatcaa aaatcgccac
     6721 taaagccagc caaatgccgg ctaacagttg catagctaaa gcaattcgta acatcgtttc
     6781 agagccgact ttcactacca ttttgctgtt aatcgccgtc atcgtcacca tcacaatcat
     6841 attcagcccg aagaaatagc cgaaattttc ttgcgacacg ccataaagcc caatatagac
     6901 aatcgagcct gaggttaaaa aacagaaaat acctgcaaag gttaagccgc caatcagcac
     6961 ataaccaaga gtaggcctat gtttcagtaa gatggcaaaa ttgcttaaag ttttgccaaa
     7021 acttaacggc aggcgttttt cgggggctaa tgtttcaggg attttccaac taattaatac
     7081 cacgcacaaa atccccatcg ccactaacac atagaaaatt gagtgccagt ggaaatattt
     7141 ggcaatataa ccgccaacaa tcggtgcaag caatggggca agcatagaaa taatcataat
     7201 aaccgacatc aatttggcaa ataaattacg ctcaaacaga tctctcacta atgctcccag
     7261 caccaccgcc ggggctgccg cacataagcc ctgtactaac cttagcaggt aaaatgtttc
     7321 aatattctga acttgggtca gaaaataagc ggcaatcgcc gagccgacta atccgattaa
     7381 aataatgatt ttccgcccaa agctgtctgc catcggtccc cagaatagtt gccctgcccc
     7441 aaaacctagg gtaaataccg ccaaggtggt ttgcactttt tcttgcgaaa ccgccaagtc
     7501 acgggcaatg tctaaaaatg agggcaaata catatcaatg gctaatggtg gcaacattgc
     7561 catcatacct aatatgatga aaaagctcag gcttggtctt ctactttcca ttaaaactgg
     7621 ctatctcctc tgaagtgagc gagcggaatt caccttcttc caacctttca tctaacacca
     7681 catcgccaat tcgccagcgg tgcaatgcct cgactttatt cccaagggcg gcaaacattc
     7741 gcttaacttg atgataacgc ccctcactaa tagttaaatt cacattgtaa tcgtctaaaa
     7801 tttcaagttg agccggcaag gttggctctt tctcgccacg caataaaatt ccttctgcca
     7861 atcgctgtgc ataaaaatct tccaccggat cagcaagcgt cactaagtat gtcttttcac
     7921 aatggtgctt gggcgaggta atacggtgcg accatttgcc gtcatcggtt aacagcacca
     7981 gccctgtggt atccacatct aaccgccctg cggtatgcaa tttagtcatt aacggataat
     8041 caaagaattg aaacaccgtt gggtactcac catcatcgtg tgaacaaata tagccctgtg
     8101 gcttatagag cataaaatac tgcccctctt ccacccactc taatttctga tcttcgtaat
     8161 aaatttcatc ttcttgactg atttttgccg caccactttt ttcaatattg ccgtttaccg
     8221 tcaccacacc acttttcagc actttagccg cttgtgaacg ggtcaaaccg gtattttcgg
     8281 cgataaattt gtctaaacgc atactatttt tataccataa attactttac cctctcccct
     8341 cgtgggagag ggacagattt ttcttcgtct agaagaaaaa tcagggagag gggggaaata
     8401 cgtcttcgtc cagaagaaaa atcagggaga ggggaaatgt gcaaactttt ttagaaaaat
     8461 gaccgcttcc cctctctctg cttatttcgc tgaacgctca taagcggtct ctctcccaca
     8521 aggggagaga gtgtttaatt gggagattat accacaactt ccctaacaaa ttcgctttct
     8581 agcccgacca acggcaagct agagtcgtgc caatctcgca ataattggtg ttgcattaaa
     8641 tttaacgtat ccaaccccgg cacactgttt ggggtgtatt gttgggcgat acgagccagt
     8701 tgggttaagc ccaaactgct ctcaagactt gaactaatca ccgctactaa tccttgcttc
     8761 tgtgcttgtt gaattaaatt cacgcatttt tcaagcgaac caaccagcgt aggtttaatg
     8821 ataatagcgg tcaaatttgg ctcttttttt accaaaaaat tcgcctctct cacggtttcg
     8881 tcccacgcaa tcgcaatgtc ggtttgtgtg gcaaattggc gggaaagttc gagcgcttgg
     8941 caaggttctt cgataaattg aatacggggt ttatgttcat ttgcaatttt ttcagcaaat
     9001 ttgaccgctt gttccaagct ccaaagacgg ttggcatcaa gccttaggcg taaattcgga
     9061 aaagtatgca acagcaaatt tgcctgctcg ccttcctgtt caggattgag accgatttta
     9121 attttaccga ttgggttcgt atgttggcta aatgccgtgt gtaacttttc caaatcgcca
     9181 tagcacagca ccgccgattg gttattcgct tgctcgccta attcattatt cagttcagcc
     9241 aacgcacagc ttagcccaaa ggcaacagac gggaaaagct gctctaacgt ttcaggctct
     9301 cctttctgcc acgcctttaa ccaattcaca gcttggtttt cagcttgttc taggctctct
     9361 tggctaaaac ttggaagcgg tgcaatttcg ccccagccga ctttctcctg ttcttgcaaa
     9421 cacaccaaca ccccttgcct ttctttcaaa ggttgcttgc gtaacactac accggtttga
     9481 atggggagtt ggtagcggta taactttact tgtcgcatac tatttttatc caaaataggt
     9541 ttagaggcga aacatctttc gcccacatca tcatttggga aattgaggcg aaaaatgttt
     9601 cgcctctagc gcatcatatt ctaccgcttg ttagcccaaa gctctaataa attcacgcca
     9661 actggtaaac ctgccacttt tgggctaaaa tagacggcaa tttttaccct aattttaatg
     9721 agaaaaagaa tggctaacca aaaacgaaaa atgcttgtta cttgtgcctt gccctatgct
     9781 aacggtgcaa ttcatttagg tcatatgctt gaacatattc aagcagatat ttgggtgcgt
     9841 tttcaacgta tgcgtggcaa tgaagtccac tttgtctgtg ctgatgatgc ccacggcacg
     9901 cctattatgc ttaatgccga taaattaggc attacccctg agcagttaat cgaaaaagcc
     9961 aaagccgatc acgaagcgga ttttgccagt tttaatatca gctttgataa ttaccattcc
    10021 acccacagcg aagagaaccg ccaaatcact accgatattt ataaaacctt aaaagcgaac
    10081 ggttttatta aaagcaaagt gatttcacaa ctgttcgacc cagaaaaaaa tatgttcttg
    10141 ccggatcgct ttgtgaaagg gacctgcccg aaatgtaaag ccgaagatca atatggcgat
    10201 aactgtgaag tatgtgcctc tacctatagc ccgatggatt taatcaaccc tcgctcggca
    10261 gtttccggtg caactccggt ggtaaaagaa tcagaacatt tcttttttga cctgccaagc
    10321 tttgagggaa tgttgaaaga gtggactcgt tccggctcac tccaacctga aattgccaat
    10381 aaaatgcaag aatggtttga aagtggttta cagcaatggg acatcagccg tgatgcccct
    10441 tatttcggtt ttgaaattcc ggacgcagaa aacaaattct tctacgtttg gttagatgcc
    10501 cctattggtt atatagcatc gtttaaaaat ttatgcgacc gtgcaggcat tgattttgat
    10561 gagttttggc aaaaagattc caccaccgag ctttatcact tcatcggtaa agacattgtt
    10621 tacttccaca gcctgttttg gccggcaatg ttggaaggca gcggctaccg caaaccgacc
    10681 aatgtgtttg ctcacggtta tgtgacagtg gacggggcga aaatgtcgaa atctcgtggt
    10741 acattcattc aagccaacac ctatttaaaa catctcgatc cggaatgctt acgctattac
    10801 tatgcggcaa aattaaacga tcgcattgaa gatttagatt ttaatttaga cgacttcgtg
    10861 caacgtgtaa acagcgatat cgtaaacaaa ttagtgaatc ttgcttctcg caacgccagt
    10921 tttatcgcta aacgctttga gggcaaatta gctgaaaaat tagatgacga agcgttattt
    10981 aatgaattta ccgctcaagc agaaaccata gcgaatttct atgaaaaccg tgaatttaat
    11041 aaagctattc gtgccattat ggaattaacc gataaagcca ataaatacat tgacgacaaa
    11101 gccccttggg tcatcgccaa agaagaaggc aaagaggctg aattacaagc ggtttgttca
    11161 atgggcattg agttattccg tgtgctgatg agctacctaa aaccggtgtt accaaaactg
    11221 gcagaacgtg ccgaagcatt cttacaaagc gaattgcgtt gggataatat cgctacccca
    11281 ttattcgggc ataccgtggc accgtttaaa tcgctattct cacgccttga gaaaaaacag
    11341 attgatgcag tgattgatga aaccaaagcc ttatttgccg aagcgaataa gaaaaccgaa
    11401 aaccaaacca aaggaacaca aaaagtggaa caacaagcgg tcgaaaaaca agaaaatatt
    11461 gcagttgagc caatcagccc tgaaattacg attgatgatt tcgcgaaatt agatttacgt
    11521 gcagccaaag tattgaactg cgaggctgtg ccaaaatcag acaaaatgtt acgttttgag
    11581 ctggatttag gcgaccacca acgccaagta ttctcaggca ttaaggcggc ctacccaaat
    11641 ccggaagcac ttatcgggcg ttttgtgatt gtaattgcca accttgctcc acgcaaaatg
    11701 tcgttcggta tgtcggaggg aatgatttta tctgccggca caggcggtgc gaacctgttc
    11761 ttattagatg cagacagcgg tgttcagccg ggaatgcaag ttaaatagga gaagatgatg
    11821 attcaacaaa cgctttctat tattaagccg gatgctacac gccgtaattt aatcggcaaa
    11881 attttggctc aattggaaga aaacgggctg actatcaaag ctctaaaaaa agtacatctt
    11941 acccgtgagc aagcggaagg attttatgcg gaacacaaag gcaaagattt ctttgagccg
    12001 ttagtggaat ttatgacatc aggatctatt gtcgtttctg tactggaagg ggaaaatgca
    12061 attagccatt atcgtgcatt aatgggggca accaatccgg ataaccgtga aaaaggcacc
    12121 attcgcgatt tatatgcctt aagtatgcgt gaaaattcgg tacacggttc agattccgaa
    12181 gagtcggcaa aacgtgaaat tgcctacttc tttgtaccga gtgagattgt ttaataaagt
    12241 aggggcgaaa attttttcgc cccactattg taattcgccc tagcgtagat taaatatatt
    12301 ctacgccaac aatgtcaaac tcaactttgc cacccggtgt ggtaatgctg actgaatcgt
    12361 ccacctcttt gccgattaaa ccacgagcaa tcggggaatt aactgaaatt aagccctctt
    12421 taatattggc ttcatcatca cccacaatgc ggtaagtgac ttcttcatct gtatcagcat
    12481 tcactaactg caccgttgcc ccaaaaatca ctttgccgtt gtttgccatt ttagtgacgt
    12541 caataatttg agcattgcct aatttgcctt caatttcttg aatacgcccc tcacagaaac
    12601 cttgttgttc acgggctgcg tgatactccg cgttctcttt taaatcaccg tgttcacggg
    12661 cttctgcaat cgcatcaata atttgtgggc gacgcacatt ttttaaaaaa tccaattctt
    12721 cacgcagttg ctctgcacca cgcaccgtca taggaatttg tttcattaaa tctatttcct
    12781 tactaaaaat tatcggttta cgaattaaaa cgaaagggct attattcaat ttctcactaa
    12841 aaatttcaac tcccctttta ttatgatttt taaaaatttc actcgtgctt ttttaaataa
    12901 atttatctta tctacacttc tgattccaag cattacacag gctgaaatta ataccgaaga
    12961 attattaaat accttgcctg ccggtacttc tgttagtttt attgctaaaa atttagacac
    13021 taaccaaatc atcacccaat accaaagtga tatttttatg ctacctgcca gtacccaaaa
    13081 agtctttacg gcactggcag caaaattaac tttaaacgat gatttccgct ttcaaactgc
    13141 ccttttaacc aacggaaaag tagaaaacgg agtgttaaaa ggcaatttaa ttgcccgttt
    13201 tagtggcgac cctgagctta caagcggtca aatttatcaa ttaatgagca aattaaagca
    13261 acaaggtatc aataaaattg agggggattt aattctagac ccgtcggttt tcgccagcca
    13321 cgacaaagcc tccggttgga tttggaacga cttgacgatg tgctttaacg ccccaccggc
    13381 ggcaattaat gtggatcaca actgttttta tgtcacctta aatgccgacc aacccattgg
    13441 cgaatttgct aaagtgaatg tgccgtccgc ttatccggtg caggtgttta gctctgctta
    13501 tattgtcgag ccgaaagaag ctccgttttg ccagcttgat gtggtggttc acgataacaa
    13561 ccgctaccaa atcaaaggct gtatggctcg ccagtctcaa ccgttcggct taagtttttc
    13621 ggtgcaagac ccaactaatt acggggcaaa tatgcttaaa gcccagttaa aaagcctgaa
    13681 aattgccttt aacgggcaag taaaagagcc acttactgcc caaaacggca cgctacttgc
    13741 cgagcattat tccgagccgt tgccggtatt attgaaaaaa atgatgaaaa aatcggataa
    13801 ccaaattgcc gatgccctgt ttagaaccgt tgccaacaag caacacaacc gccccgcatc
    13861 gttccaatta ggtagttatg tgattcgcca gttattaaaa accaaagcca atattgattt
    13921 taaaaacagc gtggtggctg atggctcagg gctttcccgt cataatcaag tcagcagccg
    13981 aacaatgctg gagacccttg aatatatcgc tcaaaacgaa gaaagcttaa aactgtttga
    14041 aaccttcccg attgcaggcg tggacggtac tatttccggc agaggctcta tttcaacaga
    14101 accgcttgcc aaaaatctga ttgccaaaac cggctcactg aaaggggttt acaaccttgc
    14161 cggctttatg aaaaacgccc gtggcgaacg catcgctttt gtgcaattta ttaacggcta
    14221 ttccaccggc gagctcgaaa gcaaaaccaa acgggctccg cttaatacat ttgaaaataa
    14281 gttttatatg gcgttgttta atgaataggg cttatatttt gtgtgatgga tttttgagaa
    14341 ttgaataaga cagcggtcgt aaatttttga aattttacga ccgcttgctc ctctctaagc
    14401 aattaaatct cgatgacttc gttgcatttt tacctcaata taactacaac ttggcttgat
    14461 tttcagccct ttttcttgcg caaacgcaat taaagcattg taaagctctc ctgcaatacc
    14521 tttaccttga aaggcatcat ctactcgggt actgtaagca tcaatcacgt tctcttttat
    14581 gtagcgataa cgtagtgtgc ctgcttttag gttgttttcg gtaaaatact caaatacgaa
    14641 atctgctaaa ttgtggttaa tcatagttcc tccgattttt ttcctctatt ctacccgctt
    14701 taatcacgag tgcaagcggt cgattttgca gaaaaatttg caaaatcgac cacttgtaga
    14761 tgaaaaaagc aaacgtttgc gttataatct ttgccgttta ttttcacgtt aacactcatt
    14821 ttaaggaatt taagaatggc gattcaacag gcgttattaa gtgtgtctga taaaaaaggc
    14881 attgtcgaat ttgctcgtgg gctatcggct cgtggggtaa aattactttc tacgggcgga
    14941 accgcaaaat tactggctga agccggtttg ctagtaacag aagtatcaga ttacaccggc
    15001 tttcctgaaa tgatggacgg gcgtgtaaaa accctgcacc cgaaagtgca cggcggaatt
    15061 ctaggtcgcc gtggtactga tgatgaagtg atgaaccaac acggcattga acgaattgat
    15121 atggtggtag taaacctcta cccttttgct caaaccgttg ccaatccaaa ttgtagcctt
    15181 gaagatgcgg tggaaaatat tgatatcggt ggtccgacaa tggtgcgttc tgcagcgaaa
    15241 aatcataaag atgtggcgat tgtggtcaat aacaatgatt ttgatgctat tcttgccgaa
    15301 atggatcaac atcaaaacag tttaacttta gaaacccgtt ttaatcttgc tattaaagcg
    15361 tttgagcata ccgcacaata cgattcaatg attgccaact atttcgggca aaaagtgccg
    15421 ccttatcaag gggctgagga agaagattta acccaaccaa gtggacaatt ccctcgcacc
    15481 ttaaacctca attttgtgcg taaacaaacg atgcgttatg gcgaaaattc gcatcaaaac
    15541 gctgcattct atgtcgaaaa tgagttaaaa gaagcctctg tttccaccgc caaacagtta
    15601 caaggtaaag ccctttcgta taataatatt gcagataccg atgcggctct tgaatgcgtg
    15661 aaagaattta gcgagcctgc ttgtgtgatt gtaaaacacg ctaacccttg tggtgttgct
    15721 ttgggtaaaa atattttaga tgcctacaac cgtgcttacc aaaccgatcc aacttctgct
    15781 ttcggtggca ttattgcctt taaccgtgaa ttagacggcg aaactgctca aaccatcgca
    15841 gaccgtcaat ttgttgaagt gattattgca ccaaccgttt ctgcttctgc caaagaagtg
    15901 ttagccacta agaaaaacgt gcgtgtgctt gaatgtggcg agttctcaga agctgtaaaa
    15961 cgcttagatt tcaaacgggt aaacggcgga ttattagtgc aagatgccga ccaaggctct
    16021 gtatctattg acgatttaaa ggtggtttca gagcgtaaac caactgaagc tgagctaaaa
    16081 gacctattgt tctgctggaa agtggcgaaa tatgtgaaat cgaatgcgat tgtgtatgcc
    16141 aaagacgggc aaactatcgg tatcggtgca gggcagatga gccgtgttta ttccgcaaaa
    16201 attgcaggta ttaaagcgga agacgagggc ttacaagttg tgggttgtgt aatggcttcc
    16261 gatgcgttct tcccgttccg tgatggtatt gacgctgctg caaaagtagg gattacttgt
    16321 gtgattcacc caggtggttc aatgcgtgat caagaagtga ttgatgccgc caacgaacac
    16381 ggtatggcag tggtactaac cggaatgagg catttccgtc attaatgttc tacaaactaa
    16441 aaggctacct tattttgata aggtagcctt tttcattaat taatcacaag gtaaaatttt
    16501 gattgctaag ccaccggttg aggtttcacg gtattttgca ttcatatctt tgccggtttc
    16561 gtacattgtt tcaatgactt tatctaaact tacctgaggc gaagaagtac ggcgtagtgc
    16621 cattctgctg gcattaatcg ctttgactga agcgattgca tttctttcaa tacaaggcac
    16681 ttgcacttgt ccgccaacag ggtcacaagt taggcctaaa ttgtgctcca ttgcaatttc
    16741 tgcagccata cacacctgtt cagcactgcc acccataatt tccgttaaac ctgctgccgc
    16801 cattgaacaa gcaacaccaa cttccccttg gcaacctact tcagcaccgg aaatagaggc
    16861 attcattttg tataacgaac caatcacact ggtagtcaat aaataacgct caatagtatc
    16921 ttgattaagc tgtgcaataa atttttggta gtacgccaac acagccggaa caataccaca
    16981 ggctccattg gtaggtgcgg tgactactcg cccacctgcc gcattttctt cattcacggc
    17041 aagggcaaac atattcaccc aatcgataat ttgcattggg tcggtattga atttactgtt
    17101 tgcctctaac attctgcgta gcccactggc acggcgtacc actttcagag gcccgggtag
    17161 taacccttcc gtattcaaac cacgctcaat acaggcttcc attgtattcc aaatcattgc
    17221 caaataatcc gaaatttcta ctctactgcg taaagcaagc tcatttttcc agactaaaga
    17281 agaaagcggt aacccgttat tcttacaatg tgcaagtaaa tctgctgcat tctgatatgg
    17341 gaatggaaca gaaagatctg aatttttaac ttgctcaaag ttagcttcat caacaataaa
    17401 gccaccacca atagaataat aggtgttttg aaatagcaac tcttcaccgc tcaaggcact
    17461 aaatttcaca ccattttcgt gccgaggtaa gaaatctcga tgaaatggca tatcctcata
    17521 aaaatcgaac gcaatcactt taggctcaac actgctttca gcaatgataa gttgcttatt
    17581 ttgttttaga ttttcaatcg tctgctcaat acgttcaata tccacattat gaggcaaata
    17641 ccccattaat cccattatga tggcaatatc cgtattatgt cctcgcccgg tgagtgataa
    17701 tgaaccatag acatctaccc gcaagcggtc aattttgttt aaaaatttgc gattttgtaa
    17761 ttcatcgaca aattcctttg ccgctttcat cggccccgca gtatgggaac tggaaggccc
    17821 aatccctact ttaaacatat caaatacact aatcataaac acaaattcac atagaaaata
    17881 aaagggcgta ttctacattg attttagaaa gattacagta acccatatac tattgctgaa
    17941 atagcaatac aacccataac aagcacaaat aaatggccaa attcaccttt aaaacgtgca
    18001 agtgccggta ctttatatac ggcatagaca ggcataataa acaagatcat cgcaataatc
    18061 ggcccaccaa ggctttcaat gaagcctaaa atgcttggat tgatagtcgc aacaatccat
    18121 aaaatgatca aaaaagtaat agcggtaatt ttgcggaatt tgcttggatc aatcggctct
    18181 ttacgcattt gattaaccaa tccttctaac ccctcgcgcg cacctaaata atgcccaaag
    18241 aaagagctac caatcgccaa aaaagcaacc aacggtccaa aataggaaat aatcggatta
    18301 tcaaatttat tcgcaaggta agaaagaatc gaaatatttt gcactttagc ttgtgctaat
    18361 tcttcaggtg tcaaagttaa tacacagcta aatacaaaga acatcacaaa taacaccaag
    18421 ataaaggcgg tagaacgtaa tactttagct gattcaattt cagccttctt gtcatcttgg
    18481 taatatttct gttgagaaag tgcaaaagaa gaaatagccg gtgaatggtt aaaagcaaag
    18541 actaaaacag gaatagtcag ccaaagtgta gtaaggaagt ctcctgtagt tggcatttgc
    18601 tgtaaggcag ctgtattcca ctgtggaatt aaataaatag ataagcccaa taaaatcaac
    18661 actaatggat acacaagaca agttgtgatt tttaacatga cttgctcact taataacatt
    18721 actccgatca tagcagctat tagcataaac gataatacag ccctagatgg tggcgcgata
    18781 tgaagctgat tctccataaa agaactcacg gtattggtaa tacctacacc ataaatcagt
    18841 aaaatcggaa aaatggcaaa gaagtacagt agcgtgatca atttaccggc ctgttcacca
    18901 aaatgttcac gaacaacagc cgtaatatcg ctccctttgt attttgaaga aagcacaaaa
    18961 cgtgttaagc cacggtgagc taaatatgtc attggtccaa ctaacaaagt cataataata
    19021 agtgggtaga acccacccat acctgcatta attggcaaaa ataaaacgcc ggctcctacc
    19081 gcagtaccaa ataaatttag tacccaagta agagaaaaac gttgagatgg catagccata
    19141 gtaatctcct ttaaagaatt atctaacaag ctatgctata ttagcgatgg gaaacagtaa
    19201 cacaacaaga ttttacgtat tttttgatca aactattaaa agcagaaaaa atagtgtttt
    19261 tggcgagtaa aaatccatca aacaaaatta aattttactt aaatcaaaga aatttagaat
    19321 tttattttta tgtggaaatt tcttttagat atgaaaaaac cggttagaat taacttaacc
    19381 ggtttcaaat ttgatttgca aaaaataact aaattaagcc cgcttgttgt aatgctttta
    19441 acactacagg ctgtgctgat tcagacagtg tggtaagcgg taaacgtaaa ttaggctcac
    19501 taattaaacc taatttataa gcagcccatt tcaccggaat cgggttagat tcgacaaata
    19561 agtcatggtg taacgccatt aagcggttat taatttcttc tgcttcagta aattgaccgg
    19621 caagagctaa ctcacacatt tttgccatat cagcagcagc aacattattg gtgaccgaga
    19681 tcaccccttg accacctaat ttcatagact caaaaccggt agcatcatca ccacttaaga
    19741 aaataaaatc ttcaccggct aattgcttaa ttaaaggtaa gcggcttaaa tcacccgttg
    19801 cttctttcac agcaacaata ttctcaattt tcgctaagcg accgattgtt tcaggtttta
    19861 aatcactccc tgttcgcccc ggcacattat acagaatttg tggtaaatct gtactttctg
    19921 caatcgcttt gtagtgtaag tacatcccct cttgcgttgg cttgttgtaa taaggcacta
    19981 cacttaaaca accagctaca ccactgtttg ctaataattt agtcatagtg atagcttcac
    20041 tggtcgcatt agaaccagtt cctgcaataa ttggaatacg tccatcagca aattcaaccg
    20101 ttttcttaat caccttaaca ttttcatcaa tgcttaatgt ggtagattca cctgttgtac
    20161 caacagatac aatagcgtga gaacctgctt gaatatgata ctcaactaat ttttttaaat
    20221 cctgataatc tacctcacca tgagtcattg gtgtaactaa tgctacgata ctaccgtgaa
    20281 ataaaggggt cgccataatc atctcctgta tttttttatt atgcttgata acattgctag
    20341 attgcaaaat tcagcttaaa tatgcaagca cttttttgaa attatttaaa attaatcata
    20401 taggctttga tcaccttcat ttgggcgagt tttaaaacgg cgatgtagcc acatatattg
    20461 agttagcgca gccataattt cacgttctac cacccgattc attaaaatag ccgtttcaac
    20521 ttcattttga cattgactaa aatcaacggc aggactgact tttactgtgt agcctgaata
    20581 gtccaaattt cgaatcgggc taaacgggat cactaaactc tttggggcag aacgaagtaa
    20641 catataacta cccgcagtag tacaagtttc ctccactgca aaaaatggta caaaaacgct
    20701 atttcgtctt ccataatcgt gatcaggcgc gtaccatatc atctcgccat tttttaaagc
    20761 tcgaatcatt ccacgcacat ctttacgatc taacatcgct ttatttgagc gaacacgtcc
    20821 tcgaaactgt atccaatcta ataatggatt atcattcggt cgataaaccc ctactccttg
    20881 atgatgtagc ccaataattc ttgcaccgag ttctaaagtc agaaaatgca cccctacaag
    20941 taaaataccc ctttcctgat cacgctgtgt taaatgctct agcccttcga ttttagacca
    21001 tttaagaata cgtttatccg accaaaacca agccatacca gtttcaataa tcgccattcc
    21061 aaccgatttc atattttctt cgactaattc ctctactttc tcagcagagt aatggggaaa
    21121 acagagctct aaattacggc gagcaatacg catacgacgt ttaccaaaac ccaatttagt
    21181 aaacaattta cctagtccca gaccaatttt tactaaaata ggataaggca aacataaaat
    21241 taacttaaaa acacctaaac cgagccacaa tccccaatat ttagggtgta aaaattgtgt
    21301 ctggaatgga ggtaactgtt ttttctcact cattaattat tctcacatac tagatta
//
LOCUS       DANLRK010000018        20946 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.018, whole genome shotgun sequence.
ACCESSION   DANLRK010000018 DANLRK010000000
VERSION     DANLRK010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 20946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20946
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.018"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(29..610)
                     /gene="dcd"
                     /locus_tag="PXA33_000533"
     CDS             complement(29..610)
                     /gene="dcd"
                     /locus_tag="PXA33_000533"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HDL5119897.1"
                     /translation="MRLCDTDIKRYLDEGIIAITPRPSDDKISGATADVRLGNSFRVF
                     REHKTPYIDLSGPREEVAAQLNKVMSDEIIIGDDEAFFLHPGDLALATTLESVSLPAN
                     IVGWLDGRSSLARLGLMVHVTAHRIDPGWEGKIVLEFFNAGKLPLALRPNMAIGALSF
                     EILSGDAAKPYNARKDAKYKNQQSAISSRINQD"
     gene            complement(625..1272)
                     /gene="udk"
                     /locus_tag="PXA33_000534"
     CDS             complement(625..1272)
                     /gene="udk"
                     /locus_tag="PXA33_000534"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HDL5119898.1"
                     /translation="MTDTSQTSSCVVIAIAGASASGKSLIASTIYKELKNELGTDNIG
                     IISEDAYYRDQSHLTMEERIKTNYDHPNSMDHHLLVEHLRQLKQGNAIEIPEYDYTEH
                     NRKTTTKHFEPKKIIILEGILLLTDENIRNEINVSIFVDAPLDICFIRRLQRDMVERG
                     RSMESVISQYRKTVRPMFLQFIEPSKQYADIIVPKGGKNRIAIDILKAQIKQLLK"
     gene            1376..2005
                     /locus_tag="PXA33_000535"
     CDS             1376..2005
                     /locus_tag="PXA33_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005620726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid transporter"
                     /protein_id="HDL5119899.1"
                     /translation="MDIFIQGFIVCFGLIVSIGAQNAFLLKQGILKQHIFWIALLCFL
                     GDVFLMTIGVLGLGTLIAELPVVSFIISLLGAIFLLSYGSRSFISVFKSGEYLVASGE
                     NATSLKKALLITFAITFLNPHVYIDTVVIVGSIGGKLDFNGKIYFLAGALLCSFIWFF
                     GIGYGASLLSPYFAKRRTWQILDAVTGLIMYFIAFSLLIYTYQLALQIF"
     gene            2120..3172
                     /locus_tag="PXA33_000536"
     CDS             2120..3172
                     /locus_tag="PXA33_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HDL5119900.1"
                     /translation="MFKKFLGLFSNDLSIDLGTANTLVYVKGQGIVLDEPSVVAVRQD
                     RMGSLKSIAAVGSHAKLMLGRTPKSIMAIRPMKDGVIADFFVTEKMLQHFIKQVHSNN
                     FMRPSPRVLVCVPAGATQVERRAIKESAIGAGAREVYLIEEPMAAAIGAGLPVHEAMG
                     SMVIDIGGGTTEVAVLSLNGIVYSTSVRIGGDKLDEAIIAYVRRHFGSAIGEATAERI
                     KQEIASAKIESDDDVKTMEVHGHNLAEGAPRTFELNSRHVLEAIEQPLNGIVEAVKSV
                     LEQCPPELAADIFERGMVLTGGGALLHNLDRLLSDSTGVQVVVAEDPLTCVARGGGKA
                     LEMIDMHGGDVFSIDD"
     gene            3274..4329
                     /gene="mreC"
                     /locus_tag="PXA33_000537"
     CDS             3274..4329
                     /gene="mreC"
                     /locus_tag="PXA33_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HDL5119901.1"
                     /translation="MKPIFAKAPSLGMRLIIAVIFSIMLIISDGRSSLMIQARNLLET
                     AVSGLYYFANTPRSILDGVSGNFIDNNKLQLENNLLKEQLREKSADLLLLDQLKVENQ
                     RLRLLLSSPLRQDEYKKIAEVLAAEMDAYRQQVVINQGRLDGAFVGQPIIDERGVVGQ
                     VISVGEKSSRVLLLSDITHAIPVQVLRNDVRGIASGTGVNNELVIDNLPRSIDVVKGD
                     VLVTSGLGGRFPEGYPVAIVETVENDTKSQFARIVARPLASFDRLRYLLLLWPTAEEK
                     NYSQSLSPAQVREVVEERRNSVNPFERLKQMSNKKTEVQLEAKEEQIENDEINENPAA
                     QPEAENEQNYHTEEGAE"
     gene            4329..4817
                     /gene="mreD"
                     /locus_tag="PXA33_000538"
     CDS             4329..4817
                     /gene="mreD"
                     /locus_tag="PXA33_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HDL5119902.1"
                     /translation="MRTNPIFQILVIIAIFVVAFVLEIMPWPVGFQGLRPTWVVLVLI
                     YWALALPDKMSVGTAFIAGIVWDLILGSILGIHALVLSVAIYFVAKYHLILRNLSLWL
                     QSLLVMAYIVLIRFFIFVIEYILHSAEFNSQELLGAVISGVLWSWIFLLMRHIRRQLR
                     LR"
     gene            4827..6164
                     /locus_tag="PXA33_000539"
     CDS             4827..6164
                     /locus_tag="PXA33_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HDL5119903.1"
                     /translation="MSSLSFDFSEDFRPLAAKMRPRNLAEYVGQSHLIGEGKPLRRAI
                     EAGHSHSMIFWGPPGTGKTTLAEIIAHHLDAEVERISAVTSGVKEIREVIEQAKLNRQ
                     AGRRTLLFVDEVHRFNKSQQDAFLPHIEDGTIIFIGATTENPSFELNNALLSRARIYI
                     LKPLQAVEILKILQMAIADTERGLGNETLVLEDDVLALLADYVNGDARFALNCLELMV
                     DMAQDSAKGKVLNKSLLTEVLGERQARFDKGGDRYYDLISALHKSIRGSSADGALYWY
                     ARILTAGGDPLYVARRLLAIASEDIGNADPRAMQIAIVAWDCFTRVGAYEGERAIAQA
                     IIYLAVAPKSNAVYQAFNEAKRLAKEAKDYDVPAHLRNAPTQLMKSLGYGEEYRYAHN
                     EPNAYAAGENYFPPELKDTEFYFPSERGMEKQIKEKMAWLKAQDQASPIQRYK"
     gene            6251..7036
                     /gene="znuB"
                     /locus_tag="PXA33_000540"
     CDS             6251..7036
                     /gene="znuB"
                     /locus_tag="PXA33_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005724066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HDL5119904.1"
                     /translation="MFDILFPAWLAGVLLAFITAPLGAFVVWRKMAYFGDTLSHSALL
                     GVALGIFLELDPYLAVIGMTILLALLLVWLEQKTNYSVDTVLGIIAHTSLSLGVITIS
                     LLDNVRVDLMSYLFGDLLAINFSDVVFIGIGATLICLILMLFWKRLLSITISPELAQI
                     EGLNVARLRLLLMILTALTIALSMKFVGALIITSLLIIPSATARRFAKTPEKMVLIAI
                     FFSIIAVSGGLLLSSWKDTPAGPSVVICAGLIFLLSLFKKEYH"
     gene            7161..7961
                     /locus_tag="PXA33_000541"
     CDS             7161..7961
                     /locus_tag="PXA33_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HDL5119905.1"
                     /translation="MCQLLGMNCNTPTDITFSFDGFRRRGGLTDHHTDGFGIAFFEGK
                     GVRVFRDNRPGASSPMAEAISQYQIKSLNVIAHIRKATRGDVNLENTHPFIRELWGEN
                     WVFAHNGTVEGVGVCEECHYQPIGTTDSEAAFCRIVSELKEMYPAKPSEKEIFDAVVQ
                     ITSRIAEHGVFNFVLSNGHWMIARCTTNLHYVCRKAPFGKALRDDDVSIDFSQYTKIT
                     DKVTIIATQPLTKNENWTKMKNGGYVFFKDGDVVYEIEGTLPEAQPHV"
     gene            complement(7996..8889)
                     /gene="ispA"
                     /locus_tag="PXA33_000542"
     CDS             complement(7996..8889)
                     /gene="ispA"
                     /locus_tag="PXA33_000542"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HDL5119906.1"
                     /translation="MSYLLATDLTHLQQRINHFLAEKLKTTDSVGSPLVEAMAYAVLL
                     GGKRVRPFLIYATGRMLGVPLEKLDHSAAAMEAIHAYSLVHDDLPAMDDDRLRRGKPT
                     CHIAFDEATAILAGDALQSFAFELLATDPHLTDREKVVQVTELATASGAKGMCLGQSL
                     DLIAENKAVSLEELELIHRNKTGALILSSVMMGLNLSEYSQNVAIKQPLVNYAKAIGL
                     AFQVQDDILDVIGETDKIGKIVGSDEHLNKSTYPKLLGLDGAKQKAEMLYQTALHSLE
                     QLPFDTTALKELANFIVKREN"
     gene            complement(8895..9125)
                     /gene="xseB"
                     /locus_tag="PXA33_000543"
     CDS             complement(8895..9125)
                     /gene="xseB"
                     /locus_tag="PXA33_000543"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HDL5119907.1"
                     /translation="MAKKPTQDFESTLKELEAIVTHLESGDLPLEEALNEFETAIKLV
                     QQGQERLQKAEQRIQILLNKNDTAPLSDYERE"
     gene            9250..10038
                     /locus_tag="PXA33_000544"
     CDS             9250..10038
                     /locus_tag="PXA33_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HDL5119908.1"
                     /translation="MQVTELNIYPIKSTQAIQVSQAFVEPKGLNFDREFMLAEPNGKF
                     ITARKDPELYRLAAFPIATGVMITHTSGQKCVALYQDFVEEQSSEVWGTHFNAKVAAE
                     AVNQWLSEIFGRAVQLRWLGSQSQRIVANFADHPMSFADSNPVSLMSLKSLEQVQQWS
                     PIPLEMARFRPNIVIDGNVAFEEEQWQQVQIGEVLFTKAALCTRCILITRDLNTLELD
                     PKSEPFRTLKEKHCDEKGKPVFGIHLVPQNSGVIKVGDPVIIQL"
     gene            10071..12020
                     /locus_tag="PXA33_000545"
     CDS             10071..12020
                     /locus_tag="PXA33_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HDL5119909.1"
                     /translation="MYQRSQSRTVLFPILLFVGINLVILTLSRLVLGVWQSDRVSAVD
                     GWIPLILQGIRIDISSLCWLFGLPALFSVLCFHKNVLGSIWQTVLRIWLTVGSVFILF
                     MEVATPNFIETFDLRPNRLFIEYLVHPREVFTMLINGHLWAVICGIGIAMISLIIYWK
                     LSGWATKNLHFPSWKMRPLLALLVAAIVFVGGRSSFAHRGINPAMVAFSSDPLVNSLV
                     LNSGYSVLYAVQQLKDEDKSSEQYGKMSVEEMFNIVKQVRNRPASDYISDEIPTLTQN
                     KATYQGKPKNIVIILEESLGAQFVGSLGGKNLTPNLDKLSEQGWYFTNLYATGTRSVR
                     GIEAVTAGFTPTPARSVVKLTKSQTNFFSIAELLKRQGYHTSFIYGGEKHFDNMASFF
                     YGNGFQQIIDEKDYKNPKFTATWGVSDEDLFDKANETFNQLHKSGKPFFSLVFSSSNH
                     DPFEFPDGKIELYEQPKQTRHNAAKYADYAIGHFFELAKKSEYWQDTVFLVIADHDSR
                     AVGEHLVPIQHFHIPALLLGEHIQPRTDNRLVSQLDMPTTLLSVAGISGQYPMIGYDL
                     TQDIDPNRAFMQYDATQAMMRGNDVVVMRPNLPIEGFSYDKANYKLTPKEQPESFKKE
                     ALAHALLGSYLYKNQLYKLPEETKQ"
     gene            complement(12091..13008)
                     /gene="nagK"
                     /locus_tag="PXA33_000546"
     CDS             complement(12091..13008)
                     /gene="nagK"
                     /locus_tag="PXA33_000546"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005708433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HDL5119910.1"
                     /translation="MAIYYGLDVGGTKIELAAFNEKLEKLHSERVPTPQTNYQDWLTA
                     IKTLVETADTKFDEKGTVGLGIPGFVNRETGIAEITNIRVADGKTILKDLAECLGREV
                     RAENDANCFALSEAWDESNKPYSTVLGLIIGTGFGGGFVFDGKIHSGRIGMAGEVGHM
                     QLNYHALKLLDWDKAPIYECGCGNQACLDTYISGRGFELLFNDLVGEKISAKAIIERF
                     YQQETKVVAFVEKYIELMAISISNLITILDPDMIVFGGGLSNFDYIYQALPKALPKHL
                     MRSAKVPVIKKAIHGDSGGTRGAAALFLK"
     gene            complement(13021..13383)
                     /locus_tag="PXA33_000547"
     CDS             complement(13021..13383)
                     /locus_tag="PXA33_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5119911.1"
                     /translation="MYQVFIAEPKDPNLEKGLLKHFIKSYLTQEELDFLHKILTNLPN
                     IRPFWGDTGYKYIVIDNVYFLYEVSESETKVILLGTKFNNKRKTFNFANTKTQQKSMK
                     KWINDLAIYKPHKINQSQ"
     gene            complement(13391..13552)
                     /locus_tag="PXA33_000548"
     CDS             complement(13391..13552)
                     /locus_tag="PXA33_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5119912.1"
                     /translation="MEMSIEKIAELLSDKEKDDYPFSIEERQFAAQASADTWEAILNN
                     TIQPFDFKK"
     gene            complement(13621..14343)
                     /locus_tag="PXA33_000549"
     CDS             complement(13621..14343)
                     /locus_tag="PXA33_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HDL5119913.1"
                     /translation="MFKKFSLYTLAMLLIPVVTWLANWHWSLAIQYAEFDFDYFLYLI
                     TETGSVPYAIITCVVFALLLARVVRKRYSLIFVITVCVASMVATQAIKTAMKSTFQEP
                     RPFVTALFQDKTDDFYQLTREQRGLAVMEKIGANQNFVTAHQAKEVGYSFPSGHTIFA
                     VSWLLLVVGFCRNMRGQAVVLAHFFAISWAALMLISRLRLGMHYPIDLFTSSLIAFLI
                     NLALFVWFVPWLESKKWFAKKP"
     gene            complement(14404..14718)
                     /gene="yhbY"
                     /locus_tag="PXA33_000550"
     CDS             complement(14404..14718)
                     /gene="yhbY"
                     /locus_tag="PXA33_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010786380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HDL5119914.1"
                     /translation="MSITLTTKQKQFLKGLAHHLSPVVMLGGNGLTEGVLAEIDNALD
                     HHELIKVKVAGADRETKQLIIDAILRETKAAEVQTIGHILVLYRQSEEKKISLPKATK
                     AI"
     gene            14845..15603
                     /locus_tag="PXA33_000551"
     CDS             14845..15603
                     /locus_tag="PXA33_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL5119915.1"
                     /translation="MPTLSVAMIVKNEAQDLRACLDTVKDWVDEIVILDSGSTDETPQ
                     IAAEYNARFYTNTDWQGFGKQRQLAQQYVTSDYVLWLDADERVTAELKQSILQAVKND
                     EKNTVYKISRLSEVFGKQIRHSGWYPDCVVRLYRTEFAKYGDELVHEKVHYPKEAKIQ
                     KLQGDLLHFTYKDIHHYLVKSASYAKAWAIQRANAGKTASLFDGVTHAIGCFVKMYLL
                     KAGFLDGKQGFLLAVLSAHSTFVKYADLWNRTRK"
     gene            complement(15668..16708)
                     /locus_tag="PXA33_000552"
     CDS             complement(15668..16708)
                     /locus_tag="PXA33_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HDL5119916.1"
                     /translation="MKKLLVIRNDKIGDFMVCFPAFAMLKQSMPNVEITALVPSYTAP
                     LAELCPSIDKIIIDTANKKDKNEFNRILQAVKNEQFDAVICFVSDWHNAKLTWQSGIK
                     YRLAPATKLFQFLYNHRLTQRRSQSAKAESEYNLDLARAFLSKHNIPIVEPHTPYLSV
                     EQQTVQAQKAKLCEQLNITTVKKWVFVHSSTGGSANNLSIEQYAELIQGILAEFDCYV
                     VLTAGKGESEKAQQLAQQINHNNVVVYDKNEGLQDFTHSLACADLFIAGSTGPLHISG
                     ALNIPTIGFYPSRLSAIPRRWKPINDANKHIAFCPPSDKASQMNLTLISISEALKTIV
                     PFIRRMWNTPSL"
     gene            16892..18223
                     /gene="argG"
                     /locus_tag="PXA33_000553"
     CDS             16892..18223
                     /gene="argG"
                     /locus_tag="PXA33_000553"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005722481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HDL5119917.1"
                     /translation="MSATILEALPLGQKVGIAFSGGLDTSAALLWMRKKGAVPYAYTA
                     NLGQPDEDDYNAIPKKAMEYGAENARLIDCRQQLAHEGIAAIQCGAFHISTGGIPYFN
                     TTPLGRAVTGTMLVAAMKEDDVNIWGDGSTFKGNDIERFYRYGLLTNPKLKIYKPWLD
                     QLFIDELGGRLEMSKFLIENGFDYKMSVEKAYSTDSNMLGATHEAKDLEELSTGMKIV
                     KPIMGVAFWDESVEIKAETVSVTFEEGVPVALNGKRIDDAVELIMEANRIGGRHGLGM
                     SDQIENRIIEAKSRGIYEAPGMALLHIAYERLVTGIHNEDTIEQYRINGLRLGRLLYQ
                     GRWFDPQALMLRETAQRWVAKAITGTVTLELRRGNDFTILNTESPNLTYEAERLSMEK
                     VEDAPFTPADRIGQLTMRNLDIVDTRGKLAVYTDAGLLAANNSVIPQLGKK"
     gene            18316..18777
                     /gene="rnhA"
                     /locus_tag="PXA33_000554"
     CDS             18316..18777
                     /gene="rnhA"
                     /locus_tag="PXA33_000554"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HDL5119918.1"
                     /translation="MKLVEIFTDGSCLGNPGKGGIGILLRYNGYEKTVSKGYFQTTNN
                     RMELRAVIEALAMLKEPCKVQLNSDSQYMKNGIQKWIFNWKKNDWKTSDKKPVKNKDL
                     WVALDQEIQRHQIEWSWVKGHSGHRENEICDELAKQGANNPTLDDVGYIAD"
     gene            18923..19426
                     /locus_tag="PXA33_000555"
     CDS             18923..19426
                     /locus_tag="PXA33_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HDL5119919.1"
                     /translation="MVDLIVIGIIAFSVLISLWRGFTNEALSLLGWVIAFFVASSFYP
                     YLSGYLTQINSVYLQNSEFLRNGIAAAILFILTLIVCGVINGLLGKVIDLSGMSITDR
                     ILGGAFGALRGILIVSAILFFLDTFTQASQTELWKSSLLIPHFDFIVKWFFEQLQANS
                     SFLNTTK"
     gene            19441..>20946
                     /gene="purF"
                     /locus_tag="PXA33_000556"
     CDS             19441..>20946
                     /gene="purF"
                     /locus_tag="PXA33_000556"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005647425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HDL5119920.1"
                     /translation="MCGIVGIIGNSPVNQAIYDGLTLLQHRGQDAAGIVTIDDENRFR
                     LRKANGLVSDVFQQEHMVRLQGNVGIGHVRYPTAGSSSVSEAQPFYVNSPFGITLVHN
                     GNLTNNAELKARLYNEARRHVNTNSDSESLLNIFAYFLDLYSTQHLSPDNIFETVRKT
                     NDSIRGAYACIAMIIGHGMVAFRDPFGIRPLVLGKREIEGKTEYMFASESVALDVVGF
                     EFVRDVLPGEAIYVTFDGQLHSQICADNPKLNPCIFEYVYFARPDSVIDGVSVYSARV
                     HMGELLGEKIKREWGRIIDDIDVVIPIPETSNDIAVRIANMLYKPYRQGFVKNRYVAR
                     TFIMPGQAQRKSSVRRKLNAIASEFKGKSVLLVDDSIVRGTTSEQIVEMARAAGAKRV
                     YFASAAPEIRYPNVYGIDMPTCEELVAYDRSVEEVAQMIGVDKLIFQDLEALYKSIQL
                     ENPTIHRFDDSVFTGEYITGDVDKCYLDSIARSRNDKAKAEAAKQATNLEIH"
BASE COUNT         6009 a         3859 c         4560 g         6518 t
ORIGIN      
        1 aatgtatgat tgcaaggcat gtactatatt aatcttgatt aatccgactt gaaatggcac
       61 tttgctggtt cttatatttt gcatcttttc gcgcattata gggttttgca gcatcaccac
      121 ttaaaatctc aaagcttaat gcgccaattg ccatattagg gcgtaaagct aggggtaatt
      181 ttccagcatt aaaaaattcc aatacaattt tgccttccca acccggatcg attctgtggg
      241 cggtaacgtg taccattaag cctaatctag caagtgacga acgaccatct aaccatccaa
      301 cgatattggc tggtaaggaa accgattcaa gggttgttgc gagtgcaagg tcaccgggat
      361 gaaggaaaaa tgcttcatca tcaccgatga taatttcatc actcatcact ttatttaact
      421 gagcggcaac ttcttctcta gggccactta aatcaatata gggcgtttta tgttcacgaa
      481 atacacgaaa cgaattacct aaacgcacat ctgcagtggc gccagaaatt ttatcgtctg
      541 aaggtcgagg cgttatggca ataatacctt catctaagta acgtttaata tctgtatcac
      601 ataaacgcat atttagctcc tattttattt tagtaattgc ttaatttgtg ctttaagaat
      661 atcaatggca atacggtttt tgccaccttt tggcacaata atatcagcat attgtttaga
      721 gggttcgata aattgtaaga acattgggcg aacggttttg cgatattgac taatcactga
      781 ttccatcgaa cgtccacgtt ccaccatatc acgttgtaag cggcggataa agcagatgtc
      841 cagaggagca tctacgaaaa tagaaacatt aatttcattg cgaatattct catctgtcag
      901 caataaaata ccttctaaaa taatgatttt ctttggttca aagtgcttcg tggtcgtttt
      961 acgattatgt tcggtatagt catattctgg aatttcaatc gcattacctt gttttaattg
     1021 gcgtaaatgt tcaaccaata aatgatgatc cattgaatta gggtgatcgt aattggtttt
     1081 aatccgttct tccattgtta gatgagattg atctctataa taagcatctt cggaaataat
     1141 accgatatta tctgttccca attcattttt gagttcttta tagatggttg aggcaatcag
     1201 gctcttacct gaggcagatg cgccggcaat cgcaatgaca acgcaagatg aagtctggct
     1261 agtatcagtc ataaaaaatt tcctattcag tcaaaagagt attcgataaa ttataccgaa
     1321 gttttgcaaa attttttgta taattgaccg cttgttatgc gattttggag aaaaaatgga
     1381 tattttcatt caaggattta tagtgtgttt tggcttaatt gtctctattg gcgcacaaaa
     1441 tgccttttta ttaaagcaag ggattttaaa acaacacata ttttggattg cattgctctg
     1501 ttttttaggt gatgtatttt taatgacgat aggtgtgcta ggcttaggca cgttaattgc
     1561 ggaattgcct gttgtaagtt tcattatttc gcttttaggg gccatttttc tattaagcta
     1621 cggtagccgt tcatttatca gcgtatttaa aagtggtgaa taccttgtgg caagtggaga
     1681 gaatgcaacg agcttaaaaa aggctttact gattaccttt gcgattacat tcttaaatcc
     1741 tcacgtttat attgatacgg ttgtgattgt aggcagtatc ggtgggaaat tagattttaa
     1801 cggtaaaata tactttttag caggtgcatt actttgctca tttatttggt tttttggcat
     1861 aggttatgga gcaagcttac tttcccctta ttttgcaaag cgaagaactt ggcaaatttt
     1921 agatgctgtt accgggctaa ttatgtactt tatcgctttt agtttactga tttatactta
     1981 tcaacttgct ctgcaaattt tctagtggag tttaaatttt ttcattgatg aatgacgggc
     2041 ttttttgcta tattagtagc aatgttcgca ctataaaatg tgcttatttt tgaataattt
     2101 tattttaacg gattctttta tgtttaaaaa atttttagga ttattttcaa acgatctttc
     2161 tatcgatctc gggacagcaa atacactggt ttatgttaaa ggtcaaggta ttgtgctaga
     2221 tgaaccctca gtggttgctg tacgtcaaga tcgtatggga tcgctaaaaa gtattgcggc
     2281 ggtaggctca catgcaaaat taatgctagg ccgtacacca aaaagcataa tggcgattcg
     2341 tccaatgaaa gatggtgtca ttgcggattt ttttgtaaca gaaaaaatgt tgcaacactt
     2401 tattaaacaa gtacatagta ataactttat gcgcccaagc cctcgtgtat tagtgtgcgt
     2461 gccagcaggt gcaacacaag tggaacgccg tgcaattaaa gaatctgcaa ttggagcagg
     2521 agcgcgtgaa gtctatttaa ttgaagagcc aatggcggct gcaatcggtg caggtttgcc
     2581 ggttcacgaa gcaatggggt caatggtaat tgacattggt ggcggtacaa ccgaagtggc
     2641 agtgctttct ttaaacggca ttgtgtattc aacttctgtg cgtattggtg gtgataagtt
     2701 agatgaagct attattgctt atgtacgtcg tcattttggt tctgctattg gtgaagcaac
     2761 ggcagagcga attaaacaag aaattgctag tgctaagatt gaatccgatg atgatgttaa
     2821 aacaatggaa gtacacggac acaatttagc tgaaggcgct cctcgtactt tcgagcttaa
     2881 ctcaagacat gtattagaag caattgaaca accattaaat ggtattgtag aggcagttaa
     2941 atcagtctta gagcaatgcc cacctgaatt ggcagcagac atttttgagc gcggtatggt
     3001 gttaacagga ggtggtgcat tattacataa tttagatcgt ttactttctg attcaactgg
     3061 cgtgcaagtc gtagtagcag aagatccatt aacttgtgta gctcgtggtg gtggtaaagc
     3121 attagaaatg atcgatatgc acggtggtga tgtatttagt attgatgatt aatacatttc
     3181 tcttagcagc tgtttaaatg gaggttggca atggattgat tctattgtca attttcgtta
     3241 tagattccca ataaattcac atatatttct ctaatgaaac ctatttttgc taaagctccc
     3301 tctttaggga tgcgcctgat tatcgcagtg atattttcta ttatgctgat tatttccgat
     3361 gggcgtagct ctttaatgat tcaagcgcgt aatctattag aaaccgcggt aagtggatta
     3421 tattattttg ctaatacccc ccgttcaatt ttagatgggg taagtggcaa ttttattgat
     3481 aataataaat tacagcttga gaataattta ttaaaagagc agctaagaga aaaaagtgcc
     3541 gatttattat tgcttgatca gctaaaggtt gaaaatcagc gtttaagatt gttattgagt
     3601 tctccgcttc ggcaagatga gtataaaaaa attgctgaag tattagctgc tgagatggat
     3661 gcttatcgtc agcaagtagt gattaatcaa gggcgtttag acggtgcttt tgtcgggcaa
     3721 cctattattg atgaaagagg tgttgttgga caagttattt ctgtagggga aaaatctagc
     3781 cgtgttttat tgctctctga tattactcat gctattcctg ttcaagtgct acgcaatgat
     3841 gtacgtggca ttgccagtgg tacaggggtg aataatgaat tggtcattga taacttgcct
     3901 cgttctattg atgtggtaaa aggtgatgtg ctggtgactt caggcttagg tggacgtttc
     3961 cctgagggat atccggtggc gattgtagaa acggttgaaa atgataccaa aagtcaattt
     4021 gccagaattg ttgctcgtcc gttagcctca tttgatcgct tacgttactt attactgctt
     4081 tggccgacgg cggaagagaa aaactactcc caaagcttgt cgccagcgca agtacgtgaa
     4141 gttgttgaag aaagacgtaa tagcgtgaat ccatttgaac gtttaaaaca gatgtctaac
     4201 aagaaaactg aagtgcagct agaggcaaaa gaagaacaaa tagagaatga tgagataaat
     4261 gagaatccgg cagcacaacc agaagcggaa aatgaacaaa actatcatac ggaagaagga
     4321 gcggaataat gagaacaaac ccgatttttc agatccttgt gataattgcg atttttgtgg
     4381 ttgcttttgt acttgagatt atgccttggc cggtaggatt ccaagggcta cgccctactt
     4441 gggtcgtttt ggtgttaatt tattgggcat tggcattacc tgataaaatg agtgtgggga
     4501 cggcttttat tgccggcatc gtctgggatc tgattcttgg ctcgatttta ggcatccacg
     4561 ccttagtgtt atcggtggcg atttattttg ttgcgaaata ccatctgatt ctgcgaaact
     4621 tatcgctatg gctacaaagc ctattagtaa tggcatatat tgtgctgatc cgtttcttta
     4681 ttttcgtgat tgaatacatt ttacacagtg ccgaatttaa ttcacaggaa ttgttaggtg
     4741 ctgtaatcag tggtgtgctt tggtcttgga ttttcttatt aatgcgtcat attcgtcgcc
     4801 aactgcggtt acgttaagag gttaaaatga gtagtttgtc ttttgatttt agtgaagatt
     4861 ttcgaccgct tgcagccaaa atgcgacctc gaaacttggc tgaatatgtc gggcaatctc
     4921 atttaattgg cgaaggcaaa ccgttacgca gagcgattga agccggacat agtcattcta
     4981 tgattttttg gggaccgccc ggcaccggaa aaacgaccct agctgaaatt atcgcccatc
     5041 atcttgatgc ggaagttgaa cgtatttccg ctgtgaccag cggtgtaaaa gagatccgag
     5101 aggtgattga acaagccaaa ttaaaccgcc aagccggcag acgaacgttg ctttttgtgg
     5161 atgaagttca ccgctttaac aaaagccagc aagatgcgtt tttaccgcat attgaggacg
     5221 gcactattat ttttattggt gcgactaccg aaaatccctc ttttgagcta aataacgcac
     5281 tgctttcccg agccagaatt tatattttaa agccgttaca agcggtcgaa attctaaaaa
     5341 ttttgcaaat ggcgatagcc gataccgaac gtggtttagg caatgaaact ttggtacttg
     5401 aagatgatgt actggcattg cttgctgatt atgttaatgg tgatgcccgt tttgccttga
     5461 actgtttgga attaatggta gatatggcac aagattcagc taaagggaaa gtgctgaata
     5521 aaagtttgct gaccgaagtg ttaggcgaac gacaagctcg ttttgataaa ggcggtgatc
     5581 gttactatga tctcatttct gcgttacata aatccattcg aggctcatcg gctgatggag
     5641 cattatactg gtatgcccga attttaaccg ccggtggtga tccgctttat gtggcaagaa
     5701 ggctgcttgc gattgcttct gaagatattg gtaatgccga ccctcgagca atgcaaattg
     5761 cgattgtggc ttgggattgc tttacccgag tgggggctta tgaaggtgaa agagcgattg
     5821 cccaagcgat catttatttg gcagttgccc caaagagcaa tgcggtctat caagctttta
     5881 atgaagcaaa gcgcctcgct aaagaggcaa aagactacga tgtgccggcg catttgcgta
     5941 atgctcccac tcaattaatg aaatctttag gctatggcga agagtatcgc tatgcccaca
     6001 atgagccaaa cgcttatgcc gccggggaga attatttccc gcctgagctg aaagataccg
     6061 aattctactt cccaagtgag cggggaatgg aaaaacagat taaagaaaaa atggcgtggc
     6121 tgaaagcaca ggatcaagcc agcccgattc aacgttataa ataaatatga caagcggtct
     6181 aaatcagcaa aaaatttgca aatttttccg aaaaaataac cgcttgtttt acttgccttt
     6241 gagaaccaat atgtttgata ttttatttcc ggcttggctt gccggtgttt tgttagcctt
     6301 tattaccgca cctctagggg cttttgtggt gtggcgaaaa atggcgtatt tcggcgacac
     6361 actttcgcac tctgcattgc tcggcgtggc attagggatt tttttagaac tcgatccgta
     6421 tcttgctgta attggaatga ctattcttct tgcattattg ctggtttggt tggagcaaaa
     6481 aaccaattat tcggtggata ccgtgttggg gattatcgcc catactagcc tttctctcgg
     6541 ggtaattacc attagtttgt tggataatgt acgagtggat ttgatgtctt acttattcgg
     6601 cgatttactg gcaattaatt tttctgatgt ggtgtttatc ggaatcggag caaccctaat
     6661 ttgtttgatt ttaatgctgt tttggaagcg gttgttgtcg attaccatca gcccggaact
     6721 tgctcaaatt gaaggattaa atgtggctcg cttacgttta ttgctgatga ttttaaccgc
     6781 attaactatc gcattaagta tgaaatttgt cggtgcttta attattacct cactgctgat
     6841 tattccgtct gctacggcaa gacgctttgc taaaacgcct gaaaaaatgg tgttgattgc
     6901 gattttcttc agtattattg cagtcagtgg cggtttattg ttatccagtt ggaaagatac
     6961 accagcaggg ccttcggtag tcatttgtgc cggcttgatt tttctattat cgctattcaa
     7021 aaaagaatac cattaaacag cataatgtat gaaaataaag cataagattt ttatgtaaag
     7081 agcattgaat tttctaatca atctgtttaa atgtaacaat tattttttgt tgtaaattat
     7141 ttggttttaa gaagcaaatt atgtgtcaat tacttggaat gaattgtaat acgccaaccg
     7201 atattacttt ttcttttgat ggatttcgcc gtcggggtgg gctgacggat caccatactg
     7261 atgggtttgg aattgccttt tttgaaggca aaggggttcg ggtttttcgt gataaccgcc
     7321 cgggagcatc atcgccgatg gcagaagcaa tcagccaata tcaaattaaa tcgctcaatg
     7381 ttattgcaca tattcgtaaa gctacgcgtg gtgatgtgaa tttggaaaat acccacccct
     7441 tcattcgtga attatggggc gagaactggg tgtttgccca taacggcaca gtggaaggtg
     7501 ttggggtatg cgaggagtgc cactaccagc cgattggtac aaccgattcc gaagcggcat
     7561 tttgccgtat tgtgtcggaa ttaaaagaga tgtatccggc taaaccaagc gaaaaagaga
     7621 tttttgatgc cgtggtgcaa atcacctcaa gaattgcgga acacggggta tttaattttg
     7681 ttttatccaa cggtcattgg atgattgccc gttgtacaac taatctgcat tacgtttgcc
     7741 gtaaagcacc gtttggcaaa gccttgcgtg atgatgatgt gtcgattgat tttagtcaat
     7801 ataccaaaat taccgataaa gtgacgatta ttgccactca gccacttacc aaaaatgaaa
     7861 actggaccaa aatgaaaaac ggtggctatg ttttctttaa agacggcgat gtggtatatg
     7921 aaattgaagg cacattgccg gaagctcagc ctcacgttta atcactaaaa aacctagctt
     7981 cggctaggtt ttttcttagt tttcacgctt aacaataaaa ttagctaact cttttaatgc
     8041 ggtggtatcg aaaggcaact gttccagcga atggagagcg gtttgataaa gcatttcggc
     8101 tttttgtttt gccccgtcta agcccaacaa tttcgggtat gtgcttttat ttaagtgttc
     8161 atctgaaccc actattttgc cgattttatc tgtttcgcca atgacatcta aaatatcatc
     8221 ttgcacctga aaggctaagc caatcgcttt ggcatagttt acaagcggtt gttttatggc
     8281 tacattttgc gaatattcgg aaaggtttaa tcccatcatc acggatgata gaatcaatgc
     8341 cccggttttg ttgcgatgaa ttaattcaag ctcttccaag ctcaccgctt tattttccgc
     8401 aatcaaatcc aaactttgcc ctaagcacat tccttttgca ccggaagcgg ttgccaattc
     8461 tgtaacttga acaacttttt ctcggtcggt caaatgcgga tcggttgcca gcagttcaaa
     8521 cgcaaaactt tgtaacgcat caccggctaa aatagcggta gcttcatcaa aggcaatatg
     8581 gcaggttggc ttgccacggc gtaatctatc atcgtccatt gccggtaaat catcgtgaac
     8641 cagcgagtaa gcgtgaatag cctccattgc cgccgcacta tgatctaatt tttccaacgg
     8701 cacaccaagc attctgccgg tggcatagat taaaaacggt cgcacccgtt taccgcctag
     8761 caataccgca taagccattg cttctactaa aggcgaacca acgctatccg ttgtttttaa
     8821 tttttcagcg agaaaatggt taatccgttg ttgcaggtga gtcagatcgg ttgctaatag
     8881 atagctcatt gtgattattc tctttcgtaa tcgctaagtg gggcagtatc gtttttattt
     8941 aataagattt gaatacgttg ctcggctttt tgtaaccgtt cttgcccttg ttgcactaat
     9001 ttgattgcag tttcaaattc gttgagggct tcttcaagcg gtaaatcacc ggattccaaa
     9061 tgggtaacga ttgcttctaa ttctttaagt gtgctttcaa aatcttgagt gggtttcttt
     9121 gccattgttg ctgacctatt gctgtaaaag ttgctcaagt ttaccgttct cacaagggat
     9181 ttgcaaattc cttttacttt tcgaccgctt gcaggtattg ttatcctatt tccaaatgga
     9241 ggattttcta tgcaagttac cgagctcaat atttacccga taaaatcaac tcaagctatt
     9301 caagtttcac aggcatttgt tgaaccgaaa ggcttaaatt ttgaccgtga atttatgcta
     9361 gccgagccta acggcaaatt tattactgcc cgtaaagatc cggaattata ccgtctagcg
     9421 gctttcccga ttgcgacagg ggtaatgatt acccatacaa gcggtcaaaa atgcgttgca
     9481 ctttaccaag attttgtgga agagcagagt agtgaagtgt ggggaacaca ttttaatgcc
     9541 aaagtggcgg cagaagcggt caatcaatgg ctgagtgaaa tttttggtag ggctgtgcaa
     9601 ctacgttggc ttggctcgca aagccaacga attgttgcga attttgccga tcacccaatg
     9661 agttttgcag atagcaaccc ggtgagtttg atgtctctaa aatcgcttga gcaagtgcaa
     9721 caatggtcgc cgattccact cgaaatggct cgttttcgcc cgaatattgt gattgatggc
     9781 aatgtggcat ttgaagaaga gcagtggcaa caagttcaaa ttggcgaagt gctatttacc
     9841 aaagcggcat tatgtactcg ttgcattttg attacacggg atttaaacac gcttgagtta
     9901 gaccccaaat cggagccgtt tagaacctta aaagaaaagc attgcgatga aaaaggtaag
     9961 ccggtgttcg gtattcacct tgtgccacaa aacagtggtg tgataaaagt aggcgatcct
    10021 gtcattattc aattataata tgccgatatt tttttaaaag gagattcaaa atgtaccaac
    10081 gcagtcaatc aagaactgtg ctatttccaa tcctactatt tgttgggatt aatttagtta
    10141 tcctcacctt atcccgttta gtgttaggcg tttggcaatc tgatcgagta tccgctgttg
    10201 atggatggat accattaata ctacaaggta ttcgcattga tatatcttcg ctatgttggc
    10261 tatttggctt accggcatta tttagcgtgt tgtgttttca taaaaatgta ctgggttcga
    10321 tttggcagac tgttttaaga atttggctaa ctgtcggtag tgtatttatt ctttttatgg
    10381 aagtcgccac accgaatttt attgaaacct ttgaccttcg cccaaatcgt cttttcattg
    10441 agtatttggt ccatccgaga gaagtgttca caatgctgat taacggacat ttatgggctg
    10501 ttatttgtgg gattggcatt gcgatgattt ctctcatcat ttattggaaa ttatcgggtt
    10561 gggcaaccaa aaatttgcat ttcccgagct ggaaaatgcg tccgctactg gctttattgg
    10621 ttgctgcaat agtttttgtt ggtgggcgtt ccagttttgc ccatcgaggg attaatccgg
    10681 caatggtggc attttcaagc gatccgcttg tcaattcact ggtgctaaat tcaggttatt
    10741 ccgtgcttta tgcggttcag caattgaaag atgaagataa atcttccgaa cagtatggaa
    10801 aaatgtctgt ggaggaaatg ttcaacattg ttaagcaggt tcgtaatcga ccagcttcag
    10861 attatatttc agatgaaatt cccaccctca cgcaaaataa agcgacttat caaggaaagc
    10921 ctaaaaacat tgtgattatc ttagaagaaa gtttaggggc tcaatttgtc ggttcgctag
    10981 gagggaaaaa tctcactcca aatttagata aactttcaga acaaggctgg tattttacca
    11041 atctttatgc aacaggtact cgttcggtga gaggaattga agcggtaacg gctggtttta
    11101 caccaacacc ggcaagatct gtggtaaaac tgacgaaaag ccaaactaac ttttttagta
    11161 ttgccgaatt attaaaacgt caaggatacc atacttcctt tatttatggt ggggagaagc
    11221 attttgataa tatggcgagc tttttctacg gtaatggttt ccagcaaatt atcgatgaaa
    11281 aagattataa aaaccctaaa tttaccgcaa cttggggcgt gagtgatgaa gatctgtttg
    11341 ataaggcaaa tgaaactttt aatcaactgc acaaatcagg taaaccgttt tttagtttgg
    11401 tattcagttc cagtaaccac gatccgtttg aatttccgga cggcaaaatt gaactctatg
    11461 aacaaccgaa gcagactcgc cataatgcgg caaaatatgc cgattatgct atcgggcatt
    11521 tctttgagtt agcgaaaaaa tcagagtact ggcaagatac ggtgttttta gtgattgcag
    11581 atcacgattc aagagccgtt ggcgagcatt tagtgccaat tcaacacttc catattccgg
    11641 cacttttatt aggcgaacac attcagccaa gaacggataa ccgtttagtg agtcagctcg
    11701 atatgccgac aacattactt tctgtggcgg gaataagtgg tcaatatccg atgatagggt
    11761 atgatttaac gcaagacatt gacccaaacc gtgcctttat gcaatatgat gcgacacagg
    11821 cgatgatgcg gggaaatgat gtggtggtaa tgcgaccaaa tttaccgata gaaggattta
    11881 gctacgataa agctaactat aaactcaccc ctaaagaaca gccggaatcg tttaaaaaag
    11941 aggcgttagc acacgcattg cttggcagtt atttatataa aaatcagtta tataaattac
    12001 cggaagaaac taagcagtaa ggcaaattaa aagagagggc tgcaatattt ttattaaaaa
    12061 tgaccgcttg ccctctctca aatttgttca ttactttaag aataatgctg ctgcaccacg
    12121 agtgccacct gagtcgccgt gaattgcttt tttgatgacc ggcactttcg ctgaacgcat
    12181 taagtgtttt ggtaatgctt ttggtagagc ttggtagata taatcaaagt tggataaacc
    12241 gccaccgaat acaatcatat ccggatctaa aatcgtaatc agattgctga tactgattgc
    12301 cattagctca atatattttt cgacaaatgc caccactttg gtttcttgtt ggtagaagcg
    12361 ttcgataatc gctttcgccg agattttttc tcctactaaa tcattaaaca gtaactcaaa
    12421 accccgccct gaaatatagg tgtctaaaca ggcttggttg ccacaaccac attcataaat
    12481 tggggcttta tcccagtcga gcaatttaag tgcgtggtag ttaagttgca tatgcccaac
    12541 ttcgcctgcc attccgattc tgcctgaatg aattttaccg tcaaacacaa aaccaccgcc
    12601 aaaaccggtg ccgataatca accctaatac ggtggaataa ggcttattac tttcgtccca
    12661 cgcttcagac agggcaaagc agttagcatc gttttcggca cgcacttcac gccctaaaca
    12721 ttcggctaaa tctttcagaa tcgttttgcc gtcagccact cgaatattgg taatttccgc
    12781 aatgccggtt tcacggttta caaagcccgg aatgcccagc ccgacagtgc ctttttcgtc
    12841 aaattttgta tcggcagtct caaccagcgt tttaattgcc gtcagccaat cttgataatt
    12901 tgtctgcggt gtcggcacac gttcgctgtg tagtttttct agtttttcat taaaagcggc
    12961 taattcaatt tttgtgccgc ccacatctaa gccataataa attgccataa ttacgctctc
    13021 ttattgcgat tgattgattt tatgcggttt ataaattgct aaatcattaa tccatttctt
    13081 cattgatttt tgctgggttt tcgtattcgc aaaattaaaa gttttccgtt tattattaaa
    13141 tttggtgcca agtagaatga ctttcgtttc actttcggaa acttcataca agaagtaaac
    13201 attatcaatg acgatatatt tgtaaccggt atcgccccaa aaaggtctga tgttgggtaa
    13261 attagtgaga attttatgca ggaaatcgag ttcttcttga gtaagataag acttaatgaa
    13321 gtgtttcaat aagcctttct ctaagtttgg gtcttttggt tctgcaataa aaacttgata
    13381 catagcaagc ctattttttg aagtcaaaag gttgaatagt gttatttaag attgcctccc
    13441 aagtatcagc actagcctgg gcggcaaatt gtctttcctc aatactaaac ggatagtcat
    13501 ctttttcttt atcgcttaat agttcagcta ttttttctat cgacatttcc attctaagct
    13561 ccttatttta ggtacttatt caataatttg tattgtatat tgatttggct aaattgccaa
    13621 ttaaggcttt ttagcaaacc attttttact ttccaaccaa ggcacaaacc acacaaataa
    13681 ggctaaattg attagaaacg caattaaact actggtgaat aaatcaatcg ggtagtgcat
    13741 acctaaacgc aagcggctaa tcagcattaa cgccgcccaa ctgattgcaa aaaaatgagc
    13801 caaaacgacc gcttgtcctc gcatattacg gcagaaacct accaccaata atagccaaga
    13861 aaccgcaaaa atagtgtggc ctgatgggaa agaatagccc acttctttgg cttggtgagc
    13921 ggtaacaaaa ttttgatttg caccgatttt ttccattacg gcaagcccac gttgctcgcg
    13981 tgttaattga taaaaatcgt ctgttttatc ttggaaaaga gcagtgacaa atggacgagg
    14041 ctcttgaaag gttgatttca tcgctgtttt gattgcttga gtggctacca ttgaagcgac
    14101 acacacggta atcacaaaaa ttaaagagta gcgtttgcga accaccctcg ccagtaataa
    14161 ggcgaaaacc acacaggtga tgatggcata aggtacggag ccggtttcgg tgattaaata
    14221 taaaaagtag tcgaaatcaa attcagcgta ttgaattgcc agagaccagt gccaatttgc
    14281 cagccaagtc accacaggaa tgagcaacat tgcaagggta taaagagaga attttttaaa
    14341 catagaaagt cctataaaaa accgcttgaa tacaagcggt tgaatttaaa catatttttg
    14401 caattagatc gctttagttg cttttggcaa gctgattttt ttctcttcgc tttggcggta
    14461 aagcactaaa atgtgcccaa tggtttgtac ttcagccgct ttagtttcac gcagaatggc
    14521 atcaataatt aattgtttgg tttcacgatc agccccggca actttaactt taatcagttc
    14581 gtggtggtct aaggcgttat cgatttcagc taacacgcct tctgttaagc cgttaccccc
    14641 cagcatcacc actgggctta aatgatgagc caagcctttt aggaattgtt tttgtttggt
    14701 tgttaatgta attgacattc tttttccgtt aatttgtttt gttgataaaa tttggcgtag
    14761 aaaatagcac aaaaaccact atttttaaag taaaatgcaa cgaatagcag aaaaacacac
    14821 gacaaaaaga gagaaggata aactatgccg acattaagtg ttgcgatgat tgtcaaaaat
    14881 gaagctcaag atttacgggc ttgccttgac acagtaaaag attgggtaga tgaaattgtg
    14941 attttagata gtggcagtac tgatgaaaca ccacaaattg ctgctgaata caacgcaagg
    15001 ttctacacta acaccgattg gcaaggcttt ggtaaacagc gtcagttagc tcaacaatat
    15061 gtgaccagtg attatgtact ttggcttgat gcagatgagc gagtgacagc cgaactgaag
    15121 caatctattt tacaagcggt caaaaatgat gaaaaaaata ccgtttacaa aatcagccgc
    15181 ttaagtgaag tatttggcaa acaaattcgc cactcgggtt ggtatccgga ttgtgtggtg
    15241 agattgtatc gcacggaatt tgccaaatat ggtgatgaat tggttcacga aaaagtacat
    15301 tacccaaaag aggcaaaaat ccagaaacta caaggcgatt tgctgcattt cacctataaa
    15361 gatattcacc actatttggt aaaatcggca agttacgcta aagcgtgggc aatccaacgg
    15421 gcaaatgcag gcaaaacagc cagcttattt gatggcgtga ctcacgccat cggctgtttt
    15481 gtgaaaatgt atctattaaa agccggtttt ttagatggca agcaaggatt tttattggca
    15541 gttctttcgg cacattctac tttcgtaaaa tatgcggatt tgtggaatcg tactcggaaa
    15601 tgatgctttt cagtactctc tccctctagt gggagagaga tagataaaat tgcgtttagc
    15661 aattttatca gagagaggga gtattccaca ttctccgaat aaacggtact atcgttttta
    15721 atgcctcaga aatagaaatt aaagttaaat tcatttgtga ggctttatca gacggtgggc
    15781 aaaatgcaat gtgtttattc gcatcattaa tcggtttcca acggcgtgga atggcagaaa
    15841 gtcggctcgg gtagaagcca atagttggaa tatttaacgc cccactaata tgtaacggac
    15901 cggttgagcc ggcaataaac aaatctgcac acgcaagcga atgtgtaaaa tcttgtaacc
    15961 cctcattttt atcataaacc accacgttgt tgtgattgat ttgttgagct aactgctgag
    16021 ctttttcgct ttcgcctttc cctgcggtga gtaccacata gcaatcaaat tctgccaaaa
    16081 taccttgaat taactctgca tactgttcta tagataaatt atttgccgaa ccaccggttg
    16141 aactatgcac aaatacccac tttttaacgg tggtaatatt gagttgttcg cataattttg
    16201 ctttttgtgc ctggacggtt tgctgttcaa cacttaagta tggcgtgtga ggttcaacaa
    16261 tcggtatatt gtgtttgctt aaaaaagcac gggctaaatc caaattatat tcggattccg
    16321 cttttgctga ttgagaacgg cgttgggtta aacggtggtt atataaaaat tggaataatt
    16381 tagttgctgg agcgaggcgg tatttaatcc cactttgcca ggtgagtttg gcattatgcc
    16441 agtctgaaac aaaacaaata accgcatcaa attgctcatt tttaaccgct tgtaaaatac
    16501 ggttaaattc atttttatct tttttattcg ccgtatcaat aataatctta tcaatagaag
    16561 ggcaaagctc ggcaagtgga gcagtataac ttggcacaag agcggtaatt tccacattcg
    16621 gcattgattg cttcaacata gcaaatgccg gaaagcagac cataaagtcc ccaattttat
    16681 cattacggat tacgagtagt tttttcattc tgattttctc tattgctttt aggttttcag
    16741 taaattataa gcggtcaaat ttcacaaaaa atttgcaaat catcgggcta aattttagat
    16801 gaatttttac tacttttcag ctagaatatt ttgcttgaac gtaagcgttt gcgtttgaat
    16861 atttttactt tcaaaattta taggaattcg tatgtcagca acaattttag aagctttacc
    16921 tttagggcag aaagtgggta tcgccttttc aggcggttta gatacctcag cagcactgtt
    16981 atggatgcgt aaaaaagggg cagtgcctta tgcgtacacc gcaaatttag gtcagccgga
    17041 tgaagatgat tacaacgcaa ttccgaaaaa agcgatggaa tatggggctg aaaatgcacg
    17101 attaatcgac tgtcgccaac agttagctca cgaaggcatt gccgcaattc aatgcggtgc
    17161 tttccatatt tcaaccggcg gtattccgta tttcaacacc acgccacttg gtcgtgcggt
    17221 aaccggcaca atgttggttg cggcaatgaa agaagatgat gtgaatatct ggggcgatgg
    17281 ttcaaccttt aaaggtaacg atattgagcg tttctatcgt tacggtttat taaccaatcc
    17341 aaaattaaaa atttataagc cgtggttaga ccaactattc attgatgaat taggcggtcg
    17401 cttggaaatg tctaaattct taatcgaaaa cggttttgac tataaaatgt cggtggaaaa
    17461 agcctactct acagactcga atatgttagg tgcaacccac gaagcgaaag atttagaaga
    17521 attaagcacc ggtatgaaga tcgtaaaacc gattatgggc gtggcattct gggacgaatc
    17581 agttgaaatt aaagcggaaa ctgtttctgt tacctttgaa gaaggtgtgc cggtggcgtt
    17641 aaacggtaaa cgtattgatg atgccgttga attaattatg gaggctaacc gcattggtgg
    17701 cagacatggt ttgggaatgt cagaccaaat cgaaaaccgt attatcgaag cgaaatctcg
    17761 tggtatttat gaagcaccgg gaatggcatt attacacatc gcttacgagc gtttagttac
    17821 cggtattcac aacgaagata cgattgagca atatcgcatt aacggattac gtttagggcg
    17881 tttattgtat caaggccgct ggtttgaccc gcaagcttta atgttgcgtg aaacggctca
    17941 acgttgggtg gcaaaagcga ttaccggtac ggtcacgctt gagttacgcc gtggtaacga
    18001 tttcactatt cttaacactg aatcgccaaa cttaacctac gaagcagaac gtttaagtat
    18061 ggaaaaagta gaagatgcac cgtttacgcc ggcagatcgt atcggtcaat taacaatgcg
    18121 taacttagat attgtcgata cccgtggtaa attagcagtt tataccgatg caggtttatt
    18181 ggcggctaat aatagcgtga ttccacaact tggaaaaaaa taatcagttg atctccattc
    18241 tgataacttt tagaatttag cctaaaagct gccccaaagt taatgctttg gggcttttat
    18301 tttcaaagcg tgatgatgaa attagttgaa atttttacag acggttcttg tttaggcaac
    18361 cccggcaaag gggggattgg tattttgctg cgttataacg ggtacgaaaa aaccgtgagc
    18421 aaaggctatt ttcaaaccac caataaccga atggaattgc gggcggtgat tgaggcttta
    18481 gcaatgctaa aagagccttg taaggtgcaa ctcaatagtg acagccaata tatgaaaaac
    18541 gggatccaaa aatggatttt taactggaag aaaaatgact ggaaaaccag cgataaaaag
    18601 ccagttaaaa ataaagattt atgggttgct ttagaccaag aaattcaacg ccaccaaatt
    18661 gaatggagtt gggtgaaagg gcattcagga catcgtgaaa atgagatttg cgatgagctg
    18721 gctaaacaag gagcaaataa tccaacgttg gacgatgttg gttatattgc agactaggtt
    18781 ttacaagcgg tcggattgtg taaatttttt accgaattta accgcttgta tttgcataaa
    18841 gcacttattt ttagcgaaat agtgctacgc aaacgttttc tttttttgta gaataggaga
    18901 gctttacgct ttaaggtgtg ttatggttga tttaattgtt atcggaatca ttgctttttc
    18961 agtgcttatc agtttatggc gaggttttac caatgaagca ttatcgctac ttggttgggt
    19021 aatcgccttt tttgtggcaa gctcgtttta tccttattta agcggttatc tcacacaaat
    19081 taattcggtt tatttgcaaa attcagaatt tttacgcaat ggtattgcag cggcgattct
    19141 gttcattttg actttaattg tttgtggtgt gatcaacggt ttattaggca aagtgattga
    19201 tttgagcgga atgtctatca ccgaccgtat tttaggcggt gcttttgggg cattgcgtgg
    19261 tattttaatt gtgtcggcta ttttattttt cttagatacc tttacccaag caagccaaac
    19321 ggaattatgg aaatcatcgc tattaattcc gcattttgac tttattgtga agtggttctt
    19381 tgaacagtta caagcaaatt ccagtttttt aaacacgact aaataaagag gtttttctat
    19441 atgtgcggca ttgtcggtat tattgggaat tcgccggtga atcaggcgat ttatgatggt
    19501 ttaacattac ttcaacaccg aggacaagat gccgcaggta tcgtcaccat agacgatgaa
    19561 aatcgtttcc gcttacgcaa agctaacggc ttagtcagcg atgttttcca gcaagagcat
    19621 atggtgagat tacaaggcaa tgttggaatt ggtcacgttc gctacccaac agcaggtagc
    19681 tcaagtgtgt ctgaagccca gccattttat gtcaattcac ctttcggtat taccttagtt
    19741 cacaacggta atttaactaa taatgcggaa cttaaagctc gcttatacaa cgaagcccgc
    19801 cgccatgtga acactaattc tgattctgaa tcccttctta atatttttgc ttacttttta
    19861 gatctctatt ccactcagca tttaagccca gacaatatct ttgaaacggt tcgtaaaacc
    19921 aatgatagca ttcgtggtgc ttatgcttgc attgcgatga ttatcggaca cggtatggtt
    19981 gctttccgtg acccattcgg tattcgcccg ttagtgctgg gtaaacgtga aatcgagggt
    20041 aaaaccgaat atatgtttgc ttcggaaagt gtggctcttg atgtagtggg gtttgaattt
    20101 gtgcgagatg tgctgccggg tgaagcgatt tatgttacct ttgatgggca attacattcg
    20161 caaatttgtg ccgataatcc aaaactgaat ccttgtattt ttgaatatgt ttattttgcc
    20221 cgtcctgatt ccgtcattga tggcgtttct gtatattctg cacgagtgca tatgggcgaa
    20281 ttattaggtg agaaaattaa acgtgaatgg ggacgaatta tcgatgatat tgatgtggtg
    20341 atcccgattc ctgaaacctc aaatgatatt gcggtacgta ttgctaatat gttgtataaa
    20401 ccctatcgtc aagggtttgt taaaaaccgc tatgtagctc gaacttttat tatgccgggg
    20461 caagcacagc gtaaaagctc ggttcgccgt aaattaaatg cgattgcctc tgaatttaaa
    20521 ggcaaaagcg tgttactggt tgatgattct attgtacgag gtacaacgtc tgaacaaatc
    20581 gtggaaatgg cacgagcagc tggtgcaaaa cgggtttatt ttgcctctgc cgcaccggaa
    20641 attcgctacc cgaatgtgta tggcattgat atgccgactt gtgaagaatt agtggcttat
    20701 gatcgctcag tggaagaggt tgcacagatg ataggggtgg ataaattgat tttccaagac
    20761 cttgaagcac tttataagtc tattcaactg gaaaatccga ctattcatcg ctttgatgac
    20821 tctgtattta caggagaata tattacaggt gatgtagata aatgctattt agacagtata
    20881 gcaagatctc gaaacgataa agcaaaagca gaggcggcaa aacaagccac caatttagaa
    20941 attcat
//
LOCUS       DANLRK010000019        20841 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.019, whole genome shotgun sequence.
ACCESSION   DANLRK010000019 DANLRK010000000
VERSION     DANLRK010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 20841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20841
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.019"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            <1..619
                     /locus_tag="PXA33_000557"
     CDS             <1..619
                     /locus_tag="PXA33_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2572 family protein"
                     /protein_id="HDL5119921.1"
                     /translation="VLFLSKEKWLGQQNITHFYREKYLSDQYNFLALYPQDKTQICQK
                     FQKEQISQADLSDKQFRFLQYQFACQFHSLFKDKKPTKEKYIHFTHLADYLDLSNVPN
                     QEIYTIHSLAELPSSSEREPKIVLAKNAINENLPQEFYGIVITDYLFDITGKKMYGTL
                     YSSYDNAREERNLTFKKEVLAELEARYSAWRYLPYSEHLMGAVDD"
     gene            612..905
                     /locus_tag="PXA33_000558"
     CDS             612..905
                     /locus_tag="PXA33_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5374 domain-containing protein"
                     /protein_id="HDL5119922.1"
                     /translation="MIKYKAETALSLLVAITLFAIVVLSFSQWQSAQNHRLNQHFQQQ
                     QAVMILENQLALKLAGLDCESSITQNQIRYQIQCSGNRLTIRFPLGKIELNND"
     gene            954..4244
                     /gene="recC"
                     /locus_tag="PXA33_000559"
     CDS             954..4244
                     /gene="recC"
                     /locus_tag="PXA33_000559"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HDL5119923.1"
                     /translation="MFTLYQSNQISSLAEMLVKIQQVNPLEDPFEPETILIQSQGMAQ
                     WLQMQIAELNGVMGNCDFLYPTTFLWQQYRLLFPELPKENIFERSSLVWRIMRLLPSC
                     LTLSEFAPLKRYLGDQPNKEQYQLKLYQLSAKIAELFDQYLVYRPHWLVSWEEGNLQA
                     IENEIKIATAAKPIDFDLIRADMKWQSHLWQQIVSDLQSESDEQVFTTSHRAYLQQNY
                     FAKLDDLTETEKAKLPKRIFIFGISSFSPLQLAVFKKLSEHCDIHLFFFNPSEKYWGD
                     SVEEKVWQKLALSHKISQDDLDNLLAEQRNKLLNLWGKQGRDFLAQLIEFEPNTMDVF
                     VEPEENSNLNKLKKQIFSLENDATITDLTADNSLQIHSCHSPMREVEVLHNQLLNLFE
                     QDSTLLPKDIIVMSPDINKYAPYIDAVFSRYNTGNRDPRYIPFSLSDQAFTQVDPIIH
                     SFLQLLAMKESPFNAEELFDLLEVNAIQTRFGFSTEDIHTLRVWVRNAGVRSGVEIEQ
                     PHWQNYNSWENGLNRLLLGSSLKEEQGIWENTLAFGDSYGLNAELVGRLASFIEALLA
                     WVNFLQTPQPLAAWQQAIKNLTADLYQENAENAASILLLNNVNDQIIEQISATHFSDD
                     LQIEVLALLFEQQLSQHSQQLNFLVGRVNFATLLPMRAIPFKVICLLGMNEADFPRQQ
                     QINSFDLMQYAPKKGDRAKRDDDRYLFLEALLSAQQMFYISYIGQSQKDGKEMLPSIL
                     VSQLLDAMNEALTPECKQQLSDEVEVLVRKQPLSVFSKKNFTHPRDHAYNAEWIIKPT
                     VAVQDFLSEPLTNESVASLELDELISFVQNPIRFFFLKVLGISFNTYDDTIDESEIFE
                     MSGLDNYRILNELLTINLAQQQRFFAEEQLKGNLPASYFAQIATQDLIGKITEMQAAL
                     SHYLASESRILSVNREVELERQTLTLNGNIANLYQNEVVLWRVGHLRDKDKIQTWIYH
                     LLLCAYFPEKTVKFYYREGEQVGVLSFETVSQIEALSQLSIYLNAYLDGLSQAQLVIY
                     DRIEEYLKGDGTNSEQVLIDLAEKDERDGSYLHRLLAQTKELDSQAIHQRTQSWFSLM
                     NQKIAKGD"
     gene            4380..7325
                     /gene="ptrA"
                     /locus_tag="PXA33_000560"
     CDS             4380..7325
                     /gene="ptrA"
                     /locus_tag="PXA33_000560"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HDL5119924.1"
                     /translation="MKLSFLTQFVAAAFALNVMTYPAFAEVAKNSEKTTASQPQSQLG
                     FELIKQSINKSPNDKAIYQGIKLDNGMEVLLISDSQANKSLMSAIIPIGSMDDPISQQ
                     GLAHYLEHMILMGSKNYPETNSLDGFLTKNGGMNNASTASHRTAYYLQVNNEAFSEAV
                     TRLADTLAFPLLLETNAKKEVNAVNAEMVRAKSHDGHLLHSVNLATANPAHPATKFAV
                     GNKETLSDKPDSKLQTELEQFYKQHYSANLFKVVLYSNQSIEQMAKLAANTLGKMENK
                     HFSIPQTAEPRYRDEDKSVFIQYKPVKSNKMLVIGFDFPNDEMQFKHKTGKYIAYMLN
                     NNTDGTLSDYLIKQGLSDGGIEAVSSANIGRDRGSFDIYIELTDKGLSEQDQIISLVF
                     QQIEKIKQEGIQESYFSEVKESLKQDFQHLQVEKNMDFVEFIADQMLHFPLEHVIDQP
                     YLTETMDNEAIKAKLAAMNIDNARIMLVSDTAKTDKKTPYFEAGYSVAKITQEQKQKW
                     LDFSKNPQLNLPELNPYFTTDFTLNKVETGLTKPKLINDQAGNLIYTMPSVYFTNEPK
                     AKIGVGFNIRPRKDDLKSLTAASILGYMNNLAQTKLDFQSSVAGMNLSLAPAANGLAL
                     SAEGYTQNLAKLMLDSVKQFSRFEFNEDVLAQAKQRLHQELDRNEKENSLNQANAALS
                     RFASYPYFDVPKQREMIDSITLADIEQLRQRILQQSSGFKLLSVGNLSAEQVSALAED
                     LQAVIKNQKSANATGQYVDVSTSQRKINVIKSVPNEDNALLMAFMPNGYNELESLTRA
                     KLLRSIISRWYFDDLRTDKQLGYVVYATDNIIGKTAGIQFMVQSPNTTPAGILEHNER
                     FFTQSFERLKNLSDEEFGKYRDSLVEKLQYKPESLAEEFSEFGSDFSRNNPKFDRLQQ
                     VIELTKALTKADIVAFYQKAVIEKQGFVFVSQAVGTKTKAEDVAKFDGFEQIESIEKL
                     QREFEIKDY"
     gene            complement(7374..9020)
                     /locus_tag="PXA33_000561"
     CDS             complement(7374..9020)
                     /locus_tag="PXA33_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005606119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL5119925.1"
                     /translation="MKFPTLTKISASVLAVLALAACDDKSNENKPTAAAAKPQTEKTF
                     VNCVSRSPTGFSPILMMDGLSYNASSQQIYNRLVEFVPGTTDIEPALAESWEISEDGL
                     TYTFKLRKGVKFHSNKDFTPSRELNADDVVFSFNRQLDKNHPYHTVSNGTYPYFNAMK
                     FPTLLKSVEKVDDSTVRITLTKRDASFLSSLGMDFTSIYSAEYADKMMKAGIPEAVDT
                     TPIGTGPFVFSGYVLDQASRYVANKEYWKGKPDIDRLIFEIVPDATTRYAKLQSGQCD
                     LMDFPNATDIEKMKNDPKINLLSQPGLNIAYVAFNTEKAPFDNVKVRQALNLAVDKKA
                     IIDVVYQGNGFAAKNPLPPTIWGYNDSLADSEYNVEKAKQLLAEAGFPNGFETELWVQ
                     PVVRASNPNPRRMSEIIQADWEKIGVKAKLVTYEWGDYIKRTKAGELTAGTYGWSGDN
                     GDPDNFLSPLFGTPNIGNSNYARFTNPELDALLEKALGSSDKAERTKLYEQAQVILRE
                     QAPWINVAHSINFAPTSKRVEGYKQSPFGYTYLYPVKIVD"
     gene            9096..11186
                     /locus_tag="PXA33_000562"
     CDS             9096..11186
                     /locus_tag="PXA33_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent
                     hemoglobin/transferrin/lactoferrin family receptor"
                     /protein_id="HDL5119926.1"
                     /translation="MIFANQSIELDTVNVIATRDPSKFADTPQKQTKDALLVKQATSV
                     AAALKALPNVDVQGGARAIVQKPSIRGLSDNRIVQVIDGVRQNFDLAHRGSYFVPMSL
                     IQEIEVIKGPSSSLWGSGALGGVVAMRTPNALDLLKNNDKFGAKIRQGYQTANNLSET
                     EASVFAANDRFDALLSGFYNNADNLRSGEGKELNNTGYKQAGGLVKFGWQINDTHRLE
                     LSHRHSQFKQTAPGNNEVENQFTDNDVSAQISAWHAANPGNPSNPVYLAKMLAFYGQL
                     HSSLGSVSYLSDQKITDKSTSLNYYFNPSNPYLSTQVTLYSNSTTENEHRKISGVEDQ
                     TKLSTRGVNIRNHSELSWFSLVYGVDYMLDKVATQRGRNNTDAQYRANPYDAKSKTTG
                     AYLIAHIPLFDEKVVFSPSVRYDRFDTSSETVKYKDSHWSPAAKLTWKATNWLDLTAK
                     YNEAFRAPSMQERFVSGSHFGTTVRGLPINNIFLTNPNLRPETAKNKEVTFNVHFDNV
                     ISQGDKFNFEATYFRNDVKDLINLQVFKANPTSPMEFIPTRSQYQNIANARLSGIELQ
                     TRYQTERLAVFANYGSTKGKDKDSGEALSNIAASKIGFGVNYAVVQDKFTVGANVTRY
                     QAQHRVPKNHGVTYQGYTLTDLHATYAPLKGEWKNLRLDVAVENLFDKKYQPAFSLME
                     GSGRNVKLSAAYSF"
     gene            11257..12945
                     /locus_tag="PXA33_000563"
     CDS             11257..12945
                     /locus_tag="PXA33_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005694040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HDL5119927.1"
                     /translation="MKFYPSLIASAVSFAVVLSGQAAVNVPDAGSLQRELDQRQAQQK
                     VKPSGEWFNQAAKAPYTATDGQELTFPLTQVQVFDRTNQEISTPELAPILANYVGKAV
                     SLTDLSNLANEITEFYRSHNYLVAKAILPPQEIEQGKVKILLFKGNVGEVRLQNQSAL
                     SDKFVARLANTTVNGSEFILKDELEKFALTMNDVPGVNTGLELSAGKQAGEANLLIKL
                     QNAERFSGYVSADNQGNKNTGRYRLATGIKANNLIGWGDELKVDLMSSNNANLKNARL
                     DYSSLIDGYATRLGLTANYLDYELGGNFKALQSQGHTHNLGAYVLHPTIRLPDFRLNT
                     KVSFNHQRLTDKQQAVNVAQKRQVNSLTVGINGSWNSLKDGTSYFSLSALFGNLANHT
                     NEQAHYVATDFQPKSRFTVYQYSLSHEQALPKSFAFNLGVNGQFADKTLDSSQKMLLG
                     GLYGVRGHQAGVASVDEGHLVQTELKHYLPLFSKSLLTSSVFYDYGWGKYYKHSGFLA
                     RGVNNSVKLQSIGAGLSLSEAGSYAINASVAKPLGKKIENADKHQFWFSAIKTF"
     gene            12957..15773
                     /locus_tag="PXA33_000564"
     CDS             12957..15773
                     /locus_tag="PXA33_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005694039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HDL5119928.1"
                     /translation="MFKRNAISLCILTACSGLAYANTADLPQGERVRVGTATIERTTD
                     KMTVNQSSNKVQIDWNSFDIGKNKEVEFKQPTEMAVAYNRVTGGNASQIQGKLTANGK
                     VYLANPNGVLITKDAEVNVGGLLVTTKDLEKISENGTKDKFSRKLAKEGKILNQGKIT
                     AKGERAFVVLVGDDVANEGEINAKNYYHKETKKVTECYDDSWSLVKQCYDFDKEIVKS
                     TSGEVHLSSGENVTFTLSDRYIDVALDDNTVNSIIKNDGAIIANGDITLTAKGRNQAL
                     DSLIINNGVLQANKVQNKNGTIILSAGETQLHEKSQIKGEKVSFAEASYYGPTEDIKV
                     VSKTGSKVTAPVIDFKGKTVNIQGEFGREDSTDLYNDELQILKTTVNIEVPNTEDIRI
                     ADTDNGGSGSFIQAGALASLLANNGRVNVQGKGFDVSGKLNVNAFKQTDSVLKFINQA
                     GINIHNADIHANGRLFFVTNLPNDTDFQSNIRITDSKINLGNGSMGFGRSSKRWEMHD
                     VVRRADTELRKKFNLDMKNVELNQVDDFVVAGGFQQVNLDNVKARGKTNFYLDSGNSR
                     MFVGEQIYEYGVTDIEKRTQLANLDQRRKRWNSDNYDETDLNWEYNQRFDGFAAKHNH
                     RAPIADTEINIANSDISLDNGFVHLMAEKINLDDSKINITFNQDNSLDESTQINRLGL
                     NGKVSMKNSHINVVGDEKDGISPARHYATMFLVGELLGEKSSIFLKSHQGAVLRTDSN
                     VTIAGKNSKDDLKITAITTGKKMGNEIIGPDGLPTSEDNDANASNTAFAIGYEPNATA
                     IVENASITALAPNGGLAYTGTSKTQIDAKDNATFTFFEKPRAGYDKLLGDIKGNPDKL
                     TPRDFARLYDNIKGIKASSLSEQQLGLADTAKKTSEHTLANSLDVENLVSVTVCDEQN
                     KCEERMLGNKNTNAKVIVGDIKFE"
     gene            15896..16027
                     /locus_tag="PXA33_000565"
                     /pseudo
     CDS             15896..16027
                     /locus_tag="PXA33_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587011.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(16082..16843)
                     /locus_tag="PXA33_000566"
     CDS             complement(16082..16843)
                     /locus_tag="PXA33_000566"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HDL5119929.1"
                     /translation="MKLNQAQIQNALICGQAVVFDEIDSTNEYLLTHHQRLENGTVCL
                     AEKQTAGRGRRGRTWYSPESENLYFSILWHYPMEQAADLPPLSLVVALIIAESLTVQN
                     VKDIQIKWPNDIYYQGKKMGGILLETQANRNGLDVVIGIGLNLGMAKVDENIVTQAWA
                     DLSQYHFNRNELVCRLAYELQKNLKIYPLVGFAHYAERWQSFDLFRHKAVKLITEAEE
                     IHGISQGINEQGELILKQGDNLRYFGIGEISLRAD"
     gene            complement(16855..17886)
                     /gene="murB"
                     /locus_tag="PXA33_000567"
     CDS             complement(16855..17886)
                     /gene="murB"
                     /locus_tag="PXA33_000567"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HDL5119930.1"
                     /translation="MMKHSLTPFHTFHLPAKATQIIEFTTVEQLLSEWQKAFNAQLPI
                     LILGQGSNVLFLEDFDGVVLVNKLKGITHHEDEHFHYLHVQGGENWHELVKWTLAHNI
                     AGIENLALIPGVASSAPIQNIGAYGVEFEQVCDFVEVLNLRSGELFKLSKSECEFGYR
                     ESVFKHQYRDEFAIISVGLKLAKAWKPVLSYGSLAQLNPETVTPIQIFDEVCAVRSSK
                     LPNPDEFGNAGSFFKNPVISNEQFAPIQAKFPAIPHYPQADGTVKLAAGWLIDQTGLK
                     DLQIGGAAVHTQQALVLINKENATGQDVLALAKTVRQRVKEKFGVEIQPEVRFIGKNG
                     EVDSEKITR"
     gene            complement(17990..18664)
                     /gene="crp"
                     /locus_tag="PXA33_000568"
     CDS             complement(17990..18664)
                     /gene="crp"
                     /locus_tag="PXA33_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010787261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HDL5119931.1"
                     /translation="MQDMTLSTAPLENVTPPPSIHDPAVEWFLTHCHIHRYPAKYTLI
                     HAGEDAESLFYIVNGSVSVYIKDDETKEMLLTNLGTGEFVGELSLFEEKVQQRTAYVR
                     TKGPCEIAEISYKKFKQLVHLNPDILMYLSAQMARRLRQTSRQVGNLAFLDVAGRIAQ
                     TLLNLAKMPDAMTHPEGMQIKITRQEIGQMVGCSRETVGRIIKMLEDEGLISAHGKTI
                     VVYGAR"
     gene            complement(18726..18944)
                     /locus_tag="PXA33_000569"
     CDS             complement(18726..18944)
                     /locus_tag="PXA33_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HDL5119932.1"
                     /translation="MIIPWQELEESTLHNILDSFILREGTDYGERELSLAEKRERLLT
                     QLKADKVVIVWSELHESLDIKDKKSFLG"
     gene            complement(18947..19552)
                     /gene="slmA"
                     /locus_tag="PXA33_000570"
     CDS             complement(18947..19552)
                     /gene="slmA"
                     /locus_tag="PXA33_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005624468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HDL5119933.1"
                     /translation="MTEPKIKMPKKSVVERRQQVLEVLISLLNSEEGMQRITTERLAA
                     AVGVSEGALYRYFPSKTKMFEALIEKIEQTLTSYIHANKRQDSSANSVRAILYVILEF
                     ARKNPGVTRILTGHALMFEEELLKARVAKFFDNLELQFANILQLSKLRERKSFEDERA
                     LAGYLVNFCEGQFLRLVRSNFSYNQHQHFEKQWAFIKPLFD"
     gene            complement(19564..20019)
                     /gene="dut"
                     /locus_tag="PXA33_000571"
     CDS             complement(19564..20019)
                     /gene="dut"
                     /locus_tag="PXA33_000571"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014550997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HDL5119934.1"
                     /translation="MKQIDLKILDSRIGNEFPLPAYATEGSAGLDLRAMLNEPLTVKA
                     GETVLIPTGISIYIADPNLAAVILPRSGLGHKNGIVLGNLVGLIDSDYQGELKVSLWN
                     RSQADFTVNVGDRIAQLVFVPVVQASFNIVQEFEQTDRGEGGFGHSGKQ"
     gene            complement(20053..>20841)
                     /locus_tag="PXA33_000572"
     CDS             complement(20053..>20841)
                     /locus_tag="PXA33_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HDL5119935.1"
                     /translation="LEIALKSLLAHHNHLSIFILSTGDISHHWIETLRPYLENRYSTL
                     RLGYLDSNNLANFNSNGYITNSTYLRYYIEELFEHSDSPYWIYLDCDLVVNGNITDPF
                     TNLDFSFGSIAAIADPYVNKLKNHPYIYKNYFNAGVFYLNAKHFSKQPFIQLTERFKE
                     NLPFGDQDILNYCFQYNWIELDRKYNYQTEHLLDDIAQQKISPNYIPHIIHFTGGIKP
                     LEGKFNSSYVENVASLFRFYHNTSWEILTELPLGTIQLKPFITE"
BASE COUNT         6285 a         4280 c         4496 g         5780 t
ORIGIN      
        1 ggttttattt ttaagtaaag aaaaatggct tggacaacaa aacattaccc atttttatag
       61 agagaaatac ctttccgatc aatataattt tttagcgtta tatccacaag ataaaacgca
      121 aatatgccaa aaatttcaaa aagagcagat cagccaagcg gatttatccg ataaacaatt
      181 caggttttta cagtatcaat ttgcctgcca attccatagt ttatttaaag ataaaaaacc
      241 gactaaagag aaatatattc actttaccca tttggcggat tatttagatt tatcaaatgt
      301 gccaaaccaa gagatttata ctattcattc tttagctgaa ttacctagta gcagtgaacg
      361 tgagcctaaa attgtgcttg ctaaaaacgc aattaatgaa aacttaccgc aagagtttta
      421 cggcattgtg ataaccgact atctgtttga tattaccggc aaaaaaatgt acggcacgct
      481 ttattcctct tatgataatg cccgagagga gcgaaattta acgtttaaaa aagaggtact
      541 ggcagagctt gaggcgaggt attccgcttg gcgatatttg ccatattccg aacatttgat
      601 gggagcggtt gatgattaaa tataaagcag aaacggcttt gtcgcttttg gttgccatca
      661 cgctatttgc gattgtggtg ttgagtttca gccagtggca atcggcacaa aaccacaggc
      721 taaatcaaca tttccaacag caacaagcgg ttatgatttt agaaaatcag cttgccctca
      781 aattagccgg gttagattgt gaatcaagta ttactcaaaa tcagattcgc tatcagattc
      841 aatgctctgg caatcgctta accattcgct ttccgttagg caaaattgag ctgaataatg
      901 attgaatcgt ccctcgcttt ggaggacaat aattcaatta gcgaggcgtt attatgttta
      961 cactttacca atccaaccaa atttcatcac tggcggagat gctggtgaaa atccagcagg
     1021 taaacccact tgaagatccg tttgagccgg aaaccatttt gatccaaagt cagggaatgg
     1081 cacagtggct acaaatgcag attgctgaat taaacggtgt gatggggaat tgcgattttc
     1141 tctaccctac tacattttta tggcaacagt atcggctgtt gttcccggag ctgccgaaag
     1201 agaatatttt tgagcgtagt tcgttggttt ggcggattat gcggttattg ccgagttgct
     1261 tgacactatc ggagtttgct ccgctaaagc gttatttagg tgatcaacct aataaagaac
     1321 agtatcagct taagctctat caactttctg ccaaaattgc cgaattgttc gaccaatatt
     1381 tggtgtatcg cccccattgg ctggttagct gggaagaggg caacttacaa gcgatcgaaa
     1441 atgagataaa aattgcaact gccgctaagc cgattgattt tgacttaatt cgagccgata
     1501 tgaaatggca aagccattta tggcaacaga ttgtatctga tctgcaatcg gaatctgatg
     1561 agcaggtttt tactacctcc caccgtgcct atttgcaaca aaattacttc gctaagttag
     1621 atgaccttac tgaaacggaa aaagccaaat tgccaaaacg cattttcatt tttgggattt
     1681 cgtccttctc gccattacag ttagcggtgt ttaagaaact cagcgagcat tgcgatattc
     1741 acctgttctt ttttaaccca agcgaaaaat attggggcga cagtgtggaa gaaaaagtgt
     1801 ggcaaaaatt ggcgttaagc cacaagattt cgcaagacga tctggataac ttattggcgg
     1861 agcaaagaaa taaactactg aatctttggg gcaaacaagg gcgggatttc ttagctcaat
     1921 taatcgaatt tgagccgaat accatggatg tctttgtcga accagaggaa aacagcaacc
     1981 tcaataaact caaaaaacag attttcagct tagaaaatga cgccactatt accgatttaa
     2041 ccgccgataa ttcactccaa attcattcgt gccacagccc aatgcgggaa gtggaagtgt
     2101 tgcataacca gctcctgaat ttgtttgagc aagatagcac tttgctgccg aaagatatta
     2161 ttgtgatgtc gccggatatt aataaatacg ctccttacat tgatgcggtg ttttctcgct
     2221 acaatacggg taatcgggat cctcgctata ttcctttttc attatccgac caagcattta
     2281 cgcaggttga tccgattatc cacagtttcc tgcaactgct ggcaatgaaa gaaagcccgt
     2341 ttaatgccga agaactgttt gatttactgg aagtgaatgc gatccaaacc cgttttggct
     2401 ttagtacgga agatattcat accctgcggg tttgggtgag aaatgccggc gtacgttcgg
     2461 gtgtagaaat cgagcaacct cactggcaaa actacaattc gtgggaaaac gggctaaacc
     2521 gtttattgct tggctcaagc ctgaaagaag agcaggggat ttgggaaaac acccttgcct
     2581 ttggggatag ctatggctta aatgccgagc tggtggggcg acttgcctct tttattgagg
     2641 ctttattagc gtgggtaaac tttctgcaaa cgccacagcc gttggcagcg tggcagcagg
     2701 caataaaaaa tttgactgct gatctgtatc aagaaaatgc cgaaaatgct gcatcaattt
     2761 tattgttgaa taatgtgaat gatcagatta tcgagcaaat ttccgcaacc catttttcag
     2821 acgatctgca aattgaggtt ttagccttat tgtttgagca gcagctcagc caacattcac
     2881 agcagcttaa tttcttggtc ggacgggtga attttgccac cttgcttccg atgcgggcga
     2941 ttccgtttaa ggtaatttgc ttgctgggta tgaatgaggc agatttccct cgccaacaac
     3001 aaatcaatag ctttgattta atgcagtacg ccccgaaaaa gggagaccgt gccaaacgtg
     3061 atgatgaccg ctacttgttc ttagaagcct tactttccgc tcagcaaatg ttttacatca
     3121 gctatatcgg gcagtcgcaa aaagacggca aagagatgct accgtcgatt ttggtttccc
     3181 aactgttaga tgcaatgaat gaagctttaa cgcctgaatg caagcaacaa ttaagcgatg
     3241 aagtggaagt tttggtcaga aaacaacctt taagtgtgtt tagcaaaaag aattttaccc
     3301 acccacgaga tcacgcttat aacgcagagt ggattatcaa gccgactgtt gccgtacaag
     3361 attttcttag tgagccttta accaatgaaa gcgtggcttc gctagaactt gatgaattaa
     3421 tctcttttgt acaaaacccg attaggtttt tctttctaaa agtgttgggg atttcgttta
     3481 acacttatga tgacaccatt gatgaaagcg aaatttttga gatgtcgggg ctggataact
     3541 accggatttt aaacgaatta ttaacgataa atttagcaca acagcaacgt ttttttgccg
     3601 aagagcaact caaaggcaat ttacctgctt cttattttgc tcaaattgcc acacaagatt
     3661 tgatcggcaa aattaccgaa atgcaagcgg cgttaagcca ctatttagcc agcgaaagcc
     3721 gtattttgag tgttaatcgg gaggttgagc tggaacgcca aaccctcacc ctaaacggca
     3781 atattgccaa cctatatcag aatgaagtgg tgctatggcg agtagggcat ttacgagata
     3841 aagacaagat tcagacttgg atctaccatt tattattgtg tgcctatttc cctgaaaaaa
     3901 cggtgaaatt ctattaccga gagggtgagc aagttggtgt gttgagcttt gaaaccgtct
     3961 cgcagataga ggcattatcg caattaagca tttatttgaa tgcttatttg gacggattat
     4021 cgcaagccca gttagtgatc tacgaccgaa ttgaggaata tctcaaaggt gatggcacaa
     4081 acagcgaaca agttcttatc gatttagcag agaaagatga gcgagacggc agctatctcc
     4141 atcggcttct cgctcaaact aaagagctgg attctcaagc gattcaccaa cgtactcaaa
     4201 gctggtttag cttaatgaat cagaaaatag cgaaaggcga ttaactacaa gcggtcgttt
     4261 ttggctgaaa atttgcaaaa tgagaaggcg aataataatt cgcctaccta taaaaataga
     4321 attttttccg tattttactg tcaatttatt actacaatgc taatttcaaa aggagatgta
     4381 tgaaattatc ttttcttacc caattcgttg cagcggcttt tgccctcaat gtgatgactt
     4441 accctgcatt tgcggaagtt gcaaaaaatt ctgaaaaaac gaccgctagt caaccccaat
     4501 cccaactcgg ttttgagctg attaaacaga gcattaacaa aagcccgaat gataaagcga
     4561 tttatcaagg tattaaactt gataatggaa tggaagtgct actgatttcc gacagtcaag
     4621 cgaataaatc gttgatgtct gccattattc cgattggctc aatggatgac ccgatttctc
     4681 agcaaggttt agcccactat cttgagcata tgatcttgat gggctcgaaa aactatccgg
     4741 aaaccaacag cttagacggt tttttaacca aaaatggcgg tatgaataat gcctcgactg
     4801 cttctcaccg cacggcgtat tatctacaag tcaataacga agcctttagc gaagcggtaa
     4861 cacgactggc ggatacgtta gctttcccgt tattgctgga aaccaacgcc aagaaagagg
     4921 ttaatgcggt caatgcggag atggtgcgag ctaaatcgca cgatgggcat ttattgcaca
     4981 gtgttaattt agccaccgca aacccggctc atcctgctac caaatttgcg gtaggcaata
     5041 aagagacgtt atctgacaag ccggatagca aattacaaac cgagttggag caattttata
     5101 aacagcatta ttctgcgaat ctgtttaaag tggtgcttta ttccaatcaa tcgattgagc
     5161 agatggcaaa attggcagcc aacactttag gcaaaatgga aaacaaacat ttcagcatac
     5221 cgcaaaccgc tgagccacgt tatcgtgatg aggacaaatc ggtatttatt caatataagc
     5281 cggtgaaatc caacaaaatg ttagtcattg gctttgattt tccgaatgac gaaatgcagt
     5341 ttaagcacaa aaccggtaaa tatattgctt atatgttaaa caataatact gacggcacgc
     5401 tttcggatta cttgattaaa caggggttgt ctgacggcgg tattgaggcg gttagcagtg
     5461 ccaatatcgg gcgggatcga ggctcgtttg acatctatat tgaactgaca gacaaaggtt
     5521 tgagcgagca agatcaaatt atttcattgg ttttccaaca aattgagaag atcaaacaag
     5581 aaggcattca agagagctat tttagcgaag tgaaagagag cctgaagcag gatttccaac
     5641 atttgcaagt ggaaaaaaat atggatttcg tggaatttat tgccgatcaa atgctccatt
     5701 tcccgttaga acacgttatc gatcagcctt atctgaccga aacaatggat aacgaggcaa
     5761 ttaaagcaaa attggcggca atgaatattg ataatgcccg cattatgtta gtcagcgata
     5821 ctgccaaaac cgacaagaaa accccttatt ttgaagcagg ttattcggtg gcaaaaatca
     5881 cgcaagaaca aaagcaaaaa tggctggatt tcagtaaaaa tccacagctc aatttgcctg
     5941 aattgaaccc gtatttcact accgatttta ccttaaacaa ggtcgaaacc ggcttaacta
     6001 aaccgaaatt gattaacgac caagcgggga atttaatcta tacaatgccg agcgtgtatt
     6061 ttaccaatga accgaaggct aaaatcggcg ttggctttaa tattcgcccg agaaaagatg
     6121 acttaaagag cctgaccgca gcgtctattt tgggttatat gaacaattta gcccaaacca
     6181 agttagattt tcagtcttcg gtggcgggta tgaatttaag tttagctccg gcggcaaacg
     6241 gtttagcctt aagtgcagag ggttataccc aaaatctggc taaattaatg ctagacagcg
     6301 taaaacagtt cagccgcttt gagtttaacg aagacgtgtt ggcacaggct aaacaacgtt
     6361 tgcaccaaga gctggatcgt aacgaaaaag aaaacagcct taatcaagct aatgcagcat
     6421 taagccgttt tgcctcttac ccttattttg atgtgccgaa acagcgtgag atgattgata
     6481 gcattaccct tgcggatatt gagcaactac gccagcgaat tttgcagcag agcagcggct
     6541 ttaagttgtt atcggtgggg aatttgtcgg ctgagcaggt ttctgctctg gcagaggatt
     6601 tacaagcggt cataaaaaac caaaaatctg caaatgctac cggacagtat gtggatgtga
     6661 gtaccagtca gcgtaagatt aatgtgatta aaagcgtgcc gaatgaagat aatgcgttgc
     6721 taatggcatt tatgccgaac ggctataacg agctggaaag ccttactcga gccaaattgc
     6781 tcagaagcat tatttcccgt tggtattttg atgatttgcg tactgataag caactaggct
     6841 atgtggttta tgcgacagat aatatcatcg ggaaaaccgc cggtatccag tttatggtac
     6901 aaagcccgaa taccacaccg gcagggattt tagagcataa cgaacgtttc tttacacaaa
     6961 gtttcgaacg tttaaaaaat ctatctgatg aggaatttgg aaaatatcgt gatagcctag
     7021 tggaaaaact gcaatataaa ccggagtctt tagcggaaga gttttctgag ttcggttcag
     7081 actttagcag aaataacccg aaatttgacc gcttgcaaca ggtgatcgaa ttaaccaaag
     7141 cactcaccaa agcagatatt gtggcatttt accaaaaagc tgtgattgaa aagcaaggct
     7201 tcgtgtttgt cagccaagct gtcggcacca aaaccaaagc tgaagacgtt gcgaaatttg
     7261 acggttttga acagatcgaa agcattgaaa agctacaacg agagtttgag attaaggatt
     7321 attagagagt tattagagag agttaggggc gaaaaatttt cgcccctcat tttttaatcc
     7381 acaattttca ccggatataa gtaagtatat ccgaacggac tttgtttgta gccttcaaca
     7441 cgtttgctgg ttggggcaaa gttaattgag tgagccacgt taatccacgg agcttgctct
     7501 cttaagataa cctgtgcctg ctcgtataac ttagtacgtt ctgctttatc agatgagcct
     7561 aatgcttttt ctaataacgc atctaactca gggttagtaa agcgagcata gttactgttg
     7621 ccgatatttg gcgtgccgaa tagtggcgat aagaagttat ccggatcgcc gttatcgccc
     7681 gaccaaccgt aagttccggc ggttaattca ccggctttgg tacgcttgat gtaatcaccc
     7741 cattcatagg tgactaattt tgcttttacg ccgatttttt cccagtcggc ttgaataatt
     7801 tcagacatac ggcgtgggtt cgggttagaa gcacgcacca caggctgcac ccataattcg
     7861 gtttcaaaac cgttcgggaa gcctgcttca gctaatagtt gcttcgcttt ttccacgtta
     7921 tattctgaat ccgctaagct gtcgttatag ccccaaatgg ttggcggaag tgggtttttc
     7981 gccgcaaaac cgttaccttg gtaaactaca tcaataatcg cttttttatc cactgctaag
     8041 tttaacgctt ggcgaacttt cacgttatca aacggagctt tttcggtatt gaatgccaca
     8101 taagcaatgt tcaaacccgg ttgagaaagt aaattgattt tcggatcatt tttcattttc
     8161 tcaatgtcgg ttgcatttgg gaagtccatt aaatcacatt gaccggattg taatttcgca
     8221 taacgggttg ttgcatccgg cacgatctca aaaatcaaac ggtcaatgtc aggcttacct
     8281 ttccaatact ctttgtttgc tacataacga ctggcttggt ctagcacata accgctaaac
     8341 acaaacggac ctgtgccgat tggcgtggta tctaccgctt caggtatgcc ggctttcatc
     8401 attttatccg catattccgc cgaataaatg gaggtgaaat ccataccaag gctggataag
     8461 aatgacgcat cacgtttggt taaggtaata cgaacggtgc tgtcgtccac tttttccacc
     8521 gatttcaata atgtcgggaa tttcattgcg ttgaaatacg gataagtgcc gttagagacg
     8581 gtgtgatacg gatgattttt atctaactga cggttaaatg aaaatactac atcgtccgca
     8641 ttcaattcac ggcttggagt gaagtctttg tttgaatgga atttcacgcc tttgcgtaat
     8701 ttaaaggtgt aagttaagcc gtcttcgctg atttcccagc tttctgccaa tgccggttca
     8761 atatcggttg tacccggcac aaattccacc aagcggttgt aaatttgttg cgagctggcg
     8821 ttataagata aaccgtccat cattaaaatc gggctaaaac cggttggcga acggctaacg
     8881 cagtttacaa aggttttctc ggtttgcggt tttgctgctg ccgcagtagg tttattttcg
     8941 ttgcttttat catcgcaagc cgctagagct aataccgcta aaacgcttgc actaattttg
     9001 gttaacgttg gaaatttcat attattctcc gcttaatctt aggattaact tacgaagatt
     9061 accttttcca caatgaggag taaagaacaa aaagcattat ttttgcaaat caatccatag
     9121 aattagatac cgtcaacgtc attgccaccc gtgatccaag taaatttgcc gatacgccac
     9181 aaaaacaaac taaagatgca cttttggtaa aacaagcgac gagtgtggcg gcggcattaa
     9241 aagcgttgcc gaatgtggat gtgcaaggag gggcccgtgc gattgtgcag aagccgtcta
     9301 ttcgtgggtt gagcgataat cgtattgtgc aggtgattga cggagtgcgt cagaattttg
     9361 atttggctca ccgtggttct tattttgtgc cgatgtcatt gattcaagaa attgaagtaa
     9421 tcaaaggtcc aagcagctcg ttgtggggga gcggtgcctt aggtggggtg gtggcaatgc
     9481 gcacaccgaa tgcgttggat ttattgaaaa ataatgacaa attcggggca aaaatccgtc
     9541 aaggttatca aacggcgaat aatctttccg aaaccgaagc ctcggtgttt gcagcaaatg
     9601 atcggtttga tgccctgctg agtggtttct acaataatgc tgacaatttg cgtagcggtg
     9661 aaggcaagga actgaataac acgggctata agcaagccgg cggtttagta aaattcggct
     9721 ggcaaattaa cgatactcat cgtttggaat tatcccatcg tcatagccaa tttaaacaaa
     9781 ctgcaccggg taataacgag gtagaaaatc aatttaccga taatgatgtt agtgcacaaa
     9841 ttagtgcttg gcatgcggct aatcctggaa atccaagtaa tccagtatat cttgcgaaaa
     9901 tgttagcctt ttacggtcaa ttacattcaa gtcttggtag tgtgagttat ttaagtgatc
     9961 aaaaaattac cgataaaagc acctcactta actactattt caacccaagc aatccttatt
    10021 tgagcacaca agtgacatta tatagcaata gtaccactga aaacgaacat cgaaaaataa
    10081 gtggtgtgga agatcaaact aaactaagca ctcgtggtgt gaatatccgt aatcattccg
    10141 aattgtcttg gttctccttg gtgtatggcg tggattatat gttggataaa gtagccactc
    10201 agcgtgggag aaataatacg gacgcacaat accgtgctaa tccttatgat gctaaatcta
    10261 aaactacagg tgcttattta attgcacata ttccgctgtt tgatgaaaaa gtggtctttt
    10321 ctccgagcgt gcgttacgac cgtttcgata cttcaagtga aacggtgaaa tataaagaca
    10381 gccattggtc tcctgctgca aaacttactt ggaaagcgac aaattggttg gatttaaccg
    10441 cgaaatataa cgaagctttc agagcccctt ctatgcaaga acgatttgtg agcgggtctc
    10501 attttggtac cacagtgaga ggattaccta ttaataatat tttcttaact aaccctaatt
    10561 tacgcccgga aacggcaaaa aataaggaag taaccttcaa tgtacatttt gataatgtca
    10621 tctcgcaagg agataaattt aactttgaag cgacttattt ccgtaatgat gtgaaggatt
    10681 tgattaatct tcaagtattc aaagcaaatc ctacaagccc aatggaattt attcccactc
    10741 gttcccaata tcaaaatatt gcgaatgcac gtttgagtgg tattgaatta caaactcgtt
    10801 atcaaactga acgtttggca gtttttgcta actacggtag tacaaaaggt aaagataaag
    10861 acagcgggga agccttgtcg aacattgctg caagcaaaat cggcttcggg gtcaattatg
    10921 ccgtggtgca agacaaattc acggtggggg caaacgtaac ccgttatcaa gcacaacatc
    10981 gtgtgccgaa aaaccacggc gtgacttacc aaggctatac gctgaccgat ctacacgcca
    11041 cttacgcccc attaaaaggc gaatggaaaa acctgcgttt agacgtggcg gttgaaaacc
    11101 tattcgataa aaaataccaa ccggcattca gcttaatgga aggcagcggt cgtaatgtga
    11161 aactcagtgc ggcttacagc ttctaatgtc tttaacggaa gacggcacaa gcggtcgttt
    11221 tccggttatt ttttgcaata ttaaaaggga gttactatga aattttatcc gtcgctgatc
    11281 gcaagtgcgg tcagttttgc cgttgttttg agtggacaag cggcagtgaa tgtgccggat
    11341 gccggctcgt tacagcgtga attagaccaa cgtcaagctc aacaaaaagt caaaccgagt
    11401 ggcgaatggt ttaatcaagc ggcaaaagca ccttataccg caacggacgg tcaagaactg
    11461 acatttccat taactcaggt acaagttttt gatcgaacca accaagaaat ctctaccccg
    11521 gaattagccc cgattttggc aaactatgtg ggtaaagcgg tttcgctgac cgatttaagc
    11581 aacttggcaa atgagatcac cgagttttat cgtagccaca attatctggt ggcaaaagcc
    11641 attttaccgc cacaggaaat tgaacaaggt aaggtgaaaa tcctgttgtt taaaggcaat
    11701 gtcggtgaag tgcggttaca aaatcagagt gctttaagcg ataaatttgt tgcacgcctt
    11761 gccaacacca cagtgaatgg ttcggaattt atcctaaaag acgaattgga aaaatttgcc
    11821 ctcacgatga atgatgttcc cggggtgaat accggcttgg agctttctgc cggtaagcaa
    11881 gcaggcgagg cgaatttgct gattaagctc caaaatgccg agcgtttctc aggctatgta
    11941 tcggcagata accaaggcaa taaaaatacc ggacgttacc gtttagccac agggataaaa
    12001 gccaataatc tgataggttg gggcgatgag ttgaaagtgg atttgatgag ttccaacaac
    12061 gctaatttaa aaaatgcgcg tttggattat tcgagcttga ttgacggcta cgctacccgt
    12121 ttgggcttaa cggcgaatta tctcgactac gaattaggcg gtaactttaa agccctgcaa
    12181 tcccaagggc atactcacaa tcttggggct tatgtgttgc acccgacaat ccgcttgccg
    12241 gattttcggt taaacaccaa agtcagcttt aatcatcaac gtttaaccga caaacaacaa
    12301 gcggtcaatg tggcacaaaa acgccaagta aacagcttaa cggtgggcat taacgggtcg
    12361 tggaattcgc ttaaagacgg cacaagctat ttctctctgt ctgccttgtt cggcaattta
    12421 gctaaccaca ccaacgagca agcgcattat gtggcaacag attttcagcc caaatcccgt
    12481 tttacggtct atcaatacag cctgtctcac gaacaagcgc tgccaaaatc ctttgccttc
    12541 aaccttggcg tgaacgggca gtttgcggat aaaaccctcg acagctcaca aaaaatgtta
    12601 ctgggcggtt tatacggcgt gcgtggtcat caagccggtg tcgcttcggt ggatgagggg
    12661 catttagtgc aaaccgaact caagcattat ttaccgctct tttccaaaag cctgctgacc
    12721 agctcggtct tctatgatta tggttgggga aaatactata aacacagcgg atttttggcg
    12781 cgtggtgtaa acaatagcgt gaaattacag agtatcggag cagggctttc gctctctgaa
    12841 gcaggcagtt atgccattaa cgccagtgtt gccaaaccgc tgggtaaaaa aatcgagaat
    12901 gcagataaac accaattctg gttctccgcc atcaaaacct tctaaggaga caaactatgt
    12961 ttaaacgtaa tgcaatttct ctctgcattt tgaccgcttg cagcggttta gcctatgcca
    13021 atacagcaga tttgccgcaa ggtgaaaggg tgcgtgtcgg gacggcaacg attgagcgca
    13081 caaccgataa aatgaccgtg aatcaatcca gcaataaagt acaaattgat tggaacagct
    13141 ttgatattgg taagaataaa gaagtggaat tcaaacagcc gacagagatg gcggtggcat
    13201 ataaccgtgt taccggcgga aacgccagtc aaattcaagg caagctgacc gcaaacggca
    13261 aagtgtattt agccaacccg aacggtgtac tcatcaccaa agatgcggaa gtgaatgtgg
    13321 gcggtttgtt agtgaccaca aaagatttag aaaaaatcag cgaaaacggc acaaaagata
    13381 aattcagtcg caaattagcg aaagaaggca aaattcttaa ccaaggtaaa attacggcca
    13441 aaggcgagcg ggcttttgtg gtattggtgg gcgacgatgt ggctaatgaa ggggaaatta
    13501 acgccaaaaa ttattaccac aaagaaacga aaaaagtcac cgaatgttat gacgattctt
    13561 ggagcttggt aaaacaatgc tacgattttg ataaagaaat cgtgaaaagt accagtggag
    13621 aagtgcattt atcctccgga gaaaatgtca cctttacttt aagcgaccgc tatattgatg
    13681 tggcgttaga cgataatacg gtcaatagca ttatcaaaaa tgacggggca atcatcgcca
    13741 atggtgacat taccctcact gccaaagggc gaaatcaagc cttagacagc ttaattatca
    13801 ataacggcgt attgcaagcc aataaggtgc aaaataaaaa cggtacgatc attttatctg
    13861 ccggtgaaac ccaattacac gaaaaaagcc aaattaaagg cgagaaagtg agctttgccg
    13921 aagcttctta ttacggtccg acagaagaca ttaaagtggt ttccaaaacc ggctcaaaag
    13981 tgaccgcacc tgtgatcgat ttcaaaggta aaaccgtgaa tattcaaggg gaatttgggc
    14041 gtgaagacag caccgatctc tataacgatg aactacaaat cctcaaaacc acggtgaata
    14101 ttgaagtccc gaatacggaa gatattcgca ttgcagatac cgacaacgga ggtagcggtt
    14161 cctttataca agcgggtgct ttagcctcat tacttgcaaa taacggacgg gttaatgtac
    14221 agggcaaagg ttttgatgtc tcaggcaagt tgaatgtgaa tgcctttaaa caaacggatt
    14281 ccgtgctgaa atttatcaat caggcgggca tcaatatcca caatgccgat attcacgcaa
    14341 acgggcgttt attctttgtg acaaacttgc cgaatgacac cgatttccaa tcgaatatcc
    14401 gcattaccga cagcaaaatc aacttaggca acggctcaat gggctttggt cgcagtagca
    14461 aacgttggga aatgcacgat gtggttcgac gtgccgatac ggaactgcgt aaaaaattca
    14521 atctggatat gaaaaacgtg gaattaaacc aagtggatga ttttgtggta gcgggcggtt
    14581 tccaacaagt taatttggat aacgtgaaag cccgtggcaa aaccaacttc tatctggatt
    14641 ccggcaactc ccgcatgttt gtcggtgagc agatttatga atatggcgta accgatattg
    14701 aaaaacgcac ccaattggcg aatttagacc agcgtagaaa acgttggaat tcggataact
    14761 atgatgagac cgatctcaac tgggaataca accaacgctt tgacggcttt gcagcaaaac
    14821 acaatcaccg cgcgccgatt gccgatacgg aaatcaatat tgccaatagt gatatcagct
    14881 tagataacgg ttttgtgcat ttaatggcag aaaaaatcaa tctggacgac agcaaaatca
    14941 atatcacctt caaccaagac aacagcttgg atgaaagtac ccaaatcaac cgcttaggac
    15001 taaacggcaa agtgtcgatg aaaaacagcc atattaacgt agtgggcgat gaaaaagacg
    15061 gtatctcacc ggctcgccat tatgccacga tgttcttagt gggggagctg cttggtgaaa
    15121 agagttctat tttcctcaaa tcccaccaag gtgcagtatt gagaaccgac agcaatgtta
    15181 caattgccgg taaaaacagc aaagacgacc tcaaaattac cgccattacc accggtaaaa
    15241 aaatgggcaa tgaaatcatc ggaccggacg gcttgccaac ctcggaagat aacgatgcca
    15301 acgccagcaa caccgccttt gccatcggct atgaacctaa cgctacggct atcgtcgaaa
    15361 atgcaagcat taccgcatta gcaccaaacg gcggcttggc atacaccggc acgtctaaaa
    15421 cccaaattga cgcgaaggat aatgctacct ttaccttctt tgaaaaacct cgagccggct
    15481 atgacaaatt gcttggcgac atcaaaggta accccgataa attaacccca cgcgatttcg
    15541 cccgtttata cgacaacatt aaaggcatca aagccagcag cctatccgaa caacagcttg
    15601 gcctagcaga caccgcgaag aaaaccagcg aacacacctt agcaaacagc ctagacgtag
    15661 aaaatttggt ttcagtgaca gtgtgtgatg agcagaataa gtgtgaggag cgtatgttag
    15721 gtaacaaaaa taccaatgct aaagttattg tcggagatat caaatttgag tagcgttgaa
    15781 gaaagtggta gaagctcaga cttgatctga taaagcacta taaaaagtga gagtttcccg
    15841 tttagaatat gagtgtcaaa attcaatcta aacaaaaagg aaactctcat gttttattac
    15901 ttacaatccc ctcattaaac acaagaccgg tttattaaat ttagcagaag aaccgggcaa
    15961 tatctctcaa gcctgcaatg tggtgggtat gagcctagat acattctatt gctatcaaca
    16021 agcggttatt tttggcaact aatttgcaaa aaaacaataa aatccgaccg cttgtagagg
    16081 gttaatccgc ccttagtgaa atttcaccga tgccgaaata gcggaggttg tcgccctgtt
    16141 ttagaatgag ttcgccctgc tcgttaatgc cttgcgaaat gccgtggatt tcttcggctt
    16201 cggtaatgag cttaaccgct ttatggcgga aaaggtcgaa actttgccag cgttcggcat
    16261 aatgagcgaa gccgacaagc ggataaattt tgagattttt ttgcaattca taggctaaac
    16321 ggcaaaccaa ttcgttgcgg ttgaaatgat attgcgataa atctgcccac gcttgcgtca
    16381 caatattttc atccactttt gccattccta ggttcagccc aatgccgata accacatcta
    16441 aaccattgcg attggcttgg gtttccaaca aaatgccccc catttttttg ccttggtaat
    16501 aaatgtcatt cggccattta atttgaatat cttttacatt ttgaacagta aggctttccg
    16561 caatgatcaa ggctaccact aaacttaagg gcggtaaatc tgccgcctgc tccatcgggt
    16621 aatgccataa aatcgagaaa tagagatttt cactctcggg cgaataccaa gttcgacctc
    16681 gtctgccacg tccggcagtt tgtttttcag cgaggcaaac cgtgccgttt tccaaacgtt
    16741 ggtgatgagt gagcaaatat tcattggttg aatcaatttc atcgaacaca acggcttgcc
    16801 cgcaaattaa agcattttgg atttgagctt ggttaagttt cattatttcc ccacttagcg
    16861 agtgattttt tcgctatcga cctcaccgtt tttaccgata aaacgcactt caggctgaat
    16921 ttctacccca aatttttctt tcactctctg gcgaacggtt ttggctagag ccaagacatc
    16981 ttgccctgtt gcattttctt tattaatcag cactaaggct tgctgggtat gtaccgccgc
    17041 tccgccaatt tgtaagtctt tcagcccggt ttggtcaatt aaccaacctg cggctaattt
    17101 taccgttcca tcagcttgag ggtagtgagg aatggctggg aatttggctt gtattggtgc
    17161 aaattgttcg tttgaaatca ccggattttt aaaaaagctg ccggcattgc caaattcgtc
    17221 agggtttggc aatttggagg agcgaaccgc acagacttca tcaaaaatct gtattggcgt
    17281 aacggtttcc ggattgagtt gggctaatga tccgtaactt agcaccggtt tccacgcttt
    17341 ggctaacttt aagcctaccg agataatcgc aaattcatca cggtattggt gtttaaacac
    17401 actttcacga tagccaaatt cgcactcgga tttggatagc ttaaagagtt ctccggaacg
    17461 taaatttaac acttccacga aatcacatac ctgttcaaat tcaaccccat aagctcctat
    17521 attttgaatg ggagcagagc tggcgacacc cggaattaac gccagatttt caatgccggc
    17581 aatattgtgt gccaacgtcc atttaaccaa ttcgtgccag ttttccccac cttgaacgtg
    17641 caaatagtgg aaatgttcgt cttcgtggtg cgtaatccct ttgagtttat ttactaacac
    17701 cacaccgtca aaatcctcta aaaacaaaac gttagagcct tgcccgagaa tcaagattgg
    17761 cagttgagcg ttaaaggctt tttgccattc gctcaatagc tgttcgactg tggtaaattc
    17821 aataatttgg gtcgcttttg caggcaaatg gaaagtgtga aaaggggtta agctatgttt
    17881 catcatcaaa ttagttagtt aagaagtgga aataacaagc ggtgatattt ttacaaaatt
    17941 ttgcaattaa gattttgggc aggcacagga tctgccccta cattcagaat taccttgccc
    18001 catataccac aatggttttg ccgtgagcag aaattaagcc ttcgtcttcc aacattttta
    18061 taatgcgtcc aacggtttca cgggaacaac cgaccatttg cccgatttct tggcgggtaa
    18121 ttttgatctg cataccttcc gggtgagtca ttgcatccgg cattttagct aagttgagca
    18181 aggtttgagc aatgcgaccg gcaacgtcta aaaaggcaag attaccgact tggcgtgaag
    18241 tttgacgtaa acggcgagcc atttgtgccg ataaatacat taaaatatcg gggtttaaat
    18301 ggactagttg tttgaatttt ttataggaaa tttccgcaat ttcacaagga cctttagtgc
    18361 ggacgtaagc ggtacgttgt tggacttttt cttcaaataa acttaattca ccgacaaact
    18421 cacctgtgcc taagttagtt aagagcatct ctttggtttc atcatctttg atgtaaaccg
    18481 acactgagcc attcacgatg tagaaaagtg attctgcatc ttcaccggcg tgaatgagtg
    18541 tatatttcgc cggataacga tggatatgac aatgggttaa aaaccactcc accgcagggt
    18601 cgtgaataga gggcggagga gttacatttt ccagaggagc tgttgaaagt gtcatatctt
    18661 gcatagtatt tcctataact cttatactag gttaaatttt tagaatttta acacgaaatt
    18721 aaaggttagc ctaaaaaaga ttttttgtct ttaatatcaa ggctttcgtg taattccgac
    18781 caaacaatca ccactttatc tgccttaagt tgtgtgagta atcgttctct tttttctgcc
    18841 agagaaagtt ccctttcccc gtagtctgtg ccttctcgta aaatgaagga atctaaaata
    18901 ttatgtaagg ttgattcttc gagttcttgc caaggtataa tcataattaa tcaaataaag
    18961 gcttaataaa tgcccactgt ttttcaaaat gttggtgttg gttatagctg aaattagaac
    19021 gaaccaagcg taaaaattgc ccttcgcaga aattcactaa ataaccggct aaggctcttt
    19081 cgtcttcaaa gctcttgcgt tcacgcagtt tactaagttg caaaatattg gcaaattgta
    19141 actccaagtt atcaaagaat tttgccactc gagctttgag caactcctct tcaaacatca
    19201 aggcgtgtcc ggtcaggatg cgggtaacgc ccgggttttt gcgagcaaat tctaaaatca
    19261 catataaaat tgctcgcacc gaattggcac tgctgtcttg gcgtttattg gcgtgaatat
    19321 agctggtgag ggtctgctcg attttctcga ttaacgcttc aaacatcttg gttttacttg
    19381 ggaaatagcg atacaacgcc ccctccgaca cccccaccgc agcggctaaa cgctcggtag
    19441 tgatacgctg cattccctct tcagaattta acaagctgat taacacttcc aacacttgtt
    19501 ggcggcgttc caccactgat tttttcggca ttttaatttt aggttctgtc attgccaatc
    19561 ctattattgc ttacctgaat gcccaaaacc gccttcgcca cggtcggtct gctcaaactc
    19621 ttgcacaatg ttaaaacttg cctgaaccac cggcacaaag actaactgag caatacggtc
    19681 gcccacgttc acggtaaaat cagcttggct acggttccaa agcgacactt tcagctcgcc
    19741 ttggtaatcg ctatcgatca agccgactaa gttgcctagc acgatcccgt ttttatgccc
    19801 taaacctgat cttggtaaga tcaccgctgc caaattcgga tcggcgatat aaattgaaat
    19861 ccccgttggg atcaacaccg tttcacccgc ttttaccgtc agcggctcgt tgagcatcgc
    19921 ccgtagatcc aagcctgccg agccttcggt cgcatacgca ggtaatggaa attcattgcc
    19981 gatacggcta tctaaaattt ttaaatcaat ttgtttcatt ctttttcctt tttgtaaatt
    20041 tttaaaacta tattattcag ttataaaagg ttttaattgt atcgttccaa gtggaagttc
    20101 agtcaatatt tcccagcttg tgttgtggta aaatcgaaat aaacttgcaa cgttttcaac
    20161 gtagcttgaa ttaaatttac cttctaatgg ctttattcct ccagtaaaat gaattatatg
    20221 tggaatataa tttggcgaaa ttttttgctg ggcaatatca tctaataaat gttctgtctg
    20281 ataattatat tttcgatcta actcaatcca attatattga aagcagtaat taagaatatc
    20341 ttgatcccca aatggtaaat tttctttaaa tctctcagta agctgtataa atggttgctt
    20401 actaaaatgc tttgcattta gataaaatac accagcattg aaatagtttt tgtaaatata
    20461 gggatgattt tttagtttgt ttacataagg atcagcaatt gcagcaatac ttccaaaaga
    20521 aaaatctagg tttgtaaatg gatccgtaat atttccatta accacaagat cgcaatctaa
    20581 ataaatccaa tatgggctat cgctgtgttc aaatagttct tcaatataat atcgtaggta
    20641 agttgaatta gtaatatagc cgttggagtt aaaatttgct aaattatttg aatctaaata
    20701 gccaagtctt aatgtggaat atctattttc taaatagggg cgtaatgttt ctatccagtg
    20761 gtgagaaata tcgccagtac tcaatataaa aatagataaa tgattatgat gagctaatag
    20821 tgattttaag gcaatctcta a
//
LOCUS       DANLRK010000020        20505 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.020, whole genome shotgun sequence.
ACCESSION   DANLRK010000020 DANLRK010000000
VERSION     DANLRK010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 20505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20505
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.020"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            7..1470
                     /gene="ubiD"
                     /locus_tag="PXA33_000573"
     CDS             7..1470
                     /gene="ubiD"
                     /locus_tag="PXA33_000573"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HDL5119936.1"
                     /translation="MKYKNLREFLDFLESQGELVRIQQEIDPYLEMTEIADRTLRKGG
                     PALLFENPKGYQMSVLCNLFGTPKRVALGMGQEDTKALRELGKLLAFLKEPEPPKGFK
                     DLIGQLPQWKQILNMPPKVLSKADCQQVVISGDEVDLYKLPIMHCWPGDVAPLVTWGL
                     TITQGPYKKRQNLGIYRQQLIGKNKLIMRWLSHRGGALDFQEWKAENPGKPFLVSVAI
                     GADPATILAAVTPVPDTLSEYAFAGLLRGHKTEVVKSISNELEVPASSEIVLEGYIDL
                     EETALEGPYGDHTGYYNEQEYFPVFTVTHITMRKDAIYHSTYTGRPPDEPAVLGEALN
                     EVFIPILQKQFPEIVDFYLPPEGCSYRLAVVTMKKQYAGHAKRVMMGVWSFLRQFMYT
                     KFVIVCDDDVNARDWKDVIWAITTRCDPARDTTLIEHTPIDYLDFASPIAGLGSKMGI
                     DATNKWAGETTREWGTPIEKDDNVVKRIDEIWDSLGI"
     gene            complement(1555..2379)
                     /locus_tag="PXA33_000574"
     CDS             complement(1555..2379)
                     /locus_tag="PXA33_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL5119937.1"
                     /translation="MDSLTHLFTQFQFRTDLFHLGQLCQIGSFNEENKGYLHFVRQGR
                     FILNQAAERPITIERPAIIFSPTNILHSLHPLDDSGLDIFCINFDFGKGIRNPLTHTL
                     HNIVILELAQYPELGVLADQIFSEVNQKGCGYQAMIHHLCAYFTLKVARQCLEKGYIQ
                     TGLLKGLADKQLGKVLLAIHNAPERDWNVEELANVALMSRSRFAAYFKQTMGVSPMDY
                     LTNWRLAVAQHLLQKGIPVALVAEQVGYSHNAALSRVFMRELGLSPTEWLAQNKES"
     gene            2506..3351
                     /locus_tag="PXA33_000575"
     CDS             2506..3351
                     /locus_tag="PXA33_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDL5119938.1"
                     /translation="MMSQEQFEEINQERRQALNHGSRIAMAASVLGALGIASTANAKQ
                     ASAEKSPYAEPEQYGLKRHGMTIDPKRVALVVVDPQIDFLGPKGVMWGVLGDSIKENN
                     TVANIESLFKAAKAVDMPTFVSPHYYYPTDHKWEFGSPGEHFMHDSGMFARKGQYTME
                     GFENSGADFMPEYKPYIFDGKTVITSPHKIFGPETNDLVLQLRKRKIDQVILAGMAAN
                     LCIDSHLRELIEQGFEVTVVKDATAGPRIPEGDGYLAALTNYRIIANDLWTTEEAVKK
                     MTAKA"
     gene            3808..6750
                     /gene="rne"
                     /locus_tag="PXA33_000576"
     CDS             3808..6750
                     /gene="rne"
                     /locus_tag="PXA33_000576"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HDL5119939.1"
                     /translation="MKRMLINATQKEELRVALVDGQRLFDLDIESPGHEQKKSNIYKG
                     KITRVEPSLEAAFVDYGAERHGFLPLKEISREYFPSDYVFNGRPNIKDIIQEGQEVIV
                     QVSKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDERLELKEALDCLDV
                     PEDVGLIVRTAGVGKSPEELQWDLKVLLHHWEAIKKAAESRPAPFLIHQESDVIVRAI
                     RDYLRRDIGEILIDNKKVFEKAKNHIRLVRPDFINRVKLYEGEVPLFSHYQIESQIES
                     AFQREVRLPSGGSIVIDVTEALTAIDINSSRSTRGGDIEETALNTNLEAADEIARQLR
                     LRDLGGLIVIDFIDMTPVRHQREVENRIREATRQDRARIQFARISRFGLLEMSRQRLS
                     PSLSEASSHICPRCQGTGKVRDNESIALSILRLLEEEAIKENTAQVHTIVPVQIASYL
                     LNEKRKAISSIEKRHDVQIVVVPSESMETPHFSVYRLRENEITPTLSYDLTKHYEARH
                     EEEHPHHHHQEDVLVTRNEPVITVESVLQSSELNLQPSPTPTEPPKPSLLSRLIATIK
                     GWFSEEKVEEKPKPKARNQRERRQRRERPNRNAKAENQERKTPQAQTTPAEEKPMRSE
                     KKARRAVVEEVSGVTEQESRSNSREVQTERRQRRDLRKKVRIETQEVEVENLTPAVEE
                     TVAKQVENVQKNQQIQPLATQEVPVETAPEVETTNEGRENNRQRRLPRHLRVGNQRRR
                     ENQRNQVISPMPLTSAVASPELASGKVWVQVTSTVKVEEKASFLSVDQMLEQQENQLV
                     ETEKKSEEKKLHTVSPLGGFVAQATNRDLVKEKQEQLSKPKEEKVASEDVKPVEVKEH
                     IAPFGSVVAQHSERDLVKEKNERVSKFQSQATVAPAIVEEVTNPIEVPKSTYESDYRF
                     NGRLGTFSRVEHTQAAMTIASAPSDKLEVVEISQWQNSKYYFYGKGFGGYTSAVSQVQ
                     SAPRSTNG"
     gene            6896..7456
                     /locus_tag="PXA33_000577"
     CDS             6896..7456
                     /locus_tag="PXA33_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5119940.1"
                     /translation="MKKSFLSLLVLGTTMALAACDQAKEAAQSTTDKAVEVKEAVAEK
                     AAEVKSATEAKVEELKAAANDAAQATADKTEEAKKAVAEKATELKTTTEAKVEELKAA
                     ANDAAQATTDKTEEAKKAVAEKAAELKATTEAKVEELKAAASDATQATADKAEEAKKA
                     VAEKAAEVKVETETKVEEMKKAIESK"
     gene            complement(7545..7817)
                     /locus_tag="PXA33_000578"
     CDS             complement(7545..7817)
                     /locus_tag="PXA33_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005819506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HDL5119941.1"
                     /translation="MNKTELIDAIAAGANLTKKDAKAALEATLDAISASLKAGDAVQL
                     IGFGTFKVNERKARTGRNPKTGEEIKIAAAKVPAFVSGKALKDLVK"
     gene            complement(7953..8543)
                     /locus_tag="PXA33_000579"
     CDS             complement(7953..8543)
                     /locus_tag="PXA33_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005620054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF416 family protein"
                     /protein_id="HDL5119942.1"
                     /translation="MRNPIHKRMERFEPWQNTTFMACLCERMYPNYQLFCEVTEQPEK
                     AKVFQNILNLVWEYLTIKGAKINFDNQLEKLEEIIPDVNEYAFFGVLPAQEACEALSE
                     LLHSIIAGSTLEQAIRISQISLGTVASYLEMQQDKELNEQELKNSEEIQEELDVQWQI
                     YRLLNECEKRDLDLIFELKDEIRSSGISNIGLNINQ"
     gene            complement(8690..9754)
                     /gene="hemE"
                     /locus_tag="PXA33_000580"
     CDS             complement(8690..9754)
                     /gene="hemE"
                     /locus_tag="PXA33_000580"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HDL5119943.1"
                     /translation="MSELKNDRYLKALLREPVDMTPVWMMRQAGRYLPEYKATRAEAG
                     DFMSLCRNADLACEVTLQPLRRYELDAAILFSDILTVPDAMGLGLSFGAGEGPKFARP
                     VASLNDVNQLPIPDPEDELQYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTKIKKMLYAEPHILHKLLDKLADSVILYLNAQIKAGAQAVMVFDTWGGVLAHED
                     YKAFSLHYMHKIVDGLIRENEGRKVPVTLFTKGGGLWLEAIAETGCDAVGLDWTVDIA
                     DARRRIGDKVALQGNMDPSVLYASPERIEQEVKNILAGFGNGSGHVFNLGHGIHLDVP
                     VESPKVFVDAIHNYSKQYHK"
     gene            complement(9852..10409)
                     /locus_tag="PXA33_000581"
     CDS             complement(9852..10409)
                     /locus_tag="PXA33_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005624207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HDL5119944.1"
                     /translation="MAITDSQQFKQLVSQLQIDDTPAEFHGFLCGLIAGGIQDESWKT
                     LTYQFTNDGHAFSIEPLNTLTDFYQQLSESFSEANTLFSLWLPQNEEDGFALADGISE
                     WTNSFLLGLGVAQPKLQQETDEVGEAIDDLDEIAKLGYSDDDNNEELLEAGEEVLEYL
                     RVLALFLHSHFALGVPKTEEKPPLH"
     gene            10488..10778
                     /locus_tag="PXA33_000582"
     CDS             10488..10778
                     /locus_tag="PXA33_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HDL5119945.1"
                     /translation="MSANNIELQIFGQVLRLQCPVEQQEHLLASAQRLEERVVVLKEQ
                     SGIIQLEKVLAIVALNLNYELEQAQRQNAENKNVLVACIEQLDNSLGKFQSN"
     gene            10883..11074
                     /gene="ssrS"
                     /locus_tag="PXA33_000583"
     ncRNA           10883..11074
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PXA33_000583"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            11076..11654
                     /locus_tag="PXA33_000584"
     CDS             11076..11654
                     /locus_tag="PXA33_000584"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HDL5119946.1"
                     /translation="MNQSSLTDLRSQLRQSMRLKRQSLTAGQQTQAAESIIPQALSLI
                     ESYQASHIAFYLPFNGEISPIPLMEQLLQQGKKLYLPLLHPFTSGQLLFVNYNCKTIL
                     KFNRLGIQEPILDVRNILPLQELEMIFTPLVACDKAGNRLGMGGGFYDRTLAQAPHLI
                     SVGLAHECQQVEQLPIESWDMPLDHIILGAAK"
     gene            11651..12439
                     /locus_tag="PXA33_000585"
     CDS             11651..12439
                     /locus_tag="PXA33_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HDL5119947.1"
                     /translation="MNAKKEKESSGNQSLIRGLTLLELLAKFPNGCPLAHLAELSGLN
                     KSTVHRLLQGLQQEGYVRPAPTAGSYRLTTKCLAIGQRALSSINILHIAAPHLEALNL
                     TLGETVNFAMRENDHAILINKLEATTGMMRTRAYIGQQMQLYCSGMGKLFLAYGSADY
                     IPQYWQTKSDIIQKLTVNTITTIDGMQTELETIRRQGFAMDNEENELGVSCIACPIFD
                     HQQKVHYAISVSLSTARLNQLGRENLLPAIQETAEKISAELGGR"
     gene            12533..14047
                     /gene="putP"
                     /locus_tag="PXA33_000586"
     CDS             12533..14047
                     /gene="putP"
                     /locus_tag="PXA33_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HDL5119948.1"
                     /translation="MFGLDPTTIMFVVYILGMIAIGFIAYRVTNNLSDYILGGRRLGS
                     FVTALSAGASDMSGWLLMGLPGAVYAGGLIEGWIAIGLTIGAYLNWKLVAGRLRSHTE
                     HSGDALTLPEYFHNRFGDKTRLIKILSAVIFLLFFAIYCASGVVAGARVFENLFGLPY
                     HQAIWYGAVATILYTFIGGFLAVSWTDTIQASLMVFALILTPFFVMYNLGGFDEMQAL
                     IDRAGEMAQKDYNDIFTGTSVLGIISLMAWGLGYFGQPHIVVRFMAAESVKSLENARR
                     ISMTWMIICLLGAIGIGYFGMAYFFGNPEQVGNISKNPEQIFIELAKLLFNPWVAGIL
                     LSAILAAVMSTLSAQLLICSSAITEDLYKGILRPQASDKELVWLSRLMVLAVAALAIY
                     IAQDPDSKVFGLVRNAWAGFGSAFGPVVLLSLFWKRMNGFGAIAGMLTGALIVHFWGD
                     ITLKLALPEIYSMIPGFIAATLMIIVVSLITPMPSKAVLDTFDRANKAYLAEVK"
     gene            14199..15365
                     /locus_tag="PXA33_000587"
     CDS             14199..15365
                     /locus_tag="PXA33_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL5119949.1"
                     /translation="MTNLLLAIIYLSFISLGLPDAILGSAWPSMHQQLAVPLSYAGVV
                     SMIMSIGTITSSLMSDKLTYHLGTGKVMVISVALTALALWGFSISDAFWILCLWAIPY
                     GLGAGAVDAALNNYVAVHYASRHMSWLHCMWGVGASIGPHILGYALLQGRGWEAGYFT
                     ISMLQIGLTLMLFCSLPFWKLRKQITAEINHSAKPMSLKETLKIPGVKYGILAFFGYC
                     GVEQTAGLWASSYFVMVKDVGLEQAAAFGSIFFIGITVGRGVSGFLTLKFSNRQMIKL
                     GSALILVGIAILCLPLGKYVTLASLIIIGLGCAPIFPCLIHSTPAFFGEDKSQSVIGV
                     QMASAYIGVLLLPPIFGFIANAISIQLLPLYLLLMFAIMAYAYRNLVRETSPSP"
     gene            complement(15610..16482)
                     /gene="rsmI"
                     /locus_tag="PXA33_000588"
     CDS             complement(15610..16482)
                     /gene="rsmI"
                     /locus_tag="PXA33_000588"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HDL5119950.1"
                     /translation="MKNRQNSSGINPNYGILYIVATPIGNLGDITQRALDTLSSVDLV
                     AAEDTRHSGLLLSHYGIKKPFFALHDHNEQQKAVVLVEKLAKGENIALISDAGTPLIS
                     DPGFHLVRHCRQAGVKVVPIPGACAAVTALCASGIASDRFCFEGFLPAKTKSRCDKLT
                     ELENEPRTLIFYESTHRILDTLADMQTVFGEDRYVVMAREITKTWETIYGDQLGNLIE
                     WLNEDSNRIKGEIVLVVEGKPQSSEEAFSSQAIKLLTLLCQELPLKKAAAIVAETFGY
                     KKNALYQYGLEHFE"
     gene            16556..18277
                     /locus_tag="PXA33_000589"
     CDS             16556..18277
                     /locus_tag="PXA33_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HDL5119951.1"
                     /translation="MATILNHTMKKALVPTAIALFISACTSVNPVTESIKNEAYSSSE
                     FYINKADQTKEAEDKISYQLLAVRKLIDENKEYEAQNTFSEILTAEMNEVQKLEYALV
                     SAQLAALQGKNEQATSQLKAIQDPLLSSAQRLRYIQTQARIAANQKDVIGIVRARSQL
                     NNFYKMNRERQENNDIIWQTLRDANRGMLEKTVPEAGEMELAGWLALINIYNQNVTTP
                     AQMPQAINNWKAQYPSHSAVAVMPTELQGVSNFQQTQLNSVALLLPLSGDAKILGDII
                     KRGFNDAKADDSTAVQTFDTDSSDVNSLISQAKQQGANVIVGPLLKSRVDEMLLSPEI
                     QNVNILALNATENVRNIAQVCYYGLSPESEAQSGAEKIYQDGNSVAIVAAPQDDYGNR
                     SAEAFAKRWRQLTNSDADVRYYNQPLDAVAAIQNAGVSKAALYILGNAEQVLEIKQGI
                     DSSTLKDRLAIYTSSRSNSPNNGIDFYTSMEGVKFSEVPLLADQSSSEYKKAENLANS
                     DFSMMRLYAMGADAWSLATKFNEFRHIPGYKISGLTGELKAGQHCNIERSLSWMQYRN
                     GAIQQAY"
     gene            18252..18623
                     /locus_tag="PXA33_000590"
     CDS             18252..18623
                     /locus_tag="PXA33_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HDL5119952.1"
                     /translation="MELFNKLINKREQGSFYEQKARVFLEQQGLRFVAANQAFKGGEL
                     DPIMQDGQTVVFVEVRQRKSNRFGSAVESIDYRKQQKWQNAANMWLLKQNQSLETANC
                     RFDVVAFEGNQTPVWIPNFLG"
     gene            18629..19213
                     /locus_tag="PXA33_000591"
     CDS             18629..19213
                     /locus_tag="PXA33_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL5119953.1"
                     /translation="MLNKIQDRFTESIQTQISASELLPAKLSEAANRIVACLLRGDKV
                     IVCGHGRSYGNAELLVSHLLHRYELARPSFAAVQLQLSGVLAGVVAQDNELEAVYKKQ
                     LQAVAKEGDLFIVFSPVGNEECILQAIHTAKNQHLDLDIIALTGSNNDHTLGLLDESD
                     IEISAPSTNQLRVIESHHFYVNLLCELIDHLLFS"
     gene            19223..19813
                     /gene="yraP"
                     /locus_tag="PXA33_000592"
     CDS             19223..19813
                     /gene="yraP"
                     /locus_tag="PXA33_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HDL5119954.1"
                     /translation="MNKLANKLLKGSLLVLGFISLQGCITTAVVTSAAVATKVATDPR
                     TTGTQVDDEILEEKVAYNINKDEQIKQEARINVVAYNGKVLLIGQAPSMDVVENAKNL
                     AAGAEGVTEIYNEIRQGEKIGFGQITQDSWITTQVKSKLLVNSEVKATEVKVVTENGE
                     VFLMGKVSQNQADAAAEAARNVGGVTKVVKVFSYAQ"
     gene            20103..20294
                     /locus_tag="PXA33_000593"
     CDS             20103..20294
                     /locus_tag="PXA33_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5119955.1"
                     /translation="MLFKEQGYDEFLAEQIKAGQEAIARGETFTKEQINNELNHLFAD
                     MQKRQQLRENENLFEVNYA"
BASE COUNT         6091 a         4218 c         4490 g         5706 t
ORIGIN      
        1 accgctatga agtataaaaa tctccgtgaa tttctcgatt tccttgagtc tcagggtgaa
       61 ttagtccgta ttcagcaaga aatcgatcct tatttagaaa tgacagaaat cgccgaccgc
      121 accttacgca aaggcgggcc ggcattactg tttgaaaacc cgaaaggcta tcaaatgtcg
      181 gtgttgtgta atttattcgg cacgcccaaa cgggtagctc tgggaatggg gcaagaagat
      241 accaaagctc tgcgtgagct gggcaagtta cttgcctttt taaaagagcc tgaaccaccg
      301 aaagggttta aagatttgat cgggcaactg ccacaatgga agcaaatttt aaatatgccg
      361 cctaaggtac tgagcaaagc cgattgtcag caagtggtga tttcgggtga tgaggttgat
      421 ctctacaaat taccgattat gcactgttgg ccgggcgatg tcgctccact ggtcacttgg
      481 gggctgacga ttacgcaagg gccttacaaa aaacgccaga atttaggcat ttatcgtcag
      541 caacttatcg gcaaaaacaa actgattatg cgttggcttt cccaccgtgg cggtgcgtta
      601 gacttccaag agtggaaagc cgaaaacccg ggtaaaccgt ttctggtgtc ggttgcgatt
      661 ggtgcagatc cagccactat tttagcggca gtcacccctg tgccggatac gctttcggaa
      721 tatgcgttcg cagggctgtt gcgagggcat aaaaccgaag tggtcaaatc tatttccaat
      781 gagttagaag tgccggcaag tagcgaaatt gtgctggagg gctatatcga tctggaagaa
      841 accgcattgg aaggccctta tggcgaccac accggctact acaacgagca agagtatttt
      901 ccggtattta ccgttaccca tattacgatg cgaaaagatg cgatttatca ttctacctac
      961 actggcagac cacctgatga accggcagta ttaggtgaag cattgaatga agtctttatt
     1021 ccgattttgc aaaaacagtt tccggaaatt gtcgatttct acttgccacc tgaaggctgt
     1081 tcttaccgtt tagcggtagt gacgatgaaa aaacaatatg ccggacacgc taaacgggtg
     1141 atgatgggag tttggtcgtt cctgcgtcaa tttatgtaca ccaagtttgt gattgtgtgc
     1201 gatgacgatg tgaacgctcg agattggaaa gatgtaattt gggcgatcac cacccgttgt
     1261 gacccggctc gagatacgac actgattgaa cacacgccga tagattatct cgattttgcc
     1321 tcgccaattg cagggttagg ctcaaaaatg gggattgatg ccaccaataa atgggcgggt
     1381 gaaaccaccc gagaatgggg aacacccatc gaaaaagatg acaatgtggt gaaacgcatt
     1441 gatgagattt gggattctct tggcatttaa ggcaacattt aaccgcttgt gcaacataac
     1501 aagcggttaa attttttgac atttttacca ttcggctatt tgtttctcat caggttacga
     1561 ctctttattt tgagccagcc actcggtagg cgaaagccct aattcccgca taaagacacg
     1621 gctgagagca gcattatggc tataaccgac ttgctctgcc accaatgcta ctggtatccc
     1681 tttttgtaat aaatgctggg caaccgctag tcgccagtta gtgagataat ccatcggtga
     1741 aacccccatc gtttgtttaa aataagcagc aaagcgtgag cgagacatta atgccacatt
     1801 tgccaactcc tctacattcc aatctcgctc tggtgcattg tgaatagcta acagcacttt
     1861 ccccagttgc ttatctgcca agccttttaa caaacctgtt tgaatatacc ctttttccaa
     1921 acattggcgg gcaactttca aggtaaaata ggcacataaa tgatgaatca ttgcctgata
     1981 accacaccct ttctgattca cttcactaaa aatttgatcg gctaataccc ccaattccgg
     2041 atactgtgcc aactctaaga tcacaatatt gtgcaaggta tgagttaatg ggttgcgaat
     2101 ccctttgcca aaatcaaaat taatacaaaa tatatctaac ccactatcat ctaacgggtg
     2161 caaactatgc aaaatattgg ttggtgaaaa aataatcgct gggcgttcaa tcgtaatcgg
     2221 gcgttcggca gcctgattta aaataaaacg tccctgacga acgaaatgta aatagccttt
     2281 attctcttca ttaaaactcc cgatttggca taactgacct agatgaaata aatccgtgcg
     2341 aaactgaaat tgggtaaaaa gatgagtcaa actatccaca ttaagctcct tgggtaaaga
     2401 attgagacga acggttaaaa caggagaaat gcgatcacta taatagcaac ccgttcggta
     2461 acgcaagtta tcattacaat ttgtatatct attaatgagg ataaaatgat gtcacaggaa
     2521 caatttgaag aaatcaacca agaacgccgt caagccctta accacggctc tcgtattgca
     2581 atggcagcca gtgtattagg tgcattaggt attgcatcaa cagcaaatgc aaaacaagcc
     2641 agtgcagaaa aatcacctta tgccgagccg gaacaatacg gtttaaaacg ccatggaatg
     2701 accattgatc caaaacgtgt ggcattagtg gtagttgatc cacaaattga cttcctaggc
     2761 ccgaaaggcg tgatgtgggg cgtgttaggt gactcaatta aagaaaacaa cactgtagct
     2821 aacattgaaa gcttatttaa agcagcaaaa gcagtcgata tgccaacctt tgtttcgcca
     2881 cactactatt acccgactga tcacaaatgg gaatttggtt caccgggtga acactttatg
     2941 cacgacagcg gaatgtttgc gagaaaaggt caatacacca tggaaggctt tgaaaattca
     3001 ggtgcagact ttatgcctga gtacaaacca tacatttttg atggcaaaac agtgattacc
     3061 tctccacaca aaatctttgg accggaaacc aatgacttag tgttgcaatt acgcaaacgt
     3121 aaaatcgacc aagtaatttt agccggtatg gctgccaact tatgtattga ttcacacttg
     3181 cgtgaattaa tcgaacaagg ttttgaagta acagtggtga aagatgcaac cgcagggcct
     3241 cgtattcctg aaggtgacgg ctaccttgca gcattaacca actaccgtat tatcgcaaat
     3301 gacctctgga ccactgaaga agcagtgaaa aaaatgacgg caaaagcata atattcgctt
     3361 tacaagcggt taaatttagc tctttttttg caaatttaac cgcttggatt tcacgcttat
     3421 ctccagcact cacactaaat ctcattattt ttccttatca ccccattcat tttcattata
     3481 ttcatctcca caacaaataa cagctaccat acaacaatta ccttgccttt ttcagggtta
     3541 aaatgcgata atgactcgtt atttttggct tgaaaaagca atagaaaatt gcaaaaaata
     3601 aagcaaaaat aaccgcttgt aatagagata aacaagcctt aagtgattga ttttgcagtt
     3661 tctttaagac aagacaaagt aaacgtaaga caaccatcaa tgcactatcg ggaatgaaga
     3721 cagtcgtgag atagacatta aatccattcc cttattaatg ggtcaaaatg accgtgtaag
     3781 acaaaaacaa aataatgaga aaacacaatg aaaagaatgt taatcaatgc aactcaaaaa
     3841 gaagagttgc gtgttgccct cgttgatggg caacgcttat ttgacttgga cattgaaagc
     3901 ccgggtcacg aacagaaaaa atccaatatc tacaagggta aaattacccg tgtcgaacca
     3961 agccttgaag ctgcattcgt agattacggt gcagaacgcc acggcttcct acctttaaaa
     4021 gaaatttccc gagaatactt cccgtcagac tatgtgttta acggtcgccc gaatatcaaa
     4081 gatattattc aagaagggca agaagtgatt gttcaagtca gcaaagaaga gcgtggtaac
     4141 aaaggagcag ccttaaccac ctttatttcg cttgcgggca gttacttagt cttaatgccg
     4201 aacaaccctc gtgccggtgg tatttcccgc cgtattgaag gtgatgagcg tttagaactc
     4261 aaagaggctt tagactgctt agatgtaccg gaagacgtgg gcttaatcgt tcgtaccgcc
     4321 ggtgtcggca aatcgccgga agagttacaa tgggacttaa aagtcttgtt acaccactgg
     4381 gaagcgatta aaaaagcggc ggaatctcgc ccagctccgt tcttaattca ccaagaaagt
     4441 gacgtgattg tgcgtgcaat tcgtgactat ctacgccgtg atattggcga aatcttaatc
     4501 gacaacaaaa aagtgttcga gaaagccaaa aaccacattc gccttgttcg cccggatttc
     4561 atcaaccgtg tgaaattata cgaaggcgaa gtaccactat tcagccacta tcaaattgaa
     4621 tcgcaaatcg aatcggcatt ccaacgtgaa gtgcgtttac cttccggtgg ctctatcgtt
     4681 attgatgtaa cagaagcctt aacagcaatt gatattaact cctcacgttc aacccgtggc
     4741 ggcgatattg aagaaaccgc tcttaacacc aacttagaag cggcagatga aattgcccgt
     4801 cagttacgtt tgcgtgactt aggtggtttg attgtgatcg actttatcga tatgacaccg
     4861 gttcgccacc aacgtgaagt agaaaaccgt attcgtgagg cgacacgcca agaccgtgcg
     4921 agaatccaat ttgcccgtat ttcccgtttc ggtttgttag aaatgagccg tcagcgttta
     4981 agcccatcat taagtgaggc ttcatcgcat atctgccctc gctgtcaggg aactggtaaa
     5041 gtacgtgata atgaatcaat cgcactgtca atcttacgtt tattagaaga agaggcaatc
     5101 aaagaaaaca ctgcacaggt tcacaccatt gtgccggtgc aaatcgccag ctacttactc
     5161 aacgaaaaac gtaaagcgat ttccagcatt gaaaaacgcc acgatgtaca aattgttgta
     5221 gtaccgtctg aaagtatgga aacgccgcat ttcagcgtat atcgcctgcg tgaaaatgaa
     5281 attactccaa cgttaagcta cgatttaacc aagcattacg aagctcgtca tgaagaagaa
     5341 cacccacatc atcaccatca agaagatgtg ctggttaccc gtaacgagcc ggtgattacg
     5401 gtagagtcag tgttacaaag ctcggagctg aatctccaac cttcgccaac accaaccgaa
     5461 ccaccaaaac catcgctgtt aagccgctta attgcgacca ttaaaggctg gtttagtgaa
     5521 gaaaaagtgg aagaaaaacc gaagccgaaa gcacgcaatc agcgtgagcg tcgccaacgc
     5581 cgtgaacgcc cgaaccgtaa tgctaaagcg gaaaatcaag agcgtaaaac tccgcaagct
     5641 caaactactc cagcggaaga aaaaccgatg cgtagcgaga aaaaagcacg ccgagcggta
     5701 gtggaagaag tttccggtgt aaccgagcaa gagagtcgta gcaactcccg tgaggttcaa
     5761 actgaacgtc gccaacgccg tgatctacgt aaaaaagtgc gtatcgaaac acaagaagta
     5821 gaagtggaaa atctaacacc tgctgttgag gaaacggtgg caaaacaagt tgaaaatgtg
     5881 caaaaaaatc aacaaattca accgcttgca acccaagaag tacctgttga aacagctcct
     5941 gaggtagaaa caacgaacga aggacgtgaa aataaccgcc aacgccgttt acctcgccat
     6001 ttgcgtgtgg gcaatcagcg tcgtcgtgaa aatcaacgca atcaggttat ttctcctatg
     6061 cctctaactt cagctgttgc ttcgcctgag cttgcgagcg gtaaagtttg ggttcaggtt
     6121 acctctactg tcaaagtaga ggaaaaagca tcattcttat ctgttgatca aatgcttgaa
     6181 cagcaagaaa atcagcttgt tgagacagag aaaaaatcag aagagaagaa gcttcataca
     6241 gttagtccgc taggtggctt tgtagcacaa gcaactaacc gtgatttggt taaggagaag
     6301 caagaacagc tttccaaacc gaaagaagaa aaagttgctt ctgaagacgt aaaaccagta
     6361 gaggtaaaag agcatattgc tccatttggt agtgttgttg ctcagcattc tgaacgtgat
     6421 ttagttaaag agaaaaatga gcgagtcagt aaattccaat cgcaagcaac ggttgcacca
     6481 gcgattgtgg aagaggttac taacccgatt gaagtgccaa aatcaactta cgaaagcgat
     6541 tatcgattta acggcagatt aggtacattc tctcgtgttg agcataccca agccgctatg
     6601 accattgctt ctgcaccaag tgacaagctt gaagtagtcg aaattagcca atggcaaaac
     6661 tctaaatact atttctatgg caaagggttt ggcggatata ccagcgcagt gagccaagtg
     6721 cagtcagcgc ctcgttcaac caatggttaa gtacaattaa ttggtttaaa tgcctattag
     6781 ttataactaa taggcatttt tttatcaaag tatgaacttt ctatcaattt ttctgcctaa
     6841 gtaaacgcac tttattacaa agtgcgttat ttttagttta tttaaggaac tcggtatgaa
     6901 aaaatcattt ttatcattat tagtgttagg tacaactatg gcacttgctg cttgtgatca
     6961 ggcaaaagaa gccgcgcaat caactacaga caaagcggtt gaagtaaaag aagctgtagc
     7021 tgaaaaagct gcagaagtta aatcggctac tgaagcgaag gttgaagagt tgaaagcggc
     7081 agcaaatgat gcagctcaag caacagcaga taaaacagaa gaggcaaaaa aggcagtggc
     7141 agagaaagcc actgagttaa aaaccacaac tgaagctaaa gtggaagaac taaaagcagc
     7201 agcaaatgat gcagctcaag caacaacaga taaaacagaa gaggcaaaaa aggcagtagc
     7261 agagaaagct gctgagttaa aagccacaac tgaagctaaa gtggaagaac taaaagcagc
     7321 agcaagtgat gcgactcaag caacagcaga taaagcagaa gaagctaaaa aggcagtagc
     7381 tgagaaggca gcagaagtga aagttgagac agaaacaaaa gttgaagaaa tgaaaaaagc
     7441 gattgaaagc aaatagtgct ttttagtttc tctgtagcta tcgaaacacg ctaaaaataa
     7501 agcctggatt ataatccagg ctttattttt atatctttaa agaattattt tactaaatct
     7561 tttaatgctt tacctgaaac gaatgcaggt actttagctg ctgcaatttt gatctcttca
     7621 ccagtttttg ggttacgacc agtacgtgct ttacgctcat ttactttaaa agtaccgaag
     7681 ccgattaatt gaacagcgtc gcctgctttt aagcttgcag agattgcatc aagagttgct
     7741 tctaatgctg ctttagcatc tttcttggtt aagtttgcac cagcggcgat agcatcgatt
     7801 aactcagttt tgttcatagt tttatcctct aagatatgtg tttttaataa atgacgtaaa
     7861 acgtctgaga agttattgaa accttgcgat agcttaattc aaagttaaga tttctcaaag
     7921 tattattaag caagattttg caaatatcaa tgctattggt taatatttaa cccgatatta
     7981 ctaatcccgc tgcttctgat ttcatcttta agttcaaaaa tgagatcaag atcacgtttt
     8041 tcacattcgt ttagcaagcg gtatatttgc cactgtacgt ccagttcttc ttgaatttct
     8101 tcagaattct ttagctcttg ctcgttcagt tctttatctt gttgcatttc caaatagctg
     8161 gcaactgtgc ctaatgaaat ttggctgatg cggattgctt gttcaagggt tgaaccagcg
     8221 ataatactgt gaagaagttc agaaagggct tcgcaagctt cctgtgcagg taagacacca
     8281 aaaaacgcat attcgttcac atcaggaatg atttcttcta atttttccag ttggttatcg
     8341 aaattaattt ttgccccttt tatggttaaa tattcccaga ctaaatttaa aatattttgg
     8401 aataccttag ctttttcagg ctgttcggtt acttcacaaa aaagctgata gttggggtac
     8461 attctttcgc ataggcacgc cataaatgtg gtgttttgcc aaggctcgaa acgttccatg
     8521 cgtttatgaa tcgggtttct cattgtattt ccttctttat ttttcagaga gaagggaacg
     8581 attttgcaaa attctgtata aaaatgaccg cttgcccctc tccctgattt ttcttctgaa
     8641 caaagaaaaa tctgtccctc tcccacaagg agagagggca ttttatccat tatttatgat
     8701 actgcttcga gtagttgtga atcgcatcaa caaacacttt cgggctttcc accggcacat
     8761 ctaaatgaat gccgtgtcca agattaaaga cgtgtccgct gccgttgccg aagccggcaa
     8821 gaatgttttt tacttcttgt tcgattcgtt cagggcttgc atacagcacg cttggatcca
     8881 tattgccttg caacgcaact ttgtcgccaa tacggcggcg agcatcggca atatcgactg
     8941 tccaatctag cccaacggca tcgcagcccg tttctgcaat cgcttctaac cataagcctc
     9001 cgcctttggt gaatagggta acaggcactt tacgtccttc attttctcga attaagccgt
     9061 ccacaatttt gtgcatatag tgtaatgaga aggctttgta atcctcgtga gccaatacac
     9121 cgccccaagt gtcgaatacc atcaccgctt gagcaccggc tttgatttgg gcatttaagt
     9181 ataagatgac actgtcagct aacttgtcca gcaatttgtg tagaatgtga ggctcagcgt
     9241 aaagcatttt tttgattttg gtaaatgctt tgcttgaacc accttccacc atataggtgg
     9301 cgagtgtcca agggctgcca gaaaaaccaa ttagtggcac ttcgcctttc aattctcggc
     9361 gaatggtacg caccgcattc attacatatt gcaactcgtc ttccggatca ggaatcggta
     9421 gctgattgac atcattgagg cttgccaccg ggcgggcaaa tttagggcct tcacctgcac
     9481 caaaacttaa acctaatccc attgcatcag gcacagttaa aatatcggaa aataggatag
     9541 ctgcatcgag ttcataacgg cgtagcggtt gtaaggtgac ttcacacgct aaatcagcgt
     9601 tacggcagag tgacataaaa tcgccggctt ctgctcgggt ggctttatat tccggcaaat
     9661 aacgcccggc ttgacgcatc atccagaccg gggtcatatc cacaggttca cgcaataacg
     9721 cttttaaata gcgatcgttt tttaattctg acattggttt ttcctttgat tttggtaggg
     9781 gcgaaatatt tttcgccccg cataatatat ttgcaaaata gagatgaaat tgaaccgctt
     9841 gtatttattc attagtgtaa tggtggcttt tcttcggttt tcggcacgcc taaagcaaaa
     9901 tggctgtgca agaataaggc taaaactcgc agatattcca acacttcttc gccagcctcc
     9961 agtagttcct cgttattatc atcgtcactg tagcctagtt tggcgatttc atctaaatca
    10021 tcgattgcct cgccaacctc atcggtttct tgttgtaatt taggttgagc aacgccaagt
    10081 ccaagtaaga aactgtttgt ccattcggag ataccatcag ctaaggcaaa accatcttct
    10141 tcgttttgtg gcaaccaaag gctaaacagc gtgttggctt ctgaaaagct ttcacttaat
    10201 tgctgataaa aatcggttaa agtgtttaac ggctcgatgg aaaatgcgtg tccgtcattg
    10261 gtaaattggt aggtgagggt tttccaagac tcatcttgaa taccgccggc aattaaaccg
    10321 cataaaaaac cgtgaaactc tgccggtgta tcatcaattt gtaattgtga taccagttgt
    10381 ttaaattgtt gggaatctgt gatagccata atgggattat ttgtgatttt gctaaagaat
    10441 ggcataatat agcaaaaatt ttgcgattaa gtgaggattt cgtatttatg tcggcaaata
    10501 atatcgaact acagattttt gggcaggttt tacgcttgca atgccctgtt gaacagcaag
    10561 agcatttact ggcttcagca cagcgtttgg aagagcgtgt ggtggtattg aaagagcagt
    10621 ccggcattat tcagcttgaa aaagtgttag cgattgtggc actgaattta aattatgaac
    10681 tggagcaagc ccaacgccaa aatgcagaaa ataaaaatgt gttggtggct tgtattgagc
    10741 agttagataa ttcgcttggc aaatttcaat ccaattaggt aatttttaat cgaattgatt
    10801 aggaatagag ccaaaaagat tgagaatttg cattttgtgg gattaaatac taggaaaaat
    10861 catcaaattc cgttactata agtcttgcct ggggtgtgcg ttacgagtta cgtccctgag
    10921 ccgataattt gaaactttga gttggacatc taacgctggt gagcaagctt accctaattt
    10981 aattaggagt aagaagccta aaagcagtta agtttgactc ccttgaaccg actggttcaa
    11041 ggaccattac tggtagcggc atctcgggtc tttatatgaa tcaatcatct ttaaccgatc
    11101 ttcgtagcca attacggcaa tcaatgcggc ttaaacggca aagtttaact gccggtcagc
    11161 aaactcaagc agccgaatcc attattcctc aagctctttc attgattgaa tcttaccaag
    11221 cctcacatat tgccttttat ttgcctttta acggtgaaat ttctcctatt cctttaatgg
    11281 aacaattact tcaacagggc aaaaagctgt atctgccgct gttgcaccct tttacaagcg
    11341 gtcaattatt gtttgtaaat tacaattgca aaactattct aaaatttaac cgcttgggga
    11401 ttcaagagcc gatattagat gttagaaata tccttccgtt gcaagaatta gagatgattt
    11461 ttaccccact tgttgcttgt gataaagccg gaaatcggct gggaatggga ggtggctttt
    11521 atgatagaac ccttgctcaa gcacctcatc tgattagtgt cggtttggca cacgaatgtc
    11581 agcaagtcga acaattaccg attgagagtt gggatatgcc gttagatcat attattttgg
    11641 gagcagccaa atgaatgcaa agaaagaaaa ggaatcgagt ggaaatcaaa gtttaattcg
    11701 tggcttaaca ctattggaat tgttagcgaa attcccaaac ggctgtccgc ttgcccattt
    11761 agccgagctt tcggggctga acaaaagcac ggttcaccgt ctgctacaag gcttacaaca
    11821 agagggctac gtccgccctg caccgactgc gggcagttat cgtttaacca ccaaatgttt
    11881 ggcaatcggg cagcgagcct taagttcgat taacattttg catattgccg ccccacattt
    11941 agaagcccta aatttaacct taggcgagac ggtaaatttt gcgatgcggg aaaacgatca
    12001 cgctatttta atcaataaat tagaagctac caccggaatg atgcgtaccc gagcctatat
    12061 cgggcaacaa atgcaactct attgctcggg aatgggaaaa ctgtttcttg cttatggttc
    12121 ggcagattac attccacaat attggcagac taaatcggac atcattcaaa aactgacggt
    12181 caataccatc accacgattg acggaatgca aaccgagctg gaaaccattc gccgtcaagg
    12241 ttttgcaatg gataacgaag aaaatgaatt aggggtttct tgtattgctt gcccgatttt
    12301 tgatcaccaa caaaaagtcc attatgcgat ttctgtttcg ctttcaactg ctcgtttaaa
    12361 tcagctgggg cgggaaaatc tactaccggc tattcaagaa acagcagaaa aaatttcagc
    12421 tgagctgggc ggacggtaat tttgtgttgc cattttgatt atttcaggta atcttttacc
    12481 ctttactcac tttaatcttt atacaataag aaatcttgag gattcattaa taatgtttgg
    12541 tcttgatcca acaacaatta tgtttgttgt ctatattcta ggaatgattg ccatcggctt
    12601 tattgcctat cgtgttacca ataacctttc agactatatt ttaggcggtc gccgtttagg
    12661 tagctttgtg accgcacttt ctgccggtgc atcagatatg tccggctggt tgctgatggg
    12721 attacccgga gccgtttatg caggcggttt gatcgaaggc tggatcgcaa tcggtttaac
    12781 tatcggtgct tatctaaact ggaaattagt cgcaggcaga ttacgctcgc atacagaaca
    12841 cagtggcgat gccttaacct tacccgaata ttttcataac cgttttggcg ataaaacccg
    12901 tttaattaag attttatcag cggtcatttt tttactcttt tttgcaattt actgtgcatc
    12961 aggcgttgtg gccggtgcga gggtatttga aaacttattt ggcttacctt accaccaagc
    13021 aatttggtat ggggctgttg ctaccattct ttacaccttt attggtggtt tcttagcagt
    13081 aagttggacg gatactattc aagcttcatt aatggttttt gccctgattc tcaccccgtt
    13141 ttttgtgatg tataacttag gtggttttga tgaaatgcaa gccttaatcg accgtgcagg
    13201 cgagatggca caaaaagact acaacgacat tttcaccggc accagcgtat taggcattat
    13261 cagcttaatg gcgtgggggc tgggttattt cggacaaccg catattgtgg tgcgttttat
    13321 ggcggcagaa agtgtgaagt ctcttgaaaa tgcacgccgt atcagtatga cttggatgat
    13381 tatctgctta ctgggggcaa taggcattgg ttattttggt atggcatatt tctttggcaa
    13441 cccggagcaa gtcggcaata tcagcaaaaa tccggaacaa attttcattg aattagctaa
    13501 attgttattt aacccttggg tagccggcat tttattatct gccattcttg ctgcggtaat
    13561 gagtaccctt tccgctcaat tactgatttg ttcaagtgcg attaccgaag atttatacaa
    13621 aggcatttta cgccctcaag cctccgataa agaactggtt tggttaagcc gcttgatggt
    13681 gcttgcggtg gcagcattag ctatttatat tgcccaagac ccggacagca aagtatttgg
    13741 cttagtgcgt aatgcgtggg caggtttcgg ctcggcattt gggcctgtgg tattgctttc
    13801 actattctgg aaacgtatga acggttttgg ggcgattgcc ggaatgctga ccggtgcgtt
    13861 aatcgtgcat ttttggggcg atattacact taagctcgcc ttaccggaaa tttactcgat
    13921 gatcccgggc tttattgctg ctaccctaat gatcattgtg gtatcactta tcacgccaat
    13981 gccaagcaaa gccgtattag acacctttga ccgtgctaat aaggcctatt tggcagaagt
    14041 gaaataataa aatttggctt aaggtctcct taagccattt ttttacaaaa aatttatcaa
    14101 aaattattca caatccttgt aaggattgtt cacagcttcg ctcccgttgg cagagccaac
    14161 gttcaaaaag cgatgctttt tgtgaccgat tgtaagctat gactaacctc ctactcgcta
    14221 ttatttatct ctcttttatc agccttgggt tgcctgatgc aattttaggc tcggcgtggc
    14281 cgtcaatgca ccaacagctt gctgttccgc tttcctatgc cggtgtggtc tcgatgatta
    14341 tgtcgattgg cactattacc tctagcttaa tgtcggacaa actgacctac catctcggca
    14401 caggcaaagt aatggtcatc agcgttgcct taacggcttt agcattgtgg ggcttttcaa
    14461 tcagtgatgc tttctggatc ttatgtttat gggcaattcc ttacggctta ggggctggag
    14521 cggttgatgc ggcgttgaat aactatgtag cagttcacta cgcaagccgc catatgagct
    14581 ggctgcattg tatgtggggc gttggagctt ctatcgggcc gcacatttta ggctacgcct
    14641 tattgcaagg gcgaggctgg gaggcaggct atttcaccat ttcaatgtta caaatcgggc
    14701 taacgctaat gctgttttgc tcactgcctt tttggaaatt acgcaagcag attaccgctg
    14761 aaatcaacca ttcggcaaaa ccgatgagct taaaagaaac gctaaaaatt ccgggcgtga
    14821 aatatggcat tttagctttt tttggctatt gtggcgtaga acaaaccgcc ggactgtggg
    14881 cgagtagcta ttttgtaatg gttaaagatg tcggcttaga acaagcggct gcgttcggca
    14941 gtattttctt tatcggcatt accgttggca gaggcgtaag tggcttcttg acgcttaaat
    15001 ttagcaacag acaaatgatt aaacttggtt cagccttgat cttagtcggc attgcgatac
    15061 tgtgcttgcc gttaggcaaa tatgttacgc tggcaagttt aattattatc gggctaggtt
    15121 gtgccccgat tttcccgtgc ttaattcatt cgacccctgc gtttttcggc gaggacaagt
    15181 ctcaatcggt gatcggcgtg caaatggcaa gtgcctatat cggcgtcctg ttattgccac
    15241 cgattttcgg ctttattgct aatgctatca gcattcaatt attaccgctt tacttgctgt
    15301 tgatgtttgc gattatggca tatgcttatc ggaatttagt gcgagaaact tccccctctc
    15361 cctgattttt cttctgaacg aagaagaatc tgtccctctc ccacgagtgg agaggggtcg
    15421 acaagcgaat aatttagctc tctcccctta tgggacaaat gtttacattt gaacgttggc
    15481 tttgccaacg gctccgaagg agcgagcgta gcgagtattg agaccgggaa atgtttcgtt
    15541 cagaaacatt tttcagagag aggggagtgc caatctgcaa aaaactcaca aaatctgacc
    15601 gcttgtctgt tactcaaaat gctccaatcc atattgatac agggcatttt tcttatagcc
    15661 gaaggtttct gccacaatgg cagcggcttt tttaagcgga agttcttggc agagtaatgt
    15721 cagtaatttg atcgcttggc tggaaaaggc ttcctcacta ctttgcggtt taccttcaac
    15781 caccagaaca atttcgcctt taattcggtt gctatcctca tttagccatt caattaagtt
    15841 acccagttga tctccataaa tggtttccca cgttttcgtg atttcacgcg ccattaccac
    15901 atagcgatct tcgccaaaaa cagtctgcat atcggcaaga gtgtctaaaa tacggtgggt
    15961 ggattcataa aaaatcagtg tgcgaggctc gttttcaagc tcggtaagtt tatcgcaacg
    16021 ggattttgtt tttgccggta agaacccttc aaaacaaaaa cggtctgaag ctatgcctga
    16081 agcacataag gcagtaacag cggcacaagc cccggggatc ggcacaactt ttaccccggc
    16141 ttggcggcaa tgacggacaa gatgaaagcc cgggtcgcta atcagtggcg ttccggcatc
    16201 ggaaattaac gcaatgtttt ccccttttgc taatttttcg actaaaacga ccgctttctg
    16261 ctgttcatta tggtcgtgca aggcgaaaaa cggtttttta atgccgtagt ggctcagcaa
    16321 taaaccgctg tggcgagtgt cttcggcggc gactaaatcg acagatgaaa gggtgtctaa
    16381 cgcccgttgg gtaatatcgc ctaaattgcc gataggcgtg gcaacaatat ataaaattcc
    16441 gtaatttggg ttgattccag atgaattttg tctgtttttc atttgctttt agctcgtttg
    16501 ataagtaaga tttcacatta aatttagcac attatattct attgaggatc tgacaatggc
    16561 aactatttta aaccatacaa tgaaaaaggc attggtgcca acggcgattg cattattcat
    16621 ttctgcctgt acatcggtaa atcctgtcac cgaatccatt aaaaatgaag cctattcaag
    16681 ctcggaattc tatattaata aagcagatca aaccaaagaa gcggaagata aaatttcgta
    16741 tcaactttta gctgtacgca aattaattga tgaaaataag gaatatgagg ctcaaaatac
    16801 ctttagtgaa attctgaccg cagagatgaa cgaggtgcaa aagttagagt atgccttggt
    16861 ttccgcccag cttgccgcct tacaagggaa aaacgagcaa gcaacaagcc aattaaaagc
    16921 cattcaagat ccgctattaa gttcggcaca gcgtttgcgt tatattcaaa cccaagcccg
    16981 tattgctgcc aatcagaaag atgtgattgg cattgtgcgt gcaagatcgc aacttaacaa
    17041 tttctataaa atgaaccgtg aacgccaaga aaataacgat attatttggc aaaccttgcg
    17101 tgatgcaaat cgaggaatgc tggaaaaaac tgtgcctgaa gcgggtgaga tggagcttgc
    17161 tggttggttg gcattaatta atatctataa tcaaaatgtg acgacaccgg cacaaatgcc
    17221 acaagcaatt aataattgga aagcccaata cccgagccat agtgcggttg ccgtaatgcc
    17281 aacggaatta caaggggtga gtaatttcca acaaacccag ttaaacagtg tcgcattgct
    17341 attgcctcta agtggtgatg caaaaatttt gggtgatatt atcaagcgtg gttttaatga
    17401 tgctaaggct gatgactcaa ccgcagtgca aacttttgat accgattcat ctgatgttaa
    17461 ttccttaatt tcacaggcaa aacagcaagg ggcaaatgtg attgtcgggc cattgctcaa
    17521 atctcgtgtt gatgaaatgc tacttagccc agaaatccaa aacgtgaata ttttggcact
    17581 gaatgccact gaaaatgtgc gtaatattgc tcaggtgtgt tactacggtt tatctcctga
    17641 atctgaggca caatcagggg cggaaaaaat ttatcaagac ggtaatagtg tcgctatcgt
    17701 tgcggcccct caagatgact acggcaaccg ctcggcagaa gcctttgcca aacgctggcg
    17761 tcagcttacc aatagcgatg ctgatgtgcg ttactacaac caaccgcttg atgccgttgc
    17821 cgctattcaa aatgcaggtg tgagcaaagc agctttatat attttgggga atgccgagca
    17881 agtattagaa attaaacaag gtattgatag ctcaacctta aaagatcgtt tagcgatcta
    17941 tacctcatct cgcagtaatt cgccaaataa cggcattgat ttttacactt caatggaagg
    18001 ggtaaaattt agtgaagtgc cactgttagc tgatcaaagt tctagtgaat ataaaaaagc
    18061 cgagaattta gccaatagcg atttctcaat gatgcgttta tacgcaatgg gggctgatgc
    18121 gtggtcattg gcaaccaaat tcaatgaatt ccgccatatt ccgggctaca aaatttcagg
    18181 tttaaccggt gagttaaaag ccggacaaca ttgtaatatt gaacgtagtt tatcttggat
    18241 gcaatatcgc aatggagcta ttcaacaagc ttattaataa acgggaacaa ggctcgtttt
    18301 atgaacaaaa agcccgagtg tttttagaac agcaagggct tcgttttgtt gccgctaacc
    18361 aagcatttaa aggtggcgaa ctggatccga ttatgcaaga cggacaaacc gttgtgtttg
    18421 tagaagttcg ccagcgtaaa agcaaccgct tcggttcagc cgttgagagt attgattacc
    18481 gcaagcaaca aaaatggcaa aatgccgcta atatgtggtt gcttaaacaa aaccaaagtt
    18541 tagaaactgc aaattgccgt tttgatgtgg ttgcttttga aggaaaccaa acgcctgtgt
    18601 ggattcccaa ctttttaggt tagaaagaat gttaaataaa atccaagata gatttaccga
    18661 aagtattcaa acccaaattt ccgcctctga gctattgccg gctaaattaa gtgaagcggc
    18721 aaaccgcatt gttgcctgtt tgttgcgtgg cgataaagtt atcgtctgcg gacacggtcg
    18781 ctcttacggt aatgccgaat tattggtcag ccacttactt caccgttatg aactggcaag
    18841 accgagtttt gctgccgtac aactgcaatt aagcggtgta ttggcaggag ttgtggcaca
    18901 agataacgag ttagaggctg tttataaaaa acagttgcag gcggttgcaa aagagggtga
    18961 tctcttcatt gttttttcgc ctgtgggtaa tgaagaatgt atattgcaag ctattcatac
    19021 ggcgaaaaat caacatttag atttagatat tattgcttta acaggcagta ataacgacca
    19081 cacactcggc ttattagatg aaagtgatat tgaaatttca gccccttcaa ccaatcaact
    19141 gagggtgatt gaaagccatc atttttatgt gaatttatta tgtgaactta tcgaccattt
    19201 actattttcg taaggagaga ttatgaacaa attagcaaat aaactattaa agggcagcct
    19261 attagtatta ggttttatct ctttacaagg ttgtatcacc acggcggtgg tgacttctgc
    19321 ggcggttgca actaaagtgg cgacagaccc acgcaccacc ggcacgcaag ttgatgatga
    19381 aattttagaa gaaaaagtcg cttacaacat taataaagac gagcaaatca aacaagaagc
    19441 ccgcattaac gtggtggctt acaacggcaa agtgttatta atcgggcaag caccaagtat
    19501 ggacgtagtg gaaaatgcga aaaacctcgc tgctggggcg gagggcgtga cggaaattta
    19561 caacgaaatc cgccaaggcg aaaaaatcgg ctttggacag attacacaag acagctggat
    19621 taccacccaa gtgaaatcca aactgttagt aaatagcgag gtaaaagcca ccgaagtgaa
    19681 agtcgtcacc gaaaacggcg aagtgttttt aatgggcaaa gtcagccaaa accaagctga
    19741 tgccgccgcc gaagccgctc gcaatgtagg tggagtaacc aaagtggtga aagtatttag
    19801 ttatgcgcag taattgataa gctcaattag tttatatttt tgtaactcac aagcggtcta
    19861 aatttgtcat ttttttgcaa atttagaccg cttgtttgat ataagaagat gaccttagat
    19921 aggaagataa aatctatgag tgggtgatcg taatgatgat ttttaattca tttgactcga
    19981 aaaatatgca caccctatca acataaggat tcccttgact cttcattgct ttgagcgtag
    20041 atttaaactt aaactatgtc ttgaacatag tggaatcaag acacaaatca aaggagcaga
    20101 atatgctatt caaagaacaa ggatatgatg agtttcttgc agagcaaatt aaagctggtc
    20161 aagaagcaat agctcgagga gaaaccttta ccaaagaaca aatcaataac gaattaaatc
    20221 atttatttgc ggatatgcag aaacgtcagc aattacgaga aaatgaaaat ttgtttgagg
    20281 ttaattatgc ctaaatcttg ggagctgtca cttaccgcaa ggtctaatgt cattgagatt
    20341 ttacagtcgg tagaagatta tacagggcat tggttgagcg cagtttcact ttgggaacaa
    20401 ttacaaaata aactcgatct cattgcattt atgcctacaa tagggcgacg tagagaagat
    20461 ggcacattag aaacattctg ccgcaattat cgtattgtct atgag
//
LOCUS       DANLRK010000021        20481 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.021, whole genome shotgun sequence.
ACCESSION   DANLRK010000021 DANLRK010000000
VERSION     DANLRK010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 20481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20481
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.021"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(<1..201)
                     /locus_tag="PXA33_000594"
     CDS             complement(<1..201)
                     /locus_tag="PXA33_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5119956.1"
                     /translation="MITSNLERSFHGLNIAQHNYKIFLDESGGNIGKLKHTPNDKKGS
                     NFNRTISSHIGVPPVFVLGGIAQ"
     gene            complement(228..902)
                     /gene="cas4"
                     /locus_tag="PXA33_000595"
     CDS             complement(228..902)
                     /gene="cas4"
                     /locus_tag="PXA33_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HDL5119957.1"
                     /translation="MGDLQNSAEILPLVQPKFGDDKRSISLSALQHYAFCPRQCALIH
                     NEQVWAENYLTAQGQALHERVDSGEPEVRKGIRFERTVHVAAEKLGISGILDLVERDL
                     KTGELKPVEYKRGKPKLDPIDEIQLCAQALCLEEMTEQTINEGALWYMQTRHRLPIIF
                     STELRTKTLATIAEVRSLLDSGTTPPPEYSKRCKACSLIDLCQPKLLERDRSKGYVVG
                     VFGSLL"
     gene            complement(902..1765)
                     /gene="cas7c"
                     /locus_tag="PXA33_000596"
     CDS             complement(902..1765)
                     /gene="cas7c"
                     /locus_tag="PXA33_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas7/Csd2"
                     /protein_id="HDL5119958.1"
                     /translation="MSIQNRYEFVFFFDVTNGNPNGDPDAGNMPRLDPETSKGLVTDV
                     CLKRKIRNFIEMSYENEAGYEIYVKEKSVLNLQNKRAYEALGIESEAKKLPKEEAKAR
                     EITAWMCKNFFDIRTFGAVMTTEVNSGQVRGPVQLAFAQSIDPIIPLEVSITRMAVTN
                     EKDLEKERTMGRKYIVPYALYRVHGFISAKLAEKTGFSDEDVQKLWQALQLMFEHDRS
                     AARGEMAARKLIVFKHDSELGNQPAHKLFDSVKVERINGEKDTPAKDYDDYCISVQTE
                     GLTGVTVEELL"
     gene            complement(1775..3556)
                     /gene="cas8c"
                     /locus_tag="PXA33_000597"
     CDS             complement(1775..3556)
                     /gene="cas8c"
                     /locus_tag="PXA33_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas8c/Csd1"
                     /protein_id="HDL5119959.1"
                     /translation="MITALTRYYYRLAAQEDRLTGEAKVPSYGFSEEKIGWVLELDSK
                     GNLVNVISNLTEDKKPKPKLMSVPRPEKRTSGIKPNFLWDKTAYVLGIESNPDKATAK
                     DQPINFAEKTFEAFKQYHLDLLADSNDEGLMAIRNFLQQWQPTHFAESICPREILDAN
                     VVFKLVGTSGYIHQREAAQTLWTSLLINQSDNAEKGLCLISGKLSPIQRTHPPIKGVF
                     GGQSSGGSIISFNKESFTSFGKEQGSNAPVSEQAAFAYTTALNYLLRTEKHRLTIGDA
                     STVFWAEADNTTQAEAAEGFFAALMTPPDDEQENQKIFEELQKISKGRPLAEISPELS
                     ENTRFYVLGLAPNAARISVRFWLDTTFGQLAKNLSEHWQDLALEPCPWKTPPSIWRLL
                     LQTASLGKSENISPVLAGEMARAVISGHLYPMSLLSQLVTRIRADGDINGLRVALMKA
                     VLQRKFRKGFIKEGVPMSLNKESDNQAYLLGRLFAVLERIQSQALGELNAGIADRYYG
                     SASAVPFSVFPRLLSGAKHHLSRLRKDKGGMAVNLDKDLGEIIASLPENFPRHLSIDE
                     QGRFAIGYYHQKQSYFAKKETTESIEN"
     gene            complement(3578..4255)
                     /gene="cas5c"
                     /locus_tag="PXA33_000598"
     CDS             complement(3578..4255)
                     /gene="cas5c"
                     /locus_tag="PXA33_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas5"
                     /protein_id="HDL5119960.1"
                     /translation="MPNKIRLHIWGDYACFTRPEMKVERVSYDVITPSAARGILAAVH
                     WKPAIRWVIDKIYVLQPIQFESVRRNELGSKISASKISGAMKRKTVADLYTVIEDDRQ
                     QRAATVLKNVGYVIEAHAVLTKMAGEAETVTKHIEMFKRRAKKGQCFQQPCMGVREFA
                     ANFALIDDDEPLPECFLAENERDRDLGWMLHDIDFEHGNQPRFFRAEMKNGVIDVPPF
                     YAEEVKS"
     gene            complement(4497..6282)
                     /locus_tag="PXA33_000599"
                     /pseudo
     CDS             complement(4497..6282)
                     /locus_tag="PXA33_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250669.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
     gene            complement(6272..6733)
                     /locus_tag="PXA33_000600"
     CDS             complement(6272..6733)
                     /locus_tag="PXA33_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5119961.1"
                     /translation="MFKPHGSLDWFMAGDVPMTSCIGKVEEPLIITPGVGKYKKGYGQ
                     PFDAHREKANEAIDSASSILCIGYGFNDDHLQTHLTGKIKSGVKTLLLTRGLSENARN
                     VVKEAPNCKALIFDGDKAGTRVVSKQEETFIPNVHWWDVEFFVKEILENGK"
     gene            complement(6892..8028)
                     /locus_tag="PXA33_000601"
     CDS             complement(6892..8028)
                     /locus_tag="PXA33_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL5119962.1"
                     /translation="MLLRKAFKFEIRPNGEQVRKMKQFCGCSRFVFNKALAWQNEQYE
                     QDNHHKFSYTKLANLLPQWKKELVWLKDCHSQVLQQSLKDLESAFKNFFQKRADLPKF
                     KKKGVKESFRFPQGCKIEQKNDRLYLPKIGWVRYRNSREIVGEVKNVTVSMKCGRFFV
                     SIQTEFKQEIPTHKGGEIGIDMGIARFATLSNGEFFEPLNAFKTHKGKLAKLQKQLKN
                     KVKFSKNWQKLKDKIGKLHHKIANCRKDFLHKISTQISKNHAMIYVEDLQVANMSKSA
                     KGTAEVHGKNVKQKSGLNRVILDQSWFEFRRQLDYKTQWLGGFLLAVPPQNTSRTCPC
                     CNYTAKENRQTQENFECVECGYTENADVVGAMNVLTRGQAIVQA"
     gene            8075..8491
                     /gene="tnpA"
                     /locus_tag="PXA33_000602"
     CDS             8075..8491
                     /gene="tnpA"
                     /locus_tag="PXA33_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005720092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HDL5119963.1"
                     /translation="MKKETEIRRGRHVVFNLHVHLVFVTKYRREVFTQAILNDLHSIF
                     ATICADFEATLVEFDGEDDHVHLLVEYPPKVAISHLVNSLKGVSSRMIRKKNYPSIRK
                     KLWGNQLWSPSYFAGSCGGAPISIIRQYIEQQQTPD"
     gene            complement(8553..9146)
                     /locus_tag="PXA33_000603"
     CDS             complement(8553..9146)
                     /locus_tag="PXA33_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5119964.1"
                     /translation="MREPLLDEDELKKWLQTIFTDNLVIIVGSGLSCAEGLPGMGALA
                     DRLKERMPECLDDIDKVTWNTISDCLDSEGLEGALLKHQANETIEAAIIKITAEYVLS
                     EEQKAINKCIAENQKLKFSYLLPHISASNPKIARVITTNYDRLIEFAAEYENWGIDSM
                     MVGRYWGKHNPDLSRKLQIRDIRVKGKCPKLVYLIVP"
     gene            complement(9277..11565)
                     /locus_tag="PXA33_000604"
     CDS             complement(9277..11565)
                     /locus_tag="PXA33_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HDL5119965.1"
                     /translation="MEEHTIQSFYAHSGKLSDHSDWQTLLSHANNVGLLAAEFAAPFA
                     SQEIARCTGLLHDLGKYSERYQKRLHGGERVNHSTAGAKIAVERWGPLGKMMAFCIAG
                     HHAGLANGSGEGKNRSTLTQRLQEQFGYGHKDLPVLDAIWQQELNLPEKLPLPPLKPS
                     KNEPFFSYAFFIRMLYSCLVDADFLDTEAFYANLEKRPLERGNYPNLTALHQQFNQFI
                     AQFRERIATQTPNTQEAQRKAEVNRLRSEILDYAVSQAKQETGLFSLTVPTGGGKTFT
                     SMAFALEHAKQYGLCRIIYVIPFTSIIEQNAAEFRKAFGELGTEAVLEHHSTFDHDSL
                     PDKSSRDKLKLASENWDTPVVVTTAVQFFESLFADRSSRCRKLHNIVGSVIILDEAQM
                     LPLNLLRPIMAAIDELARNYQCSVVLCTATQPAIHQANGFYKGFENVREIAPNPTALF
                     EKLKRTTVKHIGIQTDVELQSKLADNPQMLIIVNNRRHARALYDSAKQLEGAYHLTTL
                     MCAKHRSQTLTEIRLRLKQNLPCRVIATSLIEAGVDVDFPLVMRAEAGLDSVAQAAGR
                     CNREGKRLSVESNVWVFQPEEQWKAPPELGLLSGCMRSTVQVHADDLLNPKAINHYFQ
                     AVYEAKGKELDKPNILKACHDAGQSLNFPFQKIAEDFRMITSHLIPVIIPFDQEAENL
                     IDRLRYADQVGGILRKLQPYTVQVPEKALAELYNAGRVETINETNFGKQFYCLIGMDL
                     YDEVAGLSWENVDFLESEKLVL"
     gene            11829..13592
                     /locus_tag="PXA33_000605"
     CDS             11829..13592
                     /locus_tag="PXA33_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory protein YcaO"
                     /protein_id="HDL5119966.1"
                     /translation="MSEQTFITGKDAALEDSIATFQHKLKTLGFNIEEASWLNPVPNV
                     WSVHIRDADCPQCFSNGKGASKKAALASALGEYFERLSTNYFWADFYLGQEIANSEFV
                     HYPTEKWFPIDDEAIPPMEMMDEFLWEYFDPNGEITPELLVDLQSGNYDRGIVTLPYV
                     RQSDNETVYIPQSIIGNLFVSNGMSAGNTKNEARVQGLSEVFERFVKNRIITEAISLP
                     EIPQSVIDGYPTIKASIEKLEQEDFPILCYDASLGGEFPVICVILLNPNNGTCFASFG
                     AHPNFQVAFERTVTELLQGRSLKDLDVFAPPSFNNEDVADHANLETHFIDSSGLISWD
                     LFKRDADYPFVHWDFSGTTEQEFNNMMAIFNKHNQPVYIMDYEHLGVYACRILAPGMS
                     NIYPSDDLVYANNNMGMDWREILLDLPHFHHDAETYQELLDELDEQSIDDLTRVREFI
                     GVVAEKGSAMQTLRIGELKSMLHLALGNLEQASDWANWTANMNASVFSAERNNYYRCL
                     IQSIELFLDEERDSEQYRMVFEKMYGKQAVEAAWGAIQGGNPFHGLTASDESLENMTA
                     HQKLLNAYRKVQKAKVNPIAR"
     gene            13689..14651
                     /gene="cmoB"
                     /locus_tag="PXA33_000606"
     CDS             13689..14651
                     /gene="cmoB"
                     /locus_tag="PXA33_000606"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005707797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HDL5119967.1"
                     /translation="MIDFRPFYQQIATTDLSAWLETLPLQLKQWEKTTHADYAKWAKV
                     ADFLPNSTACINLKDKVESISSKPLSEGEVKQITHHLKQLMPWRKGPYHLHGIHIDTE
                     WRSDFKWDRVLPHLAPLKDRTILDVGCGSGYHMWRMVGEGAKMVVGIDPTELFLCQFE
                     AVRKLLGNDRRANLIPLGIEQMQPLAAFDTVFSMGVLYHRKSPLDHLSQLKAQLVRGG
                     ELVLETLVIDGDINDVLVPADRYAKMKNIYFIPSVAALINWLEKVGFKNVRCVDEAIT
                     TLEEQRKTDWLENESLVDFLDPNDHSKTIEGYPAPKRAVILANA"
     gene            complement(14704..17136)
                     /gene="gyrB"
                     /locus_tag="PXA33_000607"
     CDS             complement(14704..17136)
                     /gene="gyrB"
                     /locus_tag="PXA33_000607"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HDL5119968.1"
                     /translation="MSEQTQATSYDSSSIKVLRGLDAVRKRPGMYIGDTDDGTGLHHM
                     VFEVVDNAIDEALAGHCKDIIVTIHSDNSVSVQDDGRGIPVGIHPEEGVSAAEVIMTV
                     LHAGGKFDDNSYKVSGGLHGVGVSVVNALSSKLQLTIRREGHVHEQFYSLGEPDAPLA
                     IIGNTEKTGTSVRFWPSLDIFKNKTEFEYKILSKRLRELSFLNSGVSIKLIDERDGKE
                     EHFKYEGGIKAYVEYLNEGKTHIHNTPFYLTTEKDGIGVEISLQWNDSYNENVYCFTN
                     NIPQRDGGTHLAGFRGALTRALKNYMDNSGVLKKADANIDASGDDAREGLVAVISVKV
                     PDPKFSSQTKDKLVSSEVRGAVESSMNEALSDYLAENPDDAKNIVTKIIDAARAREAA
                     RKAREMTRRKGALDLGGLPGKLADCQEKDPALSELYLVEGDSAGGSAKQGRDRKNQAI
                     LPLKGKILNVEKARFDKMLSSQEVATLITALGTGIGRDDYNLEKLRYHKIIIMTDADV
                     DGAHIRTLLLTFFYRQMPELIENGYIYIAQPPLYKVKKGKQERYIQDNDEMSQYEIDI
                     ALEGAGLYVSEDAPALSGVALENLIAQYNGVQKLIERLSRRYPTLLLNELIYSKPLSV
                     EFAKNQANMTAWSEQFVATLMAKEVGGSFYRINTLFNEERQIYEPEIIVTTHGMDSTY
                     RLDYNFINGNEYARIVALGNELNDLLSDSAYVVRGERRQEISSFAEALDWLVKESRKG
                     LTIQRYKGLGEMNPEQLWETTMDPVARKMLQVNITDAIAADKLFSTLMGDEVEPRRDF
                     IETNALYAQLDI"
     gene            complement(17268..19904)
                     /locus_tag="PXA33_000608"
     CDS             complement(17268..19904)
                     /locus_tag="PXA33_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDL5119969.1"
                     /translation="MELQFETLEYQLQAVNATVNLFVGQPNAATEFSLKAQNDMRFVP
                     NLGLQISDEQLQQNLANLQNRQKIDRTLLVEQGKNFTVEMETGTGKTYVYLRTIFELN
                     RQYGWQKFVIVVPSVAIREGVLHTLETTKSHFNTVFDNPSVNQKFEYKSNQTSRLKSF
                     ASANHIEILVMNIDAFTKESNVINTVNESGDAPIFYIQQANPIVIIDEPQNMETEIRR
                     NAIESLSPLFTLRYSATHKNAYNPIFSLNPVQAYEMGLVKQIEVDSVLAENEVNGAYL
                     SLKEIKTGAKSWSAKIEILFNDKKAMKKKVISVKPNQDLYELSNQNEMYRSGFILSGM
                     DTDSQMVSFSNGTQIFQGAEHSQLKEDVQKMQIRRTIGEHLQKEKKLRPLGIKVLSLF
                     FIDKVDNYRPSAQSPTGGKFAKWFEEIYLELAKEEPQGVHNGYFSQDKKGVAKDTNGT
                     TQADNETYQLIMRDKEKLLSLENPLRFIFSHSALKEGWDNPNVFQICTLNDTASEMKK
                     RQEIGRGLRLPVNQQGLRIYDRHLNTLTIIANESYEQFAATLQREIEEDCGVQFAKSN
                     VKKREDKKAVRYRKDFSLHPEFAAIWERIKHQTTYRVDFSNAELIESAVEKIRKMPDI
                     EATKIKHSKVKLEMNETGISTNYRSSNETAVNTQWAIPDLLSEIQKKTGLTRATLCKI
                     LQKSDRLQEVFRNPQRFIDLIAEKINLALQQIMTDGVEYHKIAGKTYEMTLFGDFDFY
                     LNQYTFEVQDKTKTIYEDYIALDSQTENIFARHCESSEQVEFYFKLPKRFKIPTPLGS
                     YNPDWAVVFKGENKIYLVAETKNTGKGIQAGVDEEKLDESERMKIDYARKHFKGLQRD
                     YKDLDYRVVQKVEELSAEYCKI"
     gene            complement(19914..>20481)
                     /locus_tag="PXA33_000609"
     CDS             complement(19914..>20481)
                     /locus_tag="PXA33_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HDL5119970.1"
                     /translation="IAVQYPEEIDIKSKNGKVAKQFCQRKNLPLYITEISKERIRRAG
                     KQILQNNPENRPLDVGFKVFKLTESHFKQWQSPTSENLADQLEMFIDPVSEKADTQAV
                     LYELLLRLGLKLTAKVRLENKVFWVEENGLIFALLLNAADEEIIQTVIAQQPKKVIAL
                     DRLFNGNDARKKNTELQMQDAGITFFVI"
BASE COUNT         5656 a         4499 c         3916 g         6410 t
ORIGIN      
        1 ttgagcaatc cctcctaata caaatacagg aggaacccca atgtgggaag aaatggttct
       61 attaaaatta ctaccttttt tatcattagg cgtatgtttt aacttcccaa tatttccacc
      121 actctcatca agaaatattt tgtagttatg ctgtgctata tttaatccgt gaaatgatct
      181 ttctaagttt gaagttatca caacaagtcc ctccccaaag taggtaatta taataaagat
      241 ccaaacactc ccaccacata ccccttcgat ctatcccttt ccaacaactt tggttggcag
      301 aggtcgataa gcgagcaggc tttgcagcgt ttgctgtatt ctggaggagg tgtggttccg
      361 ctatctaaga gcgaccgcac ttcggcaatg gtggcgaggg ttttggtgcg tagttcggtg
      421 gaaaaaatga tcggcaaacg gtggcgagtt tgcatatacc acaacgcccc ttcgttaatc
      481 gtctgctctg tcatttcttc caaacataag gcttgggcac agagttggat ttcatcaatc
      541 ggatcaagtt tgggcttgcc acgtttgtat tccactggtt ttagctcgcc tgtttttagg
      601 tctcgctcaa ccaaatccaa aatgccgctt atgcctaatt tttcggctgc aacgtgaacc
      661 gttcgctcaa agcgaatccc tttgcgaact tctggctcac cactatctac ccgctcgtgc
      721 aacgcttgac cttgtgcggt gagatagttt tctgcccaca cctgttcgtt gtggatcagc
      781 gcacattgac gtgggcagaa ggcatagtgt tgcaaagcgg aaagcgaaat tgaccgcttg
      841 tcatcgccaa attttggctg aacaagcggt aaaatttccg cagaattttg caaatcgccc
      901 attacagtaa ttcttcaacg gttacacccg ttaagccttc ggtttgtacg ctgatacagt
      961 aatcgtcata gtctttcgct ggcgtgtctt tttcaccgtt gattcgctca acttttaccg
     1021 agtcaaacag tttgtgtgca ggttggttgc ctaattcgct gtcgtgttta aatacgatca
     1081 gtttacgagc tgccatttcg cctcgtgcgg cagaacggtc gtgttcaaac ataagttgca
     1141 aggcttgcca aagtttttgt acatcttcat cagaaaagcc cgttttttca gcgagtttag
     1201 ctgaaataaa gccgtgaaca cgataaagtg cgtaaggcac gatatattta cgccccatag
     1261 tccgttcttt ttcaagatct ttttcgttgg tcactgccat tcgtgtgatc gatacttcta
     1321 gcggaataat cggatcgata gattgagcga acgccaattg cactgggcct cgcacctgtc
     1381 cgctgtttac ttcggtagtc attaccgccc caaaggtgcg aatatcgaaa aagtttttgc
     1441 acatccaagc ggtaatttct cgggctttag cttcctcttt tggtagtttt ttcgcttccg
     1501 attcaatccc taatgcttca taagcacgtt tgttttgtaa attcagaaca cttttttctt
     1561 taacataaat ttcgtaaccc gcctcgtttt catagctcat ttcaataaaa ttgcggattt
     1621 tgcgttttag gcaaacatcg gtgactaaac ctttactggt ttcaggatca aggcgtggca
     1681 tatttcctgc atctggatca ccatttgggt taccgttggt tacatcaaag aaaaaaacaa
     1741 attcatagcg gttttgaata gacatagtgt ttccttagtt ttcaatagac tcagtggttt
     1801 cttttttagc gaaatagctt tgtttttggt ggtaataacc aatcgcaaaa cgcccttgtt
     1861 catcgatact taaatggcga gggaaatttt ccggcaagct ggcaataatc tcacctaaat
     1921 ccttatccag attaaccgcc attccgccct tatcttttct caagcgagat aaatggtgtt
     1981 ttgcaccgct taataagcga gggaataccg aaaacggcac ggcagaagcc gaaccataat
     2041 agcggtcggc aatgccggca tttaattcgc ctaatgcttg gctttggatt ctttctaata
     2101 ctgcaaataa cctgccgagc aagtaagctt ggttatcgct ttctttattt aagctcatag
     2161 gaactccttc tttaataaaa ccttttctaa attttcgttg taagactgct ttcatcaacg
     2221 ctactcgcaa accgttaata tcgccgtctg ctcggattcg agtcactaac tgtgaaagta
     2281 aactcatcgg atataaatga ccgctaataa ctgcgcgagc catttcaccc gccaagactg
     2341 gagaaatatt ctcactttta cctaatgaag cggtttgtaa taatagtcgc caaatggaag
     2401 gtggtgtttt ccatgggcaa ggctcaagag cgagatcttg ccaatgctcg ctgaggtttt
     2461 ttgccagttg cccaaaggtg gtatccagcc aaaaacgtac cgaaatgcga gctgcatttg
     2521 gagcaagccc caacacataa aaacgggtgt tttcagataa ttctggggaa atttctgcaa
     2581 gcggtcgtcc tttgctgatt ttttgcaatt cttcaaagat tttttggttt tcttgctcat
     2641 catctggagg cgtcattaaa gcagcaaaaa agccttcagc agcttcagct tgggtggtat
     2701 tgtctgcttc tgcccaaaag acagtgctgg catcaccaat agttaaacga tgtttctcgg
     2761 tgcgtaacaa ataatttaaa gcagtagtgt aggcaaaggc ggcttgttca gaaacagggg
     2821 cattactacc ttgttccttg ccaaatgaag tgaaggattc tttattgaaa gagatgattg
     2881 aaccaccgga actttgccca ccaaataccc ctttaatagg aggatgagtt cgttgaattg
     2941 gcgataattt gcctgaaatt aagcataaac ctttttcagc attatcactt tgatttatca
     3001 acaaacttgt ccaaagggtt tgtgcagctt ctcgctgatg aatataaccg cttgtgccga
     3061 ctaatttaaa cacgacattc gcatcaagaa tttctcttgg gcagattgat tcggcaaaat
     3121 gtgtgggttg ccattgttgc aggaaatttc ttatcgccat taaaccttca tcattggaat
     3181 ctgcaagcaa atccaaatgg tactgtttaa atgcctcaaa ggttttctcg gcgaaattga
     3241 tgggttgatc tttggcggtg gctttatcag gattactttc aatgcctagc acataagcgg
     3301 ttttatccca cagaaaattg ggcttaatgc cagacgtgcg tttttcaggg cgaggtacgc
     3361 tcatcaattt cggttttggt ttcttatctt ctgttaaatt tgagatcaca ttgaccaaat
     3421 ttccctttga atctaattct agtacccaac cgattttttc ttcactgaaa ccataagaag
     3481 gcactttagc ttcgcctgtt aagcgatctt cttgtgctgc aaggcggtaa taatagcgag
     3541 ttaaggcggt aataatagcg agttaaggcg gagagaatca tgatttgacc tcctctgcat
     3601 aaaatggtgg cacatcaatc acgccatttt tcatttccgc acggaagaaa cgaggttgat
     3661 taccgtgctc aaaatcaatg tcgtgcaaca tccagcctaa atcccgatct cgctcatttt
     3721 ctgccaaaaa acactcaggt aacggttcat cgtcatcaat caaggcaaaa tttgcggcaa
     3781 attcacgcac gcccatacaa ggctgctgga aacattgccc ctttttcgca cggcgtttaa
     3841 acatttcaat atgcttggtg acggtttccg cctcgcctgc cattttggtc agcactgcgt
     3901 gagcttcgat cacataaccg acatttttca gcacagtagc agcacgttgt tggcggtcgt
     3961 cttcaatcac agtatataaa tccgccactg ttttacgctt cattgcacca ctgattttac
     4021 tggcagaaat tttactgcct aactcattac ggcgaacaga ctcaaattga atcggttgta
     4081 atacataaat tttatcaatc acccagcgaa ttgccggctt ccaatgcacc gcagccaaaa
     4141 tcccccgtgc cgcagacggc gtaatcacat cataagacac ccgttctacc ttcatttcag
     4201 gacgggtaaa acaagcataa tcaccccaaa tatggagcct gattttgttt ggcataatag
     4261 aatgctcctt ttaattatgt aacacacacc tccccgaagg gaggtgaaat ttctttagct
     4321 tatattataa ctgctttggt ttcaacccac gcactcagta aaatgcgaaa aattttatgg
     4381 cttattatat taagcggtcg gatttactta gaaagttgca aagttatttg agtatttaac
     4441 cgcttgtatt tcaaaaatag tcagaaaaat aacagcattc taggtacaat atttttctaa
     4501 gaacgaactt gtctccgcaa tgcctctact gcgtttgaca cagcatttgg tttgggctcc
     4561 tggtttgccc aatcagacca gaattggcgt gtagcacttt ctggggctat atgcggttta
     4621 gcaagtttga ttcgtgtagg taatagaatc gaatcgccta gcaataacgc ttcgcctaaa
     4681 tcaagtaacg gcaatatatc agtaattcca accatagatt ccggcaataa acgctttaca
     4741 acactttgat cttgaccatt tgttaatcgt aaaaccaaga aattattaca ttgactcaaa
     4801 attgttcggc ttacatctga aggacgttgg ctaacgacca acaaagacac gccatatttt
     4861 ctaccttctt tcgcaatccg ctcaaaggct tccaatgcgc gtttttccgc tgcttccgct
     4921 gaatctcgaa cagggagata taaatgtgct tcatcgcaaa taatcgttag tggatggcgg
     4981 gaatctttat ccatccaaaa ttgaatgttg tataaaaggc gggcaagaac tcccgtaaca
     5041 atcggtaaaa catccgacgg gacttccgaa aaatcaataa tttttatccc tttttcgctc
     5101 tcagaagcag aaaacagttt tgacaacata ttttccagcc acccatagct gttgcactct
     5161 actggcggtt ggaacataaa accgcaacgt ctgtcatcta ttttggcttt catccttccc
     5221 ataaacctag acaacttact ataccaagga ccttgctttg ttcctgaacc tccctcttta
     5281 ggtaccatct cagaattatc tttatctaaa cgagctaaca ataattcaat agaatacgga
     5341 attggcgaat caacagtaaa cgtttccaaa acatcatttc tttcttcctt attcaatgtt
     5401 tgctctttta atgcgcgaac atgctcagta aaacgcgccg cctgattcgg agcattatca
     5461 tcactgcgat ctaaaattaa agccagcatt tattcccgat tgagcagcca atagggaata
     5521 aataaactgc gttcatctga accatccaaa tctcccggtc cagcaatacg gtattgctga
     5581 gcgatagcgt ttttaccttc gcatagagtg ctatattcac cgtgcatatc gaatacaata
     5641 agattagcag cttttaaagc tgctgcacgt tccaatatta atgcaactga ccagctttta
     5701 ccggaaccag tactacccaa aatagcagcg tgccgttgaa agaatttatc tccgtttgct
     5761 atagctacag ccgtttcatc agccataaaa tgacctaaag tcagccgttt catcagccat
     5821 aaaatgacct aaagtcagcc gttcattttc ttttacgtta gcggacaaaa gattcataaa
     5881 taattgcaga ttgctgccac taatatgaaa gcaatctcta tcaatttgcg gaaaacaatc
     5941 cgctccccgc ttaaaagtgt tgctcaaact accatcaaca gctttataag taccgattaa
     6001 tactgccttg ataaagtcat cctgactttc acccagagga atttcaccct cattagatat
     6061 ttcttctagc agcaattttt cactgataga acgcgttacc cgttcgataa tgccgataag
     6121 gtactcacta gccgttctgc cttgaatcgc aatcagatta cccacagcaa tactagtcat
     6181 ttgttgatgc tcttcaacag aaatcaatac acggctggta tctactgtta caaccctgcc
     6241 caaacgggca ttgtctttaa aattcaatgt ttcatttgcc attctctaaa atctctttta
     6301 caaaaaactc aacgtcccac caatgaacat taggaataaa agtttcttct tgcttgctaa
     6361 ccacccttgt tccagcctta tctccatcaa aaatcagagc cttacaattc ggagcttcct
     6421 tcaccacgtt tctggcattt tctgaaagtc ctcttgtcag aagtagtgtt ttcacaccac
     6481 ttttaatttt gcctgtcaaa tgtgtttgaa ggtggtcatc attaaaacca tagccgatac
     6541 ataaaattga agaagcagaa tcaatagctt catttgcttt ttctcgatga gcatcaaatg
     6601 gttgtccata gcctttttta tacttcccaa caccaggcgt aataattaaa ggctcttcca
     6661 ctttacctat acaacttgtc attggaacgt ctccagccat aaaccaatct aaactaccat
     6721 gcggtttaaa gattttgata tgatctcgtt gacatcctcc cctgcctaaa ggcaggggat
     6781 tcctactagc tcccacagca agctgtgggc tactctcggt gggtttctac tgctgatctc
     6841 tattgagatt cacttcatag acgcttcggg tatgtcctac cctgatattt attatgcttg
     6901 gactattgct tgcccacgtg taagaacatt cattgctccc actacatcgg cattttccgt
     6961 atagccacat tctacgcatt caaaattttc ctgtgtttgg cgattttctt ttgctgtata
     7021 gttgcaacaa gggcaagtac gactggtatt ttgtggagga actgctagca aaaatccgcc
     7081 taaccattgc gttttgtagt ccagttgtcg tctaaattca aaccaagact ggtctaatat
     7141 cactcggttt aagcctgatt tctgtttcac atttttgccg tgtacttccg ctgtgccttt
     7201 ggctgatttg gacatattcg ccacttgcaa atcttcaaca tagatcatcg cgtggttttt
     7261 gctgatttgc gttgagattt tatgcaggaa atcttttcgg cagttggcga ttttgtgatg
     7321 taacttgccg attttatctt tcagtttttg ccagttttta ctgaacttca ctttattttt
     7381 tagctgtttt tgcagttttg ccaattttcc tttgtgggtt ttaaacgcat ttaacggctc
     7441 aaaaaattct ccattggaaa gggttgcaaa acgagcaatg cccatatcaa tgccgatttc
     7501 tccgccttta tgcgtgggaa tttcctgttt aaattccgtt tgaatgctca caaaaaagcg
     7561 tccgcatttc attgaaacgg tgacattttt tacttcccca acaatttcac gactgttgcg
     7621 ataacgcacc caaccgattt tcggcagata caagcggtcg tttttctgct cgattttgca
     7681 accttgcgga aaacggaaac tctcttttac gccttttttc ttgaatttgg gcaaatccgc
     7741 acgcttttga aagaagtttt taaacgcact ttcaaggtct tttaacgact gttgaagcac
     7801 ttgtgaatgg caatctttaa gccataccaa ttcttttttc cattgcggaa gcaagttggc
     7861 gagcttggta taactgaatt tgtgatggtt atcttgttcg tactgctcat tttgccacgc
     7921 caatgccttg ttaaacacaa agcgagaaca gccgcaaaac tgcttcattt tgcgaacttg
     7981 ttccccattt ggtctgattt caaatttaaa ggctttacgt agtaacattg cacgaattag
     8041 aatacaaaaa ttgtgcttat tctacacttg gcctatgaaa aaagaaacag agattagacg
     8101 tggtcgccac gttgttttta acttacacgt tcatttagtt ttcgtgacta aatatcgcag
     8161 ggaagttttt actcaagcaa ttttgaacga cttacactca atttttgcaa cgatttgtgc
     8221 tgattttgaa gccacattag tggaatttga tggtgaagat gatcacgttc atctacttgt
     8281 ggaatatccg cctaaagtcg ctatttctca tcttgtaaac agtttaaaag gcgtttcgag
     8341 ccgaatgatt agaaagaaaa actatccgag cattcgcaaa aaattatggg gaaatcaact
     8401 ttggtcaccg tcttactttg ctggtagttg tggtggtgca ccaatatcga ttattcgcca
     8461 atatatcgag caacaacaaa cacctgatta gttagaaatt tgaaacggct ttgccgttta
     8521 taccttatat ccacgcccta aaggacgtgg ttttacggca cgattagata aaccaatttt
     8581 ggacatttac cttttactct aatgtctctt atctgcaatt tcctactcaa atcaggatta
     8641 tgttttcccc aataacgacc gaccatcatt gaatctattc cccaattttc atactctgcc
     8701 gcaaactcaa tcaatctatc ataattagta gtaattacac gggcaatttt aggatttgaa
     8761 gctgaaatat ggggaagcaa ataagaaaat ttgagcttct gattctcagc aatacatttg
     8821 tttatcgctt tttgctcttc ggagagtaca tactcggctg ttattttaat aatagcggct
     8881 tctatcgtct catttgcttg atgcttcaac aatgcacctt ccaagccttc agaatccaga
     8941 caatcagaaa tggtattcca tgttacctta tctatatcat caagacattc aggcattcgt
     9001 tcttttaatc tatcagctaa tgctcccatt cccggcaaac cttcggcaca ggacaaaccc
     9061 gaaccgacaa taatgaccaa attgtcagta aaaatagttt gcaaccattt ttttaattca
     9121 tcttcatcta ataatggttc tctcatttta cgtttccctt ccaaatatct attcttacaa
     9181 actagctgtt tactacgaga cgtcataggc tatttggtat attaaagtat atctatgcaa
     9241 aattaaactt ataaagaagt taaaataaac attcatctat aaaaccaatt tctccgactc
     9301 aagaaaatcc acattctccc aactcaaccc cgccacttca tcatacaaat ccattccaat
     9361 taagcagtaa aactgtttgc cgaagttggt ttcattaatg gtttcaactc tgccagcgtt
     9421 atagagttcg gctaatgctt tttccggtac ttgcacagtg taaggctgga gttttcttaa
     9481 aataccgcct acttgatccg catagcgtaa acggtcaata aggttttcgg cttcttggtc
     9541 gaaagggata atcaccggaa ttaaatggct tgtgatcatt cggaaatctt cagctatttt
     9601 ttggaatggg aaattaaggc tttgcccagc gtcgtgacac gcctttaaaa tatttggctt
     9661 atccagctct tttcctttag cctcataaac cgcttggaaa taatgattga ttgctttcgg
     9721 attaagtaga tcatctgcgt gaacctgaac cgttgaacgc atacagccag aaagtaagcc
     9781 tagctctggt ggtgctttcc actgttcttc aggttggaaa acccaaacat tgctttctac
     9841 agataaacgt ttcccctctc ggttgcaacg ccctgctgct tgagccactg aatctagtcc
     9901 tgcttcagct cgcataacca gtgggaaatc gacatcaaca ccggcttcaa ttaaagaagt
     9961 ggcaatcact cggcaaggta ggttttgttt taggcgaaga cgaatttcag tcagcgtttg
    10021 tgagcggtgt ttagcacaca ttagcgtggt gaggtgataa gctccctcta actgtttcgc
    10081 tgaatcatat aacgctcggg cgtgacggcg gttattcacg attatcagca tttgtgggtt
    10141 atctgcgagt ttgctttgca actccacatc tgtttgaata ccgatatgct ttaccgtagt
    10201 acgtttcaat ttttcaaaaa gtgcggttgg attaggggca atttctcgca cattttcaaa
    10261 tcctttataa aaaccgttcg cttgatgaat agcgggttgt gtcgctgtgc aaagcactac
    10321 cgaacattga taattgcgag ctaattcatc aatcgcagcc ataattgggc gtaataaatt
    10381 caacggtagc atttgggctt cgtccaaaat aatcacgcta ccgacgatat tatgcaattt
    10441 gcgacagcgt gaagaacggt cggcaaacaa actttcaaag aattgcactg cagttgtaac
    10501 cacaacagga gtatcccaat tttctgatgc caatttcagc ttatctcggc ttgatttatc
    10561 aggtaggcta tcgtgatcaa aagtgctgtg atgttccaat accgcctctg ttcctaattc
    10621 gccaaaagct ttacgaaatt ccgcagcatt ttgttcaata atgctggtaa acggaataac
    10681 ataaataatg cggcataaac catactgttt agcatgctca agggcaaacg ccattgaagt
    10741 aaaggtttta ccaccgcccg ttggcacagt cagagaaaac agacctgttt cctgttttgc
    10801 ttgagatacg gcataatcca aaatttcact ccgcaagcgg tttacttccg ctttacgttg
    10861 tgcttcctgc gtattaggcg tttgagtggc aattcgctcc ctaaactgtg caataaattg
    10921 attaaattgc tgatggagtg cggttaaatt ggggtaattt cctcgttcaa gcggtctttt
    10981 ttcgagattg gcataaaaag cttctgtatc aagaaagtcg gcatcgacaa ggcaagaata
    11041 gagcattcgg ataaaaaagg cgtaagaaaa gaaaggttcg tttttagaag gctttaacgg
    11101 cggtaagggc aatttttcag gcaaattaag ttcttgctgc caaatagcgt caagtaccgg
    11161 tagatcttta tgaccatagc caaattgctc ttgcaaacgt tgtgtaagtg tagaacgatt
    11221 tttcccttct ccagaaccat ttgctaaccc agcgtggtgg cctgcgatac aaaacgccat
    11281 cattttgccc aaaggccccc aacgttccac tgcaattttt gcaccagcag tagagtgatt
    11341 tacccgttct ccgccatgta aacgcttttg gtaacgttca ctatatttgc ctaaatcgtg
    11401 caatagcccc gtgcagcgag caatttcttg tgaagcaaac ggcgcagcaa attcggcggc
    11461 aagcaagcca acattattgg cgtggctgag taaagtttgc caatctgaat gatctgaaag
    11521 cttccccgaa tgggcataaa aagactgaat agtatgttct tccattttta taccttcaat
    11581 atttccttcc taacacaagc aatactacct ctaacctttt attctgtata tatcctagat
    11641 aatattttct taatctaaat caaacacaaa tttcacttgc aaaattcaag taaaatttga
    11701 ccgcttgtaa tcattttttc taagttttga aatctgttct ttcagcagaa atttcacaaa
    11761 aaatttactt atctatgggg atttttgcta gaatgttgcc atttttacga caagttaaag
    11821 gtattatcat gtcagaacaa acttttatta ccggtaaaga tgctgcactt gaagacagca
    11881 ttgcgacctt tcagcataaa ttaaaaacct taggttttaa tattgaagaa gcctcttggt
    11941 taaatcctgt tccgaatgtg tggtcggtgc atattcgtga tgcagactgt ccgcaatgtt
    12001 tttctaacgg taagggagca agcaaaaaag ccgctcttgc ctctgcatta ggtgagtatt
    12061 ttgagcgttt atctaccaac tatttctggg cggattttta tttaggtcaa gagattgcta
    12121 atagtgaatt tgtgcattat ccaaccgaaa aatggtttcc gattgatgat gaagccattc
    12181 caccaatgga gatgatggac gagtttttat gggaatattt tgatccgaat ggtgaaatca
    12241 cgccggaatt attggttgat ttacaatcag gaaattatga tcgtggaatt gttaccctac
    12301 cttacgttcg ccaatctgac aatgaaacgg tttacattcc gcaaagcatt atcggcaacc
    12361 tgtttgtttc aaacggtatg tcggcaggca acacaaaaaa tgaagctcga gtgcagggct
    12421 tatccgaagt gttcgagcgt tttgtcaaaa accgtattat tactgaggca atcagcttgc
    12481 cggaaattcc gcaaagtgtg attgacggtt atccgacaat caaagcctct atcgagaaac
    12541 tagagcaaga agacttcccg attttatgct atgacgcttc attaggcgga gaatttccag
    12601 ttatttgtgt gatcttgtta aacccaaata acggtacttg ttttgcctct tttggtgcac
    12661 acccgaattt ccaagtggcg tttgaacgta ccgtaaccga gttattacaa ggccgtagct
    12721 taaaggattt agatgtgttt gccccacctt catttaataa tgaagatgta gcagatcatg
    12781 cgaaccttga gacacacttt attgattcaa gcggtctaat ttcgtgggat cttttcaaac
    12841 gtgatgcgga ttatccgttc gtgcattggg acttctccgg cacaacggag caagaattta
    12901 ataatatgat ggcgattttc aacaagcata atcagcctgt gtatattatg gattatgagc
    12961 atttaggtgt gtacgcttgc cgtattcttg caccgggtat gtcgaatatc tatccaagcg
    13021 atgatttggt ctatgccaat aacaatatgg gtatggattg gcgtgaaatc ttgttagatt
    13081 tgccacattt ccatcacgat gctgaaactt accaagaact gcttgatgaa ttagatgagc
    13141 aaagcattga tgatttaacc cgtgtgcgtg aatttattgg cgtggtggca gagaaaggct
    13201 ctgcaatgca aacattgcgt atcggtgagc ttaaatcgat gctgcattta gctttaggta
    13261 acttagagca agcctcagac tgggcaaact ggacagcgaa tatgaatgca agcgtattct
    13321 cagcagaacg taacaattat taccgctgct taatccaatc gattgagttg ttcttagatg
    13381 aagagcgtga ttcggaacaa tatcgtatgg tgtttgagaa aatgtacggt aaacaagcgg
    13441 ttgaagctgc ttggggtgca atccaaggcg gtaacccgtt ccacggttta actgcatcgg
    13501 acgaatcgct tgaaaatatg actgcacatc agaaattact gaacgcttat cgcaaagtgc
    13561 aaaaagcgaa agtaaatccg attgcgagat aattttatat ttttcgtagg ggtaggttct
    13621 gtgcctgtcc tttctattta ctacctttcg ggagggcata ggtctctccc ctagacataa
    13681 atttcaaaat gattgacttt cgtccttttt atcaacaaat tgccacaacc gacctttctg
    13741 cttggttaga aaccttaccc ttacaactca aacaatggga aaagaccacc cacgctgact
    13801 atgccaagtg ggcaaaagtt gcagattttt taccaaattc gaccgcttgt atcaatctaa
    13861 aagacaaggt tgagtctatt tcaagcaagc ctttatctga gggggaagtc aagcaaatta
    13921 cccaccattt aaagcaacta atgccgtggc gtaaaggccc ttatcatcta cacgggattc
    13981 atattgatac cgaatggcgt tccgatttta aatgggatcg tgtgcttccg catcttgcac
    14041 cgttaaaaga ccgcaccatt ttagatgtcg gctgcggtag cggttatcat atgtggcgaa
    14101 tggtaggcga aggggcaaaa atggtagttg gtattgaccc gaccgagctg ttcctgtgcc
    14161 aatttgaggc agtgcggaaa ctcttaggga atgatagacg ggctaatctg attccacttg
    14221 gtattgagca aatgcaaccg cttgctgctt ttgataccgt gttttcaatg ggggtgcttt
    14281 atcatcgcaa atcaccgctt gatcatcttt ctcaactcaa agcccaatta gtcagaggcg
    14341 gtgaattggt gttagaaacg ctggtgattg atggcgatat taatgatgtt ttagtgccgg
    14401 ctgatcgcta tgcgaaaatg aaaaatatct attttattcc ttctgttgct gcactcatta
    14461 attggctgga aaaagtcggc tttaaaaatg tgcgttgtgt tgatgaggca atcactacac
    14521 tagaagagca gcgaaaaacc gattggttgg aaaatgaaag tttagtggat tttttagatc
    14581 caaacgatca tagcaaaacc attgaaggct accctgctcc gaagcgggcg gtgattttag
    14641 cgaatgctta aaatgctagt ttaagagaat aaaaaatccg catgtcaaat gcggattttt
    14701 taactaaata tctaactgag catacaaagc gttagtttca atgaaatctc ggcgtggttc
    14761 aacttcatcg cccatcagcg tactgaagag tttatctgcc gcaatcgcat cggtaatatt
    14821 gacctgtagc attttacgag ccaccggatc catcgtggtt tcccataatt gttccggatt
    14881 catctcacct aaccctttat aacgttgaat agttaagcct ttacgagatt ctttcaccaa
    14941 ccaatctaag gcttcagcaa aactgctgat ttcttggcga cgttcaccac gcaccacata
    15001 ggctgaatca gatagtaaat cgttcagctc gttgcctaat gccacaatgc gagcgtattc
    15061 attaccgttg ataaagttgt aatctaaacg ataggtactg tccataccgt gtgtagtcac
    15121 aataatttcc ggctcataaa tttggcgttc ttcattgaac aaggtattaa tacggtagaa
    15181 actgccgccc acctcttttg ccatcaatgt agcgacaaat tgctcgctcc aagcggtcat
    15241 atttgcttga ttttttgcaa attccaccga aagcggtttt gaataaatta attcgttaag
    15301 taataatgtt gggtaacgac ggcttaaacg ttcaattaat ttttgtacgc cgttatattg
    15361 ggcaattaag ttctctaacg ctacgccact taacgctgga gcatcttcac tcacatataa
    15421 gcctgcacct tctaatgcaa tatcaatttc atattgcgac atttcatcgt tatcttgaat
    15481 ataacgctct tgcttgcctt ttttcacttt ataaagcggt ggctgtgcaa tataaatata
    15541 gccgttttca atcagctccg gcatttgacg gtagaagaaa gttaataaaa gcgtacgaat
    15601 atgtgcacca tccacgtccg catcggtcat aataatgatt ttgtggtaac gtaatttttc
    15661 caaattataa tcatcacgcc cgatgcctgt gcctaaagcg gtaatcaagg ttgctacttc
    15721 ttgtgaagaa agcattttat caaagcgggc tttttccacg ttcagaattt tcccttttaa
    15781 cggcaaaatc gcttggtttt tacggtcacg cccttgtttt gccgagccac cggcagagtc
    15841 accctccacc aagtaaagct ccgataacgc cggatctttt tcctgacaat cagctaattt
    15901 acccggtaag ccgcctaaat ctaacgcccc tttacggcgg gtcatttcac gggctttacg
    15961 agctgcttct cttgcacggg ctgcatcaat aattttggta acgatatttt ttgcatcgtc
    16021 cggattttcg gctaaatagt cggataaagc ctcattcatt gagctttcca ctgctccacg
    16081 cacttctgac gacaccaatt tatctttggt ttgtgaggag aatttcggat ccggcacttt
    16141 caccgaaatc accgctacta aaccttcacg ggcatcatca cctgaggcat caatgttcgc
    16201 atcggctttt ttcaataccc cgctgttatc catatagttt ttcaacgcac gagttaatgc
    16261 cccacggaaa ccggctaagt gcgtaccgcc atcacgctgt ggaatattat tggtaaagca
    16321 gtacacattt tcgttgtaac tgtcattcca ctgtagggaa atttccacgc caatgccgtc
    16381 tttctcagtg gtcaaataga atggcgtatt gtgaatatga gttttgcctt cgtttaaata
    16441 ctcaacatag gctttaatac cgccctcata tttaaaatgc tcttctttgc cgtctcgttc
    16501 atcaattaat ttaatcgaaa cgcctgaatt caaaaatgat agttcacgca agcgtttaga
    16561 cagaatttta tattcaaatt cggttttatt tttgaaaata tccaaacttg gccagaaacg
    16621 aaccgacgtc cccgtttttt cggtattgcc gataatcgct aaaggtgcat caggctcacc
    16681 taagctatag aattgctcgt gaacgtgtcc ttcacggcga atagtgagtt gtaatttaga
    16741 ggaaagtgca ttcaccaccg atacgcccac accgtgcaaa ccgccggaaa ccttatagga
    16801 gttatcgtca aatttaccgc ctgcgtgaag aacagtcata atgacttctg ctgccgaaac
    16861 gccttcttcc gggtgaatcc ccaccggaat tccacgaccg tcatcttgca cagaaaccga
    16921 attatcggaa tgaattgtga caataatgtc tttacaatgc ccggctaatg cttcgtcaat
    16981 cgcattatca accacctcaa acaccatatg gtgtaagccc gtaccgtcat cggtatcgcc
    17041 gatatacatt cccgggcgtt tacgcaccgc atctaagccg cgtaacacct taatactgga
    17101 agaatcataa cttgttgctt gagtttgttc agacattctt attcctacta atttgaaatc
    17161 gaaaaaattg tgccgattat agcagaaaaa tgcgtaaaag tatcgcaaaa caagcggtta
    17221 aattttagtt gaaattgcag tctatctcaa ttctacaaga ccacagatta aattttacaa
    17281 tactccgcag acagctcctc cactttctgt acaaccctat aatccaaatc cttataatct
    17341 ctctgtaagc ctttgaaatg cttacgggca tagtcgattt tcattcgttc actttcatct
    17401 aatttttctt catctactcc tgcttgaata cctttgcctg tatttttcgt ttcggcaacc
    17461 aagtaaattt tattttctcc cttaaacaca actgcccaat cgggattata gctgcctaat
    17521 ggcgttggaa ttttaaaacg tttgggtaat ttaaaataaa actccacttg ctcactagat
    17581 tcacaatgtc gggcgaagat attttctgtt tgggaatcca gtgcaatata gtcttcataa
    17641 atcgtttttg ttttatcttg aacttcaaaa gtatattgat tgaggtaaaa atcaaaatcg
    17701 ccgaataacg tcatttcata ggtttttccg gcaattttgt gatattccac cccgtctgtc
    17761 ataatttgtt gtaaagcaag gttgattttc tctgcaatta aatcaataaa gcgttgagga
    17821 ttgcggaaga cttcttgcaa gcggtcggat ttttgtaaaa ttttgcaaag ggtcgcacgc
    17881 gtcagcccgg ttttcttttg aatctcactc aataaatcag gtatcgccca ttgtgtattt
    17941 accgctgttt cattagatga gcgataatta gttgaaatcc ctgtttcatt catttccaat
    18001 ttcactttgg aatgcttaat ttttgtcgcc tcgatatcgg gcattttgcg gattttttcc
    18061 accgcacttt caatcaattc cgcattactg aaatctaccc gataagtggt ttggtgttta
    18121 atccgttccc aaattgccgc aaattccgga tgtaagctga aatctttacg gtagcgaacg
    18181 gcttttttat cttcccgttt tttaacattg cttttggcaa actgtacacc acaatcttcc
    18241 tcaatttcac gctgtaatgt ggcggcaaat tgttcgtaac tttcatttgc gataattgtc
    18301 agcgtattta gatgacgatc ataaatccgc aatccttgtt gattcacagg caaacgcaag
    18361 ccccgcccaa tctcttggcg ttttttcatt tcgcttgcag tatcgtttaa agtacaaatt
    18421 tgaaacacat tcggattatc ccatccttct tttaaggcag aatgcgagaa aataaaccgt
    18481 aacggattct ccagcgaaag caatttttct ttatcccgca taatcagttg ataagtctcg
    18541 ttatccgctt gcgttgtgcc attagtatct tttgctacgc cttttttatc ctgtgagaaa
    18601 taaccgttat gcacgccttg aggctcttct ttggctagct ccaaataaat ttcctcaaac
    18661 cattttgcaa attttccccc cgtagggctt tgtgcagaag ggcgataatt atccacttta
    18721 tcgataaaga acaaagacaa caccttgatc ccaagcggtc gtaatttctt ttctttttgc
    18781 aaatgctcgc caatagtgcg gcgaatctgc attttttgca catcttcttt aagttgggaa
    18841 tgttccgccc cttgaaaaat ttgtgtgccg ttggagaagc taaccatttg gctatcggta
    18901 tccattccgc ttaatataaa ccctgaacga tacatttcat tttggttgga aagctcatac
    18961 aaatcctgat tcggtttcac gctaatcact ttctttttca tcgctttttt atcgttaaat
    19021 aaaatttcga tttttgccga ccagcttttt gcccctgttt taatctcttt gagggaaaga
    19081 taagctccat ttacctcatt ttccgccaat acgctatcta cctcaatttg tttaactaat
    19141 cccatttcat aggcttgcac cggattgagg ctaaaaatcg ggttatacgc atttttatgg
    19201 gtggcagaat aacgcaaagt gaaaagcgga cttaaactct caatcgcatt acggcgaatc
    19261 tccgtttcca tattttgcgg ttcatcaata atcacaatcg gattcgcctg ttgaatatag
    19321 aaaatcggag catcaccgct ttcattcacg gtattaatca cgttactttc tttggtgaaa
    19381 gcatcaatat tcatcactaa aatttcaata tgattagcac tagcaaagga ttttaagcgt
    19441 gaagtttggt tacttttata ttcaaatttt tgattgacgc tcgggttatc aaataccgta
    19501 ttaaaatggc tttttgtagt ttccaacgta tgcaacactc cctcacgaat tgccacactc
    19561 ggcacgacaa tcacaaattt ttgccagccg tattgacggt ttaattcaaa aatcgtgcgt
    19621 aaatagacat aggttttccc tgtaccagtt tccatttcca cggtaaaatt ttttccctgt
    19681 tcgacaagca aagtgcggtc gattttttgc ctgttttgca gattagccaa attctgttgt
    19741 aactgctcat cactaatctg caagcctaaa ttcggcacaa accgcatatc attttgggct
    19801 ttgagtgaaa attcagttgc ggcattcggc tgtccgacaa acaaatttac tgtggcatta
    19861 acggcttgta attgatactc taaggtttca aattggagtt ccattttttc tccttaaatc
    19921 acaaaaaacg taatgccggc atcttgcatt tgcaattcgg tgtttttctt ccgggcatca
    19981 ttgccgttga ataaacggtc taaagcgatg acttttttcg gttgttgagc gataacagtt
    20041 tgaataattt cttcgtcggc tgcatttaac agcaaggcga aaatcagccc gttttcttcc
    20101 acccaaaaca ccttattttc caaccgcact tttgccgtga gttttaaccc taaacgcaat
    20161 aaaagctcat ataaaacggc ttgcgtatcc gctttttcac taacaggatc aataaacatt
    20221 tcaagttgat cggctaaatt ttcagatgtg ggcgattgcc attgtttaaa atggctttct
    20281 gtcagtttga agactttaaa acctacatca agcggtcggt tttcggggtt attttgcaaa
    20341 atctgctttc cggcacggcg aatacgttct tttgaaattt cagttatata tagtggtaaa
    20401 ttttttcttt ggcaaaattg ctttgctact tttccattct tactcttgat atcaatttct
    20461 tcaggatatt gaaccgcaat a
//
LOCUS       DANLRK010000022        19919 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.022, whole genome shotgun sequence.
ACCESSION   DANLRK010000022 DANLRK010000000
VERSION     DANLRK010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 19919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19919
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.022"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            <1..698
                     /gene="znuC"
                     /locus_tag="PXA33_000610"
     CDS             <1..698
                     /gene="znuC"
                     /locus_tag="PXA33_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HDL5119971.1"
                     /translation="QNINLSIYPNSITTIVGPNGGGKSTLLKILLKLLSPNKGKVIHQ
                     KGLKIGYVPQKLHLEPSMPITVYRFLALKPHTTKEMINQALDLFSITHLTENSMQKLS
                     GGELQRVLLARAILDRPQLLVLDEPMQGVDIIGQTELYQLLNKTRDLLNCAILMVSHD
                     LNIVMANTDEVLCINKHICCAGSPEVITHDPSFIHFFGDQFARNVAFYSHKHNHHHNL
                     HGDICLGECSCEH"
     gene            863..3676
                     /gene="sucA"
                     /locus_tag="PXA33_000611"
     CDS             863..3676
                     /gene="sucA"
                     /locus_tag="PXA33_000611"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HDL5119972.1"
                     /translation="MQYTHFDDWLASSALGGANQSYIEEIYEQYLENPANVDESWRNI
                     FDSFPKTPQVEQAHTPIRDYFRKLAREQQPQSVTIIDPEAGAKQVRLLQWINAHRFRG
                     YLEAKLDPINYYRWKTSSVPELDYKYHGFTDEDLNETFTVGRYVYDKDTMKLSELDKA
                     LKETYLGPIGLEFMHVQDIEQRNWLQAKMESVLNKPLFTKAEKLNLLSELTAADGLER
                     YLGAKFPGAKRFSLEGSDAFIPMMKEIIRHASRQGMTDVVLGMAHRGRLNMLVNVFGK
                     RPAELFDEFAGKHADESRTGDVKYHQGFSSNFAVDDKNVHLTLAFNPSHLEIVSPVVI
                     GSVRARQERMRDIEHEKVLAVTVHGDSAVAGQGVVQETLNMANVRGYKVGGTIRIVIN
                     NQIGFTTSNPNDTRSTEFCTDIAKMIQAPIIHVNGDDPEAVAFAARMAVEYRTKFKRD
                     IFIDLISYRRHGHNEADEPLATQPMMYSIIKKHPTPRKVYADRLITEGVLTNEQEIEM
                     LNLYRDALDNGECVVPEWREMDMAAVDWLQYLNYDWTALYESKFPEERFLTIAKRVCE
                     YPASLKAHSRVEKIYTDRKEMYEGKKLFDWGMAETMAYATLLDEGTHVRLSGEDAGRG
                     TFFHRHAVIHNQNDGTGYVPLTQLHANQGRFQVWDSVLSEEAVLAFEYGFATTDPKTL
                     TIWEAQFGDFANGAQIVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARVERY
                     LQLCAQQNIQVCVPSTPAQVYHMLRRQAIRKMRRPLVAITPKSLLRHPLAVSSLDELI
                     NGSFQNVIGELDNIDPKQVKRVVMCSGKVYYDLIEKRRENNQTDVAIIRIEQLYPFPL
                     EEMQQILEPYAHVKDFVWCQEEPLNQGAWYCSKHNFEASLPEDAKLTYAGRPASASPA
                     VGYPSLHAKQQKQLVEDALTIAE"
     gene            3779..5008
                     /gene="odhB"
                     /locus_tag="PXA33_000612"
     CDS             3779..5008
                     /gene="odhB"
                     /locus_tag="PXA33_000612"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005654196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HDL5119973.1"
                     /translation="MTIEILTPDLPESVADATVATWHKKVGDNVKRDEILVEIETDKV
                     VLEVPASSDGILAEITQEQGATVVSKQSLGKLVVAKAGDISSATIEQKTESTPSDRKH
                     AAIENSHANADDQGPAIRRLLAEHDLQAADIQGSGVGGRITREDIEKEIAKRVQINQV
                     KGSATSQNTVSTVAFNSRSEKRVPMTRLRKRIAERLLEAKNTTAMLTTFNELDMQPIM
                     NLRKTYGEKFEKQHGVRLGFMSFYIKAVVEALKRYPEINASIDGDDVVYHNYFDISIA
                     VSTPRGLVTPVLRDCDKMSMADIEKKIKELAEKGRDGKLTVEDLTGGNFTITNGGVFG
                     SLMSTPIINPPQSAILGMHAIKDRPVAIDGQVVIRPMMYLALSYDHRLIDGKESVGFL
                     VAVKELLEDPTRLLLEI"
     gene            5301..6461
                     /gene="sucC"
                     /locus_tag="PXA33_000613"
     CDS             5301..6461
                     /gene="sucC"
                     /locus_tag="PXA33_000613"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HDL5119974.1"
                     /translation="MNLHEYQAKQIFKQYGLPVSQGIACKTAEEAVEAIKQLQGEQWA
                     IKCQVHAGGRGKAGGVKLVRNEAEVREFADKWLGKRLVTFQTDANGQPVNTLYLEETC
                     NIDRELYLGAVVDRSAQKVIFMASSAGGMNIEEVAEKTPELIHKAVIDPLTGGQGYQG
                     RELAFKLGLKGDEVKQFADIFVKLANLFVEKDLALLEVNPLVITKEGQLLCLDAKMSI
                     DSNALYRHPELKELQDPSQDDEREAEAEKWNLNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLYGGKPANFLDVGGGATKERVAAAFKLILSDPNVKAVLVNIFGGIVRCDLIADGVIA
                     AVNEVGVNVPVIVRLEGTNAAQGREILANSGLSILAASSLKQAAELAVKSAN"
     gene            6470..7339
                     /gene="sucD"
                     /locus_tag="PXA33_000614"
     CDS             6470..7339
                     /gene="sucD"
                     /locus_tag="PXA33_000614"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HDL5119975.1"
                     /translation="MSILIDKNTKVICQGFTGGQGTFHSEQALAYGTQLVGGVSPNKG
                     GTTHLGLPVFNTVREAVEATGATATVIYVPAPGCKDAILEAIDAGIQLIVCITEGIPT
                     LDMLQVKQRLNQTGVRMIGPNCPGVITPDECKIGIMPGNIHKKGNIGIVSRSGTLTYE
                     AVKQTTDEGFGQSTCVGIGGDPIPGSNFIDILKLFQDDPETEAIVMIGEIGGSAEEEA
                     AAFIKENVTKPVVSYIAGVTAPKGKRMGHAGAIISGGKGTADEKFKILEDAGVTTVRS
                     LAEIGSALRKILG"
     gene            complement(7410..8855)
                     /locus_tag="PXA33_000615"
     CDS             complement(7410..8855)
                     /locus_tag="PXA33_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HDL5119976.1"
                     /translation="MDSIILFFESVLTWIVNTIDGPLWDVTIILLLGAGLFFTLTTGF
                     VQLRLLPQSLREMWGGRSTEGKSLTPFQAFTTGLASRVGVGNIGGVATAIALGGPGAV
                     FWMWITALMGMSSAFAESSLAQLYKTKDPNGMFRGGPAYYITRGLKLPSLAIAFAVTL
                     IFTFGFAFNAVQSNSIVEATKNAWDWNPVYVGMTLVVITGLIISGGVKRIGKVSAQVV
                     PMMALFYLIIAVIILGMNIEKVPSIISLIIESAFDFSAAAGGTFGALVSKTMLMGIKR
                     GLFSNEAGMGSAPNSAATSDAKHPASQGLIQMLGVFVDTIVVCTCTAVIILMSNNYGG
                     ETLRGISLTQSALQFHLGEFGLHFLAFVILLFCFTSIIGNYAYAETNIRYIKNSPWFV
                     WGFRAIVLFFVYFGAVRDGGIVWAFADTVMASMAVINLVAILILAPIVWIILKDYLRQ
                     VKSGKEPVFRIEEHQDLIHKGVDPLIWKSKE"
     gene            complement(8960..9946)
                     /locus_tag="PXA33_000616"
     CDS             complement(8960..9946)
                     /locus_tag="PXA33_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt transporter"
                     /protein_id="HDL5119977.1"
                     /translation="MRLKATYWGMIVIGFLSLFALYQLYPMILFKVMEWQKNFNLQLS
                     ASLNELAENQSKAGITLVFISFLYGVFHAVGPGHGKFILSSYLSFEKTKLNQAMKITF
                     ASALVQGLVAISLVTVIVVIFTLSRQYFNLTLKWVERGSFGLMILFGIYWCYQVYRTV
                     NRARKPIIKSIRISQKKAGKLPLVTQKGHIHTENCSCRHKHLPTSNEMQQVRDWKAQL
                     MLVLSIGSRPCSGAILVLFLSYTLNLYAWGVISALAMAVGTGLTLSLFAYLVIIARNK
                     AVKVSQWYFSAQINRNIVWILKFTLGLALILFGMVLFHSSFIDAGTGVFFKR"
     gene            complement(9937..10575)
                     /locus_tag="PXA33_000617"
     CDS             complement(9937..10575)
                     /locus_tag="PXA33_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1007 family protein"
                     /protein_id="HDL5119978.1"
                     /translation="MKFIKTIITLIALYSMPVFSHPHSFVDLKTNVIVEGSMLKSFQM
                     EWMLDEIASSELIYEVKNSQDKEKTQQNITAEMVQTALQNHYFSTLYDSQNQPIKFTT
                     KPTATRFEIKNNRVIFYITFHLAKPHELKQSKVRFYTYEPSYYIAMEYNRPADVNTSN
                     ASCKATLVQPQVDSKLRLYASGLDKNQSLDMPENGDYSLGAQFAQKVEIICD"
     gene            complement(10636..11014)
                     /gene="rnpB"
                     /locus_tag="PXA33_000618"
     ncRNA           complement(10636..11014)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PXA33_000618"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(11143..11430)
                     /gene="rplY"
                     /locus_tag="PXA33_000619"
     CDS             complement(11143..11430)
                     /gene="rplY"
                     /locus_tag="PXA33_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005625668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HDL5119979.1"
                     /translation="MSFKFEAEVRSAQGKGASRRLRHNGQVPAIIYGGNAEPVSIILD
                     HDKVNNAQVHDSFYNEVLTIVVAGKEEQVKVQAIQRHPTKPKLVHLDFKRA"
     gene            complement(11582..12850)
                     /locus_tag="PXA33_000620"
     CDS             complement(11582..12850)
                     /locus_tag="PXA33_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme"
                     /protein_id="HDL5119980.1"
                     /translation="MSDLKQQALDFHEFPIPGKISVTPTKALDTQHDLALAYSPGVAE
                     PCLAIEKDPSASYRYTARGNLVAVISNGTAVLGLGNIGALASKPVMEGKGVLFKKFAG
                     IDVFDIEINETDPEKLVEIIASLEPTFGGINLEDIKAPECFYVEQKLRERMGIPVFHD
                     DQHGTAIITGAAVINGLQVVGKAISEVRLVVNGAGASAIACTNLLRSLGVKKENITMC
                     DSKGVIFQGRGDKMDETKQFYAIEDNGWRTLADAVANADVFLGCSKGGALTQEMVKTM
                     AKDLLILALANPVPEITPAEAKAVRPDAIVCTGRSDFKNQVNNVLCFPFLFRGALDVG
                     AKTINEEMKMAAAHAIAELAKEPVPFEVISTYGELSFGPEYIIPTPFDPRLIVNVSIG
                     VAKAAIQSGVATRPITDWEAYAEQLKKLVA"
     gene            complement(13007..13918)
                     /locus_tag="PXA33_000621"
     CDS             complement(13007..13918)
                     /locus_tag="PXA33_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RimK family alpha-L-glutamate ligase"
                     /protein_id="HDL5119981.1"
                     /translation="MKWLMLCREPRLYSCCRIKAACEAKGIELDILDQNRMLLGLENG
                     EFKIYYQAGESYDNNRPEPVLLPNYDGILPRFGTGSTEMGCSVLRHFEAKGIPVLNHS
                     AAFALARDKWRTLQKLAKHNLPVPNTNFAGHLVSVKSQLNQFAFPLISKVLNGSQGNG
                     VMLFEGKNNAEAVLATFRQVNEPYLCQQFVGDAKGQDIRAFVIGNEVVAAMSRTSVTG
                     DFRANIHQGGTAQPIELTQMERALAIKATKTIGLDIAGVDFLRTEKGVVILEVNASPG
                     FEGIERVNDVDIASEMVSYFINKMKNA"
     gene            complement(13915..14634)
                     /gene="nfsA"
                     /locus_tag="PXA33_000622"
     CDS             complement(13915..14634)
                     /gene="nfsA"
                     /locus_tag="PXA33_000622"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NADPH nitroreductase"
                     /protein_id="HDL5119982.1"
                     /translation="MQSKPTLETILSHRSIRKFTEQPISEEIFQILIEAGKAASSSNH
                     LQCVTVIRVTDRQIRETLKAVSGMQYVVDCAEFLVFCIDFNKHKQLVPESQLEWAEVS
                     LIGAVDTGILAQNVFLAAESLGLGAVYIGALRNDIERVSKALNLPEYCVPLVGMCLGY
                     PAQEPAIKPRLPNSLVCYNNQYQDFDWTEFAKYNENLTAYYQARIGEPQQWQAAIHKT
                     LDKPVRPHILPFFNQKGLLKK"
     gene            complement(14646..15431)
                     /gene="mtgA"
                     /locus_tag="PXA33_000623"
     CDS             complement(14646..15431)
                     /gene="mtgA"
                     /locus_tag="PXA33_000623"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HDL5119983.1"
                     /translation="MEQSMAKRNKRFLSSLLKKIGLFFIPRKIKSKLGWLWFGSSRLL
                     TFGGTFFLIPILIFSVLPVPYSAYMVQKKVENLVQGKSYPIKKEWVSLNEISWQMQMA
                     VIASEDQKFESHFGIDLQAIETALKLNAKSKKVRGGSTISQQTVKNMFLWHGQSWLRK
                     GIEVPLTLLVENVWSKDRILEVYLNIAEFGNGIFGVEAAAKHFFKKSAKQLTLQESAL
                     LAASLPNPLVFRVDKPGPSMRKRQAWIMRQVSLLDGKNYLDKL"
     gene            complement(15406..15726)
                     /gene="trpR"
                     /locus_tag="PXA33_000624"
     CDS             complement(15406..15726)
                     /gene="trpR"
                     /locus_tag="PXA33_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HDL5119984.1"
                     /translation="MKTLYNQRDPNEWTQFLALLEQAVAEDKLEPFFSMFLTADERGS
                     LGLRLQIVQALLKGEDSQREIQQNLNTSAATITRGSNMLKTLDQDFLQWLNGKVNGTV
                     NGKA"
     gene            complement(15806..16447)
                     /gene="lolA"
                     /locus_tag="PXA33_000625"
     CDS             complement(15806..16447)
                     /gene="lolA"
                     /locus_tag="PXA33_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HDL5119985.1"
                     /translation="MKTLFKKSVFGLALFGLANVALADAQSVAELQRRLEQVSQYSAD
                     FDQTVRSSKGKQIQQGKGKFQVKRPNLFRMDTKSPQENLIVSDGKDLWFYDPFVSQVT
                     VNTVQDAVNNTPFVLLTSGDKSHWDQYDVSQSADTFVLKPKSKKSNLKQFDVRIDQSG
                     LLKGFSTIERDGQSNLYVLRNITTGGVSSDLFKFSVPKGAELDDQRGGKKSKK"
     gene            complement(16558..19416)
                     /gene="polA"
                     /locus_tag="PXA33_000626"
     CDS             complement(16558..19416)
                     /gene="polA"
                     /locus_tag="PXA33_000626"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005611716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HDL5119986.1"
                     /translation="MATIAKNPLVLVDGSSYLYRAFHAFPPLTNRLGEPTGAMYGVLN
                     MLKSLISQVNPSHIAVVFDAKGKTFRDELFKQYKSHRPPMPDDLRPQIEPLHRIIKAL
                     GIPLISIEGVEADDVIGTLAVQAAKDGKDVLISTGDKDMAQLVNDHIMLINTMNNTLL
                     DREGVIEKYGIPPELIIDFLALMGDSADNIPGVKGVGEKTALALLQGIGSLEQIYANL
                     DQIATLSFRGAKNFAPKLEAEKANADLSYLLATIKTDVELDVTHDQLLTQPQDRAELA
                     QLFEHYEFKRWLNEVNQNANPVTQTSAEKVPNNYQVTQAVRNEENVANVVEIDRSKYE
                     TVDTEQKLSAWIEKLTAARLIAVDTETDSLDAMSANLVGISFALENGEACYIPLGHKQ
                     KAQPQQADMFGDEERSESPSSDELVKNQLNLTACLSKLKPILLNPEIKKIGQNIKYDL
                     TIFANYGIELQGVAFDTMIESYTQNSTGRHNMDDLAARYLGHKTIPFEELAGKGKNQL
                     TFDKIEIAKAAEYAAEDADVTMKLHQVLWEKLQQEPELVKLFDEIEMPLVGVLSRIER
                     NGVLIDPKKLLTQSAEIEKRLEEVETLVHAEAGQTFNLASTKQLQEILFEKLGLPVLK
                     KTPKGAPSTNEEVLDELAQQGHLVPKLLMEHRGLSKLKSTYTDKLPQLINKKTGRVHT
                     SYNQAVTATGRLSSSDPNLQNIPIRNEEGRRIRQAFVAREGYKIVAADYSQIELRIMA
                     HLSNDEGMINAFAEGKDIHRATAAEIFGVELGEVTSEQRRSAKAINFGLIYGMSSFGL
                     SNQLGIGRAEAQKYMDLYFQRYPAVQQFMTDIREVAVEKGYVETLFGRRLYLPDIKSG
                     NAILRKAAERVAINAPMQGTAADIIKVAMIGIDNAIRDNDEIKMIMQVHDELVFEVKA
                     DKIDHYSQLIKTEMEKAISLKVPLIAEVGVGENWDEAH"
     gene            complement(19701..>19919)
                     /locus_tag="PXA33_000627"
     CDS             complement(19701..>19919)
                     /locus_tag="PXA33_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5119987.1"
                     /translation="LNAKYVVGTGDDTDGEVTNFVIITSEYKYIDAQSAKAITDAPLT
                     KEDIGKTPTQIMLGRIYKYLKETGQIVV"
BASE COUNT         5942 a         4401 c         3860 g         5716 t
ORIGIN      
        1 tacaaaatat taatttatct atttatccta actctatcac gacgatagtc gggccaaatg
       61 gtggaggaaa atctaccctg cttaaaattt tgctaaaatt acttagccca aataaaggaa
      121 aggttattca tcaaaaagga ctcaaaattg gctatgtacc acaaaaattg catttagaac
      181 cctcgatgcc cattactgtt tatcgatttc ttgcgctaaa accacatact accaaagaaa
      241 tgattaacca agcactagat ctgttttcta ttacccatct cactgaaaat agtatgcaaa
      301 aattatcggg tggtgaactt caacgtgtat tgctggctcg ggcaatttta gatcgtccgc
      361 aactcttagt gttagatgaa ccaatgcaag gcgttgatat tataggccaa accgaactct
      421 atcaattact taataaaaca agggatctcc ttaactgtgc tattttaatg gtctcacacg
      481 acttaaatat tgtgatggcg aatacagatg aagtgctttg catcaataaa catatttgtt
      541 gtgccggaag ccccgaagtc attacacacg atccgagctt tatccatttt tttggcgacc
      601 aatttgcacg taatgttgca ttttattcac ataaacataa ccatcaccat aatttacatg
      661 gcgatatctg ccttggtgaa tgtagttgtg agcactgata ggcgtccttt ttaccaaatg
      721 tttaaaattt gtaaaaataa tgctaaaaat aaccgcttgc agcacaagat ttatctaaaa
      781 atcagactta cttcacattt tatttgctta aattggagta taataagggc gtgatttcat
      841 accacaaaca ggtcaggtct ttatgcaata tacacatttt gatgattggc ttgcatcatc
      901 tgcattaggc ggtgcaaacc aatcttatat agaagaaatt tatgaacagt atctagaaaa
      961 tccagcaaat gttgatgaaa gctggagaaa catctttgat tcatttccta aaactcccca
     1021 agttgaacaa gctcacaccc ctattcgtga ctatttccgt aaattagccc gtgaacagca
     1081 acctcaatcc gtcactatta ttgacccaga agcaggggcg aaacaagttc gcttactgca
     1141 atggattaac gctcaccgtt tccgtggcta tttagaggca aaactagacc caattaacta
     1201 ttaccgttgg aaaacctcca gcgtgcctga attggactac aaatatcacg gcttcaccga
     1261 tgaagatctc aacgaaactt ttacggtcgg tcgctatgtc tatgataaag acacaatgaa
     1321 gctcagcgaa ctggataaag ccctaaaaga aacttattta gggcctattg gtttagaatt
     1381 tatgcacgtt caagatattg aacaacgcaa ctggcttcaa gccaaaatgg aaagcgtgct
     1441 aaacaaaccg ctgttcacca aagcagaaaa actgaattta ttaagcgaat tgaccgctgc
     1501 cgatggttta gagcgttact taggggcaaa attcccgggg gcaaaacggt tctcattgga
     1561 aggtagtgat gcctttattc cgatgatgaa agaaattatc cgccacgctt cccgtcaagg
     1621 tatgaccgat gtggttttag gtatggcaca ccgtggtcgt ttgaatatgt tagtgaacgt
     1681 atttggtaaa cgcccggcag aattgtttga tgaatttgcc ggcaaacacg ctgatgaaag
     1741 ccgcacgggc gatgtgaaat accaccaagg tttctcctca aactttgccg tagatgataa
     1801 aaacgttcac cttacccttg ctttcaaccc atcgcattta gagattgtta gcccggtggt
     1861 aatcggctca gtgcgtgcaa gacaagaacg tatgcgtgat atcgaacacg aaaaggtgct
     1921 tgcggtgacc gttcacggtg actctgcagt ggcagggcaa ggtgtagtgc aagaaacctt
     1981 aaatatggca aatgttcgtg gctataaagt aggaggcact atccgtattg tgatcaacaa
     2041 ccaaatcgga tttaccacct ctaacccaaa tgacacccgc tccaccgaat tttgtaccga
     2101 cattgccaaa atgattcagg caccgattat tcacgttaat ggtgatgatc cggaagctgt
     2161 tgcctttgca gcgagaatgg ctgtggaata tcgtactaaa tttaaacgtg atatttttat
     2221 tgatttaatc tcttaccgtc gtcacggtca taatgaagcc gatgagcctt tagcaactca
     2281 gccaatgatg tatagcatca tcaaaaaaca cccgacacct cgcaaagtct atgcggatcg
     2341 tttaattacc gaaggtgttt taaccaacga gcaagaaatc gagatgttaa acctgtatcg
     2401 tgatgcctta gacaacggcg aatgcgttgt gccggaatgg cgtgaaatgg atatggcagc
     2461 ggtcgattgg ctacaatatc tcaattacga ctggactgcc ctatacgaaa gtaaattccc
     2521 tgaagaacga ttcttaacca ttgccaaacg ggtgtgcgaa tatccggctt cgcttaaagc
     2581 tcattctcgg gttgaaaaga tctacactga tcgcaaagag atgtatgaag gcaagaaatt
     2641 attcgactgg ggtatggctg aaacaatggc atacgctact ttgttagacg aaggcacaca
     2701 tgttcgcctt tcaggagaag atgccggacg tggcactttc ttccatcgcc atgcggtaat
     2761 ccataaccaa aatgatggaa caggctatgt gccacttact caactgcacg ccaatcaagg
     2821 gcgtttccaa gtatgggatt ccgtactctc tgaagaagcg gtactcgcct ttgaatatgg
     2881 ctttgccact accgacccga aaacactgac catttgggaa gctcaatttg gagatttcgc
     2941 taacggagct caaattgtta tcgaccaatt tatcagctca ggcgaacaaa aatggggcag
     3001 aatgtgcggt ttagtgatgt tattaccaca cggctatgaa ggtcaagggc cggaacactc
     3061 ctctgcccga gtggaacgct atttacagct ttgtgctcag caaaatattc aagtttgcgt
     3121 gccatcaaca ccggcacagg tttaccatat gttacgccgt caagcgatcc gcaaaatgcg
     3181 tcgcccattg gttgcaatca caccaaaatc attgctacgc cacccacttg cggtgtcatc
     3241 tcttgatgaa ttgattaacg gttcattcca aaatgtgatt ggcgagctcg ataatatcga
     3301 tcctaaacaa gtcaaacgtg tggtgatgtg ttcgggtaaa gtgtactacg acttaattga
     3361 aaaacgccgt gaaaataacc aaacggacgt tgccattatt cgtatcgaac agctctaccc
     3421 gttcccactt gaagaaatgc aacaaattct cgagccttac gctcacgtta aagactttgt
     3481 ctggtgtcaa gaagagccac taaaccaagg tgcgtggtat tgcagcaagc acaattttga
     3541 ggcttctctg ccggaagatg caaaattaac ttacgcaggc agaccggctt ctgcttcacc
     3601 ggcggtgggt tatccatcgt tacacgcaaa acaacaaaaa caacttgttg aagatgcttt
     3661 aacgatcgct gaataataaa atgggcgtat atacgcccta caagcggttc acttttaacc
     3721 aaattttaca atttacgctg tcggagtttt aaaactctct cgaaaaaagg aataagttat
     3781 gaccattgaa attttaaccc ccgatttacc cgaatctgtt gccgatgcga ccgttgccac
     3841 ttggcataaa aaagtgggcg acaatgtaaa acgtgatgaa attttagtcg aaattgaaac
     3901 agacaaagtc gttttagaag taccggcttc aagcgatggt attcttgcag aaattaccca
     3961 agaacaaggt gctactgttg tatccaagca atctctgggt aaattagtag tagctaaagc
     4021 cggtgatatt agctctgcaa ctattgagca aaaaacagaa tccacgccat cagaccgcaa
     4081 acacgcagca attgaaaaca gccacgcgaa cgctgatgat caagggcctg cgattcgccg
     4141 tttattagcc gaacacgact tacaagccgc tgatattcaa ggcagtggtg taggcggcag
     4201 aatcacccgc gaagatattg aaaaagagat tgccaaacga gtgcaaatca accaagtaaa
     4261 agggtctgca accagccaaa acaccgttag cactgtagca tttaacagcc gtagcgaaaa
     4321 acgtgtgccg atgacacgac tccgcaaacg tattgccgaa cgcttactag aggcaaaaaa
     4381 caccactgca atgctcacta cttttaacga gctggatatg caaccaatta tgaatttacg
     4441 caaaacctat ggcgaaaaat tcgaaaaaca acacggtgtg cgtttaggct ttatgtcgtt
     4501 ctacattaaa gccgtggttg aagcattaaa acgctaccct gaaatcaatg cctccattga
     4561 tggcgatgat gtggtttatc ataactattt cgacatcagc attgcggttt ctaccccacg
     4621 aggcttagtc acacctgtgt tgcgtgattg cgacaaaatg agtatggcag atattgagaa
     4681 gaaaattaaa gaacttgccg aaaaagggcg tgacggcaaa ttaaccgttg aagacttaac
     4741 cggcggtaac tttaccatca ccaacggcgg ggtattcggt tcattaatgt ccaccccaat
     4801 tatcaacccg ccacaaagtg cgattttggg tatgcacgct attaaagacc gccctgtcgc
     4861 cattgacgga caagtagtga tccgcccgat gatgtaccta gccttaagct atgaccaccg
     4921 tttaattgac ggtaaagaat ctgtcggttt cttagtggca gttaaagagt tattagaaga
     4981 cccaacccgt cttcttttgg aaatttaatc gaaaatatga ccgcttgtac ttttagctac
     5041 aagcggtctg tttttaataa aatttgacaa aatccgataa acgctctatt tgagttccct
     5101 tctgagccac ctgagcgact ttgaatttgt aggaaaaatt tagtgtttga gcttacaagg
     5161 caagcgagtt ataaattttt ccgtcaagca aattcaaact aagcgaagaa agtggcgaag
     5221 cagggtgtgc tttcttttgg ttacttttct ttgcacaagc aaagaaaagt aacataaaaa
     5281 aaaccaaacg aggaaactct atgaatctac acgaatatca agcaaaacaa atattcaaac
     5341 aatacgggct tcccgtaagc caagggattg cttgcaaaac ggcagaggaa gccgttgagg
     5401 caattaaaca actacaaggt gaacaatggg cgataaagtg ccaagttcac gctggtgggc
     5461 gtggcaaagc cggcggggtg aaactggttc gcaatgaagc cgaagtgcgt gaatttgccg
     5521 ataaatggct cggcaaacgc ttagttacct tccaaaccga tgctaatggg caaccggtta
     5581 ataccttata tttagaagaa acctgcaata tcgaccgtga attgtactta ggtgcagtgg
     5641 ttgatcgctc cgctcaaaaa gtgattttta tggcatcaag tgccggtggt atgaatattg
     5701 aagaagtagc ggagaaaacc cctgaattaa ttcacaaagc ggtaattgac ccactcaccg
     5761 gtgggcaggg ttatcaaggg cgtgaattag catttaaatt aggcttgaaa ggtgatgaag
     5821 ttaaacaatt tgcggatatt ttcgtgaaac tggctaacct atttgttgaa aaagatttag
     5881 ctttattaga ggtaaatcca cttgtcatca ctaaagaagg gcaactactc tgcttagatg
     5941 caaaaatgtc gattgacagc aatgctctct accgccaccc agaacttaaa gagttgcaag
     6001 acccatcgca agatgacgaa cgtgaagctg aagcggaaaa atggaatctc aactacgttg
     6061 cactagatgg caatatcggc tgtatggtaa acggtgcggg gctggcaatg gggacgatgg
     6121 atatcgtcaa actttatggt ggtaaaccgg ctaacttctt agacgttggc ggtggtgcaa
     6181 ccaaagagcg tgtagcagca gcttttaaat tgattttatc tgatccgaat gtgaaagccg
     6241 tgttagtcaa tatttttggt ggtatcgtcc gctgcgattt gattgctgat ggcgtcattg
     6301 ctgctgtaaa cgaagtcggc gtgaatgtgc ctgttattgt gcgtttagaa ggcactaatg
     6361 ccgcacaggg gcgtgagatt ttagccaata gcgggctgag tattctagcg gcatcatcat
     6421 taaaacaagc ggctgaatta gcggtaaaat ctgcaaatta ggaggaaaaa tgtcgatttt
     6481 aattgataaa aacacaaaag taatttgcca aggtttcacc ggcggacaag gtactttcca
     6541 cagcgaacaa gcccttgctt atggcacaca attagtcggc ggagtttcgc cgaataaagg
     6601 tggcacaacc catttaggct taccggtttt caacaccgta cgcgaagcgg tagaagcaac
     6661 cggtgcaact gcaaccgtta tctacgttcc ggctcccggc tgtaaagatg ctattttaga
     6721 agccattgat gccggcatcc aactaattgt ctgtattact gaaggcattc caaccttgga
     6781 tatgctacaa gttaaacaac gtctaaacca aaccggcgtg cgaatgatcg gtccgaactg
     6841 cccgggtgtg attacccctg atgagtgcaa aatcggcatt atgccgggca atatccacaa
     6901 aaaaggcaat atcggtattg tttctcgttc gggtacttta acctatgaag cagttaaaca
     6961 aacaactgac gaaggctttg gtcaatctac ttgtgtcggt atcggtggtg atccgattcc
     7021 gggatctaac tttattgaca ttttaaaact gttccaagac gatcctgaaa ctgaagcaat
     7081 tgtgatgatt ggtgaaatcg gcggctctgc cgaagaagaa gcggcagctt tcatcaaaga
     7141 aaatgtcacc aagcctgtag tcagctatat tgccggtgtc accgcaccaa aaggcaaacg
     7201 aatgggacac gccggtgcga ttatcagcgg cggcaaagga accgccgatg aaaaattcaa
     7261 aatccttgaa gatgccggtg tgacaaccgt cagaagttta gccgaaatcg gttcagctct
     7321 tagaaaaatt ttaggctaaa ctaaaagttc aagcagtcat atttacccat ttttttgtaa
     7381 atatgaccgc ttgttttttt gctattaaat tactctttcg atttccaaat taatgggtca
     7441 acacctttat ggataaggtc ttgatgttct tcaattctaa acactggctc tttgccactt
     7501 ttcacttggc gaagatagtc ttttaaaata atccagacaa tcggagcaag aattaagata
     7561 gccaccaagt taattaccgc cattgatgcc atcactgtat cggcaaatgc ccatacaatc
     7621 cctccgtctc ttactgcacc gaagtacaca aagaataaaa cgatggcacg gaatccccac
     7681 acaaaccacg gactgttttt aatgtagcgg atattagttt ctgcataagc atagttaccg
     7741 ataatggatg tgaagcaaaa cagtaaaatc acaaatgcta aaaagtgtaa accaaattca
     7801 cctaaatgga attgtaacgc actttgagta agtgaaatac ctcttaaagt ttcaccacca
     7861 tagttatttg acataagaat gatcactgca gtacaagtac agactacaat tgtatctaca
     7921 aatacgccca acatttgaat taagccttga cttgccgggt gtttcgcatc agaagtcgct
     7981 gcagaatttg gtgcagatcc cataccagct tcgtttgaga ataagccacg tttaatcccc
     8041 attagcatag ttttggatac caatgctcca aacgttccac ctgcagcagc agaaaaatca
     8101 aaggcgcttt caataattaa agagattata cttggcactt tttcaatatt catacctaaa
     8161 ataattactg caataatcaa gtagaataat gccatcattg gaacaacttg ggcagaaact
     8221 ttaccaatac gcttgacccc tccagaaata attaaccctg taatcaccac taatgtcatg
     8281 cctacgtaga ctgggttcca atcccacgca tttttggttg cttccacaat agagttagat
     8341 tgcacagcat taaaagcaaa accaaaggtg aagataagcg tcactgcaaa agcaatagca
     8401 agagaaggca gttttaaacc acgagtgatg taataggctg gcccaccacg aaacattccg
     8461 tttggatctt tggttttata caactgagcc agtgaagact cagcaaaggc ggaactcatt
     8521 cccattaagg cggtaatcca catccagaat accgcacccg gcccacccaa agcgattgca
     8581 gtagctaccc caccaatatt accaacccca acacggctgg ctaatccagt agtaaaggct
     8641 tggaacggag ttaatgattt cccttccgta gaacgtcctc cccacatttc acgtaaactt
     8701 tgcggaagaa gacgcagctg gacaaaacct gttgtaagag tgaaaaataa ccctgcaccg
     8761 agcaatagga taatcgttac atcccaaagt ggaccgtcga ttgtgttcac aatccaagtc
     8821 agtacactct caaaaaatag aataattgaa tccataaaga cctcttaatt gaaaataaaa
     8881 cttccacatt gtaaccgatt taatattttt aaaaagtttt tttaatactt ttaataaaaa
     8941 tttttaataa cttattaatt tagcgtttga aaaaaacgcc tgtaccagca tcaatgaaac
     9001 tactgtgaaa taataccatt ccaaatagaa ttaatgcgag tccaagtgta aattttaata
     9061 tccaaacgat atttcgatta atttgagctg aaaaatacca ttggctaacc ttaactgcct
     9121 tatttctcgc aatgataaca agataagcaa ataagcttaa agtcagccct gttcctactg
     9181 ccatagctaa tgctgaaata accccccaag catagagatt gagcgtataa gaaagaaata
     9241 aaacaagaat agcgccagag caaggtcttg agccaatact taataccaac atcagctgtg
     9301 ctttccaatc tctcacttgt tgcatttcat ttgaagtcgg taaatgttta tgcctacagc
     9361 tacaattttc ggtatgaata tgccctttct gcgtgacaag cggtaatttt ccagcttttt
     9421 tttgcgaaat acgaatggat ttaatgatag gctttcgcgc tctgttcacc gttcgataca
     9481 cctggtaaca ccagtaaatg ccgaacaaga tcatcaaacc aaagctcccc cgctcaaccc
     9541 attttagggt taaattaaaa tattggcgag agagagtaaa aatcaccaca atcaccgtca
     9601 ccaatgaaat tgccactaag ccctgcacca atgctgaggc aaaagtgatt ttcatcgcct
     9661 gatttaattt tgttttttca aaagaaagat aactgctcag aataaatttt ccgtgaccag
     9721 gcccaactgc gtgaaatact ccatataaaa aactaataaa tactaaggta ataccggctt
     9781 tactttgatt ttcagctaat tcattgagcg aagcggaaag ctgtaaatta aagttttttt
     9841 gccattccat cactttaaac aatatcattg gataaagctg ataaagagca aataggctca
     9901 aaaatcctat cactatcatt ccccaatatg ttgcttttaa tcgcatataa tctccacttt
     9961 ttgagcaaat tgggcaccaa gagagtaatc tccattttca ggcatatcca gtgattggtt
    10021 tttatccaaa ccggaagcat ataagcgtaa cttactatct acttgaggtt gcaccaatgt
    10081 tgctttacaa cttgcattac tggtattgac atcagcaggg cgattatatt ccatcgcaat
    10141 ataataactc ggttcataag tatagaatct cactttactc tgctttaatt cgtgaggttt
    10201 agctaaatga aaagtaatat aaaaaatcac tctgttattc ttgatttcaa agcgagtcgc
    10261 tgtgggcttt gtcgtaaatt ttatcggctg attttgacta tcatataagg tgctaaaata
    10321 atgattttgc aatgcggttt gcaccatttc tgcagtgata ttctgctgag ttttttcttt
    10381 atcttggcta tttttcactt cataaattaa ctcagaagaa gcaatttcat ctagcatcca
    10441 ttccatttgg aaagatttca acattgagcc ttccacaata acattcgttt tcaaatcaac
    10501 aaatgaatgt gggtgagaaa aaactggcat tgaatataat gcaattaacg tgataatcgt
    10561 cttaataaat ttcatattta ctttcactat aaaataaaaa agcgaacaag ttccccctgt
    10621 tcgcatttca agaatttagt gagttggtcg ataagccgag ttctgtcgtg gacaatcatt
    10681 cctctagacg tacatttaca catacgctca agcaacctac ccgaactctc agcgggccac
    10741 cgattgagtt cctatttggt cttgctacga gtggagttta ccctgctgtg gaatgttacc
    10801 acccacacgg tgcgctctta ccgcaccctt tcacccttac cgacaaagcc ggcggtctgc
    10861 tctctgttgc actggtcgta ggctcacgcc ccccggacgt tatccggcac tctgccctgt
    10921 gtagctcgga ctttcctctc gtttacttgt cccccagttt acgttttcac gaatttagag
    10981 gcttcaataa accagcgatt gcctaaccaa ctcagcacta gagtataatg ctttcgcaaa
    11041 aaattgcaag gaaataaccg cttgtagtta ttatggcaaa ataaaaaagg attaagttca
    11101 tttaaaaact taatcctctt cgctttaatt cactcgtaaa aattacgcac gtttgaagtc
    11161 taagtgaact aattttggtt tagttgggtg gcgttgaatt gcttgtactt ttacttgctc
    11221 ttctttacct gcaacaacga tagttaatac ttcgttatag aatgaatcgt gaacttgtgc
    11281 gttgttcact ttatcgtgat ctaaaatgat tgatacaggc tctgcattac caccgtagat
    11341 gattgcagga acttgaccat tgtgacgcag gcggcggctc gcacccttac cttgcgcaga
    11401 acgaacttca gcttcaaatt tgaatgacat agttttattc tcttaaataa aatggggaaa
    11461 tccccgtgaa tagaaaaaac agcaggcgac ccagctgttt ccgaaaaaat atcggcatat
    11521 ccgtctcgat atgccaacta cgtattttac tgaagttgca gaaaaatgca atgtaaaaat
    11581 attaagcaac cagttttttc aactgctctg cataagcttc ccaatctgta attggtcgag
    11641 ttgctacacc tgattgaatt gctgcttttg ctacaccaat cgagacattc acgatcaaac
    11701 gtggatcaaa tggcgttgga ataatatatt ccgggccaaa cgataattcg ccataagttg
    11761 aaataacttc aaacggcaca ggttctttag ccaactctgc aatcgcatgg gctgctgcca
    11821 ttttcatttc ttcattaatg gtttttgccc caacatctaa tgctccacgg aataagaacg
    11881 ggaaacaaag cacattattc acttggtttt taaagtctga acgaccggta catacaatcg
    11941 catctggacg gacagccttc gcttcagcag gtgtaatttc cggcacaggg ttcgctaaag
    12001 caagaattaa tagatctttt gccattgttt taaccatttc ttgcgttaaa gctccgcctt
    12061 ttgaacaacc taagaaaaca tctgcattcg caacggcatc tgccaatgtt ctccaaccat
    12121 tatcttctat tgcatagaat tgtttggttt catccatctt atcaccacga ccttggaaaa
    12181 taacgccctt agaatcgcac attgtgatat tttctttttt cacacctaat gaacggagta
    12241 agttagtaca cgcaatcgct gatgctccag caccgtttac cactaaacgt acttctgaaa
    12301 ttgctttacc taccacttgt aatccgttaa tcaccgccgc accggtaata attgccgtac
    12361 cgtgttgatc atcgtggaac acagggattc ccatacgttc acgtaatttt tgctctacat
    12421 aaaaacattc aggtgcttta atatcttcca gattaatacc accaaaagtc ggttctaacg
    12481 atgcaataat ctccactaat ttctccggat cagtttcatt aatttcaata tcaaacacat
    12541 caattccggc aaatttctta aatagaacac ctttgccttc catcacaggc ttacttgcta
    12601 aagcaccaat attgcctaag cctaacactg ctgtaccatt agaaattact gcaactaagt
    12661 tacctcgtgc cgtataacga taagaagctg aaggatcttt ttcaatcgct aagcaaggct
    12721 cagcaacccc aggagagtac gctaaagcta aatcgtgctg agtatctaat gctttagtag
    12781 gagtaacgga aatttttccg gggattggga actcgtgaaa atcaagcgcc tgttgtttta
    12841 aatcgctcat tttagaaacc ttataatcta taaaaataaa agtaatcaat aatagccaaa
    12901 gagtagccat tttaccctaa attttttcgc atttttatgg ctttgctctc attttttatg
    12961 atatttacaa aaaataatac aaaaacgtcc gcttgtttta ctcaaatcaa gcattcttca
    13021 tcttattgat aaaataagaa accatttcag aagctatatc aacatcattt actctctcta
    13081 tgccttcaaa tcctgggctg gcattgactt ctaaaataac tacgcctttc tctgtccgca
    13141 gaaaatcaac gcctgcaata tcaagcccta tcgttttagt tgctttaatt gctaacgcac
    13201 gttccatctg agttaattca atcggttgag ccgttccacc ttgatgaata ttggctctaa
    13261 aatcgcctgt tacgctggtt cggctcatgg cagcaaccac ttcattgcca atcacaaacg
    13321 cccgaatatc ttgcccttta gcgtcgccca caaattgttg gcatagataa ggctcattta
    13381 cttgcctaaa ggtcgctaac acggcctcgg cattattttt gccttcaaat agcattacgc
    13441 cattcccctg tgaaccattt agcacttttg aaataagtgg aaaagcaaat tgattgagtt
    13501 gtgatttcac agaaaccaaa tgccctgcaa aattcgtatt aggaaccggc aaattgtgtt
    13561 tggctaattt ttgtaatgtt cgccatttgt ctcgggctaa cgcaaatgcc gctgaatgat
    13621 ttagaacagg aatacctttc gcctcaaaat ggcgtaacac cgaacagccc atttcggtac
    13681 tgcctgtgcc aaaacggggt aaaatgccgt cataattcgg caacaatacc ggctccggac
    13741 ggttgttatc atagctctcg ccagcttgat agtagatctt aaattcgcca ttttctaacc
    13801 cgagtagcat tctattctgg tctaaaatat ccagctcaat cccctttgcc tcacaagcgg
    13861 cttttattcg acagcagcta tataaacgag gctctcgaca tagcattagc cacttcattt
    13921 tttcagcaat cctttctgat tgaaaaacgg caaaatatgc ggacgaacag gcttatccaa
    13981 tgttttatga atagctgctt gccattgttg tggctcgccg attctggctt ggtagtaagc
    14041 ggtcaaattt tcattatatt ttgcaaattc agtccaatca aaatcctgat attgattgtt
    14101 ataacaaacc aaactattcg gcaaacgagg ttttattgcc ggctcttgtg ccggataccc
    14161 caaacacata cccaccagtg gaacacaata ttccggcaag ttaagggctt tagaaacccg
    14221 ctcaatatca ttacgcaatg ctccaatata caccgctccc agccctagtg attcggcggc
    14281 taaaaataca ttctgggcca aaataccggt atctaccgcc ccaatcaacg acacctccgc
    14341 ccattccaac tgcgattcag ggacaagttg tttgtgttta ttaaaatcaa tgcagaacac
    14401 caaaaattca gcacaatcta ctacatattg catacctgaa acggctttta atgtttctcg
    14461 aatctgccga tcggttacac gaatcaccgt cacacattgc agatgatttg aacttgaagc
    14521 cgctttacct gcctcaatta agatttggaa aatctcttca gaaataggct gttcggtaaa
    14581 tttacgaata gaacggtgag ataaaatcgt ctcaagagtc ggtttacttt gcatattttt
    14641 ctcgtctaaa gtttatctaa ataatttttg ccatcaagca aacttacttg tcgcataatc
    14701 cacgcttggc gttttcgcat actcgggccg ggtttatcta ccctaaacac caacgggtta
    14761 ggcaaagagg ctgccaatag tgcggattct tgcaaggtta gctgtttggc tgattttttg
    14821 aaaaaatgct ttgcggctgc ttccaccccg aaaatgccgt taccaaattc cgcaatattg
    14881 aggtacacct ctaaaatccg atccttactc cacacatttt cgactaacag ggttaaaggc
    14941 acttcaatcc ctttgcgtaa ccagctttgc ccgtgccaca aaaacatatt ttttaccgtt
    15001 tgttgcgaaa tggttgaacc tccacgcact tttttggatt tagcattcag tttcaaggca
    15061 gtttctatcg cttgtaagtc aatgccaaaa tggctttcaa acttttgatc ttctgaggca
    15121 atcacagcca tttgcatttg ccacgaaatc tcattcaggc tcacccattc ttttttgatc
    15181 gggtaagatt tcccctgaac cagattctcc accttttttt gcaccatata cgcagaataa
    15241 ggcaccggta acaccgaaaa aatcagtatc ggaatcagga aaaatgtgcc accaaaggtt
    15301 aataagcgac tagaaccaaa ccaaagccag cccagtttac tttttatttt gcgaggaata
    15361 aaaaaaagcc ctattttctt caaaagagag cttaaaaatc gcttgttacg ctttgccatt
    15421 gactgttcca ttaaccttgc cgttcagcca ttgcagaaaa tcttggtcga gtgttttaag
    15481 catatttgaa ccacgagtga tggttgccgc actggtattt aaattttgtt ggatttcccg
    15541 ttgcgaatct tcacctttta gcaaagcttg cacaatttgt aaacgcaagc ctaatgaacc
    15601 tcgctcatcg gcggtgagaa acattgagaa aaacggctca agtttatctt ccgcaaccgc
    15661 ttgttcaagc agagccaaaa actgcgtcca ctcgtttgga tctcgctggt tatagagtgt
    15721 tttcatactg tcccttgaag taaaaacggt gacaagcggt cagattttta caaaattttg
    15781 cagaaaaacg accgcttgct ctccattatt ttttcgattt tttaccgcca cgttggtcgt
    15841 ctaactctgc ccctttcggt acgctgaatt taaacaaatc gcttgagaca ccgccggtag
    15901 taatattgcg taacacatat aagttacttt gcccgtcacg ctcaatggta ctgaaacctt
    15961 ttaataaacc gctttggtca atccgcacat caaactgttt taaattgctt tttttcgatt
    16021 ttggcttaag cacgaaagta tcagcactct ggctgacatc atattgatcc cagtggcttt
    16081 tatcaccgct ggttaataag acgaatggcg tattattaac tgcatcttgc acggtattta
    16141 ccgtaacttg tgaaacgaaa ggatcgtaaa accataaatc tttgccgtca gatacaatca
    16201 aattttcttg tggtgatttg gtatccattc taaataagtt cgggcgtttt acttggaatt
    16261 tgcctttgcc ttgctgaatt tgtttacctt tgctagaacg cacggtttgg tcgaaatccg
    16321 cactatattg gcttacttgt tccaaacggc gttgtaattc tgcaacagat tgagcatcag
    16381 ccaatgccac atttgccaag ccaaataacg ctaaaccgaa taccgatttt ttaaataatg
    16441 ttttcatttg ttttcctttt taccaacaag aattttcttg tttgacaata aaatcatcaa
    16501 aagtttcata ttaatgaact gttacaacaa tacaataaaa gtcgtgaatt gtatagttta
    16561 atgagcttcg tcccagtttt cgcctacgcc aacttcggca atcagtggca ctttgaggct
    16621 aatcgctttt tccatttcgg ttttgattaa ttggctgtaa tggtcgattt tatcggcttt
    16681 gacttcaaac accaattcat cgtgaacctg cataatcatt ttgatttcat cattatcacg
    16741 aatcgcatta tcaataccaa tcatcgccac cttaataata tcggcagccg tgccttgcat
    16801 tggggcgttg attgccaccc gttctgctgc tttacgcaaa atagcattgc cggatttaat
    16861 atcaggcaag tacaaacggc gaccaaatag ggtttcaaca tagccttttt caactgcaac
    16921 ttcacgaata tcggtcataa attgttgaac cgccgggtaa cgttggaagt agagatccat
    16981 atatttttga gcctctgcac gcccaatccc taattggttt gaaagcccaa atgacgacat
    17041 tccgtaaatc agcccgaagt taattgcttt cgcactgcgg cgttgttcgg aggtgacttc
    17101 acctaactcc accccgaaaa tttcggcagc ggtagcacgg tgaatatctt tgccctccgc
    17161 aaaggcgtta atcattccct catcattcga taaatgagcc ataatccgta gctcaatttg
    17221 tgaatagtcg gcagcgacaa ttttataacc ttctctcgcg acaaaagctt ggcgaatccg
    17281 tctgccctct tcattgcgga tcggaatgtt ctgcaaattc ggatcgctgg aagaaaggcg
    17341 tccggttgcg gtgactgctt gattgtaaga ggtatgcacc cgccctgttt ttttgttaat
    17401 cagttgtggt aatttatcgg tgtaggtaga tttcaactta cttaacccac gatgctccat
    17461 taataacttc ggcactaaat gcccttgttg agccaactcg tctaacactt cttcgttggt
    17521 tgagggtgca ccttttggag ttttcttcaa caccggcaag cctaattttt caaacaaaat
    17581 ctcttgtaat tgtttagttg aggcaagatt gaaggtttgc cctgcttctg cgtgtactaa
    17641 tgtttcaact tcctccaaac gtttttcaat ttcagccgat tgggtcaata attttttcgg
    17701 gtcaatcaac acgccattgc gttcaattcg agaaagtacg cccacaagtg gcatttcaat
    17761 ttcatcgaat aacttcacca attcaggctc ttgctgtagt ttttcccaca atacttggtg
    17821 gagtttcatc gtcacatcgg catcttctgc cgcatattca gccgcttttg ctatctcgat
    17881 tttatcgaag gtcagttgat ttttcccctt accagcaagc tcttcaaacg gaatcgtttt
    17941 atgccccaaa taacgggcgg ctaaatcgtc catattatga cgaccggtgc tgttttgggt
    18001 gtaagattca atcatcgtat caaaagcaac accttgtagc tcaatgccgt agttcgcaaa
    18061 aatggttaaa tcatatttaa tattctgccc gatcttttta atctccgggt taagcaaaat
    18121 cggcttgagt ttacttaaac aagcggtcaa atttagctga ttttttacca attcatcgga
    18181 agacgggctt tctgaacgct cctcatcacc gaacatatcg gcttgttgcg gttgggcttt
    18241 ttgcttatgc ccaagcggaa tatagcacgc ctcgccattt tccagtgcaa aggaaatacc
    18301 gaccaaatta gccgacattg cgtcaaggct gtcggtttcg gtatccaccg caatcaaacg
    18361 agctgcggtg agtttttcga tccaggctga taatttctgt tcagtatcaa cggtctcata
    18421 tttgctacga tcgatctcga ccacatttgc aacattttcc tcatttctga ccgcttgtgt
    18481 tacttggtaa ttattcggta ctttttcagc cgaagtttga gtgaccggat tggcattttg
    18541 attcacttcg ttcagccagc gtttaaattc ataatgctca aataattgag caagctcagc
    18601 acggtcttgc ggctgtgtta aaagttgatc gtgagtaaca tcaagttcca catcggtttt
    18661 gatggtagca agcaaatagg ataaatcggc attcgctttt tccgcttcca attttggggc
    18721 gaaatttttt gccccacgga aagaaagggt ggcaatttga tccaaattag cataaatctg
    18781 ttcaagtgaa ccaatgcctt gcaataaagc taatgcggtc ttttccccca caccttttac
    18841 gcccggaatg ttatcggcgg aatcgcccat taatgccaga aagtcgataa tcaactcagg
    18901 cggaatgcca tatttttcaa tcacgccttc acgatctaac aaagtattgt tcatcgtgtt
    18961 aatcaacata atgtgatcgt tcaccaattg tgccatatct ttatcgccgg tgctgatcaa
    19021 cacatctttg ccgtctttag cggcttgcac agcaagcgtg ccgatcacat catcagcttc
    19081 cacaccttca atcgaaatca gtggaatgcc taaggctttg ataatgcgat gcaaaggctc
    19141 aatttgaggg cgtaaatcat cgggcattgg tgggcggtgc gatttatatt gcttaaacaa
    19201 ctcatcacgg aaggttttcc ctttggcatc aaacaccaca gcaatatggc ttggattcac
    19261 ttgtgaaatc aggcttttta acatattcag cacgccatac attgcccctg tcggttcgcc
    19321 taagcgatta gtgagcggag ggaaagcgtg aaatgctcga tataaatagg aagaaccatc
    19381 caccaatacc agtggatttt ttgcgatagt tgccataaaa atctctttat aatcaaataa
    19441 atattggcgt tattatacaa gaatatttaa gaattgagtg tgtataaggc ttattttaaa
    19501 taaaacgttc cgaaaaaaca ttgaatttca ttaaaaaatt cctttattta tcaaataaat
    19561 gcgatttgtt tcggaacgtt tttttaatgc ttattttgca acatacggat tatcaatgga
    19621 aagcgttgct gtagacattg tgcctcctag tacaaaaaaa ccgcttggtt tccctaagcg
    19681 gttctaatct ataagaacta ttaaactaca atctgccccg tctcttttaa gtacttatag
    19741 atacgcccta acataatctg cgttggcgtt ttgccaatat cctcttttgt tagcggtgca
    19801 tcagtaatcg ctttggctga ttgagcatca atatatttgt actcacttgt gatgattaca
    19861 aagtttgtta cctcgccatc ggtatcatca cctgtgccaa caacatactt agcattgag
//
LOCUS       DANLRK010000023        19914 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.023, whole genome shotgun sequence.
ACCESSION   DANLRK010000023 DANLRK010000000
VERSION     DANLRK010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 19914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19914
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.023"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(87..725)
                     /locus_tag="PXA33_000628"
     CDS             complement(87..725)
                     /locus_tag="PXA33_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HDL5119988.1"
                     /translation="MRYTTEQIIEKLRQLKVVPVIALDKAEDILPLAKTLSENGLPVV
                     EITFRSEAAEEAIKLVQANYPEVLIAAGTVLTKEQVLTAKNAGADCIVTPGFNPTIVK
                     YCQVLGLPVTPGVNNPMAIEAALELGIQAVKFFPAEASGGVKMIKALLGPYGNLQIMP
                     TGGISPQNINDYLAIPNVVACGGSWFVEKSLIKEQKWEEIGKLTKEVVELVK"
     gene            complement(813..1760)
                     /locus_tag="PXA33_000629"
     CDS             complement(813..1760)
                     /locus_tag="PXA33_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDL5119989.1"
                     /translation="MKKLAILGECMIELNGEPFGEMHQTYGGDSLNTATYLARVSSPE
                     QIEVRYISALGTDKLSQGMLAAWQVDNINTDFVLRDEHRQPGLYLIQLDKQGERTFLY
                     WRNQSAARYLLQHPGYPKALSALNEVDMVYLSGISLAILPENDRTLLIAQLRELKTQG
                     VKIAFDSNYRPKLWESVAAAQAHYAELLQLVDLALVTFDDEQLLWGDVNEQATFDRLT
                     QYGIQTIVVKQGSLGATVLQNGERTFVPTVAVEKVVDTTSAGDSFNAGFLNGYLLGKD
                     VVTCCQQGNAVAGIVIQHKGAIIAKSATTHLKSQFEQSI"
     gene            complement(1777..2622)
                     /locus_tag="PXA33_000630"
     CDS             complement(1777..2622)
                     /locus_tag="PXA33_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL5119990.1"
                     /translation="MSIAQNHNFKDKVVVITGAGGVLCAYFAKEIAKTGAKVALLDIN
                     LEAAQNFASEIADEGYIAKAYQTNVLELESIKQARDEILKDFGTCDILINGAGGNNPK
                     ATTDNEFHDINLPENTKSFFELDKAGIEFVFNLNYLGTLLPTQVFAKDMLGKEGANII
                     NISSMNAFTPLTKIPAYSGAKSAISNFTQWLAVYFSKVGIRCNAIAPGFLVSNQNKAL
                     LFNADGTPTARANKILTNTPMGRFGQADELVGAILFLMDHHYSSFINGVVLPVDGGFS
                     AYSGV"
     gene            complement(2634..3905)
                     /locus_tag="PXA33_000631"
     CDS             complement(2634..3905)
                     /locus_tag="PXA33_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005668000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HDL5119991.1"
                     /translation="MLWLFLGSLFLFLFLGVPVALSMLLSASFMLWKMDMFDAQLITE
                     NFVMGTNNFPLMAIPFFMLTGEIMKHGGISERIINFAMSMVGHIKGGLGYVVIISGLI
                     FAGLSGSAVADTAALGAILIPMMVARKYDVARSTGLICAAGIISVVIPPSIPMIVYGI
                     TAGASITKLFMGGMVPGFLMVLGLWLVWKFLYRNEEQTVEPKKTGKERWAAFKSAFLP
                     LLLPIIIIVGLRGGIFTPTEAGVVAAIYAAIISIILKGLTLDKIQDVFLGTIKTTAMV
                     MFVAAAAMISAFAVTVAQIPMELVEFIKGITDSPTVLMFVIMIFLLLVGCVMDLIPAV
                     LIFVPVLLPLLRAYNIDIAYFGIMMVINLSIGLITPPVGTVLYVGSGIAKIGIARLTK
                     GIYPFLLVYAAILFLLILFPEIIIVPMNWLS"
     gene            complement(3908..4387)
                     /locus_tag="PXA33_000632"
     CDS             complement(3908..4387)
                     /locus_tag="PXA33_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HDL5119992.1"
                     /translation="MQISTALDRTLKLLAIIALSTMIILVFFNAVLRYFFDSGIAWSE
                     EFARICFVYMIFLGIILVAKERGHLTVDIVVSALPAKVQMYLLFFADILVLVALSFIT
                     YGAYQLMLLTYTQKMPATEISSAFLYFAAVISSISYFAIVFTNLWKHIRLAFIKEEK"
     gene            complement(4448..5431)
                     /locus_tag="PXA33_000633"
     CDS             complement(4448..5431)
                     /locus_tag="PXA33_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005693870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HDL5119993.1"
                     /translation="MKLKTFTKALLISSLMFTGSAFAKTIIKIGHYNSDVHPSNIALQ
                     EYFKKTIEEKTEGRYEIRIFPNNQLGGEDQIVNGLRNGTIEGGITGLLLQNVDPIFGV
                     WEWPYLFKDNAEAKKVLESPIAQEIGEKMEKYGIKLLAYGMNGFRVISSNKKVENFDG
                     FKGLRLRVPLNSLFVDWAKAMHINPQSMPLSEVFTALEQKVIDGQENPYMLIKDSGLY
                     EVQKYIIESNHIFSPGLLQLSLKTWNKMSKEDQTIFLEAAKLYQEKEWELAMKMEQDV
                     KDFFHKNGKEVIVPSEQFKADMLKASETLYQNFYQKYDWAKDVISKIHAAK"
     gene            complement(5460..6485)
                     /locus_tag="PXA33_000634"
     CDS             complement(5460..6485)
                     /locus_tag="PXA33_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HDL5119994.1"
                     /translation="MEKPLKSVCLVEPNKVGIQTVSYPVKGENDVLIKVEGMGICGSD
                     IGAFRGTNPLVTYPRILGHEIVGTVIESGKGMPNNINVGDRVIIEPYIYCGHCYPCSI
                     GRTNCCESLKVLGVHIDGAMQEVVRHPAHMIIKTPDMPIHELALAEPLTISLHAIHRT
                     KVKAGEHVAIIGAGAIGLMAALVAKAYGATPILIDILDKRLDYAKSIGIPHTINPTKE
                     NDLEAIKSITNGRMAEVVIEASGANVAVQNTLKYTSFAGRIALTGWPKTETPLPTNLI
                     TFKELNIYGARTSKGAFEEALELLASRRIEPKDIISKVITFDEIPHYIEELSANPDDY
                     LKIIAVF"
     gene            6649..7410
                     /locus_tag="PXA33_000635"
     CDS             6649..7410
                     /locus_tag="PXA33_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HDL5119995.1"
                     /translation="MQNSSRTYTKIGKILKEEIAQGKYRVGDKLPTERDISERFEVSR
                     TIVREAIVMLEVEKLIEVKKGSGVYVIRVPELFPEDFPEEKDNELRDVGPFELLQARQ
                     LLESSIAEFAAQQATKQDIQVLRNILESEKKLLLEKMDDYSADKEFHLALAHMTGNDV
                     LIQLQEQLWRYRFNSAMWAQLHSRILQHDHHHLWIEDHERILNAIQRKNPALARKAMW
                     QHLENVKTKLLELSDVEDPKFDGYLFNVNPVVVGI"
     gene            7440..8624
                     /gene="uxuA"
                     /locus_tag="PXA33_000636"
     CDS             7440..8624
                     /gene="uxuA"
                     /locus_tag="PXA33_000636"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012341261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HDL5119996.1"
                     /translation="MEQAWRWYGPNDPVSLDDVRQAGATGIVTALHHIPNGQVWSVEE
                     INKRKALLAEKGLVWSVVESVPVHEEIKTQTGNYQQWIDNYKQTLRNLASCGIDTVCY
                     NFMPVLDWTRTDLEYEMPDGSKALRFDQVAFAAFELYILKRPGAEATYSSAEQAEAKA
                     YFEQMSDADIAKLTKNIIAGLPGSEEGYTLEEFQAQLDRYQGLSAEKFRTHLAYFLNE
                     IVPVAQEVGIRMAIHPDDPPRPILGLPRIVSTIEDMQWFVETQPLAANGFTFCTGSYG
                     VLAENDLVKMAEQFADRIYFAHLRATQREENPQSFHEADHLAGDVDMFGVVKALLTEE
                     YRRKANGDHRLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLRGLAEFRGLELALKKVYF
                     ND"
     gene            8675..9544
                     /locus_tag="PXA33_000637"
     CDS             8675..9544
                     /locus_tag="PXA33_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDL5119997.1"
                     /translation="MFKKYFGEIILFLVTFIAAAGWFFSKHALVGLPPIGFMGGRFIL
                     ASLILLPFSYRELYRLDKVQVKYSVITGLTYGIYTIFWVLGLAFSTVFGEGAFLVSLA
                     MLIAPLLSWLIFKHTPPKIFWLALPIALSGLYFLSAEKGIFHFSLGSTIFLVSSVFGA
                     LYFVLNNQYAKQVSLFPLITIQLAIVGAVCSLYSYFMESWHFPILTEVWLWFAASVLI
                     ATNLRFLMQTLGQKYCHIGTSALIMLLEPVWTLLLSVMLLGEQLSATKWLGCSLILIA
                     LIIYRLPILRLKK"
     gene            9712..10026
                     /gene="rsfS"
                     /locus_tag="PXA33_000638"
     CDS             9712..10026
                     /gene="rsfS"
                     /locus_tag="PXA33_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HDL5119998.1"
                     /translation="MEQNLVEFLTKTLDDLKAQDILAIDVKGKSSITDTMILATGTSS
                     RHVASTAERLITEAKQAGFEVFGDEGKAVADWIVVDFGKAMVHLLQSDSREMYQLEKL
                     WA"
     gene            10103..10570
                     /gene="rlmH"
                     /locus_tag="PXA33_000639"
     CDS             10103..10570
                     /gene="rlmH"
                     /locus_tag="PXA33_000639"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010618862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HDL5119999.1"
                     /translation="MKIQLIAVGTKMPAWVTTGFEEYQRRFPKEMPFELVEIPAGKRG
                     KNADTKRILELEGKAMLAAAGKAKIVTLDIPGKAWTTEQLATQLEGWKNDGRDVALLI
                     GGPEGLSPECKAAAEQSWSLSPLTLPHPLVRVVVAESLYRAWSVTINHPYHRE"
     gene            10594..12543
                     /gene="mrdA"
                     /locus_tag="PXA33_000640"
     CDS             10594..12543
                     /gene="mrdA"
                     /locus_tag="PXA33_000640"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HDL5120000.1"
                     /translation="MFGKLAKFTASNQREKVRDGQAESNLYARRALVAFLGVLALSGV
                     LLTNLYHLQVVNYEDYQTRSNGNRIKLLPVPPARGLIYDRNGKVLAENLTYFGLFIVP
                     EKTENIEQTLTELKDIVGLTDEDIENYHKEKKRSSRYTPIMLKGNLNEAQMARFAVNQ
                     YRFPSLDVQAYYKRNYPYGELLTHIIGYVAKINENDKKKLQEAGKFGNYAGSHDMGKL
                     GIEKFYEDQLHGVTGFEEVEINNRGKVIRKLRDQAGVAGSSIRLSIDVELQQYVMSLI
                     AKHKGAVVVLDPRDSSILAMVSNPSYDNNLFVGGISGQDYKALLDDPTRPLYSRTTQG
                     TYPPASTIKPFMAVAGLEEGMITPSTTISDPGYWILPGTTQRYRDWKRSGHGATNVNK
                     AITESSDTFFYQLAYKMGIDKMSDWMKKFGFGVKTGIDIFEESAGVMPTREWKQKRYK
                     KPWVQGDTIPVGIGQGYWISTPLQLAKATAVLINNGRVNTPHLMLEVQSSNSVTPYQD
                     PLLYEDIKGVPDVYWNLAKQGMYNVNHAANGTARKVFAGAFYHSAGKTGTAQVFNLNG
                     KNYDKNAIKKELHDHAWFIGYAPYEQPKIVVALILENAGGGGSAAAPVARQIMDYALR
                     KGIGEQPDSQNSEQNKPLVTEEHHE"
     gene            12536..13663
                     /gene="rodA"
                     /locus_tag="PXA33_000641"
     CDS             12536..13663
                     /gene="rodA"
                     /locus_tag="PXA33_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HDL5120001.1"
                     /translation="MNSGFKKLFWKIFSLDLLLLSGLLAITGYGLLVLYSASGANERM
                     FTSRLIQVSLGLGLMLIMAMVPPRFYQKVSPYLYIFCLILLILVDLVGETSKGAQRWL
                     NLGFVRFQPSEIAKLAVPLMVATYLGKRPLPPSFKDTFIALAIIIVPTLLVAIQPDLG
                     TSILVCSAGIFVLFLAGLSWKLIGAGVVFLAGFIPIMWFFLMHDYQKTRVMTLIDPNK
                     DPLGAGYHIIQSKIAIGSGGINGKGWMEGTQSQLEFLPEPHTDFIFAVLSEEHGMIGV
                     LILLAIYLFIIARGLVIGTKSDSAFGRILSGGTALLFFVYVFVNIGMVSGILPVVGVP
                     LPLFSYGGTSYVTLMAAFGLMMSTYVHRKRVGSNNPYDKLK"
     gene            13755..14414
                     /locus_tag="PXA33_000642"
     CDS             13755..14414
                     /locus_tag="PXA33_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HDL5120002.1"
                     /translation="MRLGKIVTLLLAGFLTFSSLNSLAATKNTKAKSSILAKKQATSK
                     AIKKVGISKVKKKEVVAKATKKKTISTVTTKPIKSTKKLARKVVKKTVTAKKKLVKKQ
                     HKPLVKKHFQTGVASYYANYFNGRRTANGETFNNAKMTAAHRTLPFGTLIEVTNLRNG
                     RSVVVRVNDRGPYAHARVLDLSSAAAKKIGMHHSGTARVKIAIVDKSKLGSTNQFYVM
                     N"
     gene            14428..15606
                     /locus_tag="PXA33_000643"
     CDS             14428..15606
                     /locus_tag="PXA33_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HDL5120003.1"
                     /translation="MKKTLVKAMSVVGFAVSSIAYADLNTNFNLSAPQINAQTYILMD
                     YNSGEVLVSLNPDQRQYPASLTKMMTSYVVGEALKSGKVQNSDMVIVTENSWAQKFPG
                     SSLMFLDLNSKVSVGELMKGLIIVSGNDASVALAEHVSGSQAAFINEMNKYAQQFGLK
                     NTHFTTVHGLDDPEQYSSARDMAIIGSHIIRDQPEEYKIYAEKEFQYNIKKPQPNRNG
                     LLWDKTIQVDGMKTGHTDKAGYNLVASAINPNTRLISVVMGVPTYKGREVESKKLLQW
                     GFANFETIKSIPAAQSVAEQSVYYGEVNKVQLGSVQDSFVTIPKGRSADLKARYELEK
                     KNLEAPLAKGQVVGKMIYQLDGKDVASTNLQVLQDVPEAGIFGKAWDWIALTVKSLFD
                     "
     gene            15774..16073
                     /locus_tag="PXA33_000644"
     CDS             15774..16073
                     /locus_tag="PXA33_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HDL5120004.1"
                     /translation="MSQPKAINLQDLPQAKLKDLLEFPCHFTFKVVGANREDLVDDVV
                     VVTQKYAKGDYNPRQQKSSKGTYNSVSIDIVAENIEQVETLYTELAKINGVRMVL"
     gene            16135..16788
                     /gene="lipB"
                     /locus_tag="PXA33_000645"
     CDS             16135..16788
                     /gene="lipB"
                     /locus_tag="PXA33_000645"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HDL5120005.1"
                     /translation="MKLIIRQLGIQPYEEIWHQMQSFTDSRDGNTDDEIWLVQHPAVF
                     TQGSAGKPEHLLNPTNIPVVQSDRGGQITYHGEGQQIMYVLIDIKRLKSLGKEVSVRD
                     LVTALEQTVVQTLAEYGIESYPKADAPGVYVDGKKICSLGLRIRKGCSFHGLALNINM
                     DLTPFQNINPCGYAGLEMCQLSELVHRPDLRCEEVAPKLVAHFSTILGYNNQQIINN"
     gene            16803..17810
                     /gene="lipA"
                     /locus_tag="PXA33_000646"
     CDS             16803..17810
                     /gene="lipA"
                     /locus_tag="PXA33_000646"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HDL5120006.1"
                     /translation="MATSTAQTTATATVKKMSPFKMEKGVKYRDAAKTSVIQVRNIDP
                     DQELLKKPEWMKIKLPANSAKIDSIKNGMRRHGLHSVCEEASCPNLHECFNHGTATFM
                     IMGAICTRRCPFCDVAHGKPLPLDPDEPRKVAETVQDMKLKYVVITSVDRDDLPDRGA
                     AHFSATVREIKALNPHCKVEILVPDFRGRVEQAVAILKENPPDVFNHNLENVPRLYRE
                     VRPGADYKWSLELLKIFKQEFPHIPTKSGLMVGLGETNEEILEVMQDLRDHGVTMLTI
                     GQYLQPSRHHLKVERYVPPAEFDMFREEAEKMGFEHAACGPFVRSSYHADLQAKGELV
                     K"
     gene            17997..19100
                     /locus_tag="PXA33_000647"
     CDS             17997..19100
                     /locus_tag="PXA33_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL5120007.1"
                     /translation="MKKLLLNSVFAATLLAFGATANAEIKTITDVLGREVKVDVPAKR
                     VALTFYYPDYIAVTGVENFDKVVGISREFWEKFNPGSWALFAEKMPNLKNIADLGYVN
                     SGTFSTEKTIALKPDVLVLPEIQYQALASEISRIEQAGIPVVVDFNKQTIENHTKSVR
                     VFGQLTGTEERAEKIAKEYEEGIADIQSCIDNAKVSKPKIYAEFGNKGPKEHSFTFGK
                     NMWGAIAETVRGDNISAPFVENWGQINPEQVLVSKPDVIMISGTELGNDTNPEIMSMG
                     INIEEADAQKRLKGFIERTGWKELPAVKSGRVYGLYHTASRSISDLASAQFMAKALYP
                     ELFKDIDPEKTYLDFHNNYLPITPKGTFFMQLK"
     gene            complement(19191..>19914)
                     /locus_tag="PXA33_000648"
     CDS             complement(19191..>19914)
                     /locus_tag="PXA33_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL5120008.1"
                     /translation="VVADKINLHFEQGKIYTLLGPNGAGKSSLLKTLFGEIAHQGTID
                     FDGNKLTSSHLHSWRKEIGYMPQDTQVEASLSALEVILLGQLDSLNMYISDELLNEAV
                     DIMDKLGISHLAHQDIMRLSGGQRQMVMFAQVLLRKPHILLLDEPVSALDMYHQINLL
                     EYVNAYTRANNLITIMVLHDLSLAAQFSDSVMLLSEGKFKHKAELVICWNAILFRDSI
                     MSILRFYMMETARRLSARFANV"
BASE COUNT         5939 a         3836 c         4318 g         5821 t
ORIGIN      
        1 tatgcaatat ttgttatgct ttgtaaataa gaggaaagag atccattttg ggtagaaaat
       61 agctcattgc ttggaacttt aaatggttat ttcaccaatt caaccacttc cttcgtcaat
      121 ttcccaatct cctcccactt ctgttcctta atcagggatt tctccacaaa ccaagagccg
      181 ccgcaggcga ctacgttggg aatggcgagg tagtcgttga tgttttgtgg cgagatgccg
      241 ccggtcggca taatctgtaa gttgccgtaa gggccgagca gggctttaat cattttcacg
      301 ccgccactgg cttcggcagg gaaaaattta actgcttgta tgccaagttc gagggcggct
      361 tcaatcgcca tcgggttgtt cacacccggt gtaacaggca aacctaacac ttggcaatac
      421 ttaacgatag tcggattaaa acccggagtc acaatgcaat ccgccccagc gtttttggca
      481 gttaagactt gctctttggt cagcactgtg cctgctgcaa tcaatacttc cggatagttg
      541 gcttgcacta atttaattgc ctcttcagct gcttccgagc ggaaagtgat ttccaccacc
      601 ggcaaaccgt tttcggaaag ggttttcgcc agcggtaaaa tatcttctgc tttatccaaa
      661 gcaatcacgg gaacaacttt cagttgtctt aatttttcaa tgatttgttc ggtggtgtaa
      721 cgcatttcta gcctcattat gttgtattgc aagcggtcgc ttttgtaaaa aattttgcaa
      781 atttaaccgc ttgtcgttgt gctattgttg gtctaaatgg attgttcaaa ctgcgatttc
      841 aaatgagtag tggcggattt ggcaataatc gcccctttgt gctgaatgac gataccggca
      901 acggcatttc cttgttggca acaggtaacg acatctttac ccaataagta accgttaagg
      961 aagccggcat taaaggaatc gccggcggaa gtggtatcga ccaccttctc taccgcaaca
     1021 gttggcacaa aagtacgctc cccgttttgc aacaccgttg cccccagaga gccttgtttc
     1081 accacaatgg tctgaatgcc atattgagtg agacggtcaa aggtggcttg ctcattcaca
     1141 tcgccccaaa gcagttgctc gtcatcaaag gtcacgagag ctaaatcgac taattgcaac
     1201 agctcggcat agtgagcttg ggcagcagcc acactttccc acagtttcgg ccggtaattg
     1261 ctgtcaaagg cgattttcac cccttgtgtt ttgagctcac gcaactgtgc aatcagcaaa
     1321 gtgcggtcgt tttccggcaa gatggcaagg ctgataccgc tcaagtaaac catatctacc
     1381 tcgtttagtg ccgagagtgc tttgggatag ccggggtgtt gcaacaaata acgggcggca
     1441 gattgatttc gccaatagag gaacgttcgc tcgccctgtt tatccaactg aatcagatac
     1501 agccccggct ggcggtgctc gtcacgcaac acaaaatccg tgttgatatt atccacctgc
     1561 cacgccgcta acattccctg actgagctta tccgtgccga gagcggaaat ataacgcacc
     1621 tcaatctgtt caggcgaact cacccgagcc aaataggtag cggtgttgag cgaatcccca
     1681 ccataggttt ggtgcatctc cccaaacggc tccccgttta actcaatcat acattcaccg
     1741 agaatggcga gttttttcat aggaatacct tctaagttac acgccgctat aagcagagaa
     1801 tccgccatca acaggtaata caacgccatt gataaagctg gaataatggt gatccattaa
     1861 aaataggatt gcacctacca attcatcggc ttgtccgaaa cgtcccatag gggtattcgt
     1921 gaggatttta ttagctctag ctgttggcgt accatcggcg ttaaatagta gagctttatt
     1981 ttgattactc actaaaaatc ccggtgcaat agcattacag cggataccta cttttgagaa
     2041 ataaactgct aaccattgtg taaagttact gattgcagat tttgctccag aataggcagg
     2101 gattttagtt aatggagtaa aggcattcat actggatata ttaatgatat tagcgccctc
     2161 tttacccaac atatcttttg cgaaaacctg tgttggtaat agtgtgccta agtaatttaa
     2221 attaaataca aattcaatcc ctgctttatc taattcaaaa aaagattttg tattttccgg
     2281 taagttaata tcgtgaaatt cattatctgt cgttgctttt ggattattcc cacctgcacc
     2341 attaattaaa atatcacaag tgccaaaatc ttttagaatt tcatctcttg cctgttttat
     2401 gctttctaat tcaagtacat tagtttggta cgctttcgca atatagcctt catcggcaat
     2461 ttcagaagca aaattctgtg ctgcttctag attgatatcg agtagtgcaa cttttgcacc
     2521 tgtttttgca atttcttttg caaaataagc acatagcaca ccacctgctc cggtaataac
     2581 aacaacttta tctttaaaat tatgattttg ggcaatagac ataataagaa ctcctaagat
     2641 aaccaattca ttggtacgat aataatttca gggaaaagta ttaataagaa cagtattgca
     2701 gcatagacta gcaagaatgg atagatacct ttggttaatc tagcaatacc aatttttgct
     2761 ataccagaac caacatataa taccgttcca acaggaggcg taattaaacc gatagatagg
     2821 ttaattacca tcataatacc aaagtaagca atatcgatgt tatacgctct tagtaatggt
     2881 aatagtactg gtacaaaaat taatactgct gggattaagt ccattacaca gccgactaat
     2941 aataagaaaa tcataatgac gaacattagt acagtcgggc tatcggtaat acctttgata
     3001 aactcaacta actccatagg tatttgagca acagtaactg caaatgcaga tatcattgcc
     3061 gccgctgcaa caaacataac cattgcagtt gttttgatag tgcctaagaa aacatcttgg
     3121 attttatcta aggttaaccc ttttaagata atgctgatta ttgctgcata aattgctgct
     3181 acaactcccg cttctgttgg tgtaaagatg ccgcctctta aacctacgat aataataatt
     3241 ggaagtagca atggtaagaa tgctgattta aatgcagccc aacgttcttt acctgttttt
     3301 ttcggttcaa cggtttgttc ttcgtttcgg tataagaatt tccaaactaa ccataaacct
     3361 aaaaccatta aaaagcctgg aaccattcct cccataaata atttggtaat tgatgcaccg
     3421 gctgtaatac catagacaat cattggaatg cttggtggga taactacaga aataatacct
     3481 gctgcacaga ttaagcctgt tgaacgtgca acatcatatt ttcttgcaac catcatcgga
     3541 ataagaattg caccgagagc cgcggtatca gcaacagcgg agccggataa acctgcgaaa
     3601 attaagcctg aaataatcac tacatagccc aatccgcctt taatatggcc gaccatgctc
     3661 attgcaaaat tgataatacg ttcggaaata ccgccgtgtt tcataatttc accggtcagc
     3721 ataaaaaacg ggatagccat cagcggaaaa ttatttgtac ccattacgaa attctccgtg
     3781 attaattggg catcaaacat atccattttc catagcataa aactggcact aagtagcata
     3841 gagagagcaa cgggcactcc taaaaagaga aagaggaata gacttcctaa aaatagccat
     3901 aacatattta tttctcctct ttaatgaaag ctagccgaat atgtttccat aaatttgtga
     3961 atacaatagc gaaataggag atagatgaaa taactgctgc aaaatataga aacgcagatg
     4021 aaatttcagt tgctggcatt ttttgtgtgt atgtcaataa cattaactgg tatgcaccgt
     4081 aagtaataaa tgacagtgca actaagacta aaatatcagc aaagaacagt aagtacattt
     4141 gtacttttgc cggtagtgca gaaacaacaa tatcaacggt gaggtgtccc ctttcctttg
     4201 caacgaggat tattcctaga aagatcatat agacaaagca gattctggcg aattcctcag
     4261 accaagctat tccggagtca aaaaaatatc gtaaaaccgc attaaaaaag actaggataa
     4321 tcattgtaga tagagcaatt atcgcaagga gtttgagagt tctatctaat gctgtagaaa
     4381 tttgcataaa tttctccttc ttcatttaga tgggcgacct cttttatccg gtcgcctcgc
     4441 ttattgctta tttggctgcg tggatttttg aaataacatc tttagcccaa tcatatttct
     4501 gatagaaatt ttgataaagc gtttcggatg cttttaacat atcagcttta aattgttcac
     4561 tcggaacaat gacttctttg ccatttttgt ggaagaaatc ttttacatct tgttccatct
     4621 tcatcgctaa ttcccactct ttttcttggt agagttttgc tgcctctaag aagattgtct
     4681 gatcttcttt agacatttta ttccaagttt tcaaactaag ttgtagtaag ccaggtgaga
     4741 aaatatgatt agattcaata atatattttt gcacctcgta taaaccagaa tctttaatta
     4801 acatatatgg attttcttgc ccatcaatga ctttttgttc tagtgcggta aatacttcac
     4861 ttaaaggcat actttgaggg ttaatatgca ttgcttttgc ccagtccaca aacaacgaat
     4921 ttagtggaac ccttaatcgc aaacctttaa atccatcaaa attctcaact tttttattgg
     4981 aagagataac acggaagcca ttcataccat acgctagtaa tttaatgccg tacttctcca
     5041 ttttttcccc gatttcttga gcaataggag attctagaac tttcttagct tcggcattat
     5101 ctttaaatag atatggccac tcccatacgc caaaaattgg atcaacattt tgtagtaata
     5161 aacctgtaat gccaccttca atcgtgccat tacgcaaacc atttacaatt tgatcttctc
     5221 ctcccaactg gttatttggg aaaatacgga tttcgtagcg tccttctgtt ttttcttcaa
     5281 tggttttttt gaagtattcc tgcaatgcga tatttgatgg atgaacatct gaattatagt
     5341 gaccaatctt aatgatagtc ttcgcaaatg ccgaaccggt gaacatcagg ctagaaatta
     5401 atagagcttt agtgaaagtt ttgagtttca tagttattac ctcgtaggtg agttagtatt
     5461 taaaatacag cgatgatttt taagtaatca tcaggattag ctgagagttc ttcgatatag
     5521 tgaggtattt catcaaatgt aattacctta ctaatgatat cttttggttc aattcttctt
     5581 gatgccagaa gctctaatgc ttcttcaaat gcccccttgc ttgttcttgc cccataaata
     5641 ttaagttctt tgaaggtgat taaattagtt ggtaatggtg tttctgtttt cggccatccg
     5701 gttaaagcaa tacgtccagc aaaagaggtg tatttaagcg tattttgtac tgcgacattt
     5761 gctcctgacg cttcaataac cacctctgcc attcttccat tggtaattga tttaatagct
     5821 tcaagatcgt tttcttttgt tggattaatt gtgtgtggaa taccaataga ttttgcgtaa
     5881 tccaatcgct tatctaaaat gtcaattaaa atcggcgtcg ctccataagc tttggcaacc
     5941 aatgcagcca ttagcccaat tgcccctgct ccaatgatag caacatgttc accagctttg
     6001 acttttgttc tatgaattgc gtgtaaggaa atagttaagg gttctgctaa cgctaattcg
     6061 tgtattggca tatcaggtgt tttgattatc atatgggctg gatgtctaac aacctcttgc
     6121 attgccccat caatatgtac ccccaatact tttagagatt cgcaacaatt tgttctacca
     6181 atggagcagg gatagcaatg tccacaatag atgtaaggtt caatgatgac acgatctcct
     6241 acattaatat tatttggcat acctttacca gactcaatta ctgtgccaac gatttcatgc
     6301 cctaaaattc taggataagt aacaagtgga ttagtgcctc taaatgctcc aatatcagag
     6361 ccacatatcc ccataccttc tactttgatt agcacatcat tttcaccttt aacaggataa
     6421 gaaacggttt gtataccaac tttattaggt tctacaagac atactgattt gagtggtttt
     6481 tccatgattt tgtccttttt tgataaagtt aatcgacatt ttgtagtgtc aatggtattt
     6541 tatgcacaga tactaacacc aaaaaacaag tttggcattc caatttttgt gattttgtga
     6601 acctgatcac aaaaatgaaa ttttggaatt ccaatttcgt gaggatttat gcaaaatagt
     6661 tccagaactt acactaaaat aggaaaaatt ctcaaagagg aaattgctca aggaaaatac
     6721 agagtaggag ataagttacc tactgagaga gatatttctg aaagatttga agtgagtaga
     6781 actatcgtaa gggaggcgat agtgatgcta gaagtagaaa agcttattga ggtaaaaaaa
     6841 ggatcgggcg tgtatgtcat tagagtcccg gagctttttc cagaagattt tccagaagag
     6901 aaagataatg agctaaggga tgttgggcca tttgaactac ttcaagcacg acaattatta
     6961 gaaagcagta ttgctgagtt tgcagcacag caggctacca aacaagatat acaagtactt
     7021 cgtaacatat tagaaagtga gaagaaactt ttgttagaga agatggatga ctacagtgca
     7081 gataaggaat ttcatcttgc attagctcat atgacgggta acgatgttct gattcaatta
     7141 caagaacagc tttggcgata ccgctttaat agtgcgatgt gggctcagct acactccaga
     7201 attttacagc atgatcatca ccacttgtgg atagaggatc atgagcgaat tttaaatgca
     7261 atacagagaa aaaatcctgc attagctcgt aaagcaatgt ggcaacatct agaaaatgtt
     7321 aaaactaaat tgcttgaatt gtctgatgta gaggatccta aatttgatgg atatctattc
     7381 aatgtaaacc ccgttgtagt aggtatctaa aacctaaact acacaatgag gagtttacta
     7441 tggaacaagc atggcgttgg tatggaccaa acgatccggt atctttagat gatgtaagac
     7501 aagcaggggc aacggggatt gtgacagcgt tgcaccatat tcccaacggg caggtgtggt
     7561 cggttgagga aattaataaa cgtaaggcat tattagctga aaaagggctg gtttggtcgg
     7621 tggtagaaag tgtgcctgtt cacgaagaaa tcaaaactca aacaggcaac tatcagcaat
     7681 ggattgataa ctataagcaa accttgcgta atttggcaag ctgtgggatt gatacggtgt
     7741 gctataactt tatgccggtg ctagattgga ctcgtaccga tctggagtat gaaatgcctg
     7801 atggctcaaa agcattgcgt tttgaccaag tggcgtttgc ggcgtttgag ttatatatct
     7861 taaaacgccc gggagcggaa gccacttact catcagcaga acaagccgaa gccaaagcct
     7921 attttgagca gatgagtgac gctgatattg caaaattaac caaaaatatc attgccggtt
     7981 tgccggggtc tgaggaaggc tatacgctcg aagaattcca agcacagctt gaccgctatc
     8041 aagggctctc ggctgaaaaa ttccgtaccc atttagccta tttcttaaat gagattgtgc
     8101 cggttgcaca agaggtgggc attcgtatgg cgattcaccc tgacgatccg cctcgcccga
     8161 ttttaggctt gccacgcatt gtttcaacca tagaagatat gcaatggttt gtggaaactc
     8221 aaccgcttgc agccaatggc ttcacgttct gtaccggttc atacggcgta ttagccgaga
     8281 atgatttggt caaaatggca gagcagtttg cagatcgcat ttattttgct cacttacgag
     8341 ctacccaacg tgaagaaaat ccacaaagtt tccacgaagc ggatcatctt gccggtgatg
     8401 tcgatatgtt tggcgtggtg aaagcattac ttaccgaaga gtatcgccgc aaggcaaatg
     8461 gcgatcaccg attaattcca atgcgtccgg atcacggcca tcaaatgctc gatgatctga
     8521 agaaaaaaac caacccaggc tattctgcga ttggtcgttt aagaggatta gcggagttcc
     8581 gtggattgga gttggcactt aagaaagtct attttaacga ttagccttat ttattataat
     8641 gccctctact gtagagggca ttttatagtt tgatatgttc aaaaaatatt tcggcgaaat
     8701 aatccttttt ctcgttacct ttattgctgc agcggggtgg tttttctcta aacacgcttt
     8761 agttggatta ccgcctattg gctttatggg agggcgtttt atattagcct ctttgattct
     8821 tctgcctttt tcttaccgag agctgtatcg tttagataag gttcaagtta aatattcggt
     8881 gattaccggt cttacttacg gaatttatac tattttttgg gtattggggc tggcttttag
     8941 tacagtgttt ggtgaagggg cttttttggt gagccttgcg atgctgattg ctcctttgct
     9001 ctcttggttg atattcaaac atacgccacc gaagattttt tggttagcct tacccattgc
     9061 attaagtggg ctttactttc tttctgcgga gaaaggcatc tttcattttt ccttaggcag
     9121 tacgattttt ctggtgtcat cagtgtttgg ggcgttatat tttgtgttaa ataatcaata
     9181 cgccaaacaa gtctcattgt ttccattaat tactatccaa ttggcgatag tcggtgcggt
     9241 ctgtagcttg tattcctatt ttatggaaag ttggcatttc ccgattctca ccgaagtgtg
     9301 gctctggttt gccgcaagtg tattgattgc gacaaattta cgttttttaa tgcaaacact
     9361 ggggcaaaaa tattgccata ttggtacttc tgcactcatt atgttacttg agccggtttg
     9421 gacattgtta ttgagcgtga tgttattggg ggaacaactt agtgcgacaa aatggctagg
     9481 ttgcagcttg attttgatag cattgattat ttatcgactc cctattctac gactcaaaaa
     9541 ataggtaaaa caaaagcaaa tatgccatag tattggaagc agttaggcat tttagagtaa
     9601 gtaacaagcg gtcatttttg caaaaaatct tgcaaaaatg accgctttct ttcttttatc
     9661 cgttcaatct atgttaaaat tctacatttt tataagatga gagagagaaa cgtggaacag
     9721 aatttagtcg aatttttaac caaaacctta gatgatttaa aggcacaaga tattttagcc
     9781 attgatgtga aagggaaatc tagcattact gatacgatga ttttagccac cggtacatcg
     9841 agccgtcacg ttgcttcaac ggcagagcgt ttaattacgg aagcaaaaca ggctgggttc
     9901 gaggtatttg gagatgaagg taaagcagtt gccgattgga tcgtggtcga ttttgggaag
     9961 gcaatggttc acttgttgca atccgatagc cgtgagatgt accaattaga gaaactttgg
    10021 gcgtagcagt tcaataagga acgacaagcg gtgatttttg caacattttt tgcaaattaa
    10081 accgcttgta agtgaaagag agatgaaaat tcaattaatt gcggttggca ctaaaatgcc
    10141 ggcgtgggta acgacagggt ttgaagagta tcaacgccgt tttccgaaag agatgccgtt
    10201 tgagttggtg gaaattccgg caggaaaacg gggcaagaat gctgatacta aacgcattct
    10261 tgagcttgaa ggcaaagcaa tgttggcggc agcgggcaag gcaaaaatcg tgactttgga
    10321 tattccgggc aaagcgtgga caaccgagca gttagccact cagttagagg gctggaaaaa
    10381 cgatgggcga gatgtagcgt tattgattgg cggcccggaa ggattatcac cggaatgcaa
    10441 agccgctgcc gagcagagtt ggtcgttgtc gcctttaacc ttacctcacc cactggtgcg
    10501 ggtggtggtg gcggaaagtt tgtatcgggc gtggtcggtt accattaatc acccttatca
    10561 tcgagagtag tgagtcattt tagatagtgt tatatgtttg gtaaattagc aaaattcaca
    10621 gcttcaaacc agcgagaaaa ggtgcgagac gggcaggcag aaagcaatct gtatgcccgc
    10681 cgtgccttag ttgcgtttct gggggtgttg gcgttaagtg gtgttttatt aaccaacttg
    10741 tatcacttgc aagtggtgaa ttatgaggac taccaaaccc gttcaaacgg taaccgcatt
    10801 aaattgttgc ctgtgccacc ggctcgaggc ttaatttatg accgtaacgg taaagtattg
    10861 gcggaaaatt taacctattt cgggctgttt attgtgcctg aaaagacgga aaacattgag
    10921 cagactttaa ccgagttgaa ggatattgtt gggctgacgg acgaagatat cgagaattat
    10981 cataaagaga aaaaacgcag ttctcgctat accccaatta tgctcaaagg caatttgaat
    11041 gaggctcaga tggctcgttt tgcggtgaac caataccgtt ttccaagttt agatgtgcag
    11101 gcgtattata agcggaatta tccttacggc gagcttttaa cccatattat cggttatgtg
    11161 gcgaaaatta acgaaaacga taagaaaaag ctgcaagaag ccgggaaatt cggcaattat
    11221 gccggttcgc acgatatggg gaaattgggg attgaaaaat tctatgaaga tcagctccac
    11281 ggtgtcaccg gttttgaaga ggtagaaatt aataaccgag ggaaagtaat ccgtaaattg
    11341 cgtgaccaag caggggttgc cggtagcagt attcgcttga gtattgatgt ggaattacag
    11401 caatatgtga tgtcgttaat tgctaagcat aaaggggcgg tggtggtgct tgaccctcgt
    11461 gatagcagta ttttagcgat ggtctcaaac ccaagttatg acaataattt atttgtgggc
    11521 ggtatttcag ggcaggatta taaagcatta ttggacgatc caacccgacc actttatagc
    11581 cgtactacac aaggcactta tccaccggct tcaactatta agccatttat ggcggtggca
    11641 ggtttggaag agggaatgat taccccttct accaccattt cagaccccgg ctattggatt
    11701 ttgcccggca ctacacaacg ttatcgagat tggaaacgta gcggacacgg tgcaactaat
    11761 gtgaataaag caattaccga atcgtctgat acctttttct atcaattagc ctataaaatg
    11821 gggattgata aaatgtcgga ttggatgaaa aaattcggtt tcggagtgaa aaccggtatc
    11881 gatatttttg aggaatccgc aggggtaatg ccaacccgtg aatggaaaca aaaacgctat
    11941 aaaaaacctt gggtgcaggg cgatactatt ccggttggga ttgggcaggg ctactggatt
    12001 tcgaccccgt tacagttggc gaaagccacg gcggtgttaa tcaacaacgg gcgggtgaat
    12061 acaccgcatt taatgcttga agtgcaaagc agtaattcgg ttacgccgta tcaagaccca
    12121 ttgttgtatg aagatattaa aggcgtgccg gatgtctatt ggaatttagc aaaacaggga
    12181 atgtataacg taaaccacgc cgcaaacggt acggcacgaa aagtgtttgc cggtgcattt
    12241 tatcattcag cagggaaaac cggtacggct caggtgttta acttaaacgg taaaaattac
    12301 gataaaaatg cgattaaaaa agagttgcac gaccacgctt ggtttatcgg ctatgctcct
    12361 tatgaacaac ctaaaatagt ggtggcatta attttagaaa atgcaggcgg tggtggtagt
    12421 gccgcggctc ctgttgctcg tcagattatg gattatgctt tgagaaaagg cattggtgaa
    12481 cagccggatt cgcaaaattc tgagcaaaat aaaccgcttg tcacagagga acatcatgaa
    12541 tagtggcttt aaaaaacttt tctggaaaat tttttcactc gatctccttt tattatcagg
    12601 attattagct attaccggtt atggtttatt agtactatat agcgcaagcg gtgcaaatga
    12661 gcggatgttt accagtagac tgattcaagt ttcgctaggg cttgggctaa tgttgataat
    12721 ggcaatggta cctcctcgct tttatcaaaa agtttcgcct tatctgtata ttttctgtct
    12781 gatattattg attttggtgg atttggtcgg cgaaaccagt aaaggagcgc aacgttggtt
    12841 gaatttagga tttgttcgct ttcagccttc tgagattgcc aagctcgctg tgccactgat
    12901 ggtagccact tatttaggta agcggccgct tcctccgagt tttaaagata cttttatcgc
    12961 cttagccatt attattgtgc caaccttatt ggtggcaatt cagcccgatt tgggaacatc
    13021 tattttagtg tgttctgccg gtatttttgt tttatttttg gcagggctaa gttggaaatt
    13081 aattggcgca ggtgtggtgt ttttagccgg ttttatcccg ataatgtggt ttttcttaat
    13141 gcacgattat caaaaaaccc gggtgatgac cttaattgat cctaataaag acccgttggg
    13201 tgccggctat catattattc aatccaaaat tgcgataggc tctggtggca ttaatggaaa
    13261 aggctggatg gaaggaacgc aatcccagct tgaattttta cctgaaccgc ataccgattt
    13321 tatttttgca gtgttaagcg aagagcatgg tatgataggt gtgctgattt tattagcgat
    13381 ttatttgttt attattgccc gaggtttagt gattggcaca aaatcggatt ctgctttcgg
    13441 gcgtatatta tcaggtggta cagcccttct attttttgtt tacgtttttg taaatattgg
    13501 tatggtgagt ggtattttac ccgtagtcgg tgtaccatta ccgcttttca gttacggcgg
    13561 aacctcctat gtgacgctga tggcggcatt tggtttgatg atgtcaacct atgttcaccg
    13621 taagcgtgta ggtagtaata atccttatga taaactgaaa taaattaaac ttttgctttt
    13681 ttgcggtatc aaaaagcagt gaaacgatca tcgttacaaa caataacgac atccacatag
    13741 aggaaaaaac ttatatgaga ttaggtaaaa ttgtaaccct gttgttagct ggatttctga
    13801 catttagttc attaaattct ttggctgcga caaagaatac aaaagctaaa tcgagtattt
    13861 tagccaagaa gcaagcaact tctaaagcaa taaaaaaagt aggtatttct aaagttaaga
    13921 aaaaagaagt agtggctaaa gctacaaaaa agaaaacaat ttctactgtt acaactaagc
    13981 cgattaaatc tacaaaaaag ctggctcgca aggttgtcaa gaaaacggta accgctaaga
    14041 aaaaattggt aaaaaagcag cataagccac tggtcaaaaa acatttccag actggtgttg
    14101 ccagctatta tgcaaattac tttaatggtc gccgaaccgc taatggggag actttcaata
    14161 acgcaaaaat gacggctgca catcgaactt taccatttgg tactttaatt gaagtgacga
    14221 atttacgaaa tggtcgttct gtggtggtgc gtgtaaatga tcgcggacct tatgctcacg
    14281 cacgggtctt ggatttatca tcagccgcag cgaaaaaaat cggtatgcac cattcgggga
    14341 cggcaagagt gaaaattgcg attgttgata agtcaaaatt gggatcgact aatcagtttt
    14401 atgtaatgaa ttaatttaag agggaatatg aaaaaaacat tagtgaaagc gatgagcgtg
    14461 gtaggttttg cggtttcaag cattgcctat gcggatttaa acactaactt caatttatcg
    14521 gcaccccaga tcaacgcaca aacctatatt ttgatggatt acaactccgg tgaagtttta
    14581 gtgagcctaa atccggatca acgtcaatat cctgcatcat tgaccaaaat gatgactagc
    14641 tacgtagttg gtgaagccct aaaatcggga aaagtgcaaa acagtgatat ggtgatagtc
    14701 accgaaaatt cgtgggcgca aaaattcccc ggctcttcat taatgttttt agatttaaac
    14761 agcaaagttt cagtaggtga gttgatgaaa gggctgatta tcgtctcggg taatgatgcc
    14821 tctgtggcgc ttgctgaaca tgtttcaggc tcacaagcgg cgttcattaa tgaaatgaac
    14881 aaatatgccc aacagtttgg cttaaagaat actcacttta ccactgttca cggtttagat
    14941 gaccctgagc aatattcttc tgctcgtgat atggcgatta ttggttcgca tattattcgt
    15001 gatcagccgg aagagtataa aatttatgcg gaaaaagagt tccaatataa catcaaaaaa
    15061 ccacaaccaa accgcaacgg attattgtgg gataaaacca ttcaagttga tggtatgaaa
    15121 acagggcata ctgataaagc cggctataac ttagtcgcat cagcaattaa cccgaataca
    15181 cgtttaattt ctgtagtgat gggcgtgcca acttataaag ggcgtgaagt ggaaagtaag
    15241 aaattactgc aatggggctt tgcgaacttt gaaaccatta aatctattcc ggcagctcaa
    15301 tcggtggcag agcaaagtgt ctattacggt gaagtcaata aagtacaatt aggctcggtg
    15361 caagatagct ttgtgactat cccgaaaggt cgctcggcag atttaaaagc ccgttatgag
    15421 ttagagaaga aaaacctcga agctccttta gcaaaagggc aggtagtggg taaaatgatc
    15481 taccaacttg acggcaaaga tgttgcaagc actaacttac aagtcttaca agatgtaccg
    15541 gaagccggta ttttcggcaa agcgtgggat tggattgccc ttactgtgaa gagcttgttc
    15601 gattaatctt gttatttgcc tttttatccc catctaggga gcaatgcaag cggtgtgatt
    15661 ttgcaaaaaa tttgcaaaat ttcaccgctt accgttttag atatgaatgt ataaaaagtg
    15721 tagtggcgaa aaatttttcg cccctcgaag aaagaataga agaaggatca aaaatgtctc
    15781 aacccaaagc gataaactta caagatttac ctcaagccaa attaaaagat ctgctagagt
    15841 ttccgtgcca tttcacgttc aaagtggtgg gagcaaaccg agaagatttg gttgatgatg
    15901 tggtagtggt cacgcaaaaa tatgccaaag gcgattacaa tccacgtcag caaaaaagct
    15961 caaaaggtac ctataattcc gtttcgattg atattgtggc ggaaaatatt gagcaagtag
    16021 aaactctcta cacggaactt gccaaaatta atggcgtaag aatggtatta taatgcgagg
    16081 ggcgaatgac attcgccctc aatttttcaa taaagcgatg ttggaaaata gaaaatgaaa
    16141 ttaataattc gccaactagg cattcagcct tatgaagaaa tttggcacca aatgcaatct
    16201 ttcacagata gccgtgatgg gaataccgat gatgagatct ggctggttca gcacccggct
    16261 gtgtttacgc aaggatcggc aggcaaaccg gagcatttgt taaatccaac caatattccg
    16321 gtggtgcaaa gcgaccgtgg cggtcaaatt acttaccacg gcgaggggca gcaaattatg
    16381 tatgtgctga ttgatatcaa acggctgaaa tccttgggta aagaagtgtc ggttcgagat
    16441 ttagtgactg ccctggagca aaccgtggta caaacgttgg cagaatatgg catagaaagt
    16501 tatccaaaag ccgatgcacc cggggtgtat gtggacggca agaaaatctg ctcacttggt
    16561 ttacgaattc gaaaaggttg ctctttccac ggtttggcat taaatatcaa tatggactta
    16621 acgccttttc aaaacatcaa tccgtgcggt tatgcagggc tggaaatgtg ccaattaagt
    16681 gaactggttc atcgccctga tttgcgttgc gaagaggttg ctccaaaatt agtggctcat
    16741 ttcagcacaa ttttaggcta taataaccaa caaattatta acaattagga taaaacacag
    16801 taatggctac atcgacagct caaacgacgg caactgctac agtgaaaaaa atgagccctt
    16861 tcaaaatgga aaaaggggtg aaatatcgtg atgccgccaa aacctctgtg attcaggtgc
    16921 gtaatatcga cccggatcaa gaactcttaa aaaaaccgga gtggatgaaa atcaaactgc
    16981 cggcaaattc ggcaaaaatt gatagcatta aaaacggtat gcgtcgccac ggcttgcact
    17041 cggtgtgcga ggaagcctct tgcccgaatt tacacgaatg ttttaaccac ggtactgcaa
    17101 cctttatgat tatgggggca atctgtaccc gtcgttgccc gttctgtgat gtggcacacg
    17161 gcaaaccgct tccgcttgac ccggacgaac ctcgcaaagt ggcagaaacg gtgcaagata
    17221 tgaaactcaa atatgtggtg attacctcag tggatcgtga tgatttgccc gaccgtggtg
    17281 cggctcattt ctctgcgacc gtgcgtgaaa tcaaagcctt aaatccgcat tgtaaggttg
    17341 aaattttagt gcctgatttc cgtggacgtg tggagcaagc ggtggcaatt ctcaaagaaa
    17401 atccacctga tgtctttaac cacaacctag aaaatgtgcc acgtctctat cgtgaagttc
    17461 gtccgggagc ggattacaaa tggtcgttgg aattattgaa aatcttcaag caagagttcc
    17521 cgcatattcc gaccaaatcc ggcttaatgg taggcttagg tgaaaccaac gaagaaatct
    17581 tggaagtaat gcaagattta cgagaccacg gcgtgacgat gctcaccatc gggcaatact
    17641 tacaaccaag ccgccaccac ttaaaagtgg aacgttatgt gccaccggca gagtttgata
    17701 tgttccgtga ggaagctgaa aaaatgggct ttgaacacgc agcctgcgga ccgttcgtgc
    17761 gttcttctta ccacgcggat ttacaagcga aaggcgaatt ggtgaaataa aatcgataag
    17821 ggcgaatatt atttgccctt tgtttttgtg gtttgcaaag ttttagcaaa attaaaccgc
    17881 ttgtaatctc ttctaattta ctcttctata gataatttcg ttgttgcgaa ttattttcat
    17941 tgactatttg ttatagaaag ttacaattca actttccatt tctttttgga gttccaatga
    18001 aaaaattact attaaacagc gtatttgctg ccacattgct tgcttttggt gcaactgcca
    18061 atgccgaaat taaaaccatc actgatgtat tagggcgtga ggtcaaagtt gatgtgcctg
    18121 ctaaacgtgt tgcactgact ttctattacc ctgactatat tgcggtaacc ggtgttgaaa
    18181 atttcgataa ggtagtaggg atttcacgag agttttggga aaagttcaac ccaggcagtt
    18241 gggcattatt tgcagaaaaa atgccaaatc taaaaaacat tgccgatctt ggctatgtga
    18301 atagtggtac tttttcaaca gaaaaaacca tcgcattaaa accagatgtg ttagtgctac
    18361 ctgaaattca atatcaagcg ttagccagtg aaatttcacg cattgagcaa gctggcattc
    18421 ctgttgtagt agatttcaac aagcaaacta ttgaaaacca tacaaaaagc gtgagagttt
    18481 tcggtcagct tacaggcact gaagagagag cggagaaaat tgccaaagag tatgaagagg
    18541 gcattgctga tattcagagc tgtattgata atgcaaaagt aagtaagccg aaaatctatg
    18601 ctgaatttgg taataaaggc ccgaaagagc atagttttac ctttggtaaa aatatgtggg
    18661 gagcgattgc cgaaacggtg cgaggcgata acattagtgc cccgtttgta gaaaactggg
    18721 ggcagattaa ccctgagcag gtgttagttt ctaaacctga tgtgattatg atttcaggta
    18781 cggaactagg caacgacact aaccctgaga ttatgtcgat ggggattaat attgaagaag
    18841 ccgatgctca aaaacgctta aaaggcttta ttgagcgtac aggttggaaa gagttacctg
    18901 cggtaaaatc agggcgtgtt tatggtttat atcatacagc ttctcgctca atttcagatt
    18961 tagcgagtgc tcaatttatg gcaaaagcac tttatcctga attgtttaaa gatatcgatc
    19021 ctgaaaaaac gtatttggat ttccacaaca actacttacc aattacgcca aaaggcacat
    19081 tctttatgca actgaaataa aaataacaag cggtcatttt gcagatttat tttgcaaaat
    19141 gaccgcttgt attcgctttt actatttgcg gttcaaacag caatcaatat ttaaacattt
    19201 gcgaagcggg cggataatcg gcgagccgtt tccatcatat aaaatctcaa tattgacatt
    19261 atagagtctc tgaataatat cgcattccaa catatcacaa gctctgcctt gtgcttgaat
    19321 ttcccctcgc ttaatagcat cacactatcc gaaaattgag ccgcaaggct taaatcgtgc
    19381 agcaccataa ttgtaattag gttattcgct cgagtgtagg cattcacata ttcaagtaag
    19441 ttgatttgat gatacatatc caacgcactg acaggctcat ctagcagcag aatgtgtggt
    19501 ttacgtagca atacttgtgc gaacattacc atttgccgtt gcccgccact tagccgcata
    19561 atatcttgat gagctagatg tgaaataccg agtttatcca taatatcaac ggcttcattg
    19621 agtaattcat cgctaatata catattcagg ctatcaagtt gccctaataa aataacctct
    19681 aaggcactga gtgaggcctc tacctgtgtg tcttgtggca tatagccaat ttctttacgc
    19741 caactatgca agtggcttga ggttaattta ttaccgtcaa aatcaattgt gccttgatga
    19801 gcaatttccc caaatagagt tttaaggaga gaagatttac ccgcaccatt tgggccaagt
    19861 aaagtataga tcttgccttg ttcaaaatgt agattgattt tatcagccac cact
//
LOCUS       DANLRK010000024        19599 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.024, whole genome shotgun sequence.
ACCESSION   DANLRK010000024 DANLRK010000000
VERSION     DANLRK010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 19599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19599
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.024"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            72..2681
                     /gene="adhE"
                     /locus_tag="PXA33_000649"
                     /gene_synonym="adhC"
     CDS             72..2681
                     /gene="adhE"
                     /locus_tag="PXA33_000649"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HDL5120009.1"
                     /translation="MAKNTQPFDAQAEVNELVEKGLKALDEFRKLNQEQVDFIVAKAS
                     VAALDKHGILAMHAYEETGRGVFEDKATKNLFACEYVVNNMRHLKTAGVISEDDVTGI
                     TEIADPVGVVCGITPTTNPTSTTIFKALIALKTRNPIVFAFHPSAQQSSAHAAQIVRD
                     AAVAAGAPENCIQWITQPSMEGTSALMKHPGIATILATGGNAMVEAAYSCGKPALGVG
                     AGNVPAYVEKTAKLKQAVYDIVMSKSFDNGMICASEQAAIVDKEIYAEFIKEMQSYGV
                     YLVNKKEKALLEKYIFGIDKAKDENCSGAKLNSAVVGKPAAWIAEQAGFKVPEKTNIL
                     LAECAFVGEAEPLTREKLSPVLALLKSDSTEHGLELSEAMVNFHGLGHSAAIHTENKA
                     LAKTFGERVKAIRVIWNSPSTFGGIGDVYNSFLPSLTLGCGSYGKNSVGNNVSAVNLI
                     NIKRVGRRRNNMQWFKVPSKIYFERDSIQYLKSMHDVHKVMIVTDRSMVDLGFVDRIT
                     EQLRQRRNQVMIQFFTDVEPNPSLQTVQRGTELMRSFQPDTIIALGGGSPMDAAKIMW
                     LFYEQPEVDFRDLVQKFMDIRKRAFKFPQLGRKAKFVGIPTTSGTGSEVTPFAVITDG
                     DIKYPLADYSLTPTIAIVDPALVMSVPSHVAADTGLDVLTHATEAYTSILANDYTDGL
                     ALQAIKLVFENLEKSVKEFDETAREKMHNASTMAGMAFANAFLGICHSMAHKIGGKFH
                     TIHGRTNAILLPHVIRYNGTRPTKVATWPKYTNYVADVRFQDIARMLGLPAETPEQAV
                     EAYAQAVHDLAVRCGVKMSLREQGVDEQAFLNARRELALHAFEDQCTPANPRLAMIED
                     MEAIMTKAYYGKE"
     gene            2828..3724
                     /locus_tag="PXA33_000650"
     CDS             2828..3724
                     /locus_tag="PXA33_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005612495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HDL5120010.1"
                     /translation="MQITILDGGISRELMRRNAPFHQPEWSAAALYEGPHFVQAVHED
                     FIAHGAEVITTDSYAVVPFHIGEKRFAADGKMLADLAGRLAKQAVKNSGNIATQIAGS
                     LPPMFGSYRADLIQMERFAEVAQPLIDGLSPYVDIWLCETQSAIIEPISIKALLPKDN
                     RPLWVSFTLTDDEPTTEPQLRSGESVKQAVEEMVKLGVQAILFNCCQPEVIGEALSVT
                     QKTLAELKATHIRTGAYANAFAPQPKDATANDGLDEVRQDLTLEAYLGWAKKWTEQGA
                     TIIGGCCGIGVEYIETLAKNLK"
     gene            3734..5143
                     /locus_tag="PXA33_000651"
     CDS             3734..5143
                     /locus_tag="PXA33_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011199731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL5120011.1"
                     /translation="MSNKKIGLISLTALVLSSMIGSGIFSLPQNMAAVAGAEAITIGW
                     LITGVGIIFLGLSFFFISRLRPDLDGGIYTYAREGFGELMGFMSAWGYWLCATIGIVG
                     YLTVAFEGLGVFTDNPNTVIFGQGNTLASFIGSSIIVWLVHALIAGGIKEAAFVNLIA
                     TFVKVAPLILFIGLGIWYFDVDLFNSDVKATALNNSVSDQVKSTMLITLWVFTGVEGA
                     SVLSAHAKKRSDVGLATVLGIIIALALYVAITILSLGILPRETIANMANPSMGPLLDA
                     MMGPTGKVIITACLIVSVLASYISWTMYSAEVPYRGSKNGAFPKILDKVNKNGTPINS
                     LWFTGFIVQFCLVLVFVFEQSYNTLLLISTSMILIPYFLIGAYLFKLAIQTNATWYIK
                     LTGFMAALYGLWIVYAAGLEYLLLSVVLYVPGILLFLYSNYKFNGRLTLSTAEKVILI
                     VLLVIFGYAVIELPALLAA"
     gene            5250..6472
                     /locus_tag="PXA33_000652"
                     /pseudo
     CDS             5250..6472
                     /locus_tag="PXA33_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252970.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF711 family protein"
     gene            complement(6510..7706)
                     /locus_tag="PXA33_000653"
     CDS             complement(6510..7706)
                     /locus_tag="PXA33_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDL5120012.1"
                     /translation="MKQVIRFIVFSTLSLFSFSTFAKTIVAIDAGHGGKDPGAIGKNL
                     GILEKSVTLAISKELKALLDADPNFKAVMTRSGDYFIQLPQRTEIARKHRADLLVSIH
                     ADSSPRGDSLKGASVWVLSNSRASDEMGKWLEDHEKQSELLGGAGSVLSSNNERYLNQ
                     TVLDLQFSHSQRAGYELGKSILAKMSNVAALAKSSPQHASLSVLRSPDIPSVLVETGF
                     LSNSTEEAKLASANYRKQVARAIYNGLVAYRAKNAVASSSVSKKSDKEDKQNKKEEKT
                     AKADLTKKPASEKQEKNTQSDKTKLKEKSKKEDKKEDSAKTNKKKEQQLKEKTALEKD
                     QKNQSKKSDSQREIKVSNSGYHIVQQDETVYSIARAYGTTPQKISELNNLKNYQITVG
                     KKLKVK"
     gene            complement(7726..8217)
                     /gene="tsaE"
                     /locus_tag="PXA33_000654"
     CDS             complement(7726..8217)
                     /gene="tsaE"
                     /locus_tag="PXA33_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HDL5120013.1"
                     /translation="MTDSLSFYCEDETKLLEFGQSFALELKKYLLQDQAHSLVVYLNG
                     ELGAGKTTLTRSIVRAFGHQGNVKSPTYTLVEEYQLPPFSLYHFDLYRLADPEELEFM
                     GIRDYFKPQTLCLLEWAVKGKGMIPEADFVIQIDYKNDGRQISLLPQNQTAVDILVNF
                     HQK"
     gene            complement(8217..8771)
                     /gene="orn"
                     /locus_tag="PXA33_000655"
     CDS             complement(8217..8771)
                     /gene="orn"
                     /locus_tag="PXA33_000655"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HDL5120014.1"
                     /translation="MAQNEQNLIWIDLEMTGLDPEKERIIEIATIVTDKDLNILAEGP
                     VLAVHQTDDLLEKMSDWCVKTHTANGLIERVKASKLTERAAELQTLDFLKRWVPKGAS
                     PICGNSIAQDKRFLYKYMPDLADYFHYRHLDVSTLKELARRWKPEILDKFSKGNTHLA
                     LDDIRESINELKFYREHFIQLDQK"
     gene            complement(8781..9260)
                     /gene="coaD"
                     /locus_tag="PXA33_000656"
     CDS             complement(8781..9260)
                     /gene="coaD"
                     /locus_tag="PXA33_000656"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HDL5120015.1"
                     /translation="MSYNVIYAGTFDPITNGHLDIICKASHLFGKVIVAVAQNPSKQP
                     LFSLEERVELVKQSCTQWQNIEVIGFSGLLADFAKQQNAKALIRGIRGADDIDYEIQL
                     AQLNYKLSGDLETIFFPPSIEWRYLSSTMVREIYRHNGDISQFVPQAVKNALKIAKV"
     gene            complement(9262..10542)
                     /gene="waaA"
                     /locus_tag="PXA33_000657"
     CDS             complement(9262..10542)
                     /gene="waaA"
                     /locus_tag="PXA33_000657"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HDL5120016.1"
                     /translation="MLRLLYICLSYLLQPVVLLLMWYKGRKQPAYRKRLWERYGIYDE
                     SEKPKAKGVVIHAASVGEVIAATLLIKAVSKQYPELPLIVTTVTPTGSGRVQAAFGNS
                     VSHFYLPYDLPDAIERFLNFIDPKLMIVIETELWPNLIRKVNIRKIPFVIANARLSPR
                     SAKRYGWVKGSIKDMLNQISLIMAQDEVSRDRYLALGYAPAKMVNTGNLKFDLEITPQ
                     LHQSVIKTKASLNLTERPIWIAGSTHEGEEKLILDAHKLLLEHYPNLVLILVPRHPER
                     FGLVELLIQKSDLNYVKRTENKTLSQNTQVMLGDTMGEMMLLYGLSDIAFVGGSLVKH
                     GGHNPLEPIAFNIPVVSGLYTYNFPEIFEKLREVKGVIEIESSVEALTESIQLLLKQP
                     QIGQEIAKSGLSVLQENQGALKRHLDLLASYLEK"
     gene            complement(10722..12101)
                     /gene="yegQ"
                     /locus_tag="PXA33_000658"
     CDS             complement(10722..12101)
                     /gene="yegQ"
                     /locus_tag="PXA33_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HDL5120017.1"
                     /translation="MTLKYAKPELLSPAGSLKNMRYAFAYGADAIYAGQPRYSLRVRN
                     NEFNHANLKVAIDEAHALGKKFYVVVNIAPHNSKLKTFIKDLKVVVDMKPDALIMSDP
                     GLIMLVRENFPEMDIHLSVQANAVNWATVKFWRQMGLTRVILSRELSLEEIEEIREQV
                     PDMELEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYKMEEGKIDEVGQI
                     VPKYEPEIEVKNVAPTLGNGEATDKVFLYTESQRPDEQMTAFEDEHGTYFMNSKDLRA
                     VQHVERLTQMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDESLMDTLESL
                     AHRGYTEGFLRRHTHDEYQNYEYGYSISERQQFVGEFTGKRNAEGLAEVAVKNKFLVG
                     DSVEMMTPKGNIVFKIERMLNRKNENVDAALGDGHFVFLDVPADIELDFALLMRNLVN
                     TNTRNPHQA"
     gene            12229..12600
                     /locus_tag="PXA33_000659"
     CDS             12229..12600
                     /locus_tag="PXA33_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5120018.1"
                     /translation="MKLKYLLATTLLAFSTFTAAQSIKTEMMQMNMQANKLMRAESAE
                     EFSEIANDFIIITEKTKGIFPNSVEGDKAAQEDYQAGMQKLIDMVNQASEKAQAGELQ
                     EAKMLISELEMIKREYHKKYK"
     gene            complement(12639..16115)
                     /gene="dnaE"
                     /locus_tag="PXA33_000660"
     CDS             complement(12639..16115)
                     /gene="dnaE"
                     /locus_tag="PXA33_000660"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HDL5120019.1"
                     /translation="MSDCRFVHLKVHSDFSMINGLAKVKSLVKTAAANNMVAMGLTDF
                     INFCGLVKFYGEALGAGIKPIIGADVYVRTESGNEDYFELTLLAKNNKGYHNITQILS
                     KAYQQGYVEFPMVEREWLAELNEGIIILSGGRMGDVGKALLKENRQEAEEKLAFYQQY
                     YPNHFYLSVCRTGRADEERYIKQAVEFSQKNAIPLVAVNDVVFLKEDDFDAHEIRVAI
                     HDSYTLDDPKRPKKYSPKQYFRTEEEMCKLFADLPSALANTVEIAKRCSVTVRLGEYF
                     LPNFPTGELSTEDFLVKKSKEGLEERLEFLFPDPEERANKRSVYDERLQVELDVINQM
                     GFPGYFLIVMEFIQWSKDNDIPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFLN
                     PERVSMPDFDVDFCMDGRDRVIEHVAETYGRQAVSQIITFGTMAAKAVIRDVGRVLGH
                     PYSFVDRISKLIPPDPGMTLEKAFAAEPKLPELYEADEEVKDLIDMARKLEGVTRNAG
                     KHAGGVVIAPTAITDFSPLYCDSEGLHPVTHFDKNDVEYAGLVKFDFLGLRTLTIIKW
                     ALEMINERLAREGKEPIRIETIPLDDKKSFALLLAAKTTAVFQLESRGMKDLISRLKP
                     DCFEDIIALVALFRPGPLESGMVQNFIDRKHGKEEVSYPDPQYQHECLKPILEPTYGV
                     IVYQEQVMQIAQELAGYTLGGADLLRRAMGKKKPEEMAAQREVFEKGAEAKGIDGKLA
                     GQIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTSEMDNTDKIVG
                     FYDECMNMGLTVVPPDVNSGKHRFSVNEKGEIVYGLGAIKGVGEGPIEAILAARQKEG
                     KFKDLFDLTARVDLKKINRRTFESLIMSGAFDKLGPHRAALMKNLEDALKASDQHAKM
                     EELGQSDMFGVLTETPEEVQNAYANTPKWSEQVILDGERATLGLYLSGHPIGRFLKEL
                     SHYAPNRLNELQPTFRGQMTTVSGIVMASRVAVTKRGSRIGIATIEDRSGKLDITLFS
                     EALENFGHLMQQDQIIVATGSVQFDDFSGGLKMSAREVLSLDEARGRFAKSLALAISK
                     EQLSADFIKKMTAIITPHKEGTLPLHFYYQSPEGRVLLKGGVEWRISPNEAMFSELKA
                     LLGENSVELEFE"
     gene            complement(16340..17437)
                     /gene="lptG"
                     /locus_tag="PXA33_000661"
     CDS             complement(16340..17437)
                     /gene="lptG"
                     /locus_tag="PXA33_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HDL5120020.1"
                     /translation="MQFFSMNILERYIGKTILSAIFLTLFMLIGLGAIIKFVEEFRDV
                     GRGSYDGLKAAYYTFLTIPRDIETFFPIAALLGSLLGLGNLASLSELVVMQASGFSRF
                     RIGLAVMKTALPLVIFTMIIGEWGVPQTEQFARNMRSIAQSGGSMLATKDGFWAKDGN
                     SFIYINHIKNEKSLDNVLIYQFGEGQNKRELQSVLKARTANYSDEKGWVLSKVEKSTV
                     GENQIQQAKQPDQVWQTSLTPSKLGIVSLKPESLSISGLADYVGFLKDTGQDPKRFEI
                     TFWRKVFQPISMAVMMLLAISFIFGPLRSSTMGAKIVIGIVAGFMFYVANIVFGNLSL
                     IATWLPIPVGALIPSLLCLIIVWWLLSKKRD"
     gene            complement(17437..18546)
                     /gene="lptF"
                     /locus_tag="PXA33_000662"
     CDS             complement(17437..18546)
                     /gene="lptF"
                     /locus_tag="PXA33_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HDL5120021.1"
                     /translation="MILSRYLTKEIFKSQLAILFILLLIFFCQQLVRVLSSAVSGKVP
                     ADLVVSLLGLGMPTMAQLMLPLSLFIALLLTLGRFYAESEITVMRACGIGQGLLVKVA
                     MFLSIFTTALAVYNVFWLTPWAINKQSEMLAEAKANPRFAALSAGQFMSAGGYVLFID
                     KIENNSLNDIYVFQPEQQKKNKPSVVVAESGTLQGLPNGDQVLTLAESKRFEGTPQSA
                     DFRISHFENYSAYLGYQDVESDEKLVQRADFKKLMETKTPEAKAELHWRLALILAVPL
                     MALLAVPMSSVNPRQGRFAKLLPALLLYLIYFLLQSSLKSSGASGKLNAEVLMPLVSV
                     AFLVLGVILNAWDSKFMSKLRYQFGAKKLTNKAVA"
     gene            complement(18720..19517)
                     /locus_tag="PXA33_000663"
     CDS             complement(18720..19517)
                     /locus_tag="PXA33_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5120022.1"
                     /translation="MRFWGKTFVVGLIGLGTQNTIFANDDAQISRYEQAKLRLSYDGN
                     VGNLLQQLSQRLKVGFIAYDVDITRKISLQNETETAIKTINEQIESQLTNIDVRFEKI
                     GERLFLVVSAKDVAPLLQNEPKEVQFVGDVIFDGEPNLTEQAQSTAEATKVQNILNIA
                     TNQEKIMAAKGKKAPQYKTVTKDNIGLKNIRVTALGTFLVFDTSVDAAKLRVKGNFED
                     IAQGENIIAILHQNTEAPPKIEIEDSQGKKLILEAHKATSSKKSKKQ"
BASE COUNT         5613 a         4451 c         3805 g         5730 t
ORIGIN      
        1 ataactcttt tgggtaataa tttcatcagt taaaaaaatt atcaaattta aattttatga
       61 ggaaccaaac tatggctaaa aatacgcaac catttgatgc ccaagcagaa gtgaatgagc
      121 ttgttgaaaa aggcttaaaa gcccttgatg agtttagaaa actcaaccaa gaacaagtgg
      181 attttattgt cgctaaagcc tctgttgctg cccttgataa acacggtatt ttagcaatgc
      241 acgcttacga agaaaccgga cggggtgtgt ttgaagacaa agccactaaa aacctgttcg
      301 cctgtgagta tgttgtcaat aatatgcgac atttgaaaac cgcaggggtc attagtgaag
      361 atgatgtcac cggtattacc gaaattgccg accctgtcgg tgttgtctgc ggtatcaccc
      421 caaccaccaa cccaacctca accaccattt tcaaagcgtt aatcgccctt aaaacccgta
      481 accctatcgt ttttgctttc cacccgtctg ctcaacaatc ttctgcccac gctgctcaaa
      541 ttgtgcgtga tgctgccgtg gctgccggtg caccggaaaa ctgtattcaa tggattaccc
      601 agccttcaat ggaaggtaca tctgctttaa tgaaacaccc gggcattgca actattttag
      661 ccaccggcgg taatgcaatg gttgaagccg cttactcttg cggcaaaccg gcattaggag
      721 ttggtgcagg caacgtaccg gcttatgtag aaaaaaccgc taaattaaaa caagcggtgt
      781 atgacattgt aatgtcaaaa tctttcgaca acggtatgat ttgtgcctct gaacaagcgg
      841 cgattgtaga taaagaaatt tacgctgagt ttattaaaga gatgcaatct tacggcgtct
      901 atttagtgaa caaaaaagag aaagccttat tggaaaaata cattttcggt attgataaag
      961 ccaaagatga aaactgctca ggtgcaaaac tcaattcagc ggtggttggc aaaccggctg
     1021 cgtggattgc agaacaagcc ggctttaaag taccggaaaa aaccaatatt ctgttagccg
     1081 aatgtgcctt tgtgggcgaa gctgagccac ttacccgtga aaaactttcg cctgtactgg
     1141 ctctgttaaa atccgactcc accgaacacg gtttggaatt gtctgaagcg atggtgaatt
     1201 tccacggttt agggcatagt gccgccattc ataccgaaaa caaagcctta gccaaaacct
     1261 ttggcgaacg ggtaaaagcc attcgggtga tttggaactc accatcaacc tttggcggta
     1321 tcggtgatgt atataactcc ttcctgccat cgcttacgct gggctgtggt tcctacggta
     1381 aaaactctgt aggtaacaac gttagtgcgg tgaatttaat caatatcaaa cgtgtgggca
     1441 gacggagaaa taatatgcaa tggtttaaag tgccatcaaa aatctacttt gagcgtgact
     1501 caattcaata tttgaaatca atgcacgatg tgcataaagt aatgatcgta accgaccgct
     1561 caatggttga tctcggcttt gtggatagaa ttaccgaaca acttcgccaa cgtcgcaacc
     1621 aagtgatgat tcagttcttt accgatgttg aaccaaaccc aagcctgcaa accgtacaac
     1681 gtggtacaga attaatgcgt agcttccagc ctgatactat catcgcactt ggtggtggtt
     1741 caccaatgga tgcagccaaa attatgtggt tattctacga acaaccggaa gtcgatttcc
     1801 gtgatttagt gcaaaaattt atggatatcc gcaaacgtgc attcaaattc ccacaattag
     1861 gcagaaaagc gaaatttgtc ggcattccaa ccacctccgg aacaggctct gaagtcacac
     1921 cgtttgcagt aattaccgat ggcgatatta aatatccgct cgctgactac tcgcttaccc
     1981 caaccattgc gattgtcgat cctgcgttag taatgtctgt gccgtctcac gttgctgccg
     2041 acaccggttt agacgtatta actcacgcta ccgaggctta cacatcgatt ttagccaacg
     2101 actataccga cggcttagcc ttgcaagcca ttaagttagt gtttgaaaac ttggaaaaat
     2161 cggtcaaaga gtttgatgaa accgcccgtg aaaaaatgca caatgcttcc acaatggcag
     2221 ggatggcatt tgccaatgcc ttcttaggta tttgccactc aatggcacac aaaatcgggg
     2281 gtaaattcca caccatccac ggtcgaacca atgcgatttt actgccacac gttattcgct
     2341 ataacggcac acgcccaacc aaagtggcaa cttggccaaa atacaccaat tatgtcgcag
     2401 atgtgcgttt ccaagatatt gccagaatgc ttggcttacc ggcagaaacc ccggaacaag
     2461 cggtggaagc ttacgcccaa gcggtacacg atttagccgt tcgttgcggc gtgaaaatgt
     2521 cgcttcgtga gcaaggcgtt gatgagcaag ccttcttgaa tgcccgccgt gaacttgccc
     2581 tacacgcatt tgaagaccaa tgcacaccag caaacccacg tttggcaatg attgaagata
     2641 tggaagcgat tatgactaaa gcctactatg gcaaggaata aagcacaatt taatcgttaa
     2701 acttactaaa tggccgcttg caagcggtca tttttcttga aatttttact tttcccttca
     2761 caaaatactt tcgctctttt acaaagcata gtatttttat cgctcctttt atcttacagg
     2821 aaatactatg caaattacga ttttagacgg cggaattagc cgtgaattaa tgcgtcgcaa
     2881 tgctccattt catcagcctg aatggtctgc tgccgctttg tatgaaggcc cacattttgt
     2941 gcaagcggta cacgaagatt ttatcgctca cggggcagaa gtgattacta ccgacagcta
     3001 tgcggttgtg ccgtttcaca ttggcgaaaa acgctttgcg gcagacggca aaatgcttgc
     3061 cgatttagcc ggtcgccttg ccaaacaagc ggtcaaaaat tcaggaaata ttgcaactca
     3121 aattgcaggc tcacttccac caatgtttgg ctcttaccgt gccgatttaa ttcaaatgga
     3181 acgctttgct gaagtcgctc agcctttaat tgacgggctt tcgccttatg tggatatttg
     3241 gctttgcgaa acccaaagtg cgattattga gccgatttcc atcaaagcat tattgccgaa
     3301 agacaaccgc ccgctttggg tctcttttac cctcaccgat gatgaaccaa ccaccgagcc
     3361 acaacttcgc tcgggcgaaa gcgttaaaca agcagtcgaa gaaatggtga agttaggcgt
     3421 gcaagcgatt ttatttaact gctgtcagcc tgaagtgatc ggcgaggcat taagtgtaac
     3481 ccaaaaaact ttagccgaat taaaagctac ccatattcgc acaggagctt atgccaacgc
     3541 ctttgctcca caaccaaaag atgccaccgc caatgacggt ttagatgaag ttcgccaaga
     3601 tttaaccctg gaggcttatt taggctgggc aaaaaaatgg acggaacaag gggcaactat
     3661 tatcggtggc tgctgcggca ttggggttga gtatattgaa acgctggcaa aaaatttaaa
     3721 ataaggaaat cgaatgtcaa ataaaaaaat aggattaatt tcactcaccg ccttagtgct
     3781 aagctcaatg attggctcgg ggattttcag cttaccgcaa aatatggcag ccgttgccgg
     3841 agcagaagcc attaccattg gctggctgat taccggtgtc ggcattatct ttttaggttt
     3901 atcgtttttc tttatttccc gcttgcgtcc ggatttagac ggtggcattt acacctacgc
     3961 acgggagggt tttggcgagc tgatgggctt tatgtcagcg tggggctatt ggctctgtgc
     4021 caccattggg attgtaggat acctcaccgt tgcttttgaa ggtttgggtg tatttaccga
     4081 caacccgaac accgtgattt tcgggcaagg taacaccctc gcctctttta ttggatcttc
     4141 aatcattgtg tggctggttc acgccctcat cgcaggcggt atcaaagaag ctgcctttgt
     4201 aaacttaatt gccacttttg tgaaagtcgc tccgctcatt ctgtttatcg ggttaggcat
     4261 ttggtatttc gatgtggatc tgtttaatag cgatgttaaa gccaccgcac ttaataatag
     4321 tgtaagcgac caagtgaaaa gcacaatgtt aatcacctta tgggtattta ccggtgtgga
     4381 aggggcttct gtgctgtcgg ctcacgcgaa aaagcgttct gacgtgggac tggcaaccgt
     4441 attaggtatt attattgcac ttgctcttta tgtggcaatt accattcttt cactcggaat
     4501 tctgccacgg gaaactattg ccaatatggc aaatccgtca atggggccgt tgctcgatgc
     4561 aatgatgggc ccaaccggca aagtgattat tactgcttgt ttaattgtat cggtgcttgc
     4621 ctcttacatt agctggacga tgtattcggc agaagtgcct tatcgtggct caaaaaacgg
     4681 ggctttcccg aaaattttag acaaagtgaa taaaaacggc acgccgatta actcactctg
     4741 gtttaccggc tttatcgtgc aattttgctt ggtgttggtg tttgtttttg agcaaagcta
     4801 taacacctta ttgctgattt ccacctcgat gattttaatt ccctatttct taatcggggc
     4861 ttatttattc aaattagcga ttcaaaccaa cgccacttgg tacattaaac tcaccggctt
     4921 tatggcagcc ctttacggat tatggattgt ctatgccgca ggactagaat atttgctgct
     4981 ttcggtggtg ctttatgtac ccggaatttt actcttcctc tactccaact ataaattcaa
     5041 cggcagatta accctttcaa ccgctgaaaa ggtcatctta atcgtgctac tggtaatttt
     5101 tggctatgcc gtgattgagc tgccagcact attagcagca taattcccat caaaaatgac
     5161 cgcttgcaag cggttatttt tctaggatct tctgcaaaga ttcattagaa tagaaacaac
     5221 gtcaataata aataaaaaga ggagaaacaa tgcaaactta tcaacataaa gccctttgtc
     5281 gggtaagaac cattaccctt tttctgagtt taaccaaaga taaattgcag tgggaaacgg
     5341 cattaaatgc ggcaaaagca gaatttgatc tattagttcc cgctattcaa acacaaggct
     5401 atcacataca atcgattcga atcgttacca acagtttcgg ggaatatctt gatctcacta
     5461 atattgaaac agcgaaacag gatttagcct atcttaaaca gctattaacc caacttaacg
     5521 atagcggttt acgcattcgc tttgccattg gtgaggcaaa aacaccccac gaattagcat
     5581 tactacctga gctgatttta aattatggtg atttatctaa cgcttgtgtc aatattgaac
     5641 ccgatgaatt tgggatttta gacaatgaaa ccattttaca atctgctcaa attatcaaac
     5701 aaatcgctga aattacccca agaggtgaag gcaattttaa ttttacggtg aattttaact
     5761 gtaaaccttt tattccttat tttccggcaa gctaccattc aagcaagctc cctaacagtt
     5821 ttgtgatagg cctagaaaca ccagatctat tagtagcggt gttaaaagag ctgaaagcca
     5881 gcccacataa cgacttttat cgccaatgct atgaaacgat gtctcaagcc ttgcaatatc
     5941 acgttgatga agttctcgaa gcagtaaaat cggtacaaca caaattacaa tttgcctttg
     6001 ctggcattga tagttctgct gctccgtcaa aaactgtgcc tcaatgacag aagtttatga
     6061 attaatgggc gtaccatttt ttggggcagc cggaacagta gaagcctctt cacttctgac
     6121 aaaagtcttt aaatctatca aacatgtacc gcttgtcggt ttttcaggtt taatgttggc
     6181 agtaaccgaa gatttaggct tagccgcagg cacacacaaa gcccaattcg acattcgtgc
     6241 tttactaact tatagtgctg tttgtggcat tgggttagat accgtaccga tttccaatga
     6301 agccactgtt gaacaaattg ccgcattaat gcgagacacc ggcacaatgg catttagact
     6361 aaataaaccg cttacagtac gtttatttcc aattccgaat aagagtgctg gggaagtgac
     6421 agaatttgaa agtgatgatt tatgtaactg ccgaatttta gctgtacctt aaaataaagg
     6481 ctgatggata tttccatcag cctttcaatt tacttcacct tcaatttttt acctaccgtg
     6541 atctgataat ttttcaaatt attcagctca ctgatttttt gcggcgtagt accgtaagct
     6601 ctagcaatcg agtaaaccgt ttcatcttgt tggacaatat ggtagccact gttagacacc
     6661 ttaatttctc tttgagagtc tgatttttta gactgatttt tttgatcttt ctcaagagct
     6721 gttttttctt ttagttgctg ctcttttttc ttattggttt tagccgaatc ttctttttta
     6781 tcctcttttt tgctcttttc tttcaatttt gtcttatctg attgcgtatt cttctcttgt
     6841 ttctcagaag caggcttttt agttaaatca gctttagcag ttttttcctc tttcttattt
     6901 tgcttatctt ctttgtccga ttttttactg accgaagaac tagcaactgc attttttgcc
     6961 cgataagcga ctaatccatt ataaatggcc cgagcaactt gtttacgata atttgcactg
     7021 gctagctttg cttcctcggt tgaattagat aaaaatcccg tttccaccaa tacagatgga
     7081 atatcaggtg aacgcagaac acttaagctt gcgtgctgag gagacgattt tgctaatgca
     7141 gccacatttg acattttagc taaaatgctc ttgcctaatt catagccggc acgttgagag
     7201 tgagagaact gtaaatcaag cactgtttga tttaaataac gttcattgtt gcttgaaagc
     7261 accgaaccag ccccgccaag taattccgac tgcttctcgt ggtcttccaa ccatttaccc
     7321 atttcatcgc tagcacggct attagaaagt acccaaaccg aagccccttt caaggaatcg
     7381 ccacgaggcg aagagtctgc gtgaatagaa accagcagat cggcacgatg cttacgggca
     7441 atctcggtac gttgcggtaa ttggataaaa taatctcccg agcgtgtcat cactgcttta
     7501 aaattaggat cggcatctaa caaggctttt agctctttag aaattgcgag ggtaacgctt
     7561 ttttccaaaa tccccagatt tttaccaata gcccccggat cttttccccc gtgtccggca
     7621 tcaatcgcca caatggtttt ggcaaatgtg gaaaaactga ataaactcaa tgtgctaaaa
     7681 acaataaatc tgataacttg cttcattacc ttccctacaa gcggtctatt tttgatgaaa
     7741 atttactaat atatctactg cagtttgatt ttgcggcaac aggctaatct gccgtccgtc
     7801 attcttataa tcaatttgga tcacaaaatc agcctctgga atcattcctt tacctttaac
     7861 tgcccattca agcaaacaaa gcgtttgtgg tttgaaataa tcacgaattc ccataaactc
     7921 cagctcttca ggatcggcta agcgatacaa atcaaaatgg taaaggctga aaggcggaag
     7981 ttggtattct tccaccagag tataagttgg gctttttaca ttgccttgat gcccgaaagc
     8041 ccgtacgatc gagcgggtta aggtggtttt acctgcccca agttcgccgt ttaggtaaac
     8101 aaccagcgaa tgagcttggt cttgcaataa atatttctta agttctaacg cgaatgattg
     8161 accaaattcc agcaatttag tttcatcttc gcagtaaaaa gagagagaat ctgtcattat
     8221 ttttggtcta gctgaataaa atgttcccga taaaatttca attcgttaat ggattcacga
     8281 atatcgtcca acgctaaatg cgtattgcct ttactgaatt tatctaaaat ctccggtttc
     8341 caacggcgtg ccagctcttt taaagtactc acatctaagt ggcgataatg gaaataatcg
     8401 gctaaatcag gcatatattt atacaaaaaa cgtttatctt gagcaatgct gttgccgcaa
     8461 atcggagaag cgccttttgg cacccagcgt tttaagaagt ctaaagtttg caactctgct
     8521 gcccgttcag tcaatttgct cgctttcact cgttcaatca gcccgttagc ggtatgggtt
     8581 ttgacgcacc aatcactcat tttctctaat aaatcatcgg tttggtgtac cgcaagtacc
     8641 ggtccttccg ctaaaatatt taaatcctta tcggtcacaa tggtggcgat ttcaataatg
     8701 cgttcttttt ccggatctaa ccctgtcatt tcaagatcga tccaaattaa attttgttcg
     8761 ttttgagcca ttttatttcc ttacactttt gcaattttta gggcattttt gaccgcttgc
     8821 ggcacaaatt ggctgatgtc accattgtgg cgatagattt cccttaccat cgttgaagaa
     8881 aggtaacgcc attctattga agggggaaaa aaaatagttt ctaaatcgcc cgataatttg
     8941 taatttaact gagcgagttg gatttcatag tcaatatcat ctgccccacg aataccacgg
     9001 attaacgctt tggcattctg ctgtttggca aagtctgcta acaatccact aaagccaatc
     9061 acctcaatat tttgccactg ggtacagctt tgtttaacaa gctccactcg ttcttcaaga
     9121 ctaaaaagcg gctgcttgct tgggttttga gcaacggcaa caatcacttt tccaaataag
     9181 tgagaggctt tgcaaattat gtccaaatgc ccattggtaa tgggatcaaa ggtgcctgca
     9241 taaattacgt tataactcat tttatttctc taaataggag gctaataaat ccaaatggcg
     9301 ttttaatgcc ccttgatttt cctgtaacac gcttaagccg gattttgcaa tttcttgtcc
     9361 gatttggggc tgcttaagca ataattgaat gctctcggtc aaagcctcga ctgaactttc
     9421 aatttcaatc acgcctttca cctcacgcaa tttctcaaag atttcaggga aattataggt
     9481 gtaaagccca gaaaccaccg gaatattaaa agcgattggt tcaagtggat tatgcccacc
     9541 gtgtttgacc aaactgcctc ctacaaaagc aatgtcagac aacccataca gtaacatcat
     9601 ttcccccatt gtatcaccta acattacttg agtattttgg cttaacgttt tattctcagt
     9661 acgttttaca taatttaaat ctgatttttg aatcagtagc tcaactaaac caaaacgctc
     9721 cggatggcga ggcactaaaa tcaacactaa attcgggtaa tgctctaata agagtttatg
     9781 agcatctaaa atcagcttct cttccccttc gtgagtactg ccggcaatcc aaatcggtct
     9841 ctctgttaaa ttcagactag ctttagtttt aatcaccgat tgatgaagct gtggcgtaat
     9901 ttctaaatca aatttcaggt taccggtatt aaccattttt gccggtgcat aaccgagtgc
     9961 taaatagcga tctcggctta cctcatcttg agccataatc agactaattt ggttgagcat
    10021 atccttaatg ctacctttta cccagccata acgcttcgca gaacgtggcg ataagcgggc
    10081 attggcaatc acaaatggaa ttttacgaat atttacttta cgaattaaat tcggccaaag
    10141 ttcggtttca attacaatca tcaatttcgg atcgataaaa ttcaaaaacc gctcgatagc
    10201 atcaggtaag tcataaggca aataaaaatg tgaaacacta ttaccaaaag cagcttgcac
    10261 acgcccagag cctgttggcg ttaccgttgt gactatcaac ggaagttcag gatattgctt
    10321 gctgactgcc ttaatcaaaa gcgttgcagc aatcacctca ccaaccgatg ccgcatggat
    10381 taccacccct tttgctttcg gtttttccga ttcgtcataa atcccataac gctcccataa
    10441 acgtttccga taagccggtt gtttacgccc tttgtaccac attaatagca gaaccacagg
    10501 ctgtaataga taacttaaac aaatatagag taaacgtagc atttattttc cttataccat
    10561 aaccctcacc tcttgtggga gagagtcaaa tacgccaata aatcgttcct ctcttgttcg
    10621 agagggggga acaagcggtt atttttacca aaaattttgc aatctatatc ctcaaagaca
    10681 aaaccacgga ataaccttaa tccgtggttt atctcgcatc attacgcttg atgtggattg
    10741 cgggtattcg tattcactaa gttacgcatt aaaagtgcaa aatccaactc aatatctgcc
    10801 ggcacatcta aaaagacaaa atgcccgtca ccgagtgctg cgtccacatt ttcatttttg
    10861 cggttaagca tacgctcgat tttaaataca atgttgcctt taggggtcat catttccact
    10921 gaatcgccca ctaagaattt gtttttcact gccacttccg ccaagccttc tgcattgcgt
    10981 ttgccggtaa attcgcccac aaattgttgg cgttctgaaa tcgaatagcc atattcatag
    11041 ttttggtatt catcgtgagt atgacgacgt aagaagccct ctgtataacc acggtgtgca
    11101 aggctttcaa gagtatccat taaactttcg tcaaacggtt taccggcagc ggcatcatca
    11161 atcgccttac gatacacctg agccgttctc gcacagtaat agaacgattt cgtacgcccc
    11221 tcaattttca gtgaatgcac ccccatttga gttaaacgct caacgtgttg tactgcacgc
    11281 agatctttag agttcataaa ataggtgccg tgttcatctt caaaggcggt catttgctca
    11341 tcagggcgtt gtgattctgt ataaaggaac actttatctg ttgcttcgcc attacctaga
    11401 gtaggagcca catttttgac ttcaatttcc ggctcatact taggcacaat ttgccctacc
    11461 tcatcaatct taccttcttc cattttatat tcccaacggc aagcattggt gcaagtgcct
    11521 tggtttgggt cacgcttatt gatatagcct gaaagcaagc agcgacctga atatgccata
    11581 cacaaagcac cgtgaacgaa gatctcaagc tccatatccg gcacttgttc acgaatttct
    11641 tcgatctctt cgagtgataa ttcacgggat aaaatcacac gggttagccc catttgacgc
    11701 cagaatttca ccgttgccca gtttaccgca ttggcttgca ccgaaagatg aatatccatt
    11761 tcagggaaat tttcacgcac caacataatt aagcccggat cagacataat caaagcgtcc
    11821 ggcttcatat ccactaccac ttttagatct ttgatgaagg tttttaattt tgagttgtgt
    11881 ggcgcaatgt ttaccaccac atagaatttt tttcctaagg cgtgggcttc atcaatcgca
    11941 acttttaaat tggcgtggtt aaattcatta ttacgcacac gcaagctata acggggctgt
    12001 cctgcataaa tcgcatcggc accataagca aaagcgtaac gcatattttt taaagaacct
    12061 gcaggcgaaa gtagctcagg tttggcatat tttaaagtca tattattctc ttttctgata
    12121 acaagtcagg acttctcgac gtgagaagcg taagcggtat attttctaag aaatattgcg
    12181 tgatggcaag ctaatccatt acaatccgat ttttatgtgg ggaacaatat gaaacttaaa
    12241 taccttttag caacaacatt actggctttt tcaacattta ccgccgctca aagcatcaag
    12301 acggagatga tgcaaatgaa tatgcaagcg aataaattga tgcgtgccga atcggcagaa
    12361 gagtttagcg aaattgcgaa tgatttcatt ataattaccg agaaaaccaa agggattttt
    12421 cccaatagcg tagaaggcga taaagcagct caggaagatt accaagccgg tatgcaaaaa
    12481 ttaattgata tggttaatca agccagtgag aaagctcaag caggtgaatt gcaagaagca
    12541 aaaatgctga tttccgagct tgaaatgatc aagcgtgaat atcacaaaaa atacaaataa
    12601 ttcaacacat taaaagggtg agctaaagct caccctttct attcaaattc cagttcaacc
    12661 gaattctcac caagaagtgc ctttaattcg ctaaacattg cctcattcgg gctgattcgc
    12721 cactctactc cgcctttgag caatacccta ccttccgggc tttggtaata aaaatgcagc
    12781 ggtaaagtgc cttctttatg tggggtgatg atagcggtca tttttttaat aaaatctgca
    12841 gaaagttgct ctttgctaat cgctaacgct aaacttttgg caaatcgacc ccttgcttca
    12901 tcaagggata acacttctcg agccgacatt ttcagtccac cgctgaaatc atcaaactgt
    12961 accgaacctg ttgccacaat aatttggtct tgttgcatca aatgcccgaa attttctaag
    13021 gcttccgaga aaagggtaat gtccaatttc cccgagcgat cttcaatagt cgcaatccca
    13081 atacggcttc ctcgcttggt aactgccact cgagatgcca taacaattcc tgaaaccgtg
    13141 gtcatttgcc ctcggaaagt cggttgcaat tcattcagcc gattaggggc atagtgggat
    13201 aattctttga ggaatctacc aatcgggtgt ccgcttaaat acagcccgag cgtggcacgc
    13261 tctccatcta aaatcacttg ctccgaccat tttggcgtat tggcgtaagc attttgcact
    13321 tcttccggtg tctcggtaag cacgccaaac atatcgctct gcccgagttc ctccatttta
    13381 gcgtgctgat ctgaggcttt aagtgcatct tccaaattct tcatcaaggc agctcggtga
    13441 gggcctaatt tgtcaaaagc accggacata atcaggcttt caaaggtgcg gcggttgatt
    13501 tttttcagat cgacccgagc ggttaaatca aatagatctt taaatttccc ctctttttgg
    13561 cgagctgcca gaattgcctc aatcggtcct tccccaacgc ctttaattgc tcctagcccg
    13621 taaacaattt cgcctttttc atttacgcta aaacgatgtt taccgctgtt tacatcaggc
    13681 ggcacaacgg ttaagcccat attcatacac tcatcgtaga acccgacaat tttatcggta
    13741 ttatccatct ctgaggtcat taccgcagcc ataaattctg ccggataatg ggcttttagc
    13801 caaagtgttt ggtaagaaac caacgcataa gctgctgagt gagatttatt aaagccgtag
    13861 ccggcaaact tttctaccaa gtcaaaaatt tgaccggcaa gtttaccgtc aatccctttg
    13921 gcttccgccc ctttttcaaa cacttcacgc tgtgcagcca tttcctccgg tttcttctta
    13981 cccattgcac ggcggagtaa gtccgctcca ccgagtgtat aaccggcaag ctcttgtgca
    14041 atttgcataa cctgttcttg gtaaacaatg accccataag tcggctcgag aataggtttt
    14101 aagcattcgt gttggtattg cggatcagga taagaaacct cttctttgcc gtgtttacgg
    14161 tcaataaagt tctgcaccat acctgattcc agcggccctg ggcggaatag tgccaccaat
    14221 gcgataatat cttcaaaaca gtccggcttc aggcgagaaa ttaagtcttt cattccccgt
    14281 gattcaagct ggaatactgc tgtagtttta gctgccagca acagagcaaa ggatttttta
    14341 tcatcaagcg gaatggtttc aatccgaatc ggctctttgc cttcacgagc caaacgttcg
    14401 ttaatcatct ccaacgccca tttgatgatg gtgagcgtac gcaagcctaa gaagtcaaac
    14461 ttcaccaatc ctgcatattc cacgtcattt ttatcaaaat gggtaaccgg gtgtaagccc
    14521 tcgctatcgc aataaagcgg cgaaaaatcg gtaatcgcag ttggggcgat caccacacca
    14581 ccagcgtgtt tacctgcatt acgagttaca ccttcgagct tacgagccat atcaataagg
    14641 tctttcacct cttcatcggc ttcatatagc tcaggcaatt ttggctcagc ggcaaaggct
    14701 ttttcaagcg tcattcctgg atcgggcgga atgagtttgg agatgcgatc cacaaagcta
    14761 taggggtgtc caagcactcg ccctacatct cgaatgaccg ctttcgccgc catagtaccg
    14821 aatgtgataa tttgcgatac cgcttgtcgc ccgtaggttt ccgcaacgtg ttcaatcaca
    14881 cggtctcgcc cgtccataca gaaatcaacg tcaaaatcgg gcattgaaac acgctcaggg
    14941 ttgagaaaac gctcaaaaag caaatcaaat tccaatgggt caagatcggt aatcttcaac
    15001 gcataagcca ccaatgagcc tgcccctgag ccacgacccg gtcctaccgg aatatcatta
    15061 tctttggacc attggataaa ctccatcacg atcaaaaagt aacccgggaa ccccatttgg
    15121 ttgatcacat ctaattcaac ttgcaatctt tcgtcataaa ccgaccgctt gttcgccctt
    15181 tcctcaggat cagggaatag aaactctaaa cgctcttcta agccttcttt cgattttttt
    15241 accaagaaat cttcggtgga aagctcaccg gtcgggaaat ttggcaggaa atactcgccc
    15301 aaacgcacgg taaccgaaca gcgtttcgca atttcaaccg tgtttgccaa tgcactcggt
    15361 aaatcggcaa acagcttgca catttcctct tcagtacgga aatattgttt cggtgagtat
    15421 tttttcgggc gtttcgggtc gtctagggtg tagctgtcgt gaatggcaac ccgaatttcg
    15481 tgtgcgtcaa agtcatcttc tttcaagaag acaacatcat ttaccgccac taacggaatt
    15541 gcattttttt gtgaaaattc gaccgcttgt ttgatatagc gttcttcgtc tgcccgtccg
    15601 gtacggcaaa cggaaaggta aaaatgattc gggtaatatt gctgataaaa agctaatttt
    15661 tcttcagctt cttgtcggtt ttctttgagt aaggctttac ccacatcgcc cattcgcccg
    15721 ccggaaagaa tgatgatgcc ctcattcagc tctgctaacc attcacgttc caccatcgga
    15781 aattccacat agccttgctg ataggctttg gacaaaattt gggtaatatt atggtagcct
    15841 ttgttgttct tcgccagtaa tgtcagctca aaataatctt cattgccgct ctcggttcta
    15901 acataaacat ctgccccgat aatcggctta atccctgctc ccaatgcttc gccatagaat
    15961 ttcaccaacc cgcaaaagtt tataaaatcc gttaagccca ttgccaccat attattagcg
    16021 gcagcggttt taactaagga ttttacctta gctaaaccat taatcattga gaaatcactg
    16081 tgaactttta aatgaacgaa acgacaatct gacataggaa tataatctaa gtaaagaagt
    16141 ttcccctctt tagtaaagac tgtcaggttc taaaaagtga agccttctgt gaataatccc
    16201 gttgggcgaa tgttattcgc ccctacaaac gagcattatt cttgctcaga gttacattta
    16261 aaatccgaat gtaggggcga attacattcg ccctttcaga acctaacaac cctgctttat
    16321 ctaaagaagg gagttaaaat taatcacgtt ttttgcttaa cagccaccaa acgatgatca
    16381 aacagagtag gcttggaatt aatgctccaa ccggtatagg taaccaagtg gcaattaagc
    16441 ttaaattgcc aaacacaata tttgccacat agaacataaa gccggcaaca ataccaatca
    16501 caattttcgc ccccatcgta ctactacgaa gcggcccgaa aataaaggag atcgcaagta
    16561 gcatcatcac cgccattgag attggttgga atacttttcg ccagaaggtg atctcaaaac
    16621 gtttcggatc ttgtccggta tcttttaaga agccgacata atctgccaag cctgaaatcg
    16681 agagcgactc cggtttgagt gaaacaatgc ctaatttgct aggggttaaa ctggtttgcc
    16741 aaacttgatc tggctgttta gcttgctgga tctgattttc tcccacagtc gatttttcca
    16801 ctttagatag cacccagcct ttttcatcac tgtaattcgc agttcgggct tttaaaacag
    16861 attgcaattc acgtttgttt tgtccttcgc cgaattgata aatcagtacg ttatctaagc
    16921 ttttttcatt tttaatatga ttaatataga taaatgaatt gccatctttc gcccaaaacc
    16981 cgtcttttgt ggcaagcatt gagccgccac tttgggcaat cgaacgcata ttacgggcaa
    17041 actgctcggt ttgtggcacg ccccattcgc cgataatcat cgtaaaaatg actaacggta
    17101 aggcagtttt catcacggct aaaccaatgc ggaaacggga aaaaccggag gcttgcatca
    17161 ccaccaattc actaaggctc gctaggttgc ctaaaccgag taatgagcca agtaatgccg
    17221 caatcgggaa aaaggtttcg atatctcgtg gaatagttaa aaaagtatag tatgccgctt
    17281 ttaagccatc gtaagatcca cgtcctacat cacgaaactc ttccacaaat ttaataatcg
    17341 ccccaagccc aatcaacata aacagggtta aaaaaatcgc ggataaaatc gttttgccga
    17401 tgtagcgttc taaaatattc attgagaaaa attgcattat gccaccgcct tattggttaa
    17461 tttttttgca ccaaattgat agcgtaactt agacataaat ttactatccc acgcatttaa
    17521 aataacacct aaaactaaaa aggcaactga taccaacggc attaacactt ccgcattcaa
    17581 cttgcctgat gcccctgacg attttagcga gctttgtagt aagaaataga ttaaataaag
    17641 caataacgcc ggcagtagtt tagcaaaacg cccttgacga gggtttacac tactcattgg
    17701 caccgctaat aatgccatta acggtacagc taaaatcaaa gcaagccgcc aatgaagctc
    17761 ggcttttgct tccggcgttt tggtttccat taatttcttg aaatcggctc gttgcactaa
    17821 tttctcatca gattcaacgt cttgatagcc taaataagca gaataattct caaaatgtga
    17881 aatacggaaa tcggcacttt gtggtgtacc ttcaaaacgc ttgctctctg ccaaggttaa
    17941 gacttgatcc ccatttggca aaccttgcaa cgtgccggat tcagcaacaa ccactgaagg
    18001 tttgtttttc ttctgctgtt ccggctggaa aacgtagata tcatttagac tgttattttc
    18061 aattttatca ataaataaca catagccacc ggcagacata aattgtccgg ccgaaagagc
    18121 cgcaaaacgt gggttggctt tcgcttccgc taacatttca ctctgtttat taattgccca
    18181 aggcgtaagc caaaacacgt tataaaccgc taatgcagtg gtaaaaatcg agagaaacat
    18241 cgccacttta accaatagcc cttgcccgat accacaagcc cgcattacgg taatttcact
    18301 ttcggcataa aaccgcccga gggttaataa caaggcgata aacagtgaga gtggcagcat
    18361 taattgtgcc attgtcggca tacctaagcc taataagctc accactaaat ctgccggtac
    18421 tttaccactc acagcagaac ttagaacacg cactaattgc tgacaaaaaa agatcagtaa
    18481 taagataaac aatatcgcca attggctttt aaatatctct ttggttaaat atcggcttaa
    18541 gatcacgttt tttctctata acatttacaa atctgaatac cgctaaacac aagcggtcta
    18601 tttttgttaa ttttttgcaa agagcgcaaa ataacttatt cgaatcgcta ataatacctt
    18661 tttcatacaa aaaaatcacc tatagaaaat atcacgcacg gcaaaaccgt gcgtaagtat
    18721 tattgttttt tcgatttttt cgatgatgtt gctttatgag cttcaagaat taatttctta
    18781 ccttggctat cttcaatttc aatcttaggt ggtgcttccg tattttgatg aaggatagct
    18841 ataatatttt caccttgtgc aatatcttca aagtttcctt tcacacgtaa cttagccgca
    18901 tcaacactag tgtcaaatac taagaatgta cctaatgcgg taactctaat atttttaagc
    18961 cctatattgt ccttcgttac cgttttatat tgaggtgctt ttttcccttt tgcagccata
    19021 atcttctctt gattagtcgc aatattaaga atattttgaa ctttagtcgc ttctgctgta
    19081 gattgtgcct gttctgttaa atttggttct ccatcaaaaa taacatcccc aacaaattgc
    19141 acctctttag gctcattttg caataatggt gcaacgtctt tagctgaaac aaccaaaaat
    19201 aaccgttcac ctattttttc aaaacggaca tcaatattgg ttaattgtga ttcaatttgt
    19261 tcattaattg tcttgatcgc agtttctgtc tcattttgaa gcgatatttt acgtgtaata
    19321 tcaacatcat aagcaataaa accaactttt aaacgttgtg ataactgctg aagtaaattg
    19381 cccacatttc catcataaga aagacgtaac tttgcctgct catagcgact aatttgagca
    19441 tcatcattag cgaaaatagt gttttgagtc cctaagccga ttagaccaac aacaaacgtt
    19501 ttaccccaaa agcgcatcat ttttatctcc aattattctc ttaaatctaa aaaactcttt
    19561 tagtataaag taagcaaact aaaagaaaaa ccagtaaaa
//
LOCUS       DANLRK010000025        19329 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.025, whole genome shotgun sequence.
ACCESSION   DANLRK010000025 DANLRK010000000
VERSION     DANLRK010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 19329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19329
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.025"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            146..394
                     /gene="rpsP"
                     /locus_tag="PXA33_000664"
     CDS             146..394
                     /gene="rpsP"
                     /locus_tag="PXA33_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HDL5120023.1"
                     /translation="MVTIRLTRGGAKKRPFYQIVVVDSRSPRDGRFIERIGFFNPLAA
                     GQAERLRLDVAKVDAWVAKGADLSDRVASLVKEARKAA"
     gene            427..954
                     /gene="rimM"
                     /locus_tag="PXA33_000665"
     CDS             427..954
                     /gene="rimM"
                     /locus_tag="PXA33_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005626427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HDL5120024.1"
                     /translation="MSEQKIEVVGKLGSTYGIRGWLRLYSSTEQTESIFDYQPWFLKI
                     KGQWQQVELESWRYHSNDLIVKLKGTEDREAAQLLTNAEIGVDLSVFPELEEGDYYWH
                     DLIGCSVVNLEGYTMGTVAEMMETGSNDVLVVRNNGKDAFGKQERLIPFLYEQVVKRV
                     DLATKTITVEWDAGF"
     gene            999..1754
                     /gene="trmD"
                     /locus_tag="PXA33_000666"
     CDS             999..1754
                     /gene="trmD"
                     /locus_tag="PXA33_000666"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HDL5120025.1"
                     /translation="MWIGIISLFPEMFKAITDFGVTGRAVKQNLLQVECWNPRDFTFD
                     KHKTVDDRPYGGGPGMLMMVQPLRDAIHAAKKAALAVEGGEAKVIYLSPQGRKLDQNG
                     VQTLASNQKLILICGRYEGVDERLIQTEVDEEWSIGDYVLTGGELPAMTLIDAVARFV
                     PGVLGKQASALEDSFAEGLLDCPHYTRPEVLDGMPVPEVLMSGHHEQIRKWRLEQSLE
                     RTWLRRPELLDSLALTDEQRVLLAKIKKQHKIS"
     gene            1781..2131
                     /gene="rplS"
                     /locus_tag="PXA33_000667"
     CDS             1781..2131
                     /gene="rplS"
                     /locus_tag="PXA33_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HDL5120026.1"
                     /translation="MSNIIKQLEQEQLKQDLPSFRPGDTLEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTLRKVSNGVGVERVFQTHSPVIDSISVKRKGAVRKAKLYYLRE
                     RSGKSARIKERLGD"
     gene            2287..2643
                     /locus_tag="PXA33_000668"
     CDS             2287..2643
                     /locus_tag="PXA33_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5120027.1"
                     /translation="MKQLSIRLPDDEFTFIKTFASAHKLTLQNFVMSAIQAKVAEMSA
                     KNKISEESNIHQPLSKDEKIMLNEAKVFYSARSHLKAENVILPSDFMEQIKSGCGAEK
                     MAEMMGYTKEDWESMK"
     gene            2645..2968
                     /locus_tag="PXA33_000669"
     CDS             2645..2968
                     /locus_tag="PXA33_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5120028.1"
                     /translation="MGQITFSNIFTKVIFENVLSASTKEKIKQMLFQSVVALPPLHSE
                     RKMILQLKKQKITIFYQVIEEQSVQILAYQFGGTDKLTGVSKAHFKTLDAIFQMTDEE
                     KEGKF"
     gene            complement(2978..3310)
                     /locus_tag="PXA33_000670"
     CDS             complement(2978..3310)
                     /locus_tag="PXA33_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HDL5120029.1"
                     /translation="MQIEQYQQWVRQFYQQQGWYERSPFMRVTYLAEEIGEVACAVRA
                     IEIGRERPDETEASQQQKRDNLIEELGDVMDNLFVLADKYGISMTEVLERHQAKFEKR
                     YLQKNMEK"
     gene            complement(3403..4599)
                     /locus_tag="PXA33_000671"
     CDS             complement(3403..4599)
                     /locus_tag="PXA33_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDL5120030.1"
                     /translation="MNIPFCLPENISPETFLREYWQKRPLLIRNGLPQIVGLFEPEDI
                     LELALEEEVTARLIQCENEKWSVKSSPLVEEDLEELPVQWSVMVQNLEQWSPELGELW
                     QAFGFIPQWQRDDIMVSCSPLNGTVGKHYDEYDVFLVQGYGQRRWQLGKWCDPSTEFK
                     PNQPIRIFDDMGELVMDEVMNPGDVLYVPSRMAHYGVSESEQGLTFSFGLRYPNATDL
                     LENLCKTIEQQADNINSSEFHLPFRLTPNEQPNALLDPKMVKMFKHQLIDLLQNSEQF
                     DELLTHSVATAASSRRYELLQTDNEYYPDEVQEILEAGGWLQQDANVKILYTENPQRV
                     YVNGEWIDELNEAEMALLIRIANGDSISWNKLASKVQDQAELELMLDTICDWLDSGWI
                     ILSEAE"
     gene            complement(4748..6091)
                     /gene="accC"
                     /locus_tag="PXA33_000672"
     CDS             complement(4748..6091)
                     /gene="accC"
                     /locus_tag="PXA33_000672"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015431873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HDL5120031.1"
                     /translation="MLEKVVIANRGEIALRILRACKELGIKTVAVHSTADRELKHVKL
                     ADETICIGPAPSVKSYLNIPMIIAAAEVTNADAIHPGYGFLSENANFAEQVEKSGFIF
                     IGPTADVIRLMGDKVSAINAMKKAGVPCVPGSDGPVGNDPKKNKEIAKRIGYPVIIKA
                     SGGGGGRGMRVVHHEKDLEESIAMTKAEAKAAFNNDMVYMEKYLENPRHIEIQVLADT
                     HGNAVYLAERDCSMQRRHQKVVEEAPAQGITPELRQFIGERCANACREIGYRGAGTFE
                     FLYENGEFYFIEMNTRLQVEHPVTEMITGVDLVKEQLRVAAGLPLSITQDDIKVKGHA
                     IECRINAEDPNTFLPSPGKITRLHVPGGLGVRWDSHIYSGYSVPPHYDSMIGKLITFA
                     ENRDIAIRRMENALSETIVEGIKTNIPLHTQILADKEFRKGGTNIHYLEKKLGLK"
     gene            complement(6163..6630)
                     /locus_tag="PXA33_000673"
     CDS             complement(6163..6630)
                     /locus_tag="PXA33_000673"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HDL5120032.1"
                     /translation="MDIRKIKKLIELVEESGITELEVSEEEGTVRISRAAPAVAPAAV
                     QYAAAPVVAPAAPQVASTVPVAAPAEAPKAAETSGHAVLSPMVGTFYRSPSPDAKPFV
                     EVGQTVKIGDTLCIVEAMKMMNRIESDKAGVVKEILVNDGEPVEFDQKLFIIE"
     gene            complement(6883..7665)
                     /locus_tag="PXA33_000674"
     CDS             complement(6883..7665)
                     /locus_tag="PXA33_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005609325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDL5120033.1"
                     /translation="MAVKNEQFIVICNGVFRLFGNIVKILSYPFHALFPKKRFTIPEY
                     SPAKIQSSIPSKITKTIWQTNYSNKVTLPMYANYLFNRLMSLSYDYRYVSTEEREAYI
                     KANADERTFNAYSKLTDGAAQADFWRIFTLLREGGIYIDIDGHLVYPLSKIIGENDSE
                     VLIKRRDKYTNFFLASEAGNWILKDTLELIISNIENRRIEGGVFVMTGPDTLNNAIGD
                     KEVNFRRDKVTCAQGTFTNEYFQYIDKPGSKWNYKKNEDLLK"
     gene            complement(7675..8112)
                     /gene="aroQ"
                     /locus_tag="PXA33_000675"
     CDS             complement(7675..8112)
                     /gene="aroQ"
                     /locus_tag="PXA33_000675"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HDL5120034.1"
                     /translation="MKKILLLNGPNLNMLGKREPQIYGSQTLADIENHLKKLAAAQDV
                     ELESFQANGEEPIINRIHQAFQHTDFIIINPGAFTHTSVAIRDALLSVSIPFVEVHLS
                     NVHSREPFRHHSYLSDIAKGVICGLGAKGYEYALDFAISHLKA"
     gene            complement(8198..8566)
                     /locus_tag="PXA33_000676"
     CDS             complement(8198..8566)
                     /locus_tag="PXA33_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL5120035.1"
                     /translation="MAFTIRKMVEQDTHQVFPLMQKLAVFEHYIDSFAITPEVVMESG
                     FRKSPPDFYCLVAENASNIIGILVYYFLPYTAQNRPAIYMKELYVDETARNQKVGEAL
                     MQALKNEAKAHHCKTIKSLE"
     gene            complement(8626..9450)
                     /gene="dapD"
                     /locus_tag="PXA33_000677"
     CDS             complement(8626..9450)
                     /gene="dapD"
                     /locus_tag="PXA33_000677"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HDL5120036.1"
                     /translation="MSLQAIIEAAFERRAEITPKTVDAETKAAVEEVIAGLDNGSLRV
                     AEKKDGEWVVNQWVKKAVLLSFRIADNEIIDGAETKFYDKVPTKYGNYTEEQFKADGI
                     RAVPGAVVRKGSHIEKNVVLMPSFVNIGAYVGEGTMVDTWVTVGSCAQIGKNVHLSGG
                     VGIGGVLEPLQANPTIIGDNCFIGARSEIVEGVIVEDNCVISMGVFIGQSTKIYDRET
                     GEVHYGRVPAGSVVVSGSLPSKDGSHNLYCAVIVKKVDEKTLSKVGLNDLLRSIDE"
     gene            9598..10260
                     /locus_tag="PXA33_000678"
     CDS             9598..10260
                     /locus_tag="PXA33_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDL5120037.1"
                     /translation="MENFANLLTGKTREHLVPLPNALSDKHYLQPQVVEAFLTLQHAA
                     KQAGFNLQPASTFRDFERQKGIWNAKFRGERKVHDDNGVAIDMAALSDLEKCKAMLRW
                     SAVPGASRHHWGTEIDIFDPDLLPENTPLLLEPWEYLEGGYFADLTKWLHVNAETFGF
                     YFPFDGKHSRVGFEPWHISYRPISNEYEQRFNDEILKAAWQDEEVAGKECLLEHFEEL
                     LK"
     gene            10257..11009
                     /locus_tag="PXA33_000679"
     CDS             10257..11009
                     /locus_tag="PXA33_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL5120038.1"
                     /translation="MIQLINETQQHDKFRQICDKWQLVEDKSSPLALVLTEERLELRK
                     LDEPKLGAIAVNFVDGTMAHRRKFGGGRGEAVAKAVGIKGDYLPTVIDATAGLGRDAF
                     VLAAVGCKVLLVERNPIVAALLEDGLNRAYQDVEIGSFMQERMILADVRDISQLDPQS
                     QMAEVVYLDPMYPHKQKSALVKKEMRVFQHLVGADLDSDSFFLPAKGLAKKRVVVKRP
                     DYAPFLAEQKPDFSHTTKNHRFDVYLSHLKND"
     gene            11100..11936
                     /gene="ftsN"
                     /locus_tag="PXA33_000680"
     CDS             11100..11936
                     /gene="ftsN"
                     /locus_tag="PXA33_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005613437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HDL5120039.1"
                     /translation="MPQRDYVARSNEKKSKKNQKESNVKKILMRIIGVILFFGIGLWF
                     LKSSAPVPTVPSAPQPANSQTKSELPSRPEETWSYIRDLETRTVITDNSQTSQERLAQ
                     LTAEQKQQIEERRKQEEARLAAEQAQQKPATDVSATELAPKTEEVKSLTEEELAAKKA
                     QEQALAQKQAEEKRRQAELKKQQEAKKEQPAKIVASAATLADKAANQAGKFGLQCGAF
                     KNKAQAENMQARLAMAGFNARIASSADWNRVFVGPIGDRAATSRAQSNARSVAECVIV
                     AM"
     gene            complement(11978..13159)
                     /locus_tag="PXA33_000681"
     CDS             complement(11978..13159)
                     /locus_tag="PXA33_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HDL5120040.1"
                     /translation="MKTELDMIVIGGGMIGAATALGLAKQGLRLALLEKNPLPTFNPD
                     SDYDLRISAISMGSIQLLEKLGVWEQIVAMRSTPYTQLETWEIDGFNTLFSAADLGLE
                     KLGDMVENNVIQIALWEQLKQYPNCLQAVGFEQIFANKAGEYWEVTVDGNTFAAPLLI
                     AADGANSQVRQWAGIGLTAWQYRQHCLLATVTTEKPAPSGTWQQFYPTGPRAFLPLSG
                     NHGCVVWYDSPQKIAELKMLPAEKLGVAIEQHFPSRLNAELGNIQVQASGAFPLTRQH
                     AQHYVQNGVVLVGDAAHTINPLAGQGVNLGFKDVKTLLEVIEQAVKKGKNFASEQVLQ
                     RYEQKRKPDNLLMQTGMDVFYKAFKTDLLPLKIARNVGLVLADKVSPIKKQALKYAIG
                     I"
     gene            13266..13604
                     /locus_tag="PXA33_000682"
     CDS             13266..13604
                     /locus_tag="PXA33_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5120041.1"
                     /translation="MSCKTHANHEHEHGEGCGHTAIKHNDHIDYLHDGHLHHNKHGDH
                     YDEHVLEVNETNPADCNAAHCCGDHVHGEVCGHEAVPHGDHIDYIVNGRLHHQHGDHC
                     DDHGPVEIVK"
     gene            13736..14605
                     /locus_tag="PXA33_000683"
     CDS             13736..14605
                     /locus_tag="PXA33_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HDL5120042.1"
                     /translation="MTLAIVYSRASIGVEAPLVTIEVHISNGQPKLTIVGLPEATVKE
                     AGDRVRSALLNANFIYPPQRITINLAPADLPKEGGRFDLPIAIGILAASGQIDSDLLT
                     QFEFLGELALTGHLRGVHGTIPAVISADKAKRQMILAKQNANEASLVSNATTYFAGSL
                     LEVVNMLNKRDKLPICQHISQHSAEIRPLVSRDLTDIIGQQHAKRALMIAAAGQHNLL
                     FLGPPGTGKTMLASRLADLLPEMTDEEAIETASVTSLVQNELNFQNWKQRPFRSPHHS
                     ASMVALVGGGCEN"
     gene            14697..16508
                     /locus_tag="PXA33_000684"
     CDS             14697..16508
                     /locus_tag="PXA33_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012478332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5120043.1"
                     /translation="MFYLNNDNFKREPYRGYCPSHLEKLFTKPHLDIRGNGYYYPDNE
                     YVVAWYESDFIFKSRKAQAHAKVKLVNLTCPKYTGKNSAEYLSVLYISVPLNYLHYFT
                     IGSIWKGGVAKEQFALEEFYITVEAENQDGKRENLSIVSFGESEENGLEKPFDYTIYT
                     IPTEYHNYGDDLNRLLSISYQNQKFIIHPLHIFMMHYGYSTDIKRILATYPLDEVRER
                     LFIDKVVENFDVERYVVLPKYFVKKDAIFLYHFKYDEETTGTRVKKLVSQFRLNIHNQ
                     THPIEIDFWHTQKVELKIRGVRLGNAVLCAEIIGLNQPEGEDITLVLSQSKREKSGNK
                     TEEQNNEVNNVPITRVYTREPELDELPLTDNSPDNRTVEYNKRQFELLGRQRQIRALK
                     KAREESNQKMKALTPDEIDSLGVGESDGRNGKVGLAFCFLDDTPVGKSSKLYKLWQHA
                     KAVAEWNHGEAHWYTPNLGFRNDDELLPVSLETNKCFDYPEIAIIIRLQIYGETFFLI
                     DFSMKQRDISIRGLGYKPDPNEDFLYESDMSKSELSELLSAVHHHEHLPKEYIEKKNK
                     EKTKLTTFNHSEAESSNWAYNAVQKLTSRAITKPTYF"
     gene            16517..19297
                     /locus_tag="PXA33_000685"
     CDS             16517..19297
                     /locus_tag="PXA33_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HDL5120044.1"
                     /translation="MNAEQRVIVTPTFWGKFFGKIKSVELQQNKVIVTDKKNNITEHD
                     LGKTFDFPAIQKSFFGTKLFFKDDSTEVVLSKLAKKQTDSLLLEIEKVVASNIKVKVK
                     EGFQHFANLAENQYLRDSDIPTLNDRVRLSVLSYGDNKEHFQKYFDESLVKKIQYISS
                     LFPVNAINAQTIRREYEQHVLNHRKHFFDVIESNPLTEQQRLSVIRNNDINMVLAAAG
                     TGKTSVMVAKALDLIDSGACKSEDILILAYNRDAATELSERLQERAREANLDLENSPS
                     ISTFHALGRRIIKECGKSVHLSKFAEDPMKLDRWFNKWLEDYIKEDPDNLAKFIQLAY
                     QPIDPFQFKTKDEYDRYVNDNDYRTLQGERVRGYQELLIANWLFSHSVEYEYEPRYVS
                     KRRIEIGFDYKPDFSLGDGVYLEHFGIDRQGRTRADINAQEYNANIQRKRELHAEHNT
                     TLLETYHYNWVENTLYKRLEQLMNEQFIPLKPKSQQEILDALNESGIFKENKNRYLKC
                     LQAIRTERLDYQQILKRLTDAKIVYAKEYATLLMRIHDAYVKELRSANEIDFDDMILL
                     ATQLVKTGEFKPKWKHILVDEFQDISMARLELLKEIYTKGPRPIWTVVGDDWQSIYRF
                     SGGKLEVTTRFNDMVGSHTLSKLEKTYRYNNSIADTAGQFIMQNPEQYQKNVVTHTKV
                     ADSCVHLYDSHVVKDEKSEANISLKASAILKLIRQKAPEATVAILARYRYLLEDAKSV
                     IKDKNVKFWTFHGSKGLEADYCILLGFFQGKVGFPNQNKEEAVVEALLPMLDSFPHSE
                     ERRLFYVALTRAKKESYLIADANAPSEFINELLSPKYKIDIVSKRFQERYRTIFKCNE
                     CSTGYFVMKQGKFGQFYSCTSGEVCRSSPRICEKCGRPMKLREGKFGQFWGCSGYGIK
                     GDQCTNTRKYFM"
BASE COUNT         5802 a         3916 c         4125 g         5486 t
ORIGIN      
        1 aaaagagtca tatcaatgaa tctcccttgt ctttccgcaa aaaatctgta taatttgacc
       61 gcttgttttc tcttttgaaa caagttcgtt accctcacct cgaacgaggg tttatttttt
      121 actatcaaca tttagaggaa aggttatggt aaccattcgt ttaactcgtg gcggagctaa
      181 aaaacgccca ttttaccaaa tcgtggtagt ggacagccgt tcacctcgtg atggtcgctt
      241 tatcgagcgt atcggtttct tcaacccatt agcagcaggt caagctgagc gtttacgctt
      301 agatgtggct aaagtagatg cttgggttgc taaaggtgct gatttatcag accgtgttgc
      361 ttctttagta aaagaagctc gcaaagcagc ttaattttta agccaaaatt aaataagtag
      421 gtgagtatga gcgaacaaaa gatcgaagtt gtcggaaaat taggttcaac ctatggtatc
      481 cgtggttggt tgcgtctcta ttcatcgaca gaacaaactg agagcatttt cgactaccag
      541 ccgtggtttt taaaaattaa aggtcagtgg cagcaggtcg aattagaaag ctggcgttat
      601 catagcaatg atttgattgt taaattgaaa ggcactgaag accgtgaagc cgcacagtta
      661 ttaaccaacg ctgaaattgg cgtggatttg tctgtgtttc ctgaattgga agagggagat
      721 tactattggc acgatttgat cggctgttcg gtagtaaact tagagggcta cacaatggga
      781 acagttgccg aaatgatgga aacaggttca aatgatgtgc ttgtggtgcg taataacggc
      841 aaagatgctt tcggtaaaca agaacgatta attccgtttt tgtatgaaca agtagttaaa
      901 agagtagatc tcgccaccaa aaccattacg gtggagtggg acgctggttt ctaacctcag
      961 gtatgcggtg agctttggaa aacatttaag gcgttaaaat gtggattggt attatttcac
     1021 tgtttcctga aatgtttaaa gcaattaccg atttcggggt gacaggacgt gcagttaaac
     1081 aaaatctact gcaagttgaa tgttggaatc ctcgtgattt tacattcgat aaacataaaa
     1141 ctgtagatga tcgcccttat ggcggtggtc cgggaatgtt gatgatggtg caacctttgc
     1201 gtgatgcgat tcacgcagcg aaaaaggcgg cattagcagt agaaggcggt gaagctaaag
     1261 tgatttatct ttcgccgcaa ggacgtaaac tcgatcaaaa cggtgtgcaa acactggctt
     1321 caaatcagaa actgatttta atttgtggac gatatgaagg cgtggacgag cgattgattc
     1381 aaacagaagt tgatgaagaa tggtcaatcg gtgattacgt tctcacaggg ggggaattac
     1441 ctgcaatgac attaattgat gcagttgcaa ggtttgttcc cggggtatta ggcaaacagg
     1501 cttccgcgtt agaggattct ttcgcagagg gattattaga ttgcccacat tatacccgcc
     1561 ctgaagtatt agacggtatg cctgttccgg aagtgctgat gtctggacac catgaacaaa
     1621 ttcgtaaatg gcggttggaa caatcgctgg aacgaacgtg gttaagacgc cctgagctat
     1681 tggatagcct agctctgact gacgaacagc gtgtgttgtt agctaaaatt aaaaaacagc
     1741 acaaaatcag ttaattctag gattatagaa ggtttttaaa atgagtaaca tcatcaaaca
     1801 acttgaacaa gaacagttaa aacaagactt acctagcttc cgtccgggtg acacattaga
     1861 agttaaggta tgggtagtag aaggtagtaa aaaacgtttg caagcgttcg aaggcgtggt
     1921 tattgcaatt cgtaaccgtg gcttgcactc tgcattcact ctacgtaaag tttcaaatgg
     1981 cgtaggcgtt gagcgtgtat tccaaactca ctcacctgta attgacagca tttctgttaa
     2041 acgtaaaggt gcggtacgta aagctaaact ttactactta cgtgagcgtt ctggtaaatc
     2101 tgcacgtatc aaagagcgtc ttggcgacta attgctacaa aacagtgaaa tgcacaaaag
     2161 ccttgaggaa actcaaggct tttgcttttt gtacttacaa gcagttattt ttcctcaatg
     2221 atttacaaat cttgctttct tttttgttat aatgttaaca agataacatt aatgataggt
     2281 gtcgatatga aacaattaag tattagattg ccagacgatg aatttacttt tattaaaaca
     2341 tttgcttctg ctcataaact gactttacaa aattttgtga tgtccgcaat ccaagctaaa
     2401 gttgcagaaa tgtctgctaa aaataaaata tctgaagaaa gtaacattca tcaacctctt
     2461 tctaaagatg aaaaaataat gctaaatgag gcaaaagtat tttattctgc tcgttcgcat
     2521 ttaaaggcag aaaatgtgat tcttccgagt gattttatgg agcaaattaa atcaggctgt
     2581 ggagcagaaa aaatggcaga aatgatggga tataccaaag aagattggga gtcaatgaag
     2641 taatatgggg cagattacat ttagtaatat tttcacaaaa gtgatttttg agaatgtgct
     2701 ttctgcttca actaaagaga aaataaaaca aatgcttttt cagtctgtgg ttgcattacc
     2761 tcctctccat tcagaaagaa aaatgatttt acagttgaaa aagcaaaaaa ttaccatttt
     2821 ttatcaagtt attgaggaac aatctgtgca aatcctcgct tatcaatttg gtggaactga
     2881 taaattgaca ggtgtgtcta aagctcactt taaaacacta gatgccattt ttcaaatgac
     2941 ggatgaagag aaagaaggaa aattctaatg taagcggtta tttttccata tttttttgca
     3001 aataacgttt ctcaaatttt gcttgatggc gttcaagcac ttccgtcatc gaaatgccat
     3061 atttatcggc taatacaaaa agattatcca tcacatcgcc taactcttcg attaagttat
     3121 ctcgtttttg ctgttgtgaa gcttcggttt cgtccggtcg ttcacgcccg atttcaatcg
     3181 ctcttactgc acaagccact tcgccgattt cttctgctag ataagtcacc cgcataaaag
     3241 ggcttcgttc ataccaacct tgttgttggt aaaactggcg aacccattgt tgatattgtt
     3301 caatttgcat tttatttctg attcaaaaag tagggacgaa aaatttttcg cccttttata
     3361 agaaaatgta tcagggcaaa tgatgatttg cccctaggag tgttactctg cctcagagag
     3421 aataatccag ccactatcca gccagtcgca aatggtgtct agcattagct ccagctccgc
     3481 ttggtcttgc actttggagg caagtttgtt ccacgaaatc gaatctccat tggcgatacg
     3541 aatcagtaat gccatttctg cttcgtttaa ctcatcaatc cactcgccat ttacataaac
     3601 ccgttgaggg ttctcggtgt agaggatttt tacatttgca tcttgctgta accagccgcc
     3661 ggcttcgaga atttcttgca cttcgtcagg gtaatattca ttgtcagttt gcaataattc
     3721 ataacggcgg gagcttgccg cagtggcaac actgtgagtg agtaactcat cgaactgttc
     3781 ggaattttgg agcaagtcga ttaactgatg tttaaacatt ttcaccattt tcggatcaag
     3841 caacgcattc ggctgttcat tcggcgttaa gcggaacgga agatgaaatt ccgatgaatt
     3901 aatgttgtcg gcttgttgtt cgatggtttt gcagagattt tccagtaaat cggtcgcatt
     3961 tggataacgt aaaccaaaag agaaagttaa accttgctcg ctttcggaaa caccatagtg
     4021 agccattcga gacggcacat aaagcacatc acccgggttc attacctcgt ccattaccag
     4081 ttcgcccata tcatcaaaaa tgcggatcgg ttggtttggt ttgaattcgg tgcttggatc
     4141 gcaccatttg cctaattgcc aacgacgttg cccatagcct tgcaccaaga acacatcgta
     4201 ctcatcataa tgtttgccga cagtgccgtt gagcggtgag caagaaacca taatgtcatc
     4261 acgttgccat tgcgggataa aaccaaaagc ctgccaaagc tcgcctagct caggagacca
     4321 ttgctcgagg ttttgcacca ttacagacca ttgtacaggt aactcttcca aatcttcctc
     4381 aacaagcggg ctggatttaa cagaccattt ctcgttttcg cattgaatca ggcgtgcagt
     4441 gacttcttcc tctaatgcca gttctaaaat atcttccggc tcaaacaatc cgacaatttg
     4501 gggtaagccg ttacgaatta atagcggtcg tttttgccaa tattctcgca aaaaggtttc
     4561 agggctgata ttttcaggta agcagaatgg aatgttcata atggctcctt gctacgtgct
     4621 tctttgagat tactgatatt ctacccgaga aatcaggaaa aacgggaaat tttttatgat
     4681 ttagcgacat cattcacatt ttgaagagga aataatgaaa aaggcgaaca ttgttcgcct
     4741 ttgaaattta ttttaaaccg agttttttct cgagatagtg gatattggtg ccacctttgc
     4801 ggaattcttt atccgcaaga atttgagtat gtaaaggaat attggtttta atgccttcca
     4861 caatggtttc ggaaagggca ttttccatcc ggcgaatcgc aatatcacga ttttcagcaa
     4921 aggtaatcaa tttaccaatc attgaatcgt aatgtggcgg cacgctatag cctgagtaaa
     4981 tatgtgaatc ccaacgcaca cctaaaccac ccggaacgtg taaacgggta attttacccg
     5041 gcgatggcaa gaaggtgttt ggatcttctg cgttgatacg gcactcaatg gcgtgacctt
     5101 tcactttaat atcatcttgt gtgatcgaaa gtggtaagcc ggctgccaca cgcagttgct
     5161 cttttaccaa gtccacaccg gtaatcattt cggttacagg gtgttctacc tgtaagcggg
     5221 tgttcatttc aatgaagtaa aactcgccgt tttcatataa gaactcaaaa gtacctgcac
     5281 cacggtagcc aatttcacgg caggcattgg cacaacgctc cccaataaat tgacgcaatt
     5341 caggtgtaat accctgagca ggtgcttctt ccaccacttt ttggtggcga cgttgcattg
     5401 aacagtcacg ctctgctaaa taaaccgcat taccgtgagt atcggcaagt acttggattt
     5461 caatatggcg tggattttct aaatatttct ccatatacac catatcgtta ttaaatgccg
     5521 ctttcgcttc ggctttcgtc attgcgatag attcttccaa atctttctcg tgatgaacca
     5581 cacgcatacc acgaccgcca ccaccacctg atgctttgat gatcaccggg tagccgatac
     5641 gtttagcgat ttctttattt ttcttcggat cgttaccaac cggaccgtca gaacccggta
     5701 cgcaaggcac gccggctttt ttcattgcat tgatagcaga gactttatcg cccattaagc
     5761 gaatcacatc agccgttgga ccaataaaga taaagcctga tttctcaact tgttcggcaa
     5821 agtttgcgtt ttcagataag aaaccgtagc ccggatgaat agcatcggca ttcgttactt
     5881 cggcagcggc aataatcatc gggatattta aatagctttt taccgatgga gcaggaccga
     5941 tacaaatggt ttcatcggct aatttaacgt gttttaattc acggtcggca gttgaatgca
     6001 ctgcaaccgt tttaatgccc aattctttac aagcacgcaa aatacgcagg gcaatttcac
     6061 cacggtttgc gataacaact ttttctaaca tagggaaatt ccttgtgttc tagaaaatgt
     6121 agggcgtgtt gcacacgccc gttggataat ttaaggaaga gcttattcaa tgatgaataa
     6181 tttttggtcg aattctaccg gttcgccatc attaactaag atttctttta ccacaccggc
     6241 tttgtctgac tcgatacggt tcatcatttt cattgcttct acaatacata acgtatcacc
     6301 gattttcacg gtttgaccca cttcaacgaa cggttttgca tctgggcttg ggctacggta
     6361 gaaagtcccc accattggtg aaagcactgc gtggccactc gtttctgcgg cttttggtgc
     6421 ttcagccgga gctgcaacag gtactgtcga agctacttga ggtgccgctg gtgcaacaac
     6481 aggagctgct gcatattgaa ctgcagctgg tgcaacagct ggagcagcac ggctaatacg
     6541 caccgtacct tcttcttcag acacttctaa ctcggtaatg ccagattctt caactaattc
     6601 aataagtttt ttgattttgc gaatatccat aataagattc cgtatgtgat taaataaaat
     6661 aaaaaagaga actccacatt tggggtataa attcaaatgt agagttaaaa aatgctgggc
     6721 gtagaatacc tgaatttcgc taattttgcg aataaaatct gataattttg gcgaaaatgg
     6781 acgattttag cattcatcgt accagttagg atttttggaa aatggtgact cacaagcgat
     6841 tgaaaattgc caattttttg taaaaaacaa ccgcttgtca gattatttca ataaatcttc
     6901 atttttcttg taattccact tgctaccggg cttgtcgata tactgaaaat attcgttggt
     6961 aaaagtacct tgagcacaag ttactttgtc tcggcggaaa tttacttctt tatcaccaat
     7021 ggcattattt agggtgtcag gaccggtcat aacaaacacg ccaccttcaa tccgacgatt
     7081 ctcaatattg ctgataatca gctcaagggt gtcttttaga atccaatttc cagcttccga
     7141 agccaagaaa aagttggtgt atttatcacg gcgtttaatt aacacttcgc tatcattttc
     7201 gccaataatt ttagagagtg ggtaaaccaa atgcccgtca atatcaatat aaatgccacc
     7261 ttctcggagc aaagtaaaaa ttcgccaaaa atcagcttgt gccgcaccat cggttaattt
     7321 gctataggcg ttaaaggtgc gttcatcggc gttggccttg atgtatgcct ctcgttcttc
     7381 tgtgctaacg tagcgataat cgtagctcaa tgacattagg cggttaaaca gataattcgc
     7441 atacattggc aaggtgactt tgttagaata attcgtttgc caaatggttt tggtaatttt
     7501 gctcggaatg cttgattgga tttttgccgg gctatattcc ggaatggtga aacgtttttt
     7561 cggaaaaagt gcatggaacg gataactcaa tattttgacg atattaccga ataaacggaa
     7621 gactccgtta cagatcacaa taaattgttc atttttaaca gccattttat taccttatgc
     7681 ttttaagtgt gaaattgcaa aatccaacgc atattcatag ccttttgccc ctaaaccgca
     7741 aataacgcct tttgcaatat cacttagata ggagtgatgg cgaaaaggtt cacggctatg
     7801 aacgttagac aaatgcacct caacaaacgg aatcgacaca ctcaataacg catcacgaat
     7861 tgccacgctg gtgtgagtaa atgccccggg gttaataata ataaaatcgg tatgctgaaa
     7921 ggcttggtga atacggttga taatcggctc ttcaccgttg gcttgaaagc tctccagctc
     7981 aacatcttgg gccgcagcca atttttttaa gtggttttca atatccgcca aagtttgcga
     8041 accgtaaatt tgtggctcac gtttgcccaa catatttaaa ttcgggccgt taaggagaag
     8101 gatttttttc atatttactt attcaacttg taattcataa ttaagccatt cacggttttc
     8161 tttcgccccc aagcgttgat aaaaacgaat accaccgtca ttccaaggat tttattgtct
     8221 tacaatgatg tgccttcgcc tcatttttca aggcttgcat taacgcctct cccactttct
     8281 gattccgagc agtttcatca acataaagct ctttcatata aatagcgggg cggttttggg
     8341 cagtgtaagg aagaaaataa taaaccaata tgccgataat gttagacgca ttttcagcca
     8401 ctaaacagta aaaatcgggt gggcttttgc gaaagccgga ttccattaca acttcaggag
     8461 taatcgcaaa agaatcgata tagtgctcaa acacagccag tttttgcatc agagggaaca
     8521 cttggtgggt atcttgttcg accattttgc ggattgtaaa tgccataaaa ttccttacaa
     8581 gcggtaaaaa aatccctcac ttttgcaaag cgagggaggt tttaattatt cgtcaataga
     8641 acgtaataag tcgtttaagc ccactttgct taaggttttc tcatccactt ttttcacaat
     8701 caccgcacag tagaggttgt gtgaaccatc ttttgatgga agcgagcctg aaaccacaac
     8761 agagcctgcc ggcacacggc cgtaatgtac ttcgccggtt tcacggtcgt aaattttggt
     8821 agattgaccg ataaacacgc ccattgagat cacgcagtta tcttcgacaa tcacgccttc
     8881 tacgatttca gaacgtgcac cgataaagca gttgtcgccg atgatggttg ggttcgcttg
     8941 taatggctct aacacaccac cgatgcccac gccaccagaa aggtgaacat ttttaccgat
     9001 ttgcgcacaa gaacccactg ttacccaagt gtccaccatt gtgccttcgc caacgtatgc
     9061 accgatgttc acgaaagatg gcattaacac cacgtttttc tcgatatgtg aacctttacg
     9121 caccaccgca cccggcacgg cacgaatgcc atctgctttg aattgctctt cggtgtagtt
     9181 gccgtatttg gttggcactt tgtcgtagaa tttggtttct gcaccgtcga tgatttcatt
     9241 gtcggcaata cggaatgaaa gtaataccgc ttttttcacc cattggttta ctacccactc
     9301 gccatctttt ttctcggcaa cacgcagtga accgttgtct aatcctgcga tcacctcttc
     9361 tactgctgct ttggtttcag catcaacggt tttcggagta atttccgcac ggcgttcaaa
     9421 tgccgcttca ataatagctt gtaaagacat tgttgtgttc cttcaatata taaattaaaa
     9481 cagaaaattc ggctcacttt ttaacataga aaggcgttta tttccacaag aaaaaacata
     9541 tcgcagaaat ttagtttata attccaagcc acttatttga tacaagcggt gatttttgtg
     9601 gaaaattttg caaatctgct taccggtaaa acgcgtgagc atttagtgcc gttgccgaac
     9661 gctctttcgg ataaacatta ccttcagcct caggtggttg aggctttttt gacgttgcag
     9721 catgctgcaa aacaagccgg ctttaattta cagcctgcca gtacttttcg tgattttgaa
     9781 cgccaaaaag ggatttggaa tgcgaagttt aggggtgagc gtaaggttca cgatgataac
     9841 ggggttgcga ttgatatggc ggcgttatcg gatcttgaaa agtgcaaagc aatgttgaga
     9901 tggtctgcgg tgccgggggc aagtcggcat cattggggaa cggaaattga tatttttgac
     9961 cctgatttat tgccggaaaa tacgccttta ttgcttgagc cgtgggaata tttggaaggt
    10021 ggctattttg ccgatttaac caagtggtta cacgttaatg cggaaacatt tggcttttat
    10081 ttcccgtttg acggtaaaca ttctcgagtg ggctttgagc cgtggcacat tagctatcgc
    10141 ccgatttcaa atgaatatga gcaacgcttt aacgatgaaa tcttaaaagc tgcgtggcaa
    10201 gatgaagaag tggcgggtaa ggaatgctta cttgaacatt ttgaagaatt actaaaatga
    10261 tccaattaat taacgaaacc caacaacacg acaaattccg ccaaatttgc gacaaatggc
    10321 agctggttga agataaatcc agcccgttag ccttagtgct aacggaggaa cgattggaac
    10381 tacgcaaatt agacgaaccc aagctcgggg cgattgcagt gaattttgtg gacggtacga
    10441 tggcacatcg gcgtaaattc ggtggtgggc gaggtgaagc cgttgcgaaa gcggtcggca
    10501 ttaaagggga ttatttgcca actgtgattg atgcgacagc cggtttaggg cgagatgctt
    10561 ttgtattagc agcggttggt tgtaaagtgc tgttagtgga acgcaatcca attgtcgctg
    10621 ccttgttgga agatggttta aaccgagctt atcaagatgt ggaaatcggc agttttatgc
    10681 aagaaagaat gatcttagcc gatgttcgag acatttctca gctagatcca caatcccaaa
    10741 tggctgaggt agtgtattta gatccgatgt atccgcacaa acaaaaaagt gcgttggtga
    10801 aaaaagaaat gcgggtgttt caacatttag ttggggcaga tttagattcg gacagctttt
    10861 tcttaccggc aaaaggattg gcaaaaaaac gtgtggtagt gaagcgtccg gactatgccc
    10921 cgtttttagc ggagcaaaag cctgattttt cacacaccac caaaaatcac cgttttgatg
    10981 tttatctttc tcacctgaaa aatgattaaa ggtgggtcat gcgataggca agcggttatt
    11041 tttacagtaa aatttacaaa ttatgttatc gtttatttat atttaaaaag gcggttatcg
    11101 tgccacaacg tgattatgtc gctcgttcca acgagaaaaa aagtaaaaaa aatcaaaaag
    11161 aaagtaatgt taaaaagatt ttaatgcgga ttatcggggt aattttattc ttcggtattg
    11221 gtttatggtt tttgaaatcc agtgcacctg taccaacagt tccgtcagca ccgcagcctg
    11281 ctaattcaca gactaaaagc gaattaccaa gtcgaccgga agagacgtgg agctatattc
    11341 gtgatttaga aacccgtacg gtaattaccg ataatagcca aacttcacaa gaacgtttgg
    11401 cacaattaac ggcggaacaa aagcagcaaa ttgaagaaag acgcaaacaa gaagaagcac
    11461 gcttagccgc tgagcaagct caacaaaagc ctgcgaccga tgtatctgct acagaattgg
    11521 ctccgaaaac agaggaagtt aagtcgttaa cggaagaaga attagcagca aaaaaagcac
    11581 aggagcaagc tttagcacaa aagcaagcgg aagagaaacg cagacaagca gagcttaaaa
    11641 aacagcagga agcgaaaaaa gagcagccgg ctaaaattgt agcaagtgct gcaacactgg
    11701 cagataaagc tgcaaatcag gcgggtaaat ttgggttaca gtgcggtgca tttaaaaata
    11761 aagcccaagc cgaaaatatg caagcaagat tggcaatggc aggttttaac gcccgtatcg
    11821 caagcagtgc cgattggaac cgtgtttttg tcggcccgat aggtgacaga gcggcaactt
    11881 ccagagctca atccaatgca cgcagtgtag cggaatgtgt gattgtggcg atgtaaaatt
    11941 gattgaaata ttgaagagag ctataggctc tcttttttta tatcccaatc gcatatttca
    12001 aggcttgctt tttgatgggt gacactttat cagccaatac taaaccgaca ttgcgggcaa
    12061 ttttgagcgg taataaatcg gtcttaaacg ctttataaaa cacgtccatg cctgtttgca
    12121 tcagtaaatt atccggttta cgtttttgct catagcgttg aagcacctgc tcacttgcaa
    12181 aatttttgcc ttttttgacc gcttgttcga tcacctcaag caaggtttta acatctttaa
    12241 agcctaaatt tacaccttgt ccggcaagcg gattaatggt gtgagccgca tcgccgacta
    12301 ataccacccc gttttgcaca tagtgctgag cgtgctggcg ggttagcggg aaagcccctg
    12361 aagcctgcac ctgaatgttg cctaattctg cgttgagccg gcttgggaaa tgttgctcaa
    12421 tcgcaactcc cagcttttct gctggaagca ttttcagttc ggcaattttt tgtggtgaat
    12481 cgtaccaaac aacgcagccg tgattgccgg aaagcggtaa aaaggctcga gggccggttg
    12541 gatagaattg ttgccaagtg ccgcttggag caggtttttc ggtggtaacg gtggcgagta
    12601 aacagtgttg gcgatattgc caagcggtta aaccaatgcc cgcccattgg cgaacctgtg
    12661 aatttgcacc gtctgcggca atcagcagtg gggcagcaaa ggtgttgcca tcaaccgtca
    12721 cttcccaata ctcgcccgct ttatttgcaa aaatttgctc aaatccgacc gcttgtaggc
    12781 agtttggata ttgctttaat tgctcccata acgcaatttg aataacgttg ttttcgacca
    12841 tatcgccgag cttttccaaa cccaaatcag ctgccgaaaa aagagtgttg aatccatcaa
    12901 tttcccaggt ttcgagctgg gtataagggg tgcttctcat tgcaacaatt tgctcccaca
    12961 cgcccaattt ttctaacagt tgaatcgagc ccatactaat ggcggaaatt cgcaaatcgt
    13021 agtctgaatc aggattaaag gtcggcagag ggtttttctc caacaaggct aaacgcagcc
    13081 cctgttttgc taagcctaga gctgttgctg ccccaatcat accgccaccg attacaatca
    13141 tatctaattc agttttcatt ttttcttcct ttttagttag gcaaaatact gctaaaacgc
    13201 cttgaattgt agtgcatttc agattaggat caaaccgatt tatctattta tctaaggagc
    13261 gttttatgtc ttgcaaaact catgcaaacc acgagcacga acacggcgaa ggctgtggtc
    13321 atactgctat taaacataac gaccatatcg actatttaca cgatgggcat ttacaccaca
    13381 acaaacacgg cgatcactat gacgaacacg ttttagaagt gaatgaaacc aacccggcag
    13441 actgtaatgc cgcccattgc tgcggcgacc acgttcatgg tgaggtttgc ggacacgaag
    13501 ctgtgccgca cggcgatcat atcgactaca tcgtgaatgg gcgtttgcac caccaacacg
    13561 gcgaccactg cgatgaccac gggccggttg aaattgtgaa ataagatttt tgatgggagg
    13621 ttttagcctc ccattattat ttttgcgatc tcgctcgaaa aattgaacat tcctgctacc
    13681 cctcttccga ttttcctttt atgctctcgg tattgatttc taataccgaa atgtgatgac
    13741 cttagcgatt gtttacagcc gagcctccat tggcgttgaa gccccgttgg tgacgattga
    13801 agtgcatatt agcaacggac aaccaaaatt aaccattgtc ggcttgcccg aagccacagt
    13861 gaaagaggct ggtgatcgtg tgcggagtgc gttgctgaac gctaatttta tctatccgcc
    13921 acaacggatc acgattaatc ttgctccggc agatttaccc aaagaaggcg gacgttttga
    13981 cttgccgatt gcgattggca ttctggcagc ctcagggcaa attgattctg atttattaac
    14041 gcagttcgag tttttgggcg agttggcact gacagggcat ttgcgaggag tgcacggcac
    14101 gattccggca gtgatttctg ccgataaagc caaacggcag atgatcttag ccaagcaaaa
    14161 tgcgaatgag gcatcgcttg tttccaatgc caccacctac tttgccggct cgttgttgga
    14221 ggtggtaaat atgctcaaca aacgggataa actgccgatt tgtcagcaca tttcgcaaca
    14281 ttctgccgaa attcgaccgc ttgtcagccg agatcttact gatattatcg ggcaacaaca
    14341 cgccaagcga gccttaatga ttgccgctgc cgggcaacat aatttgctgt ttctcggacc
    14401 accgggaacg ggtaaaacaa tgttggcaag ccggctggcg gatttactgc cggaaatgac
    14461 cgatgaggag gcgattgaaa ccgcctcggt gaccagcctt gtgcaaaatg agctgaattt
    14521 tcagaactgg aaacaacgcc cgtttcgttc gccccatcac agtgcatcaa tggtggcatt
    14581 agtgggaggt ggctgtgaaa actagaaata aatgtcaaaa acaagaaata aatgtcacaa
    14641 atctttctct aatttcgcta taattcgatc atcaaaactt taaggggaaa tccgttatgt
    14701 tctacctcaa taacgacaat ttcaaacgag aaccttatcg tggatattgt ccgagtcacc
    14761 ttgaaaagct ttttactaaa ccacaccttg atattagagg taacggttat tattaccctg
    14821 acaatgaata tgttgtagct tggtatgaat cggacttcat tttcaaatca agaaaagctc
    14881 aagcacatgc aaaagtaaag cttgttaatt taacttgccc caaatacact ggcaaaaaca
    14941 gtgctgaata tttaagcgtt ttgtatatct ccgttcccct taattatcta cattatttca
    15001 ctatcggcag tatctggaaa ggcggtgtcg ccaaagagca gtttgctctc gaagaatttt
    15061 acattacggt tgaagctgaa aatcaagatg gtaaacgaga aaacctctca atcgtttcat
    15121 tcggtgagag tgaggaaaac ggtttagaga aaccatttga ttacacaatc tacactatcc
    15181 ccacagaata tcacaactac ggtgatgacc taaaccgact actttccatc tcttaccaaa
    15241 atcaaaaatt cattatccat ccgctacata tttttatgat gcactacggg tactctacgg
    15301 acatcaagcg tatcttggca acatacccac ttgacgaggt aagagaacgc ctatttatag
    15361 ataaggtcgt tgagaatttt gatgtagagc gttatgtcgt tttaccgaaa tactttgtaa
    15421 aaaaagatgc gattttcctc tatcacttca aatatgacga agagactacg ggaacaaggg
    15481 taaaaaagct tgtaagccag tttaggttaa atattcacaa tcaaacacat ccaatagaaa
    15541 tagatttttg gcacacgcaa aaggttgaat tgaaaatccg tggtgttcga cttggaaatg
    15601 ctgtgttatg cgcagaaatt atcggtctta atcagcccga aggcgaagat attacgctag
    15661 tgctatctca atcgaaaaga gaaaaatcgg gtaataaaac cgaggaacaa aataacgagg
    15721 taaataacgt tcctattacc agagtttata cacgagaacc tgaattggat gagttaccac
    15781 tcaccgataa ctccccagat aatcgaacag tcgaatacaa caagcgacaa tttgaactct
    15841 tgggccgtca acgccaaatc agagcgctca agaaagcacg cgaagagagt aatcagaaaa
    15901 tgaaagcctt gactccagat gagatagact cactgggtgt aggtgaatca gatggccgaa
    15961 acggtaaggt tggactcgct ttctgtttcc ttgatgatac acctgttggc aaaagctcca
    16021 aactatacaa attatggcag cacgcgaagg ctgttgcgga atggaatcac ggtgaggcgc
    16081 actggtacac gcctaatctc ggttttagaa atgacgatga actccttccc gtttccttag
    16141 aaaccaacaa atgtttcgac taccctgaaa tcgcgataat tatccgcctc caaatatacg
    16201 gagaaacatt tttcctgatt gatttctcaa tgaagcaaag agatataagc attcgtggct
    16261 tagggtataa acccgatcca aacgaagatt ttctctatga aagtgatatg agcaaaagcg
    16321 aattatccga attattgtcc gcagttcatc atcatgaaca tctgccaaaa gagtacatag
    16381 agaaaaagaa caaagaaaaa accaaactca ctacatttaa ccatagcgaa gccgaaagta
    16441 gtaactgggc ttataacgcg gtgcaaaaat taacttcaag ggcaattact aagccgacat
    16501 atttttaatt atttttatga atgcagaaca acgtgtaatt gttactccga cattttgggg
    16561 taaatttttt ggaaaaatta aaagtgttga acttcaacaa aacaaagtta tcgtaactga
    16621 caaaaagaat aatataactg aacatgattt aggaaaaact tttgactttc cagctatcca
    16681 gaagtctttc tttggtacta aattattttt taaagacgat agcacagaag ttgttttatc
    16741 aaaacttgcg aaaaaacaaa ctgatagtct attacttgaa atcgaaaaag tagttgcatc
    16801 taatattaaa gtgaaagtaa aagagggttt tcaacacttt gcaaatttag ccgaaaatca
    16861 atatctcaga gattccgaca ttcctacatt aaatgatcgt gtaagattgt cagtgctttc
    16921 ctatggggat aacaaagaac attttcaaaa atactttgat gaaagtttgg ttaagaaaat
    16981 tcaatatatc tcttcacttt ttcctgtaaa tgccataaat gctcaaacaa ttcgaagaga
    17041 atacgagcaa catgttttaa atcatcgcaa gcatttcttt gatgtcattg aatctaatcc
    17101 gcttactgag caacaaagat tgtcagtcat tcggaacaac gacatcaata tggttcttgc
    17161 tgccgcgggg acaggaaaaa cgtctgtaat ggtcgcaaaa gcattggact taattgattc
    17221 tggtgcttgt aaaagcgaag atattttgat tcttgcatac aaccgcgatg cagcaaccga
    17281 actatccgaa cgcttacaag aacgtgcacg agaagccaat ttagatctgg aaaatagccc
    17341 tagcatctcg actttccatg cgcttggccg aagaattatt aaagaatgtg gaaaatcagt
    17401 ccacttgtca aaatttgccg aagatccgat gaaattagat agatggttca acaaatggct
    17461 agaggactac atcaaagaag atcctgataa tcttgcaaaa ttcattcaac ttgcatacca
    17521 acctatcgat ccattccaat tcaaaacgaa agacgaatac gatagatatg taaatgacaa
    17581 tgactatcga accttacaag gcgaacgcgt tcgtggttat caagagttat tgatcgcgaa
    17641 ctggcttttt agccacagcg tagaatatga gtatgaacca aggtatgtat caaaacgccg
    17701 tatcgagatt ggttttgact acaaacccga tttctctttg ggtgatggtg tttaccttga
    17761 acatttcggc attgaccgac aaggtagaac tcgcgcagat attaacgcgc aggaatataa
    17821 tgccaatatc caaagaaaac gtgagttaca cgccgaacat aatacaacat tactggagac
    17881 ataccactac aactgggtgg aaaatacgtt atataaacga ctagagcaat tgatgaatga
    17941 acagttcatt cctctgaagc caaaatcaca acaagagatt ttagatgcac tcaatgaatc
    18001 aggaatcttc aaagaaaaca aaaatcgcta tttgaagtgc cttcaagcta ttcgtacaga
    18061 gcgattagac tatcaacaaa tcttaaaacg tctaaccgat gcgaaaattg tctatgctaa
    18121 agaatatgcg acattgctca tgcgtattca tgatgcctac gttaaagaac tacgtagcgc
    18181 aaatgaaatc gactttgacg atatgatttt gttggctact caactggtga aaacaggtga
    18241 gtttaaacct aaatggaaac atattttagt cgatgaattt caagatattt ctatggctcg
    18301 attggagtta ttgaaagaaa tttatactaa agggcctcga ccgatttgga ctgttgttgg
    18361 agatgactgg caatcaatct atcgtttctc tggcggtaaa ctagaagtta ctacgcgatt
    18421 taatgatatg gtcggctcac acacgctaag taaactagaa aaaacctacc gctataacaa
    18481 tagtattgct gacacagcag gtcaattcat tatgcaaaat cctgagcagt atcagaaaaa
    18541 tgttgtaacg catacaaagg tcgctgattc ttgtgttcat ttgtatgact ctcatgtcgt
    18601 aaaggatgaa aaatcagaag ccaatatcag tttaaaagcc tcagcaatat taaaactgat
    18661 acgtcaaaaa gcacctgaag ccactgtcgc aattttggct agataccgtt accttttaga
    18721 agatgcaaaa agtgtaatca aagataagaa tgttaaattt tggacgttcc acggctcaaa
    18781 agggttggaa gcagattact gcattttact tggtttcttc caaggaaaag tcggatttcc
    18841 caaccaaaat aaagaagaag ccgttgtaga agcattgctt cctatgctag acagttttcc
    18901 acactcagaa gaacgtaggt tattttatgt ggcattaacg cgtgcgaaaa aagaaagtta
    18961 tttgatcgca gatgcgaacg cgccatcaga atttatcaat gaattattat caccgaaata
    19021 caagatagat attgtgtcaa aacgattcca agaacgttat cggacaatct ttaaatgtaa
    19081 tgaatgtagc actggctatt tcgtgatgaa acaaggtaaa tttggtcagt tctattcttg
    19141 tacatcaggg gaagtatgcc gctcttcgcc acggatttgt gaaaaatgtg gccgtcctat
    19201 gaagctgcga gaaggtaaat ttggtcagtt ttggggatgc tcaggatatg gaatcaaagg
    19261 cgatcaatgt acgaatactc gtaagtattt tatgtaatat attattttgg aggattttgt
    19321 ggatttatt
//
LOCUS       DANLRK010000026        19012 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.026, whole genome shotgun sequence.
ACCESSION   DANLRK010000026 DANLRK010000000
VERSION     DANLRK010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 19012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19012
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.026"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(180..392)
                     /locus_tag="PXA33_000686"
     CDS             complement(180..392)
                     /locus_tag="PXA33_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5120045.1"
                     /translation="MSIVIVDTDFVVHKEDEEWTTEEIKMIEEHLLEEQSAQYQVKRE
                     VPKQFYADIAERLSRGDLTAWEQLDG"
     gene            579..3164
                     /gene="leuS"
                     /locus_tag="PXA33_000687"
     CDS             579..3164
                     /gene="leuS"
                     /locus_tag="PXA33_000687"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HDL5120046.1"
                     /translation="MQQQYNPSEIEPKVQRYWAENKVFKAVKDVTKEKYYCLSMLPYP
                     SGKLHMGHVRNYTIGDVVSRYQRMIGKNVLQPMGWDAFGLPAEGAAVKNNTAPAKWTY
                     ENIEYMKGQLKMLGFSYDWDREVTTCRPEYYKWEQWFFTELYKKGLVYKKTSTVNWCP
                     NDETVLANEQVHEGCCWRCDTPVEQREIPQWFIKITDYAEELLNQLDNLPQWPDQVKT
                     MQRNWIGRSEGVEITFKIAGSNVELPVYTTRPDTFFGVSYVAIAAAHPLAELAAQNNP
                     ALAEFIREAKNTKVAEAELATMEKKGMATGLFAIHPLTGKEVPVWVANFVLMHYGTGA
                     VMAVPAHDERDFEFAQKYDLQINQVIQPLDGSEWDFSKAAYTEHGKLINSAEFDGLEF
                     DVAFTAIADKLEAMGVGKRQVNFRLRDWGVSRQRYWGAPIPMMTTEEGEVVTVPLQDL
                     PVILPEDVVMNGVQSPIKADPEWAKTTYNGKPALRETDTFDTFMESSWYYARYTCPQY
                     HEGMLDSDEANYWLPVDQYIGGIEHATMHLLYFRFFHKLLRDAGILNSDEPATKLLCQ
                     GMVLADAFYYTSPTNERIWVSPTQVTLERDEKGRIIKATDPQGRELVHSGMTKMSKSK
                     NNGIDPQEMVEKYGADTVRLFMMFASPAEMTLEWQESGVEGAKRFLGRVWNLVYSYAQ
                     NPATTALDVNALDKDQKELRRDVHKTIAKVSDDIGRRQTFNTAIAAVMELMNKLTKAP
                     LETEQDKAVMAEALSAVVRMLYPITPHICFELWQVLGNEQTIDFAPWVQADESAMVED
                     EKLVVVQVNGKVRGKITVSATATEDEVKAIAKADPNVAKFLDGQQIVKEIYVPFKMLS
                     FAVKA"
     gene            3364..3852
                     /locus_tag="PXA33_000688"
     CDS             3364..3852
                     /locus_tag="PXA33_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5120047.1"
                     /translation="MIKKLSLIAVFALTACGWHFKNNEVLPESFRTLTFESADQHSEM
                     SRILRNQLQLSDVKLVPSTANVAKLRLVSTSTDSKVVSVFKQAREAEKILTLNVDAIA
                     DIPNKGQYPLEVSVHRTFFDDSRAALAKSAEKEMIMKDMYEQASRQLIIKMAGLKKEL
                     SK"
     gene            3895..4920
                     /gene="holA"
                     /locus_tag="PXA33_000689"
     CDS             3895..4920
                     /gene="holA"
                     /locus_tag="PXA33_000689"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HDL5120048.1"
                     /translation="MQKIFPETLQFSLNKGLQPFYLLTGNDLLLVNETKDAIIHTARL
                     NGFDEKKEVEIKNDTNWEELFEAAQSMGLFFARQIMVLNFPDSPTATQFKKLAELCAF
                     IHSDLLFILHLPKFSKTMEKQAWFTQIELNLVQINCQTPELSKLPQWVSHRAKAMNLQ
                     IENEANQLLCYSYEGNLLALKQALQLLQLNATDGKITLTKAQEVVEHSAQFTPFQWID
                     ALFEGKIQRAQRILYHLQNEEVQAVVLLRIVQKELMILLEITRNPTAVVSSEQSLFIG
                     NLRTEFDRLKIWQNRRPFYNQITQRLTYQKLYALIQQLAELERKVKQEFSDDIWQELE
                     RFSAQFT"
     gene            complement(4921..5106)
                     /gene="yacG"
                     /locus_tag="PXA33_000690"
     CDS             complement(4921..5106)
                     /gene="yacG"
                     /locus_tag="PXA33_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HDL5120049.1"
                     /translation="MPETIVNCPTCEKEVVWSKESKYRPFCSERCQIIDLGDWAAEKH
                     SIASEEAELFSEDLEKY"
     gene            complement(5088..5723)
                     /gene="coaE"
                     /locus_tag="PXA33_000691"
     CDS             complement(5088..5723)
                     /gene="coaE"
                     /locus_tag="PXA33_000691"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HDL5120050.1"
                     /translation="MTYVVGLTGGIGSGKTTVSDLFSALGVEVIDADVVARQVVEPGT
                     PLLAKIVEHFGEEILTAEKALERTKLRQIVFNNEEEKTWLNNLLHPAIREEMVKKLQE
                     STACYVIWVVPLLIENKLTEYCDRILVVDVSPEIQLERATKRDNSKIETIKNIMAAQV
                     SREERLSYADDVIENNLPFEQGLLLIKEQVQALHYKYLALAKKEKECQKLL"
     gene            complement(5759..6415)
                     /locus_tag="PXA33_000692"
     CDS             complement(5759..6415)
                     /locus_tag="PXA33_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HDL5120051.1"
                     /translation="MLILFSLFIAVWLKAEIPRFAIRVNRQIYQETTSLVNIGLTEQQ
                     FLAQSVLQPKQPRSANLFFLLFPLIMLCSESPIIGTIFILLCFLSLLDMCYYLTDIRY
                     LVLIFLLVLWESLADFQHQTFTFTLAFCLLIGLVSQLMFKKEAFGSGDMLLLVALSPL
                     FRLEKMLLLILTASLLGIGYYLAYWLMKKQKLAKLAFIPFISTAMLLVLMLESRGINY
                     "
     gene            complement(6418..7614)
                     /locus_tag="PXA33_000693"
     CDS             complement(6418..7614)
                     /locus_tag="PXA33_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HDL5120052.1"
                     /translation="MTKIYEFHWQAQSRFGQKQRGKRLATNRDMLENLLLAQGYQHIR
                     ISRNFVLSANPKKEEVNSLLSQLALLVNSAMPLKQALSMLLQNCRNIRLYLWLNELIQ
                     HIESGYAFSQSLEKLGEFVTNQEIQLIKMGEQSGKLGIMLTNLAESRAKLEKLAKKVK
                     KILFYPVIVLLISLALSIGLLVLIVPQFAQLYGSKDKTLPLITDILFTLSECLIEQGT
                     VLLGTLLLAVIFYLFFAKKSRLVAKWKMALWAKMPLFSRIIEQSRIIFFSQNIALMLN
                     AHIPLDVALRSFLSEKNDDPILQQEIGTMLNLLQQGYKFSQGINPNVFGAEMAQMLEV
                     GEKSGNVAKMCEHISEMYQQKLDYQIDILSQLLEPMLMLIMGVIVGTIIVGLYLPIFD
                     MGALVE"
     gene            complement(7611..8063)
                     /locus_tag="PXA33_000694"
     CDS             complement(7611..8063)
                     /locus_tag="PXA33_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilB"
                     /protein_id="HDL5120053.1"
                     /translation="MEYSVTDLNSQRFIEISAEQWQKNCNEKQILLRYLAIPLQETEE
                     QLWLGIDDMQNLNACEIFAFIYHKQIEPVLISSDELKFLLNALSPEQSSRQSEIYEEN
                     TAYSLAHSEQVDRNDPIIQILENLFKFCLQHNASDIHFEPQKEKLLRQ"
     gene            complement(8084..8563)
                     /gene="ppdD"
                     /locus_tag="PXA33_000695"
     CDS             complement(8084..8563)
                     /gene="ppdD"
                     /locus_tag="PXA33_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HDL5120054.1"
                     /translation="MQKIVHFRPLGIRQLHRAFTLIELMIVIAIIAILATIAIPSYNS
                     YTQKAALSELLQASASYKSDVEVCIYNSGGLDNCSGGTNGIQANKTSSNTTKYLNSIA
                     VTKGVITVTGKGSLNGYGYTLTPSFSNNTISWTASCSGNDTSLFPANFCSTTVTPNG"
     gene            8744..10123
                     /gene="radA"
                     /locus_tag="PXA33_000696"
     CDS             8744..10123
                     /gene="radA"
                     /locus_tag="PXA33_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HDL5120055.1"
                     /translation="MAKAPKTAYVCNDCGAEYSRWMGQCKECKAWNTISEVRLISSKE
                     AAKSDRFSGYAGETSGKVQTLSDISLQEVPRFSSGFNELDRVLGGGVVPGSAILIGGH
                     PGAGKSTLLLQVMCGLAQHLPTLYVTGEESLQQVAMRANHLGLPTANLKMLSETSVEH
                     ICNIADQEKPKIMVIDSIQVMHLSDIQSSPGSVSQVRECASFLTRYAKTRQVAIIMVG
                     HVTKDGTLAGPKVLEHAIDASLLLEGESDSRFRTLRSQKNRFGAVNELGVFAMTEQGL
                     KEVKNPSAIFLSRSEEQTPGSSVMVLWEGTRPLLVEIQALVDHSMLANPRRVAVGLDH
                     NRLSLLLAVLHRHGGLQMSDQDVFVNVVGGVKVTETSADLALILALISSFRNRPLPQD
                     LVIFGEVGLAGEIRPVTSGQERISEAAKHGFKRAIIPHGNVPKKAIKDMQVFTVKKLS
                     DALDILYNL"
     gene            complement(10178..10408)
                     /locus_tag="PXA33_000697"
     CDS             complement(10178..10408)
                     /locus_tag="PXA33_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HDL5120056.1"
                     /translation="MDYVEILGYVAMILVACSFLLKDVIKLRVVNSLGCACFVVYGLL
                     IGSIPVTGLNALVVCINLYYISKGNRPTAAHS"
     gene            complement(10528..11022)
                     /gene="folA"
                     /locus_tag="PXA33_000698"
     CDS             complement(10528..11022)
                     /gene="folA"
                     /locus_tag="PXA33_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HDL5120057.1"
                     /translation="MKISIIVARTINKVIGKDNQMPWHLPVDLAWFRQNTLGKPVIMG
                     RKTYESIGRLLPKRPNIILSRSGFTVEGAYAVQSLEQAVELAKNFANTAEIMIIGGGE
                     LFKQALPLAERLYLTEIQADIEGDTFFEFDEQNWALVEEKWSEIDENNPFRCRFMCLE
                     RKSV"
     gene            11098..11253
                     /locus_tag="PXA33_000699"
     CDS             11098..11253
                     /locus_tag="PXA33_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl kinase"
                     /protein_id="HDL5120058.1"
                     /translation="MATLFLVTSEQIMSKTIVIKFGTSTLTHGTKSLSRPYMLELVKL
                     NYTNSIV"
     gene            11250..12218
                     /gene="proB"
                     /locus_tag="PXA33_000700"
     CDS             11250..12218
                     /gene="proB"
                     /locus_tag="PXA33_000700"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HDL5120059.1"
                     /translation="MIVVTSGAAAAGRDYLGHPELPKTLASKQMLAAVGQSQLIRVWE
                     NLFDIYNIHIGQMLLTRADLDDRERFLNARDTLDALLAQKIIPVINENDAVATEEFKV
                     GDNDNLSALVAILAQADQLYLLTDQEGLFSADPRSNPDAKLLSVVEKITPEIRQIAGG
                     SSTGLGTGGMSTKITAADIATRSGVETIIASGSRPNVLVDLANGQNIGSKFLVQSDRL
                     EGRKRWLFAAPQVGEIVVDFGAEQALLQHKSLLPVGVSAIKGEFTRGEVIKIFSQDGK
                     ALALGIARYSSEALLRIKGKKSKEIESLLGYEFGSVAVHSDEMVVY"
     gene            12218..12982
                     /locus_tag="PXA33_000701"
     CDS             12218..12982
                     /locus_tag="PXA33_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HDL5120060.1"
                     /translation="MELGIDVLAILFAVATLAGFIDAIAGGGGLLTIPALMAAGLPPA
                     MALGTNKLQACGGSLSSSLYFIRKKAVNLKQIWLLILCTFLGSAAGTIFVQMINVDFL
                     KTLLPFLILIIGIYFLVSPKIGDEDRKQRISFTLFAFTAAVALGFYDGMFGPATGSFM
                     TLAFILLLGFNLSKAVAHAKVINFTSNFASLIFFTLGGAVMWKIGLIMMVGQFIGGTL
                     GAKMVMTKGKKLIRPMLVTMSFMMVGKMLYEQGIFN"
     gene            12985..13617
                     /gene="nth"
                     /locus_tag="PXA33_000702"
     CDS             12985..13617
                     /gene="nth"
                     /locus_tag="PXA33_000702"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005721589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HDL5120061.1"
                     /translation="MNKAKRIEILTRLRNENPKPTTELNYSNPFELLIAVILSAQATD
                     KGVNKATDKLFPVANTPQAILDLGVDGLKEYIKTIGLFNSKAENIIKTCRDLIEKHNG
                     EVPEDRDALEALAGVGRKTANVVMNTAFGHPTIAVDTHIFRVSNRTNFAPGKNVVQVE
                     EKLLKVVPDEFKVDVHHWLILHGRYTCIARKPRCGSCIIEDLCEFKEKTE"
     gene            13814..15064
                     /locus_tag="PXA33_000703"
     CDS             13814..15064
                     /locus_tag="PXA33_000703"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HDL5120062.1"
                     /translation="MDIYLVGGAVRDQLLGLPVKDRDFLVVGATAEQLLAQGFQQVGA
                     DFPVFLHPETKEEYALARQERKNGNGYNGFICDFSPNVTLEQDLIRRDLTINAIAQDE
                     NSTLFDPYGGVQDLENRLLRHVSPAFSEDPLRVLRVARFAARFHSFGFTIAPETLKLM
                     REMVQSGELKHLTAERVWLETQKAFETDNPHVYFDILRLIGALKVLFPELDRLFGVPQ
                     PPQHHPEIDSGKHTLMVLQQAKRLAKKAENPTALLFAALCHDLGKGLTPADILPHHYG
                     HEVKGIQPTRELANRLKIPTEIKDFALLVTEYHTHCHKMAELRPETVIKLFNSLDVWR
                     KPNRFFDFLFACEADARGRLGFEQRNYPQPELAKKYYQAAIEIDVQQVIQDGFEKQAI
                     REELNKRRVYAVRSIKLDSDLINH"
     gene            complement(15165..16100)
                     /locus_tag="PXA33_000704"
     CDS             complement(15165..16100)
                     /locus_tag="PXA33_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HDL5120063.1"
                     /translation="MSESKPFPITTGFFAMPLGLGALGLAWLNATPITPYATTIGNII
                     AGTSVILWVALLGCYVYRVFFCFEQIKAEFMHPVQCCLFSLVTMTTMISGDVLYVWDF
                     YLGELLIFLGVIVHLIFLVFIIGGLWKGTQFLPETLHPVHYLPPIAGSFTMASSLSLI
                     GYSEIASLFLGIGIISWLVYEPTMLQRIRLSPLFPIARPSLGIVLAPAFVGASAYFSM
                     GGELDLLAKMLWGYGFLQFCFILRNLKWIAENGFSMGFWAFSFGLAAMARGAIEFYQF
                     EPLSFIGLAGFTIANFGIGLLLIFTLKLWLQQSRA"
     gene            complement(16229..16423)
                     /gene="iscX"
                     /locus_tag="PXA33_000705"
     CDS             complement(16229..16423)
                     /gene="iscX"
                     /locus_tag="PXA33_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005656371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HDL5120064.1"
                     /translation="MKWTDTRMIAENLYDMNPDLDPTTVRFTDMHKWICEMEDFDDDP
                     EASNEHILEAILTIWLEEYE"
     gene            complement(16423..16764)
                     /gene="fdx"
                     /locus_tag="PXA33_000706"
     CDS             complement(16423..16764)
                     /gene="fdx"
                     /locus_tag="PXA33_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006717450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HDL5120065.1"
                     /translation="MPKIVFLPHEEFCPEGMVVEAKAGDNLLEVAHEAGVEIHHACDC
                     SCACTTCHVVIREGFDSLNDTSDQEEDMLDKAWGLEIDSRLSCQCIVGEEDLVVEIPK
                     YNLNHANEAAH"
     gene            complement(16783..17148)
                     /locus_tag="PXA33_000707"
     CDS             complement(16783..17148)
                     /locus_tag="PXA33_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 51 family protein"
                     /protein_id="HDL5120066.1"
                     /translation="MSNYVKTEISEQFRTVAVEFDLEDNRIFELGERINTEYDVAYMN
                     GYNWAAFLRAYLSIYRPSLLELLEEDPDVGCYYVTYPLSEKSKEKARELKKIIVYLVE
                     NEEEILNFIGEHLDNIEWD"
     gene            complement(17158..19011)
                     /gene="hscA"
                     /locus_tag="PXA33_000708"
     CDS             complement(17158..19011)
                     /gene="hscA"
                     /locus_tag="PXA33_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HDL5120067.1"
                     /translation="MALLQISEPGQTAAPHQHKLAVGIDLGTTNSLVATVRSGQTQVL
                     LDEQERALVPSVVHYAETEKTVGIEAFAKAALDPKNTVISAKRLIGRSLADVQQRYQN
                     LPYQFVASENGLPLIQTAQGNKSPIEVSADILSHLAQFAEKRLAGELSGVVITVPAYF
                     DDAQRQSTKDAARLAGLNVLRLLNEPTAAAIAYGLESGQEGVIAVYDLGGGTFDLSIL
                     RLRKGVFEVLATAGDTALGGDDFDHLLAEWIAEQANYKPQTASEQRELLTLATQTKVA
                     LSQAVEFEVKFANWTGVVSRSQFNELIQPLVKRSLMTCRRALKDAGVEAEEVREVVMV
                     GGSTRVPFVREQVGEFFGKTPLTSIDPDKVVAIGAAVQADILVGNKPDSDMLLLDVVP
                     LSLGIETMGGLVEKIIPRNTTIPVARAQEFTTGKDGQTAMTIHVLQGERELVEDCRSL
                     GKITLRGIPAMAAGVAQVRVTYQVDADGLLNVTAMEKSTKVQASIQIKPSYGLTDEEV
                     TQMIKSSMANAKEDMEARQLVEQRVEADRVIDSVVTALKEDGSAVLSVEEFQAIEAQL
                     ARLIELKEGTDRFAIQQGIKEVDLATQEFAARRMNLSIQKALAGKKMDDLA"
BASE COUNT         5656 a         4171 c         3800 g         5385 t
ORIGIN      
        1 attgatagaa taccattatt tcccgttgat aaaccgctcg ctttctgaaa cgtaaatcct
       61 tgcgaaatgc gacacctgct tttggataaa actgtaaacc attgatgata ttcgtttgag
      121 tatgctccga aaataatcca tagaaaataa actctaatag attattttta aaatcaacac
      181 taaccatcta actgctccca agcagttaaa tctcctcgtg ataaacgttc agcaatatca
      241 gcataaaatt gtttcggtac ttcccttttt acttggtatt gagctgattg ttcttctaat
      301 aaatgttctt ctatcatctt gatttcttct gttgtccact cttcatcttc tttatgtaca
      361 acaaaatcgg tatctacaat cacaatagac atacctagct ccttcaagta ctggttatat
      421 attctgtatt ttgcctgttc taaattcagc tgtctagctt ttctgttact cacaatttgc
      481 aaatttttca tcaaattcca ccgcttgcca ttcaaaaaaa ggcgtttttg cggtatccta
      541 tcggcaattt tttcagttaa atttaaaggt taaattttat gcaacagcag tataacccaa
      601 gtgagattga acctaaggtt cagcgttatt gggcagaaaa taaagttttt aaagcagtta
      661 aagacgttac caaagagaaa tactattgcc tttcaatgtt gccttatccg tcaggtaagc
      721 tacatatggg gcacgttcgt aactacacca ttggtgatgt agtgtctcgt tatcagcgta
      781 tgatcggtaa aaatgtatta cagccgatgg gttgggacgc tttcggttta ccggcggaag
      841 gtgctgcggt gaaaaataat accgcaccgg caaaatggac ttacgaaaac atcgaatata
      901 tgaaaggtca gcttaaaatg ttgggcttct cttatgactg ggatcgtgaa gtcaccactt
      961 gccgtccgga atactacaaa tgggagcaat ggttcttcac agaactttat aaaaaaggct
     1021 tagtgtataa aaaaacctca accgtaaact ggtgtccgaa tgatgaaact gtgcttgcca
     1081 acgagcaagt tcacgaaggt tgttgctggc gttgtgacac cccagtggaa cagcgtgaaa
     1141 ttccacaatg gtttatcaaa atcactgact atgctgaaga attactcaat cagttagata
     1201 atctcccaca gtggccggat caagtaaaaa ccatgcaacg caactggatc ggtcgttccg
     1261 aaggggtaga aatcaccttt aaaattgccg gttcaaacgt agaattaccg gtttacacta
     1321 cccgtccgga taccttcttc ggcgtaagct atgtggcgat tgctgcggct cacccattag
     1381 cagaattagc tgcacaaaat aacccggcat tggctgaatt tatccgtgaa gcgaaaaaca
     1441 ccaaagtggc agaagcagaa ttagccacca tggagaaaaa aggaatggca accggcttgt
     1501 tcgccattca cccgttaaca ggaaaagaag taccggtttg ggttgctaac tttgtgttaa
     1561 tgcactacgg tacaggtgcg gtaatggcgg ttccggctca cgatgagcgt gactttgaat
     1621 ttgcacaaaa atacgatttg caaattaatc aggtaattca accgcttgat ggctcagagt
     1681 gggatttcag caaagccgct tacaccgaac acggtaaatt aatcaactct gccgagtttg
     1741 acggcttgga atttgatgtg gctttcactg cgattgccga taaattagag gcaatgggtg
     1801 taggtaaacg ccaagtgaat ttccgtctgc gtgactgggg tgtttctcgt caacgttatt
     1861 ggggtgcacc aatcccaatg atgaccacag aagagggcga agtggttacc gttccattac
     1921 aagatttacc ggtgattctc cctgaagatg tggtaatgaa cggcgtgcaa agcccaatca
     1981 aagccgaccc agagtgggcg aaaaccactt acaacggcaa accggcattg cgtgaaaccg
     2041 ataccttcga tacctttatg gagtcctctt ggtattatgc acgttatact tgcccacaat
     2101 atcacgaagg aatgttagat tcagacgagg caaactactg gctaccggtc gatcaatata
     2161 tcggcggtat tgaacacgcc acaatgcact tgctctactt ccgtttcttc cataaattgt
     2221 tgcgtgatgc gggtatttta aattcggacg aaccggcgac taaactgtta tgccaaggta
     2281 tggtgcttgc cgatgcgttc tactacacca gcccaactaa cgagcgtatt tgggtcagcc
     2341 cgacacaagt caccttagaa cgtgatgaga aaggtcgtat catcaaagca accgatccac
     2401 aagggcgtga gctggttcac agcggtatga ctaaaatgtc gaaatcaaag aacaacggta
     2461 tcgatccgca agaaatggtg gaaaaatatg gtgctgacac agttcgcttg tttatgatgt
     2521 ttgcctcccc tgcggaaatg acattagaat ggcaagaatc cggcgttgaa ggggcaaaac
     2581 gtttcttagg tcgtgtttgg aatttagttt atagctatgc ccaaaatccg gcaacgactg
     2641 cattagatgt gaatgcctta gacaaggatc aaaaagaact tcgccgtgat gtacacaaaa
     2701 cgattgctaa ggtgagtgat gatattggtc gtcgtcaaac cttcaataca gcgattgctg
     2761 cggtgatgga attgatgaac aagctcacca aagccccact tgaaaccgaa caagataaag
     2821 ccgtaatggc agaagcttta agtgcggtgg tgcgtatgct ttacccaatc acgccacata
     2881 tctgctttga gttatggcaa gtgttaggta atgaacaaac catcgatttt gccccttggg
     2941 tacaagccga tgaatccgca atggtggaag atgaaaaatt agtggtagta caagtcaacg
     3001 gaaaagtgcg tggcaaaatc actgtttcag ctactgctac ggaagatgaa gttaaagcta
     3061 tcgctaaagc cgatccaaac gttgctaaat tcttagatgg tcagcagatt gtaaaagaaa
     3121 tctacgttcc atttaaaatg ttgagctttg cggttaaggc gtaacaaaca cactctccct
     3181 tatatgggag agagaccgat aaatatttcg tgcagaaata tttatcagag agagggtaca
     3241 agcggttatt ttcttggaaa attttgcaaa aaatagcccc ctctccctgc tttttcttct
     3301 aaacgaagaa aaacctgtcc ctctcccaca aggggagagg ggaataaaca aggaaatctc
     3361 attatgatta aaaaactctc tctcatcgct gtttttgctc ttaccgcctg cggttggcac
     3421 tttaaaaaca atgaggtatt acctgaatcg ttcagaacat taacctttga aagtgcagat
     3481 caacacagcg aaatgtctcg aattttacgc aatcagttac aactaagcga tgtaaaatta
     3541 gtgcctagca cggctaatgt ggcaaaatta cgtttggttt caacctcaac agacagcaaa
     3601 gtggtttctg tgtttaaaca agctcgtgaa gcagaaaaaa tcttaacgct aaacgttgat
     3661 gcgattgccg atattccaaa taaagggcaa taccctttag aagtttcggt tcatcgtact
     3721 ttctttgatg actctcgtgc agctttggca aagtctgctg aaaaagagat gattatgaaa
     3781 gatatgtatg aacaagcctc tcgccaatta attatcaaaa tggcgggctt aaaaaaagaa
     3841 ctctcaaaat aatcattgta ggggcgaaat atgtttcgcc cattgtttat ctgtatgcaa
     3901 aaaatctttc cggaaacact tcaattttca ttaaacaaag gactacagcc cttttatcta
     3961 ttaacaggga atgatctgct attggtgaat gaaaccaaag atgccattat tcacaccgcc
     4021 cgtctcaacg gttttgatga aaaaaaagaa gttgaaatca aaaacgatac taactgggaa
     4081 gaacttttcg aagcagctca atcaatggga ttgttttttg cacgccaaat tatggtgctg
     4141 aattttccgg attcccccac agcgacacag tttaaaaaac tggctgagct ttgtgccttt
     4201 attcatagtg atttgctctt tatcctgcac ttacctaaat tttccaaaac aatggaaaaa
     4261 caggcttggt tcacgcaaat tgagctaaat ctcgtgcaaa ttaactgcca aacaccggaa
     4321 cttagcaaac ttccacaatg ggtcagtcat cgagcaaaag cgatgaattt gcaaattgaa
     4381 aatgaagcaa accaactcct ttgttatagc tacgaaggca atctactggc gcttaaacag
     4441 gcattacaac tgctacaact caatgcaact gatggaaaaa tcacgctgac aaaagcccaa
     4501 gaagtggttg aacactccgc ccaatttact ccatttcaat ggattgatgc attatttgaa
     4561 ggaaaaatcc aaagagctca acgcattttg taccatctgc aaaatgaaga agtacaagcg
     4621 gtcgttttat taagaattgt gcaaaaagag ttgatgattt tactcgaaat cactcgcaac
     4681 ccaaccgcag tcgtttccag cgaacaatca ctttttatcg gtaatttacg aacagaattt
     4741 gaccgcttga aaatttggca aaaccgcaga cctttttaca accagatcac tcaacgactg
     4801 acttaccaaa aactttacgc cctaatccaa caactggctg aattagagcg taaggtgaaa
     4861 caagaattta gcgatgatat ttggcaagaa ttagagcgtt tttccgctca atttacctaa
     4921 ctaatacttt tctaaatctt cgctaaacaa ctccgcttct tcactggcga tagagtgctt
     4981 ttctgccgcc caatcgccta aatcaataat ttgacaacgc tcgctacaaa acgggcggta
     5041 tttactttct ttactccaca ccacttcttt ttcacaagtc gggcagttta caatagtttc
     5101 tggcattctt tttccttttt tgctaatgct aagtatttat aatgtaaggc ttgaacctgc
     5161 tctttaatta aaagcaagcc ctgttcaaat ggcaagttat tttcaatcac atcatcagca
     5221 taacttaatc tttcttcacg gctgacttgg gctgccataa tatttttaat ggtttcaatt
     5281 ttactgttat cacgtttggt tgctctttcc agttggattt caggagaaac atcaacaacc
     5341 aatatacgat cacaatattc ggtgagctta ttttcaatta aaagcggcac gacccaaatc
     5401 acataacaag cggtcgattc ttgcaatttt tttaccattt cttctcgaat ggcggggtga
     5461 agtaaattat tgagccacgt tttttcctct tcattgttaa acacaatttg gcgaagttta
     5521 gtgcgttcta atgctttttc agcggttaag atctcttcac caaaatgctc tacaatcttg
     5581 gctaaaagcg gtgtacccgg ttcaacaact tggcgagcca ccacatcagc atcaattacc
     5641 tctaccccaa gagctgaaaa taaatcagac actgtggttt tgccactgcc tattccaccg
     5701 gttaagccta caacgtaagt cataaaaatt gattcctcaa aataaaaaca aaccaattct
     5761 agtaattaat ccctctgctt tcaagcatta gcaccagcaa catcgctgtg ctaataaacg
     5821 gaataaacgc caattttgct aatttctgct ttttcattag ccaataagcc aagtaataac
     5881 caatccctag caggctggcg gttaatatca ataagagcat tttttcgagc cgaaagagtg
     5941 gggataacgc aaccaataac aacatatcgc ccgagccaaa tgcctccttt ttaaacataa
     6001 gctgtgaaac caagccaatc aacaggcaaa aagcaagggt aaaggtaaaa gtttggtgtt
     6061 ggaaatcggc taaactctcc cataacacca acaaaaatat aagcacgagg tagcgaatat
     6121 ccgttaggta atagcacata tccagcagtg ataaaaaaca gagcagaata aaaatcgtgc
     6181 cgataatagg gctttccgag cagagcataa tcaatggaaa aaggagaaaa aatagatttg
     6241 ccgatcgggg ttgtttcggt tgtagcaccg attgagccaa aaactgttgc tcggttaagc
     6301 caatattcac taatgaagtt gtttcttggt aaatttggcg attgactcga atggcaaatc
     6361 gagggatctc cgctttgagc caaaccgcaa tgaagaggga aaacagtatt aacatcgcta
     6421 ctccaccaaa gcccccatat caaaaatcgg taaatacagc ccaacaataa tagtgccgac
     6481 aatcacgccc attatcaaca ttagcatcgg ttctaatagc tgagacaaaa tatcaatttg
     6541 gtaatctaat ttttgttggt acatctcact aatatgctca cacattttcg ccacattgcc
     6601 acttttctcc ccaacctcaa gcatttgtgc catctcagca ccaaacacat tcgggttgat
     6661 cccttgtgaa aatttgtagc cttgttgtaa caaattaagc atagtgccaa tttcttgctg
     6721 taaaatcggg tcatcatttt tctcggataa aaacgacctc aaggcaacat caagcggaat
     6781 atgagcattc agcattaagg caatattttg gctgaaaaag ataatgcgag attgctcaat
     6841 aattcggcta aacagtggca ttttagccca tagagccatt ttccactttg cgactaacct
     6901 gctctttttt gcaaaaaaca ggtaaaaaat gaccgctagt agtaacgtgc caagtaatac
     6961 tgtgccttgc tcaatcaagc attcggaaag ggtaaataaa atatcggtaa tgaggggtaa
     7021 agttttgtct tttgagccat aaagttgggc aaactgcggt acaatcaaaa ccaatagccc
     7081 gatagataaa gcgagcgaaa tcagcaacac aatcaccggg taaaacagga ttttcttcac
     7141 ttttttcgcc aatttttcca atttcgctcg gctttcagct aaattagtca gcataatgcc
     7201 cagttttccg ctttgttcgc ccattttgat tagctgaatt tcctgattgg tcacaaattc
     7261 acccaatttc tccaacgatt gtgaaaaagc atagccactt tcaatatgct gaatcagctc
     7321 attaagccat agataaaggc ggatatttcg gcagttttgc aacaacatag ataatgcctg
     7381 ttttaacggc atggcagaat taaccaacaa tgcaagctgt gatagcaagc tattcacctc
     7441 ttcttttttc gggtttgctg aaagcacaaa atttcggcta atccgaatat gctgataacc
     7501 ttgtgccagc aaaaggtttt ccaacatgtc tcgattcgtt gccaaccgct tgccccgctg
     7561 tttttgccca aatcggctct gggcttgcca atgaaattca tagattttcg tcattgccta
     7621 agcaatttct ctttttgtgg ttcaaaatga atatctgagg cgttgtgttg caaacagaac
     7681 ttaaataaat tctctaaaat ttggataatc ggatcgtttc tatccacttg ttccgaatga
     7741 gccaatgaat acgccgtatt ctcctcataa atctcactct gccgactact ctgctcaggc
     7801 gaaagggcat tgagtaaaaa cttcaattca tcggaggaaa tcaaaacagg ctcaatctgt
     7861 ttgtggtaga taaacgcaaa aatctcacag gcattgagat tttgcatatc atcaatccct
     7921 agccaaagct gttcttctgt ttcttgcagc ggaatggcta aatatcgcaa taaaatctgc
     7981 ttttcattgc aatttttctg ccactgctct gctgaaatct cgatgaacct ttggctgttt
     8041 aaatccgtta ccgaatactc cattttgatt tcctcacctc aaattagccg tttggtgtaa
     8101 cagttgtgct acagaaatta gctggaaata ggctcgtatc attgccacta caacttgccg
     8161 tccaactaat cgtattattt gagaacgacg gtgttaaggt atagccataa ccattcagag
     8221 agcctttgcc ggttaccgta atcacgcctt ttgttaccgc aattgagttt aggtatttag
     8281 tggtattact actggtttta ttcgcctgaa tcccattcgt gccaccggag cagttatcca
     8341 acccgccact gttgtaaata cagacttcaa cgtctgattt atacgaagca gatgcttgta
     8401 ataactccga taacgccgcc ttttgggtgt atgagttata ggaagggatc gcaatagtgg
     8461 caagaatggc aataatcgca atcacgatca tcaactcaat gagggtaaat gcccgatgca
     8521 gttggcgaat gccaagcggt ctaaaatgta caattttttg catataaatt ccttataaat
     8581 tgcaccaaca aattgtggcg aatcaagtgt gcaaattttt tgcctgcttt gcgaccggca
     8641 aattttaatt tttcgacgca gatcgaaaaa ataaaatttt tatctgttta tccatacagt
     8701 tttttgttag aatttgccaa atttactcaa ttaggaatca agaatggcaa aagcacctaa
     8761 aaccgcttat gtttgtaacg attgcggggc tgaatattcc cgttggatgg ggcaatgtaa
     8821 ggaatgtaag gcttggaata ccatcagtga agtgcggttg atttccagca aagaagcggc
     8881 gaaatctgac cgctttagcg gctatgccgg tgaaacgtca ggcaaagtac aaacgctgtc
     8941 tgacatcagc ttgcaggaag tgcctcgctt tagcagtggc tttaacgagc tggatcgtgt
     9001 actcggtggc ggcgtagtgc cggggtcagc aattctgatc ggcggtcacc cgggggcggg
     9061 gaaaagtacg ttattactgc aagtaatgtg cggtttagcc caacatctgc caacgctgta
     9121 tgtcaccggt gaagagtcgt tacaacaagt tgcaatgcgg gcaaatcact taggcttacc
     9181 aacggcaaat ctgaaaatgt tgtctgaaac ctctgtcgag catatttgca atattgcaga
     9241 tcaagaaaag cccaaaatta tggtgattga ctcgattcag gtgatgcact tgtcggatat
     9301 tcagtcctca cccggcagtg tttcacaagt gcgtgaatgt gcatctttcc ttacccgcta
     9361 cgccaaaacc cgccaagtgg cgattattat ggttgggcac gtcactaaag atggcacgct
     9421 tgccggcccg aaagtgttgg aacacgccat cgatgcttcc ttgctgttag aaggcgaatc
     9481 cgattcccgt ttccgtactt tgcgtagcca gaaaaatcgt tttggtgccg tcaatgaatt
     9541 gggcgtattt gcgatgaccg aacaagggct aaaagaggtt aaaaacccat ctgcgatttt
     9601 tttaagccgt agcgaagagc aaacgccggg cagttcggtg atggtgttat gggaaggaac
     9661 acgcccattg ttggttgaaa ttcaagcctt agtcgatcac tcaatgctgg caaaccctcg
     9721 ccgtgtggcg gttggcttag atcataaccg tttatcgcta ttacttgcgg tgctacaccg
     9781 acacggtggc ttgcagatgt cggatcaaga tgtgtttgta aatgtggtcg gtggggtgaa
     9841 agtcacggaa accagtgccg atttagcctt aattttggcg ttaatttcca gcttccgtaa
     9901 ccgtccattg ccgcaagatt tggtgatttt tggggaagtc ggcttagcag gggaaatccg
     9961 cccggttacc agtgggcaag agcggatcag cgaagccgca aaacacggct ttaaacgagc
    10021 gataattcct cacggcaatg tcccgaaaaa ggcaattaaa gatatgcagg tgttcaccgt
    10081 gaaaaaactc agtgatgcgt tagatattct gtataatctc taaaatcaag aaccgcttgt
    10141 aagtctgtct tacaagcggt ctgttttagg taaaaattta cgaatgtgcg gcagttggtc
    10201 tattgccttt ggaaatatag tataaattaa tacacaccac caacgcattt aagccggtga
    10261 ccggaatcga gccaatcagc aaaccataaa caacgaaaca ggcacaaccc agcgaattca
    10321 ccacccgcag tttaatcaca tcttttaata aaaaagaaca ggcaactaaa atcatcgcca
    10381 catagccgag tatttcaaca tagtccattt agaacctcct caaataaaat aaactgccga
    10441 tattttaaag gctgtttttt tatttgtatc tagctctaaa aatagcaaag gtttgcgttc
    10501 acatttttac ttttaaccgc ttagttatta aacagatttt cgttctaaac acataaaacg
    10561 gcaacggaaa gggttatttt catcaatttc tgaccacttt tcttctacta acgcccaatt
    10621 ttgttcatca aattcaaaga acgtatcccc ttcaatgtct gcttgaatct cagttaaata
    10681 aagtctttct gctagtggta aggcttgctt aaacagttcc cccccaccaa taatcattat
    10741 ttcagctgta tttgcaaaat ttttcgctaa ttcaaccgct tgttcgagcg attgtaccgc
    10801 ataagcccct tccaccgtaa aacctgaacg ggaaagaata atattagggc gttttggcaa
    10861 taacctgccg atagattcat aggttttccg ccccataatc accggtttgc cgagcgtgtt
    10921 ttggcgaaac caggctaaat ctaccggcaa atgccacggc atttggttat ctttgccaat
    10981 caccttgttg attgttcggg caacaataat gctgattttc attttttctc ctgttatgca
    11041 aattggtttg attttatatc aaattaagcg tgattttaca ccctaaaagt gatacttttg
    11101 gcaacattat ttttagttac aagtgaacag attatgtcaa aaactatcgt gataaaattt
    11161 ggcaccagca ctctcacaca cggcactaaa agtttaagcc gtccttatat gttagagctg
    11221 gtaaagctca attacaccaa cagcatcgtg tgattgtggt gacatccgga gctgccgctg
    11281 ccggtcggga ctatttagga caccctgaac tccctaaaac cttagcctct aagcaaatgt
    11341 tagcagcggt cgggcaaagt cagctcattc gagtgtggga aaatttattc gatatttata
    11401 atatccatat cgggcagatg ctcttaaccc gagccgatct ggacgatcgt gaacgtttcc
    11461 tcaatgcccg tgataccctt gatgccctgc ttgcacaaaa aatcattccg gtgattaatg
    11521 aaaacgatgc cgttgctacc gaagaattta aagtaggcga taacgataat ttatcggctt
    11581 tagttgcgat tttggctcaa gcagatcagc tctatttatt aaccgaccaa gaaggcttat
    11641 tcagtgccga tcctcgctca aaccctgatg ctaaattgct ctcggtggtg gaaaaaatca
    11701 cgcctgaaat ccgccaaatt gccggtggta gttcaaccgg tttaggcacg ggcggaatga
    11761 gtaccaaaat tactgctgct gatattgcta cccgctccgg tgtggaaacc atcatcgcct
    11821 ccggttctcg cccaaatgtg ttagttgatt tagcaaacgg gcaaaacatc ggcagtaaat
    11881 ttttagtcca atctgaccgc ttggaaggca gaaaacgctg gttatttgct gctccgcaag
    11941 tgggtgaaat tgtggtagat ttcggggcgg aacaagcctt gcttcaacat aaatcattac
    12001 tgccggtggg agtttctgcc atcaaaggcg aatttactcg aggtgaagta atcaaaattt
    12061 tcagccaaga cggcaaagcc ttagcactcg gcattgcccg ttatagcagt gaggcattgc
    12121 tgcgaattaa aggtaagaaa tcaaaagaaa ttgaatcgct ccttggttat gaatttggtt
    12181 ctgttgcagt gcatagtgac gaaatggtgg tgtattaatg gaattgggaa ttgatgtttt
    12241 agcaatcttg tttgccgttg cgacccttgc cggctttatc gatgccatcg ctggcggcgg
    12301 tggcttactc actattcctg cattaatggc agcagggctt cctccggcaa tggcacttgg
    12361 cacgaataaa ttacaggcgt gtggcggatc actctcttcc tcactctatt ttattcgcaa
    12421 aaaagctgtt aatttaaaac aaatttggtt gctaattcta tgcacttttt taggctctgc
    12481 cgccggtact atttttgtgc aaatgatcaa tgtcgatttc ttaaaaacat tgttgccttt
    12541 cttaattctg attatcggca tctatttttt ggtcagccct aaaattggcg atgaagatcg
    12601 taaacaacgc attagcttta ccctatttgc ctttaccgcc gctgtcgctc tcggttttta
    12661 tgatggaatg ttcggaccgg caaccggctc ttttatgact ctggcattta ttttgctgct
    12721 cggcttcaac ctctctaaag ccgtagcaca cgccaaagtg attaatttta cctccaactt
    12781 tgcctcatta attttcttta cgcttggtgg tgcggtaatg tggaaaatcg gcttaattat
    12841 gatggtcggg caatttattg gcggcacact aggtgcgaaa atggtgatga caaaaggcaa
    12901 aaaattaatc cgtccgatgc tagtgactat gtcttttatg atggtcggca aaatgctcta
    12961 cgagcaagga atttttaatt aacaatgaat aaagcaaaac gtattgagat tttaacccgt
    13021 ttacgcaatg aaaatccaaa accgacaact gaactcaatt acagtaatcc gtttgaatta
    13081 ttaattgccg tgattctatc cgcccaagcg accgataaag gcgtaaataa agccaccgat
    13141 aagctcttcc ctgtcgccaa cacgccacaa gcgattttag atttaggcgt agatgggcta
    13201 aaagagtaca tcaaaactat cggcttattt aacagcaagg cagaaaatat tatcaagacc
    13261 tgccgagatc tgatcgaaaa acataacggc gaagtgcctg aagacagaga tgccctcgaa
    13321 gcccttgccg gtgttggcag aaaaaccgcc aatgtggtga tgaataccgc attcggacac
    13381 cctacgattg cagtcgatac ccatattttc agggtgtcga accgcacgaa cttcgctccc
    13441 ggcaaaaatg tagttcaggt agaagaaaaa ctgctcaaag ttgtgccgga cgaatttaaa
    13501 gtcgatgttc accattggtt gattttacac ggacgctaca cttgcattgc ccgtaagcct
    13561 cgttgtggtt cttgtattat tgaagattta tgtgagttta aagagaagac ggaataacat
    13621 tatctggtgg tatttaatga ataactcccc tctttagaaa agtaggggct gggggagatt
    13681 tgatactatt tcaacatttc gtatgagcgt atagaaattc tgccaaatct tccctatccc
    13741 ctctttgcta aagaggggaa ctagatcgag cgtaaaatat aacaagcggt catttttttg
    13801 agattttttg caaatggata tttatttagt cggtggagca gtgcgggatc aacttcttgg
    13861 gctgccagtg aaagatagag attttttagt ggttggcgca actgccgaac aacttttagc
    13921 ccaaggattt caacaagttg gggcagattt tccggtgttt ttacaccctg aaaccaaaga
    13981 agaatatgcc cttgctcgcc aagaacgcaa gaacggcaat ggttacaacg gctttatttg
    14041 cgatttctcc ccaaacgtca cgcttgagca agatttgatc cgccgagatt taaccattaa
    14101 cgccatcgcc caagatgaaa acagcacact ttttgatcct tatggcggtg tgcaagattt
    14161 agaaaatcgg ctactccgcc acgtttctcc tgcattcagc gaagatccgc tacgggtgct
    14221 acgggttgcc cgctttgccg cccgttttca ttcatttggt tttaccattg ctcctgaaac
    14281 cctgaaatta atgcgagaga tggtacaaag cggtgaattg aaacatttaa ccgctgaacg
    14341 tgtgtggttg gaaacacaga aagcctttga aaccgataat ccgcacgttt attttgacat
    14401 tttacgctta attggggcat taaaagtgct attccctgaa ctggatcgtc tgtttggcgt
    14461 accacaacca cctcaacacc accctgaaat tgactccggc aaacacactc taatggtgct
    14521 acaacaggca aaaagacttg caaaaaaagc ggaaaatccg accgctttac tctttgcagc
    14581 cctttgccac gacttaggca aaggcttaac gcctgctgat attttgcctc atcactacgg
    14641 acacgaagtc aaaggcattc aaccaacccg agagttagcc aatcggttaa aaatcccaac
    14701 tgagattaaa gattttgccc ttttagtgac cgaataccac acgcattgcc ataaaatggc
    14761 ggaacttcgc cctgaaacgg taataaaact gtttaacagc ctagatgtgt ggcgtaaacc
    14821 aaaccgcttt tttgatttcc tgttcgcctg tgaagcggat gctagagggc gattaggttt
    14881 tgaacagcgg aattaccccc aacctgaatt ggcgaaaaaa tattaccaag ccgcaatcga
    14941 aattgatgtg cagcaagtta ttcaagacgg ttttgaaaaa caagcaattc gggaagagct
    15001 gaataaacgc agagtgtatg cggtaagaag catcaagcta gatagcgacc taataaacca
    15061 ttaggttatc cgccactttc tcaaaatcaa ttcccaaagt tcaagttggt taaatgtatg
    15121 gattctttaa aaggtggcgt tattttagtg aatttataat gttcctatgc tcttgattgt
    15181 tgtagccaca atttcagggt aaagatcagc aataacccaa tgccgaaatt ggcaatagtg
    15241 aagccggcta acccaataaa agataaaggt tcaaattgat aaaattcaat cgcacctctt
    15301 gccattgcag ctaaaccaaa tgaaaatgcc cagaaaccca tcgagaagcc attttcagct
    15361 atccatttga ggtttcgtaa aataaaacag aattgtaaga atccatatcc ccataacatt
    15421 tttgccaaca agtctaattc tccacccata gaaaaatagg cagaagcgcc cacaaatgcc
    15481 ggagcaagaa caattcctaa agatggtcta gcaatcggaa aaagcggact aagccgaatt
    15541 ctttgtaaca tagtgggttc atatactaac caggaaatta tgccaatgcc taaaaataaa
    15601 gatgcaattt cactgtagcc aattaagctt aatgaacttg ccatagtgaa gctccctgca
    15661 attggcggta ggtaatgtac cgggtgtagc gtttccggca aaaattgtgt gcctttccat
    15721 aatccaccaa taataaaaac caagaaaatt aaatgcacaa tgacgcctaa aaaaatcaat
    15781 agttcgccta aataaaaatc ccatacatag agcacatcac cgcttatcat ggttgtcatt
    15841 gtcactaagg aaaataaaca acattgcact gggtgcataa attctgcttt aatctgctca
    15901 aaacaaaaaa acacacgata cacataacag cctaataagg ctacccaaag tatgactgac
    15961 gttccagcaa tgatattacc aattgttgta gcataaggag tgataggcgt tgcatttaac
    16021 caggcaaggc ctaacgcccc cagccctaat ggcattgcaa aaaagcctgt agttattgga
    16081 aaaggtttag attcactcat aaatttatcc tcatattgaa ataatgcata actgtattcg
    16141 gctttataac agtcatatgt aaaaaatttt atttttctaa ccgcttgtaa taagaaaaac
    16201 ctaaagcact tgctttaggt tcctactact attcatactc ttctagccag atagttaaaa
    16261 tcgcttcaag aatatgttca tttgatgctt ccggatcatc atcgaaatct tccatttcac
    16321 aaatccattt atgcatatct gtaaaacgaa ccgtggtcgg gtctaaatcc gggttcatat
    16381 catacagatt ttcagcgatc attcgggtat ctgtccattt cattagtgcg ctgcctcatt
    16441 tgcgtgattt aaattatatt ttggaatttc aacaaccaaa tcttcttcac caacaataca
    16501 ttggcaactt aaacggctgt ctatttccaa tccccaagct ttatccagca tatcttcttc
    16561 ttgatctgaa gtatcattta acgaatcaaa accctcacga attaccacgt ggcaagtggt
    16621 acaagcacaa gaacagtcgc aagcgtgatg aatttcaacg cctgcttcgt gtgctacttc
    16681 taataaatta tctccggctt tagcttcaac aaccatacct tccgggcaaa attcttcgtg
    16741 tggaagaaaa acaatttttg gcattttaaa gtttccttat tactaatccc attcaatatt
    16801 atccagatgt tcgccgatga agtttaaaat ctcttcctca ttttcaacca gataaacaat
    16861 aatcttcttt aattcccttg ctttctcttt acttttctcg gaaagcggat aggtaacata
    16921 ataacatcct acgtccggat cttcttctaa taactctaat aggcttggac gataaatact
    16981 aagataagcc cgtaaaaatg cagcccagtt ataaccgttc atataggcaa catcatattc
    17041 agtattaatc cgctcgccca actcaaaaat gcggttatcc tctaaatcga attcaaccgc
    17101 aacggttctg aattgttctg aaatctcggt ttttacatag ttagacatac tgcctcctta
    17161 tgctaaatcg tccatttttt taccggctaa tgctttttga attgataaat tcatacgccg
    17221 tgctgcaaat tcttgcgtag ctaaatccac ctctttgata ccttgctgaa ttgcaaagcg
    17281 atcagtacct tcttttaatt cgattaaacg ggctaattgt gcttcaatcg cttggaactc
    17341 ctccacactc aacactgcag agccatcttc tttcaatgcc gttaccacgc tgtcaatcac
    17401 tcggtcggct tctactcgct gttcaactaa ctggcgggct tccatatctt ctttggcatt
    17461 tgccattgag gatttaatca tctgcgtaac ttcttcatcg gttaagccat aagacggttt
    17521 gatttgaatt gaagcctgca ctttggtcga tttttccatt gcagtcacgt ttaataaacc
    17581 gtctgcatca acttggtaag ttacccgaac ctgtgcaacc cctgctgcca ttgccggaat
    17641 gccacgcaaa gtgattttac cgagagagcg acaatcttcg actaattcac gctcaccttg
    17701 taaaacgtgg attgtcatcg ccgtttgacc gtctttaccg gtagtaaact cttgagctcg
    17761 agctactggg atagtagtgt tgcgtggaat aattttctcc accaaaccac ccatcgtttc
    17821 aataccaagc gaaagtggca caacatcaag caacaacata tcggaatccg gtttgtttcc
    17881 aactaaaata tccgcctgca ctgccgcacc aatggcaaca actttatccg ggtcgataga
    17941 ggttaatggc gttttaccga aaaactcgcc gacttgttca cgcacaaacg gtacacgggt
    18001 cgaaccgcct accattacca cttcacgaac ctcttctgct tcaactccgg catctttcaa
    18061 ggctcggcga caagtcatca gcgagcgttt tacaagcggt tgaattagct cattaaattg
    18121 cgaacggctt actacgcctg tccaatttgc aaatttcact tcaaattcaa ccgcttgcga
    18181 aagtgcgact ttggtttgag ttgccaaagt taataattca cgctgctcgc ttgccgtttg
    18241 aggtttgtaa tttgcctgtt cagcaatcca ttccgctaat aggtggtcga aatcatcgcc
    18301 accgagtgcg gtatcacctg cggttgctaa tacttcaaac acacctttgc gtaaacgcaa
    18361 aatagataaa tcgaaagtac cgccacctaa atcgtaaacg gcaatcacac cttcttgtcc
    18421 gctttctaaa ccataagcaa tcgctgctgc cgttggttca ttgagcaaac gtaatacatt
    18481 taaacccgca agacgtgcgg catctttggt actttggcgt tgtgcgtcat caaaataagc
    18541 cggtacggta attaccacgc ctgaaagctc accggctaaa cgcttttcgg caaattgtgc
    18601 caagtggctt aaaatatccg ccgaaacttc aatcgggctt ttattacctt gcgctgtttg
    18661 gattaaaggt aaaccgtttt cgctcgcgac aaattgataa ggtaaatttt gataacgttg
    18721 ctgaacatct gccagagaac gcccaatgag acgtttagct gaaatgacgg tatttttcgg
    18781 gtcaagtgca gctttggcaa aggcttcaat cccaactgtt ttttctgttt ccgcataatg
    18841 cactactgac ggcactaatg ctctttcttg ttcatcaagc aatacctgag tctgcccgct
    18901 acgcacagtt gccactaatg aattggttgt acctagatca attcctaccg ctaatttatg
    18961 ttgatgtggt gcagcggttt gaccgggttc agaaatttgt aataatgcca tt
//
LOCUS       DANLRK010000027        18960 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.027, whole genome shotgun sequence.
ACCESSION   DANLRK010000027 DANLRK010000000
VERSION     DANLRK010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 18960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18960
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.027"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            26..709
                     /locus_tag="PXA33_000709"
     CDS             26..709
                     /locus_tag="PXA33_000709"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011201466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HDL5120068.1"
                     /translation="MQLVFIRHGFSEWNAKNLFTGWRDVNLTERGIEEAKSAGQKLKA
                     AGFEFDIAFTSVLTRAIKTCNIVLEESDQLWIPQVKNWRLNERHYGALQGLDKKETAE
                     KYGDEQVHIWRRSYDISPPDLDPQDPNSAHNDRRYANLPKDLIPNAENLKITLERVLP
                     FWEDHIAPALISGKRVLVTAHGNSLRALAKHIEGISDADIMDLEIPTGQPLVYELDDN
                     LKVISKRYL"
     gene            complement(846..1403)
                     /gene="frr"
                     /locus_tag="PXA33_000710"
     CDS             complement(846..1403)
                     /gene="frr"
                     /locus_tag="PXA33_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HDL5120069.1"
                     /translation="MINEIKKDTQDRMEKSLEALKGHIAKIRTGRAQPSLLDGIQVEY
                     YGSATPLRQVANVVAEDARTLAVNVFDKSLISAVEKAILTSDLGLNPSSAGTTIRVPL
                     PPLTEERRRDLIKIVKAEGEQGKVAIRNVRRDANDQIKALLKDKEISENEQHKAEEDI
                     QKITDTFIKKVDEVLAAKEKELLDF"
     gene            complement(1455..2171)
                     /gene="pyrH"
                     /locus_tag="PXA33_000711"
     CDS             complement(1455..2171)
                     /gene="pyrH"
                     /locus_tag="PXA33_000711"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HDL5120070.1"
                     /translation="MSKPIYKRILLKLSGEALQGSEGFGIDPSILDRMALEIKELLAE
                     GVEVGVVLGGGNLFRGAKLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAEVNAKLM
                     SAFQLNGICDTYNWSEAIKMLREKRVVIFSAGTGSPFFTTDSAACLRGIEIEADVVLK
                     ATKVDGVYDKDPAKYADAKLYNKLTYAQVIEQELQVMDLAAFTLARDHGMPIRVFNMG
                     KPGALRNVVFGTEEGTTISE"
     gene            2263..3825
                     /locus_tag="PXA33_000712"
     CDS             2263..3825
                     /locus_tag="PXA33_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HDL5120071.1"
                     /translation="MKSKQLFGILVAISLATAASCLMLIGSGFFPNPSIFEVILGVFL
                     IIALASSKWTYYLLLLPISIAYAIYTPVGIKFGKPTYEYIASIFATDMMESKEFIAQL
                     PILNIAAGIAIVLAVIFYRKITNRYQIRFLRNKTFVISAFVLMLFSLAPFKFFQEFYN
                     SSLKVKKELEVLNNLTLDSQWGQSTLSSTSNYDDYVLIMGESARKDYHHAYGYPAANT
                     PFMSSAKGTLIDGLTSGGTNTIASLRLMLTKPDTEKWEANYGLTLIDLIKSTGIKTYW
                     ISNQGYLGNYDTPISSIGNKSDVKIFTKAGDSLNTNVSDFELLPHFAKVIEEPAQGKR
                     FIFLHLYGSHPITCDRLTDYPKMFDDDKLPKRYFNVNCYVSSIKKTDELLKRVYDLLV
                     KNQENHQRSFSMVYLSDHGLSHEVSKENIVIHNGKDRSKLHYDIPLFKISSDDTQRNE
                     YKAFKSGLNFTNGIANWIGIENPLLDKEINLFNEQNDKSDYGLKKIIETQSTVEDPAV
                     PIPESYLYKATN"
     gene            complement(3889..4740)
                     /gene="tsf"
                     /locus_tag="PXA33_000713"
     CDS             complement(3889..4740)
                     /gene="tsf"
                     /locus_tag="PXA33_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HDL5120072.1"
                     /translation="MAEITASLVKELRERTGAGMMECKKALVEANGDIELAIDNMRKS
                     GQAKAAKKAGRVAAEGVILARVASGFGVLVEMNCETDFVAKDAGFLELANEVADFAVA
                     NKGTTIEALQAQFEEKRAALVAKIGENMNIRRVQYLEGEVIAQYLHGAKIGVLVAGAG
                     SEDELKKVAMHVAASKPEFVNPEDVSADVVAKEREIQIEIAMNSGKPKEIAEKMVEGR
                     MAKFTGEVSLTGQPFVMDPSQSVGQYLKSVNTSVSNFIRLEVGEGIEKVEEDFAAEVA
                     KITGGNA"
     gene            complement(4836..5555)
                     /gene="rpsB"
                     /locus_tag="PXA33_000714"
     CDS             complement(4836..5555)
                     /gene="rpsB"
                     /locus_tag="PXA33_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005645552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HDL5120073.1"
                     /translation="MAQVSMRDMLNAGVHYGHQTRYWNPKMKPFIFGARNGVHVINLE
                     KTLPLFNEALAELTRIASNNGKVLFVGTKRAASDAVKAAAVESQQFYVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQTQDGTFDKITKKEALMRTRELEKLELSLGGIKDMNGLPDA
                     IFVIGADHEHIAIKEANNLGIPVFAIVDTNSTPDGVNYIIPGNDDATRAIQLYLDSAV
                     AAIKEGRGVETVEVAEEASAE"
     gene            5900..6928
                     /locus_tag="PXA33_000715"
     CDS             5900..6928
                     /locus_tag="PXA33_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL5120074.1"
                     /translation="MKKTLSALAIAAATFTSTSTLAANEVNVYSYRQPYLIEPMLKNF
                     EKDTGIKVNIIFADKGLVDRVKQEGELSPADVLLTVDISRVMEIVNADLAQKIDSKVL
                     EKNIPAQFRDSNDQWFGLTTRARVIYTSKDRVGKLPAGFDYLDLAKPEYKGKVCVRSG
                     KNSYNVSLFAAMIEHYGIEKTKAFLEGLKANLARKPQGGDRDQVKAIKEGICDYSIGN
                     SYYYGKMLDDEKQKSWAEAAIINFPSGEHGTHKNISGVVIAKHSPNKANAVKLIEYLS
                     GEKAQGLYAELNHEYPVKEGIEPSAIVKGWGTFKSDTIKLEDIAKNYEAALKLVDEVK
                     FDDFSEKK"
     gene            7051..8697
                     /locus_tag="PXA33_000716"
     CDS             7051..8697
                     /locus_tag="PXA33_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDL5120075.1"
                     /translation="MRSQFCWKSIAALTTFIVLLPLIAICYHALQGDNENLAHLWQTM
                     LPIYLKNSTLLVLGTVFLALIFALPTAWIVANYRFWGQRTLQWLLCLPLAMPAYLIAY
                     LYTDLLDYSGAFQGWLRSIFGWTHPQDYWFPQIRTLYGACFVLALVLYPYIFLLVRVA
                     LLEQSENLTHSAKMLGASPSQLFRRITLPLIRPSVAVGIALVAMETLGDFGTVAYFAV
                     PTLTTAIYDSWLGYHDLGTASQIAIFMLLMIFLFLSLEQYSRRKQKSYQRGYEKKSAF
                     KFLTGWKLALALAWCWGLLALAFFIPFGRLLYWAYDYFEQAWNLDFVQFSLNSLKVSI
                     IASVITVLIALLLHFSKRLNTRKILVKNDRLSLQLASLGYAVPGTVLAIGLLSPLTFA
                     DHQLHAWLKSLELSPVGLIFSGSLFALVLAYTIRFLAMAIGSLETSLNKISPSLDMAS
                     QTLGKNGSAMLVKIHIPLIYKGILTAGLMVFIESMKELNASLLLRPFNFDTLATYVFT
                     FTSDEQLERAALPAIVLVLVGLLPVIWLTRSLISQSEKER"
     gene            8707..9717
                     /locus_tag="PXA33_000717"
     CDS             8707..9717
                     /locus_tag="PXA33_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL5120076.1"
                     /translation="MSVLTINHLNLNFGSTKVLQNLHLAVNQNEIICLLGASGCGKTT
                     LLKAIAGLLPIEQGDIHLAGECLKSKAVEERQIGLIFQDYALFPHLTVADNIQFGLSK
                     LPKVQQKAITEKMLSVVHLQGFESRFPHELSGGQQQRVAIARALACQPELLLLDEPFS
                     NIDSQTRYAMIQEIKQILKSQNVPAIFVTHSKEEAFAFADKIAVMDQGKIIQFGKPNE
                     LYHSPVNPFVADFLGGTNYLNCNVTEDNVLCSPIGEFRLFPEMNVQKGRYRWLLRPEQ
                     IILKPNELGLGKVVGKLFLGLYYRYQVEIKGIEIVTYQSQELKIGEQVEVGFQVREFI
                     LF"
     gene            9727..10353
                     /locus_tag="PXA33_000718"
     CDS             9727..10353
                     /locus_tag="PXA33_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HDL5120077.1"
                     /translation="MKIEVWSDYACPFCYIGKRHLEQALAQFEGEVEVVFRAFELDPH
                     ANGEPEGDIQQRLMRKYQKTAEQADEMIRYVEQAGKQAGLDLRYRTTQYTRTFEAHRL
                     AKFAESKDLGEAMVERLFKAYFTDNTILAKRTELISLALDIGLERDEIAQLLTGDDFG
                     HEVREDERVAHKYGIHSVPFFVINEKLGVSGAQPPEILLDAIKQALQK"
     gene            complement(10415..11905)
                     /locus_tag="PXA33_000719"
     CDS             complement(10415..11905)
                     /locus_tag="PXA33_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HDL5120078.1"
                     /translation="MSLYQIARTETGKVISLNAKMANRHGLIAGATGTGKTVTLRKLA
                     ESLSDDGVPVFLVDVKGDLSGSVQAGNFSGKIAERILQFNLGGEDYLKGYPVSFWDVF
                     GTSGIPLRITISEMGPMLLSRLLNLNDTQGGLLNLVFRVADDRGLLLIDLKDLRALLK
                     FVADNAKEFQLEYGHISAASVGAIQRALLRLENEGADNLFGEPALDLHDWIQTRNGRG
                     VINMLNAEKLINSPRVYSAFLLWFMAELFENLPEVGDPEKPTFVLFFDEAHLLFDDAP
                     KALVDKIEQVVRLIRSKGVGVYFVTQNPLDIPDSVLGQLGNRIQHALRAFTPRDQKAV
                     KSAAETFRANKEVNVVEAITQLGVGQALVSVLDEKGMPTPVEIAYIYPPKSQLAPLAT
                     GERDSLVKQDDLYHHYSQYVDNESAFELLNAKAEAIKAEQAAAEEANNDFFGGILSSI
                     FGSKKKKDQSITEQVVSQVAHSVGRNLRNQVTKQIMRGILGAITKK"
     gene            complement(11964..13199)
                     /gene="rhlB"
                     /locus_tag="PXA33_000720"
     CDS             complement(11964..13199)
                     /gene="rhlB"
                     /locus_tag="PXA33_000720"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HDL5120079.1"
                     /translation="MSNHLTEHRFIDFPIHANVLNALDSKGFEFCTPIQAKTFPITLK
                     GKDIAGQAQTGTGKTLAFLIATFNHLLNNEIETSKNQPRALILAPTRELAVQIGADAE
                     LFLKHSDLKLALAYGGDGYDKQVQEIEKGVDILVGTTGRVIDYVKQGIINLDKIQVIV
                     LDEADRMFDLGFIKDIRYLMRKAPKPSERLTLLFSATLSAKVRELAYEDMDNAEYIEI
                     EPLQKTGHRIKEELFYPSNEDKMALLLTLLEEEWPERCMIFANTKHKCEEIWQYLSAD
                     GHRVGMLTGDIPQKKRLSLLDNFTKGNLDILVVTDVAARGLHIPDVTHVFNYDLPDDK
                     EDYVHRIGRTGRAGESGHSISFACERYAVNLPAIEEYIGHQIPVSQYDKESLLTLPKV
                     NRSRSKKTFLAKTKKNSRN"
     gene            complement(13287..14009)
                     /gene="recO"
                     /locus_tag="PXA33_000721"
     CDS             complement(13287..14009)
                     /gene="recO"
                     /locus_tag="PXA33_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HDL5120080.1"
                     /translation="MTIDQWQRAFVLHRREYSENSLLVEFFTEHHGRITLLAKGARRA
                     RSPLKAVLQPFTPLLLRWSGKGDLKTLTKAEPAALTLPMETLSLYSGFYINEVLARVL
                     ENQTAYPELFQHYLRCITELATQKQIEPILRTFEFHTLKALGYGVDFTHCAATGLPVD
                     PKMTYQFQENQGFIASLLQNNQSFLGSDLLAFAQLDFSEKSTQQAAKRFTRMALKPYL
                     GSQPLKSRELFQSILPNKLKSG"
     gene            complement(14239..15156)
                     /gene="era"
                     /locus_tag="PXA33_000722"
     CDS             complement(14239..15156)
                     /gene="era"
                     /locus_tag="PXA33_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013525392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HDL5120081.1"
                     /translation="MTEQIQTPAKTYCGFIAIVGRPNVGKSTLLNKILGQKISITSRK
                     AQTTRHRIVGIHTEDQYQAIYVDTPGLHIEEKRAINRLMNRAASSAIGDVDLIIFVVE
                     GTKWTEDDEMVLNKLRSAKAPVLLAINKIDNIKEKEELLPHITELSQKFDFAEILPIS
                     AQRGKNVHILQKFVRQSLRQGEHHFPEEYVTDRSQRFMASEIIREKLMRFTGEELPYS
                     VTVEIEQFKMNERGVYEINGLILVERDGQKKMVIGAGGQKIKTIGIEARKDMERLFDN
                     QVHLELWVKVKAGWADDERALRSLGYMDE"
     gene            complement(15259..15933)
                     /gene="rnc"
                     /locus_tag="PXA33_000723"
     CDS             complement(15259..15933)
                     /gene="rnc"
                     /locus_tag="PXA33_000723"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HDL5120082.1"
                     /translation="MQLERLQKKLGYQFHNLDYLIQALTHRSAATKNNERLEFLGDSI
                     LNFTIGKVLYDKFPKANEGELSRMRATLVREQTLAIIARKFDLGDYLKLGPGELKSGG
                     FRRESILSDCVEAIIAAIYLDQDMAKAMEKVEDWYSDLLKDISPGDSQKDPKTRLQEF
                     LQGRKLPLPEYDVLDIKGEAHNQTFRVTCKVINVEEVFIGIGTSRRKAEQNAAEKALA
                     VVKTKK"
     gene            complement(15979..16938)
                     /gene="lepB"
                     /locus_tag="PXA33_000724"
     CDS             complement(15979..16938)
                     /gene="lepB"
                     /locus_tag="PXA33_000724"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDL5120083.1"
                     /translation="MAQFMPIIFLGIIYGIWQWLDSMQLPNTISIILVALVIICGGFW
                     AFYKFSADPRRKQAIVLQQKRLGRELTAEEIAEIQPKSAVGEFFASLFGILFFVTVLR
                     SFLFEPFQIPSGSMEPTLRVGDFLVVNKFSYGIKDPIWQNTLIETGKPERGDVIVFKA
                     PAQPHVDYIKRVVAVGGDKIKYDFATQQLTVTKANGETKVFEYSEGKPNPEFFYHGEM
                     QVERTEKGNVTHQILNHPMAFNYAPYFYQQEGQAEGEWVVPQGYYFVMGDNRDNSEDS
                     RFWGFVPERNIVGKATFIWLSLDKKANEFPTGLRFSRMFTSIN"
     gene            complement(17025..18818)
                     /gene="lepA"
                     /locus_tag="PXA33_000725"
     CDS             complement(17025..18818)
                     /gene="lepA"
                     /locus_tag="PXA33_000725"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HDL5120084.1"
                     /translation="MQNIRNFSIIAHIDHGKSTLSDRLIQTCGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLNYKAKDGEIYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAIEIDLEVVPILNKIDLPAAEPERVAEEIEDIVGIDAIDAVR
                     CSAKTGLGIEDVLEEIVKKMPAPEGDAEAPLQALIIDSWFDNYLGVVSLVRVKNGVLK
                     KGDKIKVMSTGQSYNVDRLGIFTPKQVDTTELKTGEVGWVVCAIKDILGAPVGDTLTH
                     HHNPATEVLPGFKKVKPQVYAGLFPISSDDYEAFRDALGKLSLNDASLFYEPETSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVVQTSGETIYVDSPSKLPA
                     INNIAEIREPIAECNMLVPQEFLGNVITLCVEKRGVQTNMVYHGNQIALTYEIPMGEV
                     VLDFFDRLKSTSRGYASLDYGFKRFQTADMVRVDIMINGERVDALAIIVHRANAAYRG
                     RELVEKMRELIPRQQFDIAIQAAIGNHVIARSTVKQLRKNVLAKCYGGDVSRKKKLLQ
                     KQKEGKKRMKSLGNVEVPQEAFLAILHVGKD"
BASE COUNT         5519 a         4053 c         3498 g         5890 t
ORIGIN      
        1 ttttactcaa agctaaggag tcattatgca attagtattt atccgtcacg gtttcagtga
       61 atggaatgct aaaaacttat ttaccggttg gcgtgatgtt aatttaacag aacgtgggat
      121 tgaagaagca aaatctgccg gtcaaaaatt aaaagcagcc ggttttgaat ttgatattgc
      181 gtttacttcg gttttaaccc gtgcgatcaa aacttgtaac atcgtattag aagaatcaga
      241 ccaattatgg atccctcaag tgaaaaactg gcgtttaaac gagcgtcact acggtgcatt
      301 acaaggttta gacaaaaaag aaactgctga aaaatatggt gatgaacagg ttcacatctg
      361 gcgtcgttct tatgacatct ctccaccgga tttagaccca caggatccaa actcagcaca
      421 taacgaccgc cgttatgcta acttaccaaa agatttaatt ccaaatgcag aaaacctcaa
      481 aatcaccctt gagcgtgtgt taccattctg ggaagatcat attgcacctg cattaatttc
      541 aggtaaacgc gtattagtta ctgctcacgg taactcatta cgtgcattag caaaacacat
      601 cgaaggcatt tctgatgcag acattatgga cttagaaatc ccaaccggtc agccattagt
      661 gtatgaacta gatgacaact taaaagtaat cagcaaacgt tacttataat tggtaaattt
      721 ccgataaaaa ataaccgctt gtagtgcaac acattacaag cggttatttt ttgttaattt
      781 tttgcaaatg gcaaagaaca aaaataaagg cgaatcatac attcgcctta ttgttttaaa
      841 gtagattaga aatctaacaa ctctttctct tttgctgcta atacttcatc aactttttta
      901 atgaaggtat cggtgatttt ttgaatatct tcttcagctt tgtgttgttc gttttcgctg
      961 atttctttat cttttaataa cgctttgatt tggtcattag catcacgacg aacattacga
     1021 attgccactt tgccttgctc accttctgct ttaacgattt tgattaaatc acgacgacgt
     1081 tcttccgtta atggtggaag cggaacacga atggttgtgc cggcagaaga tgggtttaaa
     1141 cctaaatctg aggttaaaat tgctttttct accgcagaga ttaaagattt atcaaatacg
     1201 tttaccgcta aagtacgggc atcttcagct accacgttcg ctacttgacg taatggagtc
     1261 gcagagccgt aatattccac ttgaataccg tctaataaag aaggttgggc acgacctgta
     1321 cgaattttcg caatatgccc ttttaaagcc tcaaggcttt tttccatacg atcttgggta
     1381 tctttcttga tttcgttaat cattgatttt ttatcctttt atttagtaac tgagagttaa
     1441 acagagaaaa ttaattattc agaaatggtt gtaccttctt cagtaccgaa taccacattg
     1501 cgtaacgccc ccggtttgcc catattaaag acacgaattg gcattccgtg gtcacgagca
     1561 agcgtgaatg ctgctaagtc cattacttgt aattcttgct caataacttg cgcatacgtt
     1621 aatttattat atagttttgc atctgcgtat tttgctgggt ctttatcata aacgccatca
     1681 actttagttg cttttaatac cacatcagct tcaatttcaa taccacgcaa gcaagcagct
     1741 gaatcggtgg taaagaacgg gctacctgta ccggcagaga aaatcaccac acgtttttca
     1801 cgcaacattt taattgcttc agaccagttg taagtgtcgc aaatcccatt taattggaac
     1861 gcagacatta atttagcatt tacctctgca cggtgtaagg catcacgcat agccaaaccg
     1921 ttcattacgg tggcaagcat acccatatgg tcgcccacta cacggttcat tcctgctttg
     1981 gctaattttg caccacggaa taagttaccg ccgccaagta ccacaccaac ctcaacgcct
     2041 tcagcaagta attctttaat ctctaacgcc atacgatcca aaatagacgg gtcgatacca
     2101 aaaccttcac tgccttgtag ggcttcacca cttaatttga gaagaattcg cttataaata
     2161 ggtttgctca ttttaattgc ctcttaactg tatggactaa acacaaaatt acgttattat
     2221 agaacaatcc aatataacga taaactaaaa atattcacta caatgaaatc caaacaactt
     2281 ttcggcattc ttgtcgctat ttcgctggca actgctgcta gctgtttaat gctaatcgga
     2341 tcaggctttt ttccaaatcc gagtatcttt gaagtcattt tgggcgtatt cctcatcatc
     2401 gctcttgcca gctctaaatg gacttattat ttactcttac tgccaatcag catcgcttat
     2461 gcaatttata ccccggttgg gataaaattc ggtaaaccga cttacgagta tatcgcctct
     2521 atttttgcta ccgatatgat ggaaagcaaa gagtttattg ctcaactgcc gattttaaat
     2581 attgcggcgg gcattgcgat tgtactagcg gtcatttttt atcgaaaaat taccaatcgc
     2641 tatcaaatcc gttttttacg caataaaaca ttcgtgattt cggcttttgt attaatgctg
     2701 ttttcccttg caccgtttaa attttttcag gagttctaca attcgagcct aaaggtaaag
     2761 aaagaattgg aagtgctgaa caatttaacg ctagattcac aatggggaca atcaaccctc
     2821 agctcaactt caaactacga tgattatgta ctgattatgg gcgaaagtgc gagaaaggat
     2881 tatcaccacg cctatggcta tccggctgcc aatacgcctt ttatgagttc ggcaaaagga
     2941 accttgattg acggtttaac ctccggtggc acaaacacta tcgcctcatt acgcttaatg
     3001 ctcactaagc ccgatacgga aaaatgggaa gcaaattatg ggttaacctt aattgattta
     3061 atcaaatcta ccggtatcaa gacctattgg atctcaaatc aaggctattt gggcaattac
     3121 gatacgccga tttcctctat cggtaataaa agtgatgtca aaattttcac taaagccggc
     3181 gattcattaa ataccaatgt gagcgatttt gaattgttac cgcattttgc taaagtgatt
     3241 gaagaacctg ctcaagggaa gcgtttcatc tttctgcatt tatatggctc gcatcctatt
     3301 acctgcgatc gtctcaccga ttacccgaaa atgtttgacg atgacaaact gccaaaacgc
     3361 tactttaatg taaattgcta cgtgtcatct atcaagaaaa ccgatgaatt gctgaaacgg
     3421 gtatatgatt tgctagtgaa aaaccaagaa aatcaccagc gttcattctc aatggtttat
     3481 ttgtctgatc acggtttatc gcacgaggtt tcaaaagaga acattgttat ccacaatgga
     3541 aaggatagaa gtaaattaca ctacgatatt ccgctgttta aaatttcaag cgatgatacc
     3601 caacgtaatg agtataaagc ctttaaatcc ggcttaaact tcactaatgg tattgcgaat
     3661 tggattggta tcgaaaatcc gttattagat aaagaaatta atctttttaa cgaacaaaac
     3721 gataaaagtg attatgggct aaagaaaatc attgaaacgc agtctactgt agaagatcca
     3781 gctgttccta ttcctgaaag ctacttatat aaagcgacta attagcaaaa gtaagtaaca
     3841 aaaaagcagg ctttaagcct gcttttttag tttttagtat cagaaaaatt aagcgttacc
     3901 gccagtgatt ttcgctactt ctgctgcgaa atcttcttct actttctcaa taccttcacc
     3961 tacttctaaa cgaataaagt tagaaactga agtatttact gattttaaat attgacctac
     4021 agattgagat ggatccatta caaacggctg accggttaat gaaacttcac cggtgaattt
     4081 agccatacgg ccttcaacca ttttttctgc gatttctttt ggtttaccag aattcatcgc
     4141 aatttcaatt tgaatttcac gttcttttgc tactacatca gcagaaacat cttctgggtt
     4201 tacaaattca ggtttgcttg cagcaacgtg cattgctact ttcttaagct catcttcaga
     4261 gcctgcacct gctactaata caccaatttt cgcaccgtgt aagtattgag caataacttc
     4321 gccttctaag tattgaacac ggcgaatgtt catattctca ccgattttcg caactaatgc
     4381 cgcacgtttt tcttcaaatt gtgcttgtaa tgcttcgata gttgtacctt tatttgcaac
     4441 tgcaaaatca gcaacttcgt tcgctaattc taagaaaccg gcatcttttg caacgaagtc
     4501 agtttcacag ttcatttcaa ctaatacgcc aaaacctgaa gcgacacgag caaggattac
     4561 accttcagct gcaacacgac ctgctttttt agccgctttc gcttgaccgg atttacgcat
     4621 attatcgatt gctaattcaa tatcaccgtt cgcttcaact aatgcttttt tacattccat
     4681 catacctgcg ccggtacgtt cacgaagttc tttaactaat gatgctgtga tttcagccat
     4741 tttaaattcc ccttatttga tatggtaaaa caggctctca cctgcctaga aatttcattg
     4801 tttagggcag gttaaaacct gccccctcaa ctgaattatt ccgcagaagc ttcttctgct
     4861 acttcaacag tttcaacacc gcgaccttct ttgattgcag caactgctga atctaagtaa
     4921 agttggatag cacgtgttgc atcatcgtta ccgggaatga tgtagttaac gccatctgga
     4981 gttgagttag tatcaacaat cgcaaatacc ggaataccta agttgtttgc ttctttaatt
     5041 gcgatatgtt cgtgatcagc accgataaca aaaattgcat ccggtaaacc gttcatatct
     5101 ttgataccgc ctaagcttaa ctctaatttt tctaactcac gagtacgcat taacgcttct
     5161 tttttagtga ttttgtcaaa agtaccgtct tgagtttgag tttctaaatc ttttaagcgt
     5221 ttgattgatt gacgaactgt tttccagtta gtcaacatac cacctaacca acggtggttt
     5281 acgtagaatt gttggctctc tactgccgct gctttaacag catcacttgc tgcacgtttt
     5341 gtaccaacaa ataatacttt accattgtta ctagcaatgc gagttaattc cgctaatgct
     5401 tcgttgaata aaggtaaagt tttttctaag ttgattacgt gaacaccatt acgagcacca
     5461 aagatgaatg gtttcatttt tgggttccag taacgtgttt ggtgaccata gtgaacgcct
     5521 gcgttaagca tatcgcgcat agaaacttgt gccataagaa ttttccttca aagttgtttg
     5581 ttttgccact tatgaacggc aaaacggggt ttagcctcca catatcctag aaaatcaatc
     5641 tgttaaagac accccaattc ctagcttgtg atatgtgtgt gttttagtgt taaaaaataa
     5701 atgtgtacgt tttgcctgaa tagacaaaaa cggggcgtat tatgccaaaa aacaaacaat
     5761 ttgccaatta ttttattgct ctcttcgcat tactaaatca agctcaaaaa taaaaaccgt
     5821 tctcattatt gactaaaaaa atggggaggt ttagaatgtc tttcattctg ttatgaaaac
     5881 ctgtctgaag gagactatca tgaaaaaaac actttctgca cttgctattg ctgcagccac
     5941 ttttacatca acttcaacgc ttgctgcaaa tgaagtgaat gtttattctt accgccagcc
     6001 ttacttaatt gagccaatgc taaaaaactt tgaaaaagat actggcatta aagtaaacat
     6061 catcttcgct gataaagggt tggttgatcg tgtaaaacaa gaaggtgaat taagtcctgc
     6121 tgatgtgctt ttaacagtag atatttcacg cgtaatggaa attgttaacg ctgatcttgc
     6181 acaaaaaatt gactctaaag tgttagaaaa aaatattcct gcacaattcc gtgactctaa
     6241 cgaccaatgg tttggtttaa ccacacgtgc tcgtgttatc tacacgtcaa aagatcgtgt
     6301 aggtaaatta ccagcaggct ttgactacct agatttagct aaaccagagt ataaaggtaa
     6361 agtctgcgtg cgttcaggca aaaactctta caatgtctca ctattcgcgg cgatgatcga
     6421 acattacggc attgaaaaaa ccaaagcatt cttagaaggc ttaaaagcga acttagcacg
     6481 aaaaccacaa ggtggtgacc gtgatcaagt aaaagcaatt aaagaaggca tctgtgatta
     6541 ctctatcggt aacagctact actacggcaa aatgttagat gatgagaaac aaaaatcgtg
     6601 ggctgaagca gcgattatta atttcccgag tggtgaacac ggcacgcaca aaaacatcag
     6661 cggcgtagtt attgcaaaac actcaccaaa taaagcaaac gcggtgaaat taattgaata
     6721 cctaagtggc gaaaaagcac aaggtttata tgctgaactt aaccacgaat atccagtaaa
     6781 agaaggcatc gagccatcag caatcgtaaa aggttggggt acgttcaaat cagataccat
     6841 caaacttgaa gatatcgcaa aaaattacga agccgcatta aaattagttg atgaagtgaa
     6901 atttgacgat ttctctgaga aaaaataatc aaccgataag cggtcatttt tccacaaaaa
     6961 tttgcaaaat tttgttaaaa tacaaccgct tgcaaaagga cgcattttgc gtccttttat
     7021 ttttttatga aataacaggc ttaaagaaaa atgcgatcac aattttgctg gaaaagtatt
     7081 gctgccctaa caacttttat cgttttgctt cctcttattg caatttgcta tcacgcattg
     7141 caaggggata atgaaaacct tgctcacctt tggcaaacaa tgttgccaat ctatcttaaa
     7201 aactcaactt tattagtatt aggaaccgtt ttccttgcac tgatttttgc tctgcctacc
     7261 gcttggattg tggcaaatta ccgtttttgg gggcaaagaa ccttacaatg gttactttgt
     7321 ttgcctctcg ctatgcctgc ttatttaatt gcctatttat ataccgattt attagattac
     7381 tcaggtgctt ttcaaggctg gctgcgtagt atttttggct ggactcatcc gcaagattac
     7441 tggtttcctc aaattcgtac attgtatggt gcttgctttg tgcttgcgtt agtactatat
     7501 ccatacattt ttttattagt gcgagttgcc ttattagaac aatctgaaaa cttaacacac
     7561 agtgcaaaaa tgctcggggc ttcgccctct cagttgttcc gtcgaattac gctgccatta
     7621 attcgacctt ctgttgccgt aggcattgca ttagtggcaa tggaaacgct tggcgatttc
     7681 ggtactgtgg cctattttgc cgtgccaaca cttactacgg caatttatga ttcgtggcta
     7741 ggctatcacg atcttggtac tgccagccag attgcgatct ttatgttgct aatgattttc
     7801 ctgtttttaa gtttagagca atatagtcgc cgtaaacaaa aaagctatca acgtggctat
     7861 gaaaagaaat cagcctttaa atttttaact ggttggaaat tagcattagc ccttgcttgg
     7921 tgctggggtt tattggcgtt agcctttttc attccgtttg gcaggttgct ctattgggca
     7981 tacgactatt tcgagcaagc gtggaattta gactttgtcc aattttcgct taatagtttg
     8041 aaagtatcga tcatcgcctc cgtgataacg gtactgattg ctctacttct gcatttttct
     8101 aaacgtttga atacccgcaa aattttagtc aaaaatgacc gcttgtcatt acaacttgcc
     8161 tcactgggct atgcggttcc tggcacagta ttagcgatcg gtttactttc gcctttaaca
     8221 tttgccgatc atcaactcca tgcttggcta aaaagccttg aattatcacc cgttggattg
     8281 attttttcag gctcactctt tgcattagta ttagcttata ccatccgatt tttagcaatg
     8341 gcaattggca gccttgaaac ttcgctaaat aaaatctctc catcgctgga tatggcgagc
     8401 caaacgcttg gtaaaaatgg ctcagcaatg ttagtaaaaa ttcatatccc tttaatttat
     8461 aaaggtattt taactgcagg attaatggtc tttattgaga gtatgaaaga gctaaatgct
     8521 tctctgctac ttcgcccatt taattttgat acactcgcaa cctatgtgtt tacctttaca
     8581 tcagacgagc agcttgagcg tgctgctctg cctgccatcg tattggtgtt agtcggttta
     8641 ctgcctgtta tttggcttac cagatccctt atttcgcaat ctgaaaaaga aagataaagg
     8701 aattttatgt ctgttttaac tattaatcat ctcaacctca atttcggcag tacaaaggtt
     8761 ttacaaaatc tccatcttgc tgtgaatcaa aatgaaatta tctgtttgct aggtgcaagt
     8821 ggctgtggga aaactacact gctcaaagcc attgcaggtt tattgccaat tgagcaggga
     8881 gatattcatt tagcgggtga atgcctaaaa agtaaagccg tggaagagcg acaaatcggg
     8941 ctgattttcc aagattacgc tctttttcca cacttgacgg tagccgataa tattcaattt
     9001 ggcttatcca aattgccaaa agtacaacaa aaagcaatta ccgaaaaaat gctttcagtt
     9061 gtgcatttac aaggctttga aagccgtttt ccacacgaac tttcgggcgg gcaacaacag
     9121 cgtgtcgcta ttgcccgagc attagcctgc cagccagaat tgctactgtt agatgagccg
     9181 ttttccaata ttgacagcca aactcgctat gcaatgattc aagagatcaa gcaaatctta
     9241 aaaagccaaa atgtgcccgc tatttttgta acccatagca aagaagaagc ctttgcattt
     9301 gccgataaaa ttgccgtgat ggatcaaggg aaaattattc aatttggtaa gccaaacgag
     9361 ctataccatt ctccagttaa tccatttgtg gcagattttt taggtggcac aaactatctt
     9421 aactgtaatg taacggaaga caacgtgtta tgcagcccta ttggcgagtt ccgactcttc
     9481 cctgaaatga acgttcagaa agggcgttac cgttggcttc ttcgcccaga acagattatc
     9541 ttaaaaccca atgagctagg tttaggtaaa gtggtaggca aactgttttt aggcctatac
     9601 taccgctatc aggttgaaat taaaggcatt gagatagtaa cttaccaatc tcaagagctg
     9661 aaaattggcg aacaagtgga agtgggcttc caagtaagag agtttatcct attttaagga
     9721 gaatatatga aaattgaagt ttggtcagat tatgcctgcc cgttttgtta catcggcaaa
     9781 cgccacttag agcaagccct agcccaattt gaaggcgagg ttgaagtggt tttccgagcc
     9841 tttgagcttg atccgcacgc taacggcgaa ccggagggcg atattcagca acgcctaatg
     9901 cggaaatatc aaaaaaccgc agaacaagct gatgaaatga ttcgttatgt ggaacaagcc
     9961 ggcaaacaag ctgggctaga tttgcgttac cgcaccaccc aatacactcg cacctttgaa
    10021 gctcatcgct tggcgaaatt tgcagaaagt aaagacttag gcgaagcaat ggttgaacgt
    10081 ttattcaaag cctattttac cgataacact attctagcta aacgcacaga gcttatcagt
    10141 ttagccttag acattggctt agagcgtgat gaaatcgctc aactgctcac aggtgatgat
    10201 ttcgggcacg aagtccgtga agatgagcga gtcgctcaca aatatggcat tcacagcgta
    10261 ccgttttttg tgatcaatga aaaactcggg gtatccgggg ctcaaccgcc agaaatatta
    10321 cttgacgcta tcaaacaggc attgcaaaaa taatccgaaa aaagaccgct tgttaagcta
    10381 aacaagcagt cttattcttc ttataattta caatttattt tttggtgatc gcacctaaaa
    10441 taccacgcat aatttgtttg gtgacttggt tgcgtaaatt tctgccaact gaatgcgcca
    10501 cttggctaac cacttgttcg gtaatcgatt gatctttttt cttcttcgag ccaaaaatgg
    10561 aagagagaat accgccaaag aagtcattgt ttgcttcttc tgccgcagcc tgctctgctt
    10621 taattgcttc tgctttcgca ttaagtaatt caaacgcaga ttcattatcg acatattggc
    10681 tgtagtgatg atagagatca tcttgcttca ctaaactatc acgttcgcct gtggctaatg
    10741 gagctaattg gctttttggt ggatagatat aggcaatttc tacaggcgtt ggcatacctt
    10801 tttcatctag caccgaaacc aacgcttgcc ccacaccaag ttgcgtgatg gcttctacca
    10861 catttacctc tttattcgct cggaaagttt ccgctgctga ttttaccgct ttttgatcac
    10921 gtggcgtgaa agcacgcaaa gcgtgttgga tacgattgcc aagctgccct aatactgaat
    10981 caggtatgtc caacggattt tgcgtaacaa aataaacacc cacccctttt gagcgaatta
    11041 aacgaaccac ctgttcaatt ttatccacca atgccttagg ggcatcgtca aacaataaat
    11101 gagcttcatc aaagaataaa acaaacgtcg gtttttctgg gtcgccaact tctggcaaat
    11161 tctcaaagag ctctgccata aaccaaagca ggaatgcact gtacactctt ggtgagttaa
    11221 ttaatttttc tgcattcaac atattgatta caccacgccc attacgggtt tggatccaat
    11281 cgtgcaaatc taatgcaggc tcaccaaata aattatctgc cccttcattt tcaagcctta
    11341 ataacgcacg ctgaatagct ccaacgcttg ctgcagaaat atgcccatat tccagttgga
    11401 attctttcgc attatctgcg acaaatttaa gcaacgcacg caaatctttt aaatcaatta
    11461 gcaataaacc tctgtcatct gccacacgaa aaacgagatt taacaagcct ccttgggtat
    11521 cgtttaaatt gagtaaacga gaaagtaaca tcggtcccat ttcggaaata gtgattcgaa
    11581 gtgggatacc tgatgtacca aatacgtccc aaaacgaaac tggatagccc tttaagtaat
    11641 cttctccccc taaattaaac tgtaaaatgc gttctgcaat ttttcctgaa aaattacccg
    11701 cttgtaccga gcccgaaaga tcacctttta catctactaa aaatactggc acgccatcat
    11761 cactcaaaga ttctgccaat ttgcgtaaag taaccgtttt acctgtaccc gtagccccag
    11821 caattaaacc gtgccgattt gccattttgg cattaagcga aataactttg cctgtttctg
    11881 ttcgagcaat ttgatacaaa ctcatctttc attctcttaa aggtaaatta attttttgtt
    11941 ctcatcatat aaaaatttat cacttaatta cggctattct ttttcgtttt tgccaagaac
    12001 gtttttttac ttcttgagcg gttcactttc ggtaaagtca gtaaggattc cttgtcgtat
    12061 tgactcacag gaatctgatg cccaatatac tcttcaattg caggcaaatt aaccgcataa
    12121 cgctcgcaag caaaactaat tgaatggccg ctctcccctg ctcgccccgt tctaccaata
    12181 cgatgaacgt aatcttcttt atcatcaggc agatcataat tgaaaacgtg cgtgacatct
    12241 ggaatatgca atccacgagc cgcaacatct gttaccacta aaatatccaa attgcctttg
    12301 gtgaaattat ccagcaaaga aaggcgtttt ttctgcggaa tatcgccggt tagcattcca
    12361 acccgatgtc cgtctgccga taaatactgc cagatctctt cgcatttatg cttggtgttg
    12421 gcaaaaatca tacaacgctc cggccactct tcctcaagca atgtcagcaa caatgccatt
    12481 ttgtcttcat tcgaggggta aaatagctct tctttaatac gatgtccggt tttttgtagt
    12541 ggctcaattt caatatattc cgcattatcc atatcttcat aggcaagttc ccgcactttt
    12601 gccgaaagcg ttgccgaaaa gagcaaagtt aaacgttctg aaggcttagg tgcttttcgc
    12661 attaaataac ggatatcttt aataaaacca agatcaaaca ttcgatctgc ttcatctagc
    12721 acaatcactt gaattttgtc taaattgata atgccttgtt ttacatagtc aatcactcga
    12781 cctgttgtac cgacaagaat atctacccct ttttcaattt cttgaacttg tttgtcgtaa
    12841 ccatcaccac cgtatgccaa agctagtttt aaatcagaat gttttaaaaa cagctcggca
    12901 tctgccccaa tttgaaccgc caattcacgg gttggagcaa gaattaatgc tctcggctga
    12961 ttttttgacg tttcgatttc gttattcaat aaatggttaa aagttgcaat caaaaaggca
    13021 agcgttttcc ctgtacctgt ttgagcttgc cctgcaatat ctttgccttt cagcgtaatc
    13081 ggaaaggttt ttgcctgaat cggcgtacaa aattcaaagc ctttgctatc aagggcattt
    13141 agtacattag cgtgaatcgg gaagtcaata aagcggtgtt cggtaagatg attagacata
    13201 gtatttcctt gataattgtg atgtgacaag cggtcttttt tacacatttt tttgcaaaat
    13261 agcatattgg gcgttttttc tcaaatttaa ccgcttttga gtttattcgg caaaatagat
    13321 tggaaaagct ctcggctttt cagcggttgt gaccctaaat agggctttag tgccattcgg
    13381 gtaaaacgtt tcgctgcttg ttgagtactt ttttctgaaa aatccaactg agcaaacgct
    13441 aataaatcac tgcctaaaaa gctctggtta ttctgtaata atgaggcgat aaagccctga
    13501 ttttcctgaa attgataggt cattttcgga tcaactggca aaccggttgc cgcacagtgc
    13561 gtaaaatcca ccccataacc caatgctttt aacgtatgaa attcaaaggt gcgtaaaatc
    13621 ggctcaatct gtttttgtgt ggcaagctcg gtaatgcaac gtaaataatg ctgaaataat
    13681 tcagggtaag cggtctgatt ttccaatact cttgcaagta cctcgttgat ataaaaccca
    13741 ctataaagtg aaagcgtttc catcggcaac gttaatgcag ccggttctgc tttggttaaa
    13801 gttttcagat cccctttacc cgaccaacgc aacaacaacg gcgtgaacgg ctgtaaaacc
    13861 gctttcaacg gcgagcgagc ccgccttgca cctttagcaa gtaaagtaat ccgcccgtga
    13921 tgttcggtaa aaaactccac taataaactg ttttcgctgt attcacgccg atgaaggaca
    13981 aaggctcgtt gccattggtc tattgtcata atttcttgtt aattatgagg ttgtaattta
    14041 cttctgccaa atctccccct cccctctttg ctaaagaggg gagatcttgc agagtatatt
    14101 ttaaagtcat tttcactatt taacctttta tatagatagg aagatgaatt taaaatttct
    14161 ggagaactag tccccctctt tagaaaagag gggttagggg agatttgatt acgatattaa
    14221 tgctataacg ggagccaact actcgtccat ataccctaaa cttctcaatg ctctttcatc
    14281 atccgcccaa ccagctttta ctttcaccca aagctcaagg tgcacttggt tgtcaaatag
    14341 gcgttccata tcttttcgag cttcaatgcc gatggttttg attttttgcc cccccgcacc
    14401 aataaccatt tttttctgac catctcgttc tactaaaatc aagccattga tttcatacac
    14461 cccacgctca ttcattttaa attgttcgat ttctaccgtt accgaataag gtaactcttc
    14521 acctgtaaaa cgcattagtt tttcacgaat aatttctgat gccataaagc gttgagagcg
    14581 gtctgtcaca tactcttcag gaaagtggtg ctcaccttga cgcagtgatt gacggacaaa
    14641 tttctgtaaa atatgcacat ttttaccccg ttgtgccgaa attggcagaa tttcagcaaa
    14701 atcaaatttt tgtgaaagct cggtaatatg cggcagaagt tcttcttttt ccttgatgtt
    14761 atcaatttta ttgattgcca ataaaacagg ggcttttgcc gaacgcaatt tgtttaacac
    14821 catttcatca tcttctgtcc attttgtgcc ttccacaaca aagatgatga gatctacatc
    14881 gccaatagca gaacttgctg cacggttcat taaacgatta atcgcacgtt tttcttctat
    14941 gtgtaagcct ggggtatcta cataaatcgc ttggtattga tcttcagtgt gaataccaac
    15001 aatacgatgg cgagtcgttt gagctttgcg tgaggtaatg gaaatttttt gtcctaaaat
    15061 cttatttaat aatgttgatt tccctacatt tgggcgacca acaatcgcaa taaaaccaca
    15121 ataggttttt gcaggagttt gaatttgttc tgtcattttg ttttcctttc attctgtgga
    15181 atgaatttga tggtgcaagc gtccacgctt gtaccattta attaaatctt tagcacaagc
    15241 gaggacgctt gcgctatctt actttttcgt tttaaccacc gctaacgctt tttctgcggc
    15301 attttgttcg gcttttcggc ggctggtgcc aatgccgata aacacttcct cgacgttaat
    15361 cactttacag gttacacgga aagtttggtt atgagcttcg cctttaatat ctaatacatc
    15421 atattcaggc aatggcaatt ttctaccttg taaaaactct tgcaggcggg tttttggatc
    15481 tttttgtgaa tcgcctggac tgatatcttt aagcaaatct gaataccaat cttccacctt
    15541 ttccattgct tttgccatat cttgatcgag ataaatggcc gcaataatcg cttcaacaca
    15601 gtccgataaa atcgattctc ggcgaaaacc accacttttt agctctcctg gacctaattt
    15661 taggtagtcg cctaaatcaa atttgcgagc aataatcgct agagtttgct cacgcacaag
    15721 agttgctctc atacggctaa gctcaccttc attcgctttt ggaaatttgt cgtataatac
    15781 tttaccaata gtaaaattta aaattgaatc gcctagaaat tcaagacgct cattgttttt
    15841 ggttgccgca ctgcggtgtg ttaaggcctg aatgaggtaa tcaaggttat gaaattgata
    15901 acctaatttt ttctgtaatc gttctagttg catagtttat gtgaataaag gggcgtatct
    15961 catacgcccc tgttaagatt aattgattga ggtaaacatt ctagaaaaac gaagccccgt
    16021 tgggaattca tttgcttttt tatctaaact taaccaaata aaagtggctt taccaacgat
    16081 gtttctctca ggcacaaagc cccagaaacg actgtcttca ctgttatcac ggttatcacc
    16141 catcacaaaa tagtatcctt gtggcacaac ccattcacct tctgcttgcc cttcttgttg
    16201 gtagaaataa ggtgcatagt taaatgccat tgggtgattt aaaatttgat gagttacatt
    16261 acctttttca gtgcgttcta cctgcatttc accgtgatag aaaaactcag gatttggctt
    16321 gccttcgctg tattcaaata cctttgtttc gccattggct ttagtaaccg ttaattgctg
    16381 agttgcaaaa tcatatttaa ttttatcacc acctactgcc accacacgct taatataatc
    16441 aacgtgtggt tgtgccggtg ctttgaatac aatcacatcg cctcgttctg gtttgcccgt
    16501 ttcaatcaag gtgttttgcc agataggatc tttaatgccg tagctgaatt tatttacgac
    16561 taaaaaatcg cctacacgca aggttggctc cattgaacca cttggaattt gaaacggttc
    16621 aaataagaac gagcgaagta ctgtaacaaa gaataaaatg ccgaaaagtg aggcaaaaaa
    16681 ttcgcctact gcggatttag gttgaatttc cgctatttct tcagcggtta attctctgcc
    16741 taaacgtttt tgttgtagaa caatcgcctg tttacgtctt gggtcagcac tgaatttgta
    16801 aaacgcccaa aatccaccac aaataataac caaggcaact aaaataatgg aaatagtatt
    16861 tggcagttgc attgaatcaa gccattgcca aataccataa ataataccta gaaaaataat
    16921 tggcataaat tgtgccactg aatgctcctt tttattcaaa attgcaaagt tttctcaaaa
    16981 tatgaccgct tgcaagcggt cgtattttac acgaatattg acaattaatc cttacccaca
    17041 tgaagaattg ccaaaaatgc ttcttgtggc acttctacgt taccgagaga tttcattcgt
    17101 tttttaccct ctttttgttt ttgtagcagt ttcttcttac gacttacatc accaccgtaa
    17161 cattttgcta atacgttttt acgcaactgt tttaccgttg agcgagcaat gacgtggtta
    17221 ccaattgccg cttgaatagc gatgtcaaat tgttggcgtg gaataagttc acgcattttt
    17281 tccactaatt cacgtccacg atacgccgca tttgcacggt gaacgataat cgccaacgca
    17341 tcaacacgct cgccgttaat cataatatcc acacgcacca tatcagccgt ttggaaacgt
    17401 ttaaaaccgt aatcgagtga agcataacca cgagaggttg attttaagcg gtcgaagaag
    17461 tctaacacta cttcgcccat tgggatttcg taagttaagg cgatttggtt accgtgatag
    17521 accatattgg tctgtacgcc acgtttttcc acacaaaggg taatcacgtt gcctaggaat
    17581 tcttgtggca caagcatatt acattctgca atcggctcac gaatttctgc aatattgttg
    17641 attgctggga gtttcgatgg gctatccaca tagatcgttt cgccactggt ttgcaccact
    17701 tcgtaaacta cggtcggagc ggtagtaatc aaatcaagat cgtattcacg ctctaaacgc
    17761 tcttgaatga tctccatatg caacaagcct aagaagccac accggaagcc aaagcccaac
    17821 gcacttgaag tttctggctc atagaacaat gacgcatcat ttaggcttaa tttgcctaaa
    17881 gcatcacgga aggcttcgta gtcgtctgaa ctaatcggaa ataaccctgc ataaacctgt
    17941 ggttttactt tcttaaagcc cggtaatact tcagttgctg ggttgtggtg gtgggttaat
    18001 gtatcgccca ctggtgcccc taaaatatct ttaatcgcac aaactaccca acctacttca
    18061 cctgttttta gctcggtagt atccacttgt tttggcgtga aaataccaag gcgatctaca
    18121 ttgtaagact gccctgtact catcacttta attttatcgc cttttttcag cacgccattt
    18181 ttcacacgca ctaaagacac aacgcctaaa tagttatcga accaagagtc gataattaac
    18241 gcttgtaatg gtgcttccgc atcgccttct ggtgcaggca ttttcttcac gatttcttct
    18301 aaaacatctt caatgcctaa acctgtttta gctgaacagc gtactgcatc aatggcatca
    18361 atgccgacaa tatcttcaat ctcttctgcc acacgctcag gctcggctgc tggtaaatcg
    18421 attttattta aaattggcac aacctctaaa tctatctcaa ttgcggtata acaattcgcc
    18481 aacgtctgtg cttccacccc ctgccctgca tcaaccacta ataaagcacc ttcacaagca
    18541 gcaagcgagc gagaaacttc atatgagaaa tccacgtgcc ctggcgtatc aataaagttg
    18601 agctgataaa tttcgccatc tttcgcttta tagtttaagg ttacactttg tgctttaata
    18661 gtaattccac gctcacgctc aagatccatt gaatctaata cttgagcctc catttcacga
    18721 tctgataagc caccacaagt ttgaattaag cggtctgata aagtcgattt gccgtgatcg
    18781 atatgagcga taatcgaaaa gttacgaata ttttgcattt tcatcgggca gttaatgtcc
    18841 tgttttgtta tttgtttatc aaaaattaat cggggaattg tacttgaaat acaaggtttt
    18901 gcaaagaaaa caaatagcaa taagcggtta ttttttccta aaaatgtgca aatttcttga
//
LOCUS       DANLRK010000028        18609 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.028, whole genome shotgun sequence.
ACCESSION   DANLRK010000028 DANLRK010000000
VERSION     DANLRK010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 18609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18609
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.028"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            83..1501
                     /gene="leuC"
                     /locus_tag="PXA33_000726"
     CDS             83..1501
                     /gene="leuC"
                     /locus_tag="PXA33_000726"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HDL5120085.1"
                     /translation="MQQAKTLYQKLFDSHVVYEAEGETPIIYINRHLIHEVTSPQAFD
                     GLRVAGRQVRQVSKTFGTMDHSISTQVRDVQQLEGQAKIQVLELEKNCEETGIPLFDI
                     TKKQQGIVHVMGPEQGLTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTL
                     KQARAKSMKVEVRGKVNPGITAKDIVLAIIGKTTMAGGTGYVVEFCGEAIRDLSMEGR
                     MTVCNMAIEFGAKAGIVAPDETTFAYLKDRPNAPKGQDWEDAIEYWKTLKADEGAVYD
                     AEVILEAKDIAPQVTWGTNPGQVIGIDDVIPNPEDMADPVTRASAEKALAYIGLEAHT
                     DMKDVPVDQVFIGSCTNARIEDLRAAAAVMKGRKKADNVKRILVVPGSGLVKEQAEKE
                     GLDKIFIEAGAEWRNPGCSMCLGMNDDRLGEWERCASTSNRNFEGRQGRNGRTHLVSP
                     AMAAAAAVFGKFMDIRKVSLNA"
     gene            1514..2116
                     /gene="leuD"
                     /locus_tag="PXA33_000727"
     CDS             1514..2116
                     /gene="leuD"
                     /locus_tag="PXA33_000727"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HDL5120086.1"
                     /translation="MAGIKQHSGLVVPLDAANVDTDAIIPKQFLQAITRVGFGKHLFH
                     EWRYLDAEETQINPDFVLNFPQYQGATILLARKNLGCGSSREHAPWALADYGFKVMIA
                     PSFADIFYNNSLNNHMLPIKLTEEEVEEIFQWVWANEGKQIHVDLEAMTVTVGEKVYT
                     FELDEFRRHCLLNGLDNIGLTLQHEAAIAAYEKNIPAFLR"
     gene            complement(2188..2652)
                     /locus_tag="PXA33_000728"
     CDS             complement(2188..2652)
                     /locus_tag="PXA33_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5120087.1"
                     /translation="MNKFKIALMATVAIGLFACTPSPQSNSTKQQAQVGYLKQNISQA
                     ELNNPKDFKRYSYYCKNFATGSSSYLATYFPLWSESRKQENFGIYFQLDGGKVYPFDH
                     LANISLNARATKFEVRYRSYQPIEGSFVELVAREFTSTYYKNNVPWLECREG"
     gene            complement(2676..3182)
                     /locus_tag="PXA33_000729"
     CDS             complement(2676..3182)
                     /locus_tag="PXA33_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5120088.1"
                     /translation="MADPHIQSPMDFWDYLTVIIYRSGFVLATLMLLLLPYYPQTAQY
                     GLLVSGTMLASSLHLYAKSFRLIFQFSVWIGLLGAVFNFPLLAFGAMLLVIGGLSYKE
                     YFCFRVFGLNFQPLLVAVLWIAFALEWSLLVQILSLVCGLLLLVLSIQKWRMPLHFDI
                     GDKSKYQI"
     gene            complement(3205..4182)
                     /gene="dusA"
                     /locus_tag="PXA33_000730"
     CDS             complement(3205..4182)
                     /gene="dusA"
                     /locus_tag="PXA33_000730"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HDL5120089.1"
                     /translation="MTNEIQPFYRGRFSVAPMLDWTTRHCRYFHRQFSRQALLYTEMI
                     TAPAIIHAKYDLLEYDPSENPVALQLGGSDPAQLAHCAKLVAERGYAEINLNVGCPSD
                     RVQNGMFGACLMAKADLVADCIKAMQDAVEIPVTVKHRIGIDDLDSYEFLCDFIEKVQ
                     PYSNDFIVHARKAWLTGLSPKENREIPPLDYDRVYQLKRDFPHLKISINGGIKTIEEI
                     KQHLQFVDGVMVGREAYQNPSMLGEIDSQIFNENRPLVTAKEAVEKMFPYIERELAKG
                     VYLNHIVRHMLGAFQHCKGARQWRRHLSENATKSGAGVEVVEQALAFIG"
     gene            4533..5510
                     /locus_tag="PXA33_000731"
     CDS             4533..5510
                     /locus_tag="PXA33_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HDL5120090.1"
                     /translation="MSRNLFQKRLNIKPYEYPELLEFKDAIRHSYWLHTEFNFTGDIQ
                     DYRTTINDHERHVLTRAMLAISQVEVNVKRFWGELYCYFPKPEMDDVGGTFAESEVRH
                     KDAYSFLLEKLGLNEMFSQIHEIEPLMNRIRYMESFMKDKDAGKGEFVLSLVLFSLFV
                     EHISLFGQFIIMMSFNKHKNLFKGISNAVEATSKEEEIHGKFGIALYEILRDEHSELF
                     TPEFFEDLQTLANQAFEAERGILDWIFEEGDLSFITKATVENYIKNRYNNSLSILGLE
                     PPHDIDPELLKETEWFDIEILSTKETDFFNKRSTDYSKKMKQITADDLF"
     gene            5497..7164
                     /locus_tag="PXA33_000732"
     CDS             5497..7164
                     /locus_tag="PXA33_000732"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HDL5120091.1"
                     /translation="MTYFNTDRPDFHWLNEDSRLFLQRGYLLEGTTALDRIRFIAEHA
                     ERKLGIEGYADKFYHYMARGYFSLSSPIWSNFGLDRGLPISCFGSYIGDSIHEIMVTT
                     AEVGMMSKIGGGTSAYFGDIRPRGSLIKNNGKSDGSFNFSKLFDTVIDVISQGTSRKG
                     QFAGYIDIEHGDIDEWLDIHTEGNPIQLMYYGVCVGHDWLESMKAGDPYKRQLWAKLL
                     QRKTETGIPYLFFKDNANAGRPDVYKDKNMTIHASNLCTEIMLPSSNEESFVCCLSSM
                     NLLYFDEWKDTDAPETLTYFLDVVMSEFIEKSQSIPFLDRANRFARRHRALGLGVLGW
                     HSYLQANNIAFDSFQAMQKNNEIFKTLQAKTLKASQELAQRFGEPEILEGYGRRNTTL
                     MSIAPTKSSSFILGSVSPSVEPFKSNYYVKDLAKIKTVYKNPFLEKLLQEKGLDTEEI
                     WTSILQNDGSVQHLEQLSEHEKEVFKTFAEISQLSVIQQAAQRQKYIDQGQSINVMIH
                     PATPARDLNQLYLTAEELGLKSIYYQNSMSAAQVFNRNLLSCSSCEG"
     gene            7168..7587
                     /locus_tag="PXA33_000733"
     CDS             7168..7587
                     /locus_tag="PXA33_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5120092.1"
                     /translation="MGNLSHTSPNTIVEVVGEFPEITSSGYTFDKGPIQLRIGKHTAP
                     NKGFGVKHIEAEHMQEILNLGFTSVNEFVKAIIVENAKIFCEFSDLKGNHRPIILKAS
                     IGIVVLERKFLESGTIYSVVTAYRRTNPHGIQIGTMK"
     gene            complement(7822..8583)
                     /gene="nudC"
                     /locus_tag="PXA33_000734"
     CDS             complement(7822..8583)
                     /gene="nudC"
                     /locus_tag="PXA33_000734"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HDL5120093.1"
                     /translation="MNKGYWIIVSDFDIALEAGEIPFGSAEELGLSGREKLTIGQYQG
                     SPAYLVKALESDNQREFAYLRSQINRPPELSHLLHRAVSLNHFFQTHQFCGKCGEKTI
                     LASDEIAIYCNACGNRAYPTISPSIIVAVRKGRQILLANHLRHKGTIYTTLAGFVEAG
                     EAVETAVHREVFEETGIKVKNVRYFGSQPWAFPNSLMLGFLADYESGEIQLQEEEICD
                     AKWFDCEESLPEIPQQGTIAFELIQATLKLCRESK"
     gene            complement(8576..9259)
                     /locus_tag="PXA33_000735"
     CDS             complement(8576..9259)
                     /locus_tag="PXA33_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HDL5120094.1"
                     /translation="MKQPTFKRMTNKRSNFAKNLQKNPQKRPLTFAETTLVLLNKPYD
                     VLTQFTDEQGRKTLKDFITMPNIYPVGRLDRDSEGLLLLTNNGEIQHRLAHPKFDKQK
                     TYWVQVEGVPQPEDLAKLAKGVELKDGLTKPAKVRAIATPDFAWNIAPQIRERKTIPT
                     SWIELIISEGKNRQVRRMTAHIGFPTLRLIRVGIGQFRLGNLGCGEFQVLSENEKRKL
                     FTQLGLKDE"
     gene            complement(9256..10104)
                     /locus_tag="PXA33_000736"
     CDS             complement(9256..10104)
                     /locus_tag="PXA33_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDL5120095.1"
                     /translation="MEKRTLALLKVHGTAVLFGASGIFGALIQSSAETIVLGRVMIAF
                     AIISLYFLWKKQPLVKLNLQQMAQLAFSAALLAAHWVTFFVAVKVGGVAVATLGFASF
                     PAFVALFESLFFREKLRFREVVLLIAITVGLILVTPEFTFGNQATQGLLWGVLSALVY
                     GILAVANRRTMSKISGVQASWWQYIFATLFLLPLSITEFPSVSTQDWFWIFCIGFLCT
                     TLAYTLFISSLDTINARTASMIISLEPVYAIVIAWLCFNEVPTLKMIIGGLIILLSVA
                     WANLKK"
     gene            complement(10104..10589)
                     /locus_tag="PXA33_000737"
     CDS             complement(10104..10589)
                     /locus_tag="PXA33_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HDL5120096.1"
                     /translation="MPILAILFGIFFYIYCEISLLVNIGSIIGVLPTILLLVVISFLG
                     LWIVKLRGVYTLFSIKQDLSQGKIPTEAVGNSVMFIVAGILLIIPGFLSDILAILCVL
                     PFSRKWIQAFVINSVKNKVFMHFTAQTSSFSVTSAQDSNTFDAEFERKPDEQDQHNRI
                     K"
     gene            complement(10591..11913)
                     /gene="pepQ"
                     /locus_tag="PXA33_000738"
     CDS             complement(10591..11913)
                     /gene="pepQ"
                     /locus_tag="PXA33_000738"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HDL5120097.1"
                     /translation="MKNLFSTHLARLQQTVQSLLENAFLDGLWIYAGKAKTLFLDDQT
                     APFKINPYFNYFFPYPQAENCWLFLDGKNKPKIYFYAPEDYWVSTLKVPTEAFFAAEF
                     EWVLLRAENEIATFIQNPTACAFIGEDVELAKKLGFSHINPQKVLNSLNFARSIKTEF
                     EIESIYQAQFSALKGHQVAKEAFFAGKSEFEINLEYLKATQQSDLNVPYGNIVAINQN
                     GAVLHYTALDFDTHAKRHSFLIDAGTTVNGYASDLTRTYAFDSGSEFAELVAKMEQLK
                     LQIIAELSIGVNYLHYHTRMQQMISQLLAEHQFIHLSAEQIFEQGISRTFFPHGLGHL
                     LGIQVHDVGGFQQNARGSHKAPPEVYPSLRCTRELQENMVLTIEPGFYFIEMLLKPWK
                     NSALSSAFNWQKIEAFKAFGGIRTEDNIVMRANGAENLTANAAATLGG"
     gene            11924..12166
                     /gene="tusA"
                     /locus_tag="PXA33_000739"
     CDS             11924..12166
                     /gene="tusA"
                     /locus_tag="PXA33_000739"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005710491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HDL5120098.1"
                     /translation="MTQLTVNKELDTLGLRCPEPVMLTRKTIRNMEEGEILHIIADDP
                     ATTRDIPSFCEFMDHQLIQKEVDSTPYHYWIKKGLE"
     gene            complement(12233..12664)
                     /gene="rraB"
                     /locus_tag="PXA33_000740"
     CDS             complement(12233..12664)
                     /gene="rraB"
                     /locus_tag="PXA33_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HDL5120099.1"
                     /translation="MYNLDELRTETDETISSLLADGSDPEALYIIEHHVAHHDFDKLE
                     KLVVDAYKLGYEISEAEEVEDENGKVIFVCDIVSEIHLNADLITAQQKELLPLIEKAG
                     AEYEGWGTYFEDPNADEDEYGDDGEFFDDENDDFEDEPRRH"
     gene            complement(12679..13290)
                     /locus_tag="PXA33_000741"
     CDS             complement(12679..13290)
                     /locus_tag="PXA33_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HDL5120100.1"
                     /translation="MKLNYHKTHFLTSAPDIRHLPEDTGVEIAFAGRSNAGKSTALNA
                     LTNQKSLARTSKTPGRTQLINLFKVETNCKLVDLPGYGYAAVPEQMKIQWQKALGEYL
                     QKRECLGGVVILMDIRHPLKDLDQQMIEWAVSANLPVLLLLTKADKLSQSARSKQVKM
                     VREAILPFQGDIQVEAYSALNKIGIDKLSNKLDSWFAPKLITE"
     gene            complement(13292..13708)
                     /locus_tag="PXA33_000742"
     CDS             complement(13292..13708)
                     /locus_tag="PXA33_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HDL5120101.1"
                     /translation="MSWYKALFSFNGRLNRRGFWIGFGIHFLFLFMVAMWINLAELSL
                     LSVLPLIITGFSLSAIIIKRLHDRNRSAKALLMALVPIICYLAAPYSQGIMQILLGQV
                     MPAFIGMLLFLEWGVFAGNPEPNQYGEQGLSFKFRE"
     gene            complement(13712..14542)
                     /locus_tag="PXA33_000743"
     CDS             complement(13712..14542)
                     /locus_tag="PXA33_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HDL5120102.1"
                     /translation="MFEYFLSLSPIWQAFLAGLFTWGCTIFGSSFVYFFKSVNRKLLD
                     SMMGFAAGVMIAASFWSLLAPSLEYAEADYGSLAWLPAAIGFLAGGAFIRLIDYVVPH
                     LHLSKPIEQAEGNDQLKQGLSKSTLLFLAITIHNIPEGLAIGVAFGALATQVSGVDAS
                     IMGAIGLAIGIGLQNIPEGSSLVLPIRGEGHSRKKAFWYGSMSAVVEPIAAVIGAVFV
                     LSMTAILPYALAFAAGAMIFVVVEELIPESQSSGNTDIATLSLMLGFVVMMVLDVALG
                     "
     gene            complement(14879..14994)
                     /gene="rrf"
                     /locus_tag="PXA33_000744"
     rRNA            complement(14879..14994)
                     /gene="rrf"
                     /locus_tag="PXA33_000744"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(15156..18054)
                     /locus_tag="PXA33_000745"
     rRNA            complement(15156..18054)
                     /locus_tag="PXA33_000745"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            complement(18371..18446)
                     /locus_tag="PXA33_000746"
     tRNA            complement(18371..18446)
                     /locus_tag="PXA33_000746"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18411..18413),aa:Ala,seq:tgc)
     gene            complement(18503..18579)
                     /locus_tag="PXA33_000747"
     tRNA            complement(18503..18579)
                     /locus_tag="PXA33_000747"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18543..18545),aa:Ile,seq:gat)
BASE COUNT         5478 a         4296 c         3616 g         5219 t
ORIGIN      
        1 tcagcaagcg gtcatttttg caaaattttt tgcaaattta accgcttgta ttaaaagtta
       61 cccttttgag ttggaacatt ttatgcaaca agccaaaacc ctatatcaaa aactattcga
      121 ctcacacgtt gtgtatgaag ccgaaggcga aacgccgatt atttatatta accgccactt
      181 aattcacgaa gttaccagcc cgcaagcgtt tgacggcttg cgtgttgccg gtcgccaagt
      241 tcgccaagtc agtaaaacct tcggcacaat ggaccacagt atttcaaccc aagtgcgtga
      301 tgtacagcaa cttgaagggc aagcgaaaat tcaagtatta gaattagaaa aaaactgcga
      361 agaaaccggt atcccactgt ttgacatcac taaaaaacaa caaggtatcg ttcacgtaat
      421 ggggccggag caaggcttaa ctttacccgg tatgaccatt gtatgtggtg actcgcacac
      481 cgccactcac ggggcctttg gtgcgttagc cttcggtatc ggtacttccg aagtggaaca
      541 cgttttagcc acccaaacct taaaacaggc ccgtgcaaaa agtatgaaag tggaagtccg
      601 tggcaaagta aacccgggca ttaccgcaaa agatattgtg cttgcgatca tcggtaaaac
      661 aactatggct ggcggtacag gctatgtagt ggaattttgt ggcgaagcaa ttcgtgatct
      721 ttcaatggaa gggcgaatga cagtgtgtaa tatggcgatt gagtttggtg cgaaagccgg
      781 tattgttgcc ccggatgaaa ccacgttcgc ttacttgaaa gaccgcccaa atgcaccaaa
      841 aggtcaagat tgggaagatg ccattgaata ttggaaaacc ctcaaagccg atgagggtgc
      901 agtttacgat gcggaagtga tcttagaagc aaaagacatt gctccacaag tgacttgggg
      961 aaccaacccg gggcaggtga tcggtattga tgatgtaatt ccaaatccgg aagatatggc
     1021 cgatcctgtt actcgtgcct ctgcggaaaa agccttagct tacatcggct tagaagccca
     1081 tacagatatg aaagatgtac cggttgatca agtctttatt ggttcttgta ctaatgctcg
     1141 tattgaagat ttgcgtgctg cggcagcagt aatgaaaggg cgtaaaaaag ccgacaatgt
     1201 aaaacgtatt ctcgttgtgc caggttcagg tttggtaaaa gagcaagcag aaaaagaggg
     1261 cttggataaa atcttcattg aagccggtgc agaatggcgt aacccgggtt gctcaatgtg
     1321 tttgggaatg aacgatgacc gcttgggcga atgggaacgt tgtgcctcta cgtctaaccg
     1381 taactttgag ggcagacaag gacgtaacgg tcgtacccac ttagtcagcc cggcaatggc
     1441 ggctgcggcg gcggtgttcg gtaaatttat ggatattcgc aaagtgtcat taaacgcata
     1501 ataggaggaa aaaatggcag gtattaaaca acattcaggc ttggtcgttc ctcttgatgc
     1561 agcgaacgta gacaccgatg caatcattcc aaaacaattt ttgcaagcca ttactcgagt
     1621 cggctttggt aagcaccttt tccacgaatg gcgttattta gatgcggaag agactcaaat
     1681 caaccccgat ttcgtactga acttcccaca atatcaaggt gcaaccattt tactcgcccg
     1741 taaaaattta ggctgtggct cttctcgtga gcacgcccct tgggcattgg cagattacgg
     1801 ttttaaagta atgatcgccc caagttttgc ggatattttc tacaataaca gcttaaataa
     1861 ccatatgttg ccgattaaat taaccgaaga ggaagtcgaa gagatcttcc aatgggtgtg
     1921 ggcaaatgaa ggaaaacaga tccacgttga tttagaagcg atgactgtga ctgttggcga
     1981 gaaagtttat acctttgagt tagacgaatt ccgccgccac tgcttattaa acggtttgga
     2041 taatatcggc ttaaccctac aacacgaagc tgcgattgct gcttatgaga aaaacattcc
     2101 ggcattttta cgctaattaa aatacaaaaa aacggctaac atctttaagg cgttagtcgt
     2161 tttcttttta gccattttct ttggcaatta gccttctcgg cactctaacc aaggcacatt
     2221 atttttgtaa taagttgaag taaactctcg tgccactaac tctacaaaac tgccttcaat
     2281 cggctgataa gaacgataac gcacttcaaa ttttgttgct cttgcattca aactgatgtt
     2341 ggcaagatgg tcgaacggat aaacctttcc gccatcaagt tgaaaataga taccaaaatt
     2401 ctcttgctta cggctttcag accaaagtgg aaaataggtt gccaaatatg aactgctgcc
     2461 tgttgcaaaa ttcttacaat aatagctata acgcttaaaa tctttcggat tattcaattc
     2521 cgcttggctg atattctgtt tcaaatatcc aacctgtgct tgttgcttag ttgagtttga
     2581 ttgtggtgag ggcgtacaag caaataagcc aattgccact gttgccatta aagcaatttt
     2641 gaatttattc ataaaaatcc cgataaaata ttaaattaaa tctgatattt gcttttatcg
     2701 ccaatatcaa aatgcagcgg cattcgccat ttttgaatac tcagcacaag aagcaacaag
     2761 ccacagacaa ggcttaaaat ttgcaccaat aaagaccatt ctagtgcaaa agcaatccac
     2821 aataccgcca ctaaaagcgg ttggaaattt aagccgaata ctctaaaaca gaaatattct
     2881 ttatagctta atccgccgat caccagtagc attgccccga acgcaagcaa agggaaatta
     2941 aacacagcac caagtaagcc aatccaaaca gaaaattgga aaatcaggcg aaacgatttg
     3001 gcataaagat gcaaagaaga tgccaacatt gtgccagata ctaataaacc atattgtgcc
     3061 gtttgcggat aataaggcaa taaaagcaac atcaatgtag ctaacacaaa accgctgcgg
     3121 taaatgatga cggttaaata atcccaaaaa tccattggcg attgaatatg tggatctgcc
     3181 ataactgcct ccttaaacaa taaattagcc aataaacgca agggcttgtt ccactacttc
     3241 tacaccggct cccgacttag tggcattttc acttaaatga cgacgccact gcctcgctcc
     3301 tttgcaatgt tgaaacgcac caagcatatg gcgaacgata tgatttaaat agacaccttt
     3361 ggcaagctca cgttcaatat aagggaacat cttttccacc gcttcttttg ccgttacaag
     3421 cggtcgattt tcattaaaaa tttgcgaatc gatctcgcct agcattgacg ggttttgata
     3481 ggcctcacgc cctaccatta ccccatcaac aaactgcaaa tgctgtttaa tttcctcaat
     3541 ggttttaatg ccgccattga tgctaatctt taaatgcgga aaatctcgct tgagctgata
     3601 aacccgatca taatctaaag gcggaatttc acggttttcc ttcgggctta accctgtcag
     3661 ccacgccttg cgggcgtgta cgataaaatc attgctataa ggttgcactt tttcgataaa
     3721 atcacacaaa aattcatagc tgtctaaatc atcaatccca atacgatgtt taaccgtgac
     3781 cggaatttcc accgcatctt gcattgcttt aatacaatcc gccactaaat ctgcttttgc
     3841 catcaaacaa gccccaaaca taccgttttg cactcggtct gacgggcaac cgacatttag
     3901 attaatctcg gcatagcctc gctctgccac caatttggca caatgagcca actgtgccgg
     3961 gtcgcttcca cccagttgca aagccaccgg attctcgctc ggatcatatt ccaacaaatc
     4021 atatttggcg tgaataatcg ccggggcggt gatcatttcg gtataaagca aggcttgacg
     4081 actaaattgg cgatgaaaat agcggcagtg acgggttgtc caatctaaca ttggagcaac
     4141 cgaaaaacgt ccacggtaaa aaggttgaat ttcgttagtc ataaaaatat caaaataaag
     4201 cggtaaaaaa tcagtaaaaa ataaccgctt gtaagcgatt caaaacaagc agccatcata
     4261 ctcgaaaaag ccagataaac atagtttcct caaggcttcc ccatctatct gcgcactttt
     4321 aaaacaacct taaaacacaa tatatagtgt ttcaacatca aatcacacac aacatattga
     4381 agcaaaaatt tacaaaatta tttttcttta taaatcaata agctaccaaa gaccaataaa
     4441 aaagagtgtt tgtcaagact tgtcttgaaa ctttttttgc atacaatcat ctaccagttc
     4501 caaacataaa ctttaagata gagcctacga atatgtctag aaacctcttc caaaaacgct
     4561 taaatattaa gccgtatgag tatcctgagc ttcttgaatt taaagatgca attcgccatt
     4621 cttactggtt acacaccgaa tttaacttca ccggcgatat tcaagactat cgcaccacca
     4681 ttaacgatca cgaacgtcac gtgttaaccc gagcaatgct tgcgatttca caagtagaag
     4741 tgaatgtaaa acgtttctgg ggcgagctat actgctattt cccaaaaccg gagatggacg
     4801 atgtaggcgg tacttttgct gaaagtgaag ttcgccataa agatgcttac tcgttcctat
     4861 tggaaaaatt aggcctaaat gaaatgttca gtcaaattca tgaaattgaa ccattaatga
     4921 accgcattcg ttatatggaa agttttatga aagataaaga tgctggcaaa ggcgaatttg
     4981 ttttatcgtt agtgctgttc tctttatttg ttgagcatat ttcattgttt ggacagttta
     5041 ttatcatgat gtcgttcaac aagcataaaa acctgtttaa agggatttcc aatgcggttg
     5101 aagccacctc aaaagaggaa gaaattcacg gtaaattcgg aattgcgtta tacgaaattt
     5161 tacgtgacga acacagtgaa ctattcacac cagaattttt tgaagatttg caaaccttgg
     5221 caaatcaagc gtttgaagca gaacgtggca tcttagactg gattttcgaa gaaggtgatt
     5281 taagctttat caccaaagcg acggtagaaa attacattaa aaaccgttac aacaattctt
     5341 taagcatttt agggttagag ccgccacacg acatcgaccc tgaattatta aaagagactg
     5401 agtggtttga tattgaaatt ctttcaacca aagaaaccga tttctttaac aaacgtagca
     5461 ccgattattc taaaaaaatg aaacagatta ccgcagatga cttattttaa tacagaccgc
     5521 ccggacttcc actggcttaa tgaagacagc cgtttattct tacaacgtgg ctatttattg
     5581 gaaggaacaa ccgcattaga ccgcattcgt tttattgccg aacacgcaga gcgaaaactc
     5641 ggcattgaag gctatgccga taaattctat cactatatgg caagaggcta cttctcgctt
     5701 tcttcgccaa tttggtcaaa ctttggctta gatcgtggct taccgatttc ttgtttcggt
     5761 agctacatcg gcgactctat ccatgaaatt atggtgacta ccgctgaagt gggcatgatg
     5821 agtaaaattg gcggtggtac ttcggcctac tttggcgata ttcgtccgcg tggctcgtta
     5881 attaaaaaca acggtaaatc agacggctca tttaacttca gtaaattatt tgacactgtg
     5941 attgacgtga tttcacaagg aacatcacgc aaaggtcagt ttgccggtta tattgatatt
     6001 gagcacggcg atattgacga atggctggat attcacaccg aaggcaaccc aattcaatta
     6061 atgtattacg gtgtgtgtgt cggtcacgat tggttagagt caatgaaagc cggcgatcca
     6121 tacaaacgtc agctttgggc aaaattgtta caacgtaaaa ccgaaaccgg tatcccttat
     6181 ttattcttta aagacaatgc caacgcaggt cgtcctgatg tatataaaga taaaaatatg
     6241 acgattcacg cctctaactt atgtactgaa attatgttgc cttccagcaa cgaagaaagt
     6301 ttcgtatgct gtttatcgtc tatgaaccta ctctactttg atgagtggaa agataccgat
     6361 gcaccagaaa cattaaccta tttcctcgat gttgtaatga gtgaatttat tgagaaaagt
     6421 caatcaattc cattcttaga ccgtgcaaac cgctttgccc gtcgccatcg tgcattaggt
     6481 ttaggcgtgt tgggctggca cagctatcta caggcaaata atattgcctt tgacagcttc
     6541 caagcgatgc aaaaaaacaa cgagattttc aaaacgcttc aagcaaaaac cttgaaagcc
     6601 tcgcaagaat tggctcaacg cttcggcgag ccggaaattt tagagggcta tggtcgccgt
     6661 aatactacat taatgagtat tgctccgact aaatccagta gctttatttt aggcagtgtg
     6721 tcgccatcgg tagagccgtt taaatccaac tactatgtaa aagatttagc gaagatcaaa
     6781 accgtgtata aaaacccgtt cttagagaaa ttactccaag aaaaagggct agacaccgaa
     6841 gaaatttgga cgtctatttt acaaaatgat ggctcggttc agcatttgga acagctttcc
     6901 gagcacgaaa aagaagtatt taaaaccttt gccgaaatca gccaattaag cgtgattcag
     6961 caagctgctc aacgtcaaaa atatattgac caagggcaaa gtattaatgt gatgatccac
     7021 ccagccacac cggctcgtga tcttaaccaa ttatacttaa cagcagaaga gctgggctta
     7081 aaatccatct actaccaaaa ctcaatgagt gcagcacagg tatttaaccg taacctatta
     7141 agctgctcaa gttgcgaagg gtaaataatg ggaaatttat cgcatacttc acctaatact
     7201 attgttgaag ttgttggtga atttcctgaa ataacatcta gtgggtatac ctttgataaa
     7261 ggtcctattc aattaagaat cggcaaacat actgctccaa ataaaggctt tggtgtaaag
     7321 catattgaag ccgagcatat gcaagaaatt cttaatctag gatttacatc agttaatgaa
     7381 tttgtcaaag ctatcatagt agaaaacgct aaaatttttt gcgaattctc agatttaaaa
     7441 ggcaatcatc gccctattat tctgaaagca agtattggca ttgtggttct agagcgtaag
     7501 tttttggaaa gcggtacgat atattcagtt gtaaccgctt atagaagaac aaatcctcac
     7561 ggaatacaaa taggcactat gaaataaagt acgttagtgt tataatcagt tccacgcaag
     7621 agcatttgta gaaaataaca ggcggatttg gttttctact gtgggcggca ggtttccgct
     7681 aacctgtaat agctatttaa tttttatatt aaatcctgaa cttgaaacag actaagggat
     7741 tgctgcggta gctaagaata caaacctctc tataatcata tagagaggtt ttttcttatt
     7801 gatgagcaca agttaagata attatttact ctcccgacat aacttcaagg tagcttgaat
     7861 cagctcaaaa gctattgtgc cttgttgtgg gatttcaggt aagctttcct cacagtcgaa
     7921 ccattttgca tcgcagattt cttcttcctg tagctgaatt tcaccgcttt cataatccgc
     7981 taaaaaacct agcattaatg aattgggaaa cgcccaaggt tggcttccaa aataacgcac
     8041 atttttcact ttaatgcccg tttcttcaaa cacttcacga tgcaccgcag tttctaccgc
     8101 ctcaccggct tcgacaaaac cggctaaagt ggtataaatt gtgcctttat ggcgtagatg
     8161 attggctagt aggatttgtc tgccttttcg caccgccaca ataattgacg ggctaatcgt
     8221 tgggtaagct cgattgccac acgcattgca gtaaattgca atttcatcgc ttgccagtat
     8281 ggttttctcg ccacattttc cgcaaaattg gtgagtctga aagaaatggt taagcgaaac
     8341 agctcgatgc aacaaatgag acagttcggg tggtcggtta atttggcttc ttaaataagc
     8401 aaactctcgc tgattatccg attctaacgc cttcaccaaa taagccggcg agccttgata
     8461 ttgcccgatg gttaatttct cacgtccact taagcccagc tcttccgcac ttccgaatgg
     8521 aatttcccca gcctccaagg caatatcaaa atcgctgacg ataatccaat atcctttatt
     8581 catctttcaa tcctaattgt gtaaacaact tacgtttttc attttcagat agcacttgaa
     8641 actcaccaca gcctaaattg cctaatctaa actggcctat cccaacacga attaaccgta
     8701 aagtcgggaa accgatatgt gcagtcattc ttctgacttg gcgatttttc ccttcagaaa
     8761 taatcaattc tatccagctg gtcggaatgg ttttccgctc acgaatttgc ggggcgatat
     8821 tccaagcaaa atcgggcgtt gctattgctc tgactttcgc gggcttggtc aaaccatctt
     8881 ttaactccac cccttttgct aatttggcaa gatcttccgg ttgtggaaca ccctccactt
     8941 gtacccaata ggttttctgc ttgtcaaatt tcgggtgagc taaacggtgc tggatttcgc
     9001 cattattagt caaaagcaac aatccttcac tgtctctatc tagccgcccg accgggtaaa
     9061 tattgggcat cgtaataaaa tcttttagcg tttttcgccc ctgttcatcg gtaaattggg
     9121 ttagtacatc ataaggctta ttgagtaaca caagggtggt ttccgcaaag gtaagcggtc
     9181 gtttttgcgg atttttttgc aaatttttag cgaaatttga ccgcttgttt gtcattcttt
     9241 taaaggttgg ttgtttcatt ttttcaggtt cgcccacgca acagaaagta aaataattaa
     9301 gccaccgata atcattttaa gggttggcac ttcattaaag caaagccacg cgatcacaat
     9361 cgcatacacc ggctcgaggg aaataatcat tgaggcagta cgggcgttga tggtatcaag
     9421 gcttgaaata aacagcgtat aggcaagggt tgtacataaa aaaccgatac agaaaatcca
     9481 aaaccaatct tgggtggaaa cgcttggaaa ttcagtgatg gagagaggca acagaaaaag
     9541 tgtagcaaaa atatactgcc accagcttgc ttgcacaccg gaaattttgc tcatggtacg
     9601 gcgattcgct accgccaaaa taccatacac caaagcagat aacacgcccc acaataaacc
     9661 ttgagtggct tgattaccaa aagtaaattc aggggtaact aaaattaaac caaccgtgat
     9721 ggcaattaat agcaccactt cacgaaagcg taatttttca cggaagaaca ggctttcaaa
     9781 taacgccaca aatgccggaa agctggcaaa gcctaaggtt gctaccgcca caccacctac
     9841 tttaaccgcc acaaaaaaag taacccagtg ggcagctaat aaagctgcag aaaatgcaag
     9901 ttgagccatt tgctgtaagt tgagctttac aagcggttgt tttttccata aaaaatacaa
     9961 agagatgata gcaaaagcaa tcatcactcg cccaagcaca atggtttcag ccgagctttg
    10021 aattaatgct ccgaagatcc ccgaggcacc aaataaaacc gctgtgccgt gtactttaag
    10081 tagtgcaagt gttcttttct ccactatttg atcctattat gttgatcttg ctcgtccggc
    10141 ttgcgttcaa attctgcatc aaacgtgttg ctatcttgcg cagatgtaac agaaaaagac
    10201 gaagtttggg cggtaaaatg cataaacacc ttattcttta cgctattaat tacgaaggct
    10261 tgaatccatt ttcggctaaa gggtaacacg cataaaatcg ctaaaatatc acttaaaaag
    10321 ccgggaataa tcagcaaaat gccggctaca ataaacatca ccgagttgcc gactgcttca
    10381 gtcggtatct tgccttggct taaatcttgc ttgatggaaa agagggtata aacgcccctt
    10441 aacttcacaa tccataaccc taaaaatgaa atcacgacca gtaacaaaat cgtgggtaac
    10501 acgccaatga tcgagccaat attcaccagc agcgaaattt cgcaatagat ataaaagaaa
    10561 ataccaaaca gaatcgctaa aataggcata ttatcctccc aacgttgcgg cagcgtttgc
    10621 cgtcagattt tctgccccat tggctcgcat gacaatattg tcttcggtac gaatcccgcc
    10681 aaaggctttg aaagcctcaa ttttctgcca gttaaaggca gaagataagg cactgttttt
    10741 ccaaggctta agtagcatct caataaaata aaaacccggc tcaatggtaa gcaccatatt
    10801 ttcctgtagc tcacgggtac aacgcaaact tgggtaaacc tctggaggag ctttatggct
    10861 gcctcgggca ttttgctgaa aaccgcccac atcgtgcact tgaataccca ataaatgccc
    10921 tagtccgtgt gggaagaacg tacggctaat cccttgttca aaaatttgct cggctgaaag
    10981 atgaataaat tgatgttccg ctaataattg cgaaatcatc tgctgcattc gggtgtgata
    11041 atgcaggtag ttcaccccaa tactcagctc agcaataatc tgcaacttca actgttccat
    11101 tttcgccacc agctcggcaa attcagaacc gctgtcaaag gcataggtgc gggtgagatc
    11161 ggaggcataa ccatttaccg ttgtgccggc atcaattaaa aaactgtggc gttttgcgtg
    11221 ggtgtcaaaa tctaacgccg tataatgtag caccgcacca ttttgattaa tcgccacaat
    11281 attgccataa ggaacattga gatcagattg ctgcgtcgct ttcaaatatt caaggttgat
    11341 ttcaaattcg cttttgccgg caaaaaatgc ctcttttgct acttgatgcc cttttaaggc
    11401 tgaaaattgg gcttggtaaa tactttcaat ttcaaactcc gttttgatgg agcgggcaaa
    11461 attcaggcta ttcagcactt tctgcggatt aatatggcta aagcctaact ttttggctag
    11521 ctcaacatct tcaccgataa aggcacaagc ggtcggattt tggataaagg ttgcaatttc
    11581 attctctgct ctcaacaaaa cccactcaaa ttccgcagcg aaaaaggcct ccgttggcac
    11641 ttttagagtg gaaacccaat aatcttcagg ggcataaaaa tagattttcg gcttattttt
    11701 gccatctaaa aataaccagc aattttcagc ttggggatat gggaaaaagt aattaaaata
    11761 agggttaatt ttaaacgggg cggtttgatc gtctaaaaat aaggtttttg ccttgccggc
    11821 ataaatccat aaaccgtcta aaaaggcatt ttccagcaag ctttgcactg tctgttgcaa
    11881 acgtgcgaga tgcgtagaga atagattttt cataaggaat aaaatgactc aattaactgt
    11941 aaataaagaa ttagatacct tggggctacg ttgcccagag ccggtgatgc tcacccgtaa
    12001 aacaatccgt aatatggaag agggagaaat tttgcatatc atcgccgatg acccggccac
    12061 tacccgtgat attccgagtt tctgcgaatt tatggatcac caattaattc aaaaagaggt
    12121 ggactccacc ccataccatt actggattaa aaaaggcttg gaataaacca caagcggtcg
    12181 attttagcca taaaattgca aatttatgca caaaatcgac cgcttgtttt aattaatgac
    12241 ggcgaggttc gtcctcaaaa tcgtcatttt catcatcgaa aaattcaccg tcatcgccgt
    12301 actcatcttc atcagcattc ggatcttcaa agtaggttcc ccagccctca tactccgcac
    12361 cggctttttc aattaatggc agtaactctt tttgttgtgc ggtaatgaga tccgcattca
    12421 agtgaatttc gctcacaatg tcgcatacaa aaatcacttt accattttcg tcttccactt
    12481 cttccgcttc ggaaatttca tagcccagct tataggcatc aactactaat ttttccaatt
    12541 tatcaaaatc gtggtgagca acgtggtgtt caataatata aagggcttca gggtctgaac
    12601 catcggctaa taatgatgaa atggtttcat cggtttctgt gcgtaattca tcgagattgt
    12661 acatattgaa ttccttactt attctgtgat taattttggt gcaaaccagc tatctaattt
    12721 gttcgacagc ttatcaatac cgattttatt cagtgcggaa taagcctcta cttggatatc
    12781 accttggaat ggtaaaatcg cttctcttac catttttacc tgcttagagc gtgccgattg
    12841 gcttaactta tccgctttgg tcaacagcag taacaccggc aagttggctg acactgccca
    12901 ttcaatcatc tgttgatcta aatctttcaa cgggtggcga atatccatta aaatcaccac
    12961 accgcctaaa cattcacgct tttgtaaata ctcgcctaat gctttttgcc actggatttt
    13021 catctgttca ggtactgctg catagccata gccaggtaaa tccactaatt tacaattcgt
    13081 ttcaacctta aacaagttaa tcaactgcgt tcgccccggt gttttggaag ttcgggctaa
    13141 acttttttgg ttagttaaag cgttcaaagc agtcgattta ccggcatttg aacgcccggc
    13201 aaaagcaatc tccacacctg tatcttcagg taaatgacga atatccggtg ctgaagttaa
    13261 aaaatgggtt ttatgatagt ttaatttcat tttattctct aaatttaaaa gataagcctt
    13321 gctcgccata ttgattcggt tcaggattac cggcaaatac accccattct aaaaagagca
    13381 acattccaat aaatgccggc atcacctgtc caagcaatat ctgcataatt ccttgtgaat
    13441 aaggagctgc aagataacaa ataatcggca ctaatgccat taacagggct tttgccgaac
    13501 gatttcggtc gtgtaatcgt ttaataataa tagcagacag gctaaatcct gtaattataa
    13561 gcggtagcac actcaataaa gacaattcag ccaaatttat ccacatagca accataaaca
    13621 ggaaaagaaa gtggattcca aatccaatcc aaaaacctcg tctatttagc ctaccgttaa
    13681 aactaaaaag tgctttatac caactcattt tctaccctaa agctacatca agtaccatca
    13741 ttactacaaa gcctaacatc aggcttaaag tcgcaatatc agtatttccc gaactttgcg
    13801 attccggaat cagttcttct acgaccacaa aaatcatcgc tccggcagca aaggcaagag
    13861 cataaggtaa aatagccgtc atcgacagca caaaaaccgc cccaatcacc gccgcaatag
    13921 gctcaaccac tgctgacata gagccatacc aaaatgcttt cttccgacta tgcccttcgc
    13981 ctcgaattgg caatacaagt gacgagcctt cgggaatgtt ttgtaaccct attccgattg
    14041 ccaaaccgat agctcccata atggaagcat ctacgcctga aacttgggtt gccaaagcac
    14101 cgaaagccac ccctatggct aatccttctg gaatattatg aatggtaatc gcaagaaaaa
    14161 gcaaagtaga ttttgataag ccttgtttaa gttgatcatt tccttcagct tgctcaatcg
    14221 gcttactcaa gtgcaaatgc ggcacaacat agtcaattaa tcgaataaaa gctccaccgg
    14281 caagaaagcc aatcgctgca ggcagccaag ctaaagagcc ataatccgcc tcggcatatt
    14341 ctaaagaagg tgcaagcaaa gaccaaaacg aggctgcaat cattacacct gcggcaaagc
    14401 ccatcatact gtctaataat tttcgattaa ccgatttaaa aaaatagaca aatgacgaac
    14461 cgaaaatcgt acaaccccaa gtaaataaac ccgctaaaaa agcttgccat attggagaaa
    14521 gtgaaaggaa atattcaaac aagaaatagt accagctaaa ataaatattg gctcattcta
    14581 tcaaagattc agggttatta atatagtgta gccaaaggct caacgaagat ttcacttgta
    14641 aaacttagca agaaaataac cgcttgcatt tccctttcat aaatacaatg aaaatcctaa
    14701 caagcggtca ttttccgtga gttttttacc tttaccacaa caacaaaaaa ccgcctgtat
    14761 ctctacaggc ggctctttct tggattactt gtactttgtt tatttgtact tattttgctt
    14821 ttgcttaagt tttcgctttt gtaactttta gctttcgctt aagctcgctt aaattaaaac
    14881 ccgacgatgc tctactctca catggcgaat caccacacta ccatcgacgt tactgcgttt
    14941 tacttctgag ttcggtatgg ggtcaggtag gaccacagca ctcttgtcgt cgggaaaatt
    15001 cttttctatt tttgaatttc tttaagttaa cttcctaatc taactcttag ttcactttaa
    15061 tagttagatt aacttcctta acttcgattg atttcttctc tagtttcgtt cttcttctca
    15121 atacaaaaac aagcctaaaa acatttgagc gttgtatagt taagcctctc gggcaattag
    15181 tactggttag ctcaatgtct cacaacactt acacacccag cctatctacg tcgtagtctc
    15241 caacaaccct tacacactta tagtgtggga gaactcatct cgaggcaagt ttcgtgctta
    15301 gatgctttca gcacttatct cttccgcatg tagctaccca gcaatgcctc tggcgagaca
    15361 actggaacac cagtgatgcg tccactccgg tcctctcgta ctaggagcag cccctctcaa
    15421 ttctccaacg cccacggcag atagggaccg aactgtctca cgacgttcta aacccagctc
    15481 gcgtaccact ttaaatggcg aacagccata cccttgggac ctacttcagc cccaggatgt
    15541 gatgagccga catcgaggtg ccaaacaccg ccgtcgatat gaactcttgg gcggtatcag
    15601 cctgttatcc ccggagtacc ttttatccgt tgagcgatgg cccttccatt cagaaccacc
    15661 ggatcactat gacctgcttt cgcacctgct cgacttgtct gtctcgcagt taagcttgct
    15721 tataccattg cactaacctc acgatgtccg accgtgatta gcaaaccttc gtgctcctcc
    15781 gttactcttt gggaggagac cgccccagtc aaactaccca ccagacactg tccgagaccg
    15841 cgttccgcaa tcttcgttag aacatcaaac gttaaagggt ggtatttcaa ggacgcctcc
    15901 acaatcactg gcgtgactgc ttcaaaggct cccacctatc ctacacatca aaattcaatg
    15961 ttcagtgtca agctatagta aaggttcacg gggtctttcc gtctagccgc gggtacaccg
    16021 catcttcacg gcgatttcaa tttcactgag tctcgggtgg agacagcctg gccatcatta
    16081 tgccattcgt gcaggtcgga acttacccga caaggaattt cgctacctta ggaccgttat
    16141 agttacggcc gccgtttact ggggcttcga tcaggagctt ctcttgcgat aacaccatca
    16201 attaaccttc cagcaccggg caggcatcac accctatacg tccactttcg tgtttgcaga
    16261 gtgctgtgtt tttaataaac agttgcagcc agctggtatc ttcgaccggt tcaaccttca
    16321 tccgcaaggg actacaatct acgccggcgc accttctccc gaagttacgg tgctattttg
    16381 cctagttcct tcacccgagt tctctcaagc gcctgagtat tctctacctg accacctgtg
    16441 tcggtttata gtacggttta taataacctg aagcttagtg gcttttcctg gaagcgtggt
    16501 atcgattact tcatctccgt agagactcgt catcacttct cggtgttaag gaagaccgga
    16561 tttgcctaat cttcccacct accggcttaa acagacatcc aacagtctgc taacctaacc
    16621 ttctccgtcc ccacatcgca gttattacaa gtacgggaat attaacccgt ttcccatcga
    16681 ctacgctttt cagcctcgcc ttaggggccg actcaccctg ccccgattaa cgttggacag
    16741 gaacccttgg tcttccggcg aacgagtttt tcactcgttt tatcgttact tatgtcagca
    16801 ttcgcacttg tgatatctcc agcagacttc tcaatccacc ttcatcgact tacacaacgc
    16861 tcccctaccc aacagacttt cgtctgatgc cgcagcttcg gtgctatatt tgagccccgt
    16921 tacatcttcc gcgcaggccg actcgactag tgagctatta cgctttcttt aaatggtggc
    16981 tgcttctaag ccaacatcct agctgtctaa gccttcccac ttcgtttccc acttaatata
    17041 gactttggga ccttagctgg cggtctgggt tgtttccctc tccacgatgg acgttagcac
    17101 ccaccgtgtg tctcctgagt atcactcttc ggtattcgca gtttgcatcg ggttggtaat
    17161 ccgggatgga cccctagccg aaacagtgct ctacccccga aggtgtccgc tcaaggctct
    17221 acctaaatag atttcgggga gaaccagcta tctcccggtt tgattggcct ttcaccccca
    17281 gccacaagtc atccgctaat ttttcaacat tagtcggttc ggtcctccag ttagtgttac
    17341 ccaaccttca acctgcccat ggctagatca ccgggtttcg ggtctatacc ttgcaactac
    17401 acgcccagtt aagactcggt ttccctacgg ctcccttatt cagttaacct tgctacaaaa
    17461 tataagtcgc tgacccatta tacaaaaggt acgcagtcac aagattactc ttgctcccac
    17521 tgcttgtacg cacaaggttt caggttctat ttcactcccc tcgccggggt tcttttcacc
    17581 tttccttcac agtactggtt cactatcggt caatcaggag tatttagcct tggaggatgg
    17641 tccccccatc ttcaaacagg atatcacgtg tcccgcccta cttgttgtta gcctagtacc
    17701 acaatggagt tttaaggtac gggattatca ccctctacga ttgtccttcc cagaacattc
    17761 ccctaacttt actgctatca ctaactggct ctttcgcgtt cgctcgccgc tactaacgaa
    17821 atctcggttg atttcttttc ctcggggtac ttagatgttt cagttctccc ggtttgcctc
    17881 acacagctat gtattcactg ggtgatagta ggttcttcac ctactgggtt tccccattcg
    17941 gacatcttgg attaaacgcc tcttatcgac tcatccaagc ttttcgcaga ttagcacgtc
    18001 cttcttcgcc tctgattgcc aaggcatcca ccttgtgcgc ttagtcactt aactatacaa
    18061 cctcaaatgt tttctaaaac ttctcaaaaa taaaagtaaa aaaacaatga agtcagtttt
    18121 acaactaaac acttgactgc ttttgttcag tcaagatttt ttctactcag actttcaatt
    18181 tattcgttat cacttatgta taattactta tgcgtaacga acttgaaata tctctcagtt
    18241 tttcagcttg tttccatatt gttaaagaac aaagataatg gctttcgcct atcatcatac
    18301 ctaaaattgc aaataatccg gtatttttaa ccgcttgtaa tcttaggtat gatgataaag
    18361 tcacgatgat tggtggagat aagcgggatc gaaccgctga cctcctgcgt gcaaggcagg
    18421 cgctctccca gctgagctat atccccattt catcttccgt tttcttcact catagtcgct
    18481 tcgctttcgc ttgccttctg agtggtgggt ctgagtggac ttgaaccacc gacctcaccc
    18541 ttatcagggg tgcgctctaa ccacctgagc tacagaccca acggatgacc ttcggttttt
    18601 tgtcttctt
//
LOCUS       DANLRK010000029        18141 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.029, whole genome shotgun sequence.
ACCESSION   DANLRK010000029 DANLRK010000000
VERSION     DANLRK010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 18141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18141
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.029"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(62..4048)
                     /gene="hrpA"
                     /locus_tag="PXA33_000748"
     CDS             complement(62..4048)
                     /gene="hrpA"
                     /locus_tag="PXA33_000748"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HDL5120103.1"
                     /translation="METPLQKNHKKPPLVKLENITTADFRRLLGRLKGAENIKNIDHK
                     RSVLAEISQDLTACQARFFARTQHAQNLKIDYPDLPVSARREEILKLISENQVVVIAG
                     ETGSGKTTQLPKMCLELGRGVKGLIGHTQPRRIAARSVATRIAEELKSELGETVGYKV
                     RFNDQVSENSLIKLMTDGILLAEIQHDSYLNQYDTLIIDEAHERSLNNDFILGYLKQI
                     LHKRPDLKVIITSATIDVERFSKHFNNAPIIEVSGRTFPVEVRYRPIVEDQDQLQGIL
                     NAVDELQAEGRGDILIFMNGEREIRDTAEALQKQELRHSEILPLYARLSAAEQQRIFQ
                     PSGGLNRIILATNVAETSLTIPNIKYVIDTGTARISRYSYRTKVQRLPIEPISQASAN
                     QRKGRCGRTSEGICIRLYSEEDFNSRPQFTDPEILRTNLAAVILQMTSLGLSDIANFP
                     FVDAPDSRQIQDGIRLLEELQAIIPNEKIPSTACGGGLGRGEIRSNDGTPPLPTSPRK
                     RGDGFRLTPIGRQLAQLPVDPRLGRMVIEAAKNGSLHEVMMIVSALSIQDPRERPQEK
                     QQSADDKHRRFADKESDFLAFVNLWHFIQAQQKELSKNQFRNLCRKDYLNYNRVREWQ
                     DIYHQLRLAVREMGLKINSEPASYQPIHTALLAGLLSHIGLKDNEKMHYLGARNAHFF
                     IFPNSALFKKQPKWVMAAELVETTKLWGRMVARIEPEWVEPLAVHLTKSSYSEPHWAK
                     SKGAVMASEKVSLYGIPIVAQRSVNYGSIDPTVSREIFIRSALVEGECHNNYKFFKEN
                     HRLIREVEALEHKSRRRDILVDEQVLFEFYDQRIGTEVVSSKHFDTWWKQASKKDPEL
                     LNFEKSFLINDNANLASELDFPNFWHQGDLKLKLSYQFEVGKDHDGVTVHIPLPLLNQ
                     VEPEGFDWQIPGLRHELIVSLIKSLPKSLRRNFVPAPNYADAFLGRAEPFKKPLLDNL
                     SHELRRMTGVLVEPEQWDLSQLPPHLRMTFRVIDDKGKKLQESENLDELKFALKDEVQ
                     NTLSNIADDGIEQSGVHIWNFAELPQFYEQKKAHFSVKAYPAIVDEQTAVGVKLFETE
                     FEQARAMQAGLRRLLLLNVPSPIKYLHEKLPNKAKLGLYFAPFGKVLELIDDCIACAV
                     DQLVEEFGGFVWTEEKFTQLHEFVRANLNDKTVEIAKQVEKCLTLAYELNKRLKGKLD
                     FTMAFALSDIKTQIEGLIYPDFVTKTGYARLADLYRYLTAIERRLDKLLVDSNTDRAK
                     MLRVEAVQEAYKQLLAKLPKSKAVPDEVLEIRYMIEELRVSLFAQQLGTKYPISDKRI
                     LNVINEIK"
     gene            complement(4152..4604)
                     /locus_tag="PXA33_000749"
     CDS             complement(4152..4604)
                     /locus_tag="PXA33_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HDL5120104.1"
                     /translation="MTLQFNAVALLLVVLIILGLISQNSAVTISAAVLLIMQQTLLSK
                     FIPFVDQYGLKIGIIILTIGVLSPLVSGRITLPELAQLLNWKMALSIIAGVLVAWLGG
                     RGVNLMGSQPVLVTGLLIGTVIGVAFLKGVPVGPLIAAGILSLIVGKT"
     gene            complement(4667..4936)
                     /locus_tag="PXA33_000750"
     CDS             complement(4667..4936)
                     /locus_tag="PXA33_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5120105.1"
                     /translation="MDAAMSLRFDKLRFVKRLQEANQTPEMAEAFANALDEALEQSQS
                     PLATKADLQLELEKLKNEINTTIFKAITLNITILGFLMAIMKFIN"
     gene            complement(5034..5630)
                     /gene="grpE"
                     /locus_tag="PXA33_000751"
     CDS             complement(5034..5630)
                     /gene="grpE"
                     /locus_tag="PXA33_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005712850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HDL5120106.1"
                     /translation="MTTQNEHLQEEVKQEEIQPEVVTEETQAEEQNTPQEEPLALAQA
                     RIAELEAYIAEADKREQDIQLRAQAEIQNIRRRAEQDVEKAHKFALEKFSKELLTVVD
                     NLERGLDALDKAVTDETTQALVDGVEMTHKEFINTLAKFGVVAIGEVGEAFNPELHEA
                     ISMQPAEGIEANHVSTVLQKGYTLHGRVIRPAMVMVAN"
     gene            5852..6946
                     /locus_tag="PXA33_000752"
     CDS             5852..6946
                     /locus_tag="PXA33_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDL5120107.1"
                     /translation="MKKLAGLFAAGLATVALTACNEEKAAEPKATAAEAKPQATNQTV
                     HLYTWTEYVPEGLLDEFTKETGIKVEVSSLESNETMYAKLKLQGKDGGYDVIAPSNYF
                     VSKMAKEGMLAELDHAQLPVIKELNQDWLNKPYDQGNKYSLPQLLGAPGIAFNTADYK
                     GETFTSWGDLWKPEFAGKVQLLDDAREVFNIALLKLGKNPNTTNPDEIKEAFEELKKL
                     RPNVLSFTSDNPANSFIAGEVSVGQLWNGSVRIAKKENAPVDMVFPKEGPVLWVDTLA
                     IPANAKNKENAHKLINYLLSAKVAEKLTLAIGYPTSNVEALKVLPKEITEDTAIYPSA
                     EVLQKSQWQDDVGDAIELYEKYYQELKAAK"
     gene            7082..8473
                     /locus_tag="PXA33_000753"
     CDS             7082..8473
                     /locus_tag="PXA33_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDL5120108.1"
                     /translation="MNLRWQEYPENARKLFKLSLPIFISQLSASGMGLADIVMAGLVS
                     DDDVSAIAVSNSIYFPLFLFVSGLLNAITPTVSYLNGSSNRHLIANQVRQGYWIVFAM
                     SIPLMAIFLNSHLILDLMQTPAEFSIKSQQYLAVMAVGLIPALLAVSLRCMNDGLSNP
                     KPAMRITFFGLLLNIPLNYIFIFGKFGLPEMGAVGCGVATAIVNLIMFFMLLHYCYTN
                     SAQKDIRLFEKWIDAPSGQILLKLCKLGLPIAFAIFTEVMLFSTSSLFLSPLGSQVVA
                     SHQAALQTSSLFFMIPMSFGIATTIMIGQTLGQKNVEGAKILSYHALITATALAIIAA
                     IIIILLKNFIPFAFTNNPISIAITAHLLIFAAVYQIPDAIQAVANGILRGYKHTQPIL
                     YVTLFCYWGVSMPLGYILARTDWIVQPMAAQGFWLVFCIGLSLAGGLLLWQMRKIQAV
                     PKAQLIAKLERIK"
     gene            8532..12056
                     /gene="recB"
                     /locus_tag="PXA33_000754"
     CDS             8532..12056
                     /gene="recB"
                     /locus_tag="PXA33_000754"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HDL5120109.1"
                     /translation="MKNLNPIQLPLNKSVLIEASAGTGKTFTIANLYLRLLLGIGCNP
                     LTVEQILVVTFTKAATEELKNRIRKNIQQCADFLKDQADGLEVESTKSYRNNLDFLAQ
                     IYPLIPNIHEALLRLSIAEREIDTASVFTIHGFCQKMLVQFAFESGVRFDLDLQPNQS
                     DLLKKLSEEVWREQFYPQDLAITYAVAEQLGTPEYALNAVRRYLSTELPEPNASLNQD
                     IAEHLAQYQQFINEVKSYWKTHSDELVETIRHDLKHKITLHGGWYRATTFEAGVEVMN
                     QWANSQHNHFPAGLYLFTTEKIAAGTKKSCEPIDSPHLAKNQAFLTAYKERFENSLSA
                     MLQYQFLIALKAKLAEYKQSHPQRSFDDLLTMLNQALKNLHTGEALSQKIRAMYPFAM
                     IDEFQDTDLTQYEIFSQIFMAENAENGFIMIGDPKQSIYKFRGADIFTYLRAAKQADE
                     QFTLGKNWRSLPEVVQAVNNLFSFANDNSPFLYEDIGFHPVEADSQKGQLVSEQGHFV
                     GYITENSKLDDLAEMCAYQIHQQLEAMAQGKFGVEKNGEFQSFAAKDIAILVRKGSQA
                     KRIKKALAKRNIKSVYLAESASVFGSEIATELCWLLQAALNPYHYKALLSALGSTLWG
                     LTAAEIHRYKENEQLWDLKVNQFVEYSNIWKEQGVLPMLHKIYLEQGIIERLKGLENA
                     DRLITDLLHLTEILQETSQALESEAELLRWYEKQVNSPDFNDEQQLRLESEEELIKIV
                     TIHGSKGLQYPIVWLPFVSQKSEGDTQTALSLYRNSQGALDWCLGSEEASFSLQYEAY
                     AEDLRLLYVALTRAESQLNLVLPKEISGWSPLHYLLADGEIPTGKVPVSDYFSQKRIA
                     ANLVEVAGELPFSLWEGEQSSDDYQAKTFSGNIRVVGQITSFTSLYAQHERFSEGKSE
                     RVMDLAQDRDVHIETIEAGYSSETAAFSPFQFPHGIKVGTVLHHFFEHCQFNEQIDRE
                     AVAKVCEQLGLSEEWIEPTALWFERILTTPLAEANFCLKAIDETKRLNEWQFYLRLKN
                     DKALHQLNALLKQHSPLAKTLPELQLPQLEGFVRGFVDCIVQVEEKFYLIDYKSNFLG
                     YLPQNYAKEHIQREMGRQRYDLQYLLYTLALHRYLTARLGEKYDYERDFGGVAYLFLR
                     AMNGENDNGVYFDKPTKQLIEEMDRLFG"
     gene            complement(12098..12580)
                     /gene="greB"
                     /locus_tag="PXA33_000755"
     CDS             complement(12098..12580)
                     /gene="greB"
                     /locus_tag="PXA33_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HDL5120110.1"
                     /translation="MAKSNYITRSGWQALDQELKFLWREERPKVTQAVSDAAALGDRS
                     ENAEYIYGKRRLREIDRRVRFLSKRLEVLQIVDYHPKQEGKVFFGAWVELENEEGEAK
                     QYRIVGCDEFDPAKNWISIDSPVARALIGKQVDDEIRVETPLGKVLLYINKIWYEQAS
                     "
     gene            complement(12631..13911)
                     /gene="pepB"
                     /locus_tag="PXA33_000756"
     CDS             complement(12631..13911)
                     /gene="pepB"
                     /locus_tag="PXA33_000756"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HDL5120111.1"
                     /translation="MTAMKISLSTQNAPEIWGKNAILSANDAGFTVHNPNNDLATIQK
                     AARKIKNQGISTVELTGDSWDLESCWAFHQGFHSVKNQGSVQYPDLDEQQAEFDNRVK
                     CATFVRELINKPSDKLTPEKLAEQAVEFIQKMAKPNTVSYQIIRGEALQEQGYHGIWT
                     VGKGSANPPAFLVLDFNPTNDENAQVLACLVGKGITFDTGGYSLKPSDSMSTMRTDMG
                     GAALLTGSLAFAIARGLNKRVKLYLCCAENMVSHNAFKLGDIITYRNGVTVEVLNTDA
                     EGRLVLADGLIDACKQNPQFIIDAATLTGAAKVAVGNDYHSVLSMDDNLTEALFKSAK
                     TENEPFWRLPFEEFHRGQIASSFADIANIGSVPVGAGASTATAFLSYFVENYQQNWLH
                     IDASATFRKTASDLWAAGATGLGIRTVANLLLSK"
     gene            complement(14050..14871)
                     /locus_tag="PXA33_000757"
     CDS             complement(14050..14871)
                     /locus_tag="PXA33_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010869033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase, lipoprotein e(P4) family"
                     /protein_id="HDL5120112.1"
                     /translation="MKNIKLSAMSLTALFVLTACSHTHKNSDEHHQMVLQEQATLGIN
                     WMQQSGEYQALAHQAFNAAKVAFDQAKVKKGKKKAVVVDLDETMVDNSLYAGWQVKNH
                     KPFDGESWTRWVNARQTGAIAGAVEFNNYVNSHKGTMFYVSNRKDSSEKAGTIDDMNK
                     LGFSGVSEQTLLLKKDKSNKTPRFEEIEKQGYEIVLYIGDNLNDFGDATYRKSNAERR
                     AFVAENSQQFGKKFIMLPNPNYGDWEGGLDKDYYKGNAESRVKIRHNAIKAWDGK"
     gene            15019..15962
                     /locus_tag="PXA33_000758"
                     /pseudo
     CDS             15019..15962
                     /locus_tag="PXA33_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251085.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
     gene            complement(16075..17238)
                     /locus_tag="PXA33_000759"
     CDS             complement(16075..17238)
                     /locus_tag="PXA33_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HDL5120113.1"
                     /translation="MKRKTLATLVLIAALGIGGYFYYQQQTNKPQIHYITQAVTRAEI
                     NKNVLATGSVRANKRTEVGAQVSGKIQKLYVELGQSVKQGDLIADIDSSNQSNTLSTA
                     QARLSSYKAQLTSAQVALEVAQSNYNRLNKLYRANSASLNDLETAKNTLASAKATVDN
                     IKAQLKSAEISVNDAKTNLTYTQIISPMDGVIVSVPVSVGQTVNSNQTSPTIVQVADL
                     SKMLIKLEISEGDIAQVKVGQKVKFSTLAEPNREYSATIDSVDPALTTLTDNNYTEQS
                     GNTEAVYYYANVSVDNADNSLRIGMTTQAQITIDKREQVLVVPTSVIKKRGKENYVLV
                     LENEQSVERKIQIGISDTQNTEVLAGLNEGDQVIVTQRADDEKISTARGPRMF"
     gene            complement(17304..18119)
                     /locus_tag="PXA33_000760"
     CDS             complement(17304..18119)
                     /locus_tag="PXA33_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL5120114.1"
                     /translation="MTLPFRAIISDLDGTLLNANHKIGDFTIDTLEQLSKKGVDIFFA
                     TGRNHPDVSQIIGKVNVKNAMLVTSNGARANNLEGKKILNHYLPEDLAFELMNIEFDD
                     QNVCLNSYQGDEWFINKEIAELKKFHQDSGFSYQVVNFAHHHGRNTEKVFFIGKTAEA
                     LVPIEHYIRDTYGDRVQMTYSALLCLEVMAKGVCKANALAELVKLRGYTLADCIAFGD
                     GMNDVEMLSLAGKGCLMGNADPRLKAVLPNNEVIGFNKDEAVASYLRATFGIV"
BASE COUNT         4907 a         3800 c         3938 g         5496 t
ORIGIN      
        1 aaacaagcgg tcagagttgc aaaatttttt acttatctga ccgcttgttg tatagggaat
       61 attactttat ttcattgatc acattcaaaa tccgcttatc cgaaatcggg tattttgtgc
      121 cgagctgttg agcgaacaga ctgacccgca gttcttcgat catatatcga atttccagta
      181 cctcgtccgg caccgctttg gattttggca gtttggcaag gagctgttta tatgcctctt
      241 gcactgcttc cacccgtagc attttggctc ggtcggtgtt gctgtccacc aacagtttat
      301 ccaaacggcg ttcgatagcg gtcaaataac ggtataaatc tgcaaggcgg gcgtagccgg
      361 ttttggtgac aaagtcgggg taaatcagcc cttcgatttg ggttttgatg tcgcttaacg
      421 caaacgccat cgtaaaatcg agcttgcctt ttaaccgctt gtttagctcg taggccagtg
      481 tgaggcactt ttcaacctgt ttggcaattt ctacggtttt atcgtttaaa tttgcccgca
      541 caaattcgtg cagttgggtg aatttttcct ccgtccagac aaacccgccg aactcttcta
      601 ccagctgatc taccgcacag gcaatgcagt cgtcaatcag ctccaatact ttgccgaacg
      661 gggcaaaata taagcccagc ttggctttgt tcggcagttt ttcgtgtaga tatttaatcg
      721 gcgatggcac atttagcaac agcagacggc gaagtcctgc ctgcattgca cgagcctgct
      781 caaattccgt ttcaaacagt ttcaccccca cagccgtttg ttcatcgaca atcgcagggt
      841 aggctttcac gctgaaatgg gcttttttct gctcatagaa ctgtggcaat tcggcaaaat
      901 tccaaatatg cacaccactt tgctcaatgc cgtcatcggc aatgttggaa agggtgttct
      961 gcacctcatc tttcaaggca aatttcagct catcaaggtt ttcgctctct tgcagtttct
     1021 tgcctttatc atcaatcaca cggaaagtca tccgcaaatg tggcggcagt tggcttaaat
     1081 cccactgttc aggctcgacc aatacacccg tcattcggcg tagttcgtgg cttagattgt
     1141 ccagcaacgg ttttttaaac ggctcggctc gccctaaaaa ggcgtccgca taattcggcg
     1201 ccggcacaaa atttcgccgt agcgatttcg gtaaagattt aatcagcgag acgatcagct
     1261 cgtggcgtag ccccggaatt tgccaatcga aaccttcggg ttccacttgg ttaagcagcg
     1321 gtagcggaat atgcaccgtc acgccatcgt gatcttttcc gacctcaaac tgatagctca
     1381 gtttgagttt taaatcgcct tgatgccaga aattcgggaa atccagctcg ctggcaaggt
     1441 tggcgttgtc gttgatcagg aacgattttt caaagttcag cagttcagga tcttttttgc
     1501 ttgcctgttt ccaccaagta tcaaaatgct tgctcgacac aacttcggtg ccaatccgct
     1561 gatcgtaaaa ctcaaacagc acctgctcgt ccaccaaaat atcacggcgg cgggatttgt
     1621 gttccaatgc ctcaacttca cggatcaagc ggtgattttc tttgaaaaat ttgtaattgt
     1681 tgtgacattc gccttccacc aacgccgagc ggataaaaat ctcacggctg acggttgggt
     1741 ctatcgagcc ataattcacc gagcgttgtg ccacaatcgg aatgccgtag agcgacactt
     1801 tttccgatgc cattaccgct ccttttgact tcgcccagtg tggttcgctg taactggatt
     1861 tagtgaggtg tactgccaac ggttccaccc attccggctc aatgcgagcg accattcgtc
     1921 cccaaagttt ggtagtttcc accagctctg cggccattac ccatttcggc tgttttttga
     1981 aaagtgccga gttcgggaaa atgaaaaagt gggcatttct tgctcctaaa tagtgcattt
     2041 tttcattatc tttcaagccg atatgcgaca acaagccggc gagcagggcg gtgtgaatcg
     2101 gctgataact tgccggctca ctgttaattt ttaagcccat ttctctaact gcaaggcgga
     2161 gttggtggta gatatcctgc cactcccgca cacggttgta gttcaaataa tccttacggc
     2221 agagattacg gaactggttt ttcgacagtt ctttttgttg ggcttggata aagtgccaca
     2281 aattcacaaa ggctaagaaa tcgctctctt tatcggcaaa acggcggtgt ttatcgtctg
     2341 ccgactgctg tttttcctgc ggacgctcac gagggtcttg gattgagaga gctgacacaa
     2401 tcatcatcac ttcgtgcaac gaaccgtttt tcgccgcctc gatcaccatt ctgcctaagc
     2461 gaggatcgac tggcagttgg gcgagttggc gaccgattgg ggttaatcta aacccatccc
     2521 cccgtttacg gggggaggtt gggagggggg gagtgccatc attagaacgg atttcccccc
     2581 tccctagccc tcccccgcaa gcggtggagg ggattttttc atttggaata atcgcctgta
     2641 attcttccaa caaccgaatc ccgtcctgaa tttgtcggct gtcaggggca tcaacaaacg
     2701 ggaaattggc aatatcactc aatccaagcg aagtcatttg taaaataacc gctgcaaggt
     2761 tagttcgtag aatttcagga tcggtaaatt gtggacggct gttgaaatcc tcttctgaat
     2821 agaggcggat acagatccct tccgaggttc gcccacaacg ccctttccgc tggttggcgg
     2881 aggcttgcga aatcggctca atcggcaagc gttgcacttt ggtgcggtag ctataacgag
     2941 aaatacgagc cgtgccggtg tcgatcacat atttaatatt cggaatagtg agggaggttt
     3001 ctgccacgtt ggtcgccaga ataattcggt ttagcccacc gcttggctgg aaaatccgtt
     3061 gttgttcggc agccgataag cgggcgtaaa ggggtaaaat ttcgctatgt cgcagttcct
     3121 gcttttgcaa ggcttcggca gtgtcacgaa tttcccgttc accgttcata aaaatcagaa
     3181 tatcgccacg cccttcggct tgcagttcgt ccaccgcatt tagaatacct tgcagttgat
     3241 cttgatcttc cactatcggg cgataacgca cttccaccgg gaatgtccga ccagagactt
     3301 caataatcgg ggcgttgttg aaatgtttgg aaaaacgttc tacatcaata gttgccgagg
     3361 tgatgatcac tttcagatcg gggcgtttgt gcagaatttg tttcagataa cccaaaataa
     3421 aatcgttgtt cagcgaacgc tcgtgagctt cgtcaataat cagcgtatcg tactgattta
     3481 aatagctgtc gtgctggatt tccgccagca gaataccgtc cgtcattagc ttgattaagc
     3541 tgttttcact gacttgatcg ttaaagcgga ctttgtaacc gaccgtctcg cccagttccg
     3601 atttcagctc ttcggcaatg cgagtcgcca ccgaacgtgc ggcaattcgg cggggctggg
     3661 tatgcccaat caagcccttc accccacgcc cgagttctaa gcacattttc ggtaactggg
     3721 tggttttgcc cgaaccggtt tcccctgcga tcaccaccac ctgattttcg ctaatcagtt
     3781 ttaaaatttc ctcacggcga gcggaaacag gcagatcagg gtagtcaatt ttcaggtttt
     3841 gggcgtgctg agttcgtgcg aaaaaacgtg cctgacaagc ggtgagatct tgcgaaattt
     3901 ctgccaaaac tgaccgcttg tgatcgatgt ttttaatatt ttccgcccct tttaagcgac
     3961 cgagcaggcg gcggaaatcg gcggtggtga tattttccag cttcacaagc ggtggtttct
     4021 tatgattttt ttgcaaaggt gtttccattt aatttctctt ttgcattaag accttgtgat
     4081 ttattgtagg gtgggcgtaa gcccacgtaa cagaataggg attttatgcg tggacttacg
     4141 tccaccctac attaggtttt accaacaatc agtgacaaaa tccctgccgc aatcaacggc
     4201 ccgaccggta cgccttttaa gaacgccacg ccgatcaccg tgccgattaa aagcccagtt
     4261 accaacaccg gctggctgcc cattaaattc acgcccctgc cacctagcca cgctaccagc
     4321 acgccagcaa ttattgagag tgccattttc cagttcaata gttgggcaag ttcgggaagc
     4381 gtaattcgcc ctgaaaccaa ggggctaagt acgccgatgg ttaaaatgat aatcccgatt
     4441 tttaagccgt attgatcgac aaaggggata aatttagaaa gtaaggtttg ttgcataatc
     4501 agcaatacgg cggctgaaat agtaaccgca ctgttttggc tgatcagccc taaaataatc
     4561 agcaccacaa gtagaagtgc cactgcgtta aattgtaaag tcataggatt aaaacgtaaa
     4621 aacccttact tcatagaagt aagggagtgg ttaattaaac gttgagctag ttaataaatt
     4681 tcattattgc cattaaaaag ccaagaattg tgatatttaa ggtaattgct ttaaatatcg
     4741 tggtattgat ttcatttttt agcttttcaa gttcaagttg caaatcagct tttgttgcta
     4801 gtgggctttg actttgctct aaggcttcat cgagagcatt agcaaatgcc tcagccattt
     4861 caggtgtttg attggcttct tgcaagcgtt ttacaaaacg taatttgtca aatcgtaggc
     4921 tcatagctgc atccatttca attatcctct tgtggttaag ttataaacta atttagcata
     4981 atcacaagca ggaattttag gagattttgt gatcttcgtc gaaaatcgac aatttagttt
     5041 gccaccatca ccattgccgg gcgaatcact cgtccgtgaa gcgtgtagcc tttttgtagc
     5101 actgtgctga catggttggc ttcaattcct tcggcaggtt gcattgaaat agcttcgtgc
     5161 aattcagggt taaatgcctc gcctacttcg ccaattgcca ccacaccaaa tttagccagt
     5221 gtattgatga attctttatg agtcatttca acgccgtcaa ctaacgcttg agtggtttca
     5281 tcggtcacgg ctttatcaag ggcatctaat ccacgttcaa ggttatcaac cactgttaac
     5341 aattctttgg agaatttttc taaagcgaat ttgtgagctt tttcaacatc ttgctctgca
     5401 cggcggcgga tattttgaat ttctgcttga gccctaagct gaatatcttg ctcacgtttg
     5461 tcggcttctg caatataggc ttctaactcg gcaattcgag cttgagcgag agctaacggt
     5521 tcttcttgag gtgtattttg ttcttccgct tgagtttctt cagttacaac ttcaggttga
     5581 atttcttctt gttttacttc ttcttgtaag tgttcatttt gagtggtcat tttttatcct
     5641 ttaaaagact aatctggaaa tgtaaatctt ccttaatata gcgataaatt aagggatttc
     5701 aagcatatct ctttcttttt gcgaaaataa ttcacttttt aatcagaaaa ggcttgcaaa
     5761 atacgggggg gggcttaaaa tgccctagtc ttttccgccc ttaggcaagg ctatattttt
     5821 taatcacttt tctttaacgg agaaaactta aatgaaaaaa ttagcgggtt tatttgcagc
     5881 aggtttagca acagttgcat taacagcgtg taatgaagaa aaagcggcag agccaaaagc
     5941 aactgcggct gaagcaaaac ctcaagcaac aaatcaaaca gttcaccttt atacttggac
     6001 tgaatatgtg cctgaaggtt tgttagatga atttacgaaa gaaaccggca ttaaagttga
     6061 agtttcaagt cttgaatcta acgaaacaat gtacgctaaa ttaaaattac aaggcaaaga
     6121 tggcggttat gatgtaattg ctccatcaaa ctactttgta tctaaaatgg cgaaagaagg
     6181 aatgttagcg gaattagatc acgcacaact tccggttatt aaagaattaa accaagattg
     6241 gttaaataaa ccttacgatc aaggaaacaa atattcatta cctcaattat taggtgcgcc
     6301 gggtattgca tttaatacgg cagattacaa aggtgaaaca ttcacttctt ggggtgattt
     6361 gtggaaacct gaatttgcag gtaaagtaca attattagat gatgctcgtg aagtattcaa
     6421 cattgcgtta ttaaaattag gtaaaaaccc aaatacaact aatccggatg aaatcaaaga
     6481 agcctttgaa gagttgaaaa aattacgtcc aaatgtgctt tcatttacct ctgataaccc
     6541 ggcaaactca tttattgctg gtgaggtttc tgtaggtcag ttatggaacg gttctgtacg
     6601 tattgcgaag aaagaaaacg caccggttga tatggtattc ccgaaagaag gtcctgtgct
     6661 ttgggtcgat actttagcca ttccggcaaa tgcgaaaaac aaagaaaatg cacataaatt
     6721 aatcaactac ttattaagtg caaaagtagc tgaaaaatta acgttagcga ttggttaccc
     6781 aacttcaaac gtagaagcat taaaagtatt accgaaagaa attactgaag atacagcaat
     6841 ttacccatct gcagaggttt tacaaaaatc tcaatggcaa gatgatgttg gtgatgcaat
     6901 tgagctttac gaaaaatact accaagagtt aaaagcagca aaataatagc tgattaattc
     6961 tgaagcctct agggagcaat ctttagaggc tttctctttt gagttgcaaa aaatttggca
     7021 aaaacgaccg ctttctaagg tacaatcata aaaacatact tagattttga gagattaaac
     7081 catgaactta agatggcaag aatatcctga aaatgcccgc aaattattta aactatccct
     7141 acccattttt atttctcagc tttcagcttc cggaatgggg ctggcggata ttgtaatggc
     7201 tggcttggtg agtgatgatg atgtctcggc gattgcggta agtaattcga tttactttcc
     7261 gctctttctg tttgtgtcag ggttgctgaa tgcaattacc ccaaccgttt cttacttaaa
     7321 cggctcgagc aatcgccatt taattgctaa ccaagtaagg cagggctatt ggattgtgtt
     7381 tgcaatgtct attccattaa tggcaatttt cctaaacagc catctgattt tagacttaat
     7441 gcaaacaccg gctgagtttt caatcaaatc gcaacaatat ttagcagtaa tggcagttgg
     7501 gcttattcct gctttgctgg cggtgagttt acgttgtatg aacgatggat tatctaaccc
     7561 taaacctgcg atgcggatta cctttttcgg tttattgctg aacattccgc taaactatat
     7621 tttcattttc ggcaaatttg gcttgcctga aatgggagcg gtaggctgtg gtgtagcaac
     7681 cgcaattgtg aacttgatta tgttttttat gttgttgcat tattgctaca ccaattctgc
     7741 ccaaaaagat attcgtttgt ttgagaaatg gattgatgct ccaagcggtc aaattttgct
     7801 aaaactttgc aaattagggc tgccgattgc ctttgcgatt tttaccgaag tgatgctgtt
     7861 ttcgacctca tcgctgtttc tgtcaccact tggttcacag gtggtggcta gccaccaagc
     7921 tgcattgcaa accagctcgc tattttttat gattccgatg tcgtttggca ttgcaaccac
     7981 cattatgatc ggacaaacac ttgggcagaa aaatgtggag ggggcgaaaa tcttaagtta
     8041 ccacgcactg attaccgcaa ccgctttagc tattattgcc gccatcatta ttattttgtt
     8101 gaaaaatttt atcccttttg cgtttaccaa caacccgatt tcgattgcga ttaccgctca
     8161 tttattgatt tttgcggcgg tttatcaaat accggatgcc attcaagcgg tagcaaacgg
     8221 cattctgcgt ggttataaac atacccaacc gattttatac gttactctgt tttgttattg
     8281 gggggtgagt atgccgctgg gctacatttt ggcaagaacc gattggattg tacaaccaat
     8341 ggctgctcaa ggtttttggt tagtcttttg tatcggctta agtttggctg gtggcttgtt
     8401 attatggcaa atgcgaaaaa tacaagcggt gccaaaagca caattaattg caaaattaga
     8461 acggattaaa taacagattc tctgctatgg aacatagcag tgctttttac ttttagaaga
     8521 taactttgct gatgaaaaac ctaaatccta tccaactacc tttgaataaa tccgttttaa
     8581 tcgaagcgtc ggcaggtaca gggaaaacct ttaccattgc caacctgtat ttgcgtttat
     8641 tgctcggcat tggttgcaac ccgttaacgg tggaacaaat tttggtggtg acatttacca
     8701 aagcagcaac cgaagagttg aaaaaccgta tccgtaaaaa tattcagcaa tgtgccgact
     8761 ttttaaaaga tcaagctgat ggtttggaag tggaaagtac aaaatcctat cgaaacaacc
     8821 ttgatttttt ggcacagatt tatccgctca ttccgaatat tcacgaagca ttgttacgtt
     8881 taagtattgc cgaacgggaa attgataccg ccagcgtgtt taccattcac ggcttttgtc
     8941 aaaaaatgct ggttcagttt gcgtttgagt cgggcgtgcg gtttgacttg gatttacagc
     9001 caaaccaaag tgatctactt aaaaaattaa gtgaagaagt ctggcgagag caattctatc
     9061 cgcaagattt agccatcact tatgcggttg ccgaacaact tggtacgccg gaatatgccc
     9121 tcaatgcggt aaggcgttat ttatctactg aattacctga accaaatgcc tctttaaatc
     9181 aagatattgc cgagcatttg gctcaatatc agcagtttat taatgaggtg aaatcctatt
     9241 ggaaaacgca ttcagatgaa ctggttgaaa ctatccgcca cgatttaaag cataaaatta
     9301 cgctgcacgg aggttggtat cgagcgacca cttttgaagc cggtgtggaa gtgatgaacc
     9361 agtgggctaa ttcgcaacat aaccatttcc cggcagggct ttatttattc actacagaga
     9421 aaattgcggc tggcacgaaa aagagctgtg agccgataga ttcccctcat cttgcaaaaa
     9481 atcaggcttt tttgaccgct tacaaggaac gttttgaaaa cagcttgtca gcaatgttgc
     9541 agtatcaatt tttaattgct ctcaaagcaa aattggcaga gtacaaacaa tcgcacccac
     9601 aacgcagttt tgatgattta ctcacaatgc taaaccaagc gttgaaaaat ctacacacgg
     9661 gcgaagcctt gtctcagaaa attcgggcga tgtatccttt tgcgatgatc gatgagttcc
     9721 aagataccga tttaacccaa tatgaaattt tcagccaaat ttttatggca gagaatgcag
     9781 agaacggctt tattatgatt ggcgacccga aacagtcgat ctataaattc cgtggggcgg
     9841 atattttcac gtatttgcga gcagcaaagc aggctgatga acaattcaca ttgggcaaaa
     9901 actggcgttc gttgcctgaa gtggtgcaag cggtcaataa tttgttttct tttgcaaatg
     9961 ataactctcc gtttctttat gaggatattg gctttcaccc agtggaggct gatagccaaa
    10021 aaggtcagtt agttagtgag caagggcatt ttgtaggcta catcacggaa aacagtaaat
    10081 tagatgattt ggctgaaatg tgtgcttatc aaattcatca acagcttgag gcgatggctc
    10141 aaggcaaatt cggagttgaa aaaaacggcg aatttcaatc ctttgctgca aaagatattg
    10201 cgattttagt ccgaaaaggt tcacaagcta agcgaatcaa aaaggcgttg gcaaaacgta
    10261 atatcaaatc cgtctattta gctgaatcag cttctgtatt tggttcggaa attgcaacag
    10321 aattgtgttg gttattgcaa gccgcactga atccgtatca ttacaaggca ttattatcgg
    10381 cgttaggctc aaccttgtgg ggcttaacgg cggcagagat ccaccgctat aaggaaaatg
    10441 agcaactttg ggatctgaaa gtcaatcagt ttgtggaata cagcaacatt tggaaagagc
    10501 agggcgttct gccgatgttg cataaaatct acttggagca gggcattatc gaacgtttga
    10561 aaggtttgga aaatgcggat cgcttgatta cggatttatt gcatttaacc gaaattttgc
    10621 aggaaacctc gcaagcgtta gagtctgaag ctgaactgtt gcggtggtat gaaaagcaag
    10681 ttaattcgcc cgattttaat gacgagcaac aacttcggtt ggaaagtgaa gaggagctaa
    10741 tcaaaattgt gacgattcac ggctcgaaag gcttgcaata tccaattgtt tggttacctt
    10801 ttgtttcaca aaaaagtgag ggagacacgc agacagcatt aagtttatat cgcaattctc
    10861 aaggtgcgct ggattggtgt ttaggctcag aagaggcaag tttttcgcta cagtatgagg
    10921 cttatgcgga agatttacgt ttgctgtatg tggcattaac ccgcgcggaa tctcagctta
    10981 acttagtgtt gccgaaagag atttccggct ggtctccact ccattacttg ttagcagacg
    11041 gggaaatacc tacaggaaaa gtgcctgttt cagattattt tagccaaaaa cggattgctg
    11101 ctaatttggt tgaagtagcc ggggaattac ctttctcact gtgggaagga gaacaatctt
    11161 cagacgatta ccaagcaaaa acctttagtg gcaatatccg tgtggttggt caaattacca
    11221 gctttacttc actttatgcc caacacgagc gttttagtga agggaaatca gaaagggtaa
    11281 tggatttagc ccaagatcga gatgtacata tagaaaccat tgaggcaggt tattcaagtg
    11341 aaactgccgc tttctcgcca ttccaattcc cacacggtat taaagtgggc acggtgttac
    11401 atcacttttt tgagcattgc caatttaacg aacaaattga tagagaggct gttgctaaag
    11461 tatgcgagca attagggcta agcgaggaat ggattgaacc aacagcatta tggtttgagc
    11521 gtattctaac tacccctttg gcagaggcaa atttctgctt aaaagcgatt gatgaaacca
    11581 aacgattaaa cgagtggcaa ttctatttac gcttaaaaaa tgataaagcc ttacatcaat
    11641 taaatgcgtt attaaagcaa catagccctt tagcaaaaac tttacctgaa ttgcaattac
    11701 cgcaattaga aggttttgtg cgaggctttg tagattgtat cgtgcaagtg gaggagaaat
    11761 tctacttaat tgattacaaa tcgaatttct taggctatct gccacaaaat tatgcgaaag
    11821 aacacattca acgggaaatg ggcagacaac gctatgattt gcaatatctg ctttacactc
    11881 tcgccttaca ccgttatcta actgcccgtt taggtgaaaa atacgattat gaacgagact
    11941 tcggcggagt ggcttacttg ttcttaaggg caatgaacgg cgaaaatgat aacggcgttt
    12001 attttgacaa gccgactaag cagttgattg aagaaatgga tcggttgttt gggtaaatat
    12061 gtgtaggggc gaaaattttt cgccccttta tgggtaatta actcgcttgc tcataccaaa
    12121 tcttattgat ataaagcaac actttaccaa gcggagtttc taccctaatt tcatcatcaa
    12181 cctgtttgcc gattaaggct cgggcgacag gggagtcaat cgaaatccag ttttttgctg
    12241 ggtcgaactc atcgcaacca acaatacggt actgttttgc ctcgccctct tcattttcta
    12301 attccaccca agcgccgaaa aagactttgc cttcttgttt agggtgataa tcaacaattt
    12361 gtagcacctc tagccgctta gataaaaaac gcacacggcg gtcgatttct ctcaaacggc
    12421 gtttgccgta aatatattcg gcattttcgc ttcgatcacc caatgcggca gcatcagaca
    12481 ccgcttgagt cactttcggg cgttcttccc gccatagaaa ttttaattcc tgatctaagg
    12541 cttgccagcc tgaacgggta atgtaatttg atttagccat agttatttaa ttgtttatgg
    12601 agcaaatagt gatttgcccc tacgaagttt ctattttgaa agcaataaat tcgctactgt
    12661 tcttattccc agtccggttg ctcctgccgc ccataaatcg ctcgccgttt tgcggaatgt
    12721 ggcagaggcg tcaatatgta gccagttttg ctggtagttt tctacaaagt aagataaaaa
    12781 tgcggtggcg gtacttgccc cagcccccac cggcacagag ccgatattgg cgatgtctgc
    12841 aaaggaagaa gcaatttgcc cacggtgaaa ttcttcaaac ggtaaacgcc agaacggttc
    12901 attttccgtt ttggcagact taaatagtgc ttcggttagg ttgtcgtcca ttgaaagcac
    12961 cgagtggtag tcattgccga ctgcgacttt tgctgctcct gtgagggttg cagcatcaat
    13021 aataaattgt ggattttgct tgcacgcatc aattaaacca tcggcaagca ctaaacgccc
    13081 ttcagcatcg gtgtttaaaa cttcaacagt tacgccattg cggtaagtga taatatcgcc
    13141 taatttgaac gcattgtggc tgaccatatt ttccgcacag cagaggtaga gtttcactcg
    13201 tttattcaag cctcgagcaa tggcaaaagc aagtgagccg gttaaaagtg ccgctccgcc
    13261 catatcggtt cgcattgtgc tcatactatc gcttggtttt agactataac cgccggtgtc
    13321 aaaggtaatg cctttgccga ccaagcaagc cagtacctgt gcgttttcat cgttggttgg
    13381 gttgaagtct aatactaaga atgcaggcgg attggcagag ccttttccaa ctgtccaaat
    13441 gccgtgatag ccttgttctt gtaatgcttc gccacgaatg atctggtaag aaaccgtatt
    13501 cggttttgcc attttttgga taaactcgac cgcttgttcg gctaattttt caggggttaa
    13561 tttatctgat ggcttattaa ttaattcacg cacgaaagtg gcacatttta cacggttatc
    13621 aaattcggct tgctgttcgt ctaagtctgg gtattgtacc gagccttgat tttttaccga
    13681 atgaaatcct tgatgaaacg cccagcagga ttctaaatcc caactatcgc cggtcagctc
    13741 cacggttgaa ataccttgat ttttaatttt gcgggcggct ttttgaatag tggctaaatc
    13801 gttgttcgga ttatgtacgg taaaccccgc atcatttgcg gaaagaatag catttttacc
    13861 ccaaatttca ggggcgtttt gagtagaaag tgaaattttc attgctgtca ttatcggttt
    13921 ccttagaaaa tggattttgc tttatcctaa caaacttttg caaagtttta tacaaaaatg
    13981 accgcttgca gagcatagcc ttgacaagcg gtcattgttt cgtgttttta caacctgatt
    14041 aaggacggat tatttgccgt cccaagcttt aattgcattg tggcggattt taacacggct
    14101 ttcagcattg cctttgtagt aatctttatc taaaccgcct tcccagtcgc catagttcgg
    14161 gttcggtaac ataatgaatt ttttaccgaa ttgttggctg ttttcggcta cgaatgcacg
    14221 gcgttccgcg tttgatttac ggtaagtcgc atcgccgaaa tcgtttaagt tgtcgccaat
    14281 atagagcacg atttcatagc cttgtttttc gatttcttca aagcgagggg ttttgttcga
    14341 tttgtctttt ttcaacaaga gtgtttgttc gctcacacca ctaaagccta atttgttcat
    14401 atcatcaata gtaccggctt tttcactgct gtctttacgg tttgaaacat agaacatagt
    14461 tcctttgtgg ctattaacgt agttgttaaa ttccactgca ccggcaatcg ctccggtttg
    14521 gcgggcattt acccaacgag tccagctttc accatcaaac ggcttgtggt ttttaacctg
    14581 ccagccggca taaaggctgt tgtccaccat agtttcatct aaatctacta ccacggcttt
    14641 tttcttgcct tttttcactt tggcttggtc gaatgccact ttcgctgcat tgaatgcttg
    14701 atgggcaaga gcttggtatt cgccggattg ttgcatccag tttatcccta atgtagcttg
    14761 ctcttgcaac accatttgat gatgctcatc gctgttttta tgcgtgtgcg aacaagcagt
    14821 taatacaaat aaagctgtga ggctcatagc agaaagtttg atgtttttca tacgtatatc
    14881 cttagtttta ttgttgataa acagagcgat tatacctaaa aagagttgct tatggctaat
    14941 agtttattgc tcctattcac ttttaaagca aactaaggta gcctagtaac ctttttataa
    15001 caattacaca gaggctatat gcaaacatta ttaaaattta cacaatttat cagcaaaacc
    15061 ttcgccattt gggtggtgtt ttttgctttt atcgcagcac aatttccgga aacctttaag
    15121 caattcctgc cttggattcc ttatctgtta gggattgtga tgttgggaat ggggttaacc
    15181 ttaactttca aagattttgc cgaggtaacg aaaaatccga aatcagtgat tatcggcgtg
    15241 atcgctcaat tcgtggtatg ccgagtgtag cttatggatt ggcaaaagca tttaacttac
    15301 ccgcagattt agccattggt gtgattttag ttggttgttg cccgggcggt acttcttcaa
    15361 acgtgatgac ctatttggct cgtggcaaca cagcgttatc ggtagcttgt accactattt
    15421 caactttact tgccccagtg ctcacgccgg caattttcta tttatttgcc agccaatggt
    15481 tagaaattaa tgcaacggca atgtttattt cggtgttgca aatggtgcta ttgccaattt
    15541 ttgtgggcgt ggtgattcgt actattttca aacaaaaagt agagcaattt agccaaacaa
    15601 tgccattaat ttcagtgatt gcaatcgtat taattgtcac ggcggtggtg tcagttagcc
    15661 gagatcgtat tattgaatcc ggtttattaa tttttgcggt agtagcatta cacaacggtt
    15721 taggttattt aatcggttat ttggcgagcc gtttattaaa attaccgatt gcagacagca
    15781 aagcggtggc gattgaagtg gggatgcaaa actcaggatt gggggcagca cttgccgcat
    15841 tacactttaa agccaaccct atcattgcgg tgccaagtgc ggtatttagc ttctggcaca
    15901 atatttcagg cccgattttg gcgatgattt tcgctaaaat gaaaaacgat aacgaaaaat
    15961 aaaattcgtt ttaatacaga cgttttaatg ttttaataaa gaatagacaa acggttaaat
    16021 ttttctaatt ttttgcaaaa tgttcagaaa atttaaccgc ttgttgttta gaatttagaa
    16081 cattcttggt ccacgtgcag tgctgatttt ttcatcatcc gctctttggg taacaatcac
    16141 ttgatcaccc tcatttaagc ctgctaatac ttcggtattt tgcgtatccg aaatgccaat
    16201 ttggattttt ctttcaaccg actgctcatt ttccagcacc aacacataat tctctttacc
    16261 acgttttttg attacgctgg tgggtacaac taaaacctgt tcccgtttat caatagtaat
    16321 ttgggcttga gtagtcattc cgatgcgtaa actgttatcg gcattatcaa cggaaacatt
    16381 agcgtagtaa taaaccgctt cggtattacc cgattgttcg gtgtagttat tatcggtcag
    16441 cgtggtgagt gccggatcaa ctgaatcaat ggtagcacta tattctcgat ttggctcagc
    16501 aagggtggaa aacttaactt tttgcccgac tttaacctgt gcaatatcgc cctccgaaat
    16561 ttcaagttta atcagcatct tagataaatc tgctacctgt acaatagttg gcgaagtttg
    16621 attggaattg accgtttgcc cgacagaaac cggtacagac acaatcacgc cgtccattgg
    16681 cgagataatt tgcgtataag ttaagttggt tttagcatcg tttaccgaaa tttcagccga
    16741 ttttaattgt gctttaatgt tatccaccgt ggctttggct gaagctaagg tatttttagc
    16801 cgtttcaaga tcgtttaaag aggcactatt tgcccgatag agtttattta agcggttgta
    16861 gttcgattga gctacttcaa gtgcaacctg tgcagaggtg agctgtgctt tataagaaga
    16921 aagccgggct tgagcggtgc ttaaggtgtt gctctggttg ctggaatcaa tatctgcaat
    16981 taaatctcct tgcttcaccg attgcccgag ctcgacatag agtttttgga ttttgcccga
    17041 aacttgagcc cccacttcgg tgcgtttatt cgctcgaacc gaaccggttg ccagcacatt
    17101 tttgttaatt tctgcccgtg tgacagcttg ggtaatgtaa tggatttgcg gtttattggt
    17161 ttgttgctga taataaaaat agccgcctat tccgagagcg gcaattagca caagagtggc
    17221 taatgtttta cgtttcatac tgttccttga tgatatacaa gcggtcaaat ttagacaaaa
    17281 atttgcaaaa aagttgctct caactaaaca atgccaaatg tggctcgaag atagcttgcc
    17341 accgcttcat ctttgttaaa gccaatcact tcattattcg gtaatacggc ttttaaacgt
    17401 ggatcggcat tacccattaa acaacctttg ccggctaaac ttagcatttc cacatcattc
    17461 ataccgtcac caaaagcgat acaatcggct aaggtgtagc cccgtaattt gaccaattcg
    17521 gcgagagcat tggctttaca cactcctttt gccatcactt caaggcaaag tagggcagag
    17581 taggtcattt gcacacggtc gccataggta tcccgaatat aatgttcaat cggtactaaa
    17641 gcttcagcag ttttgccgat aaaaaacact ttctcggtat ttcgaccgtg atgatgagca
    17701 aaatttacta cttgatagct aaatcctgaa tcttgatgga atttttttaa ctctgcaatc
    17761 tctttattga taaaccattc atcgccttga tagctgttta agcacacatt ttggtcgtca
    17821 aattcaatgt tcattaattc aaaggcaaga tcttccggta aataatggtt gagaattttt
    17881 ttaccctcta aattgttggc tcttgccccg tttgaagtga ctaacattgc atttttaacg
    17941 tttactttcc caataatctg agacacatct ggatgattgc gaccggtggc gaaaaaaata
    18001 tcaacacctt tttttgatag ttgttctaat gtatcaatag tgaaatcacc aattttatga
    18061 ttggcattta atagtgtgcc atcaagatcg gaaataattg cacgaaatgg gagagtcata
    18121 atctattcct aacaataaaa t
//
LOCUS       DANLRK010000030        18102 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.030, whole genome shotgun sequence.
ACCESSION   DANLRK010000030 DANLRK010000000
VERSION     DANLRK010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 18102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18102
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.030"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(82..2271)
                     /gene="yccS"
                     /locus_tag="PXA33_000761"
     CDS             complement(82..2271)
                     /gene="yccS"
                     /locus_tag="PXA33_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HDL5120115.1"
                     /translation="MNKFLRYLQHSWLSENVIKTLPMFISLNIAAVSIWQLGISDFAM
                     PLMLGIIAGGLVDLDNSLIGRAKNLIISLVAFSLSSIGASVSLHLGWLFVPVAVVCTF
                     LLVMLGAIGQRYSTIAFGTLIVAVYNSLAYTPEVLWYHNVVMILLGTLLYGLVGILVY
                     LIFPNRTVQENLASAYLALSHYLLAKSAYFDPDDDDLAAKQLALAKANSEVMAAFDKT
                     RVALFYRLSRQHKQQRTQRMLRYYFTAQDILERASSSHYQYHELFQQLSNSDLMFRFQ
                     RVLELQAAACQKIAMSLRHREVYEHGVRGEKALQGLLNSLNYHQERGLKSAYRWRAIA
                     ENLGNIERQLAQIEQGSSSGEHIEPLSKNFTKSHRLIAENITGLSNISQAIKAHLTFE
                     SQLFRHAVRLSIVVLLCSAIVPILGFDSKGYWILLTAIFVCQPNYTATKTRLIQRVIG
                     TIFGVLVGGSFELFNVALSLEAQLGLIVLTGSLYTFFRLVHYGFSTFYITVLVMISLD
                     IAGVSLESGILMRMLDTLLGTAIAWLAVAFLWPDWKYLNLQRNLHNGLKASGQYLRHI
                     IAQLQFGYNEQLPYRIARRDVHNHLSALSNAITNMYSEPQKYANDLTFAPKLLGIAYT
                     LLGYISTLGAYRIASREINQQVDFSAMFFSHAREVANIIGQIASESRSFAKLSEKLTA
                     IDQDLTQFEQENQQKQNDLALVLIQQLRLIVQLLPQLNSILQLKSVR"
     gene            complement(2315..2773)
                     /locus_tag="PXA33_000762"
     CDS             complement(2315..2773)
                     /locus_tag="PXA33_000762"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HDL5120116.1"
                     /translation="MKTTVRTIAKQKQIALVAHDSCKQDLIRWTQTHQAQLASHKLYA
                     TGTTGHLLARETGLEITSLLSGPMGGDQQLGGLIAEKKIDLLVFFWDPMNAAPHDPDV
                     KALMRIATVWNIPVAINETTANFLLNADLFNQAISVNVPDYDGYLAERLG"
     gene            2899..4173
                     /gene="thrC"
                     /locus_tag="PXA33_000763"
     CDS             2899..4173
                     /gene="thrC"
                     /locus_tag="PXA33_000763"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HDL5120117.1"
                     /translation="MNLYNIKHPEEQVNFAQAVRQGLGKNQGLFFPEVLPKLDSIDEL
                     LDLPLVKRSQKILSALIGEELPQETLNAMVKNAFTFPAPLAKVNDDIYALELFHGPTL
                     AFKDFGGRFMAQALANVRGDGKITILTATSGDTGAAVAHAFYGLENINVVILYPKGKI
                     SPLQEKLFCTLGGNIRTVAIESDFDACQALVKQAFDDAELRQAIGLNSANSINISRLL
                     AQICYYFEAVAQLPKEKRDNVVVSVPSGNFGNLTAGLIAKTLGLPIKRFIASTNANDT
                     VPRYLQSGKWEPNATVATLSNAMDVSRPNNWPRVEELFKRNGWSLSELGSAALNDAET
                     EAALKAQYAEGYLCEPHGAIAYQSLKDQLQQGETGIFLCTAHPAKFKESVEHILAIEL
                     PLPEALDKHNKLPLLSDEMAADFATLRAYLLK"
     gene            complement(4252..4986)
                     /locus_tag="PXA33_000764"
     CDS             complement(4252..4986)
                     /locus_tag="PXA33_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HDL5120118.1"
                     /translation="MSFKDMTGQKVPSVTFRTRQNDAWVDVTTDELFNNKTVVVFSLP
                     GAFTPTCSSTHLPRYNELACEFKALGVDDIICMSVNDTFVMNAWKADQESENITVIPD
                     GNGEFTEGMGLLVDKNDLGFGKRSWRYSMLVKNGIVEKMFIEPQEPGDPFKVSDADTM
                     IKYLSPNWTAKESVSIITKPGCLFCAKAKALLKEQGYTFEEIVLGRDASTISVRAITG
                     KTSVPQVFIGGKYIGGSDELAAYFAK"
     gene            5101..5985
                     /gene="oxyR"
                     /locus_tag="PXA33_000765"
     CDS             5101..5985
                     /gene="oxyR"
                     /locus_tag="PXA33_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HDL5120119.1"
                     /translation="MNIRDLEYLIALAEHKHFRRAAESCNVSQPTLSGQIRKLEGELG
                     TILLERTSRKVLFTQAGLTLVEQAKVVLREVKILKEMASNQGKEMSGPLHVGVIPTLG
                     PYISPIIVPALKAEFADLELYIYELQTNQLLDQLESGQLDCGIISFVKESESFIEVPI
                     FNEEMYLVVPEQHEWAALKKFDMNLLKNRELLFLDSGHCLRTNTLDYCLSIGAKENPH
                     FKATNLETLRNMVAANVGLALIPKLAAKPIKGVHYIPLENPKPFRAVGMVYRPGSPLR
                     ARYERLAQAITKIMQSEA"
     gene            5988..6620
                     /gene="fabR"
                     /locus_tag="PXA33_000766"
     CDS             5988..6620
                     /gene="fabR"
                     /locus_tag="PXA33_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HDL5120120.1"
                     /translation="MANGMSVRAIQKEKTRRALVEAAFNQLSSEKNFSNLSLREVARE
                     AGVAPTSFYRHFKDMDELGLAMVDESGLLLRQLMRQARKRIANGGSVVVVSVETFFEL
                     IADRPNVFQLLLRESSGTSQAFRTAASREIQHFVAELADYIMSKDNHSNRELAYIQSE
                     GLVTLVFTAGSHALDMNNQEREKLKQRVIMQLRMLIRGSTYYANKQASST"
     gene            complement(6647..6946)
                     /locus_tag="PXA33_000767"
     CDS             complement(6647..6946)
                     /locus_tag="PXA33_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4298 domain-containing protein"
                     /protein_id="HDL5120121.1"
                     /translation="MKKADIQKMQDCYNQWVELLPELEKGIEQWKRASALLEPLSRFY
                     ASPEWRELHDSFDEQLDTKGNYSILSEDALWNALSEHHQLALEWLKLSTAYITKE"
     gene            complement(7035..10850)
                     /locus_tag="PXA33_000768"
     CDS             complement(7035..10850)
                     /locus_tag="PXA33_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HDL5120122.1"
                     /translation="MKGSGLATVGAPETGDNGQTIYTVDVAKAGAPTVTRGNVADAIN
                     NSEKTSSVVAGSKAVTVKAGKEDDKGNTEYTVDVATDKSIGRDNDGNMTVNTDNVTIV
                     KDPTTGEVKANTTTLNNSPEGKVTEPTGDDAKKLVTAGDIANAINNSGFNVTAGDGVD
                     GGETKGKKTQLIKPSETVTFDAGKNMTLTQADGKFTYTTKDNVAFNSIDMSNGKPDTT
                     GSITNLKSGVGGTFADKAAPTDAERKAIADNINNATGDTLNNAVNVGDVQAAMKAATT
                     KVEGDKGVTVTSKTNDDGSTTYTVEAKTDGTTIKVNDKGEITANTSELTNNPDGKVEE
                     PTEPNALVTAKTVADAINNAGFNIKANGDEKSLVKTGDTVQFLNGKNIEITRDGNNIT
                     VSTAKNVNFDSVQFGNEGPKITNNGGNINVGDKDGNAVKVTNVAAGDVNADSKDAVNG
                     SQLYTFAMASREEVKSTDKSVTVNTTKNADGANVFDLSVNTDDVTIVKDPTTGAIKAN
                     TTALNDANNDGRIDEPTADDAKKLVTAGDITNAINNSGFTLKTSAVEGGEKLSGGDEL
                     INPGKAVEMVAGKNLTVKQEADGKVIYATKDDVKFSSVTSNTVTVPTDEADPANNPIT
                     INKDGINAGNKAISNVASNLIPVTADDKVQPADNNPTNLADKLSNAATLGDVLNAGWN
                     LQGNGKAVDTVVHNDTVDFINGKGTTVTVENKDGKNTIKVDSPIEFVNQDPTDSSTPS
                     NTAKFTGEAPVQLGNVASSVRNEDGSTPEGKDRAEAIKNAEGDKLNNVVNLGDLQAAT
                     NAATTKVGGNRGVTITPSTNADGSTTYNVEAKTDGTTIKVDNEGNITANTSELGNNED
                     GTVKAPTQPNALLIAQTVADAVNNAGFNIKSAGNKAAGDQAATKLVKTGEEVVFEAGD
                     NLTVKRDGNQFTFATAKDVSFNSVQFSENGPKITNDGDNIKVGDKDGKPTKITNVADG
                     DISPVSTDVINGKQLNNYAKVNGNNIGTDEDGSINIVNGNGTTITSDKAGEVKVNVNI
                     TDLTVADNGKINVQDPNGTGSRFVNATTVANAVNNVSWNVDSKAVGTGAVEGDKAPAK
                     VKAGSTVSVNAGNNIKVTRKGSDVTVAVSDTPEFTSKTGDTLVNNNGVTINNGSAGKA
                     VSLTKDGLNNGGNRITNVKAGEADTDAVNVGQLKGAVNHLNNKIHRNNREARAGIAGS
                     NAAAALPQVYIPGKSMVAAAGGTFKGENALAVGYSRSSDNGKLILKLQGNANSRGDFG
                     GGVGVGYQW"
     gene            complement(10813..11298)
                     /locus_tag="PXA33_000769"
     CDS             complement(10813..11298)
                     /locus_tag="PXA33_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5120123.1"
                     /translation="MDTGSITVDNTTGAVTTPAEEDKVATTKTVSEAIQKAGWNAKSG
                     GNKADGDQEAAELINPGEKVIFAAGDNLKVKRVGTIFTYETAKDVKFDSVTFGDNGPK
                     ITNKDGNVNIAGNDGNPTKITGVKAGEADTDAVNVSQLKQAAASQNRSERFRFSNCWC
                     T"
     gene            complement(11307..13466)
                     /locus_tag="PXA33_000770"
     CDS             complement(11307..13466)
                     /locus_tag="PXA33_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HDL5120124.1"
                     /translation="MGARATSANAIALGTDTAATGNRATAFGAGALATGNRSTVMGWR
                     SAASGTRSFAMGSGAMGDKSVANATYSLALGSSANANATSTATRGTAIGYNATVSGVN
                     ATALGVSAVSSNQSTVAIGDSAKASAGFAIALGSGANVALNNSVALGSNSTVSNAVAV
                     PSATVNGITYSGFAANTTAAGNVVSVGSNTIKRQIQNVAAGQISATSTDAINSSQLYM
                     AMNATGNLANSTKNILGGNATVKPDGSVTYTNIGGTNKNTIEEALKAVKTEVVAGSNV
                     NITNATGANGQTIYTVNAYNTTANSSSPDYITVTGKAATAANTTNYEIGLTKKAIDDF
                     TKDTQATVVSNDGTVTVKSTERNANGTVIYDLSVNIPAQASQIQYFSVNSTVPENQAN
                     DGAKSRNSIAIGPNATATGGEQAAVALGTNSNANGNGALSLGVATVSKGIQATAVGHS
                     ANATANGTTALGRQTNATAGDATAVGSNANATAEKASAFGVAANASANASLAVGANSI
                     ASAQSAVAVGTRANATAQFATALGMGAQATLNSSVALGSESVVRAATPTENATVGGIT
                     YNGFAGVNKDTNYVVSVGSAGKERQIQNVAAGQISATSTDAINGSQLYMAMNATSNLA
                     NSTANNFGGGSVVNPDGSVTQPKYNVTNAAGTQYGNTATNVGDAITNLNNYVNQGFNI
                     KDNAGETKGTVTPNESVQFVNGKGTVSNVTQEADGVK"
     gene            complement(13695..13895)
                     /locus_tag="PXA33_000771"
     CDS             complement(13695..13895)
                     /locus_tag="PXA33_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ESPR domain-containing protein"
                     /protein_id="HDL5120125.1"
                     /translation="MNQIFKVIWNQATQSWVAVSELQRAKGKTKSVSSASKATLALLA
                     GASAVLGANDAVAVVSYRFNNK"
     gene            14259..15413
                     /locus_tag="PXA33_000772"
     CDS             14259..15413
                     /locus_tag="PXA33_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HDL5120126.1"
                     /translation="MNQFFPLIFSLTLFSGVTLALEKAPRTMPTEGINKPLLGQILFF
                     DNMLSHNGSQNCSSCHNPDYAFIDNRTTPAKGVVSQGDTPTKFGKRNSPTMLYAKYSP
                     EFHFDEQSQEYIGGQFWDGRAKNLAEQAGMPPIDPNEMGMPNKLEVAKRLYNVPMYQR
                     FLGQFYGEKVWESYESVYAAMEDAIATFQQENKLLAPFDSKYDLFLKGEVQLTALEEK
                     GRALFFNKAQTNCSSCHQLQSAANHQEETFSNYHYYNVGVPGNPDLIAVNGFAPDFVD
                     LGLFENPNVKGDETQKGKFKTPTLRNVAVTALYMHNGVFKELKTVLLFLDSYNNPERK
                     INPETGTAWQQAEFEPTIAHQKLKAKPLSDEEIDALEAFLKTLTDERYRK"
     gene            complement(15461..16450)
                     /locus_tag="PXA33_000773"
     CDS             complement(15461..16450)
                     /locus_tag="PXA33_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HDL5120127.1"
                     /translation="MNDLIIYNTEDGKSNVALLVVENEAWLTQNQLAELFDTSVQNIS
                     EHIKNILKDKELDENSVIKDYLITAQDGKNYQVKHYSLEMILAIGFRVRSPRAVQFRK
                     WANSTLRTYLEKGFLIDSERLKNPQGRLDYFDELLEQIREIRASELRFYQKVRELFKL
                     SSDYDKTDKNTQMFFAETQNKLIYAITKHTAAELICQRADATKMNMGLTSWKGKVVRK
                     GDIVIAKNYLDNNELDSLNRLVMIFLESAELRVKNNQDLTLGFWRRNVDMLIEFNGFS
                     VLGNGGTITHKQMESFVREQYEKFDIQRKCLKQKEADWEDLQALEKLESELTR"
     gene            complement(16610..17974)
                     /gene="mpl"
                     /locus_tag="PXA33_000774"
     CDS             complement(16610..17974)
                     /gene="mpl"
                     /locus_tag="PXA33_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HDL5120128.1"
                     /translation="MKKHIHILGICGTFMGGVAMIARELGYKVTGSDTNVYPPMSTFL
                     AAHGIEIIPNYDVAQLQPAPDLVVIGNAMSRGNPCVEYVLDNQLNYTSGPQWLHDHLL
                     KDKWVLAVSGTHGKTTTTGMLAWILDQNGIDTGFLIGGVAGNFGISARAGNSPFFVIE
                     ADEYDSAFFDKRSKFVHYTPKTLIINNMEFDHADIFDDLKAIQRQFHHLVRTMPQSGC
                     ILSAIADQNVQDTLKMGSYSQLQFIGEQNEWFAKPLNADCSQFEVFHLGVKQGEVHWS
                     IIGKHNMHNALMAIAAAHHAGVEVQGACEALGKFVNANRRLEVKGEVNGITVYDDFAH
                     HPTAIEATIDALRGKVGKNQRILAVLEPRSNTMKMGVHKDEIAPSLHDAEAVFVYQPD
                     IIAWSVSEITNVLSQPAYWSANIDDLVDLVVKEAKPTDHILVMSNGAFGGIHQKLLAK
                     LADK"
BASE COUNT         5142 a         4092 c         3369 g         5499 t
ORIGIN      
        1 tcaacaaaaa attaacaaaa ccataatatt tttattgatt ttgtgaagca tgtcacattt
       61 tatgatttaa ttataggctc actaccttac actcttgagc tgtaatatac tatttaactg
      121 cggtaataac tgcacgatca gtctgagttg ttgtatcaac actaaggcga ggtcattttg
      181 cttttgctga ttttcttgct caaattgggt gaggtcttgg tcgatagcgg tcaatttttc
      241 cgataatttt gcaaatgaac ggctctctga ggcaatttgc ccgatgatgt ttgccacctc
      301 tctggcgtgt gagaagaaca ttgcggaaaa atccacttgt tgattaatct ctcggctagc
      361 aatacggtaa gcccctaagg tggaaatata acccagcaag gtgtaagcaa tacctaacaa
      421 ttttggggcg aaggttaaat cgttcgcata tttttgtggt tcgctgtaca tattggtaat
      481 cgcattactc agtgccgata aatggttatg cacatcacgg cgggcaatac ggtaaggcag
      541 ttgctcgtta taaccaaact gcaactgggc gataatatgg cgtaaatatt gcccactggc
      601 tttcaagccg ttatgtaagt tacgttgtaa atttaaatac ttccaatccg gccataagaa
      661 tgccactgcc agccacgcaa tcgctgtacc tagtaaggta tcaagcattc gcattaagat
      721 gccgctttca aggctcacgc ctgcaatatc aaggcttatc attaccaaca cagtgatata
      781 aaaggttgaa aagccataat gtaccaagcg gaaaaaggta taaagcgaac cggtcagtac
      841 gattagccca agctgggctt cgaggctgag cgccacgtta aacagttcaa aagagccacc
      901 aaccagcacg ccgaaaattg tgccgatcac acgctgaatc agtcgggtct tagtagcagt
      961 gtaattcggt tggcagacaa aaatggctgt gagtaaaatc cagtagcctt tgctatcaaa
     1021 gcctaaaatc ggcacaatcg cactgcacaa cagcaccact atcgacaacc gaacggcgtg
     1081 tcgaaacagt tgggattcaa aggttaaatg ggctttaatt gcttggctga tattgcttaa
     1141 gccggtaata ttttcggcga tcaagcggtg agattttgtg aaatttttac ttaacggttc
     1201 aatatgctca ccactgcttg agccttgttc aatctgggct agttgccgct caatattgcc
     1261 taaattttct gcaatagcac gccagcgata agcacttttc aagccccgtt cttgatgata
     1321 attcaatgaa ttaagcaagc cctgcaaggc tttttcgcca cgaacgccgt gttcatatac
     1381 ttctctatgg cgtaacgaca ttgcaatttt ttgacacgcc gcagcctgta attccagcac
     1441 ccgttgaaag cggaacatta aatcactgtt acttaattgt tggaacagtt cgtgatattg
     1501 ataatggctg gaacttgccc gctctaaaat atcttgagcg gtgaaatagt aacgcaacat
     1561 tcgctgtgtc cgttgctgct tgtgctggcg gcttaggcgg tagaataggg caactctggt
     1621 tttatcaaag gcagccatca cctcactatt ggctttcgcc aaggctagtt gtttggcagc
     1681 taaatcgtca tcatcagggt caaaataggc agatttcgcc agtaaataat ggctcaacgc
     1741 aaggtaggcg gacgctaaat tctcctgcac ggttcgattc gggaaaatca ggtacaccaa
     1801 aataccgacc aaaccataga gtaaggtgcc aagtaagatc atcaccacat tgtgatacca
     1861 caatacttcg ggcgtataag ccaggctgtt ataaaccgcc acaatcagcg tgccaaaagc
     1921 aatggtacta tagcgttgcc cgattgcccc taacattacc agcaaaaagg tgcaaaccac
     1981 cgccaccggc acaaacagcc agcccaaatg gagcgacaca ctcgccccaa tcgaggaaag
     2041 cgagaacgcc actaggctga taatcagatt tttcgctcgt ccgattaggc tgttgtctaa
     2101 atccaccaaa ccgccggcga taatgcctaa catcaacggc atcgcaaaat cagaaatacc
     2161 gagctgccaa atgctgaccg ccgcaatgtt tagcgaaata aacatcggca gggttttaat
     2221 tacattttcc gaaagccaac tgtgttgtag atatcttaaa aatttattca tatttgctca
     2281 tttcgggcag gcacaggacc tgcccctacc tacactaccc caaacgttct gccaaatagc
     2341 catcataatc cggcacattc acgctgattg cttgattgaa caaatccgca tttaacagaa
     2401 aattagcggt ggtttcgtta atcgccaccg gaatattcca aacggtcgca atccgcatca
     2461 gggctttcac atcaggatcg tgcggagcgg cattcatcgg atcccaaaag aacaccagca
     2521 gatcaatttt cttttctgcg atcagcccgc ctaattgctg atcgcccccc atcgggccgc
     2581 ttaacaatga agtaatttca agccctgttt ctctggcgag caaatgcccg gtagtgccgg
     2641 tggcatatag cttatgcgag gcaagttggg cttgatgagt ttgtgtccaa cgaattaaat
     2701 cttgtttaca gctatcgtga gccactaagg caatctgttt ttgttttgcg atggttctga
     2761 cggtggtttt catttttagc tcctttgcaa aatttttcac aaatcatacc gcttattttt
     2821 cgcttttgca ttgactaatc acggcttatg gctaaaatca ggtaaaaatt cgcattttaa
     2881 aacaaaggat taaccgcaat gaacttatac aacatcaaac accctgaaga acaagtcaat
     2941 ttcgcccaag cggtacgcca aggcttaggc aaaaatcaag ggctattttt ccctgaagtg
     3001 ttacctaaat tagatagcat tgatgaatta ttggatttac ctttggttaa acgcagccaa
     3061 aaaattcttt cagccttaat cggcgaagag ttaccgcaag aaaccttaaa tgcaatggta
     3121 aaaaatgcgt tcactttccc ggcaccgctt gccaaagtca atgatgatat ttatgcgtta
     3181 gaattatttc acggtccgac tttagccttc aaagattttg gtgggcgctt tatggctcaa
     3241 gccttagcca atgtacgagg agacggcaaa attactattt taaccgctac ctcaggtgac
     3301 accggtgcgg cggtggctca tgctttctat ggtttggaaa atatcaacgt ggtcattctt
     3361 tacccgaaag gcaaaatcag cccattacaa gaaaaattat tctgtacctt aggcggtaat
     3421 attcgcactg ttgcgattga atccgacttt gatgcttgcc aagccttagt gaaacaagcc
     3481 tttgatgatg ccgagctacg ccaagcgatt ggtttaaatt ccgcaaattc catcaacatt
     3541 agccgtttac tggctcaaat ttgctactac tttgaagcag ttgctcagtt accaaaagaa
     3601 aaacgagata acgtagtggt ttcagtacca agcggtaatt tcggtaactt aaccgccggc
     3661 ttaattgcca aaacattagg tttaccgatt aaacgtttta ttgcctcaac caatgccaat
     3721 gacaccgtgc cacgctattt acaatcaggc aaatgggaac caaacgcaac cgttgcaacc
     3781 ctttctaatg caatggacgt aagccgcccg aacaactggc cacgagttga agaattattt
     3841 aaacgcaatg gctggagttt gtctgaattg ggttctgccg cattaaacga tgccgaaacc
     3901 gaagcggctt tgaaagctca atatgcagaa ggttatctgt gtgagcctca cggtgctatt
     3961 gcttaccaaa gcttgaaaga tcagctacaa caaggcgaaa ccggtatttt cttatgcacc
     4021 gctcacccgg caaaattcaa agagtcggtg gaacacattt tagcgattga attgccgtta
     4081 ccggaagcat tagataagca caacaaactg ccactgttat ccgatgaaat ggcagcagat
     4141 tttgcaacat tgcgtgcata tttgttgaaa taaatattca ataatagaaa agggattaag
     4201 tattaaactt aatccctctt tttatgcatt acaagcggtt atatttgcct attattttgc
     4261 aaaataagca gctaactcat cactaccgcc aatatattta ccgccgataa atacttgtgg
     4321 cacagaggtt ttacctgtaa ttgctcgtac agaaattgtg cttgcatcac gacctaacac
     4381 aatctcctca aaagtatagc cttgctcttt taataatgct tttgcttttg cacagaacag
     4441 gcaacccggt ttagtgataa tcgaaacaga ctctttagct gtccaatttg gacttaaata
     4501 tttaatcatt gtatcggcat ctgatacttt aaacggatcg cccggttctt gtggctcaat
     4561 aaacattttt tccacaatac cgtttttcac cagcatcgaa taacgccaag aacgtttacc
     4621 aaacccaagg tcatttttat caactaatag ccccatacct tctgtgaact caccattacc
     4681 atcaggaata acagtaatat tttcagattc ttgatctgct ttccaagcgt tcattacaaa
     4741 ggtatcatta actgacatac agatgatatc atctacccct aatgccttaa attcacaagc
     4801 taattcatta tagcgaggca aatgtgttga tgaacaagtc ggtgtaaatg ctccgggtaa
     4861 tgaaaacaca accaccgttt tgttattaaa taactcatcg gttgtgacat caacccaagc
     4921 atcgttttga cgtgtacgaa atgttacact gggtactttc tgacccgtca tatctttaaa
     4981 agacatatta atgttctcct atagaattat atattacaaa aatcttaggc attatactca
     5041 caaatatggc ataattcagc ctatcagaac aataggaaaa atttaaattg gaggaatcaa
     5101 gtgaatattc gagatttaga ataccttatt gcacttgcag aacataagca tttccgtcgt
     5161 gcagcagaat cttgtaatgt tagccaacct actttaagcg ggcaaattcg caaattagaa
     5221 ggcgaactag gcactattct gcttgaaaga accagtcgca aagtcctctt tacgcaagca
     5281 ggcttaacat tagttgaaca agccaaggta gttttacgtg aagtaaaaat tctaaaagaa
     5341 atggcaagca accaaggtaa agagatgtct ggcccgcttc atgttggtgt aatcccaacc
     5401 ttggggcctt acatttcgcc aattattgtg cctgcattaa aagcagaatt tgcagattta
     5461 gagctgtata tttacgaatt acaaaccaac caattattag atcaacttga atccggccag
     5521 cttgattgtg gcattatctc atttgtaaaa gagagtgaat cttttattga agtgcctatc
     5581 tttaatgaag aaatgtactt ggtagttcca gaacaacacg aatgggcagc gcttaaaaaa
     5641 ttcgatatga atttattaaa aaaccgtgag ttgctctttt tagacagtgg ccattgttta
     5701 cgcactaata cgctagacta ttgtctatcc attggagcaa aagaaaatcc acactttaaa
     5761 gctaccaacc ttgaaacctt acgcaatatg gtagcagcaa atgttggcct agcattgatt
     5821 cctaaattag ccgctaaacc gattaaagga gtacattata ttccacttga aaacccgaaa
     5881 ccgtttcgag ctgtgggaat ggtgtatcga ccaggttcac cattacgcgc ccgctatgag
     5941 cgtttagctc aagccattac taaaattatg caatccgagg cgtaaaaatg gcaaacggta
     6001 tgagtgtgcg cgcaattcag aaagaaaaaa cacgcagagc attagtagaa gccgccttta
     6061 atcaattaag ctcagaaaaa aatttctcta atctcagttt gcgtgaagtt gcacgtgaag
     6121 ctggagtcgc accgacttcg ttttaccgcc actttaaaga tatggacgaa ctaggcttag
     6181 caatggtgga cgaatccggg ctactattac gccaattaat gaggcaagct cgaaaacgaa
     6241 ttgcaaatgg cggaagtgtt gtcgtggttt cagtggaaac attcttcgaa ctgattgccg
     6301 accgccctaa cgttttccag ctattactac gcgaaagctc cggtacatcg caagcgttta
     6361 gaaccgcagc atcacgcgaa attcaacatt ttgtagctga attggcggac tatattatga
     6421 gcaaagataa tcacagtaac cgagaacttg cttatattca atcggaaggt ttagtcacgc
     6481 tagtatttac tgccggttct catgctttgg atatgaacaa tcaagaacgg gaaaaactaa
     6541 agcaacgtgt cattatgcag ctaagaatgc tgattcgtgg ctccacctat tatgccaata
     6601 aacaagcttc atcaacataa aaaaacagac gcctaagcgt ctgtttttat tctttcgtaa
     6661 tatatgcagt cgatagcttt aaccattcta gtgctaattg atgatgttca gataacgcat
     6721 tccataacgc atcttctgac aagatactat aattgccttt agtatctaac tgttcatcaa
     6781 aactatcatg taattcacgc cattccgggc tagcataaaa ccgacttaag ggttccaaaa
     6841 gtgcagaagc tcgtttccat tgctcaatcc ccttttccaa ttctggtaat agctctaccc
     6901 attggttata acaatcctgc attttctgaa tatctgcttt tttcattctt taccgcttac
     6961 accataaatt ttagcctcaa tataacataa aaatcccctt ccgaagaagg ggatttcaac
     7021 tcaattaggc taaattacca ctggtatcca acgcctacac cgccaccaaa gtcgcctcgg
     7081 ctattcgcat taccttgtaa tttcaagata agcttaccgt tatcacttga acgtgaataa
     7141 cctactgcaa gcgcgttttc acctttaaat gtaccacctg ctgcagccac catagattta
     7201 cctggaatgt aaacctgcgg taacgctgct gctgcatttg aaccggcaat accagcacgg
     7261 gcttcacggt tattacgatg gatcttgtta tttaagtgat taacagcacc ttttaactga
     7321 ccaacattaa cagcatcggt atctgcctca cctgctttaa cattagtaat acggttacca
     7381 ccattattta agccatcttt cgttaagctt accgcttttc ctgcagagcc attattaatg
     7441 gttacgccat tattgttcac caatgtatca ccagtcttgc tagtaaattc aggggtatct
     7501 gaaacggcta cagttacatc agaaccttta cgtgtcactt tgatattatt acctgcattc
     7561 acgctcactg tactacctgc ttttactttc gcaggggctt tatccccttc aaccgcacct
     7621 gtaccaactg ctttagaatc tacgttccag cttacgttgt ttacggcatt tgctactgtt
     7681 gttgcattca caaagcgtga accggtaccg ttaggatctt gaacattgat tttgccgtta
     7741 tcagcaacgg ttaaatctgt gatgtttacg ttcactttca cctcaccagc cttatcactg
     7801 gtaatagttg taccattacc atttacgata ttgatagaac catcttcatc agtaccaatg
     7861 ttattaccat tcactttcgc atagttgttc agctgtttac cgttgataac atctgtgctt
     7921 actgggctaa tatcgccatc tgctacatta gtgatcttag ttggtttacc gtctttatca
     7981 ccgactttga tgttatcacc atcattagtg attttcggac cattctcact gaactgtacg
     8041 ctattgaagc tcacatcttt tgctgtcgca aaagtaaatt gattaccatc acgtttaaca
     8101 gttaagttat cacctgcttc aaacacaact tcttcgcccg ttttcactag cttagtcgca
     8161 gcttggtcac cggcagcctt attacctgca gatttaatat taaagcctgc attattcact
     8221 gcatctgcaa cagtttgagc aatcaataac gcatttggct gagttggtgc tttcaccgta
     8281 ccatcttcgt tgttacctaa ctcactggta tttgcagtga tgttaccttc attatctact
     8341 ttaatagtag taccgtcagt tttcgcttca acgttataag tagttgaacc atcagcattc
     8401 gtgctaggtg tgatagttac acctcggtta ccgcccactt tcgtagtagc tgcattagtt
     8461 gccgcttgta aatcacctaa gttcactacg ttgtttagct tgtcgccttc agcattttta
     8521 atcgcttctg cacggtcttt accctctggg gttgagccat cttcgttacg aacactactt
     8581 gccacattgc ctaattgaac aggggcttca cctgtgaatt tagccgtatt gcttggcgtt
     8641 gagctatcag taggatcttg gtttacaaac tcaatcgggc tatctacttt aatcgtattc
     8701 ttaccgtctt tgttttcaac cgtgaccgtt gtacctttac cgttaataaa atcaaccgtg
     8761 tcattatgta ccacagtatc aaccgctttg ccgttacctt gtaaattcca gcctgcattt
     8821 aatacatcac ctaatgttgc tgcattactt aacttatcag ctaagttggt tggattattg
     8881 tctgctggtt gaaccttatc atctgcagtc acaggtatca gattagacgc aacattgcta
     8941 atcgctttat tacctgcatt gataccatct ttattgatag taattggatt atttgcagga
     9001 tctgcttcat cagttggtac agttacagtg ttagaggtta cgctgctaaa cttaacatca
     9061 tctttagtcg cataaatgac tttaccatca gcttcttgct tcacagttaa gtttttacct
     9121 gcaaccattt caactgcttt acccggatta atcagttcat caccacctga aagcttctct
     9181 ccaccttcta cagccgacgt tttcaacgta aagcctgagt tattgatggc gttggtaata
     9241 tcacctgctg tcactaattt cttagcatca tcagcggttg gttcatcaat tctaccgtca
     9301 ttatttgcat catttaatgc cgtagtgtta gccttgatcg caccggttgt aggatctttt
     9361 acaattgtta catcgtccgt attcacagaa agatcgaaca cattcgcgcc atcagcattt
     9421 tttgtcgtgt taaccgtaac tgatttatca gtcgacttca cttcttcacg agatgccata
     9481 gcgaaagtat aaagctgact accatttact gcatctttac tatctgcatt tacgtcaccg
     9541 gcagctacat tagttacttt gactgcatta ccgtctttgt caccaacatt gatattacca
     9601 ccattgttag taatcttagg accttcattt ccgaactgta cactatcaaa attcacattt
     9661 tttgcagttg aaacagtaat gttattccca tcacgggtaa tctcaatgtt tttaccattt
     9721 aagaactgaa ccgtatcacc tgttttcacc aggctcttct cgtcaccgtt tgctttaatg
     9781 ttaaaacctg cattgttaat tgcatctgca acggttttag ctgttactaa tgcatttggt
     9841 tctgtaggct cttccacttt accatcaggg ttatttgtta actcgctggt gtttgccgtg
     9901 atttcgccct tatcgtttac cttaatggtt gtaccatccg tttttgcttc aacggtataa
     9961 gtggttgagc cgtcatcgtt agttttagat gttacagtaa cacctttatc accttccact
    10021 ttggtggttg ctgctttcat tgctgcttgt acatcaccca cattcacagc attattcaac
    10081 gtatcacctg ttgcgttgtt aatattatcc gcaatcgctt tacgctctgc atctgttggt
    10141 gctgctttat ctgcaaatgt accacccacg ccgctcttca gattagtgat tgagcctgtt
    10201 gtatctggtt taccattgct catatcaatg ctgttgaatg caacattatc tttcgttgtg
    10261 taagtgaatt taccgtcagc ttgggtaagt gtcatatttt tacccgcatc aaaggtaact
    10321 gtttcacttg gcttgattag ctgtgttttc ttacctttgg tttcaccacc atcaacacca
    10381 tcacctgctg ttacattaaa gcctgaatta ttgattgcat tagcaatatc acctgccgtc
    10441 actaatttct tagcatcgtc acctgtaggc tcagtaactt taccttcagg agaattattt
    10501 aatgttgtgg tattcgcttt aacttcgcct gttgtcggat ctttcacgat agtaacgttg
    10561 tcagtattca ctgtcatatt accgtcatta tcacgaccga tagatttatc cgttgcaaca
    10621 tctaccgtat attcagtatt gcctttatca tcttcctttc cggctttaac agttacggct
    10681 ttagaaccag ctactacaga tgaggttttc tctgaattat taattgcatc agccacatta
    10741 ccacgagtta ctgtaggagc acctgctttc gctacatcta ccgtatagat tgtttgacca
    10801 ttatcacctg tttcaggtgc accaacagtt gctaaacctg aacctttcac ttcggttttg
    10861 tgacgctgct gcttgtttta actgacttac attcaccgca tcagtatcag cttcacctgc
    10921 tttcacgcct gtaattttgg ttggattacc atcattacct gcaatattaa cattaccatc
    10981 tttattcgtg attttcgggc cattatcacc aaatgttacg ctatcaaatt tcacatcttt
    11041 cgctgtttcg tacgtgaata tagtgccaac acgtttaact tttaagttat cgcctgctgc
    11101 aaaaatcact ttttcgcccg gattaatcaa ctctgctgct tcttgatcac catcagcttt
    11161 attgccacca gattttgcat tccaacctgc tttttgaatc gcttcagaaa ctgtttttgt
    11221 agttgcaact ttatcttctt ctgcaggtgt agttactgca cctgttgtgt tatcaaccgt
    11281 gatagaaccg gtatccacat caaaggttac tttacgccat cagcttcttg tgtaacattt
    11341 gaaactgtac ctttaccgtt tacaaattgt acagattcat ttggggtcac tgtgcctttg
    11401 gtttcacccg cattatcttt aatattgaaa ccttggttta catagttatt taagttcgta
    11461 attgcatcgc caacgtttgt tgcagtatta ccatactgag ttccagctgc attcgttaca
    11521 ttatatttag gctgagtcac agagccatcc ggattcacaa cagaaccgcc accaaaatta
    11581 tttgcagttg aatttgcaag gttactggtt gcattcattg ccatataaag ttgtgaacca
    11641 ttaatcgcat cggttgatgt agcagaaatc tgacccgctg ccacattttg gatttgacgc
    11701 tctttacctg cagagcctac agaaacaaca tagttagtat ctttatttac cccggcaaag
    11761 ccattataag taataccccc taccgttgca ttttcagttg gtgttgctgc ccttacgact
    11821 gattcgctac ccaaagcaac agaactattc agagtagctt gagctcccat acctaatgct
    11881 gtagcaaatt gagctgttgc attagcacgt gtaccaacag ccactgctga ttgtgctgag
    11941 gcaattgaat ttgcacctac ggctaaagag gcatttgcac tcgcatttgc tgcaacacca
    12001 aaggcagaag ctttttcagc cgttgcatta gcattagaac ctaccgctgt agcatcacct
    12061 gctgtcgcat ttgtttggcg accaagtgcg gttgtaccat tagccgttgc atttgctgaa
    12121 tgaccaactg ccgtagcttg aatacctttt gaaaccgtag caacacctaa agataacgct
    12181 ccgttaccat ttgcatttga gtttgtgcct aatgctactg ccgcttgctc tccacctgtt
    12241 gccgttgcat tcgggccgat cgcaattgag tttctgctct tcgcgccatc attagcttga
    12301 ttctcaggta cggttgaatt cacagagaaa tattgaattt ggcttgcttg cgcaggaata
    12361 ttgacagaaa gatcataaat aaccgtgcca tttgcatttc tctctgtact ttttacagtt
    12421 actgtaccat cgtttgaaac aacagtagct tgggtatctt tcgtaaagtc atcaatggct
    12481 ttcttagtta aaccaatttc atagttagtt gtattcgcag cagttgcagc tttacctgtt
    12541 acagtaatgt aatcagggct agaagaattt gccgtggtgt tatacgcatt tactgtataa
    12601 atagtttgcc catttgcacc ggttgcattt gtaatattaa cattgctacc tgcaacaact
    12661 tctgttttta ccgcttttaa tgcttcttca atcgtatttt tatttgttcc accaatatta
    12721 gtataggtaa cagaaccatc aggctttact gtagcattac cacctaaaat atttttagtt
    12781 gaattagcaa ggttacctgt tgcgttcatt gccatataaa gctgtgaact attaattgca
    12841 tcggttgatg tagcagaaat ctgacccgct gccacatttt ggatttggcg tttgatcgtg
    12901 tttgagccaa cagaaacaac attccctgct gccgtagtat tagccgcaaa gccggaataa
    12961 gtaataccat ttaccgtcgc agaaggaact gccacagcgt tggaaacggt tgaattactc
    13021 cctaaagcaa ccgaattatt tagcgctaca ttagcacctg agcctaaggc aatagcaaaa
    13081 ccggcacttg ctttagcact atcaccaatt gcaaccgtag attggttgga agatactgca
    13141 cttaccccta aagctgtcgc attaacacct gaaactgtcg cattataacc aatcgctgta
    13201 cctctagttg cagtagaagt tgcattagca ttagcacttg aacctaaagc taaagaataa
    13261 gtagcatttg caacagactt atcccccatt gcacctgaac ccattgcaaa tgaacgagtg
    13321 ccactagcgg cagatctcca tcccattact gttgaacggt taccagtagc tagagctcct
    13381 gcaccaaaag ctgttgcacg gttacctgtt gcagcagtat ccgtacctaa tgcaatagca
    13441 tttgctgaag tcgctctagc ccccatacca atagcaatag aaggaactaa tgcccaattc
    13501 tgaccagtgt tattgagact ctctacacca atattatagt aatgtaagaa gtatcttctg
    13561 gatccactaa atttgttcta tgcgttgcat tcgtatctgt accaattgca atagtagatc
    13621 tgccagttga gattgaatcc gcaccaatag caattgcgtt ctccgatgat gcagttgctt
    13681 gtgttgaaat ataattactt gttgttgaac ctataagata caacagccac agcatcatta
    13741 gctcccaaca cggcactagc tccagccaat aaagccaatg ttgccttact tgcagaacta
    13801 acagatttcg ttttaccttt cgctcgttgt aattcagata ctgcaaccca actttgtgtt
    13861 gcctgattcc aaataacttt aaaaatttga ttcataatcg accctatata aaaataaaaa
    13921 agattacgta gaacctatgt tcaacataat cttcaccaat ataaaaaacc gccacctatt
    13981 taagtgaaat aacttactta tagactcaga aatttaagtg cgcgtatttt acgctatttg
    14041 taatataatt catcttctgg agtgtaaatt tattgtaaat aatacgagga atctaaccgc
    14101 ttgttataac gcaaaacacc ttgacttaaa tcaagccact acctcatttt gaggggagat
    14161 ttatacgcag tgaaatttta aaataagcta aggtttacgg ataatcgttc aacactattg
    14221 aattatcact gtagttatcg ttaaaaaggt atgcttttat gaaccaattc ttccctctaa
    14281 tctttagctt aactctattt tccggcgtaa ctttggcact agaaaaagca cctcgaacta
    14341 tgccgacaga aggaataaat aagcccctac ttggacaaat tctattcttt gacaatatgc
    14401 tgtcacacaa cggttcacaa aactgctcca gctgccataa tccggattat gcctttattg
    14461 ataaccgcac tactccggca aaaggggtgg tttcacaagg agatactcca accaaattcg
    14521 gcaaacgcaa ctccccgacc atgctttatg cgaaatactc gcctgagttc cactttgatg
    14581 agcaaagtca agaatatatc ggcgggcagt tctgggacgg acgagccaaa aatctcgccg
    14641 agcaagccgg tatgccgcct atcgacccaa atgaaatggg aatgcctaac aaattagaag
    14701 tagccaaaag attatataat gtgccgatgt atcagcgatt tttagggcaa ttttacggtg
    14761 aaaaggtatg ggaaagttat gaaagcgttt atgcggcaat ggaagatgcg attgcgacat
    14821 tccaacaaga aaacaaactt ctcgctccat ttgattcaaa atatgatcta tttttaaaag
    14881 gcgaggtaca attaactgca ttggaagaaa aaggcagagc tttatttttc aataaagctc
    14941 aaactaattg tagtagttgc caccaattac agagtgctgc aaaccaccaa gaagaaacct
    15001 ttagcaacta tcactattac aatgtaggcg tacccggtaa cccagacttg atagcggtta
    15061 acggcttcgc accagacttc gtcgatttag gcttatttga aaacccaaat gttaaaggtg
    15121 atgaaacaca aaaaggcaaa ttcaaaaccc caacattgcg taatgttgcc gtgactgcac
    15181 tttatatgca caacggtgtg tttaaagaat taaagactgt tttgctgttt ttagacagct
    15241 acaataaccc ggagcgaaaa atcaaccctg aaaccggcac ggcgtggcaa caagcagaat
    15301 ttgaacccac tatcgcccac caaaaactca aagccaaacc cttaagcgat gaagaaattg
    15361 atgccctcga agcattttta aaaaccttga cggatgagcg gtatagaaaa taaggctaca
    15421 agcggtaaaa ttttggggaa attttaccgc ttgcgtttaa ctaccgtgtt agctcacttt
    15481 ctaacttttc caatgcctgc aaatcttccc aatctgcctc tttctgtttt aaacatttac
    15541 gctgaatatc aaatttttcg tattgctctc tgacaaagct ttccatttgt ttatgcgtga
    15601 ttgttccacc atttcctaat acagaaaagc cgttaaactc aatcaacata tctacatttc
    15661 ttcgccagaa acctaacgta agatcttggt tgttcttcac acgtaattcg gcactttcaa
    15721 gaaagatcat taccaagcga tttaaagaat ccagctcatt attatctaaa tagtttttcg
    15781 caatcacaat atcgccttta cgcacgactt ttcctttcca gctagttaat cccatattca
    15841 tcttagtcgc atcagcacgt tgacaaatca actcagcggc agtgtgtttg gttattgcat
    15901 aaatgagctt attttgcgtt tctgcaaaaa acatttgcgt atttttatct gttttgtcgt
    15961 aatcgctaga gagtttgaaa agctctcgta ctttttgata gaaacgcaat tcacttgccc
    16021 gaatctcacg aatctgttca agtaattcat cgaaataatc taaacgaccc tgcggatttt
    16081 tcaaacgctc gctatcaatt aaaaaacctt tttcgagata agtgcgtaat gtgctgtttg
    16141 cccattttct aaactgtact gctcttgggc tacgaactcg aaaccctatc gccagaatca
    16201 tttcaagtga ataatgtttt acttgataat tcttgccatc ttgagcagtt atcaagtaat
    16261 ccttgataac tgaattctca tctaactctt tgtcttttaa gatattttta atgtgttcgg
    16321 atatattttg cacagaggtg tcaaaaagtt ctgcaagttg attttgtgtc agccaagctt
    16381 cattttcaac cactaacaag gctacattgc ttttgccgtc ttccgtatta taaataatta
    16441 aatcgttcat attttattct gtttattggt agagatatta gtcgctattt tcaataaaac
    16501 atgtataaat atccagaatt gattttgaat ttttcgatgc agatcacaaa aatagagagt
    16561 tatacaaaca agcggtaaaa ttttgtaaaa aatttaccgc ttgtaaaggt tacttatcag
    16621 ctaatttggc aagtagtttt tgatgaatac cgccaaaggc tccgttgctc atcactaaga
    16681 tgtgatcggt gggtttggct tctttgacga ctagatcgac taaatcatca atatttgccg
    16741 accaatacgc cggttgcgat aacacattgg taatctcgct cacactccac gcaataatat
    16801 caggctggta aacaaacact gcttcggcat cgtgtaggct tggggctatt tcatctttat
    16861 ggacacccat tttcattgta tttgaacgag gctctaacac tgccaaaatg cgttgatttt
    16921 tgccgacttt accacgcaga gcatcaatag tcgcttctat cgcagttggg tggtgagcga
    16981 aatcatcata aacggtaatg ccgtttacct cgcctttcac ttccaaacgg cggttagcat
    17041 tcacaaactt gcctaatgcc tcgcaagccc cttgcacttc aacccctgcg tgatgagcgg
    17101 cggcaatcgc catcagagca ttatgcatat tgtgcttgcc gataatcgac caatgcacct
    17161 cgccttgttt aacgcctaag tgaaacacct caaactgtga gcaatcggca tttaacggtt
    17221 tagcaaacca ttcgttctgt tcgccaataa attgaagctg gctgtaactg cccattttta
    17281 gtgtatcttg cacgttttga tcagcgattg ccgacaaaat acagccactt tgtggcatcg
    17341 tgcgaacaag gtggtggaac tggcgttgaa tcgcttttaa atcatcaaaa atatcggcgt
    17401 ggtcaaattc catattattg ataatcagcg ttttcggggt gtaatgcaca aatttagaac
    17461 gcttatcaaa gaacgcagaa tcgtactcgt ccgcctcaat cacgaagaat ggactgttac
    17521 cggcacgagc cgaaatgccg aaattaccgg caacaccgcc aattaaaaag ccggtatcaa
    17581 tcccattttg gtctaaaatc cacgctaaca ttcctgttgt ggtagttttg ccgtgcgtgc
    17641 cggaaaccgc cagcacccat ttgtctttaa gcaaatgatc gtgcaaccat tgcgggccgg
    17701 aggtgtaatt gagttggttg tccaacacat attccacgca cggattacca cggctcatcg
    17761 cattaccaat caccactaaa tcaggagccg gttgcagttg agcaacatcg taattcggga
    17821 taatttcaat accgtgagca gctaaaaagg tactcatcgg tgggtaaaca ttagtgtctg
    17881 agccggtcac tttatagcca agctcacggg caatcattgc cacaccgccc ataaaggtgc
    17941 cgcaaatgcc taaaatatgg atatgttttt tcatcttttt atatttaaaa tgttagaatc
    18001 atcccctctc cccttgtggg agagggatag attttttctt cgtgcagaag aaaaaatcag
    18061 ggagaggggt aagcggtcat ttttaggctt aatattgtaa gc
//
LOCUS       DANLRK010000031        17996 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.031, whole genome shotgun sequence.
ACCESSION   DANLRK010000031 DANLRK010000000
VERSION     DANLRK010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 17996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17996
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.031"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            70..1161
                     /gene="ulaG"
                     /locus_tag="PXA33_000775"
     CDS             70..1161
                     /gene="ulaG"
                     /locus_tag="PXA33_000775"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HDL5120129.1"
                     /translation="MSKIQEITRESWILSTFPEWGTWLNEEIEEEVVPEGNFAMWWLG
                     CVGVWIKTPAGANICMDLWCGRGKSTKKVKDMVRGHQMANMAGVRKLQPNLRAQPMVL
                     DPFAINEVDFILASHYHSDHIDVNVAAAICNNPKLDHVKFVGPWHCAELWKKWGVPEE
                     RIIVVKPGDVVKLKDVEIHALDSFDRTCLVTLPVEGAEERGGELAGLCPSDEEMGRKA
                     VNYVFKTPGGTIYHGADSHYSIQFAKHGKQFDIDVALNNYGENPVGIADKMTSVDLLR
                     MAECLRCKVIIPVHHDIWTNFMASTQEIIDLWRMRKDRLQYQFHPFIWEVGGKYVYPR
                     DKDLIEYHHPRGFDDCFEQEPNIQFKSML"
     gene            1245..1991
                     /gene="ulaR"
                     /locus_tag="PXA33_000776"
     CDS             1245..1991
                     /gene="ulaR"
                     /locus_tag="PXA33_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HDL5120130.1"
                     /translation="MNESYRHKELLALLEERKALSTADIMQLLNISPATARRDISKLD
                     GQGKLKKVRNGAEAILAPTFLQHTKITNNAQEKRRIAEAAGRLCKDGESLILTCGSTM
                     EMLGEVLCGRKLQIITNYLPLANQLIAGCHDDVVIMGGQYNKHKKVMLSINTQNEMTY
                     AATTMFTSGKGLTSEGLFKTDMIIANAEQQMAKHAERYVVLLDSTKLGKKVGMLFKEF
                     DKIDLLITGKEADPTIIQALRNKGLDIILA"
     gene            2046..2906
                     /locus_tag="PXA33_000777"
     CDS             2046..2906
                     /locus_tag="PXA33_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HDL5120131.1"
                     /translation="MRKHKLGIYEKALPKNISWQDRLSIAKVCGFDFVEMSIDETDER
                     LARLDWSKKERAKLVKTIINTGVTIPSMCLSGHRRFPFGSCDEATRQKAYEIMEKAIQ
                     LAVDLGIRTIQLAGYDVYYEEQDEGTIQRFQQGLEWAVELAASNQVTIAVEIMDTQFM
                     SSISRWKKWDEIIKSPWFTVYPDLGNLSAWNDNVAEELKLGIDKISAIHLKDTYKVTE
                     TCKGQFRDVPFGDGCVDFAACFKTLAELNYRGAFLIEMWTEKAEEPITEIINARRWIE
                     QKMQEGGFTL"
     gene            2903..3622
                     /locus_tag="PXA33_000778"
     CDS             2903..3622
                     /locus_tag="PXA33_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDL5120132.1"
                     /translation="MTQLTELTPALQALRERVLKANLELPKYGLVTFTWGNVSEIDRE
                     TGLVAIKPSGVEYDAMTVEDIVLVDLQGNRVYGDKKPSSDTETHLELYRQFTDIGGVV
                     HTHSRNAVGWAQAGRDLLALGTTQGDYFYGAIPCTRKMTAEEIAGSYELETGKVIVET
                     FRTRGIEPKAVPGVLVHSHGPFTWGKDGHDAVHNSVVLEEVAYMNFISHQIRPQIGPM
                     QQELLDKHYLRKHGANAYYGQ"
     gene            3843..6320
                     /locus_tag="PXA33_000779"
     CDS             3843..6320
                     /locus_tag="PXA33_000779"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012311.1,HMM:TIGR02601.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HDL5120133.1"
                     /translation="MYLLEGNQKVKNRNYFVAIATILALSPEVNATELSDQLASRTRY
                     YPSATDSYYSYYKYAPIYSGYYTSNSRNGYSSWYYPGSNAGVTTRTVSTTVNGITVTK
                     VYTTHNGRTTVRTTYAPAVNKPIVNKPTPIANKPVVDTPTQPVIDANPIMDPPVQPIV
                     DNKPITDTQPNPITDNKPVIESPIDDTRPVIIRPTTPVNLHPEPVAVGVPNVGIRWND
                     RSRGYNIDDPNNKQNVKLTGEGVKVGILDSGFKNYLMQDDILKKFGNRAVIIETGRTA
                     PASATHGIEVAEMVGGGNSRNGVASKSTLLLADITKTTNAGTGLSATAPIYYDLWNRG
                     ARIFNQSYGIPKPLTYFNNNRNSSYYYLHQFDGNVLQFYKDKVKEGGLFVWAAGNNRG
                     DLDPSLQAGLPHYEADLEKGWISVVAVATRTNQGLGNFEWSNLLPYSQAGVAKNWTVT
                     AMGDYIFNLGGRNIIASGSSFAAPAVTGTAALVKQKYPWMDGNLIKQSILSTATDIGA
                     PGVDDVYGWGLLNIEKATNGPARFGKRLALADNVTVNIPNGSYEFSNNINGDAGLVKN
                     GQGELILSGASTFEGDTTLNEGAVTINGKKYVSKVNVSPSGTLVVNNTTLENGVNNEG
                     TVVNQGYSTIKRAYVASAQSTLVSDLGSNLNVKGEVDLNGSTLSLAAVKEGKAQYVTQ
                     KGLSMSAITSDSAIKGNFAAIETSGLLTATAEKTNENEVKATVSRKNVATALESSFSA
                     LDNQIEGNNGSTNATNDEFAQQAATLQNSIANINTQSVVLDSLSGQIYASAQALTFEN
                     SETVNKDLSNRLVMLGTLDNVGETAGV"
     gene            6330..7145
                     /locus_tag="PXA33_000780"
     CDS             6330..7145
                     /locus_tag="PXA33_000780"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDL5120134.1"
                     /translation="MYGNGTLKEIGFGEGKTNTYAGQIGFDKPVTDHVILGAALNYSK
                     AKVNFDRYGGSSKADGIGASLYARIGNKHKTPWYLQGRAGYGSVDSDVERHIILADNN
                     ASTAKINHRDRVFSAYVESGYDFKQGRFALTPFVGFSHDVVRRGAFSEQNSQFGLTAD
                     KATYKQTSGLIGLRTSLEVNLAGMKTTFQGYVNHQKAFNREDLSFKASYTGLQDAKFD
                     VKGIGLAKHKTWVGIGALAEVNKNTSLYVNYDLKLAKNSGHNNVVTAGIRINF"
     gene            complement(7232..8092)
                     /gene="pdxY"
                     /locus_tag="PXA33_000781"
     CDS             complement(7232..8092)
                     /gene="pdxY"
                     /locus_tag="PXA33_000781"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005721193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase PdxY"
                     /protein_id="HDL5120135.1"
                     /translation="MKNILSIQSHVVYGYAGNKASTFPMQLLGVDVWALNTVQFSNHT
                     QYGKWGGMVMPKEQIGSIVDGIHNIGELHRCDAVLSGYIGSAEQVAEIIQAFHKVKAE
                     NPNAIYVCDPVMGHPDKGCIVADGVKEGLINLAMAQADIITPNLVELRELTGLTVENF
                     EQAVEAVKVILTKGPKKVLVKHLSRVGKESNKFEMLFANQDGIWHISRPLYQFAKEPV
                     GVGDLTAGLFVANLLNGKSDLEAFEHTANAVNEVMEQTARSGLYELQIIASRDRIVNP
                     QCSYQAVKIA"
     gene            8211..8286
                     /locus_tag="PXA33_000782"
     tRNA            8211..8286
                     /locus_tag="PXA33_000782"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8244..8246,aa:Thr,seq:tgt)
     gene            complement(8381..9334)
                     /gene="accA"
                     /locus_tag="PXA33_000783"
     CDS             complement(8381..9334)
                     /gene="accA"
                     /locus_tag="PXA33_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HDL5120136.1"
                     /translation="MSQEFLDFELPIAELEAKIESLRAVSEQDDKINLDDEIKRLQKK
                     SEELTKKTFANLDAWQISRMARHPNRPYTLDYIEHIFTEFDELAGDRAFADDKAIVGG
                     IARLDGRPVMVIGHQKGRTVKEKVKRNFGMPAPEGYRKALRLMEMAERFNMPIITFID
                     TPGAYPGIGAEERGQSEAIARNLREMSQLSVPVICTVIGEGGSGGALAIGVGDKVNML
                     QYSTYSVISPEGCASILWKSADKASTAAEVMGLTAPRLKELELIDNIVPEPLGGAHRN
                     VEQIAQNLKQRIVEDLAGLDQLNKEDLLERRYQRLMNYGYV"
     gene            complement(9388..10812)
                     /locus_tag="PXA33_000784"
     CDS             complement(9388..10812)
                     /locus_tag="PXA33_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HDL5120137.1"
                     /translation="MKKQITRRQFLQGSALAAGFATVPTPLLAAERPQLNIPPLMDIG
                     RGKPVRLDLRPAQTQFDKGKLVDVWGVNGQYLAPTVRVKSGDFVKLTYLNNLPQKISM
                     NIQGLQASSEMIGTVHRSLDPKSSWSPIVSINQPACTCWYHADTMLNSAFQVYRGLAG
                     LWMIEDEQSKKATLPNKYGVNDIPLILQDQLLNKDGVQVLDKNTSQFFGKRLFVNGQE
                     SPYFNVPRGWVRLRIVNASLSRAYELRLDNEQPLYLIATGLGMLAEPLETESVQLAPS
                     ERVEVLVDLSEGKAVSLISGQKRDIFYKIGQVFADNDELNDNVILELRPEGMAAVLNN
                     KPSLPAFNLEEFNLKISQERRFAIRPADYLINQKRFDPKRIDFTAQKGSVERWYLHST
                     QAVGFTLQGAKFIIETLNRKQYPIKQLAWRDTVWLEAEQEITLLVRFDNTAPEQLPFT
                     FGVSDFMLRDRGTMGQFSVIENGQ"
     gene            complement(10814..11548)
                     /locus_tag="PXA33_000785"
     CDS             complement(10814..11548)
                     /locus_tag="PXA33_000785"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HDL5120138.1"
                     /translation="MLKIFRILIVALAAIFISIFGTIYALLRLRHPSSVGVVARWFGA
                     LHKLVGLELITRKKPEINYPVVYIGNHQNNYDMVTISSMVAEKTVSIGKKSLIWIPFF
                     GLVYWATGNIFIHREKRSSAIETMNKAGQIVKERNVSVWMFPEGTRSRGRGLLPFKTG
                     AFHLAISAGVPIVPVVCSNLHNKVDLNRSNNGTVICEMLDPIDTSGYSRENVKELMEK
                     CHQIMLAKFEQLNQEVEQLENTAKNA"
     gene            11695..12192
                     /gene="tpx"
                     /locus_tag="PXA33_000786"
     CDS             11695..12192
                     /gene="tpx"
                     /locus_tag="PXA33_000786"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005578656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HDL5120139.1"
                     /translation="MSKVTLAGSPIDVAGTFPQKGDVVADFTLVNSDLSDVSLSDFDG
                     KRKVLNIFPSIDTGICATSVRVFNQKAADVANTVVLCISADLPFAQARFCGAEGIENA
                     KVLSSFRHRDLHNKLGVDIISGPLAGLTSRSVIVLDENNKVLHSELVPEIKQEPDYEA
                     ALAVL"
     gene            complement(12277..13902)
                     /gene="yidC"
                     /locus_tag="PXA33_000787"
     CDS             complement(12277..13902)
                     /gene="yidC"
                     /locus_tag="PXA33_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDL5120140.1"
                     /translation="MNNRGLLLMGLFLVSILIFTQWQQDFDPEIQAQKQAQVAAQTQQ
                     AGDLPSASNANNPIADSITQGKTITVESDVLRLTIDTLGGDVIGSDLLKHNAELHSNT
                     PFKLLQQDGDKIYVAQSGLVGKNGIDTNAGRPQYAVTADSFKLAEGQNEISVPLTFEK
                     EGVVYTKTFTLKRGSYDVGVNFNIKNNTAQAIEVQPYGQLKHSIVESSGNLTMPTYTG
                     GAYSSSETNYKKYSFQDMEKANLSVDTKAGWIAVLQHYFVSAWVPNQDADNSLYSRTN
                     NGVATIGYRGPLTTIAPNSEANLNSKLWTGSKDQKEMGESANHLDLTVDYGWAWFIAK
                     PLFWLLTAIQKIVSNWGLAIIGVTIVVKTILYPLTKAQYTSMAKMRMLQPRITEMRER
                     FGDDRQRMSQEMMKLYREEKVNPMGGCLPILIQMPIFIALYWTFMEAVELRHAPFFGW
                     IQDLSAQDPYYILPLLMGGSMFLLQKMSPTPVADPVQQKVMTFMPVIFTVFFLWFPSG
                     LVLYWLTSNLITIAQQWLIYRNLEKKGLHSRKK"
     gene            complement(14055..14480)
                     /locus_tag="PXA33_000788"
     CDS             complement(14055..14480)
                     /locus_tag="PXA33_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HDL5120141.1"
                     /translation="MTIWKQTATCEQLNTLSQKSAVSHLGIEFTAIGEDWIEAQLQVD
                     ERTQQPFGVLHGGVSAALAETTANAGALMVCEPHQIAVGMELNISHLKSIPAGTKAIA
                     KATPLKLGREVQVWNIDIKDEQGNLCAVARLSTKTLDKR"
     gene            complement(14809..17901)
                     /locus_tag="PXA33_000789"
     CDS             complement(14809..17901)
                     /locus_tag="PXA33_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDL5120142.1"
                     /translation="MIPRLSEVPQLDKATQSYLTELVNQHFSGEIASNYADRLSLATD
                     NSVYQQIPQAILFPKSVSDVVILTKLAQKPEYQHLTFTPRGGGTGTNGQSLNNNIIVD
                     LSRHMNQILELNVEERWVRVQAGVVKDQLNQFLKPHGLFFSPELSTSNRATLGGMINT
                     DASGQGSLQYGKTSDHILAIKSVLINGDILETQAVKSADFFAHIEGLNLSALGKTLHR
                     EIFNRCKTLRPKVLNELPQLNRFITGYDLKNVFNHGESEFNLTRILTGSEGSLAFICE
                     AKLNLLPIPKYRTLINIKYNSFDAALRSAPFMLEAKALSVETVDSKVLNLAKQDIVWH
                     SVSDLLTEDPANPILGINIVEYASSDQTQIETQVKALCQSLDQKMANNEAGIIGYQLT
                     SDVNSIEKIYAMRKKAVGLLGNAKGWAKPIAFVEDTAVPPENLADYIVEFRALLDRYN
                     LPYGMFGHVDAGVLHVRPALDLCDKAQVETFKQISDQVVELTAKYGGLIWGEHGKGMR
                     SYYGERFFGETLWQELRYIKTLFDPYNRLNPGKICTPLESETSLYPIDSQMRADFDRQ
                     IPIQVREEFKGAMNCNGNGLCFNFDVHSTMCPSMKVSGNRLYSPKGRATLVREWLRLL
                     TEQNVSPDDLIFKPRKQAVKLADFVAKIRNTLNKNKEYDFSHEVKAAMDTCLACKACA
                     SQCPIKIDVPTFRSQFNELYHSRYLRPLKDYVVSNLEFIAPLMAKQPRFFNFFSTSTF
                     AEKAANKTIGMTYLPALSVPNLQHQLVEVGYQGESLESLEQQATSGDFSAKNCKTLFI
                     VQDPFTSYYDAKVVADFIALIKKLGFKPIVLPFKPSGKAQHVKGFLHKFAKTAKNQAD
                     FLNRVAKLGLPMVAVDPALTLLYREEYKEILKEARGDFKVLLAHEWLREVIKNGELEN
                     CKKSANSDRLPWYLFAHCTESTALPSSMKEWQAIFTHFGETLLNENVGCCGMAGTFGH
                     ETKHVEMSKAIYQQSWQIKLKNKPLERCLATGYSCRSQVKRFEQQGIKHPIQALLDVI
                     "
BASE COUNT         5126 a         3638 c         3935 g         5297 t
ORIGIN      
        1 atttttgtga ttgtttatga ttggttttga ttgcattgtt cttattttag cgtaaatcag
       61 gagactatta tgagcaaaat tcaagaaatc actcgtgaaa gctggatcct ttcaactttc
      121 cctgaatggg gtacttggtt gaatgaagaa attgaagaag aagttgtacc ggaaggcaat
      181 tttgcaatgt ggtggctagg ctgtgtcggt gtgtggatta aaaccccagc tggtgcgaat
      241 atctgtatgg atttatggtg cggtcgtggt aaatccacca agaaagtaaa agatatggtg
      301 cgtggtcatc aaatggcaaa tatggcaggg gtgcgtaagc tccagcctaa tttgcgggct
      361 caaccgatgg tgttagatcc gtttgcgatt aacgaagtgg acttcattct cgcttctcac
      421 tatcactcag accatattga tgtaaacgtg gcagcggcta tttgcaataa cccgaaatta
      481 gaccacgtta aatttgtcgg cccttggcac tgtgcagaat tatggaaaaa atggggcgtg
      541 ccggaagaac gtattattgt ggtaaaaccg ggcgatgtcg tgaaattaaa agatgtagaa
      601 attcacgccc tagattcatt tgaccgtacc tgtttagtga ccttaccggt agaaggtgca
      661 gaagaacgtg gcggagaact tgcaggctta tgcccatcag atgaagaaat gggacgcaaa
      721 gcggtgaact atgtgttcaa aaccccgggc ggtacgattt atcacggtgc agattctcac
      781 tactcaatcc aattcgctaa acacggtaag caatttgata ttgacgtagc gctgaataac
      841 tatggcgaaa acccggttgg tattgcggat aaaatgactt cagttgattt attacgtatg
      901 gcggagtgtt tacgctgtaa agtgattatt ccggttcacc atgatatttg gacaaacttt
      961 atggcaagta cgcaagaaat tattgattta tggcgtatgc gtaaagatcg cttacaatac
     1021 caattccacc catttatttg ggaagtgggc ggcaaatatg tttacccacg tgataaagac
     1081 ttaattgaat atcatcaccc tcgcggcttt gatgactgct tcgagcaaga gccaaatatt
     1141 caatttaaat ccatgctata ggtgagcagt cgccactctc actggagagt ggcgttttag
     1201 caagttcatt atggagcagt agtgattagt aatgggataa agtagtgaac gaaagttata
     1261 gacacaaaga gttattagcc ctgttggaag agcggaaagc actgtcaaca gcagatatta
     1321 tgcagctgtt aaatatttct ccggcaacag caagacgtga tatcagtaaa ttagatggtc
     1381 aaggtaaatt aaaaaaggtt agaaacggag cagaagcaat tctggctcct acatttttac
     1441 agcacacaaa aataactaac aatgcgcagg agaaacgtag aatagcggaa gctgccggtc
     1501 gtttatgtaa agatggggaa agtcttattc tgacgtgtgg ttccacaatg gaaatgctcg
     1561 gtgaagtatt atgtggtaga aaattgcaaa ttattaccaa ttatctaccg cttgcaaacc
     1621 agttgattgc aggttgccac gatgatgtag tgattatggg tgggcaatat aataaacata
     1681 aaaaagtgat gctctcaatt aacacccaaa atgagatgac ttatgccgct accacaatgt
     1741 ttaccagtgg caaaggctta accagcgaag gattatttaa aaccgatatg attattgcca
     1801 atgccgagca acaaatggca aagcacgcag aacgttatgt ggtgttattg gatagcacca
     1861 agctcggcaa aaaagtcggt atgctgttta aagagttcga taaaattgat ttattgatta
     1921 ccggcaaaga ggcggatccg actattatcc aagcattaag aaacaaaggc ctggatatta
     1981 tcttggctta gcctgtagcg gtcaaattta atcaaaattt tacttatcat cttcaaggga
     2041 aaactatgag aaaacataaa ctgggtattt acgaaaaggc cttgccgaaa aacatttcgt
     2101 ggcaagaccg cctttctatc gccaaagtct gcggttttga ctttgtggaa atgtcgattg
     2161 acgaaacgga cgaacgctta gcccgccttg attggagcaa aaaagaacgt gccaagctgg
     2221 tgaaaaccat catcaacacc ggcgtgacca ttccgtcaat gtgcttgtcc ggtcatcgcc
     2281 gtttcccgtt tggttcttgt gatgaagcga cccgccaaaa agcctatgaa attatggaaa
     2341 aagccattca actggcggtc gatctcggta ttcgcaccat tcagcttgcc ggctatgacg
     2401 tctattacga agagcaggac gaaggcacaa tccaacgctt ccaacaaggc ttagaatggg
     2461 cggtggagct ggcggcaagc aaccaagtta ccattgcggt ggaaattatg gacacccaat
     2521 ttatgagttc gatttcccgc tggaaaaaat gggacgagat catcaaatca ccgtggttca
     2581 ccgtgtatcc agatttaggc aacctctcag cgtggaacga caacgtagcg gaagagctga
     2641 aactcggcat cgacaaaatc tctgccattc acttaaaaga cacttacaaa gtgactgaaa
     2701 cctgcaaagg gcagttccgt gatgtgccat ttggtgacgg ttgcgtagat ttcgccgctt
     2761 gttttaaaac ccttgccgag ctgaattacc gtggcgcatt cttaattgaa atgtggacgg
     2821 aaaaagccga agagccgatt actgaaatca tcaacgcccg ccgttggatc gaacaaaaaa
     2881 tgcaagaagg aggatttacc ctatgacgca attaactgaa ttaacccctg cattacaagc
     2941 cttaagagag cgtgtgttaa aagccaattt agaattaccg aagtatggct tggtaacttt
     3001 cacttggggt aacgtaagtg aaatcgaccg tgagaccggt ttagtcgcca tcaaaccttc
     3061 cggcgtagaa tacgatgcga tgacggtgga agacattgta ttggttgatt tacaaggtaa
     3121 tcgagtttac ggagataaaa aaccgtcttc tgatacagaa acgcacttag aactttatcg
     3181 ccaattcact gatattggcg gtgtggtaca cactcactct cgcaatgctg taggttgggc
     3241 acaagcaggg cgtgatttat tggcacttgg caccactcaa ggcgattatt tctacggtgc
     3301 gattccttgt actcgcaaaa tgaccgcaga ggaaattgcc ggcagttatg agctggaaac
     3361 cggaaaggtg attgtcgaaa ctttccgaac tcgaggtatt gaaccgaaag cagttccagg
     3421 tgtgttggta cattcacacg gtccatttac ctggggaaaa gatggacacg atgctgtgca
     3481 taattctgtg gtattagaag aggtggctta catgaatttt attagccatc aaattcgccc
     3541 acaaattggc ccaatgcaac aggaattatt agataagcat tatctgcgta agcatggtgc
     3601 aaatgcttat tatgggcaat agctaataac ctaaaaatta ttttccaaat tatcaacagc
     3661 tttcgagggg ctttctataa tcggaatagc tcctcagtct gtttgagttt atggatagat
     3721 ccacaaataa ttaccatttt ccttttcttg attttaattt tgatttttgt tctaatctct
     3781 gcctcttgtt tttacaagtc tattaaggat ccgtagtaga tttgaaataa acaagagtat
     3841 ctttgtattt attagaagga aaccaaaagg tgaaaaatag aaattatttt gttgcaatag
     3901 caacaatatt agccttatcg ccagaggtta acgcaactga actttcagat cagcttgcaa
     3961 gtagaacaag atattatcct tccgccacgg atagttatta tagttactat aaatatgcac
     4021 cgatttattc gggctactat acatcgaata gccgtaatgg ttattcgtcg tggtattatc
     4081 caggtagcaa tgcaggtgta acaaccagaa ctgtttcgac aacagttaat ggcattacag
     4141 ttacaaaagt ttatacaacg cataatggga gaacaaccgt tcgtaccact tatgctcctg
     4201 ctgtgaacaa accaattgta aataaaccga caccgattgc caataaaccc gttgtagata
     4261 cgccaactca accggttatt gatgccaacc caattatgga tccgccagtc caaccaatag
     4321 ttgataataa acccatcact gatacacagc ctaatccaat tacagataac aaacctgtca
     4381 ttgaatcgcc aattgatgac acccgtccag ttatcattag gcctacaaca ccggtaaatc
     4441 ttcatcctga acctgtagct gttggtgtac ccaatgtagg tattcgatgg aatgaccgct
     4501 ctcgtggtta caacattgat gatcctaata acaaacaaaa tgttaagtta actggtgaag
     4561 gtgttaaagt cggtatctta gatagtggtt ttaaaaacta tttaatgcag gacgatatac
     4621 tcaaaaaatt tggtaatcgc gctgttatta ttgaaacagg gcgtactgcc ccggcttctg
     4681 caacacatgg tattgaggtc gcagaaatgg ttgggggagg gaactctcgc aatggagtag
     4741 cgtctaagtc aacgttatta cttgcggata ttaccaaaac aacaaatgcg ggtacaggat
     4801 tatccgcaac agctccaatt tattatgact tatggaatag gggagcccgt atttttaacc
     4861 aatcatacgg tattccaaag cctcttactt attttaataa caatagaaat tctagctatt
     4921 actatttaca ccaatttgat ggtaatgtgt tgcaattcta taaggataaa gtaaaagagg
     4981 ggggcttatt tgtctgggct gcggggaata accgtggtga tcttgatcca tcactccaag
     5041 ctgggttacc tcattatgaa gctgacttgg aaaaaggttg gatttctgtt gttgcggtag
     5101 caaccagaac taatcaggga ttgggtaact ttgaatggag caatttattg ccatattctc
     5161 aagccggtgt ggcaaaaaat tggaccgtta ccgcaatggg tgactacatc tttaatttag
     5221 gaggtagaaa cattattgct agtggttcat cctttgcagc tccagccgtt acgggtacag
     5281 cagctctcgt gaaacaaaaa tatccttgga tggatggtaa ccttatcaaa caatctattc
     5341 tctcgacggc aaccgatatc ggcgctcctg gagttgatga tgtatatgga tggggtttac
     5401 ttaatattga aaaagccact aatggccctg cgcgttttgg taaacgactc gctttagccg
     5461 ataacgtgac tgtaaatatt ccaaatggaa gctatgagtt ttccaataat attaacggtg
     5521 atgcaggctt ggtgaaaaat ggccaaggcg aattaatttt atcgggtgct agtacttttg
     5581 aaggggatac aacactcaat gaaggtgctg tcactattaa cggtaaaaaa tatgtgtcta
     5641 aagtaaacgt atctccaagt ggtacattgg ttgtcaataa taccacgtta gaaaatggcg
     5701 taaataacga gggtacagtt gtcaaccagg gttattctac gattaagaga gcttacgttg
     5761 cttcagcaca aagtacgcta gtgtcagatt taggttcaaa cttaaatgta aaaggagaag
     5821 ttgacttaaa cggctctact ttatcactag ctgcggtaaa agagggtaaa gctcagtatg
     5881 tgacccaaaa aggtttatca atgagtgcta ttacttctga ttccgcaatt aagggtaact
     5941 ttgctgcaat agaaacctca gggttattaa ctgcgacggc ggaaaaaacc aatgagaatg
     6001 aagtaaaagc aacagttagc cggaaaaatg tcgcgactgc tttagagagc tcattttcgg
     6061 cattggataa ccaaattgaa ggaaataatg gttctacaaa tgctacaaat gatgaattcg
     6121 cacaacaagc ggctacttta caaaacagta ttgcaaacat caatacgcaa agtgtggttt
     6181 tagatagctt atctggtcaa atttatgcgt ctgcgcaagc gcttactttt gaaaactcag
     6241 aaacagtgaa taaagatctc tctaaccgtc ttgtaatgtt aggaacgctg gataatgtag
     6301 gtgagactgc tggtgtgtga gtaacgggta tttatgggaa tggtactcta aaagaaatag
     6361 ggtttggtga aggtaaaacc aatacctatg ctggccaaat aggttttgat aagccagtta
     6421 cagaccatgt gattttaggt gcggcactta attattctaa agcaaaagtg aacttcgatc
     6481 gttatggcgg tagttcaaaa gcagatggaa taggtgcatc attatatgcc cgtataggca
     6541 ataagcataa aacaccttgg tatttacaag gtcgagccgg ttatggcagt gttgattcag
     6601 atgtagagcg tcatattatt ttggcggata ataacgcaag tacagcaaaa atcaaccacc
     6661 gagacagagt attttctgct tatgttgaat caggctatga cttcaagcaa ggtcgctttg
     6721 cgttaactcc atttgtcggt tttagtcatg atgttgtcag acgtggcgca ttcagtgagc
     6781 aaaacagtca atttggtctc accgcggaca aagcaaccta taaacaaaca agcggattga
     6841 ttggtttacg aacatcttta gaggtgaatt tagctgggat gaaaacgact ttccaaggct
     6901 atgtaaatca tcaaaaagcg tttaatcgag aagatcttag ctttaaagca agttatacag
     6961 gtttacaaga tgcaaaattc gacgtaaaag gtattgggct tgcaaaacat aaaacgtggg
     7021 tagggattgg tgcattggcg gaagtaaata aaaatacttc cttatatgtg aattacgacc
     7081 ttaaactcgc aaaaaatagt ggtcacaaca acgttgtgac tgctgggatt agaattaatt
     7141 tctaactgaa tctttttaaa caaaacgggc gtttatacgc ccgttttttt atagttatag
     7201 agataataat gttattttat acttcattta attaggcaat ctttacagct tggtaactac
     7261 actgtggatt aacaatccga tcacgagaag caataatttg taactcataa agccctgatc
     7321 tagcggtttg ttccattacc tcattgaccg catttgcagt gtgttcaaag gcttctagat
     7381 cggattttcc atttaataag ttcgccacaa atagccctgc ggttaaatcg cctacaccga
     7441 caggttcttt ggcaaattgg tagagtgggc ggctgatgtg ccaaatgcca tcttggttgg
     7501 cgaacagcat ttcaaatttg ttgctctctt tgccgactcg gcttaaatgt ttaaccaata
     7561 cttttttcgg gcctttggtt aaaatcactt tcactgcctc gaccgcttgc tcaaaatttt
     7621 ccaccgttaa accggttaat tctcttaatt ccactaaatt tggtgtgata atgtcggctt
     7681 gtgccattgc cagattgatc aatccctctt ttacgccgtc tgccacaatg cagcctttgt
     7741 cggggtgtcc cattaccggg tcgcatacat aaattgcgtt tggattttcc gctttcactt
     7801 tatgaaaagc ctgaataatt tcggctactt gttctgccga gccgatatag ccagagagta
     7861 cggcatcgca acgatgcagt tcaccgatat tgtgaatgcc atcaacaatg ctaccgattt
     7921 gttcttttgg catcaccata ccgccccatt ttccatattg ggtatggttg gaaaactgta
     7981 cggtatttaa cgcccataca tcaacaccta acagctgcat aggaaaagtt gaagccttat
     8041 tgccggcata gccgtagaca acgtgcgatt gtatggatag tatgtttttc attcattcac
     8101 tcctttaaaa tttcaatatg tgttgttttt tagtcatttg aagttgcaag gacttgaagc
     8161 tcatcaagaa atctctataa tcccgcctcg ttctcttatc cattcaatat gccgacttag
     8221 ctcagtaggt agagcaactg acttgtaatc agtaggtcac cagttcgatt ccggtagtcg
     8281 gcaccatcac ctttattttc tgatgagtcc cctttaatgt aaaatggcaa gattttgcaa
     8341 attttcacaa aaatcttacc gcttgtaggg tgaaattgaa ttagacgtaa ccgtaattca
     8401 ttaagcgttg ataacggcgt tccagtaaat cctctttatt aagttgatct aaccctgcca
     8461 aatcttcaac aatacgctgt ttaaggtttt gggcgatttg ttcaacattg cgatgagcac
     8521 cgcctaaggg ttccggcacg atgttatcaa tcagctcaag ctcttttaaa cgaggggcag
     8581 ttaaacccat tacttctgct gccgttgagg ctttatcggc acttttccac agaatcgagg
     8641 cacagccttc aggtgaaatt accgagtaag ttgaatattg caacatattg accttatcgc
     8701 ccacaccgat cgctaatgca ccgcccgagc caccttcgcc aattaccgta cagattactg
     8761 gcacggaaag ttgagacatt tcacgcaggt tacgggcaat cgcttctgac tgaccacgct
     8821 cttccgcacc aatgcccgga taagctcccg gtgtgtcgat aaaggtgatg attggcatat
     8881 taaagcgttc tgccatttcc attaaacgta acgctttgcg atagccttca ggagcaggca
     8941 taccaaagtt acgtttcact ttctctttta cggtacgacc tttctgatga ccgataacca
     9001 tcaccggacg tccgtccaga cgggcaatac caccgacaat cgctttatca tcggcaaagg
     9061 cacggtcgcc tgccagttcg tcaaattcgg tgaaaatatg ctcaatataa tctaaagtgt
     9121 acgggcgatt ggggtggcgt gccatacggg aaatttgcca cgcatctaaa ttagcaaagg
     9181 ttttcttggt taattcctcg cttttctttt gtaaacgctt gatttcatca tcaagattaa
     9241 ttttgtcatc ttgctcgctg actgctctta aagactcgat cttggcttcc aattcagcaa
     9301 tcggcaattc aaaatctaaa aattcttggc tcattctatt ttccttttta tgggcaaaat
     9361 tatggggcat tatcgcttat cagtcggtta ttgtccattt tcaattacgc taaattgacc
     9421 catcgtacct ctatcacgca gcataaaatc gctcacgcca aaggtaaatg gcagttgctc
     9481 aggggcagta ttatcaaagc gaaccaacag cgtgatttct tgctccgcct cgagccaaac
     9541 ggtgtcccgc cacgcaagtt gtttgatagg gtattgcttg cggtttaggg tttcaataat
     9601 gaattttgca ccttgtagcg taaatccgac cgcttgtgtg ctatgtaaat accaacgctc
     9661 aacgctgcct ttttgcgcgg taaaatcaat gcgttttggg tcaaaccgct tttggttgat
     9721 taaataatca gccggacgaa tggcaaaacg tctctcttgg ctgattttca ggttaaattc
     9781 ctctaagttg aaagccggta aactcggttt attattcaat accgctgcca ttccttcagg
     9841 gcgaagctct aaaatcacgt tatcattcaa ttcgtcgttg tcagcaaaga cctgcccgat
     9901 tttgtaaaaa atatctcttt tttgaccgct tatcagcgat accgctttcc cttcgcttaa
     9961 atcgaccaac acttccaccc tttcactcgg tgcaagttga accgattcgg tttcaagtgg
    10021 ctctgccagc attcctaagc cggtggcgat taggtacaac ggctgctcgt tatctaagcg
    10081 taattcataa gcacgggaaa gtgaggcatt gacgatccgc aatctgaccc agccacgagg
    10141 cacgttaaaa taaggcgatt cctgcccgtt gacaaataat cgcttaccga aaaattgtga
    10201 ggtgttttta tctaacacct gtacgccgtc tttgttgagc agctggtctt gcagaattaa
    10261 cggaatgtcg ttcacgccat atttattcgg taaagtggct tttttgctct gttcatcttc
    10321 aatcatccac aagccggcta agccacgata cacttggaat gccgagttga gcattgtatc
    10381 ggcgtgatac caacaagtac aagccggttg attgatagaa acaatcggcg accagctact
    10441 tttggggtca agggaacgat gcaccgtgcc aatcatctca cttgaggctt gcaatccttg
    10501 aatattcatt gagatttttt gcggtaaatt attgagataa gttaatttga caaaatcccc
    10561 cgattttacc cgtacggttg gggcaagata ttgcccgttc acaccccata catcaaccaa
    10621 tttgccttta tcaaattggg tttgtgcggg gcgtaaatcc aaccgaaccg gctttcctcg
    10681 cccaatatcc attaaaggcg gaatgttcag ttgcggtcgc tctgctgcta atagcggtgt
    10741 tggcactgtt gcaaatccag ccgcaagggc agagccttgt aaaaattggc gtcgggtgat
    10801 ctgttttttc atattaggcg ttttttgcag tgttttctaa ctgttcgact tcttgattaa
    10861 gttgttcaaa ttttgccaac ataatttgat ggcacttttc catcagttct ttgacgtttt
    10921 cacgggaata gcctgaagta tcaatcggat caagcatttc acagattact gtgccgttat
    10981 ttgaacggtt caaatccact ttattgtgta aattagagca aactaccggc acaatcggca
    11041 cgccggcaga aatggctaaa tggaatgcac cggttttaaa tggcagtaaa ccacgcccac
    11101 ggctacgagt accttccggg aacatccaca ccgacacatt gcgttctttc acgatttgcc
    11161 ctgctttatt cattgtttca atcgcactgg aacgcttttc acggtgaata aaaatattgc
    11221 cggttgccca gtaaaccaaa ccgaaaaacg gaatccaaat caggcttttt ttgccgatac
    11281 tcaccgtctt ttctgccacc attgaggaaa tcgttaccat atcgtaattg ttttgatgat
    11341 tgccgatata gacaaccgga tagttaattt ccggcttttt gcgggtaatt aattccaacc
    11401 cgactaattt atgcaaagca ccaaaccaac gagctaccac acccacgcta cttgggtgac
    11461 gtaaacgcag taacgcataa atagtgccaa aaatagaaat gaaaatagcg gcaagggcaa
    11521 caattaaaat acgaaaaatt tttaacataa tggaataacc ttaaaaataa attgtctagg
    11581 agtatagcaa aataaggtta ttttctaaag gctatccaca ttaaattgat agtttcctat
    11641 tttgcaaaaa atcggtatat ttaaaccgct tgtttatttg ataaggagaa aaaaatgagt
    11701 aaagtgactt tagcaggaag cccgattgat gttgcaggca catttccaca aaaaggcgat
    11761 gtggttgccg attttacctt agtcaacagt gatttaagtg atgtttcgct ttccgatttt
    11821 gatggcaaac gcaaagtgtt aaatattttc ccaagtattg acaccggcat ttgtgccact
    11881 tctgtgcgtg tgtttaatca aaaagcggct gatgtagcga atacggttgt cctttgtatt
    11941 tcagcggatt taccttttgc ccaagcacgt ttctgtggag cagaaggaat tgaaaatgcc
    12001 aaagtattat ccagtttccg tcatcgtgat ttacacaaca aactgggggt agatattatt
    12061 tcaggtccgt tggcaggctt aacttcacgt tcagtaattg tgttagatga aaacaacaag
    12121 gtattacaca gcgaattagt gccggaaatt aaacaagagc cggattatga ggcggcgtta
    12181 gcggtattat aatttgcaga attatgctgg aaaataaccg cttgtaaagc aacaaaaagc
    12241 ccgttgattc aattcaacgg gctttgtttt tagtgattat ttcttacgag agtgtaagcc
    12301 ttttttctca aggttacggt aaatcaacca ttgctgtgca atcgtgatta agtttgaggt
    12361 taaccagtaa agcactaagc ctgatgggaa ccataagaag aacacggtaa agataaccgg
    12421 cataaaggtc attacttttt gttgtaccgg gtcagctacc ggtgttggcg acattttttg
    12481 taacaagaac atcgaaccac ccattaataa aggcaaaatg tagtatgggt cttgtgccga
    12541 taagtcttga atccagccga agaaaggtgc gtggcgtaat tctaccgctt ccataaatgt
    12601 ccaatataaa gcaatgaaaa tcggcatttg gattaaaatt ggtaagcagc cacccattgg
    12661 gttcactttt tcttctctgt agagtttcat catctcttgg ctcatacgtt ggcggtcgtc
    12721 gccgaaacgt tcacgcattt cagtaatgcg aggttgtaac atacgcattt ttgccatcga
    12781 ggtgtattgt gctttggtta atgggtaaag aatggttttc actacaatag tgacaccgat
    12841 aattgccaag ccccagttgc ttacgatttt ttgaattgcg gttagtaacc agaaaagcgg
    12901 tttagcgata aaccacgccc agccgtaatc tacggttaag tctaggtgat ttgctgattc
    12961 gcccatttct ttctgatctt tagaacctgt ccaaagtttg ctatttaaat tagcctctga
    13021 atttggcgca atcgtagtta aagggccacg gtaaccgata gttgctaccc cattattggt
    13081 gcgtgaataa aggctgttat ccgcatcttg gtttggcacc caagcagaca cgaaatagtg
    13141 ctgtaatacc gcaatccaac ccgctttggt gtccacgctt aagttggctt tttccatatc
    13201 ttggaaactg tattttttgt agttggtttc tgaagaagaa taagccccgc cggtgtaagt
    13261 cggcatggtt aaattgccgg aactttcaac tatagagtgt tttaattgcc cgtaaggctg
    13321 aacttcaatc gcttgtgcag tattattttt gatattgaag tttaccccaa catcgtagct
    13381 accacgtttt agcgtgaaag ttttggtgta aaccacaccc tctttttcaa aggttaatgg
    13441 tacagaaatt tcattttgcc cttcggctaa tttgaagcta tctgccgtta ccgcatattg
    13501 tgggcgaccg gcattggtat caatcccgtt tttacccact aaaccgcttt gagcaacgta
    13561 gattttgtcg ccgtcttgct gtaatagttt aaatggcgtg ttagagtgta attcagcatt
    13621 gtgttttaac aaatctgagc cgatgacatc accgcctaag gtatcaatag tcagacgtaa
    13681 tacatcgctt tccacggtaa tggttttacc ttgcgtgatg ctatctgcga ttggattatt
    13741 ggcattggaa gcagacggaa gatcacccgc ttgttgggtt tgtgctgcca cttgagcttg
    13801 tttttgtgct tgaatttcag gatcaaaatc ttgttgccat tgtgtaaaaa tcaaaataga
    13861 cacaagaaat aaacccatta gcagtaagcc acgattattc atgttggaat tctcttgtta
    13921 gtaatggata aaaagaaagg tattttataa taggttagct tgttttccaa acgcctttta
    13981 tgaacaagcg gtcggatttt ccaagttttt tgcaaaattt tcggagaatt tgaccgcttg
    14041 tggagaataa gaaactagcg tttatctaag gttttggtgg aaaggcgagc aactgcacac
    14101 aggttacctt gttcgtcttt aatgtcgata ttccaaacct gtacttcacg ccctaatttc
    14161 agcggtgtag ctttggcaat cgcttttgtg cctgccggaa tcgattttaa atggctgatg
    14221 tttagctcca ttcccaccgc aatttgatgg ggttcacaca ccattaacgc ccctgcattg
    14281 gcagtagttt ccgcgagtgc tgccgataca ccgccgtgca atacgccaaa cggctgttga
    14341 gtgcgttcgt ccacctgaag ctgtgcttca atccaatctt caccgatagc ggtaaattca
    14401 atccctaagt gtgacaccgc cgatttttgg ctgagtgtgt ttaattgttc acaagttgct
    14461 gtttgcttcc aaatggtcat tattatttcc tgtctaataa aaaagctgtc tatttagaca
    14521 gctttcatta tctctttgag cgaaacatac tacgccaata gtacattgtt tggtcgccca
    14581 tattacctta agccatatag cactaaggag gtgatttatg tgagaaaatc agaagttttc
    14641 tttctggttg cgttgctaat attattagca acttccgcta gcattaaagt actcaccata
    14701 cttgatgtgc tagtttggtt attcgctaga taacctaaaa tcagacagag gcaggctggt
    14761 tacctgcctc gctttttatt ctaagtactt ttaggttaca tttcaacctt aaatcacgtc
    14821 cagcaacgct tgaattgggt gtttaatgcc ttgttgttca aaacgtttga cttgcgagcg
    14881 gcaggagtag ccggttgcta ggcaacgctc aagcggttta ttttttaatt taatttgcca
    14941 tgattgctga taaatcgctt tcgacatttc aacgtgtttg gtttcgtgtc cgaacgtgcc
    15001 tgccattcca cagcaaccga cattttcatt gagtaaggtt tcaccaaaat gggtgaaaat
    15061 agcttgccac tctttcattg agcttggcaa ggcggtggat tcggtgcagt gagcaaataa
    15121 ataccacggc aagcggtcgg aatttgccga ttttttgcaa ttttccagct cgccgttttt
    15181 aatcacttcc cttaaccatt cgtgtgccaa taagactttg aaatcccccc gagcttcttt
    15241 gaggatctct ttgtattctt cacgataaag caaagttaag gcaggatcga cagccaccat
    15301 cggtagcccc aatttcgcca cacggtttaa aaaatcggct tggtttttcg ctgttttcgc
    15361 aaatttatgc aaaaagcctt taacgtgttg tgctttaccg cttggcttga atggcaacac
    15421 aatcggctta aagcctaatt ttttaatgag agcgataaag tctgccacca ctttggcatc
    15481 gtaataagag gtaaacggat cttgcacaat aaacaaggtt ttgcaatttt ttgcagaaaa
    15541 atcaccgctt gtagcttgtt gttcaaggct ttctaagctc tcgccttggt agccgacttc
    15601 caccaactga tgttgcagat ttggcactga caatgccggc aaataggtca ttccaatggt
    15661 tttgtttgca gctttttcgg caaaagttga ggtgctgaaa aagttaaaaa aacgtggttg
    15721 tttcgccatt aaaggggcga taaattctaa gtttgagacc acataatctt tcagtggtcg
    15781 caaatagcgg ctgtggtaaa gctcgttaaa ttgcgatctg aacgtcggta cgtcaatttt
    15841 gattgggcat tggctggcac aggctttgca ggcaaggcag gtgtccatcg ccgctttgac
    15901 ttcgtgtgaa aaatcatact ctttgttttt attgagcgtg ttgcgaattt ttgcaacaaa
    15961 atcagctaat ttgaccgctt gtttgcgagg tttgaagatc aaatcgtccg gcgaaacgtt
    16021 ttgttcggta agtaatctga gccattcacg cacaagagta gcccgccctt tcggtgagta
    16081 aagacggttg cccgagactt tcattgaagg gcacatcgtg ctgtgtacat caaagttaaa
    16141 acatagcccg ttgccgttac aattcattgc tcctttaaat tcttctcgca cttggattgg
    16201 aatttggcgg tcaaaatcgg ctcgcatttg tgagtcaatc gggtagagcg aggtttcgct
    16261 ttctaatggt gtacaaattt tacctgggtt caagcgattg tacgggtcaa ataaggtttt
    16321 gatgtaacgg agttcttgcc acaaagtttc gccaaagaaa cgttcgccgt agtaagaacg
    16381 catacctttg ccgtgttcgc cccaaatcaa gccaccgtat ttggcggtga gttcgactac
    16441 ttggtcggaa atttgcttaa aggtttccac ttgggctttg tcgcataagt caagagcagg
    16501 gcgaacgtgt agcacgccgg catcaacgtg gccgaacatt ccgtaaggca gattatagcg
    16561 atctaataaa gctcgaaatt ccacgatgta atcggcaagg ttttccggtg gcacagcggt
    16621 gtcttctaca aaggcgatcg gttttgccca gcctttggca ttgcctagca ggcctacggc
    16681 ttttttccgc attgcgtaga ttttctcgat agaatttaca tcactcgtga gttggtagcc
    16741 gataatcccg gcttcattat ttgccatttt ttgatcaagg ctttggcaga gtgctttcac
    16801 ttgggtttca atttgagtct gatcgcttga ggcatattca acaatgttaa tccctaaaat
    16861 cgggttggct gggtcttcgg ttaataaatc tgacaccgaa tgccagacga tatcttgctt
    16921 ggcaagattg agcactttgg aatcaaccgt ttccaccgat aaggcttttg cctctaacat
    16981 aaaaggagcg gagcgtaagg cggcatcaaa ggaattatat ttaatattaa ttaaggtgcg
    17041 atatttggga atcggcagta aattcagttt ggcttcgcaa ataaaggcaa gcgaaccttc
    17101 agaaccggtt aaaatacggg taaggttaaa ttcgctttca ccatggttaa acacattttt
    17161 taaatcatag ccggtaataa aacggttaag ctgaggcagt tcgttcagca ctttggggcg
    17221 taatgttttg caacggttga aaatttcacg gtgcaaggtt ttgccgagag cggagagatt
    17281 taacccttca atatgtgcaa aaaaatcggc agatttgacc gcttgtgtct ctaaaatatc
    17341 gccgttaata agcacagatt tgatggcaag aatatgatca gatgttttgc catattgcaa
    17401 agaaccttgc ccagaggcat cggtgttgat cattccgcct aaagtcgctc ggttactggt
    17461 agaaagttcg ggggaaaaga aaagtccatg tggttttaaa aattgattga gctggtcttt
    17521 caccacgcca gcctgcactc gaacccaacg ctcttccaca ttcagttcca aaatctgatt
    17581 catatggcgg gagagatcaa caataatatt attgttaaga gattgcccgt tagtgcctgt
    17641 gccaccgcct cgtggagtaa aagttaaatg ttgatattct ggcttttggg caagtttggt
    17701 tagaattacg acatcagaaa cggatttcgg aaacagaata gcttgcggaa tttgctgata
    17761 cacgctgttg tctgttgcca gtgaaagcct atctgcataa tttgatgcaa tttccccact
    17821 aaaatgctga ttgacgagtt cagttaaata ggattgggtg gctttatcca gttgcggtac
    17881 ttcagagagt ctaggtatca taagctgtgt ttggtttttt ataataaaag cactaaattt
    17941 tgcttatctt agcaattttt ctaaaaattg ggaatggaat atcaatttga ttaaat
//
LOCUS       DANLRK010000032        17942 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.032, whole genome shotgun sequence.
ACCESSION   DANLRK010000032 DANLRK010000000
VERSION     DANLRK010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 17942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17942
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.032"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            <1..245
                     /locus_tag="PXA33_000790"
     CDS             <1..245
                     /locus_tag="PXA33_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HDL5120143.1"
                     /translation="IVAVTIGANNDQLITFNYIFAQSELRLSTLVAILFGLGLILGWL
                     VTGIFYLKIKFQNVALNRRVKRQAQQITELTTPKAE"
     gene            249..1445
                     /gene="lapB"
                     /locus_tag="PXA33_000791"
     CDS             249..1445
                     /gene="lapB"
                     /locus_tag="PXA33_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HDL5120144.1"
                     /translation="MLELLFLLLPIAALYGWYMGQRSAKKDQETASNKFSREYVTGLN
                     FLLSNQQEKAVDLFLSMLQKQESENHIASESQFEAELTLGNLFRSRGEVDRALRIHQT
                     LVNSPHYSFDQKLLAKQQLAKDFMVAGFYDRAENYYISLVDEPEFAKYSLSQLAVIYQ
                     KTREWKKAINVTEKRLRIEPDADKIPLSHFYCEYAQGVKFDDKAAFIESLNKALEYYP
                     QSTRASIILGDFYLEEGEYLRALSYFEQILVQDPDYISEVLGRIKQSYMALEDLNGYE
                     LFLIRANRVKHNSSVDIALTEFIEEKDGINAAHSKLYQQLSTYPNLITFHRFIRYQAD
                     FAEEGNGKESLVLLHNMVGNQIKRSFQYRCLNCGYQSYRLMWQCPSCNQWEKIKPVQS
                     IEGIIQ"
     gene            1469..2161
                     /gene="pyrF"
                     /locus_tag="PXA33_000792"
     CDS             1469..2161
                     /gene="pyrF"
                     /locus_tag="PXA33_000792"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HDL5120145.1"
                     /translation="MNNKVIVALDYETEREALEFVDLVDPSLCRLKVGKEMFTTLGTN
                     FVKQLHERKFDVFLDLKYHDIPNTVARAVRSAADLGVWMVDLHASGGLAMMEEAKKIL
                     EPYGKEAPLLIAVTVLTSMEDLDLLQIGINASPMEQVIRLAHLAQRAGLDGVVCSPQE
                     VEVLRTHCGQDFKLVTPGIRPKGTDFGDQRRVMTPKQAVETGSDFLVIGRPITQAQDP
                     LAVLKSINQSIS"
     gene            2244..2555
                     /gene="yciH"
                     /locus_tag="PXA33_000793"
     CDS             2244..2555
                     /gene="yciH"
                     /locus_tag="PXA33_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HDL5120146.1"
                     /translation="MTLVYSTETGRIKSEVIKEERPKGDGIVRIQRQTSGRKGKGVCL
                     ITGLDLEDKALNSLASELKRKLGCGGSFKDGVIEIQGDNRGFIKQILEQKGFKVKLAG
                     G"
     gene            2658..4820
                     /locus_tag="PXA33_000794"
     CDS             2658..4820
                     /locus_tag="PXA33_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HDL5120147.1"
                     /translation="MWKKTLIAVFLASQYAFAVGTGKEPSKSYTETELKQLKNDWLAI
                     YQKELQYAENLRLKQRENFQQINYLADAAISQKNLSLSTEQIIQRLLLTLAGYPLEMK
                     AEWKLLNAKITLKQATESEVKAFISKYPDSLYQRQLQNLMFELLYREQKFPELLEYAQ
                     NVKQSSLADQCRLFSARYELAASKAQINPEVSQVTEQKAENAELLALVQEFDRFWLAT
                     PKLTSDCANLESYWRDQGLKTDEKMRLKAVELIKQNANAELANLAANVNNENLKIWLS
                     SVEKVANNPADLQNFIENQPLDSPFYADNKAIIQQLFAKYVRTLSENMENPSFQQYQT
                     WAEKYQLSSEEINGWKNAFITRFFDNPEPTFQFWRDEQIKLLKTDNLTERRLRMAIWQ
                     KADLNEWLVLLSQQAKEKAEWRYWAAKEEKNEAKRKVDFESLAKERGFYAMLAAQQLG
                     IVYQIPMLEVPELTQAQLELYANQLARIQELRELGQFDQARKIWIDWIKNIKPEAGEN
                     TPTKIQLALIKYAKDKNWYDLAVEGTIQTKAFDYLDLRLPNAYSDWFDLHLQDKKISK
                     TFAQAIARQESAWNAQAQSHANARGLMQLLPTTAQKTAKDNGLTFKDGNDLFQPFNNI
                     MLGTTHLMELNEKYPNNRILIASAYNAGAGRVEQWLKRSNGQLAMDEFIASIPFYETR
                     GYVQNVLAYDYYYQMLYSDINDKNGLKMFYQEELTRKY"
     gene            4869..4945
                     /locus_tag="PXA33_000795"
     tRNA            4869..4945
                     /locus_tag="PXA33_000795"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4903..4905,aa:Met,seq:cat)
     gene            5172..5633
                     /gene="rimP"
                     /locus_tag="PXA33_000796"
     CDS             5172..5633
                     /gene="rimP"
                     /locus_tag="PXA33_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HDL5120148.1"
                     /translation="MATLEQKLEDLVLDSIEALGFELVGIECQRAGRFLTVRLYIDKE
                     GGVTIDDCSDVSRQVSAIFDVEDPIADKYNLEVSSPGLDRPLFTVAHYERFIGQDVVI
                     HLRIPMFDRRKWQGKIKQVEGDFITLTVENEDRQFAFGNIQKANLVPVFNF"
     gene            5654..7150
                     /gene="nusA"
                     /locus_tag="PXA33_000797"
     CDS             5654..7150
                     /gene="nusA"
                     /locus_tag="PXA33_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HDL5120149.1"
                     /translation="MSKEILLAAEAVSNEKQLPKEAIFEALETALTIATKKSRDLDID
                     VRVVVDRKTGDFQTFRRWAVVENVNNMTREISLEAAQFEDPNVQLGDFIEDEVESIEF
                     GRVAIQTFGQVVKTKIREAEKTKIIDEFRSDLGKIILATVKKVTRDNIFLELQGKSED
                     IKGEAVIVREDMIPRENFRPGDRVRGVLYEIKPESKGPQLFVTRAKPVMLEELFKLEV
                     PEIGEGVIEIKGSARDVGSRAKIAVKTHDKRIDPVGACVGMRGARVQAISNELGGERV
                     DIVLWDENPAQFVINAMAPADVSSIVINDETHSMDIAVEAKNLAQAIGRNGQNVRLAT
                     QLTGWALNVMTVEELTKKSEEEDGKVINLLTSALEIDEEFAQVLIEEGFTSLEAIAYV
                     PVDTLTAIDGLEDEDLVEELQSRAKNAITKKALEEEKLLKSAQIEEKLLTLEGMDRHI
                     AFKLAEKQITTLEELAEQGVDDLADIEELSEEKAGELIMAARQICWFN"
     gene            7135..9702
                     /gene="infB"
                     /locus_tag="PXA33_000798"
     CDS             7135..9702
                     /gene="infB"
                     /locus_tag="PXA33_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HDL5120150.1"
                     /translation="MLVQLSKEDNKMTEEKKTLSLGGARKASKVNTTTTSGKVKAVEV
                     KEKKPKIDAKALQEKAAKEAAAAKARAEKEAAEKLAKEKAEAEAKEKAAKKAAESTAS
                     KPISVPVMPSSKAKEEKPKAQQVQPKQEKAVDPEKEAKRKEEAELRRKQEELAQQKAE
                     MEAKRAAENARRLAEIAREEAEDNGNEDFDDERFTSSYAREAERDNDRRSEGNRGRNG
                     KGNVAKAKKGGREEDKNERSADRRNHKEVKGKGKQGKKGAALQQAFTKPAQVVKADVV
                     IGETITVAELANKMAVKATEIIKMMMKMGEMVTINQVIDQETAQLVAEELGHKVILRK
                     ENELEEAVLEDRDTHAEKVTRAPVVTIMGHVDHGKTSLLDYIRKAKVAAGEAGGITQH
                     IGAYHVETDDGKMITFLDTPGHAAFTSMRARGAKATDIVVLVVAADDGVMPQTIEAIQ
                     HAKAAGAPLVVAVNKIDKPEANPDRVEQELLQHEVISEKFGGDVQFVPVSAKKGMGID
                     DLLEAIILQSEVLELTAVKDGMASGVVIESYLDKGRGPVATILVQSGTLNKGDIVLCG
                     FEYGRVRAMRDENGKDVESAGPSIPVEVLGLSGVPAAGDEATVVRDEKKAREVALYRQ
                     GKFREVKLARQQKAKLENMFSNMAAGDVAELNIIVKADVQGSVEAISQSLMELSTDEV
                     KVKIVGSGVGGITETDATLAAASNAIMVGFNVRADASARRVIENENIDLRYYSIIYEL
                     LNDVKAAMSGMLQPEFKQEIIGLAEVRDVFRHPKFGAIAGCMVTEGIVKRNNPIRVLR
                     DNVVIFEGELESLRRFKDDVSEVRNGMECGIGVKNYNDVKVGDQIEVFEVVEVKRTI"
     gene            9787..10167
                     /gene="rbfA"
                     /locus_tag="PXA33_000799"
     CDS             9787..10167
                     /gene="rbfA"
                     /locus_tag="PXA33_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HDL5120151.1"
                     /translation="MSREFKRSDRVAQELQKEVAVILQREVKDPRIGMVTVSDVEVSR
                     DLAYAKIFVTFLFDNDAEAIKQGMQGLEKASPYIRTLLGKAMRLRIVPELRFIYDESL
                     VEGMRMSNLVSSVIKNDEEKHKED"
     gene            10170..11093
                     /gene="truB"
                     /locus_tag="PXA33_000800"
     CDS             10170..11093
                     /gene="truB"
                     /locus_tag="PXA33_000800"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HDL5120152.1"
                     /translation="MSRPRKRGRDIHGVFLLDKPQGMSSNDILQKVKRIFQANKAGHT
                     GALDPLATGMLPICLGEATKFSQFLLDSDKRYLVTAKLGERTDTSDAEGQVVETKAVA
                     VTKSQILAALEKFRGDILQVPTMFSALKHNGKPLYEYARAGITVEREARPITIFELNF
                     IEYKAPFLTLEVHCSKGTYIRTLVDDLGEELGCGAHVTMLRRTAVADYPIEKMMSYAD
                     LQNLAENQPLAELDQHLLPMDTAVSALAKINLSSEQTKAVGFGQRIRFDNLNQLYGLV
                     RLYSEDEQFLGVAEITNDTVIRPNRMINLTE"
     gene            11096..12176
                     /locus_tag="PXA33_000801"
                     /pseudo
     CDS             11096..12176
                     /locus_tag="PXA33_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252783.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
     gene            complement(12214..12738)
                     /gene="tadA"
                     /locus_tag="PXA33_000802"
     CDS             complement(12214..12738)
                     /gene="tadA"
                     /locus_tag="PXA33_000802"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HDL5120153.1"
                     /translation="MFIKPNQTSCPLQISEQDIAFMQYALDLADLAEAKGEIPVGAVL
                     VDKNQQIIGRGWNQTIQLCDPSAHAEMQAIRQAGQTLGNYRLLDCTLYVTLEPCPMCA
                     GAILHSRIKRLVFGASDYKTGAVGSRYHLFEDYKMNHFLEVHGNVLGRECSQKISDFF
                     KQRRLQKKGDALIK"
     gene            complement(12820..14595)
                     /locus_tag="PXA33_000803"
     CDS             complement(12820..14595)
                     /locus_tag="PXA33_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDL5120154.1"
                     /translation="MTTESLTFADLGLPQPILDAVNEMGFVNPSPIQQECIPHLLAGR
                     DVLGMAQTGSGKTAAFSLPLLAQIDPTQRHPQMLVMAPTRELAIQVADACEQFAKNMK
                     GIRVVTVYGGQRYDIQLRALKQGSQVVVGTPGRILDHIRRGTLDLSELKFIVLDEADE
                     MLRMGFIDDVETVMAELPETHQTALFSATMPEPIRRITRRFMKDPQEVKIKATERSAP
                     DIEQSYWLVNGFRKNDALLRFLEVEEFDAAIIFTRTKTGTIDITELCERNGYRTAALN
                     GDMTQQAREQTLDKLRSGRLDILVATDVAARGIDIERISLVINYDIPLEEESYVHRIG
                     RTGRAGRSGRALLFVEPRERRLLRNIENLIKKPINEVAIPNHEILMEKRREKFKARIS
                     KQLEHHDLEKYRELLEDLFTADQDHEELAAAMMMLLQEKQKLILPPDPVVRAARSERG
                     RDRNERNDRRNGREHRENNGVAMDLYRIELGREDGVEVRHIVGAIANEGDISSRYIGH
                     IKLYDKYSTIELPQGMPNHLVQHFAHKARVLSKPMQMTLLGSANSASNANPFEGRGRR
                     DGGFNKDRKGGFKEKRFSEKRGRRD"
     gene            complement(14797..15720)
                     /gene="nlpI"
                     /locus_tag="PXA33_000804"
     CDS             complement(14797..15720)
                     /gene="nlpI"
                     /locus_tag="PXA33_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HDL5120155.1"
                     /translation="MVQLFKLFRIRFLLLNLISVLMLSGCTSRYADLVSIKNLALAEL
                     NPQLRFEQEAMVVRLSQVLEEAPLTPSEQADLYFERGVIYDSLGLWSLARYDFAQAIS
                     LNQRMAAAYNYMGLYLLLEGDYDSSIDAFNAVLELDREYSYTYLNRGLAFYYSGRYSE
                     AQRDLLHFYEEDKKDPYRALWLYFNELEFTPTEAKNNLQKRANNLSSDYWGTNIVNYF
                     LGKLNLAQVRAIMERDAKENSSQYAEILTETYFYLAKQQLKLDHKDEAISLFRLALAN
                     QVFNFVEYRFALFELSQIRGMTQTTPVVDKE"
     gene            complement(15801..17942)
                     /gene="pnp"
                     /locus_tag="PXA33_000805"
     CDS             complement(15801..17942)
                     /gene="pnp"
                     /locus_tag="PXA33_000805"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HDL5120156.1"
                     /translation="MVKQFKYGQHTVTLETGAIARQATAAVMASMDDTTVFVTVVAKK
                     DVKEGQDFFPLTVDYQERTYAAGRIPGGFFKREGRPSEGETLIARLIDRPVRPLFPEG
                     FFNEIQVIATVVSVNPQISPDLVAMIGASAALSLSGVPFNGPIGAARVGFINDQFVLN
                     PTTSEQKISRLDLVVSGTDKAVLMVESEADILTEEQMLAAVVFGHEQQQVVIENIKEF
                     VKEAGKPRWDWVAPEPNTDLINKVKALAETRLGDAYRIVEKQVRYEQIDAIKAEVIAQ
                     LTAEDETVSEGTIIDIITALESQIVRSRIIAGEPRIDGRTVDTVRALDICTSVLPRTH
                     GSALFTRGETQALAVATLGTERDAQIIDELTGEKSDRFLFHYNFPPYSVGETGRIGSP
                     KRREIGHGRLAKRGVLAVMPTAEEFPYVVRVVSEITESNGSSSMASVCGASLALMDAG
                     VPIKAAVAGIAMGLVKEDEKFVVLSDILGDEDHLGDMDFKVAGTRTGVTALQMDIKIE
                     GITPEIMRIALNQAKGARMHILGVMEQAIPAPRADISDYAPRIHTMKIDPKKIKDVIG
                     KGGATIRALTEETNTSIDIDDDGTVKIAATDGNAAKAVMARIEEIVAEVEVNQIYNGK
                     VTRVVDFGAFVSILGGKEGLVHISQITNERVERVADYLTVGQEVQVKVVEIDRQGRIR
                     LTMKDINNTNEANAEETVAENVVETEQENNF"
BASE COUNT         5466 a         3421 c         4035 g         5020 t
ORIGIN      
        1 ttattgtcgc tgttacgatt ggagccaata atgatcaact tatcaccttt aactatattt
       61 ttgctcagag tgaattacgc ttatcaacgt tagttgcgat tttatttggc ttaggattga
      121 ttcttggttg gttggtaacg ggtatttttt acttaaaaat taagtttcaa aacgttgctt
      181 taaatcgtcg tgtaaagcgc caagctcagc aaataactga attaacgact ccaaaggctg
      241 aataatcaat gcttgaatta ttgttccttt tattgcctat tgcagcacta tatggctggt
      301 atatgggaca acgtagtgca aagaaggatc aagaaaccgc tagtaataag ttctctcgtg
      361 agtatgttac ggggttaaat tttcttttat ctaatcagca ggaaaaggcg gtggaccttt
      421 tcctctctat gttacaaaaa caagaatccg aaaatcatat tgcatctgaa tcccaatttg
      481 aagcggagtt aaccctaggt aatcttttcc gttcaagagg tgaggtagat cgtgccttac
      541 gtattcacca aacattagtg aatagccctc attattcatt tgatcaaaaa ttattagcaa
      601 aacagcaatt agctaaagac tttatggttg caggtttcta tgaccgtgca gaaaattatt
      661 acatttcatt agttgatgaa cctgagtttg ctaaatattc tttatctcag cttgctgtta
      721 tttaccaaaa aaccagagaa tggaaaaaag cgattaatgt tacagaaaaa cggcttcgaa
      781 tagagccgga tgcagataaa ataccgcttt cacattttta ttgtgaatat gcgcaagggg
      841 ttaaatttga tgataaagcg gcatttattg aatcgttaaa taaagcgtta gaatattatc
      901 ctcaatccac acgcgcttct ataatattag gtgatttcta tttagaagag ggagaatatc
      961 taagagcatt aagctatttc gaacagattc ttgtgcaaga ccctgattat attagcgaag
     1021 tgcttggacg aattaagcaa agctatatgg ctcttgagga cttaaatggc tatgagttat
     1081 ttctcattcg agcaaaccga gtaaaacata atagtagtgt agatattgct ttaactgaat
     1141 ttattgaaga aaaagatggt atcaatgctg cacattctaa actataccag cagttgagta
     1201 cttatccgaa tttaattact tttcaccgct ttattcgcta tcaagcagat tttgcggagg
     1261 aaggaaatgg taaggaaagc ttagttttat tacataatat ggtgggaaat cagataaaac
     1321 gcagcttcca atatcgctgt ttaaattgtg gttatcaaag ttatcgctta atgtggcaat
     1381 gtccttcttg taatcaatgg gaaaaaatta agccggtaca aagtatcgaa ggtattattc
     1441 aataaagaaa gaatctgagg aattgaaaat gaacaataaa gtaattgttg cgttagacta
     1501 tgaaactgaa cgtgaagcat tagaatttgt ggatttggtc gatccgtcgc tttgtcgttt
     1561 aaaagtaggc aaagaaatgt ttacaacctt aggcacaaat tttgtcaaac aattacacga
     1621 acgtaaattt gatgtattcc ttgatttaaa atatcatgat attccaaata cggttgctcg
     1681 agcagtacgt tctgctgctg atcttggtgt gtggatggtt gacttgcatg cttctggtgg
     1741 tttagccatg atggaagagg caaagaaaat tttggagcct tatggcaaag aggcaccatt
     1801 attaattgca gtgacggttc taaccagtat ggaagatttg gatttgcttc aaataggtat
     1861 taatgcttcg ccgatggaac aagttattcg tttagcacat ttagcacaac gggctggttt
     1921 agatggcgtg gtatgttctc cacaagaagt tgaggtatta cgtacccatt gtggtcaaga
     1981 ttttaaatta gtgaccccag gaatccggcc aaaaggaaca gattttgggg atcaacgccg
     2041 agtgatgaca cccaaacaag cagtagaaac aggctcagat tttttggtta ttggtcgtcc
     2101 tattacccaa gctcaagatc cgcttgcggt attaaaatct attaaccaat ctatcagtta
     2161 attcattgca tttttaatga aggcggaaaa atttccgcct tttttaatca aacaagcggt
     2221 tatttttgag gaaaaatttg taaatgacat tagtctattc aacggaaaca ggcagaataa
     2281 agtccgaagt aataaaagaa gaacgcccta aaggggatgg tattgtgcgt attcaacgtc
     2341 aaacaagcgg tagaaagggg aaaggtgttt gccttatcac cggtcttgat ttagaggata
     2401 aagccttaaa ttctcttgct tcagagctta aacgtaaatt gggttgtggt ggctcattta
     2461 aagatggagt aattgaaatc cagggcgata atcgaggatt cattaagcaa atcttagaac
     2521 agaaaggttt caaagtgaaa ctggcaggtg gttgagtttt aaacaattat cagctggcag
     2581 tttttaaata acttcatttt atttgaaact tcattcctac ttttctgcct aaaactcacg
     2641 attaaggaga aaaactaatg tggaagaaaa cgttgattgc tgtattttta gcaagccaat
     2701 atgcttttgc ggtaggaacc gggaaagaac catcaaaaag ttatactgaa acagaattaa
     2761 aacaattaaa aaatgattgg ttagcgattt atcaaaaaga gctacagtat gcagaaaatt
     2821 tgcgtttaaa acagagagaa aattttcagc aaattaatta tttggcagat gcagcgatta
     2881 gtcagaagaa tttatcactt tcaacggagc agataattca gcgtttatta ctaaccttag
     2941 cgggttatcc gttggagatg aaagctgaat ggaagctgct aaatgctaaa ataacgttaa
     3001 aacaagccac agaaagtgaa gttaaagcat ttatttcaaa atatcctgat tctttgtatc
     3061 aaaggcagtt acagaacttg atgtttgagc tgctctatcg tgaacagaaa tttccggagt
     3121 tactggaata tgctcaaaat gtaaaacaga gtagtcttgc cgaccaatgc cgtttatttt
     3181 ctgctcgtta tgaactggca gcaagtaaag ctcaaatcaa tccggaagtt tctcaagtaa
     3241 ctgagcaaaa agcagaaaat gcagaactgc ttgctttggt gcaagagttt gatcgctttt
     3301 ggcttgctac gccgaaatta acttctgatt gtgcgaattt agaaagttat tggcgggatc
     3361 aggggcttaa aaccgatgaa aaaatgcgtt tgaaagcggt cgaattaatt aaacaaaatg
     3421 caaatgcaga attggcgaat ttggcggcaa atgtaaataa tgagaatttg aaaatctggc
     3481 tatcttccgt tgaaaaggta gcgaataatc cggcagattt gcaaaatttt atcgaaaatc
     3541 aaccgcttga ttccccattt tatgctgata ataaagcgat tattcagcaa ctctttgcta
     3601 aatatgtgcg aactttatcg gaaaatatgg agaacccgag tttccagcag tatcaaacgt
     3661 gggcagagaa gtatcaactt tccagcgagg aaatcaatgg ctggaaaaat gcttttatca
     3721 ctcgattttt tgataaccca gaaccaacat tccaattttg gcgtgatgag caaatcaagt
     3781 tgctcaagac agacaattta actgaacgcc gtttacgaat ggcaatttgg caaaaagcag
     3841 atttaaatga gtggctggtt ctgctatctc agcaggcgaa agaaaaagca gaatggcgtt
     3901 attgggctgc gaaagaggag aaaaacgaag cgaagcgtaa agttgatttt gaaagcttag
     3961 ctaaagagag aggcttttat gctatgttgg ctgctcaaca attaggcatt gtttaccaaa
     4021 tcccgatgct tgaagtgcct gaattgacac aagcacagct tgaattgtat gcaaaccaat
     4081 tagctcgtat tcaagagttg cgagagctag ggcagtttga ccaggcaaga aaaatatgga
     4141 ttgactggat aaaaaacatc aagccggaag caggtgaaaa tacaccaact aaaatccagt
     4201 tagccctgat aaaatatgcc aaggataaaa attggtatga tttggcggtg gaaggtacaa
     4261 ttcaaacaaa agcctttgat tatcttgatt tgcgattacc gaatgcctat tcggattggt
     4321 tcgatttaca tctgcaagat aaaaaaatca gcaaaacgtt tgctcaggcg attgctcgtc
     4381 aagaaagtgc gtggaatgct caggctcaat cgcacgctaa tgcgagaggg ctaatgcaac
     4441 tgttaccgac aacagcacaa aaaaccgcta aagataacgg tttaaccttc aaagatggca
     4501 atgatctctt tcaaccgttt aacaatatta tgttaggcac aacccattta atggagttaa
     4561 acgaaaaata tccgaataat cggattctga ttgcctctgc ttataatgca ggagcagggc
     4621 gtgttgagca gtggctgaaa cgtagtaacg ggcagttagc aatggatgaa tttattgcca
     4681 gtatcccgtt ttatgaaacc agaggctatg tacaaaatgt gctggcgtat gactactatt
     4741 atcaaatgct ctatagcgat ataaatgata aaaatggatt aaaaatgttc tatcaagagg
     4801 aattaacaag aaaatattaa aaaatgcttg tgtttttgtg tagaaccgct ataatgcacc
     4861 ccatcagacg cggggtggag cagcttggta gctcgtcggg ctcataaccc gaaggtcgtt
     4921 ggttcaaatc cggcccccgc aaccagtttt aagctcacag ctttggttaa agttgtgagt
     4981 tttgttttat atgctgtagc aaatttgcaa aaaatttggc aaaaaagacc gcttgttgtg
     5041 gttgctacag caaaaaacaa accgcttaaa gccccacggt ttcccgtctg gggctttttt
     5101 gcggaaattg gtacagcatt aacgtatcaa agctggttta tccggctttt tttatatttg
     5161 gagattatga cttggcaact ttagaacaaa aattagaaga tttagtgctc gattctattg
     5221 aagcattagg ctttgaatta gtcggcattg aatgccaacg tgcaggccgt tttttaacgg
     5281 tgcgtttata tattgataaa gaaggtggcg tgacgattga tgattgtagc gatgtcagcc
     5341 gccaagtgag tgcgattttt gatgtggaag atccgattgc cgataaatat aacttagagg
     5401 tttcttcacc agggttagat cgtccattat ttaccgttgc ccactatgag cgttttatcg
     5461 ggcaagatgt agtgattcat ttacgcattc cgatgttcga tcgccgtaaa tggcaaggta
     5521 aaatcaaaca agtagaaggt gatttcatca ctttaacggt agaaaatgaa gatcgccagt
     5581 tcgcatttgg caatattcaa aaagcaaatt tagttccggt ttttaatttt taagataaag
     5641 gtaaactgac aaaatgagta aagagatttt attagctgcg gaagcagttt caaacgaaaa
     5701 gcaattacca aaagaggcaa tttttgaagc cttagaaacc gctttaacca ttgcaaccaa
     5761 aaagagccgt gatttagata ttgacgtgcg tgtggtggta gatcgtaaaa ccggtgattt
     5821 ccaaactttc cgtcgttggg ctgtggttga aaatgtgaat aatatgaccc gtgaaatcag
     5881 cttagaagcg gcacaatttg aagatccaaa cgtacaatta ggcgatttta ttgaagatga
     5941 agtagagtct atcgagttcg gtcgtgtggc gattcagact tttggtcagg tagtcaaaac
     6001 caaaattcgt gaagcggaga agacgaaaat cattgatgaa ttccgttcgg atttaggcaa
     6061 aattattctt gccactgtga aaaaagtaac ccgtgataat attttccttg agttacaagg
     6121 taaatcagaa gatatcaaag gcgaagcggt cattgtgcgt gaagatatga tcccacgtga
     6181 aaacttccgc ccgggtgacc gtgtgcgtgg tgtgttatac gaaatcaagc ctgaatcaaa
     6241 aggacctcag cttttcgtta cccgtgctaa accggtgatg ttagaagagc tattcaaatt
     6301 ggaagtgcct gaaattggcg aaggcgtaat tgagatcaaa ggttcagccc gtgatgtcgg
     6361 ctctcgtgca aaaattgccg tgaaaaccca cgataaacgt attgacccgg taggggcttg
     6421 tgtgggtatg cgtggtgctc gtgttcaagc tatcagcaat gaattaggcg gcgagcgtgt
     6481 ggatatcgta ctttgggacg aaaatccggc tcaatttgtg attaatgcaa tggctccggc
     6541 agatgtaagc tctattgtga ttaacgatga aactcactca atggatattg cggttgaagc
     6601 gaaaaatctg gctcaagcta tcggtcgtaa cggtcaaaac gtgcgtttgg cgacacaatt
     6661 aactggttgg gcattaaatg taatgaccgt tgaagagcta acgaagaaga gcgaagaaga
     6721 agatggtaaa gtcatcaatt tattaacttc tgctttagaa attgatgaag aatttgctca
     6781 agttttaatt gaagagggct ttacgtctct tgaagcgatt gcttatgtgc cggttgatac
     6841 tttaacggcg attgacggtt tagaagatga agatttagtc gaagaattac aatctcgtgc
     6901 caaaaatgcg atcacgaaaa aagcgttaga agaagagaag ttattaaaat cagctcaaat
     6961 tgaagaaaaa ttattaacgc ttgaaggtat ggatcgccat atcgcattca aattagctga
     7021 aaaacagatt acgactcttg aagaacttgc agagcaaggt gttgatgatt tagccgatat
     7081 tgaagaactt tcagaagaga aagctggcga gctaattatg gcagcacgcc aaatttgttg
     7141 gttcaactaa gcaaagagga taataaaatg acagaagaga aaaaaacact ttcgcttggt
     7201 ggcgctcgca aagcgagtaa agtgaataca accacaacca gtggtaaagt aaaagcggtt
     7261 gaagtaaaag agaaaaaacc gaaaattgat gcaaaagcat tgcaagaaaa agcagcaaaa
     7321 gaagctgccg cagcgaaagc tagagctgaa aaagaagcag ctgaaaagtt agccaaagaa
     7381 aaagccgaag ctgaagctaa agagaaagct gcaaaaaaag cagcagaatc gaccgctagt
     7441 aaaccgattt ctgtgccggt aatgccaagc agcaaagcaa aagaagaaaa accgaaagca
     7501 caacaggtac agccaaaaca agagaaagcg gttgatcctg aaaaagaagc taaacgcaaa
     7561 gaagaagctg agttacgccg taagcaagag gaattagctc agcaaaaagc ggaaatggag
     7621 gcaaaacgtg ctgcagaaaa tgcccgccgt ttagcagaaa ttgctcgtga agaagcggaa
     7681 gataacggca acgaagattt tgatgatgag cgttttactt cttcttatgc tagagaggct
     7741 gagcgtgata atgaccgccg tagtgaggga aatcgtggtc gtaacggcaa aggcaatgtg
     7801 gctaaagcga aaaaaggcgg acgtgaagag gataaaaacg aacgcagtgc agatcgtcgc
     7861 aaccataaag aagtaaaagg caaaggtaaa caaggtaaga aaggtgcagc tttacaacaa
     7921 gcctttacca aaccagctca agttgttaaa gctgatgtgg tgattggtga aactatcact
     7981 gtggctgaat tggctaataa aatggcagtg aaagcgactg aaatcatcaa aatgatgatg
     8041 aaaatgggtg aaatggtgac gatcaaccaa gttatcgacc aagaaaccgc acaattagtg
     8101 gcggaagaac ttgggcataa agtgattttg cgtaaagaaa atgagttaga agaagcggtt
     8161 ttagaagatc gtgatactca cgcagaaaaa gtaacccgtg caccggtagt gactattatg
     8221 ggccacgttg accacggtaa aacctcgtta cttgactaca ttcgtaaagc caaagtggcg
     8281 gcaggtgaag caggtggtat tacccagcac attggtgcat accacgttga aaccgatgac
     8341 ggcaagatga tcactttctt agataccccg gggcatgcag cctttacctc aatgcgtgcg
     8401 cgtggtgcga aagcaactga catcgttgtt ctcgtcgttg cggctgatga cggtgtaatg
     8461 ccacaaacta ttgaagctat ccagcacgcc aaagcagcgg gtgcgccatt agtggtagcg
     8521 gtgaataaaa ttgataaacc ggaagcgaac cctgaccgtg tagaacaaga gttactacaa
     8581 cacgaggtta tttctgagaa attcggtggc gatgtgcaat tcgtgccggt atctgcgaaa
     8641 aaaggtatgg ggatcgatga tttactagaa gccattattc tgcaatccga agtgcttgaa
     8701 ttaaccgcag taaaagacgg tatggcaagc ggcgtggtaa tcgaatctta cttagataaa
     8761 ggtcgtggcc ctgtggcaac tatcttggtt caatccggta cgctaaacaa aggcgacatt
     8821 gttctatgtg gttttgaata cggacgtgtg cgtgcaatgc gtgatgaaaa cggtaaagat
     8881 gttgaatcag caggtccgtc tattccggta gaagtattag gcttatcagg cgtgcctgct
     8941 gccggtgatg aggcaaccgt cgtgcgtgat gagaagaaag cccgagaagt ggctctttat
     9001 cgccaaggta aattccgtga agtaaaactg gctcgtcagc aaaaagctaa actggaaaat
     9061 atgttcagta atatggcagc cggtgatgtg gcagaattga acatcattgt taaagcagac
     9121 gtacaaggct cggtagaagc gatcagccaa tccttaatgg aactttcaac cgatgaggtg
     9181 aaagtgaaaa ttgttggttc aggtgtaggt ggtattaccg aaactgatgc aaccttagcc
     9241 gcagcttcta atgctattat ggtcggcttt aacgtgcgtg ccgatgcctc tgcccgccgt
     9301 gtgattgaaa acgaaaatat cgatttacgt tactactcca tcatttatga gctattaaat
     9361 gacgtgaaag cagcaatgag cggtatgtta cagcctgaat tcaaacaaga aattatcggc
     9421 ttggcagaag tgcgcgatgt gttccgccac ccgaaatttg gggcgattgc cggttgtatg
     9481 gtgacagaag gtatcgttaa acgtaacaac ccaatccgtg ttctgcgtga taacgtagta
     9541 atttttgaag gcgagttaga atctctccgc cgtttcaaag atgacgtatc tgaggtgcgt
     9601 aacggtatgg aatgtggtat cggcgttaaa aactacaacg acgtaaaagt tggcgaccaa
     9661 atcgaagtgt ttgaagtggt tgaagtaaaa cgtactattt agcctaattt tgtgtagggg
     9721 cgtattgcat acgccctttg tattttttag ggcgtgaagc atcacgcccc tataaaagag
     9781 aaaaatatgt ccagagaatt taaacgtagt gaccgtgtgg cacaggagct acaaaaagaa
     9841 gtggcggtga ttttacaacg tgaggtaaaa gatcctcgca ttggtatggt gactgtttct
     9901 gatgtggaag tatcaagaga tttggcgtat gccaaaattt ttgtgacttt cttatttgat
     9961 aacgatgctg aagctattaa gcaaggtatg caaggcttgg agaaagcaag cccctatatt
    10021 cgaacccttt taggtaaagc aatgcgtttg cgtattgtgc ctgaactgcg ttttatctac
    10081 gatgaatcgc tagtcgaagg tatgcgtatg tctaaccttg tgtctagtgt aatcaaaaat
    10141 gacgaagaaa aacataaaga ggactaattg tgtctagacc tcgcaaacgt ggccgtgata
    10201 ttcacggcgt tttcttatta gataaaccac aaggaatgtc ttcaaacgat attctgcaaa
    10261 aggtaaagcg aattttccaa gcaaataaag ccggacatac cggggcgtta gaccctctcg
    10321 caaccggaat gctgccgatc tgtttaggtg aagcgaccaa attttcgcaa tttttgttgg
    10381 attcggataa acgttatctc gtcaccgcaa aattaggcga gcgaaccgat acttcagatg
    10441 cggaagggca agtggtggaa accaaagcag ttgcagtgac aaaatctcaa attttggctg
    10501 ctttggagaa atttagaggt gatattttgc aagtgccgac aatgttttcg gccttaaaac
    10561 acaacggcaa acccttgtat gaatatgccc gagcagggat tacggtggag cgtgaggctc
    10621 gcccgattac gatttttgaa ctaaatttca ttgaatacaa ggctccattt ttaacccttg
    10681 aggtgcattg ttctaaaggt acttatatcc gaactttggt cgatgattta ggcgaagagt
    10741 tgggctgtgg tgcacacgtt acgatgctac gccgtacagc agtggcagat tatccgattg
    10801 aaaaaatgat gagctatgca gatttgcaaa atttagccga aaatcaaccg cttgcagagt
    10861 tagaccagca cttattgccg atggatacgg cagtttctgc attagcaaaa ataaatttaa
    10921 gttcagagca aactaaagcg gtgggttttg gtcagagaat tagatttgat aatctgaacc
    10981 aactttatgg tttggtacgt ttatattcag aagatgaaca atttctcggt gtggcagaaa
    11041 tcaccaatga tactgtgatt cgtcctaacc gaatgattaa cttaacggaa taagaatgaa
    11101 atcgataatt tttaccttac ctttagcgat tatcacttta gccttagccg gttgttcaag
    11161 tgataatcaa gaaagttatg ccggcttaaa cttagatgct aaatacagta aatctcgctc
    11221 ggtcagtcat tttaatgatt atgtgcattt tctgaaacag aaggcagcag gggcaggagt
    11281 gtctgcaaaa accttaaatg ctcagaaatt cattcattac aacgccagag cggtgcagtt
    11341 agatcaacag caagctgcac gaagacgtga cccgaatgta ccgcctccgc caccaaatcc
    11401 aagttttaaa caaagtatta actcaagcga aggtggatca ggcaagtgaa cgttggtatg
    11461 aatataatag ccagcttacc catgccagcc aaaaatatgg ggtacaaaaa gagtatattt
    11521 tggcactttg gggaatggag agcagctttg ggcgtaacca aggggatttt gatgtacttt
    11581 cggtgttggc aaccctcgcc tttgatggtc gccgtgagaa gttatttacg caagaatttg
    11641 tgaatgcgat gaaaatgctc gatgacggca caattaaacg ctatgaattg cttggatctt
    11701 gggcaggagc gatgggacaa actcaattta tgccaacttc ttacttaacc tatgcagctg
    11761 atggcaataa tgacggtaaa aaagatattt gggagaatga agccgatgca tttgcctcga
    11821 ttgccaatta tctttcaaca gtaggttggg ataatcaact accttggggt gttgaggtta
    11881 aattagcttc accgatggat ttaagtttcg ccggtcttga aaccaacaaa gcgaaaacat
    11941 tggcggaatg gcaagcctta ggtatttatt tagcttatcc gaatggacaa gaatcggcaa
    12001 aattagctca attacacggc aaagaggttt ggttgattcg tccggataaa gaagcgggaa
    12061 gagcgttttt agtttctaat aatttccgta ctttaatgga ttggaataaa tcgaataacc
    12121 ttggtatcag tatcggaaaa tttgccgatc gaattttagc ttcagttggt caataatcga
    12181 ataaaagcat agtagcaaag cctatctatg cttttatttt atcaaagcgt cgcctttctt
    12241 ctgcaaacga cgttgcttaa aaaaatcact gatcttttgg ctacattctc tgcccaatac
    12301 attgccatgc acttctaaga aatggttcat tttataatct tcaaacagat gatagcgtga
    12361 acctaccgca cctgttttat aatcacttgc cccaaaaacc aatcgcttaa tccgactatg
    12421 taaaattgca ccggcacaca ttgggcaagg ctcgagcgta acataaaggg tacaatctaa
    12481 cagacggtaa ttccctaaag tttgtcctgc ttgacgaatt gcctgcattt cagcgtgtgc
    12541 agatggatca cagagttgaa tggtttggtt ccagcctctg ccgataatct gttggttttt
    12601 atcaaccagt actgccccaa ccgggatttc gccttttgct tcggctaaat cagcgagatc
    12661 aagggcgtat tgcataaagg cgatgtcttg ctcggagatc tgaagtggac aagaagtttg
    12721 atttggtttg ataaacatag ttgcaaaaaa ttgagttttt ttgaccgctt gtaaaatgcc
    12781 ccacgagggt ggggcatcgc ttacttcgct tagtgtcgtt tagtcacgac gaccacgttt
    12841 ttcactgaaa cgtttttctt taaagccacc tttgcggtct ttattgaaac ccccgtctcg
    12901 gcgaccacga ccttcaaatg gatttgcatt acttgcactg tttgcagagc caagtaatgt
    12961 catctgcatt ggtttgctta atacacgtgc tttgtgagcg aaatgttgta cgagatgatt
    13021 tggcattcct tgcggcaact caatggttga gtatttatca taaagtttga tatgcccgat
    13081 gtaacggctg ctgatatccc cctcgttggc aatcgctcca acaatatgac gaacttccac
    13141 accgtcttca cgccccaatt caatgcggta taaatccatt gccacaccat tgttttcacg
    13201 gtgttcacga ccgttacgac gatcgtttcg ctcattgcgg tcacgtccac gttcagaacg
    13261 ggcagcacgc acaaccggat ccggtggcag aatcagtttt tgtttttctt gcagtaacat
    13321 catcatcgcc gctgctaatt cttcgtggtc ttgatccgca gtaaataaat cttccaacaa
    13381 ttcacgatat ttttctaaat cgtgatgttc taactgctta gaaatgcggg ctttgaattt
    13441 ctcacggcgt ttttccatta aaatttcgtg gtttggaatc gccacttcat taattggttt
    13501 tttgattaaa ttttcgatat tgcgtaataa gcggcgttca cgaggctcaa caaacagtaa
    13561 ggcacgacct gaacgaccgg cacgaccggt acgtccgatg cggtgaacat aagattcttc
    13621 ttctaacgga atatcgtagt taataaccaa gctgattcgc tcaatatcaa taccacgggc
    13681 agcaacatcg gtggcgacta aaatatctaa acgaccggag cgtaatttat ctaaggtttg
    13741 ctcacgggct tgctgggtca tatcgccgtt taatgccgca gtacggtagc cgttgcgttc
    13801 acacaattcg gtaatatcaa tagtaccggt tttggtgcgg gtaaagatga tagcagcatc
    13861 aaattcttct acttctagga aacgcaataa cgcatcgttt ttacggaagc cgtttaccag
    13921 ccagtagctt tgttcaatat ccggggctga acgctcggtt gccttaattt ttacctcttg
    13981 cggatctttc ataaaacgac gggtaatacg gcgaatcggc tccggcatgg tggcagagaa
    14041 gagagcagtt tgatgtgttt ccggtaattc agccattacc gtttcaacat cgtcaataaa
    14101 gcccatacgc agcatttcat cggcttcatc taacacgatg aattttaatt cggataaatc
    14161 taatgtgcca cggcggatat ggtcaagaat acgcccaggt gtacccacta ctacttgtga
    14221 gccttgtttt aaagcacgta attgaatgtc gtaacgttgt ccaccataaa ccgtcactac
    14281 acgaatgcct ttcatatttt ttgcaaactg ctcacaagca tcggctactt gaatcgcaag
    14341 ctcacgggtg ggtgccatca ccaacatttg cgggtggcgt tgtgttgggt caatttgtgc
    14401 caacagcggc agcgagaatg cggcggtttt accgctcccg gtttgtgcca taccaagcac
    14461 atcacgaccg gctaataaat gaggaataca ttcttgttga atgggtgatg ggtttacaaa
    14521 gcccatttca tttacagcat caaggattgg ttgaggtaag cctaaatcgg cgaaagttaa
    14581 agattcagtt gtcataaaaa accatttgtt tggaacgcac aaagcagaaa atatcgacta
    14641 tttttctgca cagccattaa tgaaaattat attgttgaaa tttaggaaga aatgaaataa
    14701 caaagcaagt gatgctttaa aaatcccctg agtataagcg gttaaattta caaaattttt
    14761 tgcgaaatta accgcttgta ttgtcctaat ctttccctat tctttatcaa caacgggagt
    14821 cgtttgagtc ataccacgaa tttgtgataa ctcaaacagg gcaaaacgat actcaacgaa
    14881 gttaaacact tgatttgcaa gagcaagacg aaagaggctg attgcctcat ctttatgatc
    14941 taatttgagt tgttgttttg ctaagtagaa atacgtttct gtcagtattt ctgcatattg
    15001 tgagctattt tccttagcgt cacgttccat tatagcacga acttgagcta aattgagctt
    15061 ccctaaaaaa taatttacga tatttgttcc ccagtaatcc gaagaaagat tattagctcg
    15121 cttttgtaaa ttatttttag cttctgttgg tgtaaattcc agctcattaa aatagagcca
    15181 taatgctcga taaggatctt tcttatcttc ttcataaaaa tggagaagat cacgctgggc
    15241 ttcagaatat ctgccgctat aatagaaagc caaacctcga ttgagatagg tgtagctata
    15301 ttcccgatct aattccaata cggcattaaa cgcatcaatc gaactgtcgt aatcgccttc
    15361 cagcagcaga tacagcccca tatagttata agctgccgcc atacgttgat tgagcgaaat
    15421 ggcttgagca aaatcataac gggctaacga ccataaacct aaactgtcgt aaataacgcc
    15481 tctttcaaaa tagaggtctg cttgctcaga gggcgttaat ggggcttctt ccaatacttg
    15541 gctgagtctt accaccattg cctcttgttc aaagcgaagt tgaggattaa gttctgccaa
    15601 tgctaagttt ttaattgaaa ccaaatcggc ataccgactg gtacagccgc ttagcattaa
    15661 aacagaaatt aaattcagta ataagaaacg gattcgaaac aacttaaaaa gttgtaccat
    15721 tacagcatat ccctcaatta aggaaaatta aaaactgaat aacggacact caaagtccgt
    15781 tattcgcctt tattattgaa ctagaaatta ttttcttgtt ctgtttctac cacattttca
    15841 gctacagttt cttctgcatt tgcctcgttg gtattattga tgtctttcat cgtcagacga
    15901 atgcgtcctt gacggtcaat ttctaccact ttcacttgta cttcttgacc aacggttaag
    15961 tagtccgcta cacgctcaac acgttcgttg gtgatttgtg aaatgtggac taaaccttct
    16021 ttgccaccta agatggaaac gaatgcaccg aagtccacca cacgggttac tttaccgttg
    16081 tagatttggt ttacttccac ttcggcaacg atctcttcaa tacgagccat tactgctttc
    16141 gctgcattgc cgtcagttgc cgcaattttc accgtaccgt catcatcaat gtcgatagaa
    16201 gtattggtct cttcggttaa agcacgaatt gttgcaccgc ctttaccaat cacatctttg
    16261 attttcttcg gatcgatctt cattgtgtga atgcgtgggg catagtcaga aatatctgca
    16321 cgaggtgccg gaatggcttg ttccattaca cctaaaatgt gcattcttgc acctttagct
    16381 tggtttaagg caatacgcat aatttcaggg gtgatccctt cgattttgat gtccatttgc
    16441 agcgcagtca caccggtacg ggttcccgct actttaaagt ccatatcgcc taagtggtct
    16501 tcatcaccta agatgtcaga aagtaccacg aatttctcgt cttctttcac gagccccatt
    16561 gcgataccgg caaccgctgc tttgattggc acacctgcgt ccatcaacgc aagagacgca
    16621 ccacacacag atgccattga agaagaaccg ttagattcag tgatttcaga caccacacgc
    16681 actacatacg ggaactcttc agcggttggc atcacggcta atacgccacg ttttgctaaa
    16741 cgaccgtgac cgatttcacg gcgttttggc gagccgatac gaccggtttc gcccacagag
    16801 tatggaggga aattgtagtg gaataagaaa cggtcagatt tttcgccggt caattcgtca
    16861 atgatttggg catcacgctc tgtgcccaat gttgctactg ctaatgcttg ggtttcgcca
    16921 cgggtgaaaa gagcagaacc gtgggtgcgt ggtaacacac tggtgcaaat atccaatgca
    16981 cgcacggtat ccaccgtacg gccgtcaatg cgtggttcgc ctgcaataat acggctacgc
    17041 acgatttggc tctctaatgc ggtgatgatg tcgatgatag tcccttcaga aacagtttca
    17101 tcttctgcgg taagttgtgc aatcacctct gctttaatcg catcgatttg ctcgtaacga
    17161 acttgttttt ctacgatacg ataagcatcg ccaaggcgtg tttctgctaa tgcttttact
    17221 ttgttgatta aatctgtatt tggctctggt gcaacccaat cccaacgtgg tttgcccgct
    17281 tctttaacaa attctttgat gttttcgatt acaacctgtt gttgctcgtg gccgaacacc
    17341 accgccgcta acatttgctc ttcggttaag atatccgctt cagattccac catcaacacg
    17401 gctttgtctg tacctgaaac cactaaatct aagcggctga ttttttgctc gctggtggtt
    17461 gggtttaata cgaattgatc gttgataaaa ccgacacgag ccgcaccgat tggaccgtta
    17521 aacggcacgc cggataatga aagggcagcc gatgcaccga tcatcgcaac cagatcagga
    17581 ctgatttgtg ggtttaccga tactacggtc gcaatcactt gaatttcgtt aaagaaacct
    17641 tctgggaaaa gtggacgcac aggacggtcg atcaagcgag cgattaaggt ttcaccttcg
    17701 ctaggacgtc cttcacgttt gaagaaaccg cccggaatac gaccggctgc gtaagtacgc
    17761 tcttgataat ctacggttaa tgggaagaaa tcttgccctt cttttacgtc ttttttcgct
    17821 actacggtaa caaatacggt tgtgtcgtcc atacttgcca ttactgctgc cgttgcttgg
    17881 cgtgcgatag caccggtttc taaggtcacg gtgtgctgac cgtatttaaa ctgttttaca
    17941 at
//
LOCUS       DANLRK010000033        17477 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.033, whole genome shotgun sequence.
ACCESSION   DANLRK010000033 DANLRK010000000
VERSION     DANLRK010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 17477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17477
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.033"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            <1..245
                     /locus_tag="PXA33_000806"
     CDS             <1..245
                     /locus_tag="PXA33_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL5120157.1"
                     /translation="VVFQRKALKQLRKIPTGAEIRRKCDDLAYFPYCANIKALTNHQY
                     DYRFRVGNYRIFFNIEGETMNIVSIEEVKKRDERTY"
     gene            229..576
                     /locus_tag="PXA33_000807"
     CDS             229..576
                     /locus_tag="PXA33_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015702293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL5120158.1"
                     /translation="MKELTNIQYLNNEEGKPAFAVLPIATLEWLKQKANIKTAIETGI
                     PSAVAGIALDNNYSALRAWREYLGLTQEEVAQRLGISQSAYSQHENAKNLRKTTRIKI
                     AYALGLNEEQLDF"
     gene            complement(617..1777)
                     /gene="trmG/rlmN"
                     /locus_tag="PXA33_000808"
     CDS             complement(617..1777)
                     /gene="trmG/rlmN"
                     /locus_tag="PXA33_000808"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HDL5120159.1"
                     /translation="MSELITNAGCGSAEQPKEKINLLNLNRQEMRELFAEMGEKPFRA
                     DQLMKWIYHFGEENFDNMSNINKVLREKLKRIAEIKAPEVAVEQRSADGTIKWAMQVG
                     DQQIETVYIPEDDRATLCVSSQVGCALACTFCSTAQQGFNRNLSVAEIIGQVWRASKI
                     IGNFGVTGVRPITNVVMMGMGEPLLNMNNVIPAMEIMLDDFAYGLSKRRVTLSTSGVV
                     PALDKMREQIDVALAISLHAPNDELRDELVPINKKYNIKMLMDSVNKYREVSNANHGK
                     VTIEYVMLDHVNDSTNHAHQLAEVLKNTPCKINLIPWNPFPEAPYGKSSNSRVDRFQK
                     TLMEYGFTVTVRKTRGDDIDAACGQLAGDVIDRTKRTLEKRKFGQGIAVKTH"
     gene            2037..2933
                     /locus_tag="PXA33_000809"
     CDS             2037..2933
                     /locus_tag="PXA33_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HDL5120160.1"
                     /translation="MKLSTIKKALVLTFGLSAAMAMAEPFKVVTTFTVIQDIAQNVAG
                     DKATVESITKPGAEIHDYQPTPKDIVKAQKADLVLWNGMNLERWFERFFENVKGKPAV
                     VVTEGITPMPITEGEYKNLPNPHAWMSTDNALIYIENIRAALVKYDPQNADTYNANAK
                     AYAEKVKGIAEPLRQRLAFIPEQQRWLVTSEGAFSYLAKDYNLKELYLWAINAEEQGS
                     PKQVKKVIDGVKANKIPVVFSESTVSDKPAKQVAKETGALYGGVIYVDSLSEKDGPVP
                     TYLDLLKVTIGTIVEGFERSKK"
     gene            2946..3689
                     /locus_tag="PXA33_000810"
     CDS             2946..3689
                     /locus_tag="PXA33_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HDL5120161.1"
                     /translation="MTTVSASISVENLSVRYNNGHIALQDVSFHLENGTICALIGVNG
                     GGKSTLFKSLMGLVKPLTGSVKLSQLPIAKALKQNLVSYVPQSEEVDWQFPVSVYDVV
                     MMGRYGYMNFLRRPSQTDKLKVEQAMQRLDVLHLKDRQIGELSGGQKKRVFLARALAQ
                     ESKIILLDEPFTGVDVKTENAIVELLKQLKNEGHLILVSTHNLASVPSFCDQVLMVNR
                     TLLAKGKTEEIFNNQNLERVFGGLLHYQK"
     gene            complement(3746..4555)
                     /locus_tag="PXA33_000811"
     CDS             complement(3746..4555)
                     /locus_tag="PXA33_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDL5120162.1"
                     /translation="MLNWILEPLQFEFMQIALITAILVAGVSAVLSCYLVLKGWSLMG
                     DAISHAVLPGVVISSLAGLPLSIGAFFSGLGCALSVGYLKENSRLKEDTVMGIVFSGM
                     FAFGILLFTAFNTGEHLTHILFGNLLGVTKSDLLQTLLISLFTFVVMVAKRKDFLLYC
                     FDVNQAKVAGLPVRLLHYGLLSLLALTIISAMQVVGVILVVAMLITPGITAYLLTKRF
                     DSMLLIALGVAIFSSVFGTLLSYHIDAATGPTIILVQAAIFFLSFAYSKIH"
     gene            complement(4548..5408)
                     /locus_tag="PXA33_000812"
     CDS             complement(4548..5408)
                     /locus_tag="PXA33_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDL5120163.1"
                     /translation="MLDLLLEPFTYNYMQKAIFVSMGVGLVCAFLSAFLMLKGWSLIG
                     DALSHAVVPGVAIAYALKLPYAFGAFFSGILAALSILWVKSLTKIKEDAVIGFIFTTF
                     FALGMFIVSLNPTSVDVNSIVMGNILGIADDDLWQVVLIIAISLLGLILFWKDLLLVF
                     FDEHHALSVGLSPLRYKVIFFTLLSACVVVALQTVGAILVIAMVVTPGATAYLLTDRF
                     PRLVLIAMLIGTLTSGIGAYLSYFLDGATGAVIVCLQTLIFLFAFCFAPKYGLITQKR
                     KLHRVEVQNA"
     gene            complement(5482..6267)
                     /locus_tag="PXA33_000813"
     CDS             complement(5482..6267)
                     /locus_tag="PXA33_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HDL5120164.1"
                     /translation="MMKKITILGLGWLGLPLAEALQNKGWRVAGTKRTVGELAIECYP
                     LDLNALAVTSEIEQLLAADALVIALPLSQIRPENYGCGIQTLVSLAIEKGLKHLVFTS
                     STSVLPMKSGVFDENAVVDSANLLVKMENWLLSQPIHCDILRLAGLVGKKRHPVFYLA
                     GKQNLSGAGQPVNLVHLEDCIAAISLLLEQPNGQRIFHLCAEQHPSRKDYYSEMAKRF
                     GLADLHFSEENQPLVRLIKADKICQELGFRYYYPDPYQFKVDI"
     gene            complement(6355..6430)
                     /locus_tag="PXA33_000814"
     tRNA            complement(6355..6430)
                     /locus_tag="PXA33_000814"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6395..6397),aa:Gly,seq:gcc)
     gene            complement(6457..6543)
                     /locus_tag="PXA33_000815"
     tRNA            complement(6457..6543)
                     /locus_tag="PXA33_000815"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6507..6509),aa:Leu,seq:taa)
     gene            complement(6562..6637)
                     /locus_tag="PXA33_000816"
     tRNA            complement(6562..6637)
                     /locus_tag="PXA33_000816"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6602..6604),aa:Gly,seq:gcc)
     gene            complement(6803..8041)
                     /gene="pepT"
                     /locus_tag="PXA33_000817"
     CDS             complement(6803..8041)
                     /gene="pepT"
                     /locus_tag="PXA33_000817"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HDL5120165.1"
                     /translation="MKQSLLNRLLRYTQIHTTSDPKSQTVPSSQQQWDLLHLLKTEVE
                     AMGLSEIYLDPNGYLFASLPANIDKTLPVLGLIAHVDTSPDFNGENVKPQVIEAYQGG
                     NILLNGSGDYLSPTDFPNLNKLIGDTLVTTDGTSLLGADDKSGIAIILSAIEYLQANP
                     HIPHGKIRIGFTTDEEIGRGADHFDVNAFGADVAFTFDGGPLGELQYENFNADAATVT
                     FFGRSVHPGTAKGKMINALERACEFQHRLPPHLTPATSEGREGFIMLHGIEGGIDKAE
                     IHYILRSFERDELAAFGKMLNDTLAEMQQIYGEHCGKVEIVEQYRNMREVLDDYGYLV
                     EIAENAMREQGIEPNLEPIRGGTDGSRLSFMGLPCPNLFTGGENFHGRFEFISVDTME
                     KATKVVIDICRKFADYDFNK"
     gene            complement(8061..8450)
                     /locus_tag="PXA33_000818"
     CDS             complement(8061..8450)
                     /locus_tag="PXA33_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HDL5120166.1"
                     /translation="MYMLDTNTVSYFFRGNANVVAKLKSINPERLCISSVTAAELVYG
                     VAKRNNGQLSQFLDYFLSSVKVLDWNYRCAELYGKLRAEMEKNGKVMGVQDQMIASHA
                     LAEECILVSSDQAFKMVPDLKLENWLH"
     gene            complement(8451..8711)
                     /locus_tag="PXA33_000819"
     CDS             complement(8451..8711)
                     /locus_tag="PXA33_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HDL5120167.1"
                     /translation="MEKIARLFRNGRNQAVRLPVEFEFDAEQVYVSKKENGDILLSLR
                     SRNTEHWQRLFELLPAVVCDETFLDTKERNQSFEQRDPFEVA"
     gene            complement(8827..9039)
                     /locus_tag="PXA33_000820"
     CDS             complement(8827..9039)
                     /locus_tag="PXA33_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HDL5120168.1"
                     /translation="MRDEYDFSNAVKNPYAKGLKSKSVVTIRIEDDVISYFKQLAEEV
                     NLPYQTLINLYLKDCVEQKRKPTISW"
     gene            complement(9040..9327)
                     /locus_tag="PXA33_000821"
     CDS             complement(9040..9327)
                     /locus_tag="PXA33_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HDL5120169.1"
                     /translation="MEEINGFEWDSEKALRNQQKHGVSFIEAVSVFYDNNAMLIPDPD
                     HSSREERFLLLGRSNKDNLLVVVHCERNTNIRIISARLATKRESGQYKGVH"
     gene            complement(9490..13023)
                     /gene="mfd"
                     /locus_tag="PXA33_000822"
     CDS             complement(9490..13023)
                     /gene="mfd"
                     /locus_tag="PXA33_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HDL5120170.1"
                     /translation="MSLISLNLPKSDGNFQDHQTLGNLVGHSDTLAISQAARAFYGLT
                     VVVTPDTRTALRLEKALPQFAGLPVQLFPDWETLPYDNFSPHQDIISARLSALFELQQ
                     GRQQILLLPINTLMQKVCPPNYLANNVLLIKKGDRFSIQKLRLQLENAGYRAVEQVLE
                     YGEYAVRGSILDLFPMGAEEPFRLDFFDDEIDSIRTFDVDNQRTKTEIAEINLLPAHE
                     FPTDSNGIEHFRAKFREKFGEIRREPEHIYQQVSKGILNAGIEYWQPLFFEEMASLFD
                     YFPAQTLFITFADIQQKAEQFHQDTQNRYESHRVDPMRPLLPPSDLWFAIDEVNRWLK
                     GYPRLTITSEKIRKSAAKMNANVANLPDIAIQSSAKEPFSAFQQFRQKFDGNILFSVE
                     SEGRRETLLDLLAPLGVKPKQVNSLDEAQGLPFSLIISPLDQGFVVETGGHGTRPYDD
                     ELNTNGENDNNYVGAGPVPARNLAIICETDLLGERVQTRREKNRKAVNPDTLIRNLAE
                     LKIGQAVVHLENGVGRYGGLTVLDAGGIKAEYLVLHYANDAKLYVPVASLHLISRYIG
                     GADETAPLHKLGSEAWAKTRQKAAEKIRDVAAELLDVYAKRESQTGFAFAYDKAEFDQ
                     FSATFPYEETDDQKIAINAVISDMCQAKAMDRLVCGDVGFGKTEVAIRATFLAVMNHK
                     QVVILAPTTLLAQQHYENFKDRFANYPVNVEVLSRFKTAKEQKTILQNVAEGKVDILV
                     GTHKLLQDDVAFRDLGLLVIDEEHRFGVRQKEKIKQLRANVDILTLTATPIPRTLNMA
                     LNGMRDLSIIASPPARRLLIKTFVRQSDEMVIREAILREILRGGQVYYLHNDVATIEN
                     CAEKLAELVPEARIVIGHGQMRERELERVMSDFYHQRFNLLVCSTIIETGIDVPTANT
                     IIIERADKFGLAQLHQLRGRVGRSHHQAYAYMLTPHPKTLTKDAEQRLEAMSTIDNLG
                     AGFALATHDLEIRGAGELLGSEQSGQIESIGFSLYMDLLENAVKALQEGREPSLDEIT
                     QHQAEIELRVPALLPDDYLPDVNMRLSFYKRIASSENLQSLNDLKVELIDRFGLLPEA
                     TKNLFAITQLRLSAQTLGIKKIDAGIHGGYLEFKPTAQPDPMKFIALIQKQPAVYSFE
                     GAVKFKFRLALEDNRKRLEFVENLLREVVGG"
     gene            13184..14401
                     /locus_tag="PXA33_000823"
     CDS             13184..14401
                     /locus_tag="PXA33_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter"
                     /protein_id="HDL5120171.1"
                     /translation="MKNKILGSALMIAGTTIGAGMLAMPLTSAGMGFGATALLLVGLW
                     ALLAYTGLLFMEVYQTAPQKDVGVASLAEQYFGLIGRVLATFSLLVLLYALLAAYITG
                     GGSLLAGVMPEMADADMKLKISILIFTAVLGTFVVVGVKSVDGLTRVLFLGKIVAFIA
                     VLLMMLPKAKLENLTAIPLDNLLIISAIPIFFTAFGFHVIMGTINSYLDADIGKIRKS
                     IYIGTAIPLVAYLLWQLATHGVLSQNEFTTILTQDPTLNGLVKATSQITGSTILGEMV
                     RLFSVLALITSFLGVSMGIFEGVGDLLKRLNLPTNRLWLTIATFTPPILFALFYPNGF
                     IKALGYAGLLFAFYGMILPIGLAWKARRQYPNLPYRVIGGNLALVVALIAGIIIMVIP
                     FLIQAGYLPQAVG"
     gene            complement(14481..14987)
                     /gene="hutX"
                     /locus_tag="PXA33_000824"
     CDS             complement(14481..14987)
                     /gene="hutX"
                     /locus_tag="PXA33_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme utilization cystosolic carrier protein
                     HutX"
                     /protein_id="HDL5120172.1"
                     /translation="MISIEQQTALRNELAENPGQILEMLAAKYQCTLEEVMLNLPAEM
                     LKVTEGERFAEVLQQIHEWDDAITLISHTEDTIIEFVGKLPSGSISRGFYNFEHKEGG
                     GLQGHLRYENCAKIYLLDRPFMGKRTVSLIFINKKGNAMFKIFVGREKIGGALKEHQI
                     KALYQLIG"
     gene            complement(15042..17120)
                     /locus_tag="PXA33_000825"
                     /pseudo
     CDS             complement(15042..17120)
                     /locus_tag="PXA33_000825"
                     /inference="COORDINATES: protein motif:HMM:TIGR01786.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent
                     hemoglobin/transferrin/lactoferrin family receptor"
     gene            complement(17087..17470)
                     /locus_tag="PXA33_000826"
     CDS             complement(17087..17470)
                     /locus_tag="PXA33_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HDL5120173.1"
                     /translation="MVFTSYSIKSKKLCPTILALSVSSILSSQATYAEETTAELSEVN
                     VVAKEDVKASIEKKNKEIIQQELIQNNRDLVRYSPDVGIVNQGRHQKGFAIRGVEDNR
                     VGISIDGVSLFLIQKKIPFINAMVI"
BASE COUNT         5066 a         3931 c         3480 g         5000 t
ORIGIN      
        1 tagtggtatt ccagcgtaaa gccttaaagc aattacgaaa aatcccaact ggtgcagaaa
       61 ttcgccgaaa atgtgatgat ttagcttact ttccctactg tgccaatatt aaggcattaa
      121 caaatcatca atatgattat cgctttcggg tcggaaatta ccgtattttt ttcaatattg
      181 aaggcgaaac gatgaatatt gtgtctatcg aagaggttaa aaaacgagat gaaagaactt
      241 actaatattc aatatttaaa taatgaagaa ggaaaacctg cctttgccgt acttccgata
      301 gcaacacttg aatggcttaa gcagaaagca aatattaaaa ccgcaataga gaccggtatt
      361 ccatctgctg ttgctggaat tgcattagat aataactact cagctctgcg tgcttggcgg
      421 gaatatttag gtttaactca agaagaggtg gcacaacgct tggggatttc gcaatcagct
      481 tatagccaac acgaaaatgc aaaaaatcta cgaaaaacga cccgcataaa aattgcctat
      541 gcgttgggtt taaatgaaga gcaattagat ttctaataac aaaaaggcag gaacctctgc
      601 cttttcacta ttcagattaa tgggttttaa ccgcaatacc ttgcccgaat ttacgttttt
      661 caagggtgcg tttggtgcgg tcaatcacat cgccagcaag ctgtccgcaa gcggcatcaa
      721 tatcatcacc tcgtgtttta cgcactgtta ccgtgaaacc gtactccatc aaggtttttt
      781 ggaaacgatc tacccgagag ttagagcttt taccataagg agcttctggg aacgggttcc
      841 acggaatcaa gttaatttta cacggcgtgt tttttaatac ttcagcaagt tggtgggcgt
      901 ggtttgtgct gtcattgaca tgatctaaca tcacatattc aatcgtgacc ttgccgtggt
      961 tggcgttaga aacttcacgg tatttattca ccgaatccat caacattttg atgttgtact
     1021 ttttattaat cggcactaat tcatcacgca gttcatcgtt cggagcgtga agtgaaattg
     1081 ccaacgccac atcaatttgc tcacgcattt tgtctaatgc cggcaccaca cccgaagtgg
     1141 aaagcgtgac acgacgttta gataagccgt aagcaaaatc atctaacatg atttccattg
     1201 ccggaatcac gttattcata ttcagcaatg gttcacccat tcccatcatg accacattgg
     1261 taatcgggcg aacaccggtc acgccaaaat tgccgataat tttagaagca cgccacactt
     1321 gcccgataat ttccgccacg cttaagttac ggttaaagcc ttgttgagcg gttgagcaga
     1381 aggtacaagc caaggcacag cctacttgtg aagaaacaca cagggttgca cggtcatctt
     1441 caggaatata taccgtctcg atttgctgat cgcccacttg cattgcccat ttaatcgtac
     1501 cgtctgcaga acgttgttct accgccactt caggggcttt aatttcggca atacgcttta
     1561 atttttcacg caatacttta ttgatattgc tcatattatc gaaattttct tcgccaaaat
     1621 ggtagatcca tttcattagc tggtcggcac ggaaaggttt ttcccccatt tctgcaaaca
     1681 gttctcgcat ttcttgacga ttaagattta ataaattgat tttttctttt ggttgttcag
     1741 cagacccaca gcctgcattg gtaatcagtt ctgacataaa gttgaaagcc tcgttattac
     1801 acggattacg gcaaaaggtt agggcaaatg cccttatttt gcgattggat tttaaagcgt
     1861 gcaattttac tggctcttga gggattaggc tagtaatttt taatcaaaag agttgaattg
     1921 cctgagtaaa cttatcaaga ataaccttat aaaaacaaga ctatttacat cattcccaat
     1981 gttctataga atagctttcg ataattctca ttctcaataa caaaaggagg ctaaaaatga
     2041 aactatcaac aattaaaaaa gcattggtat taacctttgg tttaagtgca gcgatggcaa
     2101 tggcagagcc gtttaaagta gttaccactt ttaccgtgat tcaagatatt gcacaaaatg
     2161 tcgccggcga taaagccact gtggaatcca ttactaaacc gggtgctgaa attcacgatt
     2221 atcaaccaac accaaaagac attgtaaaag ctcaaaaggc agacttagtg ttatggaacg
     2281 gtatgaacct tgagcgttgg tttgaacgtt tctttgaaaa tgtaaaaggt aaaccggcgg
     2341 ttgtcgtcac cgaaggcatc accccaatgc cgattaccga aggtgaatat aaaaatctac
     2401 caaatccgca cgcttggatg tccaccgata acgcattaat ttacattgaa aatattcgtg
     2461 cggctttagt aaaatatgac ccacaaaacg cagacaccta taatgccaat gcaaaagcct
     2521 atgctgaaaa agtaaaaggt attgctgagc cactccgcca acgtttagct tttattcctg
     2581 agcagcaacg ttggttagta accagtgaag gggcatttag ctatttagcg aaagactaca
     2641 acttaaaaga actttattta tgggcaatca atgcggaaga gcaaggttcg ccaaaacaag
     2701 tgaaaaaagt gattgatggt gtaaaagcca acaaaatccc tgttgtgttt agcgaaagta
     2761 ccgtttccga caaaccggca aaacaagtgg cgaaagaaac cggtgcatta tacggtggcg
     2821 taatttatgt ggattcactt tcagagaaag atggtccggt tccaacctat ttagatttgc
     2881 ttaaagtgac tatcggcacc attgtcgaag ggttcgaacg aagcaaaaaa taataagatt
     2941 tagcaatgac aactgtttca gcctccattt ccgtcgaaaa tttatcggtc cgttataata
     3001 acggacatat cgctttgcaa gatgtctcgt ttcatctgga aaacggcaca atttgtgcat
     3061 taatcggcgt gaatggaggt ggaaaatcca ctctgtttaa aagtttaatg gggctggtta
     3121 agccattaac cggctctgtt aagctgagtc agctcccgat tgctaaagcc ctcaaacaaa
     3181 atttggtttc ctatgtgcca caaagtgaag aagtggactg gcaatttcca gtttcggttt
     3241 acgatgtggt aatgatgggg cgatacggct atatgaattt tctacgccgc ccaagccaaa
     3301 ccgataagct caaagtagag caagcaatgc aacgccttga tgttttacat ctcaaagaca
     3361 ggcaaatcgg cgaactttcc ggcgggcaaa aaaaacgggt atttttagcc cgtgccttag
     3421 ctcaagaaag taaaatcatc ttacttgatg agcctttcac cggtgtggat gttaaaaccg
     3481 aaaatgcgat tgttgagcta cttaaacagc ttaaaaatga ggggcatttg attttagttt
     3541 ccacccacaa ccttgccagc gtaccaagct tttgcgatca ggttttgatg gtcaatcgca
     3601 cgctactggc aaaaggtaaa acggaagaaa tctttaataa tcaaaattta gaacgggtgt
     3661 tcggcggttt attgcattat caaaagtagt gtttcagcaa caaatcacaa acaaataggg
     3721 aggcaaatgc ctccctattt ttgtatcaat gaatcttaga atatgcaaaa ctgaggaaaa
     3781 aaatggccgc ttgcacgaga ataatcgtcg gccctgttgc ggcatcaata tggtagctaa
     3841 gcaacgtgcc gaataccgat gaaaatatcg caacgcctaa ggcaatcagt aacatactat
     3901 caaaccgctt agtcagcagg taagcagtga tccccggggt aatcaacatt gccaccacta
     3961 aaatcacgcc caccacttgc attgcactga taatagttaa agcaagcaag gaaagtaaac
     4021 cataatgcaa taatctaacc ggtaagcctg ctactttggc ttgattaaca tcaaagcaat
     4081 agagcaagaa gtctttgcgt ttcgccacca tcaccacaaa ggtaaagagg cttatgagta
     4141 aagtctgaag caaatcgctt ttggttacgc ctaatagatt accaaataaa atatgggtta
     4201 aatgctcacc ggtattaaag gcggtaaaca gcaagatccc aaaggcaaac atacctgaaa
     4261 acacaatccc cattaccgtg tcttctttca agcgactatt ttcttttaaa tagcccaccg
     4321 ataaggcaca gcctaatcct gaaaaaaacg cccctattga gagtggtaac ccggcaagtg
     4381 aggaaatcac cacaccgggc agaacagcgt gcgaaattgc atcgcccatt aaagaccagc
     4441 ctttcagcac cagatagcaa gataaaaccg cactcacgcc agcaactaaa attgcggtaa
     4501 ttaacgcaat ttgcataaat tcaaattgca acggctctaa aatccaatta agcattttga
     4561 acctccacac ggtgcaattt ccgcttttga gtaatcaagc catatttagg agcaaagcag
     4621 aatgcgaata agaaaattaa ggtttgtaag cacacaatta ccgcaccggt tgcaccgtct
     4681 aaaaagtagc ttaaatatgc cccaatccct gatgtcagcg tgccgataag cattgcgatc
     4741 agcactaatc tagggaaacg gtcggtaagt aaataagcag ttgctcccgg cgtgacgacc
     4801 atcgcaataa ccaaaattgc cccaaccgtt tgcagagcca ctactacaca ggcacttaat
     4861 aaggtaaaga aaatcacttt atagcgtaat ggcgaaagcc cgactgacaa tgcgtggtgt
     4921 tcgtcaaaaa agacgagtag caaatccttc caaaatagga ttaaacccag taacgaaatc
     4981 gcaataatca gtaccacctg ccacaagtca tcatcagcaa tgcctaaaat atttcccatt
     5041 acaatcgaat taacatcaac cgatgttgga ttgagggaaa caataaacat accaagcgca
     5101 aaaaaagtcg tgaaaataaa gccgataacc gcatcttctt taattttcgt caggcttttt
     5161 acccaaagaa tcgataatgc cgccaaaatc ccggaaaaaa acgcaccaaa ggcgtagggc
     5221 aattttagtg cataagcgat ggcaacacct ggcactacag cgtgtgataa ggcatcacca
     5281 atcaaagacc agccttttaa catcaaaaag gcggaaagaa aagcacaaac taaccccacg
     5341 cccatactga caaaaatcgc tttttgcata tagttgtagg taaaaggctc taataaaaga
     5401 tcaagcataa aatcctaaca agttgttgat aatagttcgc atatactatc atataaactc
     5461 cttttcttgt atggattaaa cttaaatatc tactttaaat tgataaggat caggatagta
     5521 ataacgaaaa ccaagctctt ggcaaatttt gtctgctttt atcagcctta caagcggttg
     5581 attttcttca gaaaaatgca aatccgctaa cccaaaacgt tttgccattt cactataata
     5641 atctttacgg cttgggtgct gttcggcaca taaatgaaaa atacgctgcc cgttcggctg
     5701 ctccaacagc aatgaaatgg ctgcaataca gtcctctaaa tgcactaaat tgaccggctg
     5761 ccctgctcca ctcaagttct gttttcccgc caaataaaaa accgggtgcc gttttttgcc
     5821 caccaagcca gctaaacgca aaatatcaca atgaattggc tggctcagca accaattttc
     5881 catttttact aataggttag cagaatcaac cactgcattt tcatcaaaaa cgcccgattt
     5941 cattggcaaa accgaagtcg agctggtgaa aaccagatgt ttcaagccct tttctattgc
     6001 aagcgaaact aatgtttgaa taccgcaacc gtaattttca gggcgaattt ggctaagggg
     6061 caaggcaatc actaacgcat cagcagcaag taattgctcg atttcgcttg tcactgctaa
     6121 cgcatttaaa tctagaggat aacattctat cgctaattct ccgaccgttc gcttagtacc
     6181 tgcaacacgc cagcccttat tttgtaatgc ttcagctaaa ggtagtccga gccagccaag
     6241 ccctaaaatg gtaatttttt tcatcattta cgtttctcaa gattaaaatt ggaatgaggg
     6301 gaaattcagg tacaaaaaaa gcacctcgtg gtgctatcta ttttatttct agattggagc
     6361 gggaaacgag gctcgaactc gcgaccccga ccttggcaag gtcgtgctct accaactgag
     6421 ctattcccgc aaattttaaa ttgttgattt tctaaatggt gcccgaggcc agacttgaac
     6481 tggcacgccc cgaaaggcga gggattttaa atcccttgcg tctaccgatt ccgccactcg
     6541 ggcagcacga tatagaaaaa ttggagcggg aaacgaggct cgaactcgcg accccgacct
     6601 tggcaaggtc gtgctctacc aactgagcta ttcccgcatt taaaaacgct acgaacctcg
     6661 tagagtctca acaggtgcat attctatgga tttcagattt aatgtcaaat aaatttgtag
     6721 gaaatctgta aaaaatcgtt taattggtga aaaataacgc tttttgcaaa tttttcatca
     6781 aatttgaccg cttgcagccg ccttatttgt tgaaatcata atcagcaaat ttccgacaaa
     6841 tatcaattac aacttttgtg gctttttcca tcgtatcaac cgaaataaac tcaaatcgtc
     6901 cgtggaaatt ttcgccgccg gtaaataaat tcgggcaagg caagcccata aatgaaaggc
     6961 gtgagccatc agtaccgcca cggatcggtt ctaaattcgg ctcaatgcct tgttcacgca
     7021 ttgcgttttc ggcaatttca accaagtagc cgtaatcgtc cagcacttca cgcatattgc
     7081 gatattgctc gacaatctcg acttttccgc aatgttcgcc gtaaatctgt tgcatctctg
     7141 ccagcgtatc attcagcatt ttgccgaatg cagctaattc atcacgctca aagctacgta
     7201 aaatatagtg aatttccgct ttatcaattc caccctcaat gccgtgcaac ataataaagc
     7261 cttcacgccc ttcgctggtg gcaggggtta aatggggagg cagacggtgt tggaactcgc
     7321 aagctcgctc taaggcgttg atcatcttgc cctttgccgt gccggggtga acgctacgcc
     7381 cgaagaacgt aacggtcgct gcatcggcat tgaaattttc atattgcaat tcgcccagag
     7441 gcccgccatc aaaggtaaag gcgacatcgg caccgaatgc gtttacatca aaatgatctg
     7501 cgcctcgacc gatctcttca tcggtggtaa agccgatgcg gatcttgccg tgcggaatat
     7561 gcggattggc ttgtaaatac tcgatagccg agagaataat cgcaatgccg gatttgtcgt
     7621 ctgcgccaag caaagaggtg ccgtcagtgg tgactaaggt gtcgccaatc agcttgttta
     7681 aattcgggaa atccgttggc gaaagataat cgccactgcc gtttagcaaa atattgccgc
     7741 cttgataagc ctcaatcacc tgtggtttca cgttttcacc attaaaatcg ggcgaagtat
     7801 caacgtgggc aatcagccca agcaccggca gggttttgtc aatattggca ggcaagctgg
     7861 cgaacagata gccgttgggg tcaagataaa tctcgcttag ccccatcgcc tctacttccg
     7921 ttttcagcag atgaagcaaa tcccactgct gttgcgagct tggcacagtt tggcttttcg
     7981 gatctgaagt tgtgtgaatt tgggtgtaac gtagtaaacg gttaagaagg gattgtttca
     8041 ttgtttttcc ttactatagt ttagtgtaac caattctcca atttcagatc aggtaccatt
     8101 ttaaatgcct gatcactaga aactaaaata cactcttcag caagagcgtg actagcaatc
     8161 atttgatcct gcacgcccat tactttgcca tttttctcca tctctgcccg taactttcca
     8221 taaagttcag cacaacgata attccaatct aaaaccttta cactcgataa aaaataatct
     8281 aaaaattgag aaagctgccc attatttctt ttcgccacac cataaaccaa ttcagcagca
     8341 gttacagatg aaatacaaag cctctcagga ttaatggact tcaatttcgc caccacattt
     8401 gcatttccac gaaaaaaata gcttacagta ttcgtatcta acatatacat ttaagctacc
     8461 tcaaaaggat cccgttgctc aaaggattgg tttctctctt ttgtatctaa aaatgtttca
     8521 tcacatacaa cagcaggcaa tagctcaaac aatcgttgcc aatgctcggt atttctcgat
     8581 ctcaatgata ataaaatatc gccattttct tttttagaaa catagacttg ctctgcatca
     8641 aactcaaatt caaccggtaa acgcactgct tggttccgcc cgttacggaa taatctcgca
     8701 attttttcca tcttacttta ccctccgata gatagtgtat atgccaaagt atatgcctat
     8761 tttatacaac tggcaagata acccattctc aaatactcaa taacatactg atttcaaatc
     8821 ataatattac caactaatag tcggtttacg tttctgctca acgcaatctt tcaaataaag
     8881 attgatcaac gtttggtaag gcaaattcac ctcttcggca agttgtttaa aatagctgat
     8941 aacatcgtct tcaatccgaa tagtaaccac acttttgctt tttagccctt ttgcgtaagg
     9001 gtttttcact gcattagaaa aatcgtattc atctctcatt taatgtactc ctttatattg
     9061 cccactttcg cgttttgttg ccaaacgtgc tgaaataatc cgaatgttgg tatttctctc
     9121 gcaatgcaca accacaagta gattatcttt attgctacgc ccgagtagca aaaaacgctc
     9181 ttccctagat gagtgatctg gatccgggat taacatcgca ttattatcat aaaatacgct
     9241 taccgcctcg ataaaactaa ctccgtgttt ttgttgatta cgaagagctt tctcactatc
     9301 ccattcaaaa ccattaattt cttccattgc cagtttactc ctttttaatt gtaaataaaa
     9361 tatatttaca tctatttttt taagttataa acagcctaga tgaaattact ggattcgcaa
     9421 taaacaaaaa cgatttttac gatacagatc gcaaaaatcg tttttcaaga ttgacaacct
     9481 taggaggctt tatccgccca caacctccct tagcaaattt tccacaaatt ctaaccgctt
     9541 gcggttgtct tcgagtgcta agcggaattt gaacttcacc gccccttcaa aactgtacac
     9601 cgctggttgt ttttggatta aggcgataaa cttcattggg tcgggctggg ctgtcggttt
     9661 aaattcaaga taaccgccgt gaatgccggc atcgattttc ttaatcccga gcgtttgtgc
     9721 cgacaagcga agttgggtta tcgcaaacag gtttttggtt gcctctggga gcaagccgaa
     9781 gcgatcgatc agctccactt tcaagtcgtt caggctttgc agattttccg agcttgcgat
     9841 ccgcttgtag aacgacagac gcatattcac gtcaggcaga taatcgtccg gcaataaggc
     9901 cggtacacgc aattcaatct ccgcttggtg ttgggtgatt tcatcgaggc tcggttctcg
     9961 cccttcttgc aaagctttga ccgcattttc gagcaagtcc atatagagcg aaaaaccgat
    10021 gctttcaatc tgtccgctct gctccgaacc gagcaattcg cctgcaccac gaatttctaa
    10081 atcgtgggtc gccaacgcaa aaccggcccc gagattatca atggtactca tcgcctccaa
    10141 gcgttgctcg gcatctttgg tgagcgtttt cgggtgcggt gtgagcatat aggcgtaggc
    10201 ttggtggtgc gagcgtccga cccgtccacg cagttggtgc agttgtgcca gcccgaattt
    10261 gtccgcccgt tcgataataa tggtattggc agtcggcacg tcaatccccg tttcaataat
    10321 ggtggagcaa accagcaggt taaagcgttg atgatagaaa tcgctcatca cccgctccaa
    10381 ttcacgctca cgcatctgcc cgtgtccgat cacaattctt gcctctggca cgagttcagc
    10441 caatttctcg gcacaatttt caatggttgc cacatcgtta tggaggtagt aaacctgccc
    10501 accacgcaga atttcacgca aaattgcctc acggattacc atttcatcac tttgtcgcac
    10561 aaaggttttg attaaaagtc ggcgagccgg cgggctggcg ataatcgaga gatcacgcat
    10621 tccgttgagt gccatattca gcgttcttgg aattggcgtg gcggtgaggg tcagaatatc
    10681 cacattggca cgcagttgtt tgattttctc tttttggcgt acgccgaaac ggtgttcttc
    10741 atcaatcact aaaagcccaa gatcacggaa cgctacatcg tcttgcaaca gtttgtgcgt
    10801 gccgaccaaa atatccactt tgccctccgc cacattttgc aaaatcgttt tctgctcttt
    10861 ggcggtttta aagcgagaca gcacttccac attcacaggg taattggcga aacggtcttt
    10921 gaaattctca taatgctgtt gggcgagcaa ggtagttggg gcaagaataa ccacctgttt
    10981 atggttcatc actgccaaga aagtcgctcg aatggccact tcggttttgc cgaagccgac
    11041 atcgccacac accaatctat ccatcgcttt ggcttggcac atatcgctaa ttacggcatt
    11101 gatggcaatt ttctgatcgt ccgtttcctc ataagggaaa gtggcactaa actgatcaaa
    11161 ttccgcttta tcgtaggcaa acgcaaaacc ggtttggctt tctcgtttgg cataaacatc
    11221 aagcaattct gccgccacat cacggatttt ttccgctgct ttttggcggg ttttcgccca
    11281 cgcttcactg cctaatttat gtagcggcgc agtttcatcc gccccaccga tataacggct
    11341 gattaaatgg agcgaggcaa ccggcacata gagtttggca tcgttagcat aatgtagcac
    11401 gagatattcg gctttgatcc caccagcgtc cagcacggtt aagccaccgt aacgccccac
    11461 gccgttttcc aaatgcacca ccgcctgtcc gattttgagt tctgctaaat tacggattaa
    11521 ggtatcagga ttgacggctt tgcggttttt ctcacgccgt gtttgcacac gttcgcccag
    11581 caggtcggtt tcgcagatga tggcaaggtt gcgggcgggc acaggacccg cccctacgta
    11641 gttattatca ttttcgccat ttgtatttaa ttcatcatcg tagggacggg tcccgtgccc
    11701 gcccgtttcc accacaaacc cttgatcaag cggtgaaata atcagggaaa atggcaaacc
    11761 ttgtgcttca tctaatgaat tgacctgctt cggcttcacc ccaagcggag caagcaaatc
    11821 cagcaaggtt tctcttcgcc cttcgctctc caccgaaaac agaatgttgc cgtcaaattt
    11881 ttggcggaat tgctggaaag cagaaaacgg ctctttggca gaggattgaa tagcaatatc
    11941 cggcaagtta gccacatttg cattcatttt cgctgctgat ttacggattt tctccgaagt
    12001 tatcgttaaa cggggatagc ctttcagcca gcgatttacc tcatcaatcg caaaccagag
    12061 atcactcggc ggcagtagcg gtcgcatcgg atccactcgg tggctttcat agcggttttg
    12121 ggtatcttgg tgaaattgtt cagctttttg ctgaatatcg gcgaaagtga taaacagcgt
    12181 ttgggcaggg aaatagtcaa acagacttgc catttcctca aaaaacaacg gctgccaata
    12241 ctcaatccct gcatttaaaa tgcctttgct gacttgctga taaatatgct ccggctcacg
    12301 gcggatttca ccgaattttt cacggaattt ggctctaaaa tgctcgatcc cgttgctatc
    12361 agtcgggaac tcgtgtgccg gcagtaaatt aatttcggca atttccgttt tggtgcgttg
    12421 gttatccaca tcaaaagtgc ggatactgtc gatctcatca tcgaaaaaat cgaggcgaaa
    12481 cggctcttct gctcccatcg ggaataggtc taaaatcgag ccacgcacgg cgtattcacc
    12541 atattccaac acctgttcca ccgcccgata gccggcattt tccagttgta accgcagttt
    12601 ttgaatcgaa aaacggtcgc cttttttaat caacagcaca ttgttagcga gataattcgg
    12661 cgggcagacc ttctgcatta aggtgttgat cggcaacagc aaaatctgct gtcgcccttg
    12721 ttgcagttca aacagggcag aaagtcgggc ggagataatg tcttggtgcg gcgaaaaatt
    12781 gtcataaggc aaggtttccc aatcggggaa cagttgcact ggcagccctg caaactgagg
    12841 caaggctttt tccaagcgta acgccgtgcg ggtgtcgggt gtgacaacca ccgtcaagcc
    12901 gtaaaaggct cgggcagctt ggctgatcgc aagggtatcg gaatgcccga caaggttacc
    12961 gagggtttga tgatcttgaa aattgccgtc agatttcggc agattaagtg aaattaaaga
    13021 catacaagcg gtctgtttta aataaaaaat tgcaaattgt tgcttagttt actaaagcac
    13081 ggctgattgt gcaaaataag cggagaaatt atgttatcta gatcgcattg cgaaaaaatt
    13141 ctgataaaat gttccccttt tttaactctt actcacacat attatgaaaa ataaaatttt
    13201 aggaagtgca ttaatgattg caggcactac cattggtgca ggaatgcttg caatgccact
    13261 cacctctgca ggaatgggtt ttggggcaac cgcattactc ttagtcggat tatgggcttt
    13321 attggcctac accggtttat tatttatgga agtttatcaa accgctccgc aaaaagatgt
    13381 tggagtggca agccttgccg agcaatactt tggcttaatc gggcgtgtat tagccacttt
    13441 cagtttgctc gtactacttt atgccctact tgcggcttat attaccggtg gtggttcact
    13501 gttagccggt gtaatgcctg aaatggcaga tgctgatatg aaactgaaaa tcagcatttt
    13561 aatcttcact gcggtattag gtacatttgt ggtagtcggc gtgaaatcgg ttgatggttt
    13621 aacccgagta ctgtttttgg gaaaaattgt tgcttttatt gccgtgttgc tgatgatgct
    13681 gcctaaagcg aagctggaaa acttgacagc aattccatta gataacttac tcattatttc
    13741 tgctataccc attttcttca ctgcgttcgg tttccacgtt attatgggaa caattaatag
    13801 ctatttagac gcagatatcg gcaaaattcg taaatctatt tatatcggga cggcaattcc
    13861 gctagtggct tatttattgt ggcagctcgc tacacacggg gtattaagcc aaaatgaatt
    13921 caccacaatt ttgacacaag atccgacctt aaacggcttg gtgaaagcca ctagccagat
    13981 taccggtagt acgattttag gcgaaatggt tcgcttattc tcagtgcttg cattgattac
    14041 ctcattttta ggggtttcga tgggaatttt tgaaggcgtg ggcgatttgt taaaacgctt
    14101 aaatctaccg actaaccgtt tatggctgac catcgccacc tttacaccgc caattctgtt
    14161 cgctttattc tatccgaacg gctttattaa ggcattaggt tacgccggtt tactctttgc
    14221 gttctatggt atgattttac ctatcggctt agcgtggaaa gcacgtcgcc aatatccgaa
    14281 tttaccttac cgtgtgattg gtggtaattt ggcattagtg gttgcgttaa ttgccggtat
    14341 tatcattatg gtcattccgt ttttaattca ggctggctat ttaccgcaag cggtaggcta
    14401 atattgaaaa gggctacgat tcaatatcgt agccctttct ataagttaca agcggtcatt
    14461 tttcgatgac tttttacaga ttagccaatc aactgataca gcgccttaat ttgatgctct
    14521 tttaatgccc cgccaatttt ctctctgccg acaaaaatct taaacattgc gttgcctttt
    14581 ttattaatga aaatcagtga aacagtacgt tttcccataa aaggacgatc caacaaataa
    14641 attttggcac aattttcgta gcgtagatgc ccttgtaagc ctccgccctc tttatgttcg
    14701 aaattgtaaa atccacgaga aattgaaccg cttgggagct ttcccacaaa ctcaataatg
    14761 gtatcttccg tatgcgaaat cagcgtaatc gcatcgtccc attcgtggat ttgctgtagc
    14821 acttcggcaa aacgttcccc ttcggttact tttagcattt cagccggtaa attcagcatt
    14881 acctcttcca gcgtgcattg atattttgcc gctaacattt ctaaaatttg ccccggattt
    14941 tcggctaatt cattgcgtaa ggcggtttgt tgttcgattg atatcattaa atgttccttt
    15001 aagtaaagag agtttcttag ctctctaagt aaatttgtaa attagaaacg gtattcaaaa
    15061 ttagccgaga agttacgtcc aggttcataa tagcgttgta atccaggata gccaccgtaa
    15121 gttgcatttt cacgtactcc aacagaatta acgattccac ctgttgtatt taaaccacgt
    15181 aaagtatccc aagtatggta tttacggtta aacaaattat aaataccggc acgtaatgta
    15241 atattctcag ttggtttata gaaaccgtat aaatccacaa caaaggcttt agcatttaag
    15301 tgcttccagg tatctaaacc tgtctcgtga gaatattcag tacaacgaat gtcatattca
    15361 tccccccaat aagggtaata cggatttggc gttctatcct ctttaaggca tttttccggt
    15421 aatgttttta aatattttgc atctttcgcc tttttagccc ccaagaatgt gaagcgagaa
    15481 aatactcccc atttaccttc aggttgttca taatctaagc caactacggc tttaatcggc
    15541 tgaatagaaa gtaaatctgc cccgttagac atactacctt tgctgtaacc gaaggctcca
    15601 aatagcttcc agccttcagg tattggcata gctgaattta aattccattt accactcact
    15661 tctagcccgt agattttcgc agaatcaata ttttgcattt gctgtaccgg gcgccaaaac
    15721 tctctaccat atgcttggta aacatcccag gtttcttgct cccataaaaa atctttatat
    15781 ttagtatggt ggaaattaac tgagagcagc ccaacattgc cactaccttg caaacttaaa
    15841 ttttggctta aactacgttc cgcttttagg tttggattgg acatccaagc tcctgcagca
    15901 ttatcacggt aatcaaaata catttctgat gcatttggaa tacggaagcc tgtcccaatg
    15961 ttatatccta ctttccatgc ctcagtcaga tgtttttcta aaccggtaac ccaagttagt
    16021 tgattaaatt tcgcattgtc agtttttacg cagttacgac aaacaagacc attgagttct
    16081 tgttgttcat acttcgccca atcataacga atgcctagcg ttgcaatcca atcatgcgtt
    16141 aaatcaatct tatcgtgcaa tgaaaaagtg tgctgtttcg ttttaattgg gtacatcatc
    16201 gtagaatcgg gagaatttac ccaaacaccg gataaataaa ccgagtcctt atgtaataca
    16261 tcgaaatctc tctctgcagt agaggctttg aaagatagct gatgagtcca tttaccaaat
    16321 tctaatgctt ggctgtctaa acggaaattt aagcgtttca attcagtatt aaaaatacga
    16381 atattattgt cacttggttt tctttgttta gcagggctcc agtctgttgc atcttcagcg
    16441 cgtgtacctt catagttata agcagatgtt tcagtatctt gataatctgc atctatcttc
    16501 gctagagata tccattttga ctctgggaaa tactcataaa aaacatttac tgtatcacgt
    16561 ttactacggt cttccgcttc acgccaaaaa gaagtaatag taacggctga atcttcgata
    16621 atataatttt tattattttg cccactgtaa gaaacaccaa cgcggtgatt atcattaaat
    16681 cgataagcaa atttggctaa ataattatga tgattatgag tagaatcatc aggagtttgt
    16741 ttactactgc gtaaagagcg agtttgtaga gaatcttcag gaagtgtata acctcctgca
    16801 cttttcattt catgcccatg gcgatttgaa taaaccaata tcgcctcagc tttctcacct
    16861 aaatatccaa caccgaatgt attcaaccat tcattattac gagtaccata tcctgtgcga
    16921 taaaaagcac caaatttatt accgttacga acaatatcat aaggctcaag cgtccgataa
    16981 ttaactccgc cgcctaaatt accactacct tgattgaaag aatctgcacc tttagatact
    17041 tcaatagtac gagctaattc cggatctata gattggcgag atgtatttaa attaccatag
    17101 cgtttataaa gggaattttc ttctgaatca ggaaaagcga gacaccatca atacttattc
    17161 ctacacggtt gtcttccact ccacgaatag caaatccttt ttgatgtcta ccttggttaa
    17221 caatgccaac atcagggcta taacgaacta aatctcgatt attttgaatc aactcttgct
    17281 gaattatctc tttatttttc ttttctatag aagccttcac atcttcttta gcaacaacat
    17341 ttacttcaga taattccgct gttgtttctt ccgcataggt agcttgtgaa gacaaaatgc
    17401 tactaacgct taaagccaat atagtcgggc acaatttttt agactttatt gaataagagg
    17461 taaacaccat gattctc
//
LOCUS       DANLRK010000034        17325 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.034, whole genome shotgun sequence.
ACCESSION   DANLRK010000034 DANLRK010000000
VERSION     DANLRK010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 17325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17325
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.034"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(47..649)
                     /locus_tag="PXA33_000827"
     CDS             complement(47..649)
                     /locus_tag="PXA33_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDL5120174.1"
                     /translation="METLIEFFSSYGYWAVFLVLLACGFGVPIPEDITLVSGGVISGL
                     GYTNVHWMLVVSMLGVLVGDSTMYWLGRIYGEKILKFPLIRNVATPERFATVQERFEK
                     QGWKLLFMARFLPGLRAVVYLVSGITRKVSFVKFLLVDFCAAIISVPIWVYLGDYGAS
                     NLDWLKEQVANGQHLIFAILGIVILFLGWKWYKNKKQYKK"
     gene            complement(651..1670)
                     /gene="purR"
                     /locus_tag="PXA33_000828"
     CDS             complement(651..1670)
                     /gene="purR"
                     /locus_tag="PXA33_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HDL5120175.1"
                     /translation="MATIKDVAKYAGVSTTTVSHVINKTRFVAEDTTKAVWEAIKALN
                     YSPSAVARSLKINTTKSIGMIITTSESPFFAEIVLAVEEFCYSKGYSLFLCNTQNKLE
                     KIQNHLDMLIKKRVDGILVMCSEYTANSFDIFENSNIPMVIMDWGRSDERSDIILDHG
                     VEGGYIATQHLIENGHQDIAVITGNRDKEIARTRFEGFKKALSEAGLSIREEWIQEGD
                     FEPEGGYECMNNLLKSSEKLPTAVFCFNDSMALGAISAITEKGLNVPQDISVIGYDNV
                     HSSRFYAPPLTTIHQSKSRLGSAALELLLERIEDNENAKEPKVLEFFPELVKRKSVRN
                     LNIKE"
     gene            complement(1806..2534)
                     /gene="cmoA"
                     /locus_tag="PXA33_000829"
     CDS             complement(1806..2534)
                     /gene="cmoA"
                     /locus_tag="PXA33_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HDL5120176.1"
                     /translation="MQRDTLFSAPIEKLGDFTFDESVAEVFPDMIQRSVPGYSNIITA
                     IGMLAERFVTDNSNVYDLGCSRGAGILSIRRNVNAKNVQIIGVDNSQPMVERCKTHLS
                     AYHSEIPVTILLDDIRHIEIKNASMVVLNFTLQFLPREDRLALLQKIYQGLNSNGILV
                     LSEKFTFESEEMNELLIDLHHTFKRANGYSELEVSQKRNALENVMRTDSIETHKKRLQ
                     EAGFTQVELWFQCFNFGSMIAIKA"
     gene            complement(2544..3326)
                     /locus_tag="PXA33_000830"
     CDS             complement(2544..3326)
                     /locus_tag="PXA33_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDL5120177.1"
                     /translation="MTEKSQLNFNYQPSNNHANAQTMIFLHGLFGDLNNLGAIAKPFA
                     ERYSILMVDLRNHGRSFHSDEMNYALIAQDLAQLLTELNIQNAIVVGHSMGGKSAMAL
                     AAFAPELVEKLVVIDIAPVTYTQNRHQQVMAALFAVKEAKPQNRQQAKAIMAEYIRDE
                     GVQQFMLKSFDAQSEQSFRFNLQALKDNYQNVMGWQQVFVDKPTLFIKGALSDYIQAE
                     HTQTILAQFPQAKSFIVANADHWVHAEKTGTVVRAINKFLEK"
     gene            3445..4011
                     /gene="seqA"
                     /locus_tag="PXA33_000831"
     CDS             3445..4011
                     /gene="seqA"
                     /locus_tag="PXA33_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HDL5120178.1"
                     /translation="MKRIEIDDELYQYIASRTQSIGETASDILRRLLRLPQSPQPFVL
                     VQEHMINELKELVKTPSRATKKNESNAEKTVEKLENILNSEHFMNENKNVVRFTMLLA
                     ALYRANPEAFAKATEKVRGNERIYFSQSEAEILATGSGVKAKQIPDSPFWVITNNNTA
                     RKGLILKGVMESMKVPSQLIERIQALFI"
     gene            4021..5439
                     /gene="menE"
                     /locus_tag="PXA33_000832"
     CDS             4021..5439
                     /gene="menE"
                     /locus_tag="PXA33_000832"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HDL5120179.1"
                     /translation="MLINSTRFPTQFWAAHSAQNVAIIWEKGTSDYLNHLPSNITWYS
                     LNQLIAQAIFVLQQQGATPHSLIAYCGSHRLIGLLTYCASLALGAKILMLNPAQTASQ
                     RQAVLDDNGVELIINDADFAKFSAKTTACNAFPTIDFEKPATLTLTSGSSGKPKAVVH
                     SIAAHLYSAEGVCELMAFEQPNSWLLSLPLFHVSGQGIIWRWLFKGATLYVHEDKSDF
                     FLSAQKVSHLSLVPTQLQRYLNQLDSMVSQKCLLGGSMLPAELIAQAQQSGMTTFSGY
                     GMTEMASTICAVENDLNSVGFPLKHRELKLENGEIWVKGKSLGLGYWQKNGQIRPLVN
                     TQGWFATKDRGEWNHKKQLVIKGRLDNMFISGGENIQPEEIEQLIFRSQLVEQIIVLP
                     IEDKQFGHRPVAFLQFKQSDSKNELHKLKIWLSDKLERFKQPVAYYLLDIEKYQKQGL
                     IKLSRTQLQQDLKLKVLKEIYV"
     gene            5432..8809
                     /gene="mscK"
                     /locus_tag="PXA33_000833"
     CDS             5432..8809
                     /gene="mscK"
                     /locus_tag="PXA33_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HDL5120180.1"
                     /translation="MFRPILKTFVLSLGLMLNSYALAELPTQQVVQTQLDSAKKLDQA
                     DADNKALVQTLEDTLAFLAQIDKQKADNQALNKSIQDSQKALKTSQHNLEKLKEVTIA
                     SVDNLTQKATTDLQKELTSTQSQLETVQHDLSIINNNLVAQNTAPDKAQTALTEMVKR
                     KQEISSQLSATNIKNELQTKLEVELELIELKNTYNQILLGGSEDLSALYSAQLDEKKQ
                     EQQNLQAEIANLQTAINNKIVEESQNKLDQAQAVQNQQNNATTNPVILRELDINTKVT
                     QELLKQTKDMTQLSQDNLRIKSVLDNLQQTQRNIEEQISSLQGTLVLSRIINKQKQSL
                     PQDEMISGLSKQIADLRVRVFDITEFKDSFADINAYISRIEQDEKTTFTSKEKEQLSK
                     ILQERSDTLTEMIKSLNNQLNLLINIELNQQQVQTISDALQQKLQQQSFWVKSNNPID
                     LEWFSKFVDITHYQFIDIGKKLNFSNWRDNLLPASILVGILLLLAIFINRQKDKIKQK
                     LHQINNRLKIVATDSQWNTPLAIFWTLVLCLPSTLMFLAGFVAITYISFENPQEVWTW
                     GLKMAIYWLYFAFLVAMLRPHGIGHIHFNMPQKSNEIFRNILKRSVWIIALLLNTSIF
                     TNLEMGISYDMLGQTMTIFVLILMIWMIVPAFRQAISTYENVTTEEKKESKSILLTIV
                     KLVLLLAPIILIILIALGYYYTALVIIEHLVDSYFAIITWIIIRNILYRAFSISSRRL
                     AYRRLQEKREAAEAKTRAKAEQNDENPDGVELPFELKEDSIAVSEVKNQMLKLTDFVL
                     WIGLFVLLYWVWSDLITVALYLDGVTLWQQSVTTEAGIVMESITLLNLLTAILIIVVM
                     VVLIRNLSGLLEALIFSHFKLSQGTPYTITTLLTYLIIALGAIFAFGTLGMSWSKLQW
                     LLTALSVGLGFGMQEIFANFVSGIIILFERPMRIGDTITIGTYSGTVSKIRIRATTLV
                     DFDGKEVIVPNKAFVTERLVNWALSSTATRIIVQVGVAYGSDLELVKRLLLQVAEENP
                     KVLKDPQPRAYFLTFGASTLDHELRAYVNDLSDRNPTIDALNTRIHQLFNEHNIDIAF
                     NQLDVYIKNNDTQDEIKVDSKRFEFERPNNITAKEKE"
     gene            8812..9894
                     /gene="aroC"
                     /locus_tag="PXA33_000834"
     CDS             8812..9894
                     /gene="aroC"
                     /locus_tag="PXA33_000834"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012072711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HDL5120181.1"
                     /translation="MAGNSIGQLFKVTTFGESHGIALGCIVDGVPPNMALSEADLQPD
                     LDRRKPGTSRYTTPRREDDEVQILSGVFEGKTTGTSIGLIIKNGDQRSQDYGDIAEKF
                     RPGHADYTYQQKYGIRDYRGGGRSSARETAMRVAAGAIAKKYLREQFGIEVRGYLSQI
                     GNIKIDPKTVENIEQIDWHAVNSNPFFCPDPVAIEGFDALIRELKKEGDSVGAKLTVI
                     AERVPVGLGEPVFDRLDADLAHALMSINAVKAVEIGDGFAVVEQRGSQHRDEMTPQGF
                     LSNHAGGILGGISSGQPIIAHIALKPTSSITIPGRSVNKNNEAVEVITKGRHDPCVGI
                     RAVPIAEAMMAIVLLDHLLRFKAQCR"
     gene            9913..10740
                     /gene="mepA"
                     /locus_tag="PXA33_000835"
     CDS             9913..10740
                     /gene="mepA"
                     /locus_tag="PXA33_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HDL5120182.1"
                     /translation="MKLLKTLLVSSLAFTATALNATQWEKIKTPVQGKAQSIGGYSNG
                     CIIGAQPLALKGEGYQVIRSARNRYYGHPQLLDYLTHLGKKTKAAGVPTILIGDMGMP
                     AGGRFASGHASHQTGLDADIWFKFGPMDDETARNPAGLATIMVNRSTERVDDSLWNQH
                     HTNLVKLAATDPRVDRIFVNPAIKVKLCQTAGNDRAWLRKIRPWYGHDSHFHVRLTCP
                     ADAASCENQALVPAGDGCGEELYSWFEPKEASGTPKAKVLPTAPVQCQMILSEQGLN"
     gene            10813..12732
                     /locus_tag="PXA33_000836"
     CDS             10813..12732
                     /locus_tag="PXA33_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDL5120183.1"
                     /translation="MRLFVAEKPSLARAIADVLPKPHTKGDGFIQCGEHDTVTWCIGH
                     LLEQAEPDVYDERFKMWRMEHLPIVPEQWKLIPKKETLKQFKVVEKLIKKADVLVNAG
                     DPDREGQLLVDEVFGYLNLSAERKSQIQRCLVSDLNPNAVSRAIEKLQKNSDFIPLAT
                     SALARARADWLYGINMTRAYTLQGRWAGYKGVLSVGRVQTPVLGLIVRRDLEIEHFVP
                     KDYFEVLAQIVVPETQERFKAQWQPSKACEDYQDEDGRVLSRPLAENVVKRITDQPAT
                     VTEYQNKIEKETAPLPYSLSVLQMEAAGRYGLSAQAVLDICQKLYETHKLITYPRSDN
                     RYLPTEHFNDRFKVMAAIAHHLPEYQQKPEVIDATIRNRCWNDKEVEAHHAIIPTARQ
                     GTVHLTENEKRVYQLIARQYLMQFCPDAEYRKSKIGLEIAGGSFVAQARNLVIAGWRA
                     LLGKEDSLDDAEPLLPIVKKGQILHCEKGEIVSKKTQPPRHFTDRSLLSAMTGIARFV
                     QDKELKKILRETDGLGTEATRAGIIELLFKRGFLIRKGKNIHSTEAGRILIQALPDSA
                     TQPDMTAHWEMQLNQISKKETSYQQFMFDLNQQLPHLLQSPNRQVLQNLAKITPLASG
                     NKPRYFKKRTQTAEN"
     gene            12838..13164
                     /gene="secG"
                     /locus_tag="PXA33_000837"
     CDS             12838..13164
                     /gene="secG"
                     /locus_tag="PXA33_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HDL5120184.1"
                     /translation="MLINVLTIVYLVVAVLLIAFVLMQQGKGADAGASFGAGASATVF
                     GSAGSANFLSRTTALLATIFFAISLVIGNLSSHNVAPKSQFEDLSQVEQKQVEPVKIE
                     NSDIPQ"
     gene            13189..13274
                     /locus_tag="PXA33_000838"
     tRNA            13189..13274
                     /locus_tag="PXA33_000838"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13223..13225,aa:Leu,seq:gag)
     gene            13477..14718
                     /locus_tag="PXA33_000839"
     CDS             13477..14718
                     /locus_tag="PXA33_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL5120185.1"
                     /translation="MARTTKALNKTQIENAKSKDKEYILSDGDGLNLRVKPLPKGSKI
                     WIFNYTNPQTKNRTNLTIGRYPLITLAEARTICDGYRSLIEKGIDPQEEKKRLQQEAE
                     NRVNDTFRNVAESYFNGIYKEKAKNPITREKNWIRLENHIFPYIGDKHVEEIKVKALV
                     DIYSKVADKSNTLKKLHQLVSAVMDHAITQGIIESHNCRLAVKNFYIKPSTPNPTIEL
                     EELPQLFTDLHNAKLSKQTYALVCWSFLTALRPNEAVNAEWKEIDFEKKLWNIPKEKM
                     KGKADKKRPHSVPLSSQALKILEMMKMHSNSSPFVFPGRSSNSKPMSNATVNSTLKRN
                     GYKDRLTAHGIRAFVKTFLSSRKVDRHVSESILSHLLEGGDDLENTYNRYDFLEERVP
                     VMQLLGDYCEQCGMNLELVRG"
     gene            complement(14877..16745)
                     /locus_tag="PXA33_000840"
     CDS             complement(14877..16745)
                     /locus_tag="PXA33_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDL5120186.1"
                     /translation="MFKKLLELRQQKAAIEAEMRSILTKADEEKRSTTEAEGAKFDEL
                     RSQAENLVKEIARYEAITDEQRNQPGQQVETANVKVSDDELRHWIKTGELRSLATNTN
                     GGADGGYSVIPELDKQVMKRLTDDSVMRQIANVVKLTQGKEYKKLVSAGGAAVNHIGE
                     GEARTETATPKMNEVTIAVHNIYAYPKTTQEILDFGGVDILGWLTEEISESFVETEET
                     DLTNGTGTKQAKGFLAYPRTTANDKTRPFGTLQKMEVQPATVIGWNSKGQPETLTPYP
                     PYSVNVQRTVNRQNWLTAYIPHSYDENVAETPHFDQWLNFVSDGKQDKAKNILAALYA
                     ILTNRYNWQVFFQVTGKGGSGKSVFAGIATLLAGEKNTASARLENFDDERKIAGLEDK
                     KLIICSEQTKYAGDGGGLKAITGGDMVRVDPKNKHPFNARIAAMIMIVNNDPCKWTER
                     NGGIDRRIVNFRFNKRPPENERDPYFMDKITLEIGGIIRKVLDTFPDPLEAKRALEEQ
                     KESLEALEIKKQSDPLTDFFEYLYTTENTDGLYIGTGNTLGHDKIRTHLYPAYLAFVK
                     AKNVLELGLNTFVVGIEQAVKQHGNKHDFTKRKTNKGQRTNVHFKNFDDFQSDILN"
     gene            complement(16756..17151)
                     /locus_tag="PXA33_000841"
     CDS             complement(16756..17151)
                     /locus_tag="PXA33_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL5120187.1"
                     /translation="MISNEAIRQEVIDLLQGNIQGIQHFHNGRAVFTDVENELPAIAV
                     FIDEAEYSQLTLCDGECNAYLKIGIYLPMFSTENDLDLVAQKISDVMNQATFHSMDEC
                     VLAKYSYDYDANESAWKTATLHYNINYLN"
BASE COUNT         5363 a         3600 c         3366 g         4996 t
ORIGIN      
        1 tgttttgcta ttatatcttt ttaagatgaa atgctcgtct tttgtttcac tttttatact
       61 gctttttatt tttataccat ttccagccta aaaatagaat aacaatgcct aaaatggcaa
      121 aaatgaggtg ctgcccatta gctacttgtt cttttagcca atccaaatta gatgccccat
      181 agtcgcctaa ataaacccaa attggcacag agataatagc ggcacagaaa tcaactaata
      241 aaaattttac gaagctgact tttctggtaa tacctgaaac taagtaaacc accgcgcgca
      301 agccaggtaa aaaacgagcc ataaaaagta gtttccatcc ttgtttttca aaacgttctt
      361 gcacggttgc aaaacgttca ggtgtcgcaa cattcctgat taatggaaat ttgagaattt
      421 tttcgccata gattctacct agccagtaca ttgtgctatc gccaactaat actcctaaca
      481 tactaacaac cagcatccaa tgcacattgg tgtagcctaa tcctgaaata acaccaccgg
      541 atactaacgt aatatcttca ggaatcggca caccgaaacc acaggcaagt agcacaagaa
      601 aaacagccca ataaccatag ctactaaaaa attcaatcaa tgtttccata ttactcctta
      661 atgtttaaat tccgaaccga tttacgtttg accaattcag gaaagaactc taatacttta
      721 ggctcttttg cgttttcgtt atcttcaata cgttctaata ataattctaa tgctgccgag
      781 cctaagcgag attttgactg atggattgtc gtaagaggcg gtgcataaaa gcgtgagcta
      841 tgcacgttat catacccgat cactgaaata tcttgcggca cattcagccc tttttcggta
      901 atagctgaaa ttgcccctag tgccattgag tcattaaaac aaaaaaccgc ggttggtaat
      961 ttttcactgc tcttaagcaa attattcata cactcatacc ccccctcagg ttcaaaatcg
     1021 ccttcttgaa tccattcttc acgaatagac aaacccgcct cagatagcgc ttttttaaaa
     1081 ccttcaaaac gggttcttgc aatttcttta tctctattgc cggtaattac agcaatatct
     1141 tgatgcccgt tttcaattaa atgctgagtc gcaatatagc caccttcaac accgtgatct
     1201 aaaataatat cactgcgttc atcacttcgt ccccaatcca taatcaccat tgggatattg
     1261 ctgttttcaa agatatcaaa tgaattagct gtatattcgg agcacatcac caaaatgcca
     1321 tcaacccgtt ttttgatgag catatctaaa tgattttgaa ttttctcaag tttgttttga
     1381 gtattgcaca aaaatagtga ataccctttg ctatagcaaa attcctctac cgctaacaca
     1441 atttcagcga aaaacggtga ttcgctggtt gtaatgatca taccgataga tttagtcgta
     1501 ttaattttca aactccgcgc tacagcacta ggagaatagt tcagcgcttt aatagcctcc
     1561 caaactgctt ttgtagtgtc ctctgctaca aagcgggttt tgtttataac gtgagaaacc
     1621 gttgtagtcg atacaccagc atattttgcg acatctttta tcgttgccat acctttttcc
     1681 tcctaaataa gtagtggtgc attataactg attctaactg acttttatta taaagaaata
     1741 gatagaatgc aagcggttgc tttttatcaa gaatttgcaa aaaattttac aaatatcacc
     1801 gcttgttagg ctttaattgc aatcattgag ccaaaattaa agcattgaaa ccaaagttca
     1861 acttgtgtaa aaccagcttc ttgcagacgt tttttatggg tttcaatact atctgttcgc
     1921 atcacatttt ccaaagcatt acgcttttgg ctgacttcca attcactgta gccatttgct
     1981 cgtttgaatg tgtgatgaag gtcgatcaat agctcgttca tttcctcact ctcaaaggta
     2041 aatttttccg ataacactaa aatgccattg gaatttaatc cttgataaat cttctgtaat
     2101 aatgctaggc gatcttctct tggtaaaaat tgcaaggtga aattaagtac caccattgaa
     2161 gcatttttaa tttcaatgtg gcgaatatca tcaagtaaaa tcgtcaccgg gatctcagag
     2221 tgataagcac ttaaatgggt tttacaacgc tcaaccatcg gctgagaatt atctaccccg
     2281 ataatctgca cattcttcgc attcacatta cggcgaatag ataaaatccc tgcgcctcgt
     2341 gaacaaccta aatcataaac atttgaatta tctgtgacaa aacgctccgc caacattcca
     2401 atagcagtaa taatattgga atagcccggc acggagcgtt ggatcatatc cggaaacact
     2461 tcagcaaccg attcatcaaa agtaaaatcg cccaatttct caatcggagc ggaaaataat
     2521 gtatctcttt gcatttttct cccttatttt tctaaaaatt tattgattgc tctcacaaca
     2581 gttcctgttt tttctgcgtg aacccaatga tcagcgtttg ccacaatgaa agatttggcc
     2641 tgcggaaatt gtgccaaaat cgtttgtgta tgctctgctt gaatataatc cgataatgca
     2701 cctttaataa acaaggtcgg tttatccaca aatacctgct gccaccccat cacattctga
     2761 taattatctt ttagcgcctg taaattaaaa cggaaagatt gctcgctttg ggcatcaaaa
     2821 gatttcaaca taaactgttg cacgccttca tctcgaatat attcagccat tatcgcttta
     2881 gcttgctgac gattctgggg tttagcttct ttcaccgcaa ataaggctgc cataacctgc
     2941 tgatgccgat tttgggtata agtgaccggg gcaatatcaa tcactaccaa tttctccacc
     3001 agttcaggag caaatgcagc caatgccatc gcactttttc cgcccattga atgcccaacc
     3061 acaatagcat tttggatatt taattctgtg agtaattgtg ctaaatcttg tgcaatcaaa
     3121 gcataattca tttcgtctga atgaaaagat cgcccgtgat ttctgagatc aaccattaaa
     3181 atggagtaac gttctgcgaa tggttttgct attgcgccta ggttatttaa atctccaaac
     3241 aatccatgta agaagatcat cgtctgagca ttagcatgat tattcgaagg ttggtaatta
     3301 aagtttaact gcgatttttc tgtcatataa atgtcattgt tcatataaat tttcagcgat
     3361 gttcctctat aaaaaacaat agaaatgcgt ataatagcaa gcactattaa cgcatttaat
     3421 acccgcagat atttgaggat cccgatgaaa cgaatcgaaa ttgatgatga attatatcag
     3481 tatattgcaa gccgtacaca atctattggt gaaactgcgt cagatatatt acgccgtcta
     3541 ttacgcctgc cacaatcgcc tcagcctttt gttttagtac aagaacacat gatcaacgag
     3601 cttaaagagc ttgtcaaaac gccttctcgt gctacgaaaa aaaatgaatc caatgcagaa
     3661 aaaaccgttg aaaaattgga aaacatttta aattccgagc attttatgaa cgaaaataaa
     3721 aatgtagtgc gttttactat gctattagcc gcattatatc gagcaaatcc ggaggctttt
     3781 gctaaagcaa ccgaaaaagt gcgtggtaat gaacgtatct acttttcgca aagtgaagcc
     3841 gaaattttag ctactgggag tggagtaaaa gccaagcaga tcccagattc accgttttgg
     3901 gtaattacca ataataatac cgcacgcaag ggacttattc tcaaaggcgt aatggaatcc
     3961 atgaaagtac caagccagtt aatcgaacgc attcaagcgc tttttatcta attaattgaa
     4021 atgctcatca attctactcg ctttcctacc caattttggg ctgctcattc agcccaaaat
     4081 gtggcgatta tttgggaaaa aggaacatca gactatctta accaccttcc atcaaacatc
     4141 acttggtata gtttgaatca gttgattgcg caagctattt tcgtgttgca acaacaaggt
     4201 gctactccgc acagtttgat tgcctattgt ggttctcatc ggctcattgg cttgctcact
     4261 tattgtgcta gccttgcttt gggggctaag attctaatgc ttaaccctgc tcaaacagca
     4321 agtcaacgac aagcagtctt agatgacaat ggtgttgagc tcattattaa tgatgccgac
     4381 tttgcaaaat tttcagcaaa aacgaccgct tgtaatgcct tccctacgat tgattttgaa
     4441 aaaccagcta ccctaaccct aacctcaggc tcaagcggta aacctaaagc agtggtgcat
     4501 tctatagcgg cccatctcta tagtgccgaa ggagtatgtg aattaatggc atttgagcag
     4561 ccaaactctt ggctactgag cttgcctctg tttcacgttt ccggacaagg cattatttgg
     4621 cgttggttat ttaaaggtgc aacactgtat gttcacgaag acaaaagtga tttttttctt
     4681 tcagcacaaa aagtgagcca tctctcttta gtccccaccc aattacaacg ctatttaaac
     4741 caattagatt ctatggtcag ccaaaaatgt ttactgggtg gatctatgct tccagccgaa
     4801 ttgattgctc aagcacaaca atctgggatg actacctttt caggctacgg aatgactgag
     4861 atggcatcaa ccatttgtgc agtagaaaat gatttaaaca gtgttggttt tccgttaaaa
     4921 catcgggaac taaaattaga aaatggtgaa atttgggtaa aaggcaaatc actcggctta
     4981 ggctattggc aaaaaaatgg gcaaattcga ccgcttgtaa acacacaagg ctggtttgcc
     5041 actaaagatc gtggcgaatg gaatcacaaa aaacagcttg taataaaagg acgattggat
     5101 aatatgttta tttcaggagg ggaaaatatc caacctgagg aaattgaaca actgattttt
     5161 cgttctcagc ttgtcgaaca gattatcgtg ttgccgattg aagataagca atttggacat
     5221 cgaccagtgg cgttcttaca attcaagcaa tctgattcaa aaaatgaact ccataaatta
     5281 aaaatttggc tgtctgataa attagaaaga ttcaaacagc ctgttgccta ttatttactg
     5341 gatatagaaa aataccaaaa acaaggctta attaaactat caagaacaca attacaacaa
     5401 gatttaaaac tcaaagtatt aaaggaaatc tatgtttaga ccgattttaa aaacatttgt
     5461 gcttagttta ggcttaatgc taaatagtta tgcattagct gaattgccaa cccaacaggt
     5521 agtgcaaacg cagttagata gcgccaaaaa attagatcaa gccgatgcgg ataataaggc
     5581 attggttcaa acacttgaag atacgcttgc tttcttagcc caaattgata aacaaaaagc
     5641 agataatcag gcattgaata aatctattca agattcccaa aaagcattaa aaacaagcca
     5701 acataatctt gaaaaattaa aagaggtgac tattgcaagt gtcgataatt taacacaaaa
     5761 agcgacaaca gatttacaaa aagagttaac ctctacacaa tctcagctgg aaacagtaca
     5821 acacgattta agtattatta ataataattt agttgcacaa aataccgcac cggataaggc
     5881 tcaaacggcc ttaaccgaga tggttaaacg taaacaggaa attagtagcc aactttcagc
     5941 gactaacatt aaaaatgaac tacagacaaa gttagaagta gaattagaat taatagaatt
     6001 aaaaaacact tacaatcaaa tattacttgg tggcagcgag gatttatctg cgctttactc
     6061 tgctcaatta gatgaaaaaa agcaagagca gcaaaatcta caagcagaaa ttgcaaacct
     6121 gcagactgct atcaacaata aaattgtaga agagtctcag aacaagctag atcaagcaca
     6181 agcggttcaa aatcagcaaa ataatgcaac gacgaaccca gttatcttgc gtgaattgga
     6241 tataaatacc aaagtcacac aagagttgct aaaacagacc aaagatatga cgcagttatc
     6301 acaagataat ttgcgaatca aaagtgtgct agataactta cagcaaaccc aacgtaatat
     6361 tgaagaacaa atcagttctc tgcaaggtac gttggtgctt tctcgtatta ttaataagca
     6421 aaaacaatca ttaccacaag atgaaatgat ttccggacta tcaaaacaaa ttgcagattt
     6481 acgtgtacgt gtttttgata tcactgaatt taaagactct tttgcggaca taaatgctta
     6541 tatcagccgc atagagcaag atgaaaaaac aacctttacg agtaaagaga aagaacaact
     6601 gagtaaaatt ttacaggaac gttctgacac tttaactgag atgattaaat cattaaataa
     6661 tcagctcaat ttattaatca atattgaact aaaccagcaa caagtgcaga ccattagtga
     6721 tgcgttacag caaaaactgc aacagcaaag cttctgggtt aaaagtaaca accccattga
     6781 tcttgaatgg tttagtaaat ttgtcgatat tacccactat caatttattg atattggaaa
     6841 aaaactcaat ttttcaaatt ggcgggataa tttattacct gcaagtattt tagtcggaat
     6901 tttattgcta ttagccatct ttattaatcg ccaaaaagat aaaattaaac aaaaattaca
     6961 ccaaattaat aatcgactaa aaattgtcgc cacagatagc caatggaaca cccctcttgc
     7021 tattttttgg acattagtgc tttgcctgcc aagtacacta atgttcttag ccggatttgt
     7081 tgctatcact tatatttcat ttgaaaatcc tcaggaagtc tggacttggg gattaaaaat
     7141 ggcaatttat tggctgtatt ttgccttttt agtggcgatg ctacgcccgc acggcatagg
     7201 acatattcac tttaatatgc cacaaaaaag taatgaaatc ttccgtaata ttttaaaacg
     7261 ttcggtatgg atcattgctt tattactgaa cacctctatt tttacaaact tagagatggg
     7321 aatatcttac gacatgcttg gacaaacaat gactattttt gtccttatat taatgatttg
     7381 gatgattgta ccggcattta ggcaagctat ttctacctat gagaatgtca caacagaaga
     7441 gaaaaaagaa tctaaaagta ttttactcac tattgtcaaa ttagtgctac tgcttgcccc
     7501 tatcattctt atcattctga ttgcattggg ctattactat actgcactgg tgatcattga
     7561 gcatttagta gattcttatt ttgcgatcat tacttggatt attattcgta atattctcta
     7621 tcgagcattc tctatttcat cacgccgtct ggcttacaga cgtttacaag aaaaaagaga
     7681 agcagctgaa gccaaaacac gagctaaagc agaacaaaat gatgaaaacc cggacggtgt
     7741 cgagcttcct tttgagctaa aagaagactc tattgccgta agcgaagtca agaaccaaat
     7801 gttgaaatta actgattttg tgctttggat tggcttattt gtgctgcttt actgggtttg
     7861 gtcggactta attactgttg cactctacct agacggcgtt accttatggc aacaaagtgt
     7921 tacaacagaa gccgggattg taatggaatc cattaccctg ctgaatttac tcactgctat
     7981 tctgattatt gtggtaatgg tggtactcat tcggaactta tccggtttac ttgaagccct
     8041 gattttctcg cacttcaagt tatcgcaagg tacaccatac acaattacca cactcttaac
     8101 ctatttgatt attgcgttag gggcaatctt tgcgttcggt acattaggga tgtcttggag
     8161 caagctccaa tggctactaa ctgcactttc tgtcggtctg ggttttggta tgcaagagat
     8221 ctttgcaaac ttcgtttcag ggattatcat cttgtttgaa cgtccaatgc ggattggcga
     8281 caccattacc atcggcactt acagcggtac ggtttccaaa attcgtattc gtgccacaac
     8341 cttagtcgat tttgacggca aagaagtgat tgtaccaaac aaagcattcg taaccgaacg
     8401 cttggtgaac tgggcattaa gcagcaccgc aacccgcatt attgttcaag tcggcgtggc
     8461 ttacgggtct gatttagaat tagtcaaacg gctgttatta caagtggcgg aagaaaatcc
     8521 gaaagtcttg aaagatccac aaccaagggc ttatttcctt acctttggtg caagcacttt
     8581 agatcacgaa ttaagagcct atgtgaacga tttatctgat cgtaacccaa ctattgatgc
     8641 actaaataca cgcatccacc agctctttaa tgaacataat attgatattg catttaacca
     8701 attagatgtt tatatcaaaa acaacgatac acaagatgaa attaaagtag attcaaaacg
     8761 ctttgagttt gaacgaccaa ataacataac agcaaaagaa aaggaataac aatggcaggc
     8821 aacagcatcg gacaattatt taaagtgacc actttcggcg aatctcacgg tattgcatta
     8881 ggctgtatcg tagatggcgt accgccgaat atggctttaa gcgaagcaga tttacagccg
     8941 gatttagacc gccgtaaacc cggtacttca cgctacacca cgcctcgccg tgaagatgat
     9001 gaagtgcaaa ttttatcggg agtctttgaa ggcaaaacga cgggaacaag cattggttta
     9061 atcattaaaa acggtgacca acgctcacaa gactatggcg acattgccga aaaattccgc
     9121 ccggggcacg cagattatac ctatcagcaa aaatacggca ttcgagatta ccgtggcggt
     9181 gggcgttctt ctgctcggga aaccgcaatg cgggttgccg ccggggcgat tgccaaaaaa
     9241 tatttgcgtg agcaatttgg cattgaagtg cgaggctacc tctcacaaat cggcaatatc
     9301 aaaattgacc cgaaaaccgt tgaaaatatt gaacaaattg attggcacgc agtcaatagt
     9361 aatcctttct tctgccccga tcccgttgcg attgaaggct ttgatgcctt aattcgtgag
     9421 ctgaaaaaag agggcgattc ggtgggggca aaattaaccg tgattgcaga gcgtgtacct
     9481 gtagggttag gcgaacctgt gtttgaccgc ctagatgccg accttgccca cgccttaatg
     9541 tcgatcaacg ccgtgaaagc ggtggaaatt ggcgatggtt ttgcagttgt ggaacaacgt
     9601 ggtagccagc accgagatga gatgacgccg caaggctttc tctctaatca cgccggcgga
     9661 attttaggtg gtattagctc cggtcagccg attatcgctc atattgcgtt aaagccgacc
     9721 tcaagcatta ctattcccgg cagaagcgtg aacaaaaaca acgaagccgt tgaagtgatc
     9781 actaaaggca gacacgaccc ctgtgtaggt attcgtgctg tgccgattgc agaagcgatg
     9841 atggcgattg tactactcga ccatctgtta agatttaagg ctcaatgccg ttaaacacat
     9901 aaaattggaa aaatgaaatt attaaaaaca cttttagtct caagccttgc ttttacagct
     9961 accgcactga atgcaacgca atgggagaaa atcaaaactc cggttcaagg aaaagcacaa
    10021 tctatcggcg gttatagtaa cggctgtatt attggggcac agccccttgc attaaaaggc
    10081 gaaggctacc aagtgatccg ctcggctcgt aaccgctatt acggacaccc acaactgctc
    10141 gattatctca ctcacttagg caaaaaaacc aaagctgccg gtgtgccgac cattttaatc
    10201 ggcgatatgg gaatgcctgc cggtgggcgt tttgcctccg gtcacgcctc ccatcaaacc
    10261 gggctggatg ctgatatttg gtttaagttc ggaccgatgg acgatgaaac cgcccgcaat
    10321 ccagcagggc ttgccaccat tatggtaaac cgtagtaccg agcgggttga tgattcactc
    10381 tggaatcaac accatactaa tttagtaaaa cttgctgcaa ccgatcctcg agtggatcgc
    10441 atttttgtca atcctgccat taaagtgaaa ctctgccaaa ccgcaggcaa tgatcgtgct
    10501 tggttacgca aaattcgccc gtggtacgga cacgactccc atttccacgt ccgtttaacc
    10561 tgcccggctg atgccgcaag ttgtgaaaat caagcccttg tgcctgccgg tgacggctgt
    10621 ggtgaagagc tttactcgtg gtttgagccg aaagaagcct ccggcacacc aaaagccaaa
    10681 gtgctaccca cagcgcctgt tcaatgtcag atgattttat ctgaacaagg cttgaattaa
    10741 ttgcaaaata acaacctttt ttgaccgctt gtcagcccac aagcggttca ttttcttaaa
    10801 taatttacga ttatgcgttt atttgttgcc gaaaaaccca gccttgcccg agcgattgcc
    10861 gatgtgttac caaaacccca taccaaagga gatggcttta tccaatgcgg cgaacacgac
    10921 actgttactt ggtgtatcgg tcatttattg gaacaggctg aacccgatgt ctatgatgag
    10981 cgtttcaaaa tgtggcgaat ggaacatttg ccgattgtgc ctgaacaatg gaaattgatt
    11041 cccaaaaaag agacgctcaa gcagtttaaa gtggtggaaa agctgattaa aaaagccgat
    11101 gttttagtca atgccggaga cccggatcga gaagggcagc tgttggtgga cgaagttttt
    11161 ggctatttga atttatctgc tgaacgcaaa agccaaattc agcgttgttt ggtaagcgat
    11221 ctcaacccga atgcggtgag ccgagccatt gaaaaattgc aaaaaaacag cgattttata
    11281 ccgcttgcaa cctctgctct cgcccgagcc agagcggatt ggctgtatgg tatcaatatg
    11341 acccgagcct ataccctaca ggggcgttgg gcaggctata aaggcgtgtt atcagtcgga
    11401 cgtgtgcaaa cgccggtatt agggctgatt gttcgccgag atctggagat cgagcatttc
    11461 gtgccaaagg attattttga ggtgttggca cagattgtcg tgccggaaac ccaagagcga
    11521 tttaaggcgc agtggcaacc gagcaaagcc tgtgaagatt atcaagatga agatgggcgg
    11581 gtgctgtctc gtccattggc agaaaatgtg gtaaaacgga ttaccgacca gccggcaacc
    11641 gtcaccgaat atcaaaataa aattgaaaaa gaaactgcac cgctgcctta ttctctttcg
    11701 gtattacaaa tggaagccgc cggcagatat gggctatccg cccaagcggt gttggatatt
    11761 tgccaaaagc tgtatgaaac ccataaacta attacctacc ctcgttctga taatcggtat
    11821 ttgccgaccg aacatttcaa cgacagattt aaagtgatgg cggcgattgc tcatcattta
    11881 cctgaatatc aacaaaaacc ggaagtgatt gatgccacta ttcgcaatcg ttgctggaat
    11941 gataaagagg tggaagccca ccacgccatt attcccactg cacggcaagg gacggtacat
    12001 ttaaccgaaa atgaaaaaag agtttatcaa ctcattgccc gccaatatct aatgcaattc
    12061 tgccctgatg cggaatatcg caaaagtaaa atcgggcttg aaattgcagg tggtagtttt
    12121 gtcgctcaag cacgcaacct tgtaattgcc ggctggcgag cgttactggg taaagaagat
    12181 agccttgacg atgcagagcc actactaccg attgtcaaaa aagggcaaat cctccattgt
    12241 gaaaaagggg aaattgtcag caaaaaaaca caaccgcccc gccactttac tgatcgcagt
    12301 ttgctttcag caatgaccgg gatcgcccgt tttgtacaag ataaagaatt gaaaaaaatc
    12361 ttgcgtgaaa ccgatggttt aggcacggag gcaacccgag ccggtattat cgaattgctg
    12421 tttaaacgtg gttttttaat ccgtaaaggg aaaaatatcc acagtaccga agccggcaga
    12481 atattgattc aagcacttcc cgactctgct acccagccgg atatgaccgc tcattgggaa
    12541 atgcagctga atcaaatcag caaaaaagag accagctatc agcaatttat gtttgatcta
    12601 aaccaacagc tcccacattt attacaaagc ccaaaccgcc aagtgttgca aaatttagcg
    12661 aaaatcacac cgcttgcaag cggcaataaa cctcgttatt ttaaaaagcg aacacaaact
    12721 gctgaaaatt aaatcaagca ggtaaaaatt actgtttagc acttgtcaaa cgggcgtaaa
    12781 ttctatatta tgcacgccat tgcaaatttc gcataaagaa tacaagagaa taaattaatg
    12841 ttaattaatg tactcactat tgtttatctt gtagttgccg tattactgat cgcatttgta
    12901 ttaatgcaac aaggtaaagg cgctgatgca ggtgcttctt tcggggcggg tgcttcagca
    12961 acagttttcg gctcagcagg atcagcaaat tttttatcaa gaacaactgc tctacttgca
    13021 acgattttct tcgcaattag tttagttatc ggtaatttaa gctcacacaa tgtagcaccg
    13081 aaaagccaat ttgaagactt aagccaagtt gaacaaaagc aagtagaacc tgtaaaaata
    13141 gaaaatagcg atatccctca ataatcgctt aacattaaaa agaataaagc tctggtggtg
    13201 gaattggtag acacgccatc ttgagggggt ggtggacaca gtccgtgcga gttcaagtct
    13261 cgcccagagc accattcaac taaatttggc tcaacggagc taaactaaaa aagccttaga
    13321 aagcctgtaa aatcaacatt tacaggcttt tttactatct taactcaacg gctctaaacg
    13381 tttcctatca caaattttag ttacacgttt agttacacgt tttaaatcgt gttactatac
    13441 tttcaataat cgtgtaacac ttcgtaggtt taatttatgg ctagaacaac taaagcatta
    13501 aataaaactc aaatagaaaa tgccaaatca aaggataaag aatatatttt atccgatggc
    13561 gatggattaa atcttagagt taaacctctc cctaaaggct ctaaaatttg gatttttaac
    13621 tacactaacc cacaaactaa aaacagaaca aatttaacta ttgggcgtta ccccttaatt
    13681 acacttgcgg aagctcgcac tatatgtgat ggctaccgct cgttaataga gaaaggcata
    13741 gatccacagg aagaaaaaaa gcggctgcaa caagaagcag aaaatcgagt aaatgatact
    13801 ttccggaatg tggccgaaag ctatttcaat ggtatttata aagagaaagc aaaaaatccg
    13861 attactcgtg aaaagaattg gataaggtta gaaaatcata ttttccccta tatcggggat
    13921 aaacacgttg aagaaatcaa agtaaaagcc cttgttgata tttattctaa agtggcagat
    13981 aaaagcaaca cattaaaaaa actgcatcag cttgtatctg ctgtaatgga tcacgccatt
    14041 acgcaaggca ttatagaaag ccataactgc cgattagcag ttaaaaactt ttatatcaag
    14101 ccatctacgc caaatcccac tattgagctt gaagaattac cgcagttatt tactgattta
    14161 cataatgcga agttatcaaa gcaaacttac gcccttgttt gttggtcgtt cttgactgct
    14221 ctacgcccaa atgaagccgt taatgctgaa tggaaagaaa tagactttga gaaaaagcta
    14281 tggaatatcc ctaaagagaa gatgaaagga aaagcagata aaaaacgccc tcactctgtg
    14341 ccgttatcct cccaagcgtt aaagatatta gagatgatga aaatgcactc taattctagc
    14401 ccttttgttt ttccaggtcg ttcatcaaat agtaaaccaa tgagcaacgc aacggtaaat
    14461 tcaacattaa aaaggaatgg atataaagat agactaaccg cacacggtat tcgggcattt
    14521 gtgaaaacat ttctatcttc tcgcaaagta gatcgccacg tttccgaaag tatattatcc
    14581 catttattag aaggtggaga tgatttagaa aacacataca accgatatga ttttttagaa
    14641 gagcgtgtgc cagtaatgca gcttttaggt gattattgcg aacagtgcgg aatgaattta
    14701 gagttagttc ggggttaggg aggtaaaata tatgatttta gtcgtcacgt catcactttt
    14761 cccactttcc cctttaaaat caaatagtta agtggtgatg actggtttta aagtcatcat
    14821 taagtcatca caaaaaggtg atgacctaga aaaaaagagg ggtttccccc tctttgctaa
    14881 ttcaaaatat cactttgaaa gtcatcaaaa tttttgaaat gaacgtttgt tcgttgccct
    14941 ttattcgtct ttcgttttgt gaaatcgtgc ttattgccgt gttgctttac tgcttgctca
    15001 atacctacta caaaagtatt taagcctagt tctagcacat tcttagcctt aacaaaggct
    15061 aaatatgccg gataaaggtg cgttcttatt ttgtcgtgtc caagcgtgtt gccagttccg
    15121 atataaagcc cgtctgtatt ttctgttgta tatagatact cgaaaaaatc cgttaatggg
    15181 tcagactgct ttttaatctc caacgcctct aggctctctt tttgctcctc taaggctctt
    15241 ttagcttcga gtgggtcagg gaaggtatca agcactttac ggataatgcc ccctatctct
    15301 aatgtaattt tatccataaa atacggatca cgttcatttt caggcggtct tttattaaaa
    15361 cggaaattga caatccgcct atctataccg ccattccgtt ctgtccattt acaagggtcg
    15421 ttattgacga tcataatcat tgccgcaatt cttgcgttaa atgggtgctt gttcttcgga
    15481 tcgactcgca ccatatcacc gccagtaatc gctttcaatc cgccaccatc tccagcgtat
    15541 ttcgtttgct cggaacagat aatcagcttt ttatcttcca gccctgcgat tttgcgttca
    15601 tcatcaaagt tttctagcct tgctgaagct gtgttctttt ctccagctaa tagtgtcgca
    15661 atgccagcaa ataccgattt accgcttccg cctttccctg ttacttggaa gaatacttgc
    15721 cagttgtagc ggtttgttaa aatcgcatag agagcggcta gaatgttctt cgccttgtct
    15781 tgcttgccgt ctgatacaaa gttaagccat tgatcaaagt gtggtgtttc ggctacgttc
    15841 tcgtcatagc tatgtggaat ataagccgtt agccagtttt ggcggttaac ggttctttga
    15901 acatttaccg aataaggtgg gtaaggggtt aaagtttcgg gttgcccctt gctattccaa
    15961 ccaatcaccg tagccggttg tacttccatt ttttgaagcg tgccgaatgg tcgggtttta
    16021 tcgttggctg tcgtgcgtgg ataagctaag aagcctttag cttgttttgt gcctgtaccg
    16081 ttggttaaat cggtttcttc agtttcgaca aaggattctg aaatttcttc agtcagccag
    16141 cctagaatat ctacaccacc aaagtctaaa atctcttgcg tggttttcgg gtaagcgtaa
    16201 atattatgaa cggcaatcgt tacttcattc attttcgggg tggcggtttc ggtgcgtgct
    16261 tcgccttcac cgatatgatt taccgctgca ccgccagccg atactaattt tttatattct
    16321 ttgccttggg tcagtttcac cacgttagca atttgtcgca taacagaatc atctgttagg
    16381 cgtttcatta cttgcttgtc aagttccgga atcactgaat agccaccgtc tgcccctcca
    16441 tttgtattcg tagcaagcga acgcaattcg cctgttttaa tccagtggcg aagctcgtca
    16501 tcagatactt tcacatttgc ggtttcaacc tgttgccccg gttggttacg ctgttcatca
    16561 gtaatcgctt cataacgtgc gatttctttt actaaatttt cagcttgtga gcggagttca
    16621 tcaaacttag ccccttctgc ttcagttgtt gagcgttttt cttcgtctgc tttggttaaa
    16681 attgaacgca tttctgcttc aatagccgct ttttgttggc ggagttctaa aagtttttta
    16741 aacattgcct tatccttaat tcaaataatt gatattgtag tgaagcgttg ctgttttcca
    16801 cgctgattcg ttagcatcat aatcatagct atacttagcc agcacacatt cgtccatact
    16861 gtggaatgtg gcttgattca tcacatcact gattttttga gccactaaat ctaggtcgtt
    16921 ttcggttgaa aacattggta agtaaatgcc gattttcaaa taggcgttgc attcgccatc
    16981 gcaaagcgtg agttggctat attccgcttc atcaatgaat acggcgatag ccggtaattc
    17041 attttctaca tcggtgaata cggctcggcc gttatggaaa tgttgaatgc cttgaatgtt
    17101 accttgtaat aagtcgataa cttcttggcg gattgcttcg ttagaaatca taaaaaagtc
    17161 ctcttaatta agaaggctac aattagccac agaacaatat aggcataatt tcaacggagt
    17221 aaatagggta aaatttaatc gtttagctac gtttgaacac gttaagagga taatttttaa
    17281 atgattattt tttattcaac aaaagacaaa atggaaggaa ataaa
//
LOCUS       DANLRK010000035        16867 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.035, whole genome shotgun sequence.
ACCESSION   DANLRK010000035 DANLRK010000000
VERSION     DANLRK010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 16867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16867
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.035"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            <1..1715
                     /gene="argS"
                     /locus_tag="PXA33_000842"
     CDS             <1..1715
                     /gene="argS"
                     /locus_tag="PXA33_000842"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011200504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HDL5120188.1"
                     /translation="ILSDKIKQAMVASGADSNVEPLVRQSGKPEFGDYQANGAMGAAK
                     KLGMNPREFAQKILENADLNGIADKLEIAGPGFINIFLNPEWLAENTNSSLQAVNFGI
                     KTANPQTIVIDYSSPNVAKEMHVGHLRSTIIGDAVVRTLAFLGNNVIRANHVGDWGTQ
                     FGMLIAYLEKMENEHASEMELSDLEAFYRAAKEHYDSDEAFAEKARNYVVKLQSGDKY
                     CLTMWKKLVDITMHHNQENYNRLNVTLTEKDVMGESLYNPMLPEIVADLKAKGLAVED
                     DGALVVFLDEFKNKDGDPMGVIVQKKDGGFLYTTTDIAAAKYRYETLKADRALVFSDS
                     RQAQHMQQAWLITRKAGYVPDSFSLEHPFFGMMLGKDGKPFKTRSGGTVKLKDLLDEA
                     VERADKLIAERSTELTAEEKAAVVEAVAIGSVKYSDLSKNRTTDYVFDWDNMLTFEGN
                     TAPYMQYAYTRIRSIFARANIDENALSGSIQLNEPKERALAVKLLQFEEALNGVAKEG
                     MPHILCQYLYELAGSFSSFYEACPILNAEETTKNSRLKLAALTAKTLKQGLDLLGIKT
                     VEKM"
     gene            1905..3071
                     /gene="cgtA"
                     /locus_tag="PXA33_000843"
     CDS             1905..3071
                     /gene="cgtA"
                     /locus_tag="PXA33_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HDL5120189.1"
                     /translation="MKFIDEALIRVEAGDGGNGCVSFRREKFIPKGGPDGGDGGDGGD
                     VYLIADENLNTLIDYRFEKRYAAGRGENGRSAGCTGHRGQDITLRVPVGTRAIDNDTQ
                     EIIGDLTRHGMKMLVAKGGYHGLGNTRFKSSVNRAPRQKTNGTPGEKRDLKLELLLLA
                     DVGMLGLPNAGKSTFIRSVSAAKPKVADYPFTTLVPSLGVARVGADRSFVVADIPGLI
                     EGAADGAGLGIRFLKHLERCRILIHLVDIMPIDESDPAHNISVIESELYQYSEKLAEK
                     PIWLVFNKIDTIGEEEAAERAKDIAEQIGWEGDYYLISAATGQNVQALTRDIMDFIEA
                     NPREVEEENKAPEEVKFKWEDYHNEAMQNAVEEDWDDDWSEEDDEGIEFIYERH"
     gene            3156..3773
                     /locus_tag="PXA33_000844"
     CDS             3156..3773
                     /locus_tag="PXA33_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HDL5120190.1"
                     /translation="MLSSTSCDLFNIPFFQFAQMKKYCPEEIPRIKAEYKTQWEIWKK
                     LHQEVSRQLGQPFAPPHIEKWCNGWQVRAHFFAYYKYEFNRNSAAILSVILNRRRLQV
                     SLDWHCYRADRSQINVHQYNQWWENLDREKFADFEIWHGSESEYDDFRTVSQMSEQAF
                     TLTNEEDFWCIGKNVEKDDLDNIDVVEFIYQTIRALLPLYEKCHQ"
     gene            complement(3820..5778)
                     /locus_tag="PXA33_000845"
     CDS             complement(3820..5778)
                     /locus_tag="PXA33_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HDL5120191.1"
                     /translation="MIAELGNYTLALALAISILLAVLPLWGAEKQNSTLIALARPMTW
                     AMFLALTVSFGSLFYLFAVNDFTVQYVVNNSNSRLPLQYRLSAVWGAHEGSLLLWVWL
                     LSLWSVGVATFTRKMPEDAVARVLGLMGVIGIGFLMFVIFTSNPFDRTFPNFPIDGKE
                     LNPMLQDVGLIFHPPLLYMGYVGFSVAFAFAIASLMTGRLDTAWARWSRPWTMAAWVF
                     LTLGIVLGSWWAYYELGWGGWWFWDPVENASLMPWLAGTALIHSLAVTEKRGTFKAWT
                     VLLAILAFSLCLVGTFLVRSGILVSVHAFASDPTRGLYILAYLVLVVGGSLLLYAFKG
                     SEIKSLDNYERYSRETMLLINNILLMAFLSVVFLGTLLPLIHKQIGLGTISIGAPFFD
                     QMFLILMVPFSFVLGIGPLVKWRRDQVSAIRKPVIIATILMALLGFGLPYLFRNTITV
                     SVVLGTMMSVFIVLLSLYELHQRATHRHSFLSGIFKLSRSHWGMVLAHLGVAMTVFGI
                     AFSQNFSIERDVRMNVGDKAEILDYQFEFKGIKITDGANYQGGTAELEITRNGKYEAT
                     LNAEKRFYTVSRMGMTEAAIDWGFTRDLYAALGEKLEDNSWAVRLYYKPFIRWIWIGG
                     LFMALGGLLCMLDKRYRLRIENKKEVIA"
     gene            complement(5775..6332)
                     /gene="ccmE"
                     /locus_tag="PXA33_000846"
     CDS             complement(5775..6332)
                     /gene="ccmE"
                     /locus_tag="PXA33_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HDL5120192.1"
                     /translation="MNPRRKSRLKVVVSILLGLSVAAGLTLYALSQNIDLFYTPSEIV
                     NGKNDDPKQKPEVGQRIRVGGMVVEDTVKRDDKSLKVVFDLNDIGPSITVEYEGILPD
                     LFREGQGIVAQGVLIEPTRLKATEVLAKHDENYMPPELGDKLKEQHKKSGVSEQDLKG
                     ESETDRKMKAEAEAEKANNVQGESK"
     gene            complement(6518..6715)
                     /gene="ccmD"
                     /locus_tag="PXA33_000847"
     CDS             complement(6518..6715)
                     /gene="ccmD"
                     /locus_tag="PXA33_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HDL5120193.1"
                     /translation="MTFQFESISDFFAMGGYGFYVWLSYGVSFIAMIGLIWLSRREQK
                     AILAQAKKEIVREEKLKQTSQ"
     gene            complement(6730..7467)
                     /locus_tag="PXA33_000848"
     CDS             complement(6730..7467)
                     /locus_tag="PXA33_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease"
                     /protein_id="HDL5120194.1"
                     /translation="MWKWLHPYAKSETQYHLLGKIQPFLGWLSLIMLAAAFVWGLAFA
                     PKDYQQGDSYRIIFIHVPAAIWSMGVYGSMAVAGLVALVWQIRQANLAMISMAPIGLV
                     FAFISLATGAIWGKPMWGTWWVWDARLTSALVLFFLYIGVMALYSAFQDRQTGAKAAA
                     VLSVVGVINLPIIHFSVEWWNTLHQGATITKFDKPSMAVEMLIPLLLAIFGSLIFTAW
                     FSIWRYRIALLKDERNRPWVKQLAKSV"
     gene            complement(7667..8329)
                     /gene="ccmB"
                     /locus_tag="PXA33_000849"
     CDS             complement(7667..8329)
                     /gene="ccmB"
                     /locus_tag="PXA33_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005565617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HDL5120195.1"
                     /translation="MILLTIIQRELTIAFRKPSEILNPLWFFLMVITVFPILMGPNPE
                     LLGKIAGGIAWVAALLSALLSFERLFRDDYLDGSLEQLMLLPLALPQVALAKVIAHWL
                     LTGLPLILLSPIAAILLSLETHVWWALVLTLLLGTPILSCLGAIGVALTVGLRKGGTL
                     LSLLILPLFLPVLIFAAAVLEAATLNMGYSGQLAIIGAILAVTLTFSPFAIAGALRIS
                     LN"
     gene            complement(8326..8967)
                     /gene="ccmA"
                     /locus_tag="PXA33_000850"
     CDS             complement(8326..8967)
                     /gene="ccmA"
                     /locus_tag="PXA33_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HDL5120196.1"
                     /translation="MNQTAQYQLKLNQIACERGETRLFEGCSFTVQSGEWVQIEGHNG
                     IGKTSLLRILAGLFLPAEGEVLWNDIAIQKQRDHYYSELFYLGHYSGVKPELTPWENL
                     RFYQKIQQLPQDDDALWHALTKVSLIGREDLPCSHLSAGQQRRVALAKLWLTNAKLWI
                     LDEPFTAIDKKGVADLIAHIEQHCEQGRLVIFTSHQAAESDKVRIISLDQFKV"
     gene            complement(9111..9821)
                     /gene="hda"
                     /locus_tag="PXA33_000851"
     CDS             complement(9111..9821)
                     /gene="hda"
                     /locus_tag="PXA33_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HDL5120197.1"
                     /translation="MQLPLPIHQIDEDTFDNFYSQNSEVLLGSLKRNFTDVQQPFFYI
                     WGGKSCGKSHLLKAVSNHFLLNNQTSSYIPLTKANYFSPLVLDNAELLNVICLDDIQA
                     IAGDAEWELAIFNLFNQIREQQGLFNEGQKTLLLISADCPPHQLNIQLPDLRSRLTWG
                     EVYQLNDLTDEQKCQILQLNAHQKGVELSDEVAHFLLKKAGTDLEQLSKILDKLDKAS
                     LQAQRKLTIPFVKEILSL"
     gene            10091..11692
                     /locus_tag="PXA33_000852"
     CDS             10091..11692
                     /locus_tag="PXA33_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL5120198.1"
                     /translation="MKNMTKLSLITLALAASSSVFAAPKTFVYCLEASPSALSPVLST
                     DGASFDVNALPIYNKLTDFEDGTTNVIPSLAEKWDISEDGKTYTFHLRKGVKFHDNKE
                     FKPSRELNADDVVFSINRQFDPNHPFHKVSGGNYEYFIGMDMQNIIDKVEKVDDHTVK
                     ISLKVPNAPFLANLAMDFNAVYSAEYAEQSLKAGKPERIDTNPIGSGPFQFVDYQKDA
                     TVRYKAFENYWQGTAKIDRLVFAITPDASVRLAKLQKGECHAMPYPNPADIEDLKKDP
                     NIELMSQSGMNIGYLALNSSIKPLDNQKVRQALNHAVNKQAIVDAVYQGAGQVAKNPI
                     PPTMWSYNEAVQDYDYNPEKAKALLKEAGFENGFDTELWAMPVSRPYNPNARRMAELI
                     QEDWKKVGVNAKIVSYEWGEYLKRLRQGEAATSMIGWTGDNGDPDNFLNTLLSCSAVE
                     AGSNYAKFCHKPFEEVVLGAAQESDKAKRTELYKQAQVIFKEQAPWITIAHSTVYFPV
                     RKEVKGYVMSPFSLHNFYKVDLADK"
     gene            11964..12968
                     /locus_tag="PXA33_000853"
     CDS             11964..12968
                     /locus_tag="PXA33_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005712947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDL5120199.1"
                     /translation="MFSFILKRVLMVIPTFIAITLITFALVHFIPGDPIEIRMGERGL
                     SPEVHQQMMEQLGLNRPLFEQYISYIKGVLHGDLGNSFRNNEPVLKEFFTLFPATVEL
                     AFFALLWSLIFGIILGVIAAVKKESWISHTVTTLSLTGYSMPIFWWGLILILYFSTPL
                     GLPASGRLPSEYWIEAETGFMLIDTWNSDEPGAFLAAIKSLILPSIVLGTIPLAVVTR
                     MTRSSMLEVLGEDYIRTAKAKGLNTTRIVIVHALRNALIPVVTVVGLIVGQLLSGAVL
                     TENIFSWPGIGKWIIDAINSRDYPVLQGSVLIIATIIILVNLTVDLLYGIVNPRIRHS
                     "
     gene            13070..13963
                     /locus_tag="PXA33_000854"
     CDS             13070..13963
                     /locus_tag="PXA33_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDL5120200.1"
                     /translation="MSGITAVNPPAPKTPLQEFWYYFCQNRGALIGLTFIAIVFVACV
                     FAPWVAPFDPIEQNRSALLLPPAWLEGGNSAHLLGTDDIGRDILSRIIYGSRLSVFIG
                     LVIVLLSCVFGVILGLLAGYYGGTLDIIIMRFVDIMLAIPSLLLTIGVVTILGPSLIN
                     AAIAIAIVSIPSYVRLTRASVLSEKNRDYVTASRVAGASVLRLMFVVILPNCLAPLIV
                     QMTMGISNAILELAALGFLGIGAQPPTPELGTMLAESRGFMQSANWLVTIPGLAILSL
                     VLAFNLMGDGLRDALDPKLKQ"
     gene            13974..14963
                     /gene="dppD"
                     /locus_tag="PXA33_000855"
     CDS             13974..14963
                     /gene="dppD"
                     /locus_tag="PXA33_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HDL5120201.1"
                     /translation="MALLTVDQLSVHFGETKTPFKAVDRISYEVNEGEVLGIVGESGS
                     GKSVSSLAIMGLIDFPGRVMAEKLYFNGNDLLALKPKEKQQIVGADVSMIFQDAMTSL
                     NPSYTVGYQIMEALKVHQGGSKASRRERAIELLTMVGIPDPESRLEVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIDLLLELQRKENMALILITHDLALVA
                     ESAHRIIVMYAGQVVEEGKAEQIFKAPLHPYTQALLKALPEFAEGKSRLQSLPGVVPG
                     KYDRPQGCLLNPRCPYATEKCRTIEPAMQIINGRQVKCHTPLNAQGEPIRIAV"
     gene            15091..16080
                     /locus_tag="PXA33_000856"
     CDS             15091..16080
                     /locus_tag="PXA33_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL5120202.1"
                     /translation="MKENNSLPPFLKAVDLKKYYPVKKGMFAKPKTVKALDGVSFTLE
                     RGKTLAIVGESGCGKSTLGRMLTMIESPTEGELFYNGQNFLVNDKETAQLRRKKIQIV
                     FQNPYSSLNPRKKVGSILEEPLLINTNLSAKERKEKVLEMMAKVGLKPEFYDRYPHMF
                     SGGQRQRIAIARGLMLQPDIVVADEPVSALDVSVRAQVLNLMMDLQEEMGLSYVFISH
                     DLSVVEHIADEVMVMYLGRCVEQGDTKTIFSNPRHPYTQALLSATPRLNPEQRRERIK
                     LTGELPSPLNPPKGCAFNARCRFATDRCYVERPELKTYQDGTRIACFVVEENN"
     gene            complement(16314..16859)
                     /locus_tag="PXA33_000857"
     CDS             complement(16314..16859)
                     /locus_tag="PXA33_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005758802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL5120203.1"
                     /translation="MSSEQLADIRQQMLKFAQLQLQDIALAEDMVQEAFVNAIKNIDN
                     FQRKAALKTWIFAILKNKIIDYLRKKDRIVLESDLADDDEENHFFDNYGHWKEEYYPS
                     SWQEEAVYSKEFWILFEVCLSNLPAKQARVFMMREYLEATSDEICRVVNITSDNLYTL
                     IYRARLQLQQCLSRKLTERNR"
BASE COUNT         5035 a         3715 c         3582 g         4535 t
ORIGIN      
        1 aaattttatc agataaaatt aaacaggcaa tggtagcaag cggtgcagac agcaatgtcg
       61 agcctttagt ccgccaatcg ggcaagccgg aatttggcga ttaccaagcc aatggtgcga
      121 tgggtgcagc gaaaaaattg gggatgaatc cgcgtgaatt tgcccaaaaa atcttagaga
      181 atgccgattt gaacggtatt gccgataaat tagaaattgc cggtccgggc tttatcaaca
      241 ttttcttaaa cccagaatgg ctggcggaga acactaacag ttcattgcaa gcggtcaatt
      301 ttggcataaa aactgcaaat ccgcagacga ttgtgatcga ctactcctcg ccaaacgtag
      361 caaaagaaat gcacgtcggg catttgcgtt ccaccattat tggcgatgcg gtagttcgta
      421 cccttgcgtt tttaggcaat aatgtgattc gtgccaacca cgttggcgac tggggtactc
      481 aattcggtat gctgatcgct taccttgaaa aaatggaaaa tgagcacgcc agcgaaatgg
      541 agctaagcga tttagaagcc ttctatcgtg cggcaaaaga gcattatgat tcagacgaag
      601 cattcgccga aaaagcccgt aactatgtgg tgaaattaca aagcggcgat aaatattgct
      661 taacaatgtg gaaaaaattg gtcgatatta caatgcacca caatcaagag aactacaatc
      721 gcttaaatgt tacgctcacc gaaaaagatg taatgggcga aagcctctac aatccaatgt
      781 tgcctgaaat tgtggcggat ttaaaagcca aaggcttagc ggtggaagat gacggtgctt
      841 tagtggtttt cctcgatgaa tttaaaaata aagatggcga cccaatgggc gtaatcgtgc
      901 agaaaaaaga tggcggtttc ttatatacca ctaccgatat tgcggcagca aaatatcgct
      961 acgagacctt aaaagctgac cgagccttag tcttttctga cagccgccaa gctcaacata
     1021 tgcaacaggc atggctcatt acccgcaaag cgggctatgt gccggacagt ttcagccttg
     1081 aacacccgtt cttcggaatg atgctaggca aagacggcaa accgttcaaa acccgtagcg
     1141 gtggcacagt caaactcaaa gatttattag atgaagcggt agagcgtgcc gataaactga
     1201 ttgccgaacg ttcaaccgag ctaactgcgg aagaaaaagc cgccgtggtt gaagcggttg
     1261 cgattggttc ggtgaaatat tctgacttat cgaaaaaccg caccaccgat tatgtgttcg
     1321 attgggacaa tatgctgacc tttgaaggca ataccgcccc gtatatgcag tatgcctaca
     1381 cccgcattcg ctccatcttt gcccgtgcga acattgacga aaatgcattg agcggttcaa
     1441 tccaactcaa tgagccaaaa gagcgtgcat tagcagtgaa actattgcaa tttgaagagg
     1501 cattaaacgg cgtggcaaaa gaaggtatgc cacatatttt atgccaatat ttatacgagc
     1561 ttgcaggtag tttctccagt ttctacgagg cttgcccgat tctcaatgcc gaagaaacaa
     1621 ccaaaaacag ccgtttgaaa ctcgctgcct taacggcgaa aaccttgaaa caagggttgg
     1681 atttactcgg tattaaaact gtagagaaga tgtaatacaa actctctccc cttgtgggag
     1741 agagaccgct tatgagcgtt cagcgaaata agcagagaga ggggacaagc ggtaattttt
     1801 attaaaattt ttacacattt tcccctctcc ctgatttttc ttctgaacga agaaaaatct
     1861 gtccctctcc cacaagggga gagggcatat aaaaggaaac aaatatgaaa tttattgacg
     1921 aagccctgat tcgtgtagaa gcaggcgatg gcggtaatgg ctgtgtgagc ttccgccgtg
     1981 aaaaatttat cccgaaaggc ggacctgacg gtggtgatgg tggcgatggt ggtgatgtgt
     2041 atttaattgc cgatgaaaac ctaaacaccc ttatcgacta ccgctttgaa aaacgttatg
     2101 ccgctgggcg tggcgagaat ggtcgcagtg ccggctgtac cggacatcgt gggcaagata
     2161 ttaccctacg agtgccggtt ggaacccgtg cgattgataa cgacacccaa gaaattattg
     2221 gcgatttaac ccgccacgga atgaaaatgc tggtggcaaa aggtggttat cacggtttag
     2281 gtaatacccg ctttaaatcc tcagtaaacc gagctccacg ccaaaaaacc aacggtacgc
     2341 ctggcgaaaa acgtgatttg aaattagagc tgttactgct tgccgatgtg ggaatgcttg
     2401 gcttaccaaa cgccggtaaa tccaccttta tccgcagtgt ctctgctgcc aaaccaaaag
     2461 tggcggatta cccgttcacg acgctggtgc caagtttagg ggtagcccgt gtgggggccg
     2521 accgcagttt tgtggttgcc gatattccgg ggttaattga aggtgcagca gatggtgcag
     2581 ggctggggat tcgtttccta aaacacttgg aacgttgccg tattttgatt cacttagtcg
     2641 atattatgcc gattgatgaa agcgatccgg ctcataatat ttcggtgatt gaatccgagc
     2701 tttaccaata cagcgaaaaa ctcgccgaaa aaccgatctg gttggtgttt aataaaattg
     2761 acaccatcgg cgaagaagaa gctgctgagc gagcaaaaga cattgccgag caaatcggct
     2821 gggaaggcga ttactacttg atttctgccg ctaccggtca aaacgtgcaa gctctcactc
     2881 gtgatattat ggactttatt gaagccaacc cacgcgaagt ggaggaagaa aataaagcac
     2941 ctgaagaagt caaattcaaa tgggaagact accacaacga agcaatgcaa aatgcggtag
     3001 aagaagattg ggacgacgat tggagcgaag aagatgacga aggcatagag tttatttacg
     3061 aacgccatta gtgcaataat acaagcggtt gtttttaaac ggagatttgc aaatttccgg
     3121 agaaaaacaa ccgcttgtag gtttaaggag agtttatgct tagctcaaca tcgtgcgatt
     3181 tattcaatat tccgttcttc caatttgctc agatgaagaa atattgccct gaggagatcc
     3241 ctcgaattaa ggcggaatat aaaactcaat gggaaatttg gaaaaagcta catcaggaag
     3301 tttctcgtca gcttgggcag ccgtttgctc caccacatat tgagaagtgg tgcaacggtt
     3361 ggcaagttcg agcccatttt tttgcctatt acaagtatga atttaatcga aattctgcgg
     3421 ctattctttc cgtgatttta aatcgtcgcc gtttgcaagt gagccttgat tggcattgtt
     3481 atcgagccga tcgctcgcaa attaacgtgc atcaatacaa tcaatggtgg gaaaatttag
     3541 atcgggaaaa gtttgctgat tttgagattt ggcacggaag tgagagtgaa tatgatgatt
     3601 ttagaaccgt tagtcagatg tcagagcaag cgttcaccct aactaacgag gaagattttt
     3661 ggtgtatcgg caaaaatgtg gaaaaggacg atttggataa cattgatgta gtagaattta
     3721 tctaccaaac cattcgggca ttattgccac tttatgagaa atgtcatcaa taaacactaa
     3781 aaagagaggc gaacatcatt tgttcgcccc tttcaaaatt taagctatca cttctttttt
     3841 attttcaatg cgtaaacggt agcgtttatc caacatacag agtaaaccac cgagagccat
     3901 aaacagcccg ccaatccaaa tccagcgaat gaacggttta taatacaaac gcactgccca
     3961 agagttatct tctaattttt cgccaagtgc agcgtataaa tctcgggtaa agccccagtc
     4021 aatcgccgct tcggtcattc ccattcggct caccgtatag aaacgttttt cagcattaag
     4081 tgtggcttca tatttaccgt ttcgagtaat ttcaagctcg gcagtgccgc cttgatagtt
     4141 tgccccatcg gtgattttaa tacctttaaa ttcaaattgg taatctaaaa tctcagcttt
     4201 atcacccaca ttcattctga catctcgttc aatgctgaaa ttttggctaa atgcaatgcc
     4261 gaacactgtc atcgcaaccc ctaagtgagc cagcaccatt ccccaatgtg aacgggacag
     4321 tttgaaaatg ccacttaaga acgagtggcg atgggttgca cgttggtgta gctcatacag
     4381 gctaagtaac acaataaaga cagacatcat cgtgcctaac accacactca cggtaatggt
     4441 gttacggaat aagtatggca atccgaatcc aagcaatgcc attaaaatag ttgcaataat
     4501 taccggttta cgaatagcag aaacttggtc tctacgccat ttcactaatg gtccaatccc
     4561 taatacaaat gagaaaggaa ccattaaaat taagaacatt tggtcaaaaa atggtgcccc
     4621 gattgaaatc gtccctaagc cgatttgttt gtgaattaat ggcagtaagg tccctaaaaa
     4681 caccaccgat aaaaatgcca ttaataaaat attattaatg agtagcatcg tttctcttga
     4741 atagcgttca tagttatcta agcttttaat ttccgagcct ttgaatgcat acagcagtaa
     4801 agaaccacct actactaaca ccaagtaagc caagatgtat aaaccacgag ttggatctga
     4861 agcaaaggcg tgaactgaca ccaaaatgcc tgaacgcact aagaaagtcc ccactaaaca
     4921 gagcgagaaa gcaagaatcg ctaataagac cgtccacgct ttaaatgtgc ctcgtttttc
     4981 agtcactgct aatgaatgaa tcagtgctgt tcctgcgagc cacggcatta atgaggcatt
     5041 ctcgacagga tcccagaacc accagccacc ccaaccaagt tcgtagtatg cccaccaaga
     5101 accaagcacg ataccgagcg ttaaaaatac ccacgctgcc attgtccacg gacgagacca
     5161 tcttgcccac gcagtatcta aacgccctgt catcagtgag gcaatcgcaa aagcaaaggc
     5221 aacggagaaa cccacatagc ccatataaag caacggtggg tggaagatca agcccacatc
     5281 ttgcagcatt gggtttaatt ctttgccatc aatcggaaaa tttgggaaag ttctatcaaa
     5341 cgggttggag gtaaaaatca caaacatcaa aaagccgatg ccaatgaccc ccattagtcc
     5401 taacactcga gcaacggcat cttccggcat tttacgggta aaggttgcta ccccgacaga
     5461 ccaaagcgac aacaaccaaa cccaaagcaa taatgagcct tcgtgagcgc cccacaccgc
     5521 agataaacga tattgcaacg gtaaacggct attggagttg ttcaccacat attgcacggt
     5581 aaaatcattc accgcaaaca gataaaacag cgaaccaaaa gagacagtca atgccaaaaa
     5641 cattgcccaa gtcatcgggc gggcaagagc aattaaggtg ctattttgct tttctgctcc
     5701 ccaaagcggt aaaactgcca acaagataga aatcgctagg gctaatgcca acgtataatt
     5761 gcctaattct gcaatcattt gctttcccct tgcacattgt tggctttttc tgcttccgcc
     5821 tcggctttca ttttgcggtc ggtttccgat tcacctttca gatcttgttc agacacacct
     5881 gattttttat gctgttcttt taatttatcg cctaattccg gtggcatata gttttcatcg
     5941 tgttttgcca gaacttccgt agcttttaaa cgggttggtt caatcaacac gccttgtgcc
     6001 acaatgcctt gcccttcacg gaataaatcc ggcaaaattc cttcgtattc cacggtaatc
     6061 gaaggcccga tatcattgag atcaaatact acttttaaac ttttatcatc acgctttacc
     6121 gtatcttcca ctaccattcc gcccacacgg atacgctgac caacttcagg cttttgtttc
     6181 ggatcatcat ttttaccgtt tacaatttca gacggcgtgt aaaacagatc gatattttgg
     6241 cttaacgcat aaagtgtcaa gcctgctgcg acagaaagcc caagtaatat cgaaaccacc
     6301 acttttaatc ttgatttgcg tcttggattc atcgtaatca cccttcaaaa taaatgcaaa
     6361 attgcataaa aactcaaaaa taaattagtg taagaatagc aaaaaaccgc aaaacattcc
     6421 cgatatttca aggaagtttt gcgatttctc aaatcaaata cctataaaga agtatgaggc
     6481 tatcgttgca aaaaataaag ttatttcgac cgcttgttta ttgtgatgtt tgctttaatt
     6541 tttcttcacg cacaatctct ttttttgctt gagccaaaat cgctttctgt tcacggcgac
     6601 taagccaaat cagcccaatc attgcaatga aactgacacc ataagagagc catacataaa
     6661 agccgtagcc acccattgca aaaaaatcgc tgattgattc aaattgaaat gtcattgttc
     6721 tctcctactc taaactgatt ttgccagttg ttttacccaa gggcgattac gttcgtcctt
     6781 taataaggca atgcgataac gccaaatgct aaaccaagcg gtaaaaatta agctaccgaa
     6841 aatcgccaat agcaacggaa ttaacatttc caccgccata gacggtttat cgaatttagt
     6901 gatggtcgcc ccttgatgca aggtgttcca ccattccacc gaaaagtgaa taatcggcaa
     6961 gttaatcacg ccaacaactg acagcacagc cgccgctttg gctccggttt gtctgtcttg
     7021 gaatgcggaa taaagagcca tcacgccgat ataaaggaaa aataacacta aagcggaggt
     7081 taaacgagcg tcccacaccc accaagtccc ccacattggt ttgccccaaa ttgcaccggt
     7141 tgcaagtgaa ataaaagcga atactaagcc gataggtgcc attgaaatca tcgccaagtt
     7201 agcttggcga atttgccaaa ccaatgccac caagcctgcc accgccattg agccatatac
     7261 gcccatcgac caaattgctg ccggcacgtg aataaagata atgcgatagc tatcgccctg
     7321 ttggtaatct ttcggggcaa aagccaagcc ccaaacaaaa gcagccgcta acataatcaa
     7381 acttagccaa cctaaaaacg gttggatttt ccctagcaaa tggtactgcg tttccgattt
     7441 tgcataaggg tgtagccatt tccacatata tttctccaaa gtaaataatt cccctcaccc
     7501 gtttacagga gagggacgat ttgagcttcg ttcagaagcg aaaatcaggg agaggggaaa
     7561 aagttgcaaa ttttcataat aattcgaccg cttcccctct ctctgaaaaa cacctctgaa
     7621 cgaggtgttt ttcggtctct ctccctcaag agggagagag gaagttttag ttcaaactaa
     7681 ttcttaatgc accggcaatc gcaaacggcg aaaaagttaa agtaaccgct aaaattgctc
     7741 cgataatcgc cagttgtccg ctataaccca tatttaaggt ggcggcttct aataccgcag
     7801 cggcaaaaat cagtaccggt aagaataacg ggaggattaa taaactcagc aacgtaccgc
     7861 ccttgcgtaa gcccacagtg agtgccacgc caatcgctcc taagcagctt aaaatcgggg
     7921 tgccgagtaa taatgtcagc actaacgccc accaaacgtg ggtttcaagt gaaagcaaaa
     7981 tggcagcaat cggtgaaagt aaaatcaacg gtaagccggt cagcagccag tgagcaatca
     8041 ctttcgctaa cgcaacctgc ggcaaggcaa gcggcagtaa cattaattgc tcgagcgaac
     8101 cgtctaaata atcatcacgg aataaccgct caaaggaaag caatgcagaa agcaaagcag
     8161 ctacccacgc aatgccgccg gcaattttgc ctaataattc ggggttaggt cccattaaaa
     8221 tcgggaatac ggtaatcacc attaaaaaaa accacaacgg atttaaaatc tccgagggtt
     8281 tacgaaaggc aatggtgagt tcccgctgaa taatagtcaa taaaatcata ctttaaattg
     8341 atctaacgaa ataatacgca ctttgtcgct ttcagcagcc tggtggctgg taaaaatcac
     8401 taatctgcct tgctcgcaat gctgttcaat atgagcaatc agatccgcca cacccttttt
     8461 atcaatagcg gtaaagggtt catctaaaat ccaaagtttg gcgttagtga gccacaattt
     8521 tgccaacgcc acacggcgtt gctgaccggc tgacaaatgc gaacaaggca aatcttcacg
     8581 cccgatgagc gacactttcg tcaatgcgtg ccaaagggca tcatcatctt gcggtaactg
     8641 ttggattttc tggtagaaac gcaaattttc ccacggtgtt aattccggtt ttacccccga
     8701 ataatgccct aaataaaata gttcagaata atagtgatca cgctgttttt gaattgcaat
     8761 atcgttccac agcacctcac cttccgcagg taaaaaaagc ccggctaaaa tacgtaacag
     8821 gctggttttt ccaatgccgt tatgcccttc aatctgcacc cattcaccac tttgcacggt
     8881 aaaactgcac ccttcaaaca aacgggtttc gccccgttcg caggcaatct gattaagttt
     8941 aagctgatat tgggccgttt ggttcattat ttttcatctg gttgaacatt ttagccgaag
     9001 tgtatcataa gcctttgatt taacgtagca aataaattac ttctttagag gtagcaagcg
     9061 gtcaaatttt caaaaagttt tgcaaatttt gataagaatc aaaccgcttg ttacagagag
     9121 aggatttcct tcacaaaagg aatggttaat ttacgctggg cttggagaga cgctttatcc
     9181 aacttatcca atattttaga gagttgctct aaatccgttc ccgctttttt taacagaaaa
     9241 tgagcgacct catcggacaa ttccacccct ttttggtgag catttaactg taaaatttgg
     9301 catttttgtt catcggttaa atcgtttagc tggtacactt cgccccaagt taagcgggaa
     9361 cgtaaatccg gcaattggat attgagttgg tgtggtgggc aatcggcact gattaacagc
     9421 aaggtttttt gcccttcgtt aaacaagcct tgttgttctc gaatttggtt aaacaggtta
     9481 aaaatagcaa gttcccattc agcatctcct gcaatcgctt gaatatcatc taggcaaatc
     9541 acattcagca gttcggcatt atctaacacc aacggcgaga aataattggc tttagtgagc
     9601 ggaatataac tagaagtttg gttatttaaa agaaagtggt tacttactgc tttcaacaaa
     9661 tgacttttac cgcagctctt accgccccaa atgtaaaaaa acggctgttg aacatccgta
     9721 aaatttcgtt tgagggatcc aagcaacact tcgctgtttt ggctataaaa attgtcgaaa
     9781 gtgtcttcat caatttgatg aatgggtaga ggtaactgca aggctttcct taaatctcta
     9841 agtttgatat ttttctaaga atagcggaaa agcaggggtt tgagaagtaa aaatagtgag
     9901 aaaatatcca ctcatttttc taatgaagag atgacaagcg gttgaatttc cacataattt
     9961 tgtcaatctg accgaaaacc caccgttttg tgttaaaaaa agattgcagt tagctcaaaa
    10021 aattgtatat tcaaagcttt ctacttaaaa agtagagtaa aacaactatt acaaacacaa
    10081 cggagtgaat atgaaaaaca tgactaaatt atcgctaata acattagcgc tagccgcttc
    10141 aagtagcgtc tttgctgcac cgaaaacctt tgtttactgc ttagaggctt cgccttcagc
    10201 attaagccca gtgctatcaa cagacggtgc aagctttgat gtgaacgccc tcccgattta
    10261 taacaaatta accgactttg aagacggtac aactaatgtt attccctctc ttgccgaaaa
    10321 atgggacatt tcggaagacg gcaaaactta caccttccac ctgcgtaaag gcgtaaaatt
    10381 ccacgacaat aaagaattta aaccaagccg tgagttaaat gcagacgatg tagtgttctc
    10441 cattaaccgt cagtttgatc caaaccatcc attccacaaa gtttccggcg gtaactacga
    10501 atactttatc ggtatggata tgcaaaatat tatcgataaa gtggaaaaag tggacgatca
    10561 caccgttaaa atcagcttaa aagtgccaaa tgcgccgttc ttagctaact tagcgatgga
    10621 tttcaacgcc gtttactcag cggaatatgc agagcaatcc ttaaaggccg gcaagcctga
    10681 gcgtattgat accaacccaa tcggcagcgg cccgttccag tttgtggatt accaaaaaga
    10741 tgccaccgtt cgttacaaag cctttgaaaa ctactggcaa ggcacagcga aaattgaccg
    10801 cttagtgttt gcaattactc cggatgcatc ggtacgttta gccaagttac aaaaaggcga
    10861 gtgccacgca atgccttacc ctaacccggc tgatatcgaa gatctgaaaa aagatccaaa
    10921 tattgaatta atgagccaat ctggaatgaa tatcggctat ttggcattaa attcatcgat
    10981 taaaccatta gataaccaaa aggtacgtca agcattaaat cacgctgtaa acaaacaagc
    11041 gattgtagat gcggtatatc agggagccgg tcaagtagcg aaaaacccaa ttccaccaac
    11101 aatgtggagc tataatgaag cggttcaaga ctatgactac aaccctgaaa aagccaaagc
    11161 attgttaaaa gaagccggtt ttgaaaatgg ttttgatact gaactttggg caatgccggt
    11221 atcacgtcca tataacccga atgctcgccg tatggctgaa cttatccaag aagactggaa
    11281 aaaagtgggc gtgaatgcga aaatcgtcag ctatgaatgg ggcgaatatc tcaaacgctt
    11341 acgccaaggt gaagcggcaa cctcaatgat tggctggaca ggcgataacg gtgacccaga
    11401 taacttcctc aacaccttat taagctgctc agcggtggaa gccggttcaa actacgccaa
    11461 attctgccac aaaccgtttg aagaagtcgt gcttggtgct gcccaagaaa gcgacaaagc
    11521 aaaacgtacc gagctttata aacaagctca agtgatcttc aaagagcaag ctccttggat
    11581 tacgattgct cactccaccg tttacttccc ggtgcgtaaa gaagtaaaag gttatgtgat
    11641 gagtccgttc agcttacata acttctacaa agtagattta gcggataaat aatccgccaa
    11701 caagcggtag aatttgcaga tttttttaca aatttgaccg cttgtcctcc cctctttgaa
    11761 ctacgagggg caggggggaa atttggcaga agcgattgcc gaagtgagag gattgtaaaa
    11821 gaggaaataa attttctgtc ttattgatta ttttcttcgt taattactac tgtcaaatct
    11881 cccctacccc ctctttgcta aagaggggaa tcagatcgag agatcagaca actttgatga
    11941 aaatccaacc taagagattt taaatgtttt catttatttt aaaacgtgtg ctaatggtga
    12001 tccctacctt tatagcgatt acgttaatta cttttgccct tgtgcatttt atccccggtg
    12061 atccaattga gatccgaatg ggggaacgtg gattatcgcc tgaggttcat caacaaatga
    12121 tggaacaact cgggctgaac cgaccgttgt ttgaacaata catcagctac atcaaaggtg
    12181 tattgcacgg tgatcttggt aactcatttc gtaacaacga accggtgctg aaggaatttt
    12241 ttaccctatt cccagccacg gttgagttgg cattttttgc cctgctctgg tcgttgattt
    12301 tcggcattat tctcggtgtg attgctgcgg tgaaaaaaga aagttggatt tcacacactg
    12361 taaccacgct ttcactgacc ggctactcaa tgccaatttt ctggtggggc ttgattttaa
    12421 ttctttattt ctccaccccg ctcggcttac cggcttcagg tcgtttacca tctgaatact
    12481 ggattgaagc cgaaaccggt tttatgctga ttgatacttg gaattcggac gaaccagggg
    12541 catttttagc ggcaattaaa tctctgattt tgccgtctat cgtactgggg acaatccctc
    12601 ttgcagttgt gacccgaatg actcgctctt caatgttgga agtgttgggt gaagactata
    12661 tccgcaccgc caaagccaaa ggcttaaaca ctacccgaat tgtgattgtt cacgcattaa
    12721 gaaatgcctt gattccggtg gtaacggtcg ttggcttaat tgtcgggcaa ctgctttcag
    12781 gagccgtttt aacggaaaac atcttctcgt ggccgggtat cggcaaatgg attattgatg
    12841 caatcaacag ccgtgattac cctgtactac aaggctcagt gctgattatt gctactatta
    12901 ttattttagt gaaccttacg gtagatctac tctatggcat tgtcaatccg aggattcgac
    12961 atagttagaa aaattccttc gatttttccc ctctttagca acgagggagt tacacaagcg
    13021 gttcaaattc cgcaattttt tacaagatta tttttagttg gagttaatga tgtcaggaat
    13081 aacggcggta aatccacccg cacccaaaac accgttacag gagttttggt actatttttg
    13141 tcaaaatcgg ggggcattaa taggacttac ctttattgca atcgtctttg ttgcgtgcgt
    13201 attcgcacct tgggttgccc cttttgatcc aatcgaacaa aatcgcagtg cgttattgct
    13261 ccctcctgct tggcttgaag gggggaattc cgctcatctg ctgggtactg acgatattgg
    13321 gcgtgatatt ctttcccgta ttatttatgg ttcaagattg tccgtcttta tcggcttagt
    13381 cattgtgcta ctttcttgcg tattcggcgt gattttaggt ctacttgctg gctactatgg
    13441 tggcacactc gacatcatca ttatgcgttt tgttgatatt atgctggcga ttccaagcct
    13501 gcttttaact atcggcgtgg tcactatttt aggcccttca ttgataaatg ccgctattgc
    13561 gattgcgatt gtctccattc caagttatgt tcgcttgacc cgtgcttctg tattaagcga
    13621 gaaaaaccgt gattatgtta ctgcttctcg tgttgctggt gcgagcgtgc ttcgcttaat
    13681 gtttgtggtg attttaccta actgtttagc tccgcttatc gttcagatga cgatggggat
    13741 ctcaaacgct attttagaac ttgccgccct cggcttctta ggcattggtg cacagccgcc
    13801 aacaccggag cttggcacaa tgcttgccga atctcgtggc tttatgcaat cggcaaactg
    13861 gctggtgacc attccgggtc tagcgatttt atcgttagta ttagctttca acttaatggg
    13921 tgacggcttg cgtgacgccc tcgatccaaa actgaaacaa taggagatct aaaatggcat
    13981 tattaacggt tgatcaactt tccgtccatt ttggtgaaac taaaacacca tttaaagcgg
    14041 tggatcgtat cagttacgaa gtcaatgaag gcgaagtatt aggcattgtg ggcgaatccg
    14101 gttcaggaaa atcggtaagt tctctcgcta ttatgggctt aatcgatttc cccggcaggg
    14161 ttatggctga aaaattgtat tttaacggca atgatttatt ggcattaaaa ccaaaagaaa
    14221 aacaacagat tgtaggggct gatgtctcaa tgatcttcca agatgcaatg accagcttaa
    14281 atccgagcta tactgtgggc tatcagatta tggaagccct aaaagtgcat caaggtggct
    14341 caaaagccag ccgtcgtgag cgagcgattg agttgctgac gatggtcggt attcccgatc
    14401 ctgaatcccg tttagaagtt tatccgcacc agctttcggg tggtatgagc cagagagtga
    14461 tgattgcgat ggcgattgcc tgtcgcccga aattattgat tgccgatgag ccgactactg
    14521 cgttagatgt gacgattcaa gctcaaatca ttgatttatt acttgaatta caacgcaaag
    14581 aaaatatggc gttgatttta atcacgcacg atttagcgtt agttgccgag tctgcccatc
    14641 gcattatcgt gatgtatgca gggcaagtcg tggaagaggg caaagccgag cagatattca
    14701 aagcaccact gcacccttac acccaagcat tgctcaaagc cttaccggaa tttgccgaag
    14761 ggaaatcccg cctgcaatca ctaccgggcg tagtgccggg caaatatgac cgcccacaag
    14821 gttgcttact caaccctcgc tgcccgtatg caaccgaaaa atgccgaacc attgaaccgg
    14881 caatgcagat aatcaacggc agacaggtga aatgccacac gccactgaat gcacagggtg
    14941 agccgataag gatagctgta taacgtcata aagagtaggg acgcaatgct tcgcgtccgt
    15001 tacaagcggt gatttttaac cgatttttgc aaaataacga tagtggacgc aaagcattgc
    15061 gtccctacat tttccgagaa ggaaaacaga atgaaagaaa ataattctct cccaccattc
    15121 ctcaaagcag tggacttaaa aaaatactac ccagttaaaa agggaatgtt cgctaaaccg
    15181 aaaacggtaa aagcgctgga tggtgtctct tttacccttg aacgtggcaa aacccttgcg
    15241 attgttggcg aatccggctg tggaaaatca actttggggc gaatgctcac tatgattgaa
    15301 agcccaaccg aaggtgaact gttctacaac gggcagaatt tcttagtcaa tgacaaagaa
    15361 acggctcaac tacgccgtaa aaaaatccaa attgtgtttc agaacccgta tagctcgctc
    15421 aatccacgca aaaaagtagg tagcatttta gaagaaccat tattgattaa caccaactta
    15481 tccgcaaaag aacgcaaaga aaaagtgttg gaaatgatgg cgaaagtcgg cttaaaaccg
    15541 gaattttacg accgttatcc gcatatgttt tcgggcggac aacgccagcg aattgcgatt
    15601 gctcggggct taatgttgca gcctgatatt gtggttgccg atgaacccgt ttccgcatta
    15661 gatgtttcgg ttcgggcgca agtgttgaat ttgatgatgg atttgcaaga agaaatgggc
    15721 ttgtcttacg ttttcatctc gcacgattta tccgtagtgg aacatattgc cgatgaagtg
    15781 atggtgatgt atttagggcg ttgcgtagaa caaggggata ccaaaactat cttcagcaat
    15841 ccacgtcacc cttatacaca agcgttactc tccgcaaccc ctcgtttgaa cccggagcaa
    15901 cgccgtgagc ggattaaact gactggcgaa ctaccaagcc cgcttaatcc accaaaaggc
    15961 tgtgccttta acgcccgttg cagatttgca accgatcgtt gctatgtgga gcgaccagag
    16021 ctgaaaacct atcaagacgg tactcgcatt gcttgttttg tggtggagga aaataattag
    16081 gcaacgctcg ctatttaaaa ggttaaagtt tttcaacttt aacctttatt tttcatactt
    16141 aatactcctc atcttttgta gcaaaatctt tcattaattt gctgatatgc tcacaatggt
    16201 gtttaaatga caatacgggc aaataatcaa atgcgtttta acgccaatct tctcttttaa
    16261 agtgagtttt cgttcctttg agtcagaaat taaccttgtt acttgtcggc aattcatcta
    16321 tttctctccg ttaatttcct tgaaagacat tgttgtaact gcagccttgc tcgatagatt
    16381 aatgtatata aattatctga tgtaatattt actactcgac aaatttcatc tgaggttgcc
    16441 tctaaatatt ccctcatcat aaatacccta gcttgttttg caggtaaatt gcttaagcaa
    16501 acttcaaata atatccaaaa ttctttggaa taaacagctt cttcctgcca gctagaagga
    16561 taatattctt ctttccaatg cccataatta tcaaaaaagt gattctcttc gtcatcatca
    16621 gctagatcac tttctagtac tatacgatct tttttacgta aataatcaat gattttattt
    16681 tttagaatag caaatatcca ggttttcaat gctgctttac gctggaaatt atcaatattt
    16741 tttattgcat taacgaatgc ttcttgaacc atatcttcag caagagcaat atcttgtaat
    16801 tgtaactgag caaatttcaa catttgctga cgaatatcgg caagctgttc cgatgaaata
    16861 tctaaag
//
LOCUS       DANLRK010000036        16754 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.036, whole genome shotgun sequence.
ACCESSION   DANLRK010000036 DANLRK010000000
VERSION     DANLRK010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 16754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16754
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.036"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            <1..406
                     /gene="nudB"
                     /locus_tag="PXA33_000858"
     CDS             <1..406
                     /gene="nudB"
                     /locus_tag="PXA33_000858"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HDL5120204.1"
                     /translation="IYAKNTQRVLMLQRKDDPDFWQSVTGSINIGETPLEAAEREVSE
                     ETGLQTFEQKQPLVDCQKQIEFEIFPHFRYKYAPNITHCVEHWFLLPLDSEQEPILTE
                     HLAYRWVSVEDAVRLTKSPNNAQAIQEYLTSK"
     gene            419..1159
                     /locus_tag="PXA33_000859"
     CDS             419..1159
                     /locus_tag="PXA33_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL5120205.1"
                     /translation="MAGHSKWANIKHRKAAQDAQRGKIFTKLIRELVTAAKLGGGDTS
                     SNPRLRAAVDKALSSNMTRDTINRAIERGVGGGDDTNMETKIYEGYGPGGTAVMVECL
                     SDNANRTISQVRPSFTKCGGNLGTEGSVGYLFSKKGLILVANADEDALTEAAIEAGAD
                     DIQPQEDGSFEIYTAWEDLGSVKDGIEAAGFKIENAEVTMIPSTTVELDAETAPKLLD
                     LINRLEDCDDVQNVYHNGEISDEVAALL"
     gene            1289..1816
                     /locus_tag="PXA33_000860"
     CDS             1289..1816
                     /locus_tag="PXA33_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5120206.1"
                     /translation="MKLVKFVPALAATLVLAACSNANTQSAPQQAQDAVTKVAEQATQ
                     QVNEIAGNAPRASGAVKVEKATDGAKSIVYRCQSGKRVTANYVFQGEEAKAVNLLVQS
                     GGKTQKIPTLTRDASNQDSTSFKTDKYSWDLDTGFTLSSNGQSGMLTKYGAKTDTIVA
                     KLCDVDKALTSKLNK"
     gene            1883..2461
                     /gene="ruvC"
                     /locus_tag="PXA33_000861"
     CDS             1883..2461
                     /gene="ruvC"
                     /locus_tag="PXA33_000861"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HDL5120207.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQNGRQLEYLGSGAIRTTAEDLPTR
                     LKRIYAGVSEIITQFQPNMFAIEQVFMAKNPDSALKLGQARGTAIVAAVNHDLPVFEY
                     AARLVKQTVTGIGSADKVQVQDMVTRMLKLSAKPQADAADALAIAITHAHSIQHSLIV
                     AKQSQQNVSSEKEQILALMKTRYSRGRFRLKG"
     gene            complement(2527..3807)
                     /gene="murA"
                     /locus_tag="PXA33_000862"
     CDS             complement(2527..3807)
                     /gene="murA"
                     /locus_tag="PXA33_000862"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDL5120208.1"
                     /translation="MEKFRVYGPFSLNGTVDISGAKNAALPILFAAILAEKTVTLKNV
                     PDLKDVDTTFKILRQLGVTVEKGSDKGVVHIDASKINNYVAPYELVKTMRASIWALAP
                     LVARFQLGQVSLPGGCTIGARPVDMHISGLEKMGAKIELDEGYVKATSNGRLNGARIY
                     MDKVSVGATLSVMMAGTLAKGTTVIENAAREPEIVDTADFLNAMGAKISGAGTDMITI
                     EGVEHLGGCEHSVVPDRIETGTFLVAAAISGGRITCRNTKADTLDAVIEKLREAGMQV
                     DVTENTITLDSLGRRPKAVNIRTMPHPGFPTDMQAQFTLLNTVAEGTSRITETIFENR
                     FMHIPELIRMGAKAEIEGNIAICYGVEQLKSAEVMATDLRASISLVLAGCIATGETIV
                     DRIYHIDRGYEHIEEKLRQIGARIERFSAPFEGE"
     gene            complement(3917..4177)
                     /locus_tag="PXA33_000863"
     CDS             complement(3917..4177)
                     /locus_tag="PXA33_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HDL5120209.1"
                     /translation="MNPSQIEEILKQALSDATEVHAQGENAHFGVIVVSDSIASLSKV
                     KQQQAVYAPLAEYFTTNAIHALTIKVFSVEKWKTERLLNMVG"
     gene            complement(4210..4533)
                     /locus_tag="PXA33_000864"
     CDS             complement(4210..4533)
                     /locus_tag="PXA33_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HDL5120210.1"
                     /translation="MPQKTLQWDIQQNNESLFVKLSGELTRNTLLPLWKQRASFLSPT
                     GKQSLYWDLKEITRIDSAGFTLLAELLNHYHKIIPNSLINTPDSVRTLAALYDLDGWF
                     EQFII"
     gene            complement(4545..5180)
                     /gene="mlaC"
                     /locus_tag="PXA33_000865"
     CDS             complement(4545..5180)
                     /gene="mlaC"
                     /locus_tag="PXA33_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HDL5120211.1"
                     /translation="MLKNIKKIIVTGLVAVSALFSTTAFAETSPYVLMQQTADKLFGD
                     IKANQAKIKSNPEYLRTIVRNDLMPHVHVKYAGQLVLGKNLSAATDAQKEAFFNAFGQ
                     FVEQSYAQVLTQYQDQSVQIESPKSVDGKSIVSIRVNVSGKGAAQPIKLDFKWRKNSK
                     TGEWQAYDMAAEGVSMVATKQNEWAGVIRQKGIDALIAQVAQSAKQPITLK"
     gene            complement(5226..5750)
                     /gene="mlaD"
                     /locus_tag="PXA33_000866"
     CDS             complement(5226..5750)
                     /gene="mlaD"
                     /locus_tag="PXA33_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HDL5120212.1"
                     /translation="MRQSIKYEFWVGLFVLLGLAALVFLGLRVANVQGFSNEKTYTLY
                     ATFDNIGGLKVRAPIKVGGVVVGRVADISLDNKTYTPKVALAVNESFNQIPDTSSLSI
                     KTSGLLGEQYVALNVGFVIEGETAMMKEGDTFVDTNSAMVLEDLIGQFLYGDKKSDKT
                     EDEKASDSAEPKAE"
     gene            complement(5771..6547)
                     /gene="mlaE"
                     /locus_tag="PXA33_000867"
     CDS             complement(5771..6547)
                     /gene="mlaE"
                     /locus_tag="PXA33_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HDL5120213.1"
                     /translation="MVNFISSIGAVVINFIRTLGRSAFMLWGALIGKPEFRKHTPLLI
                     KQLYVLGVQSLLIIMLSGLFIGMVLGLQGYVVLVDFAAESSLGTLVSLSLLRELGPVV
                     TALLFAGRAGSALTAEIGLMKATEQLSSLEMMAVDPLRRIIAPRFWAGVISMPVLAAI
                     FTAIGIWGGSLVGVDWKGVDGGSFWSVMQNSVNTSDLINGFIKSVIFAFAVVWIALFN
                     GYDCVPTSEGISQATTRTVVNASLVILGLDFILTAIMFGG"
     gene            complement(6540..7343)
                     /gene="mlaF"
                     /locus_tag="PXA33_000868"
     CDS             complement(6540..7343)
                     /gene="mlaF"
                     /locus_tag="PXA33_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HDL5120214.1"
                     /translation="MTKTENLVEVKNLTFKRGERVIYDNLNLQVQKGKVTAIMGPSGI
                     GKTTLLRLIGGQILPESGEILFDGRDICKANNKELYEIRQRMGMLFQSGALFTDISTF
                     DNVAFPIREHTRLPEELIRKLVLLKLEAVGLRGAANLMPSELSGGMARRAALARAIAL
                     DPDLIMYDEPFTGQDPISMGVIVELIKELNQALNLTSIVVSHDVKEVLSIADYAYIVA
                     DKRVIAEGTPEILQASEDPQVVQFLAGKADGPVRFHYPAKDYTEELFNG"
     gene            7568..8962
                     /locus_tag="PXA33_000869"
     CDS             7568..8962
                     /locus_tag="PXA33_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HDL5120215.1"
                     /translation="MNKTMKKSFLTAFILGSTIAAMPAYAEATLPTAVNGQAVPSLAP
                     MLEKVRPAVVSIAVEGKTKGDSRRSREIPEEFEFFFGPHADLFGNRSSAPRNFRGVGS
                     GAVINAEKGYVITNNHVIDNADKITVKLEDGREFKAKLVGADPLSDVALIQVEDPKNL
                     TEIKFADSDKLQVGDFTVAIGNPFGLGQTVTSGIISALGRSTGQSDEGYENYIQTDAA
                     VNQGNSGGPLINLNGELIGINTAIISPSGGNAGIAFAIPSNMANSLVTQIIEFGEVKR
                     GMLGIKGGELNADLAKEFNISTQQGAFVSEVLPDSAAAKAGFKAGDVITEINGQKIRS
                     FSELRAKVATSGVGKEIELTYLRDGKEAKAKVTLQSDSEAKVTASNLIPALKGADFNN
                     YNAKGIKGVEISNVEKGSIAEMRSLKKGDIIIGVNRHSVENIADLRKILETKPSVVAL
                     NIVRDGANFYVILQ"
     gene            complement(9014..9775)
                     /locus_tag="PXA33_000870"
     CDS             complement(9014..9775)
                     /locus_tag="PXA33_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C biogenesis protein"
                     /protein_id="HDL5120216.1"
                     /translation="MKTREQLSQENYQQAVKFAQHFADDVRQEFEREAEQRYQFERTQ
                     FEQSVLQNNAQNRPLAKIASLSCVAILLLAMTYYGLSGRYQVVQSGLQQHQDFQQQMS
                     DAHGTSKNERYILSLQNQLRENPNNGDLWYELGQAYALSNDFDSALVCYDNAEKLLGK
                     RAAILGAIATARYYDNGQKMTVEIQAIIEQALQLDKNENASLLLLASDSFLNNNFQQA
                     LDNWRKVLDSNNDSINRRAIIQSMEMARQMLNSQQ"
     gene            complement(9762..10214)
                     /gene="nrfF"
                     /locus_tag="PXA33_000871"
     CDS             complement(9762..10214)
                     /gene="nrfF"
                     /locus_tag="PXA33_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HDL5120217.1"
                     /translation="MRNSIISIGFLQKIRKILPLVFGLLLFVGTVQAEMVDTFQFQNE
                     ADRVRAISLARSLRCLQCQNQNLVESNATAAYNMRIEVYEMVNQGKSNEEIIRIMTER
                     FGSFVNYDPPFTPQTWVLWGLPVGLFSLLFLAILWRTRKNKGNKNENA"
     gene            complement(10204..10737)
                     /locus_tag="PXA33_000872"
     CDS             complement(10204..10737)
                     /locus_tag="PXA33_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HDL5120218.1"
                     /translation="MKKILLLIPLLLLVVIVGFLTVPLMNKDALAPTEDWQGKPFPEF
                     VGKNLLDSNAHLNNNSLPKEPYILNVWVSWCTWCIKEFPILLELKQKGVPIVGLTYSD
                     RPNDALNALKNWGNPFSLIIDDYEKGFLIQTLKVSSAPSSYLIDKHGVVRYQQKGYNP
                     DFAQDFLPKLEALRNEK"
     gene            complement(10734..12638)
                     /gene="nrfE"
                     /locus_tag="PXA33_000873"
     CDS             complement(10734..12638)
                     /gene="nrfE"
                     /locus_tag="PXA33_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfE"
                     /protein_id="HDL5120219.1"
                     /translation="MLPELGYFSLLLAAISAIFQVGFALLGEVRKQYHWLALAPLFTY
                     LQAAFTTFSFSTLAYAFLTDDFSVIYVAAHSNSQLPDFFKFAATWGGHEGSMLFWLTA
                     LVIWSAVFCKFSQKIDRLFANRTLAMLGLIALGFLLFILLVSSPFERGFPPPPEGRDL
                     NPMLQDIGLIFHPPLLYLGYVGFAVSFAMIVAALVSGGMDAAIMRWLRPWTMISWGFL
                     TAGIILGAWWAYYELGWGGWWFWDPVENASLMPWLLGTALVHSLIVSQQRGIFYYWTI
                     LLAIFAFALSLLGTFIVRSGVLTSVHAFAVDPDRGMALLILFFGLSFIALALFAFRAN
                     LWQGKVRFNLLSKETAFLLINGLFSVAASVVLLGTFYPMIFTVMNWGSISVGAPYFNS
                     VFTPLALLLMIVMGFAVVLGWKQISNKQILVKLWLLPIAVGLALGLIYASISQREHFA
                     LQPLPTIFISLAIWIILTHLPYSQKMWQIRPLAMRLAHIGFAVCVIGAMMNSYYGDEI
                     GVRLKPNQAAELAGYTFKYEDYHDLIGPNYTAEQAVFSISRDEQLLATVTPERRYYDV
                     RTMTMAEVGLFHHNLDDIYIVMGDKFGNLEYAFRLHYKPYVQALWLGGMMMVMAALLA
                     LFGYRRENKK"
     gene            complement(12908..13855)
                     /gene="nrfD"
                     /locus_tag="PXA33_000874"
     CDS             complement(12908..13855)
                     /gene="nrfD"
                     /locus_tag="PXA33_000874"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HDL5120220.1"
                     /translation="MNEYVPFQTPNLVWDGTIAIYLFLLGISSGAVQLAIAYRNSGVK
                     MEKPSQNWIIRSAAILGTLPTIIGLLLLIFHLTKPWTFWKLMFNYNFTSVMSMGVMLF
                     QLYMAVLVVWIAIMFKDWIVRYLPVVSGLIDFAEAKLLKPIEFILFVLAAVLGAYTGF
                     LLSALVSYPMLNNPVLPALFLASGASSGIAATFLVILLAGKLSGESHEVHFMHKFEVP
                     IMVTELGLIVAFFVGLHFGGADKSLALTHALNGFWGNVFWIGVMLIGIAIPLVANIFG
                     SHRLKHNVKFIILISVFDLIGVLCLRYFILYAGQLTVAS"
     gene            complement(13855..14532)
                     /gene="nrfC"
                     /locus_tag="PXA33_000875"
     CDS             complement(13855..14532)
                     /gene="nrfC"
                     /locus_tag="PXA33_000875"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007524349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HDL5120221.1"
                     /translation="MTCTRRDFVSGAGAIAVVASTGLATSITLATEKEQKKIRYAMVH
                     DETACIGCTACMDACRTTNKVPEGVSRLEIIRSEPFGEFPNVQYEFFRQSCQHCTNAP
                     CVSVCPTGASFIDPETGIIDVHSDLCVGCQYCIAVCPYRVRFIHPEKKSADKCNFCRD
                     TNLAEGKQPACVEACPTKALTFGDMNDPSSDIFHKVKNNPVYRTKTALGTEPNLYHIP
                     FGKGEHR"
     gene            complement(14532..15188)
                     /gene="nrfB"
                     /locus_tag="PXA33_000876"
     CDS             complement(14532..15188)
                     /gene="nrfB"
                     /locus_tag="PXA33_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HDL5120222.1"
                     /translation="MKKLISKAGRAVALIQAVVFAGFFAISSANALMPEQPKPLWQSE
                     TADDAKVAQDARRDPNKYCTQCHESANATGKPFHHAGKHFQKDVKSPNNGEPLTCISC
                     HGNISESHRKGAKDVMRFNPHGKASNPSLERSVNEQNQVCFACHTAEKLREKFWAHDT
                     HATKIACTNCHEIHPEKDPMKDIPEKDRIKLCTDCHTKIHSGEFKNPEMKATEAKGTK
                     "
     gene            complement(15207..16709)
                     /gene="nrfA"
                     /locus_tag="PXA33_000877"
     CDS             complement(15207..16709)
                     /gene="nrfA"
                     /locus_tag="PXA33_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HDL5120223.1"
                     /translation="MKFLIKSLAVATISILGCLQTALAEEAKTESLTDKAVKHEKLGV
                     KIESANHLFAEKYPLQYDSWKSTAKSTDRGSALEADPRYVILWAGYAFAKDYNKPRGH
                     FYAVTDVRDILRTGAPKDENDGPQPMACWTCKGPDVPRLIEEKGERGYFDPKWAKYGA
                     EIVNSIGCADCHDTTSEEFKQGKPALRVARPHVLRALNTVGWKFEDLDKHGKRPAVCA
                     NCHVEYYFKNKTDVTFPWDKGVDVDSIEKYYDEINFTDWTHALSKAPMLKTQHPDFEV
                     WSQGTHGKNGVTCIDCHMPKVKDKDGKVYTDHKIGNPFDQFEATCKTCHEQSKETLEK
                     RVKEYKHEVKEAMIRLEDQLVKAHFEAKAAWEAGATQEEMKEILMAIRHAQWRWDYSA
                     AGHGNHFHAPDVMLRTIATGIDRAADARAKLGVVLAKHGVTTPVAIPDISTKEKAQLA
                     IGLDIPKEQAAKDEFLKTVVPQWEKEARAKGLLPAEEADKPVAAPKAEAK"
BASE COUNT         5009 a         3719 c         3328 g         4698 t
ORIGIN      
        1 gatttatgct aaaaataccc aacgagtatt gatgctacaa cgcaaggacg atcctgattt
       61 ttggcaatcg gtgaccggtt cgatcaatat tggagaaaca ccgctggaag cagcagaacg
      121 ggaagtgagc gaagaaaccg gtttgcaaac ttttgagcaa aaacaaccgc ttgttgattg
      181 ccaaaagcag atagaatttg aaattttccc gcattttcgg tataaatacg cacctaatat
      241 cacacattgt gtcgaacatt ggtttttgct gccacttgat agtgagcaag agcctatttt
      301 aaccgaacat ttggcatatc gttgggtttc agtggaagat gcggttcgac tgactaaatc
      361 tcccaacaat gcccaagcaa tacaagaata tttaacatca aaataaggaa acaacagaat
      421 ggcaggccat agtaagtggg caaatattaa acaccgtaaa gcggcacaag atgcacaacg
      481 cggtaaaatt tttactaaat taattcgtga attggtgact gccgccaaac tcggcggtgg
      541 tgatacctct tccaaccctc gtttgcgtgc ggcggtggat aaagcgttat ccagcaatat
      601 gacccgtgat accatcaacc gtgcaatcga gcgtggtgta ggcggtggcg atgataccaa
      661 tatggaaacc aaaatctacg aaggttacgg cccgggtggt acagcagtga tggtggaatg
      721 tttaagtgat aacgctaacc gtacgatttc ccaagttcgt ccaagtttca caaaatgtgg
      781 cggtaaccta ggtactgagg gttcggtagg ttatttattt agcaaaaaag gcttaatttt
      841 agtggcaaat gccgatgaag atgccttaac tgaagctgct attgaggccg gtgcggacga
      901 tattcaacca caagaagatg gtagctttga aatttatacc gcttgggaag atttaggctc
      961 agtgaaagac ggcattgagg ctgcaggctt taaaattgag aatgcagaag taacaatgat
     1021 tccatcaact accgttgaac ttgacgcaga aactgcacca aaacttcttg atttaatcaa
     1081 ccgccttgaa gattgtgatg atgtacaaaa cgtttatcac aatggtgaaa tcagtgacga
     1141 agtcgctgca ttactgtaat aaaaaaatca cgcaaaataa gcataattta ggttatgctt
     1201 attttttatg aacttttttc ctatattact gactaaccta atgtagttta tatgctacta
     1261 ctttactata acattttaag gagagtctat gaaattagtg aaatttgtcc ctgccctagc
     1321 agcaacttta gtattagccg cttgctctaa tgcaaacaca caatctgctc ctcagcaagc
     1381 tcaggacgct gtgactaaag tggctgaaca agcaacacag caagtgaatg aaattgcagg
     1441 taatgcacct cgtgcaagtg gtgcagtaaa agtagaaaaa gcaactgatg gtgcaaaatc
     1501 aattgtatat cgttgccaaa gtggtaaacg tgtaactgcg aattatgtct tccaaggaga
     1561 agaggcaaaa gcagtaaatc tattagtcca aagcggcggt aaaacacaaa agatcccaac
     1621 attaactcgt gatgcctcaa accaagattc tacttcattc aaaacggata aatattcttg
     1681 ggatttagac acaggcttta ccttatcatc taatggccag tcaggtatgc taaccaagta
     1741 tggtgctaaa acagatacca tcgttgctaa attatgtgat gtagataaag cattaacctc
     1801 aaaattaaat aaataatctt tcttgatttt actactcata agcgagaaga taatatcttc
     1861 tcgcttattt tattgcacaa agatggctat tattttagga attgaccccg gctcaagggt
     1921 aacaggttac ggtgtgattc gccaaaacgg gcgacagctg gaatatttag gaagtggggc
     1981 tattcgaacc acggctgaag atctgcctac ccgcttaaaa cggatttatg caggcgtgag
     2041 tgaaatcatc actcaatttc aaccaaatat gttcgccata gagcaggtat ttatggcaaa
     2101 aaaccctgat tctgccttaa agctcgggca ggctcgagga acagcgattg tagcagcggt
     2161 taatcacgat ttacctgtgt ttgaatatgc cgcccgatta gtcaaacaaa ccgttacggg
     2221 aattggttct gctgataaag tacaagtgca agatatggta acccgtatgc ttaaactctc
     2281 cgccaaacca caagccgatg cggctgatgc attagccatt gcgattaccc acgctcattc
     2341 aatccaacat tcactgattg ttgctaagca aagccagcag aatgtgagta gtgagaaaga
     2401 gcaaatttta gcactaatga aaacccgtta tagccgggga aggtttaggt taaaaggcta
     2461 actcaatata aaatgaccgc ttggaactaa tgtttcaagc ggtcattttt tgagaaaatt
     2521 ttacaattac tcgccttcaa aaggagctga gaaacgttca attctcgccc caatctgacg
     2581 taatttttct tcgatgtgtt catagccacg gtctatgtgg taaattctat ccacaatcgt
     2641 ttcacccgtt gcaatacagc ctgccaacac taagctgatg gaagcacgca agtcagttgc
     2701 cattacttct gctgatttta actgctctac accatagcaa attgcaatat taccttcaat
     2761 ttccgctttg gcacccatac gaatcagctc aggaatatgc ataaaacggt tttcaaaaat
     2821 cgtttctgta atacggcttg tgccttctgc aactgtattt aataaggtaa attgagcttg
     2881 catatcagtt gggaaacctg ggtgtggcat agtgcgaatg ttcactgctt tcgggcgacg
     2941 acctagagaa tcaagcgtga tagtattttc cgtcacatca acctgcattc cagcttcacg
     3001 taatttttcg atcactgcat caagagtgtc tgctttggtg ttacggcaag taattcgacc
     3061 accggaaata gccgctgcca ctaaaaatgt accggtttca atacgatccg gcactacgct
     3121 gtgttcgcaa ccgcctaaat gctcaacccc ttcaatggta atcatatcgg tacctgcacc
     3181 agaaatttta gctcccatcg catttaagaa atcggcagta tctacaattt caggctcacg
     3241 agcagcattt tcaataacag tagtaccttt tgctagagta cctgccatca tcaccgataa
     3301 agttgctcct acgctcactt tatccatata gattcttgca ccgtttaaac gaccgtttga
     3361 agttgctttg acatagcctt catcaagctc gattttcgcc cccatttttt caagaccgga
     3421 aatgtgcata tctaccggtc ttgccccgat agtacaaccg cccggtaaag aaacttgccc
     3481 aagttggaac cgggcaacta atggagctaa cgcccaaatt gaagcacgca ttgttttcac
     3541 taattcgtaa ggtgcaacgt aattattgat cttactcgca tcaatatgta ccacgccttt
     3601 atcgctacct ttttcaaccg ttacccctaa ttggcgtaag attttaaaag tggtgtccac
     3661 atcttttaaa tcaggcacat tttttaacgt aactgttttt tcagccaaaa tagcggcaaa
     3721 taaaataggg agtgcggcat ttttcgcacc tgaaatatcg acagtaccat ttaaagaaaa
     3781 tggaccatac acacgaaatt tttccatttg ggagatccta aaaaataata atttgtaggg
     3841 gcgaaaaatt tttcgcccaa ttttccaacg tttacaaaag agtttgggcg aatagtgatt
     3901 cgcccctaca tttcatctat ccaaccatat ttagcaaacg ctcagttttc catttttcca
     3961 cgctgaatac tttaatggtt aaagcgtgaa tcgcattagt agtgaaatac tcagctaaag
     4021 gggcataaac agcttgctgt tgttttactt ttgaaagaga agcaatgctg tcacttacta
     4081 caataacgcc aaagtgggca ttctcgcctt gagcgtggac ttctgttgca tcagataatg
     4141 cttgttttaa aatttcttca atttgtgatg gattcattct gacttcctaa aatatgattg
     4201 gtaactcact caaataataa attgctcaaa ccaaccgtct aaatcgtata atgcagccaa
     4261 agttctgaca gaatctggcg tattaatcaa actgtttggt atgattttat ggtaatgatt
     4321 cagtaattca gcaagtaagg taaaaccggc agaatcaatc cgagtgattt cttttaaatc
     4381 ccaatatagg ctttgtttcc ctgttggaga taaaaaagaa gcacgttgct tccataaagg
     4441 gagcaacgtg ttacgagtta attcgcctga tagtttcaca aaaagacttt cgttattttg
     4501 ctgaatgtcc cattgtaatg ttttttgagg cataactaaa ctacttattt taaagtgata
     4561 ggctgttttg cagattgtgc cacttgtgca attaaggcat caatcccttt ttgacgaatc
     4621 acacccgccc attcgttttg cttggttgca accatactta ctccttcagc ggccatatcg
     4681 tatgcttgcc attcacctgt tttgctgttt ttacgccatt taaaatctaa tttgatcggc
     4741 tgtgcagcac ctttacctga cacgtttaca cgaatgctta cgatagattt accatccacc
     4801 gatttcgggc tttcaatttg cacactttga tcttgatact gggttaacac ctgagcataa
     4861 gactgctcaa caaattgacc aaaagcatta aagaatgcct ctttttgtgc atcagtcgca
     4921 gccgataagt ttttccctaa cactaattgg cctgcatatt tcacgtgaac gtgtggcatt
     4981 aaatcgttac gcacaatggt acgcaaatat tcagggtttg atttaatttt cgcttgattt
     5041 gctttaatat cgccaaataa tttatctgcg gtttgttgca ttaatacata agggctagtt
     5101 tcagcaaatg cggtggttga aaataatgca gaaactgcca ctaagcctgt tacgataatt
     5161 tttttaatgt tttttaacat tattgatgct ctccaaaact ctaaattaga ggttgttgta
     5221 cttaattact ctgcttttgg ttcagcagaa tcacttgctt tttcatcttc agttttatct
     5281 gactttttat caccatataa gaactgaccg attaaatctt ctaacaccat tgcagaattg
     5341 gtatctacaa aagtatcacc ttctttcatc attgcagttt caccttcgat tacgaagcca
     5401 acatttaacg caacgtattg ctcgcctaat aagcctgagg ttttaattga gagcgagctg
     5461 gtatcgggaa tttgattaaa actttcattt accgctaatg ccactttcgg ggtgtaggtt
     5521 ttgttatcaa gtgaaatatc agccactcgg ccaactacca caccgccaac tttaataggc
     5581 gcacgaacct ttagtccacc aatattatca aaagttgcat acagggtgta agttttctca
     5641 tttgagaagc cctgcacatt tgctactctt aagcctaaaa agacaagagc tgcaagccca
     5701 agtagtacaa ataagcctac ccaaaattca tatttaattg attgacgcat tgtctgttcc
     5761 tttcaaaaaa tcaaccgccg aacattatcg cagttaaaat aaaatccaat cctaaaatca
     5821 ccagtgaagc atttaccacc gttcttgtgg tcgcttggct gattccttct gaagtcggta
     5881 cgcaatcgta gccgttaaat aaggcaatcc agaccaccgc aaaggcaaaa atcacacttt
     5941 taataaagcc gttaattaaa tcgctggtat ttaccgagtt ttgcattacc gaccaaaaac
     6001 tgccaccatc aacgcctttc caatctaccc cgactaatga gccgccccaa attccgatag
     6061 cggtaaaaat agcagctaac accggcattg aaatcacgcc tgcccaaaag cgaggagcga
     6121 tgatacgtct aagcggatca accgccatca tttcaagact ggaaagttgc tctgtggctt
     6181 tcatcagccc gatttctgcg gtcaatgccg aaccggctcg ccctgcaaat aataatgcag
     6241 taactacagg accaagttct cgtaaaagcg agagtgaaac caaagttccc aagctacttt
     6301 cggcggcaaa atcgaccaat accacatagc cttgtaagcc taacaccatt ccgataaata
     6361 agcccgacag cataataatt aagagcgatt gcactcctaa cacataaagc tgtttaatca
     6421 gtaacggggt atgttttcta aattcaggtt taccgattaa tgctccccat aacataaaag
     6481 cggaacgccc taatgtacgg ataaaattaa tcaccaccgc cccgatagag ctaataaaat
     6541 taaccattaa atagctcctc cgtataatct tttgctggat aatggaaccg caccggacca
     6601 tcagctttac cggctaagaa ttgtaccact tgcggatctt cgcttgcctg taaaatttcc
     6661 ggtgtacctt cagcaatcac tcgtttatct gccacaatat aagcataatc ggcaatactt
     6721 aatacttctt tcacatcgtg agacaccaca atggaggtca aattcaatgc ctgatttaac
     6781 tctttaatca gctcgacaat cacacccata ctaatcggat cttgcccggt aaacggctca
     6841 tcatacataa tcagatccgg atctaacgca atcgctcgag caagggcggc acgtcttgcc
     6901 ataccgccgg aaagttcgga cggcattaaa tttgccgcac ctcgcaaccc aaccgcctcg
     6961 agcttcaata acaccaattt gcgaattagc tcttcgggta aacgggtatg ctcccgaatc
     7021 gggaaagcca cattatcgaa ggtagaaata tcggtaaata acgcccccga ttgaaacaac
     7081 atccccattc gttggcgaat ttcatacaac tctttgttat tggctttgca aatatctctg
     7141 ccatcaaata aaatttcgcc cgattcaggt aaaatctgcc caccgattag cctcaacagg
     7201 gtggttttac caatcccaga aggccccata atggcagtca ctttaccttt ctgcacttgc
     7261 agattcaggt tatcgtaaat cactcgctcc cctcgcttga aggtcaagtt ttttacttca
     7321 accaaatttt ccgtttttgt catcaaatag gactcaaatt aataaaagtg atatttatgg
     7381 tctatcaaat ttgccagaat gtcaaaaaac ttttaataat ctgacaaaaa tctgccttta
     7441 caagcggtca gatttgctaa attttttgca aaccggctga actgaaaaag aaaggtcttg
     7501 tcttagccaa tacgagcgtt ttcactcgta gattataaaa acataggata tgaggaacat
     7561 tcacaatatg aacaaaacaa tgaaaaaatc atttttaact gcattcattt taggctcaac
     7621 cattgccgca atgccggcgt atgcagaagc aacattaccc acagccgtaa atgggcaagc
     7681 tgtgccaagc cttgcaccaa tgctggaaaa agttcgcccg gcggtggtga gtattgcagt
     7741 agaaggtaaa accaaaggcg atagccgccg cagtcgtgaa attccggaag aatttgaatt
     7801 tttcttcggt cctcacgccg atttattcgg caaccgctca tcagctccac gcaatttccg
     7861 tggcgttgga tcaggtgcag tcatcaatgc cgaaaaaggt tatgtgatta ccaataacca
     7921 cgtgatcgac aatgccgata aaattaccgt aaaacttgaa gacggacgtg aatttaaagc
     7981 taaattagtt ggtgctgatc ccctttcaga tgtggcgtta attcaagttg aagatccaaa
     8041 aaacttaact gaaattaaat tcgccgattc cgataaatta caagtgggcg attttacagt
     8101 tgcaatcggt aacccgtttg gcttaggaca aactgttact tcaggcatca tctcggcact
     8161 tggtcgttca accggtcaat ccgatgaagg ctatgaaaac tatattcaaa ccgatgcggc
     8221 agttaaccaa ggtaactctg gcggcccgtt aattaactta aacggcgaat taatcggaat
     8281 taacaccgct attatttctc caagtggtgg caatgccggt atcgcctttg cgattccaag
     8341 taatatggca aacagcttgg tgactcaaat tattgagttt ggtgaagtaa aacgtggaat
     8401 gttaggcatt aaaggcggtg aattgaatgc tgaccttgct aaagagttca atatttccac
     8461 ccaacaaggg gcatttgtaa gcgaagtctt accggattct gctgctgcta aagccggctt
     8521 taaagcaggt gatgtgatta ctgaaatcaa cggtcaaaaa atccgtagtt tcagcgaatt
     8581 acgagcaaaa gtagctactt caggtgtagg taaagagatt gaacttactt acctgcgtga
     8641 cggcaaagaa gccaaagcaa aagtgacctt acaatcggat tctgaagcca aagttactgc
     8701 ttcaaacctc attccggcat taaaaggggc agattttaac aactacaatg ccaaaggcat
     8761 taaaggggtt gaaatttcta acgtagaaaa aggctcgatt gctgaaatgc gaagcctcaa
     8821 aaaaggcgat attatcatcg gcgtgaaccg ccactcggta gaaaacattg ccgatttacg
     8881 caaaatatta gaaaccaagc catctgtggt tgcactcaat attgtgcgtg acggtgcaaa
     8941 tttctatgtg attttgcaat aaaccttttg atccactcaa acaaaagccg atgacaaaaa
     9001 tcatcggctt ttgttattgc tgggaattta acatctgtcg tgccatttcc atactttgga
     9061 taatcgctct gcggttgata gaatcattat tgctatctaa cactttacgc caattatcga
     9121 gagcttgttg aaaattgtta tttaaaaagc tgtcggaggc tagcaggagt aaactggcat
     9181 tttcattttt atccaactgt agggcttgct cgataatggc ttgaatttca acggtcattt
     9241 tctgcccgtt atcgtaataa cgagcggtgg caatcgcccc caaaatagcc gctcgttttc
     9301 cgagtagctt ctcggcatta tcgtagcaaa ccagtgccga atcaaaatca ttgcttaacg
     9361 cataagcctg ccctagttcg taccataaat caccgttatt cgggttttct cgaagctggt
     9421 tttgcagact caaaatatag cgttcatttt tcgatgtgcc gtgtgcatcg ctcatctgtt
     9481 gttgaaaatc ttgatgttgt tgcaaacctg attgcaccac ttgataacgt ccggaaagcc
     9541 catagtaagt cattgctaac aacaaaatcg ccacgcaact caggcttgcg attttagcaa
     9601 gcggtcgatt ttgagcatta ttttgcaaaa cgctctgttc aaattgcgtt cgttcaaatt
     9661 ggtaacgttg ctctgcctca cgctcaaact cttgtctgac gtcatctgca aaatgttggg
     9721 caaatttaac cgcttgttgg tagttttctt ggcttaattg ctcacgcgtt ttcatttttg
     9781 ttgcctttat ttttgcgagt tcgccacaaa atagctagaa ataacaggct aaacaagcca
     9841 accggcaagc cccataatac ccatgtctgt ggcgtgaatg gtggatcgta attcacaaaa
     9901 ctgccgaaac gctcggtcat aatgcggata atttcctcat tacttttccc ttgattcacc
     9961 atttcataca cttcaattcg catattgtaa gccgccgtcg cattggattc gaccaaattt
    10021 tggttctgac attgcaaaca tcgcaacgaa cgagccagtg aaatcgcccg tacccgatcc
    10081 gcctcatttt gaaattgaaa ggtgtccacc atttcagctt gaaccgtgcc gacaaatagc
    10141 aacaagccaa atacaagcgg taatattttg cggatttttt gcaaaaagcc gatggaaatt
    10201 atgctatttc tcatttctga gagcctctaa tttgggtagg aaatcctgtg caaaatcagg
    10261 gttgtaaccc ttttgttggt aacgcactac accgtgttta tcaatcaaat aactggaagg
    10321 tgcagaagag actttcaggg tttggatcaa aaagcctttt tcgtaatcat caataattaa
    10381 actaaacgga tttccccagt ttttcaatgc gttgagggca tcatttgggc ggtcgctata
    10441 agttaagccg acaatcggca cgcctttttg ttttagttcc aacaaaatcg ggaactcttt
    10501 aatgcaccaa gtacaccagc tcacccacac attgagaata taaggctctt tcggcaagct
    10561 gttgttattt aaatgcgcgt tactatcaag taagttcttg cctacaaact ctggaaacgg
    10621 cttgccctgc caatcttcag tgggagcaag ggcatcttta ttcattaacg gcacagtcaa
    10681 aaagccaaca ataactacaa gtaacaggag cggaataagt aacaggattt ttttcatttt
    10741 ttgttttctc tacgataacc gaataatgcc aacagggcgg ccattaccat catcatacca
    10801 cctaaccata aggcttgcac ataaggctta tagtgcaaac ggaaggcata ttctaaattg
    10861 ccgaacttat cccccatcac aatataaata tcgtctaaat tgtggtggaa aagccctact
    10921 tctgccattg tcatcgttct tacatcataa tagcgtcttt ccggtgtgac ggttgccagc
    10981 aactgttcat ctcggctgat agaaaaaacg gcttgctcgg cggtgtagtt cggaccgatg
    11041 agatcgtgat aatcttcata tttaaaggta taaccggcaa gttccgctgc ttgattcggc
    11101 tttaaccgca cgccgatttc atcgccgtaa taactgttca tcattgcccc aatcacgcaa
    11161 accgcaaagc cgatatgggc taaccgcatc gcaagcggtc gaatttgcca cattttttgc
    11221 gaatagggca aatgagtgag aataatccag atcgcaaggc tgataaatat ggtgggtaac
    11281 ggctgcaaag caaaatgctc tctttgcgag atggaggcat aaatcaaccc taacgctaaa
    11341 ccgactgcaa tcggcagcaa ccaaagtttt accaatatct gcttatttga aatctgtttc
    11401 cagcccagca ccaccgcaaa gcccattact atcattaaca gtaacgctag tggcgtgaat
    11461 acgctattga aatagggggc ccccactgaa atcgatcccc aattcatcac tgtgaagatc
    11521 atcgggtaga aagtccccag caataccaca gaagcagcca cactaaataa gccgttaatc
    11581 aataaaaatg ccgtttcttt agaaagtaag ttgaaacgca ctttgccttg ccagagattc
    11641 gctcggaatg caaacagagc aagggcaatg aaacttaagc cgaagaataa gatcagcagt
    11701 gccattcctc tgtcgggatc gaccgcaaag gcatgaaccg aggtcagtac acccgaacga
    11761 acaataaacg tgcctaatag gcttaaagca aaggcgaaaa tggcaagtag aatcgtccaa
    11821 taatagaaaa tgccacgctg ttggctcaca attaaactat gcaccaatgc ggtgccaagt
    11881 agccaaggca ttagggaggc attttccacc ggatcccaaa accaccagcc accccagccg
    11941 agttcgtaat atgcccacca cgcccctaaa ataatgccgg cggtgagaaa tccccacgaa
    12001 atcatcgtcc acggacgaag ccaacgcata atcgcagcgt ccattccgcc tgaaacgagg
    12061 gcggcgacaa tcattgcaaa gctcaccgca aagccgacat agcccaagta gagcaaaggc
    12121 gggtgaaaga tgagcccaat atcttgcaac attgggttga gatctcgccc ttcaggtggt
    12181 ggtggaaagc cacgttcaaa tgggctggaa accaatagaa tgaaaagtag aaaacctagt
    12241 gcaattagcc ctaacatcgc taaggttcgg ttggcaaaca agcggtcaat tttttgcgaa
    12301 aatttgcaaa ataccgcaga ccaaatcaca agagcggtga gccaaaacag cattgagcct
    12361 tcgtgtccgc cccaagtggc ggcaaattta aaaaaatcgg ggagctggct gttggaatga
    12421 gcggcaacgt agataaccga aaaatcatcg gttagaaaag cataagctaa ggtagaaaag
    12481 gaaaatgtgg taaaagcagc ttgcaaataa gtgaaaagcg gagccaatgc cagccaatga
    12541 tattgcttgc gaacttcgcc tagaagtgca aaaccaactt gaaaaatggc actaattgca
    12601 gccaataaaa gtgaaaaata gccgagttcc ggtagcataa aaaatccaaa aaatgaccgc
    12661 ttgtgaatgt aaaaaaagcc ctctccactt gtgggagagg gacagatttt tcttcgttca
    12721 gaagaaaaat cagggagagg ggaattaaat aattttgcta aaaataacaa tactccctct
    12781 ctctgaaaaa tatttctgaa cgaaatattt ttcggtctca atactcgcta cgctcgctcc
    12841 ttcggagccg ttggcaaagc caacgttcaa atgtaaacat ttgtcccaca aggggagaga
    12901 gtatatccta acttgcaacc gttagttgcc ctgcataaag gataaagtaa cgcaagcaga
    12961 gtacaccgat taagtcgaaa acggaaatta agataataaa tttcacgtta tgtttcaagc
    13021 ggtggctacc aaaaatattg gcaacaagcg gaatcgcaat accgattaac attacgccta
    13081 tccagaacac attcccccag aagccgttaa gtgcatgggt taaagcgagc gatttatctg
    13141 ctccaccgaa gtgtaagccc acaaagaacg ctacaattaa gcctaattcg gttaccataa
    13201 tcggcacttc aaatttgtgc ataaagtgga cttcgtggct ttcaccggat aacttgcctg
    13261 cgagtaaaat cactaggaaa gttgctgcaa tacctgacga tgcccctgat gccaagaaca
    13321 atgccggtaa cacagggtta ttcagcattg gataactcac taacgccgag agtaagaaac
    13381 cggtgtaagc accaagtaca gcagccaata cgaataagat aaattcaatt ggttttaaca
    13441 gcttcgcttc ggcaaaatcg atcaaaccag agaccacagg tagataccga acaatccaat
    13501 ctttaaacat tatggcgatc caaaccacca atactgccat ataaagttgg aataacatta
    13561 cccccatcga cattactgag gtaaagttat agttgaacat taatttccag aatgtccaag
    13621 gtttggttaa gtggaaaatc agtaacagta agccgataat ggttggtaat gtgccaagaa
    13681 tagccgcact acgaataatc cagttttggc ttggtttttc cattttgaca ccgctattac
    13741 ggtaagcaat cgctaactga accgcacctg atgaaatacc aagtaggaat aagtaaatag
    13801 cgatagtgcc atcccaaaca aggtttgggg tttggaaagg tacatattca ttcattatct
    13861 atgttctcct ttaccaaatg gaatgtgata taaattcggc tccgtgccta atgccgtttt
    13921 ggtgcgataa accggattat ttttcacttt atggaaaata tcgctacttg gatcattcat
    13981 atcaccaaag gttaaagctt tagtcggaca agcctctaca caagcaggct gtttgccttc
    14041 tgcaagattg gtatctcggc aaaagttaca tttatccgcc gattttttct cagggtgaat
    14101 aaaacgtaca cgatacgggc aaaccgcaat acagtattgg caaccgacac acaaatctga
    14161 gtgtacatca ataatcccgg tttcaggatc gataaatgaa gcaccggtcg ggcaaacact
    14221 cacacaaggt gcattggtac agtgttggca agattggcgg aaaaactcat attgtacgtt
    14281 cggaaactca ccgaaaggct cactacggat aatttctaaa cgagaaaccc cttccggcac
    14341 tttgttggtg gtgcgacaag cgtccataca agccgtacag ccgatacagg cagtttcatc
    14401 atgaaccatc gcatagcgga ttttcttttg ttctttctca gtagcaagag ttatggaggt
    14461 ggcaagccct gtacttgcca caactgcgat agcacctgcc cctgagacga aatctctacg
    14521 ggtgcaagtc attattttgt tccttttgct tctgtggctt tcatttcagg atttttaaat
    14581 tcgcctgaat gaattttggt atgacaatcc gtacaaagtt taatgcgatc tttttcagga
    14641 atgtctttca tcggatcttt ttccgggtga atttcatgac agttggtaca agcaatttta
    14701 gttgcgtgcg tgtcgtgagc ccagaatttt tcacgcaatt tctctgccgt atggcaagca
    14761 aaacagactt gattttgctc atttaccgaa cgttcaagtg atgggttact tgctttaccg
    14821 tgtgggttaa aacgcattac gtcttttgcc cctttgcggt ggctttcaga aatattaccg
    14881 tggcaactta tacaagtgag tggctctcca ttattcgggc ttttcacatc tttttggaaa
    14941 tgtttgcctg cgtgatggaa aggcttacct gttgcgtttg ccgattcgtg acattgagta
    15001 caatatttat ttggatcacg gcgtgcatct tgagctactt ttgcatcatc agccgtttct
    15061 gattgccaaa gaggttttgg ctgctcaggc attaacgcat tcgcactgga aattgcaaaa
    15121 aaacctgcaa atacgaccgc ttgtatgagg gcgactgctc gtcctgcttt tgagattaat
    15181 tttttcatag ttcacctcaa gctacattat tttgcttctg cttttggtgc tgctaccggt
    15241 ttgtctgctt cttctgcggg taataaacct ttagcacgag cttctttttc ccattgagga
    15301 accacagttt ttaagaactc atcttttgct gcttgctctt taggaatatc taaaccaata
    15361 gcaagttgtg ctttctcttt agttgagatg tccggaatcg ccactggcgt tgttacaccg
    15421 tgtttcgcta acactacacc taatttagca cgggcatctg ctgcacggtc gatacctgtt
    15481 gcgatggtac gtaacattac atcaggtgcg tggaagtggt taccgtgacc tgccgcagag
    15541 taatcccaac gccattgagc gtgacgaatt gccattaaga tctctttcat ctcttcttga
    15601 gttgctccag cttcccaagc agctttcgct tcaaagtgag ctttcactaa ttgatcttct
    15661 aagcgaatca ttgcctcttt tacttcgtgt ttatactctt ttacacgttt ttccaatgtt
    15721 tctttgcttt gttcatgaca agttttacaa gtcgcttcaa attggtcaaa cggattaccg
    15781 attttgtggt cagtataaac tttgccgtct ttgtctttca cttttggcat atggcaatca
    15841 atacaggtta caccattttt cccatgtgta ccttgtgacc aaacttcaaa atctgggtgc
    15901 tgcgttttta gcatcggagc tttcgataaa gcatgagtcc agtctgtaaa attgatttca
    15961 tcgtagtatt tttcaatgct atctacatct acacctttat cccaagggaa agttacatct
    16021 gttttatttt tgaaatagta ctccacatgg cagttagcgc acacagcagg gcgtttacca
    16081 tgtttatcta aatcctcaaa cttccaacca accgtattca atgcacgtaa tacgtgagga
    16141 cgtgcaacac gtagtgcagg tttaccttgt ttaaactctt ctgatgtcgt gtcatggcag
    16201 tctgcacaac ctattgagtt tacaatttca gcaccatatt tcgcccattt aggatcaaaa
    16261 tatcctcgct cacctttctc ctcaattaag cgtggaacat cagggccttt acatgtccaa
    16321 caagccatag gttgtggacc atcattttca tctttaggcg cgccggtgcg caaaatatca
    16381 cgcacatcgg taactgcata aaagtgacct cgtggtttat tataatcttt cgcaaaagca
    16441 tagcctgccc acaaaataac ataacgaggg tcagcctcta acgcacttcc tcgatctgtg
    16501 gattttgcag ttgacttcca agaatcgtat tgtagcggat atttttctgc aaataggtgg
    16561 tttgcagact caattttcac gcctaatttt tcatgcttaa ctgctttatc tgttaatgat
    16621 tcagttttcg cctcttcagc taaagcagtt tgtaaacacc ccaatattga aattgttgcc
    16681 acagctaggc tttttattaa aaatttcatt ttggctctcc caatcaggta aaaataatta
    16741 gagaaaaata agaa
//
LOCUS       DANLRK010000037        16743 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.037, whole genome shotgun sequence.
ACCESSION   DANLRK010000037 DANLRK010000000
VERSION     DANLRK010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 16743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16743
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.037"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            <1..689
                     /locus_tag="PXA33_000878"
     CDS             <1..689
                     /locus_tag="PXA33_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HDL5120224.1"
                     /translation="WKYAFKLITPIFMGYFAAGVAYGMLATNAGMPAWFTIVMSFTVV
                     SGTAQYAAIPFFVSGVGAVSVFLSTLLMSLRFSFYTLNMMEHKPISKAKSLVAIAGLT
                     DEGFAVLSSLPKEQKQALFFKIAVLCVAYWTFSTIVGVLLGDSVANYIPHLDFALPCL
                     FAILAYEQYKNQKQWKPIVIALIGFLLARQITETSVLLVAILVAIVIVALLPQSSSIK
                     LEKGVENEHK"
     gene            700..1002
                     /locus_tag="PXA33_000879"
     CDS             700..1002
                     /locus_tag="PXA33_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HDL5120225.1"
                     /translation="MFAALVLGSQICRFLPEFLPKKVLSSPILQKLNKMLPLVIMLLL
                     LLTSLNFPKNGEGFNLILAQILALIGVLGSYVWLKNTFLSVALGIFGVNVFLSLLG"
     gene            complement(1123..2424)
                     /locus_tag="PXA33_000880"
     CDS             complement(1123..2424)
                     /locus_tag="PXA33_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDL5120226.1"
                     /translation="MSLFKFEERGSNARQEIIAGLTTFLAMVYSVIVVPGMLSQAGFP
                     AESVFIATCLVSGLGSILIGLWANAPMAIGCAISLTAFTAFSLVLGQQVSIPVALGAI
                     FLMGVVFTLISATGIRTWILRNLPSSIAHGAGIGIGLFLLIIAATNVKLVVNSGMDIP
                     VKLGDFTSFPVLVSLIGLAAIIGLEKLKVKGSILWVIIAITIVGLIFDPNVKYTGFFK
                     MPSFGENSQFLNLDIMGALNTAILPVVFALVMTAIFDATGTIRAVAGQAGLLDKDGQI
                     INGDKALTSDSFGSILSGLFGTAPAAVYIESAAGTAVGGKTGLTAVVVGIGFLFMLFF
                     QPLAFLVPSYATAPALMYVGLLMLSNVSKLDFSDFVGAMSGLVCAVFIVLTANIVTGI
                     MLGFATLVIGRLISGEANKLNLGTVIIAIILVAFYAFDLAI"
     gene            complement(2557..3531)
                     /gene="secF"
                     /locus_tag="PXA33_000881"
     CDS             complement(2557..3531)
                     /gene="secF"
                     /locus_tag="PXA33_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HDL5120227.1"
                     /translation="MAIENVQQDVAEIKLPYKLIPFMKYRYVGFIFSLIVTALCIFTI
                     ATKGFNWGLDFTGGTVIETQFSQPADLAKLRTELENSGYGNALVQTTGSQKDLMIRLA
                     ASAGDMSVGNKVMEMIHQKLDPNATIKSIEFVGPNVGEELTQGAIYATLATLAMLLIY
                     VGTRFEWRLAVGGIVALFHDVLVTIGIFSFLQIEIDLTFVAAILSVVGYSLNDSIVVF
                     DRVRENFSKVRRSSSIEIIDISLSQTLSRTLMTSVTTLFVVLALFYLGGPTLHSFSLA
                     LLIGIGFGTYSSIYIAIGVALQLGLKREHMVQPKVEKEGADQDAFIDY"
     gene            complement(3541..5382)
                     /gene="secD"
                     /locus_tag="PXA33_000882"
     CDS             complement(3541..5382)
                     /gene="secD"
                     /locus_tag="PXA33_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HDL5120228.1"
                     /translation="MLNRFPLWKNLMVIFVIAIGALYALPNLYGDDPSVQISGTRGQQ
                     ATPETLSQVQSTLSAMNIQPKSAVLENGSILVRLESSEQQLPAKEKISEALGNNYSVA
                     LNLAPATPQWLSDIGGSPMKRGLDLLGGVRFLMEVDMNTALAKQQDTLQDSLRSDFRK
                     EKLQYQAVKKGENFATEVEFADNDTAEKAVRLVRLSHPTLDILVNDNKVTLTPSAQAL
                     SESRTMAIEQNLSILRKRVEELGVSEPTIQRQGADRIVVELPGVQDTARAKELLGATA
                     TLEFRLVNTEANVASAAKGIVPADSGLQNTRDGEPVVLRRKPSLGGEHIIDATSGTDE
                     RGLPQVSIRLDTEGGEMMGEITKMAIQKPMATLYTEFKDSGRKDANGKVILEKHQEVI
                     NVATIQTRLGNQFQITGINSPAEAQNLAVLLRSGALIAPIVIVEERTIGASLGADNVK
                     QGMEASILGLGLTILFCLAFYKVFGIFASLALIVNMILTIGLMSLIGATLTMPGIAGI
                     VLAVGMSVDANVLIYERIKEEIRNGRPIQQAIHEGYNGAFSSIFDSNLTTILTSLVLY
                     GVGTGPVKGFAVTLALGVIISMFTAITGTRMLVNWVYGGKRVKKLWI"
     gene            complement(5422..5715)
                     /gene="yajC"
                     /locus_tag="PXA33_000883"
     CDS             complement(5422..5715)
                     /gene="yajC"
                     /locus_tag="PXA33_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005713354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HDL5120229.1"
                     /translation="MQQGGGMEMIFILIIFGAIFYFMIYRPQAKRQKQQRDLLASLTK
                     GDEVLTSGGLIGKITKVTADNDNIVIALNDTTEVTIKRDFVVAVLPKGSLKSL"
     gene            5916..6473
                     /locus_tag="PXA33_000884"
     CDS             5916..6473
                     /locus_tag="PXA33_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HDL5120230.1"
                     /translation="MKKLFAQAVIFGAFFASSAFAHNVQLNQALPAVSVAKDGELAVA
                     GGKVSYKNWHSSSLAGKVRVIHHFAGRSSVKEKNEDLMTAIKNAGFDRSKYQTVTIVN
                     ADDAIVGTGVFVKNSVENGKLDNPHSQVVLDQQGSVKKAWGLKEKESFIAVLDKSGKV
                     QFTSEGKLSAKQIQEVIGLVKTLTQ"
     gene            complement(6514..7482)
                     /locus_tag="PXA33_000885"
     CDS             complement(6514..7482)
                     /locus_tag="PXA33_000885"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HDL5120231.1"
                     /translation="MKLFTLENAFLRITLSDVGASWLSCIVKMPNEEREVLVTTSAKN
                     WQKQTAFFGATIGRYANRIANAEYRLNGKTYQLAKNNGEHNLHGGELGADKIVWQLES
                     QSEQAVKFSYFFADGEEGFGGEVKAFVEYRLTENELQISFDAVANQDTPLCLTNHAYF
                     NLQGSETILDHELIINADGYLPVDASGIPNAPLKKVESTSFDFRNAKKIGQDLLADDD
                     QKAVKGYDHSFHLAKNSENPTACLAVEDLKMELRTSMPALQLYTGNWLKGQPNLTKGE
                     YEDYAGVALEPEFFPNSLNQPELLQFGGITKAGEEYHHTISYRFII"
     gene            complement(7608..8771)
                     /gene="galK"
                     /locus_tag="PXA33_000886"
     CDS             complement(7608..8771)
                     /gene="galK"
                     /locus_tag="PXA33_000886"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HDL5120232.1"
                     /translation="MKPQEIALQQFEQHFGYKAERTVFAPGRVNIIGEHTDYNDGFVM
                     PCAINYGTAVSFAKRSDNKWRVFAIDLNQQDEFDLSQAIEPSEQKWANYVRGVVKFIQ
                     AKHPEFKQGADLAITSDVPMSSGLSSSAALEVSIGKTAQLLGDLPLSLPQIALIGQEA
                     ENKFVGCNCGNMDQLTSALGQQDHLVMIDCRSLEITPTPVPQGYSIAIINSNVKHDLV
                     TGEYNSRREQCEFAAHFFGKAKLRDVTAEQFIERASELSEENELAYKRAKHIISENQR
                     VLEAVEALKANDMVKLGELMAQSHDSMRDDFEITCPEVDCLAEIAQQAIGKNGGARMT
                     GGGFGGCVVCLVPNDKVEDLRQAVANNYEKQTGIKETFHVCTASDGVRECPAR"
     gene            complement(8781..9827)
                     /gene="galT"
                     /locus_tag="PXA33_000887"
     CDS             complement(8781..9827)
                     /gene="galT"
                     /locus_tag="PXA33_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HDL5120233.1"
                     /translation="MSEFVLNDHPHRRFNPLKNQWILVSPHRAKRPWQGQQEEIVQDE
                     KPSYDPSCYLCPGNKRITGEQNPNYTKPFVFKNDFSALLPDTPAPIPNDDPLFQISHT
                     QGESRVICFSPDHSKTLPQLSIEEITDVVTVWQEQANELKEHYQWVQIFENKGSMMGC
                     SNPHPHGQIWASNFLPNEIAIEDECQAKYFAQYGSPLLLDYAQRELDKQERIVAETED
                     WIAVVPYWAAWPFETLLLPKAKQFKSITDLNEAEKADLALALKKLTTRYDNLFNISFP
                     YSMGFHFAPFNGKENAHWQLHAHFYPPLLRSATVRKFMVGYEMMAESQRDLTPEQAAE
                     RLNAVSDTKHYKDK"
     gene            10040..10891
                     /locus_tag="PXA33_000888"
     CDS             10040..10891
                     /locus_tag="PXA33_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF817 domain-containing protein"
                     /protein_id="HDL5120234.1"
                     /translation="MERLNKFDAWLMNHKPNARLSGASRLLTEFWFFGLKEARACLFA
                     GLFFIAMFSVPKTGLWGLPRYDILLIFAIVVQIAMVKLGLETWDELKAISLFHLVGFV
                     LEVFKTSSNIQSWAYPDFAYTKLFNVPLFTGFMYAAVGSYIIQAWRLFDLKIKSHLPY
                     WLATLTALMIYLNFFTHHYIGDYRWYIAAFALGLYARTFVFFTPYDITRKMPLLLSFF
                     LIGFFIWLAENIGTFAGIWRYPNQFGAWSMVHMSKWSAWALLVMMTFTIIVHLKHIKQ
                     TVRVSSD"
     gene            complement(10977..12062)
                     /locus_tag="PXA33_000889"
     CDS             complement(10977..12062)
                     /locus_tag="PXA33_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDL5120235.1"
                     /translation="MEYRYYITTALVIAVLQLFLFNFAKTVSWLFNLSSKNRKTVGIL
                     TFLVANGLVILHLTRTFALFRIMAFILVLLLFAFFISFFTACIYKIFNGNTTVNRWLK
                     IAYPFAFFGLLGLGYYNAYTPTVVHYSVKLDKPLEKPLRIGMASDLHLGKLFGTKQLD
                     QLAEIFNQQKVDLILLPGDIMDDNTEVYVADKMQPHLAKLKAPLGVYATLGNHDFFGA
                     QETIAKEITDAGITVLWDQTAEINGKFTLIGRNDDLVKNRPSAEQLLQNVNTKLPVIL
                     LDHRPTEIEQHAKLPIDIQVSGHAHKGQVFPASIITKLIYRLHYGYEKIGFGHYFVTS
                     GYGFWGVPMRLGSQSEVMIIDVEGENP"
     gene            complement(12084..13064)
                     /locus_tag="PXA33_000890"
     CDS             complement(12084..13064)
                     /locus_tag="PXA33_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HDL5120236.1"
                     /translation="MLFDSIQFSIGVTLPTILLMLFGIFPRRRKFIDDTFCNMASKVV
                     FNFTLPPMIFLNMLKSPLDFSSQINLISTGVGGSILIYLWAEWWAARHIENRRYRCIF
                     TQGTFRTNAAILGIALILNAYGDKALAAASVYISCLVITFNVLAVITLTSSLSEKKVN
                     FGRLILSILRNPLILAVIAGLTFNYFELSHQIPTPLESAGKLMASLTLPLALICTGAS
                     INFKQLKQFNQGVESTINKIVLFSASIRLIFAPIFLLLLGKFVFQLPPMELGIVFVAA
                     SAPVASATYAMTRNYGGDGEAAANLIGITTLGSMFSASIGLFLLRQIGWV"
     gene            complement(13167..14265)
                     /gene="prfB"
                     /locus_tag="PXA33_000891"
     CDS             complement(join(13167..14189,14191..14265))
                     /gene="prfB"
                     /locus_tag="PXA33_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014550889.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HDL5120237.1"
                     /translation="MFELNPIKNQLTDLSERTAVIRGYLDFEHKVERLEEVNAELEQP
                     DVWNKPEKAQALGKERAMLEQIVNTIKALEQGIEDVEGLIELAVEAEDQDTFNEAQVE
                     ADELEKKLADLEFKRMFSGPHDSADCYLDLQAGSGGTEAQDWTEMLLRMYLRWAESKG
                     FKAELMEASDGDVAGIKSATVKISGDYAYGWFRTETGIHRLVRKSPFDANNGRHTSFS
                     AAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNKTESAVRITHIPSGIVVQCQN
                     GRSQHQNKDQAMKQLKAKLYELEMSKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DHRTGVEHRTTQQVLDGDLDKFVEASLKAGL"
     gene            14521..15429
                     /locus_tag="PXA33_000892"
     CDS             14521..15429
                     /locus_tag="PXA33_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HDL5120238.1"
                     /translation="MAKRPSDIDATLLQLEILRRIPKLPAKIDAKSLHQQLLDAGFER
                     DIRTVQRSLKMLCEHFDIECDERSKPFGYSWKIRSEGLALPILNEQQSLLLKLAEQQL
                     KYLLPANLMASMKPFFEQADRMVSGAKDKPEYQWLNKVCSTPTSLPLIPAKIKEEIFN
                     SVSQALFQNKLLKIEYQNQHGKKHQATIMPLAISQQGASIYLVCRYEGFDDNRLLALH
                     RIRKAEISTFSFERPKDFNLQAYQDAGHLGFGNGEKASLTFSISRDAGFHLTETPLAK
                     DQKILEESETHYRIQAIVADNDMLTW"
     gene            15730..16371
                     /locus_tag="PXA33_000893"
     CDS             15730..16371
                     /locus_tag="PXA33_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HDL5120239.1"
                     /translation="MPFQLTFCQQAGNQHQKNQDALFNGKAVYQWQLKNAESYLLDND
                     KVIFGVADGVSSHPRNHLASHYLMALLSQCQSLDSQWLRQAHGDFCEKLAKDYFGASC
                     TFVACELHHNGRGRMVNVGDSRAYKITANGEWQQLSFDHTILAEMKQKGLVKEGSNYA
                     SMYNGLTDCIVADFDADDFRIHIAEFQLEKGESILLCSDGLHEQLAPEAIQAI"
     gene            16538..>16743
                     /locus_tag="PXA33_000894"
     CDS             16538..>16743
                     /locus_tag="PXA33_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacetylase"
                     /protein_id="HDL5120240.1"
                     /translation="MKNDFHYAAELIRNADGILITAGAGMSVDSGLPDFRSVGGLWNS
                     YPPLKKFNLQFMQIATPLAYQERP"
BASE COUNT         4774 a         3518 c         3566 g         4885 t
ORIGIN      
        1 gttggaaata tgcttttaaa ttaatcacgc cgatttttat gggctatttt gcggcagggg
       61 ttgcttacgg aatgcttgcc accaatgccg gaatgcctgc gtggtttacg attgtaatga
      121 gttttaccgt ggtatcgggg acggcacaat atgcagcgat tccatttttt gtctccggtg
      181 ttggggcggt gtcggtcttt ctcagtacgc ttttaatgag tctacgtttt agtttttaca
      241 ccttaaatat gatggagcat aagccgatat ccaaagcgaa aagtctggtc gctattgcag
      301 gtttaactga tgaaggcttt gcggtgttat cttcattacc gaaagagcaa aagcaagcat
      361 tattctttaa aatcgctgtg ctatgtgtgg cttattggac attttccacg attgtcggtg
      421 tgttgttagg cgattcagtg gcgaactata ttccgcattt ggattttgct ctgccttgtt
      481 tgtttgcaat tttagcctat gagcaataca aaaaccaaaa acaatggaaa ccgattgtga
      541 ttgcgttaat cggattttta cttgctcgtc agattaccga aaccagcgtg ttattggtgg
      601 caattttagt agcgattgtc attgtggctt tattacctca atcttcttca atcaaactag
      661 agaaaggagt agaaaatgag cacaaatgat actttgataa tgtttgcagc attagtgctc
      721 ggctcgcaaa tttgtcgttt tttgccggaa tttttaccga aaaaggtgtt atcttcgccc
      781 attttgcaaa aattaaacaa aatgttaccg cttgtgatta tgcttttatt gcttttaacg
      841 agcctaaact ttcctaaaaa cggggaaggc tttaacttaa tattggcaca aattctggct
      901 ttaataggcg tattaggtag ctatgtttgg cttaaaaata cctttttaag cgtagcgttg
      961 gggattttcg gtgtgaatgt atttttatcc ctgttaggat aaataccaaa ggtcatttta
     1021 taggagcgaa acaccttcgc tcaatcatta acagatgtgg ggcgaaaaat ttttcgcccc
     1081 ttatttatca caagcggtct ttttctttca attttttgca aattaaatcg ctaaatcaaa
     1141 cgcatagaag gcaactaaaa taatcgcaat aattactgta cctaggttta atttattggc
     1201 ttcgcctgaa attaagcgac caatcacaag ggtggcgaaa cctagcataa tgcctgtcac
     1261 aatgttggcg gttaatacga tgaacaccgc acagactaaa cctgacattg cacctacaaa
     1321 atcgctgaaa tctaatttag agacattact tagcattaat aagcccacat acattaatgc
     1381 cggtgcggtg gcatagcttg gtactaagaa tgcaagaggt tggaagaaca gcataaataa
     1441 gaaaccgata ccaaccacta cagcggttaa acctgtttta ccgccaacgg ctgtacctgc
     1501 tgccgattca atatacactg ccgctggtgc tgtaccaaat aagcccgata aaattgatcc
     1561 gaatgagtca gaagttaggg ctttatcgcc attgataatt tgtccgtctt tatctaataa
     1621 accggcttgc cctgcaaccg cacggatagt acctgttgca tcaaaaatag cggtcattac
     1681 taacgcaaac actaccggta aaatggcggt atttaatgct cccataatat caagatttag
     1741 gaattgcgaa ttttcaccaa aactcggcat tttgaaaaag cctgtgtatt ttacgttagg
     1801 gtcaaaaatt aaacctacga tggtaatagc aataatgacc cataaaatac tgccttttac
     1861 tttcagcttt tctaacccga taatggcggc tagaccgatt aatgacacta aaaccggaaa
     1921 tgaagtgaag tcccctaatt taaccggaat atccataccg ctattgacca ctaatttaac
     1981 gttagtggcg gcaataatca gtaagaataa accaatacca attccagcac cgtgggcaat
     2041 gctgcttggc aaattacgta aaatccaagt acgaataccg gtagcagaaa tcaaggtaaa
     2101 tactacgccc attaagaaaa tcgccccgag tgcaacagga atagaaactt gttgccctaa
     2161 tactaagcta aaggcagtaa aagcagtaag tgaaatcgca caaccgattg ccattggggc
     2221 atttgcccat aaaccgatta agatagagcc gagacctgaa actaaacagg tggcaataaa
     2281 taccgattcg gcaggaaagc ctgcttggct tagcattccc ggcacaacga tgacggaata
     2341 aaccatggct aaaaaggtag ttaaaccggc gatgatttct tgacgagcgt ttgaaccacg
     2401 ctcctcaaat ttaaataacg acataagaac ctcaaaagaa gaaaatgaaa ggggaaattt
     2461 taggatgcgg aattttacag aaatttaaaa taattagcaa acgtttgcgt aaaaagtgaa
     2521 agaaaagcag gaataaatcc tgcttttgtt tgatggttag taatcgatga aagcgtcttg
     2581 atctgcccct tccttttcaa ctttaggctg aaccatatgt tcacgtttta gcccaagttg
     2641 tagtgccaca ccaattgcaa tgtagatgga tgaataagta ccgaaaccga taccgatcaa
     2701 taatgcaagt gagaaactat gaagcgttgg accacctaga tagaataagg ctagtaccac
     2761 aaagagggtg gttactgatg tcattaaagt acgcgaaagt gtttggctca aggaaatatc
     2821 gataatttca attgagctgg agcgacgaac tttagaaaag ttttcacgta cacggtcaaa
     2881 caccacaatt gagtcattta acgagtaacc gacaacagat aaaatcgctg ctacaaaggt
     2941 caagtcaatt tcaatctgta agaaagagaa gattccgatc gtcactaata catcgtgaaa
     3001 taaagccaca ataccgccta ccgctaaacg ccactcaaaa cgtgttccca cgtaaataag
     3061 tagcatagca agtgttgcca aagtggcata aatagcccct tgggttagct cttcccccac
     3121 gtttgggcca acgaattcaa tgctcttgat agtcgcattc ggatctaatt tttgatggat
     3181 catctccatc actttattcc caacagacat atcaccggca ctggcggcaa gtcggatcat
     3241 taaatctttt tgactgcctg tggtttgcac taaggcattg ccgtagccgc tattttcaag
     3301 ctccgtacgc agttttgcta aatcagcagg ttgtgaaaat tgagtttcga ttactgtccc
     3361 gccggtaaaa tctaaccccc agttaaagcc ttttgtcgca atagtaaaaa tgcataaagc
     3421 agttacaatc agcgaaaaaa tgaatccgac atagcggtat ttcataaagg ggattaattt
     3481 ataaggcagt ttaatttctg ctacatcttg ttgtacattt tcaatagcca tttttccacc
     3541 ttaaatccaa agtttcttca cgcgtttgcc accgtaaacc cagtttacaa gcattcgagt
     3601 acctgtaatt gcggtaaaca tagaaataat tacccctaat gcaagcgtta ccgcaaagcc
     3661 ttttaccgga cctgtaccta caccataaag cacaagagaa gtcaaaatag tggtgaggtt
     3721 ggagtcaaaa atacttgaga atgcgccatt ataaccttcg tggattgctt gttgaattgg
     3781 gcgaccgtta cgaatctctt ctttgatccg ctcgtagatt agtacgttgg catcaactga
     3841 catacccaca gcaagtacga taccggcaat tcccggcata gtaagggttg caccgattaa
     3901 tgacattaaa ccaatcgtta agatcatatt cacaatcaag gcaagagagg caaatatacc
     3961 aaatacttta taaaaggcta gacagaacag gatcgttaag cctaagccta aaatactagc
     4021 ttccatacct tgtttaacat tatctgcccc aagagaagca ccgatagtac gctcttccac
     4081 aatcacgatt ggagcaatca acgcacctga acgtaataat accgcaaggt tttgagcttc
     4141 cgctggtgaa ttaatccctg taatctggaa ttgattacct aaacgggttt gaatggttgc
     4201 aacgttaatt acctcttggt gtttttctaa aatgacttta ccgttagcat ctttacgacc
     4261 tgaatcttta aattcggtat aaagtgttgc catcggtttt tggattgcca ttttagtaat
     4321 ttcacccatc atctcgccgc cttcggtatc gagtcggata ctcacttgcg gcaagccacg
     4381 ctcatcagtg cctgaggttg catcaataat atgttcaccc cctaacgaag gtttacggcg
     4441 taatacaact ggctcgccat cacgggtgtt ttgcagtcct gaatcagccg gcacaatgcc
     4501 tttcgccgca gaggccacat tggcttcggt attgactaac cggaactcaa gggttgcagt
     4561 tgcccctaaa agctctttgg cacgagcggt atcttgcaca cccggtaatt caactacgat
     4621 acgatctgca ccttgacgct gaatggtcgg ctcagaaacg cccaattcct caacacgttt
     4681 acgcaaaata gaaaggtttt gttcgattgc catcgtgcga gattcagaaa gagcttgtgc
     4741 tgatggcgtt aaggtgactt tattatcatt taccaaaata tcaagcgttg ggtgagagag
     4801 acgcactaaa cgcactgctt tttcggcggt atcgttatcg gcaaattcaa cctcagttgc
     4861 aaaattttcg ccttttttga ccgcttgata ttgcaatttt tctttacgga aatcactacg
     4921 caggctgtct tgcaaagtgt cttgttgttt tgcaagcgcc gtgttcatat ccacttccat
     4981 taagaaacga acaccgccaa gcaagtccaa gcctcgcttc atcgggctac cgccaatgtc
     5041 agacaaccac tgtggcgttg ctggtgcaag gtttaaggca acagagtagt tattccccaa
     5101 tgcttctgag attttttctt tggcaggcaa ttgctgttcg ctgctttcga gacgaaccaa
     5161 aatcgagcca ttttctaaca cggcagattt cggctgaata ttcattgctg aaagtgtgga
     5221 ttgcacttga gacagtgtct caggtgtggc ttgttgccca cgagtaccgg aaatttgtac
     5281 cgaaggatca tcgccatata aatttggaag ggcatataaa gcaccaatgg cgatcacgaa
     5341 gatcaccatt aaattcttcc atagagggaa acggtttaac acaataactc cccgaatgtt
     5401 aaagttggat aagagggttg attaaagtga ttttaatgaa ccttttggta acaccgcaac
     5461 aacgaaatca cgtttgatgg tcacttcagt tgtgtcattt aatgcaatca cgatgttatc
     5521 gttatcagcg gtcactttgg taattttacc gattaaacca ccgctggtta atacttcatc
     5581 gccttttgtc aagcttgcca gtaaatcacg ttgttgtttt tggcgttttg cctgaggacg
     5641 ataaatcata aaatagaaaa ttgcaccgaa aataatcagg ataaagatca tttccattcc
     5701 gccaccttgt tgcataaaaa ctccttaaat gtttgagtgg tataaaaata agtgttataa
     5761 aaaacgcctg ttaaaatacc ataaattcaa tgaattaacg agtagaaaaa ttcatcgaat
     5821 gcttttcttt taaacaaaaa tttgagtaga atcagcactt ttagtttagg attaggttct
     5881 tggcgtgttc ttgcgctgac acaataggag aaaaaatgaa aaaattattc gctcaagcgg
     5941 ttatttttgg ggcttttttt gcaagttccg cttttgctca caatgtgcaa ttaaatcaag
     6001 cattacctgc tgtttcggtt gcaaaagacg gggaattggc agtggcaggt ggaaaggtta
     6061 gctataaaaa ttggcattca tcatctctgg caggtaaagt ccgtgttatt catcattttg
     6121 ccggtagaag ttcggtgaaa gaaaaaaatg aagatttgat gacagcaatt aaaaatgccg
     6181 gttttgatcg ctcgaaatac caaacggtga cgattgtaaa tgctgatgat gcgattgtcg
     6241 gcacaggggt atttgtgaaa aatagtgtgg aaaacggcaa acttgacaat cctcacagcc
     6301 aagtagtgtt agaccagcaa ggcagtgtga aaaaagcgtg gggcttaaaa gagaaagaga
     6361 gctttattgc agtgctggat aaatcgggta aggtacaatt tacctctgag ggcaaactct
     6421 ctgcaaaaca gattcaagaa gtgattgggt tggtgaaaac attaacgcaa taaccagtgt
     6481 ttttatgggc ggattttgta tccgcccatc attctagata ataaatcgat aacttatcgt
     6541 gtgatgatac tcttctcctg cttttgttat cccgccaaat tggagtaatt caggctggtt
     6601 tagtgaattt gggaaaaact caggctctaa tgctacacca gcgtaatctt catattcgcc
     6661 tttagtcaaa ttaggttgtc cttttagcca gttgccggtg taaagttgta atgctggcat
     6721 ggaggtgcgg agttccattt ttaagtcttc cacggctaaa caagcggtcg gattttcact
     6781 attttttgca agatgaaagg agtgatcgta accttttacc gctttttggt cgtcatcagc
     6841 caataaatct tgcccaattt ttttagcatt gcggaagtca aaactggtac tctccacttt
     6901 tttcagtgga gcattaggaa taccgctggc atcaaccggt aagtatccgt ctgcattaat
     6961 tatcagctcg tgatctaaaa tcgtctctga accttgcaaa ttaaaatagg cgtgattagt
     7021 taagcaaagc ggtgtatctt ggttagcaac ggcatcaaag ctgatttgaa gctcattctc
     7081 tgttaagcga tactccacaa aagctttcac ttcgccgcca aatccttcct cgccatctgc
     7141 aaaaaaataa ctaaatttga ccgcttgttc gctttggctt tcaagttgcc acactatttt
     7201 atctgcccca agctcgccac cgtggagatt atgttcaccg ttatttttag cgagctggta
     7261 ggttttgccg tttaagcgat attcggcatt ggcaatgcgg ttagcgtaac gcccaatggt
     7321 cgctccaaag aaagcggtct gtttttgcca attttttgca gacgtggtga ctaatacttc
     7381 ccgttcctcg ttcggcattt tgacaatgca cgaaagccaa gaagccccaa catcagaaag
     7441 ggtaatgcgt aaaaacgcat tttcaagggt gaataatttc atcggttgac cttaattacc
     7501 tagcacggca tagccgtact aggttaagca taattcagcc ctgctagggc tgaggttatt
     7561 tattttagag tagcggagct acttccttat gcttaacccg gtacgcttta gcgtgccggg
     7621 cattctcgca ccccatcgga ggcagtacaa acgtggaagg tttctttaat gccggtttgc
     7681 ttttcgtaat tgttagcaac ggcttggcgg agatcttcca ctttgtcatt tggcactagg
     7741 cacacgacac aaccgccaaa gccgccgcca gtcattctgg ctccgccgtt tttaccgata
     7801 gcttgttggg cgatttcggc taagcaatcc acttcagggc aggtgatttc aaaatcatca
     7861 cgcattgagt cgtgagattg agccatcaac tccccgagtt tgaccatatc attcgctttt
     7921 aaggcctcta ctgcttccag aactcgttga ttttcgctga tgatatgttt tgctcgttta
     7981 tacgccagct cattctcttc actaagttct gaggctcgtt caataaattg ttccgctgtg
     8041 acatcacgca attttgcttt gccgaagaag tgggcggcaa attcgcactg ttcacggcga
     8101 ctgttgtatt cgccggtaac caagtcgtgt tttacgttgg agttaataat cgcaatcgaa
     8161 tagccttgtg gcacaggggt tggggtgatt tctaaactac ggcagtcgat catcactaaa
     8221 tgatcttgtt gcccgagagc ggaggtgagc tgatccatat tgccgcagtt gcagccgaca
     8281 aatttattct cagcctcttg cccgattaat gcgatttgcg gtaaagaaag cggtaaatcg
     8341 ccgagtaatt gagcggtttt accaatcgac acttccaatg cggcggaaga acttaaacct
     8401 gatgacatcg gcacatcgct ggtaatggct aaatctgctc cttgtttgaa ttcggggtgt
     8461 ttggcttgaa taaatttcac cacgccacgc acataattcg cccatttttg ctcgcttggc
     8521 tcaatcgcct ggcttaaatc aaattcatct tgttggttga gatcaatggc gaacacccgc
     8581 catttgttgt cactacgttt ggcaaagcta accgctgtgc cgtaattgat ggcacaaggc
     8641 attacaaacc catcgttgta atcagtatgt tcgccgataa tattcactct gcccggggca
     8701 aataccgtgc gttctgcttt ataaccaaaa tgttgttcaa attgttgcaa agcgatttct
     8761 tgtggtttca tttcaattcc ttatttatct ttatagtgtt tggtatcact tactgcattt
     8821 aaacgttcgg cagcttgttc cggggttaaa tcacgttggc tttctgccat catttcataa
     8881 ccgaccataa atttacgcac tgttgccgaa cgcaagagcg gcggatagaa gtgagcgtgt
     8941 aattgccagt gagcattttc cttgccgtta aacggagcga agtgaaagcc cattgaataa
     9001 ggaaagctga tgttaaacag attgtcgtaa cgggtggtga gctttttcaa tgcaagggct
     9061 aaatccgctt tttccgcttc gtttaaatcg gtaatggatt tgaactgctt ggctttcggc
     9121 aacagcaagg tttcaaacgg ccaagccgcc cagtaaggca caaccgcaat ccaatcttcg
     9181 gtttctgcca caattcgctc ttgtttgtct aattcacgtt gtgcgtagtc aagcaacagt
     9241 ggcgagccat attgagcaaa atatttcgct tggcattcgt cttcaatcgc aatttcatta
     9301 ggtagaaaat tacttgccca aatttgcccg tgtgggtgag ggttagaaca ccccatcatt
     9361 gagcctttgt tctcaaaaat ttgcacccac tgataatgct ctttcagctc attcgcttgc
     9421 tcttgccaaa ctgttaccac atcagtaatt tcctcgatgg aaagttgcgg taaggtttta
     9481 ctatgatcag gcgaaaaaca gatcacacgg ctttcgcctt gtgtatgaga aatctgaaac
     9541 agcggatcat cattcggtat tggtgccggt gtgtctggta gcagggcaga gaagtcgttt
     9601 ttaaacacaa acggcttggt gtaattcggg ttttgttcgc cggtaatccg cttattgccg
     9661 gggcagaggt aacaacttgg gtcgtagctc ggtttttcat cttgcacgat ttcttcttgt
     9721 tgcccttgcc acgggcgttt tgcccgatgc ggagagacta aaatccattg attttttaat
     9781 ggattaaaac gacgatgtgg gtgatcgttg aggacaaatt cgctcatata aaactcctcg
     9841 aaattagtta tgtaaagagt atagcgaaag tcagacaaaa tgactgtgac atagctcaca
     9901 aaataatgat gtcaagcggt caaatttcag aaaaattttg caagttttcc tccattttgc
     9961 gatttaaggt agcttacgtg gaattgaatg tggcttttgg tagaatagat gaatatgcaa
    10021 gatattaaga caaaaataga tggaaagact gaataaattt gatgcttggt taatgaatca
    10081 taaacctaat gctaggttaa gcggagcgag tcgtttgttg acagagtttt ggttctttgg
    10141 gttaaaagaa gctagagcct gtttatttgc gggattattt tttattgcga tgttttcggt
    10201 accaaaaacg ggattatggg gcttacctcg ttatgatatt ttgctgattt ttgccatagt
    10261 ggtgcaaatt gcaatggtga aactggggct ggaaacgtgg gacgagctaa aagcaataag
    10321 cctatttcat ttggttggtt ttgtgttaga agtctttaaa acatcgagta atattcaatc
    10381 ttgggcatat ccggattttg cctatactaa attgttcaat gtgccattat ttactggttt
    10441 tatgtatgca gcagttggtt cttacattat ccaagcgtgg cggttgtttg atttgaaaat
    10501 taaaagccac ctgccctatt ggttagccac tttaacggca ctgatgattt acctgaattt
    10561 ctttacgcat cattacattg gggattaccg ttggtatatt gccgcttttg cattagggct
    10621 gtatgcacgt acttttgtat ttttcacccc ttatgacatt acacgaaaaa tgccactgct
    10681 gctttctttc ttcctgatag gttttttcat ttggttggcg gaaaatatcg gtacgtttgc
    10741 cggaatttgg cgctacccga atcagtttgg ggcttggtct atggtgcata tgagcaagtg
    10801 gagtgcttgg gcgttattgg tgatgatgac ttttaccatc attgtgcatt taaagcatat
    10861 caagcaaaca gtcagagtct cgagcgatta acaagctgtg ggttttggcg aaaattttac
    10921 tcatcagtta ttttcagtgg aattaggggc aaataataat ttgcccctat atcaaattat
    10981 ggattttccc cttccacatc aataatcatc acttccgact gtgagcctaa ccgcatcggc
    11041 acgccccaga atccgtagcc ggaggtgaca aaataatgcc cgaagccgat tttttcatag
    11101 ccgtaatgta atcgatagat cagtttggta ataatacttg cagggaacac ttgccctttg
    11161 tgagcgtgtc ctgaaacttg aatatcaatc ggcaatttag catgttgctc aatttcagtc
    11221 gggcggtggt caagcaaaat taccggtagt ttagtgttca cgttttgtaa aagctgttcg
    11281 gcagaaggtc gatttttgac taaatcatca ttacgcccaa taagggtaaa ttttccgtta
    11341 atttcggccg tttgatccca taaaacagta ataccggcgt cggtaatttc ttttgcaatc
    11401 gtttcttgag ccccaaaaaa gtcgtgattg ccaagggtag catacacgcc aagaggagct
    11461 tttaatttgg ctaaatgagg ctgcatttta tcggcaacat aaacttcggt attgtcgtcc
    11521 ataatatcgc ccggcaataa aatcagatcg actttttgct ggttgaaaat ctctgccagt
    11581 tggtcaagtt gcttggttcc gaacagtttt cctaaatgca gatcgcttgc cataccaatg
    11641 cgaagcggtt tctctaatgg tttatccaat ttcaccgaat aatgtactac tgttggggta
    11701 taagcgttgt aatagccaag cccaagtaag ccaaagaatg caaatgggta ggcgattttc
    11761 agccagcgat tgacggtagt attgccgttg aatattttgt aaatacaagc ggtgaaaaaa
    11821 ctgataaaaa atgcaaaaag taacaatacc aatatgaatg ccattattct aaatagcgca
    11881 aaagtacgag taagatgcaa aatcactaat ccgtttgcga ctaagaaagt gagaatacca
    11941 actgttttac ggtttttact tgataaatta aataaccagc tgacagtctt tgcaaagttg
    12001 aataaaaaga gttgtaacac cgcaatcaca agggcggtgg taatgtagta acgatattcc
    12061 atatttttcc ttcaataaat tggctaaacc cagccgattt gtcttaataa aaataagccg
    12121 atgctggcac taaacattga gccgagagtc gtaatgccga tcagatttgc ggctgcctcg
    12181 ccgtctccgc catagttgcg tgtcattgcg taggtggctg aagcaacggg agcagaagca
    12241 gccacaaaga caatgcctag ttccatcggc ggaagttgaa acacaaattt cccgagtagt
    12301 agcaagaaaa ttggagcaaa aatcagccga atcgaggcag aaaacagcac aattttattg
    12361 atggtgcttt ccactccttg attaaattgt ttcaactgtt tgaaattaat gcttgctccg
    12421 gtgcagataa gtgctaatgg tagtgtcagg ctcgccatta atttaccagc agactcaagc
    12481 ggggttggaa tttggtggct taattcaaaa tagttaaaag ttaagcctgc aatgacggct
    12541 agaatcagcg gatttctcaa tatcgacaat attaatctgc caaaattcac ttttttctcg
    12601 cttaacgatg aagtgagggt aatcactgca agcacattaa aggtaatcac caagcacgaa
    12661 atataaactg aagcggcagc gagagcttta tcgccataag cattgagaat taaggcaatg
    12721 cctaaaatcg ccgcattggt acggaaagtg ccttgagtga aaatacaacg ataacggcgg
    12781 ttttcaatat gtcgagccgc ccaccattcc gcccataaat agatcaaaat cgaaccgccg
    12841 actccggtcg agatcaggtt gatttggctg gagaaatcta acgggctttt tagcatattc
    12901 aagaaaatca tcggtggcag ggtaaaatta aataccacct ttgatgccat attgcaaaag
    12961 gtatcgtcaa taaattttcg tctgcgtgga aaaataccga acagcattaa caaaatggtt
    13021 ggtaaggtaa cgccaatgct aaattggata gagtcgaaaa gcataatgtt tgccttaaat
    13081 gatggcacga gtgtcttttg cttgtaccgt agattatcaa taaagtacca gcgtggacgc
    13141 tggtactatc atcaatggaa atgaaattac aatcctgcct ttaagcttgc ttcgacaaat
    13201 ttatccaaat caccgtccag cacttgttgg gtggtgcgat gttccacacc ggtacggtgg
    13261 tctttaatcc gtgaatcgtc tagcacataa gagcggatct ggctgcccca gccgatgtcg
    13321 gatttgttgt cttccatcgc ctgtttttcc gcatttttct tgctcatttc cagctcatat
    13381 agcttcgctt taagctgttt catcgcctga tctttatttt gatgttgtga acgaccgttc
    13441 tggcactgca ccacaatgcc gctcggtata tgggtaattc gcaccgccga ttcggtttta
    13501 ttgacgtgct gaccacccgc ccctgaggca cgatacacat caatccgcaa atccgcaggg
    13561 ttgatttcaa tatcaatatc gtcatcgact tccggataaa cgaaagccgc cgagaacgag
    13621 gtatgacgac cgttattggc atcaaacggg cttttacgca ccaagcggtg aatacccgtt
    13681 tcggttctga accagccgta agcatagtcg ccggaaattt tcacggtggc ggatttaatc
    13741 cctgccacat caccgtctga ggcttccatt agttcggctt taaacccttt gctctctgcc
    13801 caacgcaaat acatacgcag taacatttct gtccagtcct gagcttccgt tccacccgaa
    13861 ccggcttgca aatccaaata gcaatcggca ctgtcgtgtg ggccgctgaa catccgctta
    13921 aattctaaat cggcaagttt tttctctaat tcgtctgctt ccacttgggc ttcattgaag
    13981 gtatcctgat cttctgcttc caccgccagc tcaatcaacc cttcaacatc ttcaatccct
    14041 tgctctaagg ctttaatcgt attgacgatc tgctccaaca ttgcccgctc tttaccaagc
    14101 gcttgtgctt tttccggctt gttccataca tcgggttgtt ccagctcggc attgacctct
    14161 tctaagcgtt cgactttatg ctcaaagtca aagatacccc ctgatcaccg cggtgcgctc
    14221 gcttaaatca gttagttggt ttttaattgg gtttaattca aacattgtcg ttcctgtttt
    14281 tgcttatttt aaaaagatga attatagctg aagttaagca agcggtcgaa ttttgcaaga
    14341 aatggagcaa tgatcgtttc tagtaatgag atttggttca taaaagttgc aactgtttct
    14401 gctatttggc tttgatagct aaaaatcttt aaaaaaacaa gcggtcgttt taccccattt
    14461 tttgcaaaac gaccgtagct tcttgctata atcgctgaaa aaatgctgca aggagatttt
    14521 atggcaaagc gcccaagtga tattgatgca accctattgc aactggaaat cctacgccgt
    14581 atccccaaat tacccgctaa aattgatgct aaaagtttgc accaacagct attagatgcc
    14641 ggctttgaac gggatattcg cactgttcag cgttcgctca aaatgttatg cgagcatttt
    14701 gatattgaat gcgatgagcg gagcaaaccc tttggttata gctggaaaat ccgctcagag
    14761 gggctggcat tgccgattct caacgaacaa caatcattac tgctaaaact tgccgagcaa
    14821 cagctgaaat atctgttacc cgcaaattta atggcatcaa tgaaaccctt ttttgagcag
    14881 gcagacagaa tggtttccgg ggcgaaagac aagcccgaat accaatggct aaataaagtt
    14941 tgctccacgc caaccagcct accgctgatc cctgcaaaaa ttaaagaaga gatttttaat
    15001 tccgtaagcc aagccttatt tcaaaataag ttgttaaaga tcgaatatca gaaccagcac
    15061 ggcaaaaaac accaagcgac aattatgccg ttggcgattt cacaacaagg tgcgagcatt
    15121 tatttggtct gccgttacga agggtttgac gacaaccgcc tgttggctct tcaccgcatt
    15181 cgtaaagcgg agatttctac ttttagtttt gaacgcccga aagattttaa tttgcaagcc
    15241 taccaagatg ccggacacct cggttttggc aatggcgaaa aagctagcct taccttttcg
    15301 attagcagag acgccggttt ccatttaacc gaaacaccgc ttgccaaaga tcaaaaaatt
    15361 ctcgaggaga gtgaaactca ctaccgtatt caagccatag tcgctgataa tgatatgcta
    15421 acctggtagc tacgcaaatt tggcgaagat atttgggata tcgagaaaga agcgctaaaa
    15481 taagctaaac aagcggtcgg ttttgtggga atttttgcta aatagtatcg accgctcctc
    15541 cctaaacaga cctttatcat aaatacaact ctttcaatca gatattctta ctgtctattg
    15601 atgaacgatt cgaatttttc tagtggttga aatttaaact aattttcttg ctttttggca
    15661 tttttcagac ttttaattct ctccattagc ctgttttttt acgctctttt tcataaacag
    15721 gaataaccaa tgccttttca acttactttc tgccaacaag ccggcaatca gcaccagaaa
    15781 aatcaagatg cactgtttaa cggcaaagcg gtgtaccaat ggcaacttaa aaatgccgag
    15841 agttatctgc tggataacga caaagtaatt tttggggttg cggacggcgt ttccagccac
    15901 cctcgaaatc atcttgcctc acattattta atggcgttac tcagccaatg ccaatccctt
    15961 gatagccaat ggttgcgaca ggctcacggc gatttttgcg aaaaattagc gaaagattat
    16021 tttggtgctt cttgcacttt tgttgcttgt gaattacacc ataacggcag agggcgtatg
    16081 gttaatgtgg gcgacagtcg agcctataaa attacggcaa acggggagtg gcaacaactg
    16141 agttttgatc acaccatact ggctgaaatg aaacagaaag gcttggttaa ggaagggagc
    16201 aactatgcct caatgtataa cggcttaacc gattgtattg ttgccgattt tgacgccgat
    16261 gatttccgca ttcatatagc cgaatttcaa ctggaaaaag gcgagagcat tttgctctgt
    16321 tctgacggtt tgcacgaaca actcgcccca gaagctattc aggcgattta ggacggatac
    16381 gaaagcccgt ctcatcgtct gcaaatttgc agaaaaatgg taaaaatgca ccgcttgtat
    16441 gacgattttt ctgcggtgat ttgtgaaatt gtgtaattaa aacgacatta tttgtcgcat
    16501 tggtgcttaa aatccgcata aaacagagga gaaaaaaatg aaaaacgatt tccattatgc
    16561 cgctgaatta atccgcaatg cagacggcat tttaattacc gcaggggctg gaatgtcggt
    16621 ggacagtggc ttgcccgatt tccgtagcgt gggagggtta tggaattcct acccaccgct
    16681 caaaaaattt aatttgcaat ttatgcagat tgccacaccg cttgcctatc aggaacgccc
    16741 tga
//
LOCUS       DANLRK010000038        16739 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.038, whole genome shotgun sequence.
ACCESSION   DANLRK010000038 DANLRK010000000
VERSION     DANLRK010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 16739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16739
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.038"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(196..378)
                     /locus_tag="PXA33_000895"
     CDS             complement(196..378)
                     /locus_tag="PXA33_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5120241.1"
                     /translation="MYQFGYLRTKIGTDTQRAMLWNCPTYVLIIPFLLNTSVKKQYHA
                     FWQKYHQHNNDVSFPY"
     gene            528..1568
                     /gene="selD"
                     /locus_tag="PXA33_000896"
     CDS             528..1568
                     /gene="selD"
                     /locus_tag="PXA33_000896"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020909986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:573..575,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HDL5120242.1"
                     /translation="MSEPIKLTQFSHGAGUGCKISPKVLGTILQTQLEQFVDPNLLVG
                     NETADDAAVYDLGNGQAIISTTDFFMPIVDAPFDFGRIAAANAISDIYAMGGKPIMAI
                     AILGFPVNKLPAEVAQQIVEGGRFACQQAGIALAGGHSIDSPDPIFGLAVTGIVPTER
                     VKRNASAVAGCELFLTKPLGIGILTTAEKKGLLKPEHQHLASEVMCQINLIGSAFSEL
                     PEVTAMTDVTGFGLLGHLSEVCQGSGLRAEVDFEDIQVLDGTKEYIALGAVPGGTNRN
                     FDSYGHLISAMTDEQKAILCDPQTSGGLLVAVLPQAVEKVQQIANRANVPLFHVGRLL
                     EMEQGKPRIEVI"
     gene            1568..2041
                     /locus_tag="PXA33_000897"
     CDS             1568..2041
                     /locus_tag="PXA33_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDL5120243.1"
                     /translation="MFKPNVTMACVVHCKGKFLFVEEIEYGKRTLNQPAGHLEKDETL
                     LEGASRELFEETGIRAEMANLVKIYQWQAPRSQTDFLRFLFSVELEEWLEPNPQDADI
                     TQALWLTLAEFEDYIQQQGQCARSPLVVQSVKDYLSGERYPLSLLNAFEWARNGK"
     gene            2031..3239
                     /gene="ubiH"
                     /locus_tag="PXA33_000898"
                     /gene_synonym="visB"
     CDS             2031..3239
                     /gene="ubiH"
                     /locus_tag="PXA33_000898"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HDL5120244.1"
                     /translation="MENKTTTQFDVVIVGGAVTGSVLALALSSFTHHKISIAIVEKSL
                     PDYEQQGGFDARSIALAEGSLQKMGQIRPLAGSSLGEMISEIGTPIQQIQVADKGHFG
                     KTTLKASEQNVAQLGMVVELAKFGRKLTACLAQQANITLFCPNQVVGIERSQQHCGLS
                     LKNGEKLSCSLLVAADGIQSQIAKQCGVETVKVRDYAQSAIIANVELSESHQNQAFEY
                     FTPQGPFALLPLSGNMMSLVWCVKEPNELIQLSDSEFLARLQQQFGWKLGQFVRVSKR
                     FVYPLILQKAQSHIHHRLAVVGNAAQMLHPVAGQGFNLGLRDLTTLAELVALAFNQGK
                     DIGEFSLLSTFENERTQDQARMINATSGLISLFCCEMLPVQVVRNLGLLTVSHSKIAR
                     DFVANQALGW"
     gene            3502..4734
                     /locus_tag="PXA33_000899"
     CDS             3502..4734
                     /locus_tag="PXA33_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease"
                     /protein_id="HDL5120245.1"
                     /translation="MTTENMSTPKQAFIGLQMLFVAFGALVLVPLITGLDPNVALLTA
                     GVGTLIFQICTKRQVPVFLASSFAFIAPIQFGVQQWGIPVTMGALLCSGFIYMALSAL
                     VKAKGVNILHKYFPPIVIGPVIMVIGLGLAPVAVDMATGKSANIAYNTAIIVSMITLV
                     TTLIVAVFAKGLLKLVPILAGLTVGYILSVFMGLVDFTKVVEAPWFSLPKVSAPEFKL
                     DAILYLLPIALAPAVEHLGDVMAISQVAGKDFMKKPGLHRTLLGDGLATSAAAALGGP
                     PNTTYSEVTGAVMLTKNFNPKIMTWAACWAIAFSFCGKIGAFLATIPTFVMGGIMMLL
                     FGAVAVVGINTLIKAKVDLSIPRNLCIASVVMTFGIGGMLINIGEFSLKGISLCALVA
                     IVLNIVLPKEKVEDSAAH"
     gene            4801..5787
                     /locus_tag="PXA33_000900"
     CDS             4801..5787
                     /locus_tag="PXA33_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HDL5120246.1"
                     /translation="MQKLTFSPPLLNTPCPWCSELESLRELYACEYTGAVTTRTSMPQ
                     PYPHDWAKNQYVLFDSNAQKTASVNTQDATSLQTASLNTIGLSPNNLDTTISFVRTIS
                     NELTAPSSKPFIISVFGSPEEVGECYEKIVAFQTEVKMPLAMEINISCPNIPGEISPA
                     YSAEELSHYLHALQTSLKKLAQGDNTLPIGIKIPPFTYQNQYDELIKGLLQAVENGEN
                     LPISFITSTNTLGSSLVLNAGKAVLNSEVGTGVGGMAGTAIHALSLGNVYTIRQMLDQ
                     EPKLKHLQIIGVGGVNDKAGFERMKSVGADFVGLATALGVKGIGVFKEILSE"
     gene            complement(5828..6238)
                     /locus_tag="PXA33_000901"
     CDS             complement(5828..6238)
                     /locus_tag="PXA33_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005625316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2251 domain-containing protein"
                     /protein_id="HDL5120247.1"
                     /translation="MLYSVLTAHILLNEPYRNGAHSTQHEHLVVMFEIDEESGYFYAM
                     DLHQTEQPVVDSLFVFRVSDIEQNSLKEPRRLEICWSEDGYQAFLLINGYPHAVFDFR
                     QFVGYNHTKFPMPELGSMWVHKETTNELVEKWLG"
     gene            complement(6304..7614)
                     /gene="eno"
                     /locus_tag="PXA33_000902"
     CDS             complement(6304..7614)
                     /gene="eno"
                     /locus_tag="PXA33_000902"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HDL5120248.1"
                     /translation="MAKIVKVIGREIIDSRGNPTVEAEVHLEGGFVGLAAAPSGASTG
                     SREALELRDGDKGRFLGKGVLKAVSAVNNEIANALVGKDGSAQAEIDQIMIDLDGTEN
                     KSKFGANAILAVSLATAKAAAASKGLPLYAYIAELNGTPGVYSMPLPMMNIINGGEHA
                     DNNVDIQEFMIQPVGASTLKEALRIGAEVFHNLAKVLKAKGLNTAVGDEGGFAPNLAS
                     NADALACIKEAVEKAGYVLGKDVTLAMDCASSEFYDKERNVYDMKGEGKTFTSQEFTH
                     YLEGLCKEYPIVSIEDGQDESDWEGFAYQTKVLGDKVQLVGDDLFVTNTKILKNGIEK
                     GIANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQ
                     IKTGSMSRSDRVAKYNQLIRIEEALERAGTPAPFNGRKEIKGQA"
     gene            complement(7805..8983)
                     /gene="rlmC"
                     /locus_tag="PXA33_000903"
     CDS             complement(7805..8983)
                     /gene="rlmC"
                     /locus_tag="PXA33_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HDL5120249.1"
                     /translation="MSSLLHCSHFERGDCTSCQWIEKPYSTQLAAKEADLKRLILPFI
                     LQNKTEILPLVGSPISQFRNKAKMVVSGSVERPILGILKDQNDAQSGVDLTDCLLYPS
                     SFHRLFPILKDFIARAGLVPYNISKKKGELKYILITESQYNQSFMLRFVVRSEAKRAL
                     VERELPELLEKLPPNSVVSLNIQPQHAALLEGETEIFLTEQKVLEEHFNGIPLFIRPQ
                     GFFQTNPVVASQLYHTAQSWIEHLPIAKLWDLFCGVGGFGLHCANALQAKNSNVELTG
                     IEISASAIASATKSAEKLGLKQVKFASLDSAQFALNEAGSPPDLVIVNPPRRGIGKLL
                     AEFLNQLGSPYLIYSSCNAVSMANDFTFLTNYELHKVQLFDMFAHTAHYETLTFLVKK
                     "
     gene            complement(8976..10013)
                     /gene="nagZ"
                     /locus_tag="PXA33_000904"
     CDS             complement(8976..10013)
                     /gene="nagZ"
                     /locus_tag="PXA33_000904"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HDL5120250.1"
                     /translation="MLLIDIKDKELTQEEVEMLEHPLVSGLILFTRNFYDKAQIQALV
                     KSIRQRVKKPLLITVDQEGGRVQRFREGFTKLPAMQSFNQLATTPEQAMQLAKETGWL
                     MAAEMFALDIDLSFAPVLDLGHECKAIGDRSFGQEPAAILPVAEAFIDGMREIGMATT
                     GKHFPGHGHVLADSHLETPFDDRPKERIFNHDILPFKQLIAKGKLSAIMPAHVVYTQC
                     DSQPASGSSYWLKEVLRKQLNFNGVIFSDDLGMKGAGFMGNFVERSEKAIQAGCDLLL
                     LCNEPEGLVQVLDGLKYQPTQAQKERRLSLMKRKTVTWRELENSSRYQQTQAELTALQ
                     NNWLEWKEANV"
     gene            complement(10139..10870)
                     /locus_tag="PXA33_000905"
     CDS             complement(10139..10870)
                     /locus_tag="PXA33_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HDL5120251.1"
                     /translation="MFDWITNPEAWVALFTLTGLEIVLGIDNIIVISILVSRLPIEQR
                     QSARIIGLGLAMGTRILLLLSLAWMMRLVEPLFTIGNNPISGRDLILLIGGLFLIVKS
                     AMELKESISAESHEEKSESIKKASFFMVLIQIAIFDIIFSLDSVITAVAMADDIPVMV
                     IAIIIAVAVMMLAAKTIGDFVDNNPTIKNLALAFLILIGIVLVGEGFDIHIPKAAVYT
                     AMGFSVVVEMLNIKMRRNQAKLEQA"
     gene            complement(10959..11639)
                     /gene="mutH"
                     /locus_tag="PXA33_000906"
     CDS             complement(10959..11639)
                     /gene="mutH"
                     /locus_tag="PXA33_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005610420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HDL5120252.1"
                     /translation="MQLSTFSPTEQALLSKAEWLAGFTLGEIAEMLHIPIPADLKRDK
                     GWVGTLIETALGAKAGSKAEQDFAHLGIELKTIPVNQKGLPLETTFVSLAPLTQNNGI
                     IWETSHVKHKLSRVLWIPVEGERKIPLAERHIGQPILWSPTITQESRLQQDWEELMEL
                     IVLGELHKINATLGEVLQLRPKGRNNRSITSAINAKGEIVQSLPLGFYLRKHFTAEIL
                     QNFLHCPL"
     gene            complement(11731..12366)
                     /gene="lexA"
                     /locus_tag="PXA33_000907"
     CDS             complement(11731..12366)
                     /gene="lexA"
                     /locus_tag="PXA33_000907"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HDL5120253.1"
                     /translation="MRKHLTARQQEIFDFVKNHIETTGMPPTRVEIAREIGFKSPNAA
                     EEHLKALARKGYIEMLSGTSRGIRILVEDENQAANDEDEGLPLIGKVAAGTPILAVEH
                     VENHYPVNGAMFNPSADYLLKVNGNSMEKIGILDGDLLAVHKTNTARNGQVVVARVND
                     EVTVKRLEKKGELIYLHPENDELKPIVVNPAQEYIEIEGIAVGVIRSNAWM"
     gene            12533..14968
                     /gene="plsB"
                     /locus_tag="PXA33_000908"
     CDS             12533..14968
                     /gene="plsB"
                     /locus_tag="PXA33_000908"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HDL5120254.1"
                     /translation="MSGLLNFYRAFLNLPLSLLVKSRSIPTHPVAELELNLEQPIVYV
                     LPYTSQTDLLILQKNCLALNLPDPLQENVIEGQTLLRFVFLDEGRRFFKSKGAKSETE
                     SIFYRYLDLHRANAELDVQLVPVSVLWGRAPGKEDARHLQVLTSFQRFLSMVWFGRDN
                     FVRFSPAVSLRYMVTEYGADEKIAQKIARVAKMHFAKLRYSAMGPRLPNRDAMFNKIL
                     NSEVIQAAIAEEAKKSSPEKARKEAEKIINEIAADVKHESLRVADRVLSWLWNKLYQG
                     INVQNADRVRKLALEGHEIVYVPCHRSHMDYLLLSYLLYHQGLVPPHIAAGINLNFWP
                     AGPIFRSWGAFFIRRTFKGNRLYSTIFREYLAELFYRGYSVEYFIEGGRSRTGRLLEP
                     KTGMMSMSLQALQRGLNRSLSIVPVYIGYEHVLEVDTYAKELRGAAKEKENAGLVLRV
                     LKKLKNLGQGYVNFGEPILVNNYLNQYFPEWKEPSEDGRPKWLNEAVDNISHQVMVNI
                     NKAAAVNAKNLVGSALLASRQRALTREQLIEQLGSYIQLFRNVPYSKDMTLPTESAEV
                     MLNHVIHLPRSGVLIEKDNFGELVRLERESAVLMTYYRNNVQHLFVLPSLVASMVLHH
                     EAVSKDVVIKSVNRIYPFLQAELFLHFKQEELKAHIEAIIAELSRQGVIKNESDMLMI
                     NRSHIRTLQLHSAGVREILQRYYISLSVLLEQPNISRGYLEKESSSIAQRLSVLHGIN
                     APEFFDKAIFSTFTSSLKAQGYFDGDDNLAIAKVKETESLLRSLISIEVQQTIQGAMA
                     KVAESVENPNIAG"
     gene            complement(15073..15636)
                     /locus_tag="PXA33_000909"
     CDS             complement(15073..15636)
                     /locus_tag="PXA33_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005613534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HDL5120255.1"
                     /translation="MSAPNSGTNPAPLGLCGFALTTFLLSLINNGTFSGENVGLVLAM
                     GFAFGGTSQMIAGMFEFAKGNTFGFTAFTSYGAFWWSFALFKVFFAANVSAEFVGWYL
                     VAWGTFTLMMFIGTLAKARALQAIFLSLTITFYLLAAGDFTHNTTLTNIGGNFGLLTA
                     FLAFYLAAADIINESFGKTVLPIGEPK"
     gene            complement(15834..16730)
                     /locus_tag="PXA33_000910"
     CDS             complement(15834..16730)
                     /locus_tag="PXA33_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL5120256.1"
                     /translation="MDILDRLLAFAQISAGISVKCQLQGDWQLENPHQTGQAIAHIIS
                     YGQAYLQHNGKTHHLKQGDIVFFPRFADHTLRSQIQPLTSKNAVNPEQLGAFTLTQAG
                     LSQSECDMFCLHFYYDHQAELMESLPEMIVFQVAETPLKAMLDILKQEAETPNLAVKS
                     VVNSLAVVILTMILREHLVNHNNQLFGILKGWQDLHLRPLIKAILLNPEHEWQVDDMA
                     AFIHLSRAQLVRLFNKHLCISPHAFVHKIRLQKAAMLLKSQAGSVLSIALSCGFQSES
                     HFITAFKKQYCVTPSQYRQLNP"
BASE COUNT         4765 a         3292 c         3698 g         4984 t
ORIGIN      
        1 gactgtcaga tcaagtctga tcttctacaa ctaagccttc tcagaataaa taaattttca
       61 caagcggtgc aactttgcaa aaacttcggt attatgtagc agttgcacaa aaccccaaat
      121 tcattaaaac cgcttaaaaa tgaaatgccc taaactgatt tttcagtcta gggcatttat
      181 tatcacatat tatggttaat agggaaaact cacatcattg ttatgctggt gatatttttg
      241 ccaaaatgcg tgatattgtt ttttcaccga tgtgtttaat aaaaacggga ttatcaagac
      301 ataggtcggg caattccata gcattgcacg ttgtgtatcc gtgccgattt tggtgcgtaa
      361 gtaaccaaac tgatacatta gacccaatcc aaccgtcacc tattctaata gcataataac
      421 taaggtcgca tcattaaatt aaaagctcat tttttgcaaa aaatttccca aattccaccg
      481 cttgttggcg tacaataggc ataattttga tttacgatgg aggcagaatg tctgaaccga
      541 taaaacttac gcagttcagc cacggggcgg gttgaggctg taaaatttcg cctaaggtgt
      601 tagggacaat tttacaaact caattagaac aatttgtcga tccgaattta ttggtaggca
      661 atgaaacggc ggacgatgct gccgtctatg atttagggaa cgggcaggcc attatcagca
      721 ccaccgattt ctttatgccg attgtggacg ccccgtttga tttcgggcga attgctgctg
      781 ccaatgcgat tagcgatatt tatgcgatgg gtggcaagcc gattatggcg attgcgattc
      841 tgggctttcc ggtgaataaa ttgccggctg aagtggctca acaaattgtc gaaggtgggc
      901 gttttgcgtg ccagcaagcc ggtattgcct tggcgggcgg gcattcgatt gattcgcccg
      961 atccgatttt cggcttggct gttaccggca ttgtgccgac cgaacgggtg aaacgcaatg
     1021 cctctgcggt ggctgggtgt gagctgtttt taaccaagcc tttggggatt ggcattctaa
     1081 ccacggcaga gaaaaaaggt ttgttgaagc ctgaacatca acatctggca agcgaggtaa
     1141 tgtgccaaat caacttgatt ggttcagcgt tttccgaact gcctgaagtc acggcaatga
     1201 ctgatgtaac cggttttggc ttactcgggc atttaagcga ggtgtgtcaa ggctcgggct
     1261 tacgggcgga ggttgatttt gaggatattc aagtccttga tggcactaag gagtatattg
     1321 cattgggagc agttccgggt gggacaaatc gcaatttcga cagctatggg catttgattt
     1381 cggcaatgac ggacgagcaa aaagcgattt tgtgcgatcc gcaaacctct ggcggtttat
     1441 tagtggcggt gctgccacaa gcggtcgaaa aagtgcaaca aattgcaaat cgggcaaatg
     1501 tgccgttatt ccacgttggc agattgctgg aaatggaaca gggcaaacca cggattgagg
     1561 taatttgatg tttaaaccaa atgtaacaat ggcttgcgtg gtgcattgta agggaaagtt
     1621 tctgtttgtg gaagaaattg aatatggcaa acgtaccctt aaccagccgg cagggcattt
     1681 agaaaaagat gaaaccttgc tggagggggc aagccgtgag ctgtttgagg aaaccggtat
     1741 tcgggcagaa atggcaaatt tggtgaaaat ttatcaatgg caagcccctc gttcacaaac
     1801 cgatttcttg cgttttctgt tttcggtgga actggaagag tggctggagc cgaatccgca
     1861 agatgcggat attacccaag ccctttggct tactttagca gaatttgaag actacattca
     1921 gcaacaaggg caatgtgcca gaagcccgtt ggtggttcaa tcggtcaaag attatttaag
     1981 cggtgagcgt tatccattaa gtttgttgaa tgcgtttgaa tgggcgagaa atggaaaata
     2041 aaacaacaac ccaatttgat gtggtgattg tgggcggggc agtgaccggc tcggtgctgg
     2101 cattggcgtt aagtagtttt actcatcaca aaatctcgat tgcgatagtg gaaaaatcgc
     2161 tgccggacta tgaacagcaa ggcggttttg atgctcgcag tattgcctta gccgaaggca
     2221 gtttgcaaaa aatggggcaa atcagaccgc ttgcaggctc aagtttaggc gagatgatta
     2281 gcgaaatcgg cacgccgatt cagcaaattc aagtagcgga taaagggcat tttggtaaaa
     2341 cgaccttgaa agcaagcgag cagaatgtgg ctcaactggg tatggtggtg gaacttgcaa
     2401 aattcggcag aaaattgacc gcttgtcttg cacagcaagc taatattacg cttttttgcc
     2461 cgaatcaagt ggttggcatt gagcgttccc aacaacattg tggattgagc ttgaaaaatg
     2521 gtgagaaact aagctgttcc ttgctggtgg cggcagatgg tattcaatct cagattgcaa
     2581 aacagtgcgg tgtggaaacc gtaaaagtaa gggattatgc acaatcggca attatcgcca
     2641 atgtggaact gagtgaatct caccaaaatc aagcctttga atattttacg cctcaaggcc
     2701 cttttgcttt attgcctttg agcggaaata tgatgtcgtt ggtgtggtgt gtgaaagagc
     2761 cgaacgagtt aatccaactt tcagacagtg aatttttagc ccgtttgcaa caacaatttg
     2821 gttggaagtt agggcaattt gttcgagtga gcaagcggtt tgtttatccg ctcattttgc
     2881 aaaaagccca gtcgcatatt caccaccgct tagcagttgt cggcaatgcc gcacaaatgc
     2941 ttcacccggt tgctgggcaa ggtttcaact taggcttgcg tgatttgact accttagcgg
     3001 aattagtggc tttggcgttt aatcaaggga aagatatagg cgaattttcg ctcttatcaa
     3061 cttttgagaa tgagagaacg caagatcaag ccagaatgat aaatgctaca agcggtctga
     3121 tttcgctttt ttgttgcgaa atgttaccag ttcaggttgt gaggaattta gggttattaa
     3181 cggtttccca ttcaaaaata gccagagatt ttgttgctaa tcaggcatta ggttggtaaa
     3241 aaacacaaaa gcagcaattt tctatttgac actgttttta aaaaggcgta ttatccaacc
     3301 cgtttaaaaa tctcaccaaa gccttgaatg caaattcaag gctttttcct ttttagtgaa
     3361 agcctatgaa ttttgtttgc cgtaaaatcg gcaaaaaaat tttgcctaaa attcgcaatc
     3421 gtttgcgaaa taggtttata gcgttgtttt catctgggta actgcaatgg ttacatcgca
     3481 atacatcaaa gagagtaaaa aatgaccaca gaaaatatga gtacgccaaa gcaggcgttt
     3541 atcgggctgc aaatgctgtt tgttgcattt ggggcgttag tattagttcc gctgattacc
     3601 ggtttagatc ctaatgttgc ccttcttacc gccggtgtcg gtacgttaat cttccaaatt
     3661 tgtactaaac gtcaagttcc tgttttcctc gcatcgagtt tcgcttttat cgctccaatc
     3721 caattcggtg ttcaacaatg gggaattccc gtcacaatgg gagcattgct ctgttccggt
     3781 tttatttata tggcattatc tgctttagtg aaagcgaaag gggtgaatat tctgcataaa
     3841 tacttccctc caatcgtgat tggaccggta attatggtga ttggtttagg tcttgctcct
     3901 gttgcggtgg atatggcaac gggtaaatcg gcgaatattg cctataacac cgctattatt
     3961 gtttcaatga taacgctggt aactacgctg attgtggcgg tgtttgcgaa agggctatta
     4021 aagctcgtgc caatattagc aggtttaacg gtgggatata tcttgtctgt ttttatggga
     4081 ttggtggatt ttactaaagt ggttgaagct ccttggttta gtctaccgaa agtttccgca
     4141 ccggaattca aattagatgc aatcttgtat ttattaccta tcgcacttgc tccggcagtc
     4201 gaacacttag gcgatgtaat ggcaatcagc caagttgccg gtaaagattt tatgaaaaaa
     4261 ccggggttac accgcacgct tttaggtgat ggtttagcaa cttcagcagc tgcggcatta
     4321 ggtggcccac caaatacgac ttactctgaa gtaaccggtg cggtaatgct aaccaaaaac
     4381 ttcaatccga aaattatgac ttgggctgcg tgctgggcta tcgctttctc attctgcgga
     4441 aaaatcgggg cattccttgc cactatccct acttttgtga tgggcggtat tatgatgttg
     4501 ctgttcggtg cagttgcggt tgtggggatc aatactctaa ttaaagctaa agttgattta
     4561 agcattccac gtaatctttg tattgcttct gtagtaatga cattcggtat tggcggtatg
     4621 ttaattaaca tcggcgaatt ctcattaaaa gggatcagct tgtgtgcatt agtcgctatt
     4681 gtacttaaca ttgtattacc aaaagaaaaa gtagaagatt ctgctgctca ttaatcaaag
     4741 gacgctgtgg cgtccttttt gctaataacc ctctatttgt aaataaaaag gaataacact
     4801 atgcaaaaac tcacattttc accaccactt ttaaatacgc cttgcccttg gtgttcggag
     4861 ttagagagct tgcgtgaact ttatgcgtgt gaatataccg gtgcggtaac aacccgtact
     4921 tcaatgcctc aaccatatcc acacgattgg gcgaagaacc aatatgtact ctttgattcc
     4981 aacgcacaga aaacagcaag tgtgaatact caagatgcaa ctagcttaca aacagcaagc
     5041 cttaacacca tcggtttaag cccaaataat ctggatacca ccatcagctt tgtacgcacc
     5101 atttcaaatg agctgacagc gccatcaagc aaaccattta ttatttcggt gtttggttca
     5161 cccgaagagg tgggggaatg ttatgaaaaa attgttgctt tccaaactga agtgaaaatg
     5221 cctttggcaa tggagattaa tatctcttgc ccgaatattc cgggtgaaat ttcgcctgct
     5281 tattccgcag aagaattatc acattacttg cacgcactac aaacttcgct aaaaaaactg
     5341 gctcaaggtg ataatacttt accaatcggg attaaaattc caccttttac ttaccaaaat
     5401 caatatgatg aattaatcaa aggcttatta caagcggtcg aaaatggcga aaatttacca
     5461 atttccttta ttacttcaac caatacgctt ggctcatcgc ttgtcttaaa tgctggcaaa
     5521 gcggtattaa attcagaagt gggaacgggc gtaggcggaa tggcaggaac ggcaatccat
     5581 gcactttctt tggggaatgt ttacacgatt cgccaaatgt tagatcaaga gcctaagctc
     5641 aaacatcttc aaattattgg tgtgggcggc gtgaatgata aagccggctt tgagcgaatg
     5701 aaatcggttg gggcagactt tgtggggtta gctaccgcat taggggtgaa aggaatcggc
     5761 gtttttaaag agattctgtc tgaataatga agaggagggc gtgcattgca cgcccgaata
     5821 aatgaaatta gcctaaccat ttctccacta attcattagt ggtttcttta tgtacccaca
     5881 tactgccaag ttccggcatt gggaatttgg tgtggttgta gccgacaaat tggcggaaat
     5941 caaacacggc gtgcgggtag ccgttgatca gtaaaaaggc ttgatagccg tcttcactcc
     6001 agcaaatttc taaacggcga ggttctttta atgaattttg ctcaatatcg ctgacccgaa
     6061 acacaaataa actatccact accggctgtt cggtttggtg taagtccatt gcataaaagt
     6121 agccgctttc ttcatcaatt tcaaacatca ctactaaatg ttcgtgctgg gttgaatgag
     6181 cgccattacg gtaaggttcg ttcagtagaa tatgagcagt taaaacagag tagagcatta
     6241 aattatcctt ataagtaaaa tgccaccgat ttctcagtgg catttaaata tttaatcagg
     6301 caattacgct tgacctttaa tctctttacg gccgttgaac ggagccggtg taccagcacg
     6361 ctctaacgct tcttcgatac ggattaactg gttgtatttc gctacacggt ctgaacggct
     6421 cattgaaccg gttttgattt gacctgctgc tgtacctact gctaaatctg caatggttgc
     6481 atcttcagtt tcgcctgaac ggtgagagat aactgcggtg taacctgcat ctttcgccat
     6541 tttgattgca gctaaagttt cagttaaaga accgatttgg ttgaatttga ttaagattga
     6601 gtttgcgata cctttctcaa taccgttttt caagattttg gtattggtta cgaataagtc
     6661 atcaccaact aattgtactt tgtcgcctaa tactttagtt tggtatgcga aaccttccca
     6721 gtcagattcg tcttgaccat cttcgataga cacgattggg tactctttgc ataagccttc
     6781 taagtagtga gtgaactctt gtgaagtaaa ggttttgcct tcgcctttca tatcgtacac
     6841 attgcgttct ttgtcgtaga attcagatga tgcacagtcc atcgctaaag ttacgtcttt
     6901 acctaacaca tagcctgctt tttctaccgc ttctttaatg cacgctaatg cgtctgcgtt
     6961 tgaagccaag tttggtgcga aaccaccttc gtcacctaca gcagtattta agcctttcgc
     7021 ttttaatact tttgcaagat tgtggaatac ttcagcaccg atacgaagag cttcttttaa
     7081 tgtagaagca cctaccggtt gaatcataaa ttcttgaatg tccacgttgt tgtcagcgtg
     7141 ttcaccacca ttgatgatgt tcatcattgg taatggcatt gagtaaacac ccggtgtgcc
     7201 gtttaattca gcaatgtatg cgtaaagcgg taagcctttt gaagcggctg ctgcttttgc
     7261 tgttgctaaa gaaaccgcta agatagcgtt tgcaccgaat tttgatttgt tttctgtgcc
     7321 atctaaatcg atcataattt ggtcgatttc agcttgtgca gaaccgtctt tgcctactaa
     7381 tgcgtttgca atttcgttat ttacggcaga aaccgctttt aatacacctt tacctaagaa
     7441 acgacctttg tcgccatcac gtaattctaa tgcttcacgt gaaccggtag aagcacctga
     7501 tggagcggct gctaagccta caaaaccacc ttctaagtga acttctgctt ctacagttgg
     7561 gttaccacga gagtcgatga tttcacgacc gattacttta acaattttag ccattgttga
     7621 tgtcctctaa tatacgagtt aatagaaaaa agaataggta tatagtaaag cgattttctg
     7681 cttttgtctt ggaaaaactg attgagtggc agaaaatttg ccactaattt gatctaagac
     7741 tatattcgcc tgagattata gcaacctttg caaaaaacgg caaaaatttg cccgcttgta
     7801 atgcttattt tttgactaaa aaagtcaagg tttcataatg tgctgtgtga gcaaacatat
     7861 caaacagctg cactttgtgt agttcgtaat ttgtcaaaaa tgtaaaatcg ttcgccattg
     7921 agacggcatt acaactggag taaatcaaat aaggtgagcc taattgattt aaaaattctg
     7981 ctaatagttt gccgatacca cgtcgaggtg ggttcacaat cactaaatcg ggtggagaac
     8041 cagcttcatt taaagcaaat tgtgcagaat ctaatgaggc aaattttacc tgctttaggc
     8101 cgagtttttc agccgatttg gtcgcacttg caatagctga ggctgaaatc tcaatgcctg
     8161 tcagctccac gttcgagttc ttcgcttgca aggcgttggc acaatgtaag ccgaaaccac
     8221 cgacaccgca gaacaagtcc cacaatttag caataggtaa atgctcaatc cagctttgtg
     8281 ctgtgtggta aagctggctt gctaccacag ggttagtttg gaaaaagccc tgagggcgaa
     8341 taaatagcgg tatgccgtta aaatgttctt ccagcacttt ttgttcggtg aggaaaatct
     8401 cagtttcgcc ttctaaaaga gcagcgtgtt ggggctgaat attcagacta actactgagt
     8461 tgggtggcag tttttccaac agttcgggca attcccgttc aaccaatgca cgctttgctt
     8521 cactacgaac cacaaaacgg agcataaatg attgattata ttggctttcg gtaatcagaa
     8581 tatattttag ctcacctttt ttcttactga tgttatacgg cactaaacca gctcgagcga
     8641 taaaatcttt caaaatcgga aaaagtctat gaaatgagga cgggtagagc agacagtcgg
     8701 ttaaatccac cccgctttga gcatcatttt gatcttttaa aatgcctaaa atcgggcgtt
     8761 ctacactgcc ggagaccacc attttcgctt tgttacgaaa ttgcgaaatt ggcgagccga
     8821 caagcggtaa gatttccgtt ttattttgca aaataaacgg caaaatcaac cgcttgagat
     8881 cggcctcttt ggcggctaat tgcgtgctgt atggcttttc aatccactgg cacgaagtac
     8941 agtcgccacg ctcaaaatgc gaacaatgca acaagctaga cattcgcttc cttccattcc
     9001 agccagttgt tttgtaaggc cgttaattca gcctgagttt gttggtagcg agagctgttt
     9061 tccagctctc gccaagtcac ggttttgcgt ttcattaaag aaaggcgacg ttctttttgt
     9121 gcttgagtcg gctgatattt cagcccgtct aacacttgca ccaggccttc aggctcgtta
     9181 caaaggagca ataaatcaca gccggcttgg attgcttttt cagaacgctc gacaaaattg
     9241 cccataaagc ccgcaccttt cattcccaaa tcgtcagaga atatcacacc gttaaagttc
     9301 agctgtttac gcaatacctc tttaagccaa tagcttgaac cgcttgccgg ctggctatca
     9361 cattgggtgt aaacgacgtg ggcaggcata atggcagaga gttttccttt ggcaataagc
     9421 tgtttgaacg gcagaatatc gtgattaaaa atccgctctt tcgggcgatc atcaaacggg
     9481 gtttctaaat gcgaatcggc taaaacgtgt ccatgaccgg ggaagtgttt gccggtggtc
     9541 gccataccga tttcacgcat tccgtcaata aaggcttcgg caacaggcaa aatcgctgct
     9601 ggctcttgcc cgaatgagcg atcgccaatc gctttgcatt cgtgtcctaa atccagtacc
     9661 ggagcgaaac tgaggtctat atcgagggca aacatttctg ctgccatcaa ccagccggtt
     9721 tctttcgcta attgcattgc ttgctctggc gttgtggcaa gttggttaaa agattgcatt
     9781 gccggcagtt tagtaaagcc ttcacgaaag cgttgcaccc gaccgccttc ttggtcgaca
     9841 gtaataagca acggtttttt cacccgctgg cgaatggatt tcactaatgc ctgaatttgt
     9901 gctttgtcgt agaaattacg ggtgaataaa attagcccgg aaactaaagg gtgttccagc
     9961 atttcgactt cttcttgagt cagttcttta tctttaatat caatcagtaa cataattttc
    10021 gttgagctat ttgcaaaaaa atgagcagat atgaccgctt gttgtgccta ttctacatta
    10081 aaaagccctt gatgttgatc aagggcgaat gattaagggc ataaatgaga cagagcaatt
    10141 atgcttgttc taatttagct tggttacgac gcatttttat gttgagcatt tcaaccacaa
    10201 ccgagaagcc cattgccgta taaaccgctg cttttggaat atgaatatca aaaccttcgc
    10261 cgactagtac aataccgatt aaaatcaaga acgctaaagc gagattttta atggttgggt
    10321 tgttatctac aaagtcccca atggttttcg ctgctaacat cattaccgca actgcaatga
    10381 taatcgcaat aaccattacc ggaatatcat ctgccattgc cacagcagta atgactgaat
    10441 caagggagaa aataatatca aaaattgcaa tttgaattaa caccataaaa aagctggctt
    10501 ttttaatgga ttcgcttttt tcttcgtgag attcggcaga aatggattct ttcagctcca
    10561 ttgcactttt aacaatcagg aataatccac cgatgagtag aatgagatct ctacctgaaa
    10621 ttggattgtt gccaatagtg aaaagaggct cgaccaaacg catcatccaa gcaagcgaga
    10681 gaagcaataa aatacgggtt cccattgcca gccccaagcc gataattcgg gcagattggc
    10741 gttgttcaat aggtaaacgg gaaacgagaa tactgatgac gatgatgtta tcaatgccta
    10801 atacgatttc aagcccggta agtgtaaaga gcgcaaccca agcttcagga tttgtgatcc
    10861 agtcaaacat aaaaatgtcc ttttaaaaat aagggattaa gtgatatttt ctatcaaata
    10921 gaattaaagc cgacatctta tagagtgtga ggacaaattt aaagggggca atgcaaaaag
    10981 ttttgtaaaa tttcggcggt aaaatgttta cgcaaataga aaccaagagg gagagattga
    11041 acaatctccc ctttagcgtt gatagcagag gtgatagagc ggttatttct acctttgggt
    11101 ctgagttgta acacttcgcc taaggttgca ttaattttat gcagttcacc taacacaatc
    11161 aactccatta attcctccca atcttgctgt aggcgggatt cttgtgtgat agtcggagac
    11221 cataaaatcg gctgaccgat atggcgttcg gcaaggggta tctttcgttc cccttcaacc
    11281 ggaatccaaa gcacacggga aagcttatgt ttaacgtggg aggtttccca aataataccg
    11341 ttgttttggg ttaaaggagc aaggctgacg aatgtggttt ccagcggtaa tcctttctga
    11401 ttgaccggaa tggttttcag ctcaatgcct aaatgtgcaa aatcctgttc ggctttactg
    11461 ccggctttcg cacctaaagc ggtttcaatc agagtaccga cccagccttt atctcgtttc
    11521 agatctgctg gtatcggaat atgaagcatc tcggcaattt cacctaaagt gaaccctgcc
    11581 agccattccg ctttgcttaa taatgcctgc tcggttggag agaatgtaga taattgcatt
    11641 gcacttagtt aattagacta aactggcaag cggtcaaatt taggcagaaa tttgcaaaaa
    11701 attactaaaa aataaccgct tgtgaggaaa ttacatccaa gcattactgc gaataacgcc
    11761 tactgcaatg ccttcaattt caatatattc ttgagcaggg tttactacaa tcggttttaa
    11821 ttcatcgttt tcagggtgta agtaaattaa ttcacctttc ttctctaagc gtttaacagt
    11881 cacttcatca tttacccgag caaccaccac ttgcccgtta cgagcggtat tggttttatg
    11941 aacggctaat aggtcaccgt ctaaaatacc gattttttcc attgaattgc catttacttt
    12001 gagtaaataa tcagcacttg gattaaacat cgcaccattc accgggtaat ggttttcaac
    12061 gtgttcaacc gctaaaatag gtgtgccagc agctactttg ccaatcaacg gtaagccttc
    12121 atcttcatcg ttggcagctt ggttttcgtc ttcgactaaa atgcgaattc cacgagatgt
    12181 acctgataac atttcaatat agcctttacg agcaagggct tttaagtgtt cttctgccgc
    12241 attaggtgat ttaaagccga tttcacgggc tatttctact cgggttggtg gcataccggt
    12301 ggtttcaata tggtttttga cgaaatcaaa aatttcttgc tgacgggcag ttagatgttt
    12361 acgcatttta agttaatcct cactgtcttt atatacagaa aatattgata gtatatacag
    12421 tatttttgat tttgtaacta ataatttcac gaaattccat aaaaatttat ggtagaatgg
    12481 aagaaattag aggttagacc tccaacaata acgattctaa aaggatattt atatgtctgg
    12541 cttattaaat ttttatcgag ctttcctaaa cttaccactt tcacttttag taaaatctcg
    12601 ctctattccg actcatccgg tggctgaact ggaactaaat cttgaacagc cgattgtcta
    12661 tgttctacct tatacctcgc aaaccgattt attaatttta caaaaaaact gcttggcttt
    12721 gaatttgccc gatccgttgc aagaaaatgt gattgaaggg caaacattgc ttcgttttgt
    12781 atttttagat gaaggtcgcc gtttttttaa atctaaaggg gcgaaaagtg agacagagtc
    12841 gattttttat cgttatttag atttacatcg tgctaatgct gaattagacg tccaattagt
    12901 gccggtttcg gtattatggg ggcgggctcc gggcaaagaa gatgcccgcc atttacaggt
    12961 gcttacctca ttccaacgtt ttttatccat ggtttggttt gggcgtgata attttgtacg
    13021 tttctcccct gcagtatcgt tacgctatat ggtaactgaa tatggtgcag atgaaaaaat
    13081 cgctcaaaaa atcgcgcgtg tggcgaaaat gcactttgca aaattgcgtt attcagcaat
    13141 ggggcctcgt ttaccaaatc gtgatgcgat gtttaataaa atcctgaatt cagaggtgat
    13201 tcaagctgcg attgcggaag aagcgaagaa atcatcgcct gaaaaggctc gcaaagaagc
    13261 cgagaaaatc attaatgaaa ttgccgctga tgtaaaacac gagagtttgc gtgtagcaga
    13321 tcgagttctg agctggcttt ggaataagct ttatcaaggg atcaacgtgc aaaatgcaga
    13381 ccgtgtgcgt aaattggcat tagaaggtca cgagatcgtt tatgtgcctt gccaccgtag
    13441 ccatatggac tatttgctcc tatcttatct actttaccat caaggattag taccgccaca
    13501 tattgccgct gggatcaatt taaatttctg gccggcaggc ccgattttcc gtagttgggg
    13561 agcatttttc attcgccgta cctttaaagg aaatcgcctc tattccacca ttttccgtga
    13621 atatttagcg gaattattct atcgtggtta ttcggtcgaa tattttattg aaggcgggcg
    13681 ttcacgcaca ggtcgtttat tagagcctaa aaccggtatg atgtcgatga gcctacaagc
    13741 cttacagcga gggctgaata gatctttaag tattgtgcct gtttatatcg gttatgaaca
    13801 cgtcttagaa gtcgatactt acgctaaaga gttgagaggt gcggcaaaag agaaagaaaa
    13861 tgcgggcttg gtgctacgag tgctgaaaaa actgaaaaat ttaggtcaag gatatgtgaa
    13921 tttcggtgag ccaattttgg tgaataatta cttaaatcaa tatttccctg agtggaaaga
    13981 gccttccgaa gatggacgac ctaagtggtt aaatgaagcc gtggataata tttcacatca
    14041 agtaatggtg aatatcaata aagcggcagc agttaatgcg aaaaacttag tggggtcagc
    14101 attattggca tctcgccagc gtgcattaac ccgtgaacaa ctcattgaac aacttggaag
    14161 ttatattcag ttatttcgta atgtacctta ttctaaagat atgacattac caactgaaag
    14221 tgcagaagtg atgttgaatc atgttattca tttacctcgt tcgggcgttt taattgaaaa
    14281 agataatttt ggtgaactgg ttcgtttaga acgagaatct gctgtgttaa tgacatatta
    14341 cagaaataat gtgcagcatt tatttgtgtt gccttcactt gttgcaagta tggtacttca
    14401 tcatgaggct gtttcgaaag atgttgtgat taagtcggtt aatcgtattt atccattctt
    14461 gcaagcagag ttattcttac actttaagca agaagaatta aaagcacata ttgaagcaat
    14521 tattgcggaa ctaagtcggc agggggtgat taaaaacgaa agtgatatgt taatgatcaa
    14581 tcgctcacat attcgtacct tgcaattaca ttcagctggt gtgcgtgaaa tccttcaacg
    14641 ttattatatt agcctaagtg tcttgttgga gcagccaaat attagtcgtg gctatttaga
    14701 aaaagagagt agttcaattg ctcagcgttt atcagtcttg catggtatta atgcgccgga
    14761 gttttttgat aaggcgattt tctctacctt tacatcaagt ctaaaagcac aagggtattt
    14821 tgatggtgat gataatttag cgattgcaaa agttaaagaa acggaatcgt tgttacgtag
    14881 tttgatttcg attgaagttc aacagaccat tcaaggtgcg atggctaaag tcgcagaatc
    14941 tgtagagaat cctaatatcg caggataata aatcaaagaa gggcgctaac aagcgctcgt
    15001 ttcttgcaat tttgttgcca aaaataaaag ctgaatgcgc gaccacattc agctttttta
    15061 cttcctatat aattattttg gttcaccgat tggtaaaaca gtcttaccaa aactttcatt
    15121 aataatatcc gcagcggcta agtagaacgc taagaatgcg gttaataaac cgaagttacc
    15181 accaatgtta gttagggtgg tgttatgggt aaaatcacct gcggctaaaa ggtagaaagt
    15241 aatggttaaa cttaagaaaa tagcttgtaa tgcacgggct tttgctagtg tgccgatgaa
    15301 catcataagt gtgaatgttc cccaagctac taagtaccaa ccaacgaatt ctgccgatac
    15361 attcgcagca aagaacactt taaataatgc aaaagaccac cagaatgcac catagctggt
    15421 aaacgcagta aagccgaacg tgttaccttt agcaaattca aacatacctg caatcatttg
    15481 tgatgtgcca ccgaaagcaa atcccattgc taaaactaaa cctacgtttt caccgctgaa
    15541 tgtaccgtta ttgattaggc ttagtaaaaa tgtagtaagt gcaaaaccgc ataaaccgag
    15601 cggagccggg ttagttcctg aatttggagc tgacattgaa aacctctgtt atattgttac
    15661 accataagtg tggtaattgt ttatttacgc cgataaatca gacgttatgt gcctaaaatt
    15721 tagggcgata aagtatagcg attttgcagt aaattgacta gaaatttccg agacaagcgg
    15781 tgagattttt gcaaaatttt gccaaaaaat gaccgcttgt tgttgtcatt tagctatgga
    15841 tttaactgcc gatattggct gggtgttacg caatattgct ttttaaaggc ggtaataaaa
    15901 tgcgactcgg attggaagcc gcaagataag gcgatactta ataccgaccc agcttggctt
    15961 ttgagcagca ttgctgcttt ttgtagccta attttgtgta caaaagcatg tgggctaata
    16021 cataaatgtt tgttaaataa gcggactaac tgggcacgag aaaggtggat aaatgccgcc
    16081 atatcatcga cttgccattc gtgttcggga ttaagcaaaa tagctttgat caaaggtctt
    16141 aagtgtaagt cttgccaacc ttttaaaata ccgaataact gattgttatg gttaactaga
    16201 tgttctcgta agatcatagt taaaataaca acagcaagcg agttaaccac agattttacg
    16261 gctaaattag gtgtttctgc ttcttgtttt agaatatcta acattgcttt taatggtgtc
    16321 tcggcaactt gaaatacgat catttcaggt aagctttcca ttaattcggc ttgatggtca
    16381 taataaaaat ggaggcagaa catatcgcat tctgattgag aaagtcctgc ttgggtgagt
    16441 gtaaatgcac ccaattgttc agggtttacc gcatttttgc tggtcaaagg ctgtatttga
    16501 ctacgtaatg tatgatcggc aaatctgggg aaaaatacaa tatctccctg tttaaggtga
    16561 tgggttttcc cattatgttg tagataagct tgcccatagc tgatgatatg agcgattgct
    16621 tgtccggttt gatgcggatt ctcaagttgc caatctccct gtaactgaca tttcacactg
    16681 attccggctg agatttgagc gaaagctaga agcctatcta atatatccat tgttcattc
//
LOCUS       DANLRK010000039        16580 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.039, whole genome shotgun sequence.
ACCESSION   DANLRK010000039 DANLRK010000000
VERSION     DANLRK010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 16580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16580
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.039"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(60..2162)
                     /gene="fusA"
                     /locus_tag="PXA33_000911"
     CDS             complement(60..2162)
                     /gene="fusA"
                     /locus_tag="PXA33_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDL5120257.1"
                     /translation="MARTTPISLYRNIGISAHIDAGKTTTTERILFYTGVSHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMSQQYPQHRINVIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRTGANFLRVVEQIKT
                     RLGGNSVALQLPIGAEDSFKGVVDLIKMKAINWNEADQGMTFTYEDIPADMLEACEEH
                     RAMLVEAAAEASEELMEKFFSGEELTEDEIKAALRQRVLAGEVIPVCCGSAFKNKGVQ
                     AMLDAVIDYLPAPTDIPAIKGINEDETEGERHASDDEPFASLAFKIATDPFVGNLTFF
                     RVYSGVINSGDTVYNSVKQKRERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVGTG
                     DTLCAIDAPIILERMEFPEPVISVAVEPKTKADQEKMGLALGRLAQEDPSFRVHTDEE
                     SGETIISGMGELHLDIIVDRMKREFKVEANIGKPQVSYRETIRTRVNDVEGKHAKQSG
                     GRGQYGHVVIDLYPLDAEGPGYEFVNEIKGGVIPGEYIPAVDKGIQEQLKSGPLAGYP
                     VVDLGVRLHFGSYHDVDSSELAFKLAASLAFKAAFAKASPVLLEPIMKVEVETPPDYV
                     GDVIGDLSRRRAMVNGQEANEFVVKINAEVPLSEMFGYATDLRSQTQGRASYSMEPLK
                     YAEAPKNVADAIIEARRK"
     gene            complement(2264..2734)
                     /gene="rpsG"
                     /locus_tag="PXA33_000912"
     CDS             complement(2264..2734)
                     /gene="rpsG"
                     /locus_tag="PXA33_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007526570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HDL5120258.1"
                     /translation="MPRRRSIEPRKILPDPKFGSELLAKFINVLMVDGKKSTAEKIVY
                     GALETLTQRSGKEALEAFEIALENVRPTVEVKSRRVGGSTYQVPVEVRPTRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAADNKGSAVKKREDVHRMAEANKAFAHFRW"
     gene            complement(2865..3239)
                     /gene="rpsL"
                     /locus_tag="PXA33_000913"
     CDS             complement(2865..3239)
                     /gene="rpsL"
                     /locus_tag="PXA33_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HDL5120259.1"
                     /translation="MATINQLVRKPRVKKVVKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRIRLTNGFEVTSYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHTVRGAL
                     DCAGVKDRKQGRSKYGVKRPKA"
     gene            complement(3360..4319)
                     /gene="rfaC"
                     /locus_tag="PXA33_000914"
     CDS             complement(3360..4319)
                     /gene="rfaC"
                     /locus_tag="PXA33_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HDL5120260.1"
                     /translation="MKILIVKTSSMGDVLHTLPAITDMQNAIPDLKVDWVVEENFTEI
                     PAWHSAVNKVIPVAIRRWRKNLCQRQTWVEWQQFLKQLKSEQYDAVIDAQGLIKSAVL
                     VTRQAKGKKFGYDKNSAREGLSSLFYDETFNIPYQQHAVERIRKLCAKSLGYALPTER
                     GDYCIAQHFAKNPQKTTAYIMLIHATTRADKHWQEAYWAELIQALDKHNLAVRLPWGN
                     QAEKERAERLASVANNAKVLPKMSLTDIATQLAGAAAVVSVDTGLSHLAAALDKPNVI
                     LYGATDPKLIGAYGQNQFYLKADKMENITSSQVLEKLLPLINK"
     gene            complement(4386..5423)
                     /gene="waaF"
                     /locus_tag="PXA33_000915"
     CDS             complement(4386..5423)
                     /gene="waaF"
                     /locus_tag="PXA33_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HDL5120261.1"
                     /translation="MNILVIGPSWVGDMMMSHALYQQLKIQYPNCQIDVMAPDWCCPL
                     LARMPEVRNAISMPIGHGSFRLCERYRLGKSLRNQYDMAIVLPNSLKSAFIPLFAKIA
                     RRRGWKGESRYFFLNDLRSNKNDYPMMVQRYVALAFEKATVPTAQQLPIAYPYLTTDS
                     EQIEQTKAKFEKQLAYAENRPAIGFCPGAEFGPAKRWPHYHYAILAKMLIEQGYSVRL
                     FGSKKDEAAGEQIRLALPENLQRYCLNLAGQTDLNQAVDLIADCNAVVSNDSGLMHIA
                     AALNKPLVALYGPTSPQYTPPLSQNAEIMRLIEGGLIKIRKGEGAEGYHQSLIDIQPE
                     MVMDKLAELLV"
     gene            complement(5534..5896)
                     /locus_tag="PXA33_000916"
     CDS             complement(5534..5896)
                     /locus_tag="PXA33_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="HDL5120262.1"
                     /translation="MLVACSTVVTPVEQIHQVQKEKESVKKVTKLIAQAPKVSLYRCE
                     NDKKVEVERQGRTKKALKKEIVTVSYQGTTHQLSPSVTRDGKKYTNIRWTWHETRSGK
                     AFLYNNTKKTLAANCIKQ"
     gene            complement(6014..7333)
                     /locus_tag="PXA33_000917"
     CDS             complement(6014..7333)
                     /locus_tag="PXA33_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="opacity-associated protein A"
                     /protein_id="HDL5120263.1"
                     /translation="MTQNNFRKEPVFGESSVTSQDAEATKTDTAQAPYVSLRSTKAPG
                     HTFTPIMKRPDAKVEDHTADVFSAKQAKEFQFTPSSEATEAVEPDNTIHSVEESVSTA
                     SFNAVEAQENVEKEVVKPAPESFTTERIIPNAATAAAAATAAAAATKTKEIKAKMPPK
                     TRRLLLVALLALALLVLFFLLKPSTPETVEELQSQQGGSLPIEFRPVDEAEAKRAEEQ
                     ARAEQEAAEAAKAQEQAQAAQQESVTVAPAQTEQTMAKPSQDSQSQTQSQAVVEQPVA
                     VKPVENKPVVAQPAIKPKTENSVVFQPETTVKQQPKAPAKTEVKTERATLAAKETAKT
                     AAKPVEKAPTKTEAVKAAPAAESANAVATKTMTVPKGVSLMQVFRDNNLNISDVNAMS
                     KVNNVVSNLKVGERVTVRLDKNNRVVEMSIGSGGKFTRQANGSYTFK"
     gene            7626..7964
                     /locus_tag="PXA33_000918"
     CDS             7626..7964
                     /locus_tag="PXA33_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HDL5120264.1"
                     /translation="MKKIKQLGLTFLFGLNAMAFAQTPPTTMPVEQNAVTQPVQQTQI
                     TNANAVNINTASAAELQDKLVGIGQKKAQAIVDYRTKNGNFKNIEQLAEVSGIGQATV
                     EKNRANIILE"
     gene            8129..9022
                     /gene="psd"
                     /locus_tag="PXA33_000919"
     CDS             8129..9022
                     /gene="psd"
                     /locus_tag="PXA33_000919"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251643.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HDL5120265.1"
                     /translation="MQLKKYPVPTYFQRVKIALHYLLPQLAITRAAGWLAEQKWGVVT
                     HLIIKLFAKQYKVNLSEAKKTEPSDYATFNEFFIRELKENARPINQDPNTLCLPAEGK
                     VSESGEISDNRLLQAKGHHFTLETLLANDEEMAAKFKDGTFITTYLSPTDYHRVHMPC
                     DGTLRKMIYVPGELFSVNPFLAEHVPNLFARNERVICEFDTAFGPMLQILVGATVTAS
                     MSTVWAGVINPPRADEVVVWNYETEGEKAIKLQKGEEMGAFRLGSTVINLFPKGKVHL
                     NPALIAGMQTRMGEELGRIIN"
     gene            9094..11445
                     /locus_tag="PXA33_000920"
     CDS             9094..11445
                     /locus_tag="PXA33_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDL5120266.1"
                     /translation="MYTLQELKQMNEQEIRSRLISPAIRNAGWSDRQIGEEYTFKTNK
                     RFTDGQVVVDPKTTQTKRIEAKRVDYLLYTSANQKIAVVEAKDNHHSSRHGLQQAMTY
                     ARLLDVPFAYSSNGDEFVEHDFITGVQRTLPMSAFPTPDELHQRWAKQYTTEELKIIQ
                     QPYYTSSTDTFKEPRYYQEIAINRTVEAVAKGQQRILLVMATGTGKTYTAFQIIHRLK
                     TSGAKKKILFLADRNVLVDQPMMQDFKPFKDKMVKVSNKKLDSAYEVYLALYQQLDGE
                     TDNELFKQFQPSFFDLIVIDECHRGSAKEDSDWRAILKYFKDATHIGMTATPKETDDA
                     SNITYFGEPIYTYSLKQGIEDGFLAPYRVIRVDLDCDLKGWRPTKGLKDHQGNEIEDR
                     LYTVKDFDYNIVLNDRTRLVAERICEFLRKTDRLQKTIVFCANINHAERMVAELSRLN
                     ADMLKHNSKYVCQITGDQSGKGVDLEEFCDVANEGKDYPVIAVTSKLMTTGVDSKTCK
                     LVVLDANIQSMTEFKQIIGRGTRLRPDFGKEFFTILDFRGVTRLFADPAFDGEPVVIL
                     DVPENKPLVEQPLPKPSDKDIIKDPELKPYKIPVVVDFGTLKLRILDEKVQYLNEQGE
                     LVSEDIDLFSKREMQKEFGSYEQFQQQWTTSGEIFSKFYTDPKWLAALRQTRQFSHDV
                     EDFDVLSHISFGKKPLTKTERAEKVKQSGYVEQYSPENQQVLGLLLNEYVKSGNQDLT
                     KTEILKTPQFSRFGGLVSIIKNFGGKELYQKAVKGLEKMIYSE"
     gene            11493..12962
                     /locus_tag="PXA33_000921"
     CDS             11493..12962
                     /locus_tag="PXA33_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HDL5120267.1"
                     /translation="MSLNNIVKRLQDVMRNDAGINGDAQRIEQIVWILFLKIYDAKEQ
                     EWELENDEYHSILPNFLRWQNWAEDKKDGKAMTGDELLSFVNNELFPTLKNLPISADT
                     PMNQRIIRAAFEDNNNYMKNGVLLRQVINIIDEIDFAHYQERHAFGDIYENILKSLQS
                     AGNAGEFYTPRAVTDFMVQAIKPKLGERIADFACGTGGFLTSALKVLESQIQTLSDRT
                     LFNNSVYGIEKKALPHLLCITNLLLHDIDNPNVHHDNALEKSVKDYTENDKFDVILMN
                     PPYGGSEIEQIKKNFPTALQSSETADLFMSVIMYRLKQNGRAAVVLPDGFLFGTDNAK
                     VAIKKKLLEEFNLHTIIRLPHSVFAPYTSITTNILFFDKTEPTRETWVYRLDMPQGYK
                     NFSKTKPMKLEHFNEVVEWWGNRQVIEVEGFDKAKSFTLKEMSDRQYNLDLCGFPHEE
                     EEILPPDELIADYQRKRTAYNADIDKILAEITDILGIKL"
     gene            12975..14636
                     /locus_tag="PXA33_000922"
     CDS             12975..14636
                     /locus_tag="PXA33_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HDL5120268.1"
                     /translation="MLTWEHIEKMIAHQQFIREKAEIEYKEAKNSVPKDLWHTYSAFA
                     NSNGGFILLGIKENEQGHFPELLISGVNDPDKVIDELFSQSRSEKVSKSLLKDNDVKI
                     FQVENKSVIAIYVKAADILDRPIHLNGDYRLSYVRLNSGDHKLTSSELRSFISSYSQK
                     DADGRIVPYASIDDISLSTLDKYRNLLKAHNSTSKLLTLSDEKLLQEIGAYKKDLAQN
                     SEGITNAGLLMFGKHYAITSIFPHFFFEYYEEAGDVKRYTLRITDFDLEEGNLFEFYL
                     KIVPLIQELGKDKHFKLDNLTRTEENEITDGLREALINAITHADYFNNVRHLKVIKTK
                     DNKLIFENAGVMLVDIPTAIAGQRSECRNSIIHNLFHRLGLCERQGQGVRDIYSYWQS
                     RNFNSPLLESEIDYTKLTLTFQSGMIADFEEKFTACFGENFSELQPIQKDCLLFIAFN
                     QGKAMFAELAQKLPQYPRRNITLALARLKEKGLLTSSGSPRKQLYTLVNLEWIITEKS
                     QDDYFNPPFDQLDKASESKGAPPKKSKVKQVKQLPQNKANKLPPK"
     gene            14842..16362
                     /locus_tag="PXA33_000923"
     CDS             14842..16362
                     /locus_tag="PXA33_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDL5120269.1"
                     /translation="MKAQQLKNAILQLAIQGKLVPQDPNDEPASELLCKIQAEKDRLI
                     AEGKIKKNKKTADKAPYTIEPPFEIPESWVWVRLGDICLKITDGTHHSPPNIDKSDFL
                     YITAKNIKKDGLDLSKISYVTKEIHNEIFSRCNPEKGDILYIKDGATTGVSIINTLNE
                     PFSMLSSVALIKTSQEIDNEYLNYVMNSHYFYNISIGSMSGTGIPRITLTKLESYLVP
                     VPPLLEQQRIVQKIEELLPLVERYEQTEQQLTKLNNTFPEQLKKSVLHAAIQGKLTEQ
                     DPNDELASCLIERIKAEKNRLIAEKKLKKPKSVSEIVMRDNLPYEIKAGQERCIADEV
                     PFEIPQNWTWVRLGEVGDWSSGATPNRHNPEYYNGTIPWLKTGDLNNGIISSIPEFIT
                     DKALQECSVRLNPVGSVLIAMYGATIGKLGILKIAATTNQACCACIPFNGIYNKFLFY
                     YLMSQKAEFQKKSEGSGQPNISKEKIINYLFPLPPIHEQHRIVQKIEQLFAEIEKL"
BASE COUNT         5047 a         3424 c         3211 g         4898 t
ORIGIN      
        1 tttaatatct tcctaaaaaa tgagccatag ccctattgct atggctcagg ttaaacaaat
       61 tattttctac gagcttcaat aattgcatca gcaacgtttt tcggtgcttc agcatatttt
      121 aaaggttcca tagagtatga tgcacgacct tgagtttgtg aacgtaagtc tgttgcataa
      181 ccaaacattt ctgaaagtgg aacttcagca ttgatcttaa caacgaactc gttcgcttct
      241 tgaccgttaa ccattgcacg acgacggctt aagtcaccga ttacatcacc cacgtaatcc
      301 ggtggagttt caacttccac tttcatgatt ggctcaagta ataccgggct tgcttttgcg
      361 aacgccgctt taaatgctaa tgatgcagca agtttaaacg caagttcaga tgagtcaaca
      421 tcgtggtatg aaccgaagtg taaacgcaca cctaaatcta ctaccgggta acctgctaac
      481 ggaccagatt taagctgttc ttggatacct ttatcaaccg caggaatgta ttcaccaggg
      541 attacaccac ctttgatctc gtttacaaac tcataacccg gaccttcagc atctaatggg
      601 tataagtcga tcacaacgtg accatattga ccacgaccac cagattgttt tgcgtgttta
      661 ccttcaacat cattaacacg agtacggata gtttcacggt aagatacttg tggtttaccg
      721 atatttgctt ccactttaaa ctcacgtttc atacggtcaa cgatgatgtc taagtgtaac
      781 tcacccatac ctgaaatgat cgtttcacca gattcttcat ctgtgtgaac acggaatgaa
      841 gggtcttctt gtgctaaacg acctaaagct aagcccattt tctcttggtc agctttggtt
      901 tttggttcaa ccgcaacaga gattaccggc tcagggaatt ccatacgctc aagaatgatt
      961 ggtgcatcga ttgcacataa tgtgtcacct gtaccaacat cttttaaacc gattgctgca
     1021 gcgatatcgc ccgcacgaac ttctttgatc tcttcacgtt tgtttgcgtg catctgtacg
     1081 atacgaccaa aacgttcacg tttttgtttt actgagttgt aaactgtatc accagagtta
     1141 attacaccag agtaaacacg gaagaaggtt aagttaccta cgaatgggtc tgttgcaatt
     1201 ttgaatgcta atgaagcaaa tggctcatcg tcgcttgcgt gacgctcacc ttcagtttca
     1261 tcttcattga tacctttaat tgcaggaata tcagttggtg ccggtaagta atcgattacc
     1321 gcatcaagca ttgcttgtac acctttgttt ttaaatgcag aaccacagca aacagggatt
     1381 acttcacctg ctaatacacg ttgacgtaat gctgctttga tttcatcttc agttaattct
     1441 tcaccagaga agaatttttc cattaattct tcagaagctt cagctgctgc ttcaacaagc
     1501 attgcacggt gttcttcaca agcttcaagc atatctgccg gaatatcttc ataagtgaag
     1561 gtcatacctt gatcagcttc gttccagttg attgctttca ttttgattaa atcaacaaca
     1621 cctttgaagc tgtcttctgc accgattgga agttgtaatg caactgagtt accacctaaa
     1681 cgcgttttga tttgctcaac aacacgtaag aagtttgcac ctgtacggtc cattttgttt
     1741 acgaacgcga tacgtggcac tttatatttg ttagcttgac gccatacagt ttcagattgt
     1801 ggttgaacac caccaactgc acaataaacc atcaccgcac catcaagtac acgcattgaa
     1861 cgttctactt cgatagtaaa gtcaacgtgt cccggagtat caataacgtt gatacggtgt
     1921 tgtggatatt gttgtgacat accagaccag aatgctgttg ttgcagcaga tgtgatcgtg
     1981 ataccacgct cttgttcttg ttccatccag tccattgttg cagcaccatc gtgaacttca
     2041 ccgattttgt gacttacacc tgtatagaac aagatacgct ctgtagtagt tgttttaccc
     2101 gcatcgatgt gcgcactgat accgatgtta cggtaaagtg aaataggggt tgtacgagcc
     2161 atttttattt attaccttgt ttggggttta aattaaaatt cttaataagg gtaaggcttc
     2221 accgcaaact gagatgaagc ctttgataag tttgcttaaa cgattaccaa cggaagtgag
     2281 caaacgcttt gttagcttca gccatacggt gaacgtcttc acgtttcttc actgctgaac
     2341 ctttgttgtc tgctgcatct gataattcgt tagctaaacg taatgccatt gatttgtcac
     2401 cacgtttacg agcggcttct acgatccaac gcattgctaa agcattacga cgggttgggc
     2461 gaacttctac aggaacttgg taagtagaac cacccacacg gcgagattta acctcaacag
     2521 ttggacgtac gttttctaat gcgatttcga aagcttctaa cgcttcttta ccagaacgtt
     2581 gtgttaaagt ttctaatgca ccgtaaacga ttttttctgc agtagatttt ttaccatcta
     2641 ccattaacac gttaataaat tttgcaagta attctgaacc gaacttcgga tctggaagaa
     2701 ttttgcgtgg ttcaatacta cgacgacgtg gcattggaaa tttctccgta tattaaatct
     2761 tcaggatatc caaaactcat ctaatctgcc ttaaggcatc ttgactggag tttatggttt
     2821 aaatattaaa atttagacgt ttggccttac tcaacggaga accattaagc tttaggacgt
     2881 ttaacgccgt atttagaacg accttgttta cgatctttaa cgcctgcaca gtcaagtgca
     2941 ccacgtacag tatggtaacg aacacccggt aagtctttaa cacgaccacc acggattaat
     3001 acaacactgt gttcttgaag gttgtgacct tcaccaccga tgtatgaagt tacttcaaag
     3061 ccatttgtta aacgaatacg acatacttta cgtaatgctg agttcggttt tttaggtgta
     3121 gtagtgtata cacgagtaca cacgccacgt ttctgcgggc aagcctctaa tgcaggaacg
     3181 ttacttttta caaccttttt cacacgcggt ttgcgtacta gctggttgat agttgccatt
     3241 aaaaaagctc cagttaaaat tattaaaaat aaaattaaaa acatcctttc acacgaaagg
     3301 acggtgaatt ctaaattgaa cgtggataaa agtcaagcac agtaagagaa caacaatgat
     3361 catttgttaa tcaatggaag taatttttct agcacttgtg aagaggtaat attttccatt
     3421 ttatctgctt ttagataaaa ctgattttgc ccgtaagccc cgataagttt tggatcggtt
     3481 gccccatata aaatcacatt tggcttatct aacgcagccg ccaaatggct caagccggta
     3541 tccaccgaca ccactgccgc tgctcccgca agttgagtag caatatcggt taagctcatt
     3601 tttggcaata ccttagcatt attcgccaca ctcgctaaac gctccgcccg ttctttttcg
     3661 gcttggtttc cccaaggcaa tctgactgcc agattgtgct tatccaacgc ttgaatcaac
     3721 tccgcccaat aggcttcttg ccaatgttta tccgctcggg tagtggcgtg aatcagcata
     3781 atgtaagcgg tcgttttttg aggatttttt gcaaaatgtt gtgcaatgca ataatcgccc
     3841 cgttcggttg gcaaagcata acctaatgat tttgcacata attttctgat gcgttctaca
     3901 gcgtgttgtt ggtaggggat attaaacgtt tcatcgtaaa acagactact taacccttct
     3961 cgggcagagt ttttatcata gccaaatttt ttgcctttgg cttggcgagt tactaacacc
     4021 gcacttttaa tcagcccttg agcatcaatt accgcatcat actgctcact ttttagctgt
     4081 tttaaaaact gctgccattc tacccaagtc tgtctttggc aaaggttttt ccgccagcgg
     4141 cgaatagcca ccggaattac tttatttacc gctgaatgcc acgcaggaat ttcggtaaaa
     4201 ttctcctcca ccacccaatc tacttttaaa tcgggaatag cattttgcat atcggttatg
     4261 gctggaagcg tatgtaatac atctcccatt gatgaggttt taacgattaa aattttcatc
     4321 ttttataaac aaacaagcgg tctttttttg caaaaaattg gcaaattcaa ccgcttgtta
     4381 tccatttaaa ctaacagttc agctaatttg tccatcacca tttcaggctg aatatcaatt
     4441 aaactttggt gatagccctc tgccccttcg cctttgcgga ttttaatcaa accgccttca
     4501 attaagcgca tgatctcggc attttgggat aacggcggcg tatattgtgg gctggtcggt
     4561 ccatataagg cgaccagcgg tttatttagt gctgcggcaa tgtgcattaa gccggagtca
     4621 ttgctcacga ctgcattaca atctgcaatc aaatctaccg cttggtttaa atcggtttgc
     4681 ccggctaaat tcaggcaata gcgttgtaaa ttttcaggta atgccaagcg aatttgctca
     4741 ccggcggctt catctttttt cgagccaaat aaccgtaccg aatagccttg ctcaatcaac
     4801 atttttgcca atattgcata atgataatgc ggccaacgtt ttgccggtcc aaattcagcc
     4861 cctggacaaa agccgatggc aggacggttt tccgcataag ccaactgttt ttcaaatttc
     4921 gctttggtct gctcaatttg ttcgctatca gttgtgaggt atggataagc aatcggcaac
     4981 tgttgtgcgg ttggaacagt agccttttca aacgccaagg ctacatagcg ttgcaccatc
     5041 attgggtagt cgtttttatt actgcgaaga tcatttaaga aaaaatatct actttcccct
     5101 ttccaaccac gacgtctcgc aattttcgca aacagtggaa taaaggcaga ctttaatgaa
     5161 ttaggcaata caatcgccat atcatattga tttctcaggg attttccgag tcggtagcgt
     5221 tcacacaaac gaaatgaacc gtgtccaatc ggcattgaaa tcgcattacg cacctcaggc
     5281 atacgggcta aaagcggaca acaccaatcg ggtgccatta catcaatttg gcaattcgga
     5341 tactgtattt taagctgttg ataaagtgcg tgcgacatca tcatatcacc cacccaagac
     5401 ggtcctataa ctaaaatatt catttgcaaa attctcttaa aaatttattc acaatccttg
     5461 taaggattgt tcacagcttc gctgccgttg gcaaagccaa cgttcaaaaa gcgatacttt
     5521 ttgtgtccgc ttgttactgc tttatgcaat ttgccgctaa cgttttttta gtgttgttat
     5581 acaaaaatgc tttaccacta cgggtttcat gccaagtcca acggatatta gtgtattttt
     5641 taccatctct cgttacagaa ggtgaaagct gatgcgttgt cccttgataa ctcactgtga
     5701 ctatttcctt ttttaacgct ttttttgttc gtccctggcg ttcaacttca accttcttat
     5761 cattctcaca acgatataaa ctcactttag gtgcttgtgc tatcagttta gtcactttct
     5821 ttactgactc tttttccttt tgtacttgat gaatttgctc aacaggggta accactgtac
     5881 tacaagcaac taacatcgct gagcttagcg ttaagtagat aagtttgtaa gaggggcaaa
     5941 ataaattcat cctttatctc ctttttaaat aaaagagcaa cgtttgtagc gttgctcttt
     6001 ttcttcattt tatttattta aaagtatagc taccgtttgc ttgacgggta aatttgccgc
     6061 ctgagccaat gctcatttca accacacggt tatttttatc taaacgaacg gttactcgct
     6121 cgcctacttt taaattactc accacgttat tgactttgct catcgcatta acgtctgaga
     6181 tatttaaatt gttatcacgg aacacttgca ttagtgatac accttttggc acagtcattg
     6241 ttttggttgc taccgcattt gccgactcgg cagccggtgc tgcttttacc gcttcagttt
     6301 ttgtcggagc tttctcaact ggtttagctg ctgtttttgc cgtttctttt gctgccagtg
     6361 tagcacgttc ggttttcact tcagttttag caggggcttt aggctgttgt tttaccgttg
     6421 tttcaggttg gaacaccacg ctattttccg ttttaggctt aatcgccggt tgtgcaacaa
     6481 ccggtttatt ttccaccggt ttcaccgcaa caggttgctc aactactgct tgtgattgtg
     6541 tttgcgactg gctatcttgt gacggtttag ccattgtttg ctcggtttgg gctggagcaa
     6601 ctgttacaga ctcttgttga gcggcttgag cttgttcttg agcttttgca gcttcagcgg
     6661 cttcttgttc tgcacgagct tgttcttccg cacgtttcgc ttcagcttca tcaaccggtc
     6721 ggaattcaat cggcaagcta ccaccttgct gagattgtag ctcttcgaca gtttccggcg
     6781 tacttggttt taacaagaaa aacagtacca ataatgctaa cgccaacaat gccactaata
     6841 ataaacggcg ggttttaggt ggcattttag ctttaatctc tttggtttta gtggctgcag
     6901 ctgccgcagt ggctgctgcg gcagctgttg ccgcatttgg aataatacgc tcagtagtga
     6961 aactctcggg tgcaggttta accacttctt tttccacatt ttcttgtgct tcaaccgcgt
     7021 taaaagatgc ggttgataca ctttcttcaa ccgaatgaat ggtattatcc ggctctactg
     7081 cttctgtagc ttctgaactt ggagtaaatt ggaattcttt tgcctgctta gccgaaaaga
     7141 catcagccgt atgatcttct actttagcat caggtctttt cataattggg gtaaacgtat
     7201 gccccggtgc tttggttgaa cgcaacgaaa cataaggggc ttgagctgta tctgttttgg
     7261 ttgcttcagc gtcttgagag gtaaccgaag attcaccaaa aaccggttct ttgcgaaaat
     7321 tattttgtgt catattaatt ccaaataata gcgttgttaa tgagtataaa aatggcgata
     7381 ttctaaagca attcagccgc tatttgaatt tatttaacta aatttacgca actagcggtc
     7441 atttttgcct aattttttgc aaatctattt actttcattg aatttcagac caaatgcacg
     7501 tctagaagtt tagccatttt ctattttttc gatctgtgtc gaaaaattta tttttgccgg
     7561 ttgagttcta ttttattccc tcttaatctt cctttcgacc tttcaacaac taataaggaa
     7621 atcctatgaa gaaaatcaaa caacttggtc taacattctt gtttggctta aatgcaatgg
     7681 cttttgccca aacaccgcca actacaatgc ctgttgaaca aaatgcagtc actcaacccg
     7741 tacagcaaac acaaatcacc aacgctaatg cggtaaatat caatactgct agtgctgctg
     7801 aattgcaaga caagctagtg ggaattgggc agaaaaaagc tcaagcgatt gtcgattacc
     7861 gtactaaaaa cggcaatttt aaaaacattg agcaacttgc tgaagtctcc ggaattgggc
     7921 aagcaacggt agaaaaaaat cgagccaata ttattttaga ataagacaac caaattgttg
     7981 aagtggaatt cctacaaagc ccctatctgt ttttttcttt gtagccactt caacacctta
     8041 aagatttttt atctattttg cattgtattt gctatacttc gcccccaatt aaacgcaaat
     8101 tctcgttttt aaataaaagg aaaacgcaat gcaattaaaa aaatatccag ttccaaccta
     8161 tttccaacgt gtgaaaatcg ctctgcacta tttactgcca caactggcaa ttacccgtgc
     8221 ggcaggttgg ttagcagagc aaaaatgggg cgtagttact caccttatca tcaaactgtt
     8281 tgcaaaacag tataaagtca atttatctga agctaaaaaa accgagccgt cagattatgc
     8341 tacttttaat gaatttttta tccgtgaatt aaaagaaaac gcacgcccga ttaatcaaga
     8401 tccaaataca ctttgcttac cggcagaagg taaagtcagc gaatcaggcg aaatttcgga
     8461 taaccgttta ttacaagcaa aagggcatca ttttacgcta gaaaccttat tggcaaatga
     8521 tgaagaaatg gcggcaaaat ttaaagacgg cacatttatt accacctact tatcgccaac
     8581 tgattaccat cgtgtacata tgccgtgcga tggaacattg cgtaaaatga tttatgtgcc
     8641 gggcgaattg ttctccgtca atccgttttt agccgaacac gttccaaact tattcgctcg
     8701 caatgagcgt gtgatttgtg aatttgacac cgcattcgga ccaatgctac agattttggt
     8761 cggtgcaacg gttaccgcaa gtatgagtac ggtttgggct ggcgtaatta atccgcctcg
     8821 cgctgatgaa gtggtagttt ggaattacga aaccgaaggc gaaaaagcca tcaaactcca
     8881 aaaaggcgaa gaaatgggag ctttccgttt aggctcaacc gtaattaacc tcttcccgaa
     8941 aggtaaagta catctcaacc ctgcacttat tgcaggaatg caaacccgaa tgggggaaga
     9001 gttgggtaga attatcaatt aaatcctgag ttaattttac ctaaactgtt tgctataatc
     9061 ttccgataag aaactcttat cggaaggaaa aatatgtaca cacttcaaga attaaagcag
     9121 atgaacgagc aagaaattcg ctctcgatta atctctcctg caattagaaa tgcaggatgg
     9181 tcggatagac aaattggcga agagtacaca tttaaaacca ataaacgatt tacagatggg
     9241 caagtagttg ttgatccaaa aacgacgcaa actaaacgca tcgaagcaaa acgggttgat
     9301 tatctgctct atacttccgc aaatcaaaaa attgccgtgg tcgaggccaa agataaccat
     9361 cactcttccc gacacggctt acagcaagca atgacatacg ctcgattgct tgatgtccct
     9421 tttgcatata gctcaaatgg tgatgagttt gtggaacacg attttatcac tggcgtacaa
     9481 cgaacccttc cgatgtcagc atttcctact cccgacgaac tccaccaacg ttgggcgaaa
     9541 caatatacca ccgaagagct gaaaattatt caacagcctt attacaccag tagcactgac
     9601 acctttaaag agcctcgtta ctaccaagaa attgcgatta accgtactgt tgaagctgta
     9661 gcaaaaggtc agcagaggat tttattggta atggcaacag gcacgggtaa aacctatacg
     9721 gctttccaaa ttatccatcg tttaaaaacc agtggtgcga aaaagaaaat tttatttctt
     9781 gccgatcgca atgtactagt cgatcagcca atgatgcaag acttcaaacc gtttaaagac
     9841 aaaatggtga aagtcagcaa taaaaagctc gacagtgctt atgaagtcta tcttgcactt
     9901 tatcaacagc ttgacggtga aaccgataac gaactgttta agcagttcca accctctttc
     9961 tttgatctta ttgtgatcga tgaatgtcat cgtggttcag caaaagaaga ttctgactgg
    10021 cgtgctattt tgaagtattt caaggacgcc acccatatcg gtatgactgc aacccccaaa
    10081 gaaaccgatg atgccagcaa cattacctat tttggcgagc cgatttatac ctatagcctc
    10141 aaacaaggta ttgaagacgg ctttcttgcc ccttatcgag tgattcgtgt tgatctggac
    10201 tgtgatttaa aaggctggcg accaaccaaa ggtttaaaag atcatcaagg taatgagatt
    10261 gaagatcgcc tctatacagt caaagatttc gattacaaca ttgtgctaaa tgatcgcacc
    10321 cgcttggttg cggagagaat ttgcgaattt ttacgcaaaa ccgaccgctt gcagaaaaca
    10381 atagtcttct gtgccaacat caaccatgca gagcgaatgg tggcagagct ttcacgcctc
    10441 aatgccgata tgttgaaaca caattccaaa tatgtctgcc aaattacagg cgaccaatca
    10501 ggcaaaggcg ttgatttaga agaattttgt gatgtagcaa atgaagggaa agattaccct
    10561 gtgattgccg taacctctaa attgatgaca acaggtgtag atagcaaaac ctgtaagctg
    10621 gttgtacttg atgcaaacat tcaatcaatg acggaattta aacaaattat cggacgaggc
    10681 actcgtcttc gccctgattt cggcaaagaa tttttcacca ttttagattt tcgaggcgtt
    10741 actcgcctgt ttgctgatcc ggcatttgat ggtgagccgg tggtgatttt agatgtaccg
    10801 gaaaataagc cacttgtcga acaaccgctt cctaaaccca gtgataagga catcattaaa
    10861 gaccccgagc taaagccata taaaatcccg gtagttgtcg atttcggcac actcaaatta
    10921 cgcattttag acgagaaagt ccaatatctg aatgagcagg gcgagctggt ttctgaagat
    10981 attgatctgt tctcaaaacg ggaaatgcaa aaagagttcg gcagttacga acagttccaa
    11041 cagcaatgga caacaagcgg tgaaattttc tcgaaatttt acaccgatcc aaaatggctg
    11101 gcagcccttc gccaaacacg ccaattcagc cacgatgttg aagactttga tgtattaagc
    11161 cacatctcct ttggcaaaaa accactgact aaaaccgaac gagcagaaaa agtgaaacaa
    11221 agtggctatg tggagcagta ttcgcccgaa aaccagcaag ttttagggtt gctgcttaat
    11281 gaatatgtca aatccggcaa tcaagatctg actaaaaccg aaattttaaa gacaccgcaa
    11341 ttcagccgtt ttggaggtct agtgtctatt attaaaaatt tcggcggtaa agaactctac
    11401 cagaaagcgg taaaaggctt agaaaagatg atttattcgg aataacaagc ggttatttta
    11461 caacaaaatt ttacagataa acaaggatca caatgagcct caacaacata gtaaaacgcc
    11521 tgcaagatgt aatgcgtaac gatgcaggga ttaacggtga tgcccaacgc attgaacaga
    11581 tcgtctggat cttattcttg aaaatttacg atgctaaaga acaagagtgg gaactggaaa
    11641 acgatgaata tcactcaatt ttgccgaatt ttttacgctg gcaaaattgg gcggaagata
    11701 aaaaagacgg caaagcgatg actggtgatg aattactctc tttcgtcaat aacgaacttt
    11761 tcccgacttt aaaaaatctg cctatttcag ccgatacgcc gatgaaccaa cggattatcc
    11821 gtgctgcttt tgaagataat aacaactata tgaaaaatgg ggtgttatta cgtcaggtta
    11881 tcaatatcat tgatgaaatt gattttgccc attaccaaga acgacacgcc tttggcgata
    11941 tttatgaaaa tatcctcaaa agtttacaaa gtgcaggtaa tgcaggggaa ttttataccc
    12001 ctcgcgctgt aaccgatttt atggtacaag caatcaaacc gaaacttggc gaacgcattg
    12061 ccgattttgc ctgtgggacg ggtggttttt taacttcggc gttaaaggta ttggaaagtc
    12121 aaattcaaac cctttctgac cgcactttat tcaataattc tgtgtatggc attgagaaaa
    12181 aagcccttcc tcacctgctt tgtattacca atttattgtt gcacgatatt gataatccga
    12241 atgtacatca cgataatgct ctagaaaaat cggtcaaaga ttacaccgaa aatgataaat
    12301 ttgatgtgat tttgatgaat ccaccttacg gaggttcaga aattgagcaa attaaaaaga
    12361 atttccctac ggctttacag agcagtgaaa ccgctgatct gtttatgtcg gtaattatgt
    12421 accgcctaaa acagaatgga cgggctgcgg tggtgctgcc tgacggattt ttgttcggca
    12481 cagataatgc caaagttgcg attaagaaaa agctattaga agagtttaat ctgcatacga
    12541 ttatccgctt accgcatagt gtctttgccc cttatacctc gattacgact aatattctgt
    12601 tttttgataa aaccgagcca acccgtgaaa cttgggttta tcgccttgat atgccacagg
    12661 gttataaaaa tttctccaaa actaaaccga tgaaattgga acatttcaac gaagttgttg
    12721 aatggtgggg aaatcgccaa gtgattgaag tcgaagggtt tgacaaagcg aaaagtttta
    12781 cattaaagga aatgtccgac cgtcaataca acctcgattt atgtggcttt cctcacgaag
    12841 aagaggaaat ccttccgcct gatgaactaa ttgcagatta tcagcgaaaa cgcaccgctt
    12901 acaatgctga tattgataag attctagcag aaattaccga tattttaggc attaaactgt
    12961 aataggagca taaaatgctg acttgggaac atatcgaaaa aatgattgca catcagcaat
    13021 ttatccgaga aaaggcagaa attgaatata aagaggcaaa aaatagtgtg ccaaaagatc
    13081 tttggcatac ttattcggct tttgcaaaca gcaatggtgg ctttatttta ttaggaataa
    13141 aggaaaatga acaaggacat tttcctgaac tgctaatttc tggtgttaat gatcctgata
    13201 aagtcattga tgaattattt tcccaatctc gtagtgaaaa agtcagtaaa tccttattaa
    13261 aggataatga tgtgaaaatc tttcaagttg aaaataaaag cgtgattgca atttatgtga
    13321 aagcagctga tattttagac cgtcctatcc atttaaatgg tgattatcga ctttcttatg
    13381 ttcgtttaaa ttcaggcgat cataaactca cctcctctga actgcgttct ttcatttcaa
    13441 gctatagcca aaaagatgct gatgggagaa ttgtgcctta tgcttctatt gatgatatca
    13501 gtttaagcac attggataaa tacagaaatc tgctgaaagc ccataacagc acatcaaaat
    13561 tacttacgtt atccgatgag aaattgctgc aagaaattgg ggcttataaa aaagatttag
    13621 cacagaatag cgagggcatt accaatgcag gcttattgat gtttggtaag cactatgcca
    13681 ttacaagcat atttccccac ttcttttttg aatattacga agaagctggt gatgtaaaac
    13741 gctatacatt acggattacc gattttgatc ttgaagaagg caatctcttt gaattttact
    13801 tgaaaattgt gccacttatt caagagcttg gtaaagataa acatttcaaa ttagataatt
    13861 taacccgtac tgaggaaaat gaaattactg acgggctacg ggaagctcta atcaatgcaa
    13921 ttactcacgc agattacttt aacaatgttc gccatctaaa agtcattaaa accaaagata
    13981 acaaactgat ttttgaaaat gcaggagtaa tgttggttga tattcccacg gctattgcag
    14041 ggcaaagatc ggaatgccgt aattctatta ttcataactt attccaccgt ttagggctat
    14101 gtgagcgaca aggacaaggc gttcgggata tttattcata ttggcaaagc cgaaatttca
    14161 attcaccgct tttagaaagt gaaattgatt acactaaact caccttaact tttcaatcag
    14221 gtatgattgc agattttgag gaaaaattta ccgcttgctt tggcgaaaat ttctctgagt
    14281 tacagcctat tcaaaaagat tgcttactct ttatcgcctt caatcaaggt aaagcaatgt
    14341 ttgcagaatt agcacaaaaa ctgccccaat atccacgcag aaatattact ttggcactag
    14401 cacgactgaa agaaaaaggc ttactaacca gttcggggag tccgagaaaa caactctata
    14461 ccctagtgaa tttagaatgg ataatcacag agaaatcaca agatgattat tttaatccac
    14521 ctttcgacca acttgataaa gctagtgaga gtaagggcgc ccccccgaag aaaagcaaag
    14581 tgaagcaagt caaacaatta ccccagaaca aagcaaacaa attacccccg aagtaaatcg
    14641 cttactttca gtgttgcttg agccgatgaa gagcaaagaa attatgaaaa aactcaggtt
    14701 aaaagatgat aagcatttcc gtatgcagta tttaaaacct gcactaacgc ttggtgtgat
    14761 cgaaatgcaa ttcccagaaa acccaaaaca ccgtaatcaa agatacttta agaaataaca
    14821 accgattatt gagaataaaa aatgaaagcc caacagctaa aaaacgcaat cctacaacta
    14881 gcaatccaag gcaagctcgt tccacaagat ccaaatgatg aaccggcaag cgaactcctt
    14941 tgcaaaatcc aagcagaaaa agaccgcttg attgcagaag gaaagatcaa aaaaaacaaa
    15001 aaaactgccg ataaagcccc ttataccatt gaaccacctt ttgagattcc tgagagttgg
    15061 gtgtgggtga ggttggggga tatatgccta aaaattacag atggaactca tcattctccc
    15121 ccaaatatcg ataagagtga ttttctttat attacagcta aaaatataaa gaaagatgga
    15181 ttagatttat caaaaattag ctatgtaaca aaagaaattc ataatgaaat tttcagccgt
    15241 tgtaatcctg aaaagggaga tattctatat attaaagatg gagccacaac tggagtaagt
    15301 attataaata ctcttaatga gccatttagt atgttatcca gtgttgcctt aattaagacc
    15361 tcacaagaaa tagataatga gtacttaaat tatgtaatga atagtcatta tttttataac
    15421 atttctattg gaagtatgtc tggaacaggt attccaagaa taacattaac taaattagaa
    15481 tcttatttag tcccagttcc gccactccta gaacaacagc gcattgtgca aaaaatcgag
    15541 gaattattac cgcttgttga acgctacgag caaaccgaac agcaactcac taaactcaac
    15601 aatacattcc ccgaacagct gaaaaaatcg gtgttacacg cagctattca gggtaaatta
    15661 accgagcaag atccgaatga tgaactggca tcttgcctga ttgagcggat aaaagcggag
    15721 aaaaatcgtc ttattgccga gaaaaaactc aaaaaaccaa aaagtgtttc agagattgta
    15781 atgcgtgata atttacctta tgagatcaaa gcaggtcagg aacggtgtat cgccgatgaa
    15841 gtgccatttg agattcctca aaattggact tgggtgaggt tgggggaagt aggtgattgg
    15901 agttcaggtg caactccaaa ccgacacaat cctgaatact ataatggtac gatcccgtgg
    15961 ctaaaaacag gagacttaaa caacggaata atttcatcta tacctgaatt tattacagat
    16021 aaagcattgc aggaatgttc tgtaagatta aatcctgttg gttcagtttt gatagcaatg
    16081 tatggagcga caattggtaa gcttgggata ttaaaaatag cagcaacaac aaatcaggct
    16141 tgctgtgcgt gtattccttt taatggaatt tataataaat tcttatttta ttatttgatg
    16201 tcgcagaaag cagaatttca gaaaaaaagt gagggaagtg ggcaaccaaa tatatcaaag
    16261 gaaaaaatta tcaattattt atttccttta ccaccaatcc acgaacaaca tcgcattgtt
    16321 caaaaaattg aacaattatt tgcagagatt gaaaaattgt aggagtcatc atcgctttct
    16381 tgggcgtgtt ggatacgccc tacagtcact aaattattga tcttttatca tatcggtttt
    16441 caaccaacct aaaaggaaac ctatatgatc catctatttg aaaagcacct taatcaatct
    16501 aatcttgctg aaaataccca atcagcttat ctttttgcgg taaaacaata tcacaaaaaa
    16561 tttgatcagt tatctaaaat
//
LOCUS       DANLRK010000040        16518 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.040, whole genome shotgun sequence.
ACCESSION   DANLRK010000040 DANLRK010000000
VERSION     DANLRK010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 16518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16518
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.040"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(107..622)
                     /locus_tag="PXA33_000924"
     CDS             complement(107..622)
                     /locus_tag="PXA33_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDL5120270.1"
                     /translation="MAGINKVIIVGNLGNDPEMRTMPNGEAVANISVATSESWTDKNT
                     GARREVTEWHRIVFYRRQAEVCGQYLRKGSQVYVEGRLKTRKWQDQNGQDRYTTEIQG
                     DVLQMLGSRQGNDMGGQQGGWGNSAPAGNSYNQGNSNYGYNQTQSAAPQSKPAPQPEP
                     AMNNFDDDIPF"
     gene            777..3605
                     /gene="uvrA"
                     /locus_tag="PXA33_000925"
     CDS             777..3605
                     /gene="uvrA"
                     /locus_tag="PXA33_000925"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005612043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDL5120271.1"
                     /translation="MQHIDIRGARTHNLKNINLTLPRDKFIVITGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTVT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTISQMVDRVLEEPEGKRLMLLAPVVKERKG
                     EHVKLLENLTASGYLRARIDGEICDLSDPPALELQKKHTIEVVVDRFKVRADLATRLA
                     ESFETALDLSGSTAIIADMDDPNAEEIVFSSSFACPHCGYSLHELEPRLFSFNNPAGA
                     CPTCDGLGVEQFFDAKKVVQNEEVSLAAGAIKGWDRRSFYYFGLLKAVAEHYGFDLET
                     PFGQLPKKYQNIILNGTKEEIEFVYVNDRGDKVKRKHAFEGVLNNMARRYKETESNSV
                     REELAKYINNRPCMDCEGSRLRREARYVFLDKTNLPMVSEKSIGEALTFFEELKLAGQ
                     KEKIAEKILKEIRERLQFLVNVGLNYLSLSRSAETLSGGEAQRIRLASQIGAGLVGVM
                     YVLDEPSIGLHQRDNERLLNTLVHLRNLGNTVIVVEHDEDAIRAADHIVDIGPGAGVH
                     GGQVIAQGTAAEIMQIEESITGQFLSGKQKIEIPAQRVPYEPTKLLTLSGASGNNLKK
                     VKLEIPVGLFTCITGVSGSGKSTLVNDTLFPLAQNALNRAENSDVAPYESIDGLSHFD
                     KVIDIDQSPIGRTPRSNPATYTGLFTPIREIFAGTQEARARGYNVGRFSFNVRGGRCE
                     ACQGDGVIKVEMHFLPDVYVPCDHCKGKRYNRETLEIRYKGKTIHQVLEMTVEEAREF
                     FAPMPMIARKLQTLMDVGLSYIRLGQSSTTLSGGEAQRVKLATELSKRDTGKTLYILD
                     EPTTGLHFADIKQLLGVLHRLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGEI
                     IATGTPEEVAKDKRSHTARFLKEILAKG"
     gene            complement(3697..5430)
                     /gene="dauA"
                     /locus_tag="PXA33_000926"
     CDS             complement(3697..5430)
                     /gene="dauA"
                     /locus_tag="PXA33_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HDL5120272.1"
                     /translation="MLNKWFLTKNAFLAVKPFSALKDALNAGYGKQHLIRDIISGLTV
                     GVIAIPLSMALAIASGVPPQHGLYTAIVAGIVIALTGGSRFNISGPTAAFVVILYPVT
                     QQFGLSGLLMATMLSGGILVLMALLRLGRLIEYIPLPVTLGFTCGIGIVIATLQIKDF
                     LGLSIEKMPEHYLGKLEAIFSALPTINWADTLVGVVTLLVLTRWHKLRLPIPGHLPAV
                     FIGTLLSLLLIHFGQNVATIGSAFHYTLSDGTTGYGIPSVLPEFVLPWNLPNAEGNRI
                     DWNLTTIQALLPAAFSMAMLGAIESLLCAVVCDNMTDTKHHSNNELLAQGLGNIASPF
                     LGGITATAAIARSAANVRSGAVSPISSVVHALLVLFALLFLAKALSYLPLSSMAALLL
                     VVAWNMADIPQIIQLTRRSGKNEIAVLLTCITLTVLFDMVIAITVGVLLASLLFIRTI
                     AEMTKSFEMAHPTDLNDVLVYRISGPLFFAAADNLFADLHNKTVHTDHQIKHIVLQCD
                     AVTVLDAGGIHALTRFVQHMLPHQQLHLCNVQFQPMRMLVRSQKTVNEIPQIQFSSDL
                     ESAFDKIRQAN"
     gene            5672..6604
                     /locus_tag="PXA33_000927"
     CDS             5672..6604
                     /locus_tag="PXA33_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL5120273.1"
                     /translation="MDSLIHFLSAFLTNITWIGALELGLIYSLVALGVLISYKILDFP
                     DLTADGSFPLGGAVCVISILNQVDPWLATLYGTLAGAVAGMVTASLHIGFKIEKLLAS
                     ILMMIALYSINLRIMGMPNIAILGETTIYDVMPIESDLQLALVRLVMALLVVIIVKGL
                     FDLFFATEIGLAVRATGTNSRMAKAQGIAINKMTILGMAISNALIALGGALYVQSNGG
                     VDISIGVGTIVIGLAAVIIGEAIFSVKRIIWLTFAVIIGSILYRFFIALALNNETLNG
                     IGFGPQDLNLITALLVVIVLAVPRIKHKLLGKRG"
     gene            6606..7403
                     /locus_tag="PXA33_000928"
     CDS             6606..7403
                     /locus_tag="PXA33_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015432965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL5120274.1"
                     /translation="MIELNNLFITFNKGTAIENPVLRGLSLKVEQGEFVSVIGSNGAG
                     KSTLLNAISGDCAVDSGEILIQGNKVNQSTTWQRANQVARVFQDPMAGTCESLTIEEN
                     MALAYQRGRKRGLGFAIKRQMRELFKDKLALLGLGLENRLTDQMGRLSGGQRQAVSLL
                     MASLQPSNILLLDEHTAALDPKTTDFVLELTNKIVREQKLTTLMVTHSMRQALDYGDR
                     TVMLHQGQVAFDVSGEQRQKMDVPDLLQLFQQSRHEQLSDDGLLLGN"
     gene            7490..7711
                     /locus_tag="PXA33_000929"
     CDS             7490..7711
                     /locus_tag="PXA33_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5120275.1"
                     /translation="MLSIFKTPVEKETLDDWAKISVDVAKVAILAIPVILFGKEPTLI
                     KLTNLALLVLSIYVFLTLGRKLRQLKEVL"
     gene            7894..9819
                     /locus_tag="PXA33_000930"
     CDS             7894..9819
                     /locus_tag="PXA33_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HDL5120276.1"
                     /translation="MTKYDDKIQHAFSENGLLSQNISGFRPRQAQLEMAQVVAKAVKF
                     ATPVVVEAGTGTGKTFAYLVPALLSGKKTILSTGSKNLQDQLFNRDLPTIQKALKYKG
                     KIALLKGRANYLCLERLDQVTAMGVLGDKSVLTDLKKVSKWQNATKTGDLSECITIAE
                     DSPILPQLVSTTESCLGSDCPHFKDCYVVQARRKAMEADVVVVNHHLFCADMAVKKTG
                     FGELIPEAELVIFDEAHQLPDIASQYFGQSLSSRQLFDICKDTNIVYRTELKDLSQLG
                     KAADHLTKVVQDFRLLMGGEGQIRGNLRELFRDKKVVDGLVKVAENIDFLSEVAKKSL
                     GRSETLDKIFERLAEVKVQLKKLMDTSVTGYCYWYEANGRSFGLHITPLMVADKFGEQ
                     LKNQQIGWVFTSATLEVGGKFDHFCQRLGIENAEQLVLQSPFDYQHQSLLCVPRYLPD
                     TNKSNTLTALGQLLKPVIEANKGRCFLLCTSYYMMRGLADFLREHSSLNVLLQGETSK
                     SRLLEKFVKEDHSVLVATQSFWEGIDVRGDDLSLVIIDKLPFTSPDEPLLKARMEDCK
                     IQGGDPFNDIQIPEAVITLKQGVGRLIRDVTDRGVVIICDSRLVMRNYGQTFLKSLPP
                     SRRTRDLGNVIQFLKQK"
     gene            9893..10258
                     /locus_tag="PXA33_000931"
     CDS             9893..10258
                     /locus_tag="PXA33_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HDL5120277.1"
                     /translation="MLSTLLRMVASLALLFVMLYLGKLIVYLFPIGIPDSILGMLILF
                     GCLVIGIVKVEWVIPSAKPLTRYITLFFLPICVELIEHLELLRQNLNTFVLATALSTL
                     VSVVAIGVFAQWIFHYKRG"
     gene            10260..10946
                     /locus_tag="PXA33_000932"
     CDS             10260..10946
                     /locus_tag="PXA33_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HDL5120278.1"
                     /translation="MSYLYTVLTVIMFIAGLKISQKLKSSFLNPFLIALALLIGVLLI
                     FNIPYQDYYQGNSPLNNLLGVSVVALAYPFYEQLPHIRKKWRQIVVILLFSTIITMIT
                     GLGFSLLFGASQEILAAILPKSVSMPMAIIITNEINGNVAIAAVGVMIAGLVGSILGF
                     TLLRLAKVRNVMVIGLSMGAASHALGTARCLEHSMKAGSYSSIALVACGVLSSIFAPL
                     IFNVIVTLCY"
     gene            11030..12793
                     /gene="msbA"
                     /locus_tag="PXA33_000933"
     CDS             11030..12793
                     /gene="msbA"
                     /locus_tag="PXA33_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HDL5120279.1"
                     /translation="MQEQITQEKDLSTLKTFKRLWPTISPFKAGLITGAVALVLNALV
                     DSSLIYLLKPLLDEGFGQANNDFLKLMAVLVVVFILVRGITNYIASYCLAWVSGKVVM
                     TLRRHIFQHLMYMPVSYFDNNPTGRLLSRVTYDTEMVASSASHSLVTIVREGAYLLSL
                     FVVMIYTSWQLSIVLFVMAPIIGVLISFVSKYFRQLSRNIQSSMGELTVTTEQMLKGH
                     KVVLSFGGQEIEKARFDRVSNDMRRKGMKVVSADGISDGVVQLIASFALSAVLYVATF
                     PEVMSENLTAGSFTVVFSSMMAMLRPLKSLTNVNSQFQRGMAACQTLFEFLDLKTEKD
                     HGLISVAKAEGNVEFKKVSFSYEGKEEKALDNISFTIPKGKTVALVGRSGSGKSTIAN
                     LLTRFYDVTDGQILLDGINIQDYRLKNLREQCSVVSQQVHLFNDTIANNIAYAAIDKY
                     SREEIIAAAKAAHAMEFIEKLENGLDTVIGENGASLSGGQRQRLAIARALLRNSPVLV
                     LDEATSALDTESERAIQSALEVLQKDRTVLVIAHRLSTIEKADEILVVDHGNIIERGS
                     HTELLAQGGAYKQLHSLQFGA"
     gene            12882..13514
                     /gene="lolB"
                     /locus_tag="PXA33_000934"
     CDS             12882..13514
                     /gene="lolB"
                     /locus_tag="PXA33_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HDL5120280.1"
                     /translation="MKKLTKLLSLSALLVLTACNSVLNASTEVQQPTVQIPHDDPQWQ
                     QHLAKLSQIQGYTATGQFGYISPEERFSSHFNWRYTNPIQFGLELSSNLSTKSLKLER
                     NKRGLTVSDNEGNSRSDRDVDALMQEIIGVSFPIDQFAYWVKGLPEKDGNYIVNEKRQ
                     LSQFSYPLNGTLWKASYVEYHEDRVPNLPKLIVLENGTQTLKIRIEKWAY"
     gene            13502..14356
                     /gene="ispE"
                     /locus_tag="PXA33_000935"
     CDS             13502..14356
                     /gene="ispE"
                     /locus_tag="PXA33_000935"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HDL5120281.1"
                     /translation="MGVLMKKVTLPSPAKLNLFLYITNKRADGYHELQTLFQFLDFGD
                     EIEIEITESSEVELINPVEGVATEENLIYRAAKLLQKTTACRQGCRIGIKKNLPMGGG
                     VGGGSSNAATVLVGLNHLWNTELSLEKLAELGLSLGADVPIFVRGMAAFAEGVGEVLT
                     PCDPQEKWYVVLKPEVSISTAAVFTDPNLPRNTPKRPLAELLSLPWENDCEKVVREKY
                     SAVEDLIQELLQYAQFRLTGTGACIFAEFETKAEAEQVFAHKPEAVFGFVAKGQNISP
                     LHQMLFQI"
     gene            14382..15332
                     /locus_tag="PXA33_000936"
     CDS             14382..15332
                     /locus_tag="PXA33_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HDL5120282.1"
                     /translation="MTDIKLFAGNATPELANRIAKHLYTSLGNATVGRFSDGEIQVQI
                     NENVRGGDIFIVQSTCAPTNDNLMELIVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVIADFLSSVGVDRVLTCDLHAEQIQGFFDVPVDNVFGSPVLIDDILKKS
                     DLVNPIVVSPDIGGVVRARAVAKLLNDTDMAIIDKRRPKANVSQVMHIIGDVADRDCI
                     LVDDMIDTGGTLVKAAEALKERGARRVFAYATHAVFSGTAAQNLANPALDEIVVTDTI
                     PLSNEIKALNKVRVLTLSGMLAEAIRRISNEESISAMFDA"
     gene            15410..16420
                     /gene="pyrD"
                     /locus_tag="PXA33_000937"
     CDS             15410..16420
                     /gene="pyrD"
                     /locus_tag="PXA33_000937"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HDL5120283.1"
                     /translation="MYSLIRKLLFALDTEKAHQFSIQALALAGKSPFSILPTPDNPTQ
                     VMGLTFKNPIGLAAGADKNGEAIDGFGKLGFGFIEVGTITPLAQDGNPKPRQFRIVDA
                     EGIINRNGFNNKGVDVLVENVKNATYSGILGINIGKNAVTPIEHSFDDYQICLRKVYQ
                     YADYVAVNISSPNTKNLRSLQYGEALDDLLRNLKNEQAQLSQKMGKYRPLVLKIAPDL
                     TDEEIVSVADSLIRYQIDGVIAGNTTLSRESIAGLPYSEQLGGLSGKPLHSLSTRLIS
                     KLSAELGERIPIIGCGGVHSLESGQEKVNAGASLLQVYSAMIYQGPSLIQTLAKHLQL
                     NS"
BASE COUNT         4753 a         3124 c         3823 g         4818 t
ORIGIN      
        1 gttccctatt attttgagta aggtaggggc gctttatatg agtagctttt tatttcatcg
       61 aaatgcggtg tatggaaccc atgagctact taagtcaata ctttgattag aacggaatat
      121 catcatcaaa attgttcatt gccggctcag gctgtggtgc cggtttagat tgcggtgcag
      181 cactttgggt ttggttgtaa ccataattgc tgttgccttg attgtagcta ttacccgcag
      241 gagcagaatt tccccaaccg ccttgctgac cgcccatatc attaccttga cggctgccga
      301 gcatttgtag cacatcgcct tgaatttcgg tggtgtaacg atcttgcccg ttttggtctt
      361 gccatttacg ggtttttaaa cgtccttcta cgtaaacttg agagccttta cgtaaatatt
      421 gcccgcatac ttctgcttga cggcggtaga atacaatacg atgccactcg gttacttcac
      481 ggcgtgcacc ggtgttttta tccgtccagc tttctgaggt tgccacacta atatttgcaa
      541 ccgcttcgcc atttggcatt gtacgcattt cagggtcgtt acctaaatta ccgacaataa
      601 ttactttatt gataccagcc ataaaatcct ctgtttgata aattaaacta aaatttgcta
      661 agtattctac ctgaaaatcc aaaagatgaa caccaaaaaa ctgatatttt gtacagtttt
      721 attaaaatat gcgataatga tcgaaaattg agaaaatagc actggacatt aaaaatatgc
      781 aacatatcga tattcgtgga gcaagaacac acaacttaaa aaacatcaat ttaaccttgc
      841 cgagagataa attcattgta attaccgggc tttccggctc gggcaaatcg tctctggcgt
      901 ttgatacgct ctatgcggaa ggacaacgcc gttatgtaga atcactgtct gcatacgccc
      961 gccagtttct ctccttaatg gaaaagcctg atgtggatca tattgaaggg ctttcccctg
     1021 cgatttcgat tgagcaaaaa tcaacctctc acaacccacg ttcgaccgta ggaacggtaa
     1081 cggaaattca cgactattta cgtttattgt ttgctcgagt gggcgagcct cgttgccctg
     1141 accacgatgt gccattggct gctcaaacta tttcgcagat ggtggatcga gtattggaag
     1201 aaccggaagg aaaacggttg atgctacttg cccctgtggt gaaagagcgt aaaggcgaac
     1261 acgtaaaatt attggaaaat ttgaccgcta gtggctattt gcgggctaga attgacggtg
     1321 aaatttgcga tttatccgac ccaccggcat tagaattaca gaaaaaacac accattgaag
     1381 tggtggtgga tcgctttaaa gtaagggctg atctagccac ccgtttggcg gaatcttttg
     1441 aaacagcgtt agatttatcc ggctctaccg caattattgc agatatggac gacccaaatg
     1501 ccgaagaaat tgtgttttcg tccagctttg cttgtccgca ttgtggttac tctttacacg
     1561 aattagaacc acgccttttc tcgtttaaca acccggcagg tgcttgcccg acttgtgatg
     1621 gtttgggcgt tgagcagttt tttgatgcca aaaaagtagt gcaaaatgaa gaggtttcat
     1681 tagccgccgg tgcgatcaaa ggctgggatc gccgcagttt ctactatttt ggcttattaa
     1741 aagccgttgc cgagcattat ggctttgatt tagaaacccc attcgggcag ctaccgaaaa
     1801 aatatcaaaa cattatttta aacggcacta aagaagagat tgagtttgtt tatgtcaatg
     1861 accgaggcga taaggtcaaa cgtaaacacg cttttgaagg ggtgctgaat aatatggctc
     1921 gccgctataa agaaaccgaa tcaaactcgg tgcgtgaaga gttggcgaag tatattaata
     1981 atcgtccttg tatggattgc gaaggctcac ggttacgccg tgaagcccgt tatgtattct
     2041 tagataaaac caatttgccg atggtttcgg aaaaaagtat cggcgaggca ttaacctttt
     2101 ttgaagagct gaaattagcc ggtcagaaag aaaaaattgc tgagaaaatc ctcaaagaaa
     2161 ttcgtgaacg gctacagttt ttagtgaatg tcggcttgaa ttatctttct ctttcccgct
     2221 cggcagaaac cctttcaggt ggcgaggctc agcggattcg ccttgccagc caaattggtg
     2281 cgggtttagt aggggtaatg tatgtgctgg atgagccttc catcggcttg caccaacgag
     2341 acaatgaacg cctactgaat actttggtgc atttacgcaa tttaggcaat accgtgattg
     2401 tggttgaaca cgatgaagat gcgatccgag ccgcagatca tatcgtggat ataggcccgg
     2461 gtgccggtgt acacggtggg caagttatcg cacaaggtac ggcagctgaa attatgcaaa
     2521 ttgaggaatc tattaccgga caattcctct caggcaagca aaaaattgag attccggctc
     2581 agcgtgtgcc ttatgagcca actaaattgc tcaccttatc gggagcaagt ggtaacaact
     2641 tgaaaaaagt gaaattagag attccggttg gtttatttac ttgtatcacg ggtgtatcag
     2701 gttcgggcaa atcaacttta gttaatgaca cactattccc tctggcacag aatgcgttaa
     2761 accgagcgga aaacagcgat gttgctcctt atgaatcgat tgacggctta tcgcattttg
     2821 acaaggtaat tgatattgac caaagcccga ttgggagaac tcctcgctca aatccggcaa
     2881 cttacaccgg attatttacg ccaattcgtg agatttttgc cggcacgcaa gaagctcgag
     2941 ccagagggta taacgtaggg cggtttagct ttaatgtgcg gggcggacgt tgcgaagcct
     3001 gtcaaggtga cggcgtgatt aaagttgaaa tgcacttctt gcctgatgtg tatgtgcctt
     3061 gcgatcactg taaaggcaaa cgctataacc gtgaaacctt ggaaattcgc tataaaggca
     3121 aaactattca ccaagtgtta gagatgacgg tggaagaggc gagagaattt tttgccccta
     3181 tgccgatgat tgcccgcaag ttgcaaacct tgatggacgt ggggttatct tatatccgtt
     3241 tagggcaatc ttctactacg ctctcaggcg gtgaagccca acgggtgaaa ttggcgaccg
     3301 agctttccaa acgagacacc ggcaaaacgc tctatatttt agatgaaccg accaccggct
     3361 tgcattttgc cgatattaaa cagttactcg gcgtgctaca tcgcctgcgt gatcaaggca
     3421 atacgattgt ggtgatcgaa cataatttag atgtgattaa aaccgcagac tggattgtcg
     3481 atcttggccc tgaaggtggc agtggcggcg gtgaaattat tgctaccggc acaccggaag
     3541 aagtggcaaa agacaaacgt tctcacaccg cacgcttctt aaaagagatt ttggctaaag
     3601 gctaacgttc aacacaagcg gtcatatttt cggaaaatgt tgcaatttgc aaaaagttgg
     3661 aaaatatgac cgcttgtttt atatccggag ttattcctag ttagcctgtc tgattttgtc
     3721 gaaggcagat tcaagatcgc tactgaattg aatttgcggg atttcgttta ccgttttttg
     3781 tgagcggact agcattctca tcggctgaaa ttgcacatta cagagatgaa gttgttgatg
     3841 tggcaacata tgctgtacaa agcgggttag ggcgtgaatg ccaccagcat ctaatactgt
     3901 aacggcatcg cattgcagca caatatgttt aatttggtga tcggtatgta cggttttatt
     3961 gtgcagatcg gcaaagagat tatcggcggc agcaaaaaat aacggaccgc tgatccgata
     4021 aaccaacaca tcgttcaaat cggttgggtg agccatttca aaggatttag tcatttctgc
     4081 aatagtacgg ataaatagca ggcttgccag taacacccct accgtaatgg caattaccat
     4141 atcaaataat accgtgaggg taatgcaagt cagtaaaacc gcaatttcat ttttacctga
     4201 acggcgggta agttgaatga tttgtggaat atctgccata ttccaagcta ctaccaataa
     4261 tagtgcagcc attgaggaaa gaggcaaata ggaaagtgct ttagccaaaa agagtagggc
     4321 aaataaaacc aacaaggcgt gaaccacact agaaatgggg gaaactgccc cggaacgaac
     4381 attggcagcc gatctcgcaa tcgctgctgt ggcggtaatt ccccctaaaa atggcgaggc
     4441 aatattacct aagccttggg ctaatagttc gttgttagag tggtgcttgg tatctgtcat
     4501 attatcgcac actaccgcac ataataacga ttcgatagcc ccaagcattg ccattgaaaa
     4561 ggcagccggt aataaggctt gaatggtggt taagttccaa tctatccgat tcccctctgc
     4621 attcgggaga ttccaaggta acacaaattc aggtaatacg cttgggatac catacccagt
     4681 ggtgccgtca gagagcgtgt agtggaatgc tgagccgata gtggcaacgt tttgtccgaa
     4741 atgaatcagc agtaatgata acagcgtgcc gataaagacg gcgggtaaat gcccgggaat
     4801 aggcaaccgt agcttgtgcc aacgagttaa aaccaacaat gttacgacac ccactaaggt
     4861 gtcagcccaa tttatggttg gcaatgctga aaaaatagcc tctagcttgc caagatagtg
     4921 ttcaggcatt ttttcaatgc ttaagcccag aaaatctttg atttgcaagg tggcgatcac
     4981 aataccgatg ccacaggtaa aacccagtgt gacaggtaga ggaatgtatt caattaagcg
     5041 tcctaatcgg agtaacgcca tcaatactaa aattcctccg gaaagcattg ttgccatgag
     5101 taatccactt aagccaaact gctgcgtgac ggggtataga atcaccacaa aagcggctgt
     5161 tgggcctgaa atattaaatc ttgaaccgcc tgttagggca atcacaatcc cggcaacaat
     5221 cgccgtatat aaaccgtgct gtggtggcac accgctggca atcgccaacg ccatagataa
     5281 cggaatagca ataacgccaa cggttaagcc tgaaataata tctctgatta aatgttgttt
     5341 accataacct gcattgagag catcttttaa tgcactaaaa ggcttgacgg caagaaaagc
     5401 atttttagtt aaaaaccatt tatttaacat agaatgttcg agtgaagaga atgtttcgct
     5461 tattttactg caatgaataa aattaaattt gagctaacgc aagaaaaagc aggaaagacg
     5521 aaatatgttg gagacaagcg gttgttttag gcgaaaaatt tgcaaataca aataattagc
     5581 tgattttatt ttgaaaatag cgtaaaatgc cactttaatt tgtactacta tagtagtgca
     5641 tttgtgtgat ttcattaact ataataaaca aatggacagt cttattcatt ttttgagtgc
     5701 ctttttaact aatatcactt ggattggggc gttagagctg gggttaattt attcccttgt
     5761 tgcccttggg gtattaattt cttataaaat tctggatttt ccggatctga ccgcagacgg
     5821 cagtttccct ttaggagggg cggtgtgtgt gatcagcatt ttgaatcaag ttgatccttg
     5881 gctggcaact ttatacggca cgcttgccgg agcagtagcc ggtatggtta ctgcaagttt
     5941 gcatatcggc tttaaaatcg aaaaattgct tgccagtatt ttaatgatga tcgcactcta
     6001 ttcgattaat ctacgcatta tgggaatgcc gaatattgct attttaggcg aaaccacgat
     6061 ttacgatgta atgccgattg aaagcgatct acagttagcc ttagtacgat tagtgatggc
     6121 attgctggtt gttattatcg taaaaggttt gtttgacctc ttttttgcga ccgaaatcgg
     6181 tttggctgtg cgggcaaccg gtactaatag tcgaatggcg aaagcacagg gtattgcgat
     6241 taataaaatg acgattttag ggatggcgat ttctaatgcg ttaattgcct taggtggtgc
     6301 tttgtatgtg caaagcaacg gtggtgtaga tatttcgatt ggggtaggaa ctatcgtgat
     6361 tggcttggcg gcggtaatta tcggggaagc aattttttcg gtaaaacgga ttatttggct
     6421 tacctttgcg gtgattattg gctcaattct ttatcgtttc ttcatcgcac tagcattaaa
     6481 taacgaaact ttaaacggca ttggctttgg cccgcaagat ctgaatttaa ttacggcgtt
     6541 attggtggtg attgtattag cggttcctcg aattaaacac aaattgctcg gtaagcgagg
     6601 ttaatatgat tgaactcaat aacttattta ttaccttcaa caaagggaca gcaattgaga
     6661 accctgtgct gagaggctta tccctaaaag ttgagcaagg cgaatttgtg tcggtaattg
     6721 gtagcaatgg ggcgggtaaa tctaccttat taaatgcgat cagtggtgat tgtgcggtgg
     6781 actccggtga gattttaatt caaggaaata aggtaaacca atccaccact tggcaacggg
     6841 caaatcaagt cgcccgagtt ttccaagacc cgatggctgg cacttgtgaa agtttaacga
     6901 ttgaagaaaa tatggcactg gcttaccaac gtggacgtaa acgtgggttg ggctttgcga
     6961 tcaaacgcca aatgcgagag ttattcaaag acaaacttgc tttgttaggg ctaggtttgg
     7021 aaaatcggct tactgatcaa atggggcgtt tatcaggcgg gcaacgccaa gcggtgagct
     7081 tgctgatggc atcacttcag ccgtctaata tcttattgtt agatgaacac accgcggcac
     7141 tggatccgaa aaccaccgat tttgtgcttg aacttaccaa taaaattgta agagaacaga
     7201 aattaaccac gctaatggta acgcattcaa tgcggcaagc ccttgactat ggcgatagaa
     7261 ccgtgatgtt acaccaaggg caagtggcgt ttgatgtttc gggcgagcaa cggcaaaaaa
     7321 tggacgtgcc ggatctgtta caacttttcc aacaaagtcg ccacgagcag ttgagtgacg
     7381 atggattatt actcggcaat taattttgcg atctgcatcg aaaaaatgaa tttctccttt
     7441 tgtttttggc tgtcaaattt taaataataa gcccaaaagg aggtattcaa tgttaagcat
     7501 tttcaaaaca ccggtagaga aagaaacgct agacgattgg gctaaaattt cggttgatgt
     7561 agcgaaagtt gcgattttgg caattcctgt tatacttttt ggcaaagaac caacgttgat
     7621 taaattgact aacttagcat tattggtatt gagtatttat gtttttttga cgcttgggcg
     7681 aaaattacgg cagctaaagg aggtgttgtg atggaactag gctggaattt aaccataggt
     7741 ttgctaattt taaacgtctt tgcgtttgct tggctttata ttattaaccg gatttctaaa
     7801 gaacaattat ccaaaaatca ataatattta atttttcaca agcggttgtt tttgcaaaat
     7861 attttgtaaa ttcgaccgct tgcctgtttt taaatgacaa aatacgacga taaaattcag
     7921 catgcattta gcgaaaatgg gttgctcagc caaaatatca gtggatttcg accacgccaa
     7981 gcacagcttg aaatggctca agtggtcgca aaagcagtaa aatttgcgac acctgttgtg
     8041 gtagaggcgg gaaccggcac aggcaaaacc tttgcttatc ttgtgccggc actactttca
     8101 ggcaagaaaa ctattctttc gacaggttct aaaaacctgc aagatcaact ttttaatcga
     8161 gatcttccca ctattcaaaa agccctgaaa tacaaaggca aaattgcgtt actcaaaggg
     8221 cgtgcgaact atttgtgttt agagcgttta gatcaagtga ccgcaatggg cgtacttggg
     8281 gataaatccg ttcttaccga tttaaaaaaa gtcagtaaat ggcaaaatgc cactaaaaca
     8341 ggggatttaa gtgaatgtat taccattgcc gaagatagcc cgattctacc gcagttagtt
     8401 agcaccaccg agagttgctt aggctcggac tgcccgcatt ttaaagattg ttatgtggta
     8461 caagcccgcc gtaaagcgat ggaggcagac gtagtggtgg taaaccatca cctattctgt
     8521 gctgatatgg cagtaaaaaa gaccggtttt ggtgagttga tccctgaagc tgagctggtg
     8581 atctttgatg aagctcacca actgccggat attgccagcc aatattttgg gcaatcgctt
     8641 tcttcccgcc agttatttga tatttgtaag gataccaata ttgtttaccg cactgaactc
     8701 aaagatctgt ctcaattagg caaagcggct gatcacttga cgaaagtagt gcaagatttc
     8761 cgattattga tgggcggaga agggcagatt cgaggcaatt tgcgagagtt atttcgtgat
     8821 aaaaaagtgg tcgatggctt ggtaaaagtg gctgaaaata ttgatttttt aagtgaagtg
     8881 gcgaaaaaat cgctggggcg gtcggaaacg ttggataaaa ttttcgagcg gttagcagaa
     8941 gttaaggttc aacttaaaaa attaatggat actagtgtga caggctattg ttactggtat
     9001 gaggcgaatg gacgatcctt tgggctgcat attacgccgc ttatggtggc agataaattt
     9061 ggtgaacagt tgaagaacca gcaaattggt tgggtgttta cctcagccac actcgaagtt
     9121 gggggcaaat tcgatcattt ttgtcaacgg ctgggcatcg aaaatgcgga gcagttggtg
     9181 ttacaaagtc cgtttgatta ccaacatcaa tctttactct gtgtgccccg ctacttgccg
     9241 gataccaata aatccaatac tttaactgct ctcgggcaat tattaaaacc ggtgattgag
     9301 gcgaataaag ggcgttgttt cttgctttgt acttcctatt atatgatgcg aggtttagcc
     9361 gactttttac gcgaacacag ttcgctgaat gtgttattgc aaggcgaaac cagcaaaagc
     9421 cgtttattgg aaaaatttgt gaaagaagat cacagcgtgt tggtggcgac ccaaagtttt
     9481 tgggagggga ttgatgtgcg aggcgatgat ttatcgttgg tgattattga taaattgccg
     9541 ttcacttcac cggacgaacc attactcaaa gcccgaatgg aagattgtaa gatacagggc
     9601 ggtgatccgt ttaacgatat tcaaattccc gaagcggtga ttaccctcaa acaaggtgta
     9661 gggcgtttaa ttcgtgatgt gaccgataga ggtgtagtga ttatttgtga ttcccgtttg
     9721 gtgatgcgaa attatgggca aactttcttg aaaagtttac cgccgtctag acgcactaga
     9781 gatttgggga atgttattca atttttgaaa caaaaatagg gttacaagcg gtcagatttc
     9841 tgcagatttt tgcaaaaaaa cggataaaaa taaccgcttg ttgaggagtt atatgttgtc
     9901 tacattgctg cgtatggttg cttctttggc attgttattt gtgatgcttt atttaggcaa
     9961 gttaatcgtt tatctattcc ccatcgggat cccggatagt attttaggta tgcttatctt
    10021 attcggctgc ttggttatcg gcattgttaa agtggaatgg gtgataccga gcgcgaaacc
    10081 gctcacacgt tatataacac tatttttcct tccgatttgt gtcgaactca tcgaacatct
    10141 tgagctgtta cgccaaaatc tgaacacttt tgtattagca acggcattaa gcaccttagt
    10201 ttctgtggtc gcgattggcg tgtttgcaca gtggattttt cattacaaga gagggtaaaa
    10261 tgagctatct ttacactgtg ttgacggtaa tcatgtttat tgccggtttg aaaatcagcc
    10321 aaaaactcaa gagcagtttt ctgaaccctt tcttaattgc actcgctttg ctaattggtg
    10381 tattactgat ttttaatatc ccttaccaag actattatca aggtaattcc cccttgaata
    10441 acttacttgg cgtttctgtt gtggcacttg cttacccttt ttatgaacaa ttaccccata
    10501 tccgtaaaaa atggcgacag attgtcgtga ttttgctgtt tagtaccatc attacgatga
    10561 taaccgggct tgggttttca ttattatttg gggcaagcca agagatctta gccgccattt
    10621 tgcctaaatc ggtatcaatg ccaatggcaa ttattattac gaatgaaatt aatggaaatg
    10681 ttgcaatcgc agcggttggg gtgatgatcg caggcttagt gggatctatt ttaggcttta
    10741 cgcttctgcg cctggcaaaa gtaagaaatg tgatggtgat tgggttaagt atgggagctg
    10801 cttcccacgc attaggcacg gcaagatgct tagagcatag tatgaaagcc ggtagctata
    10861 gttctatcgc attagtggct tgtggtgtgc tgtcttctat atttgcacct ttaatattta
    10921 acgttatcgt cacgttatgt tactaaaaac agtgaaatct caaaccttat ttggtaactg
    10981 gctgaaatca aggtacaata ccgtgatttt tcaatgaaaa tagagaatta tgcaagaaca
    11041 aataacacaa gaaaaagatc tttctacgct taaaaccttt aagcgtttat ggccgactat
    11101 cagcccgttt aaagcgggtc tgattacagg tgctgtcgct ttagtattaa atgcgttagt
    11161 ggattctagt cttatctatc tcctcaaacc cttgctagat gaaggctttg ggcaagctaa
    11221 taatgatttt ctgaaattga tggcggtgct tgtagtagtt tttattttag tgcgcggtat
    11281 taccaattat attgccagtt actgtttagc ttgggtgtca ggcaaggtgg taatgacatt
    11341 acgccgccat atttttcaac atttaatgta tatgccggta agctattttg ataacaaccc
    11401 gaccgggcgt ttgctctcac gagtgactta cgataccgaa atggtggcga gttctgcttc
    11461 tcattcttta gtgactattg tgcgtgaagg ggcttatctg ctgtcattgt ttgtggtgat
    11521 gatctatacc agttggcagc tttctattgt gttatttgtg atggctccta tcattggggt
    11581 actgatcagt ttcgtttcaa aatattttcg tcaattaagt cgcaatatcc aaagttcaat
    11641 gggggagctt actgtcacga cagaacaaat gcttaaaggg cataaggttg tactttcctt
    11701 tggtgggcag gaaattgaga aagcacgttt tgatcgagtc agtaatgata tgcgtcgtaa
    11761 agggatgaaa gtcgtttctg ctgatggcat ttctgatggt gtggtacagc ttatcgcctc
    11821 ttttgcgtta tctgctgtgc tttatgttgc gaccttcccc gaggtgatga gtgagaattt
    11881 aaccgcaggt tcattcacag tagtgttttc ttcgatgatg gcaatgttac gtcctctaaa
    11941 atcactcacc aatgtgaact cgcaattcca acgtggaatg gcagcctgcc aaacgttatt
    12001 tgaattttta gatttaaaaa ctgaaaaaga tcacggctta atctccgttg ctaaagctga
    12061 aggcaatgtg gaatttaaaa aggtaagctt tagttatgaa ggtaaagagg aaaaagcact
    12121 cgataatatt tctttcacca ttcctaaagg caaaacagta gcattggttg ggcgttccgg
    12181 ctcgggtaaa tcaactattg ccaatttgct tacccgtttt tatgatgtga ctgacggtca
    12241 aattttgctt gatggaatta atattcaaga ttaccgctta aaaaacttaa gagaacaatg
    12301 ctcggtggtt tctcaacaag ttcatttgtt taacgatact attgctaata atattgctta
    12361 tgctgcaatc gacaaataca gccgagaaga aattattgct gctgcaaaag ctgcacacgc
    12421 aatggaattt atcgaaaaat tggaaaatgg cttagacact gtaattggtg aaaatggcgc
    12481 aagtctttcc ggtgggcaac gccaacgctt ggcaatcgct cgtgcattac tgcgaaattc
    12541 tcctgtgctt gtgctggatg aagctacctc tgcattagat acggagtcag aaagagcgat
    12601 ccaatctgca cttgaagtgc tacagaaaga tcgtacggtg ttggtcatag cacaccggtt
    12661 atcaacgatt gaaaaagccg atgaaatttt ggtagtcgat cacggaaata ttattgagcg
    12721 tggcagccat acggaattgt tagcacaagg tggggcttat aaacaactcc atagtttaca
    12781 attcggagcc taatttgtag gggagaaata tgtttctccc caattaatga taaaaacagg
    12841 gcgaaaaata tttcgtccct acaataattt atttggatac tatgaaaaaa ctgacaaaac
    12901 ttttatctct tagtgcttta ctggtcttaa ccgcctgtaa ttcggtacta aatgcctcaa
    12961 cagaggtgca acaaccaaca gtacaaattc cgcatgacga tccacaatgg cagcagcatt
    13021 tagcgaaact gagccaaatt caaggctata ctgcaacggg gcaatttggc tatatttcgc
    13081 ctgaagagcg tttttcttcc catttcaact ggcgttatac taatccaatc caatttgggt
    13141 tagagctttc ctcgaatctc tcgactaaat cactgaaatt agagcgtaat aaacgaggct
    13201 taacggtttc ggacaatgag ggtaatagcc gttctgaccg tgatgttgat gcgttaatgc
    13261 aagagattat cggtgtttcg tttcctatcg atcagtttgc ctattgggta aaaggcttgc
    13321 ctgaaaaaga tggcaactat attgtgaatg aaaaacgcca gctgtcgcaa tttagttatc
    13381 cgcttaatgg cacgctttgg aaagcaagct atgtggagta tcacgaagat cgtgtaccaa
    13441 acttaccgaa gctgattgtg cttgaaaacg gcactcaaac gttgaagatc cgcattgaaa
    13501 aatgggcgta ttaatgaaga aagtcacctt acccagcccg gcaaaattaa acctgttttt
    13561 atacattacc aataagcggg cagacggtta tcacgagcta caaacgctgt ttcagttttt
    13621 agattttggt gatgaaattg agattgagat cacagaaagt tcagaggttg aattaattaa
    13681 tccggtagaa ggggtggcaa ctgaggagaa tctcatttac cgagcagcaa aacttttgca
    13741 aaaaacgacc gcttgtaggc aaggctgccg tattgggatt aaaaaaaacc taccgatggg
    13801 cggaggtgtt ggtggtggat cttctaatgc tgcaacagta ttagtcggtt taaatcactt
    13861 gtggaatacc gagctttcat tggaaaaatt ggcagaatta ggcttaagtt taggggctga
    13921 tgtgccgata tttgttagag gtatggctgc ttttgccgaa ggcgtggggg aggtgttaac
    13981 tccttgtgat ccacaagaaa aatggtatgt agtgctaaaa ccagaagtgt cgatttcaac
    14041 cgcagcggtg tttaccgacc cgaatttacc tagaaatacg ccaaaacgcc ccttagccga
    14101 attattatct ttaccgtggg aaaacgattg cgaaaaagtt gtacgagaaa agtattctgc
    14161 ggttgaagat cttatacaag aattgttaca atatgctcag tttaggctaa caggcacagg
    14221 tgcttgtatt ttcgccgaat ttgaaacaaa agcagaagcc gagcaggttt ttgcacataa
    14281 accggaagcc gtcttcggtt ttgtggcgaa agggcaaaat atttcgccat tacatcaaat
    14341 gttattccaa atctaaccat cttaacctta aggatcaatc catgacagat attaaactgt
    14401 ttgcgggcaa cgcaactccg gaacttgcta atcgtattgc aaaacatctt tatacctcat
    14461 taggtaatgc aacagttggt cgttttagcg atggtgaaat tcaagtacaa atcaatgaaa
    14521 atgtgcgtgg aggcgatatt tttattgtgc aatccacttg tgcaccaact aatgataatt
    14581 taatggaatt gatcgtgatg gtagatgcgc ttcgccgtgc atcagctggt cgtattaccg
    14641 cagtgattcc gtatttcggt tatgcacgcc aagacagacg cgtgcgttcg gcccgtgtgc
    14701 caattaccgc aaaagtgatt gctgatttct tatccagcgt aggggtagat agagtcttaa
    14761 cctgcgactt acacgcagag caaattcaag gtttctttga tgtgccggta gataacgtat
    14821 ttggttcccc tgtgttaatc gatgatattt tgaaaaaatc cgatttggtc aaccctatcg
    14881 tggtttctcc agatattggt ggggttgttc gtgcaagagc ggtggcaaaa ttattaaatg
    14941 ataccgatat ggcaattatt gataaacgtc gtccaaaagc gaatgtatca caggtaatgc
    15001 acattatcgg tgatgtggct gatcgtgact gtattttagt tgatgatatg atcgatactg
    15061 gtggtacttt agtcaaagcg gctgaagcgc tgaaagaacg tggcgcccgt cgtgtctttg
    15121 cttatgccac acacgcggta ttttcaggca cagcagctca aaatttagcg aatcctgctt
    15181 tagatgaaat tgtagtaact gatactattc cactgagtaa tgagattaaa gcattaaata
    15241 aagtacgtgt tttaacatta tccggtatgt tagcagaagc aattcgccgt attagcaatg
    15301 aagaatccat ttcagcaatg tttgatgcct aatttatgag aaaaaaccca gtcgaaagat
    15361 tgggtttttc atatctgttc actactatat ttagtttaag ataatttgta tgtattccct
    15421 gattcgtaaa ttactttttg cactagatac ggaaaaagca catcaattct ctattcaggc
    15481 acttgcttta gccggaaaat cgcctttttc gattctgcca acacccgata atccgaccca
    15541 agtaatggga ctcacgttta aaaatccaat agggcttgca gccggagcgg ataaaaacgg
    15601 tgaagcgata gatggttttg ggaaattagg atttgggttt attgaggttg ggactattac
    15661 tcctctcgct caagacggaa accctaaacc gaggcaattt cgtattgtag atgcagaggg
    15721 aattattaac cgcaatggat ttaataataa aggcgttgat gttttagtag aaaatgtaaa
    15781 aaatgcaacc tatagtggta ttttaggtat taatattgga aaaaatgcgg ttacaccaat
    15841 tgagcatagc tttgatgact accaaatttg tttgcgtaag gtttaccaat atgctgatta
    15901 tgtggcggtg aatatttcat cacccaatac caaaaatttg cgcagtttac aatatggtga
    15961 ggcattagat gatttgctgc gaaacctcaa aaatgagcag gcacaacttt cgcaaaaaat
    16021 ggggaaatat cgaccgcttg ttttaaagat tgcgcctgat ttaaccgatg aagaaatcgt
    16081 ttctgtagcc gatagtctta ttcgttacca aattgatggg gtgattgccg gcaatactac
    16141 cttgtctcgt gaaagtattg ccggattacc gtattcggaa caactgggtg gattaagtgg
    16201 caagccatta cactcattaa gtacaaggtt aatttcaaaa ttatctgccg agttgggcga
    16261 gagaatacca attataggtt gtgggggagt ccattcgtta gagagtgggc aggagaaagt
    16321 gaatgcaggt gctagtttat tgcaagtcta ttccgcaatg atttaccaag gaccatcatt
    16381 aattcaaacc ttagccaaac accttcagct gaacagttag ggatagattt ctagttaaaa
    16441 atgattggtt aggtttattt ctgtcatctt agtggaatag atggcggtat tagctatcta
    16501 tcaaaaatag acaaaatt
//
LOCUS       DANLRK010000041        16516 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.041, whole genome shotgun sequence.
ACCESSION   DANLRK010000041 DANLRK010000000
VERSION     DANLRK010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 16516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16516
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.041"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(<1..895)
                     /locus_tag="PXA33_000938"
     CDS             complement(<1..895)
                     /locus_tag="PXA33_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL5120284.1"
                     /translation="MSKIEFEKIPNSIRKPSVLTEYNNKDAVTTLPTNEQEVLIVAPM
                     LNGEAAFTAPQKIFSDVEAENLFGKGSVAHLMVRQAIQNNPLIRLTVVGLKDHEAGIA
                     ATGQVSFTGTVTYAGVVRITIAGTAYEVAAAKGEEAQAIVARLVNVINAASYSPVVAS
                     VESETTLKLTSKAKGEISNEITLATRNTATGLTLEARAFDNGQRNALIAPALASVAGT
                     HYNVIISPFSDDENALALRSHLESVSAPIEDKPAIGVMGWRGTYATGTTLTASLNSER
                     IIVGWYKGATESNAMIAAGLGA"
     gene            complement(895..1074)
                     /locus_tag="PXA33_000939"
     CDS             complement(895..1074)
                     /locus_tag="PXA33_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HDL5120285.1"
                     /translation="MLKVKAAKGVRVPVENRPHHYIEQEAVEVENTLYYQRRIADGDL
                     IVVKETKSAKETGGK"
     gene            complement(1075..1716)
                     /locus_tag="PXA33_000940"
     CDS             complement(1075..1716)
                     /locus_tag="PXA33_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005822889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1834 family protein"
                     /protein_id="HDL5120286.1"
                     /translation="MISKIERALIERLTLGLGKMVYAVAGYAGEINDEKLDVLRLPAC
                     LVSYGGSAFEAKSMGVRGKRYQATDVFVVLVITRSMRNQTAGRLGGVTSREVGVNQLL
                     GAVKTLLINQTLDGLVHPIQPKRIRTIWNNAEVKKEKLSAYAIEFEISYNECNVLDDG
                     RFPEGVEPNEQVFKQYQGKLDEPQGDLSGFNNRIFDPNNNAETAFRTEFKDGD"
     gene            complement(1713..2138)
                     /locus_tag="PXA33_000941"
     CDS             complement(1713..2138)
                     /locus_tag="PXA33_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1320 domain-containing protein"
                     /protein_id="HDL5120287.1"
                     /translation="MYALVDDFVLRIGEQESIELTDREMVGVVDEATLEVALADSSSQ
                     IDGYLSGRYRLPLKTVPQNLKRICCDLARYHLTSKSLVTMTEEVENRYKLCLKELENI
                     SKGVVSLAVDETNEEESATGENTVQFFNGGNRIWGRDKR"
     gene            complement(2138..2479)
                     /locus_tag="PXA33_000942"
     CDS             complement(2138..2479)
                     /locus_tag="PXA33_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005822892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5120288.1"
                     /translation="MAKPKKNDNEALNTNENTPLEDKVNEQPEDKTDEQPKPPESETK
                     PDDGALINPIAFEVKLKSIHPQATYGRCGYRFNKETAVEIARKALSDEQIITLANDPY
                     LEFVPVVEGDA"
     gene            complement(2527..3444)
                     /locus_tag="PXA33_000943"
     CDS             complement(2527..3444)
                     /locus_tag="PXA33_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu-like prophage major head subunit gpT family
                     protein"
                     /protein_id="HDL5120289.1"
                     /translation="MFKKSELLKALDTAFRKEFSAGLKVLEPQWGTVAMKVSSSTATN
                     TYAWLGQFPKMQEWVGDRQIKNMQAQGMTIENKLFESTVAVPRTAIEDDQVGLFTPMV
                     KQAAQSAAELPDDLVFSLLKKGKTTLCYDGQNFFDTDHPVYQNVDGTGTSKTQSNITT
                     GSASGKPAFYVLDDSQAIKPLIWQERTTPEIETKFDPSESDHVFMKDQYLWGVRARGN
                     AGFGFWQLIHRVEDSELNSETLEKVLTAMRTLKGDGDKQLNIRPTTLLVPPSLEFAAR
                     KLLEAELIDGTTNTLKGVLKVVVNPFIAE"
     gene            complement(3444..4514)
                     /locus_tag="PXA33_000944"
     CDS             complement(3444..4514)
                     /locus_tag="PXA33_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005606544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protease"
                     /protein_id="HDL5120290.1"
                     /translation="MFKHKFKLNPIALSFKLNQEANGRIQLFPFGWFEAQDGRGGRWY
                     VGDENGYALADDINNTAIDLMIDYEHQTLYIAENGKGNPAAGWITKAEYISGEGLFAD
                     VKWTPQATQEIKDGVYRYLSPLFLTDGSGMVIKVLNAALTNRPALHNLQEVVAMSSQF
                     AKFLTPEEENPKMKELLIKLFALSANATDEQITEKLTALEKAKGDSPVALSDVYTELA
                     KEKSQVVALSAKVNHPDPAKFVALSDLQAVQTELNGLKQQINDKERDALIQTALSDGR
                     LLPAQKAWAEKLGKQDLVALSDFLKTVTPNPALASTQSGGEDPNKGTEKVALSAAEIA
                     AAKSLGLSEAAYIEKYKTEESK"
     gene            complement(4772..5188)
                     /locus_tag="PXA33_000945"
     CDS             complement(4772..5188)
                     /locus_tag="PXA33_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HDL5120291.1"
                     /translation="MIEIQIHGIDEIIATLNRIAAKTANKTDLMRNIAGKMESAVLTN
                     FDEGGRPKWLGIKHRQGTPLVDTENLMGSITADYNNDSAIVGTNEPYAAIHQFGGMAG
                     RGRKVKIEARPFLQLTSQDRQDILDDVQAYWQKLLD"
     gene            complement(5339..6638)
                     /locus_tag="PXA33_000946"
                     /pseudo
     CDS             complement(5339..6638)
                     /locus_tag="PXA33_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248654.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
     gene            complement(6625..8250)
                     /locus_tag="PXA33_000947"
     CDS             complement(6625..8250)
                     /locus_tag="PXA33_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 domain-containing protein"
                     /protein_id="HDL5120292.1"
                     /translation="MSKKKHNKQIKPNLNPIKNQELQTDLAEITATGRVLSDHPSNFI
                     TPAKMKAIFEDTENGDITAQHELFMDIEERDSSIFANIQTRKRAALGVDWAIVAPRNA
                     TPQEEKLRDEVDELFYQLGNLEDLVMDCMDAVGHGFAALEIEWAFNGKLWYPNAFIHR
                     PQSWFKWDKHDNLLLKTRQNTEGEPLREFGWVVHTHKSRSTQAARNNLFRSLAWLYMF
                     KHYSIHDFAEFLELYGMPIRIGKYGAGATPNEKQTLKRALAEIGHNAAGIMPESMSIE
                     LHNAANAGGAAGNNPFLQMVDWCEKSIARLILGQTLTSGADGKSSTNALGNVHNEVRR
                     DLLVSDVKQLGQTFTQQIILPFLQINFGNIDPTRIPSFEFDTKEPADLALFAESLPKL
                     VDIGMPIPVQWAMDKLGIPEAQENEAVLGRSAPPQAVGLSANFPQKMTACECGCGGKV
                     HALSAQTKTGIKEQDALDDLADEAFSVPDFNAQFDPITKKAVAVVMACSSYDEAAEKL
                     AAAYPDLVSEEHDRYLANALFLADLLGAANAES"
     gene            complement(8319..9944)
                     /gene="terL"
                     /locus_tag="PXA33_000948"
     CDS             complement(8319..9944)
                     /gene="terL"
                     /locus_tag="PXA33_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HDL5120293.1"
                     /translation="MKIKEFEKELEELRRQLQQNIEASFEGWDDKPQAISERRQKVLD
                     PVHGFEYFVQAYFPHYVRSEHKSQLHHYLFENLPLSVSDLSKSVRQAIAAPRGEAKST
                     ICTQLFPLWCLVRDLKRYIIIAMDTKEQAYSMLEAIKVEIESNPRLRIDFPEVAQGKV
                     WRAGVIMTAKKQKVEAVGAGQKLRGRRHGAYRPDLVVLDDIENDESVETPAQRNKLHN
                     WVLKAVLKLGAAGEKFDVIYVGTILHYDSVLSRILNTKGWRRVRFKAILRFPGNMALW
                     DEWENIYLSEEGDDDTMSDLFYQQHKAEMDKGALVSWLARPILYLMKIRASDGHSAFD
                     SEYQNDPVSGDDAIFANSIQYWTELPDDLIYFGALDPSMGKAGASRDPSAILVGGYHR
                     ESGKLYVVEAQIKKRLPDLIIEDVIRLHSQYNCHRWFVETVQFQEFLQTELVKRSAQR
                     GKPVPATATKPNSDKMLRIESLQPHIANGLILLHRSQSTLESQLRHFPKADHDDGPDA
                     LEMLWRNALSSSAPIEWESVDDFDGEYRSKWRH"
     gene            complement(9948..10094)
                     /locus_tag="PXA33_000949"
     CDS             complement(9948..10094)
                     /locus_tag="PXA33_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5120294.1"
                     /translation="MAKKKQTVVHKHYSFRWIVQLACIFGAIKLMLNGTDGWGWLLLI
                     AACM"
     gene            complement(10094..10594)
                     /locus_tag="PXA33_000950"
     CDS             complement(10094..10594)
                     /locus_tag="PXA33_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1804 family protein"
                     /protein_id="HDL5120295.1"
                     /translation="MAHDVEVQKAVRQAYVFDRLSLEMAAERAGVSFGTARRWKAAAE
                     KNGDNWEKARDVQAMASGGIENIAQGLLAGFLIKYRTLMTELEENTEMPTAAKVEALA
                     ALADSFAKMTASSKKLLPETSAMATAMRAIEMMANIVKTKKPHLLPDFLEMLDELEVQ
                     FKKEFK"
     gene            complement(10602..10856)
                     /locus_tag="PXA33_000951"
     CDS             complement(10602..10856)
                     /locus_tag="PXA33_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005606393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5120296.1"
                     /translation="MSRKKQKQTDSEKLDLILGGLNELNDKVDKQNEEIERLHREIQA
                     TNRLVNEMARKNRRDAVVAGGLGGGIVAVGFELLRLKFGM"
     gene            complement(10856..11113)
                     /locus_tag="PXA33_000952"
     CDS             complement(10856..11113)
                     /locus_tag="PXA33_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005822915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5120297.1"
                     /translation="MIDEKLFQMLISFVVAPLVAFVGKVIFDRINRNEQTVKELKAEM
                     ESKYQSKELAHEVNRGIKEKLDDILDSLKEVNQKLDKKADK"
     gene            complement(11106..11261)
                     /locus_tag="PXA33_000953"
     CDS             complement(11106..11261)
                     /locus_tag="PXA33_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5120298.1"
                     /translation="MKLIKKLRQRWQQWRFYRRRPDLAELHNVVRAAVAQGNQHPVGR
                     AKRGRYD"
     gene            complement(11276..11635)
                     /locus_tag="PXA33_000954"
     CDS             complement(11276..11635)
                     /locus_tag="PXA33_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2681 domain-containing protein"
                     /protein_id="HDL5120299.1"
                     /translation="MITQQIAGVVGVVSTILAYAFYKSWQLKQERKLTATLKAEKQQQ
                     AVEIQQKNAEVKNAKIQQKHVSETRRIQPSAIDEQLHQHGYFRDDNGLHGVRSDLPEP
                     CGHNGNETPSACTQSDL"
     gene            complement(11632..11877)
                     /locus_tag="PXA33_000955"
     CDS             complement(11632..11877)
                     /locus_tag="PXA33_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2644 domain-containing protein"
                     /protein_id="HDL5120300.1"
                     /translation="MKQFLLDLITNKDGTASTTGFIQFMSWLVLSGILIHAYLYDKPF
                     ISDWWFAYAGICVLGSPATKGVVSALKQKERSREAEE"
     gene            complement(11880..12413)
                     /locus_tag="PXA33_000956"
     CDS             complement(11880..12413)
                     /locus_tag="PXA33_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 108 protein"
                     /protein_id="HDL5120301.1"
                     /translation="MTEPIKNKATIADFNLAFDRLIGHEGGYVNDPKDPGGETNWGIT
                     IHTARANGYTGSMRAMSRADAKTIYLKAFWQRYNCEQFPPELAFQFLDACVNHGSGNA
                     SRMLQRAVGVADDGIIGKVTLAAIERYSVADVGLLFQAERLEFYTKLKHFDRFGRGWI
                     NRMAGNLRYFAKDTVEE"
     gene            complement(12499..12924)
                     /locus_tag="PXA33_000957"
     CDS             complement(12499..12924)
                     /locus_tag="PXA33_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005822923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mor transcription activator family protein"
                     /protein_id="HDL5120302.1"
                     /translation="MSEFESVEHYLPETVKEIVGVIGLPATEKLIKAFGGFSFQFSNG
                     KLYFNKLKEVLGQDDAVKLQAYMGACEVYLPRCETALRMLRNQQIYADYCQLTEQGGL
                     SGRLAIMQICPKYSVCDRVAWEAVRYYQRKHTVSQATLF"
     gene            complement(12995..13210)
                     /locus_tag="PXA33_000958"
     CDS             complement(12995..13210)
                     /locus_tag="PXA33_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL5120303.1"
                     /translation="MIENPEFGYTPSNLKMLRKHHNLTQTEVANILGVGQRIVMRWET
                     ELSSTAARSDMPYTKWAELLKNLQKNF"
     gene            complement(13229..13621)
                     /locus_tag="PXA33_000959"
     CDS             complement(13229..13621)
                     /locus_tag="PXA33_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5120304.1"
                     /translation="MTDYQKLLNALLNDADEDCFAVYGLRATTDVHQVGDELGNSFVW
                     VDGEKTDEELDGICTMGIQNADEAGLLNAIKNLGRDACKKFDVEFKGFQSYCGQNFIL
                     VKGDSARGGEDKGESIIRNPIVVAVFNS"
     gene            complement(13747..14094)
                     /locus_tag="PXA33_000960"
     CDS             complement(13747..14094)
                     /locus_tag="PXA33_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5120305.1"
                     /translation="MKELLLTTLCLLSLPAIAMTEKAEQETANALVSGDYQQLRNVAY
                     GMETGSFGHDHNPIAACALRRVILLVNSDKVDMTDFNNEAIACRKIEVTDNQQAWETA
                     FTIAKSISATKKK"
     gene            complement(14183..14608)
                     /locus_tag="PXA33_000961"
     CDS             complement(14183..14608)
                     /locus_tag="PXA33_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein GemA"
                     /protein_id="HDL5120306.1"
                     /translation="MQPQTRKQMIQKIHIGKNELKLDDNAYKMLLLEAVDKPSCSMMT
                     DSELMTVLQTMKAKGFVVKSKKYGKRPTASKTAPHRQGLINKIGALLYQSQKPWDYAH
                     AIAKRSFGIERVQWLTDDQLHKLVQILASYNHRHGMSVK"
     gene            complement(14592..15146)
                     /locus_tag="PXA33_000962"
     CDS             complement(14592..15146)
                     /locus_tag="PXA33_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5120307.1"
                     /translation="MKLCKCPICHSDISFEALIEDDAGRELLNTIVNIGGNCGRAVVA
                     YLGLFKPQKNSLANSRALKIVQSLLELYPPSNVLEKALMDTVEQVRRNRRETGRCEPL
                     ANHNYLKKVYESVKPQFAVVRSEAKAVSSLEQQQRQADQKKHDAVMYVERMKQMGQLE
                     AVKNTESYQIWLKWQEEKQNATAN"
     gene            complement(15146..15316)
                     /locus_tag="PXA33_000963"
     CDS             complement(15146..15316)
                     /locus_tag="PXA33_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5120308.1"
                     /translation="MEYQKIPEYRLCKKKSGEIVLQQLVICELTPATWHNPYPDSRTY
                     WVDLETIEEGTE"
     gene            complement(15387..15962)
                     /locus_tag="PXA33_000964"
     CDS             complement(15387..15962)
                     /locus_tag="PXA33_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5420 family protein"
                     /protein_id="HDL5120309.1"
                     /translation="MKFRFFKGDITQEPLKSIYADYQVSKEERKDKLNAILEKYPFND
                     GLISRSGWFHRIVIGIACKATNISQVLDVKGFKVGKFNDEFYLVKPDKRYEKGKELAE
                     DFKAINEIYEKHRDFSPYILKRLNMLYFVSDFSYSPTSGRSYLAVAGICENTLLVEMP
                     MPIDKNDEPFPEIAEGLVEIKESEFLAIQGK"
     gene            complement(15985..16167)
                     /locus_tag="PXA33_000965"
     CDS             complement(15985..16167)
                     /locus_tag="PXA33_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005822929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5120310.1"
                     /translation="MTEQEKNELNSQLNEALMQIIQAQKYLKQSDFIRSGVYLGTVQD
                     LLPKVHLKLLTANRKH"
     gene            complement(16234..16380)
                     /locus_tag="PXA33_000966"
     CDS             complement(16234..16380)
                     /locus_tag="PXA33_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HDL5120311.1"
                     /translation="MKTEQKATEFDRYKHYAEKAAEAERKGNYAEAQDHWKVAKLSAK
                     KTAN"
BASE COUNT         4342 a         4073 c         3086 g         5015 t
ORIGIN      
        1 ccgcaccaag ccctgccgca atcattgcat ttgattcggt tgctccctta taccagccta
       61 caataattcg ctcactgtta aggcttgcgg ttaaggttgt gccggtggca taagtgccac
      121 gccagcccat tacaccaata gccggtttat cttcaatcgg ggcagaaacc gattctaaat
      181 ggctacgcaa ggcaagggca ttttcatcat ctgaaaacgg cgaaataatc acgttataat
      241 gcgtaccggc aacactcgcc aacgccggag caattagggc attacgctga ccgttatcaa
      301 acgcacgagc ctcaagggtt aaacctgttg ctgtattgcg tgttgcaagc gtgatttcat
      361 tgctgatttc gccttttgct tttgaggtca gtttaagtgt cgtttcactt tccacacttg
      421 ccaccacagg gctataactc gccgcattaa tcacattaac taaacgagcc acaatcgcct
      481 gtgcttcttc gccttttgcg gctgcaactt cgtaagctgt gccggcaatc gtaatacgca
      541 ccacaccggc ataagttact gtgccggtaa aactaacttg ccctgttgct gcaatacctg
      601 cctcgtggtc ttttaagccc accaccgtta aacggattag cggattattt tggattgctt
      661 ggcgaaccat taaatgagct accgagcctt taccaaataa attttccgcc tccacatcgc
      721 taaaaatctt ttgcggagca gtaaaagcag cctcaccgtt tagcattggt gcaacaatta
      781 gcacctcttg ctcattggtc ggcaaggtgg ttactgcatc tttgttgttg tattcggtca
      841 gcacacttgg tttgcgaata ctgttcggga ttttctcaaa ttcaattttt gacattattt
      901 gccccctgtt tctttagcgg atttggtttc tttgacgaca atcaaatcgc catccgcaat
      961 gcggcgttga tagtaaaggg tgttttcaac ctctactgcc tcttgttcaa tatagtgatg
     1021 cggtcggttt tctaccggca cacgcacccc tttagcggct ttcactttta acatctaatc
     1081 accatcctta aattctgttc taaatgccgt ttcggcattg ttattcgggt caaaaatccg
     1141 attattaaag cccgataaat cgccttgcgg ttcatctagc ttgccttgat actgcttaaa
     1201 gacttgctca ttcggctcaa ccccttcggg gaatctacca tcatcaagca cattgcactc
     1261 gttgtagctg atttcaaact caatagcata agcagagagt ttctctttct tcacttccgc
     1321 attattccaa atcgtgcgga ttcgcttagg ctggataggg tggactaaac cgtctaaagt
     1381 ctggttaatc aataaggttt tcaccgctcc cagcaactga ttaaccccca cctcacgact
     1441 tgttacccca cctaaacgcc cagcagtctg atttcgcatt gagcgagtaa taaccaacac
     1501 cacaaacaca tcagtggctt gatagcgttt acctcttact cccattgatt tcgcctcgaa
     1561 cgccgagcca ccataagaga caagacaagc aggcaaacgg agaacatcta acttctcatc
     1621 gttaatttcc ccggcatacc cagcaacggc atacaccatt ttgcctaagc ccagcgttaa
     1681 acgctcaatc aaagcacgct caattttgct aatcatcgct tatcacgccc ccaaatacgg
     1741 ttgcccccgt taaaaaactg cactgtattt tcgccagtag ccgattcttc ctcattggtt
     1801 tcatcaaccg ctaacgacac tacgccctta gagatgtttt ccagctcttt taagcaaagt
     1861 ttgtagcggt tttccacctc ttcggtcatc gttactaatg atttgctggt taaatgataa
     1921 cgagccaaat cacagcaaat acgctttaaa ttttgcggta cagtctttaa tggcagacgg
     1981 taacgcccac ttaaatagcc atcaatttgg ctggaagagt ccgctaaagc tacttcaaga
     2041 gtcgcttcat caaccacgcc taccatttca cggtcggtta gctcaatgga ttcctgttcg
     2101 ccaattcgca aaacgaaatc atcaaccaac gcatacatta cgcatcgccc tcaacaacag
     2161 gtacaaactc caaataaggg tcgttggcta aggtaataat ttgttcatca ctcaaggctt
     2221 tacgagcaat ttctaccgcc gtttctttat taaaacggta gccacaacgc ccataggtgg
     2281 cttgtgggtg gatagatttg agcttaactt caaaagcaat aggatttatc aaagcaccgt
     2341 catcaggctt agtttcactt tcaggcggtt taggttgttc atccgtttta tcttcaggct
     2401 gttcattcac cttatcttca agcggtgtgt tttcgtttgt gttcaaagct tcattgtcat
     2461 ttttctttgg ttttgccatt ttgctaccct ctcaaaatgg gcgtattgct acgcccaaca
     2521 ttaaacttat tcagcaataa atggattcac taccactttt aacacacctt ttaaggtgtt
     2581 ggttgtgccg tcaattaatt ctgcttcgag taatttgcgt gcagcaaact caagcgaagg
     2641 tggcacaagc aaagtggtcg ggcgaatatt taactgctta tcgccatcgc cttttaaggt
     2701 gcgcattgca gttaatactt tttcaagggt ttcactattt aactcactat cttcaacacg
     2761 gtgaattaac tgccagaaac caaaaccggc attaccacga gcacgcacac cccataaata
     2821 ttggtctttc ataaagacgt ggtctgactc acttggatca aacttcgttt caatttcagg
     2881 tgtagtacgt tcttgccaaa ttaacggctt aatggcttgg ctatcatcta gcacatagaa
     2941 cgcaggctta ccgctggcag agcctgtggt gatattactt tgtgttttac ttgtgccagt
     3001 tccatcaaca ttctgataaa cagggtgatc ggtatcaaag aagttctgcc cgtcatagca
     3061 aagggttgtc ttaccttttt tcaacaaact aaataccaaa tcatcaggta attcagccgc
     3121 actttgtgcc gcttgtttca ccattggggt aaataagccc acttggtcat cttcaatggc
     3181 agtacgaggc acagcaacgg ttgattcaaa taatttattc tcaatcgtca ttccttgtgc
     3241 ttgcatattt ttaatttgac ggtcgcccac ccactcttgc attttcggga attgtcccag
     3301 ccaagcatag gtattagtag cggtgctgga gcttaccttc attgcaaccg ttccccattg
     3361 tggttcaagc acttttaaac cagcactaaa ttctttacga aatgcagtat caagtgcttt
     3421 taataattca gattttttaa acattacttg gattcctctg ttttatattt ttcaatgtaa
     3481 gctgcttcgc ttaaaccaag cgattttgct gctgcaattt ccgcagcaga taacgccact
     3541 ttctccgtac ctttgttcgg gtcttcaccc cccgactgcg tacttgctaa agcggggtta
     3601 ggtgttaccg ttttcaaaaa gtcggataaa gccactaaat cttgcttacc gagcttttcc
     3661 gcccacgctt tttgagccgg caataaccga ccgtcagaaa gagcggtttg aattaacgca
     3721 tcacgctctt tatcgttgat ttgctgtttt aagccgttca gctcggtttg cactgcttgc
     3781 aagtcgctta acgccacaaa cttcgccggg tcagggtgat tcactttggc agagagtgca
     3841 accacttggc ttttctcttt tgccagctcg gtataaacat cacttaacgc caccgggctg
     3901 tcgcctttgg ctttctcaag tgcggtcagt ttttcggtaa tctgctcatc ggtcgcattt
     3961 gccgacaatg caaataattt aattaagagt tctttcattt ttggattttc ctcttcgggg
     4021 gttaaaaatt tggcaaattg gctagacatc gcaaccacct cttgcagatt atgcaaggca
     4081 gggcggttag ttaaggcggc attaagtacc ttaattacca ttccgctgcc gtctgtcaaa
     4141 aacagtggcg agagatagcg ataaacgccg tctttaatct cttgggtcgc ttgtggtgtc
     4201 cacttaacat ccgcaaacaa gccttcgccc gagatatatt ccgccttggt aatccaaccc
     4261 gcagcagggt tgcctttgcc gttctcggcg atgtacaggg tctgatgctc ataatcaatc
     4321 atcagatcaa tcgcagtgtt gttaatatca tcggctaacg catagccatt ctcgtcacct
     4381 acataccaac gtcctccacg cccgtcttga gcctcaaacc aaccaaaagg gaaaagttga
     4441 atacgtccat ttgcctcttg gttgagttta aaactcaatg caatggggtt tagtttgaat
     4501 ttatgcttaa acacaaaaac acctcaaaac aaattaatga ggctaaagaa tagaagattg
     4561 agaaaaaaga gtaacgggga gcaacttcca caagttgcaa attttcagaa aaaaacaacc
     4621 gcttgtattc agcgaggaat gtaacccatt ttaaaacgct ttaaatccgt tttaaaaaat
     4681 tttaaaaaag ttggaatgat aaatcaccat aaaaaaacaa aaacgccaca gagagcgatt
     4741 taggggctat ctgtggcgat tatcttatcg cttaatccag cagtttttgc cagtaggctt
     4801 gcacatcgtc caaaatatct tgcctgtctt gcgaggttaa ttgtaaaaaa ggtcgggctt
     4861 caatttttac tttacgccca cgcccagcca taccaccgaa ttgatgaata gcagcataag
     4921 gctcatttgt gccaacaatc gccgaatcat tattataatc agcagtaatg ctgcccatta
     4981 aattttccgt atcaactaac ggcgtgcctt gccgatgttt tatacccagc catttcgggc
     5041 gaccgccctc atcaaaattg gtcagtaccg ccgattccat tttgccggca atattccgca
     5101 tcaagtctgt tttatttgcc gttttagcag caatacggtt aagcgtggca ataatttcat
     5161 caatgccgtg gatttggatt tcaatcattt ttaaccttgc aagttgattt ttaaaataaa
     5221 caggggtata ttccttttac ggttattaga aaagcggtga taatctccct gccgcagcct
     5281 atttgagatg gcaagtcctg ctcaaatgac cgagagcgtg tgtaagtgac cgggagcctt
     5341 acctaataac cgtatttttc aacagcagct tatccaattc tttttgtcca atctcccttg
     5401 ttgataccag aaataattca ccaaactctt ctaagtattt caccacaaca agaatacgat
     5461 aatgctcaaa gtttcgtata aagctgaaat ttttgccttc ttgcaaaata tgattaggct
     5521 gaaaaattac atcaggaata tttgcataca catcaaaccc aaaatcctgc ccctcacggc
     5581 tataaaactg cttaatcagt gtgtcatccg aaagccatac cgtgccgact ttactgtcga
     5641 ttaagcgttg tgtttcctca cttaaccgac cagcagcaaa tttaaagttt tgattaaggg
     5701 catttcgcac tttaaccatc tcatcaggcg agagtttttt cgccccatct ttggcaagtg
     5761 ccacagcctc ttctaacttg ttaaacgcct gcttaaattc accaccgctc atttctgctt
     5821 tggtaaattg gtaagccagc ttttcagggt ataaatccaa attcggctta taggttaaac
     5881 gccccacatt ataatcaaaa cctttatcag ccaatcgcac cgtaccatca gccaatttaa
     5941 agccaatggt ttcctcttgg ttgcctaact tatctttcgg tcgttttgcc ttcactaaaa
     6001 acggctcgct actgccgact tcgtcaatcc ctttacgctt taaatcccgc tcacttaacg
     6061 caatcacggt acaacggcaa ttaaagccgt tcggcggata aaaagtcgcc caaaacggat
     6121 cgtcataacg ataaatctta ccctgtaatg cttggtgagc agggcgggtg cgttggtcgc
     6181 ctaccgccga atattgccaa taagggcgat tatccacatt atccatatac cgctgataac
     6241 gagccgcaga ataagcctgt tgcatattgg tacgataaat cgtattcaag cggcgaggcg
     6301 tgccgaaatg ctcgccggtt ttcgggtcgg caagcagttt gccatcaatg ccacgactaa
     6361 tcgtcgtgtc catacaccca gcctttccgt tcaaattctg ccgtaagcgt tttcttccat
     6421 tcattaaagg atttcccctc acgccgtgct ttttccatcg actcataaat atcacgggtc
     6481 atttcaaggc tagtgagctt gctaatggtg gcggctcgcc ccaatgcaga atcaaataat
     6541 tcctgcttaa acactttatt cgcaagcaac ttcttctgcc gtaaaaactc aatcgcctgc
     6601 ttaggctcta gtccaatggc aaaattaact ttctgcattc gcagccccta ataaatccgc
     6661 caaaaataac gcattcgcta aatagcggtc gtgttcttca ctcactaaat cagggtaggc
     6721 tgccgccaat ttttctgctg cctcatcata agagctacac gccattacca ccgccaccgc
     6781 ctttttcgtg atggggtcaa attgggcgtt aaaatccggc acggaaaaag cctcgtctgc
     6841 caaatcatct aaggcatctt gctctttaat gcccgttttg gtttgggccg acaaagcgtg
     6901 aaccttgccc ccacaaccgc attcgcaagc ggtcattttt tgcggaaaat ttgcggataa
     6961 ccccaccgct tgtggcgggg cagaacgccc taacaccgct tcattttctt gagcttcggg
     7021 aataccgagt ttatccatcg cccactgcac cggaatcggc attccaatat ccaccaattt
     7081 cggcaagctc tcggcaaata acgccaagtc ggcaggctct ttggtatcaa actcaaaact
     7141 cggaatgcgg gttgggtcga tattgccaaa attaatttgc agaaacggca gaataatttg
     7201 ttgggtaaac gtctgcccca gctgtttcac gtccgacacc aacaaatcac gccgcacttc
     7261 gttatgcaca ttgccgagtg cattggtaga gcttttgccg tctgcaccgc tggttaaggt
     7321 ttgccccaaa atcaagcggg cgatagactt ctcacaccaa tccaccattt gcaaaaacgg
     7381 attattgccg gctgcaccgc ctgcattagc ggcgttatgc aactcaatac tcatcgactc
     7441 cggcataatt ccggcagcgt tgtgtccgat ttccgctaat gcacgtttaa gcgtttgttt
     7501 ttcgtttggt gttgcccctg caccgtattt accaatgcgg ataggcatac catacagctc
     7561 caaaaactcg gcaaagtcgt ggatagagta gtgtttaaac atatacagcc aagccagcga
     7621 gcggaataaa ttattgcgtg ccgcctgtgt ggagcgggat ttatgcgtat gcaccaccca
     7681 gccaaattct cgtaatggct caccttcggt attttgtcgg gttttcagca acagattgtc
     7741 gtgcttatcc cacttaaacc acgattgagg acggtggata aaggcgttcg gataccacaa
     7801 tttaccgtta aacgcccatt caatttcgag ggcagcaaaa ccgtgtccca cagcgtccat
     7861 acagtccatc accaaatctt caaggttgcc gagctgataa aataactcat caacctcatc
     7921 acgcagtttt tcttcctgcg gtgtggcatt gcgaggggca acaatcgccc aatctacgcc
     7981 taatgcggca cgcttacggg tttggatatt ggcaaaaata gagctgtccc gctcctcaat
     8041 atccataaat aattcgtgtt gagcggtaat atcgccattc tccgtatctt caaaaatcgc
     8101 tttcatttta gcaggcgtaa taaaattgct cggatggtcg gagagtaccc gacctgtagc
     8161 ggtaatttcg gctaaatccg tctgcaattc ttgattttta atcgggttta aattcggttt
     8221 aatctgcttg ttatgcttct ttttgctcat aaattttcct tttcagagct tagttacgct
     8281 caatatagtc cccctcttta gcaaagaggg gagttacatt aatgccgcca cttactgcga
     8341 tactcaccgt caaaatcatc tacactctcc cactcaatcg gggcagagct gctgagtgca
     8401 tttcgccata acatttccaa cgcatccgga ccatcatcgt gatcggcttt cggaaaatgg
     8461 cgaagctggc tttcaagcgt agattgcgag cggtgcaaca aaatcaaacc gttggcaata
     8521 tgcggttgca agctctcaat ccgtaacatc ttgtcactgt tcggcttggt tgccgttgcc
     8581 ggcaccggct taccacgctg ggcagaacgc ttgaccagtt ccgtctgcaa aaactcctga
     8641 aattgcaccg tctccacaaa ccagcggtga cagttatatt gcgaatgcag tctaatcacg
     8701 tcctcaataa ttaaatccgg caatcgtttt ttgatttgtg cctcaacaac atagagcttg
     8761 ccactctcac gatgatagcc accgaccaaa attgcagatg ggtcacggct tgccccagct
     8821 ttccccattg aggggtctaa cgccccgaaa taaatcaggt catcaggcag ttccgtccaa
     8881 tactggatac tgttggcaaa aatcgcatca tcacccgaga cagggtcatt ttgatactcg
     8941 gagtcaaatg ctgagtgccc atcactagca cggattttca tcaagtagag gatagggcga
     9001 gctaaccaag agactaacgc tcctttatcc atttctgctt tatgctgttg ataaaagaga
     9061 tcagacatag tgtcatcgtc cccttcttcc gagaggtata tattttccca ttcgtcccat
     9121 aacgccatat tgccgggaaa gcgtaaaatg gctttaaaac gaactcgccg ccaacctttt
     9181 gtatttaaaa tacggctaag cacactgtca taatgtagaa ttgtgccgac atagataaca
     9241 tcaaactttt cacctgcagc accaagtttt aaaactgctt ttaaaaccca gttatgcaat
     9301 ttattacgct gtgcaggtgt ttctacgctt tcatcattct caatatcatc aagtactact
     9361 aaatccggac gataagctcc gtggcgacga ccacgcaatt tttgcccagc ccccacagct
     9421 tcaacttttt gttttttagc cgtcataatg acacctgcac gccaaacctt gccttgtgca
     9481 acctcgggga aatcaattct taaacgaggg ttagattcaa tttccacttt aatagcttca
     9541 agcatactgt atgcctgttc tttggtgtcc atcgcaataa tgatgtatct ttttaaatca
     9601 cggactaaac accacagcgg gaataattgc gtacaaatcg ttgattttgc ctcgccacga
     9661 ggggctgcaa tcgcttggcg gactgactta gaaagatcac ttaccgaaag cggtaaattc
     9721 tcaaagagat aatgatgcaa ctgtgattta tgttcagacc gcacatagtg tggaaaataa
     9781 gcctgcacaa aatactcaaa gccgtgaaca gggtctaaaa ccttttgacg gcgttcgcta
     9841 atggcttgtg gtttatcgtc ccaaccctca aaactcgctt cgatattttg ttgtaactgt
     9901 cgccgtaatt cttccagttc tttttcaaat tctttgattt tcataaatta catacaagct
     9961 gcgataagca acaaccagcc ccaaccgtct gtaccgttga gcattagttt tattgctccg
    10021 aaaatacaag caagttggac aatccaacgg aaagagtaat gcttatgcac tacagtttgt
    10081 tttttcttag ccattactta aactcctttt tgaattgcac ttctagctca tctaacattt
    10141 ctaaaaagtc aggcaataaa tgcggtttct tggttttgac gatattcgcc atcatctcaa
    10201 tcgcacgcat agcggttgcc attgcacttg tttcaggcaa tagtttcttg ctcgatgccg
    10261 tcatcttcgc aaacgaatca gccagagcag ctaatgcctc aaccttagct gcggttggca
    10321 tttcggtgtt ttcctcaagc tccgtcatca acgtgcggta cttaattaaa aaaccggcta
    10381 acaagccttg agcgatattc tcaataccac cgcttgccat cgcttgcaca tcacgagctt
    10441 tttcccagtt atcaccgttc ttttcagcag ccgctttcca acgccgtgcc gtgccgaatg
    10501 atacccctgc acgttctgct gccatttcga ggcttaaacg gtcaaacaca taggcttgcc
    10561 gaaccgcttt ttgtacttca acatcgtgtg ccatacgccc tctacattcc aaatttcagg
    10621 cgtaacaact caaaacccac agcaacaatg ccaccaccca agccaccggc aactacagca
    10681 tcacggcggt ttttacgtgc catttcattc actaaacgat tagtcgcttg gatttcacgg
    10741 tgtaagcgtt cgatttcttc gttttgttta tccaccttat cgtttagctc atttaatcca
    10801 cctaaaatca ggtctaattt ttccgaatcg gtttgctttt gttttttacg gctcattact
    10861 tatccgcctt tttatcaagt ttttggttta cttctttgag cgagtctaaa atatcgtcca
    10921 gcttttcctt aatccctcta ttcacttcgt gggcaagctc tttggattga tatttgctct
    10981 ccatttctgc ttttagctct tttaccgttt gctcattgcg gttgatacga tcaaatatga
    11041 ccttgcctac aaacgccact aacggagcaa ctacaaaaga gatcagcatc tgaaacagtt
    11101 tttcatcaat cataacgccc ccttttggct cgcccgaccg ggtgttgatt accttgtgcc
    11161 accgccgcac gcaccacgtt atgcaattct gcaaggtcag gacggcggcg ataaaaacgc
    11221 cattgttgcc aacgctgtct gagtttttta attaacttca ttctggcaaa cttcctcata
    11281 ggtcagattg tgtgcaagca cttggcgttt cgtttccatt gtgtccgcac ggctcgggta
    11341 aatcagaccg aacgccgtgc aacccgttgt cgtcacggaa gtaaccgtgt tggtgcaact
    11401 gctcatcaat ggcactaggc tgaatacggc gagtttcact aacgtgtttt tgctgaattt
    11461 tggcattttt aacctctgca tttttttgtt gaatttcgac cgcttgttgc tgcttttccg
    11521 cctttaatgt agcggtaagt ttccgctcct gctttaattg ccagctttta taaaaggcat
    11581 aggctaaaat tgtgcttact acaccaacca cacctgcaat ttgttgagta atcattcttc
    11641 agcctccctt gagcgttctt tttgttttaa cgcacttacc accccttttg tggctgggct
    11701 acccaataca caaataccag cataagcaaa ccaccagtcg gaaataaacg gcttatcgta
    11761 aaggtaggcg tgaattaaaa tgcccgataa cactaaccag ctcataaact gaataaagcc
    11821 agtggtgctg gctgtgccgt ctttattggt aattaaatca agtaaaaatt gcttcacgat
    11881 tattcctcca ccgtgtcttt ggcaaaataa cgcaaattac ccgccattcg gttaatccaa
    11941 cctctaccaa aacggtcaaa gtgtttgagt ttggtgtaaa actcaaggcg ttctgcctga
    12001 aataacaagc ccacatccgc aaccgaataa cgctcaatcg cagccaaagt cactttaccg
    12061 ataatgccgt catcagccac acctaccgct cgctgtaaca ttcggctagc attgcccgaa
    12121 ccgtggttta cacacgcatc taaaaactga aaagccaact caggcgggaa ctgctcacag
    12181 ttataacgtt gccaaaaggc tttgaggtaa atagttttcg catccgcacg gctcattgca
    12241 cgcatagagc cggtatagcc attcgcacgg gcagtatgga tagtgatacc ccaattcgtt
    12301 tcgccaccgg ggtctttcgg gtcgttcaca taaccgccct cgtgtccgat aagacggtca
    12361 aatgcaagat taaagtcagc aatagttgcc ttgtttttga taggttcagt cataaaaaaa
    12421 gcctctttaa attcaatgat gatttaaaga gactttaatg atttagagca acggggagca
    12481 ggcgaaagga ttccacactt aaaacaaggt agcttgcgaa acggtatgct tacgctgata
    12541 atatcgtact gcctcccacg caacacgatc acacacgctg tatttagggc aaatttgcat
    12601 aatcgcaagc ctgccactta gccctccttg ctcggttaat tgacaataat cggcataaat
    12661 ctgctgatta cgcaacattc ttaatgcggt ttcacaacgt ggcaaataga cctcgcaagc
    12721 tcccatataa gcctgtaatt tcactgcatc atcttgccct aatacctctt tgagcttatt
    12781 aaaatagagt tttccgttag aaaattggaa tgaaaagcca ccaaaggctt tgattagctt
    12841 ttcagttgcc ggcaaaccaa tcacgcccac aatttctttc accgtttccg gcaaataatg
    12901 ttcgacactt tcaaattcag acataaaaaa tccccttgaa taatttccac gaaagccatt
    12961 atccaagggg gttattcaaa tctgtaatac ttacttaaaa atttttttgc aaatttttta
    13021 aaagctcagc ccattttgtg tacggcatat ctgatctagc tgcggttgat gacagttctg
    13081 tctcccaccg cattactatt ctttgcccga cccctaatat gttagctacc tctgtttgtg
    13141 tgaggttatg gtgctttctt agcattttta agttgcttgg tgtgtatcca aactctgggt
    13201 tttcaatcat tcacctccct ccattaaact agctattaaa tacagcaaca acgatagggt
    13261 ttctaataat gctctcgcct ttatcttctc cgcctcttgc actatcgcct ttaactagga
    13321 taaagttttg cccacagtat gactggaaac ctttaaattc aacatcaaat tttttgcaag
    13381 catcacgacc tagattttta atagcattta acaaacctgc ttcatctgca ttttggattc
    13441 ccattgtaca aatgccgtct aattcttcat ctgttttttc accatcgacc caaacaaaag
    13501 aatttcctaa ttcatcgcca acttgatgca catctgtagt tgctcttaaa ccataaacag
    13561 cgaagcagtc ttcatcggca tcgtttagta aagcatttaa aagtttttgg taatctgtca
    13621 ttttaatttc tcctgtttgg ctatgggtgc attatagtaa tataattacc tattgcaagt
    13681 gatttttcgt ttttttctta aattttttta tgtaaataaa aaagaccgct tgaaagcggt
    13741 ctaaatttat tttttcttgg ttgcagaaat ggattttgca atggtaaagg cagtttccca
    13801 agcctgttga ttatctgtaa cctcaatttt acggcaagca atcgcctcgt tgttaaagtc
    13861 agtcatatca accttgtccg aattaacaag caaaataaca cggcgtaaag cacaagcagc
    13921 aataggattg tgatcgtgcc caaacgaacc agtttccatt ccgtaagcta catttcttaa
    13981 ctgttgataa tcaccactta caagagcatt tgctgtttct tgttcagctt tttctgtcat
    14041 tgctattgct ggtaacgaca ataagcataa ggttgttaat agcagttctt tcattttaaa
    14101 tacctttaaa agttgattaa aaaacaccta aaatctacca caaaaaagcc cactatttaa
    14161 agtgggctat gcaataaatt tattatttaa cactcattcc gtgcctatgg ttataactcg
    14221 ccaatatctg cactaattta tgcaactgat catctgtcag ccattgcacc cgctcaatac
    14281 caaacgagcg tttggcaatg gcgtgggcgt aatcccacgg cttttggctt tgatacagca
    14341 acgccccgat tttatttatc aacccttgcc ggtgaggggc ggttttactt gctgttgggc
    14401 gtttaccgta ttttttcgac ttcaccacaa accctttagc tttcattgtt tgcagtacgg
    14461 tcattaattc gctatcggtc atcattgagc agcttggttt atccaccgcc tctaacaaca
    14521 gcattttata ggcgttatca tccagtttta gctcattttt gccgatatgg attttctgta
    14581 tcatctgctt tctagtttgc ggttgcattt tgcttttcct cctgccattt cagccaaatt
    14641 tgataacttt cggtgttctt aacggcttcc agttgcccca tttgcttcat tcgttccaca
    14701 tacatcacgg catcgtgttt cttttggtct gcttgccgtt gctgttgctc aaggctactc
    14761 actgctttcg cttcacttct tactactgca aattgtggct tcacgctttc atatactttc
    14821 ttcaagtaat tatgatttgc cagcggctca catctgccag tttcacgccg attccgtctt
    14881 acttgttcca ccgtatccat taaggctttt tctaacacat tagagggagg gtaaagctcc
    14941 aataaactct gcacaatttt caaggctcgg ctattggcaa ggctgttttt ctgtggctta
    15001 aataacccta aatacgccac caccgcacgc ccacaattcc caccgatatt gactatcgta
    15061 tttaacaact cacgccctgc atcatcttca atcaacgcct caaacgagat gtcggaatgg
    15121 caaatcgggc atttgcatag tttcatcatt ctgttccttc ttctattgtt tcgagatcaa
    15181 cccaataagt tcgactatca gggtatgggt tatgccaagt tgctggagtt agctcgcaaa
    15241 taactaattg ttgtaacaca atctctcccg atttcttttt acataaccga tattcaggaa
    15301 ttttttgata ttccatttta tgctcctata aaacacatta ttcagcccac ttaaataggg
    15361 gttaaatggg ctgtaaatgg gttttattac ttcccctgta ttgctaaaaa ctcactttct
    15421 ttaatctcaa ccaatccctc tgcaatctca ggaaatggtt catcattttt atcaataggc
    15481 attggcattt caaccagtaa ggtgttttca caaatgcctg ctacggctaa ataacttcta
    15541 cctgatgttg gggaataact aaaatcactt acaaaataaa gcatattcaa acgttttaag
    15601 atatagggcg aaaaatcacg atgtttctca taaatttcat taattgcttt aaaatcttct
    15661 gcaagttctt tccctttttc gtaacgttta tcaggtttga cgaggtaaaa ctcatcatta
    15721 aatttaccga ccttgaaccc tttaacatct aaaacttggc taatattggt tgctttacaa
    15781 gcaatcccta taactattct atgaaaccag cctgacctac taattaatcc atcattgaaa
    15841 ggatattttt ctaaaatagc attgagctta tccttgcgtt cttctttaga tacttgataa
    15901 tctgcataaa tgctttttag tggctcttgg gttatatccc ctttaaaaaa tctaaatttc
    15961 attcttaact ccttactggg gtggttaatg tttccgattt gccgttaata acttcaaatg
    16021 cacctttggc aacaaatcct gcaccgtgcc gagataaaca ccactgcgaa taaagtcact
    16081 ctgttttaaa tatttttgtg cctgaataat ctgcattaac gcctcgttta attggctgtt
    16141 aagttcgttt ttctcttgtt cggtcatcat ctcctcctaa aacggtttct tatgcatacg
    16201 tttacaaaat accgctcgtt gccccgccca gtctcagtta gcggtctttt ttgctgataa
    16261 ttttgcaact ttccaatggt cttgggcttc tgcataattg cctttacgtt cggcttccgc
    16321 tgctttctcg gcataatgtt tgtagcggtc aaactcggtc gccttttgtt ctgttttcat
    16381 tttttcctcc tgtggttaaa acactttata aatgcccttc tatcttgtcc ccctctttga
    16441 aaaagagggg ctaggggaga tttttaaagg gcattgaaaa ggttttttaa ctaagacttt
    16501 ctttttaact gattta
//
LOCUS       DANLRK010000042        16401 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.042, whole genome shotgun sequence.
ACCESSION   DANLRK010000042 DANLRK010000000
VERSION     DANLRK010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 16401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16401
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.042"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(<1..519)
                     /locus_tag="PXA33_000967"
     CDS             complement(<1..519)
                     /locus_tag="PXA33_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDL5120312.1"
                     /translation="MLDSLKEVNQKLDKKADKVGGGTKEDALVTLDTLIASGTVKAET
                     AMSLLPTLQKGAVATGASSEDMAKIAISSMQQFGIKEEDIGRALDMAVAAGQAGSFEL
                     AYMASWLPQQMAAAKQAGLSSIEGFERLLIANQQARVTAGTSDEAGNNLVNLLGKITA
                     KETNERFKNIEYK"
     gene            618..869
                     /locus_tag="PXA33_000968"
     CDS             618..869
                     /locus_tag="PXA33_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL5120313.1"
                     /translation="MAPTISDGDQMLVDFSKKEARGDDKIYLVQNGESVWVKRVRKVW
                     DYVELISDNESYRPIRITADEAQNLQIIGQVVHIGHSLV"
     gene            complement(1065..1265)
                     /locus_tag="PXA33_000969"
     CDS             complement(1065..1265)
                     /locus_tag="PXA33_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HDL5120314.1"
                     /translation="MQYVLDLTGYACPLPLLMAKKAMNDLKKGDSLEILLNQQSSLTD
                     FELLAKEQGWLCELQAVENVGY"
     gene            complement(1324..1836)
                     /locus_tag="PXA33_000970"
     CDS             complement(1324..1836)
                     /locus_tag="PXA33_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017357669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HDL5120315.1"
                     /translation="MQQLEPQTFASWAEPIDMLYACHSKVKSFCKQLQILPDYLEKNG
                     VNQAVKNDVQQILNYFNISAPLHHEDEEDDFFPELIKVQPQAQAEVDELERQHVHLHK
                     NWDYLSAQLEDLLAGKRENVDRDLIERFVAGYDVHIAIEEPLFELGREHLAQDKLSAM
                     GKVMADRRKL"
     gene            complement(1849..3003)
                     /gene="tgt"
                     /locus_tag="PXA33_000971"
     CDS             complement(1849..3003)
                     /gene="tgt"
                     /locus_tag="PXA33_000971"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HDL5120316.1"
                     /translation="MKYELKTTSGNARRGRLTFNRPKGEFHVETPAFMPVGTYGTVKG
                     MTPEEVKATGAQILLGNTFHLWLRPGQEVMKMHGDLHDFMQWHGPILTDSGGFQVFSL
                     GKLRKIKEEGVTFQNPISGEKIFLSPEKSMEIQYDLGSDIVMIFDECTPYPATFDYAK
                     KSMEMSLRWAKRSRDRFDELENPNALFGIIQGGIYEELRKVSVEKLVEIGFDGYAVGG
                     LAVGEPKEDMHRILEYVCPQIPADKPRYLMGVGKPEDLVEGGRRGIDMFDCVMPTRNA
                     RNGHLFVTNGIVKIRNAKYRADTTPLDPECDCYTCKNYTKAYLYHLDKCGEILGARLN
                     TIHNLRYYQRLMAEIRQAIEEDRFDDFVVEFYARIGKEVPPLQSEVKEQK"
     gene            complement(3062..3202)
                     /locus_tag="PXA33_000972"
     CDS             complement(3062..3202)
                     /locus_tag="PXA33_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5120317.1"
                     /translation="MKKLLLCVGILVLAACSSTGVGGSIGAGGGSNGVGIGLGVGTDF
                     RF"
     gene            3296..3868
                     /locus_tag="PXA33_000973"
     CDS             3296..3868
                     /locus_tag="PXA33_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1439 domain-containing protein"
                     /protein_id="HDL5120318.1"
                     /translation="MKILRKFAAVCVVSMAMLSANANLLTISEQEINDYLATKLAEKI
                     PLKDKVGIPKLFELDYRLHSLTSQIGRTEEKKVAISGVVDGLLNAKGKKYAASIHLNM
                     DTTPYYDPQKGALFLKDVRLLSWSATPAKYQAELQMFLPVLADGLSSFLNSTPVYTLD
                     ENKTKEAMVKKFGKAIVVENGNIKLETTIF"
     gene            3970..4188
                     /gene="cspD"
                     /locus_tag="PXA33_000974"
     CDS             3970..4188
                     /gene="cspD"
                     /locus_tag="PXA33_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein CspD"
                     /protein_id="HDL5120319.1"
                     /translation="MDIGIVKWFNNVKGFGFITSDVCEGDIFAHFSEIQAEGYRSLKV
                     GQKVQFELVQGERGASASNITPISEKTL"
     gene            4278..5192
                     /locus_tag="PXA33_000975"
     CDS             4278..5192
                     /locus_tag="PXA33_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDL5120320.1"
                     /translation="MPSSYQLVLINLLYIFGIGLNIVLLRFLSLHFDPLNNNGIRFLV
                     GGFTLLFFILWRFRTSLSQLIKTPKLIAISLFVGLMMSANMYFWLKGVKITNAVTVSI
                     FGVLAMPFGVIIAAIFFQDERERIKSKAFWFGSILTIIGSLGFIWHGKTLSIGEGFIL
                     GSVFLFLSIIIRNIQNLVVKFARELNVLTLSCVTSLTTSLSSLLLSQQAGKFNELFTA
                     PNWLLFSLMVAGIYAIMIGMVLTFHIIQTQGLITYQVLELLMPVSTAIIAYILLDERI
                     SIVQMGFACIVILGSSIALKLVKYPKHY"
     gene            5311..6171
                     /gene="araC"
                     /locus_tag="PXA33_000976"
     CDS             5311..6171
                     /gene="araC"
                     /locus_tag="PXA33_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HDL5120321.1"
                     /translation="MYKNLSLLSPTYPFDLEFIAGLTQCEKGNYLDSIVDRQQGMHGY
                     MLQLTTFGRGSVFDGKHVFTAHRGQLLLFTPNAVQHYHRHPESQYWHYKWVYFQPNPK
                     WNKWLNWSDMEEKIGRITISDDRYFQEISQLFSKIELELKSSKFFKEDIATSLLEYLL
                     MKCISAEKIEVIPVIDQRILTVCDLILTNLAKNESLEAMAEKVFLSPSRLSHLFKQSL
                     GISLVQWRELQRISESKKLLYFSNISISNIAKSLGYEDSLYFSKIFKKHTGLSPSEFR
                     TLERRKDSEM"
     gene            6358..7332
                     /locus_tag="PXA33_000977"
     CDS             6358..7332
                     /locus_tag="PXA33_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDL5120322.1"
                     /translation="MKKPTFKHALFSSILALACYGSAFANDVVNISKIDGMPWFNRMA
                     EGVVQAGKDNNINAYQVGPSNTDAPQQVKLIEDLIAKKVSAISIVPNDANALEPVLKK
                     AKQNGIVVLTNESVGQPSADWDIEIIDNAQFAADYVEEVAKAMGGKGGYVIYVGGLTV
                     PQHNLWADLFVKYQKEHYPDMFEVTSRMPVAENINDARRTTIDLVKAHSDLKAVVSFG
                     SQGPIGAGLAVKEKRLKNKLNVFGMMIPSQAANLIKSGDITMGITYDPANAGYALAAV
                     AAKVLKGEKIEDGLEIPTVGKAKVDSEKKLLQFHNVLKVTKENIDKLY"
     gene            7387..8877
                     /locus_tag="PXA33_000978"
     CDS             7387..8877
                     /locus_tag="PXA33_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDL5120323.1"
                     /translation="MAFISMRHISKTFHGIKALNQVNLSLDYGEALCLAGQNGCGKST
                     LIKILSGVYQPDKGAEIQIGATQYTKLTPQESIDKGIQVIYQDLALFPNLTVAENIAI
                     NLHRKFGWVSQDHIHQVALTAIQSINANLDLNAILEDLPIAQQQLVAICRALAQNARL
                     LIMDEPTASLTAKEVKDLLDVVIKLKSKGISIIFVSHKLQEVMSVSDSVLVLKDGNMV
                     GQYPIREMNEKRLGFLMTGLEIDYKRLDLPDFLQRKTVLEVRNLTLPHQYQEINFSLR
                     EGEIVALTGLLGSGRTELCLSLFGITQPQSGDILLNGEKVIFNTNRDAIAKGIAYVSE
                     DRMHTGLIMAESIHHNIISTIFHKITDKLQIIQSSKAYASSQALIDSLKIKVSDSDLP
                     VNTLSGGNAQRVSIAKWLAIDPKIIILDAPTIGVDIANKEGIFQIIKSLAQQGIAVIF
                     VTDEVEETYYNSHRVIVMKKGRIVDEILPAYSSEKSIAEVIYENQQ"
     gene            8861..9820
                     /locus_tag="PXA33_000979"
     CDS             8861..9820
                     /locus_tag="PXA33_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL5120324.1"
                     /translation="MKISNNHILSSTLLILILLLTLFTRDFATIENLYDVVNNYAMLM
                     ILACGLFVVLLSGGIDISFPAITIISQYVMVTLIGKYDVGFAGVFVISSTIGILLGFI
                     NALFVNRLSVPSIIITISTLNIFYGGLLYFTKGVWLYEYPEWFTQEIVFFKQITAEGV
                     EFGLSFQALMAILAVILTGLITNKLTIGRKIYALGGNPDATSRIGFNLLFLHLFAYGY
                     MGFMAGLAGIVQSYTVQSVAPDSLLGYELTVLAAVVLGGASLSGGRGTLLGTVMGVML
                     LAIIQNGLNLMNVSSYWQTIITGIIIILSISATALSQRKKQGA"
     gene            9823..10773
                     /locus_tag="PXA33_000980"
     CDS             9823..10773
                     /locus_tag="PXA33_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL5120325.1"
                     /translation="MKSKLDPTIFYLLALFAVAVIAFSIFIPDIFWSVSNFQSMASQI
                     PVLGILTLAMALPMLTGGINLSIIAGMNACSLIIAYFITQYTGISWLFIAILLSLCCA
                     LAIGLLNGTLIAIIRVSPILATLGTMTLINGINILVTNGSTIANFPETMLSINASAFL
                     WIPLPILIFLFLAGLIWFFLEKTAMGKAVYFIGNNEKATYYSGINTKKVLIWVYVISS
                     ILCVFAAILMMSKLNSAKASYGDSYLLISILAAVLGGINPDGGKGKLLGIMLALVLLQ
                     VIESGLNMLGISSYITMILWGSLLLFFIFLQKNRLFRLTQ"
     gene            10786..12381
                     /locus_tag="PXA33_000981"
     CDS             10786..12381
                     /locus_tag="PXA33_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HDL5120326.1"
                     /translation="MQNEKQLIEQGNTVIGIELGSTRIKAVLISSDGTILATGGADWE
                     NRLIDGIWTYHQNEIWEKLQAAYMDLSKTVKEKYHTTIRTAKALGISAMMHGYLPFDK
                     HGNPLVAFRTWRNNITLTSSEKLTALFQYPIPQRWSIAHLHQALLNQEPHLPEIDYLT
                     TLSGYVHWQLTDHKVLGIGDASGMFPIDTELKSYNPTMLAQFDRAIADYRMPWKLEAI
                     LPQVRVAGEQAGKLTEKGAKLLDPTDNLQAGIPLCPPEGDAGTGMIATNCIKEKTGNI
                     SAGTSAFAMIVLEKALSKAYSELDIVTTPAGKLVAMAHANNCTSDINAWVNLFGECLS
                     VFGVNICTEELYEMLFLHALQGDADCGGLLSYGFHSGEHNVGLSEGCPVFLHPTNAHF
                     NLANFMRTHLYTAFGAMKLGMDILINQEKVEISRILGHGGIFKTEGVAQKILSSALNI
                     PLATASTASNGGAWGIALLADFLSVSDRYSLEEYLDNCIFNTTKLNLVQPDKTMSEGY
                     ERFMQRYKQGIPVVEKALFLNTD"
     gene            12611..13813
                     /gene="yedE"
                     /locus_tag="PXA33_000982"
                     /pseudo
     CDS             12611..13813
                     /gene="yedE"
                     /locus_tag="PXA33_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005824242.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
     gene            13849..14118
                     /gene="yedF"
                     /locus_tag="PXA33_000983"
     CDS             13849..14118
                     /gene="yedF"
                     /locus_tag="PXA33_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007241376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HDL5120327.1"
                     /translation="MAFTVVQKLDKDPKDITPDYTLDTLGEPCPYPAITMLETMPQLQ
                     KGEILELLSDCSQSINNIPIDTKNHGYTLLSVEQNGTKLRYLIQR"
     gene            14218..14562
                     /locus_tag="PXA33_000984"
     CDS             14218..14562
                     /locus_tag="PXA33_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDL5120328.1"
                     /translation="MNKYALLAIFWFIIGIYALLFRESTNDIPPFPHFDKVSHFALFF
                     IQIWLSANAFIRSKKGIPYFGLIIFALLYAFTSEWAQATFTSTREGSWLDGLADMLGA
                     SLSLLLVKYCKK"
     gene            complement(14625..15572)
                     /locus_tag="PXA33_000985"
     CDS             complement(14625..15572)
                     /locus_tag="PXA33_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HDL5120329.1"
                     /translation="MNRITQILNIKHPIVQAPMSWLTDAHLVASVAEAGGLGFLAPHA
                     GQTTNPTSNEEVLDRMRNEIRKVKALTDKPFGVPFILSYDFSLIPLMVDLFIEERVPV
                     VLDNGWLDPEIYAKFKAAGIKIICRLPNPNLADALKAQALGADILVLTGFDEGGTLPM
                     KEVGSFNVLAEFVGKVDLPIMLAGGIADKKAVQAALTLGAEGVWIGTAFIATEECRAS
                     EKVKNWIVEANANDLLLFRTEPHYYRSLPTGLAKKCFEMSENGATRSEIAKVMNAGTG
                     MRLGMLEGDFDNGYVSVGNGISQINKIKSVKELIDELMA"
     gene            15755..>16401
                     /locus_tag="PXA33_000986"
     CDS             15755..>16401
                     /locus_tag="PXA33_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5120330.1"
                     /translation="MSIRWENIKPLQGSQNSAFEELVCQLARQEFKSQGKFTRISAPD
                     GGIEAMCELPDGSVCGWQAKYFLSSFSSSQWQQIEDSFKESLKNYPKLTKYYVCVATD
                     RANANVSGKKSFLTKWEEYTQRWKDFAQSQGREIEFEFWGSFELSDLLSKPENAGKKF
                     FWFNESELSDKWFEQYNQQAINNLGVRYTPEINVDLPIAMQLESLARTQIFKKHL"
BASE COUNT         4810 a         3313 c         3182 g         5096 t
ORIGIN      
        1 tttatattca atatttttaa aacgctcatt tgtttcttta gctgtaatct taccaagcag
       61 atttaccaga ttattacctg cctcatcgct tgtaccggca gtgacacgag cctgttggtt
      121 tgcaattaaa agtcgctcaa agccctcaat actactcaaa ccggcttgtt ttgcagctgc
      181 catttgttga ggcaaccaac ttgccatata tgcaagctca aaactacctg cttgcccggc
      241 tgcaacagcc atatccaatg cccgcccaat atcctcttct ttgatgccaa attgttgcat
      301 agaggagatc gcaatttttg ccatatcctc cgagcttgcc cctgttgcca ccgccccttt
      361 ctgcaaagta ggtaatagac tcattgcggt ttcggctttg actgtaccgg aggcaatgag
      421 cgtatcaagc gtaactaacg catcctcttt tgtgccaccc ccaaccttat ccgccttttt
      481 atcaagtttt tggtttactt ctttgagcga gtctaaaata tcgtccagct tttccttaag
      541 agccttattc agacagccta ttaagcctag gcgtaaaagc ccataattgt ggcgtatttt
      601 gggctaatgg cacatcaatg gccccgacta tcagcgatgg tgatcaaatg ttagtggatt
      661 tcagcaaaaa agaagcccgt ggagacgata agatttatct tgtgcaaaat ggtgaaagcg
      721 tttgggtaaa acgtgtaaga aaagtgtggg actacgttga attgattagc gataatgagt
      781 cctaccgccc aatccgaatt acagcagacg aagctcaaaa cttacaaatc atcgggcagg
      841 tagtccacat cggacactct ttagtttaaa acagatttaa aatagtttta aaaccgatca
      901 aacctatgca aaaaatatcg caaaaactgc ccattttttt ctaatcctgc tcattttgtt
      961 ttttgcatag ccccaaccaa acaataacgg cgaacccttg tattcacggg gctcgccgtt
     1021 atttttccgc ccaaattttt ttctttctct ctatgcaaaa aagatcagta ccccacattt
     1081 tcaaccgctt gtagctcaca aagccaacct tgctctttgg caagcagttc aaaatcggta
     1141 aggctactct gctgattgag taaaatttcc aagctatcgc cctttttaag atcgttcatc
     1201 gctttttttg ccattaataa cggcaagggg caggcatagc cggttaaatc taaaacatat
     1261 tgcatagcga aatagggaat aaagaaaaag ggcgaatatt gaaattcgcc caatcacaaa
     1321 agattagagt ttacggcggt ctgccatcac tttgcccata gccgataatt tgtcttgagc
     1381 taaatgctct cgcccaagct caaagagtgg ctcttcaatc gcaatatgca catcataacc
     1441 tgccacaaaa cgctcaatta agtctctatc cacattttca cgtttacctg cgagcagatc
     1501 ttcaagctgt gccgacaaat agtcccaatt tttatgtaaa tgaacgtgct ggcgttccag
     1561 ttcgtctacc tctgcttgag cttgtggttg caccttaata agctcaggga agaaatcatc
     1621 ttcttcatct tcgtgatgta aaggggcaga aatgttgaag tagttcaaaa tctgctgcac
     1681 gtcattcttc actgcttggt ttaccccgtt tttctcaagg taatccggca aaatttgcaa
     1741 ctgtttacag aaacttttta ctttgctgtg gcaagcataa agcatatcaa tcggctctgc
     1801 ccagcttgca aaggtttgtg gttcaagttg ttgcatatac actccttgtt atttctgttc
     1861 ttttacttca gattgtaaag gtggcacttc tttgccaatt cgggcataaa attccactac
     1921 aaaatcatca aaacgatctt cttcgatggc ctggcgaatt tctgccatta ggcgttgata
     1981 gtagcgtaaa ttatgaatgg tgtttagtct tgcccctaaa atctcgccac atttgtctaa
     2041 gtgatataaa taggctttgg tgtagttttt gcaagtgtaa caatcacatt caggatcaag
     2101 cggtgtggtg tctgcacgat atttcgcatt acgaattttt acaatgccgt tggtcacaaa
     2161 taaatgtccg ttacgggcat tgcgggttgg cattacgcag tcaaacatat caataccacg
     2221 gcgaccccct tcgactaaat cttctggctt gcctacaccc attaaatagc gtggtttatc
     2281 tgccggaatt tgcgggcaaa cgtattctaa aatgcggtgc atatcttctt taggttcacc
     2341 taccgccaag ccaccgactg catagccatc aaagccgatt tcaactaatt tttccaccga
     2401 gactttgcga agttcttcat aaataccacc ttgaataatg ccaaataacg cattcgggtt
     2461 ttcaagttca tcaaagcgat cacgagaacg tttcgcccaa cgtagtgaca tttccatcga
     2521 tttttttgca taatcaaagg ttgccggata aggagtacat tcatcaaaaa tcattacaat
     2581 gtctgatcca agatcgtatt ggatctccat tgatttttcc ggcgaaagga aaattttctc
     2641 accgctaatc gggttttgga aagttacgcc ttcttcttta attttacgca gtttacctaa
     2701 gctaaaaact tggaaaccgc cggagtcggt taaaataggg ccgtgccatt gcataaaatc
     2761 gtgcaagtca ccgtgcatct tcatcacttc ttgccccggt cttaaccata aatggaaagt
     2821 gttgcctaat aaaatttgcg cccctgtcgc tttaacttct tcaggagtca ttccttttac
     2881 cgtgccataa gtgccaacag gcataaatgc cggtgtttca acgtgaaatt cgcctttcgg
     2941 gcggttaaaa gttaaacgcc cacgacgagc gttaccactt gttgttttta attcgtactt
     3001 cattttaatc cacaaataaa cagttcgtga aagcggtcaa aaaccaccaa aaatttacca
     3061 attagaatcg aaagtcggtg ccgaccccta agccgattcc aacgccgtta gagccaccac
     3121 cggctccaat actgccaccc acaccggttg atgagcaagc agctaacact aaaatgccaa
     3181 cgcataataa gagttttttc attttttgct gtcctaaacg aaaaagagag tctatgatag
     3241 acccaatgta ttctaacctg tttaatggat taaacctaat tttaaagggt aaattatgaa
     3301 aattttacgt aaattcgctg ctgtatgcgt tgtctcaatg gctatgctct ctgctaacgc
     3361 taatttgctc actattagcg agcaggaaat caacgactat ttagccacca aattagctga
     3421 aaaaattcct ttaaaagata aagtcggtat tcctaaatta tttgaattgg attatcgcct
     3481 gcattcactt accagccaaa ttggcagaac cgaggaaaaa aaagtagcta tttctggcgt
     3541 tgttgatgga cttttaaacg caaaaggcaa gaaatatgct gcctctatcc atttaaatat
     3601 ggataccacg ccttattatg atccacaaaa aggggcatta tttcttaaag atgtcagatt
     3661 gttaagttgg tctgcaacac cggctaaata tcaagcagag ctgcaaatgt ttttgccggt
     3721 gttggctgat ggattaagta gttttttaaa cagtacgcct gtttatactt tagatgaaaa
     3781 taaaacaaaa gaagcaatgg tgaaaaagtt cggcaaagcc attgtagtag aaaatggtaa
     3841 tatcaagcta gaaaccacta ttttttaaat aattttgtga tcttcgtcta atttttacac
     3901 tttgatagtt gagcttttac gcgttttatt tacaatccaa atatcgccat tcattatttg
     3961 gagagttcta tggatatcgg tattgttaaa tggtttaaca atgtaaaagg atttggtttt
     4021 attacatcgg atgtatgtga aggagacatt tttgctcatt tttctgaaat tcaagcagaa
     4081 ggttatcgct ctttaaaagt cggccaaaaa gtacagtttg aattagttca aggagagcga
     4141 ggtgcctcag cctcaaatat cacgcctata agcgaaaaaa cgttataatc cgtcgttaat
     4201 aaaattattg ttataaccat actttattag attttaaagc tcagctttta cagctgagcc
     4261 atttttgctt tttgaatatg ccttcctctt atcaattagt cttaattaac ctactttata
     4321 tcttcggtat tggtctaaat attgtactgc ttaggtttct cagtttgcat tttgacccat
     4381 taaataataa cggtatacgt tttcttgttg gtgggttcac cttattattt ttcattttat
     4441 ggagattccg cacatcgctg tctcagctga ttaaaactcc aaaattgatt gcgatttctc
     4501 tatttgtcgg gttaatgatg tccgcaaata tgtatttttg gctaaaaggg gtcaaaatta
     4561 caaatgctgt taccgtctct atttttgggg tattagctat gccatttggt gtgattattg
     4621 ccgccatatt ctttcaagat gaaagagaac gcattaaaag caaagctttc tggttcggct
     4681 caattttaac gatcatcggt tcactcggat ttatttggca cgggaaaaca ctctcgattg
     4741 gcgaaggatt tattcttggt tctgtgttct tatttttgtc gatcattatc cgtaatatac
     4801 aaaacctagt tgtaaaattt gctcgagaac tgaacgtatt aaccctaagt tgtgtaacat
     4861 ctttaactac ttcactctcg agtctattac ttagccaaca agcaggtaaa tttaatgagc
     4921 tttttactgc accaaactgg ctactattct ctctgatggt tgctggtatt tatgcgatta
     4981 tgatcggaat ggtattaacc tttcacatta tccaaaccca aggactgata acctaccaag
     5041 tattggaact attaatgccg gtttcaacag ccatcattgc ttatatttta ctggacgaac
     5101 gtatttccat tgttcaaatg ggctttgctt gtattgtgat tttaggctca agtatcgcct
     5161 taaagttagt aaaatatcct aagcactatt aggatctttg ctaattaata ttggtaaaat
     5221 tacatttggt ttactaatct gagcgttcat tcattagtaa tgataaacaa gcggttattt
     5281 tttgttattt tttggtaaat actgatagtc atgtataaaa acctttcttt gctttcaccg
     5341 acttatccat ttgaccttga attcatcgcc ggtcttactc aatgtgagaa aggtaactat
     5401 cttgattcta ttgtcgatag gcaacaagga atgcacggtt atatgcttca attaaccact
     5461 tttggtcgtg gttctgtttt tgatggtaag cacgttttta ccgctcatcg agggcagtta
     5521 ctcctcttta cacccaatgc cgttcaacac taccaccgtc acccggaaag ccaatattgg
     5581 cactacaaat gggtctattt tcagccgaat cctaaatgga ataaatggct gaattggtca
     5641 gatatggaag aaaaaatagg acgtatcacc attagcgatg atcgttactt tcaggaaatt
     5701 agccaacttt tcagtaaaat tgaattagaa cttaaatcca gtaaattttt taaagaagat
     5761 attgcaacaa gtttgttgga atacttactt atgaaatgta tttccgccga gaaaattgag
     5821 gttattcctg tcattgatca acgcattttg acagtttgtg atttaatctt aacgaatttg
     5881 gctaaaaatg agagcttaga agcaatggca gaaaaagtat ttttatcgcc atctcgcctc
     5941 tcacatcttt tcaagcaatc gctggggatc agcttagtgc aatggcggga attacaacgc
     6001 atttccgaat ccaaaaaatt gctttatttc tctaacatct ctatctctaa tattgcgaaa
     6061 tccttaggct atgaggattc tctctacttt tccaaaattt tcaaaaagca taccgggctg
     6121 tcaccctctg agtttagaac cttagaacgg cgtaaagaca gtgaaatgtg atcttgatct
     6181 cactttactt tgcatagcag aattttccat atcgataaca aaaagttacc tgtttttctc
     6241 tcattcaaaa gcataagata caaacagctt taatagtgct gtttctatta gagaagggat
     6301 atcaagctct gtgagtgata ttcagtgaaa taacttctta tgtatgaggg aagaacaatg
     6361 aaaaaaccaa cgttcaagca cgcattattt tcaagtattt tagctttagc ctgttacggc
     6421 tcggcatttg ccaatgatgt tgttaatatc tccaaaattg acggtatgcc gtggtttaat
     6481 cgtatggcag agggtgttgt gcaagcaggc aaagataaca atatcaatgc ttatcaagtt
     6541 ggtccatcaa ataccgatgc accacaacaa gtaaagttaa ttgaagattt aattgcgaaa
     6601 aaagttagtg ctatttccat cgtccctaac gatgcaaatg ccttagaacc ggtactgaaa
     6661 aaagcgaaac agaacggtat tgtggtatta accaatgagt cagtcggaca acctagtgcc
     6721 gattgggata ttgaaattat cgataatgca caatttgcag cagactatgt agaagaagta
     6781 gcaaaagcta tgggcggaaa aggcggctat gtgatttatg ttggtggttt aaccgtgcct
     6841 caacataatc tgtgggctga tttattcgtg aaataccaga aagagcatta tccggatatg
     6901 tttgaagtga ccagcagaat gcctgttgcc gaaaatatta atgatgcacg ccgtaccacc
     6961 attgatttag tgaaagccca ttccgatctt aaagcggtcg tttcttttgg ttcacaaggt
     7021 cctattggtg cagggttggc agtgaaagaa aaacgcttaa aaaataaatt aaatgtcttc
     7081 ggaatgatga ttccttccca agcagccaat ttaattaaaa gcggtgatat tacaatggga
     7141 attacctatg acccggccaa tgcaggttat gctttagctg ctgtcgctgc caaagtatta
     7201 aaaggcgaaa aaattgagga tggattagag atcccaacgg tcggtaaagc caaagtagat
     7261 agtgagaaaa aactacttca attccacaat gtgctgaaag tcaccaagga aaatatcgat
     7321 aaattatatt aatttgcttg aaatatgggc tgatagccag cccatatttt ccctcaggag
     7381 gcacttatgg catttatttc gatgcggcat atcagtaaaa cattccacgg cataaaagca
     7441 ttaaatcaag tcaatctctc attagattat ggcgaagcac tttgtcttgc cggtcaaaat
     7501 ggctgtggaa aatcaacctt gatcaaaatt ctttccggcg tttatcaacc ggataaaggg
     7561 gctgaaattc aaattggtgc aacccaatat accaagctga caccacagga atcgattgat
     7621 aaaggtattc aagtgattta tcaagattta gccctcttcc ccaatttaac cgtggcagaa
     7681 aatattgcca ttaacctgca ccgaaaattt ggctgggtga gtcaagatca cattcatcag
     7741 gttgccctca ctgctattca aagcattaat gcaaatctag acctgaatgc aatattagaa
     7801 gatttaccga tcgctcaaca acagttagtc gctatttgtc gagcgttagc acaaaatgcc
     7861 agattattga ttatggacga gccgactgcc tcacttactg caaaagaggt caaagatctg
     7921 ttagatgtcg tcatcaaact taaaagcaaa ggcattagca tcatctttgt cagtcataaa
     7981 ttgcaagaag taatgagtgt ttccgatagc gtattagtgc tgaaagatgg caatatggtt
     8041 gggcaatatc ctatccgtga aatgaatgaa aaacgccttg gtttcctaat gactgggctg
     8101 gaaatcgatt acaagcggtt agatttgccg gattttttgc aaagaaaaac cgtattggaa
     8161 gtccgcaatt taacgctgcc tcatcagtat caagagatta acttttcgct acgagaaggg
     8221 gaaattgttg cccttacagg attgctcggt tcagggcgta ctgagctttg cctaagttta
     8281 ttcggcatta ctcaaccaca atccggcgat attttattaa acggtgaaaa agtgatattt
     8341 aacactaacc gtgatgccat tgccaaaggg attgcttatg tatcggaaga tagaatgcac
     8401 accgggctga ttatggcaga gtcgattcat cacaatatta tttcgactat ttttcacaaa
     8461 atcaccgata aattacagat tattcaatcc tcgaaggctt atgcaagtag ccaagccttg
     8521 attgattcat taaagattaa agtatccgat tcagatttgc ctgttaatac cctttccgga
     8581 gggaacgcac agcgagtttc gattgcaaaa tggctggcaa tcgaccccaa aattattatt
     8641 ttagatgccc cgactattgg ggtcgatatc gccaataaag aagggatctt ccaaattatt
     8701 aaatcattgg ctcagcaagg cattgcagtt atttttgtga ctgatgaagt ggaagagacc
     8761 tactacaaca gccatcgggt aattgtaatg aaaaaaggtc gcattgttga tgaaatcttg
     8821 ccggcatatt ccagtgaaaa atcgattgcg gaggttattt atgaaaatca gcaataatca
     8881 tattctttcc agtacgttac tgattttaat cctattatta accctattta ctcgtgattt
     8941 tgcaactatt gagaaccttt atgatgttgt aaataattac gcaatgttaa tgattttagc
     9001 ttgtggttta tttgtggtat tgctgtctgg cggcattgat atttctttcc ctgctatcac
     9061 tattatttcc caatatgtta tggtcactct gatcggtaaa tatgatgtag gatttgccgg
     9121 cgtttttgtt atttcttcca ccatcggcat attattaggt tttattaatg ctctatttgt
     9181 taatcggctt agtgtccctt ccattattat tacgatctca acactcaata ttttttatgg
     9241 cggactactc tattttacca aaggtgtatg gctttatgag tatcccgaat ggtttaccca
     9301 agaaatcgtg ttctttaaac agataactgc tgagggagtg gaatttggat tatcgtttca
     9361 agcattaatg gctattttgg cggtgatatt aaccggctta attaccaata aactcactat
     9421 tggtcgaaaa atttacgctc ttggtggtaa tccggatgca acctcccgta ttgggtttaa
     9481 tctcttattt ctccatctat ttgcatacgg ttatatgggc tttatggcag gcttggcagg
     9541 catagtgcaa tcttataccg tacaatctgt cgcaccggat tcactactgg gttatgaatt
     9601 aaccgtccta gctgccgttg tattaggtgg tgctagttta tcaggtggta gaggcaccct
     9661 gcttggtaca gtaatgggcg taatgttact ggcgattatt caaaacgggc taaatttaat
     9721 gaatgtatcg tcttactggc aaaccattat taccggtatc atcattatct taagtattag
     9781 tgcaacagcc ttaagtcagc ggaaaaaaca aggagcataa cgatgaaaag taaactggat
     9841 ccaacgattt tctatctctt agcactcttc gcagttgcag ttatcgcctt tagtattttc
     9901 attccggata ttttttggtc tgtcagtaac tttcaatcta tggcatcaca aattccagta
     9961 ttaggtattt taaccctagc aatggcatta ccgatgttaa caggtggaat aaatctttcc
    10021 attattgccg gaatgaatgc ttgctcatta attattgcct actttattac gcaatatacc
    10081 ggtatttctt ggttatttat tgctatttta ttaagccttt gctgtgcctt agccattgga
    10141 ttactcaacg gcacattaat tgccattatt cgtgtttcac caattttggc aaccttaggc
    10201 acaatgactt taattaacgg cattaatatt ctagtcacta acggttcaac cattgcgaac
    10261 tttccggaaa caatgttgag tattaatgct tcagcctttt tatggattcc gctacccatt
    10321 ctaatctttc tctttcttgc tgggctgatt tggtttttct tagaaaaaac tgcaatgggt
    10381 aaagcggttt atttcatcgg caataatgaa aaagccacct attattcggg tattaatacc
    10441 aaaaaagtgc tgatttgggt ttatgttatc tcctcaattc tttgtgtatt tgcagctatt
    10501 ttgatgatgt ctaaactcaa ttctgcaaaa gcctcttatg gtgattccta tttgttgatc
    10561 tccattttag ccgcagtatt gggcggtatt aaccctgatg gaggtaaagg gaaactatta
    10621 gggattatgc tcgccttagt tttattacaa gtgattgaaa gtggcttaaa tatgctcggc
    10681 attagcagtt acattacaat gattttatgg ggaagcttat tgttattctt tatcttcctc
    10741 caaaaaaatc gccttttccg actaacacag taatggtggt agattatgca aaatgaaaaa
    10801 caactcattg aacaaggtaa tactgttatt ggtattgaat tgggctcaac ccgaattaaa
    10861 gcggtgttaa ttagctcaga tgggacgatt ttagcaaccg gtggggcgga ttgggaaaat
    10921 cgactgattg acggtatttg gacctatcat caaaatgaaa tatgggaaaa actacaagct
    10981 gcttatatgg atttaagcaa gacagtcaaa gaaaaatatc acacaaccat tcgcacagcc
    11041 aaagcattgg gaattagtgc gatgatgcac ggctacttac ctttcgataa acacggaaat
    11101 ccgctggttg cctttagaac ttggcgaaac aacattaccc taacctcatc ggaaaaattg
    11161 accgcactct ttcaataccc aattccgcaa cgttggagta ttgctcattt acaccaagca
    11221 ttgcttaacc aagagcctca cttgccggaa attgattacc tgacaaccct ctcaggttat
    11281 gtgcattggc aactgaccga tcacaaagtg ttagggattg gcgatgcttc cggcatgttc
    11341 ccgatagata ctgagttgaa atcctacaat ccaacaatgc tggctcaatt tgatcgagcc
    11401 attgccgact accgtatgcc ttggaaattg gaagcgattt tgccacaagt tcgtgttgct
    11461 ggtgaacaag cgggcaaatt aaccgaaaaa ggtgcaaaat tgttagatcc aaccgataat
    11521 ttgcaagctg gcattccact ttgtccacct gagggtgatg ccggaacagg tatgattgca
    11581 accaactgta tcaaagaaaa aaccggtaat atttctgccg gcacctccgc atttgcaatg
    11641 attgtgttag aaaaagccct atccaaagcc tattcagagc tggatattgt gacgaccccg
    11701 gcaggtaaat tagtggcaat ggctcacgct aataattgca cctctgatat taatgcgtgg
    11761 gtgaatttat tcggcgaatg cttatcggtt tttggcgtaa atatctgcac cgaagaactt
    11821 tatgaaatgt tatttctcca tgccttgcaa ggcgatgcag attgtggagg gctattatcc
    11881 tacggcttcc actccggtga acataatgtc ggtttatccg aaggctgtcc ggttttcttg
    11941 catccaacga atgcccattt taacttagca aactttatgc gaacccatct ctataccgct
    12001 tttggtgcaa tgaaattagg aatggacatt ctcattaacc aagaaaaagt ggaaatttcc
    12061 cgtattctcg gtcacggtgg tatatttaaa acagaaggtg ttgcacaaaa aattctgtct
    12121 tccgcactga atattccatt ggctaccgca agtactgctt caaatggcgg agcttgggga
    12181 attgcattac tggcagactt tctcagcgta tcagaccgtt acagtttaga agaatattta
    12241 gataattgca ttttcaacac caccaaactc aatctggttc aaccggataa aacaatgagt
    12301 gaaggttatg aacgctttat gcaacgttat aagcaaggca ttcctgtggt agaaaaagcg
    12361 ttgtttttaa atacagatta aataattata atttaagtat cacaagcggt tatttttgca
    12421 aaaaacgacc gcttgttatt tacatttgta caaaaaatac ctactataaa gacgcatttg
    12481 taaaatgtgc attgatatat cgcaagtttt gtgtatagga aatgaactaa ttactgtgtc
    12541 aatcagtccc tttcttcatc gttataccct cttgcaaatc cgattaatct atccttagtt
    12601 aaggtgtttt atgttagcga tttggcaaag ttttaaacaa aattatctaa tcaaatattg
    12661 gaatcctgtt gcagcagtga tcgcagctgg tattatttca gcttattatt tcggcattac
    12721 tggcacttat tgggcggtta caggggaatt tacccgttgg ggcggtcatc tgttaaatgc
    12781 tttgggtgtt gatgtttcga actggagcta ttttcaactc atcggcttag acggcaccat
    12841 ctttacccgc attgacggtg tgatgattct cggtatgttt gccggctgtc tttctgccgc
    12901 attatgggcg aacaacgtca aatggcataa tcagccacat aaaaaacgga ttatccaagc
    12961 cttgattggc ggtgcgttag ccggtttcgg agcccgttta gcaatgggct gtaacctcgc
    13021 ctctcttttt accggtattc cgcaattctc cgtccacgct tggtttttca cctttgcgac
    13081 cgccatcggc acttactttg gggtaaaagt gactttatta ccgatgttta gagtgaaatt
    13141 agagctgaaa aaaggggcag caaaaatcaa agaaaacgat ccgaaacaag ctaatcgccg
    13201 tttttgggtt ggtatgctca tttttatcac ttatctttct gcttcccttt atgtaatgca
    13261 atcctcaatc aaacttggtt ttgcaatgct ttgcggctta gcctttggct tattgattga
    13321 acgagcccaa atttgcttta cctccgcttt ccgtgactta tggattaccg gacgggctta
    13381 tatggcaaaa gcaattattt tcggcattat tgccggcaca atcggcgtgt tctcttatat
    13441 tcaattaggt gtttcaccta aaattttgtg ggctggccca aatgccatta tcggcggatt
    13501 attattcgga tttggcattg tgcttgccgg cggctgtgaa accggctgga tgtaccgttc
    13561 aatggaagga caggttcatt ttatgtgggt cggcgtaggc aatgtagtcg gttcaaccct
    13621 gctggcttac tactgaggcg acttagctcc ggtgcttacc cttgattatg aaaaaatcaa
    13681 cctactcaaa gaatttgggc cggttggcgg attattcatc aattatggct tattaattct
    13741 ttgcttaatc gctgtagttt ggtgggaaaa acgcttcctt gccaaagcga aagcaaaatt
    13801 agcaactgcg taatcttttt aacttcaacc aacaagagga aaatgactat ggcattcacc
    13861 gttgtacaaa aactagataa agatccaaaa gacatcacgc ctgattacac cttagatacc
    13921 ttaggcgaac cttgccctta ccctgcgatt acgatgttag aaacaatgcc acagctgcaa
    13981 aaaggagaga tattggaact gttaagcgat tgttcccaat cgattaataa tattccgatt
    14041 gatactaaaa atcacggcta taccctatta agcgtggaac aaaacggcac taaattacgc
    14101 tatttaattc agcgttaatt gcaaaaaatt cgcacaaaac gaccgcttgt cgtaaaatag
    14161 caactattta agagccttgt aacgcaaggc tcttttattc taagaggtaa aagaaaaatg
    14221 aataaatacg ctttacttgc aatattttgg tttattattg gcatttatgc tttacttttt
    14281 cgtgaaagca ctaatgatat accgcctttt ccacattttg ataaagtaag ccattttgcc
    14341 ttattcttta tccagatctg gttatctgca aacgccttta tccgttcaaa gaaagggata
    14401 ccttattttg ggctaataat ttttgcctta ctctatgctt tcaccagcga atgggctcag
    14461 gcaaccttca cctcaacccg agaaggttct tggcttgatg ggctagcaga tatgctagga
    14521 gcctcacttt cattattgct ggtaaaatat tgcaaaaaat aaagcataaa agaccgcttg
    14581 ttatatggtt tagccatact acaagcggtc attttttacc aaaattatgc catcaactca
    14641 tcaatcaatt ctttcacact tttgattttg ttgatttgtg aaatgccatt gcccaccgac
    14701 acatagccat tgtcaaaatc cccttctaac atacccaaac gcattcctgt accagcgttc
    14761 attaccttag caatctcact acgagttgcc ccattttcgc tcatttcaaa gcattttttc
    14821 gctaatccag tcggcagtga acggtagtaa tgtggttcgg tacggaataa cagcagatca
    14881 ttggcatttg cctccacaat ccagtttttc actttttcgc ttgcacggca ttcttcggtg
    14941 gcgataaatg ccgtgccaat ccacacgcct tcagccccaa gcgttaaggc agcctgcacc
    15001 gcttttttat cggcaatacc accagccagc attatcggca aatccacctt gcccacaaat
    15061 tccgccagca cattaaaact gcctacctct ttcatcggca aagtaccgcc ctcatcaaaa
    15121 cccgtcagca ccaaaatatc tgctcccaat gcttgggctt ttaaagcgtc cgccaaattg
    15181 ggattaggca aacggcagat gattttaatg ccagcagctt tgaatttggc gtaaatctct
    15241 gggtcaagcc agccgttatc cagcaccaca ggcacacgct cttcaatgaa taaatccacc
    15301 attaacggaa tcagcgaaaa atcataagac aaaataaatg gtacgccaaa aggcttatcg
    15361 gtcaatgcct taaccttacg aatctcgtta cgcatacggt caagtacttc ttcgttggaa
    15421 gttggattgg tggtttgccc tgcgtgtggg gctaaaaaac caagcccacc agcctcggca
    15481 acgcttgcta ccagatgagc gtccgtcagc caagacattg gcgcttggac gatagggtgc
    15541 ttgatattta agatttgggt aatgcggttc attggatttt cctgtttgaa taaaaagaat
    15601 tggacgtagt ttaatggttt tggatttgag agggaatgcg tgaaaatggg aaggtggttt
    15661 tcggatttgg aaaattatca atgctaaaat acttgaaata cacacgccaa tcctcatatc
    15721 aacaaaattg aatatttaat tatggtaaaa gaaaatgtct attagatggg aaaacatcaa
    15781 gcccttacaa ggttctcaaa atagtgcttt tgaagagcta gtatgccaat tggcacgcca
    15841 agaatttaaa tctcaaggaa aatttactcg aatttctgca cctgatggtg ggattgaagc
    15901 aatgtgtgaa ttgccagatg gttccgtatg tggttggcaa gccaaatatt ttttaagctc
    15961 attttcttca tcacaatggc aacaaattga agattccttt aaagaatctt taaaaaacta
    16021 tcctaaacta accaaatact atgtttgtgt ggcaaccgat agagcaaatg ccaatgtatc
    16081 tggaaagaaa tcatttctta ccaaatggga ggagtatacc caaagatgga aagactttgc
    16141 tcaatcacaa ggtcgtgaaa tagaatttga attttgggga agttttgaat tatctgactt
    16201 attatcaaaa cctgaaaatg caggcaaaaa attcttttgg tttaacgaaa gcgaattatc
    16261 tgacaaatgg tttgaacaat acaatcaaca agccattaat aatcttggtg tgcgttatac
    16321 acctgaaatt aatgttgatt tacctattgc aatgcaatta gaaagcttgg caagaaccca
    16381 aattttcaaa aaacatctga g
//
LOCUS       DANLRK010000043        16225 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.043, whole genome shotgun sequence.
ACCESSION   DANLRK010000043 DANLRK010000000
VERSION     DANLRK010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 16225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16225
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.043"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            49..2271
                     /gene="glgB"
                     /locus_tag="PXA33_000987"
     CDS             49..2271
                     /gene="glgB"
                     /locus_tag="PXA33_000987"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HDL5120331.1"
                     /translation="MQDYTYSEKDAFFVERLSSGSCNDPFSYLGIHPSNNGVIIRVFL
                     PSAIACQVIDLNGKPVSSMLKIDDRGFFIAELIGKKTDFSYRLLVEYPDSTVDIEDPY
                     RFKTHIQELDNWLFAEGKQLRPYEKLGAHLVTQNEVGGVHFSVWAPNAQRVSVVGDFN
                     HWNGLVHSMRFHPDSGIWDIFLPNVKKGECYKFEVLDRNGVLRLKSDPYAFASQFRPE
                     TASIVSGLPSIVPAEKTCRNANAPDKPISIYEVHLGSWRRNIENNYWLSYEELADELI
                     PYVKDMGFTHIELLPISEFPFDGSWGYQTTGIYSPTSRFGTPEGLSYFIQKAHDKGIG
                     VILDWVVGHFPVDEHGLVYFDGTHLYEHSDPKEGYHKDWNTLIFSYGRNEVSNYLSGN
                     ALYWIERFGIDALRVDAVASMIYRDYSRSDGEWIPNKYGGRENLEAIDFLRHTNNILR
                     DESQGGASIAEESTSFAGVSHSTERGGLGFNYKWNMGWMNDTLRYMQKDPIYRRYHHN
                     LMTFGMTYQYSEQFVLPLSHDEVVHGKGSLLNKMPGDCWQKFANLRAYYGYMWGYPGK
                     KLLFMGNEFAQGKEWNFAESLDWYLLGEEGGEWHKGVLGWVKALNHLYTQTPALFEQD
                     YNPEGFEWLVVDDHDNSVFAFERKAKNGDSVIVVSNFTPIVRENYRFGVNELAEYQEI
                     LNSDLACYCGSNVSNANRFASELIASHGKAYSLSITLPPLSTIFLKRAADKKTKQNKT
                     "
     gene            2295..4307
                     /gene="glgX"
                     /locus_tag="PXA33_000988"
     CDS             2295..4307
                     /gene="glgX"
                     /locus_tag="PXA33_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HDL5120332.1"
                     /translation="MHYFRGKPFPLGNKLDRQAGEFGTNFAIVSQNATAIELCLFDEN
                     HHETRIPMFSSGDTWHIFVAGVQAGTKYGYRVKGIQDEQAGLLFNPQKLLTDPYAKAI
                     EGKPDLSSAEAAAWYKWNDERDNASLAPKSIVIDEHFDWENDSQLFTPWAETIIYEMQ
                     VKGFSQRNFQIPSDIRGTFAGLAHPVSIDYLKTLGITAVELLPVTYHIDETHLQKQGL
                     VNYWGYNVLGNFAIDPNLAADKQNPLKEFKQLVKTLHQAHIEVIMDIVFNHTAESDKN
                     GPMLSFRGIDNTAYWLDKQGEYENWTGCGNTLNLSNPYILCWAIDCLIYWVEECHIDG
                     FRFDLASILGRTPSFSEKADFFTALKSHSKLTHIKLIAEPWDIGVEGYQLGQFPEPFY
                     QWNDCYRDDIRRFWLQNQGDWGLLARRLAGSDDVFTKYSVSKSINFITAHDGFTLQDL
                     TSYQQKHNWANGEQNRDGHNSNYSNNHGVEGQTADELVKKQREITACSLLATLFLSAG
                     VPMLLAGDEIGHSQQGNNNSYCQDNEITWLDWENMNHTRLDYVRSLIRLRKKIALLSK
                     QNNWWQEDSVQWLNPSAQLMNIDDWHNQHNGLVVLLQEEWLLLFNANRQPQPFLLPQG
                     NWKVVLEQAQGILMTDTQAVTLNNMGVCVLQKSDVMFANKLETQYA"
     gene            4300..5613
                     /gene="glgC"
                     /locus_tag="PXA33_000989"
     CDS             4300..5613
                     /gene="glgC"
                     /locus_tag="PXA33_000989"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HDL5120333.1"
                     /translation="MLNQESTALVARQDLTNDTLVLILAGGRGSRLYELTDKRAKPAV
                     YFGGSRRIIDFALSNCINSNLLKIGVITQYAAHSLLRHLQRGWSFLPYERNQYIDMLP
                     ARQQLDENTWYRGTADAVYQNMAMMKSHYRPKYVVILAGDHIYKMDYTQMLKDHVDQG
                     AKCTVGCIEVPRSQATEFGVMAVNEKLKVKEFVEKPADPPAMPGKPDSSLASMGIYVF
                     NADYLYEMLEKEASTPGTSHDFGKDIIPKAVEQGVLYAHPFERSCKGRNASGAIYWRD
                     VGTIDSYWAAHMDLVSEDPELDLYDESWPIHGRPQQTAPARFFYNRARARTLDNSLIA
                     GGCVITDAEISNSVLFNRVHVEAESVIEHTVILPQVTIGKNCVIKRAVIDRHCVIPDG
                     LEIGVNAEEDAKRFRVSKNGIVLVTQSMLDKLAGKEANSDIKYTI"
     gene            5736..7172
                     /gene="glgA"
                     /locus_tag="PXA33_000990"
     CDS             5736..7172
                     /gene="glgA"
                     /locus_tag="PXA33_000990"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017357702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HDL5120334.1"
                     /translation="MKVLHICSEMYPLIKTGGLADVMGALPFAQQQAGNDVRVLIPYY
                     PKVAQTLGETVEVATIGTFAGTVSVRFAYFNGLGVYVIDAPHLFMRDGNPYHDSNYQD
                     YPDNYKRFALLGYIGAQLAQGLDHWWGTAQVLHAHDWQAGLACAYLKSWGSNVKTLFT
                     IHNIAYIGRFNAFHLAELGLPWEFFHAEGLEFYGEISYLKAGLYFADRITTVSPTYAR
                     EITEEIAGAGLHGLLATRQYQGRLRGILNGVDAEVWNPETDTHIVVNYRANYMHGKAK
                     NKAELQRIFGLPEDKEALLMVMVTRLTEQKGADFLLANIDTLMQHKVQIIVLGSGSPD
                     LENALRYFQHKYPNQIGVKIGYDEALSHQIIAGGDLILVPSRFEPCGLTQLYGLKYGT
                     LPLVRRTGGLADTVVDSHKESIENRSATGFVFDYPDAPAFLEAAMRAISLWQKQKLWS
                     SVRQNALAQDFGWAKVAEQYQNLYHEII"
     gene            7267..9767
                     /locus_tag="PXA33_000991"
                     /pseudo
     CDS             7267..9767
                     /locus_tag="PXA33_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651737.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
     gene            complement(9854..10570)
                     /gene="trmJ"
                     /locus_tag="PXA33_000992"
     CDS             complement(9854..10570)
                     /gene="trmJ"
                     /locus_tag="PXA33_000992"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HDL5120335.1"
                     /translation="MQILNQIQIILVETSLPANIGSAARAMKTMGLTHLRLVAPKQPL
                     DEQAEALAAGAKDVLDNAQCYATFDEAIADCQLVIGTSARLRHLQNTLIEPRDCGELV
                     VNRTNLGKVAIVFGRERVGLTNEELLKCQYHLNFPTNPDYGSLNLAMAVQLASYEIRM
                     AYLQNSGEKQPLAADKDYPTAQALEHFFAHTERLYTELGFIRNDSVMLKLRRLYQRAE
                     LETNELNLLRGMLTAVEKAN"
     gene            10750..12288
                     /gene="pntA"
                     /locus_tag="PXA33_000993"
     CDS             10750..12288
                     /gene="pntA"
                     /locus_tag="PXA33_000993"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007525314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HDL5120336.1"
                     /translation="MLIGVPRELLDGETRVAATPKTVEQIKKLGFDVLVEQDAGFKAS
                     FEDNTFLNAGANIGTQQEVWNADIIFKVNAPTEAEIELIKEGVTLVSFIWPAQNPQLM
                     EKLQSKKINVLAMDAVPRISRAQALDALSSMANISGYRAVIEAANAFGSFFTGQITAA
                     GKVPPAKVLVIGAGVAGLAAIGAANSLGAIVRAFDSRPEVKEQVKSMGADFLEIDFEE
                     EGGSGDGYAKVMSEEFNRRAMALYAEQAKEVDIIITTAAIPGKPAPRLITKEMVDSMK
                     PGSVIVDLAAATGGNCEYTKAGEIVVTDNQVKVIGYTDFPARLPTQSSQLYGTNLVNL
                     LKLLAPNKDGQIEINFDDVVLRGVTVVRDGELTWPAPPIQVSAQLQKQAAAPQAVKKE
                     EKPADPRVKYGVMAAAGVLFLWLASVAPAAFLSHFTVFVLACVVGYYVVWNVSHALHT
                     PLMAVTNAISGIIIVGAVLQIAQPTGNFFIDILAFIAILVASINIFGGFKVTQRMLAM
                     FRKG"
     gene            12303..13748
                     /gene="pntB"
                     /locus_tag="PXA33_000994"
     CDS             12303..13748
                     /gene="pntB"
                     /locus_tag="PXA33_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HDL5120337.1"
                     /translation="MSFGFVTAAYIIAAILFIMSLAGLSKHETAKAGCWYGIVGMAIA
                     LVATIFGPQSHGTIWILIAMAIGGFIGVKKALKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYGLHTNALMPANLDETAQAAFLAEQATLANIHNVEVFLGIFIGAVTFSGSLVAFG
                     KLSGKLFGRKVSSAALNIPHKHKWNLAAIIVSVFLMIVFLNNPHNIFPVLIMTIIALV
                     FGWHLVSSIGGADMPVVVSMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYI
                     MCKAMNRSFISVIAGGFGNEVQSGKGDEEYGEHRETKAEEVAEMLKNASSVIITPGYG
                     MAVAQAQYPVAEITAKLREKGVNVRFGIHPVAGRLPGHMNVLLAEAKVPYDVVLEMDE
                     INDDFEETDVVLVIGANDTVNPAALDDPSSPIAGMPVLEVWKAQNVIVFKRSMAVGYA
                     GVQNPLFFKENTQMLFGDAKERVDDILRALN"
     gene            complement(13815..14741)
                     /locus_tag="PXA33_000995"
     CDS             complement(13815..14741)
                     /locus_tag="PXA33_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL5120338.1"
                     /translation="MKPIFLEIRHLRTLIALKESGSVSLAAKRVHLTQSALSHQIKLL
                     EEQYDLTLFERKSSPIRFTPAGERLIKLAYDILPKVIEAELDLARVKQGEVGEVRVAV
                     ECHTCFDWLMPAMDTFRQHWPLVELDIVSGFHTDTVGLLLSHRADLAIVAEEEDSAGI
                     IYKPLFSYEMVAICSKDHPLATKDVWEAEDFKDETLITYPVPDDMLDLLRKILGPAGI
                     KVTRRNSELTIAIIQLVASRRGIAALPYWAVKPYLDRGYVVARKITQQGLYSTLYGAI
                     READATLAYLDDFHDTVKSQSFATLPGLMVLE"
     gene            complement(14851..15966)
                     /gene="asd"
                     /locus_tag="PXA33_000996"
     CDS             complement(14851..15966)
                     /gene="asd"
                     /locus_tag="PXA33_000996"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017357680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HDL5120339.1"
                     /translation="MQNVGFIGWRGMVGSVLMDRMVEENNFANINPVFFTTSQAGQKA
                     PVFGGKDAGELKNAFDIEELKKLDIIVTCQGGDYTNEVYPKLKETGWNGYWIDAASAL
                     RMKDDAIIVLDPVNQHVISEGLKNGIKTFVGGNCTVSLMLMAIGGLFEKDLVEWVSVA
                     TYQAASGAGAKNMRELLSQMGELEDSVKAELANPASSILDIERKVTAKMRAEDFPTEN
                     FGAALGGSLIPWIDKLLPETGQTKEEWKGYAETNKILGLSDNPIPVDGLCVRIGALRC
                     HSQAFTIKLKKDIPLAEIEQIIAAHNEWVKVIPNDKETTLRELTPAKVTGTLSVPVGR
                     LRKLAMGGEYLAAFTVGDQLLWGAAEPVRRILVQLTQ"
BASE COUNT         4668 a         3125 c         3700 g         4732 t
ORIGIN      
        1 gagaactatt gaaatttaag tattaaacgc tttaagcaag gagtgccgat gcaagactat
       61 acttattctg aaaaagatgc tttttttgtt gagcgacttt cttcgggttc ttgtaatgat
      121 cctttctcct acctaggtat tcaccctagc aataacggcg ttattattag ggtatttctt
      181 ccttctgcca tagcttgcca agtgatcgat ttaaacggaa agcctgtgtc atcaatgcta
      241 aaaattgatg atagaggctt ttttattgca gagctgattg gtaaaaaaac agatttttct
      301 taccgcttgt tggtggaata ccctgattca acggtggata tagaagaccc ttaccgtttt
      361 aaaacccata ttcaagagct tgataattgg ttgtttgcgg aaggaaaaca acttcgcccg
      421 tacgaaaaat taggcgcaca tttagtcaca caaaatgaag ttgggggtgt gcattttagc
      481 gtctgggcgc cgaacgctca gcgggtttcc gtggttgggg atttcaacca ctggaacggc
      541 ttggtacatt caatgcgatt ccaccccgat tccggtattt gggatatttt cctgccgaat
      601 gtgaaaaaag gggagtgcta taaatttgaa gtgttggatc gaaacggcgt gctacggttg
      661 aaatccgacc cttacgcctt tgcctcccaa ttccgcccag aaaccgcatc tattgtttcc
      721 ggcttgccaa gcattgtgcc agccgagaaa acctgccgta atgcgaatgc tcccgataaa
      781 ccgatttcca tttatgaagt gcatttaggc tcttggcgac gcaacattga aaacaattat
      841 tggcttagct atgaagagct tgccgatgaa ctcattcctt atgtgaaaga tatgggcttt
      901 acccatattg aattgctgcc tatttccgaa tttccgtttg atggctcttg gggttatcaa
      961 accaccggta tttattcgcc aaccagccgt tttggtacgc ctgagggctt gagttacttt
     1021 atccaaaaag ctcacgataa aggcattggt gtgattcttg actgggtagt ggggcatttt
     1081 ccggtagatg aacacggctt ggtttatttt gatggaacgc atttatacga acattccgat
     1141 ccgaaagagg gctaccacaa agattggaat accttaattt ttagctacgg cagaaatgag
     1201 gtgagcaatt atttgtctgg taatgcactt tattggattg agcgttttgg gattgatgcg
     1261 ttgcgggtgg atgcggtggc gtcaatgatt tatcgtgatt actcacgttc agacggcgag
     1321 tggattccaa ataaatatgg cggacgtgag aacttagagg caatcgactt cttacgccac
     1381 accaataata ttttgagaga cgaaagccaa ggcggtgcga gcattgcaga ggagtccact
     1441 tcttttgccg gagtgtctca ctcaactgaa agaggcggtt tgggctttaa ttataaatgg
     1501 aatatgggct ggatgaacga cactttacgc tatatgcaaa aagatccaat ttaccgccgt
     1561 tatcaccaca atttaatgac ttttggtatg acctaccaat acagcgaaca gtttgtgtta
     1621 ccgctttcac acgatgaagt tgttcacggc aaaggttcat tgctgaataa aatgccgggg
     1681 gattgctggc aaaaatttgc gaacttacga gcctattatg gctatatgtg gggctatccg
     1741 ggcaaaaaat tgctctttat gggcaacgag tttgctcaag gtaaagagtg gaattttgcc
     1801 gagagcttag attggtattt actcggggaa gaagggggcg aatggcataa gggcgtattg
     1861 ggttgggtaa aagcccttaa ccatttatac acccaaaccc ctgcactgtt tgagcaagat
     1921 tacaatcctg aaggttttga gtggttagtg gtggacgatc acgataattc ggtgtttgct
     1981 tttgagcgta aagccaaaaa tggcgattcg gtgattgtgg tgagtaattt caccccgatt
     2041 gtgcgtgaaa attaccgttt tggggtgaat gaattggcag aatatcagga aattctcaat
     2101 tctgatttag cttgttattg cggtagtaat gtcagcaatg caaaccgatt tgccagcgag
     2161 ttaattgcat cgcacggtaa agcctactcc ttgagcatta ctttgccacc gctatcgact
     2221 atcttcctca aaagagccgc agacaaaaaa acaaaacaaa acaaaacata acttaaagaa
     2281 acataaaaaa taatatgcac tattttagag gtaagccttt tcctttaggc aataaacttg
     2341 atagacaagc gggtgaattt ggcacaaatt ttgccattgt ttcgcaaaat gcaaccgcta
     2401 ttgagttatg tctttttgat gaaaatcacc acgaaacccg tattccgatg ttttcttccg
     2461 gagatacttg gcacattttt gtagcaggtg tacaagcagg gacaaaatat ggctacaggg
     2521 tgaaaggcat acaagatgaa caagccggtt tgttatttaa tccgcaaaaa ttattgactg
     2581 atccttacgc taaagcgatt gaagggaagc cggatttaag tagtgcagag gcagccgctt
     2641 ggtataaatg gaacgatgaa cgggataatg cctctcttgc acctaagtct atcgtgattg
     2701 atgagcattt tgattgggaa aatgatagcc agttattcac tccttgggca gagactatta
     2761 tttatgaaat gcaggtgaaa ggttttagtc agcggaactt tcagattcca agcgacattc
     2821 gaggcacttt tgccggtttg gctcaccctg tttcgataga ctatttaaaa accttaggaa
     2881 tcaccgcagt agaacttcta cccgttactt accatattga tgaaacccat ttgcagaaac
     2941 aagggcttgt gaattattgg ggctataatg tattaggtaa ttttgctatt gatccgaatt
     3001 tagcagcaga taagcaaaat cctcttaaag agtttaaaca gctcgtaaaa acattacatc
     3061 aagcacatat cgaagtgatt atggatattg tgtttaacca cacagctgag agtgataaaa
     3121 acgggccgat gctaagtttt agaggcattg acaatacagc ttattggcta gataaacagg
     3181 gcgaatacga aaattggact ggctgtggta ataccttaaa tttgagcaat ccttatatac
     3241 tctgctgggc aatcgattgc ttaatctatt gggtggagga gtgccatatt gatggattcc
     3301 gctttgatct tgcctcaatt ctaggtagaa caccgagttt ttctgaaaaa gcagattttt
     3361 tcacagcact taaaagccat tctaaattaa cacatattaa gttgattgct gagccttggg
     3421 atattggtgt agaaggttat caattagggc aattccctga gcctttttat cagtggaatg
     3481 attgttatcg tgatgatata cgccgttttt ggttacaaaa ccaaggtgat tgggggcttt
     3541 tggcacggcg attggcagga agtgatgatg tgtttactaa atattccgtt tcaaaaagta
     3601 tcaattttat tacggcacac gatgggttta cgttacaaga tctcaccagc tatcagcaaa
     3661 aacataattg ggcaaatggt gaacaaaacc gagacgggca taatagtaac tacagcaata
     3721 accacggtgt tgaagggcag acagcagatg aattggtaaa aaaacagaga gaaatcaccg
     3781 cttgtagttt gcttgctacg ctctttttat cggctggtgt tccaatgctt ttagccggtg
     3841 atgaaatcgg acatagtcaa caaggtaaca ataatagtta ttgccaagat aatgagatca
     3901 cttggctaga ttgggaaaat atgaatcaca cacggctgga ttatgttcgt agtttaatcc
     3961 ggttgcgtaa aaaaatcgct ttgctttcca agcagaataa ttggtggcaa gaggattcgg
     4021 tacaatggtt gaatccttct gctcagctaa tgaatattga cgattggcat aatcaacata
     4081 atgggcttgt cgttttatta caggaagaat ggctgctttt attcaatgct aatcgacaac
     4141 ctcaaccgtt tttactgcca caaggcaact ggaaagttgt gttggagcag gctcaaggta
     4201 ttttgatgac agacacgcaa gcggttacgc tcaataatat gggtgtatgc gtacttcaaa
     4261 aaagtgatgt aatgttcgct aataaattgg agacacaata tgcttaacca agaatcaacg
     4321 gctcttgttg caagacaaga tttaaccaac gataccttag tattaattct tgcaggagga
     4381 cgaggttctc gcctatacga attaaccgat aaacgtgcaa aaccggcagt ctatttcggt
     4441 ggtagccgcc gtattattga ttttgcgtta tcaaattgta ttaactcgaa tttattaaaa
     4501 atcggggtga ttacccaata tgcagcacac tctttgttgc gccatttaca acgtggttgg
     4561 tcatttttac cttatgaacg taaccaatat attgatatgt taccggcacg ccaacagcta
     4621 gatgaaaaca cttggtatcg tggcacagcg gatgcggtgt atcaaaatat ggcgatgatg
     4681 aaatcgcact atagaccaaa atatgtggtg attttagcag gggatcatat ctataaaatg
     4741 gactacactc aaatgctgaa agatcacgtc gatcaaggtg caaaatgtac tgtaggttgt
     4801 atcgaagtgc ctcgctcgca agcaacagag tttggggtaa tggcggtgaa tgaaaagctg
     4861 aaagtgaaag agttcgtcga aaaaccagct gatccacctg caatgccggg aaaaccggat
     4921 tcctcgctag catcaatggg gatttatgta tttaatgcag attatctata tgaaatgctg
     4981 gaaaaagaag cctctacacc gggaacctca catgatttcg gtaaagatat tattccaaaa
     5041 gcagtagagc aaggtgtgct atatgcccac ccatttgaac gttcttgtaa agggcgtaat
     5101 gccagtgggg caatttactg gcgagatgtc ggtacgattg acagctactg ggctgcccat
     5161 atggatttag tttctgaaga tccggaatta gatttatatg atgaatcttg gccgattcac
     5221 ggtcgtccgc aacaaactgc accggcacgt tttttctata atcgtgcgcg tgccagaacc
     5281 ctagataact cactgattgc gggtggttgt gtgattaccg atgcggaaat cagtaattcg
     5341 gtcttattta accgtgttca cgttgaagcg gagtcggtga ttgaacacac agtgatttta
     5401 ccgcaggtga ctatcggtaa aaattgcgta attaagcgcg ctgtgattga cagacattgt
     5461 gtaattcctg atggcttaga aattggtgta aatgccgaag aagatgcgaa acgttttcgt
     5521 gtcagcaaaa atggcattgt attagtgaca cagtcaatgt tagataaatt agcgggaaaa
     5581 gaagctaatt cggatattaa atatacaatt tagtaagaga tgatataatg cttcgagttt
     5641 gtcatttgag gtcgcaagct cgaagtattg ttatgattaa agcgcttcaa gcggtcttat
     5701 ttttcaacta ttttacacat agaaaacggt taaaaatgaa agttttacat atttgctctg
     5761 aaatgtaccc tttaattaaa acaggcggtt tagctgatgt aatgggggca ttgccatttg
     5821 cgcaacagca agctggtaat gatgtcagag tgttaattcc ttattacccg aaagtcgcgc
     5881 aaactttagg ggaaacggta gaagttgcta ctatcggcac ttttgccgga acagtctctg
     5941 ttcgatttgc ttattttaat ggcttaggcg tttacgttat cgatgcgcct catttattta
     6001 tgcgtgatgg taacccttac cacgacagca attatcaaga ctatccggat aactataaac
     6061 gctttgcttt attaggttac attggggcgc aattagccca aggtttggat cattggtggg
     6121 gaaccgctca agttttacac gcacacgatt ggcaagcagg gcttgcttgt gcatatctta
     6181 aaagttgggg aagcaatgta aaaacgctct tcactattca taatattgcc tatatcgggc
     6241 gctttaatgc cttccattta gcagagcttg gcttaccttg ggaatttttc cacgccgaag
     6301 gcttagaatt ttacggtgaa atttcttacc tcaaagcagg tttgtatttt gctgatcgca
     6361 ttactaccgt tagcccgacc tatgctcgtg aaattactga ggaaattgcc ggtgcaggct
     6421 tgcatggttt actggcaacc cgtcaatatc aaggtagatt gcgtggcatt ttaaacgggg
     6481 tcgatgctga ggtgtggaat ccggaaacgg atacacatat tgtggtgaat tatcgtgcga
     6541 actatatgca cggtaaagcc aaaaataaag cggaactcca acgaattttt ggtttgccag
     6601 aagacaaaga agcattattg atggtaatgg tgactcgttt aactgagcag aagggggcag
     6661 atttcttact cgccaatata gataccttaa tgcaacataa ggtacagatt attgtattgg
     6721 gaagtggatc gccagattta gaaaatgcgc tacgttattt ccaacacaaa tacccgaacc
     6781 aaattggggt gaaaatcggt tatgacgagg ctttatcgca ccaaattatt gcaggtggtg
     6841 atctgattct agtaccaagc cgttttgagc cgtgcggatt aactcaactt tatgggctga
     6901 aatatggcac tttaccgctg gttcgccgta ccggtggctt agctgatacg gtggtagata
     6961 gccataaaga gagcattgaa aaccgcagtg ccaccggatt tgtatttgat tacccagatg
     7021 cacctgcgtt tttagaggca gctatgcggg caatcagctt atggcagaaa caaaaattgt
     7081 ggtcgagcgt tcgccagaat gcccttgctc aagattttgg ttgggcaaaa gtggcagaac
     7141 agtaccaaaa tttataccac gaaattattt aatttgtatt cacaacatca tttcaagcgg
     7201 tcgaatttgc ggattttttt gtaaaaacga ccgcttgcaa tattaaaaat taggagaatt
     7261 tactcaatga gcaggcagtc attaagccca gaaatgatca aaaatgttaa aaatgcgatt
     7321 ttgcacaaaa tggtgttcgc tttaggggtc gaaccccgtg atgccagtaa gcgtaactgg
     7381 ctcaatgcaa ccttacgtgt agtgcgtgat ttatctaccg aagattggtt acaaacccgc
     7441 cgtagccagg ttgcaaatgg tagccgccgt gtttattacc tttcaatgga atttttaatg
     7501 gggcgtacgt tcagtaacgc aatgatttct gaaggtgtgt atgagcttat tggcgctgcg
     7561 ttaaaagagt tagggcaaga tctggaagag attatcaatg aagagggcgt ccgggcttag
     7621 gtaacggtgg cttagggcgt ttagccgcct gctatatgga cagccttgcc acaatgaaaa
     7681 ttccggcaat cggctacggt attcaatatg aatacggtat gttccgccaa gaaatccgca
     7741 acggggagca aattgagcaa cctgatgaat ggttagagaa tgaatttgtg tggccatatc
     7801 tacgttcaag taagcgtttc ccggtacgct ttggcggtaa aacttggcgt gagggcaaaa
     7861 aagtagtatg gcagccggaa gaagaaatta ccgctcaagc tcacgaccaa ctgatcccgg
     7921 gctttgagac cacctcaact aacagcttac gtttatggtc tgcacacgca tcgggtagag
     7981 ggtttggctt atctgatttt aaccgtggcg attattttgc agcaatggtt aaacaaaaca
     8041 gctcggaaaa cgtctctcgt gtgctttatc cggacgattc tacctataac ggtcgtgaat
     8101 tacgcttacg ccaagagtat ttcctttgct cggcatctgt acaagatatt atccgccgcc
     8161 acgaagcgga gttcggctct tgtgtgaatt tagccgacaa agtggcaact cacttaaatg
     8221 acacacaccc aacccttgcg gtgcctgagt taatgcgtat tttaattgac gaaaaaggct
     8281 atggctggga acaagcgtgg gcgatgactc gtaaaatttt cttctacacc aatcacactt
     8341 tgatgagtga agcgttagaa acttggccgg tagaaatgtt agcccgtgtg ctaccacgcc
     8401 atttagagat tattttcgac attaacgaat atttcttaga ggaagtgcgt agccaattcc
     8461 cggaagatca cgatttgatt tctcgtgttt ccttaattga tgaacaaggc gatcgccgtg
     8521 tgcgtatggc gtggcttgcg gtagtcgcct cagcgaaagt aaatggtgtg gcgaaaattc
     8581 actcggattt aatggttgaa tctatttttg ccgattttgc ccgtatttac ccaaatcgtt
     8641 ttactaatgt gactaatggg gtgacacctc gccgctggat caaaattgcc aacccagggc
     8701 ttgctaatat tttagataaa tgtatcggca ataagtggct gactgattta agtgaattgg
     8761 aaaaattcaa cgtgtttgtt gatgatgctg atgtgcaagc acaggtggca gcggtgaaag
     8821 tagaaaacaa acgtcgctta gccaaatatg tcgaagaaac tcagggaatt aaacttaatc
     8881 ctgaagcgat ttttgatgta caagttaagc gtattcacaa gtataaacgc cagcaaatga
     8941 acgtgttgca tatcatcacg ctttataacc gcatcttgaa aaacccgact gctgattgga
     9001 cacctcgtgt gtttatcttc gcagggaaag cggcaagtgc ttattatgcg gcgaaaaaag
     9061 tgattcgttt gattaatgat gtggcaaatg tggtgaataa tgaccctcgc attaatgatt
     9121 taattaaggt ggtgtttatt ccgaactacg gtgtttcatt agcacaaatg attattccgg
     9181 cagctgatgt ttcagagcaa atttccttag cgggtacaga ggcctccgga acctctaata
     9241 tgaaatttgc cctcaatggt gctttaacca ttggtacatt agacggtgcg aatgtcgaaa
     9301 ttttagatca cgttggtaaa gagaacatct ttattttcgg taatgaagta gagcaggtgg
     9361 aagagcttcg ccacaacggt tactcaccgt atcactacta cgaaaaagac agcgaattga
     9421 atgaggcaat ttcacaaatt ctaaacggta aattctcacc ggaagatccg tatcgctatc
     9481 aagatttgat cttaaattca ggtgactact accaagcctg tgccgattat cgtagctatg
     9541 tggaggctca agaaaaagtg gctcaagcct atcgtaacaa gaaagcttgg acacgttcag
     9601 ccatcatcaa tattgccaat atgggctatt tctcctcaga ccgctcggtg atggattatg
     9661 cgaaaaatat ttggcatatc gaaccgatga gcgaagaaca attaaaaggc gattctacaa
     9721 tgggcagtat tattgaaagt tcaaataaac tgttgaattt aaaataatga tttagagaaa
     9781 acggtaagag taatcttacc gtttttgttt tagtcaggga ttttgcaaat ttctactaaa
     9841 aattaaccgc ttgtcaattt gccttctcca ccgccgtgag cattcccctc agtaaattca
     9901 gctcattagt ttccagttcg gcacgttggt ataaacggcg gagttttagc atcactgaat
     9961 cattgcggat aaagcctaat tcggtatata agcgttcggt gtgagcaaag aaatgctcta
    10021 aggcttgagc ggttgggtaa tccttatctg ctgcaagcgg ttgtttttcg ccagaatttt
    10081 gcaaataagc cattcggatt tcataacttg cgagctgtac tgccattgcc aaattcagag
    10141 aaccataatc aggattggtt gggaaattca aatggtactg gcatttcaat aattcttcgt
    10201 tggttagccc cactcgctca cgtccaaata caatcgccac tttgccaaga ttggttcgat
    10261 ttaccactaa ttcaccacag tctcttggtt caatcagggt attttgcaaa tggcgaagtc
    10321 tggcactggt gccgatcacc agttggcaat cggcaatggc ttcatcgaaa gtggcgtagc
    10381 attgagcgtt atccaacaca tcttttgccc cagcagcaag ggcttcggct tgttcatcaa
    10441 gcggttgttt tggggcgact aatcgcaaat gggttaagcc catcgttttc attgctcggg
    10501 ctgcggaacc gatatttgcc gggagtgaag tttcaacaag aatgatctga atttggttga
    10561 gaatctgcat tggttttgtt tcttttttgt taaatttagc ctgttaatag tgtagcaaaa
    10621 atttagcctg cttaaaattt gaaaaaatgg gaaataatcg gtttaattgg gcataatttt
    10681 ttacctattt gttttattta gtggtacatt tagagcgttt ttaatcacaa caaccataca
    10741 ggagtcttta tgcttattgg tgtaccaaga gagctgttag acggtgaaac tcgagtggcg
    10801 gcaacgccaa aaaccgttga gcagatcaaa aaacttggct ttgacgtact tgtggaacaa
    10861 gacgcaggtt ttaaagccag ttttgaagat aatacatttt taaatgcagg tgcaaatatc
    10921 ggcacacaac aagaagtttg gaatgcagac attattttta aagtgaatgc gccgacagaa
    10981 gctgaaattg agttaatcaa agaaggggta accttagtca gctttatttg gccggcacaa
    11041 aatccacaat tgatggaaaa attgcagtct aagaaaatta atgtgttggc aatggacgct
    11101 gtgcctcgta tttcccgtgc ccaagctctc gatgctttaa gttcgatggc aaatatttcg
    11161 ggttaccgtg cggtgatcga agcggcaaac gcttttggta gcttctttac tgggcaaatt
    11221 accgcagcag gtaaagtacc accggcaaaa gtgttggtaa ttggagccgg tgttgctggc
    11281 ttggctgcaa tcggggcggc aaatagcctg ggggcgattg tgcgtgcctt tgactctcgt
    11341 cctgaagtaa aagagcaagt taaatcaatg ggggctgatt tcctcgaaat tgattttgaa
    11401 gaagaaggtg gctcaggtga cggctatgcg aaagtgatgt cggaagagtt taaccgtcgt
    11461 gcaatggcgt tatatgccga gcaagccaaa gaggtggata ttattattac caccgctgcc
    11521 attccgggca aacctgctcc tcgtttaatc actaaagaga tggttgattc aatgaaaccg
    11581 ggttcagtga ttgttgattt agccgctgca accggtggta actgtgaata caccaaagcc
    11641 ggcgagattg tggtgaccga taatcaagtg aaagtgattg gctataccga ttttcctgca
    11701 cgtttaccaa cgcaatcttc gcaactttat ggtactaact tggtgaattt actcaaactg
    11761 ttagcaccaa ataaagatgg tcaaattgag attaattttg atgatgtagt gttgcgtggc
    11821 gtaacggtgg tgcgtgacgg cgaattaact tggccggctc cgccgattca agtatctgcc
    11881 caactgcaaa aacaagcagc cgcaccacaa gcggtcaaaa aagaggaaaa acctgcagac
    11941 cctcgagtga aatatggcgt gatggcagca gcaggtgtgc tgttcctatg gcttgcatct
    12001 gtggctccgg cagcgttttt atcgcatttt accgtattcg ttctcgcttg tgtggtaggg
    12061 tattatgtgg tttggaacgt cagccacgca ctacataccc cattaatggc ggtaacaaat
    12121 gcgatttcag ggattatcat cgtaggtgcg gtattacaaa ttgcccaacc gaccggtaat
    12181 ttctttattg atattttggc atttattgcg attttggttg ccagcatcaa tatttttggt
    12241 ggctttaaag taacgcaacg tatgcttgca atgtttagaa aaggttaagg agaaacgaaa
    12301 ctatgtcttt tggatttgtg actgctgcgt acattattgc agctattctc tttattatga
    12361 gcttagccgg tctttctaaa cacgaaaccg ccaaagcagg ttgttggtat gggattgtgg
    12421 gtatggcgat tgctttagtg gcaaccattt tcggcccgca atcacatggc acgatttgga
    12481 tcttaatcgc aatggcaatc ggtggattca tcggggtgaa aaaagcccta aaagtagaaa
    12541 tgaccgaaat gcctgaatta gtggcaattc tacacagctt tgtcggttta gcagcggtat
    12601 tggttggttt taacagctac ggacttcata ccaatgcatt aatgcctgca aatttagatg
    12661 aaactgcaca agcggcattt ttagcggaac aagccactct tgccaatatc cacaatgtgg
    12721 aagtgttctt aggtattttc attggtgcgg taaccttctc cggttcattg gttgcctttg
    12781 gtaaactaag cggtaaatta tttggtcgta aagtctcttc tgcagcactg aatattccac
    12841 ataaacacaa atggaatttg gcagccatta tcgtttctgt ttttttgatg atcgtgtttt
    12901 taaataatcc gcacaatatc ttccctgtgc tgattatgac aattattgcg ttagtcttcg
    12961 gctggcactt agtttcttct attggtgggg cggatatgcc ggtggtagtt tcaatgctga
    13021 attcttattc aggttgggca gccgcagcgg caggctttat gctaagcaac gatttattaa
    13081 tcgtaaccgg tgcattagtg ggatcatcag gtgctatcct ttcttatatt atgtgtaaag
    13141 caatgaaccg ctcattcatt agcgtgattg ccggtggttt tggcaatgaa gttcagtctg
    13201 gcaaaggtga tgaagaatac ggtgaacacc gtgaaacgaa agccgaagaa gtggcagaaa
    13261 tgctgaaaaa tgcaagctct gtgatcatca cgccgggtta tggtatggca gtggctcaag
    13321 cacaatatcc ggtagcggaa atcaccgcaa aattgcgtga aaaaggcgtg aatgtgcgtt
    13381 ttggtattca ccctgtggca gggcgtttac cggggcatat gaacgtatta ttggcggaag
    13441 cgaaagtacc ttatgatgta gtgctggaga tggacgaaat caatgacgat tttgaagaaa
    13501 ccgatgtggt attggtaatc ggtgcaaacg ataccgtaaa ccctgctgca ttagacgacc
    13561 catcaagccc tattgctgga atgccggtgc ttgaagtatg gaaagcacaa aatgttatcg
    13621 tgttcaaacg ttcaatggca gtaggctacg ccggtgtgca aaacccactg ttctttaaag
    13681 aaaataccca aatgctattt ggtgatgcaa aagaacgtgt tgatgatatt ttacgggcgt
    13741 taaattaaat ttaaataaaa aagcgaacat aagttcgctt ttttgtgctt aggctaacat
    13801 aaattgttaa ttttttactc taataccatc aatccgggaa gagtcgcaaa actctgcgat
    13861 tttacggtat catggaaatc atctaagtag gctagggtag catcggcttc tcgaatagcg
    13921 ccataaaggg tgctatatag tccttgctga gttatttttc ttgccaccac atacccccta
    13981 tctaaatatg gtttaaccgc ccaataaggt agtgcagcaa tgcctcgccg acttgctact
    14041 agctgaataa tagcaatagt taactcacta ttacgccgag taactttgat gccagcaggg
    14101 cctaatattt ttcgcagtaa atctagcata tcatcaggta ctggataggt aattaatgtc
    14161 tcatctttaa aatcttcagc ttcccataca tcttttgtgg caagagggtg atctttagag
    14221 caaatagcta ccatttcata tgaaaagaga ggtttgtata taataccagc actatcttcc
    14281 tcttcagcga caattgccag atctgcacga tggcttaata gcaaaccaac cgtatcagta
    14341 tggaaacccg atacgatatc taattcaact agaggccaat gttgacggaa agtatccatt
    14401 gccggcatta gccaatcaaa acaggtatgg cattctactg caacacgtac ctctccgact
    14461 tctccttgtt tcactcgggc taaatctaat tcagcctcaa tgactttggg caaaatatcg
    14521 taggccaact taattaaacg ttcgccagct ggtgtaaagc gaatagggga gctttttcgc
    14581 tcaaatagcg taagatcata ctgttcttcc agtaatttta tttggtgtga aagtgcggat
    14641 tgtgttaaat ggacacgctt tgctgcaaga gatacgctac cgctctcttt taatgcgatt
    14701 aatgtccgca aatggcggat ttcaagaaaa atcggtttca taatttattc atctaaaagt
    14761 gaaaaaagat gaaaataatt ataacaaaca agcggtcaaa ttctgcaaaa aatttgcaaa
    14821 atccaaccgc ttgtatagag cagtggcgaa ttattgggtt aattgaacta aaatgcggcg
    14881 aacaggctct gctgcacccc ataataattg gtcacctacg gtaaatgcag ctaagtattc
    14941 tccacccatc gctaatttac gcaagcgacc aaccggcacg ctcaatgtac cggtcacttt
    15001 cgccggggtc aattcacgca atgtggtttc tttgtcgttt ggaattactt tcacccattc
    15061 attgtgggca gcaataattt gctcgatttc cgctaacgga atatcttttt tcagcttaat
    15121 tgtgaatgct tggctgtggc agcgtaatgc tccaatacgc acgcataaac catcaactgg
    15181 aattgggtta tcgcttaaac ctaaaatttt attagtttct gcataacctt tccactcttc
    15241 tttagtttgc ccggtttccg gtaataattt atcaatccaa ggaattaagc taccgcccaa
    15301 tgccgcaccg aagttctcgg ttgggaagtc ttccgcacgc attttagccg taactttacg
    15361 ttcaatatct aaaatagaag aggcaggatt tgctaattca gcttttacgc tatcttctaa
    15421 ttcacccatt tgagaaagca attcacgcat atttttcgca ccggcaccgg aagcggcttg
    15481 gtaagttgct acagaaaccc actcgaccaa atctttctca aataaaccac cgatagccat
    15541 tagcattagg cttacggtac agtttccgcc tacaaatgtt ttaatgccgt tttttaagcc
    15601 ttctgaaatc acatgttggt tcaccgggtc taatacgata atagcatcgt ctttcatacg
    15661 taatgcagaa gccgcgtcaa tccaatagcc gttccaaccg gtttctttta attttggata
    15721 gacttcattt gtataatcac caccttggca agttacgata atgtctaatt tttttaattc
    15781 ttcaatgtca aaggcgtttt ttaattcgcc ggcatctttt ccgccaaaaa caggggcttt
    15841 ttgacccgct tgtgaagtag tgaaaaaaac agggttgata ttggcgaagt tattttcttc
    15901 aaccatacga tccattaaga ccgaaccgac cataccacgc caaccgataa aacctacatt
    15961 ttgcattgtg tttgctccta aaaatgatgt tataaagatt gtgaataaat acaggatttt
    16021 agtgcaaaaa tcaatgtttt ttattgattt gcaaagtttt gacaaaattt aaccgctatt
    16081 gttaaaataa aacatcgatt atggtgtatt tttggaaata aatagaatct tataaatagg
    16141 gaaatgctca caatttatgc attccaattg tggtctaatc aataaatttt gccgatatat
    16201 cgataggaag tagatgtaat tacct
//
LOCUS       DANLRK010000044        16109 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.044, whole genome shotgun sequence.
ACCESSION   DANLRK010000044 DANLRK010000000
VERSION     DANLRK010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 16109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16109
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.044"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            <1..274
                     /locus_tag="PXA33_000997"
     CDS             <1..274
                     /locus_tag="PXA33_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5120340.1"
                     /translation="LDEWYSASDKERREFIAKHFAEETVRSDRNTPYDYAMCTGRMFA
                     KQLTRDEVKDYPLANTKPQYLTRDRRIEIMSNLPREILQNCAKYIQ"
     gene            complement(310..1062)
                     /locus_tag="PXA33_000998"
     CDS             complement(310..1062)
                     /locus_tag="PXA33_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HDL5120341.1"
                     /translation="MAYKPPFTVTTKMLNLVVEISHKLGTLQLDFERNLHLRKNNRLR
                     SIQASLAIEANSLTIEQVTAIIKGKKVLGHPKDIREVKNAYDAYDEMMHYNPYSIKDF
                     LKAHKFLTADLVNDAGAFRSKDVGIYNFSGQLVHMGARPQFVENLINELFSWAKQDDT
                     PALIKSSVMHYEIEMIHPFSDGNGRMGRLWQNVLLAQWNPLFAWLPIETMVYENQQRY
                     YQMLSLADSQNDSTGFIEFMLEMILETLLKLK"
     gene            complement(1101..2348)
                     /gene="clpX"
                     /locus_tag="PXA33_000999"
     CDS             complement(1101..2348)
                     /gene="clpX"
                     /locus_tag="PXA33_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HDL5120342.1"
                     /translation="MAFEKEPHCSFCGKKRTEVDQLVEGTEGYICNECIEESYALLHG
                     EEEPLIDENQDDEGSFFTNVPTPHELHAHLDDYVIGQEHAKKVLSVAVYNHYKRLRSA
                     LSNHQATNGVELGKSNILLIGPTGSGKTLLAETLARRLNVPFAVADATTLTQAGYVGE
                     DVENVIQKLLMKCDFEAEQAERGIVFIDEIDKITRKSESASITRDVSGEGVQQALLKL
                     LEGTVANINPQGGRKHPKGETIPVDTSKILFICGGAFAGLDKIVEARTNTQGGIGFAA
                     ALKKDKDRQDLTELFKQVEPEDLVKFGLIPELIGRLPVVTPLQELDEEALIQILTEPK
                     NAIIKQYQALFEMEGVELHFTKDALISIAQKAISRKTGARGLRSIVENLLLDTMYDLP
                     TLNAKKVTIGKGCVENGDKPKVE"
     gene            complement(2348..2941)
                     /gene="clpP"
                     /locus_tag="PXA33_001000"
     CDS             complement(2348..2941)
                     /gene="clpP"
                     /locus_tag="PXA33_001000"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HDL5120343.1"
                     /translation="MALVPMVVEQTSKGERSYDIYSRLLKERIIFLNGQVEDNMANLI
                     VAQLLFLEAEDPEKDIYLYINSPGGVVTAGLAIYDTMNFIKPDVATLCTGQAASMGAF
                     LLSAGAKGKRFALPHARIMIHQPLGGARGQATDIQIQAEEILRLKATLTRRMAEHSGQ
                     SYEKVLADTERDNFMSAEEAAEYGLIDKVLTSRSEVA"
     gene            3053..3436
                     /locus_tag="PXA33_001001"
     CDS             3053..3436
                     /locus_tag="PXA33_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HDL5120344.1"
                     /translation="MTLTEIYQKFERCKSWEERYRLLIQLSRLLPKPSEQELAQIAEI
                     EGCESRLWFEFQAEPRKVVAYSDARLMQGILFIVSVALTEKTAEELKGFDLKTMFDEL
                     KISQNLTSTRLNGLGQLQKHIMMNL"
     gene            complement(3540..3616)
                     /locus_tag="PXA33_001002"
     tRNA            complement(3540..3616)
                     /locus_tag="PXA33_001002"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3580..3582),aa:Asp,seq:gtc)
     gene            complement(3705..4460)
                     /locus_tag="PXA33_001003"
     CDS             complement(3705..4460)
                     /locus_tag="PXA33_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL5120345.1"
                     /translation="MKKSIYDTPIFFERYQQLRQNPISMNEVVEKPTMFSLLPDLTGK
                     KVLDLGCGTGVHLAHYLTLGAEKVVGLDLSELMLQQAESDLSAHWQKGNDFSLYCLPM
                     ERLNEISEGDFDVVTSSFAFHYIADFSELLAKISQKMTACGTLIFSQEHPIVTCYREG
                     YRWEKNQEKQQVAYRLNYYREEGERDRSWFQQPFKTYHRTMATIVNNLIQGGFEIVQM
                     EEPMLAGQPRWHNEFKDLQHRPPLLFIKAIKQK"
     gene            complement(4520..5515)
                     /gene="trpD"
                     /locus_tag="PXA33_001004"
     CDS             complement(4520..5515)
                     /gene="trpD"
                     /locus_tag="PXA33_001004"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HDL5120346.1"
                     /translation="MQALLDKLFANQALAQAESHRFFSEVIKGNVSNEQLAAALIALK
                     LRGETVDEIAGAAKAALENANPFSTPDYPFADIVGTGGDGHNTINISTTSAIVAATMG
                     YKIAKHGNRSVSSKTGSSDVLSHLGINVDVSPETARLALDETNICFLFAPTYHSGFKY
                     AAPVRQALKTRTIFNILGPLVNPAHAKRQLLGVYSPELVKVYAETVKALGYEHTIVVY
                     GAGLDEVAVHGETQVAEIQNGQIDYYTLTPEDFGIERHSLEDLKGGEPAENAEKVTAI
                     LQGKGKLAHIDAVAANVALLMKTFGEDDLKANVQKVKETLESGKALETLRKLSTY"
     gene            complement(5533..5919)
                     /locus_tag="PXA33_001005"
     CDS             complement(5533..5919)
                     /locus_tag="PXA33_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005543439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HDL5120347.1"
                     /translation="MISVYGLKQTLADRRALIADVIFDCMQMSLGVPKQRHALRFDLL
                     DAENFYPPINRSQDFIVIEINLMQGRSEQLKKRLIKGLFSALHTKVGIVPMDVEITIK
                     EQPEHCWGFRGMTGNEATDLEYQAHR"
     gene            complement(5912..6502)
                     /locus_tag="PXA33_001006"
     CDS             complement(5912..6502)
                     /locus_tag="PXA33_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HDL5120348.1"
                     /translation="MANILFIDNFDSFTYNLVDQFRELGHQVTIFRNDYPLEDFLAKA
                     ETTENCLVALSPGPGNPAEAGNLLEIIRRLKDKVPMIGICLGHQALIEAFGGKVVHTG
                     TVLHGKVSRIEHDNQAMFSGINNPMLVARYHSLMGDELPAEFIVNAKFEHIVMAIRHK
                     TLPICGFQFHPESILTVQGTKLLKQSVEWLLGEKND"
     gene            complement(6512..8068)
                     /gene="trpE"
                     /locus_tag="PXA33_001007"
     CDS             complement(6512..8068)
                     /gene="trpE"
                     /locus_tag="PXA33_001007"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HDL5120349.1"
                     /translation="MPQLTITQTELPYFADTTRVYYALCGERPHTLLLDSAEIQSKNS
                     VKSLLFAKSAVHIFCEDQTVTFHALTPNGEAVLPLIANALSLLNPAPTCKFLDKNDRL
                     QAIFPTISNQLDEDSKLKTTTVFDGLRTVSQLFADSTSPIYLGGLFSYDLVANFIPME
                     NISLQDDGLSCPDYTFYLAEQLVTIDHQTETAVLNTFCFEPNEQTNLNQQAVEFTQKL
                     ANLSHIELPLANQTATSEATANIEDEDFKAIIRKLKHHINIGDVFQIVPSRRFSLPCP
                     NTLASYKQLKINNPSPYMFYMQGENFTLFGASPESALKYSQANRQLEIYPIAGSRPRG
                     FDEQGNIDPELDSRLELELRLDQKELAEHLMLVDLARNDVARVSESGTRRVVDLMQID
                     RYSQIMHLVSRVIGTLRKDLDALHAYQACMNMGTLTGAPKIKAMQLIYQVEQQKRHSY
                     GGAVGYLTSDGNLDTCIVIRSAFVQNGIAYVQAGCGEVLDSDPQLEADETRHKARAVL
                     KAIGQVNAKA"
     gene            8210..8827
                     /locus_tag="PXA33_001008"
     CDS             8210..8827
                     /locus_tag="PXA33_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014990994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HDL5120350.1"
                     /translation="MTQFFYIHPDNPQQRLINQAVEIIRNGGVIVYPTDSGYALGCAM
                     GDKHAMDRIVAIRKLPENHNFTLVCSDLSELSIYALVNNQAYRLIKNNTPNPYTFILP
                     ATKELPRRLMTKRKTVGIRVPDNAIALALISALGEPILSCSLMLPETEMTESDPDEIR
                     DHLQNRVDLIIHGGYLGQQPTTVVDLTDDVPVIIREGSGDPTSFN"
     gene            8973..10040
                     /gene="rluB"
                     /locus_tag="PXA33_001009"
     CDS             8973..10040
                     /gene="rluB"
                     /locus_tag="PXA33_001009"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HDL5120351.1"
                     /translation="MTTFQKKSTATFRADKRGESRPSFKKEEQKSERKFGKTSEKPPR
                     PTSKPLTQRIAKKVEKTTASEVASQEKKAKVVGEKLQKILARAGQGSRRELEEIIAAG
                     RVSVDGKMATLGDRVQVSSSTKIRIDGHLVNLMPAQKEICRVLMYYKPEGELCTRSDP
                     EGRSTVFDRLPRLSGARWIAVGRLDINTSGLLLFTTDGELANRLMHPSREVEREYSVR
                     VFGNIDDAMLQRLRKGVQLEDGPANFKQIKVVGGTGLNQWFDVTLTEGRNREVRRLWE
                     SQGVEVSRLIRIRYGNIKLDKGLPRGGWEEMGLEQVNYLRELVGLPPETETKVEVGVN
                     RRRTNIRQIRKAVKQHQKYRG"
     gene            10106..11878
                     /locus_tag="PXA33_001010"
     CDS             10106..11878
                     /locus_tag="PXA33_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HDL5120352.1"
                     /translation="MSWDIVATLAVTGIAVFLFATEKMRMDVVAILVLCSLVLLGQVD
                     TKSALSGFSNSATVTVTAMFVLAAGLQNSGALDSVGSLLAKVKSPWLFLLVLCGVNAA
                     VSPFVNNTAVVAVLIPIVIAAAQNIKMAPSKALIPLSFSSQMAGVCTLIGTSTNLLVN
                     AIAQKQGHSGFGMFEFAPLGIIFLVVGVAYLLVTSRFLLPESHLQLEDGEGFGKYVSE
                     LKVNKDSVLIGKSTFESGLNEEFNLFTIGVLRDGERLSTPSHQVLQKHDILLLRGESE
                     NLAKVREKYGLHHVVYGRRENDEDNLDEDLMVAEVMISPISRWIGGTIPILQQRWNKN
                     ATVLGIQRQSKVIRERLRTTAFKMGDILLLTLPKEDMETLRQDKDFIVLSSDLVKTEE
                     SWKSKFALGVMVLVVATAALGWVPISVSALIGAVAMCVAGCLTAEEAYQSIDWKIILV
                     LAGLLPLGEAMANSGAAQFIVDNTLGKVGEFGPLVVLAVLYLLTMVLTEFMSNAGTAV
                     LLTPIAVSTAKMLGVDASPFIIAVMFAAATSFMTPVGYQTNTMVYGAGGYKFTDFIKI
                     GLPLNVIYWILGIVLIPIFFPFNP"
     gene            complement(11968..>16109)
                     /locus_tag="PXA33_001011"
     CDS             complement(11968..>16109)
                     /locus_tag="PXA33_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HDL5120353.1"
                     /translation="YRIVWNHAKRTWIVASELSRSANKSNATNTSLTTNIIKLSTLSL
                     ALSAGFASAATYSPTYGTIPDNNPQTSIAIGDGSKVNSTLVSTITGLEGNSGKVSTAL
                     GYNTTATADFSTALGGFANASFASTAVGGQATADKRSVAVGYNATAMGLREVFIGDHA
                     GLNHSNSTEYNIGIGYSASSNVTGNNTISIGNTAGDGTSGSHNIAIGTYANAKLAGPT
                     TNVTSDDNIAIGNSALANGVNNYKTTAATGETVIGKATAVGSHANATGIVSSAYGAEA
                     NATAIHSTAIGAKSQANGDNSTAIGYEAKTSGHGATSLGYYANATANLTTAVGTNAGA
                     TSDYASAFGREANASGGSATALGNRATASGAASVALGVSAKATNQRTIAIGESSNASA
                     FNATAIGRNATAEHTDSIALGSNSVTAIAIPTTNATVNGITYSDFAGTNPIATVSIGA
                     EGKERTITNVAAGRISLSSTDTINGSQLYLTQQAIGNVAATTANILGGGAAVTENGNI
                     TFPTYALVNGTPDADKEGKQGRYTTVSAALSALNTAVISPLTFAGDTGTNFERHLGST
                     VKIKGGSTGILTENNIGVVADGNSTLTIKLAEKVNLGANGSLTTGDTVVNNTGITIAN
                     GVADKPVSLTKSGLDNGGNKIANVAAGDVDTDAVNVSQLKQAISKFATHYVSISDDGI
                     QRANYDNSGSSGVNPMAIGVATSANGELATALGSEAEANGERTTAVGPRATADGMNAT
                     SIGYNANANATNALAVGSAANANADTSTAIGTASTATATRATALGSKSEATGENSTAV
                     GYEASSIGADSLAAGYNANASGTQSTALGNSANAGGIWSTSVGRNANAAGSSAIALGN
                     SANAAGVASIALGVSSQATTTAAVALGQNAKATHQGSVALGTNSETVATVATKSATLN
                     GNTYTFAGTTPSSTVSIGSVGNERTLTNVAAGRILDSSTDAINGSQLYAAYTEIDGLN
                     TKVNELSNGALTFVDDAGTEIVRKLGTSLNVKGGADATILTDNNIGVVATDANTLTVK
                     LAKDIDLTPAGSVAVGNSKLNNNGLTINNGPSVTMTGVDAGKLKITNVADGDISPISA
                     DAVNGSQLYDTANTIATALGGNSSVNANGAVSAPSYTVVDGAPTNEVSKTVNNVGSAI
                     TALNDAVTSPLTFAGDTGTPSQRKLGSTVTVKGGVSNESQLTDNNIGVISNGNGSLTV
                     KLAKDIKVNSVTAQTVTANIAVADTVKTGDTTIDTNGLTIVGGPSITKTGINAAGTKV
                     TNVKAGTEDTDAVNFSQLKATEKNINNKINNIDSKVNKVDKRLRAGIAGATATAGLPQ
                     AYLPGKSMLATAGDTYRNEAAIAVGYSRISDNGKVIYKLTGNSNTRGDFGGSIGMGYQ
                     W"
BASE COUNT         4430 a         3286 c         3457 g         4936 t
ORIGIN      
        1 tttagatgag tggtactcag cttcagataa agagcgaaga gaatttatcg caaaacattt
       61 tgcagaagaa acagtaagaa gtgatagaaa tacaccgtat gattatgcta tgtgcactgg
      121 tagaatgttt gctaaacaac tgactcgaga tgaagtcaaa gactaccctt tggcaaacac
      181 taagccgcaa tatttaaccc gtgatagacg gattgagatt atgtctaatt tacctcgtga
      241 aatattacaa aattgtgcta aatacataca gtaattttat gagtaacacc actttggaat
      301 taagtggtgt tattttaatt ttaataatgt ctctaaaatc atctccaaca taaactcaat
      361 aaagccggta gaatcatttt ggctgtctgc gagagaaagc atttgataat aacgttgttg
      421 attttcgtaa accattgttt caatcggcaa ccaagcgaaa agtgggttcc attgtgctaa
      481 aaggacattt tgccataatc gacccattct gccgtttcca tcactaaagg ggtgaatcat
      541 ttctatttca tagtgcataa ccgagctttt aatgagtgca ggtgtatcat cttgtttagc
      601 ccaagagaac aactcattaa tcaaattctc tacgaattga ggacgagccc ccatatgcac
      661 aagttgccca ctgaaattat aaatgccgac atcttttgaa cgaaaagccc ctgcgtcatt
      721 tactaagtca gcagtaagaa acttatgtgc tttgagaaaa tctttaatcg agtaaggatt
      781 atagtgcatc atttcatcgt aagcgtcata ggcatttttg acttcacgaa tatctttggg
      841 gtgtccgaga acctttttgc ccttaatgat tgcggtaact tgctcaatcg ttaatgaatt
      901 tgcctcaata gctaaagagg cttgaattga tctcaagcgg ttattttttc taagatgtaa
      961 attacgctca aaatccaatt gtagcgttcc caatttatga gaaatttcta caaccagatt
     1021 cagcatttta gtggtaactg taaaaggtgg tttataagcc attttgcaat aatcctaaaa
     1081 aattaaaccg cttgtcattg ttactccacc ttcggcttat cgccgttttc aacacagcct
     1141 ttgccgatgg tgactttttt cgcattgagg gttgggagat cgtacattgt gtcaagtaat
     1201 aagttctcga caatagaacg taacccacga gccccggttt tgcggctaat cgctttttgg
     1261 gcaattgaaa ttaatgcatc tttggtgaag tgcaattcaa cgccttccat ttcgaataag
     1321 gcttgatatt gcttgatgat cgcatttttc ggctcggtta agatttgaat cagagcttct
     1381 tcgtccaatt cttgcagcgg agtcaccacc ggtaaacgcc cgattaattc aggaattaag
     1441 ccgaatttga ctaaatcttc cggttcaact tgtttgaata gctcggttaa atcttggcga
     1501 tctttatctt ttttcaatgc cgctgcaaaa ccgataccac cttgagtgtt ggtacgggct
     1561 tccacgattt tatctaaacc ggcaaaagca ccgccgcaaa taaagagaat tttggaggtg
     1621 tccaccggaa tggtttcgcc tttcgggtgt ttgcgaccgc cttgtgggtt aatgttcgcc
     1681 accgtgcctt caagtagttt taataaggct tgttgaacac cttcgcccga tacatcacgg
     1741 gtaatggatg cactttccga tttacgggtg attttgtcga tttcatcaat aaaaacaatg
     1801 cctcgttccg cttgttcggc ttcaaaatcg catttcatta ataatttttg aatcacgttt
     1861 tcgacatctt ctcccacata gcctgcttgg gtaagggtag tagcatcagc taccgcaaaa
     1921 ggtacgttta aacggcgggc aagggtttct gccaacaagg ttttaccgct accggtcggc
     1981 ccgattaata aaatattact tttacctaac tctacgccat tagtggcttg atggtttgac
     2041 aatgcacttc ttaagcgttt gtagtggtta taaacggcca ccgaaagcac ttttttcgcg
     2101 tgttcttgac cgatgacata atcatctaaa tgagcgtgta attcgtgagg tgtcggcaca
     2161 ttggtgaaaa aactgccttc atcgtcttgg ttttcatcaa ttaacggctc ttcttcaccg
     2221 tgtaataagg cgtaggattc ctcgatacat tcattacaaa tatagccttc cgtgccttcg
     2281 actaattgat cgacttcggt acgtttttta ccgcaaaaac tacagtgggg ttctttttca
     2341 aatgccatta cgccacctca ctgcgagaag ttaatacttt gtcgattaag ccatactcag
     2401 cggcttcttc cgcagacata aaattatcac gctcggtatc ggctaacact ttttcatagc
     2461 tctgaccgct gtgttctgcc atacggcggg taagggtagc tttcaagcgt aaaatttctt
     2521 ccgcttgaat ttgaatatcg gttgcttgac ctctcgctcc acctaaaggt tggtgaatca
     2581 taatacgagc gtgcggcaga gcaaagcgtt taccttttgc cccggctgaa agcaaaaatg
     2641 cccccattga agccgcttgc cctgtgcaaa gggttgctac atcgggtttg atgaagttca
     2701 ttgtatcgta aattgccaag cctgctgtta ccacgccacc cggtgagtta atgtataaat
     2761 agatgtcttt ttccggatct tcggcttcta aaaacagaag ttgtgccaca atcaggtttg
     2821 ccatattgtc ttccacctga ccgtttaaaa aaataatacg ttcttttaat aaacgtgaat
     2881 aaatgtcgta agaacgttcg ccttttgagg tctgttccac caccattggg actaatgcca
     2941 ttttgttttc ctctgtcgta atacaaaatc tcgctattct agcaaatttt cagaaaatca
     3001 ttaacaacaa gcggtcagat tgttaaaatc ttttgcaaat gatgaggaaa ttatgacatt
     3061 aacagaaatt taccaaaaat ttgaacgttg taaatcgtgg gaagaaagat accgcttgtt
     3121 aattcagcta agtcgcttgt taccaaaacc aagtgagcaa gagttggcac aaatagcgga
     3181 aattgagggc tgtgaaagcc gtttatggtt tgaatttcaa gctgagccac gaaaagtagt
     3241 ggcatatagc gatgcccgtt taatgcaggg gattttattt attgtgagtg tagctttaac
     3301 tgaaaaaacg gcagaagagc tgaaaggctt tgacttaaaa acgatgtttg atgagctaaa
     3361 aatttcacaa aatttaacca gcacaaggtt aaatgggttg ggtcagttac agaaacatat
     3421 aatgatgaat ttgtaattca ccctcgcccc ttgtgggaga gggtgaataa aatccgcagg
     3481 attttattcg ggagagggga atataaaaaa cctctgattt ctcagaggct taaatttgtt
     3541 ggcggaacgg acgggactcg aacccgcgac cccctgcgtg acaggcaggt attctaacca
     3601 gctgaactac cgctccatac tggtgctaaa ataatagtgg tttcctcaac attgagcaaa
     3661 tagtttttgc aaaatagtgt ttaattggtt gtaaaataac cactttattt ctgcttgatc
     3721 gccttgataa acagcaacgg cggacggtgc tgtaaatctt taaattcatt gtgccaccga
     3781 ggttggcctg ccagcattgg ttcttccatt tgcacaatct caaacccgcc ttgaataagg
     3841 ttattcacaa tggttgccat tgtgcggtgg taggttttaa agggttgttg aaaccaactt
     3901 ctgtcccgtt cgccctcttc ccgataatag ttcaagcggt aagcaacttg ttgtttttct
     3961 tggtttttct cccaacgata gccttctcgg taacaggtga caatcgggtg ttcttgcgag
     4021 aaaatcagcg tgccacaagc agtcattttt tgcgaaattt ttgcaagtag ttcggaaaaa
     4081 tcagcaatat aatgaaacgc aaaagagctg gttactacat cgaaatcgcc ctcgctgatc
     4141 tcatttaatc gctccatcgg caggcagtaa agggaaaaat cgttgccttt ttgccaatga
     4201 gcagaaagat cgctttctgc ctgttgtagc attaattcag ataaatccaa ccccaccact
     4261 ttttctgcac cgagcgttaa ataatgggct aaatgaactc ctgtgccaca acctaaatcc
     4321 agcacttttt tgccggttaa gtctggcagt agagagaaca ttgttggctt ttccaccact
     4381 tcattcatac ttattggatt ttgtcgaagt tgttggtaac gctcaaagaa aatcggagtg
     4441 tcgtaaatgc tttttttcac ttaatagcct ttaaacaaat ataaaataag gcgggttaaa
     4501 acccgccact acgatatgat tagtaagtcg ataattttcg caaagtttct aacgcttttc
     4561 cactctcaag cgtttctttt actttttgca cattggcttt taaatcatct tctccaaagg
     4621 ttttcattaa taatgcgaca tttgctgcca ccgcatcaat atgagccagt ttaccttttc
     4681 cttgtaaaat agcggttact ttttcggcat tttctgcagg ttcaccgcct ttgagatctt
     4741 ctaacgaatg gcgttcaatg ccgaaatctt ccggcgttaa ggtgtaatag tcgatctgtc
     4801 cgttttgaat ttccgcaacc tgcgtttcgc cgtgaacggc aacttcatct aaacctgcac
     4861 cgtataccac aatagtgtgt tcgtaaccta aggcttttac ggtttcagca tagactttca
     4921 ctaactcagg ggagtacaca ccgagtaatt ggcgtttagc gtgagccggg tttaccaacg
     4981 gccctaaaat attgaaaatc gtgcgggttt ttaaggcttg gcgaacaggg gcggcatatt
     5041 tgaatccgct gtggtaggtt ggggcaaaca aaaagcagat gttggtttca tctaaagcaa
     5101 ggcgtgccgt ttcaggtgaa acgtcaacat taatacctaa atggcttaat acatcgcttg
     5161 agccggtttt acttgatacg ctacggttgc cgtgtttggc aattttatag cccattgtgg
     5221 cggcaacaat cgcacttgtg gtagaaatgt tgatggtatt atgcccatcg ccacctgtgc
     5281 cgacaatatc cgcaaaaggg taatcaggcg ttgagaacgg attggcattt tcaagtgcgg
     5341 cttttgcagc acctgcaatt tcatcaacgg tttcgccacg cagttttaga gcgattaagg
     5401 cagcagcaag ttgttcattt gatacattgc ctttaataac ttcactaaaa aaacggtgag
     5461 attccgcttg ggctaaggct tggttggcga ataatttatc taaaagtgct tgcatagtct
     5521 cttccttttt aattagcgat gtgcctgata ttccaaatcg gttgcctcgt tgccggtcat
     5581 tccacgaaag ccccagcaat gctctggctg ttctttaatg gtgatttcaa cgtccattgg
     5641 cacaatgccg actttggtgt gtaaggcact aaatagtcct ttaattaagc gttttttgag
     5701 ctgttcgctc cgcccttgca tcaggtttat ttcaatcaca ataaaatctt ggctacggtt
     5761 aattggggga tagaaatttt ccgcatccaa taagtcaaaa cgcaaagcgt ggcgttgttt
     5821 tggtacgcca aggctcattt gcatacagtc aaaaatcacg tctgcaatca gggctctgcg
     5881 gtctgctaag gtttgtttta atccatatac actaatcatt tttttctcct aataaccatt
     5941 ccaccgactg cttaagtaat tttgtgcctt gcacggttaa aatggattcc gggtggaact
     6001 ggaaaccgca aatcggtaag gttttgtggc gaattgccat cacaatatgt tcaaatttcg
     6061 cattaacgat aaattctgcc ggtaattcat cgcccattaa tgagtgataa cgagcgacaa
     6121 gcattggatt gttaatgcct gaaaacatcg cttggttatc gtgttcaata cgagaaactt
     6181 tgccgtgcaa tactgtgccg gtatgcacta ctttgccgcc aaaggcctca attagagctt
     6241 gatgtcctaa acaaatgcca atcatcggta ctttgtcttt cagacggcga ataatttcca
     6301 ataaattgcc tgcctcagcc ggattacccg ggccggggga aagggcaacc aaacaatttt
     6361 cggtggtttc agctttggct aaaaaatcct caagcggata atcattgcgg aaaatcgtta
     6421 cctgatgccc taattcacga aattgatcga ccaagttata ggtaaaagag tcgaaattat
     6481 caataaaaag aatatttgcc atagtttgtc cttatgcttt tgcattcacc tgcccaatcg
     6541 cttttaacac cgcgcgtgct ttatggcgtg tttcatctgc ctctaattgt ggatcggaat
     6601 ccagcacctc accacaacca gcttgcacat aggcaatgcc attttgaaca aaggcggaac
     6661 ggatcacgat acaggtatct aaattgccgt ctgaggttaa atagcccacc gcaccgccgt
     6721 agctatggcg tttttgttgc tcgacttggt agatcagttg cattgcctta attttcggag
     6781 caccagttag cgttcccata ttcatacaag cttgataagc gtgcagagcg tcaagatctt
     6841 ttctaagtgt gccgatcaca cgagaaacta agtgcataat ttgcgaatag cggtcaattt
     6901 gcattaaatc aaccacacgg cgagtgccgg attcgctcac acgagccaca tcattgcggg
     6961 caagatcgac taacattaaa tgttcggcaa gctctttttg gtctaaacgc agttctaatt
     7021 ccaaacggga atctaattca ggatcgatat taccttgttc atcaaaaccg cgtgggcgag
     7081 agccggcaat cgggtaaatt tccaactggc ggttggcttg cgaatatttc aacgcacttt
     7141 caggcgacgc cccaaacaga gtgaaatttt cgccctgcat atagaacata tacgggcttg
     7201 gattgttgat tttgagctgt ttatagctgg caagagtatt cgggcaaggc aagctgaaac
     7261 ggcgagaggg cacaatttgg aacacatcac cgatattaat atggtgtttg agtttgcgga
     7321 taatcgcttt gaaatcttca tcttcaatat tggcggttgc ctcactcgtt gcggtttggt
     7381 tcgctagagg cagctcaata tgtgacagat ttgcaagttt ttgcgtaaat tcgaccgctt
     7441 gttggttcaa attggtttgt tcattcggtt caaaacagaa ggtgtttagc accgcagttt
     7501 cggtttggtg gtcgattgta accagttgct ctgctaaata gaaagtgtaa tctgggcaac
     7561 ttaagccatc atcttgcagg ctgatatttt ccatcggaat aaaatttgcc accaaatcgt
     7621 agctaaataa tccgcccaaa tagataggcg atgtggaatc agcaaacagt tggctcaccg
     7681 ttcttagccc gtcaaacacg gtggtggttt tgagcttgct gtcttcatct aactggttgc
     7741 tgatggttgg gaaaatcgct tgcaagcggt cgtttttgtc taaaaatttg caagtcggag
     7801 cagggtttaa caggctcaat gcgtttgcaa tcaaaggtaa aaccgcttct ccgtttgggg
     7861 ttaaagcgtg gaaagtcacg gtttgatctt cacagaaaat atgcactgcc gatttcgcaa
     7921 acagcaaact ttttacactg tttttacttt gaatttccgc tgaatctaat aatagcgtat
     7981 gtgggcgttc gccgcataat gcgtaataaa cacgggtcgt atcggcaaaa tagggtaatt
     8041 ctgtttgagt aatcgttaat tgaggcatag caaattccta ttcaattttt cgtactatta
     8101 aactagtaca ttaaaaagaa gtcaagcaat tattccagat aattatgcga gcttgggcga
     8161 acttggtgta taattagccc aatttttaga aagtcgaagg cttgagacta tgacacaatt
     8221 tttttatatc caccccgaca acccacagca acgcttaatt aaccaagcgg tagagattat
     8281 tcgaaacggt ggcgtaattg tttaccctac cgattcaggc tatgctctcg gttgtgcaat
     8341 gggcgataag cacgcaatgg atagaattgt agcaattcgt aaattgccgg aaaatcacaa
     8401 ttttacctta gtttgtagtg atttatccga gctttctatc tacgcgttgg tcaataatca
     8461 agcctatcgc ttaattaaga ataatacccc gaatccttat acttttattc tgccggcaac
     8521 taaggaactt cctcgcaggc tgatgacaaa gcgtaaaaca gtggggattc gtgtgccgga
     8581 taatgcgatc gcattggcgt taatttccgc cttaggtgag ccgattcttt cttgctcgtt
     8641 aatgttgccg gagacggaaa tgaccgagtc agaccctgat gaaattcggg atcatttgca
     8701 aaatcgggtg gatttaatta tccacggtgg ttatttaggg cagcagccta caacggtagt
     8761 tgacttaacc gatgacgtgc cggttattat tcgagaagga tcgggcgatc caacatcgtt
     8821 taattaaatt gatgttgtat gattaatata tagaattatg cgtagggggg ctgtgccagt
     8881 ccgtattaat atcgttattt ggacaggcac aggacctgtc cctacaatga aaatcaaatt
     8941 cagacgcttg tgaaagcgac aaaaggaacc ttatgacaac ttttcaaaaa aaatcaactg
     9001 cgactttccg tgcagacaaa cgtggtgaaa gccgtccctc atttaaaaaa gaagagcaaa
     9061 aatctgaacg caaatttggt aaaacgtcag aaaaaccacc acgtccaact tctaagccat
     9121 taacgcaaag aattgcaaaa aaagtggaaa aaacgaccgc tagtgaagtg gcatctcaag
     9181 agaaaaaagc caaagtagta ggtgaaaagc tacaaaaaat cttagcgcga gcagggcaag
     9241 gttctcgccg tgaattagaa gagattatcg ctgctggacg agtgagcgtg gacggtaaaa
     9301 tggctactct tggtgatcgt gtgcaggtta gctcatcgac taaaatccgc attgacggac
     9361 atttagttaa tttaatgccg gcacaaaaag agatctgccg tgtattaatg tattacaaac
     9421 cggaagggga actttgcacc cgttccgatc cggaaggacg atccaccgta tttgaccgct
     9481 tgcctcgttt aagtggcgct cgttggattg cggtcgggcg gttagatatt aacacctcag
     9541 gcttattatt gttcactacc gatggcgaat tagccaaccg tttaatgcac ccaagccgtg
     9601 aagtcgaacg tgaatattcg gtgcgtgtgt tcggcaacat tgacgatgcg atgttacaac
     9661 gcttacgcaa aggcgttcag ttggaagacg gaccggcaaa ctttaaacaa attaaggttg
     9721 tgggtggcac aggcttgaac caatggtttg atgtgacttt aaccgaaggg cgtaaccgtg
     9781 aggttcgccg cctgtgggaa tcgcaagggg tagaagtcag ccgtttgatt cgtatccgtt
     9841 acggcaatat caaattagat aaaggcttac ctcgtggcgg ctgggaagaa atgggcttag
     9901 aacaagtcaa ttacttgcgt gagctggtag ggttaccgcc ggagactgaa actaaagtgg
     9961 aagtgggggt aaaccgccgc cgaaccaata tccgtcaaat tcgtaaagcg gtaaaacaac
    10021 atcaaaaata tcgtggctaa acaccatctt tggggctgca ttagttctgc taatgcagct
    10081 attttctttc aaaaaaggag catttatgtc ttgggatatt gttgcaacat tggcggtcac
    10141 tgggattgcc gtatttttat ttgcaactga aaaaatgcga atggacgtag ttgcgatttt
    10201 agtgctgtgt tcattagtat tattagggca ggttgatacc aaatctgctc tgagtggatt
    10261 ttcaaactca gctactgtaa ccgtaactgc aatgtttgta ttagctgctg gtttacaaaa
    10321 tagtggtgct ttagatagtg taggcagttt acttgctaag gtaaaatctc cttggttatt
    10381 cttgcttgta ctctgtggcg taaatgctgc tgtttctcct tttgtgaata atacggcagt
    10441 ggtcgcagta ttaattccga ttgtgattgc cgcagcacag aatataaaaa tggctccttc
    10501 caaagccctt attccgttat cattttcttc tcaaatggca ggtgtttgta cactgatcgg
    10561 cacttccact aatctattag ttaatgcaat cgctcaaaag caagggcatt ccggtttcgg
    10621 tatgtttgaa tttgcccctt tggggattat ttttttagtg gttggggttg cctatttatt
    10681 agtgaccagc cgtttcttat tacccgaatc acatttgcaa cttgaagatg gtgaggggtt
    10741 tggaaaatat gtttctgaac ttaaagtaaa taaagactct gtattaattg gcaaaagtac
    10801 atttgaatca ggattaaatg aagaatttaa tctatttact attggtgttt tgcgtgatgg
    10861 ggaaagatta tcgacaccga gtcatcaagt attgcaaaaa catgatattt tattgttgcg
    10921 gggtgaatcg gaaaatttag caaaagtacg agaaaaatat ggattgcacc acgttgtgta
    10981 tggacgccgt gagaatgatg aagataatct ggatgaagac ttgatggtgg ctgaagtgat
    11041 gatttctcct atttctcgtt ggataggagg gactattcct attttacaac agcgttggaa
    11101 taaaaatgca acggtgttag gcattcaacg ccaaagtaaa gtaattcgtg agcgtttacg
    11161 caccactgct tttaaaatgg gggatatttt attactcaca ttaccaaaag aggatatgga
    11221 gacgttacgc caagataaag actttattgt gttatcttct gatttagtca aaacggagga
    11281 atcgtggaaa agtaaatttg ctttaggggt aatggttttg gtggtggcaa cagcagcctt
    11341 aggctgggtt cctatttcag tgagtgcctt aattggtgca gttgcgatgt gtgttgctgg
    11401 ttgtttaacg gctgaagaag cctatcaatc tattgactgg aaaatcattt tagtcttagc
    11461 tggattactg ccattaggtg aagcgatggc aaatagtggg gcggcgcaat ttattgttga
    11521 taacacgctt ggtaaagtag gggagttcgg gcctttagtg gtgttagctg tattatattt
    11581 actgacaatg gtgttaaccg aatttatgag taatgcaggg actgccgtgt tgcttacgcc
    11641 tattgcggtt tctaccgcta aaatgttagg ggtagatgct tccccattca ttattgccgt
    11701 aatgtttgcg gctgcgacca gctttatgac ccctgttggc tatcaaacca acacgatggt
    11761 atacggtgca ggtggctata aattcacaga ttttattaaa attggtttac cgctaaatgt
    11821 aatctattgg attttaggga tagtattaat tccgattttc ttccctttta atccataatt
    11881 gatagataat tatagaaaaa aggtttacag atcagaaact ctgtaaacct tttttattca
    11941 gcaataaatc aattttctat tggaaaatta ccattgataa cccatgccaa tactaccacc
    12001 aaagtcgcct ctggtattgc tgttaccagt taatttatag ataaccttcc cgttatctga
    12061 aatccgagaa taacctactg cgattgcagc ttcattacgg tatgtatctc cagccgttgc
    12121 taacatactt ttacctggta gatatgcttg cggtaaacct gcggttgcag ttgcaccagc
    12181 aataccagcg cgtaaacgtt tatcaacttt gtttactttt gaatcaatat tattgatctt
    12241 attattgata tttttttctg ttgcttttaa ttggctaaag ttaactgcat cagtatcttc
    12301 agttcctgct tttacgttag tcacttttgt tcctgctgca ttaatacctg ttttagttat
    12361 actcggtccc cctacaatag ttaatccatt agtatcaatg gttgtatcac cagtcttaac
    12421 agtatcggct acagcaatgt tagctgttac tgtttgagct gtgacagaat taactttaat
    12481 atctttagct aatttaaccg ttaatgaacc attaccattt gaaataaccc cgatattatt
    12541 atcagttagt tgtgattcgt tagatacccc accttttact gtaacagtag aacctaattt
    12601 acgttgagat ggcgtgccgg tatcacctgc aaatgttagt gggctggtta ctgcatcatt
    12661 taacgcagta attgcagagc ctacattatt gaccgtttta ctaacttcat ttgtaggagc
    12721 gccatcaacc acagtatagc ttggggctga aacagcacca tttgcattta cacttgaatt
    12781 accacctaaa gcggtagcaa tagtatttgc cgtatcgtaa agttggctac cattaactgc
    12841 atctgctgag ataggtgaaa tatcaccatc agctacatta gttattttta atttgccagc
    12901 atcgacacct gtcattgtga cacttgggcc attattaatg gttaagccgt tgttatttag
    12961 ttttgaatta ccaacagcta cggagccggc aggagttaaa tcaatgtctt tcgctaattt
    13021 aactgttaat gtgttagcat ctgttgccac tacaccgata ttattatcag tcaatatggt
    13081 tgcatcggcc ccacctttaa cattcagaga agtacctaat ttacgaacaa tttcagtacc
    13141 agcatcatct acaaaagtaa gtgctccatt acttaactca ttcactttcg tgtttaaacc
    13201 atcaatttcg gtataagcag cataaagttg tgaaccgttg atagcatccg ttgatgagtc
    13261 caaaatacga cctgctgcaa cattcgttaa agtacgttca ttgcctacag aaccaatact
    13321 tacagttgag cttggcgtag tacctgcaaa tgtgtatgta ttgccattta gcgttgcact
    13381 ttttgttgca actgtggcta ctgtttcaga gttagtacct aatgcaacag aaccttggtg
    13441 tgttgctttt gcattttggc caagagccac tgcagcagtt gttgttgctt gtgatgaaac
    13501 acctaatgca atagaagcta caccggctgc atttgcacta ttacctaaag caatcgcaga
    13561 gctacctgca gcattggcat ttctacccac agaagttgac catattccac ctgcattagc
    13621 agagttacct aaagcggttg actgtgtacc cgaagcattt gcgttatagc ctgcagcaag
    13681 agaatctgca ccgattgaac ttgcttcata acctacagcg gttgaatttt caccggtagc
    13741 ttcagattta gagcctaatg cagttgcacg ggtagctgtc gctgttgaag ctgtaccaat
    13801 agctgttgag gtatcagcat ttgcattggc tgcagaacca accgcgaggg catttgttgc
    13861 atttgcattc gcattgtagc ctatagaagt ggcattcata ccatcagctg ttgcgcgagg
    13921 accaactgca gttgtacgtt caccgtttgc ttctgcttca gagcctaatg cggttgccag
    13981 ctcaccatta gcacttgttg caacgccgat agccattgga ttaacaccgc tagagcctga
    14041 attatcatag ttagcacgtt gaatgccatc atctgaaata ctaacataat gagttgcaaa
    14101 tttgctgata gcttgtttga gttgactgac attgaccgca tcagtatcta catcgcctgc
    14161 ggcaacattg gcgattttat taccgccatt atctaagcca gatttagtaa gacttaccgg
    14221 tttgtctgca acgccgttag cgatggtgat acctgtattg ttaacaacgg tatcacctgt
    14281 tgttaatgaa ccattagcgc ctaagttaac tttttctgct aattttatgg ttaaagtact
    14341 attgccatca gctacaacac caatattatt ctcggttaat atgcctgtag atccgccttt
    14401 gatcttgact gttgaaccta gatggcgctc aaaattagtt cctgtatcac cggcaaaggt
    14461 taacggactg atcaccgctg tgtttaatgc gcttagtgct gcagaaacag tagtgtatct
    14521 cccttgtttg ccttctttat ctgcatctgg tgtgccattc actaaagcgt aggttgggaa
    14581 agtgatattt ccattttcag taacagctgc cccaccacct aagatatttg cggttgttgc
    14641 agcaacatta cctatagctt gttgtgttaa atataattgt gaaccgttta tggtatctgt
    14701 agagcttaaa gatatacgtc ctgcagcaac attggtgata gttctctctt tgccttccgc
    14761 cccaatactt actgtagcaa ttggatttgt tccagcaaag tcactatatg taataccatt
    14821 tacagttgca tttgtagttg gtatggctat agctgtaact gagttgctac ctagcgctat
    14881 cgaatcggta tgttcagcag tggcatttcg acctatagcg gttgcattaa aggcggaagc
    14941 atttgatgat tctccgattg caattgtgcg ttgatttgtt gctttagcgc ttacccctag
    15001 tgcaacagaa gccgcgcctg atgcagtagc acgattgcct aaagcggttg cagaacctcc
    15061 acttgcattg gcttcacgac caaatgcaga agcataatcg ctggttgcac ccgcattagt
    15121 accaacagca gtagttaaat ttgctgttgc atttgcataa taacctaatg aggttgcacc
    15181 gtggcctgaa gtcttcgctt cataaccaat tgctgttgag ttatcgccat ttgcttgaga
    15241 ttttgcacca attgctgtgg agtgaatagc tgttgcgtta gcttcagcac cataggcgga
    15301 gctaacaata ccagttgcat tggcatgact acctacagca gttgcttttc ctattaccgt
    15361 ttctcctgtt gctgctgtag ttttgtaatt attaacacca tttgcaagag cggaattacc
    15421 gatggcgata ttatcatcac tggttacatt tgtagtaggt cctgctagtt ttgcattagc
    15481 ataagtacca attgcaatat tgtggctacc actagtaccg tctccagcag tattaccaat
    15541 agaaatcgta ttgtttcctg ttacattaga gctagccgaa taaccaatac caatattgta
    15601 ttctgtgctg ttagagtgat ttaatccggc atgatcgcca ataaaaactt cacgtaagcc
    15661 cattgcggtt gcattgtacc ctactgctac ggaacgttta tcggcagtgg cttgcccacc
    15721 aacagctgtt gaagcaaaac tagcgttagc aaagcctcct aatgcagttg aaaaatcggc
    15781 tgtcgcggtt gtgttatacc ctaatgctgt agaaacttta ccgctatttc cttctaagcc
    15841 tgttattgtg cttactagtg ttgagttaac tttagatcca tcaccaattg cgatagatgt
    15901 ttgtgggtta ttatctggta tggtcccata ggttggtgaa taggttgcag cacttgcaaa
    15961 acctgcagaa agcgcaagac ttagtgtgct gagtttaata atattagtgg ttagtgatgt
    16021 atttgttgca ttagatttat tagcacttct tgataattca gaagcaacaa tccaagttcg
    16081 ttttgcatgg ttccaaacaa tgcgataaa
//
LOCUS       DANLRK010000045        15649 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.045, whole genome shotgun sequence.
ACCESSION   DANLRK010000045 DANLRK010000000
VERSION     DANLRK010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 15649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15649
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.045"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(206..1081)
                     /gene="ubiA"
                     /locus_tag="PXA33_001012"
     CDS             complement(206..1081)
                     /gene="ubiA"
                     /locus_tag="PXA33_001012"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HDL5120354.1"
                     /translation="MNNETLFSKQKWIAYAQLMRFDKPIGTLLLLHPTLWALFIAAEG
                     MPPVAILAIFTLGVIVMRAAGCVINDYADREIDGHVKRTSQRPLATGRVTTTEAKVIF
                     AILLLFAFLLDLMLNRYAFLLSFVAVFLAVIYPFMKRFTHLPQVVLGMAFGWSIPMAF
                     GAVTETLPLECWLLFFANLAWTVAYDTQYAMVDRDDDLRIGVKSTAILFAQYDNKIIA
                     LLQFITLILLGLLGVVKDYHIGYFVVLALSATLFIYQCWLTKKRERSECFKAFLNNHY
                     FGLGVFVAILVGIYF"
     gene            complement(1074..1835)
                     /locus_tag="PXA33_001013"
     CDS             complement(1074..1835)
                     /locus_tag="PXA33_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HDL5120355.1"
                     /translation="MKQANRHKKIIELVNQLGYVSTEQLVAELNVSPQTIRRDLNEMA
                     ENNLIRRHHGGAAAPSNTENSDYTHRKQFFSHEKNAIAQQVAKLIPNGASLFLDIGTT
                     SEAVALALLSHKNLKVVTNNLNAAHILMQNEDCQITVAGGGLRSDGGLIGEETVRFIN
                     QFRLDFGILGISAVDMDGSMLDYDYHEVQVKRALMECSRQVVLVTDHSKFTRTAIARL
                     GNVKEVNYLFTDQDLPLELQTHLSQSDVIVKICNE"
     gene            complement(1902..3269)
                     /gene="purB"
                     /locus_tag="PXA33_001014"
     CDS             complement(1902..3269)
                     /gene="purB"
                     /locus_tag="PXA33_001014"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HDL5120356.1"
                     /translation="MELTALTALSPIDGRYQDKVASLRPIFSEFGLLKFRVTVEVRWL
                     QKLASHTQINEVPVFSEKANDYLNQIVADFSLEDANRIKTIERTTNHDVKAVEYFLKE
                     KCEALPELQAVNEFIHFACTSEDINNTSHALMLKTAREEVLLPEWKKVIDAVAELAKC
                     YQHIPLLSRTHGQPASPTTMGKEMANVAYRLQRQYKQLENLEILAKINGAVGNYNAHL
                     SAYPEIDWHTFSQEFVESLGVTWNPYTTQIEPHDYIAEFFDCVARFNTILIDFDRDMW
                     GYIALNHFKQRTIAGEIGSSTMPHKVNPIDFENSEGNLGLANAVMAHLGQKLPISRWQ
                     RDLTDSTVLRNLGVGLGYALIAYASTLKGISKLEVNEQHLRDELNQNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDETAMREFIDKLEIPENEKNRLKEMTPASYIGYAVE
                     LVDKL"
     gene            complement(3303..3932)
                     /gene="hflD"
                     /locus_tag="PXA33_001015"
     CDS             complement(3303..3932)
                     /gene="hflD"
                     /locus_tag="PXA33_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HDL5120357.1"
                     /translation="MADYHDITLAYAGVCQAATLVQQFAHKGMADREAFKCALESLLI
                     TQPESTLSVFGDDLSHLKMGLETALAQTGGGNGKLDTEVGRYWISLLALSQKLNKNPE
                     AKQQLAQRLQQVERQLALYEDNVMADQMIANLAAIYSDIISPLGTKIHVMGMQDYLVR
                     PDIQNKIRASLLAGIRAGILWQQVGGSRWQFLFSRKKIFNQAQKFYRQI"
     gene            complement(4064..5728)
                     /gene="cydC"
                     /locus_tag="PXA33_001016"
     CDS             complement(4064..5728)
                     /gene="cydC"
                     /locus_tag="PXA33_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HDL5120358.1"
                     /translation="MKSLFPFFALYQTHFGRLLLGVVLAILGLAASIGLLSLSGWFLA
                     ASFLAGNALIFNFFYPSSGVRGLAVGRTISRYFERLVTHDATFRVLANLRASVFKKLI
                     PLSPSGLNRFRNSELLNRLVADVDTLDTLYLNLVSPFVSAVMIIAFMAIGLSFVSVPL
                     MLIICGTLLVLLAVIPTVFYRLGLKLGRNAIQSRANYRSQFIEWVQLNAEFLLFGNLN
                     QMSEKLQQTEQQWLNAQSKESRLSGLSNSLIMLSNGILTCVVIYLVSTSINVPTAKYP
                     EALIALVIFCVMASAEILSPIGIAFLHLGQVITAAERISEITEQQPNVTFGSKAEWQN
                     LSQNQPLVRCENVSFAYYGEQNVLNNLSFKVLKGQKVAILGKTGSGKSTIFQLLNRNY
                     DPTSGQIWLNNCKIDEYSEPMLRSKLVTLSQRVHIFSQTLKDNLLMGNAQATDEQMQE
                     AVRKVGLGHLLETDGLNLWLGEGGRPLSGGEQRRLGLARLLLSSAELVLLDEPTEGLD
                     RETEQQILNLILTHCCDRTLIMITHRQSGLDKFDVVYRIDNGRLVG"
     gene            complement(5728..7494)
                     /gene="cydD"
                     /locus_tag="PXA33_001017"
     CDS             complement(5728..7494)
                     /gene="cydD"
                     /locus_tag="PXA33_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HDL5120359.1"
                     /translation="MEKQRQRELQKWLKSQQKVIKKYMHLNILLGSFSSLCMIGQMWL
                     IATMLDKMLIGKQSPNSFLVEIMLLFCCFAARAFFIFLRERVGFKAGQTLRLHLRRQI
                     LAKMEAVGPMSIQQKPAGSWATLMLEQVENLHNFYARYLPQQFLSLIVPLLILCFVFP
                     INWAAGAILFATMPLLPLFMILAGMKAVEANQRNIGILSRISGQFLDKLKGLETIRLF
                     GQAEKQTEQIYQSTEDFRISTMDVLKMAFLSSAVLEFFTAVSIAVTAVYFGFIFLGEL
                     DFGYYGTGVTLFIGFFCLMVAPEFYQPMRELGVFYHDKAAAISAADNIETFLKEKVKT
                     QGGNLQENVENQPLVIQAKDCVIFSPQGKALTQALNFELNAHQHIALVGQSGAGKSSL
                     MNMLLGFLPYEGSVTINGTELRDLNLSQWRAKLAWVGQNPQLMHGSLKENILIGNPNA
                     TDSELAKALQLSKADEFVARLGLEHQVQDSNIGISGGQAQRIAIARALLRPYELLLLD
                     EPTASLDMDSEQQVLAALHNLSREQTTLMITHRVEDLTQCDEIWVMKQGQIIQKGRFT
                     ELEHTGFFAELIHNELVHQGEI"
     gene            complement(7609..11706)
                     /locus_tag="PXA33_001018"
     CDS             complement(7609..11706)
                     /locus_tag="PXA33_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB"
                     /protein_id="HDL5120360.1"
                     /translation="MTDENKQTATNQTDSAQEPQATQPKKSKWRFLKHGFCLTFILLL
                     LAILFLCTGYGQRKVLSWVDKFVEPLQLGQVQGSLQDGLQLSDARFVMDGVNVTVGQA
                     DLHIGFSCLLHYEMCLNNLSLKDTEVLIDTTKLPPSQPKEETEPFTELNLPLGISAQN
                     INLENIKVKVDDMDIHLNHFHTALSGKGRAITVHPTQLEGLNLLLALKLDETDTEQAV
                     DFAQKNAKTEALSATNPQEIADKVIQEAVEKTSEKVVEGFHQLQRKTVAQAEQVDNKV
                     DWAAIKAQLAQPLLDKNIRFSLPLDLNIEQIDIANVAIAQKAKDEKGNLIEPISLLNV
                     ASLQLQAQAKDQHINLKSLDFKSDQGDLTASGSLSLQDNYPLSWTLSGHEAKTAKVKL
                     PFNQIEASLSGELYDKTSLKVKTAGAISASLEGNVALAQAKTPFDLSLKSESMRYPFA
                     PQQNGDVLSLENIAMRLNGNLLDYALEAKVSAKGMGVPASSASLTGKGELTHFNIQDL
                     SLNTLEGTAKIDGLVDWSEGVAWRSNLKLQHINTKSLLADWPAVLSGSLSSEGYAGRG
                     NSASGWKADVSNIDIKGSLLQKNLHLSGNLKADHQQLLDVPGLSLVYGENKIDLKGVL
                     GEKSDFYADIKAPNLQGLVPNLKASVNGNAKLSGKVSEPNIDLDLVASNVGYEQFKLQ
                     NLTAKGKITTEKTIQGDLTLGLRQFAYNEIKVGNATLLLKGNEENHSLKLSAKGNPVG
                     ADLQISGKFDRLKEIWEGQLSQVSIQSTEFGQFQADKAVNVKYDNKAINANISAHCWH
                     NRKINLCFPTAFNAGVEGKVPFEIRNFDLAVLQQFLEKNSQLSGIVNAKGDAAWFKNK
                     QPQVNLDLTSNAIKFIQKMEGGKSFPLTVSPLKIDLKMSDNNLTLKSDLRVENNGRLS
                     TDLVMKDLSKARALSGNIHLDQLSLKLIRPLLERGDSVDGNVNARLTVSGTATSPLLH
                     GNLNVTELKARSVTMPFDITGGNLAMNFHGATSTLSGKLQTKQSELRLEGDADWRDLN
                     AWKTRVHAQANRFQVDIPNIAKVAVSPDIQVTATPTLLTLSGNVDIPWARIEVEQLPE
                     SAVSVSGDEVIMDGSVKNKVPFSQRNIPAQTSGGMAINADIKINIGNDVKLKAYGLNS
                     NVNGLLSVRQGKQGLGLYGQVHLREGRFAAYGQDLLIRKGDISFAGSPSQPTLDIEAI
                     RNPEAMEDPTITAGVRVTGLADNPTVKVFSDPAMSQNEALSYILTGRSLESSGDTSSS
                     NAVAAALLSMSLSKSSKLVGDVGSTFGLKDLSVTTAGIGDNTKVEVSASLAPKFRVKY
                     GVGIFAPLTELTLRYNLTPRLYLQWVSSINQAVDLMYRFEFDELF"
     gene            complement(11787..13622)
                     /locus_tag="PXA33_001019"
     CDS             complement(11787..13622)
                     /locus_tag="PXA33_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor"
                     /protein_id="HDL5120361.1"
                     /translation="MNTKLHFLTFCCLVAFASMAQANQEQRLSDVDRERVENLQEKLA
                     EDSDDEPEIEPLVTLEVKGIQGKDAKKNVELYLDQIANEYADGSERYQYLVQTTVDKA
                     LRALGYYNSRYQFVQLPQEGKKPLLVLNVELDKETVKIDETDIVLQGDAKTDEDFAHL
                     VSKAPKQGSRLNHKTYDNFKGDIESTAFSKGYFDGHWLYHRLEVYPTDHKADWRLGYD
                     SGKRYHYGQIRFVDSQIKEEYLQNILKIHSGEPYLANDLSILTQDYLSSNWFSSVMVE
                     PHLNEQDKLVDLNVLFQPKKRNEVEIGIGYETEVGPRLQLNWKKPWLNSRGHSIESRT
                     YVSKPEQTFEFGYNIPLREDPLHYYYQLSGTLENEDQNDTKSTAATLGLQRFWTHETG
                     WSFSVGLKARYDSFTQANDRFKTLLVYPTASLNRTRTDGKPYPLWGDTQKLTVNWGSR
                     AVGSDVNFYSYKASTSWARTYFDNHRFYLRAELGYLKANQFERIPPALRYFAGGDMSV
                     RGFGYKDISPRDPSNGKRVGGSHLATGTVEYQYQVYPNWWGAIFYDAGLASNKFKTQD
                     LYYGTGLGVRWASPIGAIKFDVATPVKSPDNSKGIQIYIGLGSDL"
     gene            complement(13634..14479)
                     /locus_tag="PXA33_001020"
     CDS             complement(13634..14479)
                     /locus_tag="PXA33_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HDL5120362.1"
                     /translation="MFKQINKRLNFNIFLQMFVLFTPLVQAAQMAIVIDDVGYRIKED
                     REILALPKAVSVAIIPVAPYATERAKDAYNQKRDILIHLPMEPKSKQPIEEGGIHIGD
                     NEEKIRKLIHTSRGQVPYAIGLNNHMGSGATSDSATMQHLLKVLKENTLFFLDSKTIG
                     SSVAAKTARQFGINTLERDIFLDDSDLLADVQKQFAHAINHARKNGVAVVIGHPRKNT
                     ISVLKQNLAQLPQDIELVSVGNLWRNEKTVPDKPFIMLFEQEPALSSVPPYDSIPLLR
                     GIPKE"
     gene            complement(14480..15643)
                     /locus_tag="PXA33_001021"
     CDS             complement(14480..15643)
                     /locus_tag="PXA33_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HDL5120363.1"
                     /translation="MLVKRINSYCWTAIFSGVISFSTPFSFANNLNQIQQKIQQQTSK
                     INEQKQKRNALQSTLKNQEVEMGKVLDKLQKTEMSLTEVRQTIKATETEIARLEKLEK
                     QQKEKLKEQLDSAYRSGVNPSVLERLMSEEAKNAERMTAYYQHMNNIRIDAIVEIRKT
                     QADLKARRDELKGQQKDHQTQLNEQKKQEKDLKKVQSQRESTLRLIDKTLEQDQSRLE
                     NLRSNEQALKQQLEQAQREAQQAEKREQQREKQAKSGQSKTTENVERTPIKAGRYTMP
                     VSGNIITPFGNNWHGVVIGASAGTSVRAMASGRVIMAQWLAGYGNMVAIDHGNGDISL
                     YGYNQSISVSKGSRVQGGQVIAKVGNSGGQSRSALYFGVTRKGTPINPLNLVK"
BASE COUNT         4377 a         3519 c         2932 g         4821 t
ORIGIN      
        1 tggcattgta atgtcccttt tttgaatgtc tactacaaaa tgggatattt tacaaaccga
       61 ttgactaatg aagcaagaat ataattaact acaactggat atattttgca aatttcccga
      121 taaatttcac cgcttgtaga taaaaaccgt agataaaaac catagataaa aaataggagc
      181 taaacatttt tagcccctac cgtcatcaga aataaatccc gactaaaatc gccacgaaca
      241 cacctaagcc gaaatagtgg ttatttagaa acgctttaaa gcattcgcta cgttctcttt
      301 ttttggtcag ccaacattgg tagataaaca gcgtggcact taaggctaat acgacaaaat
      361 agccgatgtg ataatctttt accacgccca ataaccccaa taaaatcaag gtgataaact
      421 gtaataacgc aataatttta ttatcgtatt gagcgaataa aattgcagtg gatttaactc
      481 caattcgtaa atcatcgtca cgatcaacca ttgcatattg ggtgtcgtag gcgactgtcc
      541 acgctagatt ggcaaaaaac agcagccagc attcaagtgg aagagtttcc gttaccgcac
      601 caaatgccat cggaatcgac caaccgaatg ccattccaag cactacttgt ggcaagtggg
      661 taaaacgctt cataaaggga taaatcaccg ccagaaaaac tgccacaaag gaaagcagaa
      721 acgcatagcg gtttagcatt aaatcgagca gaaaagcgaa caacagcaag atggcaaaaa
      781 tgacttttgc ctcagtggtg gtcactctgc cggtagctaa tgggcgttga gaggtgcgtt
      841 taacgtgtcc gtcaatttct ctatcggcat aatcattaat cacacaacct gccgcacgca
      901 taacgattac cccaagggta aaaatggcta aaatcgcaac aggcggcatt ccctctgcgg
      961 caataaataa cgcccatagt gtggggtgta ataataataa cgtgccgatg ggtttatcga
     1021 aacgcattaa ctgggcgtag gcaatccatt tttgtttaga aaaaagagtt tcgttattca
     1081 ttacaaattt tgacgataac gtccgattgg cttaaatgag tctgtaattc taacggtaaa
     1141 tcttgatctg taaacaaata atttacttct ttaacatttc ctaaacgtgc aatagctgta
     1201 cgggtaaact ttgaatgatc ggtcactaaa accacttgtc gggaacattc cattaatgca
     1261 cgctttacct gcacttcgtg gtaatcgtaa tcgagcatag agccgtccat atccaccgca
     1321 ctaatgccta aaatcccaaa gtccaaacgg aattggttaa taaagcgaac cgtttcttct
     1381 ccgattagcc cgccatcact tcttagccct ccgcctgcta ccgtaatttg gcaatcttca
     1441 ttttgcatca gaatatgagc cgcatttaaa ttattggtaa ctacttttaa atttttatgc
     1501 gaaagtaagg ctaatgccac tgcttctgaa gtcgtgccaa tatctaaaaa taatgatgct
     1561 ccatttggaa tcagttttgc cacctgttgg gcaatcgcat ttttctcgtg tgagaaaaat
     1621 tgcttgcggt gggtgtaatc actattctcc gtattggaag gagcggctgc cccaccgtga
     1681 tgacggcgaa tcagattatt ttctgccatt tcattcagat cacggcgaat ggtttgcgga
     1741 ctcacattca attcagccac taattgctcg gtactcacat agccaagctg attaaccagc
     1801 tcaatgatct ttttgtgtct gtttgcctgt ttcatatttc ctcttttgca aataatttgc
     1861 agaaaaagac cgcttgcaag cggtcttttt tcagttaaat tttacaattt atcaacaagt
     1921 tcaaccgcat agccgatata gcttgccggt gtcatctctt tcaaacgatt tttttcattt
     1981 tccggaattt ccaacttatc gataaactca cgcattgcgg tttcatcaac ccgtttgcct
     2041 cgagtcagct ctttaagttt ttcatacggt ttttcaatgc cataacgacg cataacagtt
     2101 tgaattggct ctgccaatac ttcccagttt tggttgagtt catcacgcaa gtgttgttca
     2161 ttcacttcta atttgctaat accttttaag gtggacgcat aagcaattaa cgcataacct
     2221 aaacctacac ctaaattacg caacacggtg gagtcagtta agtcacgctg ccagcgagaa
     2281 atcggcaatt tttgccccaa atgagccatt accgcatttg ccaagccaag attgccttcg
     2341 gaattttcaa aatcaatcgg gttcacttta tgtggcattg tgctagagcc gatttcaccg
     2401 gcaatagtgc gttgtttgaa gtgatttaag gcaatgtagc cccacatatc acgatcaaaa
     2461 tcaattaaaa tcgtattaaa acgagccacg caatcaaaga actccgcaat ataatcgtgc
     2521 ggttcaattt gggtggtgta tgggttccac gtcacaccta acgattccac aaattcttgg
     2581 ctgaaagtgt gccaatcaat ctccggatat gctgataaat gtgcgttata gttgcccact
     2641 gctccattga ttttagcgag aatttctaaa ttttctaact gtttatattg gcgttgtaaa
     2701 cgatatgcca catttgccat ctctttaccc attgtggtcg ggcttgccgg ctgaccgtgt
     2761 gtgcgagaaa gcaacggaat atgttggtaa catttcgcca gctctgctac agcatcaatc
     2821 acttttttcc attcaggtaa taatacttct tcacgagcgg ttttaagcat taaagcgtgc
     2881 gaagtattat tgatatcttc agaagtacaa gcaaaatgaa taaattcatt caccgcttgt
     2941 aattcaggca acgcttcaca tttctctttt aggaaatact ccaccgcttt tacatcgtgg
     3001 ttggtggtac gttcaatcgt tttaatccga ttggcatctt ctaacgaaaa atcagccaca
     3061 atttggttta ggtaatcgtt tgctttttca gaaaacactg gaacttcatt aatttgtgta
     3121 tgcgatgcca atttttgtaa ccagcgaact tccaccgtca cacggaattt taataagcca
     3181 aattcactaa aaatcggacg aaggctcgca actttgtctt gataacgtcc atcaatcggg
     3241 gataacgcag ttaaagcggt aagttccatt gttttatatt ctccaaatag gtaaatgttc
     3301 ggttagattt gacgataaaa tttctgtgct tggttaaaaa ttttcttgcg tgaaaataaa
     3361 aattgccaac ggcttccgcc gacttgctgc cacaaaatac cggctcgaat accggctagt
     3421 agcgaggctc gaattttatt ctgtatatcc gggcgaacca agtaatcttg cattcccatc
     3481 acgtgaattt tcgtacctaa cgggctgata atatcgctat aaatagcggc taaattggca
     3541 atcatttgat ctgccatcac attatcttca tacagggcta actggcgttc cacttgctgt
     3601 aaacgctggg ctaactgttg ttttgcttca gggtttttat tgagtttttg gctaagggcg
     3661 agcaggctta tccaataacg tccgacttcg gtatctaatt tgccgttacc gccgccagtt
     3721 tgagctaatg cggtttctaa gcccattttt aaatgggaaa gatcatcgcc aaatacactt
     3781 aacgttgatt ccggctgagt gattaacagg ctttctaaag cacacttaaa agcctctctg
     3841 tctgccatgc ctttatgtgc aaattgttgc actaacgtcg ccgcttggca aacgccggca
     3901 taagcaaggg taatatcgtg gtaatccgcc atctctgtaa aattcaatat gataaaacat
     3961 tagtgggaat atagcatttt ttcagcttct tttgaatggc aaaaggcatt tattctattg
     4021 aaaaataaat gccttttcat taatacgcaa tttgctgcaa aaactatcct accaatcgcc
     4081 cattatctat tcgataaacc acatcaaatt tatccagtcc gctttggcgg tgagtgatca
     4141 taattaaggt gcgatcacag cagtgagtca aaattaggtt taaaatctgc tgttcggttt
     4201 ctctgtccag tccttctgtt ggctcatcaa gtaagactaa ttcagcagaa ctcaggagca
     4261 aacgggcaag ccctaaacgg cgttgttcgc caccagaaag cggtctgccg ccctcgccta
     4321 accataaatt taagccgtct gtttcaagca aatgccctaa gcctactttt cttactgctt
     4381 cttgcatttg ttcgtctgta gcttgagcat tccccattaa taaattatct tttagggttt
     4441 ggctaaaaat atgcactcgt tggcttaagg taactaattt tgagcgtaac atcggctctg
     4501 aatattcgtc aattttacaa ttattcagcc aaatttgacc gcttgtaggg tcgtagttgc
     4561 ggtttaacag ttggaatatg gtcgattttc cgctacccgt tttacctaag attgccactt
     4621 tctgcccttt taatacctta aaggaaagat tatttagtac gttttgctcg ccgtaataag
     4681 caaagctcac gttttcacag cgtacaagcg gttgattttg gctcaaattt tgccattctg
     4741 ccttgctacc aaatgttaca ttaggctgtt gttcggtaat ttcactaatc cgctcggcgg
     4801 cggtaatcac ttgccctaaa tgcaaaaagg cgataccaat cggggaaaga atctccgccg
     4861 atgccattac acagaaaatc actaacgcaa ttaaggcttc agggtatttt gcagtgggca
     4921 cattaatcga agttgaaacc aaatagatca ccacacaagt caaaatgccg ttggataaca
     4981 ttatcaagct atttgataag ccggacagtc ggctttcttt gctttgagcg ttcagccatt
     5041 gttgttcagt ttgctgtagt ttttcgctca tttggttcaa gttgccaaag agtaaaaatt
     5101 ctgcatttaa ctgtacccac tcaataaatt ggctgcggta atttgctcgg ctttgtattg
     5161 cattacgccc taatttcaaa cccaatcgat aaaatacggt aggaataacg gcaagcaata
     5221 ccaatagtgt gccgcaaatg atcagcatta atggcacgga aacaaaagaa agcccgattg
     5281 ccataaatgc aataatcatc actgcactga caaaaggcga gactaaattc agatataggg
     5341 tgtctaacgt atccacatct gccaccaagc ggtttaacaa ttcgctgttt ctaaagcgat
     5401 ttagcccgct tggactcagt ggaatcaatt ttttaaagac gctagcacgc aaattcgcca
     5461 atacccgaaa agtcgcatcg tgggtgacta aacgttcaaa gtaacgtgaa attgtccgcc
     5521 ctactgccaa ccctctcacc cccgatgaag ggtagaaaaa gttaaaaata agcgcattgc
     5581 cggctagaaa tgaggcagct aaaaaccaac cggaaaggct gagcaaaccg atacttgccg
     5641 ctaatcctaa aattgccagc acaacaccta ataatagtcg cccaaaatgg gtttggtaga
     5701 gagcaaaaaa cgggaacaac gatttcatta gatttctccc tgatgcacta attcattatg
     5761 tattaattcc gcaaaaaagc cggtgtgttc taattcagtg aaacgacctt tctgaatgat
     5821 ttgcccttgt ttcatcaccc aaatttcatc gcattgggtt aaatcttcca ctcgatgagt
     5881 aatcattagc gttgtttgct ctcggctgag attatgcaga gcggctaaca cttgctgttc
     5941 cgaatccata tcaaggcttg cagtcggctc gtctaacagt aataattcgt acgggcgaag
     6001 caacgctcgt gcaatcgcaa tccgctgtgc ttgcccgcca gagataccga tattactgtc
     6061 ttgcacttga tgctccaacc ctaaacgtgc cacaaattca tcggcttttg aaagctgtaa
     6121 tgccttggct aattcagaat ctgtcgcatt agggttgcct attaaaatat tctctttcaa
     6181 actgccatgc attaattgcg gattttgccc cacccacgct aatttcgctc gccattggct
     6241 taaatttaaa tctcgcagtt cggtgccgtt aatcgtaacc gacccttcat aaggcaagaa
     6301 accaagtaac atattcatta atgaactttt tcctgcacca ctttgcccaa ccaaagcgat
     6361 atgttggtgt gcattcaact cgaaatttaa cgcttgggtt aaggctttgc cttgtggtga
     6421 gaagatcacg caatctttgg cttgaatcac aagcggttga ttttcaacat tttcttgcaa
     6481 attgccacct tgcgttttca ccttttcttt taagaaagtt tcgatattat ctgcggcact
     6541 aatcgctgct gccttgtcgt gataaaacac gccaagttca cgcatcggtt ggtaaaactc
     6601 aggtgccacc attagacaaa agaagccgat aaataaggtc acgcccgtac cataatagcc
     6661 aaaatccagc tcacctaaaa aaataaagcc aaaatagacc gccgtcaccg caatcgatac
     6721 cgccgtgaaa aactccaata ccgcagagga taaaaacgcc atttttagta cgtccatcgt
     6781 actgattcgg aaatcttcgg tgctttggta gatttgttca gtctgctttt ccgcttgccc
     6841 aaataagcga atggtttcta aaccttttaa tttatctaag aactgtccgc taatacggga
     6901 aagaatgccg atattacgct gatttgcctc cactgctttc attccggcta aaatcataaa
     6961 taaaggtaat aatggcattg ttgcaaataa aatagcccct gctgcccagt taatcgggaa
     7021 tacaaaacag aggatcagta atggcactat cagcgataaa aattgctgtg gcaaatagcg
     7081 tgcgtagaaa ttatgtagat tttccacctg ctcgagcatt aaggttgccc agctgcccgc
     7141 cggtttttgt tgaatgctca tcggcccgac tgcttccatt ttggctagaa tttggcggcg
     7201 aagatgtaaa cgcaaggttt gccctgcttt gaaaccgaca cgctcacgca gaaaaatgaa
     7261 gaatgctctg gcagcaaaac aacagaataa gagcataatt tcaaccagaa agctattcgg
     7321 tgactgcttt ccgataagca ttttgtctaa cattgtggca atcagccaca tctgtccaat
     7381 catacataaa ctactgaaac tccctaacag tatgtttaag tgcatatatt ttttgatgac
     7441 tttttgctga cttttaagcc atttttgtaa ttcgcgttgg cgttgttttt ccataatttt
     7501 gtcaattttt ttgttttttc aaccgcttgt aaatcgggtt tgccattcta ctataaagca
     7561 aaaaggttgt taatggcgaa acattaacaa cctttcattc agccttaact agaatagctc
     7621 gtcaaattca aagcgataca ttaaatctac cgcttggtta atacttgaga cccattgtaa
     7681 gtacaatctt ggcgttaaat tatagcgtaa ggttaattcg gtaagcggtg caaaaatgcc
     7741 cacgccatac ttcactctga atttaggggc aaggctggca cttacttcta ctttggtgtt
     7801 atcgccaata cctgcggtgg tcacgcttaa atctttgagt ccgaaagtgc taccaacatc
     7861 acccaccaat ttactgcttt ttgataagct catactgagt aatgccgctg cgactgcatt
     7921 actggagctg gtatcgccac tgctttccaa tgaacgacca gttaaaatat aggaaagtgc
     7981 ttcattttgc gacattgccg gatcagagaa tacttttacc gttggattgt ctgccaaacc
     8041 ggtcactctt accccagctg taatggtcgg atcttccatt gcctccggat tgcgtatagc
     8101 ttcaatatct aaggttggct gagacggtga gccggcaaaa ctaatatcac ctttacgaat
     8161 cagcaagtct tgaccataag cggcaaatcg tccttcacgc aaatgaactt gcccgtaaag
     8221 ccctaaacct tgtttgcctt gcctgacaga caataagcca ttcacgttac tgtttaaacc
     8281 ataggctttg agtttgacat cattaccgat gttaatctta atatctgcat taattgccat
     8341 tccacctgag gtttgagccg gaatattacg ttggctaaac ggcactttat ttttcacaga
     8401 gccgtccata atcacctcat ctccacttac actgaccgca ctttcaggta actgctcgac
     8461 ttcaattctt gcccaaggga tatctacatt tccacttaag gttaaaagag ttggcgttgc
     8521 cgtcacttga atatccgggc taactgccac ttttgcgata ttaggaatat ccacttggaa
     8581 acggttagct tgagcgtgaa cccgtgtttt ccacgcattt agatctcgcc agtctgcatc
     8641 accctccaaa cgcagttcgc tttgtttggt ttgtaattta ccgcttaaag tcgatgttgc
     8701 cccgtggaaa ttcattgcta aattcccacc tgtaatatca aacggcattg taacagagcg
     8761 agcttttaac tctgtaacgt ttagatttcc gtgcaataac ggagaggttg cggtgccgct
     8821 cacggtaaga cgagcattca cattgccatc aaccgaatcg ccccgttcaa gtagcggtcg
     8881 aatcagcttt aagcttaatt ggtcaagatg aatattgccg gataacgcac gagccttact
     8941 taaatctttc atcactaaat cagtcgaaag tctgccgtta ttttcgaccc ttaaatcaga
     9001 tttaagggtt aaattattgt cagacatttt cagatcaatt ttaagcggtg aaacggtgag
     9061 tgggaaactt tttcccccct ccattttttg aataaattta atcgcattag aggttaaatc
     9121 cagattcact tgaggctgtt tgtttttaaa ccaagctgca tcgcctttgg cattcacaat
     9181 ccctgaaagc tggctgtttt tctccaaaaa ttgttgcaat accgctaaat caaagttacg
     9241 gatttcaaaa ggcactttac cctcaacacc ggcattaaat gcagtcggga aacaaagatt
     9301 aattttccgg ttatgccaac aatgtgcgga aatattggcg ttaatggctt tattgtcata
     9361 tttcacattc actgctttat ctgcttggaa ttgaccaaac tcggtggatt gaatcgaaac
     9421 ttggcttaat tgcccttccc aaatttcttt caaacggtca aattttccag aaatttgcaa
     9481 atcagcacca accggattgc cttttgcact taatttcaag ctatgatttt cttcatttcc
     9541 ttttagcaat aaagtggcat ttcccacttt gatttcatta taggcaaatt gacgtaaccc
     9601 caaggttaaa tccccttgaa tggttttctc agtggtaatt ttgccttttg cggttaaatt
     9661 ttgcagttta aattgctcat agcctacatt tgaggccact aaatctaaat caatgttagg
     9721 ttctgaaacc ttacccgata atttggcatt accattcacg ctggctttta aattcggcac
     9781 taacccttgc aaattagggg ctttaatatc cgcatagaaa tcagattttt cgcctaacac
     9841 gccttttaaa tcgatcttat tttcaccata caccagcgat aagcccggaa catctaaaag
     9901 ttgttgatga tcggctttta aattaccgct taagtgtaga tttttctgta ataacgaacc
     9961 tttaatatca atattcgaga cgtccgcttt ccatccgcta gctgaattgc cccgtcctgc
    10021 ataaccttcg gaagataaag aaccggacaa taccgccggc cagtctgcca gtagggattt
    10081 agtgttaata tgctgaagtt tcaaatttga acgccacgca acgccctcgc tccaatccac
    10141 taaaccgtcg atcttcgccg taccttccag cgtattgaga cttaaatctt gtatattaaa
    10201 atgggttaat tcccctttgc cagttaaact agccgagctt gcaggcacgc ccattccttt
    10261 ggcactgact ttagcctcca aagcgtaatc caggagattg ccattaagcc tcatggcgat
    10321 attctctaaa ctcagcacat caccattttg ctgaggagca aacggataac gcattgactc
    10381 actcttcagc gataaatcaa agggagtttt cgcctgagcc agtgcaacat tgccctccaa
    10441 cgaggcgcta attgcaccag ccgtcttcac ttttaggctg gttttgtcat atagctcacc
    10501 ggaaagagag gcttcaattt gattgaacgg caattttact ttcgccgttt ttgcctcgtg
    10561 accacttagc gtccaactta gcggataatt atcttgtaac gataaagaac cgcttgcggt
    10621 tagatcccct tggtcgcttt tgaaatctaa actttttagg ttgatatgct gatcttttgc
    10681 ttgagcctga agctgtaaag aggcaacatt taacaatgag atcggctcaa tcaaattccc
    10741 tttttcatct ttagcttttt gagcaatcgc tacattcgca atatcaatct gttcaatatt
    10801 gagatcaaga ggcaaactaa agcgaatatt tttatccaat aacggctgtg ctaattgtgc
    10861 tttaatcgct gcccaatcca ctttattatc cacctgttcg gcctgtgcaa cagtttttct
    10921 ctgtaattgg tgaaagccct ctacgacttt ttcactggtt ttttccaccg cctcttgaat
    10981 cactttatca gcaatctctt gtggatttgt cgctgataac gcctctgttt ttgcattttt
    11041 ttgtgcaaaa tcgaccgctt gttcagtatc agtttcgtct aactttaagg ctaaaagcag
    11101 atttagcccc tctaactgtg tcgggtgaac ggtaatagcc cgccctttac cagaaagtgc
    11161 agtgtggaaa tgattaagat gaatatccat atcatcaact ttgaccttga tattctctaa
    11221 attgatattt tgagctgaaa tccccaatgg caaatttaat tcggtgaaag gttcggtctc
    11281 ttcttttggc tgagaagggg gtaatttagt cgtatcaatc aacacctctg tatctttaag
    11341 gctgagatta ttcaaacaca tttcatagtg gagcaagcag ctaaaaccaa tatgcagatc
    11401 tgcttgccca accgttacat tcacaccgtc catcacaaat cgggcatcag aaagctgtaa
    11461 cccatcttgc aggctacctt gcacttgccc aagttgtaat ggctctacga atttatctac
    11521 ccagcttaac actttacgtt gcccgtaacc tgtgcataga aagagaatgg cgagtaaaag
    11581 tagaataaac gtcagacaaa agccgtgctt caaaaaccgc cactttgatt ttttaggctg
    11641 tgttgcttgt ggctcttgtg ctgagtccgt ttgatttgtg gctgtttgtt tattttcgtc
    11701 agtcataatc gtgcatattt atgaggtgat aaaaagtaaa caagcggtat gcgaaatttt
    11761 actgaaattc gaccgcttgt tttcatttac agatcggaac ctaaaccaat ataaatttgt
    11821 attcctttgg aattatctgg tgattttacc ggcgtggcga catcaaattt aatcgccccg
    11881 ataggtgatg cccaacgcac ccctaagccc gtaccgtagt agagatcctg agttttgaat
    11941 ttattcgatg ccaagcctgc atcataaaaa attgcacccc accaattagg gtaaacctga
    12001 tactgatatt ctactgtgcc tgtggctaaa tgagagcctc cgacacgttt gccattactc
    12061 ggatcacggg gggaaatatc cttatagcca aagccccgca cactcatatc gccaccggca
    12121 aaatagcgta acgccggtgg aatacgttca aattgattgg ctttcaaata ccccaactcc
    12181 gcccttaaat agaaccgatg gttatcaaag taagttctcg cccaagatgt cgaggcttta
    12241 taactataaa agtttacatc tgaacctact gcacgactcc cccagtttac cgttaatttt
    12301 tgtgtatccc cccataatgg gtaaggcttg ccatctgttc tggttcgatt caatgatgcc
    12361 gttgggtaaa ctaataaggt tttaaatcga tcattagctt gagtaaaaga atcgtaacgg
    12421 gcttttaaac caacagaaaa tgaccaaccg gtttcgtgag tccagaaacg ttgtaagcca
    12481 agggtggcag cggttgattt tgtatcgttt tgatcttcgt tttctaaagt gccggataat
    12541 tggtaataat aatgtagcgg atcttctcta agtggaatat tataaccaaa ttcaaatgtc
    12601 tgctccggct tagaaacgta cgttcggctt tcaatactat gacctcggct atttaaccaa
    12661 ggtttcttcc agttaagctg taaccttggg ccaacttcag tttcgtagcc tatcccaatt
    12721 tccacttcat tgcgtttttt aggctgaaat aacacattta aatccactaa tttatcctgt
    12781 tcatttaaat gtggctcaac catcactgat gaaaaccaat tactggaaag gtaatcttgg
    12841 gttaaaatcg aaagatcgtt agctaaatat ggctcaccgg aatgaatttt aagaatattt
    12901 tgcaaatatt cttctttgat ttggctatca acaaaccgga tttgtccata atggtatcgt
    12961 tttccgctat catagcctaa acgccaatcc gctttatgat cggtcggata aacttccaaa
    13021 cggtgataaa gccaatgacc gtcaaaatac cctttactaa aagcggtact ttcaatatca
    13081 cctttaaaat tatcataggt tttatggttt aaacgactac cttgttttgg ggctttactg
    13141 actagatgag cgaaatcttc gtccgttttt gcatcgcctt gcaaaacaat atcggtttca
    13201 tcaattttta ccgtctcttt atctagctca acatttaata ctaagagcgg tttctttccc
    13261 tcttgaggta gctgcacaaa ttggtatcgg ctattataat agcccaaggc tctcagagct
    13321 ttatctactg tggtttgcac caaatattga taacgctctg aaccatctgc atattcattg
    13381 gcaatttgat ctaaatatag ctccacattt ttcttggcat ctttaccctg aatgcctttg
    13441 acttctaaag tgactaatgg ctctatttct ggctcatcat cactatcttc cgctaatttt
    13501 tcttgcagat tttcaactcg ctcacgatct acgtcagaca gcctttgctc ttgatttgct
    13561 tgtgccattg aagcaaaagc cactaaacag cagaatgtta aaaaatgtag ttttgtattc
    13621 attagtaacc tttttattct tttggaatgc cacgcaaaag cggaatactg tcataaggcg
    13681 gaacagaact taacgctggc tcttgctcaa agagcataat gaatggtttg tcaggcactg
    13741 ttttttcatt acgccacaag tttccaacac tcaccaattc aatatcttga ggtagttgtg
    13801 ctaaattttg cttcagtaca ctaatcgtat tcttgcgagg gtgtcctatc accaccgcta
    13861 ccccgttttt tcgtgcgtga ttaattgcat gtgcaaactg tttttgtaca tcagcaagca
    13921 gatcgctatc atctaaaaaa atgtctctct ccagcgtatt aataccaaat tgacgagccg
    13981 ttttagccgc cacactactg ccaatagttt tactgtctaa aaagaaaagc gtattttctt
    14041 ttaacacttt caacaaatgt tgcattgtag cagagtctga agttgctcca ctgcccatat
    14101 ggttatttaa accaatagca tatggtactt gaccacgaga agtatgaata agtttccgaa
    14161 ttttctcttc gttatcacca atatgaatac ccccctcttc aattggctgt ttcgatttag
    14221 gttccattgg caaatgaatc aaaatatccc gtttttggtt ataagcgtcc tttgcccgtt
    14281 ctgttgcata gggtgcaaca ggaataatcg ccacgcttac cgccttgggt aaggctaaga
    14341 tttctcgatc ttcttttatt cgatagccca catcatcaat tacaatcgcc atttgagctg
    14401 cttgtacaag cggtgtaaaa agcacaaaca tttgcaaaaa aatgttaaaa ttcaaccgct
    14461 tgttaatttg tttaaacatc tatttcacca aatttagagg attaatcggc gtgcctttac
    14521 gggtaacacc aaaatagagt gcagaacggc tttgtccccc actgttacca accttcgcaa
    14581 taacctgtcc gccttgcact cggctaccct tgctgactga aatagattgg ttatagccat
    14641 aaagggaaat atcaccattg ccgtggtcaa tcgcaaccat attgccatac cctgcaagcc
    14701 attgagccat tatgactcgc ccgctcgcca ttgctctaac cgaagtaccg gcagaagcac
    14761 caatcactac accgtgccaa ttattaccaa aaggagtgat aatattgcca gacaccggca
    14821 tcgtatatcg accagccttg atcggtgttc tctcaacatt ctcagttgtt ttggactgac
    14881 cggattttgc ctgtttttcc cgctgttgtt cacgtttttc tgcttgctga gcctctcttt
    14941 gcgcttgttc taattgttgt tttagtgcct gttcattgct gcgtaaattc tctaatcggc
    15001 tttgatcctg ctcaagcgtt ttatcgatta aacgtaaggt actttcacgc tggctttgta
    15061 ctttctttaa atctttctct tgtttctttt gttcattaag ttgcgtttga tgatcttttt
    15121 gctggccttt caattcatca cgtctggctt taagatcagc ttgggtttta cgaatttcaa
    15181 cgatcgcatc aatgcgaata ttattcatat gctgataata agcggtcatt ctttccgcat
    15241 tttttgcctc ttcagacatt aaacgctcta agaccgaagg gttcacccca gaacggtagg
    15301 cagagtccaa ctgctctttt aatttttctt tctgctgttt ttccaacttt tctaaacggg
    15361 caatttcagt ctcagtcgct ttaatggtct ggcgaacttc cgttaaagac atttcggttt
    15421 tttgtaattt atccaacacc ttccccattt ctacttcttg gttttttagg gtagattgca
    15481 acgcattacg tttttgcttt tgttcattaa tttttgaggt ttgctgttgg attttttgct
    15541 gaatctgatt aagattattt gcaaaagaaa aaggggtaga aaagcttatt accccactga
    15601 aaatagcagt ccaacagtaa gaatttattc gtttcaccag cataccttt
//
LOCUS       DANLRK010000046        15472 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.046, whole genome shotgun sequence.
ACCESSION   DANLRK010000046 DANLRK010000000
VERSION     DANLRK010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 15472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15472
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.046"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            73..963
                     /locus_tag="PXA33_001022"
     CDS             73..963
                     /locus_tag="PXA33_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL5120364.1"
                     /translation="MDKLNAISLFCKVIETQSFTQAAQQAQISLPMASKLVAQLEEHL
                     NVRLLQRTTRKITPTEAGLLYYQQCLPILNELKEADASVSNINSTLQGKITMSVPMDF
                     GSRFIAPYLGKFMEDYPNLALHIEFSDRRVDVIAEGFDLVLRIGSLADSSIVAKKIAD
                     SHLVLVASPDYLARFGEPKGLEALVERDCLLYEYHPQWQFSQQGQKMQIKPQGKIYSN
                     NGYALVQMAKAGLGIINIPKFLIKHELKNGELIPIMPFLTQEPLQISLLYPNRRYLSP
                     KVRILIDFFTQLMQEQKEYI"
     gene            complement(1013..1273)
                     /locus_tag="PXA33_001023"
     CDS             complement(1013..1273)
                     /locus_tag="PXA33_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005718542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDL5120365.1"
                     /translation="MGWIAAIIVGAIIGWIASAVMKSEGGLCKNIIIGILGSSLGRWV
                     FGDVLGIGSAYSAGDFNIVGIFFGVLGAALLIFILRKLSIIN"
     gene            complement(1353..2312)
                     /locus_tag="PXA33_001024"
     CDS             complement(1353..2312)
                     /locus_tag="PXA33_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL5120366.1"
                     /translation="MKFHKTLLVTLLSSLSVAALAQDKIQNVAITAIVEHPALDSIRK
                     GVIEELAREGFVDGKNIKIDYQSAQGSTATAAQIARKFVGDKADIIIPITTPSAQPVV
                     AATRSIPIVFSGVTDPVAAKLVKSWEPSGTNVTGISDHKAMAPQVKLIQTLVPDLKAV
                     GYVYSAGEVNSAIVLEELKQEAQKQGFTIVPVAVQRSADIGTAARSLNGKVQAIYISE
                     DNAVVSAYEALHKAALEAKIPVIAADRDTVQRGAVAAYAVNQYDIGVATGKVAARVLK
                     GEKAGTIPIQEVSKLELSINTKTAKELGITIPEVLIKEAKQTF"
     gene            complement(2509..2916)
                     /locus_tag="PXA33_001025"
     CDS             complement(2509..2916)
                     /locus_tag="PXA33_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HDL5120367.1"
                     /translation="MKPLRPYLYNAYYNWIIDSDNTPYLLVNAEFPDVDVPVEFVKEG
                     KIILNISPRSIGQYIVADEYISFSARFQGMLRDIYIPFGAMVAIYAQETGDGIMFQEE
                     AYYTEEAYQARVRAVEQPKKIKAKKTSTLKLVK"
     gene            complement(2916..3560)
                     /gene="sspA"
                     /locus_tag="PXA33_001026"
     CDS             complement(2916..3560)
                     /gene="sspA"
                     /locus_tag="PXA33_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HDL5120368.1"
                     /translation="MTSSINKRSVMTLFSDKNDIYSHQVRIVLAEKGVPYEIENVMLG
                     SISEDLLELNPYGNIPTLVDRELVLFNPRIIMEYLDERFPHPPLMPVYPVSRGQCRLN
                     MYRIEQDWYSQIDIVNKDPESAEGKKALAQLKEDVLALAPIFAAKPYFMSDDFSLVDC
                     YIAPLLWRMQILGVKFSGATAKPINAYMTRVFQRDSFIQSVGGEAPKHLMDDKD"
     gene            3734..4231
                     /gene="purE"
                     /locus_tag="PXA33_001027"
     CDS             3734..4231
                     /gene="purE"
                     /locus_tag="PXA33_001027"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HDL5120369.1"
                     /translation="MPNKAEIAIVMGSKSDWATMSEATQILDQFGLSYHVEVVSAHRT
                     PDKLFSFAETAETNGYKVIIAGAGGAAHLPGMIAAKTIVPVLGVPVKSSMLSGVDSLY
                     SIVQMPKGIPVGTLAIGPAGAANAGLLAAQILAAFNPDIAEKLRIFRQTQTQMVLDNP
                     DPRVE"
     gene            4246..5211
                     /locus_tag="PXA33_001028"
     CDS             4246..5211
                     /locus_tag="PXA33_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HDL5120370.1"
                     /translation="MSKKLLILHTGGTISMKEGEDGKVSPSNENPLLAALERLNHPAK
                     LAQESLFNIPSPHINIEHWQQLKMRIEKAVNEENVDGVVITHGTDTLEETSYFLDLAL
                     SVNIPVVITGAMRSSNELGADGLINLQSAILVALNEESRDKGVLVVMNDEIHNAKFVT
                     KTHTTNVATFQTPTFGPCGLVTKESVIFFQKLTTYERYPIQQVTKANVQLLKAYAGMD
                     SFLLEQLAKSKCDGVVIEALGAGNLPPSCLAGIEALVNANIPIVIVSRAFNGITQDVY
                     DYVGGGKQLKQKGIIFTKGLSGQKARIKLMVLLSQTLDKPLSDYF"
     gene            5230..6318
                     /gene="purK"
                     /locus_tag="PXA33_001029"
     CDS             5230..6318
                     /gene="purK"
                     /locus_tag="PXA33_001029"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HDL5120371.1"
                     /translation="MQKSAIYPPVYVLGNGQLGRMLRYAGAPLDIEVKPLAFDAPVFD
                     IEPNSIITAEIERWADTPLTQLLGNHPNFVNLNVFGKLADRFTQKSLLDELHLATSKW
                     QLLENAEHWQDIFQHIGEKVVVKRRTGGYDGRGQWIVTQDSIHQITPELFGEVIAEKF
                     IPFEGEISIVGARFRDGSTRFYPISHNLQQNGILRYSVSDVNLPNQALYQQQAEQMLA
                     TVMQKLEYVGVMAMECFVVGDKLLINELAPRVHNSGHWTQLGCAISQFELHLRALLDL
                     PTPALDQIAPSVMVNLIGIEHNKQWLSLPFSQLHWYGKEVRAGRKVGHINLTHTDKNG
                     LIELLEQLRPSLTEDFHSGLDWAINKLK"
     gene            complement(6539..6949)
                     /locus_tag="PXA33_001030"
     CDS             complement(6539..6949)
                     /locus_tag="PXA33_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5120372.1"
                     /translation="MISRSLFSKLILFAKIVIFSQLVFMVVIWIAHHIMREYSNPFER
                     FGTLFNNVFLTFIIPMMVSSAVIVYKGYTQKNEDKVFWIYMGFFLIWILGFIYFEPAV
                     YDKSLKISGAGFGAMLSIIICSKLLPKETNKEQL"
     gene            complement(6952..8970)
                     /gene="uvrB"
                     /locus_tag="PXA33_001031"
     CDS             complement(6952..8970)
                     /gene="uvrB"
                     /locus_tag="PXA33_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HDL5120373.1"
                     /translation="MSKQGKPFILHSPFKPSGDQPSAIAKLTEGLNDGLAHQTLLGVT
                     GSGKTFTIANVIAKLNRPAMVLAPNKTLAAQLYAEMKAFFPENAVEYFVSYYDYYQPE
                     AYVPASDTFIEKDASINEQIEQMRLSATKSFLERRDTIVVASVSAIYGLGDVDAYMQM
                     MLHLQVGAMIGQREILSRLAELQYTRNDQAFQRSTFRVRGDVIDIYPAESDEVALRVE
                     LFDDEIENLSLFDPLTGHSLGKVPRYTIYPKTHYVTPRERILEAIEQIKVELADRRTY
                     FIKENKLLEEQRIAQRTQFDIEMMNELGYCSGIENYSRYLSGRKEGEAPPTLFDYMPA
                     DGILVIDESHVTVPQIGGMYRGDRARKETLVQYGFRLPSALDNRPLRFEEFERLSPQT
                     IYVSATPGAYELEKNPDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIHKRVAVDER
                     VLVTTLTKKMAEDLTDYLDEHGVRVRYLHSDIDTVERVEIIHDLRMGMFDVLVGINLL
                     REGLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNLNGKAILYGDRITNSMQKA
                     ITETERRREKQMKYNEEHGITPQALNKKVGELLDIGQSDKPKRGKQAVKKAENSATDY
                     KPKSRKELEKELKTLEQQMRDFAKDLEFEKAAAVRDKLQHLKAYLLEV"
     gene            complement(9209..9445)
                     /locus_tag="PXA33_001032"
     CDS             complement(9209..9445)
                     /locus_tag="PXA33_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HDL5120374.1"
                     /translation="MSRTTSVTLAEPLSQFVNQMVESGRYGSTNEVISAALKILEAQE
                     QQLIQLRALIDEGLESGVSEETVQSIISKAKRNV"
     gene            9608..10453
                     /locus_tag="PXA33_001033"
     CDS             9608..10453
                     /locus_tag="PXA33_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005823485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EfeM/EfeO family lipoprotein"
                     /protein_id="HDL5120375.1"
                     /translation="MFLLKGNFYAFNQTCFCTFALSGFFVATQAAALDLTKETEAYKQ
                     FVVEQIDQLVTDTEKFVGYLNKGDVQKAKKIYPLARMYFERSEPIAESFGDLDPRIDA
                     RLADLAEEGKTEKDWSGFHKIEKVLWQKNTTKGTKATAEQLLKDVKELRAKIPTAEVT
                     PEPMITGAVDLLNEVSTTKVTGEEEIFSKTDLYDFKANIEGAEKIYEIFKTQLEQIDA
                     KLSTEIGSRFAAVNALLAKHNKSKTGYDYVPYNKLSKADIKALAEAVNQLGEPLAQLG
                     VLLNK"
     gene            10464..11660
                     /gene="efeB"
                     /locus_tag="PXA33_001034"
     CDS             10464..11660
                     /gene="efeB"
                     /locus_tag="PXA33_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HDL5120376.1"
                     /translation="MMAETTSRRDFLKHTALVGAGLLSAPAFALESRQISEKSQNQYD
                     FYGKHQAGIVSPAQKHIYFLVLDLDTTDIAKVKTLFQNWTEYSRNLTLGKNVKPYSAN
                     AYVPPADTGEADSLSAQNLTLTFGVSANFFTKLGIEKHKPKELNDLPHFPRDQLQAEY
                     TGGDICIQACADDPQVAFHAVRNLVRVARGEIKMKWSQMGFNSFANQDTPRNLFGFKD
                     GTANAESLKDQENTVWVQQEGWLKGGSYLAVRRVKMFLETWDRTHLQSQEETFGRHRD
                     SGAGIGHQHEFDKLDLAKKDEKGNAVIPEVSHAHLANKTGLKILRRSFSYSSGIDDKG
                     QFDSGLLFVSFQQSPEQFIKIQNALGNVDKMNEYITHIGSGLFACFAGVKDENDYLGK
                     ALFDLI"
     gene            11671..12290
                     /locus_tag="PXA33_001035"
                     /pseudo
     CDS             11671..12290
                     /locus_tag="PXA33_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596976.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+/Pb2+ permease"
     gene            complement(12326..13144)
                     /locus_tag="PXA33_001036"
     CDS             complement(12326..13144)
                     /locus_tag="PXA33_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HDL5120377.1"
                     /translation="MKYDLHAHSTASDGVLSPTELVQRAVEQGVTMLALTDHDTISGI
                     QEAKNFAQNQPLELISGVEISILWQEKSIHLAALNIDENNEELVKFLDNQAQLREERA
                     VEIGEKLAKVGIANAYEGAKALATGEVTRAHYGRFLYEQGYVRNIEHAFKRYLGMGKP
                     AYVKPRWSSLEDAVNVTHTSGGVISIAHPLRYKMTARWIRRLIADFKQAGGDGIEVSG
                     CGQTPDQRQLLARWAKEFDLYGSVGSDFHYPTGWIELGKSLELPQECKPIWTLF"
     gene            complement(13156..13755)
                     /locus_tag="PXA33_001037"
     CDS             complement(13156..13755)
                     /locus_tag="PXA33_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HDL5120378.1"
                     /translation="MTMDNLPLEQHPFDPILPEQATVVMVGTFPPTSEKRCMEFHYPN
                     FQNDMWRIMGNIFYADPNYFRVGEEKRFDPIRIEAFLREKGVALCSSAKTAIRLKGNA
                     SDKDLKIVDPIDMDELLLKLPKLKWLFTTGGLATDTLLGLLPEKHKAPKTNEWVVYPY
                     SATRELYLYRLPSTSRAYPLSLANKTEAYRQFFRTAGLI"
     gene            complement(13752..15299)
                     /gene="sbcB"
                     /locus_tag="PXA33_001038"
     CDS             complement(13752..15299)
                     /gene="sbcB"
                     /locus_tag="PXA33_001038"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HDL5120379.1"
                     /translation="MSDKFSFFFYDYESFGVSPALDRPAQFAGIRTDADFNIIGEPVM
                     FFCKQTPDYLPSPEAVMVTGITPQQCNAEGVSEPEFAARIHAEFSRANTCTIGYNNIR
                     YDDEMTRYTFFRNFYDPYEYSWKNGNSRWDLLDLVRACYALRPDGIEWPTDETGMPSF
                     KLENLTKANGIAHENAHDAMSDVYATIAMAKLIKQKQPKLFDFFFNLRSKKEVEKLID
                     TGEMTPLVHVSGMLGNYRGNTAWVVPLAWHSTNQNAVIVCDLSGDMNGLLTENAEVLR
                     QRLYTKRDELAENELPVPLKLVHINKCPILAPAKTLLPENAERLGIDRALCLENLKTL
                     KAQKSLIREKVIEIFNEERTFEPSTNVETTLYDGFFSPADKNNMAILRTLPPEDLANH
                     GLKFEDPRVEALLFHYRARHYPQTLSRAEQIKWQKHCNQQIEAKAAQFAQSIDDLFQQ
                     HHDNPEKVKLLENLTAYAEQISQQQAVIYRQNVAKDEKLLSELNRVAEQPLDKTEKLK
                     MLKELIK"
BASE COUNT         4618 a         3351 c         3066 g         4437 t
ORIGIN      
        1 agttgatttt tttagacact atttttacga tagcaagcct taacacaaat tacaatataa
       61 agaagagaga aaatggataa actaaacgca atcagtttat tttgtaaagt aattgaaacc
      121 caaagtttca ctcaagcagc acaacaagct caaatttcgt tacccatggc aagtaaattg
      181 gtggctcagt tagaagaaca tctcaatgtg cgtttactac aacgaaccac tcgaaaaatt
      241 acgccaactg aggcagggtt gctctactat cagcaatgct tgcctatttt aaatgaacta
      301 aaagaggccg atgccagcgt aagtaatatt aactcaacgc tacaagggaa aataacaatg
      361 tctgtaccaa tggattttgg ctctcgcttc attgcccctt accttgggaa atttatggaa
      421 gattacccta atttagccct tcatattgaa tttagtgacc gtcgggtaga tgtgattgca
      481 gaaggctttg atttggtgct gcgtattggt agccttgcag atagttccat tgtagcgaaa
      541 aaaattgcag attcccattt ggttttagtt gcttctccag attatttagc tcgatttggc
      601 gaacctaaag gcttagaagc attagtggaa cgagactgcc tactctatga ataccatcct
      661 caatggcaat tttctcaaca aggacaaaaa atgcaaatta aaccacaagg taaaatttac
      721 agtaacaatg gttatgcgtt agtacaaatg gcaaaagctg gtttgggcat cattaacatt
      781 ccaaagtttt taataaaaca cgagctaaaa aacggagagt tgattcctat tatgcctttt
      841 ttgacacagg aacctttaca aatcagctta ctctatccaa accgtcgtta tctctctcct
      901 aaagtgcgaa tcctaattga ttttttcaca caactgatgc aagagcaaaa agagtatatt
      961 taacaaaaaa gaagcagtta taaaacaact gcttcctttc tgaaatacga aattagttaa
     1021 ttatgcttaa ttttctcaaa atgaaaatca aaagagctgc ccctaataca ccaaaaaaga
     1081 taccgactat attaaagtct ccggcactat aagcggaacc aatacctaat acatcaccaa
     1141 atacccagcg tccaagtgac gaacccaaaa taccaataat aatatttttg caaagtccgc
     1201 cttcactttt cattacagcc gaagctatcc aaccaattat agcgccaaca ataattgcag
     1261 caatccatcc cataatattc tccttagctg agttgaaatg aatttttact ctacaagatt
     1321 atgacaaata aaacaaattt ttatttttta cactaaaaag tttgtttagc ttctttgatt
     1381 aatacctctg gtatagtaat gcccaattct tttgccgttt tagtattaat tgaaagctct
     1441 aatttgctga cttcttggat tggaattgta cctgcttttt ccccttttaa tacacgagca
     1501 gcaactttac cggttgccac accaatatca tattgattaa ccgcataagc tgccactgca
     1561 ccacgttgta ctgtatcacg gtcagcagca atcaccggaa tttttgcttc taaagccgct
     1621 ttatgtaagg cttcataagc cgaaacaact gcattatctt cagaaatata aatagcctgc
     1681 acttttccgt ttaggctgcg agcagcagtg ccaatatcgg cactgcgttg aacagcaacc
     1741 ggcactatag taaagccctg tttttgagcc tcttgtttta actcttctaa cacaatcgca
     1801 gaattaactt cacctgcact atacacataa ccaacagctt tcaaatccgg tactaatgtt
     1861 tgaattaatt tcacttgtgg agccatcgct ttgtggtctg aaatccccgt cacgttagtg
     1921 ccacttggct cccacgattt tactaatttt gcagcaaccg ggtctgtcac gcctgaaaac
     1981 acaattggga tagaacgtgt tgcagcaaca acaggttgtg ccgaaggtgt ggtgatcggg
     2041 ataataatat ctgctttatc cccaacaaat ttacgcgcaa tttgtgctgc tgttgcagta
     2101 ctaccctgtg cagactgata gtcgatttta atgtttttac catcaacaaa cccttctcgg
     2161 gctaattctt caatcacacc tttacgaatt gaatctaatg cagggtgttc cacaatcgct
     2221 gtaattgcta cattttgaat tttatcttga gctaatgccg ctacagaaag tgaacttaac
     2281 aaagtcacta ataaggtttt atggaatttc attttctctc cttaagatca ttactgtact
     2341 atcacaacag tacaaacaca aaacaaagat aagcaatttt tagtcggagt gcaagtggaa
     2401 tttgattatt tccgtcacaa aacaaacaag cggttaaatt ttgactgata tttgcaaata
     2461 atcagaaaaa tttaaccgct tgtaatgtgt tttgatgaaa tagatctctt atttcaccaa
     2521 ctttaaggta gatgttttct tcgccttgat ttttttaggc tgctctactg ctctaactcg
     2581 agcttgataa gcctcttcag tgtagtaggc ttcttcttgg aacataatac catcaccggt
     2641 ttcttgcgca taaatcgcca ccattgcccc aaaaggaata taaatatctc ttaacattcc
     2701 ctgaaaacga gcgctaaagc taatgtattc atcagccaca atatactgcc cgatggaacg
     2761 aggcgaaata tttaaaataa tcttcccttc tttgacaaac tctaccggca catctacgtc
     2821 tggaaattct gcattcacta ataagtaagg cgtgttatcg ctatcaataa tccagttata
     2881 gtaggcatta taaagataag gtcttaatgg tttcattagt ctttatcgtc cattaaatgt
     2941 tttggcgctt cgccgccaac agactggata aaactatcac gttggaatac acgggtcata
     3001 tacgcattaa ttggttttgc cgttgcaccg ctgaatttaa cgcctaagat ttgcatacgc
     3061 cataataacg gtgcaatata gcaatcaacc aagctgaaat catcgctcat aaaataaggt
     3121 ttagcggcaa aaataggcgc taatgccaat acatcttctt taagctgtgc taacgctttc
     3181 ttgccttccg ctgattcagg atctttattc acaatatcaa tttgcgaata ccaatcttgt
     3241 tcaatacgat acatatttaa acggcactga ccacgagaaa cagggtaaac cggcattaat
     3301 ggtgggtgag ggaaacgctc atctaaatat tccataataa tacgagggtt aaacagcact
     3361 aattcacgat caaccaatgt aggaatatta ccgtaaggat tcaattctaa taaatcttca
     3421 gaaatactgc ctagcataac gttctctatt tcataaggca cccctttttc tgctaacaca
     3481 atacgcactt ggtggctgta aatatcattt ttatccgaaa aaagagtcat taccgaacgt
     3541 ttgtttatac tactcgtcat ttatatctcc gatgattaaa tttataaaaa ataaaatggt
     3601 tgtcgattat atcatatttt aaccagcaga taacagccct aaaaattgct tttcacttct
     3661 taaatagcta cgcaaacgtt tgctttgtat gctatgatta cactgtttta cattagtatc
     3721 ataaggattt aaaatgccaa ataaagctga aattgcgatc gtaatgggat ctaagagtga
     3781 ttgggctaca atgtctgaag caacccaaat attagaccag ttcggtttga gctatcacgt
     3841 tgaagtcgtc tcagcacacc gcacaccgga caaactcttt tcttttgctg aaactgcaga
     3901 gactaacggc tataaagtga ttattgccgg agccggcggt gcagctcact tgcccggaat
     3961 gatcgctgct aaaaccattg tgccggtgtt aggtgtgccg gtaaaaagct caatgttaag
     4021 tggtgtagat agcctctatt cgattgtgca aatgccgaaa ggtattccgg tcggtacgct
     4081 cgcaatcggg ccggcaggtg cagcaaatgc aggcttatta gccgctcaaa ttttagcagc
     4141 ctttaaccct gatattgcag agaaactgcg tattttccgt caaacccaaa ctcagatggt
     4201 attagataat cctgatcctc gtgttgaata actattgtat aaaagatgtc taaaaaatta
     4261 ttaatcctcc acactggtgg tactatttca atgaaagaag gggaagacgg taaggtctcc
     4321 ccttccaatg aaaatcctct cttggctgcc ttagaacgtt taaaccaccc agcaaaattg
     4381 gcgcaagaat cgttatttaa tattccctct ccgcatatca atatcgagca ttggcaacag
     4441 ttaaaaatgc ggattgaaaa ggcagtgaat gaggagaatg tcgatggcgt agtaatcacc
     4501 cacggcacag ataccttgga agaaacctcc tattttctgg atttagccct cagcgtaaat
     4561 ataccggtag taattaccgg agcaatgcgt tccagtaacg aactgggtgc tgatggctta
     4621 atcaatctac aaagtgcgat tttagtggcg ttaaatgaag aaagccgaga taaaggcgtg
     4681 ttagtggtga tgaatgatga aatccacaat gctaaatttg tgaccaaaac ccacaccact
     4741 aatgtggcga ctttccaaac cccaaccttt gggccttgtg gcttagtaac gaaagaatct
     4801 gtcatttttt tccaaaaatt gaccacttac gaacgttatc caattcaaca agtaactaaa
     4861 gctaacgtgc aactactgaa agcatacgcc ggaatggata gttttctgct ggaacaatta
     4921 gcgaaaagca aatgcgatgg tgttgtgatt gaggcattgg gtgcaggtaa tttaccccct
     4981 agttgtctgg ctggtattga ggctttagtg aatgccaata ttccaattgt aattgtttct
     5041 cgggcattca atggtattac ccaagatgtc tatgattatg taggcggtgg aaaacagtta
     5101 aagcaaaaag gcattatttt taccaaaggc ttaagtgggc aaaaagctcg cattaaactt
     5161 atggtcttac tcagccaaac cttagacaaa ccactatcag actatttcta acccattgag
     5221 aataccacga tgcaaaaaag tgcaatctac ccacctgtgt atgtattagg caacggccag
     5281 ctcgggagaa tgctacgcta cgccggagca ccacttgata ttgaagtcaa accgctggct
     5341 ttcgatgccc ctgtgtttga tattgagcca aacagcatta ttaccgcaga aatcgaacgt
     5401 tgggcagata cgcctttaac gcaattactc ggtaaccacc cgaattttgt gaacctgaat
     5461 gtttttggga agttagccga tcgttttacg caaaaatcac tacttgatga actccatctt
     5521 gcgacttcaa aatggcaact gctagaaaat gccgaacatt ggcaagatat ttttcagcat
     5581 attggcgaaa aagtagtggt taaacgccga accggaggat atgacggacg tgggcaatgg
     5641 attgtaacgc aagactcgat tcatcaaatt acacctgaat tatttggcga agtgattgcc
     5701 gaaaaattta tcccgtttga gggggaaatt tccattgttg gggcgagatt ccgtgacggc
     5761 tcaacccgtt tctaccctat ttctcacaat ttacaacaaa acggcattct acgttactcc
     5821 gtctccgatg tgaatttacc gaaccaagcc ctctaccagc agcaagccga acagatgctc
     5881 gctacggtaa tgcaaaaatt agagtatgtc ggcgtaatgg caatggaatg ctttgttgtg
     5941 ggcgacaagc tactcatcaa cgagcttgcc cctcgagtgc ataacagcgg acactggaca
     6001 caactcggct gtgccattag ccaatttgag ttacatttac gggcattatt agatctacct
     6061 acgccagcac tcgaccaaat tgccccaagc gtgatggtta atttaatagg cattgaacat
     6121 aacaagcagt ggctaagcct cccatttagc caactacatt ggtatggcaa agaagtccga
     6181 gctggtagaa aagttgggca tatcaacctc actcacaccg ataaaaacgg cttaattgaa
     6241 ctactggaac aactcagacc gagcctaacg gaagatttcc attcgggatt agattgggca
     6301 atcaataagc tcaagtaagc ggtcggattt tgtaactttt ttgcaaatct gtttacccga
     6361 tggtgcgagc ttcctcgctc gtaccaaaca agcggtcgac ttagtgctaa aatttgcaaa
     6421 ataattcaac ataatggcta cttccggtca tcatcttgat ctgaaaaccc ctcagggtgg
     6481 atatggctat caaaaagcat tttcttcctc cgagaaaaca gatttcttca caagaatctt
     6541 agagctgctc tttatttgtt tccttaggca ataacttcga gcaaatgata atagagagca
     6601 tagccccaaa tcctgcacct gaaatcttca gtgatttatc ataaactgcc ggctcaaaat
     6661 aaataaatcc caaaatccaa attaaaaaga accccatata aatccaaaac actttatctt
     6721 catttttctg ggtataacct ttataaacaa taaccgcact acttaccatc atcggaataa
     6781 taaaagtcaa aaacacatta ttgaacaggg taccaaatcg ttcaaacgga ttactgtact
     6841 cccgcataat atggtgggca atccagataa ccaccataaa aacaagttga gagaaaataa
     6901 caattttggc aaataagatt aattttgaaa ataatgaacg agaaatcata tttaaacctc
     6961 caacaaatac gcctttaaat gctgcaactt atcccttacc gcagccgctt tttcaaattc
     7021 cagatctttc gcaaaatcac gcatttgttg ttccagcgtt ttaagctctt tttccagttc
     7081 tttacgggat ttcggcttgt agtcggttgc agaattttcg gcttttttga ccgcttgttt
     7141 gccccgtttt ggcttgtcgg attgtccgat gtcgagcaat tcacccactt ttttgttcaa
     7201 cgcttgtggc gtgatgccgt gttcttcgtt gtatttcatc tgcttttcac ggcggcgttc
     7261 ggtctcagta attgcttttt gcattgagtt ggtaatgcgg tcgccgtata aaatcgcttt
     7321 tccgttgagg tttcgggcgg cacgtccgat ggtctgaatt aacgagcgtt cggaacgtaa
     7381 aaatccctct ttatccgcat caagaatggc aaccagcgac acttcgggca tatctaagcc
     7441 ctcacgcaat aagttgatcc ccaccagcac atcaaacatt cccattcgta aatcgtggat
     7501 aatttcgacc cgttccaccg tgtcaatgtc gctatgtaga taacgaactc gtacgccatg
     7561 ttcgtccaaa taatcggtga gatcttccgc catttttttg gtgagtgttg tcaccagcac
     7621 ccgttcgtcc accgccactc gtttgtgaat ttcagacagt aaatcgtcca cctgcgtagc
     7681 aacggggcga acttcgataa tcggatcgag caagccggtt ggacgaacta cttgatccac
     7741 cacatcggga tttttctcca attcatacgc ccccggtgtg gcagaaacgt aaatcgtctg
     7801 tggtgaaaga cgttcaaact cttcaaatct caacgggcgg ttatccagtg cagacggcag
     7861 acggaaaccg tattgcacca atgtttcttt tctcgctcgg tcgcctcgat acattccgcc
     7921 aatttgtggc acagtaacgt ggctttcatc aatcaccaaa ataccgtccg ccggcatata
     7981 atcaaacagg gtcggagggg cttcgccctc ttttctgccc gagagatagc gtgagtagtt
     8041 ttcaatgccc gaacagtagc ccagctcgtt catcatctca atatcaaact gcgtacgttg
     8101 ggcgatccgc tgctcttcca gcaatttatt ctctttgatg aaataggtgc gacggtcggc
     8161 aagctcgacc ttaatttgct cgattgcctc caaaatccgt tcccgagggg tcacatagtg
     8221 agttttcggg taaatggtat agcgaggcac cttgcccaag ctatgccctg taagcggatc
     8281 aaacagcgac agattttcga tttcatcgtc aaacagctct acccttaacg ctacttcatc
     8341 gctttcggca gggtaaatat caatcacatc gccccgcact cggaaagtcg aacgttggaa
     8401 cgcctgatcg ttgcgagtat attgcaattc ggcaaggcga gagagaattt cacgctgccc
     8461 aatcatcgcc cccacttgca aatgcaacat catctgcata taagcgtcca catcacccaa
     8521 gccgtaaatg gcagaaacgg acgccactac aatcgtatct cggcgttcca aaaaggattt
     8581 ggttgcggaa agtcgcattt gctcgatctg ctcattgata gaggcatctt tctcgataaa
     8641 ggtatcgctt gccggcacat aggcttcggg ctggtagtaa tcatagtaag agacgaaata
     8701 ctccaccgca ttttccggaa agaacgcttt catctcggca taaagctggg cggcaagggt
     8761 tttattcggg gcaagcacca tcgccgggcg attgagcttg gcaatcacat tagcaatggt
     8821 aaaggttttc cccgagccgg ttacgccaag taaggtttga tgtgccaagc cgtcatttaa
     8881 gccttcggtc aatttggcaa tcgcacttgg ttggtcgccg gagggcttga aaggagaatg
     8941 gaggataaag ggtttgcctt gcttgctcat agaaaatcct gtcttaaaaa atcggtttat
     9001 tttagcacaa gcaagcggtc gtttttatcg ggatttttgc aaatttattg taactgctta
     9061 tattgatcca ttcttggatc taacacccta acaataaaga taccttttgg gattggttta
     9121 taataaatag aatgtgagcg gtggtaatga cagcgtaaca aagggcgaat aaagtaacgc
     9181 taaatctcgc tctgccctaa cagaaagttt atacattacg ttttgccttg ctgataatag
     9241 attgaacggt ttcctcagaa acaccgcttt ctaagccttc atcaattaag gctcttagct
     9301 gaattagttg ctgttcttgt gcttccaata tttttaatgc ggcactgatg acctcgttgg
     9361 tcgaaccata acgcccactt tcaaccattt ggttcacaaa ttggcttaat ggctcagcta
     9421 aagttacgct ggttgttcta ctcattacta ttcctatctt attgattatt gagcagattg
     9481 taatggctct aatttagata agcaagcggt ctatttttag caataatttg caaaacaaaa
     9541 tgtgatttac atcacaaaat cagcattatt ttttgcgaat aattcgcaat aaggatatta
     9601 tgtcgcaata tttttattaa aaggaaattt ctatgcgttt aaccaaactt gcttttgcac
     9661 ttttgcactt tctggctttt ttgtggctac tcaagcggca gctttagact taactaagga
     9721 aaccgaggct tataaacaat ttgtggtaga acagattgac caactggtta ccgatacgga
     9781 aaaatttgtc ggctacttaa acaaaggcga tgtgcaaaaa gcgaagaaaa tttacccgct
     9841 tgcaagaatg tattttgaac gttctgaacc gattgcagaa agttttggcg atttagaccc
     9901 tcgtattgat gcccgtctgg cagatttagc cgaagaaggt aaaaccgaga aagattggtc
     9961 aggtttccat aaaatcgaga aagtattgtg gcagaaaaac accactaaag gcacaaaagc
    10021 aacggcagaa caattattaa aagatgtaaa agagctgcgt gcgaaaattc ccactgcaga
    10081 agttacccct gagccgatga ttaccggtgc ggtagattta ttaaacgaag tttctactac
    10141 taaagtaacc ggtgaagaag aaattttctc taaaaccgac ctttacgatt ttaaagccaa
    10201 tattgaaggt gcagaaaaaa tctatgagat ttttaaaacg caattagagc aaattgatgc
    10261 caaactctca acagaaatcg gctctcgttt tgcggctgtc aatgcgctgt tagcaaaaca
    10321 caataaatct aaaaccggtt atgattatgt gccttataac aaactgtcaa aagcggatat
    10381 taaagcgtta gcagaggctg taaaccaatt aggcgagccg ttagctcaac ttggcgtact
    10441 tttaaataaa tagtggagca aaaatgatgg cagaaaccac ttctcgccgt gattttttaa
    10501 aacataccgc attagtagga gcaggcttat tgtctgctcc tgctttcgct ttggaatcac
    10561 gacaaatatc tgaaaaatct caaaatcaat acgactttta cggcaaacat caagctggta
    10621 ttgttagccc tgcacaaaaa catatctact ttttagtgct ggatttagac accactgaca
    10681 ttgccaaagt aaaaacattg tttcaaaact ggacggaata tagccgtaac ctcacgcttg
    10741 gcaaaaatgt gaaaccttat agtgctaatg cttatgttcc accggcggat accggtgagg
    10801 cagatagcct ttcggcacag aatctcactc ttacttttgg cgtcagtgcc aatttcttta
    10861 caaagctcgg cattgaaaaa cataagccga aagaactgaa tgatttaccg catttccctc
    10921 gtgatcaact acaagcagaa tacaccggtg gcgatatttg cattcaagcc tgtgcagacg
    10981 atccgcaagt ggctttccac gctgtgcgta atttggtgcg tgttgcccgt ggtgaaatca
    11041 aaatgaaatg gtcgcaaatg gggtttaact cctttgccaa tcaagacaca cctcgtaact
    11101 tattcggttt taaagacggc actgccaacg cagaaagttt aaaagatcag gaaaatacgg
    11161 tatgggtgca acaagagggc tggctcaaag gcggttctta tttagccgtt cgccgtgtta
    11221 aaatgttctt ggaaacttgg gatcgcaccc atctccaatc ccaagaagaa accttcggtc
    11281 gtcaccgtga ttccggagcc ggaatcgggc atcagcacga atttgataaa ttagatttag
    11341 ccaaaaaaga cgaaaaaggc aatgcagtaa ttccggaagt ttcacacgct catcttgcca
    11401 ataaaacggg gctgaaaatc ctacgccgtt cattctccta ttcaagcggt attgatgata
    11461 aagggcaatt tgattcaggg ttgttgtttg tctctttcca acaaagtccg gagcaattta
    11521 tcaaaatcca gaacgctctc ggcaatgtgg ataaaatgaa tgaatacatc actcatatcg
    11581 gcagtggttt atttgcctgt tttgccggtg tgaaagatga aaatgactac cttggcaaag
    11641 cgttgtttga tttaatttag ggaaaaggaa atgaaattaa aagcattagt gctaattccg
    11701 ttactctttt cgagtacagg attgcttgcc aaagtgaatg tcagcccctt atttgttcag
    11761 ctttccgatg caatggcaga gagtaaaaaa ggggaatttg caaaatccag ccaaaatttg
    11821 accgctttac agcacgattt tgagcagatt agccagcacg attccctcgc cggagttgaa
    11881 gtacagaccg ctctgaaaaa agcgattgaa acacccaatg ctgaaaactt agagagcctt
    11941 tctaaaaact ctatgcgttt gaaaaagaac aaaaccctag cggttatgct gaaaaacatc
    12001 aggattttgt ggcaaaaatg acgccgcttt atgcagaact tgctcaaacc gtgccaactc
    12061 aagaaatcgc caaaatcaaa tgggcggctt atcagtttgg taagaattgg gtaaaacagg
    12121 aaaaagcagt acgggaaatc agtcttcccc attatggcaa atttgagcgt attcttggtt
    12181 taatgcgaat taatctgaac gctgaaaagc cggatatggc aaaaatcagt gaactggttt
    12241 ccgaactagg tgtggtaatg gcagacttta agcaagttaa agtgaaatag taaaaaatcg
    12301 gggcaaatta tttgccccga taacactaaa ataacgtcca aatcggttta cattcttggg
    12361 gtaattccaa acttttaccg agctcaatcc agccagttgg gtagtggaaa tcagaaccga
    12421 ccgagccata taaatcaaac tcttttgccc aacgggcaag taattgccgt tgatcaggtg
    12481 tttgtccgca gcctgatact tcaatgccat cgcctccggc ttgtttgaaa tctgcgatta
    12541 aacggcgaat ccaacgagca gtcattttat agcgtagcgg gtgagcaatc gaaatcacac
    12601 cgcctgaggt gtgggtaaca ttcaccgcgt cttctaaact actccatctc ggtttcacat
    12661 aggcaggttt ccccattccc agataacgct taaaggcgtg ttcaatattg cggacatagc
    12721 cttgttcata caaaaaacgc ccataatgtg cccgagttac ttcccctgtt gccaacgctt
    12781 ttgccccctc ataggcattt gcaatcccga cttttgctaa tttttcacca atttcgaccg
    12841 ctctctcttc ccttaattgg gcttggttat ccaaaaactt caccagctcc tcgttgtttt
    12901 catcaatatt caacgccgca agatgaatac ttttttcttg ccataaaatc gaaatttcca
    12961 ccccagagat aagctcaagc ggttgatttt gggcaaaatt tttcgcttct tgaatgcccg
    13021 aaatcgtatc gtgatcagtt aaagctagca ttgttacccc ttgctctacc gcacgttgta
    13081 ccaattcagt gggagaaagt acgccatcag aggcggtact gtgggcgtgt aaatcatatt
    13141 tcattttatt tatccttaaa ttaagccggc agtcctgaaa aattggcgat aggcttcggt
    13201 tttgttggct aagctgagtg gataagccct cgacgtcgaa ggcaggcgat ataaataaag
    13261 ttctcttgtg gcggaataag gatagaccac ccattcgttg gttttcggag ctttgtgttt
    13321 ttccggcaat aaaccgagca acgtatcggt tgccaaacca cctgtggtaa atagccattt
    13381 cagtttgggt aatttgagca acagttcgtc catatcgata ggatcaacaa tttttaaatc
    13441 tttatctgaa gcgtttcctt ttaaacgaat cgctgttttt gccgaagaac agagcgcaac
    13501 gcccttttct cgcaaaaacg cctcaatccg aatcggatca aagcgttttt cttcaccaac
    13561 tcgaaaatag ttagggtcag cataaaaaat attgcccata attcgccaca tatcattttg
    13621 aaaattcggg taatgaaatt ccatacaacg cttttccgat gtaggtggaa aagtgccgac
    13681 cattaccacc gttgcttgtt caggtaaaat agggtcaaac gggtgctgtt ctagcggtaa
    13741 attatccatt gtcatttaat caactctttt aacatcttga gcttttcggt tttatctagg
    13801 ggctgttccg ctactctgtt aagctcgctc agcaattttt catcttttgc aacattttga
    13861 cgataaatga ccgcttgttg ttgcgaaatc tgctcggcgt aagcggtcaa attttctaat
    13921 aattttacct tttccgggtt gtcgtgatgt tgttggaaca agtcgtcaat cgattgggcg
    13981 aactgcgctg cttttgcctc aatttgctgg ttgcaatgtt tctgccattt gatctgctcg
    14041 gctcgactga gtgtttgtgg atagtgcctt gctcggtaat ggaatagtaa cgcctctact
    14101 cgagggtctt caaatttcaa gccgtggttg gctaaatctt caggcggtag cgtgcgtaaa
    14161 atcgccatat tatttttatc cgcaggcgag aaaaagccgt catacaaagt ggtttctaca
    14221 ttggtcgaag gctcaaacgt gcgttcttca ttaaaaattt caatcacttt ttcccgaatc
    14281 agcgatttct gtgcttttaa agttttcaaa ttttccaaac ataaagctct gtctatgcct
    14341 aaacgttctg cattttcagg cagtaaggtt tttgccggtg ctaaaatcgg gcatttatta
    14401 atatgcacta attttaacgg cacaggtaac tcattttcgg ctaattcatc tcgtttggtg
    14461 tataaacgct gacgtaaaac ctctgcattt tcagtcaata agccattcat atcgcccgat
    14521 aaatcacaca caattacggc attttgatta gttgagtgcc aagccagtgg cacaacccaa
    14581 gcggtatttc ctcgatagtt gcccagcata ccggaaacgt gcacaagcgg tgtcatttca
    14641 ccagtgtcaa ttaacttctc tacttctttt ttactgcgta aattaaagaa aaaatcgaac
    14701 aactttggct gtttttgctt aattaatttt gccattgcga tggtggcata cacatcagac
    14761 atcgcatcgt gtgcattttc atgggcaatg ccattggctt tggtgaggtt ttccaattta
    14821 aaacttggca taccggtttc atctgttggc cattcaatac catcaggtct taacgcataa
    14881 caggcacgga ctaaatctaa caagtcccaa cgagaatttc cgtttttcca gctatattcg
    14941 taaggatcgt aaaaattgcg gaagaaggta taacgggtca tctcatcatc ataacgaata
    15001 ttgttataac cgatagtaca agtattcgct cggctaaatt cagcgtgaat tcgggctgca
    15061 aattccggct cagatacacc ttcagcgtta cattgttgcg gtgtaatccc ggtaaccatc
    15121 accgcttcgg ggctgggtaa ataatccggc gtttgcttac aaaaaaacat taccggttca
    15181 ccgataatat taaaatccgc atcggttcga ataccggcaa attgtgccgg acgatctaac
    15241 gccggactca cgccgaaact ttcataatcg taaaagaaaa aagagaattt atcgctcatt
    15301 cgttcaattt gttagaaaaa attgggctga ttgtagcaca gtaaggcaga aaggagggaa
    15361 tacaagcggt caatttccgt tagaaatttg caaatgctaa aataaaaaaa gcccctaatc
    15421 aaaaaagcga aaaggggcgg aggtcttacc taatgataat attattttta ta
//
LOCUS       DANLRK010000047        14996 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.047, whole genome shotgun sequence.
ACCESSION   DANLRK010000047 DANLRK010000000
VERSION     DANLRK010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 14996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14996
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.047"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            92..1282
                     /locus_tag="PXA33_001039"
     CDS             92..1282
                     /locus_tag="PXA33_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HDL5120380.1"
                     /translation="MSTSQQLIQQTEHFGANNYLPLPIVISKAKGIWVEDPEGNRYLD
                     MLSAYSAVNQGHCHPKIVQALKDQADKVTLTSRAFYNDQLGIWYEKICKLANKEMALP
                     MNTGAEAVETAIKTARRWGYDVKGIAENQAEIIACVGNFHGRTMAAVSLSSDPDYKRG
                     FGPMLGGIKLVPYGDLEALKAAITPNTAAFLVEPIQGESGILIPPEGFLEQAYELCKE
                     NNVLFIADEIQAGLGRTGKLFATEWEGFEPDIYILGKALGGGVFPISCVLANRDILGV
                     FNPGSHGSTFGGNPLACAVSIASIDVLLEEKLAERSRELGEYFLAELKKIQNPIIKEV
                     RGRGLFIGLELHQPARPFCEKLKEKGLLCKETHDTVIRFAPPLVIEKFNLDWALEKIR
                     EVLA"
     gene            1357..2868
                     /gene="nhaC"
                     /locus_tag="PXA33_001040"
     CDS             1357..2868
                     /gene="nhaC"
                     /locus_tag="PXA33_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDL5120381.1"
                     /translation="MLILQTFSKNQPLVKGGFMSNRTEITDQNIRIPFRYAILPVIIM
                     VSIMAVAIIKFEASPHVPLLIGAISAAVIAMAHGYRWETIEKGIYRGIRMALPAIVII
                     IMIGLTIGAWLGGGIVATMIYYGLKLISPSLFLVTISIICAIVTLAIGSSWSTMGTIG
                     VAAMGIGFSLGIPAPMVAGAVISGAYFGDKMSPLSDTTNLAAGITETNLFEHIRHMFI
                     TTVPAFLIALSVYFYFGMEFRAENLDLAKIEDIMQAMQEHFLISPWLLLVPLLVVVLV
                     AKKVPALPALAAGIILGWLCHVFVQGGDVSVAVITLHDGYKIESGHEMIDKLFNRGGI
                     ESMMYTISLTIVAMTFGGIAEQAGMLHSVVEKILTLAKSAGSLISATIVSAFITNVTT
                     SEQYISILLPGRMYIKAYRDKGLSSRNLSRALEDGGTVTSVLVPWNTCGVYAFSMLQV
                     SAFEYAPYAIMNWLIPLIAIGLATLNIKIERLPPEELAKEPEEITLPKRGCCP"
     gene            2985..6584
                     /gene="putA"
                     /locus_tag="PXA33_001041"
     CDS             2985..6584
                     /gene="putA"
                     /locus_tag="PXA33_001041"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HDL5120382.1"
                     /translation="MMSYQLLPNTTIRPELSRHYRFDEQTLVQNLIEQAHCDEQADNI
                     KKLTTQLVGKVRTARQKASGVDALMHEFSLSSQEGVALMCLAEALLRIPDTATADKLI
                     RDKIAKGDWRSHTGNSPSLFVNAAAWGLVITGKLVSTHSESSLSSALSRLIAKGGEPL
                     IRKGVDVAMRMLGKKFVTGETIQQAIKNGEKRFAMGYRYSYDMLGEAALTAEDAKRYY
                     NDYVASIHAVGATSKGLGVYKSSGVSVKLSAIHPRYSLSQHQRVMDELYPRLKELFLL
                     AKKYDIGLNIDAEEADRLELSLDLMDRLLADPDLAGFNGIGFVVQAYQKRCPYVIEHL
                     IETARKHNRQLMIRLVKGAYWDTEIKRAQMDGAEGYPVFSRKVHTDLNYIVCAKKLLA
                     AQDVIYPQFATHNAQTLATIYHLAQGKRFEFQCLHGMGESLYDQVVGSDNLNVQCRIY
                     APVGTYQTLLAYLVRRLLENGANSSFVSQIVDENLSVDDLAQAPVQKAELTNGTMHPK
                     IPLPVDLFDDNRVNSKGYDLTDAVSLQNLENALNELTKQHYRAEPQLANDNTSGQESR
                     KIYNPANQAELVGEVIDATENDMEQAFESAAAFQSEWANTSPAVRAECLEKMADLMEA
                     NMPQLFDLAVREAGKTLNNAIAEVREAVDFCRYYAKEVRNNIEAYKNPRGIVVAISPW
                     NFPLAIFVGEVSSALVAGNVVLAKPAEQTSLMAHYAVSLFYQAGVPKAALQCLPGRGK
                     TVGVALVADHRVNGVIFTGSTETAKNINRNLHKNEKIIPLIAETGGQNAMIVDSSALG
                     EQVVIDVLNSAFDSAGQRCSALRVLYLQRDVADHILTMLKGAMAELKVGLPQHLTTDV
                     GPVIDKVAQQRLLDHIENLKKSAKSYYQVAISSEIAENGIFVPPTLLEIDHISQLKHE
                     VFGPVLHVIRFDADKLDQVIADINSTGFGLTSGLHSRIDETVDLWLKSVHAGNLYVNR
                     NTVGAVVGVQPFGGMGLSGTGPKAGGPLYLQRLVNRQEWTLNGLQGNAQAVDFDLNFA
                     IRKVLSGAEQQTALDLAKSLARRSPLGKTYPMQGITGEKNEMIFVARPQIGISKGNLA
                     SQVQALIAIVTVGSQAVAEKSSELAKHASAFGKKLQVVDDFNSLDHLSTLIVLDLLSS
                     EEKLHHAERKGAILTYIEGLDLALSLLPLVHEKAISINTAAAGGNASLMSEMD"
     gene            6693..7343
                     /locus_tag="PXA33_001042"
     CDS             6693..7343
                     /locus_tag="PXA33_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595-like element ISMha3 family transposase"
                     /protein_id="HDL5120383.1"
                     /translation="MRKSRLSQYKQHKLIELFVAGVIARTAAELVNVNKTTAAYYFHR
                     LRLLIYQHSPHLEMFEGEIEIDESYFGGHRKGKRGRGAAGKTAVFGLLKRDGKVYTVV
                     VPNTQSATLLPIIREKVKPDSLVYTDFYRSYDVLDVSEFNHFRINHSTHFAENQNHIN
                     GIENFWSQAKRHLRKFNGIPKAHFELYLKECEWRFNHSDLKSQIFLLKQLVKGGLG"
     gene            complement(7344..8231)
                     /locus_tag="PXA33_001043"
     CDS             complement(7344..8231)
                     /locus_tag="PXA33_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide transport protein"
                     /protein_id="HDL5120384.1"
                     /translation="MNKLTQLALISLAFTGSAFAAPKSFTYCLESSPAALNPQIATDG
                     ATLDVGQAIYNRLIDFEKGTTNLTPSLAERWEISEGGKIYTFFLRQNVQFQSNKSFTP
                     SRYFNADDVIFSINRQFDKTNPYHKLSGGNYEFFYGMNMQNIIKNVTKVDDYTVKIEL
                     SEPNAPFLANLAMDFMAIFSAEYANKMQIAGTPEKLDLEPIGTGAFQFYGYQKDAAVR
                     FTAFEHYWEGKAKIDRLVFSITPDASVRLAKLEKNECQAMPYPNPSDLSALKANKDIQ
                     LFEKSGLNIGYITFRGCPR"
     misc_feature    complement(8257..8402)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            complement(8579..9589)
                     /gene="mglC"
                     /locus_tag="PXA33_001044"
     CDS             complement(8579..9589)
                     /gene="mglC"
                     /locus_tag="PXA33_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018355241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HDL5120385.1"
                     /translation="MSALQQNRTLDFFKQNAIYFVLLILLIVIIVQDPSFLSLRNFSN
                     ILSQSSVRLIIALGVAGLIVTQGTDLSAGRQVGLAAVISATLLQSMENMNKVFPNLSE
                     IPIPVVVLTVCAIGAVIGLFNGFVVAYLNVTPFIATMGTMIIVYGINSLYYDAVGSSP
                     IAGFSDDFSTFAQGFFRFGDFRLSYITIYAVIATIIMWTLWNKTRFGKNVFAIGGNPE
                     AARVSGVNVPLNLMGIYMLSGIFYAFGGMLEAGRIGSATNNLGFMYEMDAIAACVVGG
                     VSFAGGVGTIIGVVTGVLIFTVINYGLTYIGVNPYWQYIIKGGIIILAVAIDSLKYAK
                     KK"
     gene            complement(9603..11102)
                     /gene="mglA"
                     /locus_tag="PXA33_001045"
     CDS             complement(9603..11102)
                     /gene="mglA"
                     /locus_tag="PXA33_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005709009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HDL5120386.1"
                     /translation="MTGEVLLTMTNVSKSFPGVKALDNANLTVKSHSVHALMGENGAG
                     KSTLLKCLFGIYAKDEGDILFLGKPVNFKTSKEALENGISMVHQELNLVKQVTVMDNL
                     WLGRYPLKGPFVDHDKMYRDTKAIFDELDIDVDPRDKVAKLSVSQMQMIEIAKAFSYN
                     AKIVIMDEPTSSLSEKEVEHLFKIIQKLKDRGCGIIYISHKMDEIFKICDEITILRDG
                     KWVNTVAIKDTSMDQVVSMMVGRELTQRFPEKTNLPKEVILEVEHLTAKNQPSIQDVS
                     FSLRRGEILGIAGLVGAKRTDIVETIFGVREKAGGTIRLNDKEINNRNAFDAINNGFA
                     LVTEERRSTGIYANLSIEFNSLISNMKSYLGKFGLLSNKKMKSDTQWVIDSMNVKTPS
                     HQTHIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDIGAKFEIYQLILELANKDK
                     GIIMISSEMPELLGVTDRILVMSNGKVAGIVETAHTSQEEILQLAAKYL"
     gene            complement(11174..12163)
                     /gene="mglB"
                     /locus_tag="PXA33_001046"
     CDS             complement(11174..12163)
                     /gene="mglB"
                     /locus_tag="PXA33_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015701875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HDL5120387.1"
                     /translation="MKKAVLSAVAMAVALGATISTAQAENRIGVTIYKYDDNFMALMR
                     KEIEKEGKTQKVELLMNDSQNTQSIQNDQVDVLLSKGVKALAINLVDPSAYKTIIQKA
                     KSEDVPVVFFNKDPGAAAIDSYPKAYYVGTEPKESGIIQGELIAKQWKAHPELDLNKD
                     GKIQFALLKGEPGHPDAEARTKYVVESLKAQGIDSEQIFMDAALWDAAQAKDKVDAWL
                     SSGKAKDIEVIISNNDGMALGALEATKAHGRKLPIFGVDALPEALQLIKKGELAGTVL
                     NDGVNQGKAVVQLSANLADGKDAVAGTQWKLENRVVRIPYVGVDKDNLSEFLK"
     gene            complement(12438..13430)
                     /gene="mglB"
                     /locus_tag="PXA33_001047"
     CDS             complement(12438..13430)
                     /gene="mglB"
                     /locus_tag="PXA33_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HDL5120388.1"
                     /translation="MKRTILSAFALSVICGVNLSYAQSESRVGVTIYKYDDHFMTQMR
                     KDMQNEAKGFAGLKWFMNDAQNSQVKQLAQIETLLQHKMRVLAVNIVNANESKTIVEK
                     AKAYNVPVVFFNRTPSKQALESYEKAYFVSADPKEAGRIQGELIAKVWKSNPDFDLNK
                     DGKLQFVLLKGEPSDVSAERSQAVVESLNQQGVQAEEIYSDTARWRRTLARNKMNEWL
                     VENRAAEIEVVISNNDEMAIGALDALNAHEKRLPIFGIDALPETLALMKKGEIAGTVL
                     NDSSAQAKAVVKLVSNLAQGKPALKDTQWESHSSKTIYLPHIGIDKENLGQFLP"
     gene            complement(13515..13928)
                     /locus_tag="PXA33_001048"
     CDS             complement(13515..13928)
                     /locus_tag="PXA33_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PACE efflux transporter"
                     /protein_id="HDL5120389.1"
                     /translation="MTAWERVFHALLFECFAIIFTVILTSWLTSHRMVDLTAVIVMIS
                     VIAMVWNVIFNWGFDKVFTGERVQRGLGIRILHSFLFEGGLLVFTIPLVMYMLDIGWW
                     QAFVMDIGMTLFVLVYSVVFNWVYDHLRLRWVKSG"
     gene            14048..14335
                     /locus_tag="PXA33_001049"
     CDS             14048..14335
                     /locus_tag="PXA33_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HDL5120390.1"
                     /translation="MKNDKPIECEGCNTFDVGSILDNSETVATIQRTYESENEAKAAL
                     EKFTQKARDVESEPCEISSEITPIESGFELVAHFKFSCQAEVVIFQLGTRF"
     gene            complement(14381..>14996)
                     /locus_tag="PXA33_001050"
     CDS             complement(14381..>14996)
                     /locus_tag="PXA33_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDL5120391.1"
                     /translation="SKYKSLEHINETGFYRYLIPYLDEERVLYLDSDIVVDDNLSEMY
                     WLNFNGIPLYAVEDYVHNHIPAYLDLDLSPYFNSGVLLINNILWKKYSVCDELVTINE
                     KIVNLTYGDQDALNIYFKGGWGALPIEYNYQVDAILELVLRREQEELARKNGYLDVIP
                     KIIHYTSKFKPWKKELHTMQISTRKKYWFYYHLEWNDILEQHQK"
BASE COUNT         4402 a         3200 c         3047 g         4347 t
ORIGIN      
        1 agatcataaa atcaacaaaa agtccgtgct ttttagctaa agattgttaa actcttttgt
       61 gttacaagtt tttaacataa aggagttttt tatgtctact tctcaacaac tcatccaaca
      121 aaccgaacat ttcggtgcca ataactacct acctctacct attgttatta gtaaagctaa
      181 aggcatttgg gttgaagatc ctgagggtaa tcgctatttg gatatgctta gtgcctactc
      241 agcggttaat caagggcatt gtcatccaaa aattgtacag gcactaaaag atcaagcaga
      301 caaagtaacg ctgacctctc gagcatttta taacgatcaa cttggtattt ggtatgaaaa
      361 aatctgcaaa ctcgctaata aagagatggc attaccgatg aatacgggtg cagaagcggt
      421 agaaacagcg attaaaactg ctcgccgttg gggttatgat gtgaaaggaa ttgcagaaaa
      481 tcaagcagaa atcatcgctt gtgttggcaa ttttcacgga cgaacaatgg ctgcagtatc
      541 tctctcgtct gatccggatt ataaacgtgg tttcgggccg atgttaggtg gcatcaaact
      601 tgttccatac ggtgatttag aagcattaaa agctgcgatt acaccaaaca cagcagcgtt
      661 tttggttgag ccaatccaag gtgaatctgg cattcttatt ccacctgaag gtttcttgga
      721 acaggcttat gaactatgca aagagaataa tgtgctgttt attgctgatg aaattcaggc
      781 aggtcttggg cgtactggca agttatttgc aacagaatgg gaaggctttg agccagatat
      841 ttatattcta ggtaaagcac ttggtggcgg ggtgttccca atctcttgtg tgttagcaaa
      901 tcgtgatatt ttaggcgtat tcaatcctgg ctcgcacggt tcgacatttg gtggtaatcc
      961 attagcctgt gcggtatcta ttgcctcaat tgatgtactg ttggaagaaa aacttgcaga
     1021 gcgttcccgt gagttaggtg aatacttctt ggcagagctg aaaaaaatcc aaaatccaat
     1081 tattaaagaa gttcgtggac gtggactgtt tattggcttg gagttacatc aaccagcacg
     1141 cccattctgc gaaaaactca aagaaaaagg cttactctgc aaagagaccc acgatacagt
     1201 tatccgcttt gctccccctt tggtgattga aaaattcaat ttggattggg cattggagaa
     1261 aattcgggag gtgttggctt aattgtcatt ttctctatat tttatcctgc caaatcgcct
     1321 ttaacccttt ttggcaggaa gcaaaacagt tgtaaaatgc tgattttgca aactttttcc
     1381 aaaaatcaac cgcttgtaaa aggtggcttt atgtcaaaca gaacagaaat aacagaccaa
     1441 aatatccgaa tcccgtttcg ctatgctata ttgcctgtga ttattatggt atcgattatg
     1501 gcggtggcta tcatcaaatt tgaagcctca cctcacgttc cgttgcttat tggggcaatt
     1561 agtgccgctg tgattgcaat ggcacatggc taccgttggg agacaattga aaaaggaatt
     1621 tatagaggca ttcgtatggc tttaccagcg attgttatca ttattatgat tgggctaacc
     1681 atcggagctt ggctaggtgg tggaattgta gctacgatga tttactacgg cttgaaattg
     1741 atctctccgt cgctgttttt agtaactatc tcaataattt gtgctattgt gacccttgcg
     1801 attggtagct cgtggtctac aatgggaaca atcggtgtag cggcgatggg gatcgggttt
     1861 agtctcggca ttccggcacc gatggttgcc ggtgcggtga tctcaggagc atatttcggc
     1921 gataaaatgt cgccactgtc agacactacc aatctggcag cagggattac cgaaaccaat
     1981 ctgtttgaac atatccgcca tatgtttatc actaccgtgc cggcgttcct gattgcgtta
     2041 tctgtctatt tctattttgg tatggaattt agagccgaaa atctcgatct tgctaaaatt
     2101 gaagacatta tgcaggcaat gcaggaacat ttcttgattt cgccttggtt gctattagtg
     2161 cctttattag tcgtggtgtt ggtggcaaaa aaagtgcctg ccttaccggc attagctgca
     2221 gggataattc ttggttggct ctgccacgtt tttgtgcaag gcggtgatgt cagcgtagca
     2281 gtgattactc tgcacgacgg ctataaaatt gagagcggcc acgagatgat tgataagctg
     2341 ttcaaccgtg gtgggatcga gtcgatgatg tacaccattt cccttacgat tgttgcgatg
     2401 acttttggtg gcattgcaga gcaagctgga atgttacatt cagtagtgga aaaaatcctt
     2461 acccttgcaa aatcagcagg tagcttaatt agtgctacga ttgtatcggc atttattacc
     2521 aacgtaacga catctgagca atatatttct attttattac caggcagaat gtatataaaa
     2581 gcctataggg ataagggact aagttcgaga aacctttctc gtgcattgga agacggagga
     2641 acagttactt cggttcttgt accttggaat acctgtggtg tttatgcgtt ctcaatgttg
     2701 caagtgagtg cttttgaata tgcaccgtat gcaataatga attggctcat tccactgatt
     2761 gcgattggct tggcaacctt gaatatcaaa attgagcgat tgccaccgga agaattagcc
     2821 aaagaacctg aggaaattac tctgccaaaa cgtggttgtt gtccataatt tatctaaata
     2881 gtatttaata acaataggtt aagttaaata acaagcggtc ggttttatct taaaatttgc
     2941 aagtttatac ataaatttga tcgcttgctg tatagaaagg aaaaatgatg tcataccaac
     3001 ttttacctaa caccactatc cgtcctgaat tgagccgtca ttaccgcttt gatgagcaaa
     3061 ccctggtgca aaatctgata gaacaagcac attgtgatga acaggctgat aacattaaaa
     3121 agctcactac ccaattagtc ggtaaagtcc gaactgcacg ccagaaagca agcggggttg
     3181 atgcgttaat gcacgaattt tcactctcca gccaagaggg ggtggcgttg atgtgccttg
     3241 ccgaagcatt actacgcatt cctgacaccg ccactgcgga taaattaatt cgagacaaaa
     3301 ttgccaaagg ggattggcgt tcacacaccg gcaacagccc ctctctgttt gtgaatgcag
     3361 cggcgtgggg cttggtgatt accggcaaat tggtttcaac ccacagcgaa agctcactct
     3421 catcagcact ttcacgcctg atcgccaaag gcggagaacc gctgatccgc aaaggtgtgg
     3481 acgtagcaat gcgtatgctc ggcaagaaat ttgtaacggg cgaaaccatt caacaggcga
     3541 ttaaaaacgg cgaaaaacgc ttcgcaatgg gttatcgtta cagctacgat atgctgggcg
     3601 aagcggcact caccgccgaa gatgccaaac gctattacaa cgactatgtt gcctctattc
     3661 acgctgtggg tgccacctcg aaaggcttgg gcgtgtataa atcctcgggt gtgtcggtca
     3721 aactctccgc cattcaccct cgttattccc tttcccaaca ccaacgtgtg atggacgaac
     3781 tctatccacg tctgaaagaa ctgttcttgc tggcgaaaaa gtatgacatc ggcttgaaca
     3841 ttgatgccga agaagccgac cgcctcgaat tgtcgcttga tttgatggac agactgcttg
     3901 ccgaccctga tttggcaggg tttaacggca ttggttttgt ggtgcaggcg taccaaaaac
     3961 gttgcccgta tgtgatcgag catttaattg agaccgcccg taaacataat cgccagctaa
     4021 tgattcgctt ggtaaaaggc gcatattggg acactgaaat caagcgggcg caaatggacg
     4081 gtgcagaggg ctatccggtc ttctcccgta aggtgcatac cgatttgaac tacatcgttt
     4141 gtgccaaaaa attgctggcg gcgcaagatg tgatctatcc gcaatttgcc acccacaacg
     4201 ctcaaactct cgccaccatt taccacttgg cacagggcaa acgttttgag tttcaatgct
     4261 tgcacggtat gggagagagc ttgtacgacc aagtggttgg cagcgataat cttaacgtgc
     4321 aatgtcgcat ttatgcccct gtcggtactt atcaaaccct gcttgcctat ttagtacgtc
     4381 gcttgttgga aaacggagcg aacagctcat ttgttagcca aattgtggat gaaaatctca
     4441 gtgtagatga tctggctcaa gcccctgttc aaaaagcaga actgaccaat ggcactatgc
     4501 acccgaaaat tccattgcca gtcgatttat ttgacgacaa tcgtgttaat tccaaaggct
     4561 atgatttaac tgatgcagta agcctacaaa atttagaaaa tgccctcaat gaacttacca
     4621 aacagcatta ccgtgctgaa ccacagctgg caaatgataa tacaagcggt caagaaagcc
     4681 gaaaaattta caatccagcg aaccaagctg aattagtcgg cgaagtgatt gatgccaccg
     4741 aaaacgatat ggaacaggcg tttgaaagtg cggcggcgtt ccaaagtgaa tgggcgaata
     4801 cttcccctgc ggtgcgtgcc gagtgcttag aaaaaatggc ggacttgatg gaagccaata
     4861 tgccacaact gttcgatttg gcggtgcgtg aggcaggcaa aacgctcaac aatgcaattg
     4921 ctgaagtgcg tgaagccgtc gatttctgtc gttattatgc caaagaggta cgcaacaaca
     4981 ttgaagcata caaaaatccg cgtggcattg tagtagcaat tagcccgtgg aactttccgt
     5041 tagcgatttt tgtgggtgaa gtttcatctg cgttggtggc aggcaatgtg gtactagcaa
     5101 aaccagctga acaaacttcg ttaatggcac attatgctgt cagccttttc taccaagcag
     5161 gtgtgccgaa agcggcgttg caatgcttac cgggtagagg aaaaacggtt ggggtggcgt
     5221 tggtggcaga tcatcgtgtg aacggcgtga tttttactgg ttcaacagag acagctaaaa
     5281 acattaaccg caatttgcac aaaaacgaga aaattatacc gcttattgcc gaaactggcg
     5341 gacaaaatgc aatgattgtt gatagctcgg cactgggcga gcaggttgtg atagatgttt
     5401 taaactccgc atttgactca gcagggcaac gttgttcggc attgcgtgtg ctgtatctgc
     5461 aacgtgatgt tgccgatcac attctcacaa tgctcaaagg agcaatggcg gagttgaaag
     5521 ttggtcttcc acaacactta actaccgatg tcggaccagt aatcgataaa gtcgcacaac
     5581 aacgcttgct cgatcatatt gaaaatctca aaaaatcggc gaaaagttac tatcaggtgg
     5641 ctatctcctc tgaaattgcc gaaaatggta ttttcgtacc gccaacgctg ttagaaatag
     5701 atcatatcag ccagcttaaa cacgaagtat tcggaccggt gctacacgtt atccgttttg
     5761 atgcggataa actcgaccaa gtgattgccg acataaactc cacaggtttc ggtctaacca
     5821 gcggtttgca tagccgaatt gatgaaacgg tggatttatg gttgaaatct gtccacgctg
     5881 gaaatctgta tgtaaaccgt aacacggtag gggctgtggt cggcgtacag ccattcggtg
     5941 gaatggggtt atcaggaact gggccgaaag ctggcgggcc gctctatcta caacgtttag
     6001 taaaccgcca agaatggaca ttgaacggtt tacagggcaa tgcacaagcg gtcgattttg
     6061 acttaaattt tgcaatccgc aaggttctaa gtggagctga gcagcaaacg gcgttagatc
     6121 tggccaaaag ccttgcacgc cgctcgccac tcggaaaaac ctacccaatg cagggtatta
     6181 ccggcgagaa aaacgagatg attttcgtgg ctcgcccaca gattgggatt agcaaaggta
     6241 atttggcttc acaggtgcaa gccttgattg ccattgtaac ggtcggcagc caagcggtgg
     6301 cagaaaaatc aagcgagctg gcaaaacacg cctctgcctt tggtaagaaa ttacaggtgg
     6361 tggatgattt caacagtctc gatcacctca gcacactcat tgtgcttgac ctgctttcta
     6421 gtgaggaaaa attgcatcat gccgaacgca aaggggcgat tttgacctac atcgaagggc
     6481 tggatttagc gctctcactg ctgccgttgg tacacgagaa agcaatcagc atcaacaccg
     6541 ctgccgcagg gggaaatgcc agcttaatga gtgaaatgga ttaagcaaca aacaaaagag
     6601 tataagaggg cagatcacaa tgatctgcct ttttatttta aggggctgtc ctagataacg
     6661 actaaaactt ccatttaggt taagataacc ctatgagaaa aagtcgtcta agtcagtaca
     6721 aacaacataa actcatagag ctgtttgtag caggcgtaat cgctcgaaca gccgcagagt
     6781 tagtgaatgt aaataaaacc accgcagcct attattttca ccgtttgcga ttgctcatct
     6841 atcaacatag ccctcatttg gaaatgtttg agggagaaat tgagattgat gagagctatt
     6901 tcggcggtca tcgcaagggt aaacgaggtc gtggtgcagc aggcaaaacc gccgtattcg
     6961 ggcttttaaa gcgtgacggc aaggtttata ccgtagtagt gccgaacaca caatctgcta
     7021 cacttttgcc gattattcgg gaaaaggtga agcccgatag ccttgtttac accgattttt
     7081 atcgaagcta tgatgtgctt gatgtgagtg aatttaacca ttttcgtatc aatcacagca
     7141 cacacttcgc agaaaatcaa aatcacatta acggaattga gaatttttgg agtcaagcaa
     7201 aacgtcattt acgcaagttt aatggtattc cgaaagccca ttttgagcta tatttgaaag
     7261 aatgcgaatg gcgttttaat cacagtgact taaaatctca aatattcctt ttaaaacaat
     7321 tggttaaagg ggggttaggt tagttatcta ggacagcccc taaaagtaat ataaccgata
     7381 tttaggcctg acttttcaaa taattgaata tctttattcg cttttaaagc agacaaatct
     7441 gacgggttcg gataaggcat cgcttggcat tcgttcttct ccaatttagc taaacgtact
     7501 gaggcatcgg gcgtaatact aaatacaagg cgatctatct tcgcttttcc ttcccaatag
     7561 tgttcaaatg ctgtaaatct taccgctgcg tccttttggt aaccatagaa ctggaatgca
     7621 cctgtgccta taggttctaa atctaatttt tccggcgtac ccgctatttg cattttattt
     7681 gcatattccg cagaaaagat cgccataaaa tccatagcta aattagcaag aaagggggca
     7741 ttcggttcag ataattcgat ttttaccgtg taatcatcta cttttgttac atttttgatg
     7801 atgttttgca tattcattcc gtagaaaaac tcatagttac cgccggacaa tttgtgatat
     7861 ggattcgtct tatcaaactg acggttaatc gaaaaaataa catcatcagc attaaaataa
     7921 cgactagggg taaaactttt attactctgg aactgcacat tctgacgcaa gaaaaaagta
     7981 taaatttttc cgccttcact gatttcccat cgctctgcca gagaaggagt gaggttagtg
     8041 gtaccttttt caaaatcaat caggcgatta tagattgctt gcccgacatc aagagtagct
     8101 ccatctgttg caatctgagg attcaatgct gccggagatg attccaaaca gtaagtaaaa
     8161 gatttaggtg ctgcgaaagc agaacctgta aatgctaaag aaataagtgc aagttgtgtc
     8221 agcttgttca ttgtcatatc cttaaatgtt aagcaataaa aaaccctttc aaaagttgcc
     8281 ttccgaaagg gttagaattg ggtttgcgat tttggattgc gtttgccatt tcaggttttc
     8341 ggaagatatc ttccgaacac gccaaatgcc cgaaagatta atcggtcaaa tggtagtgat
     8401 gatgtttaac gcaatagctc ataatattct ccaaaattga atgtttagga atttacgctt
     8461 tttaaaaatc aattgcaaac gttttcttta aaattttgta aaaaattcaa catttttcac
     8521 cgcttgtatc gtacctagga gcaaatggcg atttgctcct aggtttccaa gcaataggct
     8581 attttttctt cgcatattta agtgagtcaa tcgctaccgc caagataatg atgccacctt
     8641 taataatata ttgccagtaa gggtttactc caatataggt taagccgtag ttgataacgg
     8701 tgaagattaa cacccctgtt actacaccga tgatagtacc cacaccaccg gcaaaggata
     8761 ccccacctac cacacaagcg gcaatggcat ccatttcata cataaagcct aagttgttgg
     8821 ttgcactacc gatacgacct gcttctaaca taccgccgaa ggcatagaag atgcctgaaa
     8881 gcatataaat acccatcagg ttaagcggta cgtttacacc ggaaactcgt gctgcttcag
     8941 ggttgccgcc aatagcaaat acgtttttac cgaagcgggt tttattccat aacgtccaca
     9001 taataatggt agcaatcacg gcataaatag tgatgtagga tagcctaaag tcgccaaagc
     9061 ggaagaagcc ttgtgcaaag gttgagaaat catcgctaaa gcctgcaatt ggcgatgagc
     9121 ctactgcatc gtagtaaagc gagttgatac catacacaat aatcattgtt cccatcgtgg
     9181 caataaacgg tgttacgttg aggtaagcca ccacaaagcc gttaaataaa ccaatgacag
     9241 caccgatggc acacacggtt aatacaacca caggaatcgg gatttccgag aggtttggga
     9301 ataccttgtt catattttcc attgattgga gcaaggtggc ggaaattacc gctgccaagc
     9361 ccacttggcg accggcagat aagtctgtcc cttgtgttac aatcaagcct gcaaccccta
     9421 atgcaataat caaacgcaca gaagattgcg ataaaatgtt actgaagtta cgcaagctca
     9481 agaaacttgg gtcttgcaca ataatcacaa taagtaagat taataatacg aagtaaatcg
     9541 cattttgttt aaaaaaatcg agtgttctat tttgttgtaa agcagacata cttcactcct
     9601 tgttataaat attttgctgc gagttgcaag atttcttctt gcgaggtgtg agcggtttcg
     9661 acaatacctg caactttccc gttactcatc actaaaattc ggtctgtgac cccaagcaat
     9721 tccggcattt ccgatgaaat cataatgatg cctttatctt tattcgccag ctctaaaatc
     9781 aactggtaaa tttcaaattt tgccccgata tcgataccac gggtcggctc gtcaagcatc
     9841 aagatttcag gctgggttaa caaccaacgc ccgataatca ctttttgttg gttaccgccg
     9901 gaaagtgagc caatatgcgt ttgatgcgat ggggttttta cattcatcga atcaattacc
     9961 cattgggtat cgcttttcat ttttttattg ctcagtaagc cgaacttgcc tagataagac
    10021 ttcatatttg aaatcagaga gttaaactca atgcttaagt tggcataaat ccctgttgaa
    10081 cggcgttctt ctgtaaccaa tgcaaagccg ttattaatcg catcaaaagc gttgcggtta
    10141 ttgatttctt tatcgtttag tcggatagtg ccgcctgctt tctcccgtac accgaaaatg
    10201 gtttcaacaa tatcagtccg ttttgctcca actaagccgg cgatgcctaa aatttcccct
    10261 ctgcgtaagc taaagctgac atcttgaata gagggttgat tttttgccgt taaatgctca
    10321 acttcaagaa tcacttcttt cggcagattg gttttttcag ggaaacgctg ggtcagctca
    10381 cgccctacca tcattgaaac cacttgatcc attgaggtat ctttaattgc aacggtgttt
    10441 acccatttac cgtcacgcaa aatggtaatt tcatcacaaa ttttaaaaat ttcgtccatt
    10501 ttgtgtgaaa tatagataat gccacagcct ctgtctttca gtttttggat aattttaaat
    10561 aaatgctcga cttctttttc tgaaagtgac gaagttggtt cgtccataat cacaattttg
    10621 gcattgtaag agaaagcttt tgcaatctcg atcatctgca tttgtgaaac cgataatttg
    10681 gctactttat cacgtggatc aacatcaata tctaattcat caaaaatcgc cttagtatca
    10741 cgatacattt tatcgtgatc tacaaaaggc ccttttagtg ggtaacgacc tagccataag
    10801 ttatccatta ccgtcacttg ttttactagg ttcaactctt ggtgaaccat tgagatccca
    10861 ttttctaacg cctcttttga ggtcttaaaa ttcaccggct tgccaagaaa gagaatatct
    10921 ccctcatctt tagcataaat cccaaataag catttcagta acgttgattt acctgctcca
    10981 ttctctccca ttaaggcgtg aacactatgg gatttcaccg ttaagttagc attatctaag
    11041 gcttttaccc cgggaaatga cttgctaaca tttgtcattg tgagtaaaac ttcacctgtc
    11101 atatctcacc cctgattaca tcgaaaaatc gaaaaaaagc acgggtatcc atcacccgtg
    11161 ctaaaagcgt gaattatttt aaaaattcag ataagttgtc tttatcgaca ccaacgtaag
    11221 gaatacgcac cacacggttt tccaatttcc attgtgtacc agcaactgca tctttaccgt
    11281 ctgctaagtt agcagaaagc tgaacaaccg ctttaccttg gtttacacca tcatttaata
    11341 ccgtaccggc taattcacct tttttgatta attgaagtgc ttctggcaat gcatctacac
    11401 cgaagattgg taattttcta ccgtgagctt tggttgcttc taatgcacct aacgccatac
    11461 catcattatt agaaataatt acttcgatgt ctttcgcttt accacttgat aaccaagcat
    11521 ccactttatc tttagcttgg gctgcatccc ataatgctgc gtccataaag atttgctcac
    11581 tgtcgatacc ttgtgctttt aagctttcta ccacatattt agtacgtgct tccgcatcag
    11641 ggtgacccgg ctcacctttt aataaggcaa attggatttt accgtcttta ttcaagtcta
    11701 attcaggatg ggctttccac tgttttgcaa ttaactcacc ttgaataatg cctgactctt
    11761 tcggctcagt acctacataa taagctttcg gatagctatc aatcgcagct gcacccgggt
    11821 ctttattgaa gaacaccaca ggcacatctt cactttttgc tttttgaata attgttttat
    11881 aagctgatgg atccactaag ttaattgcta atgctttaac ccctttagaa agtaatacat
    11941 caacttggtc gttttgaata gattgcgtat tttgcgagtc attcattaat aactcgactt
    12001 tttgtgtttt accctctttt tcgatttctt tacgcattaa tgccataaag ttatcgtcat
    12061 atttataaat tgttacacca atacggtttt cagcttgagc tgtactgatc gttgcaccta
    12121 atgcgactgc cattgccact gcacttaata ctgctttctt catagtagat cacctctttt
    12181 atgtgtttat agggaaatga attgcttacc ataagcaact tggtgcaaag tataactaat
    12241 tttaaaaaaa caatccgtga tcattctcac agaattaaga caaagtggag aaatttttaa
    12301 ttttaagtat aaaatttaaa tattcgctct attcaaatct gttggaagaa aagagaaagg
    12361 atttgcaaga ttttactgaa aaatgaccgc ttgttatcat caataagggg cgtatttata
    12421 acgccccgct cctccattta aggtaaaaat tgccctaaat tttccttatc aataccaata
    12481 tgtggaagat aaatcgtttt agaggaatgg ctttcccact gcgtatcttt cagcgcaggc
    12541 ttaccttgtg ccaaattaga taccaatttc accaccgctt tcgcttgtgc agagctatca
    12601 tttaataccg tgccggctat ttcccctttt ttcattaatg ctaacgtctc aggcaaggca
    12661 tcaataccaa agatcggcaa tcttttctcg tgtgcattta acgcatctaa agcaccaatt
    12721 gccatttcat cattgtttga aatcacaact tcaatctcag ctgcacggtt ttccaccagc
    12781 cactcattca ttttattgcg agccaatgtt ctacgccatc tcgccgtgtc actgtaaatt
    12841 tcttctgcct gaacaccttg ttgattaagc gattcaacca cagcttgaga acgctcagcg
    12901 ctcacatcac tcggctcgcc cttaagcaat acaaattgca atttcccgtc tttatttaga
    12961 tcgaaatcgg ggtttgattt ccacactttc gcaattaatt ccccttggat tctaccggcc
    13021 tctttaggat cagcgctcac aaagtacgcc ttttcgtaac tttctaacgc ttgtttactc
    13081 ggtgtgcggt taaaaaagac aaccggcaca ttataagcct tcgctttttc cacaatagtt
    13141 ttcgactcat ttgcattcac aatattcacc gccaacacac gcattttatg ctgaagtaag
    13201 gtttcaattt gcgctaattg cttcacttgg gaattttgtg catcattcat aaaccacttc
    13261 aacccagcga agcctttcgc ctcgttttgc atatccttac gcatttgagt cataaaatgg
    13321 tcatcatatt tgtaaatcgt tacccccact ctgctttcag attgtgcgta acttagattc
    13381 acaccgcaaa taacagaaag tgcaaaagca ctcaagattg tacgcttcat agaatgtccc
    13441 tcttgctatg ccaaatatga accaattcaa gcatagcgaa ttttgtgtaa ggaaaccgtg
    13501 atctagttca caatttagcc agatttaacc cacctcaggc gtaaatgatc gtacacccaa
    13561 ttaaaaacta ccgaataaac taatacaaat aaggtcattc caatatccat cacaaacgcc
    13621 tgccaccagc cgatatcgag catatacatt accaatggga tagtaaaaac cagcaaaccg
    13681 ccctcaaaaa ggaatgaatg caaaatacga atgcctaaac cacgttgcac tcgctcaccg
    13741 gtaaacacct tatcaaaacc ccagttaaaa ataacattcc acaccattgc aatcaccgaa
    13801 atcatcacaa tcactgcggt taaatcaacc atacgatgag aagtcagcca agaagttaag
    13861 ataaccgtaa aaataatagc gaaacattca aataacagcg cgtgaaacac acgctcccaa
    13921 gcggtcattt ttcctccaaa ctttgcaaaa taccaactaa atcaaataat cgttcactga
    13981 tgattgcagg ttacgcaatt tagttttaaa gtacctacat tattcatcaa tttagaagga
    14041 ttagccaatg aaaaacgata aaccgattga atgcgaaggc tgtaacacat ttgatgtagg
    14101 ttctatttta gataacagcg aaacagtcgc tactattcag cgcacttatg aaagcgaaaa
    14161 tgaagctaaa gcggcacttg aaaaattcac tcaaaaagcc cgtgatgtcg aatctgaacc
    14221 ctgtgaaatc agcagtgaaa tcaccccgat tgaaagcggt tttgaactgg ttgctcactt
    14281 caaatttagc tgccaagcag aagtggttat ttttcaatta ggaacacgtt tctaatacaa
    14341 aaagctgatt caaacacttg aatcagcttt tgtatgatag ctatttttga tgttgttcca
    14401 aaatatcgtt ccactctaaa tgatagtaaa accaatattt tttacgcgta gatatttgca
    14461 ttgtatgcag ttcttttttc caaggtttga atttactcgt atagtggata attttcggaa
    14521 tcacatctaa atagccattt tttctcgcaa gttcctcttg ttctcttctt aatactagtt
    14581 caagaattgc atcaacttga taattatact ctatcggcaa tgctccccaa ccgcctttaa
    14641 agtaaatatt gagagcatct tgatcgccat aagttaagtt tacaattttt tcattgatag
    14701 taaccaactc atcacatact gaatacttct tccaaagtat attattaatt aatagtaccc
    14761 cagaattaaa ataagggctg aggtctaaat caagataagc cggtatatga ttatgaacat
    14821 aatcctctac tgcatataaa ggtataccat taaaattaag ccagtacatt tctgataaat
    14881 tatcatcaac cacaatatct gaatctaaat atagtacacg ttcttcgtcc agatatggaa
    14941 taaggtaacg ataaaaaccc gtttcattaa tatgctctaa actcttatat tttgaa
//
LOCUS       DANLRK010000048        14756 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.048, whole genome shotgun sequence.
ACCESSION   DANLRK010000048 DANLRK010000000
VERSION     DANLRK010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 14756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14756
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.048"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(<1..464)
                     /gene="sixA"
                     /locus_tag="PXA33_001051"
     CDS             complement(<1..464)
                     /gene="sixA"
                     /locus_tag="PXA33_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HDL5120392.1"
                     /translation="MNIWIMRHGEAGFNASSDAARCLTDNGIKNTISQGKWLGEHLAN
                     QQIQLDKILVSPYLRAQQTLENVLIGMQAVNFPQHFTNLIESWDEVTPDGNPHTVESY
                     LHFLREEGAKNILLISHLPLVFDLVQILTNNQSNVQFPTSTIAEINWVKTKA"
     gene            591..2030
                     /gene="pyk"
                     /locus_tag="PXA33_001052"
     CDS             591..2030
                     /gene="pyk"
                     /locus_tag="PXA33_001052"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005818583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HDL5120393.1"
                     /translation="MSRKLRRTKIVCTMGPATDRGNTLEKIIAAGANMVRMNFSHGIP
                     EDHIERANKVREIAARLGRHVAILGDLQGPKIRVSTFKDGKIFLNIGDKFTLDADLPR
                     GEGHQEAVGLDYKNLPNDVVPGDILLLDDGNVQLKVLEVEGVKVHTEVTVGGPLSNNK
                     GINKLGGGLSAPALTDKDKEDIKLAAKIGVDYLAVSFPQSSADLHYARELAKEAGLEA
                     KIVAKVERAETVATEEAMDDIILGADVVMVARGDLGVEIGDAALVGVQKRLIRRARKL
                     NRVVITATQMMESMIKKPMPTRAEVMDVANAVLDGTDAVMLSGETANGDYPVETVKSM
                     AEVCLGAETMPSINISRHRMEGTFKSIEEAVAMSAMYTANHLEGVSAIISLTHSGETA
                     KLTSRISSGLPIYAMSRNQKALNLCALYRGVTPVYYNEDSRTMEGAKKAIAQLKEQGY
                     LLTGELVLLTHGDELKAGGTNTCRILRVE"
     gene            2126..3163
                     /locus_tag="PXA33_001053"
     CDS             2126..3163
                     /locus_tag="PXA33_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HDL5120394.1"
                     /translation="MHNKLLSICILRLSAIGDVCHTLAVIQAIQRKYPSAEITWIIGK
                     TEAYLMKDIPNVHLIPYDKKIGWKGIFTLWQKLSHKRFDFLLNLQTAFRASILSLGIK
                     AKKKIGFNQDRAREMQWLFTNQKVEMTDSPHVLDGQMMFAKAIGIMDLSAQWNLPVSD
                     EDLAYGKQFIDFTRKNILIAPCSSKKEKDWLPERYAEIANWLSKQNINVLIAGSPSQY
                     EMETAAKIQQLAPNCTSIAGKTTLKQLAGVIRQVDLVISSDSGPAHIATTQNTPVIGL
                     YAIHNPRRTGPYNDLDKVVSVYDEAILQSYGKPWQQLAWATKAKGKNWMEKITVESVK
                     QKVVETLKITL"
     gene            3203..4261
                     /locus_tag="PXA33_001054"
     CDS             3203..4261
                     /locus_tag="PXA33_001054"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HDL5120395.1"
                     /translation="MNTVFNQDSVHNVNIQDEKVLLTPQELKQEFPLPEHLRNQIERS
                     RKTISDIIHRRDKRQLIVIGPCSIHDPVAALEYGKKLKALSEQVSDKLYIVMRVYFEK
                     PRTTVGWKGLINDPKMDGSFDVETGLRIARKLCLDLAELGLPLATEALDPMTPQYLAD
                     LFSWSAIGARTTESQTHRELASGLSMAVGFKNGTDGDLAVALNAMQSSAQSHSFIGIN
                     QQGQVTLLKTKGNADGHIILRGGKTPNYEKQYVDDCEWVLKQANLPTAIMIDCSHGNS
                     NKDYRRQPLVAENVLEQLLAGNTSIIGLMIESNLHAGNQSSELPFEQMKYGVSITDAC
                     IDWQTTEELLTHFAEKLR"
     gene            4316..5437
                     /gene="tyrA"
                     /locus_tag="PXA33_001055"
     CDS             4316..5437
                     /gene="tyrA"
                     /locus_tag="PXA33_001055"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HDL5120396.1"
                     /translation="MNPLAPLREQIDQVDQQLIALLSERLALVAEVGKVKSEHGIPVY
                     APERETAMISARRSEAEKQGIPADLIEDVLRRIMRESYANENKHGFKRVNSDIQKIVI
                     VGGKGKLGGLFGNYLTLSGYNVEALGRDDWDNAEQILANADVVIVCVPIANTLATIER
                     LQSYLTENMILADLTSVKHQPLQKMLEVHNGAVVGLHPMFGPDIASQAKQVVACCHGR
                     FPERYQWLLEQIKIWGAKIEEIEAAAHDNAMTYIQALRHFSTFAFGLHLSRQPVQLSQ
                     LLALSSPIYRLELAMIGRLFAQDGSLYADIIADKPENLATIETLKESFEQGLDFFKRN
                     DKAGFIKAFEEVHHWFGDYSEQFLKESRVLLQQAHDSRK"
     gene            complement(5628..6713)
                     /gene="recF"
                     /locus_tag="PXA33_001056"
     CDS             complement(5628..6713)
                     /gene="recF"
                     /locus_tag="PXA33_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HDL5120397.1"
                     /translation="MALTRLLINHFRNIQHTDLAFSPHFNFLVGANGSGKTSLLESIF
                     YLGHGRSFKSHISNRIIHYDKDDFVLHGKIDEAKHSWSVGIQKFRSGETTLKINGEDG
                     NKIADLAHLLPMQVITPEGLTLLNGGPSFRRAFLDWGLFHQHPDFYAHWNNLRRLLKQ
                     RNSALQQVRSYQELKAWDIELVKTTYAVSEMRAEYAEALRPEIEKTCQFFLPEIEIGL
                     SFHQGWEKGADYAEILAQGFERDKALGYTMIGAQKADFRFKANGLPVEDVLSRGQLKL
                     LMCALRLAQGEHLVAQKQRQCLFLIDDFASELDPTKRELLAHRLRESGSQVFVTAITQ
                     DQLNQMQWQDRSDDCLFTVKNGEIQPQ"
     gene            6853..7182
                     /locus_tag="PXA33_001057"
     CDS             6853..7182
                     /locus_tag="PXA33_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HDL5120398.1"
                     /translation="MFGKGGLGGLMKQAQQMQERMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRIEIDPSLMEDDKEMVEDLVAAAFNDAVRRAEEMQKEKMASVTAGMQLP
                     PGMKFPF"
     gene            7190..7795
                     /gene="recR"
                     /locus_tag="PXA33_001058"
     CDS             7190..7795
                     /gene="recR"
                     /locus_tag="PXA33_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015939588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HDL5120399.1"
                     /translation="MQTSPLLENLMEALRALPGVGPKSAQRMAYHLLQRNRSGGVALS
                     KALNEAMSHIGHCKSCRTFTEEEECTICKNPRRQMSGQLCVVEMPEDIQAIEQTGQFS
                     GRYFVLMGHLSPIDGIGPREIGLDLLQQRLEQESFHEVILATNPTIEGDATANYIAEM
                     CRMYNVKATRIAHGVPVGGSLEMVDGTTLSHSFAGRRDIDI"
     gene            7867..8193
                     /gene="grxD"
                     /locus_tag="PXA33_001059"
     CDS             7867..8193
                     /gene="grxD"
                     /locus_tag="PXA33_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005705415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HDL5120400.1"
                     /translation="METIDKIKQQISENPILLYMKGSPKFPSCGFSARAVEAVINCQV
                     PFGYVDILTNPDIRAELPKFANWPTFPQLWVEGELVGGCDIVLEMLQKGELQTLLKET
                     AAKHAA"
     gene            8335..9372
                     /locus_tag="PXA33_001060"
     CDS             8335..9372
                     /locus_tag="PXA33_001060"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HDL5120401.1"
                     /translation="MALKELDWANLGFSYIKTDYRFIAHWKDGKWNEGELTQDNTLHI
                     HEGSTALHYGQQCFEGLKAYRCKDGSINLFRPDQNAERMQRTADRLLMPRVPTELFIR
                     ACKKVVKANQDWLGPYGSGATLYLRPFLIGVGENIGVKAAPEFIFSVFCCPVGAYFKG
                     GLAPSNFITTDYDRAAPMGTGGVKVGGNYAASLLPHELAVEQSSTTRKFADAIYLDPK
                     THTKIEEVGAANFFGITKDNKFITPISESILPSITKYSLLHLAQERLGMEAIEGDVYI
                     DQLDQFAEAGACGTAAVITPVGGIQHKDKFHVFYSETEVGPVTRRLYAELTGIQFGDV
                     EAPQGWIVKVE"
     gene            9399..9947
                     /locus_tag="PXA33_001061"
     CDS             9399..9947
                     /locus_tag="PXA33_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane lipoprotein lipid attachment
                     site-containing protein"
                     /protein_id="HDL5120402.1"
                     /translation="MAKLFANSTACLYLENMKKLIFLGLSALALAGCSSTISNPNQLI
                     GQWQCTIEYDDFNIRTVDNLRFSTNGTMTNQGGIYYPIQKSIFEYSLQQNGRWSVKNG
                     AIVYRIVSESVQRSHRSQIWSELQRDAELQQFEENLFSSLSDSDNNKTVELIITDFDE
                     KEMDIKQEIKGHKAYKGQCIKQ"
     gene            9956..10300
                     /locus_tag="PXA33_001062"
     CDS             9956..10300
                     /locus_tag="PXA33_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDL5120403.1"
                     /translation="MIQRFEVGSRFSEMAIHNGVAYLAGQVPLDDTADAYTQTQQVLA
                     EIDKWLAKANSDKSKILMATVYLKEMADYAEMNRAWDEWVAPNNAPPRAAVEAKLANP
                     NWKVEIVVTAAV"
     gene            complement(10333..12111)
                     /locus_tag="PXA33_001063"
     CDS             complement(10333..12111)
                     /locus_tag="PXA33_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HDL5120404.1"
                     /translation="MSTLDFHFIHRFHQQVQALSHHPALRFMQDEKWSEISWLELQKQ
                     VNNLSYALLANGLAVQEKIAIFAQNMPRWTITDIAAMQIRAVAVPIYATSTTKQLEYI
                     LNDGDIKILFVGDQEQYDQAIEIANNCSLLIKIVAMKDSIDLRDFPIAQHWQDFIKVN
                     QDEAELTKRLNDKCLEDLFTLIYTSGTTGEPKGVMLDYANLAHQLQAHNKALPHIDEN
                     DISLSFLPLSHIFERAWVTYVLYRGAVNCYLEDPQQVRQALSTVRPTLMCAVPRFYEK
                     IYTAVWDKVQQAPIHRRALFKWAIRTAEDNLFGKKSAFFHRLQYIMADKLVLGKLRAL
                     LGGRIRMMPCGGAKLDPSIGLFFQSIGINVKLGYGMTETTATISCWQEKGFNINSTGT
                     LMPDVEVKISEENEILVRGGLVMKGYYKKPEATAQAFTAEGFLKTGDAGELDSEGNLY
                     ITDRIKELMKTSNGKYIAPQYIEGKIGKDKFIEQIAVIADAKKYVSALIVPCFDSLEE
                     YAKKLNIRYQDRLELIKHYEVVRMFEQRINELQKDLASFEQIKKFTLLSQAFSTKMEE
                     ITPTLKLRRKVILERYKQQIEKMYES"
     gene            complement(12337..12906)
                     /locus_tag="PXA33_001064"
     CDS             complement(12337..12906)
                     /locus_tag="PXA33_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005821628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HDL5120405.1"
                     /translation="MNQENHKIEDIIRIFNQCFSDEYNTRLERGGNYPIYLPEFMDED
                     GVPSERPYNVILFAHGYYSSALHEIAHWLVAGEARRKLEDFGYWYEPDGRSAERQREF
                     ESAEIKPQAIEWVLATAAGFRYFASADNLSGNPGDNSAFKQAVYDQVKLYAERGYLPK
                     RAETLRKALCAFYGTPDVIDLAQFDIERI"
     gene            complement(12916..14679)
                     /locus_tag="PXA33_001065"
     CDS             complement(12916..14679)
                     /locus_tag="PXA33_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HDL5120406.1"
                     /translation="MIRYFFSLVLSLLMINSSHANLFSQPKFLKSEEAFVFSIQNENE
                     TLQLNWDIADNYYLYKKEIRVTPQNIEIGDITFPAAEQYNDEFFGQVEIYRNHLGLKV
                     PFKEIKDNASIVVEYQGCTKGFCYPPERVELKLDSILSATPEELAQLEAQAVEKSENF
                     AKTDNMAPPENVAKAEQDQLAENLANNRFSIFWFFVLGVGLAFTPCVLPMLPLLSAIV
                     IGNKQRPSTAKALLLSIAYVQGMAVTYTLLGLIVAAIGLPFQIALQSPPVLIGLSIVF
                     ILLACSMFGLYEIQLPNSWQQKLNAMSQKQQGGALGSVFLMGMIAGLIASPCTSAPLS
                     GALLYVAQSGDLVTGGLALYLLALGMGLPLILITLFGNKILPKSGEWLLKVKAAFGFV
                     MLALPVFLLSRVLPSHYEPFLWSALAVAFLIWLVDAIPSNSITKKIIKVLLLLALAIA
                     AKPWADLAWNGSTTQQQTTPHLALEKIQSLADLETKLTASKGKKVMLDLYADWCVACK
                     EFEKYTFSDQNVQNKLNEMVVLQIDMTKNSAENIELMKHFNVLGLPTILFFDENGNEM
                     SQSRVTGFLDAEQFLAWLNKL"
BASE COUNT         4438 a         2850 c         3195 g         4273 t
ORIGIN      
        1 tcagcttttg tctttaccca attaatttct gctatggttg aagttggaaa ttgtacattg
       61 ctttggttat tcgttaaaat ttggacgaga tcgaacacaa gtgggagatg tgaaatcaga
      121 agaatatttt ttgcgccttc ctctcttaaa aaatgtagat agctttctac tgtatgcggg
      181 tttccgtcag gcgtaacctc gtcccaactt tcaatgaggt tggtaaaatg ttggggaaaa
      241 ttgaccgctt gcataccaat aagcacattt tcaagcgttt gttgcgccct aaggtatggg
      301 ctgacgagaa ttttatctaa ttgaatttgt tggttggcta aatgttctcc taaccatttt
      361 ccctgtgaaa tggtgttttt tataccattg tcggttaaac agcgagcagc atcactcgaa
      421 gcattaaagc ctgcttcccc atggcgcata atccaaatat tcataacatt ctcctttagt
      481 tgtatttaaa ctctcttgat ttaggcgtat aatgaccctc gtttaacgca taatcaagat
      541 gcttagaaag attttaactt aatttttaaa tttattgatg gagtcaaata atgtctagaa
      601 aactcagaag aacgaagatc gtttgtacaa tgggacctgc aacagatcgt ggcaatacgt
      661 tggaaaaaat cattgctgcc ggtgcaaata tggttcgtat gaatttctct cacggtattc
      721 ctgaagatca catcgaacgt gcgaataaag tgcgtgaaat tgctgcaaga ttaggtcgcc
      781 acgttgcgat tttaggcgat ttgcaaggtc ctaaaattcg tgtttcgaca tttaaagacg
      841 gtaaaatttt cttaaatatt ggtgataaat tcaccttaga cgcagattta ccgcgtggag
      901 aaggccatca agaagctgta ggtttagatt acaaaaactt accaaatgat gtagtacctg
      961 gtgatattct attgttagat gacggtaatg ttcaactgaa agtgttggaa gtggaaggcg
     1021 ttaaagttca tactgaagtg acagttggtg gcccgttatc aaacaataaa gggattaaca
     1081 aattaggtgg tggtttatca gctcctgcat taaccgacaa agataaagaa gacattaaat
     1141 tagcggcgaa aatcggtgtg gattacctag cggtttcatt ccctcaatca agtgcagatt
     1201 tacattatgc acgtgaatta gcaaaagaag caggcttaga agcgaaaatt gtagcgaagg
     1261 ttgaacgtgc cgaaactgtt gcgactgaag aagcaatgga cgacatcatt ttaggtgcgg
     1321 acgttgtgat ggttgctcgt ggtgacttag gggttgaaat tggtgatgcg gctttagtgg
     1381 gtgtgcaaaa acgtttaatc cgccgtgctc gtaagctaaa tcgtgtggta attaccgcaa
     1441 cccaaatgat ggaatcgatg attaaaaaac caatgccgac ccgtgctgaa gtgatggatg
     1501 ttgcaaatgc tgtgttagat ggtactgatg cggtaatgct ttccggcgaa actgcaaacg
     1561 gtgactaccc tgttgaaacg gtaaaatcaa tggcagaagt gtgcttaggt gctgaaacaa
     1621 tgccaagcat taacatttct cgccaccgta tggaaggtac gtttaaatct atcgaagaag
     1681 ctgtggcaat gtctgcaatg tacaccgcaa accatttaga aggtgtatcg gcaattattt
     1741 cattaactca ttcaggcgaa acggctaaat taaccagccg tatcagctca ggtttaccga
     1801 tttatgcgat gtctcgtaac caaaaagcat taaacctttg tgcgttatat cgtggtgtaa
     1861 caccggtata ttacaatgaa gacagccgca caatggaagg ggcgaaaaaa gccattgctc
     1921 aattaaaaga acaaggctat ttattaaccg gcgaattagt gcttttaact cacggagatg
     1981 aattaaaagc tggcggtaca aatacttgtc gtatcttaag agttgaataa tctaaacgat
     2041 taacgcaaaa gccacataaa acactttatg tggctttttt tctgtcttaa tatagcttaa
     2101 tgtaatctga actattgata ttattatgca caataaactt ctttctatct gtattctccg
     2161 cctttctgcc attggcgacg tttgccatac cttagcggtt attcaagcta ttcaacgaaa
     2221 atatcctagt gctgaaatta cttggattat cggcaaaacg gaagcgtatt taatgaaaga
     2281 tattccgaat gttcacttaa ttccttatga taaaaaaata gggtggaaag gtatctttac
     2341 actttggcaa aaactttctc acaagcggtt tgatttttta ttaaatttac aaacggcatt
     2401 ccgagcttca attctatcgc ttgggattaa agcaaagaaa aaaatcggct ttaatcaaga
     2461 cagagcaagg gaaatgcaat ggttgtttac caaccagaaa gtagaaatga cagactctcc
     2521 tcacgtgctt gacgggcaaa tgatgtttgc caaagccatt gggataatgg atttatcggc
     2581 acaatggaat ttacctgtga gtgatgaaga tttagcttat ggcaaacaat ttattgattt
     2641 tacaaggaaa aatatactca ttgctccttg ttcaagcaag aaagagaaag attggttgcc
     2701 ggaacgctat gcagaaattg ctaactggct aagtaagcaa aatatcaatg tattaattgc
     2761 aggttctcca tcgcaatatg aaatggaaac cgcagccaaa attcaacaac ttgccccgaa
     2821 ttgtacgagt attgccggta aaaccacgct aaaacagctt gcaggtgtta ttcgtcaggt
     2881 tgatttggtg atctcttcag attcaggtcc tgcccatatc gccactacgc aaaatacgcc
     2941 ggtgattggg ttgtatgcga ttcataatcc aagacggaca ggcccttaca atgacttaga
     3001 caaagtggtt tcggtatatg atgaagcgat tttgcaatct tatggtaaac cttggcagca
     3061 attagcttgg gcaactaaag ctaaaggcaa aaattggatg gaaaaaatca cggtggagag
     3121 tgtgaaacaa aaagtagttg aaactttaaa aattacgttg taaagtttgt gtattaaaag
     3181 atttacacaa aaggaaatta aaatgaacac agtgtttaat caagatagcg tgcataacgt
     3241 aaatatccaa gatgaaaagg tattactgac cccacaagag ttaaaacaag aatttccgtt
     3301 gccggaacat ttacgcaatc aaattgaacg ttcacgcaaa acgatttccg atattattca
     3361 cagacgagat aaacgccaac tgattgtgat tggaccttgt tcgattcacg acccagttgc
     3421 tgcccttgaa tatggtaaaa aattaaaagc tctttcggag caagtgagtg ataagttgta
     3481 tattgtgatg cgggtttatt ttgaaaagcc ccgcactact gtaggttgga aagggctgat
     3541 taacgatccg aaaatggacg gctcttttga tgtcgaaacc ggcttacgta tcgcacgaaa
     3601 actctgtttg gatttagccg aacttggctt gccgttagcg accgaagcct tagatccgat
     3661 gacaccacaa tatttagcgg atttgttcag ctggtcggca attggagcaa gaacaactga
     3721 gtctcaaact caccgtgaat tagcctcagg tttgtcaatg gcggtaggct ttaaaaatgg
     3781 tacagatggt gatttagccg ttgcacttaa tgcgatgcaa tcttccgctc aaagccatag
     3841 ctttatcggc attaaccaac aggggcaagt tactttactc aaaacaaaag gtaatgcaga
     3901 cggacatatt attttacgtg gcggtaaaac cccaaattat gaaaagcaat atgttgatga
     3961 ttgtgaatgg gtgctaaaac aagcaaactt acctacagcg attatgattg attgcagcca
     4021 cggcaattcc aataaagatt accgccgcca accgctggtg gcagaaaatg tattagagca
     4081 gctacttgct ggcaatactt cgattatcgg tttaatgatt gaaagcaatt tacacgcagg
     4141 taatcaatca tcagaattgc cgttcgagca gatgaaatac ggtgtttcta ttacggatgc
     4201 ttgtattgac tggcaaacta cagaagagct tttaactcac tttgctgaaa agttgcgtta
     4261 aagataattt gcaaaaaatt gcataaaaac gaccgcttgt aaattggtaa caaatatgaa
     4321 cccattagcc ccattacgag aacaaattga tcaagttgat caacaactta tcgccttact
     4381 ttctgaacgc ttagctcttg tggcagaagt tgggaaagtc aaatcagaac acggcattcc
     4441 tgtttatgct ccggagcgag aaaccgcaat gatttccgcc cgccgctctg aagcggaaaa
     4501 acaaggtatt ccggcggatc tcattgaaga tgtgctacgc cgtataatgc gtgaatctta
     4561 tgccaatgaa aacaaacacg gcttcaaaag agttaattcg gacattcaaa aaatcgtgat
     4621 tgttggtggt aaagggaaat taggtgggct gtttggcaat tatttaaccc tatccggcta
     4681 taatgttgag gcattaggac gtgatgattg ggacaatgcc gagcagattt tagccaatgc
     4741 agatgtggtt attgtgtgtg tgccgattgc caacacccta gcaaccatcg agcgtttaca
     4801 atcttattta accgaaaata tgattttggc ggatttaacc tccgtcaaac atcagccgtt
     4861 gcaaaaaatg ctggaagtgc ataatggagc ggtagtcggt ttacacccga tgttcggacc
     4921 ggatattgca agtcaggcaa agcaagtggt cgcttgctgc cacgggcgtt tccctgaacg
     4981 ttatcaatgg ttgcttgagc aaatcaaaat ttggggggca aaaattgaag agattgaggc
     5041 agcagcacac gataatgcaa tgacctacat tcaagcccta cgccactttt ccacttttgc
     5101 attcgggcta catctttctc gccagccggt gcaactctct caattattgg cactatcttc
     5161 gccgatttac cgcttagagt tggcaatgat tgggcgttta tttgcccaag acggcagctt
     5221 atatgccgat attattgccg ataaaccgga aaatttagcc accatcgaaa ccttaaaaga
     5281 gagttttgaa caagggctag atttctttaa acgcaacgac aaagccggtt ttatcaaggc
     5341 ttttgaggaa gtgcatcact ggtttggcga ttattccgag cagttcttga aagagagtag
     5401 agtattgctc caacaggctc acgattcaag aaagtaaagg taggttgagg ttttgctctg
     5461 atagcactag cgtccacgct agtgctattt aatattctac aagcggttaa taaagagctg
     5521 ttttttgcaa atttttatgc aaaattatcc gcttgttttg gcacgagcgt ggacgctcgc
     5581 gccatcggaa gaaacatttt tgtaaaattg cccccaaatc tgaccgctta ctgtggctga
     5641 atttctccat tttttaccgt aaacaaacaa tcatcgcttc tgtcttgcca ttgcatttga
     5701 ttgagctgat cttgagtaat ggcggtaacg aatacttgtg agccactttc tcttaaacga
     5761 tgtgctaaaa gctcacgttt ggttgggtcg agttcggagg caaaatcgtc aattaaaaat
     5821 aggcattggc gttgtttttg ggcgaccaaa tgttcgcctt gagccagtct taacgcacac
     5881 atcaatagct ttaattgccc acgggaaagc acatcttcca ccggtaagcc gttcgcttta
     5941 aaacggaaat cggctttttg agctccaatc atcgtatagc caagagcttt atcccgctca
     6001 aaaccttgtg ccagaatctc ggcataatcc gccccttttt cccagccttg atgaaagctc
     6061 aagccgattt cgatttccgg caggaaaaat tggcaggttt tctcaatttc ggggcgtaac
     6121 gcttcggcgt attcggctcg catttcgctc acggcataag tggttttcac caattcgata
     6181 tcccacgctt tgagttcttg ataagagcgg acttgttgca aagccgaatt acgttgcttt
     6241 aataaacggc gaagattgtt ccaatgagcg tagaaatcag ggtgctggtg aaataagccc
     6301 cagtccaaaa aagcacggcg aaagctcgga ccaccgttaa gtaaggttaa gccttcgggg
     6361 gtgatcactt gcatcggcaa taaatgggct aaatcggcaa ttttgttgcc gtcttcaccg
     6421 ttgattttaa gtgtcgtctc gccactgcga aatttctgaa tgccgaccga ccaactgtgc
     6481 tttgcctcgt caattttacc gtgtagtacg aaatcgtctt tatcgtaatg gataatgcgg
     6541 ttgctgatat ggcttttgaa tgaacgcccg tgtccaagat agaaaattga ttccaacaaa
     6601 ctggttttgc cacttccgtt cgccccgact aaaaaattaa aatgcgggct aaatgctaaa
     6661 tcggtatgtt ggatatttct aaaatggttg attaataaac gggttaaagc catagttaaa
     6721 gtgcgaggaa taaaaagagt agccatttta gcaaatttgc aaaattttgg gaaaaattaa
     6781 ccgcttgcgg attagaaagt ggttcagtag aataagccgt ttatttttta ttgaaaatta
     6841 agaggaaaaa ttatgtttgg aaaaggtggc ttaggcggct taatgaaaca ggctcagcaa
     6901 atgcaagagc gtatgcaaaa aatgcaagaa gagatcgccc aattagaagt aacaggcgag
     6961 tctggtgcgg gtttggtaaa agtcactatt aacggggctc acaattgccg ccgaattgaa
     7021 attgatccat cattaatgga agatgataaa gagatggtgg aagatttagt tgccgctgcc
     7081 tttaacgatg cggttcgccg tgcagaagaa atgcaaaaag agaaaatggc aagtgtgact
     7141 gccggtatgc aactgccgcc gggtatgaaa ttcccattct aatttaagta tgcaaaccag
     7201 tcctttactt gaaaatttga tggaagcctt gcgtgcattg cccggtgtcg gaccgaaatc
     7261 ggcacagcga atggcttacc atctgttgca acgtaatcgt tccggcggtg tggcgttgtc
     7321 aaaagcctta aatgaggcaa tgagccatat cggacattgc aaatcgtgcc gtacttttac
     7381 cgaagaggaa gagtgtacga tttgtaaaaa tcctcgccgt cagatgagcg ggcaactttg
     7441 tgtggtagaa atgccggaag acattcaagc gattgagcaa accgggcaat tttcagggcg
     7501 ttattttgtg ctgatggggc atttgtcgcc gattgatggt ataggccctc gtgaaatcgg
     7561 cttagattta ttgcaacaac gcttggaaca ggaatctttc cacgaagtga ttttggcgac
     7621 taatccgacc attgaaggtg atgctaccgc aaactacatt gctgaaatgt gcagaatgta
     7681 taacgtgaaa gctactcgta ttgctcacgg tgtgccggtc ggtggttcat tagaaatggt
     7741 tgatggcaca acgctttctc actcttttgc aggacggcgt gatattgata tttaaagcta
     7801 tttctgtgct aacttaattc tgatataatt actcaactat tttaaattat tattttgagg
     7861 ctaaaaatgg aaactattga caaaatcaaa caacaaatca gcgaaaatcc gattctactt
     7921 tatatgaaag gttcaccgaa attcccatct tgtggtttct ccgctcgtgc ggtggaagcg
     7981 gtaattaact gccaagtgcc tttcggttat gtggatattt taaccaatcc ggatattcgt
     8041 gcggaattgc caaaatttgc aaactggccg actttcccac aattatgggt agaaggtgaa
     8101 ttagttggcg gttgtgacat cgtattagaa atgctccaaa aaggcgaact tcaaacctta
     8161 ttaaaagaaa ccgcagcaaa acacgcagca taatttcaaa ccagtatgtt aaggctctta
     8221 tgtttaagag ccttaatttt ttcttgcgat ttaggcttaa acttcttacc tcaagagcta
     8281 ttttgtttta gaatgtcaaa attatttcgt aacataaaaa taaaaggaca caacatggca
     8341 ttaaaagagt tagattgggc aaatttaggc ttttcttata tcaaaaccga ttaccgtttc
     8401 attgcacatt ggaaagacgg aaaatggaat gaaggcgaat taactcaaga caatacactg
     8461 catattcacg aaggctcaac tgccctgcac tatgggcaac aatgttttga ggggttaaaa
     8521 gcctatcgct gtaaagatgg ctcaattaac ttattccgcc cggatcaaaa tgccgagcgt
     8581 atgcaacgca ccgcagatcg tttattaatg ccacgtgtgc caaccgagct gtttattcgt
     8641 gcctgtaaaa aagtggtaaa agccaatcaa gattggttag ggccttacgg ttcgggggca
     8701 accctttatt tacgtccgtt tttaatcggc gtgggcgaaa atattggggt gaaagctgca
     8761 cctgaattta ttttttccgt attctgctgt ccggtggggg catatttcaa aggtggctta
     8821 gcaccctcaa acttcattac aaccgactat gaccgtgcag ccccaatggg aacaggtggt
     8881 gtgaaagtag gcggtaacta cgctgcgagt ttattaccgc acgaattggc ggttgagcaa
     8941 agttcaacta cccgtaaatt tgccgatgcg atttatttag atccgaaaac ccataccaaa
     9001 attgaagaag tgggggcggc gaacttcttt gggattacca aagacaacaa attcatcacc
     9061 ccgatttcag agtcgatttt gccaagtatt accaaatact cattgctaca ccttgctcaa
     9121 gagcgtttag gtatggaagc gattgaggga gatgtgtata tcgaccagtt agaccaattt
     9181 gccgaagccg gtgcttgtgg tacggctgcg gtgattacgc cggttggtgg tattcaacat
     9241 aaagacaaat tccacgtctt ctattcagaa accgaagtgg ggcctgtcac ccgccgttta
     9301 tacgcagaat taaccggtat tcaatttggc gatgttgaag caccgcaagg ctggattgta
     9361 aaagtagagt aattttacac aagtaacaag cggtcgaatt ggcaaaactt tttgcaaatt
     9421 caaccgcttg tctttattta gagaatatga aaaagttaat ttttttaggt ttatcagcac
     9481 ttgcattagc tggttgttcc tcaacaattt ctaatcctaa tcaactgatt gggcaatggc
     9541 aatgcactat tgagtacgat gattttaata ttcgtacggt ggataattta cgattttcca
     9601 caaatgggac tatgaccaat caaggtggta tatattaccc tattcaaaag tcgatttttg
     9661 agtattcatt acaacagaat gggcgctgga gtgtgaaaaa cggggctatt gtttatcgta
     9721 tcgtctcaga atctgtacag cgtagccatc gttcacaaat atggtctgaa ttacaacgtg
     9781 atgcggagct acaacaattt gaagagaatt tatttagctc actttctgat agtgataata
     9841 ataaaacagt ggaactaatc atcactgatt ttgatgaaaa agaaatggat atcaagcaag
     9901 aaataaaagg gcataaagct tataaagggc aatgtataaa acagtagagg taaaaatgat
     9961 tcaacgtttt gaagtaggta gccgtttttc ggaaatggca atacataacg gtgtggctta
    10021 cttggcaggg caagtgccgt tagatgacac cgccgatgct tacacccaaa cccagcaagt
    10081 gcttgcagaa attgataaat ggctggcaaa agccaacagc gataaaagta aaattctaat
    10141 ggctacggtt tatttaaaag agatggcaga ttatgcggaa atgaaccgtg cgtgggacga
    10201 atgggttgcc ccaaataacg ctccaccaag agcggcggtg gaggctaaat tagccaatcc
    10261 aaattggaaa gtcgaaattg tggttaccgc tgcggtataa atgaaaagca gaaagacgat
    10321 ctctgttttt cattatgatt cgtacatttt ttcgatttgt tgcttatagc gttctagaat
    10381 gacctttcgt cttaatttta atgtaggcgt aatttcttcc attttggtgc taaatgcttg
    10441 cgaaagtagt gtaaactttt tgatctgttc aaaacttgca aggtcttttt gtaattcgtt
    10501 aatgcgttgt tcaaacattc tgacaacctc ataatgttta atgagttcta atctgtcttg
    10561 atagcgaata ttgagctttt ttgcatactc ttcgaggcta tcaaagcaag gaactatcaa
    10621 cgcagaaaca tattttttgg catcagcaat caccgcaatt tgctcaataa acttatcttt
    10681 acctattttt ccttcaatgt attgtggggc aatatattta ccgtttgaag ttttcattaa
    10741 ttctttaata cgatcggtaa tataaagatt gccttccgaa tccagttcac cggcatctcc
    10801 cgtttttaga aaaccttcag cagtaaaggc ttgagcagtg gcttccggtt ttttgtagta
    10861 acctttcata accaagcctc cacgcactaa aatttcattt tcttcactaa ttttgacctc
    10921 aacatcaggc attaaggtac cggttgagtt gatattaaag cctttttcct gccagcatga
    10981 aatagtggca gtagtttcgg tcattccgta tccaagtttt acattgatgc ctatactttg
    11041 gaaaaatagc ccgatagacg gatctaattt agctcctcca cacggcatca tacgaatacg
    11101 accgcctagt aaggctcgta attttcctag caccaattta tctgccataa tgtactgcaa
    11161 gcggtggaaa aaggctgatt ttttaccaaa cagattgtct tcggcggtac ggattgccca
    11221 tttaaataac gctcggcgat gtatcggtgc ttgttggact ttatcccaca ccgcagtata
    11281 gattttttca tagaaccttg gtacggcaca cattaatgta ggtcgtaccg ttgagagtgc
    11341 ttgacgtacc tgttgaggat cttccagata gcaatttact gcccctctgt atagcacata
    11401 agtgacccaa gcacgttcaa agatatgcga taaaggtaaa aatgaaaggg atatatcatt
    11461 ttcgtcaata tgagggagtg ctttattatg tgcctgaagt tgatgagcta aattagcata
    11521 atctaacata acacctttag gctcaccggt tgttcccgag gtataaatta aagtgaataa
    11581 atcctctaag catttatcat ttaaacgttt ggttaattca gcttcatctt gattaacctt
    11641 aatgaaatct tgccaatgtt gagcgatagg gaaatcacgc agatctattg agtctttcat
    11701 cgcgacaatt tttattaata gcgaacagtt gttcgctatt tctatggctt gatcatattg
    11761 ttcctgatcg ccaacaaaga ggatttttat atccccgtca ttcaggatat attcaagttg
    11821 tttggtggta ctggttgcat aaatcggcac tgctaccgca cgaatttgca ttgcagcaat
    11881 atcggtaatt gtccaacgag gcatattttg tgcaaaaata gcgattttct cttgaacggc
    11941 aagaccgttt gccagcaagg cataagaaag attattgacc tgtttttgta attctaacca
    12001 acttatttct gaccattttt catcttgcat aaaacgtaaa gctgggtggt ggctaagagc
    12061 ctgaacttgc tggtggaaac ggtggataaa gtgaaaatct aaagtgctca taatcttatt
    12121 tttactttct atttcagtga acagttgtac gctaatataa tagaaaatat gaaaaaaaga
    12181 aacagttgta ctctgaaaca aagggttttg gataggaagt aatcaaggca atagaaatag
    12241 ataagtaggg tatcgtttag tacaaatgca atatttttca aaaatgagac cgcttgtata
    12301 tgcaacaagc ggtctgtttt gttaaaaatt ttgcaactaa attcgttcaa tatcaaattg
    12361 agctaaatca atcacatcag gtgtaccgta aaaagcacag agggctttac gcaaggtttc
    12421 agctcgtttg ggaaggtagc cacgttctgc atagagcttg acttggtcgt acactgcttg
    12481 tttaaaggct gaattatcgc cgggattgcc cgagagattg tcggcactag caaaataacg
    12541 aaaaccggca gcagttgcta acacccattc aatcgcttgc ggtttgattt ccgcactttc
    12601 aaattcacgt tggcgttcgg cagagcgtcc gtccggctcg taccaatagc caaaatcttc
    12661 aagtttacgc cgagcttctc cagcaacgag ccaatgggca atttcgtgca aggcactgct
    12721 gtaatagccg tgagcaaata aaatcacatt gtaagggcgt tcgctcggca caccatcttc
    12781 gtccataaat tcgggcaagt aaatcggata attacccccc cgctctaggc gagtgttata
    12841 ttcatcagaa aagcattgat taaaaatgcg aataatgtct tcaattttat gattttcttg
    12901 attcatttct tttctttata acttattcag ccaagctaaa aattgctcgg catccaaaaa
    12961 gccagtaaca cgagattggc tcatttcatt gccgttttca tcaaaaaaca gaatcgttgg
    13021 caaacctaat acattaaagt gtttcatcag ctcgatattt tcagccgagt ttttcgtcat
    13081 atcgatttgc aacactacca tttcattcag tttgttctga acattttggt cactaaaggt
    13141 gtatttttca aactctttgc aggcgacaca ccagtcggca tagagatcga gcatcacttt
    13201 tttacctttg gaagcggtca attttgtctc taaatcggca agtgattgaa ttttttctaa
    13261 cgctaagtgc ggtgtagttt gctgttgagt ggttgagccg ttccaagcta aatctgccca
    13321 aggtttagcg gcaatggcta aagcgagcaa taataagact ttgatgatct ttttcgtgat
    13381 tgaattagat ggaatcgcat ccaccaacca gatcaaaaat gcaaccgcaa gggccgacca
    13441 taaaaacggt tcatagtggc ttggcagcac acggcttaat aagaacaccg gtaaggcaag
    13501 cataacaaaa ccgaatgcag ctttcacttt taacagccat tcgccggatt ttggcagaat
    13561 tttgttgccg aataaggtaa tcaaaatcag cggtaagccc attcccagtg cgagtagata
    13621 gagggctaag ccaccggtga ctaaatcgcc actttgtgcc acataaagca acgcaccgga
    13681 aagtggggca gaagtacaag gggaagcaat taagcctgcg atcatcccca ttaaaaacac
    13741 gctacccaac gcaccacctt gctgtttttg gctcattgca ttgagttttt gttgccagct
    13801 atttggtagc tggatttcgt ataagccaaa cattgagcag gcaagcagaa taaagacgat
    13861 agataaacca atcaacaccg gtgggctttg taaggcgatt tggaaaggca agccgatagc
    13921 cgctacaatt aagccaagta aggtgtaagt aactgccatt ccttgcacat aggcaatact
    13981 taacagcaat gctttagcgg tagaagggcg ttgcttattt ccaattacaa tcgcagagag
    14041 aagtggtagc atcggcagta cgcaaggagt gaatgctaat ccaacgccta atacaaaaaa
    14101 ccaaaaaatg gaaaaacgat tgttagctaa attttcagcc agttgatctt gctctgcttt
    14161 tgccacattt tcagggggcg ccatattgtc ggtttttgca aaattttcgc tcttttcgac
    14221 cgcttgtgct tccagttgcg ccagttcttc aggtgtagca gagagaatac tgtctaattt
    14281 aagctcaacc cgttccggtg gatagcaaaa gcctttggta cacccttgat attccactac
    14341 gattgaggcg ttgtctttaa tttctttaaa cggtaccttt agccctaagt ggttgcgata
    14401 gatttccacc tgcccgaaaa attcatcgtt atattgctcg gcagcgggaa aagtgatgtc
    14461 gcctatctca atattctgtg gcgtgacacg gatttctttt ttataaagat aataattgtc
    14521 tgcaatatcc caatttaact gtaatgtttc attttcattt tggattgaaa aaacaaaggc
    14581 ttcttccgat tttaggaatt tgggttgaga gaaaagattt gcgtgagatg agtttatcat
    14641 caataaactc aagacgagtg aaaaaaagta tctaatcata ttgaaatcct atttttaatt
    14701 gtctcggcga aaattatagc gtaatcagta ttcatattcc aaataaatta cgccat
//
LOCUS       DANLRK010000049        14545 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.049, whole genome shotgun sequence.
ACCESSION   DANLRK010000049 DANLRK010000000
VERSION     DANLRK010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 14545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14545
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.049"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            70..411
                     /locus_tag="PXA33_001066"
     CDS             70..411
                     /locus_tag="PXA33_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HDL5120407.1"
                     /translation="MSSFQNWTEHTAHVKQSFGKLGQKHPKMLEAYKALGTAAAAEAL
                     DHKTRELIALAVAITTRCESCISVHAEEAAKAGATESEIAGALATAIALNAGAAYTYA
                     LRALEAVEEQK"
     gene            422..775
                     /locus_tag="PXA33_001067"
     CDS             422..775
                     /locus_tag="PXA33_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDL5120408.1"
                     /translation="MKKLAWILGFAAMMPMMSSAKQASVQQVQAEKAAGIWIDVRSAE
                     EFQQGHLNGAINVTHTEIAQRIAEVAPDKNQPIHLYCRSGHRAEAALNELKKLGYSNV
                     TNHGGYDDLIKAGLK"
     gene            835..1473
                     /locus_tag="PXA33_001068"
     CDS             835..1473
                     /locus_tag="PXA33_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005719747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgB family protein"
                     /protein_id="HDL5120409.1"
                     /translation="MSAFNNFVNFVANIVAPMQRQFINFVRIAICIVMVWIGGLKVCQ
                     YEADGIAHFVSNSPFFSYMYKKGPNLVDDGTGKMVMEYTLHKNPEGKMVAKNIEWHKE
                     NGTYTASYIIGATIVTVGLLTLSGIWFPVSGMAGGLLTFGMSIVTLSFMITTPEIWVP
                     NLGGDMPTPAHGFPYLSAVGRLIVKDVIMMAGGLVAAAECANRILARRKAIA"
     gene            1608..2789
                     /gene="purT"
                     /locus_tag="PXA33_001069"
     CDS             1608..2789
                     /gene="purT"
                     /locus_tag="PXA33_001069"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005615456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HDL5120410.1"
                     /translation="MTTIGTPLRTNATKVMMLGSGELGKEVVIELQRLGVEVIAVDRY
                     DNAPAQQVAHRAYTISMLDGNALRDLVEKEKPDFIVPEVEAIATATLVELEQEGYNVI
                     PTAKATQLTMNREGIRRLAAEELGLKTSPYRFVDNFEQFQQAIQEIGIPCVVKPIMSS
                     SGHGQSVIKSEADIQQAWDYSQQGGRAGGGRVIVEGFIKFDYEITQLTVRHIHGTSFL
                     APIGHIQVDGDYRESWQPQQMSDIALKKAQETAEKITSALGGRGIFGVELFVCGDEII
                     FNEVSPRPHDTGIVTMASQELSQFALHARAILGLPIPEIYRISPAASKAIVVEGKSDN
                     VRFGGVDKVLAEIGTNIRLFGKGEVNGHRRLGVILARDENTVRALEKARRAYDKLDIQ
                     L"
     gene            complement(2881..4374)
                     /locus_tag="PXA33_001070"
     CDS             complement(2881..4374)
                     /locus_tag="PXA33_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005556599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL5120411.1"
                     /translation="MFKQLQQVGKAFMLPIAILPAAGLLLGIGGALSNKATVQAYPIL
                     DNPTLQGIFQIMASAGSVVFGNLALLLCIGLGIGLAKRDKGVAALAAVVGYLIMTGTI
                     SALIPLFPPETKGIDTGVIGSLVMGLITVKLHNKYHNIQLPQILGFFGGSRFVPIVTA
                     FAAIFVGAVFFLIWPAFQQWLVSAGQGIAEMGEIGTFFYGFLMRLSGAVGLHHMIYPL
                     FWYTELGGTEVVNGETIIGAQKIFFAQLADPTHQGLFTEGTRFFAGRFDTMMFGLPAA
                     CLAMYHAVPKARRGAISGLFLGAALTSFITGITEPIEFMFLFVAPWLYVFHAFLDGVS
                     FYIADLLNISIGNTFSGGVIDFTLFGILQGNENTNWLRVIIVGLPWAALYYFSFLFLI
                     KKFNVMTPGRAEESEEVVEQQTSSLTENAHKIIAALGSANNIENVDACITRLRVAVKE
                     VKAVDKATLKKLGAIDVLEVGGGVQAVFGAKAVLYKSEVNQILGKED"
     gene            4575..6422
                     /gene="ilvD"
                     /locus_tag="PXA33_001071"
     CDS             4575..6422
                     /gene="ilvD"
                     /locus_tag="PXA33_001071"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012055207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HDL5120412.1"
                     /translation="MPILRSATSTQGRNMAGARALWRATGMKENDFGKPIIAVVNSFT
                     QFVPGHVHLKDMGQLVAAEIEKAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRDLIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMAAMRLNIPTIFVSGGPMEAGKTKLSDQLI
                     RLDLVDAMIESADPNVSDERIDAIERSACPTCGSCSGMFTANSMNCLTEALGLSLPGN
                     GSMLATHADRKELFLRAGREIVELCRKYYQQDDESVLPRSIATFDAFENAMSLDIAMG
                     GSSNTVLHLLATAQEAGVDFKMEDIDRLSRVVPCLSKIAPNTNKYHMEDVHRAGGIMG
                     LLGELDRAGLIHRHVKTVLGLTIEEQLNQYDIIRNKDEDLHKFFRAGPAGIRTTKAFS
                     QDCRWDTVDDDRVNGCIRNVENAVTKEGGLAVLFGNIAEDGCIVKTAGVDESIWKFTG
                     TAIVFESQEDAVAGILGGKVKEGHIVIIRYEGPKGGPGMQEMLYPTSYLKSMGLGKKC
                     ALLTDGRFSGGTSGLSIGHASPEAASGGAIALINDGDTVAIDIPNRSINLMVSDEELA
                     ARRAEMEARGDKAWQPLKREREVSFALKVFGHFATSADKGAVRDKSLLK"
     gene            complement(6556..6906)
                     /locus_tag="PXA33_001072"
     CDS             complement(6556..6906)
                     /locus_tag="PXA33_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HDL5120413.1"
                     /translation="MWHFYLMTIELNNIQYPTDASGYLTNLNDWSVELAEKIAEKEQI
                     SLTAEHWEIIYLVRDFYQEYKTSPAIRMLVKTMAQKFGEEKGNSRYLQRLFPDGPAKQ
                     ATKIAGLPKPAKCL"
     gene            complement(6935..7600)
                     /locus_tag="PXA33_001073"
     CDS             complement(6935..7600)
                     /locus_tag="PXA33_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BAX inhibitor protein"
                     /protein_id="HDL5120414.1"
                     /translation="MENRLAHSYSTGESLISTHKVLRNTYMLLAMTLTFSAVVAFVAI
                     QMNAPAMPWWGLLVGFYGLLFLTNATSNSSAGILSVFALTGFLGYTLGPILNRYIGAG
                     LGDIVALAFGATALVFFACSAYVLTTKKDMSFISGMMVALFVVLLVGIIANIFLAIPA
                     LSLALSSLFVIFSSGAILLATSNIIHGGETNYIRATVDLYVSIYNLFVSFLQIFGVLG
                     SDD"
     gene            7806..8354
                     /gene="pgsA"
                     /locus_tag="PXA33_001074"
     CDS             7806..8354
                     /gene="pgsA"
                     /locus_tag="PXA33_001074"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HDL5120415.1"
                     /translation="MKLNFPTYLTLFRVGLTPLFIVAFYLPTKYAPEISTLIFFIASI
                     TDAFDGYLARKWNQTTRLGAFLDPVADKVLVAVAFVCVAEYYHTWWITIPICIMIARE
                     IIISALREWMAELGERASVAVSIWGKVKTTAQMLALGGMLWRQNQLMEILAFILLYIA
                     VGLTIWSMMQYLNASKNSLLKS"
     gene            8432..10270
                     /gene="uvrC"
                     /locus_tag="PXA33_001075"
     CDS             8432..10270
                     /gene="uvrC"
                     /locus_tag="PXA33_001075"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HDL5120416.1"
                     /translation="MSEFNAKSFLLNVPHQPGVYRMYDSKGTIIYVGKAKDLKKRLAS
                     YFRSQLSSKKTEALVANLANIETTITHSETEALLLEHNYIKENQPKYNVLLRDDKSYP
                     YILLTKHKHPRLTAFRGSKKIAGEYFGPYPNVTAVRETLNLLQKIFPIRQCEDTYYKN
                     RSRPCLQYQIGRCLAPCVEGCYSPVEYDQQVNLVRLFLQGKDHQVVEHLVQKMEDAAE
                     ALDFEGAARFRDQIQSVRAVQEKQFVSNDRLDDLDIISIVYQNGIACVHILFVRQGKV
                     LGNRSYFPKVPSNTDLTELADTFVGQFYLQMNQHRTIPNQIVIDHQLSESKALSEILS
                     EQAGHKVSILDNAIRGDKQRYLALARTNAMAALVLQLKQESHIQSRYQALKEMLKMSE
                     IKRMECFDISHTMGNQTVASCVVFDENGPLKSDYRRFNIEGITGGDDYAAMEQALLKR
                     YNRELPPEKIPDIIFIDGGKGQLNRALDVFAKLDVEWDKSKPLLIGVAKGVERKAGLE
                     TLIISKWDKEIHLPPDSLALHLIQHIRDESHQHAISGHRKKRQKAFTESGLESIAGVG
                     AKRRQALLKYLGGMQGVKSATLEEIQSVPGISKALAEVIFDTLKNS"
     gene            complement(10275..10550)
                     /locus_tag="PXA33_001076"
     CDS             complement(10275..10550)
                     /locus_tag="PXA33_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5120417.1"
                     /translation="MKKLILLGLSVALTACSTPQQQSTVPMDMQAVQDYHQRVINKQT
                     VDKNTKQHDEPLNQSDKRSESINLPIHPIIYPSIGVGYHYGWKRYRY"
     gene            complement(10675..11028)
                     /gene="rplT"
                     /locus_tag="PXA33_001077"
     CDS             complement(10675..11028)
                     /gene="rplT"
                     /locus_tag="PXA33_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009635878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HDL5120418.1"
                     /translation="MARVKRGVIARARHKKVLKAAKGYYGARSRVYRVAFQAVVKAAQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGLSYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFAALVEKAKSAL"
     gene            complement(11137..11334)
                     /gene="rpmI"
                     /locus_tag="PXA33_001078"
     CDS             complement(11137..11334)
                     /gene="rpmI"
                     /locus_tag="PXA33_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HDL5120419.1"
                     /translation="MPKIKTVRGAAKRFKKTASGGFKRKQSHLRHILTKKTTKRKRQL
                     RHKSMVAKADQVLVVACLPYA"
     gene            complement(11598..12140)
                     /gene="infC"
                     /locus_tag="PXA33_001079"
     CDS             complement(11598..12140)
                     /gene="infC"
                     /locus_tag="PXA33_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015871208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HDL5120420.1"
                     /translation="MKPVKRVQSNRAHRLNDEIRGVKEVRLTDENGEQVGIVSLQDAL
                     NRAEEAELDLVEISPNAEPPVCRIMNYGKFIYEKEKAAKEQKKKQKVVQVKEIKFRPG
                     TDDGDYQVKLRSIIRFLEDGDKVKITVRFRGREMAHQEIGMDVLERVKADTAEIAMVE
                     SAPGKLEGRQVVMVIAPKKK"
     gene            complement(12299..14098)
                     /gene="recQ"
                     /locus_tag="PXA33_001080"
     CDS             complement(12299..14098)
                     /gene="recQ"
                     /locus_tag="PXA33_001080"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HDL5120421.1"
                     /translation="MNTTAISVLNNIFGYQKFRDGQEEVINAVLENRDCLVIMTTGGG
                     KSLCYQVPALCLDGITLVISPLISLMKDQVDQLITNGIEAAYLNSTQTLEEQQLVEQK
                     ALSGQLKLLYLSPEKVMTQGFFHFISLCKISFIAVDEAHCVSQWGHDFRPEYTLLGGL
                     RNTFPNVPLMALTATADLTTRSDILHHLRLNSPHIYLGSFDRPNIRYTVQEKFKPLEQ
                     LIKFISKQQGKSGIVYCNSRKKVEEITEKLSARKISVMGYHAGMSFEQRERVQNAFQR
                     DDIQIVVATIAFGMGINKSNVRFVVHFDLPRSIESYYQETGRAGRDDLPSEAVLFYDP
                     SDYAWLQKILLEEPESEQRNIKQHKLQAIGDFAESQTCRRLVLLNYFGEHRQEQCKNC
                     DICLDPPQQYDGLLDAQKVMSVIYRTGQIFGQHHIIAVLRGMNNLKIREHQHDQLSVY
                     GIGKEQSQHYWQNVVRQLIHLGLIIQNIANHSALQLTESARPFLRGEKQLQLALPRLG
                     ISATAFVQKQASHRYDKDLFARLRFLRKQIADLENIPPYIVFNDATLQEMAEFLPTSK
                     VEMLEINGVGERKFERFGQAFMSLIKEYQNNRK"
     gene            complement(14207..14512)
                     /gene="cyaY"
                     /locus_tag="PXA33_001081"
     CDS             complement(14207..14512)
                     /gene="cyaY"
                     /locus_tag="PXA33_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005612534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HDL5120422.1"
                     /translation="MNTAQFHQAVEQVWQQIEEKIYDEGLSVDTEIQGAVCTLTFDDE
                     SQVIINKQEAMLELWLASKLGGFHFKNVEGEWVTDEGRSFWQHLEEAFARHGESVSF"
BASE COUNT         4189 a         2834 c         3208 g         4314 t
ORIGIN      
        1 taaagtttat cagataaacg caataataaa tgcgtttttt catattattc actttaggag
       61 gaaatcaata tgtcttcatt ccaaaattgg acagaacaca cagctcacgt taagcaatct
      121 tttggtaaat tagggcaaaa acaccctaaa atgcttgaag cttataaagc attaggtaca
      181 gcggccgcag cggaagcatt agatcacaaa actcgtgaat taattgcatt agcagtagcg
      241 attacaacac gttgtgaaag ttgcatcagt gtacacgcag aagaagcggc aaaagcaggt
      301 gcaaccgaaa gcgaaattgc cggtgcatta gcgactgcga ttgcattaaa tgccggtgcg
      361 gcatatacct atgctttacg tgcattagaa gcggtggaag agcaaaaata aggagcaata
      421 aatgaagaaa ttggcttgga ttttaggctt tgcagctatg atgccaatga tgtctagtgc
      481 taaacaagca tcagttcagc aagtgcaggc tgaaaaagca gcgggcattt ggattgatgt
      541 acgttctgct gaagaatttc agcaagggca tttaaatggt gcaattaatg taacgcatac
      601 tgaaattgca caacgtatcg cagaagttgc tccagataaa aatcagccga ttcatcttta
      661 ttgccgctcg ggtcatcgag ctgaagcggc gcttaatgag ctaaaaaaat taggttacag
      721 caatgtaact aatcatggtg gttatgacga tctgattaaa gccgggttga aataactgtt
      781 ttgagtgcca agcatttgct tggcactttt cgtttatatc tgaggttaaa aattatgagt
      841 gcttttaaca attttgttaa ttttgttgca aatattgttg ctccaatgca acgccaattc
      901 attaactttg tacgtattgc catttgtatc gtgatggttt ggattggtgg tttaaaagtc
      961 tgccaatatg aagcagacgg tattgcccat tttgtgtcta atagcccgtt ctttagttat
     1021 atgtacaaaa aaggcccgaa tttagttgat gacggcacag gtaaaatggt gatggaatat
     1081 acccttcaca aaaaccctga aggtaaaatg gtggcaaaaa acatcgaatg gcataaagaa
     1141 aacggcactt atactgcatc ttatatcatc ggtgcaacca ttgtgacggt tggtttattg
     1201 acgttatccg gtatttggtt cccggtttcc ggtatggcgg ggggcttact cacctttggt
     1261 atgtcgattg ttacactctc ttttatgatc accacccctg aaatttgggt gccgaattta
     1321 ggtggggata tgccaacccc agcgcatggt ttcccatatc tgtcagcagt cggtcgttta
     1381 attgtgaaag atgtgattat gatggcaggc ggcttggttg ccgcagcgga atgtgcaaat
     1441 cgcattttag ccagacgtaa agcgattgct taattgatag tggtaatgca agcggtcatt
     1501 ttcttgtaaa attttgcaat aaaatgaccg cttgtttttt gtcaatttgc cataatttca
     1561 agtataattc aattacgtaa tcgtttgctt ttatggagag gaataaaatg acaacaatcg
     1621 gtacaccgtt aagaacgaac gctacaaaag tgatgatgtt aggttcgggc gagttgggga
     1681 aagaagtggt aatagagctg caacgcttag gcgtagaagt gattgcggtg gatcgttatg
     1741 acaatgcacc tgcacagcaa gtggctcacc gtgcttatac gatttcgatg ttagacggga
     1801 atgcattgcg agatttggtg gagaaagaaa aaccggattt tattgtgccc gaagtagaag
     1861 ccatcgcaac tgcaaccttg gttgagttag agcaagaggg atataacgta atcccaacgg
     1921 caaaagcaac gcaattaaca atgaatcgag aaggcattcg ccgattggcg gcagaagagt
     1981 tgggcttaaa aacgtcgcct tatcgttttg tggataattt tgaacagttc cagcaagcga
     2041 ttcaagaaat cggcattccg tgtgtggtca aaccgattat gtcttcttcc ggacacgggc
     2101 aatcagtaat taaatctgaa gcggatattc aacaggcttg ggattattcg caacagggcg
     2161 gacgtgccgg cggtgggcgt gtgattgtag agggttttat caaattcgat tatgaaatta
     2221 cccagcttac cgttcgccat attcacggca cgtcgttttt agctcccatc gggcatattc
     2281 aggttgatgg cgattatcgt gaatcttggc aaccgcagca aatgtcggac attgcattga
     2341 aaaaagctca agaaaccgca gaaaaaatta cctctgcact gggcggaaga gggattttcg
     2401 gcgtggaact ttttgtctgt ggcgatgaga ttatttttaa cgaagtctcc ccacgtccac
     2461 acgataccgg aatagtaaca atggcatcac aagagctttc tcaatttgct ctgcacgccc
     2521 gtgcgatttt gggcttaccg attccggaaa tttaccgcat cagcccggca gcttctaaag
     2581 cgattgtggt tgaagggaaa tcggataatg tgcgttttgg cggtgtagat aaggtattag
     2641 ccgaaatcgg gacgaatatc cgcttattcg gtaaaggcga agtaaacgga caccgccgat
     2701 taggggtaat tcttgctcgt gatgaaaata cagtgagagc attagaaaaa gctcggcgag
     2761 cttatgataa gttagatatt caattataaa gtcaggattt tgatattcgt tgatagtagg
     2821 cattttatgc ctactatcct ataggcctct aatcaaattg gatgaaaaag ttgaagattg
     2881 ttaatcctct ttgccgagaa tttggttcac ttcgctctta tacaatactg cttttgctcc
     2941 aaatacggct tgtacaccgc caccgacttc cagcacgtca atcgcaccga gttttttcag
     3001 tgttgcttta tccacggctt tcacctcttt cactgccaca cgcagacggg taatacaagc
     3061 atccacattt tcgatattat ttgcagagcc aagagcggcg ataattttat gggcattttc
     3121 agtcagtgag ctggtttgtt gttcgactac ctcttcactc tcttccgcac gtcccggtgt
     3181 cattacgttg aattttttga tcaggaatag gaacgagaag tagtaaagtg ccgcccacgg
     3241 caagccgact atgatcactc gaagccagtt agtgttctcg ttgccttgca aaatgccaaa
     3301 cagggtgaag tcgatcacgc caccggagaa ggtgttacca atcgagatgt tgagtaagtc
     3361 tgcaatgtag aacgacacgc cgtctaaaaa agcgtggaac acatagagcc acggggcaac
     3421 aaacaggaac ataaattcaa tcggctcggt aatgccggtg ataaaagagg tgagcgctgc
     3481 acctaagaac agaccgctaa tcgcaccacg gcgggctttc ggcacagcgt gatacatcgc
     3541 taaacaggcg gcaggtaagc caaacatcat cgtatcgaaa cgtccagcga agaaacgtgt
     3601 accttcggta aataagcctt gatgggttgg atcggcgagt tgggcgaaga agattttttg
     3661 cgccccgata atggtttcac catttaccac ttctgttccg cccaattcgg tgtaccaaaa
     3721 cagcgggtag atcatatggt gtaagccgac cgcaccgctt aaccgcatta agaaaccgta
     3781 gaaaaaggtg ccgatttcgc ccatttccgc aatcccttgc cctgctgaaa ccaaccactg
     3841 ttggaaagcc ggccagatca aaaagaacac tgccccgaca aaaatcgcag caaaggcggt
     3901 cacaatcggc acaaaccgag agccgccaaa gaagccgaga atttgcggca gctgaatgtt
     3961 gtggtatttg ttatgcagtt tcaccgtaat caagcccatt accagcgagc cgatcacacc
     4021 ggtatcaatc cctttggttt caggaggaaa cagcggaatg agggcagaaa tcgtgcctgt
     4081 cataatcaaa taaccgacca ctgctgccag tgccgctacg cctttatctc gttttgctaa
     4141 tccaatgcct aaaccgatac acagtaataa tgcaaggttg ccaaacacca cactgcctgc
     4201 cgatgccata atctggaaaa tgccctgtaa agtcgggtta tccagtatcg ggtaggcttg
     4261 caccgttgcc ttattcgaca aagctccgcc aatccctaac agcaaaccgg ctgccggtaa
     4321 aatcgcaatc ggcagcataa aagcttttcc gacttgttgc agttgtttga acataaaatt
     4381 tctccttttt tatggttgta tttgatggtt tatagcatac tgaaatttag ccgtatgagg
     4441 tgtgatataa ctcacagatt tgcaaaattt ttagcaaatt cgaccgcttg taaaaaaatt
     4501 accgcttgcg gaaaaattga agtagaatta agcatttttt tagcaaacac aacataaaaa
     4561 atttggagaa gattatgcct attttacgtt cagcaacttc cactcagggt cgtaatatgg
     4621 cgggggctcg tgcattatgg cgtgcaaccg gtatgaaaga aaatgatttt ggtaagccga
     4681 ttattgcagt ggtgaactca ttcacccaat ttgtgccggg gcacgtccac ttaaaagata
     4741 tggggcagtt ggttgcggca gaaattgaaa aagccggtgg ggtggcaaaa gaattcaaca
     4801 ccattgcggt tgatgacggc attgcaatgg gacacggcgg aatgctttat tctttaccaa
     4861 gccgtgattt aattgcagac agtgtggaat atatggtgaa tgctcactgt gctgatgcga
     4921 tggtgtgtat ctctaactgc gataaaatca ccccgggaat gttaatggca gcaatgcggt
     4981 taaatattcc gactattttt gtctctggtg gcccgatgga agccggaaaa actaaacttt
     5041 ccgatcaatt aattcgcttg gatttagtcg atgcaatgat cgaaagtgcc gacccgaatg
     5101 tgtctgatga acgtattgat gcgattgaac gtagtgcttg cccgacttgt ggttcttgtt
     5161 caggtatgtt caccgcaaac tcaatgaact gtttaactga agctttaggc ttaagtttac
     5221 cgggcaatgg ttcaatgttg gcaactcatg ctgaccgtaa agagcttttc ctaagagcag
     5281 gtcgtgaaat tgtggaactt tgccgtaaat attaccaaca agacgatgag agcgtgttac
     5341 ctcgctcaat cgctaccttc gatgcatttg aaaatgcaat gagccttgat attgcaatgg
     5401 gtggttcaag taataccgtg cttcatttac tagcgactgc tcaagaagcc ggcgttgatt
     5461 ttaaaatgga agatattgac cgcctctctc gtgtcgtgcc gtgcttaagt aaaattgccc
     5521 cgaataccaa taaataccat atggaagacg tacaccgtgc cggtgggatt atgggcttgc
     5581 tgggcgaatt agatcgtgca ggtttaatcc atcgccacgt taaaacagtg ctaggtttaa
     5641 cgatagaaga gcaactgaat caatacgata ttattcgtaa taaagatgaa gatttacaca
     5701 aattcttccg tgcaggtccg gcaggaattc gtactactaa ggctttctcg caagattgcc
     5761 gttgggacac tgttgatgat gaccgtgtaa acggctgtat tcgcaatgtg gaaaatgctg
     5821 tgaccaaaga gggcgggtta gcggtattat ttgggaatat tgcagaagat ggatgtattg
     5881 ttaaaaccgc aggggtagat gagtctattt ggaaattcac tggcaccgca atcgtgtttg
     5941 aaagccaaga agatgcggta gcaggtattt taggcggaaa agtgaaagaa gggcatattg
     6001 tgattatccg ctatgaaggt ccgaaaggcg gcccgggtat gcaagaaatg ctttatccaa
     6061 ctagttattt gaaatcaatg ggcttaggca aaaaatgtgc tttattaact gacggtcgtt
     6121 tctctggcgg tacctcaggg ttatctatcg gacacgcttc accggaagcc gcttcaggag
     6181 gagcgattgc cttaattaat gatggcgata cggtggcgat tgatattccg aatcgttcga
     6241 ttaacttaat ggtttcagat gaagaacttg ctgctcgccg tgcagaaatg gaagcccgtg
     6301 gcgataaagc gtggcagccg ctaaaacgtg agcgagaagt gtcgtttgca ctaaaagtgt
     6361 ttggacattt tgccacctct gctgataaag gtgcggtgag ggataaatcg ctgttaaagt
     6421 aaataaatta gaaaaaagaa gcatttcaat aatatattga tctgctcttt attcagctga
     6481 atatttaatc tagctgaatg gagagcagat cgatagccta atttacatct ttttgttttg
     6541 atacagggaa atgtattaca agcattttgc cggttttggc agacctgcaa ttttagtggc
     6601 ttgttttgca ggaccatcag gaaacaagcg ttgtaaataa cggctgttgc ctttttcttc
     6661 accgaatttt tgtgccattg tctttaccag cattcgaatt gctggtgaag ttttgtattc
     6721 ttggtagaaa tcccgcacca gataaataat ttcccaatgt tctgcggtta agctaatttg
     6781 ctctttttcg gcgatttttt ctgctaattc tacagaccaa tcatttaaat tagttaaata
     6841 gcctgaagca tcggttgggt attggatatt atttaattca atcgtcatca aataaaaatg
     6901 ccacataatg tggcattcct cttaaactaa aacgctagtc gtcactacct agcacaccga
     6961 agatttgtaa gaagcttaca aataggttgt agatagacac atataaatca acggttgcac
     7021 gaatgtagtt ggtttcgcca ccgtgaataa tgttgctggt tgccagtaaa atcgcaccgc
     7081 ttgagaaaat gacaaacaag ctgcttaggg ctaaacttaa tgccggaatt gctaagaaga
     7141 tattggcaat aataccgact aataatacca caaacagggc aaccatcatt ccgctgataa
     7201 agctcatatc ttttttggtg gttaatacat aagctgaaca agcgaagaaa accaatgcgg
     7261 ttgcaccgaa tgcaagggcg acaatatccc ctaaacccgc accgatgtaa cggtttaaaa
     7321 taggacctaa ggtatagcct aagaagccgg ttaaagcaaa tacgcttaag atacccgcac
     7381 tgctgtttga ggtggcattc gtcaagaaaa gtaagccgta aaagccgacc aataatcccc
     7441 accacggcat agccggtgcg ttcatttgaa ttgcaacaaa agcaacaact gcggagaaag
     7501 tcagggtcat tgccaataac atataggtat tgcgaagcac tttgtgggtg ctaattagag
     7561 attcaccagt tgaatagctg tgagctaaac gattttccat aaaaattcct tctgtttttg
     7621 tgtttaaatc ggaacatccg atggattaat atctatggat aaatttccat aaatcaagaa
     7681 gtaaggtagc cgtaatttat tgtaaagtca atgcgatttt gtaacagaag agtaaaaaaa
     7741 aacatttagt tgtgttacac ttaataccac tctactttat attccttttc atcgatgaat
     7801 tcgctatgaa gctcaatttc ccaacttatt taaccttatt tcgggtagga ttaaccccac
     7861 tttttattgt ggctttttat ctgccgacaa aatatgcgcc tgaaatttca acacttattt
     7921 tttttattgc atcgattacc gatgcctttg acggctattt agcacgcaaa tggaatcaaa
     7981 ccacccgact tggtgcattt ttagatcctg ttgcggataa agtcttggta gctgttgcgt
     8041 ttgtctgcgt ggcggagtat taccatactt ggtggattac tattccaatc tgcattatga
     8101 ttgcccgtga aattattatt tcggctttgc gtgaatggat ggcggagctt ggggaaaggg
     8161 ccagtgttgc cgtttcgatt tgggggaaag taaaaacgac tgctcaaatg ctcgcattag
     8221 gtggaatgtt atggagacag aaccaactaa tggagatttt agcctttatt ctgctttata
     8281 ttgcagtggg tttaacgatc tggtctatga tgcagtattt aaatgcttca aagaatagtc
     8341 tattgaagtc ataagcggtc atatttttat tcttttttgc aatttgtaga gtggaaatat
     8401 cgctctacaa ttttcaattt tatcaactct aatgtctgaa tttaacgcta aatccttcct
     8461 gttaaatgtg cctcaccaac cgggtgttta tcgtatgtat gatagtaaag gcacgataat
     8521 ctatgtgggt aaagccaaag atttaaaaaa gcgtttagcc agctattttc gtagccaact
     8581 ttccagtaaa aaaacggaag ccttggtggc aaatcttgct aatattgaaa cgacgatcac
     8641 tcattctgaa acagaggcgt tactccttga acataattat attaaggaaa atcagccgaa
     8701 atataatgtg ttgctcagag atgacaaatc ttatccttat attctgttaa ctaaacataa
     8761 gcatcctcgt ttaacagcat ttcgtggtag taaaaagatc gccggtgaat attttgggcc
     8821 ttatccgaat gttacggcag ttcgggaaac actgaattta ttgcaaaaaa tttttcctat
     8881 tcgtcagtgt gaagatactt attataaaaa ccgttcacga ccttgcttac agtatcagat
     8941 cggacgctgt ttagcccctt gtgtagaggg ctgttattca cccgttgaat acgatcagca
     9001 ggttaatttg gttcgtttgt ttttacaggg aaaagatcac caagtagtcg agcatctggt
     9061 acagaaaatg gaagatgcgg cggaggcttt ggattttgaa ggtgcggcac gttttcggga
     9121 tcaaattcaa tcagtaagag cggtgcaaga aaaacaattc gtctctaatg ataggctaga
     9181 tgatcttgat attatctcga tcgtttatca aaatgggatt gcctgtgtgc atatcttatt
     9241 tgttaggcaa ggtaaggtac taggcaatcg ttcttacttt ccaaaagtgc caagtaatac
     9301 agatttaacc gagctcgcag atacctttgt cggtcagttt tatttacaaa tgaaccagca
     9361 ccgcaccata cctaaccaaa ttgtaattga tcatcaatta agtgaatcta aggcactcag
     9421 tgaaatttta agtgagcaag cagggcataa agtcagcatt ttagataatg ccatcagagg
     9481 tgataaacag cgttatttag ctttagcgag aactaatgca atggcagcat tagttttaca
     9541 acttaagcaa gaatcccata ttcaaagccg ttatcaggca ttgaaagaaa tgttgaaaat
     9601 gtcagagatt aaaagaatgg agtgctttga catcagtcat acaatgggca accaaacagt
     9661 ggcttcttgc gtggtgtttg atgaaaatgg gccgctaaaa tcggattacc gccgttttaa
     9721 cattgagggg attaccggag gtgatgatta tgcagcaatg gagcaggctc tcttgaaacg
     9781 ctataatcga gaattaccgc cagaaaagat ccctgatatt atttttattg atggtggcaa
     9841 agggcagttg aaccgagcgt tagatgtctt cgctaaactt gatgtggaat gggataaatc
     9901 gaaaccgctt ttaattggtg tggcaaaagg cgtagaacgt aaagctgggc tggaaacttt
     9961 aattatcagc aaatgggata aagaaattca cttaccgcca gatagtcttg ccttgcattt
    10021 aattcagcat attcgagatg aatcccacca gcatgccatt agtgggcatc gtaaaaaacg
    10081 ccaaaaggcc tttaccgaaa gtggattaga atctattgcc ggtgtcggtg caaaacgccg
    10141 acaagcgttg ctaaaatatt taggcggaat gcagggggtg aagtctgcaa ccttggaaga
    10201 gattcaatca gttccgggaa tttctaaagc gttggcagag gttatttttg atacattaaa
    10261 aaatagttaa attatcaata gcgatatcgc ttccagccgt aatggtagcc cactcctatg
    10321 cttgggtaaa ttatagggtg aattggcaga tttatgcttt ctgagcgttt atcgctttgg
    10381 tttaatggct catcatgttg ctttgtattt ttatcaacag tttgcttatt gattactcgt
    10441 tgatggtaat cctgaactgc ttgcatatcc attggtactg tgctttgttg ttggggtgtc
    10501 gaacaggcgg ttagtgcaac gctcaatccg agtaaaatta attttttcat atcatttcct
    10561 tatattgcaa aatattttgc ttttttgacc gcttattttg ctattaattc ttaaaaagat
    10621 tattttatcc ataaaaaagg acgcactagg cgtcctcatt tctattcttt aaaattaaag
    10681 agcagatttt gctttttcaa ctaaagctgc gaaagcaact ttgtcaaata ctgcgatgtc
    10741 agctaagatc ttacggtcga tttcaacaga tgcttttttc aagccgttga tgaatttgct
    10801 gtaagataaa ccgttttgac gagctgctgc gttaatacga gcaatccata attgacggaa
    10861 ttgacgttta cgttgacggc ggtcacggta agcatattga gcagctttaa ctactgcttg
    10921 gaaagcaacg cgatacacgc gtgaacgtgc accataataa cctttagcag ccttaagaac
    10981 tttcttatgg cgtgctcttg caataacacc acgttttaca cgagccatta tttaatctcc
    11041 taattgtcta ttttgattgg tggtcgtcat cacgaccttt actaagaatt ctatccgtac
    11101 gtccgtactt tactacacta cgctaaccta aaaggcttat gcgtatggta agcacgctac
    11161 tactaaaact tggtcagctt tcgctaccat tgatttgtga cgtaattgac gtttacgttt
    11221 agtggttttc ttagtcaaaa tgtgacgtaa gtgagattgt ttacgtttaa aaccgccgga
    11281 agctgttttt ttgaaacgct tagcagcacc acgtactgtt ttaattttag gcattgttaa
    11341 aaaactccgc attgtttgtt agatattatg aaaataaggc gaattttgtg aacaaaatta
    11401 cttggaagca cttattctcc gctatacgat tttcatcgtt aaaaagtcaa gcaggctgct
    11461 gagtttgcct gttgagcatt ttctctttct tggctaggca aaaaagcttg cgaattttag
    11521 cgaaaatata accgctatgc aagccaattt aagctttatc aagaaaaaag cacattaaaa
    11581 caatgtgctt atcgaaatta tttcttctta ggtgcgatca ccatcacaac ttggcgacct
    11641 tccaatttgc ctggggctga ctctaccatt gcaatttctg cagtatctgc tttgacacgt
    11701 tctaaaacgt ccataccgat ttcttggtga gccatttcac gaccacggaa acgcactgtg
    11761 atttttactt tatccccatc ttctaagaaa cggataatgc tacgaagttt aacttgataa
    11821 tcgccatcat cagttcccgg acggaatttg atctctttaa cttgaacaac tttttgtttt
    11881 ttcttttgtt cttttgctgc tttctctttt tcgtagatga acttaccata gttcataata
    11941 cgacaaactg gtggttcagc atttgggctg atttccacta aatccaattc agcttcttct
    12001 gctctattta gagcatcttg aagtgataca ataccaactt gttcgccgtt ttcatcggtt
    12061 aagcgaactt cttttacgcc acgaatttcg tcattaagac gatgtgcacg atttgattga
    12121 acacgtttta caggtttaat agccttattc cttctagtaa atttttgctt aaattttacc
    12181 gcttgagagc gtataaatat tttagattgc ttgttttaag tgattaaaaa acagctaaac
    12241 gtgcggattc tataagattt tcagtgctta tgcaataata aagcccttat ttaactgcct
    12301 attttcggtt attttgatat tctttgatta aactcataaa tgcttgccca aatcgttcaa
    12361 attttcgttc accaacacca ttaatttcta acatttccac ttttgaagtc gggagaaatt
    12421 ctgccatttc ttgcaaggtg gcatcgttaa agacgatata aggtggaata ttttcgaggt
    12481 cagctatctg cttgcgtaaa aaacgtaagc gtgcgaatag gtctttgtca tagcgatgac
    12541 ttgcttgttt ttgcacaaat gcagtcgcag atatacctaa acgtggcaat gcaagctgaa
    12601 gttgcttttc tccacgcaaa aatggtctgg cactttcggt taattgcaga gcggaatggt
    12661 tagcaatatt ttgaataatc aaccctaaat gaatcagttg ccgcaccaca ttttgccaat
    12721 aatgttggct ttgttctttt ccgataccat aaacggaaag ttgatcgtgt tgatgttcac
    12781 gaattttgag attgttcatt cctcgcaata ccgcaataat atggtgctga ccgaaaattt
    12841 gaccggtgcg gtaaatcacc gacatcactt tttgggcatc aagtaagcca tcgtattgtt
    12901 gcggtggatc taaacaaata tcgcagtttt tgcattgctc ttggcgatgt tccccaaagt
    12961 agttgagtaa gactaaacgg cggcaagtct gtgattcggc gaaatcgcca attgcctgta
    13021 atttatgctg tttaatattt ctctgttcgc tttccggctc ttccaataat attttttgta
    13081 accaagcata atcgctagga tcataaaaaa gcacggcttc agaagggaga tcatctcgcc
    13141 cggctcgtcc ggtttcttga tagtaagatt cgatagagcg aggtaaatcg aaatgtacca
    13201 caaaacgtac gttggatttg ttgatcccca taccaaacgc aatagtggca accacaattt
    13261 gaatatcatc acgctgaaag gcattctgca ctcgttctct ctgttcaaaa ctcataccgg
    13321 cgtgatagcc catcacgctg attttacggg ctgagagttt ctcggtgatt tcttccactt
    13381 ttttgcgact gttgcagtaa acaataccgc ttttgccttg ctgtttgctg atgaatttaa
    13441 tgagctgttc aagcggttta aatttttcct gcacggtgta gcgaatattg gggcgatcaa
    13501 agctgcctaa atagatatgt gggctattta agcgtaaatg atgcagaata tcggagcggg
    13561 ttgttagatc tgccgtggcg gtgagtgcca tcaacggcac attcgggaaa gtgtttcgta
    13621 agccacctag cagggtatat tcagggcgga aatcgtgccc ccattgggaa acacaatggg
    13681 cttcatcaac cgcaataaag ctgattttac atagcgaaat aaagtggaaa aagccttgag
    13741 tcattacctt ttcaggggaa agatagagta attttaattg accggataag gctttttgtt
    13801 ccacaagttg ctgctcttcg agcgtttgag tggagttaag ataagctgct tcaatgccgt
    13861 tggtgataag ttgatccacc tggtctttca tcaacgaaat aagcggcgaa atcaccaaag
    13921 tgatgccgtc taggcacaaa gccggcactt ggtagcaaag ggatttgcca ccgcctgttg
    13981 tcataatcac gaggcaatca cgattttcca acacggcatt gataacttct tcttgcccgt
    14041 ctcggaattt ttgatagcca aaaatattat ttaaaacaga aattgcggtg gtgttcattg
    14101 gtagttggtt gctacttatg atagtactcg tcttctgaga gtatctttta aaatgaaaat
    14161 aaagcactca tcaaagacga gtgctgtctt agttaacaat taggcattag aaagaaacgc
    14221 tttctccgtg tcgagcaaaa gcttcttcta aatgttgcca gaacgagcga ccttcatctg
    14281 ttacccattc gccttctaca tttttaaagt ggaaaccacc caatttgctg gcaagccata
    14341 attccagcat tgcctcttgt ttattaatga taacttgcga ttcatcatca aaggttaagg
    14401 tgcagaccgc tccttggatt tcagtatcga ctgataatcc ttcgtcataa attttttctt
    14461 caatttgttg ccatacttgc tctacggctt ggtggaattg tgctgtgttc attggtgtat
    14521 ccttatttga atgttatata agtag
//
LOCUS       DANLRK010000050        14530 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.050, whole genome shotgun sequence.
ACCESSION   DANLRK010000050 DANLRK010000000
VERSION     DANLRK010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 14530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14530
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.050"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            166..597
                     /locus_tag="PXA33_001082"
     CDS             166..597
                     /locus_tag="PXA33_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3577 domain-containing protein"
                     /protein_id="HDL5120423.1"
                     /translation="MTTQTSTQKNYFNLHTTGIGYLNDIRLVEPKKGNPFYACRIAAL
                     VGESDSPEYRYFDMNVVGKETETLIKRCQKAVSEEKKVLVSFVMADLWFDTFTYSTDS
                     KFHKKGDTGVSVKGRLIRIKMIKIDGEIAYQEKPKSDEQSE"
     gene            615..1607
                     /locus_tag="PXA33_001083"
     CDS             615..1607
                     /locus_tag="PXA33_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL5120424.1"
                     /translation="MLCWALLLDVQDLRVTFKTPDGDVTAVNDLTFSLNAGETLGIVG
                     ESGSGKSQTAFALMGLLADNGRISGSAKFNGQEILNLPETQLNRLRAEQIAMIFQDPM
                     TSLNPYMKIGEQLMEVLMLHKGMDHKTAFEESVRMLDAVKMPESRKRMNMYPHEFSGG
                     MRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKSEFNTAIIMITHDLG
                     VVAGICDNVLVMYVGRTMEYGTAEQIFYTPSHPYSIGLIGAVPRVDGQEEELITIPGN
                     PPNLLRLPSGCPFQPRCVHATKQCLKAPALTLLNDQRLRACFVPAEQLIRGNAS"
     gene            1604..2593
                     /gene="oppF"
                     /locus_tag="PXA33_001084"
     CDS             1604..2593
                     /gene="oppF"
                     /locus_tag="PXA33_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000994906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HDL5120425.1"
                     /translation="MNTEKKVILEVKDLSVHFNIKDTKSLFFAKHQTLKAVNHVSFQL
                     YEGETLGVVGESGCGKSTLARAIIGLVESSGGNILWLGKELTQQSAKEWKTIRSDIQM
                     IFQDPLASLNPRMTIGSIIAEPLKIYQPHLTAEEVKEKVRAMMMKVGLLPNLINRYPH
                     EFSGGQCQRIGIARALIVEPKLIICDEPVSALDVSIQAQVVNLLKSLQREMGLSLIFI
                     AHDLAVVKHISDRVLVMYLGNAVELGDYEQVYHDTKHPYTKALMSAVPIPDPQLERNK
                     QIQLLEGDLPSPINPPSGCVFRTRCPLADQHCIADKPLLKGRDNHVVACFKAE"
     gene            complement(2697..3455)
                     /locus_tag="PXA33_001085"
                     /pseudo
     CDS             complement(2697..3455)
                     /locus_tag="PXA33_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326384.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
     gene            complement(3585..4226)
                     /gene="bioD"
                     /locus_tag="PXA33_001086"
     CDS             complement(3585..4226)
                     /gene="bioD"
                     /locus_tag="PXA33_001086"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005724996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HDL5120426.1"
                     /translation="MAKVFFITGIDTDIGKTIATGWYAKKLMQQGASVITQKMIQTGC
                     RGIAEDLLIHRKIQGIELTEEDKQGITCPYVFDYPCSPHLAAKLEPRTIERKKIETCT
                     ALLCEKYDYVLLEGAGGLCVPYNEEETTLDYLCQHQYPVILVTSGKLGSINHTLLSLQ
                     VLNSKRVSVHAVIYNLYPETDQVISQETQHFLRRYLEKYSPNTLFEIMDLISV"
     gene            complement(4244..4999)
                     /gene="bioC"
                     /locus_tag="PXA33_001087"
     CDS             complement(4244..4999)
                     /gene="bioC"
                     /locus_tag="PXA33_001087"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005755527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HDL5120427.1"
                     /translation="MDRIDKGRIKQRFSAAVSNYDQQARAQQQIHQRLLSLLSYIDRQ
                     AFQRALEIGCGTGGLTKQLMSQLQVEHWELNDLCDMREHLQSVLPQPFHFHCGDAEQL
                     PFPQQYDLIASASVVQWFEDKAGFIARCKVRLKKHGFLLFSTFTPENLTQIKHLTGVG
                     LTYPRLEDWQKWLVPEFEILALEHEEIVLHFDDPLAVLKHLKQTGVTATQQQRWTKGQ
                     LHAFCQQYQQHYRNAQHKVELTYTPMYVLARLT"
     gene            complement(5003..5650)
                     /locus_tag="PXA33_001088"
     CDS             complement(5003..5650)
                     /locus_tag="PXA33_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005758126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF452 family protein"
                     /protein_id="HDL5120428.1"
                     /translation="MKTQHISQQGDNLIVYFAGWGTPPSVVEHLILPENYDLCLCYDY
                     RDLHCDVDFSAYQHIRVVAWSMGVWVAERVMQGYPLLSATAINGTGLPCDDQYGIPEA
                     IFAGTLSGLNEDNRTKFERRICGSKTLLRDYQHYSMRPTLQEIHAELTALYTFLQQDH
                     RTDLISWSKAIVGSKDKIFPAQNQRAYWQDRCAITEIAGEHLLFPHFTHWETCWL"
     gene            complement(5647..6810)
                     /gene="bioF"
                     /locus_tag="PXA33_001089"
     CDS             complement(5647..6810)
                     /gene="bioF"
                     /locus_tag="PXA33_001089"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005719374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HDL5120429.1"
                     /translation="MIRYYQQQLADLKAQNQFRQLPQLIHRGRFIQREDNTMLNMSSN
                     DYLGLANNEALRQAFFTQYQDQLPALTSSSSRLLTGSFPIYDELESLMAQAFGRETAL
                     LFNSGYHANIGILPALADKKTLIVADKLVHASMIDGIRLAQCEFVRFRHHDYVHLEQI
                     LQKNDRTFERIIVVTESVFSMDGDCADLTQLVALKQRYPQVMLYVDEAHAIGVLGEKG
                     LGLAEQQGCINQIDILVGTFGKALGSMGAYVICDQVIRDYLVNKMRPLIFSTALPPFN
                     VAWTHFVFQQLPHLQAERAHLAQLSQHLRQAIVDIFQVPMPSESCIVPYILGDNELTV
                     RTAQRLQQQGYYCLPIRPPTVPKGTSRIRFSLTADMQVAEVEQFIACLRELAE"
     gene            complement(6807..8108)
                     /locus_tag="PXA33_001090"
     CDS             complement(6807..8108)
                     /locus_tag="PXA33_001090"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016533607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HDL5120430.1"
                     /translation="MNEQQLLECDRQHIWHPYAALNADFPLFAVERAEGVYIQLKNGQ
                     QLIDGMSSWWSAIHGYNHPRLNHAATQQLSQMSHVMFGGLTHQPAVELAQQLVNILPE
                     SLDKIFFADSGSVAVEVAMKMAVQYQHAKGETQRYKFATIRSGYHGDTWHAMSVCDPV
                     TGMHSLFAKGLPVQYFLPQPPVKFGQAWQPEAMQPLQDLLAQHGDEIAGLILEPVVQG
                     AGGMYFYSPEYLVQAKALCEPYGILLIFDEIATGFGRTGKLFACEHANVVPDVMCIGK
                     ALTGGYLTLSATITNQAVADVICSGEAKCFMHGPTFMANPLACAVAAESIKLLLASPW
                     QQRIQQIEQQLRQELRQAEALDCVAEMRVLGAIGVIEMKAPVNMASLPPRFVQNGVWV
                     RPFGKLVYLMPPYIISSDQLSQLIHGTIRTLQEEYAHKESL"
     gene            8250..9137
                     /gene="psd"
                     /locus_tag="PXA33_001091"
     CDS             8250..9137
                     /gene="psd"
                     /locus_tag="PXA33_001091"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005755522.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HDL5120431.1"
                     /translation="MNILEKKAQLSYWQRIKIAFQYVMPQLYLTRLAGWFAKQQWGAV
                     THFVIKLFAKKYHVDMSEAAKPNFSDYASFNEFFIRPLADNARPINQNPTALCLPADG
                     RISQLGHIEQDLLLQAKGHYFSLNDLLAGDEALAHHFKDGEFATTYLSPRDYHRVHMP
                     CDATLCKMIYVPGDLFSVNPFLAEHVPNLFARNERVICVFDTEFGKMVQILVGATITA
                     SMSTVWAGVINPPRPEKITTWTYEGESAVKLLKGQEMGAFQLGSTVINLFEKDRVQLA
                     SHLQVDSPVRMGEILAHQK"
     gene            9162..9716
                     /locus_tag="PXA33_001092"
     CDS             9162..9716
                     /locus_tag="PXA33_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDL5120432.1"
                     /translation="MSQQFFDQVSLESQLEVSAEAVAKGIFDVLNQNPPALELNEVSH
                     ALQTLQQQAKFKEIEAVGAQFLAENAQKAGVNTTESGLQYEVLVAGEGQIPAREDKVR
                     VHYTGTLIDGTVFDSSVKRGQPAEFPVNGVIAGWIEALSMMPVGSKWRLTIPHNLAYG
                     ERGAGASIPPFSTLVFEVELLAIL"
     gene            complement(9776..10099)
                     /locus_tag="PXA33_001093"
     CDS             complement(9776..10099)
                     /locus_tag="PXA33_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005755520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5120433.1"
                     /translation="MNWKAVLIFSVLGSLVSCASYSEHAVQRIEAGKSFAVTGNTKRI
                     NTMACQDNDDWYLDGYRVGKSFREHQQKMLIQRTAYCEEQTGKAVPDKFRHSWNSGYQ
                     QGLKR"
     gene            complement(10123..11226)
                     /gene="proB"
                     /locus_tag="PXA33_001094"
     CDS             complement(10123..11226)
                     /gene="proB"
                     /locus_tag="PXA33_001094"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005719361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HDL5120434.1"
                     /translation="MKIDKTLVVKFGTSTLTQGSAKLNLPHMIDIVRQLAQLHQAGFR
                     LVIVTSGAIAAGRHYLNHPQLPPTIASKQLLAAVGQSQLIQTWEKLFAIYDIHIGQIL
                     LTRADIEDRERFLNARDTLRALLDNQIIPVINENDAVATSEIKVGDNDNLSALVAILV
                     QAEQLYLLTDQQGLFDRDPRKHPDAKLIADVDKITDHIRAIAGGSGTSLGTGGMSTKI
                     SAADVATRSGIETIIAPGNRPNVIVDLAYGQAIGTKFSVQADRLESRKQWLYAAPSAG
                     IITIDAGAENAMLIQHKSLLPAGIVNVEGRFSRGEVVKIRTQQGKDVALGMPRYNSDA
                     LHLIQGKHSQDIEQVLGYEYGAVAVHRDDMIVL"
     gene            11392..12380
                     /locus_tag="PXA33_001095"
                     /pseudo
     CDS             11392..12380
                     /locus_tag="PXA33_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252470.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
     gene            12455..13102
                     /locus_tag="PXA33_001096"
     CDS             12455..13102
                     /locus_tag="PXA33_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HDL5120435.1"
                     /translation="MTINTYAKFAPTVFVAKCSESHTKGEIITLTSKYGNETEVQVHN
                     LVKQDGEHYFYSFTRCDGMDSQSFAQQKAERYQGYADNAAKRSQDWVNAANEGCDFLS
                     LGEPIKIGHHSEKRHRNLIERNARRMDNAVAEMNKAASYESRIAYWEKMAGKIDLSMP
                     ESLEYFKFELDKAKTKHKELKDNPEKREHGYSLTYAKKAVNELEKKVKLAELLWA"
     gene            13126..13533
                     /locus_tag="PXA33_001097"
     CDS             13126..13533
                     /locus_tag="PXA33_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme utilization protein"
                     /protein_id="HDL5120436.1"
                     /translation="MNRQGIYINGKQVFDSQGEDIQSIGFFGCNIQTVVGINKGSILV
                     SSNSNQLLVNGQSIELGSEPVLKIEVLGNVQKLSAGAADVSITGDAGSVDTVSGDVSI
                     KGNVASYVKTVSGDVRCRAITGSVETVSGDIYR"
     gene            13631..13981
                     /locus_tag="PXA33_001098"
     CDS             13631..13981
                     /locus_tag="PXA33_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5120437.1"
                     /translation="MTTINLSQQERELVEFVWNNRNTPWFFHCSVSNVSRNNTSRQIA
                     VCVIKDNQLISLDELIAKLCGYKLRQNGLYVTVHNMDAVFDTLYRFMCKLNDEMEANE
                     NVCPYLSLAHYEVF"
     gene            14072..14434
                     /locus_tag="PXA33_001099"
     CDS             14072..14434
                     /locus_tag="PXA33_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5120438.1"
                     /translation="MIDTFFDLDDTSCYLGNWEDILKEYDDCLSVEDFWHSAKEFDTV
                     PHFGNLYQERVILKLIARFCIELNIEQDSGLVEFDYYINAIDTHFYINSQRICDIDDW
                     NEMLDKIREEMTPAKLVA"
BASE COUNT         4195 a         3159 c         3006 g         4170 t
ORIGIN      
        1 agcattctga aagggaacgc cacttttgtg ggaatgccac ctaaaccctc taccatctca
       61 gtgaagcatc attgagattt cattttactt ccacctcgcg gggaacattt ttcccgtgtt
      121 gggcaaggat gtttctccgc ttttttacat ttggagaaac aaactatgac aactcaaact
      181 tctactcaaa aaaattactt caaccttcat acaacaggca ttggctatct aaacgatatt
      241 cgtttggtag agcctaaaaa aggaaatcct ttttatgcct gccgtatagc agctctggtt
      301 ggcgaatctg actctcctga gtacagatat tttgatatga atgtagtcgg taaagagact
      361 gaaacactta tcaaaagatg ccaaaaagca gttagtgaag agaaaaaagt gcttgtgtca
      421 tttgtaatgg ctgatttgtg gttcgataca tttacttact caacagatag taaattccac
      481 aaaaaaggcg ataccggtgt atctgtaaaa ggtcgcttaa tccgcatcaa aatgattaaa
      541 attgatgggg aaatagccta ccaagaaaag ccaaaatcag atgagcaatc tgagtaattt
      601 tacctaagcc cgacatttta tgttgggctt tattactcga tgtacaagat ttgcgcgtaa
      661 cttttaagac cccagatggc gatgttactg ccgtcaatga tctgactttt tctttaaatg
      721 ccggtgaaac cctcggcatt gtcggcgaat ctggctcagg aaaatcgcaa accgcttttg
      781 ctctaatggg gctattagca gataacgggc gaatttcagg atcagcaaaa tttaatggac
      841 aagaaattct caacttgcct gaaacacaac ttaaccgctt gcgtgctgaa caaattgcga
      901 tgatcttcca agatccaatg acctcgctga acccttatat gaaaatcggc gaacagctaa
      961 tggaagtact gatgttgcat aaaggcatgg atcataaaac tgcgtttgaa gaatcggtac
     1021 ggatgttaga tgcggtgaaa atgccggagt cacgtaaacg tatgaatatg tatccgcatg
     1081 aattttccgg cggtatgcgt cagcgtgtga tgattgcgat ggcattactt tgccgtccga
     1141 aactcctcat tgcggatgaa cccacaactg cgctcgatgt gacggtgcaa gctcaaatca
     1201 tgaccttatt aaacgaatta aaaagcgagt ttaataccgc cattatcatg attactcacg
     1261 atctcggtgt ggtcgcagga atttgcgata atgtgttggt gatgtatgta ggcagaacca
     1321 tggaatacgg caccgctgag caaattttct ataccccaag ccacccttat tctatcggct
     1381 taattggtgc agtgcctcgc gttgacggac aggaagaaga actcattact attccgggta
     1441 atccacctaa cttattacgc ttaccgagtg gctgtccttt ccaaccacgt tgcgttcatg
     1501 caactaaaca gtgcctcaaa gcaccggcgt taaccttgtt aaatgatcaa cgtttacgcg
     1561 cttgttttgt ccctgccgag caattaatcc gaggtaacgc atcatgaaca cagagaaaaa
     1621 agtgatccta gaagtgaagg atttaagcgt tcatttcaac atcaaagata cgaaatcttt
     1681 attctttgct aaacaccaaa ctttaaaggc cgtcaatcat gtctcttttc agttatacga
     1741 aggcgaaacc ttaggtgtgg tcggtgaatc gggctgtgga aaatcaactc tcgcacgcgc
     1801 gattattggc ttagtggaaa gctctggcgg taatatttta tggctaggta aggagttaac
     1861 tcaacaaagt gcgaaagaat ggaaaacgat ccgtagcgat attcaaatga ttttccaaga
     1921 tcctttagcc tccctgaatc cacggatgac cattggcagt atcattgccg aaccattaaa
     1981 aatttaccaa ccccatttaa ccgcggaaga agtgaaagaa aaagtacgcg cgatgatgat
     2041 gaaagtgggc ttattaccta acttaatcaa tcgctatcca catgaatttt cgggtggaca
     2101 atgtcagcgt atcgggattg cgcgcgcctt gattgtggaa ccaaaactca ttatttgcga
     2161 tgagccagtg tccgccttag atgtttcaat ccaagcacaa gtagtgaatt tattgaaatc
     2221 tttacaacgc gaaatggggc tatcattgat tttcattgca cacgaccttg ctgtggtgaa
     2281 acacatttcc gatcgtgttt tggtcatgta tcttggtaac gcggtggaat taggtgacta
     2341 tgaacaagtc tatcacgata ctaagcatcc ttataccaaa gctttgatgt ctgcggtacc
     2401 gattcctgat ccgcaattag aacgcaataa acaaattcaa ttgcttgaag gcgatctccc
     2461 ttccccgatt aatccgccgt cgggctgtgt gttccgcacc cgttgcccac tggcagatca
     2521 acactgtatt gcagacaaac cgctcctcaa aggacgagat aatcacgttg ttgcttgttt
     2581 taaagccgag taatacacac aaccgcacaa aaaagtgcgg ttgttttttt gcgaatattc
     2641 acagtcagac tttcgtgata ctttcgctct gagataagga ttacgataca gagaagttat
     2701 tcaacaatca cacgtgcatt gatcggttgc actggacgat tattgtgtcc tttcatcaag
     2761 gtcgcaaacg cctcaacttg agattggcta atgttgtcat agtgttttaa cactaaccaa
     2821 tttacacctt cagaacaagg tggtgtagtt aatgacccgc tgaaacgata gtaatcgcgt
     2881 tttttcggaa ttaatgttgc tgggtcaaaa gcggtactaa gcgtatattt ctcccctgct
     2941 ttttgcggga agttttgcca aattggggct aaccattggt tttcacttga taatacatag
     3001 agcacgccaa gcacagcaag attcccttca ttgtctgcgt gtacaaaatg tgcttccatc
     3061 gggaaggatt tacctcaaat taagttttca ctcggcacat ggaaatggaa ttgtttaagg
     3121 gtaaaagtct ttccattaag ctgcatgcga ttacttgcct ctttgaaatc cacttgaata
     3181 gtatgcccat tattgacaat ctccacattg gaaggtcgat aatccagctt aatcggcgct
     3241 aacttaccat caatcgtatg cttgatatct attggtgttt ggtttttacc ttcgccacaa
     3301 atacggaatt tgggtgaaag ttcagcccaa tgttctggcg attcgtgtcc ggtgtatccc
     3361 caatgcgtgt gcgtttcttc tttcttaagg gggcttgtac acgctgtcaa tgctaatgca
     3421 cctaacgtaa gcacaaaaag gtttgatttt ttcatagcct agctcctgat taaaagagat
     3481 gaattgatga gacaacctaa actcaatgat gccttaatcc gagacgcttt catcttaggc
     3541 aaaacaacac tccatttcaa gtataacttg ctcaagaata tatcttagac cgaaatcaaa
     3601 tccattattt caaataaggt attaggcgag tatttctcta aataacgacg taaaaaatgt
     3661 tgtgtttctt ggctaatgac ttgatctgtt tccggataca agttgtatat cacagcatgc
     3721 acacttactc ttttggagtt gagtacttgt aaactcaata aggtatgatt aatactacct
     3781 agcttgccag aggtaactaa aatcacgggg tattgatgct gacaaagata atcaagggtg
     3841 gtttcttcct cattataagg tacacacaag ccgcccgccc cttccaataa tacataatca
     3901 tatttttcac acaaaagtgc ggtgcaggtt tcaatttttt tacgttcgat cgtgcgcggt
     3961 tctaatttcg cggctaaatg cggtgagcaa ggataatcaa acacgtaagg acaagtgata
     4021 ccttgcttat cttcttctgt aagctcaata ccttgtattt ttcggtgtat gagtaaatct
     4081 tcggcaattc ctcggcagcc tgtttggatc atcttttgcg tgatgacact tgcgccttgt
     4141 tgcatcagtt tcttggcgta ccaacctgtg gcgatggttt tgccaatatc ggtgtcgatg
     4201 ccagtgataa aaaaaacctt tgccatactt tttccttcta agattaagtt aaacgcgcca
     4261 gcacatacat cggcgtgtaa gtgagttcta ctttgtgttg tgcgttacgg taatgttgtt
     4321 gatattgttg gcaaaaggcg tgcaattgcc cttttgtcca cctttgctgc tgtgtggcag
     4381 taacgcctgt ttgtttcaaa tgttttaaca ccgctaaggg atcatcaaag tgcaacacaa
     4441 tctcctcgtg ttcgagggct aaaatctcaa attcaggcac tagccacttt tgccagtcct
     4501 caagacgtgg ataagttaac cccacaccgg tgaggtgttt gatctgtgtt aaattctcgg
     4561 gcgtgaaagt actgaataac aaaaatccgt gcttttttaa gcgcacttta caacgcgcaa
     4621 taaaacccgc tttatcttca aaccattgca ccacggaagc actggcaatc aaatcatact
     4681 gctgcggaaa gggaagttgt tccgcatcgc cacaatggaa gtgaaaaggt tggggcaaca
     4741 ctgattgcag atgctctcgc atatcgcaaa ggtcattcag ctcccagtgt tcaacctgca
     4801 actgtgacat taattgcttc gttaatccac cagtaccaca tccaatctct aatgcacgtt
     4861 ggaatgcttg tctgtcgata tacgacaata aactaagcaa acgttggtga atctgctgtt
     4921 gtgctctcgc ttgctgatca tagttcgata cagcggcaga aaaacgctgt ttaatgcgtc
     4981 ctttatcaat acgatccatg tgctacaacc aacaagtttc ccaatgggtg aagtgaggaa
     5041 acaacaaatg ctcccctgcg atttctgtta ttgcacaacg atcctgccaa taagcccgct
     5101 gattttgtgc agggaaaatt ttatcttttg agccgacaat cgccttgctc cacgagatta
     5161 aatctgtacg gtgatcttgt tgtaaaaagg tataaagtgc ggtcagttca gcatgaattt
     5221 cctgtaatgt gggacgcatg gaatagtgct gataatctct taacagcgtt tttgaaccac
     5281 aaatacgacg ttcaaattta gtccgattat cttcatttaa accagataac gtgccagcaa
     5341 aaatggcttc gggaatacca tattggtcat cacaaggtaa gcccgtgcca ttgatcgccg
     5401 tagcagatag cagtggataa ccttgcatca cacgctccgc tacccacacg cccattgacc
     5461 atgccaccac gcgaatatgt tgataagcgg aaaaatcaac atcgcaatgc agatctcgat
     5521 aatcatagca aaggcataaa tcataatttt ccggcagaat caggtgctcg accacgctcg
     5581 gcggtgtgcc ccaccctgca aaatagacga tcagattgtc gccttgctgt gaaatatgtt
     5641 gtgttttcat tcagcaagct cccgtaaaca agcaataaat tgttccactt ccgccacttg
     5701 catatctgca gtcagtgaaa aacgaatacg agaggttcct tttgggactg tcggcgggcg
     5761 aatcggtaag caataatagc cttgttgttg caaacgttgc gccgttctta ctgtcagttc
     5821 gttatcgccc aaaatataag gtacgataca actttcactt ggcatcggga cttgaaaaat
     5881 gtcaacaatc gcttggcgta aatgttgact aagctgagca agatgtgcgc gttcggcttg
     5941 caaatgtggt aattgttgaa agacaaaatg cgtccatgcc acattaaacg gcggaagtgc
     6001 cgtggaaaaa atcaatgggc gcattttatt aaccaaataa tctcggatca cttgatcgca
     6061 aatcacataa gcccccatag agcccaacgc cttgccaaac gtaccgacta aaatatcaat
     6121 ttggttgata cagccttgtt gttctgctaa ccctagcccc ttctccccca atacgcctat
     6181 cgcatgggct tcatctacat ataacatcac ttgcggatag cgctgtttta atgcgaccaa
     6241 ctgggtgagg tctgcacagt caccatccat actaaacacg gattccgtga ccacgataat
     6301 ccgctcaaaa gtgcgatcat ttttttgcaa aatttgttca aggtggacgt aatcatgatg
     6361 acgaaaacgc acaaactcac attgtgccaa gcgaatgcca tcaatcatac tggcatgtac
     6421 cagtttatcc gccacaatca gcgttttttt gtccgctaaa gcaggcaaaa tcccaatatt
     6481 agcatgataa ccgctattaa atagcaaggc agtttctcgt ccaaatgcct gcgccattaa
     6541 gctttctagc tcatcataaa tcggaaaaga gcctgttaat aaacgggagg aagaagaagt
     6601 caatgcaggg agttgatctt ggtattgcgt gaaaaatgcc tgacgcaagg cttcgttatt
     6661 ggctaaacct aaataatcat tggaggacat attcagcatc gtattgtcct cacgttgaat
     6721 aaaacgccct cggtgaatta gttggggcag ttggcgaaat tgattctgcg cctttaaatc
     6781 agctaactgc tgctgataat acctgatcat agagattcct tatgtgcata ttcttcttga
     6841 agggtgcgaa ttgtgccatg aattaattga gacaattgat ccgatgagat gatatacggt
     6901 ggcatcaaat acactaattt gccaaatgga cgcacccaaa cgccattttg cacaaagcgt
     6961 ggtggtaaac ttgccatatt gacaggcgcc ttcatttcaa tcaccccaat cgctccaagt
     7021 actctcattt cagcgacaca atctaaggct tccgcttggc gcaattcttg acgtaattgc
     7081 tgttcaatct gttgaatacg ttgttgccaa gggcttgcca ataacaattt gatactttct
     7141 gctgctaccg cacacgccag tggatttgcc ataaacgtcg gaccgtgcat aaaacatttt
     7201 gcctcgccag aacaaatcac gtctgctacc gcttggttgg taatggtagc ggatagagtt
     7261 aaataccccc ccgttaaagc tttaccgata cacatcacat ccggcacgac attggcatgt
     7321 tcacaagcaa ataatttgcc tgtacgccca aatcctgtgg caatttcatc aaaaatcagc
     7381 aaaatgccgt acggttcgca taaggctttt gcttgcacca aatactcagg ggaataaaaa
     7441 tacataccac ccgccccttg cactaccggt tctaaaatta aacccgcaat ctcatcacca
     7501 tgttgtgcca ataaatcctg taacggttgc atcgcttcgg gttgccaagc ttgcccaaat
     7561 tttaccgggg gttgcggtaa aaaatattgc acgggtagcc ctttggcaaa caagctgtgc
     7621 atgccggtca cgggatcaca cacggacatc gcatgccaag tatcaccatg atagcccgaa
     7681 cgaatcgtag caaatttata gcgctgtgtt tcgcctttag catgttgata ttgcactgcc
     7741 attttcatcg ccacttccac ggcgaccgaa ccactatcgg caaagaaaat tttatccaaa
     7801 ctttccggca agatgttgac cagttgctgc gccagttcta ccgctggttg gtgcgttaat
     7861 ccaccaaaca tcacatgact catttgactc agttgttgcg tggcagcgtg gtttaaacgg
     7921 gggtgattat agccatgaat cgctgaccac cacgatgaca tgccatcaat caactgctga
     7981 ccgtttttta actggatata cacaccttcg gcacgttcta ctgcaaataa gggaaaatcc
     8041 gcatttaacg ccgcataagg gtgccaaata tgttgtcgat cacattctaa aagttgctgc
     8101 tcattcatcg tttcacctcg gttgtgaatc gccctttcaa ctggcgtaaa ttcacgcaaa
     8161 tacttacata aacggaataa cttgttataa tcgccaagca tttggctttt caagcccatt
     8221 attctaactt aaaaagatga tttattacta tgaatatatt agaaaaaaaa gcacaactga
     8281 gttactggca acggattaaa attgcctttc aatatgtgat gccacaactt tatttaacac
     8341 gtcttgcagg ctggtttgca aaacagcaat ggggtgctgt cacacatttt gtgattaaat
     8401 tattcgcgaa aaaataccat gtggatatga gtgaagctgc aaaaccaaat ttcagcgact
     8461 atgcgagctt taatgaattt tttatccgtc cattagcgga caatgcccgt ccaattaatc
     8521 aaaatcctac cgcactttgt ttaccggcag atggtcgtat tagtcagtta ggacacattg
     8581 agcaggattt attgctacaa gccaaaggac attatttcag cttgaacgat ttgttagctg
     8641 gcgacgaggc gctcgctcat cattttaaag atggcgaatt tgccaccacc tacctctcac
     8701 cacgcgatta ccaccgtgtg catatgcctt gtgatgccac cttatgcaaa atgatttatg
     8761 tgccaggtga cttattctca gtcaatccat ttttagccga acacgtgcca aacttatttg
     8821 cacgtaatga acgcgtgatc tgcgtattcg ataccgaatt tggtaaaatg gtccaaattt
     8881 tagtcggcgc aaccattacc gcgagtatga gcaccgtgtg ggcaggtgtg atcaacccgc
     8941 ctcgtccaga aaaaatcacc acctggacct atgaaggaga aagtgcggtc aaattactga
     9001 aaggacaaga aatgggagcc tttcaattgg gctcgacggt gatcaattta tttgaaaaag
     9061 atcgtgtgca actggcatct catttacaag tggatagccc ggtacgtatg ggtgaaattc
     9121 ttgcccacca aaaataattt ttaaccacaa taggaaaaac aatgagtcaa caatttttcg
     9181 accaagtgtc attagaaagc caacttgaag tgagcgccga agccgtcgcg aaagggatct
     9241 ttgatgtgtt aaatcaaaat ccaccagcac ttgagttaaa tgaagtgagt catgccctac
     9301 agactttgca acaacaagcg aaattcaaag aaattgaagc cgttggtgca caattcttag
     9361 ctgaaaacgc gcaaaaagcg ggggtaaata ctacggaatc tggtttacag tatgaagtgt
     9421 tagtggctgg tgaagggcaa attccggcac gcgaagacaa agtgcgtgtg cattacactg
     9481 ggacattaat cgacggtacc gtctttgaca gctcagtaaa acgtggtcaa cctgcagaat
     9541 tcccagtaaa tggcgtgatc gctggttgga ttgaagcctt gtccatgatg ccagtgggtt
     9601 caaaatggcg tttaaccatt cctcacaacc tcgcttatgg cgaacgtggt gctggtgcct
     9661 ccattccgcc attcagtacc ttagtgtttg aagtcgaatt attagccatt ctttaaagct
     9721 aaacgctaga cataaaaaag tgcggtcatt ttccgcactt ttttatgtct agcgtttatc
     9781 gtttcaagcc ttgctgatag ccgctgttcc acgaatggcg gaacttgtcc ggcacagcct
     9841 tgcccgtttg ttcttcacag taagccgttc gttgaatcaa cattttttgt tggtgttctc
     9901 gaaatgattt tcccacacga tagccgtcca aataccaatc atcattatct tgacatgcca
     9961 tggtattaat ccgttttgta ttccccgtca ctgcaaaaga tttgcctgcc tcaatacgtt
    10021 gcaccgcatg ttcagagtat gaagcacagc tcaccagcga acctagtaca ctgaatatca
    10081 atactgcttt ccaattcatc ttgttacctt tctttttctg atttataaca caatcatatc
    10141 gtctcggtgt accgcaaccg ccccatattc ataaccgaga acctgttcga tatcttgcga
    10201 atgtttgcct tgaattaaat ggagcgcatc gctgttataa cgtggcatcc ctaaggcaac
    10261 atccttgcct tgctgagtac gaatttttac cacttcaccg cgtgaaaagc gcccttccac
    10321 gtttacaatc ccagcaggca ataacgattt atgttgtatt aacatcgcgt tttccgcccc
    10381 cgcatcaatg gtaataatac cggctgacgg cgccgcatat agccattgtt tacgactttc
    10441 taagcgatct gcttgcacac taaatttggt gccaatggct tgcccataag ctaagtccac
    10501 aatcacattc gggcgattac ccggtgcaat aatggtttca atgccggagc gggttgccac
    10561 atccgcggca ctgattttgg tggacatgcc acctgtgcct aacgaggtgc cacttccgcc
    10621 cgcaatcgcg cgaatatgat cggtaatttt gtccacgtcg gcgatcaatt tggcgtctgg
    10681 atgtttgcgc ggatcacgat caaataaacc ttgctgatca gtaagcaaat acaactgttc
    10741 tgcttgcacc aaaattgcca ctaacgcgga taaattatcg ttatcgccta ctttaatttc
    10801 tgacgtagcg acagcgtcat tttcattaat cactggaata atctgattgt ctaataaggc
    10861 gcgcaaagta tctctggcat ttaaaaaacg ttcacgatct tcaatgtcgg cacgggtcaa
    10921 taaaatctgc ccaatatgaa tatcataaat ggcaaacaat ttttcccacg tttgaatgag
    10981 ctgactttgt cctaccgcag ccaataattg tttcgaggca atcgtcggtg gcaattgcgg
    11041 gtgatttaaa taatgacgac ctgccgcaat cgcccctgag gtaacaatca ctaaacgaaa
    11101 gcccgcttga tgaagctgtg cgagctgacg cacaatatct atcatatgag gtaaatttaa
    11161 cttcgccgat ccttgcgtaa gcgtactggt tccaaatttt acaacgagtg ttttgtcgat
    11221 tttcatcttc tctatcttct tatttttgtc gcacaaaatt gaagctaact taccattaat
    11281 catcagaaaa atcatctcgt ttacttgcta ttttctatga tttagcacaa attttttctc
    11341 actaaaaggc atataataaa gcttcttgat ttttcagcta ggaacccgat tatgacctca
    11401 acctcttcac aagaaacgga gcggcaacga gaaatcactc gcctttgtgt tcaagttgcc
    11461 caactcttat acaacatggt gcagaaagtg ccatcgttgt gcaaatggca catcgcttag
    11521 gcttagccct cggtgtagat agcgtagaat gtgccctcac gccaaatgcc attattctta
    11581 ctacattagc ccaagaccat tgcatcacga cagtccgtaa aaaccaagac aaaggaatta
    11641 atatgcaagt ggttaccgaa gtgcaacgta ttgtgattac agcagaacgt aaaatctatg
    11701 atatgcaaca agtgcatgcc aaactggctc gcattaaacc gcttaaatac aatcgctatt
    11761 ttgtgatgtt gatgattggc gtatcctgtg ccagttttgc tcatttatct ggtggggacg
    11821 cactagtttc attggttaca tttctcgccg caacactcgc catgtatgtt cgccaatcac
    11881 ttgccgcaag acactataac cccttaattg tatttgcggt gaccgccttt gtcgcctcac
    11941 taattgcggg tatcgcgttg aaatataaca tcggcaatca gccccaaatt gccttggcat
    12001 ccagcgtatt attgctcgtt cccggctttc cactgattaa tgcactcgcc gatattttaa
    12061 aagggcatat caatatgggg attgcccgtt gggcactcgc gacaatcctc acatttgggg
    12121 catgcttagg cattgtattt gcgctgagtt tattcttaga ggaaatctgt atgctaaata
    12181 aattcattca agatgaattt ctacggctag atcaacgtta tgcagcttgg tatcaaagtg
    12241 aagaaacgga tgaagttgcc agaatgggga ttcaagaaag tgagcggatt ttgaaaattc
    12301 tgtatgcact gaaatataac gaagacccta tgcaaaatgg tttaggctat attgcattta
    12361 tggcatcaaa aggccaataa tttacttatc tcaacaattt acccaccggg gaaactcctc
    12421 gatggcgttt cccctacttt ataggagaaa cgctatgact attaacactt atgcaaaatt
    12481 tgcacctacc gtttttgttg caaaatgctc tgaatcacat accaaaggtg aaattattac
    12541 gctaaccagc aaatatggta atgaaaccga ggttcaagta cataatcttg ttaaacaaga
    12601 tggcgagcat tatttttatt catttactcg ttgtgatggg atggatagtc agtcatttgc
    12661 tcaacaaaaa gcagaacgct atcaaggcta tgcagataat gcagccaaac gtagtcaaga
    12721 ttgggtaaat gccgctaatg aggggtgtga ttttttgagt ttaggtgagc caataaaaat
    12781 tggtcatcac agcgaaaaga gacaccgtaa ccttattgaa cgtaatgctc gccgtatgga
    12841 caatgcggtt gcagaaatga acaaagctgc aagttatgaa tctcgtatcg catattggga
    12901 aaaaatggca ggaaaaattg acctgtctat gccggaaagt ctggaatact ttaagtttga
    12961 attggataaa gccaaaacaa agcacaaaga attgaaggac aaccccgaga aacgagagca
    13021 tggttattca ctgacttatg ctaaaaaagc agttaatgaa cttgagaaaa aagtaaaatt
    13081 agctgaatta ctttgggcat aattatttac aaaggagatg acgatatgaa tcgacaaggt
    13141 atttatatta atggcaaaca ggtgtttgat tcacaaggcg aagatattca aagcattgga
    13201 ttttttggct gcaatattca aacggtcgta ggcataaaca aaggaagtat tttagtctcg
    13261 tccaatagta atcaattatt ggtgaatgga cagtcgattg aattaggttc tgagccagtc
    13321 ctcaaaattg aggttttggg caatgtgcag aaactgagtg ccggtgccgc agatgtctct
    13381 attacaggag atgccggcag tgttgacaca gttagcggtg atgtctctat taagggaaat
    13441 gttgcctctt atgtgaaaac cgtgagtggt gatgttcgct gtcgtgctat taccggttcg
    13501 gtggaaaccg tctccggtga tatttatcgc tgatatttac tcatcttatt atttaccacc
    13561 ctattgggaa gcaattttcc caaggtgggc agattgtttc ccatttttat tttgaatttg
    13621 gagaaacatg atgaccacta ttaacctttc acaacaagaa cgtgagttag ttgaatttgt
    13681 ctggaataac cgtaatacac cttggttttt tcactgtagt gtatcgaatg tgtcgagaaa
    13741 taatacctct cggcaaattg ctgtatgtgt cattaaggat aatcaattga ttagccttga
    13801 tgaattgata gcaaaattat gtggttataa actcagacaa aacggattat acgttactgt
    13861 tcataatatg gatgctgttt ttgatacctt atatcgcttt atgtgcaagc taaatgatga
    13921 gatggaggca aatgaaaatg tttgccctta tttatcatta gctcactatg aagtgttttg
    13981 attggtatca gaactatcca ccctgtcggg aaacaatttt tccgataggg cagattgttt
    14041 cccactttta ttttgaattt ggagaaacat aatgatagac accttttttg accttgacga
    14101 cacctcatgc tatttaggta actgggagga tatactgaaa gaatatgatg attgtctttc
    14161 agttgaagat ttttggcatt ccgctaaaga atttgatacc gtacctcatt ttggcaacct
    14221 ataccaggag cgtgtcatcc ttaagttgat tgctcgattc tgtattgagc tgaatattga
    14281 gcaagattca ggtttagtcg aatttgatta ttatatcaat gcaatagata cgcatttcta
    14341 tattaatagc caaagaatct gtgatataga tgattggaat gaaatgttag ataagattcg
    14401 tgaagaaatg acgccggcta aattagttgc ctaatttttg caaattttta ctaaaaatga
    14461 cgcttactgt cgtcttaatt cacccacttg gggaaatatc tccccaatgg gatgacattt
    14521 cccctctttc
//
LOCUS       DANLRK010000051        14495 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.051, whole genome shotgun sequence.
ACCESSION   DANLRK010000051 DANLRK010000000
VERSION     DANLRK010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 14495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14495
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.051"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            56..1501
                     /gene="aldA"
                     /locus_tag="PXA33_001100"
     CDS             56..1501
                     /gene="aldA"
                     /locus_tag="PXA33_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HDL5120439.1"
                     /translation="MVKQHKMYINGEFVPALDGRTLAVYNPATEKAISEVPRGGADDA
                     KIAIDAAEAAQEAWERLPAIERGKYLRQIAVGIRERADEIARTISEEMGKTLSLATVE
                     VHFTADYLDYMAEWARRYEGEIIQSDRPNEHIFLYKKAIGVTTGILPWNFPFFLIARK
                     AAPALVTGNTIVIKPSEDAPNNAILFSEIVHQVGLPKGVFNVVTGYGNEVGPELSGNE
                     KIGMVSFTGSQFAGQKVMEAASKNIIKVNLELGGKAPAIVLDDADLELAATAVVNSRI
                     INSGQVCNCAERIYVQSSIKEQFVQKLVEKMQAVKYGNPLEQQDIDMGPMVNEKGLHK
                     VQEMVTHALHQGGNLVLGGNVVEGTGYYFEPTIIDNVDNSMDIMRHEIFGPVIPVATF
                     DTIDEAIRLANDCEYGLTSSVYTQNINKAMKVISRLKFGETYVNRENFEAMQGYHAGW
                     RKSGIGGADGKHGLEEYLQTQVVYLQFDDKV"
     gene            1757..2404
                     /locus_tag="PXA33_001101"
     CDS             1757..2404
                     /locus_tag="PXA33_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005613280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1440 domain-containing protein"
                     /protein_id="HDL5120440.1"
                     /translation="MSIFQQTNPNRRRYGLAVFIGIIAGIISAFVKWGAEHPFPPRSP
                     IDLFTAACPQPVLDAMNAAADQVAAKTIALQECSRAFLNPPHVFLRDYIGIDPTEAAF
                     TFADHAFNWIGVTHMIFSLVFAIGYCIVAEIFPKIKFWQGIGAGIIANICVHYITFPA
                     LGLTPPVAEWPLYEHISELVGHIFWFWTIEVIRRDLRNRITREPDAEVPLDQPFR"
     gene            complement(2537..3913)
                     /gene="trkA"
                     /locus_tag="PXA33_001102"
     CDS             complement(2537..3913)
                     /gene="trkA"
                     /locus_tag="PXA33_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HDL5120441.1"
                     /translation="MKVIILGAGQVGSTLAENLVSEDNDIVLVDNDPTRLDKLKDKHD
                     LQVIRGEYASPQTLRDAGAGDADLLVAVTNSDEVNMIACQIAYTLFNVPTKIARIRSA
                     DYVRERERLFNDDVLPIDHIIAPEILVKEDILRLIHYPGALQITHFADELVSIVNVKA
                     YYGGPLVGYPISALRDHLPHIDARVVAIFRQDKAIVPQGSTIIEAGDEVFFICATQHI
                     RAVMSELQRLDRPHKRIMIVGGGSIGSSLARDLEDQYRVKIIERNPVRAEKLAEKLSK
                     TMVLTGDASDEELLFEEHIENIDLFLAVTSDDEANIMSALLAKRLGAKKVIILVQRPA
                     YLHLIQGGTIDIAISPQQATISALASYVRKGDILQVSSLKLGVAGAVEIIAHGDATTS
                     KVVGRQIRELKLPQGAIVGAVVRGEEVIIAHKSTMIAENDRVIIFVNDKKQIDEIEKL
                     FQLGVFFL"
     gene            complement(3913..5244)
                     /gene="rsmB"
                     /locus_tag="PXA33_001103"
     CDS             complement(3913..5244)
                     /gene="rsmB"
                     /locus_tag="PXA33_001103"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015431887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HDL5120442.1"
                     /translation="MKKQSFKALSSRAISATIILQVLDQGKSLSTLIPDAQKQLEPKD
                     LPLVQEITFGVCRVLPRLESIIKLLVEKPLKGKTRLVHCLLLVGLYQLLYMRVPAHAA
                     VDEVVNATKTLKLDSFRALTNGVLRRFLREQEEILAKVDKHWQTLHPEWLVNKLKKAY
                     PNWREIVEANNQRPPMWIRVNQQHIKTNDYAALISELVEPEFAKNSENPTACVPKCAL
                     LLERAVNVNQLPHFEQGWATVQDAHAQWSAELLGAENGETVLDACAAPGGKTTHILEK
                     APKAKVMALDIEESRLSRVRENLVRLGQIAQVICGDASKPEEWLEEGVLFDRILLDAP
                     CSATGVIRRHPDIKWLRKEGDIAELATLQGEILKALWQRLKPNGILLYATCSVLPQEN
                     SEQIQRFLQDTPNAKQLEIDFNGEKVLEKQFFPQPNGGDGFFYAKLQKVAE"
     gene            complement(5296..5721)
                     /locus_tag="PXA33_001104"
     CDS             complement(5296..5721)
                     /locus_tag="PXA33_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDL5120443.1"
                     /translation="MYNKLLIAIDLADLKSAKYVVDTALQMTAKNPNAVYRVVSIIEP
                     VDNSLISAFLPKNFDKEVVAEANQKLHEFTKNHFPEGSKVQHIVAYGTIYEEICRIAD
                     EKSVDLIMMLASSKPNAKGLSSNTVKVARNTTKPILVLR"
     gene            complement(5814..8462)
                     /locus_tag="PXA33_001105"
     CDS             complement(5814..8462)
                     /locus_tag="PXA33_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HDL5120444.1"
                     /translation="MSVESKVDYDDLQDMVASNDSGGRTPSGLAKKAIVYVAILWSVF
                     QIYYASPLPFVFQEWLTHAGINLNVVVDDTKARSIHLAFAMFLAFLSYPAFASSPKHH
                     IPKIDWVFAIVGAGLALYYIFFYEGLVKRFGAPNTQDIIAGCLGILLVLEATRRSLGL
                     PLVIIAIVFLIYNFFGQYFPADWIVSHRSGSLSQIINQQWITTEGVFGVALGVSTKYV
                     FLFVLFGALLDKAGAGNYFIKTAFAYLGHLRGGPAKAAVVSSALTGLISGSSIANVVT
                     TGTFTIPMMKRVGFTKEKAGAVEVASSVNGQIMPPVMGAAAFLMIEYVNMPYSQLITH
                     AFLPALISYIALVYIVHLEACKMNLQGLPRAEEPSPILVFLLRTVASILVIVGLAIGV
                     YYGLGWIRDVAEDYAFMIVCAFLGLWYLLAIRRVASYPDLEADDPNSEIVSLPKRKPT
                     VNAGLHYLIPVVVLLWCLMVEMLSPGLSAFWGTITLIFIQLTQRPLLNFFRKESVTKE
                     IIKQGVRDVVDSLEAGGRNMTGIALATATAGIIVGVVALTGFGVQLSSVIELLSMGNI
                     VLMLILVAVFSLILGMGLPTTANYIVVSSLMATVIMEVGRQNGLIVPLIAVHLFVFYF
                     GIMADVTPPVGLASFAAAAISGGSPINTGMVAFKYSLRTVILPFLFIFNTDLLLIDLR
                     YPGHGWVVFITATVGVLAFTSATMQYIVTKNKWWETALLVVAAFALFRPGFFMDRIYP
                     SERHIEPVQFEEQLLASKVGQNVTLKVEGLNPYGKEIEFYAQLPVPEGATGEERLKNL
                     GLTLIQTDEKIKVDGVETPKVVIDMVEMDSPAAKAGLNWDQTIHYIAVPQDAPSKDWI
                     FIPALLLVFGIVANQRRRVKKQCVIA"
     gene            complement(8664..9626)
                     /locus_tag="PXA33_001106"
     CDS             complement(8664..9626)
                     /locus_tag="PXA33_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HDL5120445.1"
                     /translation="MKKLVKLSFLASLVMGTASVQAAETFVTIGTGGQTGVYYVVGQS
                     ICQLVNRDSAKTGLKCNAPSTGASVANLNAIAAKEMDMGIAQSDWQYHAYNGSSSFEG
                     KKNDKIRAIFSLHPEPFTVMARTDSGIKSFDDLKAKRVNVGDPGSGTRATMNVILAAK
                     GWTDKDFKVASELKPAEMASVMCDNNLDAITYNVGHPNGALKEAAASCDAKLVPVTGP
                     EIDKLVADHSYYAKATIPGGLYKGSDEPTETFGVYATLVTSADVDADKVYNVTKAVFD
                     NFDRFKRLHPAFEHLKQEEMIKNALSAPLHEGAIRYYKEKGWIK"
     gene            complement(9810..11657)
                     /gene="selB"
                     /locus_tag="PXA33_001107"
     CDS             complement(9810..11657)
                     /gene="selB"
                     /locus_tag="PXA33_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HDL5120446.1"
                     /translation="MIICTAGHVDHGKTSLLQALTGTNTAHLPEEQKRGLTIDLGYAY
                     LPIENDILGFIDVPGHQRFLSNMLAGLGGIQHALLVISAEEGIKPQTEEHLDILHLLN
                     FQHIMIVITKADRAEETQISHLIEQVKTSYPFLAEAKTFVTSAKTQQGIEELKAHLIQ
                     LNQQHTQTHKPFRYAIDRVFNVKGAGLVVTGTTVAGKVSVGSELFLSNGKKVRVKAIH
                     AQNTPSETGVAGQRLALNIANVEKEEIKRGDWITELEPKFATDRITVQLTANQAFKEN
                     SIVHLYHFASHITGKLNLLEGQQAVKNQQFFAEVILDEPLHIAVGDKLIIRSGDDSLT
                     LAGAEVLEIHSPKRHKRTEARLALVKNLAKTTACNDFATRLDLYLQNRAMDLSLLLWA
                     EQCFAEDIAKLGFDVSSKWLFNSDFKAQAQQRVLEKMAEYHLQHQDQIGVTKARLYRI
                     ALLDLPENLANQFIEDLVEQKALVNSRGWLHLPEHRIELNADELQIWQQIRPLFEATN
                     QALWVRDIASQLSIDETQMRNLLYKVGKLGYLIPIVKDRFLLSEQIGQFATLIKDFIA
                     QNGSISVNQLRDELQYGRKLTVQLIEYFDRSGFLRRKGDVHLLRDSETF"
     gene            complement(11667..11963)
                     /locus_tag="PXA33_001108"
     CDS             complement(11667..11963)
                     /locus_tag="PXA33_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL5120447.1"
                     /translation="MMKFDYNIVWLQKAEIKLFYQADYILQQSQNPIIAEQFYDAIKS
                     EVDKLSFTADVYRLRKKKEIPILNGKYQVKFLIGQENVYIVDFKSARQNSYSFL"
     gene            complement(11960..12136)
                     /locus_tag="PXA33_001109"
     CDS             complement(11960..12136)
                     /locus_tag="PXA33_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5120448.1"
                     /translation="MELILAQDLAKLRTDKPQSFYTNDELDLLHQLENGLIETKSHAQ
                     VMEDLRKKLGITEK"
     gene            complement(12205..12837)
                     /gene="pdxH"
                     /locus_tag="PXA33_001110"
     CDS             complement(12205..12837)
                     /gene="pdxH"
                     /locus_tag="PXA33_001110"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005575912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HDL5120449.1"
                     /translation="MDLHHIREDYTKKVLSIRDCHSNPITQFEQWLSEAINAKVNEPT
                     AMNIATVAANGRPSSRMVLLKEVNSQGFVFFTNYQSRKGWEIADNPFGCLTFFWAELQ
                     RQVRIEGKIAKIPEAESDAYFDSRPYTSRLGAWASEQSQPIENYKALLLKSAQFALKY
                     PLSVPRPPHWGGYILIPDSIELWQGRSSRLHDRIHYWLENNEWKKERLSP"
     gene            complement(12895..14277)
                     /locus_tag="PXA33_001111"
     CDS             complement(12895..14277)
                     /locus_tag="PXA33_001111"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HDL5120450.1"
                     /translation="MQQLFRQIPSLDLLLKKPQAVELCQKFAYSSVAEQARSLLTQAR
                     QFISQQQALPDFIADENLFFIRLEQKVQALSEVKIRSVFNLTGTVLHTNLGRGLWSEK
                     AIQAATNAMRNNVALEFDIEAGKRSHRDNYISELLQILTGAEAACIVNNNAAAVLLML
                     ATFAQGKEVIVSRGELVEIGGAFRIPDIMAQAGCKLVEVGTTNRTHLKDYRNAITENT
                     AFLMKVHTSNYHIEGFTSSVSEEELVALGKEFNLPVITDLGSGSLTDMQALNLPAEPK
                     VQQKVAAGVDLVSFSGDKLLGGPQAGIIVGSQAMISQLQQHPLKRVLRCDKVILSALE
                     ATLRHYLFPEQLTDNLPTLHLLTQPLETLQHKAERLKQALHKRLNSAYILQIELSLAQ
                     IGSGALPTETLPSVAVTISAEKQSDLIALEQQFKTYPSPIIGRFAQQKFWLDLRSVAE
                     FDKLIEMLEY"
     gene            14345..14439
                     /locus_tag="PXA33_001112"
     tRNA            14345..14439
                     /locus_tag="PXA33_001112"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14379..14381,aa:Sec,seq:tca)
BASE COUNT         4119 a         3271 c         2904 g         4201 t
ORIGIN      
        1 tcagttaccg aatgatagtc atcaacttcc actgtggata aaaaaggagc aactcatggt
       61 gaaacaacat aaaatgtata ttaatggtga atttgtgcct gcgttagatg gtcgcacttt
      121 agcagtctat aaccctgcca ccgaaaaagc tatctccgaa gtccctcgtg gcggagctga
      181 tgatgccaaa atcgctatcg atgctgctga agccgcccaa gaagcttggg aacgtttacc
      241 agcgattgaa cgaggaaaat atttacgtca aattgctgtg gggattcgtg agcgtgccga
      301 tgaaattgct cgcactattt ccgaagaaat gggtaaaact ctgtccttag caaccgttga
      361 ggtacatttt accgctgact acttagacta tatggcagaa tgggcgagac gttatgaagg
      421 ggaaattatc caaagtgatc gcccaaatga acatattttc ctatacaaaa aagcgattgg
      481 cgtcaccacc ggcattttac cttggaactt cccgttcttc ctcattgccc gtaaagcggc
      541 tccggcatta gtaacaggta atactattgt gattaaaccg agtgaagatg cacctaataa
      601 tgcgattctc ttttcggaaa tcgtgcatca agtcggttta cctaaggggg tatttaatgt
      661 cgtcaccggc tatggcaatg aagtcgggcc ggaactttca ggcaatgaaa aaatcggtat
      721 ggtttccttt accggtagcc agtttgccgg acaaaaagtg atggaagccg ccagcaagaa
      781 tatcattaaa gtcaatttgg aattgggcgg aaaagcaccg gctatcgtat tagacgatgc
      841 agatcttgag cttgctgcta ccgctgtagt aaattctcga atcatcaatt caggacaggt
      901 ttgtaactgt gcagaacgca tttatgtaca gagttcaatt aaagagcaat ttgtccaaaa
      961 attggtagag aaaatgcaag cggtgaaata tggtaatcca ttagaacaac aagacattga
     1021 tatggggccg atggtaaatg aaaaaggctt gcataaagtg caggaaatgg tgacacacgc
     1081 actgcatcaa ggaggtaatc tggtattagg tggtaacgtg gttgaaggaa caggctacta
     1141 ctttgagcca actattatcg acaatgtaga taactctatg gatattatgc gtcacgaaat
     1201 ttttggtccg gtcattccgg ttgcgacatt tgataccatt gatgaagcga ttcgcttagc
     1261 caatgattgt gaatatggct taacctcttc ggtttacacg caaaatatca ataaagcgat
     1321 gaaagtaatt tcccgcttaa aattcggaga aacttatgta aaccgtgaaa actttgaagc
     1381 aatgcaaggc taccacgccg gttggcgtaa atcgggtatt ggcggtgctg acggtaagca
     1441 cggcttagag gaatacctac aaactcaggt ggtttatctg caatttgatg ataaagtata
     1501 gattcttcta aaaactccca cttatcaaaa taagcgggag ttttctttta ggttaacctt
     1561 gtatcaaaat tgcaaacaaa aatattctaa tgttaagtaa atgtttaata attatttatg
     1621 gttaactctt tttggctaat taatttgcaa tttaaattaa ttgagtgcag aatgccatta
     1681 gccttttttg aaatcacatt ccataaaatg gcttgattaa cacaacacaa ttatcactca
     1741 aattaggatt ttcattatga gtattttcca acaaaccaat ccaaaccgcc gtcgttatgg
     1801 gctagctgta tttatcggga ttattgccgg tattatttca gcattcgtaa aatggggggc
     1861 agaacatcct ttcccacctc gtagccctat cgatctgttt accgctgctt gcccgcaacc
     1921 tgtgttagat gcaatgaatg ccgcagcaga ccaagtggct gccaaaacca tcgcattaca
     1981 agaatgttca cgtgcatttt taaacccacc acacgttttc ttacgtgatt atattggtat
     2041 cgatccaact gaagcagcat ttacctttgc tgaccacgcg ttcaactgga ttggtgtcac
     2101 ccatatgatt ttctcgttag tctttgcgat tggttactgc attgtggcgg aaatcttccc
     2161 aaaaattaaa ttctggcaag gtatcggtgc cggtatcatc gctaatattt gtgttcacta
     2221 catcaccttc ccggcattag gtttaacacc accggttgct gaatggccgt tatatgaaca
     2281 catttctgaa ttagttggtc atattttctg gttctggacc attgaggtga ttcgtcgcga
     2341 tttacgtaac cgtattacgc gtgaacctga tgccgaagtg ccgttagatc aaccgttccg
     2401 ttaatttaaa attagctcga tttatacaaa agccgcaatc acgttgcggc tttcttattt
     2461 tacaagcggt caaattttca tgaaaattta ccaaacaaaa agcacattgt ttcatgtagc
     2521 aatgcggagc ttggctttat aagaaaaata caccaagttg gaacagtttt tcaatttcat
     2581 cgatctgttt cttatcgttc acaaaaataa tcacacggtc attttctgca atcatggtgg
     2641 atttgtgggc gataataact tcttcgcctc gcaccaccgc cccaacaatt gccccttgcg
     2701 gtagttttaa ttcacggatt tgtcgtccaa ccacttttga agtggttgca tcgccgtgag
     2761 cgataatttc taccgctcct gcaaccccaa gtttgagtga agaaacttgt aaaatatcgc
     2821 cttttctaac gtaactagcg agagcagaaa tagtagcttg ttgtggggaa atggcaatat
     2881 caatagtgcc accttgaatc aggtgtaaat aggcagggcg ttgcactaaa ataatcactt
     2941 ttttcgcccc aagccgtttt gccagcaggg cggacataat attcgcttca tcatcactgg
     3001 tgacggcaag gaataaatca atattctcaa tatgctcctc aaacagcaac tcttcatcgg
     3061 aggcatcacc ggtaagcacc atcgttttag atagtttttc tgctaatttt tcggctcgaa
     3121 ccggattgcg ttcgataatt tttacccgat actgatcttc taaatcccga gctaacgaag
     3181 agccgatgct ccctccaccg acaatcataa tccgcttatg tgggcgatca agccgttgta
     3241 gttcactcat tactgctcga atatgttggg ttgcacagat gaaaaagact tcatcacctg
     3301 cctcaataat agttgaacct tgtggcacaa tcgctttgtc ttggcggaaa attgccacga
     3361 cccgagcatc aatatgcggt aaatgatcac gcagggctga aatcgggtaa ccgaccaaag
     3421 gcccaccgta ataggctttt acattcacga tactgactaa ctcatcagca aaatgagtaa
     3481 tttgcaacgc ccccggatag tgaataaggc gtaaaatatc ttctttgact aaaatttcag
     3541 gggcaataat atggtcaata ggcaatacat catcattaaa caaccgctct cgttctctca
     3601 cataatcagc actgcgaata cgggcaattt tggtcggcac attaaataaa gtgtaagcaa
     3661 tttggcaagc gatcatattc acttcatcac tattagttac agcaactaat agatcagcat
     3721 cacctgcacc ggcatcacgc aaggtttgag gtgaagcata ttcgccccga atcacctgta
     3781 aatcgtgttt atctttgagc ttgtctaaac gagttgggtc attatccacc aagacaatat
     3841 cattgtcttc actcaccaaa ttttctgcca gcgtagaacc aacttgtcct gcccctaaaa
     3901 taattacttt cattactctg ccactttttg tagttttgcg tagaagaaac catcaccacc
     3961 gtttggttgt gggaagaact gtttttctaa cactttttcg ccgttaaaat cgatttcaag
     4021 ttgttttgca ttcggtgtat cttgtaaaaa ccgttgaatt tgctcgctat tttcttgcgg
     4081 taacaccgag caagtggcat ataacaaaat gccgtttggc tttaaccttt gccataacgc
     4141 cttgagaatc tcaccctgta atgtggctaa ttctgcaata tcaccctctt tacgcaacca
     4201 tttaatatca gggtggcggc gaatgactcc ggttgcggaa caaggggcat caagtaaaat
     4261 gcggtcaaac agcacaccct cttcaagcca ttcctcaggt ttggaagcat cgccacaaat
     4321 tacctgtgca atctgcccca gtcggactaa gttttcacgc actcggctta aacggctctc
     4381 ttcaatatcc aacgccataa cctttgcttt cggtgctttt tctaaaatat gggtagtttt
     4441 gccacccggt gcggcacagg catctaaaac ggtttcacca ttttctgccc cgagcaactc
     4501 cgctgaccat tgtgcgtgag catcttgcac ggttgcccaa ccttgctcga aatgtggcag
     4561 ttggttcaca tttaccgctc tctccagcaa taaagcacac tttggcacgc aagcggtcgg
     4621 attttctgaa ttttttgcaa attcaggctc aaccagctcg ctaatcaacg ccgcataatc
     4681 attggtcttg atatgttgtt gattgactcg aatccacatt ggtgggcgtt ggttgtttgc
     4741 ttcaacaatt tctcgccagt tcggataggc ttttttaagt ttattgacca accattcagg
     4801 gtggagcgtt tgccaatgtt tatctacctt cgctaagatt tcttcttgct ctcgcagaaa
     4861 acggcgtagc acaccgttag tcaaggctcg gaagctatcc agttttagcg ttttagttgc
     4921 attcactacc tcatcaaccg cagcgtgtgc cggcactcgc atataaagca gttggtataa
     4981 acccactaac agtaagcaat gcaccaaacg ggttttgcct ttcagcggtt tttcaactaa
     5041 taatttgatg atcgattcca agcgaggcag cacacggcac actccgaagg tgatttcttg
     5101 cactaagggt aaatcttttg gttcaagctg tttttgagcg tcaggaatca aggtggaaag
     5161 tgatttacct tgatctaaca cctgtaaaat aatggttgca cttattgctc tagaagaaag
     5221 tgctttgaac gattgttttt tcataaataa aaatgccatt ccgacagaat ggcactattg
     5281 taactgaatg cgggattatc ttaaaactaa aattggcttg gtggtgttgc gagccacttt
     5341 aaccgtatta gagcttaagc ccttcgcatt tggtttgctg cttgccagca tcataattaa
     5401 atccaccgac ttttcatcgg caatacggca aatttcttca taaatcgtac catacgccac
     5461 aatgtgctgc acttttgaac cttcaggaaa atggtttttg gtaaattcgt gcaatttttg
     5521 gtttgcttca gccaccactt ctttatcgaa atttttcggc aagaatgcag aaattaagct
     5581 gttatccacc ggctcaataa tagacacaac ccgataaacc gcattcggat tttttgccgt
     5641 catttgtaat gccgtatcta ccacatattt agcacttttt aggtcggcta aatcaatcgc
     5701 aataagtaat ttgttataca tatttattcc gtaggggcag gtcctgtgcc tgcccgaata
     5761 ttaactaaaa tagcggacgg gcacaggacc cgtccctaca tagcatataa atactatgca
     5821 ataacacact gttttttcac tctacggcgt tggtttgcca caataccaaa taccaacaat
     5881 agagcaggaa tgaagatcca atctttagaa ggagcatctt gcggtacggc aatgtagtga
     5941 atggtttgat cccagtttaa gcctgcttta gctgccggag aatccatttc taccatatca
     6001 atcaccactt ttggtgtttc tacgccatca actttgattt tttcatcggt ttgaattagc
     6061 gttaagccta agtttttcaa acgctcttcg ccggttgctc cttccggtac aggtaattga
     6121 gcgtagaact caatttcttt accgtaaggg tttaatccct ctacttttaa agtaacattt
     6181 tgacctactt tgcttgcaag caactgttct tcaaactgca ccggctcgat atgacgttca
     6241 gacggataaa ttctgtccat aaagaagccc ggacggaaca aggcgaaagc ggctacaacc
     6301 agcaacgccg tttcccacca tttgttttta gtcacaatat attgcattgt agcagaggta
     6361 aaggcgagaa ccccaacagt tgcggtaata aacacaaccc agccgtgccc cggataacgc
     6421 agatcaatta ataataaatc ggtattaaaa ataaataaga acggtaaaat gacggttctt
     6481 aaactatatt taaatgccac cataccggta ttaatcgggc taccgcctga aattgctgcc
     6541 gcagcgaacg aggctaaacc aacaggtgga gtcacatctg ccataatccc aaaatagaat
     6601 acgaataaat gcaccgcaat caacggcacg attaacccgt tttgacgacc gacttccatg
     6661 ataaccgttg ccattaatga tgacaccaca atgtagtttg cagttgtcgg taagcccatt
     6721 cccaaaatta agctgaatac ggctaccaag atcaacatta gcacaatgtt gcccattgat
     6781 aggagttcaa tcacacttga aagctgaacc ccaaagcccg ttagtgccac aacgccgaca
     6841 ataatccctg ctgttgcggt agcgagggcg ataccggtca tattacgacc gccagcctca
     6901 aggctatcta ccacatctcg aacaccttgt ttgataatct ctttggttac gctctcttta
     6961 cggaagaaat tcagtaacgg gcgttgggtt aattgaataa aaattaaggt aatggttccc
     7021 cagaatgcgg ataagcccgg cgaaagcatt tccaccatta agcaccaaag tagcacaacc
     7081 acaggaataa ggtaatgtaa acctgcattt acggttggtt ttctttttgg tagagaaaca
     7141 atttctgagt ttggatcgtc cgcttctaaa tcagggtagc ttgccacacg gcgaatagcc
     7201 agtagatacc ataagcctaa gaaagcacag acaatcataa acgcataatc ttctgccaca
     7261 tcacgaatcc aacctaagcc gtagtaaacg ccaatcgcta atcctacgat cactaaaatt
     7321 gaagctacgg ttcttagcaa gaagactaaa atcgggcttg gctcttcggc tcttggtaag
     7381 ccttgtaaat tcatcttaca agcttctaaa tgcacgatat acaccaacgc aatatacgaa
     7441 attaacgcag gtaagaaggc gtgagtaatc agctggctat acggcatatt cacatattcg
     7501 atcatcaaga acgctgcagc ccccattacc ggtggcataa tttgaccgtt tactgatgag
     7561 gcaacttcca ccgcaccggc tttttctttg gtaaagccga cccgtttcat cattggaata
     7621 gtgaatgtac ctgtcgtcac gacgtttgca atcgaagagc ctgaaatcaa gccggttaaa
     7681 gcggaagaaa ccaccgccgc ttttgccgga ccaccacgca aatgccctaa ataggcaaag
     7741 gcggttttaa taaagtagtt acctgctcct gctttatcga gcaacgcccc aaataatacg
     7801 aacaggaaca catatttggt cgaaacccca agggctacac caaacacgcc ttcagtggta
     7861 atccattgtt ggttgataat ttgagaaagc gaacctgagc ggtggcttac aatccaatct
     7921 gccgggaaat attgcccgaa gaagttatag atcaggaata caatcgcaat aatcacaagc
     7981 ggtaagccaa gactacggcg ggtcgcttcc agcaccaata aaatccctaa acagccggcg
     8041 ataatatctt gcgtatttgg cgctccaaaa cgtttcacca aaccttcata gaagaaaata
     8101 taatacaacg ctaacccagc tcctacgatg gcaaacaccc aatctatttt aggaatatga
     8161 tgtttaggcg agctggcaaa agcagggtaa gataggaacg ccaagaacat tgcaaaggct
     8221 aagtggatag aacgggcttt ggtgtcgtcc accaccacat tcaaattaat gcctgcgtga
     8281 gtaagccatt cttggaatac aaacggcaat ggagaagcat aataaatttg gaacacagac
     8341 caaagaatgg cgacatacac aatggctttt ttcgcaagac cggacggggt tcgcccccca
     8401 ctgtcattgg aggcaaccat atcttgtagg tcatcgtaat caaccttaga ttcaactgac
     8461 ataaagactc ctcaaaacgc aagcggtcat tttcttatta aattttacaa atttcctcaa
     8521 aaaacaaccg cttgctcgtg aaaacactgt tgataagtta tcaattaaag gtgaaaatcg
     8581 ataacttatc aacagtttca aaaaagcaaa aaaccagcac gctaaaaaac gtgctgattg
     8641 aatcaaatta cagaaaggaa atattactta atccaacctt tctctttata gtaacgaatt
     8701 gcaccttcgt gtaacggagc agaaagggcg tttttgatca tctcttcttg ttttaagtgt
     8761 tcaaacgccg gatgtaaacg tttgaaacgg tcgaagttat caaataccgc tttggttaca
     8821 ttgtacactt tatctgcatc cacatcggca gaagtcacta acgttgcata tacaccgaag
     8881 gtttctgtcg gctcatccga acctttatat aaaccgcccg gaatcgtagc tttggcataa
     8941 tatgagtgat ctgccactaa tttatcaatt tcagggccgg ttaccggcac taatttcgca
     9001 tcgcaagaag cggctgcttc ttttaacgca ccattcgggt gacctacgtt ataagtgatg
     9061 gcatctaagt tgttatcaca cattacagat gccatttctg ccggtttcaa ttcagaagcc
     9121 actttgaaat ctttatccgt ccagcctttt gccgctaaaa tcacgttcat tgttgcacga
     9181 gtaccggaac ccgggtcgcc cacgtttaca cgttttgctt ttaaatcatc gaacgattta
     9241 atgcctgaat cggtacgagc cattactgta aacggttccg ggtgaagcga gaaaatggca
     9301 cgaattttat cgtttttctt accttcaaat gagcttgagc cgttataggc gtggtattgc
     9361 cagtctgatt gtgcaatccc catatccatt tcttttgctg caatcgcatt taagtttgca
     9421 accgatgccc ctgttgaagg agcgttacat tttaaacctg tttttgctga atcacggttt
     9481 actaattggc aaatagattg acccacaaca taataaacac cggtttgacc gcctgttccg
     9541 atagtcacaa atgtttcagc cgcttgaaca gatgctgttc ccatcacaag gcttgcaagg
     9601 aaagaaagtt tgactaattt tttcattttt aactcctaaa attaaggatc agaaaacaaa
     9661 gatttattgt tttgagccac tgctcacact acaagagata caaaacttct ttgagttgca
     9721 tttgcctctt agagcttgtt tttagcatag attttataat tagtaaattt attttttcgc
     9781 ataaaaaaat ctatgcctaa tgccacaagc taaaatgttt cactatctct taacaaatgc
     9841 acatcgcctt ttcgtcttaa aaaaccgcta cgatcaaaat attcaattaa ttgtaccgtc
     9901 aattttcgcc catactgcaa ctcatctcgc aactggttca ccgaaatgct gccgttttgg
     9961 gcgataaaat ctttaattaa cgttgcaaat tgcccgattt gttcgcttaa taaaaatcta
    10021 tctttgacga ttgggattaa atagcccaat ttcccgactt tatacagcaa attccgcatt
    10081 tgggtttcat caatcgagag ctggcttgca atatccctca cccaaagagc ttgattggtt
    10141 gcctcaaaca gcggtcgaat ttgttgccaa atttgcaatt catcggcgtt caattcgatt
    10201 cgatgttccg gcaaatgtaa ccaaccacga ctattgacca aggctttttg ctccaccaaa
    10261 tcctcaataa attgatttgc caagttttca ggcaaatcca ataacgctat gcgatataaa
    10321 cgagctttgg tcacgccgat ttgatcttga tgttgcaggt ggtattctgc cattttttcc
    10381 aacacccgtt gctgggcttg agctttaaaa tccgaattaa ataaccattt agacgagaca
    10441 tcaaacccca gttttgcaat atcctctgca aaacattgct ccgcccataa cagcaaggat
    10501 aaatccatcg ctctgttttg cagatagaga tcaaggcgtg tcgcaaagtc attacaagcg
    10561 gtggtttttg ctaaattttt taccaaagct aaacgggctt cggtacgttt atgccgtttt
    10621 ggtgagtgaa tttccagcac ctccgctccg gctaacgtta ggctgtcatc accgctgcgg
    10681 atgatcaact tatcaccaac ggcaatatga agtggttcat ctaaaatcac ttctgcaaaa
    10741 aattgctgat ttttgaccgc ttgttgcccc tcaagcaaat tcaatttgcc ggtaatgtgc
    10801 gaagcaaaat ggtaaagatg cacaatgctg ttttctttga aggcttgatt ggcagtgagt
    10861 tgaaccgtaa tgcgatcggt ggcaaatttc ggctcaagct cggtaatcca atcgcctcgc
    10921 ttgatttcct ctttttccac gttagcgata tttaacgcca aacgttgccc tgccactccc
    10981 gtttccgaag gcgtattttg ggcgtgaatc gctttgaccc gcactttttt accattcgac
    11041 aaaaataact cagagccaac gctcactttt cccgccaccg ttgtgccggt aacgaccaac
    11101 cctgctcctt tcacattaaa cacacggtca atcgcataac ggaacggttt atgggtttgg
    11161 gtatgttgtt gatttaattg aattaaatgt gcttttaatt cttcaatccc ttgttgcgtt
    11221 tttgccgaag taacaaaggt ttttgcttcc gctaaaaacg gatagcttgt tttcacttgc
    11281 tcaattaagt ggctgatttg agtttcttcc gcccgatctg ccttagtaat cacaatcata
    11341 atatgctgga aattcagcag gtggagaata tccaaatgct cttcggtttg tggcttaatc
    11401 ccttcttcgg cagaaatcac caacagggcg tgttgaatgc cgccaagccc tgccagcata
    11461 ttagataaaa aacgctgatg acccggcaca tcaataaacc ctaaaatatc gttctcaatc
    11521 ggtaaatagg catagcccaa atcaatggtt agcccacgtt tttgttcttc gggcaaatgg
    11581 gcggtgttag tgccggttaa ggcttgtaaa agcgatgttt tgccgtggtc aacgtgaccg
    11641 gcggtgcaga tgatcattga tagtccttat aaaaatgagt aactattctg gcgagcagat
    11701 ttaaaatcta caatatagac attttcctga ccaatcagga actttacttg gtacttaccg
    11761 tttaaaatgg gaatttcttt tttcttgcga agtcgataaa catcggcagt aaaggaaagt
    11821 ttatccactt cactttttat tgcatcataa aattgttctg caataatcgg gttttgggat
    11881 tgttgtaata tataatctgc ttgataaaac aattttattt ctgctttctg tagccaaaca
    11941 atattgtaat caaacttcat catttttccg tgattcccaa ttttttgcgt aaatcttcca
    12001 tcacctgagc gtgggattta gtttcaatta agccattttc taattgatgt aataaatcaa
    12061 gttcatcatt agtataaaaa ctttgtggct tatctgtgcg taattttgct aaatcttgtg
    12121 cgagtatgag ttccatattt cccccttaca aacttagatt attcaatatt gtaacctgat
    12181 aaaactaaag ttcaaggagt gtatttaagg cgataaccgc tcttttttcc actcattatt
    12241 ttccaaccaa taatgaatgc ggtcgtgtag ccggctactt ctgccttgcc agagttcaat
    12301 actgtcggga atcagaatat agccacccca atgcggtggg cgaggcactg aaagtggata
    12361 tttcaatgca aattgagctg attttagcaa caaagccttg tagttttcga taggctgact
    12421 ctgctcgctc gcccaagccc ccaaacggct ggtgtaagga cgagagtcaa aataggcatc
    12481 ggattctgcc tctggaattt ttgctatttt cccttcaatc cgcacttgac gttgtaattc
    12541 tgcccaaaag aaggttaaac atccaaaagg attatcggca atttcccacc ctttacggct
    12601 ttgataattg gtaaagaaca caaagccttg tgaatttacc tctttcaaca gcaccattcg
    12661 gctacttggt cgcccgtttg ctgcaacggt agcaatattc attgctgtcg gctcattcac
    12721 tttggcattg attgcctcac tcaaccattg ttcaaattgg gtgattgggt ttgagtgaca
    12781 atctcggatt gaaagcactt ttttggtgta atcttctcga atgtggtgca gatccataag
    12841 gattccttta tcttattcta aataatcgga caggcacagt ctgccctacc aaaattaata
    12901 ttccaacatc tcaatcaact tatcaaactc cgcgaccgag cgtaaatcta accaaaattt
    12961 ctgctgagca aaacgcccga taatcgggct tgggtaggtt ttaaattgtt gttccaatgc
    13021 aattaaatcg ctctgttttt cagcggaaat agtcactgcc accgaaggta aggtttctgt
    13081 gggtaacgca ccgctgccga tttgagcgag gcttagctca atttgcaaaa tataagcaga
    13141 atttaaccgc ttgtgtagtg cctgttttaa tcgctctgct ttgtgttgca aggtttcaag
    13201 cggttgagtg agtaaatgaa gagtcggcaa gttatcggtt aattgctccg gaaataagta
    13261 gtggcgtagc gttgcctcaa gtgccgataa aatcacttta tcgcaacgta gcacccgttt
    13321 tagcgggtgt tgttgcagtt ggctaatcat cgcttggcta ccgacaataa tgccggcttg
    13381 cggcccgcct aataatttat cgccagagaa ggaaaccaaa tctaccccag ccgctacttt
    13441 ttgctgaacc tttggctcag caggcaaatt aagagcttgc atatcagtta gcgaaccact
    13501 gcctaagtcg gtaatcacag gcaggttaaa ctctttaccg agagccacca attcctcttc
    13561 cgacaccgaa ctggtgaacc cctcaatgtg gtagttactg gtgtgaactt tcattaaaaa
    13621 ggcggtattt tcggtgatcg cgttgcgata atcctttaaa tgagtgcgat tagtcgtacc
    13681 aacttccacc aatttgcaac cggcttgagc cataatatcc ggaatgcgga atgccccgcc
    13741 aatttccacc agctcgccac gggaaacgat gacttccttg ccttgtgcga aggttgctaa
    13801 catcaacaac accgctgccg cattattatt cacaatgcaa gcggcctccg ctccggtaag
    13861 tatttgcaac aactcggaaa tgtagttatc tcggtgcgag cgcttgcctg cttcaatatc
    13921 aaattccaac gccacattgt ttcgcattgc gttggttgct gcctgtatcg ccttttccga
    13981 ccacaaccca cgccctaagt tggtgtgtaa caccgtaccg gttaagttga acacggaacg
    14041 gattttcacc tcactcaacg cttgcacttt ttgttccagc cttataaaaa acaggttttc
    14101 atcggcaata aaatccggca aggcttgctg ttgggaaata aattgacgtg cttgagtaag
    14161 caaactccta gcttgttcag ccacgctgga atatgcaaat ttttggcata attcgaccgc
    14221 ttgtggcttt ttcaacaaga ggtcgagcga gggaatttgg cgaaatagtt gttgcataat
    14281 gtgaatgatt gagagaatat tttggacaat tctaataaat aaagtacaat attgccaatt
    14341 tttcggaagt gcgtcatctc cggtgaggtg gcaggacttc aaatcctgtt gaggacgcca
    14401 gcgttctcgg gtgggttcaa ctcccatgca cttccgccac atttcaaaag ccgttaatct
    14461 ttaacggctt tttgcatttc tgccacattc tctct
//
LOCUS       DANLRK010000052        14368 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.052, whole genome shotgun sequence.
ACCESSION   DANLRK010000052 DANLRK010000000
VERSION     DANLRK010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 14368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14368
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.052"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(135..1682)
                     /locus_tag="PXA33_001113"
     CDS             complement(135..1682)
                     /locus_tag="PXA33_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HDL5120451.1"
                     /translation="MYLKHIEISGFRGINHLSLQLRPNMVLIGENAWGKSSLIDALSL
                     IFNLEQALYQFQFKDFHIQYTDTPNQVRDIQLVFTFCEEHQHDLAVQFPAYLAQAAFY
                     IDNHRCVSLAVTGKLHGEQISTDYQFVDQSLKPIQIEQAENAIRQFIYHHPVYRLRDA
                     RLNNRVHNELLLKGSSQLEDDFQRELNALSSLLKYYFLTSQSRYLIKTSMQDTALWWE
                     KVKSLCLRLKQQPELVESAQQHISTLFLFNPRSTVIEKPIILFEDLNAQLHPRMIAIF
                     WELLGFLPSQRITTTNSIELLSQVPLREICRLVRHHDHTQAYWLERHSLGKEDLRKLT
                     FHIHHNRGLALFAKAWILVEGETEVWILQELARLLDIHLEMEGIKIVEFAQCGLRPLI
                     KYARAMGIEWYVLTDGDDSGKRYADTVKSMEEDFAHRLTMLPQKDIEHFFYKAGLRNV
                     FIQLANWRSQEKYPVSYIIKRAIQRSSKPDLAIALANEMGQRGEHSIPTLFKQLFADV
                     LALINKV"
     gene            complement(1702..3069)
                     /locus_tag="PXA33_001114"
     CDS             complement(1702..3069)
                     /locus_tag="PXA33_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HDL5120452.1"
                     /translation="MAIHRVNPKGSMEQLSHLEMELLAKNTQGNLHQLYRNCSLAVLN
                     SGVHTDDSRALLSQYPDFEIRLLTREKGVSLELHNPPETAFVDGKMILNIQYHLFAVL
                     RDIVFVNALKNAIRPLEETSLEALTTNTVFSILRNAKAIEMNTDPNLVVCWGGHSINE
                     TEYQYCRAVGQEFGLRELNIVTGCGAGVMEAPMKGAAIGHANQRYKNGRFIGITEPSI
                     IASEPPNPIVNELVIMPDIEKRLEAFVRLGHAIVVFPGGPGTLEELLYIIGIKLNPAN
                     HAQQLPLILTAPKESADYFYAIDQFIGETLGEEARSLYEIIIDDSVLVARKVKQAMIE
                     VKSSRYESGDAYNFNWSLKIEEDFITPFIPTHENMANLNLSLAQPKEQLAANLRRAFS
                     GIVAGNIKPETSDLIEKYGVFKLHGDRKLMEHIDKLLERFVVQHRMKIPTGEAYQPCY
                     EIVKG"
     gene            complement(3116..3874)
                     /gene="tcdA"
                     /locus_tag="PXA33_001115"
     CDS             complement(3116..3874)
                     /gene="tcdA"
                     /locus_tag="PXA33_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HDL5120453.1"
                     /translation="MTERLDNYEQRFGGIGRLYTPEGLEKLRRSHICVIGIGGVGSWA
                     VEALARSGIGKITMIDMDDICVTNINRQIHAMSGTVAQLKTEAMKERIERINPECVVE
                     IIDDFLTQDNLADYLNRGYDYVIDAIDSLRVKAAMIAFCKRNKIKIITTGGAGGQTDP
                     TKIQITDLSKTVQDPLAARVRSLLRKEYGFSNKNKFGVECVSSTQQLIFPQMGEACEV
                     SATMNCANGFGAVTMVTATFGFFAVSRVIDKLLK"
     gene            complement(3956..5920)
                     /locus_tag="PXA33_001116"
     CDS             complement(3956..5920)
                     /locus_tag="PXA33_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL5120454.1"
                     /translation="MALINLSNAYLGFGDHPLLDHTELHIEPGERVCLVGRNGAGKST
                     LMKILAGEVQLDDGKLVFEKDIVVTRLEQDPPRHIEETVFDYVAEGIAHLADLLKQYH
                     HISLQMQTDYSDELLAKLSAVQSQLEHANGWQFENRIQDTLKLLELAPDKRLCELSGG
                     WVRRAALARALVADPDVLLLDEPTNHLDIEAIAWLENLLLDFKGSIIFISHDRAFIRK
                     MATRIVDLDRGKLVSYPSNYDKYLEEKAEDLRVEALQNELFDKKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKALREERRNRREVQGTAKIQIDNTARSGKIVFEVENAGYEIGGRTL
                     LKNFSTTILRGDKIALIGANGIGKTTLIKLLLGEIQPTSGSVRCGTKLEVAYFDQYRV
                     DLDPEKTVMDNVADGKQDVEVNGVKRHVLGYLQDFLFPPKRAMTPVKALSGGERNCLL
                     LAKLLLKPNNLLILDEPTNDLDVETLELLEEMLTDYQGTLIIVSHDRQFVDNTVTECY
                     FFEGEGVVNKYVGGYFDAKQQQENYFAATADKRLNSSKNLQKIEEKAPLAEQEKQKAD
                     QEKTKAEPTKKTKLSYKEQRELDELPAKMEQLEADIEALQAEVNSAEFFAKDPSYTNA
                     QLQKLADTEAALEQAFERWEALENIKNGNI"
     gene            complement(6015..8711)
                     /gene="gyrA"
                     /locus_tag="PXA33_001117"
     CDS             complement(6015..8711)
                     /gene="gyrA"
                     /locus_tag="PXA33_001117"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HDL5120455.1"
                     /translation="MSELAKDIIPVSIEDELKTSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLFSMDQNSNTYNKPHVKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDAPAAMRYTEVRMQKITQELLTDLDKETVDFSPNYDGKEMIPDVLP
                     TKIPALLVNGSSGIAVGMATNIPPHNLGEVLDGCLAYIENEQISVEELMRYIPGPDFP
                     TGAIINGRKGIEEAYRTGRGKVYVRAKASVETTDKGREQIVVTELPYQVNKAKLIEKI
                     AELVKEKKIEGISKIDDYSDKKGISIVIEVKRDAVGEVVLNHLYSLTQMQVTFGINMV
                     ALDNGQPKVLNLRQIIEAFVKHRREVVTRRTIFELRKARERAHILEGLAIALANIDPV
                     IELIRASKTAEEAREGLLARPWALGNVAPMLEAAGVGASRPEDLPEHLGVRDGEYYLS
                     EAQARAILELRLHRLTGLEHEKIVEEYKELLTEIGELIHILTSPERLMEVIREELERV
                     KTDFNDERRTEITAASGDINIEDLIAQEDVVVTLSHAGYVKYQPLSDYEAQRRGGKGK
                     SATKMKEDDFIEKLLVANTHDTILCFSSRGRLYQLKVYQLPQASRGARGTPIVNILPL
                     VKEENERITAILPIPNGEFSADKFIFMATASGVVKKVSLDAFSNVRASGLIALKLREG
                     DELIGVDITDGNSEIMLFSAQGRVVRFAETAVRAMGRTATGVRGIKLSTTEISSEEIE
                     EAEIIETDNEEAEDSSSVSLDKDRVVSLVIPRTEGDILTVTQNGYGKRTVISEYPVKS
                     RATKGVVSIKVNERNGKVVAAVQVEETDQIMLITDAGTLVRTRVAEVSLVGRNTQGVR
                     IIRTAEDEQVVSLERVCEPEDDENESLDALENAETAETSTEE"
     gene            complement(8808..9188)
                     /locus_tag="PXA33_001118"
     CDS             complement(8808..9188)
                     /locus_tag="PXA33_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HDL5120456.1"
                     /translation="MTRINLVPPTELCDQHLLAEHRELTRIPNAVAKGKFSLKGQPED
                     YKLGEGHVRFFFNKLTFLKKRYDALHEECLARGFKVQYFWAEHLPQDPSLWLDYQPTE
                     TALTVNRERIALRMPANPRFSERK"
     gene            9253..9966
                     /gene="ubiG"
                     /locus_tag="PXA33_001119"
     CDS             9253..9966
                     /gene="ubiG"
                     /locus_tag="PXA33_001119"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014667941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HDL5120457.1"
                     /translation="MQNVDQQEITKFENMAKTWWDPNGSFKPIHLLNPLRLAYILEKS
                     NGLFGKKVLDVGCGGGILSEAMAKQGAIVTGIDMTSQPLEVAKQHAKESGLEIDYQQT
                     TIENFLAKMTACNQEKFDVITCMEMLEHVPDPLSIIQSCKALLKPDGVLFFSTINRTF
                     KAYMLVIIGAEYVLKMLPKGTHEFEKFIKPAELLAWCDQADLRCQEMRGYHFNPLTEK
                     FWLNNDVSCNYIATLKPNN"
     gene            10029..10967
                     /gene="ttcA"
                     /locus_tag="PXA33_001120"
     CDS             10029..10967
                     /gene="ttcA"
                     /locus_tag="PXA33_001120"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HDL5120458.1"
                     /translation="MTDDIQKSDKKITYNFNKLQKRLRRNVGNAIADFGMIEEGDRVM
                     VCLSGGKDSYTLLDILLNLQQSAPVNFSVVAVNLDQKQPGFPEEVLPRYLESIGVEYK
                     IVEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDMLATLF
                     LNMFYGGKMKSMPPKLISDDGKQIVIRPLAYCKEKDIIKYAEAKQFPIIPCNLCGSQP
                     NLQRQVVKEMLNTWDRQYPGRLETMFSALQDIVPSHLCDPKLFDFKGIKHGQTIEGIE
                     GDTAFDDVKIEAKTFLYEDDDNNFAEAGMIQFKEVN"
     gene            complement(10951..11598)
                     /locus_tag="PXA33_001121"
     CDS             complement(10951..11598)
                     /locus_tag="PXA33_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL5120459.1"
                     /translation="MIIHILDDEESILDAMSFLLAPLGVEIQTWQSSLDFLAQADLHR
                     QGVLLLDIRMPLPDGQQVHQQLREAQSTLAVVIMTAHGDVPMAVAELKKGAVDFLQKP
                     ASFEQLKQAIAQAEKVSEKAVKIREISQNYAKLTEKERNLVPLIMQGFTNKQIADKLA
                     ISVRTVEVHRANVMEKMQAESLAGLVQMIGELQYKGSGNCSATFSFDFCRINLLL"
     gene            complement(11684..13342)
                     /locus_tag="PXA33_001122"
     CDS             complement(11684..13342)
                     /locus_tag="PXA33_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HDL5120460.1"
                     /translation="MKRFLTLFLLLFSSFSLAETHQIGILAQRGEAYTRTHWQPWVDW
                     LNSQFPNERFELVPLGINEASRRAELDFLLTNQAQFFYLSNQNVRWLATLNSPFSANG
                     EQGAVGSSIWVRADSHYQQLSDLKNQTISAVDNDAFGGFLLGLYQFHQAGMRQNRDFS
                     VQFSGFPVENALALLAEKQVEAAIVPVCLLEELEKEGKFKRSDFRLILQNPQAQGCLA
                     STPLLPNWSLAAMENVPNELAVQLATRLLNSHNPDLPRWTLPFSSAQADHILRELYRH
                     PQQKSLWATVLDWVRLNKFGLLAVVLFILLNLVALRYQVYRKSKALQQAHCKMQQYQQ
                     ELTRADRLALLGEMTTGFAHELKQPLSAVRMYAEGLKSQNSDPYQQRILDKLIAQVDR
                     AVKTMQSIRDWAQNRPSGEPQAVILNQLITNVIEFVAVENRQNAQISLIADRTFRLNL
                     HATVLEQVLTNCLLNALQAGASEISVRLQAVENGLEIAIEDNGGGFSPAQLEFPFVPF
                     RTDKPHGLGLGLVLCQRLMQSLNGRIVLANGENGAKVVLLLPQI"
     gene            complement(13342..13758)
                     /locus_tag="PXA33_001123"
     CDS             complement(13342..13758)
                     /locus_tag="PXA33_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HDL5120461.1"
                     /translation="MHSDYLVIDTNILISGLLFPKSNPNLAVKKALLGYTICVSQATF
                     DEFLEVSARPKFAKYFSIQEREEFIANLLDAVKFVEIKETITDCQDPKDNKFLEVAIS
                     AKAKYLVTGDKKDLLSMNPYRNVEIISVSEFLAETR"
     gene            complement(13748..14044)
                     /locus_tag="PXA33_001124"
     CDS             complement(13748..14044)
                     /locus_tag="PXA33_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HDL5120462.1"
                     /translation="MTAITIPQFEEVTSKQFQSEFGRIVDIVKGKTPVAITQYGRPTM
                     VLFPYDQAVEARRLLAKQRLLEMLAERDKNPPAALAEITLEELDKMIDEERNAL"
BASE COUNT         3783 a         3456 c         2985 g         4144 t
ORIGIN      
        1 attccaaata agcataacgc ataaaataat agcttgcact tgaaataaat tagagttttg
       61 tatgattggt tttcatcaca agcaaacaag cggtcaaaat tggcaatttt ttgtcaaatt
      121 taaccgcttg tattctatac tttgtttatc aatgccagca catctgcaaa taattgttta
      181 aataaagttg gaattgaatg ctctccccgt tgccccatct cattggctaa tgcaatggct
      241 aaatcaggct ttgagcttcg ttgaatcgct cgtttaatga tgtaggacac cggatatttt
      301 tcttgtgaac gccaatttgc aagctgtata aacacattcc gcaagcctgc tttgtagaaa
      361 aaatgttcga tatctttttg tggcaacatc gtgagccggt gagcaaaatc ctcttccata
      421 ctttttaccg tatctgcata acgtttgccg gaatcgtcac cgtcagtcag cacataccat
      481 tcgatcccca ttgctctggc gtatttaatt aacggacgta agccacactg agcaaattca
      541 acgattttga tcccttccat ttccaaatga atatctaata atctcgccag ctcttgcaaa
      601 atccaaacct ccgtttcccc ctcaaccaaa atccacgctt tagcaaacag tgccaagcca
      661 cgattatggt gaatatgaaa ggttaatttt cgcaaatctt ccttgcctaa actgtggcgt
      721 tcaagccaat aggcttgggt atgatcgtga tgacgcacca aacggcagat ttcacgcaga
      781 ggcacttgcg ataacaattc aatagaatta gtagtggtga tccgttggct gggtaaaaaa
      841 ccgagcagtt cccaaaaaat cgcaatcatt cgagggtgta gttgagcatt cagatcttca
      901 aataaaataa tcggcttttc tattactgta gatctaggat taaacaaaaa tagagtagaa
      961 atatgctgct gagcagattc gaccaattcc ggctgctgtt ttaggcgtaa acagagggat
     1021 tttacttttt cccaccacaa tgcggtgtct tgcatactgg ttttgatcaa atagcgactt
     1081 tggctggtta gaaaatagta tttcagtagc gaagagaggg cgtttaattc acgttgaaaa
     1141 tcgtcttcaa gttgagacga tcctttaagt aataattcat tatgcaccct gttattgagc
     1201 cttgcatcac gcaggcgata aacagggtga tggtaaataa actgccgaat ggcgttttcc
     1261 gcttgttcta tctggatcgg ttttaggctt tgatccacaa attgatagtc ggtgctaatc
     1321 tgctctccgt gtaattttcc ggttacggca agcgaaacac agcggtgatt atcaatataa
     1381 aacgcggctt gtgccaaata agcgggaaat tgcaccgcta aatcgtgctg atgctcttca
     1441 caaaaggtaa acaccagttg aatgtcccgc acttggttgg gggtatcggt gtattgaata
     1501 tggaaatctt tgaactgaaa ttgatacaac gcctgctcaa ggttaaaaat caggcttaat
     1561 gcatcaatca gactggattt cccccacgca ttttcgccaa tcaataccat atttggtcgt
     1621 agctggagcg ataaatggtt aataccacga aagccactga tttcaatatg ctttaaatac
     1681 ataccttacc ccttgttagc attacccttt tacaatttca tagcaaggtt ggtacgcctc
     1741 acccgttgga attttcatac ggtgttgaac cacaaaacgc tccaacagtt tatctatatg
     1801 ctccattaat ttacgatcgc cgtgtaattt aaacacccca tatttctcaa ttaaatcgga
     1861 ggtctccggc ttaatattac ctgcaacaat ccctgaaaat gcccgtctta aattcgcagc
     1921 cagctgctct tttggctgtg ctaaacttaa attcaggttt gccatatttt cgtgggttgg
     1981 aataaatggc gtaataaaat cttcctcaat ttttaacgac cagttgaaat tataagcatc
     2041 gccactttca taacggctgc tttttacctc aatcatcgct tgcttgactt tacgagccac
     2101 caacaccgag tcatcaataa taatttcata caaactgcga gcttcttcgc ctaaagtttc
     2161 gccaataaat tgatcgatgg catagaaata gtctgccgat tctttcgggg cggttaaaat
     2221 cagtggtaat tgctgagcat ggttagccgg gtttaactta ataccgataa tatacagcaa
     2281 ctcttccagc gttcccggcc cgcccgggaa aaccacaatg gcgtgcccta aacgcacaaa
     2341 ggcttccaac cgtttttcaa tatccggcat aattaccaat tcattcacaa tcgggtttgg
     2401 cggctcggag gcaataatag acggctcggt aatgccgata aaacgcccgt ttttataacg
     2461 ttgattggcg tgtccgatag ccgctccttt catcggggct tccatcacac cggctccgca
     2521 accggtcaca atattcagct cacgcaagcc aaactcttgc ccgacggcac ggcaatattg
     2581 gtattcggtt tcattaatcg aatgccctcc ccagcacacc accaaatttg ggtcggtatt
     2641 catttcaatc gctttcgcat tccgcaaaat cgaaaatacg gtattcgtgg ttaaggcttc
     2701 taaagaagtc tcttcaagcg gtctaatcgc attttttaac gcattcacaa acacaatatc
     2761 ccgcagcacc gcaaaaaggt gatattgaat attcaaaatc atcttgccgt caacaaacgc
     2821 agtttcgggt ggattatgca attcaagcga cacgcctttt tcacgggtta ataagcggat
     2881 ttcaaaatca gggtattggc ttaacaacgc tcggctgtca tcggtatgca caccggaatt
     2941 tagcaccgcc aaagagcaat tacgataaag ttgatgcaaa ttaccctgcg tattctttgc
     3001 caacaactcc atttccaaat gggaaagctg ttccatactc cctttcgggt tgactcgatg
     3061 tattgccatt atttactcct tgatttgcaa aaaattcatt taatatgacc gcttgttatt
     3121 ttaatagttt atcaatcacc cgactcaccg caaaaaagcc gaacgttgcc gtcaccattg
     3181 tcaccgctcc aaagccgttg gcacagttca ttgttgccga cacctcacag gcctcaccca
     3241 tttgtgggaa aatcagttgc tgagtcgaag aaacgcactc cacgccgaat ttgtttttat
     3301 tgctgaaacc gtactcttta cgcaacaggc tacgaaccct tgccgccaac ggatcttgca
     3361 ccgttttgct taaatcggta atttgaattt tggtcggatc ggtctgcccg cccgccccgc
     3421 cggtggtgat aattttgatc ttattgcgtt tacaaaacgc aatcatcgcc gcttttaccc
     3481 gcaaactgtc aatcgcatcg atcacatagt catatcctcg attgaggtag tctgcgagat
     3541 tatcctgcgt taagaaatca tcaataattt ccaccacaca ttcagggttg atccgctcaa
     3601 tccgctcttt catcgcttcg gttttcagtt gagccaccgt gccactcatt gcgtgaattt
     3661 ggcggttgat attggtcacg caaatatcgt ccatatcgat catcgtgatc ttaccgatac
     3721 ctgagcgagc caatgcctcc actgcccacg agccaacccc gccaatccca atcacgcaaa
     3781 tatgcgaacg gcgtagtttc tccaatcctt ccggcgtata aaggcgacca atgccaccaa
     3841 aacgttgttc gtagttatct aatctttctg tcattttgct tcacatttgt aggggcgaaa
     3901 aatttttcgc ccaatcattt tatccatcaa gggcgaataa tcattcgccc ctacattaga
     3961 tattcccgtt tttaatattt tccaacgctt cccaccgctc aaaggcttgc tctaacgcgg
     4021 cttcggtatc ggcaagtttt tgtagctggg cgttggtgta gcttggatct ttggcgaaga
     4081 attcggcact atttacttcg gcttgcaggg cttcaatgtc ggcttcaagc tgttccattt
     4141 tagccggtaa ctcgtccagt tcacgctgtt ctttgtagga aagtttggtt ttcttcgtcg
     4201 gctctgcttt cgttttttcc tgatcggctt tctgtttctc ttgctccgca agcggtgctt
     4261 tttcctctat tttttgcaaa tttttactcg aatttaaccg cttgtcggca gtggcggcga
     4321 agtaattttc ctgctgttgt ttggcatcga aatagccgcc gacatattta ttcaccacgc
     4381 cttcgccctc gaagaagtag cattcggtta cggtattatc cacaaattga cggtcgtggc
     4441 tcacaataat cagggtgcct tgatagtcgg tgagcatctc ttccagcagt tcgagggttt
     4501 ccacgtccaa atcgttggtc ggttcgtcca aaatcagcaa gttgttcggt ttgagtaaca
     4561 gttttgccag caacaagcag ttgcgttcac cacccgagag ggctttcacc ggtgtcatcg
     4621 cccgttttgg cgggaacagg aagtcttgca gatagccaag aacgtggcgt ttcaccccgt
     4681 ttacttctac atcttgcttg ccgtctgcca cgttgtccat cacggttttt tcagggtcaa
     4741 gatcgactcg gtattggtcg aagtacgcca cttccaattt tgtgccacaa cgcactgaac
     4801 cagatgtcgg ctgaatttcg ccaagtaaca gcttgatcag cgtggttttg ccgatcccgt
     4861 tcgctccaat cagggcaatt ttatcgccac gcaaaatggt ggtgctgaaa tttttcagca
     4921 gggttctgcc gccgatttca tagccggcat tttccacttc aaacacaatt ttgccgctgc
     4981 gtgcggtgtt gtcgatctga attttcgccg tgccttgtac ctctcggcgg tttctacgct
     5041 cttcacgcaa cgctttcaac gctcgcacac gcccttcgtt tcgggtgcgt cttgccttaa
     5101 tcccttgacg gatccagact tcttcttggg cgagtttttt gtcgaacagc tcattttgca
     5161 acgcttctac tcgcagatcc tcggcttttt cttccaagta tttatcgtaa ttgcttgggt
     5221 aggaaaccaa tttgcctcga tctaaatcca caatccgagt cgccattttg cggataaagg
     5281 cacggtcgtg cgagataaaa ataatactgc ctttgaaatc caataacaga ttttccagcc
     5341 acgcaatcgc ctcaatgtcg aggtggttgg tcggttcgtc caacagcaat acatcaggat
     5401 cggcaaccaa cgcccgagcc aatgccgcac ggcgtaccca accgcctgag agttcgcaca
     5461 ggcgtttgtc gggggcgagt tccagcagtt tcaaggtgtc ttgaatgcgg ttttcaaact
     5521 gccaaccgtt agcgtgttct aattgcgatt gcaccgctga aagtttggcg agcagttcat
     5581 cgctgtaatc ggtctgcatt tgcagggaaa tgtggtgata ttgcttcaat aagtcggcaa
     5641 gatgggcaat cccctccgcc acataatcga acaccgtttc ttcaatatgg cggggcggat
     5701 cttgttcaag acgtgtaacg acaatgtctt tttcaaatac caacttgccg tcatcaagct
     5761 gaacttcgcc cgccagaatt ttcatcaggg tcgatttacc cgccccgtta cgccccacca
     5821 aacaaacccg ctcgccgggt tcgatatgta attcagtatg atccaataaa ggatgatcgc
     5881 caaagcctaa ataggcgttg gaaagattga taagtgccat agtttttatt atctactgta
     5941 tgatccaaaa acggtcgaat ttccttctat ttttgcaaaa aattgtgaaa attcgaccgc
     6001 ttgtttagtt aagattattc ttcagttgat gtttctgctg tttcggcatt ttctaatgcg
     6061 tctaggcttt cattttcatc gtcttcaggc tcgcagactc gttcaaggct aacaacttgt
     6121 tcgtcttctg cggtgcgaat aatacgtacg ccttgggtgt ttcgtccaac gaggctgact
     6181 tctgccactc gggtgcgaac cagcgtgccg gcatcggtaa tcaacataat ttggtcggtt
     6241 tcctctactt gaactgccgc aaccactttg ccgtttcgtt cattcacttt aatcgaaacg
     6301 acccctttgg ttgctcggga tttcaccgga tattcgctaa tcaccgtacg tttaccgtag
     6361 ccgttttggg tgacggttaa aatatcgcct tcggtgcgag gaatcactaa agaaaccaca
     6421 cggtctttat caaggctgac gctggaggaa tcttctgcct cttcattgtc agtttcaata
     6481 atttcagctt cttcaatttc ttcgcttgaa atttcggtgg tagaaagttt aatacctcgt
     6541 acgccggtgg cggtacgtcc catcgcacga actgctgttt cggcaaagcg aactactcgc
     6601 ccttgtgccg agaacaacat aatttcgctg ttaccatcag taatatctac cccgatcagc
     6661 tcatcgcctt cacgaagttt taacgcaatc agaccgcttg cacgcacgtt gctaaaggca
     6721 tcaagggaaa cttttttcac tacaccgcta gctgttgcca taaagataaa cttatcggca
     6781 ctaaattcac cgtttggaat cggtagaatc gcggtaatac gttcgttttc ttctttcact
     6841 aacggcagaa tattcacaat cggcgtgcct cttgccccac ggcttgcttg cggtaattga
     6901 tacactttta attgatataa acgcccacgg cttgagaagc aaagaatggt gtcgtgggtg
     6961 tttgccacca agagtttttc gatgaaatca tcttctttca tcttggttgc cgacttacct
     7021 ttaccgccac ggcgttgtgc ttcgtagtcg gataacggct gatatttcac atagccggcg
     7081 tgagaaagcg taaccaccac atcttcttgt gcgattaagt cttcaatgtt gatgtcgcca
     7141 gaggctgcgg taatttcagt acggcgttca tcattaaaat cggtttttac acgttctaac
     7201 tcttcacgga tcacttccat taagcgttct gggctggtta aaatgtggat taattcgcca
     7261 atttcagtta agagttcttt gtattcttca acgattttct cgtgttctaa accggttaag
     7321 cggtgtaagc gtagctctaa aatcgctctg gcttgagctt cagataaata gtattcgcca
     7381 tcacgcactc ctaagtgttc aggtaaatct tccggtcggg aagcaccaac accagcagcc
     7441 tctaacattg gtgcaacatt gcctaatgcc caagggcgag ccaataagcc ttcacgggct
     7501 tcttctgcgg tttttgaggc tcgaattaat tcaattaccg ggtcgatatt cgctaacgca
     7561 atcgccaaac cttctaaaat atgggcacgt tcacgggctt tacgcaattc aaaaatcgta
     7621 cggcgtgtga ccacttcacg acggtgttta acaaaggctt cgatgatctg gcgtaagttc
     7681 agcacttttg gctgcccatt atctaacgcc accatattga taccaaacgt gacctgcatt
     7741 tgggttaaag aataaaggtg gtttaacacc acttcaccca ctgcatcacg cttgacttca
     7801 ataacaatcg aaatcccttt tttatcggaa tagtcatcaa ttttgctgat gccttcgatt
     7861 ttcttctctt taaccagctc ggcaattttt tcaattaatt ttgccttatt cacttggtat
     7921 ggcagttcgg ttacaacaat ttgctcacgc cctttatcag ttgtttccac gctggctttc
     7981 gcgcgcacat acaccttgcc acgaccggtg cggtaagcct cttcaatccc tttacgaccg
     8041 ttgataatcg ccccggtcgg gaaatccggc cccgggatat aacgcattag ctcttcaaca
     8101 ctaatctgtt cattttcaat atacgccaag caaccatcta atacttcgcc taaattatga
     8161 ggtggaatat tggttgccat accgaccgca atccctgaag aaccattgac taacagagcc
     8221 ggaattttgg ttggcaatac atcaggaatc atctctttgc cgtcatagtt cggtgagaaa
     8281 tccaccgttt ctttatcgag atcggttaat aattcctgcg taattttctg catacgcact
     8341 tcggtataac gcattgctgc cggtgcatcg ccatcaatag aaccaaagtt accttgcccg
     8401 tccaccaaca tataacgtag tgagaacggt tgagccatac gcacaatagt gtcatacacc
     8461 gcagagtcac cgtgtgggtg atatttaccg atcacatcac cgaccacacg ggcagattta
     8521 acgtgcggct tattataggt gttgctgttt tgatccattg aaaaaagcac tcggcggtgt
     8581 accggcttta aaccgtctcg tacatcaggc aaggcacgcc caacaatcac cgacatcgca
     8641 tagtcaaggt aagaggtttt taattcgtcc tcaatactta cgggaataat atctttggct
     8701 aattcgctca ttgaaatttc ctaattttat tagtaaaaga gccttaaata aggcgaaaaa
     8761 attggcgaga ttatagcata aaaagcaaca aaaaggcgta caagcggtca ttttctttcg
     8821 ctaaaacgtg gatttgccgg cattcttaat gcaattcgct cccgatttac agtcagagcg
     8881 gtttcagtcg gctgataatc tagccagaga gagggatctt gtggtaaatg ttccgcccag
     8941 aaatattgca ccttaaaacc tcttgctaaa cattcctcgt gtaacgcatc gtaacgcttt
     9001 ttcaaaaacg tgagtttatt gaagaaaaac cgaacgtgtc cctcgcccag tttgtaatct
     9061 tctggctgtc cttttaggct aaatttgcct ttcgctaccg cattcggaat ccgagtaagt
     9121 tcacgatgtt cggcaagcag atgttgatcg cataattccg ttggcggaac aaggttaatt
     9181 cgggtcattc tgattttcct tttaggctaa atgcgttata gtaatgccct aaatttacca
     9241 taggaacagt ttatgcaaaa cgtcgatcag caagaaatca caaaatttga aaatatggct
     9301 aaaacctggt gggatccaaa cggcagtttc aagccgattc atctgcttaa tccactgcgt
     9361 ttagcctata ttttagaaaa atcgaacggt ttatttggca aaaaggtgtt agatgtcggc
     9421 tgtggtggcg gtattttaag tgaggcaatg gcaaaacagg gagcgattgt taccggcatc
     9481 gatatgacct ctcaaccgct tgaagtggca aagcagcacg ccaaagaaag tgggttagag
     9541 attgattacc aacaaaccac gattgaaaat tttttagcaa aaatgaccgc ttgtaatcaa
     9601 gaaaaattcg atgtgattac ttgtatggaa atgctggaac acgttcccga tccgctttct
     9661 atcattcaaa gctgtaaagc cctgctcaaa cctgacggcg tgctgttttt ctctaccatc
     9721 aaccgtactt ttaaagccta tatgttggta attatcgggg cggaatatgt gctgaaaatg
     9781 ttgccaaaag gcacgcacga atttgaaaaa ttcatcaagc ctgccgaatt gttagcttgg
     9841 tgcgatcagg ctgatttacg ttgccaagag atgagaggct accatttcaa cccattaacc
     9901 gaaaaatttt ggctcaataa tgatgtgagt tgtaactata tcgctacgct gaagccgaat
     9961 aattaacttg caaaatagtg cgaaatcttt actatacacc gctaatttct aagcctagta
    10021 caactattat gacagacgat attcaaaaat ctgataaaaa aattacttat aatttcaaca
    10081 agttacaaaa acgtttacgc cgtaatgtcg gtaatgccat tgccgatttt ggtatgattg
    10141 aagagggcga ccgtgtaatg gtctgccttt ccggcggtaa agacagctat acgctgttag
    10201 atattttgct caatttacag caaagtgcac cggtgaattt tagtgtggta gcggtcaatt
    10261 tagaccaaaa gcaaccggga tttccggaag aggtattgcc tcgctattta gaaagcattg
    10321 gcgtggaata caaaattgtg gaagaaaaca cctacggcat cgtaaaagag aaaattccgg
    10381 agggcaaaac cacctgttca ctttgctcac gacttcgccg tgggatttta taccgcaccg
    10441 ccaccgaatt gggtgcaacc aaaattgcat tagggcatca ccgtgacgat atgctggcga
    10501 ctttattctt aaatatgttc tacggcggca aaatgaaaag tatgccacct aaactgatct
    10561 ctgatgacgg caagcaaatt gtgatccgcc cactcgccta ttgtaaagaa aaagacatta
    10621 tcaaatatgc cgaagccaag cagttcccga ttattccgtg taatctgtgt ggttctcagc
    10681 ctaatttaca acgccaagtg gtgaaagaaa tgctgaatac ttgggatcgc caatacccgg
    10741 ggcgtttaga aacaatgttc agtgctttgc aagatatcgt gccatcccac ttatgcgatc
    10801 cgaaattgtt tgattttaaa gggattaaac acggtcaaac cattgaaggc atagaaggcg
    10861 acaccgcttt tgatgatgtc aaaattgaag ccaaaacctt cttatacgaa gatgacgata
    10921 acaattttgc cgaagcggga atgattcagt ttaaagaagt aaattaatac ggcaaaagtc
    10981 gaaactaaag gtagcagagc aatttccgct acctttatat tgtaactccc cgatcatctg
    11041 caccagcccc gccaaactct ccgcctgcat tttttccatc acattggctc gatgtacttc
    11101 caccgtacgc actgaaatcg ctaatttatc tgcgatctgt ttattggtga aaccttgcat
    11161 aatcagtggc acaaggttac gctctttttc cgtcaatttg gcgtaatttt gcgaaatttc
    11221 acgaattttc accgcttttt cacttacttt ttccgcctgt gcaatcgcct gttttagctg
    11281 ttcaaaactt gccggctttt gcagaaaatc cactgcgcct tttttcaatt cagcgactgc
    11341 catcggcaca tcaccgtggg cggtcataat caccaccgcc agggtcgatt gtgcctctcg
    11401 cagttgttga tgaacctgtt gcccgtctgg cagcggcatt cgaatatcta gtaacagcac
    11461 gccttgccgg tgtaaatccg cctgtgccaa aaaatctagg ctggattgcc acgtttggat
    11521 ttccacccca agcggggcaa gcaggaacga catagcgtct aaaatgcttt cttcgtcatc
    11581 aagaatgtga ataatcattt tttctttttt ccgtaggggc gggtcctgtg cccgccctga
    11641 atttgatagg atttacgggc aggcacagga cctgccccta catttatatt tgagggagta
    11701 aaagcactac cttcgccccg ttctccccgt tcgccaatac aatccgtccg ttgagcgatt
    11761 gcatcagccg ttggcatagc accaagccta gccctaagcc gtgtggtttg tcggtgcgaa
    11821 atggcacaaa cggaaattca agctgggcag gggaaaaacc gccgccgttg tcttcgattg
    11881 caatttccag cccgttttcg accgcttgta accgcaccga tatctcgctt gcaccggctt
    11941 gcagggcgtt gagcaggcag ttcgtcagta cttgttccag taccgtggcg tgtaaattaa
    12001 gccgaaaagt gcggtcggca atcagcgaga tttgtgcatt ctggcgattt tccaccgcca
    12061 caaactcgat cacattagta atcaattggt tcaaaatgac cgcttgcggt tcaccactcg
    12121 ggcggttttg cgcccagtcc cgaatgctct gcatcgtttt cactgcccga tctacctgcg
    12181 caatcagctt atccaaaatc cgctgttgat aaggatcgct gttttggctt ttcagcccct
    12241 cggcgtacat tcgcaccgcc gacaacggct gtttcagttc gtgagcaaaa ccggttgtca
    12301 tctcgcccaa caaagcaagg cggtcggctc gggtcagttc ctgctgatac tgctgcattt
    12361 tgcagtgtgc ctgctgtaac gccttgcttt tgcgatagac ctgataacgc agggcgacaa
    12421 ggttgagcaa aatgaacaac accaccgcca acaagccgaa tttattgaga cgaacccaat
    12481 ccagcaccgt tgcccacagg cttttttgtt gcgggtggcg atacagttca cgcagaatgt
    12541 gatcggcttg ggcggaagaa aacggcaacg tccagcgggg taggtcggga ttgtggctgt
    12601 ttagcaagcg tgtggcaagc tgtaccgcca gctcgtttgg cacattttcc attgccgcca
    12661 gcgaccagtt cggcaacaac ggcgtgctgg caaggcaacc ctgcgcctgt gggttttgca
    12721 aaattaagcg aaaatccgac cgcttgaact tgccctcttt ctccaactct tccagcaaac
    12781 agaccggtac aatcgccgct tccacctgtt tttccgccag caaagccaac gcattttcca
    12841 ccggaaaacc gctaaactgc accgaaaaat cccgattttg ccgcattcct gcttgatgaa
    12901 actgatacaa cccaagcaaa aaaccgccga aagcatcgtt atccaccgca ctgatagtct
    12961 gatttttcag atcgcttaac tgctgataat ggctgtcggc acgcacccaa atgctactgc
    13021 ccaccgcccc ttgctcacca tttgccgaaa acggactgtt gagcgtggca agccatcgca
    13081 cattttggtt actcaaatag aaaaactgag cttgattggt cagcagaaaa tccaactccg
    13141 cccggcggct cgcctcgtta atccccaatg gaaccagctc aaaccgctcg ttgggaaact
    13201 gcgaatttaa ccaatccacc caaggctgcc aatgggtgcg ggtgtaggct tccccacgct
    13261 gagcgagaat gccgatttgg tgggtttcgg ctaatgaaaa agatgaaaaa aggagaagaa
    13321 aaagagtgag aaaccgtttc attagcgagt ttccgctaaa aattcgctga cggaaataat
    13381 ttcaacattc cgataagggt tcatactgag gagatctttt ttatcgcccg tcactaaata
    13441 cttcgctttt gcactgatcg ccacttctaa aaatttatta tctttgggat cttggcaatc
    13501 tgtaatcgtt tctttaattt ccacaaactt tactgcatcc aacaaattgg caataaattc
    13561 ttccctttcc tgaatactga aatatttagc aaatttcggt ctagcggaga cttctaaaaa
    13621 ttcgtcaaaa gtcgcttgcg agacacaaat ggtatagcct aataaggctt ttttcactgc
    13681 taaattggga tttgattttg ggaacaataa accactaatc aaaatattcg tatcaatcac
    13741 taaataatca gagtgcatta cgttcctcat caatcatttt atccaactct tcaagcgtga
    13801 tttctgcaag agctgccggc ggattcttat ctcgctctgc aagcatttca agcaaacgct
    13861 gttttgctaa taatcgccga gcttcaaccg cttgatcgta aggaaaaagc accattgtcg
    13921 gtctgccata ttgggtaatc gccacagggg ttttgccttt tacgatatca acaattctgc
    13981 cgaactcaga ttgaaattgc ttagaagtca cctcttcaaa ttgagggata gtaatcgctg
    14041 tcatttttaa gctcctaatg tgagtgattt tggatatgtt agctatgttt tgtgtaaaaa
    14101 tcaatcgagc caattaagat aaatttttgt agttattgta aattcaataa cataaaaact
    14161 tgaacaaaca cttgattttc tattttttat ggcttaatgt agtagagcat attatatgta
    14221 ggttctatct ccaggcctgc ggtcaggtat ccatacttgc cttgaacaaa agagataatt
    14281 cgatagatta tattgcttat agtcaattca tttataaatt aaatcctttt atttcctagt
    14341 atggctgtga ttttcacaga ggcatttt
//
LOCUS       DANLRK010000053        14137 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.053, whole genome shotgun sequence.
ACCESSION   DANLRK010000053 DANLRK010000000
VERSION     DANLRK010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 14137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14137
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.053"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            <1..2193
                     /gene="traD"
                     /locus_tag="PXA33_001125"
     CDS             <1..2193
                     /gene="traD"
                     /locus_tag="PXA33_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HDL5120463.1"
                     /translation="HTLEAWLRPPVEFFPAIVHASCAFVSAAAPWSLALHPSIGYSIA
                     AGFGALSFYRAKQGWQIVRYHQNLKRLPNYSMTSKQIPVSNKYLFVGKGFEWKPIHTQ
                     RLHDCFSDNGSKYLEPSKLFKMAREYEKRHENWLTRLTSKDSRWNPVRPLPPVEGIPA
                     IHGIEPDEVDVVQSVSSRVGHTVVFGTTRVGKTRLAEVFVTQDIHRGKTPDEREVVVF
                     FDPKGDADMLKRMYAEAKRAGREDEFYVFHLGYPEISARYNPVGRFNRISEVAGRISG
                     QLSGAGNSAAFKEFAWRFVNIVARALVELGNRPNYVQISQFVQNIDSLFLDYAEKFIR
                     KTDPKIWENLATIAAGLDEKALPFGMKDRPLIFVINKYIAESAVFDPILQGLASAVRY
                     DRTYFDKIVASLLPLLEKLTTGKISELLSPDYADILDERPIFDWESIIRKRGIVYIGL
                     DALSDATVAAAVGNSMFADLVSMAGHLYKFGTNEGLPGDMGRKTKLVKINLHCDEFNE
                     LMGDEFIPLINKGGGAGVQVTAYTQTLSDIEARIGSKPKAGQTLGNFNTLYMLRVKEP
                     ATAELLTKQLHEVTIKRSTVTSSTTDSSNPDDDKAFSSNTGQRISESTVPMLVPANIT
                     NLPKGQAFALLDGSKLWKIRMPLPAVDKDDMMPQSLRELTDYMRKNYHVVENWWQPAF
                     TEYTPSRDIAEAFEAMSEPQYNSRIDNQVDNFGIDEPYDIDEEENDDE"
     gene            2186..2539
                     /locus_tag="PXA33_001126"
     CDS             2186..2539
                     /locus_tag="PXA33_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemophilus-specific protein"
                     /protein_id="HDL5120464.1"
                     /translation="MSSTKMREENRTNLLDLPNKYRNFDGEFSASCGLDNAEELLIHS
                     QSYFIEWFQQGYSFHQFAEKFAHQGLSLWSADEISMRNTDKSKDVFAFYLAFDHNPSG
                     YILVQCQLDREDSLQ"
     gene            2536..3231
                     /locus_tag="PXA33_001127"
     CDS             2536..3231
                     /locus_tag="PXA33_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03747 family integrating conjugative element
                     membrane protein"
                     /protein_id="HDL5120465.1"
                     /translation="MSEGAKPRRKSGLISLILGKINMLLAAITVSLIFSIILEWVGIA
                     FWWPEEGHLHSQTMMLNEMQWLSDDFSQSLFAKSSTAFATLIIENVYNWLFVYTGIKE
                     WLTSPGNGSMEQWAYHYGRAYIESVIYVVITFIIRLIIIIFTSPLFLLTALSGLTEGL
                     SLRDIRKFGAGRESAYLYHHARRYIMPMMVTAWVVYLSIPFSIHPNFILVPAACLFGL
                     SICITAASFKKFL"
     gene            complement(3261..3620)
                     /locus_tag="PXA33_001128"
     CDS             complement(3261..3620)
                     /locus_tag="PXA33_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5120466.1"
                     /translation="MRYLALFPVFLPSIAYPLPQAHKVGDICHAENAPYNYGGLWELS
                     LRGNLVCNYWQFLGDDERKRIESQLDEYGELPLWYSSERETEEDEYPQYWEVEDWDGD
                     DWDIETENEGTNTITDE"
     gene            3818..4144
                     /locus_tag="PXA33_001129"
     CDS             3818..4144
                     /locus_tag="PXA33_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RAQPRD family integrative conjugative element
                     protein"
                     /protein_id="HDL5120467.1"
                     /translation="MSISTSLRKGIVVLVTLSCLPAFASTEQEQLAQAIKQLEAAKLS
                     LQRAEKVAKASPKTREYFNYSAIHRDIETVKKGIQQYINPSRALPRDPNVLRTLSEDY
                     SKLRGQ"
     gene            4144..4377
                     /locus_tag="PXA33_001130"
     CDS             4144..4377
                     /locus_tag="PXA33_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03758 family integrating conjugative element
                     protein"
                     /protein_id="HDL5120468.1"
                     /translation="MSYSPVDAFSAASGIDPFRLKLMIAIIFLSMLLLAVAWALNSGY
                     KGFSFRDKSPFSLLVLFLKGVALLVCVTYFFIY"
     gene            4392..4781
                     /locus_tag="PXA33_001131"
     CDS             4392..4781
                     /locus_tag="PXA33_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2976 domain-containing protein"
                     /protein_id="HDL5120469.1"
                     /translation="MSRIKSLTKHLHSRYLAVMLVFWGTIQHAFAAGPDASKIPDFQI
                     PGVDSNTKDPMEILWIVGKSIITLFAVLLAAGAIFVVVKALISIYNEATSENGKKGWG
                     HFIVALIIGFIVIFFCLWLVKLAVGLF"
     gene            4804..5169
                     /locus_tag="PXA33_001132"
     CDS             4804..5169
                     /locus_tag="PXA33_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03750 family conjugal transfer protein"
                     /protein_id="HDL5120470.1"
                     /translation="MPEQDTTIPFLPTRLNREATVYGGMTVSEFGISAGLGFMLGLIV
                     GLFLWILTDFWLLIPAMAMLLCIATVLIGKGVVAAVKRGKPEAYLNRLIEKKMDDLFN
                     GHKFIRREGFWSIRRYKRK"
     gene            5173..5817
                     /locus_tag="PXA33_001133"
     CDS             5173..5817
                     /locus_tag="PXA33_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03746 family integrating conjugative element
                     protein"
                     /protein_id="HDL5120471.1"
                     /translation="MSQLKSQLHEKDKHIKTTRIFAFLFFIIILVLAGVIYTFPSRLT
                     IYNPPDLRAGSQRAWWEIPKPTIYAFSYQIFQQMNRWMTNGEEDYEKNITALKPFLTP
                     HCEAFLRQDYKDRLRNNELRSRTRMVYEITGRGYSEDKVTVFSEDNWAVNLDLSVDEH
                     FQGQPVKRVYIRFPISIVRMDIDSVKNPWGLGFNCYHSIPKLLEGVEPKTEEKK"
     gene            5817..6698
                     /locus_tag="PXA33_001134"
     CDS             5817..6698
                     /locus_tag="PXA33_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03749 family integrating conjugative element
                     protein"
                     /protein_id="HDL5120472.1"
                     /translation="MKRLPILFASLLISFSGSSFADVLMKWERKPLPIALQVEKERII
                     FVDKNVKVGYPAELDGKLRIQSTGGAVYLKALETFPSTRIEFRDVSTGEIFLFDIVSA
                     TKVANPEESIRMVFDSAVEKQSFSTNRDDEPDLSLTGTRPQLPIPAALTRYAAQSLYA
                     PLRAVEPLEGVRRVAHRLPKKITTLLPAYSIEATPLISWQLDEYVVTAIRLQNKGNSQ
                     ITLDPRELQGQFYAATFQHNWLGGYGGNEDTTTLYLVTEGYANNALIPETHPVKPVGK
                     KVKKTTKVETKPTQSAK"
     gene            6710..8164
                     /locus_tag="PXA33_001135"
     CDS             6710..8164
                     /locus_tag="PXA33_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03752 family integrating conjugative element
                     protein"
                     /protein_id="HDL5120473.1"
                     /translation="MKANKALFVILGIVFTLVGGIVYALFWRSDTPQEPTEQEILDIS
                     IKDLTPEQVRSMGIQGDTPIDTLRTLVTTVKSNERRMDELIDENRQLKQKEDLVNGRI
                     SDAVNNETRKLQEQIADLKMQLFDMATHVNNKSTAQESTPQAEAQPSMTTNKGFGTAG
                     DKLPIGGASMPNVDIGGMYWVEPSDQIGLDAGGKAVSGNTLATKFGFPNDFKGGQTTG
                     NTEKKGLPFFPETDVVTGSQGTTGNSRGKNQITPVYTVPSNSTFLDSVAMTGLIGRIP
                     IDNKVSDAYPFKVLIGRENLIANGIELLDVEGAIVSGTATGDWTLSCVRGDVKSMTFV
                     FNDGRIVSTTNKGNSENIGWLSDSSGIPCVPGERKTNAPEYLGTNFFLAAASAAAQGF
                     SQSQTTTVVDGNSVVGAVTGDQGKFILGQAIGGGLKESADWFRKRFAENFDAVYVPPG
                     HRVAIHIEKEIEIDYNSKGRKVKYGNTSTNTQLD"
     gene            8177..8575
                     /locus_tag="PXA33_001136"
     CDS             8177..8575
                     /locus_tag="PXA33_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03751 family conjugal transfer lipoprotein"
                     /protein_id="HDL5120474.1"
                     /translation="MKALYCIMMSALLITGCTTSTEELLPANGSTMRDIWDKGAGNND
                     LHLYRSQQGRTLDPVDYIPNTEQRAYTRTAENEINNLFPRLPNPDLIMYVYPHQTRSG
                     EPLSIPGYSTVLPFYGRVQYAQPGDRTRGL"
     gene            8575..11412
                     /locus_tag="PXA33_001137"
     CDS             8575..11412
                     /locus_tag="PXA33_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer ATPase"
                     /protein_id="HDL5120475.1"
                     /translation="MMSFLSEVFSSFKPTKTEVISSEPNENSGESDIIQNKMSEVNGE
                     VQPLKRSGKLTLSQHHRRLYSQAPSFIDHLPWGEYLNQHGVILLDDARSVGAVFDIKP
                     IGTAGRSIEFLSRIRNLVKDALQDSFDELDINPYVVQFYCQDSDDLQPYIDQLRNYIS
                     PIAQNSAFSEEWLRVQAKHFKDIGKPEGLFIDDRVTNTAWSGRIRSTKMVIYRYVDKS
                     NDSNENLIIEQLNNACSRINGALTTAGLQLQRLDGEQIRQWLLRWFNPNPSINFEGLS
                     NNDCYHLFGKQTSNDDLPLAFTDFSEDLFYSQPRNEGSYWYFDGQPHEVVVVNNLRKQ
                     PEVGLMTGEITRGRNINALFDLLPESTIMAITIVIHPQDKLEAHLEKISSRAVGENFE
                     SKYLKEDCQTVQAYLKEGHKLYQAAIAFYVRGKDEKELRTRSRQLRTILLSNNLEPVK
                     ENAEIAPLNSYLRWLPMNFNPQMDAKSHYYTKYYFVQHLANLLPIFGRDTGTGNPGIT
                     YFNRGGAPFNFDPFNRKDRTKNAHKLLLGPTGSGKSATLCGQLCQAMAVYRPRTFVVE
                     AGNSFGLLADYAERFGLTVNRITLAPNSGIVLPLFSEAHRLLDMELNPEDVPEDIEDN
                     DDTDDEGDMQRDLLAEMELTARLMITGGEKKEDEKMSRADRAAIRRAILNAARQTKSE
                     GRQTLTEDVKKALEAISRDENTREERRTRLAEMAESLEMFCTGVNGQFFNREGEIWPE
                     ADLTLVDLAMFAREGYEAELAISYISLINHINSLGEKYQHSDRMILNVTDESHIITAN
                     PLLAKFLVKGSKMWRKLGIWLWLATQNMDDFPNEAAKLLSMVEWWELLNVSASEIDEI
                     NRFRKLSEAQKTMLLSAKKSDRKYTEGVVLATNMEALFRVVPPSLFLALGMTEKHEKA
                     ERRQLMMQHGYSELDAALEVAARIDKARGIA"
     gene            11412..11840
                     /locus_tag="PXA33_001138"
     CDS             11412..11840
                     /locus_tag="PXA33_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5120476.1"
                     /translation="MMKQHYRLFNVGPQKSSLGIVSSAVETQLLGFCEQIRQLIGIGK
                     QITLFPHRVVQNHWAIELSGEKGSVHLLLNVTGRSLLPTSNYREWPARLVIEIADHVD
                     AYWFATFLAQQLDLKSNLFFLSTSIVDNSMHNEVTDTNEA"
     gene            11830..12147
                     /locus_tag="PXA33_001139"
     CDS             11830..12147
                     /locus_tag="PXA33_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemophilus-specific protein"
                     /protein_id="HDL5120477.1"
                     /translation="MKPDTTMPKMKKVRAVLKTTLIIIGIILLLLIVGWLAMTYFPTT
                     NFQAWFYETRYAWLGWRFVLYGVIWFLMVKLNRRGQMPLKARLMILSAIPLIEGINLL
                     YLL"
     gene            12158..13675
                     /locus_tag="PXA33_001140"
     CDS             12158..13675
                     /locus_tag="PXA33_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG N-terminal
                     domain-containing protein"
                     /protein_id="HDL5120478.1"
                     /translation="MTFTVDSYLEYFLTLLAWIINNNIFAVLVQTGLFILPLIFVLIG
                     TWMEVKKQGEDEGNKGDLLITWLSLKFYPAMFVVVLVLAPMIPINLNNIELNVERSKA
                     CGYRIPTSPEDSGYNQMNSELGNQQARVPLWWGLFHQINKGVTHALTASIPCKPDLRQ
                     VRFEVQHEKINNPALLTEVRLFVQQCYVPARQKIQSSQLSLTPAQVRETSWLGGRILL
                     TNSELYPRYRAQQPHSLFPYNASRDNGLPNNGEGGFPNCAEWWDTANTGLKDRLLADV
                     KQNFSAKVIDFFSSKENADESILRTVLRAENMDISRGKVYPGYGGNLDPTFANATTRL
                     TSSVGSLAASFAAFPAFDAMRQALPMVHAFMLMAVIIITPLVIVMSGYSLKTLVTLTF
                     VSFALVSLTFWWELARWLDSWLLSALYDSSAHSRINPYFLENTQDDFIVNFVMGSMFL
                     VLPAIWLGSMSWAGVKIGDIAAGFTTDSLDSKSAGSQGGNLVKKGGKEISAGFNK"
     gene            complement(13728..14108)
                     /locus_tag="PXA33_001141"
     CDS             complement(13728..14108)
                     /locus_tag="PXA33_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5120479.1"
                     /translation="MKFIDFGKQLAKPVKFIKQRTAFLPKPLQQAVVFAVIFAGLPLF
                     LVGFTVLRILNGTPVEDISTNDVAYNPNHVENTVNEIEDDSEDSKPEDLWDVIADQDE
                     NQLDGYRDGYFGYGLYQGGYFVKR"
BASE COUNT         4213 a         2733 c         3025 g         4166 t
ORIGIN      
        1 cataccttgg aggcgtggtt acgccctcct gttgagtttt ttccggcaat tgttcatgct
       61 tcttgtgctt ttgtcagtgc tgctgcgcct tggtctctag cactccatcc aagtatcggc
      121 tatagtattg cagccggttt cggtgcgttg agtttttatc gagcaaaaca aggatggcaa
      181 attgttcgtt accatcaaaa tctgaaacgt ttaccgaatt attcaatgac gagtaaacaa
      241 attccggtca gtaataagta tctatttgtc ggcaagggat ttgaatggaa acctattcac
      301 actcaacggc tacacgattg tttttcggat aacggttcaa aatatctgga gccttctaag
      361 ctatttaaaa tggctcgtga gtatgaaaaa cgccacgaaa attggctaac acgtcttacc
      421 tcaaaggata gccggtggaa tccggttaga cctctgccac cggttgaggg tattcctgct
      481 atccatggaa ttgagccgga tgaagttgat gtcgttcaat ccgttagctc tcgggttggg
      541 catacggtag tattcggtac aacacgtgtc ggtaaaactc gtttagctga ggtctttgtt
      601 acccaggata tccaccgagg caaaacaccg gatgagcgtg aggttgttgt ctttttcgat
      661 ccgaaaggcg atgccgatat gctcaaacga atgtatgcag aagcaaagcg agctggaaga
      721 gaagatgagt tttatgtttt ccatttggga tatcctgaaa tttcggctcg ctataaccca
      781 gtgggacgtt ttaatcgtat atcagaagtc gctggacgta tttcaggaca actcagtgga
      841 gccggtaact cagcggcatt taaagagttt gcttggcgat ttgtcaatat agttgcacgt
      901 gcattagttg aattaggaaa tcgtccaaac tatgttcaga tttcccaatt tgtacaaaac
      961 attgattcat tattccttga ttatgcggaa aaatttattc gtaaaactga ccctaaaatt
     1021 tgggaaaatt tagcgacaat agccgcagga ttagatgaga aagctcttcc ttttggaatg
     1081 aaagatcgcc ctctaatttt tgtgatcaac aaatatatcg ctgaaagtgc agtgtttgac
     1141 ccgattttac aaggactcgc cagtgccgtg cggtatgacc gtacctattt tgataaaatt
     1201 gttgcttcgt tattgccgtt attggagaaa ttaacgaccg gaaaaatttc cgaactttta
     1261 tctccggatt atgccgatat tttggatgaa cgccctattt ttgactggga atccattatc
     1321 cgtaaacgag gaattgttta tattggtttg gatgctctct ccgatgctac agttgctgcc
     1381 gcagtcggaa acagtatgtt tgcggattta gtatcaatgg caggtcattt gtataaattt
     1441 gggacaaatg agggattgcc gggtgacatg ggcagaaaaa ccaaactggt gaaaatcaat
     1501 cttcattgtg atgaattcaa tgaattgatg ggcgatgaat ttatccctct catcaacaaa
     1561 ggtggtggtg ccggagtaca agttactgct tatacgcaaa cgctctccga tattgaagcc
     1621 cgtattggca gtaaaccgaa agcgggacaa accttaggta actttaatac cctatatatg
     1681 ctcagggtaa aagaacccgc tacggcagaa ttgcttacaa aacagttaca cgaagtgacg
     1741 attaagcgta gcacggtcac gtccagtacg actgactcca gtaacccgga tgatgacaag
     1801 gcatttagct caaatacagg gcagcggatt agtgaaagta ctgttcctat gctggttcct
     1861 gccaacatta ccaatttacc aaaaggacaa gcattcgctt tactggatgg tagtaaatta
     1921 tggaaaattc ggatgccgtt accggctgtc gataaagatg atatgatgcc tcaatctcta
     1981 cgagagctga cagattatat gcgtaaaaac tatcacgttg tcgaaaattg gtggcaacca
     2041 gcatttacag aatatacgcc gtcccgagat attgcagaag cttttgaagc gatgtcagag
     2101 cctcaatata actcacggat agacaatcag gtcgataatt tcggcataga tgaaccctat
     2161 gatattgatg aagaggaaaa tgacgatgag tagtaccaaa atgagagaag aaaatagaac
     2221 caacctctta gatttaccca ataaataccg taattttgat ggcgagtttt cagcttcttg
     2281 tggactagac aatgccgaag agctccttat tcatagccaa tcctatttta tcgagtggtt
     2341 tcaacaaggc tactctttcc atcaatttgc tgaaaaattt gcacatcaag gactttcatt
     2401 atggtctgct gatgaaatat ctatgcgtaa tactgataaa tctaaagatg ttttcgcttt
     2461 ttatcttgca tttgatcata atccttctgg ctatattctt gttcaatgtc agctcgatcg
     2521 ggaggatagt ctgcaatgag tgaaggagct aaacctcgtc gaaaatcagg tttaatcagc
     2581 ctgattttgg gaaaaataaa tatgttgctt gctgcaatta cagtatctct catattcagc
     2641 attattttag agtgggtagg aattgcgttt tggtggccgg aagaaggaca cttgcatagc
     2701 caaacgatga tgctcaatga gatgcaatgg ctatcagatg atttctctca aagcctgttt
     2761 gccaaatctt ctactgcttt tgcaacattg attatcgaaa atgtctataa ttggttattt
     2821 gtttatacgg gtattaagga gtggctaact tcaccgggta acggtagtat ggaacagtgg
     2881 gcatatcact atggtagagc ctatattgag tctgttattt atgtcgtgat cacctttatt
     2941 attcgcctca ttattattat ttttaccagt cccctctttc tattgaccgc actttctggt
     3001 ttaaccgaag gattatcatt acgggatatt cgaaaatttg gtgccgggcg ggaatcagcc
     3061 tacctatacc accacgctag acgttatatt atgccgatga tggtaacagc ttgggtagtt
     3121 tatttgtcta tcccgttttc gattcaccct aacttcattt tagtccctgc tgcctgttta
     3181 tttggtttgt ccatctgtat aactgcagcg agcttcaaga aattcttgta aagataataa
     3241 gcctcagtaa tatactgggg ctattcgtct gttatagtgt ttgtcccttc attttctgtt
     3301 tcaatatccc aatcatctcc atcccaatct tcgacttccc agtattgtgg atattcatcc
     3361 tcttcggtct ctcgttcaga gctataccaa agaggcaatt ccccatattc atccaattgt
     3421 gactctatgc gttttctctc gtcatcaccc agaaattgcc aataattaca gactaaattt
     3481 cccctcaaac ttaattccca aagaccaccg taattatatg gggcattttc tgcatggcag
     3541 atatctccca ctttatgtgc ctgcggaaga gggtaagcaa ttgatgggag aaatacaggg
     3601 aatagagcta aatatctcat ctggtatctc atggtaaagc gtgaatactc aactttaata
     3661 ctaacttatt aagtaacact gacacagttg aatatctttc taaccattta atattttgtg
     3721 caatttctct tctgattggt aacagagttt tccattaaat tttaggatcg cttttggcac
     3781 tcttaacttg aagtaaaact aaaaatagag gtgcactatg tctatttcaa catctttaag
     3841 gaaaggtatt gttgttcttg ttaccttatc ttgcttgccg gcatttgctt caacagaaca
     3901 agagcaatta gctcaggcaa ttaagcaact ggaagcggct aaattatcac tccaacgagc
     3961 agaaaaagtg gctaaagcct cgcctaaaac acgagaatac ttcaactatt ctgccattca
     4021 ccgtgatatt gaaaccgtca aaaagggaat tcaacaatat atcaacccaa gcagagcatt
     4081 acctcgtgat ccgaacgtac tacgtacttt aagtgaagat tacagcaaac tacgggggca
     4141 atgatgtcct attcccctgt tgatgcattt tctgcagcga gtggtatcga tcctttcaga
     4201 ttgaaactca tgatagctat tattttcctt tcaatgctgt tactagcagt cgcttgggcc
     4261 ttaaatagtg gctataaggg gttttcattt agagataaaa gtcctttttc acttttagtt
     4321 ttatttctca aaggagtagc actattagtt tgtgttactt atttctttat ctattagatt
     4381 cgaggtctat tatgagccgt attaaatctc ttacgaaaca tctgcatagt cgttatttag
     4441 ctgtaatgct cgtattctgg ggaactatcc aacatgcgtt tgcagccggt ccggatgcat
     4501 ccaaaatccc agacttccaa attccaggcg tggatagtaa tactaaagat ccaatggaaa
     4561 ttctttggat tgtaggtaaa agtatcatta ccttatttgc ggtattactt gccgccggtg
     4621 ccatttttgt tgtcgttaaa gccttgatca gtatctataa cgaagcaact tctgaaaacg
     4681 gcaagaaagg ctggggacac tttattgttg ctttaattat cggctttatt gtgattttct
     4741 tctgcttgtg gttagtcaaa cttgcagtcg gactcttcta attaaaatgt ctaggagacc
     4801 aatatgccag aacaagatac aaccattcct tttttgccta ctcgcctcaa tcgagaggct
     4861 accgtttacg gcggtatgac agtgagcgaa ttcggcatat ctgctggatt gggatttatg
     4921 ttaggtctga ttgttggtct cttcttgtgg atattaactg atttttggct tcttattccg
     4981 gcaatggcaa tgttgctctg tatcgcaacc gttttaattg ggaaaggtgt tgtagccgct
     5041 gttaagcgag gaaagcccga agcctactta aataggttaa ttgaaaagaa aatggatgac
     5101 ctctttaacg gtcataagtt tataagacga gaagggtttt ggtctattcg ccgatataaa
     5161 aggaaataac aaatgagcca gttaaaaagc caattacacg aaaaagataa acatatcaaa
     5221 acgactcgaa tttttgcctt cctgtttttc attattattt tggtgctcgc aggcgtgatc
     5281 tacacattcc caagtcgcct tacgatatac aacccaccag atctacgagc agggagccaa
     5341 cgagcttggt gggaaattcc taaaccaaca atttatgcat tttcttatca aattttccag
     5401 caaatgaatc gttggatgac aaatggcgaa gaggattacg agaagaatat taccgccctc
     5461 aaaccatttt taacaccaca ctgtgaagca tttttacgac aagattataa agatcgtctc
     5521 cgtaacaatg aactacgtag ccgaactcgt atggtatatg aaattaccgg tcgaggctac
     5581 agtgaagata aagtgaccgt atttagcgaa gacaattggg ccgtcaatct tgatttaagc
     5641 gtagatgaac atttccaagg acaaccggta aaacgagtct atatacgttt tcctatcagt
     5701 attgtgagaa tggatatcga ttcagtcaaa aatccttggg gattagggtt taactgctat
     5761 cactccattc caaaactctt agagggcgtt gagccaaaaa cagaggagaa aaaataatga
     5821 aacgtctacc aattttattc gcttctttac taataagctt ctctgggagt agctttgctg
     5881 atgtcttgat gaaatgggaa agaaaaccct tgcctattgc attgcaagtg gaaaaagaac
     5941 gaatcatttt tgtcgataaa aatgtcaaag ttggctatcc ggctgaatta gatggcaaat
     6001 tacgcattca atcaacgggt ggcgcagtgt acttaaaagc cctagagaca ttcccttcta
     6061 cccgtattga atttcgtgat gtttctacag gtgaaatttt cctctttgat attgtttctg
     6121 caaccaaagt agctaatccg gaagaatcaa tacgaatggt atttgatagt gcggtagaaa
     6181 aacaatcttt ttcaacgaat agagatgatg aacctgatct atctttgacc ggcacacgac
     6241 cacaattacc gataccggca gcattaaccc gttatgccgc acaatcactt tatgcgccac
     6301 tgcgtgcagt tgagccttta gaaggagttc gtcgtgtagc acatcgactt ccgaaaaaaa
     6361 ttaccacatt gctccctgcg tattcgatag aggcaacacc tttaatcagc tggcaattgg
     6421 acgaatatgt agtcacggct attcgtctac aaaacaaagg taatagtcaa attacactcg
     6481 atcctcgaga acttcaagga caattttatg ctgcaacttt ccagcataat tggcttggag
     6541 gatatggagg gaatgaagat accacaaccc tgtatttagt gacggaaggc tatgctaata
     6601 atgcgcttat tccggaaact caccctgtta aacccgtcgg taagaaagtg aaaaaaacaa
     6661 caaaagttga aactaaaccg actcaatcgg cgaagtagga ggagaagcta tgaaagcgaa
     6721 taaagccttg tttgtcatat tggggattgt gtttacccta gtcggtggga ttgtctatgc
     6781 cttattttgg cgaagtgata caccccaaga accaacagag caggaaattt tagatatttc
     6841 cattaaagat ttaacaccgg aacaagtcag atcaatgggc attcaaggtg atacgccgat
     6901 tgatactctg cgtactcttg tgacaaccgt taaatccaat gagcgtcgta tggatgaatt
     6961 aatcgatgaa aaccgacaac tcaagcagaa agaagattta gtgaatggtc gtatcagtga
     7021 tgcagtgaat aatgaaactc gcaaactaca agagcagatt gcagatttga aaatgcagtt
     7081 atttgatatg gcaacacatg ttaataacaa atcgacagca caagaatcaa ctcctcaagc
     7141 agaagctcag cctagtatga caacgaataa aggatttggt actgccggag ataaattacc
     7201 tatcggtggt gcttctatgc ctaatgtgga tattggagga atgtactggg tagagccaag
     7261 cgatcaaatt ggattggatg caggtggaaa agctgtatca ggtaatacac ttgcaactaa
     7321 gtttggattc ccgaatgatt ttaaaggtgg acaaactact ggaaataccg aaaagaaagg
     7381 tctgccgttt ttcccggaaa ctgatgtagt gacaggctcg caaggaacaa caggaaattc
     7441 tcgtggtaaa aaccagatta cgccggttta caccgtacca agtaattcca cattccttga
     7501 ttcagttgca atgacagggc ttattgggcg tatcccgatt gataataaag tctctgatgc
     7561 ttatccattt aaagtactga ttggtcgtga gaatctcatt gccaatggta ttgaattact
     7621 tgatgtagaa ggtgcgattg tttcgggtac agctactggc gactggactc tatcttgtgt
     7681 acgaggcgat gtaaaaagta tgacctttgt gttcaatgat gggcgtattg tcagcacgac
     7741 taacaaaggg aatagtgaga atatcggttg gttatctgat agttcaggca ttccttgtgt
     7801 tcccggggag agaaaaacaa atgcaccaga atatttaggc acaaatttct tcttagcggc
     7861 tgccagtgcc gcagcccaag ggttttcaca atcacaaaca acgacagtag ttgatggcaa
     7921 tagtgttgtt ggagctgtta ccggtgatca aggtaagttc atcttagggc aagccatcgg
     7981 cggtggttta aaagaatccg ccgactggtt tagaaagcgt tttgctgaaa actttgatgc
     8041 tgtctatgta ccacctggac atcgtgtcgc tattcatatc gagaaagaga ttgagatcga
     8101 ttacaactcc aaaggacgta aagtgaaata cggaaacaca tcaactaata cacaacttga
     8161 ttaaaaggaa caaacaatga aagctttata ttgcataatg atgagtgcct tattgataac
     8221 aggttgcaca acttcaactg aagaattatt acccgctaac ggcagtacga tgcgagatat
     8281 ttgggataaa ggagcaggta ataacgatct acacttatac cgttcacaac aaggacgaac
     8341 actcgatcct gtcgattata ttcctaacac tgagcaacga gcctataccc gtacggcaga
     8401 aaatgaaatt aataacttgt tccctcgttt acctaatccg gatctgatta tgtatgtgta
     8461 tccacaccaa actcgttccg gagagcctct tagtattcca ggatattcta cggtattacc
     8521 tttctacggg cgagtccaat atgctcaacc gggtgatcga acaagagggc tttgatgatg
     8581 tcttttttaa gtgaggtatt ttcctctttc aaaccgacta agacagaggt tatatcaagt
     8641 gaaccaaatg aaaattcagg tgagtctgac atcattcaaa ataaaatgag tgaggtaaat
     8701 ggcgaggttc aaccattaaa acgcagtgga aaactcaccc tgtcacagca tcaccgtcgg
     8761 ttatactcac aagccccctc ttttattgat catctacctt ggggagaata tttgaatcaa
     8821 cacggtgtga tactcctcga tgatgctcgt agtgtcgggg cggtatttga tatcaagccg
     8881 attggtacag caggacgtag tattgagttt ttaagcagga tacgaaatct tgtcaaagat
     8941 gccttacaag atagctttga tgagctggat ataaatcctt acgtcgtgca attttattgc
     9001 caagatagcg atgatttaca gccttatatt gatcagctac ggaattatat ctcgcctatt
     9061 gcccaaaata gtgcatttag cgaagaatgg ttacgagtac aagccaagca tttcaaggat
     9121 attggtaaac cagaagggct ttttattgat gacagagtga caaatacagc ttggtctgga
     9181 cgtattcgga gtaccaaaat ggtgatttat cgatatgtag ataagtccaa tgattccaat
     9241 gaaaatctta ttattgagca gcttaataat gcttgttccc gaatcaatgg ggctctaaca
     9301 acggctggat tacagttaca gcggctagat ggtgaacaaa ttcgccaatg gttattgcgt
     9361 tggtttaatc ctaatccatc tattaatttt gagggattaa gcaacaatga ttgttaccat
     9421 ctttttggca aacaaacctc aaatgatgat cttccattag cttttactga tttcagtgag
     9481 gatttatttt attcacagcc gagaaatgaa ggtagctatt ggtattttga cggacagcca
     9541 cacgaggttg ttgtcgtcaa taatttaaga aaacaaccag aagtgggctt aatgaccggc
     9601 gagattactc gtgggcgtaa tattaatgcg ttatttgacc tattgccaga atcaaccatc
     9661 atggcaatta ccatcgtcat ccatcctcag gataagttgg aagcacattt agaaaaaatc
     9721 agtagtcgag cagtgggtga gaattttgag tcaaaatacc taaaagaaga ctgtcaaact
     9781 gtacaggctt atcttaaaga gggtcataaa ctctatcaag cagctattgc attttacgtt
     9841 cgaggtaagg acgaaaagga attgcgtact cgatctcgcc aactaagaac aattctgctc
     9901 agtaacaatt tagagccggt aaaggaaaac gcagaaattg ctcctcttaa tagttaccta
     9961 cgttggctac caatgaattt taatccgcag atggatgcca aatcccatta ctacacgaag
    10021 tactattttg tacaacattt ggcaaactta ttgcctatct ttggtcgaga tacaggcact
    10081 ggtaatccgg ggattactta ctttaatcga ggaggagctc cgtttaattt cgatccgttt
    10141 aaccgtaaag acagaaccaa aaacgctcac aaactcttat taggaccaac aggctcagga
    10201 aaatcagcaa cactatgtgg gcaattatgt caggctatgg ccgtttatcg tcctcggacg
    10261 tttgttgtgg aggcaggaaa ttcatttggt ttattagcgg actatgcaga acgttttgga
    10321 ctgaccgtaa atcgcattac gttagcccca aacagtggca ttgtcttacc gctcttttca
    10381 gaagcccatc ggttattgga tatggaatta aatccggaag atgtgccgga agacattgag
    10441 gataacgatg acactgatga tgaaggcgat atgcaacgag atttgttggc cgaaatggag
    10501 ctgacagcac gtttaatgat cactggtggc gaaaagaaag aagatgaaaa aatgagccgt
    10561 gctgacagag cagccattcg acgtgccatt ttaaatgcgg ctcgccaaac taaatccgaa
    10621 ggcagacaaa cgctaactga agatgtgaaa aaggcattgg aggctataag ccgtgatgaa
    10681 aatactcgtg aagaacggcg tactcgtttg gccgaaatgg cagaatcatt ggaaatgttc
    10741 tgtaccggag tcaatggaca atttttcaac cgggaaggcg aaatttggcc ggaagcagat
    10801 ctgacattgg ttgatctagc catgtttgcc cgggaaggtt atgaagcaga attggccatt
    10861 tcctatattt cactcattaa ccatatcaac agtctcggtg aaaaatacca gcatagcgac
    10921 aggatgattc tgaatgtaac cgatgaatca cacattatca ctgccaaccc attattagcg
    10981 aaattcttgg tgaaaggctc aaaaatgtgg cgtaaattag ggatttggct gtggcttgcg
    11041 acacagaata tggatgactt ccccaatgaa gcagcgaaat tgctctcaat ggtcgaatgg
    11101 tgggaactgt tgaatgtgtc tgctagtgaa attgatgaaa tcaatcgctt ccggaaactg
    11161 tccgaagctc agaaaacgat gttgttatct gccaaaaaat cagatcgtaa atataccgaa
    11221 ggtgtggtat tggcaacaaa tatggaagcg ctattccgtg ttgtaccacc gagtttattt
    11281 ttggctttag gaatgaccga gaaacacgaa aaagcagaac gtcggcaatt aatgatgcaa
    11341 catggttatt ctgaattaga tgctgcttta gaagtcgctg ctcgtattga taaagcgagg
    11401 ggaattgcat aatgatgaaa caacactatc gactatttaa tgtcggacca caaaaatcct
    11461 cattagggat tgtatcttcg gcggtagaaa ctcaactgct tggattttgt gaacaaattc
    11521 gccaactcat cggtatcggg aagcagatta ccttattccc tcatcgggta gtgcaaaacc
    11581 actgggctat cgaacttagc ggcgagaaag gctctgttca cttattacta aatgtgacgg
    11641 ggcgttcttt actgcctact tccaattacc gtgaatggcc tgctcgttta gtcatcgaaa
    11701 tagccgatca tgtggatgct tactggtttg caacattcct tgctcagcaa ttggatttga
    11761 aaagtaatct tttcttctta tccacatcaa ttgtggataa ctctatgcat aacgaggtaa
    11821 cggatactaa tgaagcctga tacgacaatg cctaaaatga aaaaggtgag agctgtatta
    11881 aaaacaacct tgattatcat cggtattatc ttactactac ttattgttgg ttggctggca
    11941 atgacctatt tccctaccac aaattttcaa gcgtggttct atgaaacccg ttatgcatgg
    12001 ttgggatggc gatttgtatt atatggggtt atttggttct taatggtaaa actcaatcga
    12061 agaggacaaa tgccattgaa agccagactg atgattttat ctgctatccc ccttatagag
    12121 ggaattaatt tactgtatct actttaagga ggatgagatg acctttaccg tagatagcta
    12181 cttagaatac tttttgacct tactagcttg gatcattaac aataatattt ttgctgtact
    12241 ggttcaaacc ggtctattca tactaccgct gatttttgtt ttaatcggca catggatgga
    12301 agttaaaaag caaggggagg acgaaggtaa taaaggagat ttacttatta cttggctcag
    12361 ccttaaattt tatccggcga tgtttgttgt agtgttagtt ttggccccga tgatacctat
    12421 caaccttaac aatattgagc tgaatgttga acgttccaaa gcctgtggct accgtattcc
    12481 aacctcacca gaggatagtg gttataatca aatgaactct gaattgggca accagcaagc
    12541 gagagtaccg ctttggtggg gattattcca tcaaatcaac aaaggagtta cacacgcatt
    12601 aacagcttca attccgtgta aaccggattt acgtcaagtt cgctttgagg tccaacatga
    12661 aaaaatcaat aatcctgcat tattgactga ggttcgctta tttgtacaac aatgttatgt
    12721 acctgcacgt cagaaaattc aaagcagtca gctctcattg actccggcac aggtgcgtga
    12781 aaccagctgg ttaggtggtc gtattctttt gacaaacagt gagctttatc cccgttatcg
    12841 agctcaacaa cctcatagtc tatttcccta caatgcttcc cgggataatg gattgccgaa
    12901 taatggggaa ggtggttttc caaattgtgc agagtggtgg gatacggcga atactggttt
    12961 aaaagatcgt ttattagctg atgtcaaaca gaacttctct gcaaaagtga ttgatttttt
    13021 tagcagcaaa gaaaatgcgg atgaatccat actccgtact gtattacgag cagagaatat
    13081 ggatatttcg agagggaaag tttatcccgg gtatgggggg aatttagatc ctacatttgc
    13141 aaatgcaacg actcgattaa catcttcagt gggaagctta gctgccagct ttgcagcctt
    13201 tccagcattc gatgcaatgc gacaagcact gccgatggta catgctttta tgttgatggc
    13261 tgtgatcatc attactccgc tggttattgt gatgagtggt tattctctca aaacattagt
    13321 tacattaact tttgtgagtt ttgctttggt atcactgaca ttctggtggg aattagcaag
    13381 atggttagat tcttggctac tatccgctct atatgattcc agtgcacata gtcgaataaa
    13441 tccgtatttc cttgaaaata cgcaagatga ttttatcgtc aattttgtga tgggttcgat
    13501 gttcttggtt ttaccggcga tttggctagg cagtatgtcg tgggctggag taaaaatagg
    13561 agatatagct gccggtttta ctactgattc attagatagt aaatccgcag gctctcaagg
    13621 tggtaattta gttaagaaag gcggtaagga aatatctgca gggtttaaca aataaaacaa
    13681 taaggtagag gttaatctct accttattaa tttctaatca tattttctca tctttttaca
    13741 aaatagccac cctgatagag tccatatcca aaatatccat cacgatatcc atcaagctga
    13801 ttttcgtctt gatcagcaat tacatcccat aaatcttccg gcttactatc ctcactatca
    13861 tcttcaattt cattaaccgt gttttcgacg tggtttggat tatatgctac gtcatttgtt
    13921 gaaatatctt ctactggtgt tccattgaga attcgcagaa ccgtgaatcc aactaaaaac
    13981 aagggaagtc ctgcaaaaat caccgcaaaa acgacagctt gttgtagcgg ttttggtaaa
    14041 aatgcggtac gttgtttgat aaatttaacc ggcttagcga gctgctttcc gaaatcgata
    14101 aatttcatag tatctttcct aagataaaag gttattg
//
LOCUS       DANLRK010000054        14124 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.054, whole genome shotgun sequence.
ACCESSION   DANLRK010000054 DANLRK010000000
VERSION     DANLRK010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 14124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14124
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.054"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(<1..633)
                     /locus_tag="PXA33_001142"
     CDS             complement(<1..633)
                     /locus_tag="PXA33_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HDL5120480.1"
                     /translation="MAEGLLLTPYFQTEVGIVFYKVPKDVSPAAFGERTLLQPAPTDL
                     ATVKSGKVANFSANSTACKELAVFAKSAKVRSTLNQRDKYSNDLPYIRFVKSIQTCQC
                     RDGKYCHTEKVLVENKNGAVQLCWHHDKMRMDGEIPNEKLEALADENWEKFIAQQIRL
                     TLRKTQSTAIEFADLVLFASIKGLLDELDSKALQQFLGYPPLNNSGVSKES"
     gene            complement(760..1353)
                     /locus_tag="PXA33_001143"
     CDS             complement(760..1353)
                     /locus_tag="PXA33_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine methyltransferase"
                     /protein_id="HDL5120481.1"
                     /translation="MTDFDKNTYQTPNYVRNWLNHRYAWFHIDGCSNGQNTLYTYWIG
                     RAADGLDDDKLSCQIADDFLADDLFDVLLDKVSDWGELLRIFVNPPYSDPLPFVQRAA
                     ELKKAGHLVVMLLPADKTTEWYTIIQQHANEVIDIIGYHDEKGTWRTGRIQFINPVTG
                     KPAQGNNKGSMIVVFDPFIEGIVTRQMPLDKIKEWGK"
     gene            complement(1364..2410)
                     /locus_tag="PXA33_001144"
     CDS             complement(1364..2410)
                     /locus_tag="PXA33_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HDL5120482.1"
                     /translation="MRFTSTINNVRLVEWEINITQGALVDLINQASSWAKAVVIDGIT
                     YYWMSFSKVCEELPAVFSKEDTVYRQYKVLKEKGIIDHFKMDGKDYVRLTEKGCEWNK
                     FEPIRESEKNPSIGNNSEQTRKNFRESSEKNPTDNNTNYKNNNDHTTPLNPPAGEPAP
                     AEVVLNYLNSALVTLAVQLGERKPVGYSLKPWAKNITARIRESSVADCCQVVDYLVAK
                     WGRDEKMREYLCPKTIFRQSNFADYFPKSTAWANNGKPVCVNGKWVTPAELEKRLIMP
                     TVDEVRALFQKSLSGNPFKALDFTNKRNLVMYHATINTRNKRPLERDLPMIISQEIKN
                     AVERIDRLRVPTFS"
     gene            complement(2407..3090)
                     /locus_tag="PXA33_001145"
     CDS             complement(2407..3090)
                     /locus_tag="PXA33_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein/antirepressor Ant"
                     /protein_id="HDL5120483.1"
                     /translation="MNQLLNISAPKASITMSSREIAELCEKEHRHVTRDIEAMFSQLD
                     IPAEGYAHFWTHPQNGQTYREFRLPYDLTLTLISGYSVILRKKIIDRWQELESRQGIP
                     QSFSQALRLAAEQQEVIEQQNHQLAIQAPKVAFVEHYVEVGTTKSLRETAKILNFPEK
                     RLIECLERYRVLYRQSGNLLPYQDKQAKQLFAVKTGTAEHGHNFTQTRVTAKGIEWIA
                     QRYASELGQ"
     gene            complement(3148..3336)
                     /locus_tag="PXA33_001146"
     CDS             complement(3148..3336)
                     /locus_tag="PXA33_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HDL5120484.1"
                     /translation="MKKDDVLEHFGTLEKVAATLGISVSAVSQWGEIIPEKNAYRLQE
                     ITAGKLKVKHSLYRNRSK"
     gene            3461..4117
                     /locus_tag="PXA33_001147"
     CDS             3461..4117
                     /locus_tag="PXA33_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL5120485.1"
                     /translation="MNTQTLGDRIKSRRKELKLTQKDLATALKGVSHVAVSQWESSTT
                     KPNSENILDLSTVLQCDISWLLRGNGESNVLPASLGMTKVPLISYIQAGQWTGIDDFR
                     ETCGDYEYILTDLEVSEDAFALEIKGDSMEPDFIEGDRVIIDPDVQPHAGEFVAAING
                     DYEATFKKYRPLEDLDEYGRQHFELIALNPDYHKLSTLKQEIRIIGTMVEHRIYRRKR
                     "
     gene            4129..4503
                     /locus_tag="PXA33_001148"
     CDS             4129..4503
                     /locus_tag="PXA33_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5120486.1"
                     /translation="MTEFKDVKQAIVEAQLASTPKDKVVNDIMKGSAELLSANDIINR
                     LGIGADEFQELVNLPNHYLRINGGLGAAVYNTRRMFDTHVELVNQELEKNISFPKPDL
                     YILGKARWKKETFKKWLEEQCR"
     gene            4655..5392
                     /locus_tag="PXA33_001149"
     CDS             4655..5392
                     /locus_tag="PXA33_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HDL5120487.1"
                     /translation="MLSKQVLSISESRLSTYLFCFFRNDPTKQKEAIAIYTALQHRTG
                     IYFSLIQEIEVALRNEVSQLLRFAAPNHNLYEFFHFLANDSNAPLNAESKRQLQKAIS
                     ECGRNYNENDIISHISFGFWVNLFNYDIKRNQYVAYWQSVLKPIFGHRFSNFKDLYNT
                     LKQIMRFRNRLYHQEVVWNKRIAKKPTHALENLEKTYKQFELALQKIAPERFAFRQLS
                     QALIWQRQIFFDQQIFEAEIVILPQHI"
     gene            complement(5428..6024)
                     /locus_tag="PXA33_001150"
     CDS             complement(5428..6024)
                     /locus_tag="PXA33_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5120488.1"
                     /translation="MKKLLMTLCCLPAFALANETGESCSKIEDSAKRLECYDSVFVKK
                     DTGKDEATDEKSNWEYEQKKDELRNATTYFAKIRSTNTIDFGFPYNSSSMNLMLRKDP
                     KYGNDVIFSVHGQFNGCMIESCKITVKFDDGKLESYRMIGADGGSNDTLFIENAKAMK
                     TFVDKLKKSKKLIVEASFYNYGKGQFTFDTQGLEWKHF"
     gene            6670..6855
                     /locus_tag="PXA33_001151"
     CDS             6670..6855
                     /locus_tag="PXA33_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5120489.1"
                     /translation="MNKMKQLAPFALLILLLGIVGRMDYDDHIQMQRYKCERNQGFWQ
                     MESNGNQYCGGKYGTKN"
     gene            6839..7036
                     /locus_tag="PXA33_001152"
     CDS             6839..7036
                     /locus_tag="PXA33_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5120490.1"
                     /translation="MARKIDAGVEIVEVDKRFIAKFFYDGKLQHTTYPQYNRQNAIML
                     INRKIERFNIGRDKPIKLYKE"
     gene            7039..8013
                     /gene="ssb"
                     /locus_tag="PXA33_001153"
     CDS             7039..8013
                     /gene="ssb"
                     /locus_tag="PXA33_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDL5120491.1"
                     /translation="MAGINKVIIVGRLGNDPELRTMPNGDSVAKISVATSTEWTDKTS
                     GEKKQATEWHSIIAFRNLADIIGKYLKKGSQVYVEGKLRTRKWQAQDGTDRWTTEIIA
                     DQLQMLGSSNSGNSNNNWATEPAGNPPPTNPYNHVMTDSESRDFDDDIRVRWITKEQA
                     KQFINNISTDWMKTICSFALLTGARRTEILSMTWDKIDFIRKVAIVSNDIAKSEKARS
                     LLLNDDAIELLQKQRGKNPKYVFVGSKGQALQDINRKIFNKATEKCLLVDFHFHDLRH
                     TWASWHVQAGTPLFTLKELGGWETLEMVKKYAHLNADHLLNHANSVKF"
     gene            complement(8100..8176)
                     /locus_tag="PXA33_001154"
     tRNA            complement(8100..8176)
                     /locus_tag="PXA33_001154"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8140..8142),aa:Arg,seq:tct)
     gene            complement(8201..8277)
                     /locus_tag="PXA33_001155"
     tRNA            complement(8201..8277)
                     /locus_tag="PXA33_001155"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8241..8243),aa:Pro,seq:tgg)
     gene            8447..9307
                     /gene="folD"
                     /locus_tag="PXA33_001156"
     CDS             8447..9307
                     /gene="folD"
                     /locus_tag="PXA33_001156"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HDL5120492.1"
                     /translation="MTAQIISGSEVSSQIKKKVSEKIAHYLEQGKRNPGLAVILVGND
                     PASQVYVGSKRKSCAEIGIASKSYDLPEETTEAELLALIEKLNQDDSVDGILVQLPLP
                     KQIDSTKVIEAILPHKDVDGFHPYNVGRLSQRIPTLRPCTPYGVMKLLETTGVDLYGQ
                     HAVIVGASNIVGRPMALELLLGGCTVTVTHRFTKDLEHHVRQADILVVAVGKPEFIPG
                     EWVKDGAIVIDVGINRGADGKLKGDVEYSVASQKAAFITPVPGGVGPMTVAMLMQNTL
                     QTYENHLQAV"
     gene            9421..10872
                     /gene="thiI"
                     /locus_tag="PXA33_001157"
     CDS             9421..10872
                     /gene="thiI"
                     /locus_tag="PXA33_001157"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HDL5120493.1"
                     /translation="MKFIIKLFPEIMIKSDSVRKRFIKILTSNIRNVLLKETENVSVV
                     RHWDFIEVRAKVDEETALVLDLLKRTPGIHHILEVEETPFTDMHDIFEQTYAAVKAEL
                     EGKTFSVRVRRKGKHDFRSLDVERYVGGGLNQRIETAKVKLTNPDVTVRIDIEHDKML
                     LIKARHEGLGGYPIGTQEDVLSLISGGFDSGVSSYMLIRRGSRVHYCFFNLGGAAHEI
                     GVKQMAYHIWSRYSTSHKVRFIAINFENVVGEILEKVDNGQMGVVLKRMMVRAASRIA
                     ERFDIQAIVTGEALGQVSSQTLTNLRLIDKASDTLVLRPLITHDKEQIIAMAKAIGTD
                     DIAKSMPEFCGVISKNPTVKAIETKILEEEGNFNFEVLEQAVQTASYLDIREIAQQTE
                     KEVVSVETTTELSKNDVVLDIRSPEEVDEKPFRLDGVEVKELPFYKLSSQFSTLDQSK
                     TYLLYCQRGVMSKLQALYLKENGYSNVKVFRVK"
     gene            complement(10917..11624)
                     /locus_tag="PXA33_001158"
     CDS             complement(10917..11624)
                     /locus_tag="PXA33_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwiC-like family protein"
                     /protein_id="HDL5120494.1"
                     /translation="MFKEKPVISNQHGALAMAMLPFLYAVIESGFSTLHIFFGLSWLF
                     LYFFSYPFLSLFSKKPTERNKKWAKIYFGLSILFALPLIYAQPSILQFLPIILPLGLV
                     QVHYAKQKDERNLWNDIAGILTFGIMGMAAYYLVTKQYQFEILLHPTLFFIATTFYIK
                     SMVRERRNPIYMEISIAIHLVLAMLYILCSLQAVFFAYLIATARAIIVPSFGWNVKQV
                     GMLEFPVILLFLASLIW"
     gene            complement(11692..12666)
                     /gene="pfkA"
                     /locus_tag="PXA33_001159"
     CDS             complement(11692..12666)
                     /gene="pfkA"
                     /locus_tag="PXA33_001159"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007523004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HDL5120495.1"
                     /translation="MSKKIKKIAVLTSGGDAPGMNAAIRGVVRTALNEGLEVCGIQDG
                     YYGLYHDKVIPLDRRSVSETINRGGTFLGSARFPDFKRPEVRKHCVETLKKYSIDALV
                     VIGGDGSYMGAKLLTEEFGYPCIGIPGTIDNDIVGTDYTIGYQTALETALDAIDRLRD
                     TSSSHQRISIVEIMGRHCGDLTLSAALAGGCEYVIVPEKGLDKDALMRSIEEGFHKGK
                     RHSIIAITELMTDVHALAKEIEARFGNETRATVLGHIQRGGSPCAFDRILASRMGVYA
                     VELLLQGFGGHCVGIQNEQLVHHDIIDAINNMRRPFKEELFEASRKLF"
     gene            complement(12773..13969)
                     /locus_tag="PXA33_001160"
     CDS             complement(12773..13969)
                     /locus_tag="PXA33_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDL5120496.1"
                     /translation="MLNTQFRTHFPFFEKSDGWTYLDSAATTLKPDVLIEATSEFYAS
                     AGSVHRSQYDLPQTEQYELARDLVAKRFNVETREAVIWTSGTTQSINTVAYGLEHQIA
                     AGDEIVISVAEHHANFIPWQQLAQRTRAKLVVLPLNDKYEIELETLKNALSEKTKIVA
                     FNLVSNVTGVRQPAEQIIPLIRQSCSAKIVLDIAQAVCSEQVDVQKLDADFYAFSAHK
                     MYGPNGVGVLTGKLQSLEQIQPLVFGGKMLKNITESELTVAELPYRLEAGTPNIAGII
                     GFGQVLAWLEKWDFVALNHQLYDLAEYTCKRLNSYQNLQIIGQQNTPTISFVVKNQHP
                     ADIAAFLTQSKIAIRQGEHCAKPYLRHLEEVGTVRLSLAHYNNQQDVEHFFNALDFTL
                     EILQES"
BASE COUNT         4300 a         2795 c         2854 g         4175 t
ORIGIN      
        1 actttctttg ctcacaccgc tattattcag tggtggatag ccgagaaact gctgtaatgc
       61 cttgctgtct aattcatcaa gcaaaccttt gatactcgca aacaacacta agtccgcaaa
      121 ttcaatagcg gtagattgcg ttttgcgtaa ggttaagcgg atttgttggg cgataaattt
      181 ttcccagttt tcatccgcta gtgcttctaa tttttcattc gggatttcac cgtccattcg
      241 cattttgtcg tgatgccaac aaagctgtac cgcaccgttt ttattctcta ccagcacttt
      301 ttccgtgtgg caatatttgc catctcggca ttggcacgtc tgaattgact ttacaaaccg
      361 gatataaggc aaatcattac tatatttgtc acgctgattt agcgttgaac gaacttttgc
      421 cgatttagca aacaccgcca gctctttaca agcggtcgaa tttgccgaaa aatttgcaac
      481 cttgcctgat ttaaccgtcg ctaaatcggt cggtgcaggt tgcaacaaag ttcgctcacc
      541 gaaagcagca ggagaaacat ctttaggcac tttgtaaaac acaataccca cttcggtttg
      601 aaagtaaggg gtaagtagca aaccttccgc cattattgcc cccatttctc ggtttcatca
      661 taggctttca cgaatagcat aaatacgcca atacagatta aaaaaatcag tacgccaatt
      721 actgcaccaa taccaataaa tagttcccaa taatccattt tatttacccc attccttgat
      781 tttatctagt ggcatctgac gagttacaat gccctcaata aatgggtcaa acacaacaat
      841 catcgagcct ttattattac cttgtgccgg tttacctgtt accgggttga taaattgaat
      901 ccgccctgtc cgccaagtgc ctttttcatc gtgatagcca ataatatcaa ttacctcatt
      961 agcgtgttgt tgaatgatgg tgtaccattc tgttgtttta tccgctggta ataacatcac
     1021 aaccaaatga cctgcttttt tcaattctgc cgctcgttga acaaaaggca gtggatcaga
     1081 atatggcgga ttaacgaaaa tacgcagtaa ttctccccag tctgaaactt tatctagtag
     1141 cacatcaaat aaatcatcgg ctaaaaaatc atcggcaatt tggcaactta atttatcatc
     1201 atctaaaccg tctgccgctc taccaatcca ataggtgtac aacgtatttt gtccgttaga
     1261 gcaaccgtca atatgaaacc aagcataacg atgatttagc caattacgca cataattagg
     1321 tgtttggtaa gtgtttttat caaaatctgt cattgtctaa tccttacgaa aatgttggta
     1381 ctctcaaacg gtcgatacgc tcaaccgcat ttttaatttc ttggctgata atcatcggca
     1441 aatcacgttc aagtggtcgc ttgtttctgg tgttgattgt ggcgtggtac atcactaaat
     1501 ttcgtttgtt ggtaaagtcc aatgccttaa acggattgcc actgagtgat ttctggaaca
     1561 aggctcgtac ttcgtccacc gttggcataa tcaagcgttt ttccagctcc gccggtgtaa
     1621 cccacttacc atttacgcaa acaggctttc cgttattagc ccaagcggtc gattttggaa
     1681 aataatctgc aaaattcgat tggcggaaaa tcgttttcgg gcagaggtat tcacgcattt
     1741 tttcatctcg tccccattta gcgacaaggt aatccaccac ttggcaacag tccgccaccg
     1801 aactttcacg aatgcgagca gtaatatttt ttgcccacgg cttgagcgaa taccccacag
     1861 gcttacgctc accaagctgt accgccaacg ttaccaaagc cgaattgaga taattcaaca
     1921 caacttcagc cggggcaggt tcccccgctg ggggattaag gggggtagta tgatcattat
     1981 tatttttata attagtatta ttatctgtcg gatttttttc cgagctttct cggaaatttt
     2041 tccgagtttg ttcggagtta tttccgatac tcggattttt ttccgactca cggatcggct
     2101 cgaatttatt ccattcacaa cctttttcgg ttaaacggac ataatcttta ccgtccattt
     2161 tgaagtggtc gataatgcct ttttctttca acaccttata ttggcggtaa actgtatctt
     2221 ctttgctaaa aaccgcaggt aactcttcgc aaaccttgct aaatgacatc cagtaatagg
     2281 ttatgccgtc aataaccacc gctttcgccc aactggaggc ttgattgatt aaatcgacca
     2341 atgccccttg tgtaatattg atttcccatt ccactaagcg gacattgttt atggttgatg
     2401 taaatctcat tgtcctaact ccgatgcgta acgttgagca atccattcaa tccctttggc
     2461 ggttactctc gtttgggtaa aattatgacc gtgttctgcc gtgccggttt ttactgcaaa
     2521 aagctgttta gcttgtttat cttgataagg gagtaggttt cctgattggc gataaagaac
     2581 tcgatatcgt tctaaacatt caatcaaccg cttttctgga aaattgagta tcttggcggt
     2641 ttctcgcaat gatttagtcg ttcccacctc aacataatgc tccacaaatg cgactttcgg
     2701 ggcttgaatg gctaattgat gattctgctg ctcaatcact tcttgctgtt ctgccgccaa
     2761 ccgtagagct tgagagaaag attgtggaat accttgtctg ctttctagct cttgccaacg
     2821 gtcgataatt ttcttgcgaa gtatcacgct atagccggaa attagggtaa gggttaaatc
     2881 gtaaggcaat cgaaattccc ggtatgtttg cccattttga gggtgtgtcc aaaaatgggc
     2941 atacccctcg gcaggaatat ctaattgact aaacatcgct tctatatcac gagtaacgtg
     3001 tcgatgttct ttttcgcaca actcagcaat ctcacgacta ctcatcgtaa tgcttgcttt
     3061 tggtgccgaa atgtttaata attgattcat cgaaaatacc tttcgtgttt aattatgtat
     3121 gaaagccacc gttacagcgg tggtttttta tttgcttcta tttctataaa gtgagtgttt
     3181 gacctttaac ttgccggcag tgatctcttg taatctgtac gcatttttct caggaataat
     3241 ctcgccccat tgagaaacag cagatacact aatccctaaa gttgccgcca ctttttctag
     3301 agttccgaaa tgttcgagca catcatcttt tttcatattt tcttaattcc tttcttgaat
     3361 tttatttaag acctcttaaa tatacaatta aaggaaactt aagtcaataa atgttaagat
     3421 aacttaacag caactttatt tttaccttta aggggatact atgaatactc agaccttggg
     3481 cgatagaatt aaaagccgta gaaaagaatt aaaattgaca cagaaagatc ttgctactgc
     3541 gttaaaaggg gtctcacacg ttgcagtatc tcaatgggaa agtagtacga ctaagccaaa
     3601 ttcagagaat attttggatt tatctacagt attgcaatgc gatattagtt ggctcttgcg
     3661 tggtaatgga gagtctaatg ttctacctgc tagcctcggt atgactaaag tcccattaat
     3721 tagctatatt caagccggac aatggacggg aattgacgat tttcgtgaaa cctgtgggga
     3781 ttatgaatat atcctaactg atcttgaggt gtctgaagat gcttttgccc tagaaatcaa
     3841 aggggactcg atggagcctg attttattga aggagaccga gtaattattg atcctgatgt
     3901 acaacctcac gccggtgagt ttgtggcagc aattaatggc gattatgaag ccactttcaa
     3961 aaaataccgt ccgttagaag acttagacga atacggaaga caacattttg agcttatcgc
     4021 tcttaatccg gattaccata aattatcaac gctaaagcaa gaaatcagaa ttatcggcac
     4081 aatggtagag catcgtattt atcgtagaaa gagataggaa ttgataatat gacagaattt
     4141 aaagatgtaa aacaggcgat tgtagaagct caattagctt cgacacctaa agataaagtc
     4201 gttaatgaca taatgaaagg aagtgcggaa ctgctttcag caaatgatat tatcaatagg
     4261 ttaggtattg gggcagatga gtttcaagag ttggttaatt tacctaacca ttatttgcgt
     4321 ataaatggag ggttaggtgc tgctgtgtat aatacaagaa gaatgtttga tacccatgtt
     4381 gagttagtca atcaagagtt agaaaagaat atttcatttc ctaaacctga tttatatatt
     4441 ttggggaaag ctcgttggaa aaaagaaaca tttaagaaat ggttggaaga acagtgtaga
     4501 taaagaaaag gtaggttaaa aaacctacct tttctgaacc gcaaataagc gattctcaca
     4561 ctttacaaag caatccagct cctacgtctg gggcttcggg tccgttgcca gtgcaagtat
     4621 tattacatta aactaaactt gagtaaagga aaatatgttg tccaaacaag tgctaagtat
     4681 ttcagaaagt cggctgagta catatttatt ttgctttttc cgaaatgatc ccacaaaaca
     4741 gaaagaagct attgcaattt ataccgcctt gcaacatcga acaggaattt atttttcatt
     4801 gattcaagaa atagaggtgg cactaagaaa tgaagtcagc cagttgttac gctttgctgc
     4861 acctaatcat aatttatacg aattctttca ttttctagcg aacgatagta atgctcctct
     4921 taatgcagaa tctaaacgcc agctacaaaa agccattagt gaatgcggac gtaactataa
     4981 tgaaaatgac ataatttctc atatttcttt tggattttgg gtaaatctgt ttaattatga
     5041 tataaagcgt aatcaatatg ttgcttactg gcaaagcgta ttaaagccta tttttggaca
     5101 tagatttagt aattttaagg atttatacaa tacattaaag caaattatgc gttttagaaa
     5161 tcggctgtat catcaagaag tggtttggaa taagcgaatt gccaagaaac caacgcacgc
     5221 actagagaat ttagaaaaaa cttacaagca atttgagtta gcactacaaa agatagcacc
     5281 tgaacgattt gcgtttcgtc agctttcaca agcacttatt tggcaacgcc aaatcttttt
     5341 cgaccaacaa atttttgaag ctgaaatcgt tattttgccc caacatatct aagcaaaaag
     5401 ctacgcatct atgtagcttt ttcttaatta aaaatgtttc cactctaacc cttgtgtatc
     5461 aaaggtaaat tgccctttgc cataattgta aaaactcgcc tctacaatta gttttttcga
     5521 ctttttcaac ttatctacaa aggttttcat tgcttttgca ttttcaatga aaagcgtatc
     5581 attgctgcca ccatccgccc caatcattcg ataactttcc aatttaccat catcaaactt
     5641 cacagtaatt ttacagctct caatcataca gccattaaat tgaccgtgta cgctaaaaat
     5701 tacatcattg ccatatttag ggtctttgcg taacattaaa ttcattgatg atgaattata
     5761 cgggaaacca aaatctattg tgttagttga tctaatttta gcaaagtaag tcgttgcatt
     5821 acgcaattca tctttctttt gttcatattc ccaattagac ttttcatcgg tagcttcatc
     5881 tttaccagta tcttttttca caaagacact atcataacat tctaagcgtt ttgcactatc
     5941 ttcaatctta gagcaacttt ctccagtttc gttagctaaa gcaaaagcgg gcaaacaaca
     6001 taaagtcatc agtagttttt tcatataacc tccatagaaa tcccttctta ttttctcaaa
     6061 agcgtggttt tcggtctgtg atcaagctcg caatctcaat aattatattt taatggttta
     6121 aaaaacaatc aattaagaaa tcataactaa ttgaaattta agaaatctta aaaatactta
     6181 ttaaataagc ttaaattttg attgacttgt aatttaagtt ttcttaaact taattcatca
     6241 aaacgagcta cggctcaatg ctctttaaca attcggtgct tgtgcggacg atattaacaa
     6301 cctcgggcag ttgtaaagta atgctttata actcagtaag gttggttaga ccctgacata
     6361 caaatttgaa cgacttgcca aaaagaggtg gcaaggttta ggcaacacac tcgcaaggtg
     6421 tggagagctg aaaaggcaac actggtatca gtcctaagca atcacgctta tctcaagttt
     6481 gagtaagtac ggatacaagc agcacccgac ggaatggcga aagccgggcg ggcgacggcg
     6541 tgggcagaca cgcatttatt ccaaagctca tttcaccaag tgtgtttcgg aatacccaca
     6601 actagacgca agtcgctcca tttgcccacc gtaaaacgtg ggcttttttt aaccctaaag
     6661 gacaatccta tgaacaaaat gaaacaactt gccccatttg ccttgctaat tttgctatta
     6721 ggcattgtcg ggcgaatgga ttatgacgac cacatacaaa tgcaacgtta caaatgcgaa
     6781 cgaaatcaag gcttttggca aatggaaagc aacggcaacc aatactgcgg agggaaatat
     6841 ggcacgaaaa attgatgccg gtgttgagat tgttgaggta gataaacgat ttatcgccaa
     6901 gtttttttat gacggcaaat tacagcacac aacctatcca caatacaacc gccaaaacgc
     6961 aattatgttg attaaccgca aaattgagcg gttcaacatt ggcagagaca aaccaatcaa
     7021 actttataag gaataacaat ggcaggaatt aacaaagtga ttattgtcgg tcgcctaggt
     7081 aacgacccgg aactacgaac aatgccaaat ggcgattcag tcgccaaaat cagtgtagca
     7141 acttcaaccg aatggacgga taaaacctca ggcgagaaaa aacaagccac agagtggcat
     7201 agcattatcg cctttcggaa tttagctgac attatcggca aatacctgaa aaaaggctcg
     7261 caagtgtatg tggaaggcaa gctacgcact cgcaaatggc aagcccaaga cggcacagac
     7321 cgctggacga ccgaaatcat cgccgatcag ctacaaatgc tcggtagctc aaacagcgga
     7381 aacagcaata ataattgggc aaccgaaccg gcaggaaacc caccaccaac caatccatat
     7441 aaccacgtaa tgacggattc agaatcacga gattttgatg atgatatccg ggttcgttgg
     7501 attaccaaag aacaagccaa gcagtttatt aataatatta gtactgattg gatgaaaact
     7561 atctgctctt ttgctttatt aacaggagca aggcgaacag aaatactttc tatgacttgg
     7621 gataaaatag attttatccg taaagtagcg attgtttcga atgatattgc caaatctgaa
     7681 aaagcgagat cgcttttact taatgatgat gcaattgaat tattacaaaa gcagagaggg
     7741 aaaaatccta aatatgtttt tgtaggaagc aaagggcagg cattacaaga tattaaccgc
     7801 aaaatattta acaaagcgac tgaaaaatgt ttattggttg atttccattt ccatgattta
     7861 aggcatactt gggccagttg gcacgttcag gccggcacac cattatttac cttaaaagaa
     7921 ttgggtggct gggaaacatt agagatggta aagaaatatg cccatcttaa tgcagaccat
     7981 ttactcaacc acgctaactc agttaaattc tagtcacaaa ttagtcacaa agtatttttg
     8041 acataggaaa ttaataactg atatttaaat gtaactattt gaaatataag ggtaattaat
     8101 ggcacgccct aaaggattcg aacctttgac ccacgcctta gaagggcgtt gctctatcca
     8161 gctgagctaa gggcgcagat gaagataata taaaaagaag tggtcggcga gataggattt
     8221 gaacctacga cccactggtc ccaaaccagt tgcgctacca agctgcgcta ctcgccgaca
     8281 taagatgttg ttgattatag atttctttgc acaacagtca atcttttttt gtgaatgtcg
     8341 gcttaaatga ttgaagttta tccgttattc aaaattagct tatcaaaaag gctatttttt
     8401 gagagaatga cgaatgtttt gccaagtgat atttcgggag gtagtaatga ctgcacaaat
     8461 tatttcgggg agtgaggttt cttcacaaat taaaaaaaag gtgagtgaga aaatcgctca
     8521 ttatcttgaa cagggtaaac gtaaccctgg tcttgcggtc attttagtgg ggaatgaccc
     8581 agcctctcaa gtgtatgtgg ggagtaaacg caaaagctgt gccgaaattg ggattgcgtc
     8641 gaaatcttat gatttacccg aagaaaccac agaggctgaa ttattagcac tgattgaaaa
     8701 gttgaatcaa gatgattcgg tcgatgggat tttagtccaa ttaccgttac ctaaacagat
     8761 cgattcgact aaagtgattg aagccatctt accacataaa gatgttgatg gattccaccc
     8821 ttataatgtt gggcgtttat ctcagcgtat tcccacttta cgcccttgca cgccatacgg
     8881 tgtgatgaag ttgcttgaaa caactggggt agatttatat ggtcaacacg cagtgattgt
     8941 aggtgcgtct aatattgttg gtcgcccgat ggcgttagaa ttgttgcttg gtggttgtac
     9001 agtcacagtt actcatcgct ttaccaaaga tttagagcat cacgttcgcc aagcagatat
     9061 tttagttgtg gcagtcggca agcctgaatt tattcccggg gagtgggtta aagacggtgc
     9121 tatcgtgatt gatgtaggga ttaaccgtgg agcagacggc aaactaaaag gtgatgtaga
     9181 gtattcagtt gcctcacaaa aagcggcatt tatcacccct gtccccggtg gggtaggccc
     9241 gatgacggtg gcaatgttaa tgcaaaatac cttgcagacg tatgaaaacc atttacaagc
     9301 ggtctaattt tacggatatt ttgcaaatcc tcggcattgg tcgaggattt ctttttctgg
     9361 gctttttcag cagaaaaggc taaaatactt gccattttta ttacacgatc cttattaaat
     9421 atgaaattta tcatcaaact tttccctgaa attatgatta aaagcgactc tgttcggaaa
     9481 cgctttatca aaattttaac ctcaaatatt cgcaacgtat tattaaaaga gactgaaaat
     9541 gtgtctgtgg ttcgccattg ggactttatt gaagttcggg cgaaagtgga cgaagaaacg
     9601 gcattagtgc tggatttgct taaacgcacg ccgggtattc accatatttt agaagtggag
     9661 gaaacccctt ttacggatat gcacgatatt tttgagcaga cttatgccgc agtgaaagct
     9721 gaactagaag gcaaaacgtt tagcgtgcgg gttcgccgta aaggaaagca cgatttccgt
     9781 tctcttgatg tagagcgtta tgtcggtggt gggctgaatc agcggattga aacggcgaaa
     9841 gtgaaattaa ccaacccaga tgttacggtc agaattgata ttgaacatga taaaatgttg
     9901 ctgattaaag ctcgccacga agggttaggc gggtatccaa tcggcacaca agaagatgtg
     9961 ttgtcattga tttcgggtgg ttttgattcg ggtgtatcga gctatatgct gattcgcaga
    10021 ggttctcgtg tgcattactg ttttttcaat ttaggtggtg ccgcacacga aattggcgtg
    10081 aaacagatgg catatcatat ttggagccgt tatagtacct cgcacaaagt ccgttttatc
    10141 gccattaatt ttgaaaatgt agtgggtgag attttagaaa aagttgataa cggacaaatg
    10201 ggcgtggtat taaaacgtat gatggtgcga gcggcaagcc gtattgccga gcgttttgat
    10261 attcaagcga ttgtgaccgg tgaggcgttg gggcaggtct ctagccaaac actgactaat
    10321 ttacgtttaa ttgataaggc atcagatacg ctggtattac gtccgctcat cactcacgat
    10381 aaagagcaga ttattgcaat ggcaaaagcg ataggcactg acgatattgc taaatcaatg
    10441 ccggagttct gtggagtaat ttctaaaaat ccgacggtta aggcaatcga aactaaaatt
    10501 ttggaagaag aaggcaactt taattttgag gtactagaac aagcggttca aacggcgagt
    10561 tatttagata ttcgtgagat cgctcagcaa accgaaaaag aggttgtttc ggttgaaacc
    10621 acaacagaat tgagtaaaaa cgatgttgtg ctagatattc gcagcccgga agaggttgat
    10681 gaaaaaccat tccgtttaga cggggttgaa gtaaaagaac tcccgtttta taaactttcc
    10741 agccagttta gtacgcttga ccaaagcaaa acctacttgc tctattgcca acgtggggta
    10801 atgagtaaac tgcaggctct ttatttgaaa gagaatggct acagcaatgt aaaagtgttt
    10861 cgagtgaaat aatgcctctt tgattgcacc ttaatgattg attaaggtgc aattttttac
    10921 caaataaggc ttgctaaaaa gagtaagatc acaggaaatt caagcattcc aacttgttta
    10981 acattccagc caaagcttgg cacaataatg gctcgggcag ttgcaattaa ataagcaaaa
    11041 aagaccgctt gtagagagca gagtatgtaa agcattgcta aaaccaaatg tattgcaata
    11101 ctgatttcca tataaattgg gtttcttcgc tctcgcacca tactctttat atagaaagtc
    11161 gtagcaataa aaaatagggt agggtggagc agaatctcaa actgatattg tttcgttacc
    11221 agataataag ctgccattcc cattatgcca aaggttagaa tgccggcaat atcattccat
    11281 aaattgcgtt catctttttg tttggcgtag tgaacttgca ctaacccaag cggtagaatt
    11341 atcggtaaaa attgcaaaat cgaaggctga gcgtagatca aaggtaatgc aaatagaatg
    11401 cttaagccaa agtagatttt cgcccatttt ttattgcgtt cagttggctt tttactgaaa
    11461 agtgagagaa aagggtagga aaagaagtag agaaacagcc acgacagccc aaagaaaata
    11521 tgcaaagtgc taaaaccact ttcaataacg gcgtagagaa acggcaacat tgccattgct
    11581 aatgcaccgt gttggttgga tataacgggt ttttctttga acattcaatt tccctcttaa
    11641 tgtggcaaat aaaaaggcga ctaaacagcc gcctttaaat tgtaagaaaa gttagaataa
    11701 ttttctagag gcttcaaata attcttcttt gaacggacga cgcatattat tgatcgcatc
    11761 aataatatcg tggtgaacca actgctcgtt ttgaataccg acacagtgac cgccaaagcc
    11821 ttgcagtaat aattccaccg catagacgcc catacgggag gccaaaatac ggtcgaaggc
    11881 acaaggcgaa ccaccacgtt gaatatgacc taatacggtt gcacgggttt cattgccgaa
    11941 acgtgcttca atttctttcg ctaatgcgtg aacatcggtc atcaattctg taattgcaat
    12001 aattgaatgg cgtttacctt tgtggaaacc ttcttcaatg cttcgcatta atgcatcttt
    12061 atctaagcct ttttccggta cgatcacata ttcgcaacca ccggcgaggg cagcacttag
    12121 ggttaaatca ccacagtggc gacccataat ttccacaatc gaaatacgtt ggtgtgaact
    12181 tgaagtatca cgcaggcggt caatcgcatc taatgcggtt tcaagggcag tttgatagcc
    12241 gatggtgtaa tccgttccca caatatcgtt atcaatcgta cccggaatac cgatacaagg
    12301 gtaaccaaat tcttcggtta ataatttagc ccccatataa gaaccgtcgc caccaataac
    12361 cactaatgca tcaatgctgt atttttttaa ggtttcgaca caatgtttgc gaacttctgg
    12421 tcttttaaaa tcagggaagc gggcagaacc taagaatgta ccgcctcggt tgatggtttc
    12481 agacacggaa cggcggtcaa gtgggatcac tttatcgtgg taaagcccat agtagccgtc
    12541 ttgaatacca caaacctcta aaccttcatt aagtgcggta cgaaccacac cacggattgc
    12601 cgcgttcatt cctggtgcat caccaccact ggttaatact gcaatttttt tgattttttt
    12661 actcatttaa aaacaccttg ttgataaatt aaagttaaat tggaaactgg tgcaaggtta
    12721 caatattcag ccttttcgtt ctactaaaaa atacgtattt ttttgattta gcttagcttt
    12781 cttgcaaaat ttcaagggta aaatctagag cgttgaaaaa atgctcaaca tcttgttgat
    12841 tattgtaatg ggcaagtgaa agtcgaactg ttccgacctc ttctaaatgg cgtaaataag
    12901 gcttggcaca atgctcccct tggcggatcg caattttact ttgggttaag aaagcggcaa
    12961 tatcggcagg gtgctgattt ttcaccacaa agctaatagt cggtgtattt tgttgcccga
    13021 taatttgcaa attttgataa gaatttaacc gcttgcaggt gtattcagcc aagtcataaa
    13081 gctgatgatt aagagcgaca aaatcccatt tttcgagcca agctagcact tgcccaaagc
    13141 cgataatgcc ggcgatattc ggcgtgccag cttccaagcg ataaggtaac tctgccacag
    13201 ttaattcact ttccgtaatg tttttcagca ttttgccgcc aaatacaagc ggttgaattt
    13261 gctccaaact ttgcaatttg ccggtaagca cgcccacacc gtttggacca tacattttat
    13321 gggcggaaaa agcgtagaaa tctgcatcta atttctgaac gtccacttgc tctgagcaaa
    13381 ctgcttgagc aatatctaac acaattttag cagagcaaga ttggcggata agcgggatta
    13441 tttgttccgc tggctggcga actcctgtta cgttagaaac taaattaaag gcaacaattt
    13501 tggttttttc cgataacgca tttttaaggg tttcaagctc aatttcatac ttatcattaa
    13561 gtggcaacac cacgagtttc gcccttgtac gctgtgccaa ctgctgccaa ggaataaaat
    13621 tagcgtgatg ttccgccact gaaattacaa tttcatcgcc tgctgcaatt tgatgctcca
    13681 agccataagc cacggtgtta atactttggg ttgtaccgct tgtccaaatc accgcttcac
    13741 gagtttccac attgaaacgt tttgcgacta aatctcgagc taattcatat tgttcggttt
    13801 gtggcaaatc gtactgactg cgatgcaccg accccgcaga agcgtaaaac tcacttgttg
    13861 cttcaatgag tacatcaggt ttcagcgttg tagcggcaga atctaaatac gtccatccgt
    13921 cagatttttc aaaaaaaggg aaatgggtgc gaaattgggt gttaagcata agttaattcc
    13981 taagagttgt ggaaaattga gttaccaaga aaatacctat tataaaggaa tgttttgagg
    14041 taagaagcat ttcaagaaat aaatagattt attttgttat ctttttgtta aattcgtgtt
    14101 gactaataaa attattttta taaa
//
LOCUS       DANLRK010000055        14050 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.055, whole genome shotgun sequence.
ACCESSION   DANLRK010000055 DANLRK010000000
VERSION     DANLRK010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 14050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14050
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.055"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(21..866)
                     /gene="nfo"
                     /locus_tag="PXA33_001161"
     CDS             complement(21..866)
                     /gene="nfo"
                     /locus_tag="PXA33_001161"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HDL5120497.1"
                     /translation="MKYIGAHVSASGGVENAVLRSVEIGANAFALFTKNQRQWQAPAL
                     KAETIEKFKRFCKVHHFSAEQILPHDSYLINLGNPEAEALEKSRVAFIDEMSRANQLG
                     LKLLNFHPGAHLKQISEQDCLARIAESINIAVEKVPNVIAVIENTAGQGSNLGWHFDH
                     LATIIEQVEDKSRVGVCLDTCHLFSAGYDISSYEKSEAVFNDFNQTVGFHYLRGMHLN
                     GSKTPLGSRVDRHHTLREGTIGTEVFAFIMQNMHFNHIPMILETISPEIWADEIKFLR
                     TLELK"
     gene            925..1494
                     /locus_tag="PXA33_001162"
     CDS             925..1494
                     /locus_tag="PXA33_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HDL5120498.1"
                     /translation="MNETTINDFSLLCRLFGNLFYRHPTDPILAGTFNWLAQGGLRQV
                     WALNTDSQSENALTLLQKNANPTQLAASYQALFAENGAISPTISAYGLSVNNFVAFRQ
                     ERAMPELENADHIGLLFLTASWIEDNLDSALAQKILFEQFLLPCAGKFLGKIEAFDNG
                     FYKALAQLSRDALSAMADELEESELEAVE"
     gene            1524..1907
                     /locus_tag="PXA33_001163"
     CDS             1524..1907
                     /locus_tag="PXA33_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5120499.1"
                     /translation="MKTLKLGCIFSLALMLSSCTLTPEQQAERKAKQIRAEQDLQVQL
                     AKQCDVETAELMYQQFNPPLSQTEQEEKAFKKRYAEKVNAPMFQACYKLAWQNYKAQV
                     ELEEIRWNYDRDYMYRGWRYCYYCW"
     gene            complement(1988..2596)
                     /locus_tag="PXA33_001164"
     CDS             complement(1988..2596)
                     /locus_tag="PXA33_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HDL5120500.1"
                     /translation="MKLTKIALSLTALVVSSMSVAGTLSTSSMVDILAFDGQRVKSGT
                     TSVQMSENKTHQVVVEVGGTVEGDFFTSDQIILTFQGSAENAKLETPRLNSKLDLRKF
                     QENQTVAIKTDSGKVISHKQDFLKGEGFLAKTRVEDNLTKYNLAKKTASVEQFATAPL
                     EAKGQIVVDTNKVSQEELQLLFRKADKDTQKRFLEWAKKNIK"
     gene            2734..3633
                     /gene="corC"
                     /locus_tag="PXA33_001165"
     CDS             2734..3633
                     /gene="corC"
                     /locus_tag="PXA33_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249295.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HDL5120501.1"
                     /translation="MSDEQQSVNTTEKSEKKSILHSLFSGLFQNEPKNREDLVEVIRD
                     SVENELIDSDTKEMIEGVMEISELRVRDIMIPRAQIVFIDANEPLDVCIDLIIESAHS
                     RFPVIRGEKDTIEGILHSKDLLKYLRSDSEPFNLQQVLRPAVIVPESKRLDRMLKEFR
                     SERFHMAVVVDEFGAVSGLVTIEDILEQIVGDIEDEFDEEEVKPIRQLSRHTYAVLPL
                     TDIKKFNEFFATDFNDEEVDTIGGLVMQAFGYFPKRDEQIELNGIDFKVTSANSRQLI
                     QLRVTLSDEQLEKMEKTSPNEEE"
     gene            3692..5200
                     /gene="lnt"
                     /locus_tag="PXA33_001166"
     CDS             3692..5200
                     /gene="lnt"
                     /locus_tag="PXA33_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005611523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HDL5120502.1"
                     /translation="MNFAKNPPFLTACLLAIILGAIGTFAYSPFDIWILAFASAAGLV
                     WSATLANRKTALWATFAWSIGYFCTGVNWVSVSMTQFGGVPTIVSYIAVFLLACYLAI
                     YNLLFSVVSHRLKLNNPFALAAIFTFTEYLRGVVFTGFPWLQFGYSLIDSPFAGIAPI
                     LGVEGLTFLVIVASSYLVKIIQQENKTVPSLMVLAVIFGLSFATRFLQFVQIDTEKQP
                     LVVSLVQGNIEQKMKWDPQHFNHSVRTYEQLILPLLGNSELIILPESAIPALENQISP
                     LLGSLDHLARQHGSEIIIGTLYQNEQEELFNSSVLLGEKEREYALHSNPRYNKHHLVP
                     FGEYVPFGSVLDWMREVFILPINLSQGSFVQPSFLAKNNRFNMAICYEIIFGDQLQQN
                     QKSQNSDYLLTITNDAWFGSSIGPWQHFQMARMRALELGKPLLRAANTGITAIVDANG
                     KVIEQLPQFTADALTATIVPTKGDTPFKQLGHWLIYSFSLFCVIASLLLRRK"
     gene            complement(5246..5710)
                     /locus_tag="PXA33_001167"
     CDS             complement(5246..5710)
                     /locus_tag="PXA33_001167"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HDL5120503.1"
                     /translation="MATFQGNYIATGLKFGIVATNWHKIFIDQLLNGATDKLLRHGVE
                     EKNIDTVWVPGALEISIAAKKMAQSGRYDAIICLGAVVRGATSHYDVVVNESAKGISS
                     SALETGVPIINGILTVENLEQAIERSGTKAGNKGEECAMVAIEMANLLKALA"
     gene            complement(5916..7115)
                     /locus_tag="PXA33_001168"
     CDS             complement(5916..7115)
                     /locus_tag="PXA33_001168"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HDL5120504.1"
                     /translation="MFKFSTVEEAIQAIAQGKIILVSDDADRENEGDFICAAEFATPE
                     NINFMAKYGKGLICTPISTEIAKKLNFHPMVLENEDNHQTAFTVSVDHIQTGTGISAF
                     ERSLTCLKMLDENAKPEDFRRPGHVFPLVAKDGGVLVRNGHTEATVDLARLAGLKPAG
                     LCCEIMAEDGTMMQMPELQAFAQAHNMPFITIQQLQEYRRKHDSLVNVVSVVKMPTKY
                     GEFKAHSFVETISGKEHVALVKGDIGDGENVLCRIHSECLTGDAFGSQRCDCGQQFAA
                     AMNQVEQEGRGVVLYLRQEGRGIGLINKLRAYELQDRGMDTVEANLALGFKDDEREYY
                     IAAQMFEKLGVKSIRLLTNNPAKIEGLIEQGLNVVAREPIMVEPNEHDLEYLKVKQHK
                     MRHMFNF"
     gene            complement(7134..7781)
                     /gene="ribE"
                     /locus_tag="PXA33_001169"
     CDS             complement(7134..7781)
                     /gene="ribE"
                     /locus_tag="PXA33_001169"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HDL5120505.1"
                     /translation="MFTGIIEEVGQIAQIKKQGEFVVVTVKAKKILTDVHLGDSIAVN
                     GVCLTVTSFTQEQFTADVMSETLKRTSLGELSINSSVNLERAMAANGRFGGHIVSGHI
                     DGTGVVAEITPADNSTWYRIKAEPKLMRYIIEKGSITIDGISLTVVDVDAESFRVSII
                     PHTIKETDLGTKKIGSLVNLENDIVGKYIEQFLLKKEPENPQSKITADFLKNAGF"
     gene            complement(7998..9095)
                     /gene="ribD"
                     /locus_tag="PXA33_001170"
     CDS             complement(7998..9095)
                     /gene="ribD"
                     /locus_tag="PXA33_001170"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005708727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HDL5120506.1"
                     /translation="MTDVQYMAYAITLAEKARGWTSPNPLVGCVIVKNGNIIAEGYHQ
                     KAGSWHAERNAILNCEEDLTGATAYVTLEPCCHHGRTPPCSELLIERGIKKVFIGSRD
                     PNPLVSGKGAAQLRAAGIEVVEDFMRTECDALNPIFFHYIQTKRPYVLLKYGMTADGK
                     IATYTGNSKWITGEAARANVQKSRHEYSAIMVGVETVLADDPMLNSRMENAKQPVRIV
                     CDSQLRTPLDSQLVQTARQYRTIIATLNNDAKKQAEYTACGVEILVTGERDKRVDLAD
                     LLQKLGELGIDSLLIEGGSQLNFSVLEQGLVNRVHCYIAPKLIGGKAAKTPIGGKGIG
                     ELAQAVKLRLKSTELLGEDILLDYEVDLLRN"
     regulatory      complement(9271..9389)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            9593..11995
                     /gene="lon"
                     /locus_tag="PXA33_001171"
     CDS             9593..11995
                     /gene="lon"
                     /locus_tag="PXA33_001171"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HDL5120507.1"
                     /translation="MAPRKKKAIELPLLPLRDVVVFPYMVMPLFVGREKSIQALRSAM
                     DSNKQLFLVTQQDPNKEEPNAEDMYGVGVIANIIQMLNLPDGTVKVLVEGQTRAKIEQ
                     IHDDENGFWAAIQPIYSEYDDENEELKAIAKTTLTEFENYVKNNKKIPAEIIAKLQKI
                     TLEDRLADTIASNLIAPVKKKQELLEQPNLIARFEALLIAMATEMDTLETETRIRNRV
                     KQQMEKNQRDYYLNEQIKAIQKELGNGDDVEQSELDKLKEKIDAAKLPTEVKDKLNTE
                     FKKLKAMPQSSSEATVVRGYIDWILQMPWHKKSAVKKDLAQAQEILDKDHYGLERVKE
                     RIVEYLAVQSRLNKLKGPILCLVGPPGVGKTSLGQSIANATGRKYVRMALGGVRDEAE
                     IRGHRRTYIGSMPGSLMMKMAKVGVRNPLFLLDEIDKMAQDMRGDPASALLEVLDPEQ
                     NKAFNDHYLEVDYDLSDVMFVATSNSMNIPPALLDRMEVIRLSGYTEDEKMHIARGHL
                     LAKQQENNGLKEGELTIEDSAILSIIRYYTREAGVRSLEREIAKICRKAVKALVLDKK
                     LKSITVSEKNIEEYLGVKRFDYGKMDSQNRIGEVTGLAWTEVGGDLLTIETASVAGKG
                     KFSYTGSLGDVMKESIQAAMVVVRSRAEKLGIAEDFHEKRDIHVHVPDGATPKDGPSA
                     GIAMCTALISSLTGNPVRKEVAMTGEISLRGKVLPIGGLKEKLLAAHRGGITTVIIPK
                     ENEKDLEEIPENAKAALDIHAVETIDEVLALALENPPMGIEILSKAEIKVKKSRAKAT
                     VQ"
     gene            complement(12129..13019)
                     /gene="ilvY"
                     /locus_tag="PXA33_001172"
     CDS             complement(12129..13019)
                     /gene="ilvY"
                     /locus_tag="PXA33_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HDL5120508.1"
                     /translation="MDFQSLKLFLDIAQSRSFIRSAEKNYMTASTLTRHIQRIEEELG
                     QPLFLRDNRQVRLTEAGERFLAFAQQGWAEWQQLQNQLSPAQGDLEGELKVFCSVTAS
                     YSHLPPILGKFRQKYPKVDIKLSTGDPALALEQVQSLAADISVAGKPEFLPNNIVFHY
                     IDDIQLSIIAPRVASQATQFLQQSPIDWKNIPFILPVNGPVRKRIDRWFKEQKIKDPI
                     IYATVSGHEAIVPMVALGFGVALLPDIVIKHSPMNNQVSYVNLPSPITPFELGICVQQ
                     KRLQEPIINAFWQLLSDNSA"
     gene            complement(13021..14001)
                     /locus_tag="PXA33_001173"
     CDS             complement(13021..14001)
                     /locus_tag="PXA33_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005619717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HDL5120509.1"
                     /translation="MLDSKNTDLILSEIPNKILFLRHDGKIGDYVVSSFVFREIKKQS
                     PQTQIGVVCSQKNRYLFENNPYIDKLYLVKTKSIPDYIRGGKQLAKEQYDVVIDPTVT
                     LRNRDLLFLRTISAKHYVGYQKQKYRLFDLNVEDRKQHFSEIYQDALALIGFENIETQ
                     YDVPLNATSNQSIKHYLYENQIRNYIALNLFGAGSARRFSEQKINELLSCLKQHSDKP
                     IVLLTFPEVTAKLCSFTKKYKNIFVYEKTKSVFDSIELIRFADLLISPDTATVHIASG
                     LSKKIIAFYSSDEQNFIHWHPNNQAETHILRFRKSVNDLDFAAIKPEWLK"
BASE COUNT         4093 a         3020 c         2762 g         4175 t
ORIGIN      
        1 actactcaga ccagtataac ctattttagt tccaatgtgc gtagaaattt tatttcatct
       61 gcccaaattt caggcgaaat tgtctctaaa atcataggaa tatggttaaa atgcatattt
      121 tgcataataa atgcaaacac ttcggttcct attgtgcctt ctcttaaggt atgatgacga
      181 tccactctgc tgcctaatgg tgtttttgaa ccatttaagt gcattcccct taaataatga
      241 aaaccaaccg tttggttaaa atcattaaat actgcctcgc tcttttcata agaactaata
      301 tcatagccgg cagaaaataa gtgacaagta tctaaacaca cccccactcg gcttttatcc
      361 tcaacttgct caataatagt tgccaaatga tcgaaatgcc aacctaaatt agaaccctgt
      421 cctgccgtat tttcaatcac ggcaataaca ttcggtactt tttcaactgc aatattgatc
      481 gattctgcaa ttctagccaa acaatcttgc tcggaaattt gctttaaatg cgcccccggg
      541 tgaaaattca acaattttaa acctaattga tttgctcgag acatctcatc aataaatgct
      601 acacgagatt tctccaatgc ctccgcttcc ggatttccta aattaatcaa atagctatcg
      661 tgtggtaaga tttgctctgc tgaaaaatga tgcactttac aaaaacgctt aaatttttcg
      721 atagtttcgg cttttaatgc cggtgcctgc cattgacgtt gattcttagt aaataacgca
      781 aatgcattag caccaatttc tacggaacgc aaaactgcat tttccacccc acctgaagca
      841 ctaacgtgtg cgcctatata tttcattcag tcctctcttg tgttctgcta tactaacaat
      901 tcttttttct atctatttca aattatgaac gaaactacca ttaacgattt ttccctgctt
      961 tgccgtttgt ttggcaactt attttatcgt caccctaccg accctatttt agctggcaca
     1021 tttaactggc ttgcccaagg aggattacgc caagtctggg cattaaatac ggatagccaa
     1081 agtgaaaatg cgctgactct cttacaaaag aatgctaacc ctacgcaatt agcagcaagc
     1141 tatcaagcgc tttttgctga aaatggggct atttctccaa caatttcagc ctatggatta
     1201 tcagttaata attttgtagc ttttagacaa gagcgggcaa tgcctgaatt agaaaatgcc
     1261 gatcacattg ggttactatt tctcacggca tcttggatcg aagataatct cgattctgcc
     1321 ttagctcaaa aaatattatt cgaacaattt ttactacctt gtgcaggcaa attcttagga
     1381 aaaatagaag ctttcgacaa tggtttttat aaagccctcg cacaacttag ccgtgatgcc
     1441 cttagtgcta tggctgatga acttgaagaa agtgagttag aagcggtaga ataaatcacc
     1501 aatttcaact agaggattca gcaatgaaaa cactcaaatt aggctgcatt ttttcactcg
     1561 cacttatgtt aagtagctgt actttaactc cagagcaaca agctgaacgt aaagcgaagc
     1621 aaattcgggc agaacaagat ttgcaagttc agctcgccaa acaatgtgat gtagaaacgg
     1681 cggagttaat gtaccaacaa tttaatccgc cgcttagcca aaccgaacaa gaagagaaag
     1741 catttaagaa acgctatgcc gaaaaagtca atgccccaat gttccaagcc tgttataagc
     1801 ttgcttggca aaattacaag gcacaagtag aacttgaaga aattcgttgg aattacgata
     1861 gagactatat gtatagaggc tggcgttact gctattattg ctggtaaatc tcattgtatg
     1921 agcggtcatt tttttaacac cttgcaaaaa attggcgaga aatgaccgct tattaattga
     1981 tataggttta ttttatattt ttcttcgccc attctaagaa acgtttttgg gtgtctttat
     2041 ctgcctttct aaataaaagc tgtaattctt cttgtgaaac tttatttgta tccaccacaa
     2101 tttgtccttt tgcttcaaga ggtgccgtcg caaactgttc aactgaagct gtttttttcg
     2161 ctaagttata tttcgttaaa ttatcttcaa ctcttgtttt cgcaaggaaa ccttcccctt
     2221 ttaagaaatc ttgtttatgt gaaataacct tacctgaatc ggttttaatt gccacagtct
     2281 gattctcttg gaatttacga agatctaact ttgaatttaa tcttggcgtt tctaattttg
     2341 cattttccgc actgccttgg aaagtcaaaa tgatttgatc agaggtgaaa aaatcccctt
     2401 caactgtccc accgacttcc actaccactt gatgcgtttt attctcactc atttgaaccg
     2461 aagtcgtgcc ggatttaaca cgttgcccgt caaaggctaa aatgtccacc attgatgatg
     2521 tacttaacgt cccggccaca ctcatcgaac tgacaactaa ggctgttaaa cttaatgcta
     2581 ttttggttaa tttcatttca aactcctaaa atatgaagaa ataaaaagat ttttgcttat
     2641 cctatgctaa tttgagtgaa aatagcaatg ttttatttgc tttgtgactg aatttgctta
     2701 ttataagcgt actttattta agtggaacaa actatgagtg atgaacagca aagtgtaaac
     2761 accaccgaaa aatcagaaaa aaaatcgatt ttacactctc tatttagcgg gctttttcaa
     2821 aacgagccta aaaaccgtga agatctagtc gaagtgattc gagactctgt tgaaaatgaa
     2881 ttgatcgaca gcgacaccaa agagatgatc gaaggagtaa tggaaatctc ggaattacga
     2941 gtgcgggata ttatgattcc ccgtgcgcaa attgtgttta ttgatgccaa tgaaccttta
     3001 gatgtctgta ttgatttaat tattgaatcc gcacactctc gttttccggt gattcggggt
     3061 gagaaagata ctatcgaagg tattcttcac tctaaagatt tattgaaata tttacgttct
     3121 gactctgaac cctttaatct gcaacaggta ttacgccctg cggttattgt accggaaagc
     3181 aaacgcctag accgaatgct aaaagagttc cgttcagaac gcttccatat ggcagtagtg
     3241 gttgatgaat ttggtgcggt ttcaggctta gtcaccattg aagacattct tgaacaaatt
     3301 gtgggtgata ttgaagatga gtttgatgaa gaagaggtca aaccaattcg ccagctttca
     3361 agacatactt atgcggtatt accgctcacc gacatcaaaa aattcaatga attttttgct
     3421 accgacttta atgacgaaga ggtggatacc attggtggtt tagtgatgca ggctttcggt
     3481 tatttcccga aacgagatga gcaaattgag ttaaacggaa ttgattttaa agtcacctca
     3541 gctaatagcc gccaattgat tcagctacga gtcacacttt ccgatgaaca gttagagaaa
     3601 atggaaaaaa cctctcctaa cgaagaagaa taatatagtg agtttcaatc aaggcacgtt
     3661 tcggcgtgcc tcatttattt agtgagttat tatgaatttt gcaaaaaatc cgcctttttt
     3721 aaccgcttgt ctccttgcaa tcatactggg tgcaatcggc actttcgcct attcgccttt
     3781 tgatatttgg attcttgcct ttgcttccgc agccggcttg gtttggtcag ccactctggc
     3841 aaacagaaaa accgcattat gggcaacctt tgcttggtct atcggctact tctgcaccgg
     3901 tgtaaactgg gtttctgtga gtatgaccca atttggtggt gttcctacca tagtaagcta
     3961 catagcagta ttcctgttgg cgtgctatct cgctatttat aatctgctgt ttagtgtagt
     4021 ttcacacagg ctcaaactga ataatccgtt tgcattggct gcgattttta cctttaccga
     4081 atatttacga ggcgtggtat ttaccggttt tccgtggcta caattcggct atagcttaat
     4141 agacagcccc tttgctggta ttgcgccgat tttgggcgtt gaaggtttga ctttcttggt
     4201 aatagttgca agtagctatc tagtgaaaat tattcagcaa gagaacaaaa ctgtgccatc
     4261 attgatggta ctagcggtca tttttggctt aagttttgcc actcgctttc tccaatttgt
     4321 gcaaattgat acagaaaaac aaccgcttgt agtgagttta gtacaaggga atattgagca
     4381 aaaaatgaag tgggatccgc aacatttcaa ccacagcgta cgaacctacg aacagctcat
     4441 tctgccccta ttaggtaaca gtgaactgat tattttgcct gaatccgcca ttccggcact
     4501 tgaaaatcaa atcagcccgc tgcttggctc gctcgatcac ttagctcgcc aacacggaag
     4561 tgaaattatt atcggcacac tctatcaaaa tgagcaagag gaactgttta acagtagcgt
     4621 gctattaggc gaaaaagaga gggaatatgc cctgcacagc aaccctcgct acaacaaaca
     4681 tcaccttgtg ccgtttggcg aatatgtgcc gtttggctcg gtgctggatt ggatgcggga
     4741 agtgtttatt ctaccaatta atctttcaca aggcagtttc gttcaaccat cattcttggc
     4801 gaaaaacaac cgatttaata tggcgatttg ctatgaaatt atttttggtg atcagttaca
     4861 acaaaaccaa aaaagccaaa attcagatta tttattaact atcaccaatg atgcgtggtt
     4921 tggttcttcc atcgggcctt ggcaacattt ccaaatggca agaatgcgag cgttagaact
     4981 tggcaaacca ttgcttcgag ctgcgaatac cggtattacc gccattgttg atgccaacgg
     5041 aaaagtaatt gaacaactac cgcaatttac tgctgatgca ctaactgcca ccattgtgcc
     5101 aaccaaaggc gatacgccgt ttaaacaatt aggtcactgg ttaatttaca gtttcagcct
     5161 attttgtgtc attgccagcc tgttactacg cagaaaataa aaaaatcccc ttaggctctt
     5221 gggtctaagg ggattttatc gctgattacg ctaaggcttt taataggttc gccatttcaa
     5281 tcgctaccat tgcacactct tcgcctttat taccggcttt agtgcctgaa cgctcaatcg
     5341 cttgctctaa attttccacc gttaaaatac cgttaataat cggcacgccg gtttctaaag
     5401 cagaagagct gatgcctttt gctgactcgt tcactaccac atcatagtgg ctggttgctc
     5461 cacgcacgac cgcacctaag cagataatcg catcataacg accgctttgt gccatttttt
     5521 tcgctgcaat tgagatttct aatgcacccg gcacccatac ggtgtcgata tttttttctt
     5581 ctacgccgtg acgcaataat ttatcggttg caccgtttaa cagttgatcg ataaaaatct
     5641 tgtgccagtt agttgccaca ataccgaatt ttaaaccagt tgcgatgtag tttccttgaa
     5701 atgttgccat tgtttgtttt ccttctttaa aataaatttg atactcaata accctctccc
     5761 cttgtgggag agggatagat ttttcttcgt tcagaagaaa aatcagggag aggggaaatt
     5821 gcaaactttt tctaaaaaat ataccctctc tctgaaaaat acttctgaac gaagtatttt
     5881 tctgtctctc tcccacaagg ggagagagta ttggattaga aattaaacat atgtcgcatt
     5941 ttgtgctgtt taactttaag gtattccaaa tcgtgttcgt ttggctcaac cataatcggc
     6001 tcacgagcca ctacgtttaa accttgctca atgagtcctt caatttttgc cggattattg
     6061 gttaacaaac gaatcgattt tacgcctaat ttttcaaaca tttgagcggc gatataatat
     6121 tcacgctcat catctttgaa acctaatgcc aggttggctt caaccgtgtc cattcctcta
     6181 tcttgcaatt cgtaagcacg cagtttatta attaaaccga tgccacgtcc ttcttggcgt
     6241 aaataaagca ccacgcctct gccctcttgc tccacttggt tcattgccgc tgcgaactgc
     6301 tgaccacaat cgcaacgttg tgagccaaag gcatcgccgg ttaaacattc ggaatggata
     6361 cggcataata cattttcgcc atcaccaata tcacctttaa ctaaagcgac gtgttctttg
     6421 cctgaaatag tttcaacgaa actgtgtgct ttgaactcac cgtatttggt cggcattttt
     6481 accaccgaaa ccacattcac caagctgtcg tgttttctac gatactcttg taactgctga
     6541 atagtaataa acggcatatt gtgtgcttgt gcaaatgcct gtaattccgg catctgcatc
     6601 atcgtgccgt cttctgccat aatttcacag caaagccctg ccggtttcaa gcccgccaaa
     6661 cgagctaaat ccacagttgc ttcggtatga ccgttacgca ctaatacgcc tccgtctttt
     6721 gccactaatg ggaaaacatg ccccggacgt ctaaaatctt ccggcttagc attttcatca
     6781 agcattttca ggcaagttaa tgaacgctca aaagcggaaa taccggtgcc ggtttgaata
     6841 tggtcaacag aaacggtaaa tgcggtttga tgattatctt cgttctctag caccatcggg
     6901 tgaaaattta attttttggc aatttctgtc gaaatcggcg tacaaatcaa ccctttacca
     6961 tatttcgcca taaagttgat attttccggt gtcgcaaatt ccgctgcaca aatgaagtcc
     7021 ccttcatttt cacggtcggc atcatcgctg actaaaataa ttttcccttg tgcaattgct
     7081 tgaattgctt cttctaccgt tgaaaactta aacattattt gtcctttatt tggttaaaac
     7141 cctgcatttt taagaaagtc cgcagtaatt ttactttgcg gattttcagg ctcttttttc
     7201 aataaaaact gttcgatata tttgccgaca atgtcatttt ctaaattcac caaactaccg
     7261 atttttttcg taccgaggtc ggtttcctta atggtatgcg gaataatcga tacccggaaa
     7321 gattccgcat caacatccac caccgttaaa ctaatgccat caatagtaat tgagcctttt
     7381 tcaatgatgt aacgcattaa cttaggttcg gctttaatgc gataccaagt tgaattatct
     7441 gctggtgtaa tctctgcaac cacacccgtg ccatcaatat gacctgacac aatatgccca
     7501 ccaaaacgcc cgtttgccgc cattgcccgc tcaagattaa ccgagctgtt gatacttaat
     7561 tcgccaagtg atgtacgttt taaggtttct gacataacat cagcagtaaa ttgctcctgc
     7621 gtaaatgaag tcacggttaa acacacgcca tttaccgcaa tgctatcccc taaatgtaca
     7681 tcggtcagaa tcttttttgc tttaaccgtt accaccacaa actcgccttg tttcttaatt
     7741 tgggctatct ggccaacttc ttcgataatg cctgtgaaca tttaaatttt tctccgattt
     7801 acttccaatt tagtctgaga actgcttatc ttactattcc cctctcccgt ttacgggaga
     7861 gggaagagaa aatccgtagg attttcggag ggagagggaa gattacactc attttcccct
     7921 ctctctgcat aaaatttctg aacgaaattt tatgctgtct ctctcccgta aacgggcgag
     7981 agtaaggaaa tttcaattta attacgaagc aagtccactt cataatccaa caaaatatct
     8041 tctccgagca attcggtgga ttttagacgt aatttaaccg cttgtgctaa ctcgccaatc
     8101 cccttgccac caatcggggt ttttgccgct ttgccgccaa tcagtttcgg ggcaatatag
     8161 caatgtactc gattgactaa cccctgttcc agcacgctga aattgagttg cgaaccacct
     8221 tcaatcaata agctgtcgat cccgagttcg cccagctttt gcaaaagatc ggctaaatcc
     8281 acccgcttgt cacgttcgcc tgtcactaaa atttcaacac cgcaagcggt atattctgcc
     8341 tgcttttttg catcattatt gagcgtagca ataatggtgc gatattggcg tgcagtttgg
     8401 actagttggc tatccaatgg ggtacgcagt tggctgtcac atacaatccg caccggctgt
     8461 ttagcatttt ccattcggct gttgagcatt gggtcatcgg caagcaccgt ttccaccccg
     8521 accataatcg cactatattc gtggcggctt ttctgtacat tagctcgggc agcctcgccg
     8581 gtgatccatt tcgaattccc cgtataagtc gcaattttac catctgccgt catcccatat
     8641 ttcagcaaca cataagggcg tttggtttga atgtaatgga agaaaatcgg gttcagcgca
     8701 tcgcactccg ttcgcataaa gtcttccaca acctcaatgc cggcggctct aagctgtgcc
     8761 gctcctttgc cagaaacaag tgggttcgga tctcgagagc cgataaacac ttttttgatc
     8821 ccacgttcaa tcagcagttc ggaacaaggc ggggttcgcc cgtggtggca acaaggctct
     8881 aaggttacat aagcggttgc ccccgtcaga tcttcctcgc agtttaaaat cgcattgcgt
     8941 tcagcgtgcc aactaccggc tttttggtga tacccttcgg cgataatgtt gccgtttttc
     9001 acgatcacac agccaactag cggattaggg cttgtccaac cccgtgcttt ttcggcaagg
     9061 gtgatggcat aagccatata ttgaacatct gtcatataca ttttgataaa ttgagggcgt
     9121 aatgaaagga ttattgcaat acaagcggtc gtttttagta aaaaatttgc aaacgccgcc
     9181 tgcaaaacaa tgaagccttg aaatctcacg actcaaggct tgcaaaaaag acacaaaaaa
     9241 tgaccgcttg tgcggtattt tttgcggttt ttgactcttc tttcatccag actatactgt
     9301 cggttttgga atttcaccaa atcctgccga ttgctcggct cgcggacttt accgccgatc
     9361 gggaatttca ccctgccctg aagattaaaa tatgcagggg attttgctcg cattacgatt
     9421 tgatgtcaag cgggaaatta agctaattac cctgatttta acaattgatt aaatcgccaa
     9481 catctactgt tttgactatt cgagtttttt taagataaat caaattttcc cttgtttttc
     9541 ccaaattcgc cctcataatg ggggattata ttttgataga gagagcaaat ttatggcacc
     9601 aagaaaaaag aaagcgattg aattaccact actcccattg cgtgatgtgg tggtttttcc
     9661 ttatatggtt atgccgttat ttgtaggacg tgaaaaatca attcaagccc tacgctcggc
     9721 gatggattcc aataaacaac tgtttttagt cactcagcaa gatccaaata aagaagagcc
     9781 gaatgcagaa gatatgtatg gcgtaggtgt aattgccaac attattcaaa tgttaaacct
     9841 gccggacggc acagttaaag tgctggtaga aggacaaacc cgtgccaaaa tcgagcaaat
     9901 tcacgatgat gaaaacggct tctgggcggc aattcagcca atatattcgg aatatgatga
     9961 cgaaaacgaa gagttaaaag cgattgctaa aaccacttta accgaatttg aaaattacgt
    10021 taaaaataac aagaaaattc cggcggaaat tattgctaaa ctgcaaaaaa tcacgttgga
    10081 agaccgcttg gcggatacca ttgcctctaa cttaattgct ccggtgaaga aaaaacagga
    10141 attattggag caaccaaatc tgattgcccg ttttgaagcc ttgttaattg cgatggcaac
    10201 tgagatggat acgcttgaaa ccgaaactcg gattcgtaat cgtgttaaac agcaaatgga
    10261 aaaaaaccaa cgtgattact acctcaacga gcaaattaaa gcgattcaaa aagagttagg
    10321 taatggcgat gacgttgaac aaagcgagtt agataagctt aaagagaaaa ttgacgctgc
    10381 aaaattaccg accgaggtaa aagacaagct gaatacggag ttcaaaaagc tcaaagcaat
    10441 gccgcaaagc tcttccgaag ctaccgttgt gcgtggttat attgactgga ttttacaaat
    10501 gccgtggcat aaaaaatcag cggtcaaaaa agatttagcc caagctcaag agattttgga
    10561 taaagaccac tacggcttag aacgagtgaa agagcggatt gtagaatact tagcagtgca
    10621 aagtcgtcta aataaattaa aaggtccgat tttgtgctta gttggtccgc cgggggttgg
    10681 taaaacctca ttagggcaat ctattgccaa tgccacaggg cgtaaatatg tgcgtatggc
    10741 gttaggtggc gtgcgtgatg aagcggaaat ccgtgggcat cgccgtactt acatcggctc
    10801 aatgccgggt agcctaatga tgaaaatggc gaaagtcggc gtgcgtaatc cgctgttctt
    10861 gttagatgaa atcgacaaaa tggctcaaga tatgcgtggc gacccggctt ctgctctgct
    10921 tgaagtgtta gatcctgagc aaaataaagc ctttaacgac cattatttag aggtggatta
    10981 cgatttatcc gatgtaatgt ttgtggcaac ctcaaactca atgaacattc cgccagcatt
    11041 gctcgaccgt atggaagtga ttcgtctttc aggctatacc gaagatgaga aaatgcacat
    11101 tgctcgtggt catttactgg ctaaacagca agaaaataac ggactaaaag agggtgagct
    11161 gaccattgaa gacagtgcaa ttttaagtat tatccgctac tacacccgtg aagcaggcgt
    11221 gcgtagcctt gagcgtgaaa ttgcgaaaat ctgccgtaaa gccgtgaaag ctttagtatt
    11281 ggataagaaa ttaaaatcca ttacagtttc tgagaaaaat atcgaagaat acttaggcgt
    11341 aaaacgtttt gactacggca aaatggatag ccaaaaccgc attggcgaag tgaccggttt
    11401 agcgtggaca gaagttggcg gtgatttatt aaccattgaa accgcttctg ttgcaggtaa
    11461 aggcaaattc tcttacaccg gttcattagg cgatgtaatg aaagagtcta ttcaagcggc
    11521 aatggtggtt gtacgctctc gtgcagaaaa attaggcatt gctgaagact tccacgaaaa
    11581 acgggatatt cacgttcacg tgccggacgg tgcaacaccg aaagatggtc cgagtgcagg
    11641 gattgcaatg tgtactgcat taatttccag cctaacaggt aaccctgtgc gtaaagaagt
    11701 agcgatgacc ggtgaaatca gcttgcgtgg caaagtattg ccaatcggcg gcttaaaaga
    11761 gaaattactg gcagcccatc gtggtggtat tacgacggtg attattccga aagaaaatga
    11821 aaaagactta gaggaaattc cggaaaatgc aaaagcagcg ttggacattc acgcagtaga
    11881 aaccattgac gaggtactag ctctggcact agaaaacccg ccaatgggga ttgaaatctt
    11941 gtcaaaagcc gagatcaagg tgaaaaaatc gcgagctaaa gccacagtgc aataaagtgc
    12001 aataatctaa aacaacataa ggtgcgcaga tcacaatcac gcaccttatt ttttatctga
    12061 aagttttatc tacaagcggt cggttttgct ctattttttg caaaaattta gcaaaatcta
    12121 accgcttgtt atgctgaatt atcagacaat aattgccaaa atgcgttgat gatcggctct
    12181 tgcaagcgtt tttgctgaac gcaaattccc agctcaaacg gagtgatcgg cgagggtaaa
    12241 ttcacataag aaacttgatt attcattggg ctatgcttaa tcacaatatc cggtagcagt
    12301 gcgaccccaa agcctagtgc aaccatcggc acaatcgctt cgtgtccgga gacggtagca
    12361 taaataatcg ggtctttgat tttttgctct ttaaaccaac ggtcaatccg ttttcgtaca
    12421 ggtccgttca ccggcaaaat aaaggggata tttttccaat ctatcggcga ctgttgcaga
    12481 aattgagtgg cttggctcgc cacacgaggg gcaataatcg aaagttgaat atcatcaata
    12541 tagtgaaaca caatattatt cggcagaaat tccggcttac ccgcaaccga aatatctgca
    12601 gccaatgact gaacctgttc caatgccaaa gccggatcac cggtactcag tttaatatcc
    12661 actttcggat atttttggcg aaactttccc aaaatcggcg gtaaatggct gtaagaagcc
    12721 gttaccgagc agaacacttt caactctcct tctaaatcac cttgagcagg cgacaactgg
    12781 ttttgcaact gctgccactc tgcccaacct tgttgagcaa acgccaaaaa cctttcccct
    12841 gcttcagtta aacgcacttg tcgattatca cgcagaaaca acggctgccc gagctcctcc
    12901 tctatacgct gaatatggcg ggtaagtgtg gaagccgtca tataattctt ctccgcactg
    12961 cgaataaaac tacggctttg ggctatatct aggaaaagtt taaggctttg gaagtccata
    13021 ttattttagc cactccggtt ttattgcagc aaaatctaaa tcattcacgc tttttctaaa
    13081 acggagaata tgggtttcag cctgattatt cggatgccaa tgtataaaat tctgttcatc
    13141 gctactgtaa aaagcaatta tctttttact caagcctgag gcaatatgca cggttgcagt
    13201 atcaggtgaa attaataagt ccgcaaaacg aatcagttct attgaatcaa aaacgctttt
    13261 ggttttctca tagacaaata tatttttata cttcttagta aaggaacaca attttgccgt
    13321 tacttcagga aaagtaagta agactatcgg tttatcacta tgctgtttta agcaactcag
    13381 cagttcatta attttctgct cactaaaacg tcttgccgaa cctgcaccaa aaagatttaa
    13441 cgcaatataa tttctaattt ggttttcata taaataatgc tttatggatt ggttgcttgt
    13501 ggcatttagt ggcacatcat actgagtttc tatattttca aacccgatta aagctaacgc
    13561 atcttgataa atctctgaaa aatgctgttt tctatcctct acatttagat caaataatcg
    13621 atatttttgt ttctgataac ctacataatg tttggctgat atagtgcgta aaaataagag
    13681 atcacgattc cgtaaagtaa cagtcggatc gattacaaca tcatactgct cttttgcaag
    13741 ttgtttgcca ccacgaatat aatcaggaat actttttgtc ttaaccaaat agagcttatc
    13801 aatataagga ttattctcaa ataaatatcg gtttttctgt gagcaaacca cgcctatttg
    13861 cgtttgggga gactgttttt ttatttcacg gaagacaaag gaactaacga cataatcacc
    13921 aattttacca tcgtgacgaa gaaatagaat tttattggga atttccgata atatcagatc
    13981 agtattttta ctatctaaaa tggctttacc tatcaccaat cttgcttttc ttaacgttgc
    14041 tttaatattc
//
LOCUS       DANLRK010000056        14001 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.056, whole genome shotgun sequence.
ACCESSION   DANLRK010000056 DANLRK010000000
VERSION     DANLRK010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 14001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14001
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.056"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            35..808
                     /locus_tag="PXA33_001174"
     CDS             35..808
                     /locus_tag="PXA33_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL5120510.1"
                     /translation="MLKKLSIAALALTLSVSAFAKESLLERINNKGTITVGTEGTYAP
                     YTYHDASGKLTGYDVEVTRAVAEKLDVKVEFKETQWDAMLAGLKGERFDLVANQVALT
                     SPERRATFDKSESYSWDGAALLVREDETKIKTPADVKGVKTAQTLSSNYGEIARENGA
                     DIVPIDGMAQGLLLIQQKRADATFNSHLSLLDYLKKNPNSGLKIVWEKEEKVGAGLIA
                     NKGNDEALAKISKAIVELREDGTLKKLGEQFFGKDISVQ"
     gene            891..1607
                     /locus_tag="PXA33_001175"
     CDS             891..1607
                     /locus_tag="PXA33_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDL5120511.1"
                     /translation="MLNDLLLSIPFMTQARVDLVLSGFWPMVQAAFLVSIPLAIASFI
                     IGMLIAVAVALVRISPATGFFHRLLLWLVKGYISIIRGTPMLVQICIVFYGLPAIGIF
                     IDPIPAAIIGFSLNIGAYGSETIRAAISSVPKGQWEAGYTIGMTYMQTFRRIIAPQAF
                     RVAVPPLSNTFIGLFKDTSLASVVTVTEMFRVAQQVANMSYDFLPVYIEAGLIYWCFC
                     WVLFLIQARLEVRFNRFVAK"
     gene            1617..2369
                     /locus_tag="PXA33_001176"
     CDS             1617..2369
                     /locus_tag="PXA33_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDL5120512.1"
                     /translation="MLKIRNIHKTFGNNAILRGIDLDIQKGQVVVILGPSGSGKTTFL
                     RCLNALEMPEQGTLEFSGNAPLKIDFAVKQSKKEILALRRKSGMVFQNYNLFPHKTAL
                     ENVMEGPVFVQQQAVEIAQKNAKALLEKVGLADKAELYPFQLSGGQQQRVGIARALAI
                     QPELMLFDEPTSALDPELVQDVLNTMKELANEGWTMVVVTHEIKFALDVADVVVVMDG
                     GEIVEQGSPQQLFNHPQHERTKRFLNQIRADK"
     gene            complement(2585..3118)
                     /locus_tag="PXA33_001177"
     CDS             complement(2585..3118)
                     /locus_tag="PXA33_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HDL5120513.1"
                     /translation="MEFLAPVISLIVGFLSFVLVLRTWLQFCRVDPRLPISQTLLKIT
                     EPMVSPVGKLIPTVKNICFPALLIAAALVTLQFILFGVDLPRAVLIGLLSVVKTFGQI
                     LFFTTLIRALMSWVTQGNHPLDYTVAQITEPVLGFIRKLLPRTGMLDFSVMVLGFGLL
                     LLNSLFYQIFGGLWAAA"
     gene            complement(3224..3565)
                     /locus_tag="PXA33_001178"
     CDS             complement(3224..3565)
                     /locus_tag="PXA33_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HDL5120514.1"
                     /translation="MAIQRNARQRKKMHLGEYQELGFLIKFTFAEGTGIDQIDETVDR
                     FIDEVIRPNGLALEANGYLNWEGLVCLEKLGKCDESHRQLVKTWLETNGLKEVQVSEL
                     FDIWWDYPAQA"
     gene            complement(3589..4338)
                     /gene="trmB"
                     /locus_tag="PXA33_001179"
     CDS             complement(3589..4338)
                     /gene="trmB"
                     /locus_tag="PXA33_001179"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HDL5120515.1"
                     /translation="MSEKQTFADKKRKTVEQAEFTEDGRYLRKVRSFVLRTGRLSDYQ
                     RDMMNNNWANLGLDYQPMPFNFEQIFGNNNPVVLEIGFGMGKSLVEMAEQNPNRNYIG
                     IEVHTPGVGACIAYALEKGVKNLRVICHDATEILRDSIADGTLGGLQLYFPDPWQKAK
                     HHKRRIVQPEFISRVIRKLSPNGFIHFATDWENYAEHMLEVLRQFESELQNTSATNDF
                     IPRPNFRPLTKFEERGHRLGHGVWDLYFTKK"
     gene            complement(4348..5298)
                     /locus_tag="PXA33_001180"
     CDS             complement(4348..5298)
                     /locus_tag="PXA33_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HDL5120516.1"
                     /translation="MNSRILATGSYLPTKVRTNADLEKMVDTSDEWIVTRSGIRERRI
                     AEPDETVATMGAKAAQQALEMAGIEANDIDLIVVGTTTNSHAYPSAACQIQGLLQIED
                     AIAFDVAAACTGFVYALTVADQFVRNGQVKKALVIGSDINSRMLDETDRSTVVLFGDG
                     AGAVILEASEELGIISTHLHASPDLENNLTLRSQKRGEERSGFIEMQGNATFKIAVNQ
                     LSSVVEETLEANNLQKSDLDWLIPHQANLRIITATAKKLEMEMDQVVVTLDKYANTSG
                     ATVPVALDEAVRDGRIQRGQLLLLEAFGGGWTWGSVLVKF"
     gene            complement(5444..6463)
                     /gene="hemB"
                     /locus_tag="PXA33_001181"
     CDS             complement(5444..6463)
                     /gene="hemB"
                     /locus_tag="PXA33_001181"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HDL5120517.1"
                     /translation="MQVIQSQYPLRRMRRLRKHDFSRRLVAENQLTVNDLIYPVFVIE
                     GENQRVQVPSMPGVERLTIDQLLVEAAELVKYGVPAVALFPVVGDVKKSLMAEEAYNP
                     QGLAQRAVRALKAQFPELGVITDVALDPFTTHGQDGIIDETGYVLNDVTTEVLVKQAL
                     SHAEAGADIVAPSDMMDGRIGKIREALEQNGFINTQIMAYSAKYASNYYGPFRDAVGS
                     AGNLKGGNKYTYQVDPANGNEGLHEVAMDIQEGADMVMVKPGMPYLDMVWRVKENFGV
                     PTFAYQVSGEYAMHMAAIQNGWLKERECIMEGLLCFKRAGADGILTYFAKRVAKWLYE
                     EAQAK"
     gene            complement(6544..7299)
                     /gene="tatC"
                     /locus_tag="PXA33_001182"
     CDS             complement(6544..7299)
                     /gene="tatC"
                     /locus_tag="PXA33_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HDL5120518.1"
                     /translation="MSQVDESQPLITHLIELRNRLLRSFICVLLVFCGLVYWANDIYT
                     LLATPLTDNLPDGATMIATNVATPFFTPIKLTIVVSVFLSVPFILYQIWAFVAPALYK
                     QEKRLVYPLLVSSTLLFYCGVAFAYYVVFPLVFGFLTSTAPEGVTMATDISSYLDFVL
                     TIFMAFGICFEVPVAIILLCWAGVTTPEDLKAKRPYIIVAAFVIGMLLTPPDIFSQTL
                     LAIPMCLLFEVGVLFARFYRPKDDDEPSEQVAQ"
     gene            complement(7286..7930)
                     /gene="tatB"
                     /locus_tag="PXA33_001183"
     CDS             complement(7286..7930)
                     /gene="tatB"
                     /locus_tag="PXA33_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HDL5120519.1"
                     /translation="MFDIGFSELVLIMIVGLVVLGPKRMPIAIRTVMGWVSTIRGLAA
                     NVQNELAQELKLQELKESIKKAEELNLSSLSPELNKTVEDLKMSATQLQDSLNSAKNE
                     LEKTAAESSTIPKLTDEQVAEIQQKIEQESQVLEAQELQKNAQNKPLEVDSLSEQLNV
                     TRTEKPLSADELAEQAEMDESLLTLESYYPPDDDLATPQSSESAQKSEANNVAS"
     gene            complement(7934..8155)
                     /gene="tatA"
                     /locus_tag="PXA33_001184"
     CDS             complement(7934..8155)
                     /gene="tatA"
                     /locus_tag="PXA33_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011199679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HDL5120520.1"
                     /translation="MGGISIWQLLIVVAIIVLLFGTKKLRTLGSDLGESVKGFKKAMA
                     DEKKDAEFEKVEAKDAISAEQKAKEKEQA"
     gene            complement(8266..9903)
                     /gene="ubiB"
                     /locus_tag="PXA33_001185"
     CDS             complement(8266..9903)
                     /gene="ubiB"
                     /locus_tag="PXA33_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HDL5120521.1"
                     /translation="MNIKNLNRFYHIIRTFLRYGIDEAIPNLPITRKIKLGRKALFWV
                     KNQHSDKPYGVRLRLALQELGPVWIKLGQMLATRRDLFAPELADQLALLQDNVEPFDG
                     KIARQIIEQALGGKLETWFENFDETALASASIAQVHTAKFNQNQPLAGKDVVLKVIRP
                     GIEPVIKADLELMYEIAHFIPKLSNDGKRLRAVEVVREYEKTILDELDLRKEMHNAIR
                     LRANFENSEMLYVPEMYQDFCHKNLIAMERIYGIPVSNIAELEANSTDMKLLAERGVQ
                     VFFTQVFRDSFFHADMHAGNIFVNREHPENPQYIGIDCGIVGTLNHNDKRYLAESFVA
                     FFNRDYRRVALMHVESGWTPPDTDIDKFEQAFREVCEPIFAKPLSEISFGHVLLNLFN
                     VAREFNMEVQPQLVLLQKTLLYIEGLGRQVYPQLDLWQTAKPFLQNWLNEQVGVKAMV
                     RDIKQRLPQYREHFAEFPEALFKALQEQKQINFRLAEINESLKAQNSNANSRFLVLSI
                     SAIVICGAFWKFESLPIWLSVPLLTLFPLVLILSQRK"
     gene            complement(9977..10954)
                     /locus_tag="PXA33_001186"
     CDS             complement(9977..10954)
                     /locus_tag="PXA33_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005825116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HDL5120522.1"
                     /translation="MKQIIAQARENAVRAVDFQRVLMYWHIGKRIFEQEQQGQERADY
                     GTYLIKMLAKELEPEFGSGFCSRQLERYRQFYRAFPIATALRSQLNWTQYRRLIQISD
                     SDKREFYLAESCKNNWSSRQLERQINSSLYERLLLSNDKESVLAVARNEQQPVDPKQL
                     IKDPMVLEFLGLKRESAYYEKDLEQAIITHLQDFLLELGNGFSFIARQKRLHLDGDDF
                     FVDLVCYNRLLQCFVIFEIKTHKLTHQDLGQLQMYVNYFDRVEKLPHENATIGVLLCA
                     DKNDAVVKFSLPENQNKIFASQYQLYLPSEQTLIAEVQKEIENFEQKYP"
     gene            complement(11115..11762)
                     /locus_tag="PXA33_001187"
     CDS             complement(11115..11762)
                     /locus_tag="PXA33_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HDL5120523.1"
                     /translation="MLNNVKQQLMLPQFTFGLIETAFNALLKRSPHIEPNLRKLSGKV
                     LKIALTSPKIDFFVLFSESRTDWLGNYEGEADCTVQLAFDTLPKLADKQKLTELINNK
                     SLVLTGDIQVLQHFTALLEQLEKDPAELLSPFVGDVVAQTSTDLAKKLLGKVKAQFEQ
                     NNQHLVENLIQERPVLVHRLQAVHFYDQVEELAQQALKLEQKFTHYVRSSSHQQS"
     gene            complement(11953..12705)
                     /gene="ubiE"
                     /locus_tag="PXA33_001188"
     CDS             complement(11953..12705)
                     /gene="ubiE"
                     /locus_tag="PXA33_001188"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HDL5120524.1"
                     /translation="MQQSAETTHFGFKTVAKEEKQQLVANVFHSVAGKYDLMNDLLSF
                     GIHRVWKRFTIDCSGVRKGQKVLDLAGGTGDFTAKFSRIVGESGEVILADINSSMLEV
                     GREKLRNLGVVGNVSYVQANAECLPFADNTFDCIVISFGLRNVTDKDKALRSMFRVLK
                     PGGRLLVLEFSKPIIDPISQLYNFYSFNILPKVGEVVVNDAESYRYLAESIRMHPKQD
                     ELKAMMEQTGFESVNYYNLSAGIVALHRGYKF"
     gene            13050..>14001
                     /gene="corA"
                     /locus_tag="PXA33_001189"
     CDS             13050..>14001
                     /gene="corA"
                     /locus_tag="PXA33_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011199502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HDL5120525.1"
                     /translation="MIRAFGLDNACLISVDETNPTLLNDAIWIDLIDPSEAERTVLEN
                     RLDQTLAEERELEDLEASARFFEDEDGLHLHSFFYCLDDDDYADIATVAFTIRDGRLF
                     TLRDRDLPAFRLYRMRSRREKLIDSNAYELLLDLFETKIEQLADVIEEVYADLEKLSR
                     VILNGQQENENFNDALSRLTEFEDASSKVRLCLMDTQRALSFLLRKTRLPNNQLEQAR
                     DIMRDIESLQPHNESLFQKVNFLMQAAMGYINIEQNRIMKFFSVVSVMFLPATLVAST
                     YGMNFEFMPELHFQYGYPMAIGLMIVAASAPYLYFKRKGWL"
BASE COUNT         3960 a         3145 c         2758 g         4138 t
ORIGIN      
        1 aaaaatttat tttaccaatt aaaaggaaat gattatgttg aaaaaattat caattgctgc
       61 attagctctt accctttctg tttctgcatt tgctaaagag agcctacttg agcgtataaa
      121 caacaaaggg actattactg tgggtacgga aggaacttat gccccttaca cctatcacga
      181 tgcgagcggt aaattaaccg gttacgatgt agaagtaacc cgtgcggtgg ctgaaaaatt
      241 agacgtaaaa gtggaattta aagagacaca atgggacgct atgttagccg gtttaaaagg
      301 tgagcgtttt gacttagtgg caaaccaagt ggctttaacc agcccggaac gtcgagcgac
      361 atttgataaa tccgaatctt acagctggga cggtgccgcc ttattagtgc gtgaagatga
      421 aactaaaatc aaaacccctg ctgatgtaaa aggcgtgaaa actgcccaaa ccttgagctc
      481 aaactatggc gagattgccc gtgaaaacgg tgcagacatc gtgccaattg acggtatggc
      541 tcaaggctta ctgttaattc aacaaaaacg cgccgatgca actttcaata gccacttatc
      601 gctattagat tacttgaaaa agaacccgaa ttccggctta aaaattgtgt gggaaaaaga
      661 agaaaaagtg ggtgcaggct taattgcgaa taaaggcaat gacgaagcat tagcgaaaat
      721 cagcaaagca atcgtagaat tacgtgaaga cggcacgtta aaaaaattgg gcgagcagtt
      781 cttcggtaaa gatattagtg tacaataatc catattggat taggcttggg gcgaattcgc
      841 cccttttatt tttaaatctc cctcaaaacg ggagatttta ggtttttcag atgttaaacg
      901 atctacttct ctcaatcccc tttatgaccc aagctcgggt cgatttagtg ctaagcggtt
      961 tttggccgat ggtacaagcg gcatttttag tctctattcc gctggcaatc gcctcattta
     1021 ttatcggtat gttaattgcc gttgcagttg cattagtgcg aattagccct gcaacaggct
     1081 tttttcaccg cttgctactg tggctggtaa aaggttatat ctcgattatt cgcggcacgc
     1141 cgatgttggt tcagatctgt attgtgtttt atggtttgcc tgcaatcggc atttttatcg
     1201 atccgattcc ggcggcaatt atcgggtttt cactcaatat cggggcttat ggctcggaaa
     1261 cgatccgtgc tgcgatctcc tccgtgccaa aaggacaatg ggaagccggt tatactatcg
     1321 gtatgactta tatgcaaact tttcgccgta tcattgcccc gcaagcgttt cgagttgctg
     1381 ttccgccact tagcaacacc tttatcggtc tgtttaaaga tacctctctt gcctccgttg
     1441 ttaccgtaac cgaaatgttc cgggttgctc aacaggtagc aaatatgtct tatgacttcc
     1501 tacctgttta tattgaagcc ggtttgattt attggtgctt ctgctgggtg ctatttttaa
     1561 ttcaggcgag attggaagtc cgctttaacc gatttgtggc gaaataagga ggaattgtgc
     1621 ttaaaattcg caatattcat aaaacctttg gcaacaacgc tattctacgc ggcattgatt
     1681 tagatattca aaaagggcaa gtggtagtaa ttttaggccc ttccggttca ggcaaaacca
     1741 cctttcttcg ttgcttaaat gcactggaaa tgcccgagca aggcacattg gaatttagtg
     1801 gtaatgctcc gctgaaaatt gattttgccg taaaacagag caaaaaagag attttagcgc
     1861 tacgccgcaa atccggtatg gtgttccaaa actacaacct gttcccgcat aaaaccgcat
     1921 tggaaaatgt gatggaagga ccggtatttg tgcaacaaca agcggtcgaa attgctcaaa
     1981 aaaatgcaaa agccctgtta gaaaaagtcg gactggcgga taaagcagaa ctctacccgt
     2041 ttcaactgtc aggcgggcaa cagcaacggg ttggcattgc acgagcatta gcaattcagc
     2101 cggagctgat gttatttgat gaaccaactt cggcacttga tcctgagtta gtgcaagacg
     2161 tgctaaatac gatgaaagaa ctggcaaatg aaggctggac aatggtcgtg gttactcacg
     2221 aaatcaaatt tgcccttgat gttgccgatg tagtggtggt aatggacggc ggtgaaatcg
     2281 ttgaacaagg ctcgccacag caactgttta accacccaca acacgaacga actaaacgtt
     2341 ttttaaatca aatcagagcg gataaataag aaacgatagc gctagcgtcc acgctagtgc
     2401 tatctaataa atggtacaag cggttaatta tgtataaatt gttatactgt tttggcacga
     2461 gcgtggacgc tcgcgccatc gtacagtaaa atcagctaac aaggttcccc ataaaggata
     2521 aataagacaa ggctccttaa tcttttagat taaggagctt atttttagat tttagaaatc
     2581 cgatttaagc cgcagcccat agtccaccaa aaatttggta gaacaagcta tttagtagca
     2641 ataagccgaa acctagcacc attaccgaga aatctaacat tcccgttctt ggcaggagct
     2701 tgcggataaa acctaacacc ggttcggtaa tttgtgctac cgtgtagtct aatgggtggt
     2761 tgccttgagt gacccagctc attaatgcac gaatcaaggt ggtgaaaaat agaatttgtc
     2821 cgaaggtttt gaccacactt aataagccga ttaatactgc tctgggtaaa tctacgccaa
     2881 acagaataaa ctgtaacgtt accaaagcgg cggcaattaa tagtgccgga aagcaaatat
     2941 ttttaacggt tggaatcagc ttgccaaccg gtgaaaccat cggttcggta attttaagta
     3001 aggtttgtga aatcggtaaa cgaggatcaa cccgacaaaa ttgcagccaa gttcttagca
     3061 ccagcacaaa acttaaaaag ccaacaataa gcgaaataac aggagctaaa aattccatct
     3121 ctttttctct ttctatacaa aacaagcggt cgttttttag aaaaaatttg caaattttca
     3181 gtaaaatctg accgcttgta tcttattaaa ttaactaggc aaattaagct tgtgctgggt
     3241 agtcccacca aatatcaaat aattcgctca cttgaacctc ttttaagccg ttagtttcta
     3301 accaagtttt cactaattgg cggtgagatt catcacattt gcctaatttt tctaagcaga
     3361 ctaaaccttc ccagtttaag tagccgttcg cttcaagggc taaaccgttt ggtcgaatca
     3421 cttcatcgat aaaacgatct acggtttcat caatttggtc aatgcctgtg ccttcagcaa
     3481 aggtgaattt aattaagaaa cctaattctt ggtattcacc taagtgcatt tttttacgtt
     3541 ggcgtgcgtt acgttgaata gccatttttt ctctcctcta tgaataaatc atttctttgt
     3601 aaaatataaa tcccacaccc cgtgtcctaa acgatgccct cgctcttcaa atttggttag
     3661 cgggcggaaa ttcggacgtg gaataaaatc attagttgct gatgtatttt gcaactcgct
     3721 ctcaaattgg cgtaatactt caagcatatg ttctgcgtaa ttttcccagt cggtggcaaa
     3781 atgaataaag ccgttagggc ttaattttct gatcacacgg ctgataaatt caggctgcac
     3841 aatacggcgt ttgtggtgtt ttgctttttg ccaagggtct gggaagtaaa gctgtaagcc
     3901 acctaatgta ccgtcagcaa tgctgtctcg caaaatttcg gtggcatcgt ggcaaattac
     3961 tcttaaattc ttcacgcctt tttctaacgc ataagcgata caagccccca ccccgggcgt
     4021 gtgcacctca atgccgatat agttgcgatt cgggttttgc tctgccattt ccaccagcga
     4081 ttttcccatt ccgaagccga tttctaacac taccgggttg ttattgccga aaatctgctc
     4141 aaaattaaag ggcattggct ggtaatctaa gcctaaattt gcccagttgt tgttcatcat
     4201 atcacgttga taatcgctca aacgaccggt acgcaataca aaactccgca cttttcgtaa
     4261 ataacgccca tcttcggtaa actcggcttg ctcaacggtt ttacgttttt tatctgcaaa
     4321 agtctgtttt tctgacattt tcttgcctta aaacttcact aataccgaac cccaagtcca
     4381 gccaccgccg aatgcttcaa ggagtagtaa ttgcccacgt tgaattcgcc cgtcacgcac
     4441 cgcttcatct aatgccaccg gcactgttgc tccactggta ttggcatatt tatcaagggt
     4501 aaccaccact tgatccattt ccatctctaa ttttttcgcc gttgcggtga taatgcgtaa
     4561 attcgcttgg tgcggaatca gccaatctaa atcggatttt tgtagattat tcgcttctaa
     4621 ggtttcttct accacgcttg aaagttggtt tactgcaatt ttaaaggtgg cattgccctg
     4681 catttcaata aagccggaac gctcttcgcc acgcttttgg cttcttaagg ttaagttatt
     4741 ttctaaatcc ggtgaggcgt gcaagtgggt tgaaataata cccagctctt cactcgcctc
     4801 taaaatcacc gccccagctc cgtcaccaaa taatactact gtgctgcggt cggtttcgtc
     4861 taacattctg gaattgatgt ctgaaccaat aaccaatgcc tttttcactt gcccgttacg
     4921 cacgaattga tcggcaacag ttaaagcata cacaaatcct gtacaagccg ctgccacatc
     4981 aaaagcaatc gcgtcttcaa tctgaagcaa gccttgaatt tggcaagctg cacttgggta
     5041 agcgtgggag ttggtagttg ttcccaccac aattaaatca atgtcatttg cctcaattcc
     5101 tgccatttcc agtgcttgtt gagcggcttt tgcccccatt gttgccactg tttcgtcagg
     5161 ctcggcaata cggcgttcac gaatgccgga acgggttacg atccattcat cagaggtatc
     5221 gaccattttt tctaaatcag cattggtacg cacctttgtc ggtaaatagc ttcccgttgc
     5281 taaaattcta ctattcatat ttttattatt ctcaatcaat taaatgataa tccgttatta
     5341 tagcttgaaa accaacaaat atataggctt tacaagcggt catttttagc aaaaaagttg
     5401 cattcacgaa cgattttgca ctagaaccac cgcttgctag tatttatttc gcttgagcct
     5461 cttcgtatag ccacttcgct actcgcttgg cgaaataagt tagaataccg tctgctcctg
     5521 cccgtttaaa gcagagtaat ccctccataa tgcactcacg ctctttcagc cagccgtttt
     5581 ggattgccgc catatgcatc gcgtattcgc ccgaaacttg gtaggcgaag gttggcacgc
     5641 caaaattctc tttcacacgc cacaccatat ccaaataagg catacccggt ttcaccatca
     5701 ccatatctgc cccttcttga atatccatcg ccacttcgtg caagccttcg ttgccgttag
     5761 ccggatcgac ttggtaagtg tatttattac cgccttttaa gttgccggca gaacctactg
     5821 catcacggaa cggtccgtaa taattagacg catatttagc cgaatacgcc ataatttgcg
     5881 tattgataaa accgttttgc tcaagggctt cacgaatttt gccgattcga ccgtccatca
     5941 tatcgctcgg ggcaacaata tccgcccccg cttctgcgtg cgataacgcc tgttttacca
     6001 atacttcagt agtcacatca ttcagtacat agcctgtttc atcaataatg ccgtcttgtc
     6061 cgtgcgtggt aaacgggtct aacgccacat cagtaatcac gcctaattcc ggaaattgag
     6121 cttttaaagc tcgcacggca cgctgtgcca acccttgcgg gttataggcc tcttctgcca
     6181 tcagtgattt tttcacatca cccaccacag ggaaaagagc caccgcaggc acgccatatt
     6241 taaccagttc tgcggcttca actaaaagct ggtcgatagt taaacgctct acacccggca
     6301 tagaaggcac ttgtactcgc tgattttccc cttcgatgac aaaaacaggg taaatcaaat
     6361 cattaacggt aagttgattt tctgccacta agcggcggct gaaatcgtgc ttgcgtaaac
     6421 gacgcatacg gcgtaaagga tattgggatt gaattacttg catttttaat ttcctaattc
     6481 ttaaaatacc tattcttgaa ataagaaagg catagcaaac aggtgctatg ccttttcaaa
     6541 atattactgt gcaacctgct ctgatggttc atcatcatct ttggggcgat aaaaccgagc
     6601 aaatagcaca ccaacttcaa acaataaaca cattggaatt gccaataatg tttgcgagaa
     6661 aatatctggt ggggtgagca acataccaat tacaaatgct gccacaataa tataagggcg
     6721 ttttgccttt aaatcttcgg gtgtggttac gcctgcccaa cataacaaga taatcgcaac
     6781 cggcacttca aagcagatcc cgaatgccat aaaaatggtt aaaacgaaat caagatagct
     6841 actaatatcc gttgccattg ttaccccttc cggagcggta gaggttaaaa agccaaatac
     6901 aagcggaaat acaacataat aggcaaaagc gacaccacaa taaaacagca atgtgctaga
     6961 taccaataat gggtaaacca aacgcttttc ctgcttgtat aaagcagggg cgacaaacgc
     7021 ccaaatttgg tacaaaataa aaggaactga taaaaacacc gaaaccacaa tggttaattt
     7081 aattggggta aaaaacggag tggctacatt ggttgcaatc attgttgcac cgtctggtaa
     7141 attgtccgtt aaaggtgttg ccaataaggt gtaaatatca ttggcccagt aaaccaatcc
     7201 gcaaaacacc aagagcacac agataaaact acgcagtaat cggtttctca gctcaattaa
     7261 atgtgtaatc agaggttgag actcatcaac ttgcgacatt atttgcctcc gatttctgcg
     7321 ctgattctga tgattgaggc gtagctaaat catcatctgg cggatagtaa gattccaacg
     7381 ttaaaagcga ttcgtccatt tctgcttgtt cagccagctc atcagccgaa agtggctttt
     7441 ctgtccgcgt aacattaagt tgctcagata aactatccac ttcaagcggt ttattttgcg
     7501 catttttttg caattcttgc gcctctaaca cctggctttc ttgctcgatt ttttgctgaa
     7561 tttctgcgac ttgctcatcg gttaatttag gaattgtgct gctttccgca gccgtttttt
     7621 ccaactcatt ttttgcagaa ttaagcgaat cttgcaactg ggtggctgac attttcagat
     7681 cttccaccgt tttatttaat tctggcgaaa gggagcttaa attcaactct tctgcctttt
     7741 taatgctctc ttttaattct tgcagtttca gctcttgagc cagttcattt tgtacgtttg
     7801 cggctaaacc tcgaattgta ctcacccagc ccattacggt acgaatcgca atcggcatac
     7861 gtttcgggcc tagcaccacc aagcccacaa tcataatcag tactaattca gaaaaaccta
     7921 tatcaaacac ggattaagcc tgctctttct cttttgcttt ctgctctgct gaaattgcat
     7981 cttttgcttc cactttttca aattcagcat cttttttctc atctgccatt gcttttttga
     8041 agccttttac agattcgccc aaatccgaac ctaaagtacg taatttcttc gtaccgaaaa
     8101 gtaatacgat gattgcaacc acaatcagaa gctgccaaat actaataccg cccataaaaa
     8161 ctcctttcat ttaataggga atagataaaa taaaacgctg tttattatat gttactttga
     8221 catataaaaa agtaattttg caaaaaattt gtcaaaaaca accgcttatt tgcgttgact
     8281 caaaatcaat accagtggaa ataaagttaa taacggcaca cttagccata tcggcaaaga
     8341 ttcaaatttc caaaatgcgc cacagatcac aatcgctgaa attgacagca caagaaaacg
     8401 gctatttgcg tttgaattct gtgcttttaa actttcgtta atttccgcta aacggaaatt
     8461 aatttgcttt tgctcttgta aggctttaaa caatgcctcc ggaaactccg caaaatgctc
     8521 ccgatattgc ggtaaacgtt gcttaatgtc tcgaaccatc gctttcacac ctacctgctc
     8581 attcagccag ttttgtaaaa acggttttgc ggtttgccat aaatccaact gtggataaac
     8641 ctgcctgccc aaaccttcaa tataaagaag tgttttttgc aacaacacca actgcggctg
     8701 cacttccata ttaaattctc ttgccacatt aaacaggttg agcaaaacgt gtccgaatga
     8761 aatttccgat aacggcttag caaaaatcgg ctcacatacc tcacgaaagg cttgctcaaa
     8821 tttatcaata tcggtatccg gcggcgtcca gcccgattcc acgtgcatta aggcaacacg
     8881 gcggtaatct cggttgaaaa atgcgacaaa actttcggct aaatagcgtt tgtcattgtg
     8941 gttcaacgtg ccgacaatcc cgcagtcaat gccgatatat tgcggatttt ccgggtgttc
     9001 tctgttcaca aaaatattgc cggcgtgcat atcggcgtgg aaaaaactgt cacggaatac
     9061 ttgggtaaaa aatacctgca caccacgctc tgccagcaat ttcatatcgg tgctgttcgc
     9121 ttccaactcg gcaatgttag acactggaat gccataaatc cgctccatcg caatcaagtt
     9181 tttatggcaa aaatcttgat acatttcagg cacatacagc atttcgctat tttcaaaatt
     9241 cgcccgaagc cgaatggcat tgtgcatttc tttgcgtaaa tccagctcgt ctaaaatagt
     9301 tttttcgtac tctcgcacca cttcaaccgc ccgtagccgt ttgccgtcat tcgataattt
     9361 cgggataaag tgagcaattt catacatcaa ctctaaatcc gccttaatca ccggctcaat
     9421 gcccgggcga atcactttta acaccacatc tttgcctgca agcggttgat tttggttaaa
     9481 ttttgccgtg tgaacctgtg caatcgaagc agaagctaag gcagtttcat caaagttttc
     9541 aaaccaagtt tcaagctttc cgcccaaagc ctgctcgata atttgacgag cgattttgcc
     9601 gtcaaacggt tcaacattat cttgtagcaa ggcgagctgg tcggcaagct ccggtgcaaa
     9661 cagatcacgt ctggtagcta acatttgccc gagcttgatc caaaccggtc caagctcttg
     9721 caaagccaaa cgtaaacgta caccgtaagg tttgtctgaa tgttgattct tcacccaaaa
     9781 caaagcctta cgccctagct ttattttgcg ggtaattggc agattcggaa tggcttcatc
     9841 aataccatag cgaagaaaag tgcggataat atggtaaaag cggttgagat ttttgatgtt
     9901 cattgaaata tatatttaat ggtaattagg aagattttag caaaattctg taaaaattta
     9961 accgcttgtt gggctactat gggtatttct gctcaaaatt ctcaatttct ttctgtactt
    10021 ccgcaataag tgtttgttca gaggggaggt aaagttggta ttggctggca aaaattttat
    10081 tctgattttc cggtagcgaa aattttacca ccgcatcatt tttatcggca caaagcaaga
    10141 caccaatagt ggcattttcg tgcggcagtt tttcaactcg gtcaaaataa ttgacgtaca
    10201 tctgtaattg ccccaaatct tgatgggtga gcttgtgggt tttaatttca aaaatcacaa
    10261 aacattgaag taaacggttg taacaaacca aatcgacaaa aaaatcatca ccgtctaggt
    10321 gcaaacgctt ttggcgtgcg ataaacgaga aaccgttacc caattctagt agaaaatcct
    10381 gcaaatgcgt aatgatggct tgctctaaat ccttttcgta ataagcactt tcccgtttta
    10441 gtccgagaaa ttccaatacc atcgggtctt taatgagctg tttcggatca accggctgct
    10501 gctcatttct ggcaacagca agcacacttt ccttatcatt actgagcaat aatcgctcgt
    10561 atagactact attaatttgc cgttccagct ggcgtgaact ccaattattc ttacaacttt
    10621 cagccaaata aaattccctt ttgtcagaat cggagatttg aattagacga cgatattgtg
    10681 tccaattcaa ttgcgaccgc agtgcggtcg caattggaaa cgcacgataa aactggcgat
    10741 aacgctctaa ttgacggcta caaaaaccac ttccaaattc tggctcaagc tcttttgcga
    10801 gcattttaat caggtatgtt ccataatcag cacgctcttg cccttgttgc tcctgttcaa
    10861 aaatccgttt tccgatgtgc caatacatca gcacacgttg gaaatctacc gcacgaaccg
    10921 cattttcacg agcttgagca ataatttgtt taatatctta gatcatcgtt gtttctatta
    10981 acataaaagc ctccgttttg gattaagttt atgcttaatc tatccggagg caatttcttt
    11041 cactcaactt ttcgagtcat atcacaattt tattttgtta aatttaattt tgcacaaacg
    11101 cttgcgtttg aacatcagct ttgctgatgg ctcgaagagc gaacgtagtg agtaaatttt
    11161 tgctctaatt tgagcgcttg ttgggcaagt tcttcgactt ggtcatagaa atgcaccgcc
    11221 tgtaaacgat gcaccaacac cggtctttct tggattaaat tctcaaccaa atgttgatta
    11281 ttttgctcaa attgagcttt tactttacct agcaattttt tagcaagatc ggtgctagtt
    11341 tgagccacta catcgccaac aaaaggggaa agcagttcgg cagggtcttt ttcgagctgt
    11401 tcaagtaatg cagtgaaatg ttgcaacact tgaatatcgc ccgtcagcac caacgattta
    11461 ttattaatca gctcggttaa tttttgctta tccgctagtt tcggcagggt gtcaaatgcc
    11521 agttgcaccg tacaatctgc ttccccctcg tagttgccaa gccaatcggt tcggctttcg
    11581 ctaaacaaca caaaaaagtc gattttaggc gaagttaggg cgatttttag cacttttccg
    11641 gacaatttac gcaaattcgg ctcaatatga ggcgagcgtt taagtaacgc attaaaagcg
    11701 gtttcaatca gaccaaaggt aaattgcggg agcattaatt gttgttttac attatttaac
    11761 attcgtacct acttaccctc ttcacttttg ggagaaggat agattgttct tcattaagaa
    11821 gcaaaatcag ggagaggggt atttgcaaaa tattgaataa atatgaccgc ttgccccctc
    11881 tctctgctta tttcgctaaa cgctcataag cggtctctct cccacaaagg gagagagtca
    11941 tttaagagaa aactaaaatt tataacctct gtgtaatgcc acaatgccgg cacttaagtt
    12001 gtagtaattc acgctttcaa agccagtttg ttccatcatt gcttttagct cgtcttgttt
    12061 cgggtgcatt cggatcgatt cagctaaata gcggtaactt tcggcgtcat ttaccaccac
    12121 ttcgcccact ttcggcaaaa tattgaatga gtagaaatta taaagctggc tgattgggtc
    12181 gataatcggt tttgagaatt ccaacacaag taagcgaccg cccggtttta gcacgcggaa
    12241 cattgaacgt agggctttgt ctttgtcggt aacattacgc aagccaaaac taatcacaat
    12301 acaatcaaaa gtgttatcag caaaaggcaa gcattcggca ttcgcttgaa cgtagctcac
    12361 atttccaacc acgcctaaat tgcgtaattt ttcccgcccg acttccaaca ttgagctgtt
    12421 aatatcggct aaaatcacct caccactttc accaacaatg cgagagaatt ttgcggtaaa
    12481 atcacccgtg ccgccggcaa gatccagcac tttttgccct ttacgcacgc cactgcaatc
    12541 aatggtaaag cgtttccaca ctcgatgaat gccgaaagaa agcaaatcgt tcattaggtc
    12601 atatttgcct gccaccgagt gaaacacatt tgcgaccagt tgctgttttt cttctttcgc
    12661 tacggtttta aacccaaagt gggtggtttc tgccgattgt tgcacttctt gttgcacttc
    12721 atctttaggt gctaattgtt ctacatcgct cattttcttg tctctagttg caaaatttag
    12781 gcgtaaccat agcaaaaaag cggtcgtttt tctttaactt tttgcaaatt ttcttaagta
    12841 aataaccgct ttcttgacta gaaaagccaa attctgtaac attcgcacac tattttttct
    12901 tgaggattac acattttgga cagtcatagt cgatacttac ccgtttaacc tatcttgtct
    12961 aacctcaatg ttaccgacaa ggtcggtaat gctcgcctag tatctttgtt cctttttgtt
    13021 ccaagcctcc taactatttg gagctaaaca tgatccgtgc ttttggacta gataacgcat
    13081 gtttaatcag cgttgatgaa actaacccaa ctttattaaa tgatgcgatt tggattgatc
    13141 tcatcgatcc atcagaagct gaacgaaccg tattagaaaa ccgcttagac caaacactcg
    13201 ctgaagaacg tgaattggaa gacttagagg cgtctgcccg tttctttgaa gatgaagacg
    13261 gtttgcacct gcactcgttc ttttactgct tagatgatga cgattacgcc gatattgcca
    13321 ctgtcgcctt cactattcgt gacgggcgtt tgtttacctt gcgtgatcgg gatttacccg
    13381 catttcgctt gtatcgaatg cgttcccgcc gtgagaaatt gattgacagc aacgcctatg
    13441 aactcttact cgatttattt gagaccaaaa tcgagcagct tgcagacgta attgaagaag
    13501 tctatgctga cttagaaaaa ttaagtcggg ttattctaaa cggtcagcaa gaaaatgaga
    13561 atttcaatga tgccctttct cgcttaactg aatttgaaga tgcaagttca aaagtgcgtt
    13621 tatgtttgat ggatacgcaa cgggcgttaa gtttcttact gcgtaaaacc cgtttgccga
    13681 ataatcagct agaacaagcc cgtgacatta tgcgagatat tgaatctctg caaccgcaca
    13741 atgaatcctt gttccaaaaa gtgaattttt taatgcaagc ggcaatgggt tatatcaata
    13801 ttgagcagaa ccgaattatg aaattcttct cggtggtgtc agtgatgttc ctacctgcta
    13861 cgctggttgc ttctacttac ggtatgaact ttgaatttat gccggaactt catttccaat
    13921 atggctaccc tatggcgatt ggtttaatga ttgttgccgc ctccgcccct tatctttatt
    13981 ttaaacgtaa aggttggtta t
//
LOCUS       DANLRK010000057        13934 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.057, whole genome shotgun sequence.
ACCESSION   DANLRK010000057 DANLRK010000000
VERSION     DANLRK010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 13934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13934
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.057"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(<1..601)
                     /locus_tag="PXA33_001190"
     CDS             complement(<1..601)
                     /locus_tag="PXA33_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HDL5120526.1"
                     /translation="MTELTPKQEAFCFAYIETGNASEAYRQAYETEDMKSETVHRKAK
                     ELMDNGKITARIEELKAEHAERHKLTVDDLLKELEEARILAREKENPNAMTQATMGKA
                     KLLGLDKQVTDPCFDGETGKGDMILSVNCSSIKDGKALDETCILKAGTHEFITRDSFI
                     FYRHIRIDSLDEIKANIEAGYFIQKALINDEAYQQILKGV"
     gene            complement(598..1023)
                     /locus_tag="PXA33_001191"
     CDS             complement(598..1023)
                     /locus_tag="PXA33_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5120527.1"
                     /translation="MKHELNLAGITALMNEPIKATLSFLDFPHIRIERLHHDNGRPFF
                     IGVLSLDLEDHINIEMAGATGFLEALAIIAYHQQLTATPLLVNDDLLSEINKLPHPQR
                     TNPFIDRHGNEITREQMSKEYREKIDYAFAEKRMAEGKE"
     gene            complement(1030..1182)
                     /locus_tag="PXA33_001192"
     CDS             complement(1030..1182)
                     /locus_tag="PXA33_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5120528.1"
                     /translation="MPELLIVLGNRIAKLEQQHNAMPSETDSITLGILRKQLSQLLKV
                     AERIKE"
     gene            complement(1343..2029)
                     /locus_tag="PXA33_001193"
     CDS             complement(1343..2029)
                     /locus_tag="PXA33_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5120529.1"
                     /translation="MADHSQFISRLENRIEPKISFQELIQELSNYTNKPFSHIADKLK
                     SLIFSFQQNGIYTNAFVNCTLFEDGESYAIEPKTLHFKDAFDQPKGVLSDVIAQNSID
                     VEHLNAYYVTAREITQLIKTQSPTLLDKLTTKQTQSEISQIQNDYISVYDFIEWASKH
                     YSSLSETANDLNRLLKDKNIQLYRQYGGISPSIDKDNTNLKAAFDFVAINNGYEKQSS
                     SFNFDDDIPF"
     gene            complement(2424..2747)
                     /locus_tag="PXA33_001194"
     CDS             complement(2424..2747)
                     /locus_tag="PXA33_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative addiction module antidote protein"
                     /protein_id="HDL5120530.1"
                     /translation="METIQINEQAAEKHGLRPFDVAEFLTDEATIQAYLAEVLKEGDQ
                     DEFLEALNDVARARGMAELAKQTGLGRESLYKTLAKGSKPRFDTIQKILGAFNLVLIP
                     QVKNG"
     gene            complement(2860..3078)
                     /locus_tag="PXA33_001195"
     CDS             complement(2860..3078)
                     /locus_tag="PXA33_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module killer protein"
                     /protein_id="HDL5120531.1"
                     /translation="MMREQTTNQIIITDNFSKWLENLKNPIAKATVARRIKNAINGNF
                     GDHKSLAGTGGLYEMRIATGAGYRIYYA"
     gene            complement(3353..4579)
                     /locus_tag="PXA33_001196"
     CDS             complement(3353..4579)
                     /locus_tag="PXA33_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL5120532.1"
                     /translation="MARKITPLNDTQIRKAKPEDSPLRDGNGLLLVITSNSKLWRFRY
                     ERPFTKKRNDLSIGAYPDISLAQAREIREEYRALLAQNIDPQTHRQQQEQAKANEISN
                     TYENVAWAWLEHRKTKKNFSDNYQKDVIALIKNNLLPHFGRMPITQITAPMAIKAFKQ
                     YQDEGKLEKLKRAIQKHNEIMMFAVHREMIPFNPTANIAKEFDSPTVEHFKTIKPEDL
                     GEFLYTLQNAQIHLQTRYLILWQLLTMTRPNEAAKAKWADIDEKAKLWTIPAENMKRG
                     IEHKITLSRQAIALLREIKKLSGGKTYLFPSVKNPQTHVNTQTANGAIKRMGYKGKLV
                     AHGLRAIASTYLNEKGYNSDLIEVALSHLNSDRIKAAYDRGERLEQRFKLLQVWADFV
                     EECSQGSLPQYHLKIA"
     gene            complement(4854..5220)
                     /gene="ssrA"
                     /locus_tag="PXA33_001197"
     tmRNA           complement(4854..5220)
                     /gene="ssrA"
                     /locus_tag="PXA33_001197"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            5386..6345
                     /gene="rpoH"
                     /locus_tag="PXA33_001198"
     CDS             5386..6345
                     /gene="rpoH"
                     /locus_tag="PXA33_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HDL5120533.1"
                     /translation="MKKFNENEIEDAEIIDAEPIDVDEIDEEETSVEAMHPALVSGSM
                     PVPQGNLDSYIRMANQYPILTAEQEKELAERYYYDEDVEAAKQLILSHMRFVIHIARG
                     YLGYGLPLADLIQEGNIGLMKAVKRFDPNVGVRLVSFAVHWVKAEIHEYVLKNWRIVK
                     VATTKAQRKLFFNLRKNKNRLAWFNEEEIKKVADDLGVSVEEVREMESRMTGQDLGFD
                     LPAGDDDEDSYAPSMYIEDSSSNFADDLEDEQHNGQATAQLAYALATLDERSQDIIKT
                     RWLDDNKATLQDLADKYNISAERVRQLENAALKKIKEAISLDG"
     gene            6511..6804
                     /locus_tag="PXA33_001199"
     CDS             6511..6804
                     /locus_tag="PXA33_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3144 domain-containing protein"
                     /protein_id="HDL5120534.1"
                     /translation="MTQLSTSVYQRADGFINVANAHLKNIAPNQVSNAMLFGCARFNA
                     YVAASKAEYKQQLADSREEVIQYFVEQYKEMLTANLDEYIQNFERYIEGKKAD"
     gene            complement(6822..7124)
                     /locus_tag="PXA33_001200"
     CDS             complement(6822..7124)
                     /locus_tag="PXA33_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HDL5120535.1"
                     /translation="MNTYNNTLNNKNLEKKQLLMAIQAIPASQDFIWDGKDDDERPVT
                     PSEFSIAMKKRGRPAGSKKELVSLRLDKSVLEAFRASGKGWQSRINQVLSDFVKQV"
     gene            complement(7096..7386)
                     /locus_tag="PXA33_001201"
     CDS             complement(7096..7386)
                     /locus_tag="PXA33_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HDL5120536.1"
                     /translation="MKIYTWDETKRKINLAKHGLDFADADEVLSSPYRFDVEVIRNNE
                     FRIQSFAYSFGRLLVLSLAYTEREDYIRVISFRRASKAEREAYYEYLQQYFE"
     gene            complement(7524..8063)
                     /locus_tag="PXA33_001202"
     CDS             complement(7524..8063)
                     /locus_tag="PXA33_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HDL5120537.1"
                     /translation="MLDLTKIKLVITDVDGVLTDGGMYYTDQGEVMKRFHVHDGLGVK
                     MLQSCGIKVAVLSGGDTALLRKRLEVLKIDLALLGKMEKRSACFELMQQAGVTPEQTA
                     YIGDDTLDLPAFEVCGIAIATRNALDYIKEKADWVLQKAGGEGAFREVSDQILEAQGF
                     GEIFKTADGFLTVAGKMAQ"
     gene            8134..8811
                     /locus_tag="PXA33_001203"
     CDS             8134..8811
                     /locus_tag="PXA33_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HDL5120538.1"
                     /translation="MSIAENLSQIHQQIQQISEQYQRDNVRLLAVSKTKPVEAIQAAI
                     EAGQRAFGENYVQEAVEKIEFFANRPELEWHFIGPLQSNKTKLVAAYFDWIQTVDRLK
                     IAERLNEQRPADKAPLNVLIQINISDEASKSGIEPDEMLPLAQAISQLPNLKLRGLMA
                     IPKPESEPEQQKVAFRKMQLLFNRLQTEFDDIDTLSMGMSDDMQAAIECGSTMVRIGT
                     AIFGARR"
     gene            8996..10843
                     /gene="typA"
                     /locus_tag="PXA33_001204"
     CDS             8996..10843
                     /gene="typA"
                     /locus_tag="PXA33_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HDL5120539.1"
                     /translation="MQNVDISKLRNIAIIAHVDHGKTTLVDKLLQQSGTFGETRGDVD
                     ERVMDSNDLEKERGITILAKNTAINWNGYHINIVDTPGHADFGGEVERVLSMVDSVLL
                     VVDAFDGPMPQTRFVTQKAFAHGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLGASD
                     EQLDFPIIYASALNGVAGLEHEDLAADMTPLYEAIVKHVQPPQVELDAPFQMQISQLD
                     YNNYVGVIGIGRIKRGSVKPNQAVTIVDSEGKTRQGRIGQVLGHLGLQRFEATEAFAG
                     DIVAITGLGELNISDTICDINAVEALPALSVDEPTVTMFFCVNTSPFCGQEGKFVTSR
                     QILERLNKELVHNVALRVEETSNPDEFRVSGRGELHLSVLIENMRREGYELAVSRPKV
                     IYREEDGKKQEPFEQVTIDIEEQHQGSVMEALGIRKGEVRDMIPDGKGRTRLEYVIPS
                     RGLIGFRNEFMTMTSGTGLLYSTFSHYDDVKPGEIGQRKNGVLISNATGKALAYALFG
                     LQERGKLMIDHGVDVYEGQIIGIHSRSNDLTVNCLQGKKLTNMRASGKDDAIVLTTPV
                     RFTLEQALEFIDDDELVEVTPQSVRIRKRLLTENDRKRASRTTTSTSTH"
     gene            complement(10892..11329)
                     /gene="rimI"
                     /locus_tag="PXA33_001205"
     CDS             complement(10892..11329)
                     /gene="rimI"
                     /locus_tag="PXA33_001205"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDL5120540.1"
                     /translation="MIEQIQAEDFEQLFEIEQQAHLVPWSKGTLLNNQGEKYLNLKVV
                     EQNKIVAFAISHIVLDEATLFNIAVAPDFQGKGIGKRLLSELILQLQQRKLTTLWLEV
                     RESNHSARKLYDSLGFNEVTTRKNYYPTPNGSKENAVVMALYL"
     gene            complement(11322..11744)
                     /locus_tag="PXA33_001206"
     CDS             complement(11322..11744)
                     /locus_tag="PXA33_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HDL5120541.1"
                     /translation="MNRRDLLLQEMGISQWVLTNPQVLKGDAQIRLNNAVKLVVVCEE
                     DHQHSRLFADILRALKLKNSEYQWLTGEQSQRLVFEHSPIFWLIQAEEQAVKMAKSFT
                     NQTAWKNESWQDLAKSTTKRQLWQQIDTFSAQFGEQRD"
     gene            11874..12863
                     /gene="rsmC"
                     /locus_tag="PXA33_001207"
     CDS             11874..12863
                     /gene="rsmC"
                     /locus_tag="PXA33_001207"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HDL5120542.1"
                     /translation="MLSLESEVLERHLALFENKSVLLFGDVRDDFARQLQSRAEKVAV
                     FSSYFDYARHHSEVEFGLECGLSAELAVFYWTKNKQECQFQLLQWLSQCQAGQEMLII
                     GENRAGVRSVEKLLEPFGNIAKIDSARRCGLYHFELQTVPTFDCKKFWKSYRLQDLDV
                     FDLPAVFSSAELDNGTKLLLSTFDKADRLKGKVLDLGCGAGVIGATLKQQFPKIKLTM
                     SDIHAMALESGRRTLAENQLEGEVVASDVFSHINERFDLIVSNPPFHDGVDTAYRAVE
                     ELIFQAKNHLTKGGELRIVANAFLPYPDLLDKAFGKHDVLAKSTKFKVYSART"
     misc_feature    12993..13115
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            13137..13514
                     /locus_tag="PXA33_001208"
     CDS             13137..13514
                     /locus_tag="PXA33_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor"
                     /protein_id="HDL5120543.1"
                     /translation="MKNPIEIYQSQDGKTQVSVQFDKETVWLSLQQMADIFGRDKSVI
                     SRHLRNIYQEGELEREATVAKNATVQIEGAREVVRLKDYLVKGYALNERRLQERGIEF
                     EQVIGLLSQYTGCLSLVGGRKFA"
     gene            13581..>13934
                     /locus_tag="PXA33_001209"
     CDS             13581..>13934
                     /locus_tag="PXA33_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL5120544.1"
                     /translation="MSINISPLASKNLTEWKLLWQQYLDFYQVNLADDIIQSTWQKVS
                     ESENVKGFGAFVDNRMVGFVHIVIHPNTWNLTECCYLEDLFVLAEYRNQGIGKELIEY
                     IYRYAQEKGYNRVYWV"
BASE COUNT         3937 a         2775 c         2959 g         4263 t
ORIGIN      
        1 gtacgccttt tagtatttgt tgataggctt catcattaat tagggctttt tgaatgaaat
       61 aaccggcttc tatatttgcc ttaatctcat ctaacgaatc gattcttata tggcggtaga
      121 aaataaagct gtcccgtgtg ataaattcgt gtgttcctgc ctttaaaata caagtttcat
      181 caagtgcttt cccgtcttta atgcttgagc agtttacact taaaatcata tctcctttac
      241 cggtttcacc atcaaagcac ggatcggtaa cctgcttatc cagccctagc aacttcgctt
      301 tgcccattgt ggcttgagtc attgcgttag ggttttcttt ctctctcgcc agtatacgag
      361 cctcttccag ctcctttaaa aggtcatcaa cggttaattt atggcgttct gcgtgttcgg
      421 ctttcagttc ctcaatcctt gccgtaatct tgccgttatc cattagctct ttagcttttc
      481 ggtggacggt ttcggacttc atatcttcgg tttcataggc ttggcggtag gcttcgctag
      541 cattgccggt ttcaatataa gcaaagcaaa aagcctcttg ttttggtgtg agttcggtca
      601 ttctttcccc tccgccattc gcttttcagc aaaggcataa tcgatttttt ctcggtattc
      661 tttgctcatc tgctcccgtg taatttcgtt gccgtgtcgg tcaataaatg ggtttgttcg
      721 ttgtgggtgg gggagcttgt tgatttcact caataagtca tcattcacta atagcggcgt
      781 ggcggttagc tgttgatgat aggcaataat cgctagagcc tctaaaaagc ccgtagcccc
      841 tgccatttca atattgatat gatcttcaag gtcaagagaa agcacaccga taaaaaacgg
      901 tctgccgtta tcgtggtgca gtcgttcaat tcggatatgc ggaaaatcta aaaagctcaa
      961 ggttgctttg ataggttcat tcattaaagc agtaataccc gctaaattaa gctcgtgttt
     1021 cattttggtt tattccttta ttctttccgc cacttttaac agttggctaa gttgttttct
     1081 taaaatgccg agtgtgattg aatcggtttc gcttggcatt gcgttatgtt gttgttccag
     1141 cttcgctatt cggttgccta gcacaattaa aagctcgggc aatgggtaag ctcggtttca
     1201 gaatagaggc ttaaaaacgt gaatagtctg ctaaataatc aggtgttatc tcagaaaaat
     1261 cgagcttttt ccctagtgct gcttctaatt cttgtttatt taaaagtaaa ttttggaaat
     1321 catttaaatt cattgctgcc acctagaacg gaatatcatc atcaaaatta aagctgcttg
     1381 attgtttttc atatccgtta ttaattgcta caaaatcaaa ggctgctttt aaattagtat
     1441 tatctttatc aatgcttgga cttattccgc catattgtcg ataaagctgt atatttttat
     1501 ctttgagtaa gcggtttaaa tcgttagctg tttcactaag acttgagtaa tgtttacttg
     1561 cccattctat gaagtcataa acagaaatat aatcattttg aatttggcta atctctgatt
     1621 gtgtttgctt ggtggttaat ttatctaaca aggttggcga ttgtgtttta atcagctgtg
     1681 ttatttctct tgctgttacg taataagcat ttaaatgttc aacatcaata gaattttgag
     1741 caataacatc acttaaaacg cctttaggct gatcgaaagc gtctttgaaa tgtagtgttt
     1801 tgggctcaat agcatagctt tcaccatctt caaagagagt gcaatttaca aaagcattcg
     1861 tatatattcc attttgctga aaggaaaaaa tgaggctttt caatttatcc gctatatgtg
     1921 aaaaaggttt atttgtatag tttgataatt cttgtatcag ttcttgaaag ctgattttgg
     1981 gctcaatacg attttcaagc ctagaaataa attgactgtg atctgccata agcgcccctt
     2041 taacgtatcc ctttactgtg agagcgtgga aagagaaata aagggctttc tcctttcgtg
     2101 ccggttaatt actccgccac tatccacgct aatttgaata aatccgtata gttacggatt
     2161 gaacttgata ggttgtattt tgctaattat tcagataaat tacaagtaat aagctgtgtt
     2221 ttattacagt aaatcaaaat aggctaaaga tgggtttccg gacgctataa aaaagagcat
     2281 tcaagcggtc atatttcatt aaggttttgc gaatagtgcg gaattttcgg agcagtacgc
     2341 acgcaaggag caaataaaaa gccgtcaata tccatcaacg gctatttaaa agtgaggttt
     2401 tgacaggtag caagtgataa gttttagcca tttttgactt gtggaataag cactaaattg
     2461 aatgccccta gaattttttg aatagtgtca aagcgtggtt tgctgccttt ggctagggtt
     2521 ttatataggc tctctctgcc tagccctgtt tgcttggcca gctccgccat tcctcttgct
     2581 cttgctacat cgttcaaggc ttctaaaaat tcgtcttggt cgccctcttt taacacttcc
     2641 gctaaatagg cttgaatagt tgcctcatcg gttaaaaact ccgccacgtc aaaagggcgt
     2701 aaaccgtgct tttctgctgc ctgttcattg atttgtattg tttccattgc tttctcctgt
     2761 agtttttaat tcagcccata atgctttcgc tttctctata tcggcttgct gtgttgattt
     2821 atcgccgccg tgtaacaaga tataaattac atttccttgt tatgcgtaat aaattctata
     2881 tccggctcct gttgctatcc gcatttcata aagtccgcct gtaccggcta aagatttatg
     2941 atcgccaaag ttgccattaa tcgcattttt tatccgtctt gctaccgttg cttttgcgat
     3001 agggttcttt aaattctcaa gccattttga aaaattatcg gtaataataa tttgattagt
     3061 ggtttgctct ctcatcattg ccggcttctt attctattta ttgcttattg tatccattaa
     3121 gatacagtga gcaagggttt ttgccattta cccactttta acccacttta cccactgtca
     3181 ttttttcttt atttttcagt tagttataaa aaaataatag tgaaaagtgg gtaaagtggg
     3241 taaaaatcga aaagtttaca gaattttcat caaggggaat tacgggcata aaaaagccgt
     3301 caggcggttt acctaacggc attacaagcg gtcatttttg gcggaaaatt tactaggcta
     3361 ttttcaaatg atattgcggt aagctacctt gcgaacattc ctctacaaaa tccgcccaca
     3421 cttggagcag tttaaaacgt tgctctaggc gttcgcctct atcataggcg gctttgattc
     3481 ggtcgctgtt taaatgcgat aaggcaacct cgattaaatc gctattgtag cctttctcat
     3541 taagataagt ggaggcgatg gcccgcaagc cgtgagccac taatttgccc ttgtagccca
     3601 ttcgtttgat cgcaccgttg gcggtttgag tattgacgtg cgtttgtggg ttcttcacgc
     3661 tagggaataa atacgtttta ccgccactta gctttttaat ctctcgcaaa agtgcgattg
     3721 cttggcgtga gagtgtgatt ttatgctcaa tgcctcgctt catattttcc gccggtatcg
     3781 tccacagttt ggctttttcg tctatatccg cccattttgc cttagccgct tcattcgggc
     3841 gggtcattgt gagtaactgc cacagaatta aatagcgtgt ctgtaaatga atttgggcgt
     3901 tttgtaaggt gtataaaaac tcgcctaaat cttccggctt aatggttttg aaatgttcaa
     3961 ccgttgggct atcaaactct ttcgcaatat tggcggtagg gttaaacggt atcatttcac
     4021 ggtgtacggc aaacatcata atttcattat gtttttgaat tgcccgtttt aacttctcta
     4081 gtttgccctc gtcttggtat tgcttgaatg ccttgattgc cattggtgcg gttatttgtg
     4141 tgataggcat tcttccaaag tgcggcaaga ggttattttt aattaaggcg attacatctt
     4201 tttggtagtt gtctgaaaaa ttcttcttgg tcttgcggtg ttccagccac gcccacgcta
     4261 cattttcata agtgttgctg atttcgttgg cctttgcctg ttcctgttgt tggcggtggg
     4321 tttgagggtc gatattttga gccaataatg ccctatattc ttctctaatt tctctcgctt
     4381 gtgctaaaga aatatcaggg taagccccaa tgcttaaatc attccgcttt tttgtgaacg
     4441 gtctttcata cctaaaccgc cacaatttag aatttgaggt aataacaagt agtaagccgt
     4501 taccgtctct taatgggctg tcttccggtt tcgcttttct tatttgggta tcgtttaacg
     4561 gtgtgatttt tctagccata gtaaaccttt aggagtataa agcctaaatc ttaggagtat
     4621 gattttttgt ttgggagtac aaaactaaaa gcgataaagc cttatttgaa aaggctctaa
     4681 agtggtttta ggagtacgaa tttttggggg tattccggcg tactcctaaa tatactccta
     4741 aaatcttcgg atttgattgt atcgtatagg tacgcaacgg acaataaaaa agccttgaaa
     4801 cattgatttt caaggctttt taggtatcgg ttgaaccgta taggattata atttggtgga
     4861 gctgggggga tttgaacccc cgtccgaaat ttctctacct tcagtactac atgtttagtc
     4921 tagtctttaa tttcgcttat tcacagcgga cagacacgca atgaataaac tagctttatt
     4981 taatttagtg tttcgatcct aaagcggcgg cgtccacacg atctcgtttt gggtgactta
     5041 tctttgccct cgtcttacga gcggaagctg aggagataag gctttaaagc aggttattaa
     5101 gctgctaaag cgtattgttc gtcgtttgcg actattattt tgcggtttgt ttacgaggcc
     5161 taccgcacct cgacatgcac cttgggcttc gccaatcccg tcgaatccaa aatcagcccc
     5221 aaaagtagcg gtattctaaa ggaaagcggt cgaatttgca aaaagttttg caagtggcgg
     5281 aacttagcgg tattgaatag ctcgaaataa aatcatacaa atcccacacc agatagattt
     5341 aactctgtta taatgtaaac cttgctgaga gtgaagagaa atagaatgaa aaaatttaac
     5401 gagaacgaga ttgaagatgc tgaaattata gatgccgagc cgattgatgt tgatgagata
     5461 gacgaagaag aaacatcggt agaagcaatg catccggctt tggtttccgg ctcaatgcct
     5521 gtgccgcagg gcaatttgga tagctacatc cgaatggcaa atcagtatcc gattctcacc
     5581 gccgagcaag aaaaagaact ggcggagcgt tattattatg atgaagacgt tgaggcggca
     5641 aaacagctga tcctttccca tatgcggttt gtgatccata ttgctcgtgg ctatttgggc
     5701 tatggtttac cgctggcaga tctgatccaa gagggtaata tcggcttgat gaaagcggta
     5761 aaacggtttg acccgaatgt cggcgtgcgg ttggtttctt ttgcggttca ttgggtgaaa
     5821 gcggaaattc acgaatatgt gctgaaaaac tggcgaattg tgaaagtggc aaccaccaaa
     5881 gctcaacgta aattattctt taatttacgc aaaaataaaa accgcttggc gtggtttaat
     5941 gaagaagaga tcaaaaaagt tgccgatgat ttaggggttt cagtggaaga agtacgagaa
     6001 atggaatctc gaatgaccgg acaagattta ggttttgacc tgcctgccgg tgatgacgat
     6061 gaagacagct acgctccttc tatgtatatt gaagatagca gctccaattt cgccgatgac
     6121 ttggaagacg aacaacataa tgggcaggca accgctcaac ttgcctatgc gttagccacc
     6181 cttgatgaac gtagccaaga catcatcaaa acccgctggt tagatgacaa taaagccaca
     6241 ctgcaagatt tagccgataa atataacatc tcggcagagc gggtaagaca gcttgaaaat
     6301 gccgcattga agaaaatcaa ggaagcgatt tcattagacg gctagaaatg cctacaagcg
     6361 gtgatttttt tactcgctac gctcgcactt cgtgccgtcg gcagagccaa cgttcaaacg
     6421 caagcgtttg tgcaaaattt tacgcaaaaa gcaccgcttg tattttgact aaaaatgagg
     6481 atactatgat taacattacc gataaacaaa ttgacccaac tttctaccag cgtctaccag
     6541 cgagcagacg gctttattaa cgttgccaac gctcatttaa aaaacattgc cccaaaccaa
     6601 gtcagcaatg cgatgttgtt tggttgtgcc agatttaacg cctatgttgc ggcaagcaag
     6661 gcggaatata aacagcagtt ggccgattcc agagaagaag ttattcaata ttttgtggag
     6721 caatacaaag aaatgctcac tgcaaattta gatgaatata tccaaaactt cgagcgatat
     6781 attgaaggga aaaaggcgga ttaaccgcct ttgttttgtg gtcaaacttg ttttacaaaa
     6841 tcacttaaaa cttgattaat tctactttgc caacctttac ctgaagctct aaaggcttca
     6901 agaacgcttt tatcaagacg taacgaaacc aattctttct ttgaaccagc tggtctgcca
     6961 cgttttttca ttgcaattga aaactctgat ggcgtaaccg ggcgttcatc gtcatctttt
     7021 ccgtcccaaa taaaatcttg gcttgccgga atagcttgaa tagccataag tagctgtttt
     7081 ttctctaaat ttttattatt caaagtattg ttgtaagtat tcataatatg cctctctttc
     7141 tgctttagat gctcgccgaa agctaattac tctgatgtaa tcttcccttt ctgtatatgc
     7201 aagtgataat acgagtaaac gtccaaatga atatgcaaaa gattgtattc taaattcatt
     7261 gttacgaata acttcaacat caaatcgata gggcgatgag agaacttcat ctgcatcggc
     7321 aaaatctaat ccgtgcttgg ctaaattgat tttgcgttta gtttcgtccc aagtataaat
     7381 tttcataatt attgtatata cataaaataa atctaacaag aactaaattt aatttagcaa
     7441 acagtaattt gaaaatcaga ttagaaatac attttttcga tgcagatcgc aaaaataaaa
     7501 tgaaatttaa ctgcttgaag agtctactga gccattttcc ccgctaccgt taaaaaacca
     7561 tctgcggttt taaaaatttc accgaatcct tgagcctcca aaatttggtc ggagacttca
     7621 cgaaacgccc cttcaccacc ggctttttgc aatacccaat ctgctttttc tttgatgtaa
     7681 tcgagggcgt tacgggttgc aatcgcaatg ccacacactt caaaagccgg taaatctaag
     7741 gtgtcatcac caatgtaagc cgtttgctca ggggtaacac cggcttgctg cattagctca
     7801 aagcaggcgg agcgtttttc cattttgcct aacaaagcga gatcaatttt cagcacctct
     7861 aaccgtttgc ggagcaaagc cgtatcgccg cccgagagta ctgccacttt aatgccacaa
     7921 ctttgtaaca tcttaacgcc taaaccatcg tgaacgtgga aacgtttcat tacttcgcct
     7981 tggtcggtgt agtacattcc gccatcggtt agcacgccgt ccacatcggt aatcactaat
     8041 ttaatcttgg tgagatctaa catttttttg cctcaaatat ggtatgattt taccattata
     8101 gcttttttac ctccgtttac caaaggaacc gaaatgtcga ttgccgagaa tttatcgcaa
     8161 atccatcaac aaattcagca aatttccgaa caatatcaac gagataacgt gcgtttgtta
     8221 gccgtctcca aaactaaacc agttgaggcg attcaagcgg cgattgaggc agggcagcgt
     8281 gcatttggcg aaaattatgt gcaggaagcg gtcgaaaaaa tcgaattttt tgcaaatcgc
     8341 cccgaattag aatggcattt tattgggcca cttcaatcaa ataaaaccaa attggtcgct
     8401 gcctattttg attggattca aacggtggat agattaaaaa ttgccgagcg tttaaacgaa
     8461 caacgcccag cggataaagc cccgctcaac gtgctgattc aaattaatat cagcgatgaa
     8521 gcctcgaaat cgggcattga accagatgaa atgctaccac ttgcccaagc gatttctcaa
     8581 ttaccgaatt tgaaactgcg gggcttaatg gccattccca aaccagaaag cgagccagag
     8641 caacaaaaag ttgcctttcg caaaatgcag ctacttttta accgcttgca aacagaattt
     8701 gacgacattg ataccctttc aatggggatg tcggacgata tgcaagcggc gattgaatgt
     8761 ggctcgacaa tggtgcggat tgggactgcg atttttgggg cgagacgtta gccagctgag
     8821 tttttacata aagctgatac aagcggtcaa attcgccaaa aactttgcaa attccaccgc
     8881 ttgttttgta gccaacagcg gaaaaagtct gtataattac gcctcctaat ttcttgactc
     8941 tcgccagttt tgatgggcga gagtttttat tttgacttaa cttaaagata ttccaatgca
     9001 aaatgtagat atttcaaaat tgcgtaacat tgcaattatt gcccacgttg accacggtaa
     9061 aaccaccttg gttgataaac ttttacaaca atcaggcacc tttggtgaaa ctcgtggtga
     9121 cgttgatgag cgtgtaatgg attccaacga tcttgaaaaa gagcgtggca ttaccattct
     9181 tgcaaaaaat acggcaatta actggaatgg ttatcacatc aatatcgtag ataccccagg
     9241 gcacgctgac ttcggtggtg aggttgagcg tgtactttca atggtagatt ctgttctgtt
     9301 agtggttgat gcctttgatg gcccgatgcc gcaaacccgt ttcgtcacgc aaaaagcgtt
     9361 cgctcacggt ttaaaaccaa tcgtagtgat caataaagtt gaccgcccag gtgctcgccc
     9421 tgattgggtg gtggatcaag tgttcgactt gtttgtaaat ttaggtgcaa gtgatgagca
     9481 gttagatttc ccaattatct acgcttcagc cttaaatggt gtagcaggct tagaacacga
     9541 agacctagcg gcagatatga ccccacttta tgaagcgatt gtcaaacacg ttcaaccacc
     9601 acaagtggaa ttagatgcac cattccaaat gcagatttcg caattagact acaacaacta
     9661 tgtgggcgtt atcggtattg gacgtatcaa acgtggttcg gtgaaaccta accaagcggt
     9721 aacgattgta gatagcgaag gcaaaactcg ccaaggtcgt atcggtcagg tgttggggca
     9781 tttaggttta caacgttttg aagcaacaga agcctttgct ggcgatattg ttgcgattac
     9841 tggtttaggc gaattaaata tttcagatac tatttgtgat attaacgcag tagaggcttt
     9901 accagcatta agcgttgatg aaccaacggt aacgatgttc ttctgcgtaa acacctcgcc
     9961 attctgcggt caagaaggta aatttgtgac ttcacgccaa attcttgagc gtttaaacaa
    10021 agaattagtc cacaacgtgg cattgcgtgt ggaagaaact tcgaacccag acgaattccg
    10081 tgtgtcaggt cgtggcgaat tacacttatc ggtattaatc gaaaatatgc gtcgtgaagg
    10141 ttacgagtta gcagtgtctc gcccgaaagt aatctaccgc gaagaagacg gcaaaaaaca
    10201 agagccgttt gagcaagtga cgattgatat cgaagagcaa caccaaggtt cagtgatgga
    10261 agcacttggt atccgtaaag gtgaagtgcg tgatatgatt ccggacggca aaggtcgtac
    10321 ccgtttagag tatgtgatcc caagccgtgg cttaatcggt ttccgtaacg aatttatgac
    10381 gatgacctca ggtactggtt tactttactc tacattcagc cactatgatg acgtaaaacc
    10441 gggcgaaatc ggtcagcgta aaaacggtgt gttaatttct aacgcaacgg gtaaggcatt
    10501 agcatacgca ttatttggct tacaagagcg tggaaaatta atgatcgacc acggtgttga
    10561 tgtatatgaa gggcaaatta tcgggattca cagccgttca aacgatttaa ccgtaaactg
    10621 cttacagggt aagaaattaa ccaatatgcg tgcatcaggt aaagacgatg ccatcgtttt
    10681 aaccacaccg gtgcgtttca ctctagaaca agcgttagag tttattgacg atgacgaatt
    10741 agtggaagta acaccacaat cggtgcgtat tcgtaaacgt ttattaactg aaaacgaccg
    10801 gaaacgtgca agccgtacta caaccagtac cagcacgcac taactgataa aaaagagcga
    10861 ctgaggtcgc tcttttggtt ttaaattctc cctacaaata taacgccatt accaccgcat
    10921 tttctttgct accgtttggc gtggggtaat agtttttgcg ggtggtaact tcattaaaac
    10981 cgagtgaatc gtagagtttt ctggcagaat ggttggattc tcgcacttcc aaccacaggg
    11041 tggtgagttt ccgttgttgc agttgcaaaa ttaattcgga aagtaaccgc ttgccaatgc
    11101 ctttgccttg aaaatcagga gcaactgcaa tgttaaataa ggttgcttca tctagcacga
    11161 tatgactaat ggcaaaagcc acaattttgt tttgctctac cacttttaaa ttcaggtatt
    11221 tttcaccctg attatttaat aacgtcccct ttgaccacgg cactaaatgg gcttgttgct
    11281 caatttcaaa taactgctcg aaatcttccg cttgaatttg ctcaatcacg ctgttctcca
    11341 aattgtgccg aaaaagtatc aatttgttgc caaagttggc gtttggtcgt agatttggct
    11401 aaatcttgcc aagactcgtt tttccaagcg gtctgattag taaaactttt tgccattttg
    11461 accgcttgtt cttccgcttg aatcagccaa aagatcggcg aatgctcaaa caccaaacgt
    11521 tgtgattgtt cgccagtcag ccattgatat tcgctgtttt ttaactttaa agcacgcaga
    11581 atatcggcaa agagccggct gtgttggtga tcttcctcac acactaccac caattttacc
    11641 gcattgttta ggcggatttg agcatcgcct tttagcactt ggggattggt tagcacccat
    11701 tgcgaaatac ccatttcctg taacagtaaa tcacgacgat tcatttaaaa cctcatcaaa
    11761 ttttgcaaat agtatcaaaa ctccgcttga acagaaactt ttttcaaaaa attcaagatt
    11821 tctgtactta aattcggtat attcacgcta aattttttag ataaggtaag aaaatgctct
    11881 ctttagaaag cgaagtactt gaacgccatt tggcgttatt tgaaaacaag tcggtgttat
    11941 tatttggcga tgtgcgagat gattttgccc gccagctgca atctcgtgcg gaaaaggttg
    12001 cggtattcag tagctatttt gattacgcac gccaccatag tgaggttgaa tttggcttag
    12061 aatgtggttt gagtgccgag ttagcggtgt tttattggac gaagaacaag caggaatgcc
    12121 aattccaact gctacaatgg ctgtcgcaat gccaagcagg gcaagaaatg ttgattatcg
    12181 gcgaaaaccg agctggtgtt cgttcggtag aaaaactgct cgaaccgttc ggcaatattg
    12241 ccaaaatcga ctctgcacgc cgttgtggtt tataccattt tgagctacag accgtgccga
    12301 cattcgattg caaaaaattc tggaaatcct accgcttgca agacttagat gtgttcgatc
    12361 taccagcggt ttttagctcg gcggagctgg ataacggcac caaattattg ctttcaacct
    12421 tcgacaaagc agacaggcta aaaggcaaag tgttagattt aggctgtggg gctggcgtga
    12481 ttggggcaac gctcaaacaa caatttccga aaatcaaact gacgatgagc gatattcacg
    12541 caatggcgtt ggaatccggc agacgtactt tggcggaaaa tcagttagag ggggaagtgg
    12601 tagcaagtga tgtgttctct cacattaatg aacggtttga tttaattgtc tccaacccac
    12661 cattccacga cggggtcgat accgcctacc gagcggtgga ggagttgatt ttccaagcta
    12721 aaaatcacct aaccaaaggc ggtgagctgc gaattgtcgc aaacgcattc ttgccttacc
    12781 cagatttgct cgataaagcc ttcggcaaac acgacgtact ggcaaaatcc accaagttta
    12841 aggtgtattc cgcaagaaca taataggcag aacaaacggt tataaaaaag ccggtatttg
    12901 cttaaaaaag tgcttgatat ttctgccggc ttgacgtaaa ttctgttcat actgttttgg
    12961 agtaatgcaa tgaatttaaa ctacacttta catcaccacc acactttaac ctgatatctt
    13021 tcgggcattt ggtgtgtgcg gaagataact cttccgagca agtgtagcaa atcaataaag
    13081 atctctcgga aggcaacttt cgagagattt ttttatagat aaaatttggg aataatatga
    13141 aaaacccgat tgaaatttac caaagccaag acggcaaaac ccaagtttcc gtgcagtttg
    13201 ataaggaaac ggtgtggcta agtttacagc aaatggcaga tatttttgga cgagataagt
    13261 ctgttatttc tcggcactta cgaaatatct accaagaagg tgaactggag cgtgaggcaa
    13321 ctgttgcaaa aaatgcaaca gttcaaattg aaggtgctag agaagtcgtt cgcctaaaag
    13381 actatctggt gaaaggctat gcccttaacg aacgccgttt gcaggaacga ggtatcgagt
    13441 ttgagcaggt gattggtttg ctcagtcaat acactggttg tcttagcctt gttggtggca
    13501 gaaagtttgc ctgaacaaaa agaactgatg attaaactga ttgaacactt tattttatta
    13561 aaagcataac aggaaaaaat atgagtatta atatttcgcc gttagcctcc aaaaatctca
    13621 ctgaatggaa actattgtgg caacaatatt tagattttta tcaagtgaat ttagcagatg
    13681 atattattca atcaacttgg cagaaagttt cagagagtga aaatgtaaag ggatttgggg
    13741 cttttgttga taacagaatg gttggttttg ttcatattgt gattcaccca aatacttgga
    13801 atttaactga atgctgttat ttggaagatt tatttgtttt agctgaatat cgcaaccaag
    13861 gcataggaaa agaattaatt gaatatattt atcgatatgc acaggaaaaa ggatataacc
    13921 gagtatattg ggta
//
LOCUS       DANLRK010000058        13822 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.058, whole genome shotgun sequence.
ACCESSION   DANLRK010000058 DANLRK010000000
VERSION     DANLRK010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 13822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13822
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.058"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(88..804)
                     /gene="rph"
                     /locus_tag="PXA33_001210"
     CDS             complement(88..804)
                     /gene="rph"
                     /locus_tag="PXA33_001210"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HDL5120545.1"
                     /translation="MRPNNRANNQVREIKITRNYTRYAEGSVLIEFGETKVLCNATVE
                     ESVPRFLKGQQQGWVTAEYGMLPRATHSRTQREAAKGKQGGRTMEIQRLIARSLRAVV
                     DLKALGERTVTVDCDVIQADGGTRTASITGACVALYDAMNKLVANGTLKTNPMKGLVA
                     AISVGIVAGEAVCDLEYLEDSNAETDMNVVMVEDGRLVEVQGTAEGEPFSHQELLTLL
                     DLAKQGISQLVEAQRKALAE"
     gene            1085..1948
                     /locus_tag="PXA33_001211"
     CDS             1085..1948
                     /locus_tag="PXA33_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011272075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HDL5120546.1"
                     /translation="MIYSMTAFAHLEMKKEWGNAVWEIRSVNQRFLETYFRLPEQFRN
                     LEMTLRERLRSALTRGKVECSLRIDLSKTQNSEIALNKEYAAQVVQSLKWLKETANEG
                     EINLVDVLRFPGVVDNESQDLDQIAQDLLAGFEQILADFIAMRAREGENVQALILQRL
                     EAISVEAAKVQAQMPEILQWQKDRLQQRFDELNLQLDPQRLEQEMVLLAQRVDVAEEL
                     DRLQLHVKETTNILKKGGAVGRKLDFMMQELNRESNTLASKSINADVTNSAVELKVLI
                     EQMREQIQNLE"
     gene            1957..2847
                     /locus_tag="PXA33_001212"
     CDS             1957..2847
                     /locus_tag="PXA33_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein YgfZ"
                     /protein_id="HDL5120547.1"
                     /translation="MAMNFKQVSQSYPNLCVPLSQYCLIEIAGVDAEKYLQGQLTCDV
                     AKISVGEHTLTSHCDPKGKMSALLRLYRAENEKFMAIIHRSLLPEALTQLKKYAVFSK
                     VTFTELDTPLYGMAGEAAFAKLSENMTALRLTTGQPRAIVWGEELETTADEQLWTLMD
                     IQDGIPVLLQQNQFELIPQATNLQALESAISFTKGCYIGQETVARAKYRGANKRALFT
                     LAGNFESEISLPEVASAIEMQLGENWRATGTVLTSATDENTLWVQVVLNKDVEPNTKF
                     RANGVALEMVELPYSLAENE"
     gene            complement(2879..3139)
                     /locus_tag="PXA33_001213"
     CDS             complement(2879..3139)
                     /locus_tag="PXA33_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HDL5120548.1"
                     /translation="MALLITHKCTNCDMCLPECPNEAISVGDDIYIIDPVLCTECVGH
                     YEKPTCQKVCPITNCIIKDPDNIETEEQLWERFVMIHHPDQI"
     gene            3369..3788
                     /gene="rbsD"
                     /locus_tag="PXA33_001214"
     CDS             3369..3788
                     /gene="rbsD"
                     /locus_tag="PXA33_001214"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005753460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HDL5120549.1"
                     /translation="MKKTLNLNAQLSHSIATLGHTDSLTVCDAGLPIPNTIERIDLAL
                     TAGVPSFLQTFDVVVSELFVERVLLAEEIKHKNPQVLAALLERLTQLEQTQQNKIQID
                     YVNHEIFKEQTHYSKAVVRSGECSPYANIILYSGVPF"
     gene            3798..5294
                     /gene="rbsA"
                     /locus_tag="PXA33_001215"
     CDS             3798..5294
                     /gene="rbsA"
                     /locus_tag="PXA33_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HDL5120550.1"
                     /translation="METLLKMSGIDKEFPGVKALSNACLSVYGGRVMALMGENGAGKS
                     TLMKVLTGIYSKDAGSISYLGKEVTFKGPKDSQEAGISIIHQELNLVGNLTIAENIFL
                     GRESKTAWGAIDWKKMYAEADKLLARLGVKHSSHQLCAELSIGEQQMVEIAKALSFES
                     QVIIMDEPTDALTDTETASLFKVIRELKAENRGIVYISHRLKEIFEICDDVTVLRDGQ
                     FIGERVIADIDEDQLIEMMVGRRLEEQYPHLHQERGELLLEVKNLSGSGVNNVSFQLH
                     KGEIVGISGLMGAGRTELMKVLYGALPKTAGSIELNGKAISNHCPQDGLNNGIVYISE
                     DRKGDGLILGMSVKENMSLTFLDHFSKAGSIRHDAEKLAVDDFIEMFNIKTPSREQQI
                     GLLSGGNQQKVAIAKGLMTRPNVLILDEPTRGVDVGAKKEIYQLINEFKKDGLSILMV
                     SSDMPEVLGMSDRILVMHEGQISAEFSRAEATQEKLLAAAIGKSTGLN"
     gene            5304..6272
                     /gene="rbsC"
                     /locus_tag="PXA33_001216"
     CDS             5304..6272
                     /gene="rbsC"
                     /locus_tag="PXA33_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005753457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HDL5120551.1"
                     /translation="MATQTKQFQLGRFLIEQRSFVALFILIVSVSMINPDFFSVDNIL
                     NILRQTSVNAIIAVGMTFVILIAGIDLSVGSVLALTGAVAATLVGSELPILLVIPLVL
                     LLGTAFGGISGAIVAKGKVQAFIATLVTMTLLRGITMVYTDGRPISTGFSDQADAFAF
                     IGTGYLFGIPVPIWIMAVVFGLAWYILKHTPIGRYIYALGGNESATQLSGINVNKIKI
                     FVFAVSGFLSALAGLIVTSRLSSAQPTAGVSYELDAIAAVVVGGTSLMGGKGRVMGTL
                     VGALIIGFLNNALNLLDISSYYQMIAKALVILAAVLADNYLGTKKV"
     gene            6295..7173
                     /gene="rbsB"
                     /locus_tag="PXA33_001217"
     CDS             6295..7173
                     /gene="rbsB"
                     /locus_tag="PXA33_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006995182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HDL5120552.1"
                     /translation="MKKLTTIASAVMLSLSVAATASAKETIALAVSTLDNPFFVSLKD
                     GAQKKADELGYKLVVLDSQNDPAKELSNVEDLTVRGAKVLLINPTDSEAVGNAVAIAN
                     RNKIPVITLDRGAAKGEVVSHIASDNVAGGKMAGDFIAQKLGSGAKVIQLEGIAGTSA
                     ARERGEGFKQAIEAHKFNVLASQPADFDRTKGLNVTENLLSSKGDVQAIFAQNDEMAL
                     GALRAVSSAKKEVVIVGFDGTDDGVKAVESGKLAATIAQQPELIGSLGVETADKVLKG
                     EKVEAKIPVDLKVISK"
     gene            7294..8223
                     /gene="rbsK"
                     /locus_tag="PXA33_001218"
     CDS             7294..8223
                     /gene="rbsK"
                     /locus_tag="PXA33_001218"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HDL5120553.1"
                     /translation="MKKLTVLGSINADHVISVPYFAKPGETLTGHSCHIAYGGKGANQ
                     AVAAARLGAKVSFIGCIGSDDIGKAMKSAFEQDGIDTRSIKTIPNEMTGIAMIQVAES
                     GENSIVISAGANGHLDETVVAKCAAEITQADYLLMQLETPLPAIVQAAKLAQQNGTKV
                     ILNPAPARALPDELLNLLDMITPNETEAEILTGVKVVDEASAIQAAQVFHQKGIEKVL
                     ITLGSKGVFVSENGVGKIVAGFRVQAVDTTAAGDTFNGALVTAMLEDKPLDQAIRFAH
                     AAAAISVTRKGAQPSIPTRQETLDFLSQQAVSF"
     gene            8237..9247
                     /locus_tag="PXA33_001219"
     CDS             8237..9247
                     /locus_tag="PXA33_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDL5120554.1"
                     /translation="MATMKDIARLAQVSTSTVSHVINNSRFVSEEIRERVQRVVKELN
                     YTPSALARSLKVNETKTIGMLVTATGNPFFAEVMAGVEQYCQQHQYNLIIATTNGDTE
                     RLQHHLQMLMQRKVDGLLLMCGDTRLNADESLNIPLPLVVLDWWFTELNADKIFENSI
                     SGGYLATKTLIEAGHSKIAIITGNLKKSLAKNRLEGYKKALAEANIPLNPNWVFESHF
                     DFAGGVNGMNALLAQSEKPTAVFACSDTIALGAYQAIWQQGLRIPQDISIIGYDDIEL
                     AKYLSPPLSTISQPKAELGRLAVETLLKRIRFKSTDFQTIMLEPELVLRHSIQTINKN
                     AE"
     gene            complement(9283..9912)
                     /locus_tag="PXA33_001220"
     CDS             complement(9283..9912)
                     /locus_tag="PXA33_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005622307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDL5120555.1"
                     /translation="MTAPTQILLIDDHPLMRQGMRQLLELDHQFSVVGEASNGTDGIK
                     LALRLEPDVIILDLNMKGLSGLDTLRSLRAQGIDARIIVLTVSDEQSDVFALMDAGVD
                     GYLLKDTDTPELLDNIRKAAAGEVVLSETVRQHLLNRQPENDPLSCLTDRELDVLQWL
                     ATGMSNKQIAAQLFISEETVKVHIRNLLRKLNVHSRVAATVLYLEQQKG"
     gene            10110..12269
                     /gene="rlmKL"
                     /locus_tag="PXA33_001221"
     CDS             10110..12269
                     /gene="rlmKL"
                     /locus_tag="PXA33_001221"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HDL5120556.1"
                     /translation="MTNQTTYFATAARGFEEILKTELEQICAAECKVAQGGVHFTTTQ
                     KGAYQALLHSRLASRILLPLISTKIFSDSDLYASIVSFNWADLFDPRDTFYIDFSGTN
                     REIRNTQFGAMRVKDGIVDYFERKGFARPTVDKDNPDVRIHVYLDRENVVVSLDLSGD
                     ALHIRGYREETGKAPLRETLAAAIILRSGWKVGTPLVDPMCGSGTLLIEAAQMAANIA
                     PQLHRKHWGFNAWKGHQQAMWKAVLDEAFRNVELGAVGENCNSSLQKMFFGFDLDHRV
                     LTKAKQNAKNAGVDHLIQWQQGDVAALKNPIPEKIGTVVCNPPYGERLGTTPALIALY
                     SVFGQRLKQQFAGWNASIFSGEPELLNCLRLRSARQFKAKNGPLDCVQKNYSISEHNK
                     RSDLDENLQFEQNAQVAPDFANRLTKNIKKIEKWAKQQGIDAYRLYDADLPEYNLAVD
                     RYGDHIVVQEYQAPKNIDEQKARQRLLDAVSATLYVTGVETNKLVLKVRQKQKGTNQY
                     EKLANKGEYFYVNEYGAKLWVNLTDYLDTGLFLDHRLTRKMVGEMAKGKTFLNLFAYT
                     GSATVHAALNGAKSTTTVDMSNTYLNWAEQNLELNNLSLRHHRLFQADCLQWLAECRE
                     RFELIFVDPPTFSNSKRMENSWDVQRDHIELMKQLKRILTADGTIVFSNNKRGFKMDF
                     DGLAALGLTAENISHKTLPLDFERNPQIHNCWIVKNV"
     gene            12446..12925
                     /gene="trmL"
                     /locus_tag="PXA33_001222"
     CDS             12446..12925
                     /gene="trmL"
                     /locus_tag="PXA33_001222"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015432117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HDL5120557.1"
                     /translation="MLDIVLYEPEIPQNSGNIIRLCANCGFRLHMIEPFGFGWDDKKL
                     RRSGLDYHEFVDIQKYKNFEDFLERAKPKRLFALTTRGEQNHSDVKYELGDFLMFGPE
                     SRGIPKPLLAEMPMSQKIRIPMCKDSRSMNLSNSVAVVVYEAWRQFGYQNAVAAQSI"
     gene            complement(13019..>13822)
                     /locus_tag="PXA33_001223"
     CDS             complement(13019..>13822)
                     /locus_tag="PXA33_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HDL5120558.1"
                     /translation="LFEQKSVRSHYDEEKDKWYFSIIDVIAILTESLDPSAYWRKLKQ
                     RLKAEGNETVTNCHGLKMPSADGKMRLTDVADTEQLFRLIQSIPSPKAEPFKQWLAKV
                     GYERLQEINDPSQSLDRARENWQKMGRSEKWIQQRMMGQETRNKLTDYWKSSGVEQPQ
                     DFAFLTNVIHQEWSGLSVQKHKSLKGLKSQNLRDHMSEAELLFTALAELSSRQIAESM
                     QAVGVDENAQAGKQGGNIAKNARQELENKTGKSVVTGENFLPPAKNEEE"
BASE COUNT         3991 a         2711 c         3209 g         3911 t
ORIGIN      
        1 aaaaaaatct acatctgaat cagaacggtt attctgtttt gtcagatgta gattttattt
       61 aaaaagaaaa tcaaaaagag aagtgaatta ttcagctaac gctttacgtt gagcttcaac
      121 tagctggcta atgccttgct tcgctaaatc tagcaatgtc agtaattctt ggtgcgagaa
      181 cggttcgcct tctgccgtgc cttgtacttc cactaaacga ccatcttcga ccatcaccac
      241 gttcatatca gtttcagcgt tggaatcttc cagatattct aaatcgcaca ccgcttcgcc
      301 tgctacaata ccgacagaaa ttgctgcgac taaacctttc attgggttag tttttagtgt
      361 tccattagct actaacttat tcattgcatc gtacaatgct acgcacgcac cggtaattga
      421 ggcggtgcga gtgcctccat cggcttgaat aacatcacaa tctacggtaa cggtacgttc
      481 gcctaaggct tttaaatcta ccaccgcacg cagagaacgg gcaatcaagc gttggatttc
      541 cattgtgcgt ccgccttgtt tgccttttgc cgcttcacgc tgagtgcgag agtgggtggc
      601 tcgtggcaac ataccatatt ctgcggtaac ccaaccttgt tgctgacctt ttaaaaagcg
      661 tggcacgctt tcttctaccg tagcgttaca caatactttg gtttcgccaa actcaattaa
      721 taccgagcct tcggcatagc gagtgtagtt acgggtaatt ttaatttctc taacttggtt
      781 gtttgctcga ttatttggac gcattttctt ttccttttgc taaaattttt accattttag
      841 caaagaacac agtaaaatgt gatttttctt tagcaaagaa tttacatagc cctcgcccct
      901 tgtgggagag ggacggattt ttcttcgttc agaagaaaaa tcagggagag gggtaagcgg
      961 ttatttttat ctcaaatttt acaaattcct ccctctctct gaaaaatatt tctgaacgaa
     1021 atatttttcg gtctctctcc ctcaagggga gagagcttta cttataaaaa cacaaggaat
     1081 accaatgatt tatagtatga cagcatttgc ccatttagaa atgaaaaaag agtggggcaa
     1141 tgcggtgtgg gaaattcgct cggtaaatca acgcttttta gaaacctatt ttcgtttgcc
     1201 ggagcagttt cgcaacctag aaatgacctt gcgtgagcgt ttacgttctg cactgacacg
     1261 aggcaaagta gagtgtagct tacgcattga tttaagcaaa acccaaaaca gtgaaattgc
     1321 gttaaacaaa gaatatgccg cacaggtggt tcaatcatta aaatggttaa aagaaaccgc
     1381 aaatgaaggc gaaatcaacc ttgttgatgt gctacgtttc ccgggcgtgg tggataatga
     1441 aagccaagat ttagaccaaa ttgctcaaga tttattagcc ggctttgaac aaattctggc
     1501 agattttatc gcaatgcgag ccagagaggg cgagaacgtg caagccctga tcttacaacg
     1561 tttagaagca atttcagttg aggctgcaaa ggtacaagcc caaatgccgg aaattctgca
     1621 atggcagaaa gatcgtttac aacaacggtt cgatgaactg aatttacagc ttgatccaca
     1681 acgcttagaa caagaaatgg tgttacttgc ccaacgtgtt gatgtggcgg aggaattaga
     1741 ccgcttgcaa ttacacgtta aagaaaccac caatatcctg aaaaaaggcg gtgcggttgg
     1801 gcgtaaactg gattttatga tgcaagaact aaaccgtgaa tccaacacgc tcgcctcaaa
     1861 atcgattaat gcggacgtga ccaattcggc ggtggagctg aaagtgttga tcgaacagat
     1921 gcgtgaacaa attcagaatt tagaatagga cagattatgg caatgaattt caagcaagtg
     1981 agccagagct atccaaatct ctgcgtgccg ctttctcagt attgtttaat tgaaatcgca
     2041 ggggtagatg ctgagaaata tcttcaaggg caattaacct gcgatgtggc aaaaatttcg
     2101 gtgggcgaac atacgctaac cagccattgt gatccgaaag gcaaaatgag tgcgttactg
     2161 cgtttgtatc gtgcagaaaa tgagaaattt atggcgatca ttcaccgcag cttattgcct
     2221 gaagccctga cgcaacttaa aaaatatgcg gttttttcaa aagtgacctt cacggaatta
     2281 gacaccccac tttatggtat ggcaggtgaa gcagcatttg caaaattaag cgaaaatatg
     2341 accgctttgc gtttaaccac aggacagcct cgggccattg tttggggcga agagcttgaa
     2401 acgactgccg atgaacagct ctggacatta atggatattc aagacggcat tcctgttctc
     2461 ttgcaacaaa accagtttga gctgattcca caggcaacca atttacaggc acttgaatct
     2521 gcgatttcat tcaccaaagg ttgctacatc gggcaagaaa cggtagcgag agcgaaatat
     2581 cgtggggcga ataaacgtgc tttatttacc cttgccggca actttgaaag cgaaatcagc
     2641 ttaccggaag ttgcttctgc gattgaaatg caactgggcg aaaattggcg agcaaccggc
     2701 actgtgttga ccagtgcgac tgatgaaaat accctgtggg tgcaagtggt gctgaataaa
     2761 gatgttgaac ctaacaccaa gtttagagcc aatggtgtag cgttggaaat ggtcgagtta
     2821 ccttatagtt tggcagaaaa tgagtaaaac gaacggtcat cagggtatga ccgttttttt
     2881 atatctgatc agggtgatga atcatcacaa aacgttccca caactgctct tcggtttcga
     2941 tattatcagg atctttgata atgcagttgg taatcgggca aactttctgg caagtcggct
     3001 tttcataatg cccgacacat tcggtacata acaccggatc gatgatataa atatcatcgc
     3061 ccaccgaaat cgcctcgttc gggcattccg gcaagcacat atcacagttg gtacatttgt
     3121 gggtaattaa taaagccata gtttatattg agaaaataaa aattaaacaa gcggtcgatt
     3181 ttgcaaaaaa atttgcaaat cttaccgctt gtcactaaat gctacctatt ataaagcaaa
     3241 ataatttgaa ctacttcaca tttttgcaat ttggttgttt atccgattaa gaaaattttt
     3301 tatgatgcca tcgaaacgtt tcgatggagt attttccaac gtgatggagc gaaaataaag
     3361 gggttagtat gaaaaaaacg ctaaatctaa atgcacaact ttcgcacagt attgcgacac
     3421 tcgggcatac cgatagttta acggtttgcg atgccggttt gccgattcca aacacaatag
     3481 agcggatcga tttggcttta accgccggtg tgccaagttt tttacaaaca tttgatgtag
     3541 tagttagtga attatttgtt gaacgagtgc tacttgctga agaaattaaa cataaaaatc
     3601 cgcaagtgtt agcagctctt ttggagcgtt taacccaatt agaacaaact caacagaata
     3661 aaattcaaat tgattatgtg aaccacgaaa tctttaaaga gcagacacat tacagtaaag
     3721 ctgttgtgcg ttcgggtgag tgttcaccgt atgccaatat tattttatat tcgggcgtgc
     3781 cgttttaggg tggaatgatg gaaaccttat taaaaatgag cgggatagat aaagaatttc
     3841 ccggcgtgaa agccttgagc aatgcgtgct tatctgttta cggtgggcga gtaatggcat
     3901 taatgggcga aaatggggca ggtaaatcca ccttgatgaa agtattgacg gggatttata
     3961 gcaaagatgc cggctctatt agctatttag gcaaagaagt tacctttaaa ggcccgaaag
     4021 attcacaaga agccggtatt agcattattc accaagagct gaatttagtc ggtaatttaa
     4081 ccattgctga aaatattttt ttgggccgtg agtccaaaac cgcttgggga gctattgatt
     4141 ggaaaaaaat gtatgccgag gcggataaac tgcttgcccg acttggggta aaacacagta
     4201 gccatcagct ttgtgcggag ttatccattg gcgagcagca aatggtggaa attgcgaaag
     4261 ccctgagttt tgagtctcaa gtaattatta tggacgaacc gaccgatgct ttaaccgata
     4321 cagaaacagc atcgttgttt aaagttattc gagagttaaa agcggaaaat cgaggcattg
     4381 tttatatttc ccatcgttta aaagagattt ttgaaatttg tgatgatgtg acggtgctaa
     4441 gagacgggca atttatcggc gagcgagtaa ttgccgatat tgatgaagat cagttaattg
     4501 aaatgatggt tggtcgccgt ttggaagagc aatatccgca tttacaccaa gagcgtggcg
     4561 agttattact tgaagtgaag aatttaagtg gaagtggggt gaataatgtg tcattccaat
     4621 tacacaaagg tgaaattgtg ggtatttccg gcttaatggg ggcagggcgt acagaattaa
     4681 tgaaggtgct ttatggtgca ttgccgaaaa cagccggctc gatagagtta aatggaaaag
     4741 cgatttccaa ccattgtccg caagatgggt tgaataacgg cattgtgtat atttcagaag
     4801 atcgtaaagg cgatggtctg attttgggaa tgtcggtaaa agaaaatatg tctttgacct
     4861 ttctcgatca tttctccaaa gccggcagta ttcgccacga tgcagaaaaa ttggcggtcg
     4921 atgattttat tgagatgttt aacattaaaa ccccaagtcg ggagcaacaa atcgggctgc
     4981 tttccggtgg taatcagcaa aaagtggcga tcgctaaagg gttgatgacc cgcccgaatg
     5041 tgctgatttt agacgagccg acacggggtg ttgatgtggg ggcgaaaaaa gagatctatc
     5101 aattaattaa tgaatttaaa aaagatgggc ttagtatttt gatggtttcg tccgatatgc
     5161 cggaagtgtt gggaatgagc gaccgcattt tagtgatgca cgaagggcaa atcagtgcag
     5221 aattttcacg agcagaagcg acacaagaaa agctactggc agcagctatt ggcaaaagta
     5281 caggattaaa ttaaggtgtg tttatggcaa ctcaaacaaa gcagtttcaa cttggcagat
     5341 ttttaatcga gcaacgttct tttgtagcgt tatttatttt aattgtcagt gtttcaatga
     5401 ttaaccctga ttttttcagc gtggataata ttttaaatat tctacgccaa acctcggtga
     5461 atgcgattat tgcggtcgga atgacctttg tgattttaat tgcaggcatt gatttatcgg
     5521 tcggttcggt tttagcctta accggtgcgg ttgcagcgac cttagtcggt tctgaattgc
     5581 cgattttgtt ggtgattccg ctggttttat tattagggac ggcatttggt ggtattagtg
     5641 gggcgattgt tgctaaaggt aaagtgcagg catttattgc gactttggtc acaatgacct
     5701 tattgcgtgg gattacgatg gtttataccg atggcagacc gattagcacc ggcttttctg
     5761 accaagcaga cgcctttgcg tttattggga caggctattt atttggtatt cctgtgccga
     5821 tttggattat ggcggtagtg tttgggttag cttggtatat tttgaaacac accccgattg
     5881 ggcgttacat ttacgcttta ggtggcaacg aatcggcaac ccagctttca ggcatcaatg
     5941 tgaataaaat taagatcttc gtatttgccg tgagtggttt tttatctgct cttgccggct
     6001 taattgtgac ttcacgtctc tcttctgctc agccaacggc aggtgtgtcc tatgagttag
     6061 acgcaattgc agcggtagtt gttggtggaa ccagtctaat gggcggtaaa gggcgtgtaa
     6121 tgggaacgct tgtcggggca ttaattatcg gtttcttaaa caatgccctt aatttattgg
     6181 atatttcttc atattatcag atgattgcta aagcgttggt tattttagca gctgtattag
     6241 ctgataatta tctaggcact aaaaaagttt aatctatctt taaaggagat ttctatgaaa
     6301 aaattaacta cgattgcatc agcggttatg ttaagtttat cggttgcagc cactgcttcg
     6361 gcaaaagaga ctatcgctct tgcagtatcg actttagata acccattctt tgtgagcctt
     6421 aaagacggtg cacagaaaaa agcagatgaa ttaggctaca aattagtggt gcttgattcc
     6481 caaaatgacc cggctaaaga gctttccaat gtggaagact taacggtgcg tggggcaaaa
     6541 gtgttattga ttaacccaac cgattcggaa gcggtcggaa atgcggtggc tattgcaaat
     6601 cgcaacaaaa ttccggttat tacacttgac cgtggtgcag caaaaggcga agtggttagc
     6661 catatcgctt ccgataacgt tgccggcggc aaaatggcgg gagatttcat tgctcagaaa
     6721 ttaggctcag gggcgaaagt gattcaatta gaaggcattg ccggcacttc cgccgctcgt
     6781 gaacgtggtg aaggctttaa acaagcgatt gaagcacata aattcaatgt attagccagt
     6841 caaccggctg attttgaccg cactaaaggc ttaaatgtga ctgaaaactt gctctcaagc
     6901 aaaggggacg tacaagctat ttttgctcaa aatgatgaaa tggcactagg agctttgcgt
     6961 gcagtgagtt cagcgaagaa agaggttgta attgtgggct ttgacggcac agatgatggc
     7021 gtaaaagccg ttgaaagcgg taaacttgcc gcaacgattg ctcaacaacc tgaactgatt
     7081 ggctcattag gcgtagaaac tgccgataaa gtgctaaaag gcgaaaaagt ggaagcaaaa
     7141 attccggttg atttaaaggt tatttcaaaa taattatact tgaagatagc gttcgtctct
     7201 gacgaacgct ttgaattaat aaaatagcac tcgtcagaga ctagtgctat cagaatccta
     7261 gttttgttgt actaggtaat ttgagggctt aatatgaaaa aactcaccgt tctcggtagc
     7321 attaacgctg atcacgtgat ttccgttccc tattttgcca aaccggggga aacgctcacc
     7381 ggacatagct gccacattgc ttatggcggt aaaggggcaa atcaggcggt ggcggcagca
     7441 cgtttgggag ctaaggtttc tttcatcggt tgtattggca gtgatgacat cggcaaagca
     7501 atgaaatcag cctttgagca agatggcatt gacactcgct cgattaaaac gattccgaat
     7561 gaaatgaccg gcattgcaat gattcaagtg gcggaatcgg gcgagaacag tattgtgatt
     7621 tcagccggag cgaacggtca tttggatgaa acggttgtgg caaagtgtgc agccgaaatc
     7681 acacaggcgg attatttgct aatgcagcta gaaacaccgt tgccggcgat tgtgcaagca
     7741 gcaaaattgg ctcagcaaaa cggcacgaaa gtaatattaa accctgcgcc tgctcgagcg
     7801 ttacccgatg aactgttaaa cttactggat atgattaccc caaatgaaac ggaagcggaa
     7861 atattaaccg gtgtgaaagt tgttgatgaa gccagtgcga ttcaagccgc acaagtgttt
     7921 caccaaaagg gcatcgaaaa agtgttgatt acacttggct caaaaggggt atttgtaagc
     7981 gaaaacggtg taggcaaaat cgttgcaggc ttccgtgttc aagcggtgga taccactgcc
     8041 gcaggggata cttttaacgg agcattagtg actgcaatgt tagaagataa accgctggat
     8101 caagccattc gttttgctca cgcagcggca gcgattagtg tgactcgcaa aggggctcaa
     8161 ccttctattc cgacccgtca ggaaacactg gattttctaa gccaacaagc ggtgagtttt
     8221 taggaaaatt ttgcaaatgg caacaatgaa ggatattgca cggcttgcac aggtttccac
     8281 ctcgaccgtt tctcacgtta tcaacaatag ccgatttgtc agcgaggaaa ttcgggaaag
     8341 agtgcagagg gtggtgaaag aactgaatta cacgccttct gcattggctc gtagcctcaa
     8401 agtgaatgaa actaaaacca tcggaatgct ggtaacggca accggcaatc cgttttttgc
     8461 ggaggtgatg gcgggggtgg agcaatattg ccagcaacat caatataacc tgattattgc
     8521 cactaccaac ggtgacaccg agcgtttgca acaccatttg caaatgctga tgcaacgaaa
     8581 agtagatggc ttgttactga tgtgtggcga taccagattg aatgccgatg agagcttgaa
     8641 tattccgctg ccattggtgg tgttagactg gtggtttacc gagctcaatg cagataaaat
     8701 ctttgaaaat tcaatatccg gcggctatct cgcaacaaaa accttaattg aagcagggca
     8761 ttctaagatt gcgattatta ctggaaacct aaaaaaatca ttggcaaaaa atcgtttaga
     8821 aggctacaaa aaagcacttg ccgaggcgaa tattccactc aatccaaact gggtgttcga
     8881 aagccatttt gattttgccg gcggggttaa tggaatgaat gctttgcttg cccaaagcga
     8941 aaagccgaca gcggtatttg cctgtagcga caccattgct ctaggggctt accaagcaat
     9001 ctggcaacaa ggtttacgaa taccgcaaga tatttcgatt atcggctatg acgatattga
     9061 actggcaaaa tacctttccc caccgctgag taccattagc cagcccaaag cggaactcgg
     9121 gcggcttgcg gtggaaactt tgctaaaacg aattcgtttt aaatccactg attttcaaac
     9181 cataatgctt gaacctgaat tagttttaag gcattcaatc caaacaataa acaaaaacgc
     9241 tgaataatgt gaagttattc agcgtttttt atccgatttc aattagcctt tttgctgttc
     9301 caaataaagc acggtggcgg caactcggga atgcacgttt aatttacgca gtaaattgcg
     9361 gatatgcact tttacggttt cttcggaaat aaagagctga gccgcaattt gtttgttcga
     9421 cataccggtt gcgagccatt gtaaaacatc cagttcacga tctgttaagc aacttaaagg
     9481 atcattttct ggctggcggt tgagtaaatg ttggcgaacg gtttcgctta ataccacttc
     9541 accggcagcc gctttacgga tattatctag tagttccggg gtgtcggtat cttttaataa
     9601 atagccgtcc acaccagcat ccattaatgc aaatacatcg gattgctcgt ctgacactgt
     9661 taaaacaatg attctggcat ctataccttg agcgcgcaat gagcgtaaag tatctagccc
     9721 tgaaagccct ttcatattta aatccagaat aatcacatcc ggctcaagtc gtaatgcaag
     9781 tttgataccg tcggtgccgt tgcttgcttc acccactaca ctaaattggt gatctaattc
     9841 caatagttga cgcattccct gacgcattaa tgggtggtcg tcaatgagta aaatttgagt
     9901 tggagctgtc atttatttct cctttccttt ctcttaatcg gttaattttg caattattaa
     9961 ttcaaaatag caagtttagg gatttactaa atgccgcttt tttgatctta gctaattttt
    10021 aactaaaaaa gaaattgcaa aactttgcaa gaaaaagacc gcttgttcgg ctataattac
    10081 tgccaatttt tatttcaagt gtgaaaagaa tgacaaatca aacaacctat tttgccacgg
    10141 cagcccgtgg ttttgaggaa atactaaaaa ccgaactgga gcagatttgt gcggcagaat
    10201 gcaaagtcgc tcaaggcggt gtgcatttta ccaccacgca aaaaggggct tatcaggcat
    10261 tattacacag ccgtttagcc tctcggatct tactgccgct catcagcacc aaaattttta
    10321 gtgatagcga tttatatgct tccatcgtgt catttaactg ggcggattta ttcgatcccc
    10381 gtgatacctt ttacattgat ttcagcggca ctaaccgaga aatccgcaac acccaatttg
    10441 gggcaatgcg agtgaaagac ggcattgtgg attactttga acgcaaaggt tttgcccgcc
    10501 caacagtaga taaagataat ccggatgttc gcattcatgt ttatttggat cgtgaaaatg
    10561 ttgtggtttc gcttgattta agtggagatg ccttacatat tcggggctat cgggaagaaa
    10621 ccggtaaagc tccgttgcgt gaaacattgg cggcagccat tattttgcgt tccggctgga
    10681 aagtcggcac gccattggtt gatccgatgt gtggttcagg cacgttactg attgaagcag
    10741 cacaaatggc agcgaatatt gcccctcaac ttcaccgtaa acattggggc tttaatgcgt
    10801 ggaaagggca tcaacaagcg atgtggaaag ccgtgttaga tgaggcattc cgaaatgttg
    10861 aattgggtgc ggtaggggag aattgcaatt cgtccctaca aaaaatgttc ttcggttttg
    10921 atctcgatca tcgtgtgctg actaaagcaa agcaaaatgc gaaaaatgca ggggtagatc
    10981 atctcattca atggcaacaa ggtgatgtgg ctgcgttaaa aaatccgatt ccggaaaaaa
    11041 tcggcacggt ggtgtgtaac cccccttatg gtgaacgttt aggcacaacg ccggcgttaa
    11101 ttgctcttta ttcggtgttc gggcaacgcc taaaacagca atttgccggt tggaatgcct
    11161 caatttttag cggcgagcct gaattgttaa attgcctgcg tttacgttca gcccgtcaat
    11221 tcaaagcgaa aaacggtccg ttggattgtg tgcagaaaaa ttactccatc agcgagcata
    11281 acaagcggtc agatttagat gaaaatttac aatttgaaca gaatgctcaa gtcgctccgg
    11341 attttgcaaa tcgcttaacc aaaaatatca aaaagatcga aaaatgggcg aaacaacaag
    11401 gcattgatgc ctaccgttta tacgatgccg atctgcctga atataattta gcggtggaca
    11461 gatatggcga ccacattgtt gtgcaggaat accaagcccc gaaaaatatt gatgagcaaa
    11521 aagcccgcca acgcttgtta gatgcagtct ctgctacgct ttatgtgaca ggtgttgaaa
    11581 ctaataaatt agtgttgaaa gttcgccaaa aacaaaaagg cacgaatcag tacgaaaaac
    11641 ttgccaataa aggcgagtat ttctatgtga acgagtatgg ggcgaaactg tgggtgaatt
    11701 taaccgatta cttggatacg ggtttgttcc tagaccatcg cttaacccgt aaaatggtgg
    11761 gcgaaatggc aaaaggcaaa acattcctga atctgtttgc ctacacgggt tcagctacgg
    11821 ttcacgctgc tcttaatggg gcaaaatcga ccactacagt ggatatgtcg aacacctatt
    11881 tgaactgggc ggagcaaaat ttagagctga ataatttatc acttcgccat catcgtttgt
    11941 tccaagccga ttgcttgcaa tggctggcag aatgccgtga gcgatttgag ctgatttttg
    12001 tcgatccgcc gactttctcc aactcaaaac gaatggaaaa cagttgggac gtgcaacgag
    12061 accatattga gttaatgaaa cagcttaaac gtattttaac cgctgatggt accattgtgt
    12121 tctcgaacaa taagcgtggc tttaaaatgg attttgacgg tttggcagca ttaggcttaa
    12181 cggcggaaaa tatttcgcat aaaaccttac cgttggattt tgaacgcaat ccgcaaatcc
    12241 ataattgttg gattgtgaag aatgtataga taataccctc gacccttgtg ggagagggat
    12301 agatttttct tcgttcagaa gaaaaatcag ggagagggta agaagttact gatgaaagag
    12361 ttatttcccc ctctctctga aaaatatttc tgaacgaaat atttttcggt ctctctccca
    12421 caaggggaga gagtaaggct aaaaaatgtt agatattgtt ttatacgaac ctgaaattcc
    12481 gcaaaacagc ggaaatatta tccgcctttg tgccaactgt ggctttcgtt tgcatatgat
    12541 tgaaccgttc ggctttggtt gggacgataa aaagctccgc cgttcggggc tggattatca
    12601 cgaatttgtg gatattcaaa aatataaaaa ttttgaagat ttccttgagc gtgctaagcc
    12661 taaacgcttg ttcgccttaa ccaccagagg ggagcagaac cacagtgatg tgaaatatga
    12721 acttggcgat tttttaatgt tcgggccgga aagcagaggc attccaaagc cattattagc
    12781 ggaaatgccg atgtcgcaaa aaatccgcat tccgatgtgc aaagacagcc gcagtatgaa
    12841 tttatctaac tcggtggcgg tggttgtgta tgaggcgtgg cgacaattcg gctaccaaaa
    12901 tgcggtagcg gcacaatcta tttaacttgc aagcggtgtg attttggcta ttttttgcaa
    12961 gtttgtggtc aaggttgggg gaggctcgac cttgactaat taagaacatc tattgtgtct
    13021 attcttcttc atttttagct ggaggtaaaa aattctctcc ggtaacaact gatttacctg
    13081 ttttattctc caattcctga cgggcatttt tggcaatatt gccaccttgt tttcctgctt
    13141 gggcattttc atcaacccca actgcctgca tactttctgc aatttgccga ctggaaagtt
    13201 cagcaagtgc tgtaaataac aattcagctt cgctcatatg atctcgcaga ttttggcttt
    13261 ttaaaccttt aagcgattta tgcttttgta cagatagtcc gctccattct tgatgaatca
    13321 cattggttaa aaatgcaaaa tcttgtggct gttcaacccc tgacgatttc caataatcgg
    13381 tcagtttatt tcgggtttct tgtcccatca ttctttgctg aatccatttt tcacttcgtc
    13441 ccatcttctg ccaattctcc cttgctcgat ctagactttg acttggatcg tttatttcct
    13501 gtaagcgttc ataacctact tttgctaacc attgtttgaa gggctctgct ttcggactag
    13561 ggatagattg aataagtcgg aaaagttgtt cggtatctgc tacatcagtc aaacgcattt
    13621 ttccgtcagc agagggcatt ttcaacccgt gacaatttgt cacggtttca ttgccttctg
    13681 cctttagcct ctgttttagc ttacgccaat aagcagaggg atctaggctt tcggttaaaa
    13741 tagcaatcac atcaataata gaaaaatacc atttatcttt ttcttcatcg taatgactgc
    13801 gtacagattt ctgttcaaaa ag
//
LOCUS       DANLRK010000059        13785 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.059, whole genome shotgun sequence.
ACCESSION   DANLRK010000059 DANLRK010000000
VERSION     DANLRK010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 13785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13785
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.059"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            76..255
                     /locus_tag="PXA33_001224"
     CDS             76..255
                     /locus_tag="PXA33_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5120559.1"
                     /translation="MFKEKGYDEFLAEKIRLGLEDMQSGNGLSLDESKARTKQLIERK
                     ARELANFEQENIIYG"
     gene            248..535
                     /locus_tag="PXA33_001225"
     CDS             248..535
                     /locus_tag="PXA33_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL5120560.1"
                     /translation="MAKTLRISSRAQNDIEEILASVIEYTGFESSGIRLQEDIYQKFE
                     TIAYMPSAAGRLREDGTREAFTRRYRIVYTELENEVWIITIIHSSRLYPRI"
     gene            complement(661..2763)
                     /locus_tag="PXA33_001226"
     CDS             complement(661..2763)
                     /locus_tag="PXA33_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HDL5120561.1"
                     /translation="MKSFKLLPVSAAVLSVLAANAVAQTASTDAVVLDTVTVTDNQGL
                     KVQSNVVTTKKKDESTQTDLRGLLKDEPAISLGGGNGTSQYLYIRGMGQNSIDVKVDN
                     TYSDSQILYHQGRHMLDPALVKIVSVQKGAGSASAGIGQTNGAVIAKTVDALDLLKNS
                     DKNFGAKLGTGFSTNHAHNFNATLYGKGEIFDALVSGNLVRDRDYKGGKDYVNQFGTN
                     RVPYSALDKTSFLAKLGATLGDHRFVLSHSSERNEGQRLVREEFDVAPNGTKVSPYNR
                     LTVERQAPANRRITVQRTNLEWTGKNLGFAQEATANVYQLVQGRWSENETGNGYAGGV
                     DYATKTKIVTHGANVNFDSALHENVLLKYGVNYRHQEAKPHTKFNPLVKNQEKTDTGV
                     YVEAITAPVDKVTLTTGVRYDHFKFKAMDGKERSNGAFSPSVGLIYEPIEHLSLSASH
                     NYATRSPRLYDALTAHGNRKIVSIGSNTKAEKARNTEIGFNYNNGTFSFDGSYFWQDI
                     KDALGGGRDSHNPNNELIMNLGKIKNRGYELNAGYHNGGFTARIGVAHSKPRFYSPTI
                     WEVNRQTGKREEKPLLSTNPEYASAIGRTWTASLSYRFEQPNLEVGVHHRIVEKVKPE
                     DNFFVTGGTLQTNTGTGKDGYNVTDITLNWKPLKDDSVNVNFAVDNVANKKYNAHGQR
                     GQLSARGREFRAGVNYTF"
     gene            3046..3792
                     /locus_tag="PXA33_001227"
     CDS             3046..3792
                     /locus_tag="PXA33_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL5120562.1"
                     /translation="MQLRHKQIMEYIQQFDEANVQELAEHCGVSIETIRRDLNKLDKD
                     GLLHRTHGGAVSHKKRDIGRSFTTRQRLNSEAKRSIAENVLEYLQPESVIALDASSTS
                     WNVAQRIPNIPCTVVSSSMRIIYSLSHKPHIKTIATGGIYSEKYDAFYGPLSEQLLTR
                     LKIDLAILSCTGIADGAIWESNEINISLKRKLIANSKQVFLLADHSKFERKDLFRIED
                     LSCIDKLFINQPPPDFLKNYCLAHQIEIII"
     gene            3988..5751
                     /gene="fucI"
                     /locus_tag="PXA33_001228"
     CDS             3988..5751
                     /gene="fucI"
                     /locus_tag="PXA33_001228"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005619331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HDL5120563.1"
                     /translation="MTTLKSTPVKIGIRPTIDGRRLGVRESLEDQTMNMAKAVAQLIQ
                     SEVRHPNGDFVECVIADSTIGGVAEAAACAEKFKRENVGAVITVTPCWCYGSETIDMD
                     PHMPKAIWGFNGTERPGAVYLAAALAGHSQLGLPAFSIYGTEVQEADDQSIPDDVREK
                     LLHFARAGLAVATLRGKSYLSIGSVSMGIAGSIVNQQFFQEYLGMRNEYVDMTEIKRR
                     LDRHIYDAEEFKLAMSWVKTYCKEGIDVNAPENQRSPEEREKLWEDVVKMTIIARDLM
                     VGNPRLAELGYSEEALGHNAIAAGFQGQRQWTDHLPNGDFMEATLNSTYDWNGVRAPY
                     IMATENDSLNGVNMLFGNLLTGQAQIFADVRTYWSEDSVERVTGWRPESGFIHLINSG
                     SAALDGTGQHTDRAGKPTIKPAWEVTEEDGKRCLENTCWCPAVHEYFRGGLSSQFLTK
                     GGMPFTMHRINLIKGIGPVLQIAEGWSIDLPENVHDILNKRANETWPTTWFVPRLTGK
                     GAFTDVYSVMANWGANHCVATFGHVGADLITLASMLRIPVCVHNVEDKDVFRPSAWNG
                     FGQDKEGQDYRACANFGPLYK"
     gene            5945..7378
                     /gene="fucK"
                     /locus_tag="PXA33_001229"
     CDS             5945..7378
                     /gene="fucK"
                     /locus_tag="PXA33_001229"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HDL5120564.1"
                     /translation="MPISLIFDCGATNLRTIAINQAGEIVAAHHLPNNTQPDVESPEF
                     HIWDIEEIWSKLMTCAKQTLSQLSEQQRQEIVGISVTTFGVDGALFDKNGKQLYPIIS
                     WKCPRTLPIMENLANYLDVEALYQRNGVGHYSFNTLFKLLWLKENKPEIYQQAESFLF
                     ISSILTYLLTGVQTTDRTMAGTSMMTNIENDSWDKTVLDLLGLTENHFPPMKSAGEVV
                     GTLKGEISQELGLSGQIPVISCGHDTQFAIFGSGAGYNQPVLSSGTWEILMVRTPKAK
                     PQWQFVQNGLTIEFDSQSGYFNPGVQWVASGVMEWFGKRFFSDIANTEHYYRTMIEEA
                     AEVPAGSNGVKLVGNFDGTTAKTGSIIGLSMHITRGEIYRAGLEYMAHRLKEGLDVLQ
                     EVGHFNAESLICVGGGSKNALWNQIRADVLNRPIDVVDFPESTVLGAAMFTFTGAGVF
                     ENPNAAQQAMKPNVKRVEPSSNSQFYK"
     gene            7453..7884
                     /gene="fucU"
                     /locus_tag="PXA33_001230"
     CDS             7453..7884
                     /gene="fucU"
                     /locus_tag="PXA33_001230"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012341412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HDL5120565.1"
                     /translation="MLKGIHPAISPDLLKVLAEMGHGDELVLSDAHFPAHSIHQNVIR
                     ADGLSVATLLEGISALFEFDQYVEAPLAMMQAVKGDTLDPSVEERYLNAIKKVNGTAP
                     KIERVERFAFYDRAKNAYAVVITGELAKYGNIIIKKGVTPV"
     gene            7944..8597
                     /locus_tag="PXA33_001231"
     CDS             7944..8597
                     /locus_tag="PXA33_001231"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HDL5120566.1"
                     /translation="MDRKILARQIIDTCLEMTRIGLNQGTAGNVSVRYKDGMLITPTG
                     TPYEQMTEDSIVFVDGNGKHEEGKLPSSEWQFHLAVYEARPELNAVVHNHAKNCAAVS
                     ILGEPIPAIHYMIACTGTNHIPCVPYAPFGTHQLADYVREGIKHSKAILLAHHGLITA
                     DKTLEKALGIAHEVEVIAEWYLKLLATGKPIPTLNKEQMDIVLGKFKSYGSWVESEQ"
     gene            8659..10188
                     /locus_tag="PXA33_001232"
     CDS             8659..10188
                     /locus_tag="PXA33_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDL5120567.1"
                     /translation="MQNEVILTMKNISKSFAGVNALKGVELTLRKGEVHALMGENGAG
                     KSTLMKCLLGVYQADEGEIIYKGQPVRFESVLEAQKAGISMIFQELNLIPHLTVAENI
                     FFAREPLKHGLVDKDKMVEESAKLLNFFEIDVDPNDEVRMLSVAKQQMVEIAKALSFD
                     VEVLIMDEPTSALTEKEIDKLFELVDRLRAKGVSIVYISHRMEELKRICNHITIFRDG
                     TYVSNHKFNEITMDEIITKMVGRSLDNHFPPKTAVVTNDIILSIMNAERKGVFEPLNF
                     DLRKGEILGITGLVGAKRTELARAIFGADPLDSGEIFVHENKVSIKDPSDAIKAGIAY
                     LSEDRKLNGVAVRMSIRENITMASMDKVANQIGVISYDEEEKASKTFIDKMEIKTPTI
                     EQKVQNLSGGNQQKVVIGKWLFREAKVMIFDEPTRGIDVGAKYAIYQLLDELAANGVG
                     VIVISSELPEVLGVSDRIIVMREGRMTGMLETKKTNQEEIMHYATGVKNMFAREYGVE
                     K"
     gene            10185..11183
                     /locus_tag="PXA33_001233"
     CDS             10185..11183
                     /locus_tag="PXA33_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL5120568.1"
                     /translation="MNEKQKEMLRKMAALAGLVLLIIFFSVTNEYFFTSNNIMTVGLQ
                     TSTIALIGIGATCVILTGGIDLSTGSVVALSGVAAAMIVNAGVPVPLGMILGILVGGI
                     CGLINGILVTRMKLPPFIATLGMMMVARGLALYVTNAAPVSGMPESFANLGNGALFKI
                     VEEGANGLPKVVFAGIPYPVIIMIFITVLFTFALSKLKVGRYLYAIGSNEEAARLSGI
                     KTNIVKIYAYVASGLLSGLTGVILASRLVTAQPNGGVSYELDAIASAVVGGTSLMGGV
                     GTIPGTLIGSFIIGVLRNGLNMNGVSSFVQMIVIGLVIIVAVSLDQLRQSKKAGKK"
     gene            11207..12154
                     /locus_tag="PXA33_001234"
     CDS             11207..12154
                     /locus_tag="PXA33_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL5120569.1"
                     /translation="MKKSALFTATLFGLSTLFAGSAMAKSDEIAVIVKSANSTFWQNV
                     RKGAETAGKDIGGQYKVTFQGPEAETAIDAQVNMVDNAVNRGVAGIVLAASDPVALVP
                     AVKKAYESGIPVVLIDSGINSDGKYYQSFLATDNRAAGKLAAEKLLAKVKGGKVAVMS
                     FTQGAASAIERTGGFIDEIKAKADYKLVGPYYSDSDMTKALNQTEDVLGSNPDLAAIF
                     GANEPTAVGMARAVKQKGFAGKIVAVGFDGNSDLQNFVRDGTLDGIVVQSSYQMGYKG
                     VDTIGKLIKGEKVEKVIDTGVVYVTKENIDSEEAKGVLY"
     gene            12210..12461
                     /locus_tag="PXA33_001235"
     CDS             12210..12461
                     /locus_tag="PXA33_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIB"
                     /protein_id="HDL5120570.1"
                     /translation="MSNTLTDKALVVIAALGGRENIVDVYACITRLRVEVKNTDIVEW
                     DKIKSVGALGVVTSGSVIQSIFGSDAEAYRDEIKRILNI"
     gene            12590..13741
                     /gene="fucO"
                     /locus_tag="PXA33_001236"
     CDS             12590..13741
                     /gene="fucO"
                     /locus_tag="PXA33_001236"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HDL5120571.1"
                     /translation="MANRLILNETSYHGKGAIQNIVHEVTSRSFKKALVVTDKDLIKF
                     NVAQKVTALLDAAGLPYEIFDEVKANPAVDVIKAGVAKFKASSADYLIAIGGGSPIDS
                     AKAIGIIINNPEFSDVLSLEGVAPTHQKCVPIIAIPTTAGTAAEVTINYVITDEAKKR
                     KFVCVDVHDVPAVALVDPDMMESMPKSLAAATGMDALTHAIEGYITKAAWELTDALHL
                     KAIELIGRSLRGAVEKNPKDVEAMALGQYVAGMGFSNVGLGAVHSMAHPLSAYYDTPH
                     GIANAVLLPYVMEFNKNYTGEKYREIARAMGVKGVDEMSQEEYRNAAIAAVQQLSQDV
                     GIPPKLHQIGVKEADLAALSVDAFNDVCTGGNPRDCTAEELLEVYRIAY"
BASE COUNT         4132 a         2834 c         3008 g         3811 t
ORIGIN      
        1 taaaaataag ttaatttgac aactcaaaat taaaagcaga caatgctttt aattatatct
       61 taataggagc agattatgtt taaagagaaa ggctatgatg aattcctagc agaaaaaatt
      121 cgccttggct tagaagatat gcaatcaggt aatggtcttt cactcgatga atcaaaagca
      181 cgaaccaaac aactgattga acgtaaagca agagaactcg caaactttga acaagagaat
      241 atcatttatg gctaaaacat tacgaattag cagtagagct caaaatgata ttgaagaaat
      301 tttagcgagc gtaattgaat atactggttt tgaaagtagt ggaattcgct tacaagaaga
      361 tatttaccaa aaatttgaga ctatcgctta tatgccctct gcggctggac gtttaagaga
      421 agatggcaca agagaagcat tcactaggcg ttatcgtatt gtttataccg aattagaaaa
      481 tgaagtgtgg attattacca ttattcatag tagcagactt tatccacgca tttaaataac
      541 aaacgactaa aatagtcggt tttttatttg caaaaaatta ataaaatatc accgcttgta
      601 agctccataa aaaaaagccc gattgtaaca tcgggcgttt tagtctgaat ttaagtcaga
      661 ttagaatgtg tagttcacac ctgcacggaa ttcacgtcca cgagctgata attgaccacg
      721 ctgaccgtgt gcgttgtatt ttttattcgc cacgttatcc actgcgaagt ttacattcac
      781 gctatcatct ttaagtggtt tccagtttaa ggtgatatca gttacattgt agccgtcttt
      841 gcctgtaccg gtatttgttt gtaatgttcc gcccgtaacg aagaaattat cttcaggttt
      901 cactttctca acaatacggt ggtgtacgcc aacctctaaa tttggttgtt caaagcgata
      961 gcttaaagaa gctgtccaag tacgaccgat agctgaggcg tattctgggt tagtacttaa
     1021 taatggtttt tcttcccgct taccagtttg tctgtttact tcccaaatag ttggtgagta
     1081 gaaacgcggt ttgctgtgtg caacaccaat acgagcagta aaaccaccat tatggtaacc
     1141 tgcatttaac tcataaccac ggtttttaat tttacctagg ttcataatta actcattatt
     1201 aggattatgg ctatcacgac caccacctaa tgcatctttt atatcttgcc agaaatagct
     1261 accatcaaaa ctgaatgtac cattgttata gttgaaaccg atttcagtat tacgagcttt
     1321 ctccgcttta gtattgctac caatactaac gatttttcta tttccgtgag ccgttagtgc
     1381 atcgtataaa cgagggctac gagttgcata gttgtggctt gcacttaagc ttaaatgttc
     1441 aattggctca tagataagcc caacgcttgg gctgaatgca ccattactac gctctttacc
     1501 atccattgct ttgaatttga agtggtcgta acgtacaccg gtagttaaag ttactttatc
     1561 tactggagca gtaattgctt caacatacac gccggtatcg gttttctctt ggtttttcac
     1621 aagagggtta aatttagtgt gtggtttagc ttcttggtgg cgatagttca caccgtattt
     1681 taataataca ttttcgtgaa gggcagaatc aaagtttacg tttgcaccgt gggttactat
     1741 tttagttttg gttgcataat ctacaccacc agcataacca ttacctgttt cattctcaga
     1801 ccaacgacct tgtactaact gatacacgtt agctgttgct tcttgagcaa aacctaagtt
     1861 tttaccagtc cattctaagt tagttctttg aaccgtaatt ctacggttag ctggagcttg
     1921 gcgttctaca gttaagcggt tgtacggaga aacctttgtg ccgttaggtg ctacatcaaa
     1981 ttcttcacgc actaaacgct gaccttcatt tcgctcactt gaatggctta atacaaaacg
     2041 gtgatctccc aatgttgcac ctaatttagc taaaaagctg gttttatcta gtgcactata
     2101 cggaacacgg tttgtgccga attggtttac gtaatcttta ccgcctttat agtcgcgatc
     2161 tctcacaaga ttacctgaga ctaaagcatc aaagatttcg cctttaccat ataaggttgc
     2221 attgaaattg tgagcgtggt ttgtgctgaa gccagtacct aattttgcac cgaagttttt
     2281 atcgctgttt tttaataaat ctaatgcatc aacggtttta gcaattactg caccattagt
     2341 ttgaccaata ccggctgaag cactgcctgc acctttttgt acagaaacaa ttttcaccaa
     2401 tgccggatct aacatatgac gaccttggtg atacaaaatt tggctatctg agtaagtgtt
     2461 atctactttt acatcaattg agttttgacc cataccacga atatagagat attgtgatgt
     2521 accgttacca ccacctaagc taattgccgg ctcatctttt aataaaccac gcaaatcggt
     2581 ttgggtgctt tcatcttttt tcttagttgt taccacattg ctttgtactt ttaaaccttg
     2641 attatctgtt acggtaacag tatctaatac tactgcatct gttgaagcag tttgagccac
     2701 tgcattagca gcaagtacgc tcaataccgc agcagaaaca gggagcaatt tgaagctttt
     2761 cattttattt atattcctta ggtaaattat ttaagaggat tatctgtttg atattaaaaa
     2821 taattctcaa caccaaacgt tcgcaaattt tacagagata aaaatgattg tcaattagtc
     2881 tgaaatgaaa tttcacaaaa tatattacaa attttacaaa aagttcccta aatttaaccg
     2941 cttgcaacac aaaatgtgat cgccttctaa aaatcaaatt tgcttgctcg acagtaacgc
     3001 tacaagcggt taaatttgga gaaaaattta caaaaaggat agattatgca gctgcgacac
     3061 aaacagataa tggaatatat acaacagttt gatgaagcta acgttcaaga attggcggaa
     3121 cattgtgggg tttcgattga aactatccgc cgagatttaa ataagctaga taaagatgga
     3181 ttattacatc gcactcacgg tggagctgtg agccataaaa agcgagatat tggtcgctca
     3241 tttaccaccc gacaacggct gaacagtgag gcgaaacgca gtatcgcaga aaatgtgttg
     3301 gaatatttac aaccagaatc ggtgattgct ttagatgcca gctcaacctc ttggaatgtg
     3361 gcacagcgta ttccaaatat tccttgcacc gttgtcagca gttcaatgcg gattatctac
     3421 agcctttcac acaaaccgca cattaaaacg attgccaccg gtggtattta ttccgaaaaa
     3481 tacgatgcct tctatggtcc attatccgag cagttactga cccgtttaaa aattgatctt
     3541 gccattcttt cttgtacagg cattgccgat ggggcgattt gggaatcaaa tgaaattaat
     3601 atttccctta aacgtaaatt aattgcgaac agtaaacagg tttttttatt agccgatcac
     3661 agtaaatttg agcgtaaaga cttgtttcgt atcgaagatt tatcttgcat tgataaatta
     3721 tttatcaacc aacccccacc ggactttctc aaaaattatt gcttagcaca tcaaattgaa
     3781 atcattattt agtgatttct gcccgatttt attcagaaat aaaacaaatc gggcattctt
     3841 ttttcactca tttttaccaa aatcccccat tttttgtgat cgcactcaca aaatttaaac
     3901 taaattcgca tttttcttca taactcatat tgcgtatgca taataaattc aatcgcaaat
     3961 ttttttaacc aatcgggaga actatttatg acaacgttga aatctacccc tgtaaaaatt
     4021 ggtatccgcc caaccattga cggtcgccgt ttaggagtgc gtgaatcact tgaagatcaa
     4081 acaatgaata tggcaaaagc cgttgcacaa ttaattcaat ctgaagtccg ccacccaaat
     4141 ggtgatttcg ttgaatgtgt gattgctgat tccaccatcg gtggtgttgc cgaagctgcg
     4201 gcttgtgcag aaaaattcaa acgtgaaaat gtcggagcgg tgattaccgt aaccccttgc
     4261 tggtgctacg gttctgaaac cattgatatg gacccacata tgccgaaagc catttggggc
     4321 tttaacggaa cagaacgccc aggtgcagtt tatttagctg ctgctttagc aggtcatagc
     4381 caattaggtt taccggcatt ctccatttat ggcacagaag tacaagaagc tgacgatcaa
     4441 tctattccag acgatgtgcg tgaaaaatta ctccactttg cccgtgccgg cttagcagtg
     4501 gcaaccttac gaggaaaatc atatttatct atcggctctg tttcaatggg tattgccggt
     4561 tctatcgtaa accaacaatt cttccaagaa tatttgggta tgcgtaacga atatgtggat
     4621 atgaccgaaa ttaaacgccg tttagatcgc catatttacg atgccgaaga atttaaatta
     4681 gcaatgagct gggtgaaaac ctattgcaaa gaaggtattg atgtcaacgc accggaaaat
     4741 caacgcagcc cggaagagcg tgaaaaactc tgggaagacg tggtgaaaat gaccattatt
     4801 gcccgtgatt taatggtggg taatcctcgc ttagcagagc taggctatag tgaagaagcc
     4861 ttagggcata atgcgattgc tgccggtttc caagggcaac gccaatggac agaccatttg
     4921 ccaaacggcg attttatgga agctaccttg aattcgacct atgactggaa cggcgttcgc
     4981 gcaccataca ttatggcaac tgaaaacgac agcttaaatg gcgtgaatat gttgttcggt
     5041 aacttattaa ccggtcaggc acaaatcttt gccgatgtgc ggacatactg gagcgaagac
     5101 tcggtagaac gtgtcaccgg ctggcgtccg gaaagcggtt ttatccactt aatcaactcc
     5161 ggctctgcgg cgttagacgg aacaggtcag cataccgata gagcaggcaa accaactatt
     5221 aaaccggctt gggaagtgac tgaagaagac ggtaaacgtt gtttagaaaa cacctgttgg
     5281 tgtccggcag ttcacgaata tttccgtggt ggtttatctt cccaattctt aaccaaaggt
     5341 ggaatgccgt ttacgatgca ccgtatcaac ctgatcaaag gtatcgggcc ggttttacaa
     5401 attgccgaag gttggtctat cgacttgccg gaaaatgtac acgacatttt aaacaaacgc
     5461 gccaatgaaa cctggccgac cacttggttc gtacctcgct taaccggaaa aggcgcattt
     5521 accgatgtgt atagcgtaat ggcgaactgg ggtgcaaacc actgtgtggc aaccttcgga
     5581 cacgttgggg cagacttaat taccctcgcc tcaatgctcc gcattccggt ttgtgtgcac
     5641 aatgtggaag ataaagacgt attccgccca agtgcgtgga acggtttcgg tcaagacaaa
     5701 gaaggtcaag actaccgtgc ttgtgctaac ttcggaccat tatataaata atacacaatc
     5761 accctctccc cttgtgggag agggacagcc aagcaattcg ttcagaattg aatggcaggg
     5821 agagggctac aagcggtcgc ttttctctca aaatttacaa atccctctct ctgattaaat
     5881 tgctaaacgc aatttaatct atctctctaa aagggagata ttaggataag taaaggagat
     5941 aacaatgcca atttctctta ttttcgactg tggtgccacc aacctccgta cgattgcgat
     6001 taatcaagcg ggtgaaattg tggcagccca tcatctaccg aataacacac aaccagatgt
     6061 ggaaagccca gaatttcata tttgggatat tgaagagatc tggtcgaaat taatgacctg
     6121 tgccaaacag acgctttctc aactttcaga acaacaacgg caagagattg ttgggatttc
     6181 agtaaccacc tttggcgtgg acggtgcatt atttgacaaa aacggcaagc agctttatcc
     6241 gatcatttct tggaaatgcc cgagaaccct accgattatg gaaaaccttg ccaattatct
     6301 tgatgttgag gcgttatatc aacgtaatgg cgttggacat tatagtttca acaccttatt
     6361 caaattattg tggttaaaag agaacaaacc agaaatttat caacaagctg agagtttctt
     6421 attcatctca tctattttga cttaccttct tacaggcgtg caaacaaccg accgcacaat
     6481 ggcgggaacg tcaatgatga caaacatcga aaacgattcg tgggataaaa cagttttaga
     6541 tttattaggc ttaaccgaaa accattttcc tccaatgaaa agtgcaggag aagttgtggg
     6601 aacattaaaa ggagaaattt cccaagagtt aggtttaagc ggtcaaattc cagtaatttc
     6661 ttgcggacac gatactcaat ttgccatttt tggctcagga gctggctata accaacctgt
     6721 gttaagttca ggcacttggg aaattttaat ggttcgtacc ccaaaagcaa aaccacaatg
     6781 gcaattcgtt caaaacggct taaccattga gtttgatagc caatctggtt atttcaaccc
     6841 aggtgtgcag tgggtagctt caggtgtgat ggagtggttt ggcaaacgtt tcttctctga
     6901 tatagccaat actgaacatt actaccgcac aatgattgaa gaagcggcag aagtgcctgc
     6961 tggctcaaac ggtgtgaaat tagtcggcaa ttttgatggc acaacagcta aaacaggttc
     7021 aattatcggc ttatctatgc acatcactcg tggcgaaatt taccgtgcag gcttggaata
     7081 tatggctcat cgtctaaaag aagggttaga tgtattacaa gaagtcggtc attttaatgc
     7141 tgagagtttg atttgtgtgg gaggtggttc taaaaacgct ttatggaatc aaattcgagc
     7201 cgatgtctta aatagaccga ttgatgtagt ggatttccct gaaagtaccg tattaggcgc
     7261 ggcaatgttt acttttacag gtgctggtgt atttgaaaat ccaaatgcgg ctcaacaggc
     7321 aatgaagcca aatgtgaaac gggttgagcc atcgtcaaat agtcaatttt ataaataaag
     7381 tttctcccct ctctctgatt tgaggttcat tcagaacatc aaatcagaga gagggtaaaa
     7441 aaggagttca aaatgttaaa aggtattcac ccggcaattt ctccagattt attaaaagtc
     7501 ttagccgaaa tggggcacgg cgatgaatta gtgctttctg atgcacactt ccctgctcac
     7561 tctattcatc aaaacgtcat tcgtgcagat ggcttaagtg ttgccacctt attagaaggc
     7621 atttctgctc tgtttgaatt tgaccaatac gttgaagccc cacttgcaat gatgcaagca
     7681 gtaaaaggcg acacgctcga tccaagcgtt gaagagcgtt acctcaatgc aattaaaaaa
     7741 gtaaacggaa cagccccgaa aatagagcgt gtagaacgct tcgctttcta tgatagagcc
     7801 aaaaacgctt atgccgttgt cattacaggc gagttagcca aatacggcaa cattattatc
     7861 aaaaaaggcg taacaccggt ttaattacaa gcggttaaaa aagcagaatt ttttacaaat
     7921 ataaaattaa aaggagttca acaatggaca gaaaaatact ggcgagacaa atcattgata
     7981 cttgtcttga aatgacccga attgggttaa atcaaggtac agcaggcaat gtgagcgtac
     8041 gttataaaga cggtatgctc attacgccaa caggtacacc ttatgagcaa atgaccgaag
     8101 attctatcgt ctttgttgat ggcaacggca aacacgaaga gggaaaactc ccttcaagtg
     8161 agtggcagtt ccatttagcg gtttatgagg ctcgccctga attaaatgcg gtagttcaca
     8221 accacgctaa aaactgtgcc gcagtgtcta ttttaggtga acctatccct gccattcact
     8281 atatgatcgc ctgcaccggc actaatcaca ttccttgtgt accttatgcc ccgttcggca
     8341 ctcaccaatt agccgactat gtacgtgaag gcattaaaca cagcaaagca attttactcg
     8401 ctcaccacgg cttaatcacc gcagataaaa ccctcgaaaa agccctcggc atcgcccacg
     8461 aggtggaagt gatcgcagaa tggtatttga aactgctcgc tacaggaaaa cctatcccaa
     8521 cgctaaacaa agaacaaatg gatatcgtac tcggaaaatt taagtcctat ggttcttggg
     8581 tggaaagcga acaatagcct gccataaata acatacaagc ggtgatattt cttaaaattt
     8641 ttacaaacgg agtgcagtat gcaaaatgaa gtaatactca cgatgaaaaa catcagtaaa
     8701 tcctttgcgg gggtcaatgc gttaaaaggt gttgagctta cgctgcgtaa gggcgaagtt
     8761 cacgcattaa tgggcgaaaa cggtgcaggt aaatccacct tgatgaaatg cttgctgggc
     8821 gtatatcaag ccgatgaagg tgaaatcata tataaaggtc agcctgttcg ctttgagtcg
     8881 gtattagagg cacaaaaagc gggcattagt atgattttcc aagaactgaa cctcattccc
     8941 catttaacgg tggcagagaa tattttcttc gcccgtgagc cactcaaaca cggtttagtg
     9001 gataaagaca aaatggtgga agaatcggct aaattgctga atttctttga gattgatgtt
     9061 gatccaaacg atgaagtgag aatgttgtcg gtagccaaac aacagatggt agaaattgcc
     9121 aaagcccttt catttgatgt ggaagtactg attatggacg agccgacctc cgctcttacc
     9181 gaaaaagaga tcgataaatt atttgaattg gtcgatagac tgcgtgccaa aggggtgagc
     9241 attgtctata tttctcaccg aatggaagaa ttaaaacgta tctgtaacca tattacgatt
     9301 ttccgtgatg gcacctatgt atctaaccat aaattcaatg aaatcacgat ggatgagatc
     9361 attaccaaaa tggttgggcg ttcattggat aaccatttcc caccaaaaac cgctgtggtg
     9421 acaaacgaca ttatcctctc gattatgaat gctgaacgta agggagtgtt tgaaccattg
     9481 aactttgact tacgcaaagg agaaattctg ggcattaccg gcttggttgg agcaaaacgt
     9541 actgaactgg ctcgagccat cttcggagca gacccacttg atagcggtga gatttttgtg
     9601 cacgagaaca aagtcagcat taaagatccg agtgatgcaa ttaaagccgg tatagcctat
     9661 ttgtcggaag accgtaagct caatggtgtt gcagtaagaa tgtcgattcg tgagaatatc
     9721 acgatggcat ctatggataa agttgccaat caaataggtg tgatttctta cgatgaagaa
     9781 gaaaaagcct caaaaacttt catcgacaaa atggaaatca aaaccccaac gattgagcag
     9841 aaagttcaaa acttaagtgg tggtaaccaa caaaaagtgg taattggtaa atggttattc
     9901 cgtgaagcaa aagtgatgat ttttgatgag ccgactcggg gaattgatgt cggagcaaaa
     9961 tatgctatct atcaactgct cgatgagctt gctgccaatg gcgtgggtgt tatcgtgatc
    10021 tcttccgaat tgcctgaagt cttgggagtt tccgaccgca tcattgtaat gcgtgaaggt
    10081 agaatgacag gtatgctcga gactaaaaaa accaaccaag aagaaattat gcattatgca
    10141 actggtgtta aaaatatgtt tgctcgtgaa tatggggtgg aaaaatgaat gaaaaacaaa
    10201 aagaaatgtt aagaaaaatg gctgcattgg ctggcttagt actattaatc atctttttca
    10261 gcgtaaccaa tgaatacttc tttacctcca ataacattat gacagtaggg ctacaaacct
    10321 ctaccattgc gttaattggt attggggcaa cctgtgtgat cttaaccggt ggaattgatt
    10381 taagtaccgg ttcagtagtt gcactctccg gtgtggcagc tgcaatgatt gtaaatgccg
    10441 gtgtacccgt accactagga atgattttag ggattttggt cggcggtatt tgtggcttaa
    10501 tcaacggcat tttagtcacc agaatgaaat tgcctccgtt tattgctacc ctcggtatga
    10561 tgatggttgc ccgtggttta gcattgtatg ttaccaatgc cgcaccagtt tccggaatgc
    10621 ctgaatcctt tgccaatctc ggtaacggag cattatttaa aattgtggaa gaaggggcaa
    10681 acggcttgcc gaaagtggta tttgcaggta ttccttaccc tgtgattatt atgatcttta
    10741 ttaccgtact attcactttc gctctctcta aactgaaagt cggacgctat ctttacgcaa
    10801 ttggttctaa cgaagaagcc gcacgcttat ccggcattaa aaccaatatt gtcaaaatct
    10861 atgcctatgt tgccagtggc ttactctctg gtttaactgg cgttatcctt gcctcccgcc
    10921 tcgtcaccgc ccagccaaac ggtggggtct cttacgagct ggatgcgatt gccagtgctg
    10981 tggtgggcgg cacatcgctt atgggcggtg tgggtactat ccccggcaca ctcatcgggt
    11041 cattcattat cggggtactc cgtaatggtt tgaatatgaa tggcgtttct tcatttgttc
    11101 aaatgattgt catcgggctt gtgattatcg tggcggtctc tctcgaccag ctccgccaat
    11161 ccaaaaaagc cggtaaaaaa taatccactc ttaaaggaga attaaaatga aaaaatcagc
    11221 attatttact gcgaccctct ttggtttatc gaccttattt gcaggcagtg caatggcaaa
    11281 atctgatgaa atcgctgtga ttgtgaaatc tgccaactct accttctggc aaaacgtgcg
    11341 taaaggggct gaaactgccg gcaaagatat tggaggacaa tacaaagtca ccttccaagg
    11401 gccggaagcc gaaacagcga ttgacgccca agtgaatatg gtggacaacg ccgtaaaccg
    11461 tggcgtggca ggcatcgtac tggctgcctc agacccggta gcattagtgc cggcggtgaa
    11521 aaaagcttat gagagtggca ttccggtggt gcttatcgat tccggcatca acagtgacgg
    11581 caaatactac caatctttcc ttgccaccga caaccgtgca gcaggtaaat tagcagcgga
    11641 aaaactgctt gccaaagtca aaggcggcaa agtggcagtg atgagcttta cccaaggggc
    11701 agcatcagcg attgagcgta ccggtggttt tattgacgaa atcaaagcaa aagccgatta
    11761 caaactggtc ggcccgtact actccgattc ggatatgacc aaagcgttaa accaaaccga
    11821 agacgtatta ggttctaacc ctgatcttgc agcgatcttc ggggcgaacg aaccgactgc
    11881 ggtaggaatg gcacgagcgg tgaaacaaaa aggctttgcc ggcaaaattg tagcggtggg
    11941 ctttgatgga aactccgacc tacaaaactt cgtgcgtgac ggaacactag acggcatcgt
    12001 ggtgcaatct tcctaccaaa tgggctataa aggcgtggac accatcggca agctgattaa
    12061 aggcgagaaa gtcgaaaaag tgattgatac cggggtggtt tatgtcacga aagaaaacat
    12121 tgatagcgaa gaagcgaaag gtgttcttta ctaacattca agttaaacgg ggtggggttg
    12181 cccacccttt cagccgaaag gaagccaata tgtcaaatac ccttaccgat aaagccttag
    12241 tggtgattgc cgcacttggc ggtcgagaaa atattgtcga tgtttacgcc tgtattaccc
    12301 gcctacgtgt ggaggtgaaa aataccgaca ttgtggaatg ggataaaatt aaatctgtcg
    12361 gtgcgttagg cgttgtgacc tcaggctcag ttattcaatc tatttttggt agcgatgccg
    12421 aagcctaccg tgatgaaatc aaacgtattt tgaatattta gcctaagcac actcacaaca
    12481 aaagaatcgc aacaagcggt gaaattttta tcaaaatttg caaaaaaatg acgtaatttg
    12541 accgcttgca agttaatcga attgtttaac ttttaagaag gaaaatagta tggcaaatcg
    12601 tcttatcctc aatgaaacca gttaccacgg caaaggagca atccaaaata ttgttcacga
    12661 agtgaccagc cggagtttca aaaaagccct tgtggtaaca gacaaagacc tgatcaaatt
    12721 taacgttgcc caaaaagtca ccgccttgct tgatgccgca ggcttgcctt atgagatttt
    12781 cgatgaggtc aaagccaacc cggcagtcga tgtgatcaaa gcgggggtcg caaaattcaa
    12841 agcctccagt gccgattatt taattgcgat tggtggcggc tcaccgattg atagtgccaa
    12901 agcgattggt attatcatca ataaccctga gtttagcgat gtactttcat tggaaggtgt
    12961 cgccccaacc caccaaaaat gtgtgccgat tatcgccatt ccaactaccg caggtacagc
    13021 cgcagaagtc acgattaact atgtcattac cgatgaagcg aaaaaacgca aatttgtgtg
    13081 cgtggacgtt cacgatgtgc cggcagtggc gttagtcgat ccagatatga tggaatctat
    13141 gccgaaaagc ttggctgccg caaccggtat ggacgctctc acccacgcca ttgaaggcta
    13201 cattaccaaa gcggcgtggg aattaaccga tgctctacac cttaaagcga ttgagctgat
    13261 cggccgttca ttgcgtggtg cagtggagaa aaatccgaaa gatgtcgagg caatggcatt
    13321 gggacaatat gtggctggaa tgggcttctc caacgttggg cttggcgcgg ttcacagtat
    13381 ggcacacccg ctttctgcct attacgacac cccacacggc attgccaacg cagtattact
    13441 gccgtatgtg atggaattta ataaaaatta caccggcgaa aaataccgtg aaattgcccg
    13501 agcaatgggg gtaaaaggcg tagatgaaat gagccaagaa gagtaccgca acgccgccat
    13561 tgctgccgta caacagcttt cacaagatgt cggcattccg ccaaaactgc atcaaatcgg
    13621 cgttaaagaa gccgatttag cggcattatc ggtcgatgcc tttaatgatg tctgcaccgg
    13681 tggcaacccg agagattgta cggctgagga gctgttggag gtatatcgga tcgcatacta
    13741 agttctatct ttgttaaata gacaagcggt gattttttaa taaaa
//
LOCUS       DANLRK010000060        13691 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.060, whole genome shotgun sequence.
ACCESSION   DANLRK010000060 DANLRK010000000
VERSION     DANLRK010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 13691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13691
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.060"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            34..507
                     /gene="nrdG"
                     /locus_tag="PXA33_001237"
     CDS             34..507
                     /gene="nrdG"
                     /locus_tag="PXA33_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HDL5120572.1"
                     /translation="MENLRFSSEQIVWQEVPNETSLAFLITGCPLGCKGCHSADSWKA
                     CLGKELSSEYLSERLKRYQGLISCVLFMGGEWEAEKLLPLLQLAKKQGLNTCLYSGLE
                     QHEIAETLLHELTYLKTGRWIAERGGLESLTTNQRFTDLRNNQNLNHLFIRGASI"
     gene            504..2285
                     /gene="nrdD"
                     /locus_tag="PXA33_001238"
     CDS             504..2285
                     /gene="nrdD"
                     /locus_tag="PXA33_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005611280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HDL5120573.1"
                     /translation="MIRLQPTQLDGKLHFINQYIQAKNAADGSKMDANANVTQKNIAT
                     MEQELMKDFFVQINRAKVSEKIAKIFGKETACEYLRQIEAHEIYVHDETSLKPYCVSV
                     TMYPFLRDGLTKLGGESKAPKHLASFCGSFINFVFAVSSQFAGAVATVEFLTYFDYFA
                     RKDYGDNYLQTHRSDIENHLQQVVYSINQPAAARGYQSVFWNISVYDKFYFEAMFSRF
                     VFPDFEKPNWETTSKLQLFFMQWFNKERSKAILTFPVVTAAMLTEKGKCKDDEFANQM
                     AKELSQGNSFFIYQSDNPDSLASCCRLRNEISDHSFSYSLGAGGVATGSINVITINMN
                     RLVQDNRDLSEEVSKIHKYQYAYRKLMEEYLAAGMLPVYDAGFISLHKQFLTIGINGM
                     AEAAESQGLTVGYNPDYIDFVQSRLKIIFQANQAAGEKYGVKFNTEFVPAENLGVKNA
                     KWDKEDGYFVPRDCYNSYFYVVEDEEINALDKFLLHGRELIEWLDGGSALHLNLDEAL
                     TQTGYRSLLDTAAKTGCNYFCINVKITICNECSHIDKRTLHSCSKCGSDNIDYGTRVI
                     GYLKRVSAFSSARQKEHSLRFYHREAA"
     regulatory      2342..2438
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2495..3313
                     /gene="thiM"
                     /locus_tag="PXA33_001239"
     CDS             2495..3313
                     /gene="thiM"
                     /locus_tag="PXA33_001239"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HDL5120574.1"
                     /translation="MTTSLPYHSHYLQKIRQNRPLVHNITNIVAANFSANGLLALGAS
                     PIMAAAIEEMESVPALSNAVVINIGTLIGKEVEAMELAGKTANRLGIPLILDPVGVGA
                     TPFRRQTVETLVDQIHFTAIRGNAGELATLAGVEWQAKGVDAGEGSHNVVEIAQQIAK
                     RYQTLVAISGETDVVSSGSHTALLHNGSPMFPKITASGCLLSAVCGAFLAVADKEHYF
                     NALIEACAAYAVAGEVAAQALKPTQYGQFAVNLLDTLGALTPETVEQYVRVEYV"
     gene            3306..4619
                     /locus_tag="PXA33_001240"
     CDS             3306..4619
                     /locus_tag="PXA33_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL5120575.1"
                     /translation="MSKTHSNPTKVAVASMVGTAIEYFDNYIYTMAAVLVFNVQFFNS
                     DDPVSNQLMSLSVLALAFFTRPMGSILFGHFGDKYGRKQTLIASLLLMGLSTVAIGLL
                     PNYAAIGIWATVLLCLCRIGQGLGLGGEWGGAALVATEHAPKHKRAFFGIFPQMGAPI
                     GLLLANGAFYLVNLIYGEQTFLDWAWRIPFVASIVMIAIGLYIRIKISESPIFLKAEQ
                     QGKNRHTPLKTLFSQHLKPFMIGVLIATAGYVLFYILVAFTPIFVKSPVAVSQAGHAT
                     GLGLPVNLYTQYLLITSVIFGIAIFFSGIYSDKIGRRLLLLLATGATVVYGLTMPFFL
                     ENGTPTSIFIFLTIGMILIGLSFGPMAVILPELFPTEVRYTGASLAYTAAGILGASVF
                     TIIAVKINENFGLFGVGGYLSVASLLSFWALWQVHETKDLELEEI"
     regulatory      4755..4851
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4919..6448
                     /gene="thiE"
                     /locus_tag="PXA33_001241"
     CDS             4919..6448
                     /gene="thiE"
                     /locus_tag="PXA33_001241"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HDL5120576.1"
                     /translation="MSNYSNSPYQPNVIWTVAGSDSCAGAGLQTDLHTFHDFNLVGCS
                     VVTSVTAQHPHGVLCVTPVDDHTFRQQFEALLVQGYPNAIKIGLLCSQAQVEILCEYI
                     QKIRAESSHYCYVVYDPVAVASSGQALSDSILLQIVQQKLYPLVDLITPNGTELALLS
                     DTEIQTFGDVKTASHKLFAQGIKAVLAKGGHFEWLGETVRDYLLTANEIYAFSHARQQ
                     SVNTHGTGCTYASAVGAMLATGFDLADAVTVATAYLQKGLSEKQGRGQTALSSLCHTG
                     YPDDIAFFPQTELISAPLKLPKEPFAPTEYQLGLYPVVDSVKWLERLILVGVKTLQIR
                     IKNRPLAEIEQEIAHSVALAKQHNVRLFVNDYWQLAMKYQAYGVHLGQEDLATADLNA
                     IQQSGLRLGVSTHCYFEIMRALAVKPSYIAFGHVFPTQTKVMPSQPQGLTNLAKYVAL
                     VAPLNIPTVAIGGINEQSIENVMHTGVGSAAVVSAVTQAKDWQQAVKNLAKFANGGRN
                     E"
     gene            6441..7613
                     /gene="cytX"
                     /locus_tag="PXA33_001242"
     CDS             6441..7613
                     /gene="cytX"
                     /locus_tag="PXA33_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydroxymethylpyrimidine transporter
                     CytX"
                     /protein_id="HDL5120577.1"
                     /translation="MNRTLSTLNSGLIWAGAGISASAIVVGTWIAPLGWQQGLLAIVL
                     GHLLGGILFFAAGLIGAKTGKNAMQTVQISFGRGSVFFSLANVLQLVGWTAIMIYTGA
                     EISNALSLALWEYSAFSLWAIVLGLLIVLWLFTGSNQVGKFKLVTLIAMFLLTLWLSI
                     KVLNGQENFPVSGEMPFSTAVELATIMPLSWLPLVSDYTAKAKKPFAATLSATIGYLI
                     TSAWMYALGLGMVLFTGQTEIAPMLLMTGMSLIGIMVMILSTVTTTFLDAYSAGVSAG
                     NITAKFKETPTAILVTIIGTLLAISLPVTQYENFLLLIGSVFAPMIAVQIADFFVLKR
                     HQAQPNVDWVAAGLWLVGFVLYHLLSYHQVHLLLGLTIPVMLVIFLSTIFVRRFTK"
     gene            7652..7861
                     /locus_tag="PXA33_001243"
     CDS             7652..7861
                     /locus_tag="PXA33_001243"
                     /inference="COORDINATES: protein motif:HMM:NF015737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDL5120578.1"
                     /translation="MESLVLNNDWVVTPQAQLAYHTINGKADEERLNLLVARAGVRVA
                     KGFALNNGWNLQPYAEVNGIAEKGQ"
     gene            7869..8045
                     /locus_tag="PXA33_001244"
     CDS             7869..8045
                     /locus_tag="PXA33_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDL5120579.1"
                     /translation="MQVNQYQFGVAENKGRVQGSLGLSAGNGNHRLGLEASVASGKKL
                     KQPFNVLVNYRYQW"
     gene            complement(8086..9942)
                     /gene="sppA"
                     /locus_tag="PXA33_001245"
     CDS             complement(8086..9942)
                     /gene="sppA"
                     /locus_tag="PXA33_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HDL5120580.1"
                     /translation="MLTILKAFYRIFRCIRECVINLFFILFILLLVPVVAFISSSGST
                     QKPVFNQGALRLNLDGYLADNREEFTDFYRLVQSELGSSEPFKISTFDVVQAISHAKN
                     DPKITGLVLDLQKLQSADFSSLDFIGNEINNFKQSGKPVIAVGEDYSQKQYYLASFAD
                     EIYLNKVGAVDLKGLNYSNTYFKTLLDKIEAEPHIFRVGTYKSAVEPFMRDDMSEEAK
                     QNARGWLNGTWQQTVATLAQNRNIEPQALDLSPANYIEKYKQAKGDDAQLALNQKWVS
                     QLVSSQESRDKLIAQFGKNSEGSYNQIEFLDYMTELNDRFAEVNAPKIAVVNVEGAIA
                     GGESDEMNVGSETLVKLLQQARDDKNVEGLILRINSPGGSAVASELIRQEVEAFQQAG
                     KPVVASMGGMAASGGYWIAATSDKIIASPNTLTGSIGIFGLAVTFEKTAKNLGVSEDG
                     IATSALAQQAGLKSLPQEQAEVLQIGIENGYDRFLELVSRGRKMPKEVVDKVAQGQVW
                     LGTDALKHGLVDELGNFNVAYNALSELINQKREAENQPKVEQFGLQWFAEQDDSLFGT
                     LARDFKAQLQFSVANWLDLPLLKQPQQQLETLSRFNDPKQMYLYCLNCGKID"
     gene            10045..10608
                     /locus_tag="PXA33_001246"
     CDS             10045..10608
                     /locus_tag="PXA33_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDL5120581.1"
                     /translation="MQILDLLHHRRSNKKFGDKAPNAEQLEQILKAGLRVPDHGRLKP
                     YRFVVIEKSGMPQFGECLKSAVAEFDMGEERLKKAEKLANQAPMIIGVVATLDDSIAK
                     VPSWEQMITAGCATYAIQLAANAQGFDTVWITNKWINGSALRSAFGCQESDKIIALIM
                     LGSPAEGEQIALAPESESLEGFVRYLK"
     gene            complement(10679..13003)
                     /locus_tag="PXA33_001247"
     CDS             complement(10679..13003)
                     /locus_tag="PXA33_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HDL5120582.1"
                     /translation="MTDLNLKISQIIAGELNVGSHQILAAITLLGEGNTIPFIARYRK
                     EVTGGLDDTQLRHFETRLIYLREMDERRQTILKSIEEQGKLTDELKAKILSSESKTEL
                     EDLYLPYKPKRRTRGQIAIEAGLEPLADSLWNDPKQDPETLAANFVDAEKGVADTKAA
                     LDGARYILMERFSEDAELLAKLRQYLTAYATLESKVIDGKEEEGEKFRDYFAHSEPFN
                     SVPSHRALAMFRGRNEGVLSLSLNADPTAEEGTKSSHCEEIIREHLGVIFNNQPADKW
                     REQVIAWTWKIKAALHLETELMATLCEKAEEEAIDVFARNLSALLMAAPAGARNTMGL
                     DPGLRTGVKVAVVDNTGKLLDTATIYPHTGREAEAQLAIFSLIKQHNVELIAIGNGTA
                     SRETERFAKAVIKEIKENKPQTVVVSEAGASVYSASEFAANEFPNLDVSLRGAVSIAR
                     RLQDPLAELVKIEPKAIGVGQYQHDVNQSQLARKLDAVVEDCVNAVGVDLNTASAPLL
                     ARVAGMTKTLAQNIVAFRDENGRFTSRSELKKVPRLGPKAFEQCAGFMRIIGGKNALD
                     ASSVHPEAYPVVEKILQATTSTLAELMGNNSKIHSLNAKDFVDEQFGLPTVNDIFKEL
                     EKPGRDPRGEFKTAVFMDGVEEITDLKAGMILEGTVTNVANFGAFVDIGVHQDGLVHI
                     SMLSNSFVEDPHTVVKAGDVVKVKVLEVDAARKRIALTMRLDDKPTDKKEPTSGRNSA
                     EICKNTQKNDRLQSNNRNAFGNNAFADALKGWKK"
     gene            complement(13003..13557)
                     /gene="pilW"
                     /locus_tag="PXA33_001248"
     CDS             complement(13003..13557)
                     /gene="pilW"
                     /locus_tag="PXA33_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HDL5120583.1"
                     /translation="MFAKFFPKMTACILIFLLAACSSLPPSSSEVEFNRSEAVKARIN
                     LALAYLEQNDFPKAKQNIDKALAHDEKDYLPYSVLAYYYQQTGDAENAEKAYQTALKL
                     SQNRPDVLNNYGTFLCKQGQFQQAYQQFEQAVKSKAPYYHQADSLENMVLCAKKEPNL
                     QKIAETLTELEKLDKPRANLLKEN"
BASE COUNT         3687 a         3124 c         2975 g         3905 t
ORIGIN      
        1 aaaaacacaa tatattgtgt taatgaatta gtaatggaaa atctcagatt cagctcggaa
       61 caaattgttt ggcaagaagt gccgaatgag acctcacttg cttttctgat tacaggctgc
      121 ccgcttggct gtaaaggttg ccatagtgcg gatagctgga aagcctgtct tgggaaagag
      181 cttagcagtg aatatctctc tgaacgccta aaacgctacc aaggcttaat tagctgcgta
      241 ttgtttatgg gcggagaatg ggaagcagaa aaattactgc cgctgttgca actggcaaaa
      301 aaacaggggc taaacacctg tctttatagt ggtttggagc agcacgaaat cgccgagact
      361 ttattacacg agctaaccta cttaaaaacc gggcgttgga ttgccgaacg aggaggattg
      421 gaaagtttaa ccaccaacca acgctttact gatttacgca ataaccaaaa tcttaaccat
      481 ctttttattc gaggagcttc aatatgattc gcttacaacc gacacaatta gacggaaaac
      541 tacatttcat taaccaatat attcaagcca aaaatgccgc agacggttca aaaatggacg
      601 ccaacgctaa cgtcacgcaa aaaaacatag cgacaatgga acaggagcta atgaaagatt
      661 tttttgtgca aatcaaccga gcaaaagtga gtgaaaaaat tgcaaaaatt tttggaaaag
      721 aaaccgcttg tgaatattta cgtcaaatcg aagcacacga aatttacgta cacgatgaaa
      781 ccagcctaaa accctactgc gtttcagtca ctatgtaccc tttcttgcgt gatggtttga
      841 ctaaactcgg cggcgaatcc aaagccccga aacatttagc ctcattctgc ggttctttta
      901 tcaattttgt ctttgcagtc agttctcagt ttgccggtgc agtcgccacg gttgaatttc
      961 tgacctattt tgactatttt gcccgcaaag attacggcga taattactta cagactcatc
     1021 gttcagatat tgaaaaccac ctgcagcagg ttgtgtatag catcaaccaa ccggcagccg
     1081 ctcgtggtta tcaaagcgtc ttttggaata tttcagtgta tgacaaattc tattttgaag
     1141 cgatgtttag ccgttttgtt tttcctgatt ttgagaaacc aaattgggaa accacttcaa
     1201 aattacaact tttctttatg caatggttca ataaagagcg aagcaaagcg attctcactt
     1261 tccctgttgt gaccgcagca atgcttaccg aaaagggcaa atgtaaagat gacgagtttg
     1321 ccaatcaaat ggcaaaagag ctatctcaag gcaattcatt ctttatttat caatcagata
     1381 atccggattc ccttgcttct tgctgccgct tgagaaatga aatcagcgat catagcttct
     1441 cctattcatt aggggctgga ggtgttgcga caggttcaat caatgtaatt actattaata
     1501 tgaaccgttt ggtgcaagat aaccgtgatt taagcgaaga agtcagcaag atccataaat
     1561 atcaatacgc ttaccgcaaa ctgatggaag agtatttagc tgccggtatg ttgccggttt
     1621 acgatgccgg ttttatttca ctgcataaac aattcctgac tattggcatt aacggaatgg
     1681 cagaagctgc tgaatcacaa ggtttaaccg taggttacaa tccagactat attgattttg
     1741 tgcaatctcg gttaaaaatt attttccaag ctaaccaagc agccggtgaa aagtatgggg
     1801 taaaatttaa taccgaattt gtgcctgctg aaaatttagg ggtcaaaaac gccaaatggg
     1861 acaaagagga cggctatttc gtaccacgag attgctacaa ttcctatttc tatgtggtag
     1921 aagatgaaga aattaatgct ttggataaat tcttactgca cggcagagag ctgattgaat
     1981 ggctggacgg aggctccgct ctacacttga atttagacga agccttaacc caaaccggat
     2041 atcgctcctt gctggatacc gccgctaaaa ccggctgcaa ttatttttgt attaacgtta
     2101 aaatcacgat ttgcaacgaa tgtagccaca tcgacaaacg cacgttgcac agttgtagca
     2161 aatgtggctc cgataatatt gattacggca ctcgggtaat cggctattta aaacgagtct
     2221 ccgcctttag ttccgcccgc caaaaagagc acagcttgcg tttctatcac cgagaagcag
     2281 cttaattgaa aactgcagtt gtagcccttt acaaccgctt ttttagtgat tatattaaag
     2341 cctcatttag tcggggtgct tgcctaaggg caagctgaga aatacccgtt gaacctgatg
     2401 cagttagcac tgacgtagga aactaaaggc gtaaacccat ctattccccg ttggtgttgg
     2461 tgcgttgttt ttcaatcata ttaaggaata aacgatgacc acatcactcc cctatcactc
     2521 gcattatttg caaaaaattc gacaaaatcg accgcttgtt cacaacatta ccaacattgt
     2581 tgccgctaat ttttctgcca acggcttatt ggctcttggt gcttctccca ttatggcagc
     2641 tgctattgag gagatggaat ccgtgccggc actcagtaac gccgtggtga ttaatatcgg
     2701 cacgcttatc ggcaaagaag tagaagcaat ggagcttgct ggcaaaaccg ccaaccgttt
     2761 aggtattccg ctcattcttg accctgtcgg cgttggagca acacctttcc gccgtcaaac
     2821 agttgaaacc ttagtcgacc aaattcactt caccgccatt cgtggaaatg cgggggaact
     2881 agctaccctc gccggtgtag aatggcaagc caaaggcgtt gatgccggcg aaggctcgca
     2941 taatgtggtt gaaatcgccc aacagattgc caaacgttac caaaccttag tcgcaatcag
     3001 tggtgaaacc gatgtggtaa gctctggctc acacacagct ttattgcaca acggcagccc
     3061 gatgttcccc aaaattaccg cttcaggctg tttattaagt gcggtatgcg gggcattctt
     3121 agccgttgcc gacaaagagc actatttcaa cgcacttatt gaggcttgcg ccgcttatgc
     3181 cgttgcaggc gaagtggctg ctcaagcact caaaccaact caatacgggc aatttgccgt
     3241 caatctgctg gatacccttg gggcattaac gcctgaaacc gttgagcaat atgtgagggt
     3301 tgaatatgtc taaaactcac tcaaacccaa ctaaagtggc ggttgcctca atggtcggca
     3361 cggcgattga atattttgat aactacatct acacgatggc agcagtgctg gtgtttaatg
     3421 tgcagttttt taatagcgat gatccggttt ccaaccaatt aatgtcgctt tccgtactag
     3481 ctcttgcctt tttcacccgc ccgatggggt cgattttatt cgggcatttc ggcgataaat
     3541 acggacgcaa acaaactctg attgcctcgc tcttattaat gggcttatct accgtagcaa
     3601 tcgggctttt gcctaattat gccgccattg gcatttgggc aaccgtgtta ctctgcctct
     3661 gtagaatcgg acaaggttta ggcttaggcg gcgaatgggg cggagctgct ctcgttgcca
     3721 ctgaacacgc cccgaaacac aagcgggcat tttttggtat ttttccgcaa atgggcgccc
     3781 caatcggctt gctgttggca aacggagcgt tctacttggt gaatttaatt tacggtgaac
     3841 aaaccttctt ggattgggcg tggcgaattc cgtttgtcgc ttctattgtg atgatcgcca
     3901 ttgggcttta tatccgaatt aagatcagcg aaagcccgat tttccttaaa gcggaacaac
     3961 aaggtaaaaa ccgccatacc ccgcttaaaa ctttgtttag ccaacacctc aagcctttta
     4021 tgatcggcgt gctgattgcc accgccggct atgtgttgtt ctacatttta gttgctttca
     4081 ccccgatttt cgtcaaaagc ccggtggcag tttctcaagc aggacacgct acaggcttag
     4141 gtttaccggt aaatctctac acccaatatt tgctgattac ctccgtgatt ttcggcattg
     4201 cgattttctt ctccggcatt tattcagaca aaatcggacg ccgtttactg ctgttacttg
     4261 ccacaggagc aaccgtagtt tacggcttaa caatgccgtt tttcctcgaa aacggcacac
     4321 cgacttcgat ctttattttc ctcaccattg gaatgatctt aatcggctta agcttcggcc
     4381 cgatggcagt aattctaccc gaactcttcc caacagaagt acgttacaca ggggcttcgc
     4441 ttgcctacac cgctgccggt attttaggag ctagcgtgtt cactattatt gcggtcaaaa
     4501 tcaatgaaaa tttcggctta ttcggtgtag gcggctattt atcggttgcc tcattactga
     4561 gcttctgggc attgtggcaa gtccacgaaa ccaaagattt ggaattagag gagatataaa
     4621 aaaccactca cttaacgacg gggcaagtta ccaccttgcc ccaagatgag ggaatgcaaa
     4681 tttccctcta atagacttca caacggatat tttgcatttt tttataaaaa ataaccgctt
     4741 gtaagctata ctaaaaacgc ttgtcggagt gccgtaaggc tgagatcgca acagcgagat
     4801 ccgtggaacc tgtaggttag tacctgcgta gggaacaagt tttacccaaa ttcagatttt
     4861 gggatcatta ccacatctct ggcaagcaaa accctaagca tattatgagg attttgcgat
     4921 gtctaattat tcaaactctc cataccaacc taacgtcatt tggacggttg ccggctcaga
     4981 ttcctgtgcc ggtgccggtc tgcaaaccga tttacatacc tttcacgatt ttaacctagt
     5041 gggttgttca gtagttactt ctgtaacggc tcaacatccg cacggtgtgc tgtgtgtaac
     5101 gcctgttgat gatcacacct tccgccaaca gtttgaagcc ttgctggttc aaggctaccc
     5161 gaacgcaatc aaaatcggct tactctgctc gcaagcacaa gtagagatct tgtgtgaata
     5221 tatccaaaaa atccgtgcag aaagctcgca ttattgttat gtggtttacg accctgtggc
     5281 agtggcaagc agtgggcagg cgttatccga cagtatttta ttgcagatag tgcaacaaaa
     5341 attgtatccg ctggtggatt taatcactcc aaacggcact gaactcgctt tattaagcga
     5401 cacagaaatc cagacctttg gtgatgtgaa aaccgctagc cacaaactgt ttgcacaggg
     5461 aatcaaagcc gtgttagcca aaggtggaca ttttgaatgg ctgggggaaa cggtaagaga
     5521 ttatctgctg actgcaaatg aaatttatgc ctttagtcac gcccgacagc aaagtgtgaa
     5581 tacccatggc acaggttgca cctatgcttc agcggttgga gcgatgttgg caaccggctt
     5641 tgatttagct gatgccgtca ctgttgccac cgcttatttg caaaaaggct tatcggaaaa
     5701 acaaggcaga gggcaaactg ctttaagttc actttgccac accggctacc ctgatgatat
     5761 cgctttcttc ccacaaaccg agctgatttc tgcccctctt aaattgccga aagagccttt
     5821 tgctccaacg gaatatcaac ttgggcttta ccctgttgta gatagcgtta aatggcttga
     5881 acgcttaatt cttgtagggg taaaaacctt gcaaattaga atcaaaaatc gaccgcttgc
     5941 agagattgag caagaaattg cccattcggt cgcactggcg aaacagcaca atgtgcgttt
     6001 gtttgtaaat gattactggc agttagccat gaagtatcag gcttacggcg tgcatttagg
     6061 gcaggaggat ttagccaccg ccgatttaaa tgcaattcag caatctgggt tacggcttgg
     6121 tgtttcaacc cactgctact ttgaaataat gcgggcattg gcggtgaaac cgtcttatat
     6181 cgcttttggc cacgttttcc ctactcaaac caaagtaatg ccgtctcaac cacaaggctt
     6241 aacaaattta gccaaatatg ttgctctcgt tgcaccgctt aacattccaa ctgtggcgat
     6301 tggtggaatt aatgagcaaa gcattgaaaa tgtaatgcat accggtgtcg gcagtgccgc
     6361 ggtggtaagt gccgttaccc aagcaaaaga ttggcaacaa gcggtcaaaa acttagcaaa
     6421 atttgcaaat ggaggccgta atgaatagaa ctcttagtac gctaaacagt ggtttgattt
     6481 gggcgggagc gggcattagt gccagtgcaa tcgtggttgg cacttggatt gccccacttg
     6541 gctggcaaca aggcttgctg gcgatagtgc tagggcattt attaggcggc attttgtttt
     6601 ttgctgccgg cttaattggg gcgaaaaccg gcaaaaatgc gatgcaaacg gtgcaaattt
     6661 cctttggcag aggctcggtg tttttctccc ttgccaatgt gctacagctt gttggttgga
     6721 cagccattat gatctataca ggggcagaga tcagtaatgc ccttagcctt gccttatggg
     6781 aatattcggc tttctcgctt tgggcaatcg tgcttgggtt gctgattgtg ctatggttat
     6841 ttaccggctc taatcaggtc ggaaaattca agttagtgac attgatagcg atgtttttgc
     6901 tcaccttatg gctaagcatt aaggtattga atgggcagga aaattttcct gtttcgggtg
     6961 aaatgccgtt tagcaccgca gtggaattag caactattat gcctctttcg tggctacctt
     7021 tggtatcaga ctataccgct aaagcgaaaa agccttttgc cgcgaccctt tcggcgacca
     7081 taggctattt gatcaccagt gcgtggatgt atgcgttagg tctgggtatg gtgctattta
     7141 ccgggcaaac cgaaattgcc cctatgttac taatgacagg aatgagcctt atcggcatta
     7201 tggtgatgat tctttcgact gtgactacca cgtttcttga tgcctattcc gccggtgtca
     7261 gtgccggcaa tatcacggca aaattcaaag aaaccccaac cgccattcta gtcacaatca
     7321 tcggcacgct actggcaatc agtttaccgg ttacccaata tgaaaatttc ctgctgctga
     7381 taggctcggt attcgccccg atgattgccg tgcaaattgc ggatttcttt gtgttaaaac
     7441 gccaccaagc acagcctaat gttgattggg ttgctgccgg attatggctt gtcggcttcg
     7501 tgctatacca ccttctctct taccaccaag tgcatttact actcggctta accattccag
     7561 tgatgttagt gatttttctc agcaccattt ttgtgaggag gttcacaaaa tagaaagata
     7621 agcggttcaa ttggagtgaa aatttgctaa aattgagtcg cttgttttaa acaatgactg
     7681 ggtagtaaca ccacaagctc aacttgccta ccacaccatt aacggtaaag ccgatgagga
     7741 acggttaaat cttttagttg cccgtgccgg cgtaagagta gcaaaaggct ttgccttgaa
     7801 taatggttgg aatctgcaac cttatgcgga agtaaacggc attgctgaaa aaggccaata
     7861 gtacgaaagt gcaagtaaac caatatcagt ttggagttgc cgaaaacaag ggcagagtgc
     7921 aaggttcact tggcttgagt gcaggaaacg gtaatcaccg tttaggttta gaggcatcag
     7981 ttgcttcagg taagaaactg aaacaaccat ttaatgtatt agtaaattat cgttatcaat
     8041 ggtaatttaa gagggcaggt gaaaacctgc ccttaaattc aataattaat ctatcttccc
     8101 acaatttaaa cagtaaagat acatctgttt cggatcgtta aaacgtgaaa gagtctctag
     8161 ctgttgttgc ggttgcttca acagcggtaa atcaagccaa tttgccacgc taaattgtaa
     8221 ttgagctttg aaatcacggg caagcgtgcc aaataagctg tcatcttgct ccgcaaacca
     8281 ttgtaagccg aactgttcga ctttaggttg gttttccgct tcacgttttt ggttgatcaa
     8341 ttcagataac gcattatatg ccacattaaa attgcccaac tcatcaacca atccgtgttt
     8401 taacgcatca gttcccagcc acacttgccc ttgagccact ttatctacca cctcttttgg
     8461 cattttcctg ccacgagaaa ccagctctaa aaaacggtcg tagccgtttt caatcccaat
     8521 ttgcagcact tctgcctgct cttgcggtag gctttttaaa ccggcttgtt gtgctagggc
     8581 agaagtcgct atgccgtctt cgctcacacc gagattttta gcggtttttt caaaagttac
     8641 tgctaaaccg aaaataccga tagagccggt aagcgtattc gggctagcaa taattttatc
     8701 gctggtagcc gcaatccaat aaccgcccga tgctgccatt ccgcccattg aagcaaccac
     8761 cggtttgccg gcttgttgaa aggcttccac ttcttggcga atcagttcag acgccacagc
     8821 actgccgccg gggctgttaa tgcgtagaat taagccttcc acatttttgt catctcgagc
     8881 ttgttgcagt aattttacca gcgtctcgct gcccacattc atttcgtcac tttcaccgcc
     8941 agcaatcgcc ccttccacat tcactaccgc aattttcgga gcattcactt ctgcaaagcg
     9001 gtcgtttagt tcagtcatat aatctaaaaa ttcaatctgg ttataactgc cttcgctgtt
     9061 tttgccaaac tgtgcaatca gtttatcccg actttcttgg ctggacacta actgactaac
     9121 ccatttttga tttaatgcca gttgagcatc atctccttta gcttgtttat atttttcaat
     9181 gtaattggca ggcgataaat ccagtgcttg cggttcaatg ttgcggtttt gagctagtgt
     9241 tgccacagtt tgttgccaag tgccgtttaa ccagcctcgg gcattttgtt tcgcctcctc
     9301 agacatatca tcacgcataa acggctcaac cgccgattta taagtaccga cacggaaaat
     9361 atgcggttca gcctcaattt tatcgagcaa ggttttgaaa taagtgttgg aataatttaa
     9421 gccttttaaa tccaccgcac cgactttatt gagataaatt tcatcagcaa aggaagcaag
     9481 ataatattgt ttttgcgagt agtcttcgcc cactgcaatc accggcttgc ccgattgttt
     9541 gaagttgttg atttcattgc cgataaaatc caatgagctg aaatcagcac tttgtagctt
     9601 ttgcagatct aacaccaagc cggtaatttt cgggtcattt ttagcgtggc taatcgcttg
     9661 caccacatca aaagtcgaga ttttaaacgg ctcggaacta cctaattccg attgcaccaa
     9721 gcggtagaaa tcagtgaatt cttcccgatt atctgctaaa taaccatcta aatttaaacg
     9781 taacgcccct tgattgaaca ccggcttttg cgtgctgcca gaagagctga taaatgcgac
     9841 caccggcact aatagcaaaa taaacagaat aaaaaagaga ttaatgacac actctctaat
     9901 acaacggaag attcggtaaa atgcttttaa aatcgttaac atcggaaatt cctaaaaata
     9961 aactcagtcg gcacagtata aagcaatcgc taaatgaaga aaatatgcta ttcttgaacg
    10021 caaaaaatta acaaaggaac gcttatgcaa atcttggatt tattacacca tcgccgttca
    10081 aataaaaaat ttggcgataa agccccgaat gcagaacaac tggaacaaat tctaaaagcc
    10141 ggcttgcgag tgccggatca cgggcggtta aagccttatc gctttgtggt gattgaaaaa
    10201 agcggtatgc cacagtttgg tgaatgctta aagtctgcgg tagcagaatt tgatatgggc
    10261 gaggaacgct taaaaaaagc ggaaaaatta gctaatcaag ctccaatgat tatcggcgtg
    10321 gtagcaacat tagacgacag catcgccaaa gtgccaagtt gggaacaaat gattaccgca
    10381 ggctgtgcaa cttatgcgat tcagttagcg gcaaatgcac agggctttga tacggtttgg
    10441 atcaccaata aatggattaa cggctctgct cttcgctccg cttttggttg ccaagaatct
    10501 gataaaatca ttgccttaat tatgcttggc tcaccggcag agggcgagca aattgcatta
    10561 gcccctgaaa gtgaaagttt agaggggttt gtacgttatc ttaagtaagc aggttaacag
    10621 ataaaagtta gtaataaggg cgaaaaatat ttcgcccttg cttttatctg ttttcgcctt
    10681 acttcttcca gccttttaac gcatcggcaa aggcattgtt accaaaagcg ttgcgattgt
    10741 tgctttgcaa gcggtcattt ttctgcgtat ttttgcaaat ttctgctgaa tttcgaccgc
    10801 ttgttggctc ttttttatcg gttggtttgt cgtctaaacg catcgtaagg gcaatacgtt
    10861 tgcgggcagc gtccacctca agcactttga ctttcaccac atcaccagct ttcactaccg
    10921 tgtgcggatc ttcaacaaaa gagtttgaga gcattgaaat atgcaccaag ccatcttggt
    10981 gtacgccaat atcgacaaaa gccccaaagt ttgccacatt ggtcactgtg ccctccaaaa
    11041 tcataccggc tttgaggtcg gtaatttctt ccacgccgtc cataaacacg gcggttttaa
    11101 actctccacg agggtcacgc cccggttttt ctaactcttt gaaaatatcg ttcaccgttg
    11161 gtaaaccaaa ctgctcatcc acaaaatctt tggcgttgag cgagtgaatt ttgctgttgt
    11221 tacccattaa ttctgcaagt gttgaggtag ttgcctgtaa gattttttct accaccgggt
    11281 aagcttccgg gtgaacgctt gaggcatcaa gggcattttt cccaccgata attcgcataa
    11341 aaccggcaca ctgttcaaag gctttcggcc ctaaacgagg cacttttttc agctctgagc
    11401 ggctggtaaa acggccgttt tcgtcacgga atgccacgat attttgtgcc aatgttttgg
    11461 tcattcccgc cacccgagcc agcaacggag cggaggctgt attcagatcc acgcccactg
    11521 cgtttacaca atcttctact accgcatcga gtttacgggc aagttgggat tggtttacat
    11581 cgtgttgata ctgcccaaca ccaatcgctt ttggttcgat tttcaccaac tcggcaagtg
    11641 gatcttgcaa acggcgggca atcgaaaccg ctccccgtaa tgaaacgtct aaatttggaa
    11701 actcgttagc cgcaaattca gaggcagaat agaccgatgc cccagcctca ctcaccacca
    11761 ccgtttgcgg tttattctct ttgatttctt taatcaccgc tttggcaaaa cgttcggttt
    11821 cacgagaggc cgtaccgtta ccaatcgcaa tcagttccac attgtgttgt ttgattaagc
    11881 tgaaaatcgc aagctgtgcc tccgcctctc gcccagtgtg agggtaaatt gtggcggtat
    11941 ccagcaattt accggtgtta tccaccaccg ccactttcac gccggtacgc aagcccgggt
    12001 ctagccccat tgtatttctc gcaccggcag gggctgccat taataatgcc gagagattgc
    12061 gggcaaacac atcaatcgcc tcttcttcag ctttttcaca cagggttgcc attaattcgg
    12121 tttctaaatg cagagctgct ttgattttcc acgtccaagc aatcacttgc tcacgccatt
    12181 tatctgctgg ttgattatta aaaatcacgc ccaaatgctc acgaataatt tcttcacaat
    12241 ggctggattt tgtgccttct tctgcagtcg gatcggcatt taatgaaagt gataacaccc
    12301 cttcatttcg cccacggaac atcgccaaag cacggtggga aggcacgctg ttaaacggct
    12361 cgctatgagc aaaataatca cggaatttct cgccctcttc ctctttgccg tcaatcactt
    12421 tcgattccaa cgtggcgtaa gcggtcaaat attggcgaag ttttgcaagt agctcagcat
    12481 cttcgctaaa acgctccatt aaaatatatc ttgccccgtc taatgcggcc ttggtatccg
    12541 caacgccttt ttccgcatca acaaagtttg ccgctaaggt ttcaggatct tgtttcggat
    12601 cattccataa actgtccgcc aatggctcta accctgcttc aatcgcaatt tgccctctgg
    12661 tgcgacgttt tggtttatac ggcaaataga gatcttccag ctcggtcttg ctttcactgc
    12721 ttagaatttt ggcttttaat tcatcggtta atttgccttg ttcttcaatc gacttcaaaa
    12781 tggtttggcg gcgttcgtcc atttcacgca aataaattaa gcgggtttca aaatggcgaa
    12841 gttgggtgtc gtctagcccg ccggtcactt ctttacggta acgagcaata aatggaatgg
    12901 tattgccctc acctaatagc gtgatggctg caagaatttg gtgtgagcca acgttgagtt
    12961 cgccggcaat aatttggctg atttttagat ttaagtcagt cattagtttt ccttcaataa
    13021 atttgctcga ggtttatcca atttctcaag ctcggtcaat gtttcggcaa ttttttgcag
    13081 attcggctct tttttcgcac ataacaccat attttctaag ctatccgctt ggtgatagta
    13141 gggtgctttg cttttgacag cctgctcaaa ttgctgataa gcctgttgaa attgaccctg
    13201 tttacataaa aaagtgccgt aattattcag cacatcaggg cggttttgac tcaatttcag
    13261 tgccgtttgg taggcttttt cggcattttc agcatcacct gtttgttggt aataataggc
    13321 aagcaccgaa taagggaggt aatctttttc atcgtgagcc aaggctttat caatattctg
    13381 cttagctttt gggaaatcat tttgctccag ataagctaaa gccaaattaa tacgggcttt
    13441 taccgcttcc gagcggttaa attcgacttc agaggaagaa gggggaagag aagaacaagc
    13501 cgctaataaa aaaatcagaa tacaagcggt catttttgga aaaaattttg caaacataac
    13561 gttctcctac aacaaagggc ttaattttac tgttccatcc tcgctaagac tagcgatttt
    13621 ttgcgaagaa gaaaaatttt tcgttttcat ttttcgacgc agatcgcaaa aataataatg
    13681 gggtgttgtg c
//
LOCUS       DANLRK010000061        13665 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.061, whole genome shotgun sequence.
ACCESSION   DANLRK010000061 DANLRK010000000
VERSION     DANLRK010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 13665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13665
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.061"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(<1..1435)
                     /locus_tag="PXA33_001249"
     rRNA            complement(<1..1435)
                     /locus_tag="PXA33_001249"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            complement(1638..1714)
                     /locus_tag="PXA33_001250"
     tRNA            complement(1638..1714)
                     /locus_tag="PXA33_001250"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1678..1680),aa:Pro,seq:tgg)
     gene            complement(1739..1814)
                     /locus_tag="PXA33_001251"
     tRNA            complement(1739..1814)
                     /locus_tag="PXA33_001251"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1779..1781),aa:His,seq:gtg)
     gene            complement(1844..1920)
                     /locus_tag="PXA33_001252"
     tRNA            complement(1844..1920)
                     /locus_tag="PXA33_001252"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1884..1886),aa:Arg,seq:ccg)
     gene            complement(2079..2801)
                     /gene="rffM"
                     /locus_tag="PXA33_001253"
     CDS             complement(2079..2801)
                     /gene="rffM"
                     /locus_tag="PXA33_001253"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HDL5120584.1"
                     /translation="MFDKVIIRGIALQAVKNREIFANWLMNEKRIQFGRLVAINAEKV
                     ILSEKNPLLRRVIDEAEFNYADGISVVQSIRKKYPNYKIERIAGADLWETLMQRAGAL
                     NVPVFLVGSSADTLTKVEAKLAQWKVNIVGRQNGYFKAEQEQDVIEQIKASGAKFISV
                     AMGTPKQELFIQQLQQQYPQALYMGVGGTYDVFAGKVKRAPKIWQNLGLEWLYRLLKQ
                     PTRWQRQLNLLKYAYYYLTNQL"
     gene            complement(2791..4062)
                     /locus_tag="PXA33_001254"
     CDS             complement(2791..4062)
                     /locus_tag="PXA33_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WzyE family oligosaccharide polymerase"
                     /protein_id="HDL5120585.1"
                     /translation="MNNLIPLGGFYLVSVGLFFSLLYQLYQKSGISFHLLFSLLYFVT
                     FYAGFPFSLIMAFGFDYPLPELKTQWQVFTAAGVGYLIYYVVYSRLWSAVSQPTALVA
                     NFQAKTTACILAAVAVVALLYFLYLNGFLLFKLEKYSQLFAKDRVILSALKRFFYFLL
                     PALLIFCFLYKNKTAWWALLIVGGAFGILSYVAVGGTWANLALAVGFFLLIGLQQGYL
                     SLSKVIAVSLIGVVAMFFLALARYKLTLSGSEMIYTFLYLSRDTFSPWENLARVFSNE
                     VEYQGLMPIVRDFYVYIPQSLWQERPEIVWNTANYFTKVILGNQSGLAISPTLLGSFY
                     IMGGFSVMALGMGIVAAMIKALDNLFAYAKACNSALLQAYCFGNLFNLIVLVREGIDA
                     FVSRFCFFTLIFMACYLLAKILSMGKNNNVR"
     gene            complement(4055..5125)
                     /locus_tag="PXA33_001255"
     CDS             complement(4055..5125)
                     /locus_tag="PXA33_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HDL5120586.1"
                     /translation="MLPIYHILGSEIEHHNQQVLTFFQQELLPLLPDSEHFFYVVGKP
                     SLQTAFPHLRIKQFSSQKAIANALIAKKRQNPTACFLLHGQFNPWIWLAIALGRLPTT
                     NLIWHIWGADLYEMATSWKFKLFYPFRRVAQQKILQVWATRGDLDHLWRNMRTRSEQD
                     HLIYFPTKLGDQIKISPTQNDRLTILLGNSGDPANELIQALSEIHQRLGNNVRIIIPM
                     GYPENNESYIRQVEAYSKRLFLAENLQILRKKLSFEEYVTLLSHCDLGYFNFKRQQGI
                     GTISLLISHNVPCVLHSANPFGLDLQAEKVPYLLAENLTASQIQTTKQQLKSIDKNRI
                     AFFAPGYTKLWQQRLIELMQNE"
     gene            5312..6733
                     /gene="glnA"
                     /locus_tag="PXA33_001256"
     CDS             5312..6733
                     /gene="glnA"
                     /locus_tag="PXA33_001256"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011298219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HDL5120587.1"
                     /translation="MSNAIANVSKLIQDNDIKFVLLKFTDIKGKEHGVSLPVSLVAND
                     LEDLFEEGKMFDGSSVEGWKAINKADMLLMPIAETAVVDPFARIPTLSIRCSIYDPNT
                     MQSYDRDPRSIATRAENYLKSTGIADSVMFGPEPEFFLFDDVRFSTEMNNVSYRIDDV
                     EAAWNTNRKFEEGNKAYRPLKKGGYCAVAPIDTAHDIRSEMCLLMEEMGLVIEAHHHE
                     VATAGQNEIATRFNSLTLKADETQIYKYIVQNVAVEHGKTACFMPKPFAGDNGSGMHC
                     NMSLSKDGKNVFMGDKYAGLSETALYYIGGIIKHAKALNAFTNPTTNSYKRLVPGFEA
                     PVLLAYSASNRSASIRIPAVTSPKATRVEARFPDPMANPYLCFAALLMAGLDGVINKI
                     HPGDAMDKNLYDLPPEELKDIPTVASSLESALDALSNDFEFLTQGGVFTKDFIDAYIG
                     IRRKEVERVNMTPHPVEFELYYS"
     gene            complement(6777..7920)
                     /gene="rffA"
                     /locus_tag="PXA33_001257"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /pseudo
     CDS             complement(6777..7920)
                     /gene="rffA"
                     /locus_tag="PXA33_001257"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251256.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
     gene            complement(7907..8551)
                     /gene="rffC"
                     /locus_tag="PXA33_001258"
                     /gene_synonym="wecD"
     CDS             complement(7907..8551)
                     /gene="rffC"
                     /locus_tag="PXA33_001258"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HDL5120588.1"
                     /translation="MKPDQWLSDFFGREIVQVKVAADDFRQIARLQKQGFVLVEGELE
                     FELALATFSENMTACRPADMADLPDLNLLFGSAFPDSRFRSPYFSREENQRFYRTWIE
                     NAVKGKFDDIGLVQCNADGQIQGAVTVRLLAQQQAKIGLLAVKPDCRQQGIGSELLNA
                     AIAWAKQQNAKKLMITTQLSNQKAIRFYQAFGASIKAVYYWFYPALEKYDDEQS"
     gene            complement(8548..9795)
                     /gene="wecC"
                     /locus_tag="PXA33_001259"
     CDS             complement(8548..9795)
                     /gene="wecC"
                     /locus_tag="PXA33_001259"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HDL5120589.1"
                     /translation="MMSKTEFNTICILGLGYIGLPTSVVFANAGKQVIGVDINPQVVA
                     SLNQGNIHIIEPDLQTAFTQAVKKGNFFATAKPQPADAFIIAVPTPLCGKQPDLTYIQ
                     NAAAMIAPCLRKGNLVVLESTSPVGTTENLAEWLSQARPDLAFPADVSIAYCPERVLP
                     GNIMTELVENDRIIGGLSEQCSQQAVDLYQIFAKGRCVTTTARTAEMCKLVENSFRDV
                     NIAFANEISMICDALKINVWELIELANLHPRVNILQPGAGVGGHCLAVDPWFIVSTSP
                     QQARLIRTAREVNDSKPQWVIEQVKTSVADCAMEQDCKPSELTIACLGLAFKPNIDDL
                     RESPALAITRQLAAWHQGSLLAVEPNIQQLAGEKITLVDLNTALSQADILVLLVDHTS
                     FKQISREQIRQRHIIDCRGVWVR"
     gene            complement(9836..10648)
                     /locus_tag="PXA33_001260"
     CDS             complement(9836..10648)
                     /locus_tag="PXA33_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS chain length-determining protein"
                     /protein_id="HDL5120590.1"
                     /translation="MKTLFYRLLVLILFAAIGAGAGYGLSYTQPEKWKVTAQFEQPTI
                     PDLGNYYSLLSTYHFLNSNSQEANSQTAQQQASEQVYAEFKRNLTSADLLQAYLLQTE
                     AVKLKAQVAKKSPAVVAAELVEQFRFEKDMATSADNVSLIAENAEEAQKLLADFIPLV
                     NTQARDKLNADLIAKWKVLFQQIKTASELNINGKQGSQDWAAKLQMMRSVQPLDNQLT
                     AYRLVKSPSVPLKSESPDPPILADDWGIKWLTAGLYAQFCGESESSTCGTLI"
     gene            complement(10659..11723)
                     /gene="wecA"
                     /locus_tag="PXA33_001261"
     CDS             complement(10659..11723)
                     /gene="wecA"
                     /locus_tag="PXA33_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HDL5120591.1"
                     /translation="MWLTFLTVFIVSFLALIVMRPVAEFVGLVDKPNFRKRHQGLIPL
                     IGGIALFIGNLTFYFMQWQDMRLPELYLTAVTILLIIGVLDDRFDISPLLRAGIQAAL
                     AGAMIYSGLSLSSLGQIIAPFSIELGWLGIVFTVFITIGVINAFNMVDGIDGLLAGLS
                     SVSFAGLGTLMFINGEPSLGYWCFALIFVLLPYAMFNLSLFGAKWKVFMGDSGSMLIG
                     FTIIWILLLSTQGQGSPISPITGLWLIAVPLIDMVAVIIRRLKKGKSPFKPDRLHLHH
                     LMMRAGLTSRQALLVITLWAGFCSTIGVLGEVYYWNQWVMMLMFIGLFFLYAYSITHA
                     WRVTRFVRRLKRRAKRSKQL"
     gene            11865..13145
                     /gene="hemL"
                     /locus_tag="PXA33_001262"
     CDS             11865..13145
                     /gene="hemL"
                     /locus_tag="PXA33_001262"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005721785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HDL5120592.1"
                     /translation="MSISEQLFEQAQKVIPGGVNSPVRAFKGVGGTPVFIEKAEGAYI
                     TDSDGKQYIDYVGSWGPMVLGHNHPAIIDAVLKAVPKGLSFGAPTAAEITLAELVCKL
                     VPSIEMVRMVSSGTEATMSAIRLARGYTNRDKIIKFEGCYHGHSDSLLVKAGSGALTL
                     GQPSGPGVPADFAKHTLTCTYNDLDSVKAAFEQYPNEIACLIVEPVAGNMNCIPPQEG
                     FLQGLRDLCTQYGAVFIIDEVMTGFRVALGGAQSYYGVTPDLTTLGKIIGGGMPVGAF
                     GGKKEIMEYIAPTGPVYQAGTLSGNPIAMAAGLACLTELSKAGNETILAEKTKTLAEG
                     FKALADKHNVPLTVQYVGGMFGLFFTEQKAVNNFQEVMKCDAAKFNRFFHLMLEQGVY
                     LAPSAFEAGFMSLAHSDEDIARTLEAADKAFAQL"
BASE COUNT         3949 a         3281 c         2754 g         3681 t
ORIGIN      
        1 tacttctggt acaacccact cccatggtgt gacgggcggt gtgtacaagg cccgggaacg
       61 tattcaccgc aacattctga tttgcgatta ctagcgattc cgacttcatg gagtcgagtt
      121 gcagactcca atccggactt agacgtactt tctgagattc gctccccatc gctggctcgc
      181 cgccctctgt atacgccatt gtagcacgtg tgtagcccta ctcgtaaggg ccatgatgac
      241 ttgacgtcat ccccaccttc ctccggttta tcaccggcag tctcctttga gttcccgacc
      301 taatcgctgg caacaaagga taagggttgc gctcgttgcg ggacttaacc caacatttca
      361 caacacgagc tgacgacagc catgcagcac ctgtctcatg gttcccgaag gcactctcat
      421 atctctacaa gattccatgg atgtcaagag taggtaaggt tcttcgcgtt gcatcgaatt
      481 aaaccacatg ctccaccgct tgtgcgggcc cccgtcaatt catttgagtt ttaaccttgc
      541 ggccgtactc cccaggcggt cgatttatca cgttagctac gggcaccaga gttaaacccc
      601 aatccccaaa tcgacagcgt ttacggcgtg gactaccagg gtatctaatc ctgtttgctc
      661 cccacgcttt cgcacatgag cgtcagtaca ttcccaaggg gctgccttcg ccttcggtat
      721 tcctccacat ctctacgcat ttcaccgcta cacgtggaat tctacccctc cctaaagtac
      781 tctagttgtc cagtctgaaa tgcaattccc aggttaagcc cggggctttc acacctcact
      841 taaacaaccg cctgcgtgcc ctttacgccc agttattccg attaacgctc gcaccccccg
      901 tattaccgcg gctgctggca cggagttagc cggtgcttct tctgtgatta acgtcaatcg
      961 actgttctat taaaacaatc gccttcctcg tcaccgaaag aactttacaa cccgaaggcc
     1021 ttcttcattc acgcggcatg gctgcatcag ggttcccccc attgtgcaat attccccact
     1081 gctgcctccc gtaggagtct ggaccgtgtc tcagttccag tgtggctggt catcctctca
     1141 gaccagctag agatcgcggg cttggtgagc ctttacctca ccaactacct aatcccactt
     1201 gggctcatct tatggcaggt ggccaaatgg tcccaccctt tagtcctaag acattacgcg
     1261 gtattagcta ccgtttccag tagttatccc cctccataag ccagattccc aagcattact
     1321 cacccgtccg ccactcgtca cccaaggagc aaactcctat gtgctaccgt tcgacttgca
     1381 tgtgttaagc ctgccgccag cgttcaatct gagccatgat caaactcttc aattcaaaaa
     1441 gtttaatcgc tcaatactgc tgacataaaa tcgcacttcc aaatttaaac ctaattaaaa
     1501 ttaagttaaa agaaagtaat gaatttctag ttagcaccta ttaagacttc aaaattaaaa
     1561 aaatgttttt caatcaagtc aatcaacaag tgcccacaca gattgtctga taaattgtta
     1621 aagaacaaaa aataaattgg tcggcgagat aggatttgaa cctacgaccc actggtccca
     1681 aaccagttgc gctaccaagc tgcgctactc gccgtctgat actcacaaga gtattgaatg
     1741 gggtggctaa tgggattcga acccacgaca actggaatca caatccaggg ctctaccaac
     1801 tgagctatag ccaccattgt gttgatagtg ccgcattgac atctggcgcg ctcgacaaga
     1861 ttcgaacttg cgacctttgg ctccggaggc caacgctcta tccaactgag ctacgaacgc
     1921 ttcgatttac atcaattcaa cttaatgcgt cgcaacggag gcgtatatta tagatttcac
     1981 tttcccttgt ctagcacttt ttaaaaaaaa tgtaaaaaaa tgtattgttt ggttttattt
     2041 tattcaaaac gtttaataaa tatcaaaaaa tgcgtttttt agagctgatt tgtcagatag
     2101 taataggcat attttagtaa atttaattgt ctttgccagc gggttggctg tttcaatagt
     2161 cgataaagcc attccagccc caaattttgc caaattttag gtgctcgttt cactttaccg
     2221 gcaaacacat cgtaagtccc gccaacaccc atatataagg cttgtggata ttgttgttgt
     2281 aactgttgga taaaaagttc ctgttttgga gttcccattg ccacactaat aaacttcgcg
     2341 ccacttgctt taatttgctc tataacgtcc tgttcttgct ccgccttaaa atagccattc
     2401 tgcctaccga caatattcac cttccattgt gccaattttg cttccacctt cgttagggta
     2461 tccgccgagc tgccgaccaa aaacaccggc acatttaacg ctccggctct ttgcattaag
     2521 gtttcccata aatcggctcc ggcaattcgc tctattttat agtttggata tttttttcga
     2581 atcgattgaa caacactgat gccgtctgca tagttgaact cggcttcatc tatcactcgg
     2641 cgtaacaacg gatttttttc cgacagaatc actttctcag cattaattgc cactaatctt
     2701 ccgaactgaa tccgcttttc attcatcagc caatttgcaa aaatttcccg atttttgacc
     2761 gcttgtaacg caatgccccg aatgatcact ttatcgaaca ttattatttt tccccattga
     2821 aaggattttc gccagcaaat agcaagccat aaatatcagc gtgaaaaagc aaaaacggga
     2881 aacaaatgca tcgattccct ctcgcactaa tacgatcaaa ttgaataaat tgccgaagca
     2941 gtaggcttgc aacaaggcgg aattgcacgc tttggcgtaa gcgaataggt tatctaaggc
     3001 tttaatcatg gcagctacta tccccattcc caacgccata acgctaaatc cgcccataat
     3061 atagaaagag ccgagtaagg taggcgaaat agctaaccct gattggttgc ctaaaatcac
     3121 tttggtaaaa taattcgccg tattccagac gatctcaggg cgttcttgcc ataaagattg
     3181 tgggatatat acatagaaat cccgcacaat cggcattaaa ccttgatatt ccacctcatt
     3241 agaaaataca cgagccaaat tttcccaagg cgaaaaagta tctcggctta aatataagaa
     3301 ggtgtaaatc atctcgctac cggaaagggt aagtttgtaa cgggctagag cgaggaagaa
     3361 catcgccacc acgccgatta ggcttacggc aatcaccttt gataacgaca gatagccttg
     3421 ctgcaatcca atcaataaga aaaagccaac cgctagtgct aaatttgccc atgtgccgcc
     3481 cactgccaca tagcttaata tgccgaatgc cccaccaaca atcagtaacg cccaccacgc
     3541 tgttttattc ttatatagga agcaaaaaat taacaacgcc ggcaataaaa agtagaaaaa
     3601 tcgttttaat gccgaaagta tgaccctatc tttggcaaac agttggctat atttttcgag
     3661 tttgaaaagc agaaaaccgt ttaaataaag gaaatatagc agggcaacaa ctgccacagc
     3721 ggcaaggata caagcggtcg tttttgcctg aaaatttgca acaagagcag ttggttgcga
     3781 cacagcactc cacaagcgtg aatagacaac gtaataaatc aaatagccaa cacctgctgc
     3841 cgtaaaaact tgccattgtg tttttagctc cggcagcggg taatcaaagc caaatgccat
     3901 tattaacgaa aacggaaaac cggcgtaaaa agtaacaaaa tagagcaaac taaacagtaa
     3961 atggaaagaa atccccgatt tctgataaag ttgataaagt aagctgaaaa atagcccgac
     4021 agaaacaaga taaaatccgc caagtggtat taaattattc attttgcatt aattcgatta
     4081 atcgctgttg ccatagttta gtgtagcccg gagcaaaaaa cgcaatgcgg tttttatcta
     4141 tgctctttag ttgttgtttg gttgtttgaa tctgtgaagc agtcagattt tccgccaaca
     4201 aataaggcac tttttctgcc tgtagatcca acccaaacgg attggctgaa tgtaacacac
     4261 aaggtacatt atggctaatc aaaaggctta tcgtgccaat gccttgctgg cgtttaaaat
     4321 tgaaatagcc gagatcacaa tggctcaata acgtaacata ctcttcaaag ctcagcttct
     4381 tccgcaaaat ttgcaaattt tccgccaaaa ataaccgctt gctataggct tccacctgcc
     4441 gaatatagct ttcattattt tccgggtagc ccattggtat gataatccgc acattgttcc
     4501 ctagtcgttg atgaatctcg cttaaggctt gaataagctc attcgctgga tcgcctgaat
     4561 tgccgagcaa tatcgtcaag cgatcatttt gtgtggggct aatttttatt tgatcaccta
     4621 atttcgtggg gaaatagatt aaatgatcct gttctgatct tgtacgcata tttcgccaca
     4681 aatgatccag atcgcctctt gttgcccaaa cctgtaagat tttttgttgg gcaacacgcc
     4741 gaaacgggta aaacagctta aatttccacg aagttgccat ttcatataaa tccgcccccc
     4801 aaatatgcca aatcaggttg gtggtcggca atctgcctaa ggctatcgcc agccaaatcc
     4861 aagggttaaa ctgcccgtgt aacagaaaac aagcggtcgg attttgccgt ttttttgcaa
     4921 ttaaggcgtt cgcaatcgct ttttgtgaag agaactgctt aatccgcaaa tgaggaaaag
     4981 cagtttgcag tgaaggcttg cctaccacat aaaaaaaatg ttcggagtct ggcaataacg
     5041 gcaaaagctc ttgctgaaaa aaggttaaaa cctgttggtt atgatgctca atctcggagc
     5101 ctagaatatg ataaatcggc aacattacac gcttttctct ccccaccata caaacaatat
     5161 gcaagcggta agattttagc aaaaatttgc aacattactg caacttcttt tagctttgaa
     5221 acactaaaaa acatcacaca cttggttaaa aaatgctatg ataaccgagc ttttttatta
     5281 ttcatttatc ccaacgagga cttattttac tatgtcaaac gcaatcgcta atgtatctaa
     5341 actgattcaa gacaatgata ttaaatttgt cttattaaaa tttactgata ttaaaggtaa
     5401 agaacacggg gtttcgctcc ctgtcagctt agttgccaac gatttggaag acttattcga
     5461 ggaaggcaaa atgttcgacg gttcgtccgt agaaggttgg aaggcaatta acaaagcaga
     5521 tatgttgtta atgccgattg ctgaaaccgc tgtagttgac cctttcgccc gtattcctac
     5581 cctttctatt cgttgttcca tttacgaccc aaacacaatg cagtcttatg accgtgaccc
     5641 tcgctctatt gcaacccgtg ccgaaaacta cttgaaatcc accggtatcg ccgatagcgt
     5701 aatgtttgga cctgagcctg aatttttctt attcgatgat gtgcgtttta gcaccgaaat
     5761 gaacaatgtg tcataccgaa ttgacgatgt agaagcggct tggaatacca accgtaaatt
     5821 tgaagaaggc aacaaagcct atcgtccgtt gaaaaaaggc ggctactgtg cggttgcacc
     5881 gattgatacc gcccacgata ttcgctccga aatgtgttta ttgatggaag agatgggttt
     5941 agtgattgaa gctcaccacc acgaagttgc gacagccgga caaaatgaga tcgcaacccg
     6001 attcaacagc ctgaccttaa aagccgatga aacccaaatc tataaataca tcgtgcaaaa
     6061 tgttgccgta gaacacggaa aaaccgcttg ctttatgcca aaaccgttcg ccggcgacaa
     6121 cggctcggga atgcactgta atatgtcatt aagcaaagac ggcaaaaatg tgtttatggg
     6181 cgataaatac gccggcttat ctgaaactgc cctctactat attggcggta tcattaaaca
     6241 cgcaaaagca ttaaatgcct ttactaaccc aaccacgaac tcttataaac gtttagtgcc
     6301 gggttttgaa gcaccggtgt tattggcata ttctgcaagt aaccgctctg cctctatccg
     6361 tattccggct gtgaccagtc cgaaagcgac ccgtgtagaa gcacgcttcc cggatccaat
     6421 ggcaaaccca tacctttgct ttgccgccct gttaatggct ggcttagacg gtgtgatcaa
     6481 caaaatccac ccgggcgatg caatggataa aaacttatac gatttaccgc cggaagagtt
     6541 aaaagatatt ccaacggttg cctcgtcttt agaatccgct cttgacgcat taagcaatga
     6601 ttttgaattc ttaacacaag gtggcgtgtt cacgaaagac tttattgatg cttatatcgg
     6661 tattcgtcgc aaagaggtgg aacgtgtaaa tatgactccg cacccggtag aatttgaact
     6721 ttactattca tagtaacaac atatagcaaa aaacggctga aacactcagc cgttttttat
     6781 tgattgaaga aagcaagcac tgcttgaata acacgctctt gctcctgctt actcaaatta
     6841 taaaacagtg gcaatcttac caaccgttcg ctttctaagg tagtaaagcg atcatcacct
     6901 gctaatcgac ctaatttctg ccctgccgga ctgctgtgca acggcacata atgaaacacg
     6961 gttaagatat tctgtgcctt cataaaatca ataaaagcag ttctctgctt caagtcactt
     7021 agctttaagt agaacaaatg cccgttatgg cgacaatatt cgccaatgcg aggaagctcg
     7081 cacttagctg tttgggcaag tggcaaaaag gcttgatagt aattgtgcca aagggttaaa
     7141 cgctgttggt tgattttatc tgcctctata agctgagcat acaaataagc cgcttgaatt
     7201 tccgacatta aaaaacttga accaatatcc tgccagcgat atttatctac ctcaccacga
     7261 aaaaattggc tacggttagt gcctttctct ctgatgattt ccgctttttc cagcaaatgc
     7321 ctcattaatc acgatcgccc caccttcacc gccagcggta tagtttttgg tttcgtgaaa
     7381 actatagcaa ccaatatgcc cgatggaacc taacgcccta cctttataaa acgaattgac
     7441 tgcctgtgcc gcatcttcta ccacataaag cccgtatttt tcggctgttg ccattattga
     7501 atccatatca caagcaatgc cggcataatg caccggcaca atcactttgg tttttggggt
     7561 aatggcttgc tcaattttgc gttcatcaat attcattgta tccggacgaa tatccacaaa
     7621 cacaatcttt gccccacgca acgcaaacgc atttgcagta gagacaaaag tatagctcgg
     7681 cataatcact tcatcgtccg gctgaatatc cagcagtatt gccgccatct ctaacgcagc
     7741 cgtgcaagaa ggggtaagca acactttgct acgggccagg ttttcaagcc actgctcaca
     7801 taagcgggtg aatttaccac cgcccgaaag ctgccctgtt gccatcgcct gttctatata
     7861 ggcttgctcc gcccccacaa ccgggggttg attaaacgga ataaaatcag gattgttcat
     7921 catcgtattt ttccaaagca gggtaaaacc aataatacac cgccttaatg cttgcaccaa
     7981 acgcttgata aaaacgaatc gctttctggt tactcagctg agtggtaatc atcagttttt
     8041 tcgcattttg ctgtttcgcc cacgctatcg cagcatttaa caattccgag ccaatacctt
     8101 gttgtcggca atcaggcttt accgccaata atccgatttt cgcttgctgt tgggcaagta
     8161 agcgaaccgt taccgcccct tgaatttgcc cgtcagcatt gcattgaacc aagccaatat
     8221 catcaaactt gccttttacc gcattttcaa tccaagtccg gtaaaagcgt tgattctctt
     8281 cccttgaaaa atagggcgaa cgaaaccggc tatcgggaaa tgccgaacca aacaataagt
     8341 tcaagtcggg caaatctgcc atatccgccg ggcgacaagc ggtcatattt tcagaaaatg
     8401 ttgcaagtgc cagctcaaat tccaattctc cctccacaag tacaaaacct tgtttttgca
     8461 acctagcgat ttgcctaaaa tcatcagccg ctacttttac ttgcacaatt tctctaccga
     8521 aaaaatcgga cagccattga tccggcttca tcttacccac actcctcgac aatcaataat
     8581 atgccgttgc ctgatctgtt cccttgaaat ttgcttaaat gaagtgtgat ccactaacaa
     8641 gactaaaatg tctgcctgcg aaagtgcggt gttgagatcg actaaggtga ttttttcgcc
     8701 agccagctgt tgaatatttg gctcaaccgc caacaggcta ccctgatgcc aagcggcaag
     8761 ttgccgggta atcgctaatg ccgggctttc ccgcaaatca tcaatattcg gcttgaaagc
     8821 tagccctaaa caagcgatgg ttagctcact tggcttacaa tcttgctcca tcgcacagtc
     8881 tgcgaccgat gttttcactt gctcgattac ccattgcggt ttgctgtcat tcacttctcg
     8941 tgccgtgcga ataaggcgag cctgttgggg cgatgtggaa acgataaacc acgggtcaac
     9001 cgccaaacaa tgtccgccca cccctgctcc gggctggaga atatttactc tcgggtgaag
     9061 atttgccaat tcaattagct cccacacatt gatcttcaac gcatcacaaa tcattgaaat
     9121 ttcattggca aaggcaatgt tcacatcacg aaagctgttt tccaccaact tgcacatctc
     9181 cgctgtgcga gctgtggtgg tgacacaacg tccttttgca aaaatttggt ataaatcgac
     9241 cgcttgttga ctgcattgtt cgcttaatcc gccgataatg cggtcatttt caaccaattc
     9301 cgtcattata ttacccggca aaacacgttc agggcagtag gcaatagaca catcggctgg
     9361 aaaggctaaa tcagggcgag cttggcttag ccattcggct aaattttctg ttgtaccgac
     9421 aggcgaagtg gactccagca ccaccaaatt ccctttacgc aaacaagggg caatcatagc
     9481 ggcggcattt tgaatataag tgagatcggg ctgcttcccg caaagtgggg ttggcacggc
     9541 gatgataaaa gcatcggccg gttgcggttt ggcagttgca aaaaaattac cttttttgac
     9601 cgcttgtgtg aaggcagttt gtaaatccgg ctcgataata tgaatgttgc cttgattaag
     9661 gcttgccacc acttgcggat taatatccac cccaattact tgcttgccgg cattggcaaa
     9721 aacgactgag gtcggcaagc cgatatagcc taaccccaga atacaaatag tgttaaattc
     9781 agttttactc atcatcagtt acaaagagaa atggcacact aaattgtgtg ccattttaga
     9841 ttaaagttcc gcaggttgag ctttcgcttt caccgcaaaa ctgagcataa agcccagcag
     9901 taagccactt aatgccccaa tcatcagcca aaataggcgg atcaggcgat tctgatttaa
     9961 gtggaacgct aggcgattta accaagcgat aagctgtaag ttggttgtct aacggctgaa
    10021 cagaacgcat catttgcagt ttagctgccc aatcttgtga gccttgctta ccgttaatat
    10081 tcaactccga agcagtttta atttgctgga acaagacttt ccatttcgca attaaatcgg
    10141 cgtttaattt atctcgagct tgggtgttta ccaacggaat aaaatcggct aataattttt
    10201 gagcctcttc cgcattttct gcaattaagc tgacattatc tgccgaagtt gccatatctt
    10261 tttcaaaacg gaattgttca actaattcag ccgccaccac cgccggtgat ttctttgcaa
    10321 cctgagcttt taatttcacc gcttctgttt gtagcaaata ggcttgaagc aagtcggcgg
    10381 acgtgaggtt gcgtttaaat tccgcataaa cctgctcact tgcctgttgc tgtgccgttt
    10441 ggctattcgc ttcttggcta ttactattca aaaaatggta ggtggaaagt aacgagtaat
    10501 aattacctaa atcgggaata gtcggttgct caaattgagc ggtcactttc catttttcag
    10561 gttgagtata actcagccca taaccggcac ctgccccgat agcagcaaat aaaattagca
    10621 ctaaaagtcg ataaaataag gttttcacaa catatccttt aaagttgttt gctacgtttt
    10681 gcacggcgtt ttagacggcg aacaaaacgg gttacacgcc aagcgtgggt aatggaatag
    10741 gcgtatagga aaaacagccc gataaacatt agcatcatca cccactgatt ccaataatac
    10801 acctcgccta ataccccaat ggtggaacaa aatcccgccc agagggtaat caccaataat
    10861 gcttggcgag aggtaagccc tgctcgcatc attaaatggt gtaggtgcaa gcggtctggt
    10921 ttgaacggac ttttaccttt tttcaaacgg cggataatca ccgcaaccat atcaattaac
    10981 ggcacggcaa tcagccacaa accggtaatc gggctaattg gcgagccttg cccttgggtg
    11041 cttaacagca gaatccaaat aatggtaaag ccgatcagca tactgcctga gtcgcccata
    11101 aacactttcc atttcgcccc aaacaggctc aaattgaaca ttgcatacgg caacaacaca
    11161 aagataaggg caaagcacca atagcccaaa cttggttcgc cattaatgaa cattaaagtg
    11221 ccaagaccgg caaaactcac gctggaaagc cctgctaata agccatcaat cccatctacc
    11281 atattaaacg cattaataac gccaatggtg ataaacacgg taaagacaat gccgagccaa
    11341 cctaattcta tactaaaggg ggcaataatt tgccctaaac tgctgagcga caagccactg
    11401 taaatcatcg ccccagctag tgctgcttga atcccggcac gcagtaatgg gctgatgtca
    11461 aatcggtcat ctaacacgcc gataatcagc aaaatagtta ccgctgtcag atacagttcc
    11521 ggcagccgca tatcttgcca ctgcataaaa tagaaagtga gattgccgat aaataatgca
    11581 ataccaccga ttaacggaat caacccctga tggcgtttac ggaagttggg cttatctacc
    11641 aaaccaacaa attccgccac cggacgcatc acaatcaggg ctaaaaaaga aacaataaat
    11701 acggttaaaa aagtaagcca catacaggga ttgcctcaaa aaatgagtca taaaatagga
    11761 atgcagaata gcataaggca acaaaatcgt aaatttttta gcgaaaacaa gggcttttca
    11821 gtacaataac aggcaaattt ttacatcaaa agaaaggaat tgatatgtct atttcagaac
    11881 aactgtttga acaagcccaa aaagtgattc cgggcggtgt gaactcccct gtgcgtgcat
    11941 ttaaaggcgt gggcggcaca ccggttttta ttgaaaaagc cgaaggtgct tatattaccg
    12001 acagcgacgg caaacaatat atcgactatg tcggctcttg ggggccaatg gtactcgggc
    12061 ataaccaccc ggcgattatt gatgcggtgc taaaagccgt gccaaaaggc ttaagtttcg
    12121 gtgccccaac ggcagcagaa atcacacttg ccgaattagt gtgcaaatta gtaccttcta
    12181 ttgaaatggt gagaatggtg agcagcggaa cagaggcaac aatgtctgcc attcgtttgg
    12241 ctcgtggcta taccaaccga gataaaatca tcaaatttga aggctgctac cacgggcatt
    12301 ccgattctct gttagtaaaa gccggttcag gtgctttaac cctcggtcag ccaagcggcc
    12361 cgggcgtacc cgctgatttc gccaagcata cgcttacttg cacctataat gatttagatt
    12421 cagtaaaagc agcatttgag caatacccaa atgagatcgc ttgtttaatt gttgagcctg
    12481 ttgccggcaa tatgaactgc attccgccac aagaaggctt tttacaaggc ttacgtgatc
    12541 tttgcacaca atatggtgcg gtatttatta ttgatgaagt aatgaccggc ttccgtgtcg
    12601 cactcggtgg ggctcaatct tactatgggg taacaccgga tttaaccaca ctcggcaaaa
    12661 ttatcggcgg cggaatgccg gtgggggcat ttggtggtaa aaaagagatt atggaataca
    12721 tcgctccaac tggcccggtt taccaagccg gcacgctctc aggcaacccg attgcaatgg
    12781 ctgcaggttt ggcatgctta actgaattat caaaagcagg aaatgaaacc attttagcgg
    12841 aaaaaaccaa aaccctcgct gaaggcttta aagctcttgc agataagcac aatgtgccat
    12901 taacggtaca atatgtcggc ggaatgttcg gcttattctt caccgaacaa aaagcagtaa
    12961 acaacttcca agaagtaatg aaatgtgatg ccgccaaatt caaccgtttc ttccacttaa
    13021 tgctggaaca aggcgtgtat ttagccccat cggcattcga ggcagggttt atgtctttag
    13081 ctcacagtga tgaagatatt gctcgtacgc tagaggctgc ggataaggca tttgctcagc
    13141 tttagaagaa tgacaagcgg ttgggtttac tcaataattt gcaagttttt gagggaattt
    13201 gaccgcttgt agtgcaaatt tctcttacga tagcaattat gatggtgttc gtctctgacg
    13261 agtgcttata caaactttca ttatgcttaa acaagcaccc gtctggagac gggcgctatc
    13321 gagggaatag gattgataaa gcaatatatg ggcttccaac acttgcaggc aaggacaagt
    13381 cttacacttt ctaatattac aaatatattt ctttctttaa tctataccat aaaggtttaa
    13441 gttggttctt tataactttg gaatccatag atgaataagc tctctttcca taagagataa
    13501 aattagattt tccgaaatga gaatacttag aaaatagctt tcttttataa atgcctttga
    13561 tagctacccc tcgtcttatt cttatcctat tatatttacc ctgagtttgc ttagctagac
    13621 taacccctcg tttcatttta tttgaatatc gtgtaaatgc cgatg
//
LOCUS       DANLRK010000062        13612 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.062, whole genome shotgun sequence.
ACCESSION   DANLRK010000062 DANLRK010000000
VERSION     DANLRK010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 13612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13612
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.062"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            56..1009
                     /locus_tag="PXA33_001263"
     CDS             56..1009
                     /locus_tag="PXA33_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL5120593.1"
                     /translation="MEIILRLLWRRLMALPVMILGVTALVFIVLQFTPGDPATVALGE
                     SASEAAKEIYREQHGLNDPLLVQYFRFLGNLFVLDFGMTTPPEQPIVDMIAKAFPLTL
                     QLTFIGVFLAAIVSFTLGVTAALYRDTWIDQLIRLISVAAVATPSFWLGILLIQYFSL
                     KLDWLPSGGFIPFSEDPKGYVNSMVLPSLALAVPVCASLIRVVRTTMVEEMDKDYVRT
                     AIGNGVPYSTVIRHNVLRNALITPVTVLGLRVGYLLGGAVVIEQIFDLPGMGKLIFNG
                     IVNHDLNLVQGVVLTIAFTFVLVNIIVDILYLLINPKIRSL"
     gene            1011..2981
                     /locus_tag="PXA33_001264"
     CDS             1011..2981
                     /locus_tag="PXA33_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/oligopeptide/nickel ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HDL5120594.1"
                     /translation="MFRQGLAARLASGGARFRALSTSSKIALLFIVFIALVAIFAPLV
                     APYDPLQTLRPVQAPSGDYLFGTDRLGRDIFSRMVWGARTSLFIGLGAVGVAILFGSI
                     LGATAATADKFGNEVIMRFMDILMAFPGIALAAVLLATFGNSVPVIIVTIAVVYTPQL
                     ARVVRANVVSQWEEDYVRAERVIGGSRTYILLKHVVRNTAAPVLVFATVMVADAIVFE
                     ASLSFLGAGVQPPFPSWGNILSEGRNLVLSGFWWATTFAGIMILLTVLALNILAEGLT
                     DALVNPKLKRSTQSKEDVAKPLATDVQEAMSETLALKRYLLKLHEKETTRTDRMQLNS
                     TAKPILQVKNLSIRFPNRYGETPLVDNISFTVHEGETMGLVGESGCGKSITAFSIMGL
                     LPKTAKITGEILFTDRSGKQHNLLESADLNALRGHEISMIYQDALSALNPSMRIKDQM
                     AQLISRGGKQSAETLLQWVKLDPEKTLNRYPHELSGGQRQRVLIAMALAREPKLLIAD
                     EPTTALDVTVQAEVIKLLNELREKLGFAMVFVSHDLALVAQIAHHITVMYAGQVVETA
                     PTTPLLANPTHEYTRGLLGSVLSTELRAERLYQIPGSVPSPFDFAKGDRFASRSLRPD
                     ANPEQHLKLVPTGDHPQHFWASHLEDPQSKEA"
     gene            2996..3802
                     /locus_tag="PXA33_001265"
     CDS             2996..3802
                     /locus_tag="PXA33_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL5120595.1"
                     /translation="MSMKFIKEQPIIELSNIVVQFASRDGTLFNPKKFTAVNDVSLAI
                     HAGETVGLVGQSGCGKSTLASVMIGLQKPTSGTVKFNGLEMKYGSAEARKYFGSQVSV
                     IFQDPATALNPRMRVLDILKDPMDIHNILQPQEREKRVYDLLSRVGLPRSAALVEPTR
                     LSGGQKQRVAIARALALNPKLIVADEPTSALDVSVRAQVLNLLADLKKELNLAMVFIS
                     HDLQTVHQVSDRIVVMNGGKIVEMGDASDVFERPTQEYTRTLIEAAPSLL"
     gene            3865..5451
                     /locus_tag="PXA33_001266"
     CDS             3865..5451
                     /locus_tag="PXA33_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HDL5120596.1"
                     /translation="MIQITQTINIDLLIVGSGIAGLSAAVEAKEKGINTLLISKSTIG
                     SGASYFPLKATLGIQVTGDEADKIHFKEDIENIGKGKINPNVVQAYIEDSPQAIELLH
                     RIGFQPWKRNDNRPACFAKYPRPIYLIKDWRESALRAKQILQEQHIAFYENATLLHIV
                     TDNNQVQGAVFSLTTSGQISYIFCKTKHLILAAGGIAGLYKDNLYPADVIGSLHYVAQ
                     QAGATLVNLAFIQFIPAFVEPKYKVLFGEHTLKYVTQITDSKGNNLFPHLNAEQFAKM
                     VLERSHYAPFSLDFAGVEFDLVMMKHLLENPHEKGIYLKYSPKLYEDTEEFYTVYLNW
                     LKNEVGIDLVQDKIAIKPFAHSCNGGIQINQWGESAVKGLFAIGEISHCIEGANRMGG
                     NSVGGGLVFAKRAVSEIVRSLEQNDLQKNSEISPLAMAEKALNQLANSTANLELTASE
                     VLSTIREKMALYANVYRTRSNLKLLLEELNRLEQEFDPIYHAQYQGIEIYNALKTAQL
                     VVNQMLNEQSLGAHYLDEQN"
     gene            5497..6879
                     /gene="nhaC"
                     /locus_tag="PXA33_001267"
     CDS             5497..6879
                     /gene="nhaC"
                     /locus_tag="PXA33_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDL5120597.1"
                     /translation="MNNYLLKLKPMTAVILSLLTVFILGFTMIGLGWVPQISILAVIV
                     MLLLFGLINKIPFKNMQEGMASGLMSSIGAVYLFFFIGLLVSALMMSGAIPTLMYYGL
                     SVISPELFYISAFALSSVVGIALGSSLTTTATIGVAFIGMASAFGANPAIVAGAVVSG
                     AFFGDKTSPLSDTTTIAASVVGIDMFAHIRNMLYTTIPAWILTALFLWFLTEPTDAQN
                     LANIENLKTNLQETGLIHGYSLLPFVTLILMAIKRVNSIYIIVTTVTLSLIITYIHSS
                     PTLEQLGNYFFTSYKPVEGVDLGEVASLVSRGGINSMFFTITIVLLALSLGGLLQILG
                     ILPALLNGISGFLTSTGRVTLVVASTAVGINVLIGEQYLSLLLSGNTFRSTYERLGLE
                     VKNLSRTIEDAGTVINPLVPWGVCGVFISSVLNVPVLDYLPYAFFCYICLILTLIFGF
                     TGVTISKKAN"
     gene            complement(6922..8118)
                     /gene="ispC"
                     /locus_tag="PXA33_001268"
     CDS             complement(6922..8118)
                     /gene="ispC"
                     /locus_tag="PXA33_001268"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005620038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HDL5120598.1"
                     /translation="MKKLVILGSTGSIGKSTLSVVEHNPNPNQYEVFALVGGKNVELM
                     LEQCRQYQPTFAAMDDEKSAQALKTALATLGSKTEVLAGQAAICELAAHSEVDMVMAA
                     IVGAAGLLPTLSAVKAGKTVLLANKESLVTCGQIFIDEARKSGAKLLPVDSEHNAIFQ
                     SLPPEAQQKVGFCPLAELGVSKIILTGSGGPFRTKPLNEFDAITPAQAVAHPNWSMGK
                     KISVDSATMMNKGLEYIEARWLFNATAEEMDIIIHPQSIIHSMVRYIDGSVIAQMGNP
                     DMCTPIAHTMAYPNRINAGVPPLDFFKLKELTFIEPDFARYPNLKLAIEAFAEGQYAT
                     TAMNAANEIAVEAFLNEQIRFTDIVNVNRTVVENIAPIQVSEIADVLHIDKLARELAK
                     QAVLQL"
     gene            complement(8237..10435)
                     /gene="relA"
                     /locus_tag="PXA33_001269"
     CDS             complement(8237..10435)
                     /gene="relA"
                     /locus_tag="PXA33_001269"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HDL5120599.1"
                     /translation="MVAIRYSHQLEPNNFELSSWCASLNMSPVTFEQIHNAWSYAQEK
                     LDTDYSLMWYGIEMVEVLHGLNMDDDSLVAAMLFPLVKHNVIDLVQLKEDFSNDIKNL
                     VKGVLEMENIRQLSANNVSDLQIDNIRRMLLAMVDDFRCVVIKLAERIAYLRDSNHHS
                     EEDTVLAAKECSHVYAPLANRLGIGQLKWELEDYCFRILHPVSYTQIAKFELGERRVD
                     RERYIANFVADLTACLSPEIKNVQIYGRPKHIYSIWKKMQKKRLRFNQLFDIRAVRVI
                     VPTLEDCYTALGIIHTKYTHLPEHFDDYIANPKPNGYQSIHTVVLGEGEKTIEVQIRT
                     QQMHDDAELGVAAHWKYKEGATAGRSGYEEKIVWLRKLLEWQNDLADSGDMVAEMRSQ
                     IFDDRVYVFTPRGEVIDLPQKATPLDFAYAIHSEIGHRCIGAKVAGRIVPFTYHLQMG
                     DQVEIITQKEPNPSRDWLNPNTGFVNTGKARSKIIAWFKKLDREKNIPIGKEALEAEM
                     EKFGFTLKQIEEYALPRYNLKQFDDLYAGIGGGDIRLHQLMHFLQSKLIKPTAEQEDE
                     AVLKQVNKNTNQPKPSKGYVIVEGVGNLMHSIARCCQPIPGDEIVGYITQGRGISIHR
                     ADCEQLFELQSLNPERVVEADWGEGYTSGLSLTIRVIANDRNGLLRDVSAIMANEKVN
                     VLGVSSRTDVKRSLATIDMEIQLNNVEILNKLLARIAQLDDVIEAKRLSS"
     gene            10689..11996
                     /locus_tag="PXA33_001270"
     CDS             10689..11996
                     /locus_tag="PXA33_001270"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HDL5120600.1"
                     /translation="MTILALGINHKTASVGLREKVAFVDDKRKLALEQIQTSGLAESV
                     VILSTCNRTELYFHQPNISPREESEENIQWREQCFRWFAQIHQLDESELRECLYFKQN
                     LEAANHLMKVASGLDSLILGEPQILGQVKQAYQYSEDFYQHQQKAMSTKLSRLFQRTF
                     ATAKRVRSETEIGSQAVSVAYAACGLARQIFDNFGKLRFLLVGAGETIELVARYLIQH
                     GAKNIMLANRTHIRAEMLAEKLNAPMQILSLSALQLGLNQADVVISSTGSPDTLISKE
                     MVEIAQKQRQFDPLLLIDIAVPRDIDEKAGDLEGVYAYSVDDLQNIIQRNMAQREEAA
                     KQAAEIVKEESKAFFEWLKQQQSSDLIKRYRQDAEQTRQELLEKALQAISQGQDSQKV
                     LSELSYKLTNQLLHAPTQALQAMAKSGNSTGLQSFSRALRLEE"
     gene            12181..>13612
                     /gene="der"
                     /locus_tag="PXA33_001271"
     CDS             12181..>13612
                     /gene="der"
                     /locus_tag="PXA33_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HDL5120601.1"
                     /translation="MTPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGH
                     ANIAGYDFIVIDTGGIDGTEEGVEEKMAEQSLLAIEEADVVLFLVDARAGLLPADVGI
                     AQYLRQREKTTVVVANKTDGIDADSHCAEFYQLGLGEVEQIAAAQGRGVTQLIEQVLA
                     PLGEQLNAEQFNQEQAGENAKNVANEETDEWDTEFDFENEEDAALLDEALAEEAEESI
                     EDKNIKIAIVGRPNVGKSTLTNRILGEERVVVYDMPGTTRDSIYIPMERDGQQYTIID
                     TAGVRKRGKVNLAVEKFSVIKTLQAIQDANVVLLTIEAREGISDQDLSLLGFILNAGR
                     SLVIVVNKWDGLSQDIKDQVKSELDRRLDFIDFARVHFISALHGSGVGNLFDSVKEAY
                     ACATQKTSTSMLTRILRMAADEHQPPLVNGRRVKLKYAHPGGYNPPIIVIHGNQVEKL
                     ADSYKRYLSNYFRKSLKIIGSPIRILF"
BASE COUNT         3870 a         2936 c         2807 g         3999 t
ORIGIN      
        1 ttgtaggtaa acttagattt aattttggag tgtaactaca ctcaaggaga agaaaatgga
       61 aattatactc cgattattat ggcgtcgcct aatggctctg ccggtaatga tcttaggcgt
      121 aactgccttg gtatttattg ttttacaatt cacccccgga gatccggcaa ccgttgctct
      181 cggtgaaagc gccagcgaag ccgctaaaga aatctatcgt gaacaacatg ggttaaatga
      241 tccattgctt gtacaatatt ttcgcttttt agggaattta ttcgtattag atttcggtat
      301 gacaacccct ccagaacagc ctattgttga tatgattgct aaagcattcc ctttaacatt
      361 acagcttacc tttattgggg tcttcttagc tgctatcgtc tctttcacat taggtgtaac
      421 ggcagcactt taccgtgata cttggattga tcaattaatc cgtttaatct cggttgccgc
      481 tgttgccaca ccttctttct ggttgggtat tttattgatt caatacttct ccttaaaatt
      541 agattggttg ccttcaggcg gttttattcc atttagcgaa gatccaaaag gctatgtaaa
      601 ctcaatggtt ctcccttcac tcgctttagc tgttcctgta tgtgcctcat tgatccgtgt
      661 cgtgcgtaca acaatggtag aagaaatgga taaagattat gttcgaacag ccatcggtaa
      721 tggtgtacct tactcaacgg tgattcgcca caacgtgctt cgtaatgcct taattactcc
      781 ggtaacagta ttaggtttac gggttggtta tttattaggt ggtgcagttg tgattgaaca
      841 gatcttcgac ttaccgggaa tgggtaaatt aattttcaac ggtatcgtca atcacgactt
      901 gaacttagtg caaggtgtag tattaactat cgcatttact ttcgtgttgg tgaatatcat
      961 tgttgatatt ctttacttgc tcattaatcc aaaaattcgg agtttataat atgtttcgcc
     1021 aaggattagc tgctcgactt gcttcaggcg gtgcaagatt tagagcatta tcaaccagct
     1081 caaaaatcgc cttactcttt attgtattta tcgcattagt cgctattttt gcaccattag
     1141 tagcgcctta tgacccactg caaacgctac gtccagtgca agctccaagc ggtgattatt
     1201 tattcggtac agaccgttta ggacgtgata ttttctcgcg tatggtatgg ggagcaagaa
     1261 catctctctt tatcggttta ggtgcggtag gcgttgcgat tctattcggt agtattttag
     1321 gggcaactgc ggcaaccgcc gataaattcg gtaacgaagt gattatgcgt tttatggata
     1381 tcttaatggc attcccgggt atcgcattag ctgctgtatt acttgccact ttcggtaact
     1441 ctgtacctgt gattatcgtg actattgcgg ttgtttacac tccccaactt gcccgagtgg
     1501 tgcgtgctaa cgtagtatct caatgggaag aagattatgt gcgtgccgag cgtgtaattg
     1561 gtggtagccg tacttatatt ctattaaaac acgttgtgcg taatactgct gcaccggttt
     1621 tagtattcgc aacagtgatg gttgctgatg ccatcgtatt tgaagcatca ctttcattct
     1681 taggtgcagg tgtccagcct ccattcccat cttggggaaa tatcttatct gaaggtcgta
     1741 accttgtatt aagtggcttc tggtgggcta caactttcgc agggattatg attttattaa
     1801 ccgtacttgc gttaaatatc cttgctgaag gcttaaccga tgcgttagta aatccaaaac
     1861 taaaacgttc aactcaaagt aaagaagatg tcgcaaaacc attggcaaca gacgttcaag
     1921 aagcaatgtc tgaaactctt gcgttaaaac gttacttact caaattacac gagaaagaaa
     1981 cgacccgtac tgaccgtatg caattaaact caacggccaa accgatttta caagtgaaaa
     2041 acttatcaat ccgtttccca aatcgttatg gtgaaactcc gcttgttgat aatatcagct
     2101 ttactgttca cgaaggcgaa accatgggct tagtgggtga atctggttgt ggtaaatcaa
     2161 tcaccgcatt ctcaattatg ggtttattac caaaaactgc aaaaatcact ggtgaaatcc
     2221 tctttaccga ccgttcaggt aaacaacaca atcttttaga atcagccgat ttaaatgccc
     2281 ttcgtggtca cgaaatctcg atgatttacc aagatgcatt aagtgcgtta aacccatcaa
     2341 tgcgtattaa agaccaaatg gcacaattaa tcagccgtgg tggtaaacaa tcagcagaaa
     2401 cattattgca atgggtaaaa cttgacccgg aaaaaacgct caaccgttat cctcacgagt
     2461 tatctggagg acaacgtcag cgtgtattaa ttgcaatggc acttgctcgt gaaccgaaac
     2521 tattgattgc tgatgaacca acaacagcat tagacgtaac cgttcaagct gaagtgatca
     2581 aattattaaa cgaattacgt gagaaacttg gatttgcaat ggtattcgtg agccacgact
     2641 tagctttagt ggcacaaatc gctcaccaca tcacggtaat gtatgctggt caagtggttg
     2701 aaactgcacc aactacgcca ttattggcaa acccaacaca cgaatacaca cgtggattat
     2761 taggctcggt actttctacc gaattgcgtg cagaacgcct ataccaaatt ccgggcagtg
     2821 tgccttcacc atttgatttt gcgaaaggtg accgttttgc gagtcgttca ttaagaccag
     2881 atgcaaaccc tgaacagcac ttgaaattag tgccaaccgg tgatcaccca caacacttct
     2941 gggcttcaca cttagaagat ccacaatcta aagaagcata aaggagagca atacgatgag
     3001 tatgaaattt atcaaagaac agccaattat tgaactgtct aatattgttg tgcaatttgc
     3061 ttctcgtgac ggcaccttat ttaacccgaa aaaattcacg gcggtaaatg atgtcagcct
     3121 agcaattcac gccggtgaaa cagtcggtct ggttggacaa tctggttgtg gcaaatctac
     3181 tttagccagt gtgatgatcg ggcttcaaaa accaacctcc ggcacagtaa aatttaacgg
     3241 cttagagatg aaatatggca gtgcggaagc tcgcaaatat ttcggtagcc aagtgtcagt
     3301 aattttccaa gacccggcaa ccgccctaaa tccgcgtatg cgtgtgttgg atattttaaa
     3361 agatccgatg gatattcata atatcttaca accgcaagaa cgtgagaagc gggtttatga
     3421 tttgctctct cgagtcggtc tacctcgttc agcggcgttg gtcgagccaa cccgattatc
     3481 tggtgggcaa aagcaacgtg tagcgattgc tcgtgcattg gcattaaatc cgaagctgat
     3541 tgtggctgat gaaccaacct ctgcacttga cgtatcggtt cgagctcaag tactgaacct
     3601 gctggcagac ttgaaaaaag aactcaattt agcgatggta tttatctctc acgacttaca
     3661 aaccgttcac caagtttcag accgcattgt ggttatgaac ggcggtaaaa ttgtggaaat
     3721 gggcgatgct tcagacgtat ttgaacgccc aacgcaagag tacacccgaa ccttaattga
     3781 agcagcacca tctttattat aattgtgctt tgtgcctaca agcggtaaaa tttttcgcaa
     3841 attttaccgc ttgtgttttt atttatgatc caaattaccc aaaccattaa cattgattta
     3901 ttaattgtcg gctctggcat cgctgggctt tctgctgcag ttgaggcaaa agaaaaaggc
     3961 attaatacct tactgattag caaatctacg attggctcgg gagcgagcta ctttccgctt
     4021 aaggcaacac ttggcattca agtaaccggc gatgaagccg ataaaattca ctttaaagaa
     4081 gatattgaaa acatcggcaa aggcaaaatc aaccctaatg tggtacaagc ctatatcgaa
     4141 gattcaccac aagcgattga actactccac cgtatcggct ttcagccttg gaagcgtaac
     4201 gacaaccgtc cggcttgttt tgccaaatac cctcgcccaa tttatttaat taaagattgg
     4261 cgggaatctg ccctacgagc gaaacagatt ttgcaagagc aacacattgc tttctatgaa
     4321 aatgctaccc ttttacacat cgtcacagac aataaccaag tgcaaggtgc ggtattttcg
     4381 ctcactacaa gcggtcaaat ttcctatatt ttttgcaaaa ccaagcatct tattttagcc
     4441 gcaggcggga ttgctgggct gtataaagat aatctctacc cggctgatgt tatcggttca
     4501 cttcattatg tggcacaaca agccggggca accttggtta atcttgcctt tattcagttc
     4561 attcctgcct ttgtcgagcc aaaatacaag gtgctatttg gcgaacatac attgaaatat
     4621 gtcacccaaa tcaccgattc taagggtaac aatctatttc cacacttaaa tgccgagcaa
     4681 tttgcaaaaa tggtgctgga acgtagccat tatgctcctt tcagccttga ttttgccggc
     4741 gtggagttcg atttagtgat gatgaaacat ttgcttgaaa accctcacga aaaaggcatt
     4801 taccttaaat acagccccaa actgtatgaa gacacggaag agttttatac cgtctatttg
     4861 aattggctca aaaatgaagt cgggattgat ttagtgcaag ataagattgc aatcaagccg
     4921 tttgctcata gctgtaacgg cggcattcaa attaatcaat ggggcgaaag tgcagtaaaa
     4981 gggctatttg cgattggtga gatttctcac tgcattgaag gggcaaaccg tatgggtggt
     5041 aactcagtcg gcggtggctt agtgtttgcc aaacgtgcgg tatcggaaat tgttcgttcg
     5101 ctagaacaga atgatttgca aaaaaacagc gaaatctcac cgcttgcaat ggcagaaaaa
     5161 gcattaaatc agcttgctaa ttcaactgct aatctagaac taactgccag tgaagtgctt
     5221 tctactattc gagagaaaat ggcgttatac gccaatgtgt accgcactcg gagcaacctc
     5281 aaactactac ttgaggaatt aaaccgtttg gaacaagaat ttgacccaat ctatcacgct
     5341 caatatcaag gcattgaaat ttacaatgcg ttaaaaaccg ctcaattagt ggtcaatcaa
     5401 atgctaaatg agcagagttt aggcgcgcac tacttagatg aacaaaatta gtctataata
     5461 gccaacttat ttaaaaataa taaattagga gtataaatga ataactacct acttaaatta
     5521 aaaccaatga cagcggtcat actttcgctt ttgacggtct ttatcttagg ttttacaatg
     5581 attgggcttg gctgggtgcc tcaaatttca attctagccg tgattgtaat gctgctactt
     5641 ttcggcttaa tcaataaaat tccgtttaaa aatatgcaag agggtatggc aagcgggctg
     5701 atgtccagta ttggtgcggt ttacctattc ttttttattg gattattggt ttcagctttg
     5761 atgatgtctg gtgcgattcc aaccttaatg tattatggct taagtgtgat ttcacctgaa
     5821 ttattttaca tttctgcatt cgcattaagt tctgtagtag gtattgcgtt aggcagtagt
     5881 ttaaccacaa ccgccaccat cggtgttgct tttatcggta tggcttctgc tttcggtgcg
     5941 aaccctgcga ttgtagcagg tgcggtggtt tcaggggcgt tttttggcga taaaacctct
     6001 ccactttcag acactactac cattgctgct tccgtagtgg gcattgatat gttcgctcat
     6061 atccgtaata tgctttatac caccatccct gcgtggattt taacagcatt attcctttgg
     6121 tttttaaccg aacctactga tgcacaaaat ttagcaaata tagagaacct aaaaaccaat
     6181 ttgcaagaaa caggattaat acacggctac tctttgctac cttttgttac gttaatttta
     6241 atggcaatta agcgtgttaa ttcaatttat attattgtaa ctacagtaac actctcgcta
     6301 attattacct atatccacag ttcgccaacc cttgaacagc tagggaatta tttctttacc
     6361 agctataaac ctgttgaagg cgtagattta ggcgaagtgg catcattagt ctctcgtggt
     6421 ggcattaata gtatgttctt taccatcact attgtattgc ttgcgttaag tttaggtggt
     6481 cttttacaaa tcctcggcat tttaccggca ctacttaacg gcattagcgg ctttttaacc
     6541 agcacaggac gtgttactct tgtagttgcc agcactgcgg tgggaattaa cgtcttaatc
     6601 ggtgagcaat atttgagctt attgttatca ggtaatactt tccgctcaac ttatgaacgt
     6661 ttaggcttag aagtgaaaaa cttatctcgc accattgaag atgcaggcac ggtaatcaac
     6721 ccattagtcc cttggggcgt gtgcggtgtg tttatcagta gcgtattaaa tgtaccggtg
     6781 ttagattatc taccatacgc attcttctgt tatatctgtt taatccttac cttaattttt
     6841 ggatttaccg gggtaacaat cagtaaaaaa gctaactaat atgtgaaaaa tgcctacaaa
     6901 tttgtaggca ttttcttttc attagagctg aagaacagct tgtttggcta actctctggc
     6961 gagtttatca atatgcaata catcagcaat ttcactgact tgaattggag caatattttc
     7021 caccaccgtt ctattcacgt tcacaatatc cgtaaagcgg atttgctcgt ttaaaaacgc
     7081 ctcaacggca atttcattgg cggcattcat cgctgttgtc gcatactgtc cttctgcaaa
     7141 agcctcaatc gctaatttta agttcgggta acgggcaaaa tccggctcga taaaggttag
     7201 ctcttttaat ttaaagaaat ctaatggtgg cacaccagca ttaattcggt ttggatatgc
     7261 catcgtgtga gcaatcggcg tacacatatc cgggttgccc atttgagcaa tcaccgagcc
     7321 atcaatataa cgcaccatcg aatgaataat tgattgtggg tgaatgatga tatccatttc
     7381 ctcggctgtt gcattaaaca accagcgggc ttcaatatac tccaagcctt tgttcatcat
     7441 tgtggctgaa tcgactgaaa ttttcttacc catagaccaa ttggggtgag caactgcttg
     7501 agccggtgta atcgcatcaa attcatttaa tggcttagta cggaacggtc cacccgatcc
     7561 tgttaaaata attttactga cacccagttc agcgagtgga caaaacccga ctttttgctg
     7621 tgcctccggt ggaagcgatt ggaaaatcgc attgtgttca ctatctaccg gtaagagctt
     7681 cgcaccggat ttacgagcct catcaataaa aatttgccca caggttacca gcgattcttt
     7741 atttgccaac aatacggttt tacccgcttt caccgcagaa agtgtcggta ataaccctgc
     7801 tgctcctaca atagcagcca ttaccatatc cacttcggaa tgtgcagcaa gctcacaaat
     7861 agctgcttga ccggctaaca cttcagtttt agaccccaaa gttgccaatg ccgtttttaa
     7921 tgcttgtgcg gatttttcat catccattgc cgcaaaagtc ggctgatatt gacgacattg
     7981 ctccagcatt aattcaacat tctttccacc aactaacgca aacacctcat attggtttgg
     8041 gtttgggtta tgctcaacta ccgataaagt gcttttaccg atagaacctg ttgaacctaa
     8101 aatcactaat tttttcattt tgtttctctt tatctaatga ctgataaggg cgaaagattt
     8161 ttcgccccta cgccgtaatt atgcggatac tcattcgttg tttgtaaatt gtttagcaaa
     8221 aacaaccgct tgtagattaa ctcgaaagcc gttttgcttc gataacatca tctaattgag
     8281 cgatacgagc caataattta ttcaaaattt caacgttgtt aagctgaatt tccatatcaa
     8341 tggtggcaag gctacgtttc acatcggttc ggctggaaac gcctaacacg tttacttttt
     8401 catttgccat aattgcactg acatctcgca gtaagccgtt gcggtcattg gcaatcactc
     8461 gaatggttaa gcttaaacca gaggtatagc cttcgcccca atccgcctcc acaactcgtt
     8521 ctgggttgag actttgtaat tcaaacagtt gctcacaatc tgcacggtga atcgaaatcc
     8581 ctctgccttg tgtgatgtaa cccacgattt catcacccgg aatcggctgg cagcaacggg
     8641 caatggaatg cattaagttc cctacgccct ctacaatcac atagcctttg cttggcttcg
     8701 gctgattggt gtttttattc acctgtttaa gtaccgcttc gtcttcttgt tcggcggtcg
     8761 gtttaattaa ttttgattgc aagaagtgca ttaactgatg aagacgaata tcgccaccgc
     8821 caataccggc atagagatca tcaaactgtt ttaagttata gcgaggcaga gcatattctt
     8881 caatttgctt gagcgtaaaa ccgaattttt ccatttcggc ttcaagggcc tctttgccga
     8941 ttggaatatt tttctctcgg tctaattttt taaaccacgc aataattttt gagcgagctt
     9001 tgcctgtatt cacaaaaccg gtattcggat tgagccaatc tcggcttggg ttcggctctt
     9061 tttgggtgat aatttcaacc tgatcgccca tttgcagatg ataagtaaac ggcacaatcc
     9121 gccctgccac ttttgctcca atgcaacgat gcccgatttc actatgaatt gcataagcaa
     9181 aatcaagtgg tgtagctttt tgcggtaaat cgatcacctc gcctcttggg gtaaagacat
     9241 aaacacgatc atcaaaaatt tgcgaacgca tttctgccac catatcgcct gaatcggcaa
     9301 gatcgttttg ccactcaagc aatttgcgta gccacacgat tttttcttcg tagcccgaac
     9361 gccctgcggt tgccccctct ttatatttcc agtgagcggc tacgcctagc tccgcatcat
     9421 cgtgcatttg ctgggtacga atttgcactt caatcgtttt ttcaccttcg cctaatacaa
     9481 ctgtatgaat agactgataa ccgttaggtt ttgggttagc aatataatca tcgaaatgtt
     9541 ccggcaaatg ggtatatttg gtgtgaataa tgcctaatgc cgtgtagcaa tcttctaacg
     9601 ttggcactat tacccgcaca gcccgaatat cgaaaagctg attaaatctc aaccgttttt
     9661 tctgcatttt cttccaaatg ctgtagatat gtttcgggcg tccataaatc tgcacattct
     9721 tgatttcagg acttaaacaa gcggtcagat ctgctacaaa atttgcaata taacgctcac
     9781 gatccacacg gcgttcgccc aattcaaatt tagcaatttg agtgtaagag acagggtgta
     9841 aaatgcggaa acagtaatct tccagctccc atttgagctg cccgataccc aatcgattgg
     9901 caagcggtgc ataaacgtga gaacattctt tagcggcgag aacggtatct tcttcggaat
     9961 ggtggttact atcacgcagg taggcaattc gttcggcaag tttaatcacc acacagcgga
    10021 aatcgtccac catcgccaac aacatgcgac ggatattgtc gatttgcaga tccgacacat
    10081 tattggcact aagctggcgg atattctcca tttcgagtac gcctttaact aagtttttga
    10141 tgtcgttact gaaatcttct tttaattgca ccaagtcgat cacattgtgt tttaccagag
    10201 gaaaaagcat tgcggcaact aagctgtcat cgtccatatt caagccgtgc aatacttcaa
    10261 ccatctcaat gccataccac atcagactat agtcggtatc cagtttttcc tgtgcataac
    10321 tccacgcatt gtgaatctgc tcaaaagtca caggagacat attcaggctt gcacaccaac
    10381 tcgacaactc aaaattattc ggttcaagtt gatgggaata acgaattgct accataaggc
    10441 ctccttgtaa atttttgtgg aaattagacc gcttgtacag cttaaaatgg agttttatta
    10501 tacgtggaaa ttcagaaaaa atttacccct aatcgaaaat ttaatccaag ttatcttatc
    10561 ctctttactc cttaagaggt agcaaccttg tgataaatca aacctatcaa cacaatcatt
    10621 tgcacgactt cacgctcttg attaaaatac ttgcgttttt attttgatct ttatttttgt
    10681 gggtagcaat gacaatttta gctttaggaa tcaaccataa aaccgcctct gtgggtttaa
    10741 gagagaaagt ggcgtttgta gatgacaaac gtaaactggc tcttgaacaa atccaaacga
    10801 gcggattggc tgaaagcgtg gtcattctct ctacctgcaa ccgcaccgag ctctacttcc
    10861 accagcctaa tatttctccc cgtgaagaga gcgaggaaaa tatccaatgg cgtgaacaat
    10921 gtttccgctg gtttgcccaa attcatcaac ttgatgaaag cgagttgcgt gaatgtttat
    10981 attttaaaca aaatttagaa gccgctaacc acttaatgaa agtggcaagc ggattagatt
    11041 cactaatttt aggcgaacca caaattttag ggcaggttaa acaagcctat caatatagcg
    11101 aagatttcta ccaacatcag caaaaagcga tgtccaccaa gctatcacgc ttgttccaac
    11161 gcacctttgc caccgcaaaa cgagtacgtt ccgaaaccga aatcggcagc caagcggtgt
    11221 cagttgctta tgcagcttgc ggtttagccc gtcaaatttt cgataatttt ggcaagttac
    11281 gctttttgct ggtaggggca ggcgaaacca ttgaattagt cgcccgctat ttaattcaac
    11341 acggggcgaa aaatataatg ttggcaaacc gcacccatat tcgggcggaa atgttggcgg
    11401 aaaaactaaa cgctccaatg caaattctgt cgttaagtgc tttgcaactc ggtttaaacc
    11461 aagctgatgt agttattagc tccaccggta gccctgatac cctaatcagt aaagaaatgg
    11521 tggaaattgc gcaaaaacaa cgccaatttg acccactttt gctgattgat attgccgttc
    11581 ctcgtgatat tgacgaaaaa gccggcgact tagagggcgt ttacgcttat agtgttgatg
    11641 atttacaaaa tattatccaa cgcaatatgg ctcagcgtga agaagccgcc aaacaagccg
    11701 ctgaaatcgt caaagaggaa agcaaagcat tttttgaatg gttaaaacaa cagcagtcta
    11761 gtgatttaat caaacgctat cgtcaagatg cggagcaaac ccgccaagaa ttgttggaga
    11821 aagccctaca agcaatcagt caagggcaag acagccaaaa agtattaagt gagctaagct
    11881 acaaactcac caaccagtta ctacacgccc caacccaagc gttacaagca atggcaaaaa
    11941 gtggcaactc caccggctta caaagttttt caagagcatt acggctagaa gaatgatgct
    12001 cagcacattc ttttaggcat atttgcaaaa aattgaacat ttttaaccgc ttgcggacgc
    12061 acgccgtgcg tccctccccg tgttcccgat cttcttctaa tttcaacttc cccaaatttc
    12121 cgctataata agccaatttt tgcaactatt ttgcaaataa ccggatatag aggatacaaa
    12181 atgactcctg ttgttgccct agtgggcaga cctaatgtcg gcaaatcgac tttatttaac
    12241 cgtttaaccc gtactcgaga tgccttagta gcagatttcc ccggcttaac ccgagaccgt
    12301 aaatatggtc acgctaatat tgccggctac gatttcattg taatcgacac cggtggtatt
    12361 gatggtacag aggaaggcgt ggaagaaaaa atggcggaac aatctttatt ggcgattgaa
    12421 gaagccgatg tggtgctttt cttggttgat gcccgagccg gattattacc tgccgatgtc
    12481 ggcattgctc aatatttacg ccaacgtgaa aaaaccacgg tggtggttgc caataaaacc
    12541 gatggtattg atgccgactc tcactgtgcc gaattctatc aattaggctt aggcgaagta
    12601 gaacaaattg ctgcagcaca agggcgtggg gttacgcagc ttatcgagca agtgttagct
    12661 cctttaggcg aacagctaaa tgccgagcaa tttaaccaag aacaagcggg cgaaaatgct
    12721 aaaaatgttg caaatgaaga aacagacgaa tgggataccg aattcgactt tgagaacgaa
    12781 gaagatgcgg cattattaga tgaagccttg gcagaagaag cagaagagtc gattgaagat
    12841 aaaaatatca aaattgcgat tgtcggtcgt ccgaatgttg gtaaatcaac actgaccaac
    12901 cgcattttag gcgaagagcg tgtggtagtt tatgatatgc caggaacaac ccgtgattct
    12961 atttatattc caatggagcg tgacggtcag caatatacca tcattgatac cgccggtgtg
    13021 cgtaaacgtg gcaaagtgaa tttagcggtt gagaaattct cggtaattaa aaccttgcaa
    13081 gccattcaag atgcaaacgt ggtgctatta actattgagg cccgagaagg tatttccgat
    13141 caggatttat cactactcgg ctttattcta aatgctggtc gttcattggt gattgtggta
    13201 aacaaatggg acggcttgtc gcaagatatt aaagatcaag tgaaatcaga gttagatcgc
    13261 cgtttagatt ttattgattt tgccagagtg cattttatct cggcattgca cggctcaggc
    13321 gtggggaatt tgtttgattc agtaaaagaa gcctacgctt gtgcaacgca aaaaacctcg
    13381 acctcaatgc tgactcgcat tttgcgtatg gctgctgatg aacaccaacc accattggtt
    13441 aatggtcgcc gagtgaaatt aaaatatgct cacccgggtg gctacaaccc accaattatt
    13501 gtgattcacg gcaatcaggt tgaaaaatta gcggattctt acaaacgtta tttaagtaat
    13561 tatttccgta aaagtttaaa aattatcggc tcgccaattc ggattttatt cc
//
LOCUS       DANLRK010000063        13595 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.063, whole genome shotgun sequence.
ACCESSION   DANLRK010000063 DANLRK010000000
VERSION     DANLRK010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 13595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13595
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.063"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(140..1471)
                     /gene="pcnB"
                     /locus_tag="PXA33_001272"
     CDS             complement(140..1471)
                     /gene="pcnB"
                     /locus_tag="PXA33_001272"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HDL5120602.1"
                     /translation="MLHKKFAVNASQYRISPKDFPKNAISIVDKLQIAGYEAYIVGGC
                     IRDLLLEKKPKDFDIATNARPEEIQRIFGRQCRLIGRRFRLAHIVYGREIFEVATFRA
                     GHESHRNDKISKVSDEGMLLRDNVYGTLREDAERRDFTVNALYYDIKRNLIFDFFNGI
                     EDLRAGKLSLIGEPTIRYQEDPVRMLRAVRFMAKLDMFLDKATASPIRDMAVLLTNIP
                     AARLFDESLKLLQTGAGLKTYQLLREYELWQFLFPVLPAFFTAKGDSYAERMITKALN
                     STDERIADKLPVNPAFLFAALLWYPLREKMEQLKNEGGFNSYDAMMLAANDVLAESCN
                     AIALHRRHTSVIRDIWALQFQMMKRSGNRPQQTLEHVKFRAGFDLLVMRAEIEGGDLV
                     ELSAWWHEYQFSNEGQRTELLKSVRHLGNVPSEKKKTRRRRTFKKKPKVNG"
     gene            complement(1716..2162)
                     /gene="dksA"
                     /locus_tag="PXA33_001273"
     CDS             complement(1716..2162)
                     /gene="dksA"
                     /locus_tag="PXA33_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HDL5120603.1"
                     /translation="MTQAAKTSLGLLALAGVEPYELKKDEEYMNEAQREHFTKILKAW
                     HSQIMEEAERTKSQLQEDMTNFADPNDRASQEEEFSLELRNRDRERKLLKKIEQTLYK
                     IQEDEYGYCETCGVEIGIKRLEARPTADLCIDCKTLAEIREKQLGL"
     gene            complement(2412..3365)
                     /gene="thiB"
                     /locus_tag="PXA33_001274"
     CDS             complement(2412..3365)
                     /gene="thiB"
                     /locus_tag="PXA33_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HDL5120604.1"
                     /translation="MKFILSSLALLSASVYANQPTLTVYTYSSFASEYGPAPKLEQNF
                     EKECGCDVKFVPLDDSVTMFNRLRLEGAKTKADVVVGIDNFLQPEAEKSGLFAEFEAK
                     TKLETQQKIFFPYDTGSYAFIYDKEKLKNPPKSLKELVERQDLKVIYQDPRTSSVGRG
                     FLMWVNQVYGDEAPTAWKTLAKHTVTVGKGWSETYGAFLKGESDLVLSYTTSPLYHKW
                     HENTDKYVAAPFEEGHLVQVENAAIVKSSQQKALANQFLEFLHKPESQHIISYHNVMN
                     PVLTDGVDPAFAAMPTYKTIEFTPPTTETIQKWLADWSKSW"
     regulatory      complement(3409..3552)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3836..4822
                     /locus_tag="PXA33_001275"
     CDS             3836..4822
                     /locus_tag="PXA33_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDL5120605.1"
                     /translation="MSQLIRTLKGHIRDEIIKKGGWVNAHAHADRAFTMTPEKITVYQ
                     NANLQQKWDLVDEVKRNSSVEDYYRRFSQAIELMISQGVTAFGTFVDIDQVCEDRAII
                     AAHKAREVYKNDIILKFANQTLKGVIEPNAKKWFDIGSEMVDMIGGLPYRDELDYGKG
                     LEAMDILMDKAKSLGKMLHVHVDQFNSPKEKETEQLCDKSIEHGMQGRVVAIHGISIG
                     SHPKEYRKMLYQKMRDSQMMVIACPMAWIDSGRKEDLQPFHNALTPADELIPEGITVA
                     IGTDNICDYMVPLCEGDMWQELSLLSAGCRFTNLEEMANIASINGRKVLGLV"
     gene            complement(4876..5295)
                     /locus_tag="PXA33_001276"
     CDS             complement(4876..5295)
                     /locus_tag="PXA33_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HDL5120606.1"
                     /translation="MLGNILGSLASSVLSGNNQSTATQLIQNLLASQGGIEGLIAKFQ
                     QGGLDDVLRTWISSDEDNAPVSGEQISNVFGQENIQNVAQEAGVDAADASDLLAQFLP
                     KIVDTLTPNGNVEDLKNFDANDLLAQAAKGILGNLFK"
     gene            complement(5373..6128)
                     /gene="kdsB"
                     /locus_tag="PXA33_001277"
     CDS             complement(5373..6128)
                     /gene="kdsB"
                     /locus_tag="PXA33_001277"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HDL5120607.1"
                     /translation="MKFTVIIPARYASSRLPRKPLLDIAGKPMIQHVWEKAQLAGASR
                     VIIATDHPEIEALAQSFGAEVCMTSEKHNSGTERLAEVIEKMQIADSEIIVNIQGDEP
                     LIPPVIVTQVAQNLDAYQVNMATLGVKLATREELFNPNAVKVVADKNGMALYFSRASI
                     PFARDSFPDCDDSFVAAQGYLRHIGIYAYRAGFVKQYVAWQPTQLEQLESLEQLRALW
                     HGEKIHIEPAKEAPQVGVDTPEDLERVRAILAR"
     gene            complement(6138..7091)
                     /gene="trxB"
                     /locus_tag="PXA33_001278"
     CDS             complement(6138..7091)
                     /gene="trxB"
                     /locus_tag="PXA33_001278"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HDL5120608.1"
                     /translation="MTTKHTKLLILGSGPAGYTAAVYAARANLNPVLVTGMQQGGQLT
                     TTDEIENWPGEFEQITGTELMNKMLKHAEKFETEIVFDHIHSVDLSKRPFTLNGDLYT
                     FTCDALIIATGASAKYLGLPSEEHFKGKGVSACATCDGFFYRNKPVAVVGGGNTAVEE
                     ALYLANLASEVHLIHRRDSFRAEKILLGRLQKRIEEGKIILHTDRTVDEVLGDNMGVT
                     GLRLKSTKEELSEEIKVDGFFVAIGHAPNTAMFEGQLELENGYIKVKSGLEGNATATS
                     VEGVFAAGDVMDHNYRQAITSAGTGCMAALDAERFLDAQEA"
     gene            7258..8328
                     /gene="dhaK"
                     /locus_tag="PXA33_001279"
     CDS             7258..8328
                     /gene="dhaK"
                     /locus_tag="PXA33_001279"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012073177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HDL5120609.1"
                     /translation="MKKLINSIETVLDEQLSGFVKAHSDLTLNTEPVYIYRTTPSNQP
                     KVALISGGGSGHEPMHAGFIGKGMLDGACPGAIFTSPTPDQMFECGLAVDRGEGVLLI
                     IKNYTGDVLNFETATELLADSGVKVATVLVDDDVAVKDSLYTAGRRGVANTVLIEKLL
                     GAAADKGYNLSELAKLGHQLNNNGHSIGIALSACTVPAAGKPSFTLAENEMEFGVGIH
                     GEPGIERRPFENLDSTVKQMFETLIAHGDYQRKIRHWNTQTQQWDEIEESKVALKKGD
                     RVIALVNNLGAVPLSELYAVYDALEKECQTFGLSIERSLVGSYCTSLDMQGISITLLK
                     VDEDILKLWDAPVNTPAMRWGE"
     gene            8349..8975
                     /gene="dhaL"
                     /locus_tag="PXA33_001280"
     CDS             8349..8975
                     /gene="dhaL"
                     /locus_tag="PXA33_001280"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HDL5120610.1"
                     /translation="MTISKQQILKWLENCQQVMTEKRDYLTELDTVIGDGDHGLNMQR
                     GFSKALEKAHTVQDKDIGTLLKTVGMTLLSQVGGASGPLYGSFFIKAAQGATGKEQLS
                     FNELLALLQQGAEAIVQRGHAQVGDKTMCDLWLPLLEDLAQADSTQPIAQILLQAVEK
                     AENFANQTAPLMAKKGRASYLGERSIGHKDPGATSSYYIFKALAEALQ"
     gene            8985..9392
                     /gene="dhaM"
                     /locus_tag="PXA33_001281"
     CDS             8985..9392
                     /gene="dhaM"
                     /locus_tag="PXA33_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HDL5120611.1"
                     /translation="MVNLVIVSHSAKLAEGVGEILAQMAQGGCKIALAGGIEDTSNPI
                     GTDAVKIMQAIESVFSPDGVIVFVDLGSAILSTQTAIDLLEPELAEKVIISYAPLVEG
                     AFAAVVAASAGDSLEAVLNEANEAAALKQEQAN"
     gene            complement(9432..10277)
                     /locus_tag="PXA33_001282"
     CDS             complement(9432..10277)
                     /locus_tag="PXA33_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone YbbN"
                     /protein_id="HDL5120612.1"
                     /translation="MDYFVNVNEQNFSEVWAAAAQVPTVFHFISPREVASLEMDALLR
                     RISDENPNQFLLATVNCDEQQALAAQFRIQQIPTLYLFAGGQPIDAIQGAVSEQELRI
                     RLANILPKEEELKFQQALELLQDQRYTEALPLLKSAWEMTDKKNSDFALLYAETYIAM
                     KSVEEAREILKAIPIQDRDSRWHGLQAQIELLEQASESPELQQLQADYANSKTPEIAI
                     KLANQLHQAHKNEEALTLLFDWLKSDLNVANGEVKSQFLAILSALGTDPIVPKFRRQL
                     YSLLY"
     gene            complement(10321..10647)
                     /gene="glpE"
                     /locus_tag="PXA33_001283"
     CDS             complement(10321..10647)
                     /gene="glpE"
                     /locus_tag="PXA33_001283"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HDL5120613.1"
                     /translation="METTFKEISPQQAWELIENEGAVLADVRDARRYVYSHPQDAFHL
                     TNQSYGKFLDEVDYDEPVVVICYHGVSSQSTAQFLIEQGFENVYSVKGGFEAWERNGL
                     PIETSY"
     gene            10798..11256
                     /gene="exbB"
                     /locus_tag="PXA33_001284"
     CDS             10798..11256
                     /gene="exbB"
                     /locus_tag="PXA33_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-system energizer ExbB"
                     /protein_id="HDL5120614.1"
                     /translation="MEQLFQFLEHHTDFVILGLLGLLSVICLWKAIERIMFYSKVDIS
                     KYDTLEELDIDLTHNLTTISTIGSNAPYIGLLGTVVGILLTFYHLGQSGGDIDAASIM
                     VNLSLALKATAVGILVAIIAMVFYNGFGRKVEENKLKWLALQSKKPHQAV"
     gene            11293..11730
                     /gene="exbD"
                     /locus_tag="PXA33_001285"
     CDS             11293..11730
                     /gene="exbD"
                     /locus_tag="PXA33_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HDL5120615.1"
                     /translation="MKKFDEINIIPFIDIMLVLLAIVLVTASFISQGKIQVNVPKAST
                     TQPMKADDLAKLLTITENNEFFFNDQAITKEQLIAEVATWDKSQKVSLKVDGAVAFEK
                     FVELTDILSANEIKNVAIITKKETAPAPSSTPGSPAQVPAVAP"
     gene            11746..12657
                     /locus_tag="PXA33_001286"
     CDS             11746..12657
                     /locus_tag="PXA33_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HDL5120616.1"
                     /translation="MKKKHSRIGLLASLAIHTALIGGTFAWLHNHKVEKIAEQNSLSM
                     EMVAALLEQPQVAVAPDPITEEVKEIEPEKAEVPPEPKLTPEPTPEPEAIPDPTLNPK
                     KEKPKEEKRKERHHHKHKHRHKEKREELQEQEKPKDKPKERPKHHHKHIKALERGPEI
                     KQGIVAKAIPNAAEGEKVVAGVVGGQKNGSPNSTSTTGSANTGASSGNASELAAYKVA
                     LQRALQRKANNSYPQREKMMRKTGTVTIKFTITSSGSITNVSVLNSSGNSNLDNAAVR
                     SAEGLSMPPPPAGFPPTVTVPVRFALE"
     gene            complement(12726..>13595)
                     /gene="cysZ"
                     /locus_tag="PXA33_001287"
     CDS             complement(12726..>13595)
                     /gene="cysZ"
                     /locus_tag="PXA33_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HDL5120617.1"
                     /translation="FSEPQNQVGLGFHYFIYGWRLLLQRQFLPFVIFPILINTVLMVG
                     LIWGFFANIGGLLDMLLPSWLEWLSFVLIPLIFLMILVGFYFAFTTLANFVAAPFNAL
                     LAEKVELQLTGEPLGDMSFGAMLKDVPRMLKREWQKMMYSIPRLIALFVLGFLPVVGQ
                     TVAPVLTFIFGAWLIAIQYCDYPFDNHKISFQRMRNALSQSRTLNFTFGALVSLFTMI
                     PFVNLVVMPVAVCGATALWVKEYRNFFLNNQTGEFSKADYTFTKVNSTSLSTETRTGE
                     VSPNVRNGDIRKQ"
BASE COUNT         4071 a         2934 c         2725 g         3865 t
ORIGIN      
        1 cgttcagcga aataagcaga gagaggggac aagcggtaac ttttattaaa ttttttacac
       61 atttcccctc tccctgattt ttcttctgaa cgaagaaaaa tcgctccctc tcccacaagg
      121 ggagagggaa atatattaat tatccattta ctttcggttt tttcttaaag gttctacggc
      181 ggcgtgtttt ctttttctcg ctaggcacat tgcctaaatg acgaaccgat ttcagcaatt
      241 cagtacgctg accttcattg ctaaattggt attcgtgcca ccaagcggaa agttcgacca
      301 aatcgccgcc ttcaatttct gcacgcatca ctaataaatc aaaccctgca cggaatttga
      361 cgtgttcaag ggtttgttgc ggtcggttgc cacttcgttt catcatctga aattgcaatg
      421 cccaaatatc tcgaatgact gaagtgtgtc tgcggtgcaa cgcaatcgcg ttgcaacttt
      481 ccgccagcac atcatttgct gccagcatca ttgcatcgta gctgttgaag ccaccttcgt
      541 ttttaagctg ctccattttc tcccggagcg gataccaaag tagtgcagca aataaaaacg
      601 caggatttac cggtaattta tcggcaattc gctcatcagt tgaatttaac gctttggtaa
      661 tcattctttc ggcataggaa tcacctttgg cagtaaaaaa tgccggcaat accggaaaca
      721 agaattgcca tagttcatat tcacgcaaaa gctgataagt tttcagccct gcaccggttt
      781 gtaataattt gagtgattcg tcaaacaaac gggctgccgg aatgttggtt aacaacaccg
      841 ccatatcccg aattggggag gccgtggctt tatccaagaa catatccaac ttcgccataa
      901 aacgaacggc tcgcaacatt ctcaccggat cttcttggta acgaatagta ggctctccga
      961 taagcgaaag tttacctgct cgtaagtctt caataccgtt gaagaaatca aaaataagat
     1021 tgcgttttat atcgtaatat aaggcgttta cggtgaaatc acggcgctct gcatcttcac
     1081 ggagcgtgcc gtaaacatta tcacgcagta gcattccttc atcgctgact ttagaaattt
     1141 tgtcattgcg atgagattcg tgtcctgcac ggaaagtcgc cacttcaaaa atttcacgcc
     1201 cgtaaacaat gtgtgccaaa cggaaacgac gtccgattaa tcggcattgc ctaccaaaaa
     1261 tacgctgaat ttcttcaggt ctcgcattag tcgcaatatc gaaatcttta ggctttttct
     1321 ccaagagtag atctcgaata cagccaccca caatataagc ttcataaccg gcaatctgca
     1381 atttatcgac aatcgaaatc gcatttttag gaaaatcctt cgggctaatt ctatattggc
     1441 tggcattcac cgcaaatttc ttgtgtaaca aatgggaggg aggattatct tgttgtttct
     1501 tcccacctct ataagtcttt tggtccgtta agttcggggc tgggcgttta gaacgggaag
     1561 ttttgggttc tctttccgct cttttttcac tctctacctt ttcatttttc tgagccgact
     1621 ttttaggctt ggaaaataat gatttaatat aattaaaaat aatacacctc taaatagtaa
     1681 atgcctctta aaaaagaggc accctgtaaa gttgattata agcctagctg cttttctcga
     1741 atttcagcta aagttttaca gtcaatacat aaatcagctg ttgggcgtgc ttctaaacgt
     1801 ttaatcccaa tttctacgcc acaggtttcg caataaccat attcatcttc ttggatttta
     1861 tacaaggttt gctcaatttt tttcaataat ttgcgttcac gatcacggtt acgaagctct
     1921 aaactgaact cttcttcttg actggctcgg tcgttcggat cggcaaagtt agtcatatct
     1981 tcttgaagct ggcttttggt acgctccgct tcttccataa tttgactgtg ccacgctttc
     2041 agaattttgg tgaaatgctc acgttgagct tcattcatat attcttcgtc ttttttaagc
     2101 tcataaggct caacgcctgc taatgctaat aaaccgagtg aggttttagc cgcttgagtc
     2161 atagatattt actccttgtt ccctgtttat tggaagtttt attgttaaaa ttattgcccg
     2221 aatacagact aacttccttg taaccgcact atacgaaaaa atcaacatct tcgggtttta
     2281 aataatggcg gctataaata acacaaaaac gactcattaa tcaaatgaat ttgcaaaaat
     2341 tttgcattca aattgatttg attgcacaaa aaaatagccg aacatccatt cggctatttt
     2401 tcaaacactt tttaccagct ctttgaccaa tcggctaacc atttttggat agtttcagta
     2461 gtcggtggcg taaattcaat ggttttatag gttggcattg ccgcaaaagc cggatcaacg
     2521 ccgtcggtta aaaccggatt cattacgttg tggtaagaaa taatatgttg cgattccggt
     2581 ttgtgcaaaa attcaagaaa ttgatttgcc aatgcttttt gttggctgct tttgacaata
     2641 gccgcatttt ctacttgcac caaatgccct tcctcaaacg gagcagccac atatttatcg
     2701 gtgttttcgt gccacttatg atataaaggc gatgtagtat agcttagtac caaatccgac
     2761 tcgcctttta agaacgctcc ataagtttca gaccaacctt tgcctaccgt taccgtatgt
     2821 tttgccaatg ttttccaagc agttggagct tcatcgccat acacttgatt tacccacatt
     2881 aagaagcccc gcccaacgct gctggtacgt gggtcttggt aaatcacttt taaatcttgg
     2941 cgttccacca actcttttaa actttttggt ggatttttca gcttctcttt atcgtaaata
     3001 aaagcataag aacccgtgtc ataagggaag aaaattttct gttgggtttc taatttggtc
     3061 tttgcttcaa actcggcaaa caagccactt ttttcggctt caggttgcag gaaattatca
     3121 ataccgacca ccacatcagc tttggttttt gctccctcca aacgcaagcg gttgaacatc
     3181 gttacgctat cgtcaagcgg aacaaatttg acatcacagc cacactcttt ctcaaaattc
     3241 tgctctaatt taggagcagg tccatattcc gaagcaaacg agctgtaagt gtaaacggtt
     3301 aaggtcggtt gattggcata aacacttgca cttaataacg caagtgaaga gaggataaat
     3361 ttcataacaa ttccttctaa attaaatgaa tagaaggatt taagaaaggc aagttgctat
     3421 ctcaaatccc tacgctgata ttaatcagat caggttctat gggtatttct cagccacaag
     3481 cggttaaatt tcggcaaaaa tttgcaaatt tttacgcaaa aacaaccgct tgttgcaccc
     3541 caatgagaac ggcttcattg taaaaagttt gcaggcagat ttcaaccaaa gcaaacgttt
     3601 gctatttgta ttaaaaatcc gtatactacc gccctagtcc aaacaaggaa ctagaaatga
     3661 attgcacaac atcggttaaa tcaaattccc aacagctacg cttcaacttt agaggcgtag
     3721 ctgtttttct attcatttca aaccattcaa aatcgctgtt atagcgattt tttttcgcac
     3781 gcttgcaatt accgcaagcg gttgagccac attaacagag agaaaaggaa taaaaatgag
     3841 ccaattgatt cgtaccctaa aaggacatat tcgtgatgaa atcatcaaaa aaggcggttg
     3901 ggtaaatgca cacgcccacg cagatcgtgc atttacgatg acgcctgaaa aaatcaccgt
     3961 ttaccaaaat gcgaatctac agcaaaaatg ggatttggtc gatgaggtaa aacgtaattc
     4021 aagcgtagaa gattactacc gccgcttttc ccaagcgatt gaattgatga tttcacaagg
     4081 ggtaacggca tttggtactt ttgtggatat cgaccaagta tgtgaagaca gagccattat
     4141 tgccgctcac aaagcccgtg aagtatataa aaacgacatc atcttgaaat ttgctaacca
     4201 aacgttgaaa ggcgtgattg aacccaatgc gaaaaagtgg ttcgatatcg gctcggaaat
     4261 ggtggatatg attggtggct tgccttaccg tgacgaactg gattacggca aaggcttgga
     4321 agcaatggat attttaatgg ataaagccaa atcgctcggc aaaatgctcc acgtccacgt
     4381 tgatcaattt aactcaccta aagaaaaaga gaccgaacaa ctgtgtgata aatcgattga
     4441 gcatggtatg caagggcgag tggttgccat tcacgggatt tccatcggct cgcacccgaa
     4501 agaatatcga aaaatgcttt accaaaaaat gcgtgatagc caaatgatgg tcattgcctg
     4561 tccgatggcg tggattgata gcggacgtaa ggaagattta caaccgttcc ataacgcttt
     4621 aacccctgct gatgaactca ttcctgaagg cattactgtt gctatcggca cagataatat
     4681 ctgtgattat atggtaccgc tttgcgaggg cgatatgtgg caagaactca gccttttatc
     4741 agcaggctgt cgttttacta acctagaaga aatggcaaat attgccagta tcaatggtcg
     4801 aaaagtactg ggtttagtct aactgaataa acgaaaggcg aaccctaggt tcgcctttaa
     4861 taacagcata accgattatt taaacaagtt acccaaaatc ccttttgccg cttgagctaa
     4921 taagtcattt gcatcaaagt tttttaagtc ttcaacatta ccattcgggg ttaaagtatc
     4981 cacgattttt ggcaagaact gggctaataa atcgctcgca tcagcagcat caacacccgc
     5041 ttcttgagcc acattttgaa tgttttcttg cccgaacaca ttactgattt gttcgcccga
     5101 aacaggtgca ttgtcttcat cgctgctgat ccaagtgcgt aacacatcat ctaatccgcc
     5161 ttgttggaat ttggcaatca gcccttcaat cccaccttgt gaggcgagta aattttgaat
     5221 taattgagtt gcagttgatt ggttattgcc actcaatact gaagaagcca atgaacctaa
     5281 aatatttcct aacataattt ctcctatgca agcggtacaa ttttgcgaaa attttgcaaa
     5341 aaaacagcaa acttcgaccg cttgtaatca ctttaacgag ctaaaatggc tcgcactctt
     5401 tccaaatctt ccggcgtatc cacccccact tgcggggctt ctttcgccgg ttcgatatga
     5461 attttttcac cgtgccataa ggctcttagt tgctcaaggg actcaagctg ttctaattgt
     5521 gtcggctgcc acgccacata ttgtttcaca aaaccggctc ggtaagcgta aatgccgata
     5581 tggcgtaaat aaccttgagc ggcaacaaaa ctgtcatcac aatccggaaa actgtctctg
     5641 gcaaacggaa tgctggctcg ggaaaaataa agtgccattc cgtttttgtc tgccaccact
     5701 ttcaccgcat ttgggttaaa tagctcttct cgggtcgcta atttcacacc gagtgttgcc
     5761 atattgactt gataagcatc taaattttga gctacctgag taacaattac cggtggaatc
     5821 agcggttcat caccctgaat attcacaata atttcgctgt cggcaatctg cattttttcg
     5881 atcacttccg ccaaacgctc agtgccggaa ttgtgttttt ccgaagtcat acagacttcc
     5941 gccccaaaag attgagcgag ggcttcaatt tcagggtgat cggtggcaat aatcacacgg
     6001 cttgcaccgg caagctgagc tttttcccaa acgtgttgaa tcatcggttt gcctgcaata
     6061 tccaacaacg gcttacgagg caaacggctg gaagcgtaac gagcaggaat aattacagtg
     6121 aatttcatag gctatcctta tgcttcttga gcatctaaga aacgttctgc atccagggct
     6181 gccatacaac ctgtgccggc agaggtaatc gcttggcgat agttatgatc catcacatca
     6241 ccggcagcaa atacgccttc cacagaagtg gcagtagcat tgccttccaa acctgatttc
     6301 actttaatgt agccgttttc aagttctaat tgaccttcaa acattgccgt attcggtgcg
     6361 tgcccaatcg ccacaaagaa accatcaacc ttaatttctt cgctcaattc ttctttggtt
     6421 gattttaaac gtaagccggt aacgcccata ttatcgccta acacctcatc aaccgtacgg
     6481 tctgtgtgca gaataatttt gccttcttca atacgttttt gcagacgacc aagcaagatt
     6541 ttttcagcac ggaagctatc acggcggtga attaaatgca cttcacttgc caaatttgcc
     6601 aaataaagtg cttcttccac agcagtattc ccaccaccga ctaccgcaac cggtttattg
     6661 cgatagaaga aaccatcaca agttgcacaa gccgacacgc ctttgccttt gaaatgctct
     6721 tcagaaggca aacctaaata tttcgccgac gcaccggtgg caataattaa cgcatcacag
     6781 gtgaaagtgt ataaatctcc gtttagcgtg aatgggcgtt tagataaatc cactgaatga
     6841 atatgatcaa acacgatttc agtttcaaat ttttcagcgt gcttgagcat tttgttcatt
     6901 aactctgtgc cggtaatttg ttcaaattcc cccggccagt tctcgatttc atcggttgta
     6961 gtgagctgtc cgccttgttg cataccggtg actaaaaccg ggtttagatt ggcacgggcg
     7021 gcataaacag cagcggtata accggcaggc cctgagccta aaattaaaag tttggtgtgt
     7081 ttagtggtca taatgaatcc tctgaataaa tattaatgtt tattttagcc taaatttcag
     7141 ttcaactcta ttttcgtttt cgctcaactt ttttcaattt tgtgacaaag ttaacagaaa
     7201 tccgactgtg tttcttgtaa attgtgacag tctaattcat ttaaaagggg caaaaagatg
     7261 aaaaaattaa ttaattcaat agaaaccgtc ttagatgagc aactttctgg ttttgtgaaa
     7321 gcacattctg atttaacact taacacagaa cctgtctata tttaccgcac aacgccaagt
     7381 aatcaaccta aagtagccct tatttccggt ggtgggagtg ggcacgagcc aatgcacgcc
     7441 ggttttatcg gtaaaggaat gttagacgga gcttgccccg gagcaatctt tacatctcca
     7501 acgccggatc aaatgtttga atgcggttta gcggttgatc gtggcgaggg tgtattgcta
     7561 attattaaaa actataccgg tgatgtgctg aattttgaaa cagccaccga gctacttgca
     7621 gatagcggtg taaaagtcgc aaccgtgttg gttgatgatg atgtggcagt caaagacagt
     7681 ttatatactg ccggccgccg cggtgttgca aatacagtat taattgaaaa gctgttagga
     7741 gctgcagcgg ataaaggcta taacttatcc gaattagcca aattagggca tcagttaaac
     7801 aataacgggc attctatcgg aattgcctta tctgcttgta ctgtgccggc tgccggtaag
     7861 ccatcattta ctttagctga aaatgagatg gaatttgggg taggtattca cggagaaccg
     7921 ggcattgaac gtcgcccctt tgaaaatctg gatagtaccg tgaaacaaat gtttgagacc
     7981 ttaattgctc acggcgatta tcaacgtaaa attcgccatt ggaatacaca aacccaacaa
     8041 tgggacgaaa tcgaagaaag caaagtggca ttgaaaaaag gtgatcgtgt tattgcttta
     8101 gttaataact taggagcagt gccactatcc gaactttatg ctgtttatga tgcgttagaa
     8161 aaagagtgcc aaactttcgg cttaagcatt gaacgcagtc ttgttggatc atattgcacc
     8221 tcgctggaca tgcaaggtat ctctatcaca ttactgaaag ttgatgagga tattctcaaa
     8281 ctatgggatg ctccggtaaa tacaccggca atgcgttggg gtgaataatt agattgaaag
     8341 gataagttat gacaatatca aaacaacaga tcttaaaatg gcttgaaaat tgccaacagg
     8401 taatgactga aaaacgggat tatttaaccg aattggatac cgttattggc gatggcgatc
     8461 acggtttaaa tatgcagcgt ggatttagta aagcacttga aaaagcccat accgtgcaag
     8521 ataaagatat cggcaccctg cttaaaaccg ttggaatgac attattatcg caagtagggg
     8581 gagcgagcgg ccctctctat ggttcatttt ttattaaagc ggctcaaggt gctacaggca
     8641 aagagcaact gagctttaac gagttactgg cacttctaca gcaaggagca gaagctatcg
     8701 tacaacgtgg acacgcacaa gttggcgata aaacgatgtg tgatttatgg ctgccattac
     8761 tcgaagactt agctcaagcc gatagcactc aaccaattgc tcaaatctta ttacaagcgg
     8821 tcgaaaaagc agaaaatttt gcaaatcaaa ccgctccatt aatggcgaaa aaagggcgtg
     8881 ccagttactt gggcgaacgc agtatcgggc ataaagatcc gggagcaacg tccagctact
     8941 atatttttaa agccttagct gaagcattac agtaggaggg gtaaatggtt aatttagtga
     9001 ttgtttcaca tagtgctaaa ctggcggaag gcgtggggga aattttggct caaatggcac
     9061 aggggggctg taaaatcgcc cttgccggcg gcattgagga tacctcaaat cctatcggca
     9121 ctgatgcggt aaaaataatg caagcgattg aatcggtatt tagcccagac ggtgtgattg
     9181 tgtttgtcga tttaggcagt gcaattttaa gtacccaaac cgccatagat ttactggagc
     9241 cggaacttgc cgaaaaagtg atcattagct acgctccttt agttgaaggt gcttttgctg
     9301 ctgtagttgc agcctctgcc ggcgatagtt tagaagcggt attaaatgaa gcgaacgaag
     9361 ccgctgctct taagcaggaa caagccaatt agtaaaaatg ccgtatcgaa aaatacggca
     9421 ttttagtttt attagtacaa tagtgaataa agctgtcttc taaatttcgg cacaatcggg
     9481 tctgtaccca gtgcagataa gattgccaga aattggcttt ttacctcgcc gttcgctaca
     9541 tttaaatccg attttaacca atcaaaaagt aacgtgagtg cttcttcatt tttatgagct
     9601 tggtgtagct gatttgccaa tttaatcgca atttcagggg ttttgctgtt cgcataatcc
     9661 gcttgaagct gttgtaattc gggcgattcg ctggcttgtt ccaataattc gatttgggct
     9721 tgcaaaccgt gccaacggct gtctctgtct tgaatcggga tcgctttaag gatttcacga
     9781 gcctcttcca cgcttttcat tgcaatgtaa gtttcggcat agagcaaagc gaaatcgctg
     9841 tttttcttat cggtcatttc ccacgctgat ttcagcagcg gcaaggcttc ggtataacgc
     9901 tgatcttgca ataattccaa ggcttgttga aatttcagct cttcctcttt cggtaaaata
     9961 tttgccaacc gaatccgtaa ttcctgctca cttactgcac cttgaattgc atcaatcggc
    10021 tgaccgccgg caaacaaata gagcgttgga atttgttgaa tgcgaaattg agcagccaat
    10081 gcttgttgct catcacaatt taccgtcgcc aataaaaatt ggttagggtt ttcatccgaa
    10141 atacgacgta gtaaggcatc catttccaat gaagccactt cccttggcga aataaaatga
    10201 aacaccgtcg gcacttgtgc cgctgctgcc catacttcac taaaattctg ctcgtttaca
    10261 tttacaaagt agtccataca aaaccttctt aagctaaaaa ttacttttta tattgtagca
    10321 ttaataagaa gtttcaatag gtagcccgtt tctctcccac gcttcaaaac caccttttac
    10381 actataaaca ttctcaaacc cttgttcgat taaaaactgt gctgtacttt ggctactcac
    10441 tccgtgatag caaatcacaa ccaccggttc gtcataatcg acctcgtcta aaaacttacc
    10501 ataactctgg ttggttaaat gaaacgcatc ctgtgggtgg ctgtaaacat aacggcgggc
    10561 atcacgcaca tcggcaagca ccgcaccttc gttttcgatt aattcccaag cctgttgcgg
    10621 gctaatttct ttaaatgtag tttccataat cactttccct caaaaaacgt gcaactatac
    10681 cataaaattc taaaaagaat ggctattatt ccaagttgaa aatttgatgt ttgtcagata
    10741 taattgcaaa ctattttcaa atgcactgat ttaaaactac cacagtttga ggattttatg
    10801 gagcaattat ttcaattttt agaacatcat actgactttg ttatattagg attactaggc
    10861 ttattaagcg taatctgcct ttggaaagct attgaacgta ttatgtttta ttccaaagta
    10921 gatatttcaa aatacgatac cctcgaagaa ttagacatcg atttaaccca caatttaact
    10981 accatttcga ctatcggctc taatgcccct tatatcggct tattaggaac cgttgtcggt
    11041 attcttttaa ctttctatca tttaggtcaa tcaggtggtg atattgatgc ggcctctatt
    11101 atggtaaacc tttctttagc tctaaaagcc actgctgtcg gtatcttagt ggcaattatt
    11161 gcaatggttt tctacaatgg ttttggtcgt aaagtggaag aaaacaaact gaaatggttg
    11221 gcattacaat caaaaaagcc tcatcaagcg gtgtgattta gtccattttt tgcaaattaa
    11281 taaggcagaa caatgaaaaa attcgatgaa atcaacatca ttccatttat tgatattatg
    11341 ttggtattac tggctatcgt gttagttacc gcctctttca tttctcaggg taaaatccaa
    11401 gtaaatgtac cgaaagcaag tacaacacag ccaatgaaag cagacgattt agcgaagctt
    11461 ttaaccatta ccgaaaataa tgagtttttc tttaatgatc aagcaataac taaagagcaa
    11521 ttaattgctg aagttgccac ttgggataaa agccaaaaag tgtcactcaa agttgatggt
    11581 gcagtcgcct tcgaaaaatt tgtggaatta accgatatcc tttctgcaaa tgaaattaaa
    11641 aatgtagcaa tcattactaa aaaagaaact gctccagcac cttcaagcac acctggtagc
    11701 cctgcacaag tgccagcagt cgcaccttaa atagggtaaa caggtatgaa aaaaaaacat
    11761 tctcgtatcg gcttattggc atctttggct attcacactg ccttaattgg tggcactttt
    11821 gcttggttac ataaccacaa agtggaaaaa attgccgagc aaaactctct ttctatggaa
    11881 atggtggcag cattattaga gcaacctcag gttgccgttg ctccggatcc tattaccgaa
    11941 gaggtcaaag aaatcgaacc ggagaaggca gaagtaccac ctgaacctaa gcttactccg
    12001 gagccaactc cagagccgga ggcaattcca gatcctacgc taaatccgaa aaaggaaaaa
    12061 ccgaaagaag aaaagcgtaa agagcgacac caccataaac ataagcatcg tcacaaagag
    12121 aaaagggaag agctacaaga gcaagaaaaa ccgaaagata agcctaaaga gcgcccgaaa
    12181 catcatcaca agcatattaa agcattagag cgcgggccag agataaaaca aggtattgtc
    12241 gctaaagcga ttccaaatgc ggctgaaggt gaaaaagtgg ttgcgggggt tgttggaggg
    12301 caaaaaaacg gttcaccaaa ttcaacctca actacaggtt cagcaaatac tggggctagc
    12361 agtggtaatg ccagtgaact tgctgcttat aaagtcgcat tacagcgagc attacagcgt
    12421 aaggcgaata actcttaccc tcagcgtgaa aaaatgatgc ggaaaaccgg tacagttacg
    12481 attaaattta ccattacatc gtccggttca attacaaatg tatccgtact caattcatcg
    12541 ggaaatagta atttagataa cgctgcggta cgcagtgcag aaggtttgag tatgcctccg
    12601 ccaccggcag gcttccctcc tacggtaaca gtaccggtaa gatttgccct tgaataaaaa
    12661 cagccctcaa aagcaatgta cttttgaggg ctttttattg caaaaaattg actaaaaatg
    12721 accgcttact gcttgcgaat atcgccattt ctcacattcg gactcacttc acccgtacga
    12781 gtttctgtgc ttaacgaggt agaatttact ttcgtaaaag tataatccgc tttgctgaat
    12841 tcacctgttt gattatttaa gaagaaattg cggtactctt ttacccataa tgccgttgcg
    12901 ccacaaactg ccacaggcat aactactaaa ttcacaaatg gaatcatcgt gaataggctc
    12961 actaaagcac caaaggtaaa atttaaagtg cgagactgcg acaacgcatt tcgcatacgc
    13021 tgaaaactga ttttatggtt atcaaacgga tagtcacaat attgaatggc gatcaaccaa
    13081 gcaccgaaga taaaagtcag cacaggtgct acggtttgcc caaccacagg caaaaagcct
    13141 aatacaaaaa gagcaatcag ccttggaatg ctatacatca ttttttgcca ttctcgtttc
    13201 agcattcgag gcacatcttt taacattgca ccgaatgaca tatcgcccaa aggctcgccg
    13261 gtaagttgca gttcgacttt ttctgctaat aacgcattaa agggagcagc aacaaagtta
    13321 gccaaggtag taaaagcaaa ataaaatccc actaatatca tcaaaaaaat cagtggaatc
    13381 agtacaaagc taagccattc cagccagctt ggcagtaaca tatccaacaa gccaccgata
    13441 ttggcaaaaa atccccaaat cagccctacc atcagcacgg tattaataag aattgggaaa
    13501 atcacaaaag gcaaaaattg acgttgtaac aataaccgcc agccatatat aaaataatga
    13561 aagcctaatc cgacttgatt ttgcggctca gaaaa
//
LOCUS       DANLRK010000064        13525 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.064, whole genome shotgun sequence.
ACCESSION   DANLRK010000064 DANLRK010000000
VERSION     DANLRK010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 13525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13525
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.064"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            169..963
                     /locus_tag="PXA33_001288"
     CDS             169..963
                     /locus_tag="PXA33_001288"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HDL5120618.1"
                     /translation="MRLIPLETDHDVSVWAARYIVDRINAFQPTKEKPFVLGLPTGGT
                     PEKTYKELIKLYQAGEVSFKNVVTFNMDEYVGLPKEHPESYHSFMFKHFFDHVDIPLE
                     NINILNGMAEDVNAECERYEEKIRFYGKIHLFMGGVGVDGHIAFNEPASSLSSRTRIK
                     TLTEDTRIANSRFFNNDVSQVPKFALTVGVGTLLDAEEVMILVTGHNKALALQACVEG
                     AVNHLWTISCLQLHRRGIVVCDEPATQELKVKTVKYFKELEKNVAR"
     gene            1044..2186
                     /gene="nagA"
                     /locus_tag="PXA33_001289"
     CDS             1044..2186
                     /gene="nagA"
                     /locus_tag="PXA33_001289"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDL5120619.1"
                     /translation="MNHYALTNCVIYTGEQVLYQHALVVKEDKIDSIVLEENLPLDLQ
                     KIDLKGANLSAGFIDLQLNGCGGVMFNEEITTRTLEIMQETNLRSGTTSYLPTFITSP
                     DEGVKQAVAVMREYLSKHKNQALGLHLEGPYLSVAKKGVHRPEFIREISPEMLDFLCD
                     NGDVITKITIAAENPTADHIKRFNDAGIIVSIGHSNATFAQTKQRIAEGAAFATHLHN
                     AMSPVSNGREGGVVGAVLDSDIYTGIICDGLHVEWGNIRIAKKAKGDKLVIVSDAVAA
                     AGSDIESFIFVGKKVTVKDGKCYDEFGALGGAAITMIESIRNVVQHCDISLNEAIRMS
                     TLYPAKAIGVEETLGSLEAGKIANLAIFDNQFEVKATAVNGQLQWH"
     gene            2268..2582
                     /locus_tag="PXA33_001290"
     CDS             2268..2582
                     /locus_tag="PXA33_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2322 family protein"
                     /protein_id="HDL5120620.1"
                     /translation="MQFKDYLATFPTIDHLAGLNVINSGEVIQHIPAIEGKLGSLKLY
                     NALFAKFDGKLTASAAKQGLEWFAEHVEDAKNNVGKHPNIDLLFRVIEQDLSLSLEPI
                     VK"
     gene            complement(2661..3851)
                     /locus_tag="PXA33_001291"
     CDS             complement(2661..3851)
                     /locus_tag="PXA33_001291"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010944663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HDL5120621.1"
                     /translation="MTQAIESVLAELKRGVENIYSEEDLITKLKENRPLRIKLGADPT
                     APDIHLGHTVVLNKLRQFQQFGHEVYFLIGDFTGMVGDPSGKNSTRPPLSREDVLRNA
                     ETYKEQIFKILDPQKTKIVFNSEWLGKLGTEGMIRLASNYTVARMLEREDFKNRFTNQ
                     QPIAIHEFIYPLLQGHDSVELKADVELGGTDQTFNLLVGRELQKSAGQKPQVAMTLPL
                     LVGLDGEKKMSKSLGNYIGVTEAPSEMFGKIMSISDDLMWDWYNLLSFRPLDEIAQLK
                     ADVENGKNPRDVKILLAKEIIARFHDEAAADAAEQEFINRFQKGAIPDEVPEFTFEGE
                     IGLAALLKEAGLVPSTSEAIRSAQQGGVKIDGEKVEDVKQTAQKGTFVYQVGKRKFAR
                     ITVK"
     gene            complement(4214..5518)
                     /locus_tag="PXA33_001292"
     CDS             complement(4214..5518)
                     /locus_tag="PXA33_001292"
                     /EC_number="7.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HDL5120622.1"
                     /translation="MESIIALFKGMGLMHLELGQAIMIVISLVLLWLAIARKFEPLLL
                     LPIGFGGLMSNIPEAGMAMTALDNLLHSGSAEQLAIIAAKVNSTSLDLHSIKDAIALA
                     APSVQNELEVMAGDMGYTAGVLALFYKVAIGYGVAPLIIFMGVGAMTDFGPLLANPRM
                     LMLGAAAQFGIFATVLGALGLNWLGIIDFTLPQAAAIGIIGGADGPTAIYLASKLAPE
                     LLGAIAVAAYSYMALVPLIQPPIMKALTTEQERKIRMVQLRNVSNREKIIFPIVLLLL
                     VALLLPDAAPLLGMFCFGNLLRTSGVVERLNDTAQNALINIVTIFLGLSVGAKLVADK
                     FLQPQTLGILVLGVIAFGIGTASGVIMAKIMNRFSQHKINPLIGSAGVSAVPMAARVS
                     NKVGQEYDKQNFLLMHAMGPNVAGVIGSAIAAGVMLKYISTM"
     gene            complement(5528..7354)
                     /gene="oadA"
                     /locus_tag="PXA33_001293"
     CDS             complement(5528..7354)
                     /gene="oadA"
                     /locus_tag="PXA33_001293"
                     /EC_number="7.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-extruding oxaloacetate decarboxylase
                     subunit alpha"
                     /protein_id="HDL5120623.1"
                     /translation="MTTQPKKIAITDVVLRDAHQSLFATRLRLDDMLPIASELDNIGY
                     WSLETWGGATFDSCIRFLGEDPWVRLRELKKAMPKTPMQMLLRGQNLLGYRHYADDVV
                     DKFVERSVANGMSVFRVFDALNDPRNMQAALQAVKKHGGHAQGTLSYTTSPVHTLQTW
                     LDVTEQLLEIGVDSLVIKDMSGILTPMAAYELVSEIKKRYDVELHLHCHSTTGMAEMA
                     LLKAIEAGVDGVDTSISSMSGTYGHPATESIVATLQGTPYETGLSIPALEKISAYFRN
                     VRKKYAKFEGQLRGIDSRILVAQVPGGMLTNLESQLKQQNASDKLDLVLQEIPRVRED
                     LGNIPLVTPTSQIVGTQAVMNVLMGERYKTIAKETAGILKGEYGRTPAPVNKALQDRV
                     LEGAEPITDRPADHLPPELEKLVAEVTASAKEKGITLSENMIDDVLIVALFQQVGWKY
                     LENRNNPSAFEPVPSADEAKPAQASAASAPKAQTQSGSAIYTVELEGKAFVVKVSEGG
                     DITNIAPTPATPAPQAVDSAPKSANTLSANAEPVNAPMAGNILKVEVKEGQQVAEGDV
                     LLILEAMKMETQICAAKSGTIQGINVKQGDVVAVNDTLMSIA"
     gene            complement(7394..7648)
                     /locus_tag="PXA33_001294"
     CDS             complement(7394..7648)
                     /locus_tag="PXA33_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase subunit gamma"
                     /protein_id="HDL5120624.1"
                     /translation="MAPAELFAEGINLMVAGMGFVMLFLLVLIYAISFISKIINQYFP
                     EPVSIPASKTVPQSPPDDLERLRPVIAAAIAHHRRINGQH"
     gene            complement(7838..9025)
                     /locus_tag="PXA33_001295"
     CDS             complement(7838..9025)
                     /locus_tag="PXA33_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HDL5120625.1"
                     /translation="MSNNNRSALLGAAFLMATSAIGPGFLTQTATFTQTLLASFGFVI
                     LLSILLDIGAQLNIWRIVAVSEKKAQDIANAVLPGAGYFLALLIVMGGLAFNIGNVGG
                     AGLGLNILTGLSPEMGAVVSGAIAVGVFLFHEAGKVMDRFAQIMGFVMIALTIFVAVK
                     ANLPIDDAVVHTFVPEKLDVIAIVTLVGGTVGGYITFAGAHRLLDAGIKGKENLKEVS
                     KSSVSAILIASIMRVVLFLAVLGVVSMGVQLDPKNPAATPFAHVAGDVGLMIFGVVIW
                     AASITSVIGAAYTSVSFITSFSPFIEKHKNCFIVAFIVVSTAVLATIGRPAQVLVFVG
                     TLNGLILPISLGLILLAAHKTKIVGDYKHPVWMTVSGAIVVILMAVLSAMTLSKYISG
                     LMA"
     gene            complement(9300..10037)
                     /gene="pxpA"
                     /locus_tag="PXA33_001296"
     CDS             complement(9300..10037)
                     /gene="pxpA"
                     /locus_tag="PXA33_001296"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HDL5120626.1"
                     /translation="MNHVDLNVDLAEGCGNDHSLLQLVTSANVCCGLHAGDYNEMRKA
                     INWAKENSVRVGAHPSFPDRENFGRTNMQLPDDELKACLLYQLGAAKALCDAENMPMS
                     YVKPHGALYNMAAKDPHLATLIANTVKQFDPNLKLMGLSGSLMLSIAEQHGLETISEV
                     FADRHYLADGSLVPRTRDDAMVESDDEAISQVLQMVLDGTVPTVDGGNVAIKADSICL
                     HGDGEHAVVFAERIKKELQAKGILVKA"
     gene            complement(10024..10953)
                     /locus_tag="PXA33_001297"
     CDS             complement(10024..10953)
                     /locus_tag="PXA33_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HDL5120627.1"
                     /translation="MIYIEIIQGFAHIQDLGRYGYRHLGVSHGGAMDSLALQAGNLLL
                     KNANNAPAIELTLGGMRITFDCDTPFCLTGALVEAELDGTPVFSYYRYTANARQTLTI
                     KRIVLGNYAYLCVAGGFLVPQVLGSASTALKAQFGGFQGRMLKAGDNIATGRRDSRLS
                     LMSIEPIDFTSRIRATASSEYEAFTADSRERFWQQGWQLQNNSNRMGYRFAEKALELT
                     ASLEMLSYAAPSGTVQVPPDGQPIVLMADAQTTGGYPKIACIIQADLGRLAQNPFGST
                     VQFEQVSREQAVEIYQKDQNYLETIRRKANESR"
     gene            complement(10950..11597)
                     /gene="pxpB"
                     /locus_tag="PXA33_001298"
     CDS             complement(10950..11597)
                     /gene="pxpB"
                     /locus_tag="PXA33_001298"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HDL5120628.1"
                     /translation="MLTIHHTSEHSLLCLAEPPAELAKQQQIWRVAEQARALDGVLEV
                     VVGMNNFTLFHAIGTDSTRLIQAVQQLWENDQAQAAQSPRLIEIPVHYGGEFGEDLFE
                     VAQFHNTTPEEIIRRHTEPTYTVFMIGFQPGFPYLNGLPQNLHTPRRNVPRTKVPAGS
                     VGIGGRQTGIYPFTSPGGWQLLGRTELPLFDIQHNPPTLLTAGDNVRFVAESITL"
     gene            complement(11691..12515)
                     /locus_tag="PXA33_001299"
     CDS             complement(11691..12515)
                     /locus_tag="PXA33_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HDL5120629.1"
                     /translation="MKKLLLFLLFIGNPIFAKSPELMLVEQYQDQDISGWVMSEKLDG
                     VRGFWDGQQLISRQGYPLNPPDYFIQDFPPFAIDGELFSERGKFEEISSIVRSAQPKG
                     WHKLKLQVFDVPNAQGNLIERLATLENYLKQHPTLHIAIIEQIPIKDKAHLQQFYQSV
                     LEQGGEGVIVRNPNAGYIQGRSAQILKIKPVLDEECTVVAHHKGKGKYTDKLGAITCE
                     NQRGRFRIGSGFKDKERENPPPIGSIITYKYRGVTESGKPRFATFWRIRNDQISEN"
     gene            complement(12649..13473)
                     /locus_tag="PXA33_001300"
     CDS             complement(12649..13473)
                     /locus_tag="PXA33_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5120630.1"
                     /translation="MEHFIGLGVAGNFAGHLEQAGEEADFAKVKTVEAVQPKAIFPFY
                     VPAENLGDYQFLSTYPLSNTAINFPSDADNLQIEPEVALICEIGYQDQQVVSLTPTHF
                     AAYNDCSIRRPNANKICEKKNWGASSKGLSAIHLPLDNFNKGGTLDNYNIACFHKRNG
                     ELNTYGIDSPAVEYSYFHQKLLDWIVERMNNQPDEGPMNNISEMLKQANYPTKAIISI
                     GATRYTPFGESNLLQVGDVSMVVVYNVKKYSHSQIEEMAKLETFSEDISALIQTVR"
BASE COUNT         3698 a         3001 c         3037 g         3789 t
ORIGIN      
        1 tttcgatctc gatcccaaaa atgatttttg ggtattgatt tttcaatcga taaattttct
       61 gtgatcagga tcacaaaaat agcagaaaaa atagaaattg aagttgtatt tcattaaaat
      121 aataaaaaat gaagtgattt attcagaatt gatatttaag gagagactat gcgtttaatt
      181 ccgttagaaa ctgatcacga tgtgagtgtt tgggctgctc gttatattgt tgatcgtatc
      241 aatgcattcc aaccaaccaa agaaaaacct tttgtgcttg gcttaccaac cggcggtacg
      301 cctgaaaaaa cctataaaga attaattaaa ttatatcaag ctggtgaagt gagctttaaa
      361 aatgtggtga ctttcaatat ggacgaatac gtcggcttac ctaaggaaca ccctgaaagc
      421 taccacagtt ttatgttcaa gcactttttt gaccacgttg atattccgct tgagaatatc
      481 aatatcctca acggtatggc ggaagacgtg aatgcagaat gcgaacgtta tgaagaaaaa
      541 atccgttttt atggtaaaat ccatctgttt atgggcgggg tcggtgtgga tgggcatatt
      601 gcgtttaatg agcctgcatc ttcgctttct tcccgcactc gaattaaaac gcttaccgaa
      661 gatactcgca ttgcaaactc acgtttcttt aacaatgacg taagccaagt gccgaaattt
      721 gctttaaccg ttggggttgg tacgttgctt gatgccgaag aagtgatgat cttagtgacc
      781 ggccataaca aagccttagc attacaggcg tgtgtggaag gggcggtaaa tcatctttgg
      841 accatcagtt gcttacaatt acatcgccgt ggtatagtgg tgtgtgatga gccggcaacc
      901 caagagctga aagttaaaac agttaagtat ttcaaagagt tagagaaaaa cgtagctcgc
      961 taattctgat tttacaagcg gttaattttg taatattttt tacaaaaacg accgcctgtt
     1021 agcatttaaa gagaagagag aaaatgaacc attatgcctt aacaaactgt gtgatttaca
     1081 ccggtgaaca agtgctttac caacacgcat tagtggtgaa agaagataaa atcgactcca
     1141 ttgttttgga agaaaattta ccgctggatc tgcaaaaaat cgacctaaaa ggagcgaatc
     1201 tttcagccgg ttttattgac ttgcaactga acggctgcgg tggggtgatg tttaatgaag
     1261 aaatcactac ccgcacttta gaaattatgc aggaaaccaa tctgcgttca ggcacaacca
     1321 gctatttacc gacttttatt acctcgcctg atgagggagt gaaacaagcg gtggcggtaa
     1381 tgcgtgagta tttgtcgaaa cacaaaaacc aagcactcgg cttgcatttg gaaggacctt
     1441 atctaagtgt ggcaaagaaa ggagtacacc gtccggaatt tatccgtgaa atcagccctg
     1501 aaatgttaga tttcttatgc gataacggcg atgtaattac gaaaatcacg attgcagcag
     1561 aaaacccgac cgcagaccat atcaaacgct ttaatgatgc aggaattatt gtatctatcg
     1621 ggcattccaa tgccactttt gctcaaacca aacagcgaat tgccgaaggg gcagcgtttg
     1681 ctacgcattt acacaatgca atgtcgccgg tcagcaacgg gcgtgaaggc ggtgtagtag
     1741 gggcggtgtt ggatagcgat atttacaccg gcattatttg cgatggcttg cacgttgaat
     1801 ggggcaacat tcgcattgcc aaaaaagcca aaggcgataa attagtgatt gtcagcgatg
     1861 ctgttgctgc cgcaggaagt gatattgagt cgtttatttt tgtcggtaaa aaagtcacgg
     1921 tgaaagatgg caaatgctac gatgaattcg gagcattggg cggtgcggca atcacgatga
     1981 tcgaatccat tcgcaacgta gtgcaacatt gcgatattag cctgaatgaa gcaatccgaa
     2041 tgagtaccct ttacccggca aaagcgattg gggtagagga aacactcggc tcacttgaag
     2101 ccggcaaaat tgccaacctt gcgattttcg ataatcaatt tgaagtaaaa gcgactgctg
     2161 taaacgggca attacagtgg cattaatttt gctacaaatt ctaagcataa cagcatataa
     2221 tccaaaggca ggcattgtgt ctgcctttta tttataggga gaataacatg caatttaaag
     2281 attacttagc cacttttcca actattgatc atctagccgg cttaaacgtg ataaatagcg
     2341 gtgaagtgat tcaacatatt cctgcgattg aaggtaaatt aggttcacta aaactttaca
     2401 atgcactgtt tgcgaaattt gatgggaaat taaccgctag tgcagcaaaa caagggttgg
     2461 aatggtttgc cgaacacgtt gaagatgcga aaaataatgt gggtaagcac ccgaatattg
     2521 atctattatt tagggtaatt gagcaagatt tatctttaag tttagagccg attgttaaat
     2581 aagattgcac tagtgaaaaa taacaagcgg tcattatctg caaaattttt gcaaataatg
     2641 actgcttgtg tttaagaggc ttatttcacg gtaatgcgtg caaatttgcg tttgccgact
     2701 tggtaaacaa aagtgccttt ttgagcagtt tgtttcacat cttccacttt ttcaccatca
     2761 attttaaccc cgccttgttg ggctgagcga attgcctctg aagtggacgg tactaagccg
     2821 gcttctttca gtagagccgc taggccgatt tcaccctcaa aggtaaattc aggcacttca
     2881 tcaggaattg cccctttttg gaaacggttg ataaactctt gctctgctgc atctgctgcc
     2941 gcttcatcgt ggaaacgagc gataatttct tttgccagta gaattttcac atcacgcggg
     3001 tttttgccat tttcaacatc cgcttttaat tgtgcaattt cgtctaatgg acggaatgag
     3061 agcaggttgt accaatccca cattaaatcg tctgaaatcg acataatttt gccgaacatt
     3121 tcgcttggag cttcagttac accaatgtag ttgccgagag atttcgacat ttttttctcg
     3181 ccgtctaagc cgacaagtag tgggagtgtc attgccactt gtggtttttg accggctgat
     3241 ttttgtaact cacgtccaac taacaagtta aaggtttggt ctgtaccacc taattccacg
     3301 tcagctttca gttctaccga gtcgtgacct tgtagtagcg gataaataaa ttcgtggatt
     3361 gcgatcggtt gttggttcgt gaaacggttt ttgaaatctt cacgttctaa cattcgagca
     3421 acggtgtagt tggaggctaa gcggatcata ccttccgtac ctaacttgcc taaccattcg
     3481 gagttgaata cgatcttggt tttttgtgga tctaaaattt tgaagatctg ctctttatag
     3541 gtttcggcat tgcgtaacac gtcttcacgg ctaagtggcg gacgggtaga gttttttcct
     3601 gatggatcgc ctaccatacc ggtgaaatca ccgattaaga aatagacttc gtgtccgaat
     3661 tgttggaatt gacgaagttt gttcagcaca acagtatgac ctaagtgaat atccggtgca
     3721 gttgggtctg cccctaattt aatacgcaat gggcggtttt cttttaattt ggtgattaaa
     3781 tcttcttctg aataaatatt ttccacgcca cgtttaagtt cggcaaggac tgattcgata
     3841 gcttgagtca tttatcttcc ttttagtctt gaatataaaa aatggatcta ttatagcggt
     3901 atgaattgaa attgataggc tataggcgaa aaaaaacgcc gtattttgtg ggggaatacg
     3961 gcgtaaagag ttggagtgat tacctattat ttttgaagtg atttaatgat aatgattatc
     4021 atctgttaag tcaatacttt ttttgaaaat tttcaataag aattattctc atcaattaag
     4081 taagatggat actacggcaa ttagtgaaga atgtacagcg gaattttcgt attgtaagag
     4141 tgttggtaag cggtattttt agaaaaaacc tccgagagcg gttatttttc ggaggttttt
     4201 tgcaaatgag gatttacatc gttgaaatgt atttcaacat cacccccgct gcaatcgcag
     4261 agccaatcac gccggcaacg tttgggccca ttgcgtgcat taacaagaag ttttgtttat
     4321 cgtactcttg tccgactttg tttgataccc gtgcagccat cggcacggca gaaacacccg
     4381 ctgagccgat aagtgggttg attttgtgct gactaaagcg gttcattatt ttcgccataa
     4441 tcacaccgct tgcagtgccg atcccaaagg caatcacgcc caatactaaa atgcctagtg
     4501 tttgcggttg taagaatttg tctgcgacca attttgcccc caccgataac cctaagaaaa
     4561 tcgtcacgat attgattaac gcattttggg cggtgtcgtt taagcgttct actacgccac
     4621 tggttcgcag taagttgcca aagcagaaca ttcccaataa cggtgcagca tccggcagta
     4681 agagtgccac taataatagc aacacgatag ggaaaataat tttctcacgg ttgcttacat
     4741 tacggagctg aaccattcgg attttacgtt cttgctcggt ggttaaggct ttcataatcg
     4801 gcggctgaat cagtggcact aacgccatat aagaataagc cgctaccgca atcgcaccta
     4861 ataattcagg ggcaagtttg ctggcaagat aaatcgcggt cgggccatct gccccgccga
     4921 taatcccaat cgcagcggct tgtggcaatg tgaagtcgat aatgcctaac cagtttagcc
     4981 ctaatgcacc cagtacggta gcaaaaatcc caaattgagc cgcagcccca agcattaaca
     5041 ttcgagggtt ggcaagtaaa gggccgaaat cagtcatcgc cccgacaccc ataaagataa
     5101 taagcggagc aacgccgtag ccgatagcaa ctttatagaa taatgccaac acaccggcgg
     5161 tatagcccat atcgccagcc attacttcaa gctcattttg aacagaggga gcagccagtg
     5221 cgatggcatc tttaatcgaa tgcagatcaa ggcttgtact atttacctta gcagcaataa
     5281 ttgccagttg ctcggcagaa ccgctgtgga gtaaattgtc taatgccgtc attgccatac
     5341 ctgcttccgg aatgtttgac atcaaaccac caaagccaat aggcagtagt aaaagcggtt
     5401 cgaattttcg ggcgatggca agccaaagca acactaaact gatgacaatc ataatcgctt
     5461 gccccagttc taaatgcatt agccccatac ctttaaaaag tgcaataata ctttccatac
     5521 cggactccta ggcaatactc attaaagtat catttaccgc cacaacatcg ccttgtttga
     5581 cattaatgcc ttgaatcgtg ccggattttg ccgcacaaat ttgggtttcc attttcattg
     5641 cttcaagaat cagtaacaca tcgccttcag caacttgttg cccctctttc acttccactt
     5701 tcaaaatgtt ccctgccatt ggagcattca ccggttcggc attggctgat agcgtatttg
     5761 cagattttgg tgcagaatcg accgcttgcg gagccggtgt tgctggtgtt ggagcgatat
     5821 tggttatatc gccaccttca ctcaccttca ccacaaaggc tttgccttca agctcaacgg
     5881 tataaatggc tgaaccggat tgtgtttggg cttttggtgc agaagcagcg gaggcttgtg
     5941 ccggttttgc ttcatcagca cttggcactg gctcaaaggc agacggatta ttgcggtttt
     6001 ccaaatattt ccagcccact tgttggaaga gtgccacaat caatacatca tcaatcatgt
     6061 tttcagatag cgtaatgcct ttttcttttg cacttgcagt cacttctgcc actaattttt
     6121 ccagttccgg cggaagatga tcggctgggc ggtcggtaat cggctctgct ccctcaagca
     6181 ctctgtcttg caaggctttg tttaccggtg ccggtgtgcg accatattcg ccttttaaaa
     6241 tacctgcagt ttctttggcg atagttttat aacgttcgcc cattaatacg ttcatcaccg
     6301 cttgtgtgcc gacaatttgt gaggttggtg tcaccaacgg aatattgcct aaatcttcac
     6361 gcacacgagg aatttcttgt aaaaccaagt ctaatttatc cgacgcattt tgttgcttga
     6421 gttggctttc gaggttggtc agcatgccac ccggcacttg ggcaaccaaa atacggctat
     6481 ctataccacg gagctgtcct tcaaattttg catatttttt acgcacgtta cggaagtagg
     6541 cagagatttt ctccagagct ggaatgctca agccggtttc atacggcgtg ccttgaagtg
     6601 tggcgacaat agactcggtt gccgggtgac cgtaagtgcc ggacattgat gaaatggagg
     6661 tatcgacacc atcgacaccg gcttcaatcg ctttgagcaa cgccatttca gccataccgg
     6721 tggttgaatg gcaatggaga tgtagctcaa cgtcgtagcg ttttttaatt tcgctgacta
     6781 attcataggc tgccattggg gttaaaatgc ccgacatatc tttgatcacc aacgaatcca
     6841 cgccaatttc caataattgc tcggttacat ccaaccacgt ttgtagggta tgaacagggc
     6901 tggtggtgta actcaaagta ccttgtgcgt gcccgccgtg ttttttgacc gcttgtaatg
     6961 cggcttgcat attgcgaggg tcgttgaggg catcaaatac acggaacaca ctcataccgt
     7021 ttgccaccga acgctcgaca aatttatcta ccacatcgtc ggcataatga cgataaccca
     7081 ataagttttg cccacgtagc agcatttgca ttggggtttt cggcattgct tttttcagct
     7141 cacgtaaacg cacccacgga tcttcgccta aaaaacgaat acaactgtca aaagttgccc
     7201 cgccccaagt ttctaacgac caatagccga tattatccag ctcggaggca atcggtaaca
     7261 tatcgtctaa gcgtaaacga gtcgcaaaaa gggattggtg tgcatcacgc aaaaccacat
     7321 cggtaatcgc aatttttttc ggttgagttg tcataatcaa attccttcta attattttat
     7381 ttttgaaatc ttgttaatgt tgcccgttaa ttcgacgatg atgagcaatc gcagcggcaa
     7441 tcacagggcg taaacgctct aaatcatctg ggggagattg aggcacagtt ttagaggctg
     7501 gaatagaaac aggttcgggg aaatattgat tgataatctt ggaaataaag ctgatggcat
     7561 aaattaaaac cagcaggaac agcataacaa atcccattcc ggctaccatt aaatttatac
     7621 cttccgcaaa gagttctgca ggtgccataa tttatcctta aaatgtagaa atgatgaaga
     7681 aaatgtagaa aaaattcaga tttatttcaa attatttagc ctcaaaatga gtggtagatc
     7741 acaaacttta aacgcaagcg gtcagatttt acaaattttt tacaagagta gttaaaattt
     7801 gcaaaacctg accttaggtt agcttgaagc taagtcgtta tgccattagc cccgaaatat
     7861 attttgaaag ggtcattgca cttagcaccg ccattagaat caccacaatc gcccctgaaa
     7921 cggtcatcca cacagggtgt ttgtagtcgc ccacaatctt ggttttgtga gctgccagca
     7981 gaattaaccc gagggaaatc ggtagaatta agccgttaag cgtccctaca aacaccaaga
     8041 cttgtgccgg tctgccgatg gttgccagca cggcggttga aaccacaata aaggcgacaa
     8101 tgaaacagtt tttgtgtttt tcgataaatg gcgagaatga agtaatgaag gaaaccgagg
     8161 tataagccgc cccaatcaca gaggtaatgg aggctgccca aatcaccacg ccgaagatca
     8221 tcaaaccgac atcgccggca acgtgggcaa atggggtagc cgcagggttt ttcggatcaa
     8281 gttgcacgcc cattgacacc acgcccagca ccgctaaaaa cagtaccaca cgcataattg
     8341 aggcgattaa aatggctgac accgagcttt tgctcacctc ttttaaattc tctttgcctt
     8401 taatgcctgc atcaagcaaa cggtgcgcac cagcaaaggt gatgtatcca ccaacagttc
     8461 cgcccactag cgtgacaatc gcaatcacgt ctaatttttc cggcacaaag gtatgcacca
     8521 ccgcatcgtc gattggcaga ttggctttta cggcaacgaa gatggttaaa gcaatcatca
     8581 caaagcccat aatttgtgca aatctgtcca tcactttacc agcttcatgg aacaggaaca
     8641 cgcccacggc aatcgcaccg ctgaccaccg cacccatttc aggggaaagt ccggtgagaa
     8701 tgtttagccc taaacctgca ccgcccacgt tgccgatatt aaaggctaaa ccgcccatca
     8761 cgataagcag agccagaaag taacctgccc ccggcaatac ggcattggcg atgtcttgag
     8821 ctttcttttc cgatactgct acaattcgcc aaatattcag ttgagcaccg atatcaagca
     8881 agatcgagag taagatcaca aagccgaagc tggcaagtag ggtttgggtg aaggtggctg
     8941 tttgggttaa gaaccccggc ccgatggcgg aggtcgccat taaaaatgct gccccgagta
     9001 gggcgctacg gttgttgttt gacattgtgt tggttccttt tttgttgtta atgagtcatt
     9061 attcgtggat ttatcatggt aatattcccc tctttagcaa agaggggtta ggggagattt
     9121 ggcagtagaa ctcatcgaag tgagagagtt aataatgctc aatttaacct cttgaaatta
     9181 cttattttct ccgtaaatcg cttctgtcaa atctccccca gcccctcttt tctaaagagg
     9241 ggagttcgat ctccaagagt tttatttctg gttttccgtc cattttgact aaaacaggat
     9301 caggctttca ccaaaatccc ctttgcctgt aactcttttt taattcgctc ggcaaatacc
     9361 acggcgtgtt cgccgtcacc gtgtaagcaa atgctgtcgg ctttaatcgc cacattacca
     9421 ccatcaacgg ttggtacagt gccgtctaac accatttgca agacttggct gatcgcttca
     9481 tcatcgcttt ctaccattgc atcatcacgg gtgcgaggca cgagcgagcc gtcagctaaa
     9541 tagtggcggt cggcaaagac ttcggaaatg gtttccaaac cgtgctgctc ggcaatgctc
     9601 aacatcaggc tgccggataa gcccatcagt ttgaggttgg gatcgaactg tttgaccgta
     9661 ttggcgataa gggtggcaag gtgagggtct ttggcggcca tattgtataa agcgccgtgc
     9721 ggtttaacgt agctcatcgg catattttcc gcatcacata aggctttggc ggctcccagt
     9781 tggtagagca gacaggcttt aagttcatca tcgggcagtt gcatattggt acgcccaaag
     9841 ttttccctat ctggaaaact cgggtgagcg ccaactcgca cgctgttttc cttcgcccag
     9901 ttaatcgctt tacgcatttc attgtaatca ccggcgtgca agccacagca gacattcgcc
     9961 gaagtaacga gctgtaataa tgaatgatcg ttgccacaac cttcggcaag atcgacattt
    10021 aaatcaacgt gattcattcg cttttctccg aattgtttcc aaataatttt gatctttttg
    10081 ataaatttcg accgcttgtt cacggcttac ttgctcaaat tgcaccgttg agccgaacgg
    10141 attttgtgcc aaccgcccga gatcggcctg aataatgcag gcaattttcg gataaccacc
    10201 ggtggtttgg gcatctgcca tcagcacaat cggctggccg tccggcggca cttgcaccgt
    10261 accactcggg gctgcgtagg agagcatttc aagggaagcg gtcaattcca acgctttttc
    10321 tgcaaaacgg tagcccatac ggttgctgtt gttttgcagt tgccaccctt gttgccaaaa
    10381 gcgttcacgg ctatctgcgg taaaggcttc atattcgctc gaagcggttg cccgaatgcg
    10441 tgaggtgaaa tcaatcggct caatcgacat caaagaaagt cggctgtctc tgcggcctgt
    10501 ggcgatgtta tcgccggctt ttaacattct gccttgaaaa ccgccaaatt gagctttgag
    10561 agcggtactt gccgaaccta acacctgcgg caccagaaaa ccgcctgcca cgcataaata
    10621 ggcgtaattg ccgagaacaa tccgttttat cgtcagcgtt tgacgggcgt tggcagtgta
    10681 gcggtaatag gaaaaaaccg gcgtgccgtc cagctccgcc tccaccaatg caccggtgag
    10741 gcaaaatggc gtgtcgcaat caaaggtaat ccgcattccg ccaagggtaa gttcaatcgc
    10801 aggtgcatta tttgcatttt ttaacaataa attacccgct tgtaaggcaa ggctgtccat
    10861 cgcaccgccg tggctcacgc ctaaatggcg ataaccataa cgccctaaat cttgaatgtg
    10921 ggcaaaaccc tgtattattt cgatataaat catagcgtta tactctccgc cacaaatcta
    10981 acattatcgc cggctgtaag tagcgttggc ggattgtgtt gaatatcaaa caacggtaac
    11041 tcggttcgtc ctaacagttg ccagccacct ggtgaggtaa agggataaat gcctgtttgc
    11101 ctgccgccga tgccgaccga accggcaggg actttggtgc gaggcacgtt tcggcgtggg
    11161 gtgtgtaaat tttgcggtaa gccgttgagg taaggaaagc cgggttgaaa gccgatcata
    11221 aacacggtgt aagtcggttc ggtatgacgg cggataatct cttcgggagt ggtattgtgg
    11281 aattgggcga cttcaaacag atcttcgcca aattcaccgc cgtaatggac agggatttca
    11341 atcaggcgtg gggattgtgc ggcttgggct tggtcgtttt cccagagttg ttgaaccgct
    11401 tggataaggc gtgtgctatc ggtgccaatg gcgtgaaata gggtaaaatt gttcattcct
    11461 accaccactt ccaacacgcc atctaaggct cttgcctgtt ctgccacccg ccaaatctgt
    11521 tgctgtttgg caagctctgc aggcggttcg gctaagcaga gcagggaatg ttcgctggta
    11581 tgatgaatcg tcagcataat tgccctcaaa ttgttacaaa aaggtaacaa ctttttacgc
    11641 tgatatatcc agactgtcaa ttttattttg ttgaaaatgt gaaatagctc ttaattttca
    11701 gaaatttgat catttcttat tcgccaaaac gttgcaaatc tgggcttgcc tgattctgtg
    11761 acacctcgat atttataggt aatgattgag ccaatcggtg gcggattttc tcgttcttta
    11821 tctttaaaac cgctgccaat ccgaaaacgt ccacgttgat tttcacaagt gattgccccg
    11881 agtttatcgg tatatttccc tttgccttta tgatgggcaa ccacagtgca ttcctcatct
    11941 aaaaccggtt tgattttcaa aatttgggca gaacgacctt gaatatagcc tgcattcggg
    12001 ttgcgaacga taaccccttc gccaccttgt tccagcacag attggtaaaa ttgttgaagg
    12061 tgggctttat ctttaatcgg aatttgctca ataatagcga tatggagcgt tggatgttgc
    12121 ttcagataat tctctaaggt cgccagccgt tcaatcaaat tcccttgtgc atttggcaca
    12181 tcgaacacct gtaatttcag cttgtgccaa cctttgggct gagcggaacg cacaatgctt
    12241 gaaatttcct caaatttgcc ccgttcgcta aacagttcgc cgtcaatcgc aaaaggaggg
    12301 aaatcttgaa taaaatagtc gggtggattg agcggataac cttggcggct gatgagctgt
    12361 tgaccgtccc aaaagcctcg cacgccatcg agtttttctg acattaccca gccactgatg
    12421 tcttgatctt gatattgctc aacgagcatt aactcgggcg atttggcaaa aatagggtta
    12481 ccaatgaaga gtaggaataa gagcagtttt ttcatagtct ctccgaaata gtgcctccga
    12541 atatgcaagg cactataaag gaaagcgtgc aaacagtcag aaatgaatct caatttttcg
    12601 acgcagatcg caaaattttc cgaaaaactg atcgcttgag tgagaaattt aacgtactgt
    12661 ttgaattaaa gcagaaatat cctcgctaaa ggtttccaac ttcgccattt cttcaatttg
    12721 gctatgagaa tatttcttca cattgtaaac gaccaccata cttacatcac ctacttgcaa
    12781 taaattactt tcgccaaaag gcgtgtagcg tgttgcccca atgctgataa tcgctttagt
    12841 tgggtagttt gcctgtttta acatttcgga aatgttgttc atcggacctt catcgggctg
    12901 attattcatt ctctctacaa tccaatcgag caatttttga tggaaatagc tgtactccac
    12961 cgcagggctg tcgatgccgt aggtattcag ctcgccatta cgtttatgga aacaagcgat
    13021 gttgtaatta tccagcgtgc cgcctttgtt gaagttatct aacggaagat gaattgccga
    13081 aagcccttta gacgaagccc cccagttttt cttttcacag attttattcg cattcggacg
    13141 gcggatagag cagtcattgt aagccgcaaa atgggttggc gtgagcgaaa ccacttgctg
    13201 atcttggtag ccaatttcac aaattaacgc cacttcaggc tcaatttgca gattatcagc
    13261 atcactcggg aaattaatcg cagtatttga aagcggatag gtggataaaa actgataatc
    13321 ccccaaattt tccgccggca cataaaacgg gaaaatcgcc tttggctgca ctgcttccac
    13381 tgttttcact ttcgcaaaat ccgcctcctc tccggcttgt tctaaatgtc cagcaaaatt
    13441 gccggccacg cctaaaccga taaagtgttc cataatctac tccttaattg gtaaaagttg
    13501 gataaaattt taccgcttgt aacca
//
LOCUS       DANLRK010000065        13487 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.065, whole genome shotgun sequence.
ACCESSION   DANLRK010000065 DANLRK010000000
VERSION     DANLRK010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 13487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13487
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.065"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            7..279
                     /locus_tag="PXA33_001301"
     CDS             7..279
                     /locus_tag="PXA33_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005823919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HDL5120631.1"
                     /translation="MPLLFEYDPNKSQINLAKYGIDFEQAKLLWEDERKVVLEACIEP
                     ELRYFLIGKIYDKYWTAFFTPRNDVIRLISVRRSRKKEISYYEKYH"
     gene            263..505
                     /locus_tag="PXA33_001302"
     CDS             263..505
                     /locus_tag="PXA33_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HDL5120632.1"
                     /translation="MRNTISAEELERIFDEGKEDILQYCDLSTAHRPGQMKRVNVDFP
                     NWMVEALDKQARHLGVPRQALIKMWIAEKLPVQSPR"
     gene            517..1185
                     /gene="rluA"
                     /locus_tag="PXA33_001303"
     CDS             517..1185
                     /gene="rluA"
                     /locus_tag="PXA33_001303"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HDL5120633.1"
                     /translation="MALIEYNPPLEPFLDEVYRDNHILVINKPSGLLSVPGNRPEYYD
                     SAMTRVQQKYGFTEPAHRLDMATSGILLFALSKAAEKELKRQFREREPKKHYIALLWG
                     KLGEKVGDKGEMNFPLICDWENRPRQKICYERGKKAITHYEVLAHYPNNIARVKLTPI
                     TGRSHQLRVHSLALGHPIIGDKFYANPLAKSLSPRLCLHAESLTITHPITRELMTFTA
                     EPTF"
     gene            complement(1163..1738)
                     /gene="ampD"
                     /locus_tag="PXA33_001304"
     CDS             complement(1163..1738)
                     /gene="ampD"
                     /locus_tag="PXA33_001304"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HDL5120634.1"
                     /translation="MPNLKIEQGCLTGQKQVPSPHFSQRPDQEDISLLVIHYISLPPE
                     EFGGNFIDLFFQGKLEPTAHPYFEEIKDLRVSAHCLINREGEITQYVNFNDMAWHAGV
                     SCFEGREKCNEYSIGIELEGSNNQPFTQAQYQSLANLTKAIQQSYPQITDERITGHCH
                     IAPERKIDPGQYFDWAYYKHLLNEAKKSAQQ"
     gene            1798..2640
                     /locus_tag="PXA33_001305"
     CDS             1798..2640
                     /locus_tag="PXA33_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HDL5120635.1"
                     /translation="MLSYRHSFHAGNHADVLKHIVQLLILRALKEKEKGFLYLDTHSG
                     VGRYSLLSFESEKTGEYLEGVARLWERDDLPEEVAFYIDELKKLNRGGKLKYYAGSPL
                     LAVQQLREQDRAILTELHPNDFPLLRQEFAKAKNVQTKRENGFQQLKSALPPKERRGF
                     VLIDPPYELKEDYELVVKAIEEGYKRFATGVYAIWYPVVLRQHTKRIVRGLMDTGIRK
                     ILQIELAVRPDSDQRGMTASGMIVINPPWQLETQMKKILPYLTEVLVPEGTGSWKVEW
                     ITPE"
     gene            2763..3404
                     /gene="pyrE"
                     /locus_tag="PXA33_001306"
     CDS             2763..3404
                     /gene="pyrE"
                     /locus_tag="PXA33_001306"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HDL5120636.1"
                     /translation="MQQYKHDFIEFALSRNVLKFGEFTLKSGRISPYFFNAGLFNTGR
                     DLAKLGEYYAKAIQASGIEYDVLFGPAYKGIPIATTVAVALANLFDVDKPCCFNRKEA
                     KDHGEGGNLIGSPLTGKILLVDDVITAGTAIRESMEIINANNAQLAGVLIALNRKEKG
                     KGELSAIQEVERDYGCQVFSIVDFDDLVAFIETSPQYAPYLPKMREYREKYGV"
     gene            3578..4667
                     /locus_tag="PXA33_001307"
                     /pseudo
     CDS             3578..4667
                     /locus_tag="PXA33_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607068.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
     gene            complement(4732..5922)
                     /gene="metC"
                     /locus_tag="PXA33_001308"
     CDS             complement(4732..5922)
                     /gene="metC"
                     /locus_tag="PXA33_001308"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HDL5120637.1"
                     /translation="MSNYKNLSTKLVHTGRKKRNTQGAVNPVIQRASSLVFETLEEKK
                     HATQNRYKGALFYGRRGTLTHFSLQEAMCELEGGAGCYLYPCGAAAVTNAILAFVEQG
                     NHVLMTGAAYEPTQEFCNALLKRMGVSTTYYDPMIGEGLKALVQPNTKVLFLESPSSL
                     TMEVPDIPTLVRVAREINPEIVIMIDNTWSAGVLFPALEYGIDISIQAGTKYLVGHSD
                     VMIGTAVSNARCWDKLREGSYLMGQMVDADSAYITARGLRTLGIRLKQHEQSSIAVAN
                     WLAQRPEVKAVYHPALESCPGHEFFKRDFLGSSGLFSFELCEKLSNDKLANFLEHFEL
                     FSMAYSWGGFESLILANQPEEIAKIRPAIERKLTGTLIRIHVGLEAVEDLIADLEQGF
                     NRIR"
     gene            complement(5981..6520)
                     /gene="dsbB"
                     /locus_tag="PXA33_001309"
     CDS             complement(5981..6520)
                     /gene="dsbB"
                     /locus_tag="PXA33_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HDL5120638.1"
                     /translation="MLYFFKNLSQSRSAWFLLMLSCLILEGTALYFQHGMGLVPCVMC
                     IYERVALFGILFAGILGFLGNRYAIIRYLAILIWLGSAFKGLVLSIKHHDYQVNPSPW
                     NQCEFKVNFPQTLPLDQWLPQVFAPGPVNCSQSQWEMFGFSMAQWLIFTFAVYLIVAV
                     IVLISQFKRSRQRNNFMFR"
     gene            complement(6583..8124)
                     /gene="nhaB"
                     /locus_tag="PXA33_001310"
     CDS             complement(6583..8124)
                     /gene="nhaB"
                     /locus_tag="PXA33_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton antiporter NhaB"
                     /protein_id="HDL5120639.1"
                     /translation="MSSIDALFKSFLGKSPEWYKLCILAFLIINPLIYFFINPFVAGW
                     FLVAEFIFTLSMALKCYPLQPGGLLAIEAVVIGMTNPHHIKEEIMANFDVILLLMFMV
                     AGIYFMKQLLLYIFTKLLVAIHSKKILSLSFCLSAAFLSAFLDALTVIAVIISVGTGF
                     YGVYHKVASGNNFEDSTDITNDEKIISNKEILEQFRGFLRSLLMHAGIGSALGGVMTM
                     VGEPQNLIIASQAEWGFIEFLLRALPVSLPVLICGIATCFLVEKYKLFGYGDRLPSKV
                     WVVLARYSQLKEQKMTKQDRLKMLVQAIAGIWLIIGLALHLADVGIIGLTIIIICTAF
                     CGITDEHTLGKAFQESMPFTALLIVFFSVVAVIIDLKLFEPIIAFVLSAEPHSQLSLF
                     YIFNGLLSMISDNVFVGTVYINEAKTALDAGIIDREQFDLIAVAINTGTNLPSVATPN
                     GQAAFLFLLTSSFAPLIKLSYGRMVYMALPYTIVLSIVGFLGLEFLLPSMTEVMINWG
                     WFITR"
     gene            8281..9006
                     /gene="fadR"
                     /locus_tag="PXA33_001311"
     CDS             8281..9006
                     /gene="fadR"
                     /locus_tag="PXA33_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HDL5120640.1"
                     /translation="MGHSDILKAQSPAALAEEYIVKSIWNNHFPAGSDLPAERELAER
                     IGVTRTTLREVLQRLAREGWLSIQHGKPTRVNDIWETSGPNIIGTIIKLDKSYLPVVI
                     ANVVSLRTRMAEAYIPEAIYLAPEESAKLFDGLDELENTAAAYAEFDYKLFRQFTFLA
                     NKPVYALILNSFKSMYHQVAKIFFADENNRQLTLSFYRSLLCTCLAKDSEKAAACMAK
                     NRQSSGEIWQKLSANIPESFGQL"
     gene            9021..10091
                     /locus_tag="PXA33_001312"
     CDS             9021..10091
                     /locus_tag="PXA33_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HDL5120641.1"
                     /translation="MNEIIFSLEPQDNARLQSLCGAFDEHLALIEKSFNLIIARTGFN
                     FTIQSEDDNHYSATLVQNSVKLIKQLYNETAPIKGKIKELDLEDIHLAIQESRMLLQN
                     QWQAGSQGEIAIKTKRGVIKPRSEHQQAYLRNILSHDISFGIGPAGTGKTFLAVAAAV
                     ESLERQEIRRILLTRPAVEAGEKLGFLPGDLGQKIEPYLRPLYDALFEMLGFEKAQKL
                     MERGVIEIAPLAYMRGRTLNDAFIILDESQNTTTEQMKMFLTRIGFNSKAVITGDITQ
                     VDLPRSQKSGLKHAMEVLKEVPELSFNFFDSKDIVRHPVVAKIVQAYDAWEAVDEERK
                     EQRKLEKLALEQEKILAQAEML"
     gene            complement(10217..12223)
                     /gene="tkt"
                     /locus_tag="PXA33_001313"
     CDS             complement(10217..12223)
                     /gene="tkt"
                     /locus_tag="PXA33_001313"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDL5120642.1"
                     /translation="MTERKVLANAIRFLSMDAVQKANSGHPGAPMGMADIAEVLWRDF
                     LKHNPTNPKWADRDRFVLSNGHGSMLIYSLLHLTGYDLSIEDLKQFRQLHSKTPGHPE
                     YGYAPGVETTTGPLGQGITNAVGIAIAEKTLAAQFNREGHKIVDHYTYAFLGDGCLME
                     GISHEACSLAGTLGLGKLIAFYDDNNISIDGHVDGWFTDDTAQRFESYGWQVIRNVDG
                     HNPEQIKFAIENAQAETERPTLIICKTIIGYGSPNKSNSHDCHGAPLGDAEIQAAREF
                     LGWEHAPFVIPAEIYTEWDAKAKGTLAEKDWNAKFAAYEAAYPELAAEFKRRYAGELP
                     ANWEAESKAFIEKLQANPATIASRKASQNAIEAYAPILPEFLGGSADLASSNLTLWSG
                     SKPIRAEHNVDGNYINYGVREFGMSAIMNGIALHGGFIPYGATFLMFYEYAHNAVRMA
                     ALMKQRALFVYTHDSIGLGEDGPTHQPVEQTASLRQIPNLETWRPADQVESAVAWKAA
                     VERKDGPTALIFTRQNLAQQQRTAEQLANISRGGYILRECCEKGSCPDLILIATGSEV
                     DLAMKAADVLDAEGVKVRVVSMPSTNVFDKQDEAYRESVLPRSVTKRVAIEAQLADFW
                     YKYVGFDGRIVGMNSFGESAPADQLFKLFGFTVENVVAKAKEIL"
     gene            12586..12798
                     /gene="rpmE"
                     /locus_tag="PXA33_001314"
     CDS             12586..12798
                     /gene="rpmE"
                     /locus_tag="PXA33_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005625828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HDL5120643.1"
                     /translation="MKQGIHPEYKEITATCSCGNVVKTRSTVGKDLNLDVCGNCHPFY
                     TGKQRVVDTGGRVERFNKRFSIPSSK"
     gene            complement(12853..>13487)
                     /locus_tag="PXA33_001315"
     CDS             complement(12853..>13487)
                     /locus_tag="PXA33_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase family
                     protein"
                     /protein_id="HDL5120644.1"
                     /translation="TVFPKQVHFADYLNYAIFADFLEKNQVNYNLNIPNELSSFANTL
                     WQEKKIRLLLNPQGSMREISTQELSELLSMIDKRYVQHISCLLTNTTNSAEYLSKIKA
                     PFEISLAPNSDILGYCALVNSADIVIAVDGGGVHISCALGKKLLSFYANHPANIARWY
                     PKPKKDVETFMVIADKWTEDNNDTRGFSLVAPAKWLNHQFAKLIEKYTAC"
BASE COUNT         4140 a         2644 c         2864 g         3839 t
ORIGIN      
        1 tatgctgtgc cgttattgtt tgaatacgac ccgaacaaaa gccaaattaa tttagccaaa
       61 tatggtattg attttgaaca ggcaaagttg ctttgggaag atgaacgaaa agttgtactg
      121 gaggcttgca ttgaacccga attacgctat ttcctgattg gcaagattta tgataaatat
      181 tggacagcgt tttttacacc aagaaatgat gttattcgtt taatttcagt tagacgttcc
      241 cgtaaaaaag agatctcata ctatgagaaa taccattagt gccgaagaat tagaacgtat
      301 cttcgatgaa ggcaaagaag atattttaca atactgcgat ctttctaccg ctcatcgccc
      361 ggggcaaatg aaaagggtga atgttgattt cccaaattgg atggtagaag cgttagataa
      421 gcaagccaga catttaggtg tgcctcgcca agccttgatt aaaatgtgga ttgcggaaaa
      481 attaccggtt caatcaccta ggtaagcgag tggcaaatgg cattaattga atataaccct
      541 ccattagaac catttttaga tgaggtttat cgggataacc atattttagt aattaataag
      601 cctagtggtt tattatcggt gccgggcaat cgtcccgaat attacgacag tgcaatgact
      661 cgagtacagc aaaaatatgg ctttaccgaa ccggctcatc gtttggatat ggcaacaagc
      721 ggtattcttt tgttcgccct aagcaaagcg gcggaaaaag aactaaaacg ccaatttcgg
      781 gaacgtgaac cgaaaaaaca ctatattgcg ttactttggg gcaaattagg tgaaaaagtt
      841 ggggataagg gcgaaatgaa ttttccgctg atttgcgatt gggaaaatcg cccccgccag
      901 aaaatttgct atgaacgagg caaaaaagcc attacccatt atgaagtgtt agctcattat
      961 ccgaataata tagcgagagt aaaactcacg cctatcacgg ggcgttcgca tcagctacga
     1021 gtacatagcc ttgcgttagg tcacccgatt attggcgata agttttatgc taatccgctg
     1081 gcaaaaagtt tatcgccccg tttatgtttg cacgcggagt cactaacaat tactcacccg
     1141 attacaaggg agttgatgac gtttactgct gagccgactt tttagcctcg ttcagtaggt
     1201 gtttgtaata tgcccaatca aaatattgcc ccgggtcgat tttccgttcc ggggcaatat
     1261 ggcaatgccc ggtgatgcgt tcatcggtaa tttgtggata gctttgctga atggctttgg
     1321 tcaaatttgc caaagattgg tattgagctt gagtaaacgg ctgattatta ctgccttcca
     1381 gttcaatgcc aatcgaatat tcattgcatt tctcccgccc ttcaaaacaa gagacgccgg
     1441 cgtgccacgc catatcgtta aaattcacat attgcgtgat ttccccctcc ctattaatca
     1501 aacaatgggc ggaaacccgt agatctttga tttcttcaaa ataagggtga gcggttggtt
     1561 cgagtttacc ttggaaaaac agatcgataa aatttccacc aaattcttcc ggcggtaagc
     1621 tgatgtagtg aataaccaac aaggaaatat cctcttgatc aggacgttgg ctaaaatgcg
     1681 gagacggcac ttgtttttga ccggttaaac agccctgttc gatttttaaa tttggcatag
     1741 tctaaatagg gtgaatacgt taaaatatgg ctcattttac tagaaaaaga gaagaatatg
     1801 ttaagttatc gccacagttt ccacgcaggc aaccacgctg atgtgctaaa acacattgtc
     1861 cagcttttga tcttgcgagc attaaaagaa aaagaaaaag gttttttata tcttgatact
     1921 cactccggtg tggggcgtta tagtctgtta agttttgaat cggaaaaaac aggcgaatat
     1981 ttagagggcg ttgcccgttt atgggagcgt gatgatttac cggaagaagt ggcattctat
     2041 attgatgagc tgaaaaaatt aaaccgtggc ggcaagttaa agtattacgc aggctcccca
     2101 ttattggcgg tacagcaact gcgtgagcaa gaccgagcca ttcttaccga acttcacccc
     2161 aatgattttc cgctgttacg acaagaattt gccaaagcga aaaatgtgca aaccaagcga
     2221 gaaaatggct ttcaacaatt aaaatccgcc ctgccgccta aagagcgtcg aggttttgtg
     2281 ttgattgatc caccttatga attaaaagaa gattatgaat tggtggttaa agcgatagaa
     2341 gagggctaca aacggtttgc cactggtgtg tatgccattt ggtatccggt ggtgcttcgc
     2401 caacatacca aacgtatagt gcgaggttta atggataccg gcattcgcaa aattctacaa
     2461 attgagcttg ccgttcgtcc tgatagcgac caacgtggaa tgacggcaag tggtatgatt
     2521 gtgatcaacc caccgtggca gcttgaaacc cagatgaaaa aaatcctgcc atatttaacc
     2581 gaggtgcttg tgccggaagg aacaggttct tggaaggtgg agtggattac gcctgagtaa
     2641 attaaatacg gatagcacaa gcgtggacgc ttgcgccatc ataaattctg atttgcaata
     2701 ttttttagaa aaataaccgc ttgtcttatc aagaagtggg ggaaatataa aaggaaaaaa
     2761 cgatgcaaca atacaaacac gactttattg aatttgcctt aagccgcaat gtgctgaaat
     2821 ttggcgaatt taccttaaaa tcaggcagaa tcagccccta ttttttcaat gctgggcttt
     2881 ttaacaccgg gcgcgattta gccaaattag gcgaatatta cgccaaagcc attcaagcca
     2941 gcggcattga gtatgatgtg ttattcgggc cggcttataa aggcattccg attgcaacca
     3001 cggttgccgt ggcactagcc aacctgttcg atgtggataa accttgttgc tttaaccgca
     3061 aagaggcaaa agatcacggt gaaggcggta atttaatcgg ttctccctta acgggcaaga
     3121 ttttgctggt ggacgatgtg attaccgcag gcacggcaat tcgtgagtcg atggaaatta
     3181 tcaatgccaa taatgcccag cttgccggtg tgctgattgc gttaaaccgt aaagagaaag
     3241 gcaagggcga gttgtctgcg attcaagaag tcgaacgtga ttacggttgc caagttttct
     3301 cgattgtgga ttttgatgat ttggtagcat ttattgaaac gtcgccacaa tacgctcctt
     3361 atttgccgaa aatgcgtgaa tatcgtgaaa aatacggtgt ttaacttttg caaaaaaaca
     3421 gcaaaaactc accgcttgtc actctctaat ctcaccatta tgaatcactt tcatcttttg
     3481 gtgagatttt ttcaattaaa aagccgataa attgattgtg agctttagct aattggctta
     3541 ggataaactc ctgtaataca ggagttggag aactaaaatg gcacatcaaa aattaccttt
     3601 tcacgcagac accgttggct catatttgcg ttccgatgct tggaaaaaag ctcacgcaga
     3661 ctataaagca ggcaacattt ccctcgaaca acgtgatgaa attgtagaag cggaagtgaa
     3721 aaaattagta caagcatcaa ttagatgcgg gcattcaagt tgttacagac ggcgaatatc
     3781 accgctcttg gtggcatatt gatttcttgg aaaatctgaa cggcattgaa ggctatgtgc
     3841 cggaaaaagc ctatgcgttt aaaggggttg aggttcgccc ttataataca cgttgttgcg
     3901 gtaaagtgtc ttggaatccg aatcaccctt ttattgagca tttcaaaaaa ttcaatgcga
     3961 ttgtaggtgg tcgtgcggtg gcgaaattta ccattccaag tccaaaccaa ttaatgtacc
     4021 cgattcagtg ggatcacggc atttacccaa accgtgagga atttgccaaa gatgtgcaac
     4081 aggcttataa agaggcgatt aaagcgttct atgatgccgg ctgtcgttat ttgcaaattg
     4141 atgatgtcta ttgggggtcg ttgtgtaata acgttggtca accgacattt gaagccgata
     4201 aagctcaagc ggtggcgaat attcaagcca ttcttgccga taagccggaa gatttaacca
     4261 ttaccaccca cgtttgccga ggcaactata aatcttctta tttattgaca ggggcttatg
     4321 atccgattgc cgacggctta tttgctcaaa ccaactatga tgggtatttc ttagaatatg
     4381 atgacgagcg ttcaggcggt tttgagccgt taaaacattt tgccaacaac aaagggcgtg
     4441 tggtattggg cttaattagt tcaaaattcc ctgaattgga agataaggaa gcggttaaag
     4501 cccgtattca agaagcggca aaatatgtgc cgttagagca gctttgccta agcccgcaat
     4561 gtggttttgc ctcaaccgaa gagggcaata ttatgtcgga agcacagcaa tgggcgaaag
     4621 ttcgtcttgt ggaggaaatc gcccaagaag tttggggcga agattaatta gagtgaaaaa
     4681 tgcccgtaac aagcgggcat tttttgtgaa ttttttgcaa attcaagcca tttagcgaat
     4741 gcggttaaaa ccttgttcta aatcggcaat caaatcttcc accgcttcca agccaacgtg
     4801 aatgcgaatt aatgttcccg tcagtttgcg ttcaatcgcc gggcggattt tggcaatttc
     4861 ttccggctga ttggcaagaa tcagcgattc aaacccaccc caagaatacg ccattgaaaa
     4921 cagttcaaaa tgctctagga agttggctaa tttgtcattc gagagctttt cgcaaagttc
     4981 aaatgaaaat agaccgcttg aacctaaaaa atcccgctta aaaaattcgt gccccgggca
     5041 gctttccaac gccgggtgat aaactgcttt cacttcaggg cgttgagcca gccagttagc
     5101 taccgcaatg ctactttgtt cgtgctgttt taagcgaatg cctaaggtgc gtagcccccg
     5161 agcggtgatg taagcggaat cagcatccac catttgcccc attaaataag agccttcacg
     5221 cagtttatcc caacaacggg cgttagaaac cgcagtaccg atcatcacat cagaatgccc
     5281 gactaaatat ttagtgccgg cttgaatgga aatatcaatg ccatattcta aagcagggaa
     5341 cagcacaccg gcagaccaag tgttgtcgat cataatcacg atttctgggt tgatttcacg
     5401 agcaacacgc acgagagtcg gaatatcagg cacttccatt gttagcgagc tcggcgattc
     5461 taaaaaaagc actttggtgt tcggttgcac aagggctttc aagccttcac caatcatcgg
     5521 gtcataataa gtagttgaaa cacccatacg ttttaacagc gcattacaga attcttgtgt
     5581 cggttcgtaa gctgcgcctg tcattaaaac gtggttgcct tgttccacaa aagctaaaat
     5641 cgcattggtg actgccgccg caccgcaagg gtaaaggtag caacctgctc caccttctaa
     5701 ttcacacatc gcttcttgta atgaaaaatg agtgagagtg ccacgtctgc cataaaataa
     5761 tgctcctttg tagcggtttt gtgtagcgtg ttttttctct tctaaggttt caaacactaa
     5821 agatgaagca cgctgaatca ccgggttaac tgctccttgt gtgttacgtt ttttacgccc
     5881 tgtatgaaca agtttggtgg aaagattttt atagttagac ataacttttc ctaaagtaaa
     5941 atgcataggg gcaaatcaac atttgcccct ttattaataa ttaacgaaac ataaagttat
     6001 ttctttgacg gctacgttta aattggctga ttagcacaat gaccgcaaca attaagtaaa
     6061 ccgcaaaagt aaagattagc cattgtgcca ttgaaaagcc gaacatttcc cattggcttt
     6121 ggctacaatt aaccggaccg ggggcaaaca cttgtggtag ccattggtca agcggcaagg
     6181 tttgcgggaa attaacttta aattcgcatt gattccatgg tgatggattg acctgataat
     6241 cgtggtgttt gattgaaagt actagccctt taaatgcgct accaagccaa attagaattg
     6301 ccaagtagcg aataatcgca tatctattac caagaaatcc taaaataccg gcaaataaaa
     6361 taccaaataa agcaactcgc tcataaatac acattacaca aggtactaat cccatcccat
     6421 gttggaaata aagcgctgtg ccttcaagta taagacagct taacattagt aaaaaccatg
     6481 cactgcgaga ttgtgataga tttttaaaaa aatagagcat agttattcct cattttgtga
     6541 acttaaggca ttctacctta tttggtatga gttatctata aactaacgtg taataaacca
     6601 tccccaattt atcattactt cagtcataga aggtagtaag aattctaacc ctaagaaacc
     6661 gacaatagat agaacaatgg tataaggtaa tgccatatag accattctgc cgtaagacag
     6721 tttaattaac ggcgcaaagg aggacgttaa taagaataag aaagcagctt gtccgtttgg
     6781 ggtagcaaca gaaggaagat ttgtgccagt attaattgca actgcaatca ggtcgaactg
     6841 ctcacgatct ataatgccgg catctagtgc ggttttagct tcattaatgt aaacagtacc
     6901 gacgaagacg ttatctgaaa tcatcgagag caaaccattg aaaatataga ataatgaaag
     6961 ctgtgaatgt ggttcggcag ataacacaaa ggcaatgatc ggctcaaata attttaaatc
     7021 aataatcacg gcaacaaccg agaaaaacac aataagcaat gcagtaaaag gcatagattc
     7081 ttggaatgct ttccctaaag tgtgttcatc agtaatgcca caaaatgcag tgcaaataat
     7141 gatgatggtt aagccaataa tccctacatc ggcaagatgt agcgctaagc caataattaa
     7201 ccaaattcca gcaatggctt gcaccagcat ctttaagcga tcttgtttcg tcattttttg
     7261 ttcttttagt tggctatagc gagcgagtac gacccaaact ttgctaggaa gccggtctcc
     7321 ataaccaaat aatttatatt tttctacaag aaagcaggtc gcaatcccac aaattaacac
     7381 gggtaagcta actggtaatg ctctaagtaa gaattcgata aatccccatt ccgcttggga
     7441 ggcaataatt aagttttgtg gttctcccac catggtcatt acgccaccta aagctgagcc
     7501 aataccggcg tgcatcaata aacttcttaa aaaaccacga aattgctcta aaatttcttt
     7561 attagagatg attttttcat cattggtaat atcagtagaa tcttcaaagt tattacctga
     7621 tgcgacttta tgatatacgc cgtagaaacc tgttccaacg ctaatgatta cagcaataac
     7681 cgttaaagca tcaagaaagg cggataaaaa agcagcgctt aagcagaaag aaagggaaag
     7741 aatttttttc gaatgaatgg cgacaagcag tttggtgaaa atataaagca ataattgctt
     7801 cataaagtaa ataccggcaa ccataaacat taaaagaaga ataacatcaa agtttgccat
     7861 aatttcttct ttaatgtggt gaggatttgt cataccgatt acaactgctt caatcgctaa
     7921 taaaccacct ggctgtaatg gataacattt taatgccata gagagagtaa aaataaattc
     7981 agcgactaga aaccagcctg caacaaaagg attgataaag aaataaatga gaggattgat
     8041 aattaaaaat gccaaaatgc acaatttata ccactcgggg ctttttccta agaaactttt
     8101 gaacagagca tcaatgctac tcatataaag gacctcaaaa tttgtaaaca gatattacaa
     8161 ttgtaatata gtttagatta aaggataata gtattactga actttttagt cgcaataatt
     8221 gattagaaat aaagaatagt tagattttag ataagcatat tttttgcttg gggggcatta
     8281 atgggtcaca gtgatatttt aaaagcgcaa agcccggcag cgttagcgga agaatatatt
     8341 gtaaagagta tttggaataa tcatttccct gccggctctg atcttcctgc cgagcgagag
     8401 ttagcagagc gcattggggt aacaagaaca actttacgtg aagtactaca acgtctagcg
     8461 cgagaaggtt ggttaagtat tcagcacggc aagccaacaa gagtgaatga tatttgggaa
     8521 acttcggggc ctaatattat tggcactatt attaagctag ataaaagcta tcttccagtg
     8581 gtgattgcca atgtggtgtc attacgtact cgtatggcgg aggcttatat tccagaagcg
     8641 atttaccttg ctcctgaaga aagtgctaaa ctctttgatg gactagatga attagaaaat
     8701 acagcagctg cttatgctga atttgattat aagttattca gacaatttac ttttttagcc
     8761 aataagcctg tgtatgcgtt gatcctaaat agctttaaat caatgtatca tcaagtcgca
     8821 aaaattttct ttgctgatga aaataatcgt caattaacgt taagctttta ccgttcatta
     8881 ttatgtactt gtcttgcaaa agatagtgaa aaagcagccg cttgtatggc taaaaatcgc
     8941 caatctagtg gtgaaatttg gcaaaaatta tccgctaata ttcctgaaag ctttggtcag
     9001 ctttaacaga aggtagaatt ttgaacgaaa ttatttttag cttagaaccg caagataatg
     9061 ctcgtctgca atcgctttgt ggtgcatttg atgagcattt agccttaatt gaaaaaagtt
     9121 ttaatctcat tattgctcga acaggcttca actttacgat tcaatcggaa gatgacaatc
     9181 actattctgc taccttggtt caaaatagtg tgaagctgat taagcaactg tataatgaaa
     9241 ctgccccgat taaaggcaaa attaaggaat tggacttaga agatatacat ttggcgattc
     9301 aggaaagccg aatgctacta caaaaccaat ggcaagccgg ctcgcaaggc gaaatagcga
     9361 ttaaaaccaa gcgaggggtg attaaacctc gtagcgaaca tcaacaagcc tatttacgaa
     9421 atatattaag ccacgacatc agttttggta tcgggcctgc tggcacaggc aaaacctttt
     9481 tagcggtagc ggcagcggtg gaatcgcttg aacgccaaga aatccgccgt attttactta
     9541 cccgtccggc ggtggaagcc ggtgaaaagc tcggtttttt acccggcgat ttagggcaaa
     9601 aaattgagcc ttacttacgc ccgttatatg atgccctgtt tgaaatgctt ggctttgaaa
     9661 aagctcaaaa gttgatggaa cgtggtgtga ttgaaattgc cccacttgct tatatgcgag
     9721 ggcgtacttt aaacgatgcg ttcatcattt tagatgaaag ccaaaacacg actaccgagc
     9781 aaatgaaaat gttcttaacc cgtattggct ttaattccaa agcggtgatt accggcgata
     9841 ttacccaagt ggatttgccc cgtagccaaa aatcaggctt aaaacacgca atggaagtgc
     9901 taaaagaagt gccggaactc agctttaatt tctttgacag caaagatatt gtccgccacc
     9961 ctgtggttgc caaaattgtg caagcctatg atgcgtggga agcggttgat gaagagcgta
    10021 aagaacagcg aaaactggaa aaattagctt tagagcaaga aaaaattctt gctcaagctg
    10081 aaatgttata gcttacaagc ggtcatattt cgctaatttt ttgcaaaaaa tcgctgaaat
    10141 ttcaccgctt atcataacag ttgttcaaca aaaaagcggg aagttaattc ccgcttttct
    10201 ttatcttatt gtttcattat aagatttctt ttgctttagc aaccacgttt tcaacggtaa
    10261 agccgaagag tttgaataat tgatctgctg gtgcggattc accaaagcta ttcataccta
    10321 caattcgacc atcaaagccg acatatttat accagaagtc agctaattga gcttcaatcg
    10381 ccacacgttt agtgactgaa cgtggtaata ccgattcacg ataggcttca tcttgtttgt
    10441 caaacacgtt ggtgcttggc attgaaacta cacgcacttt cacgccttct gcatctaaca
    10501 catcagcggc tttcattgct aaatctactt cagaaccggt tgcaattaag attaaatccg
    10561 ggcaactgcc tttttcgcaa cattcacgca agatatagcc accacgagag atgttcgcta
    10621 attgctccgc agtacgttgt tgttgagcca gattttggcg ggtaaagatt agtgctgtcg
    10681 ggccgtcttt acgttctacc gccgctttcc aagcaacagc agattctact tggtctgccg
    10741 gacgccaggt ttctaagttt ggaatttggc gtaatgaagc agtttgttct accggttggt
    10801 gggtcgggcc gtcttcacct aaaccgatgg agtcgtgtgt ataaacaaat aaggcacgtt
    10861 gcttcattaa ggctgccata cgcaccgcat tgtgtgcata ttcgtagaac attaagaagg
    10921 ttgcaccgta aggaatgaaa ccaccgtgta gggcaatccc gttcataatc gcagacatac
    10981 cgaattcacg cacgccgtag ttgatgtagt taccatctac attgtgttcc gcacgaatcg
    11041 gttttgagcc agaccataag gttaagttag agcttgctaa gtcagctgag ccgccgagga
    11101 attcaggtaa gattggcgcg taagcttcga ttgcattttg tgatgcttta cggcttgcaa
    11161 tggttgccgg atttgcttgt aatttttcaa taaaagcttt gctttcagct tcccagttag
    11221 ccggtaattc gcctgcgtaa cggcgtttaa attcagcggc tagttcaggg tatgccgctt
    11281 cataagcagc aaatttagcg ttccaatctt tttctgctaa tgtacctttt gcttttgcat
    11341 cccactctgt ataaatttca gctggaatca caaatggggc atgttcccaa cctaaaaatt
    11401 cacgagcggc ttggatttca gcatcaccca atggcgcacc gtggcaatcg tgagagtttg
    11461 atttatttgg tgaaccatag ccgataatgg ttttacaaat aattaaagtt ggacgctcgg
    11521 tttcggcttg tgcattttca atcgcaaatt tgatttgttc tgggttatgg ccatcgacat
    11581 tacggataac ttgccagcca taagactcaa agcgttgtgc ggtatcatca gtgaaccaac
    11641 catcaacgtg gccgtcgata gagatattgt tatcatcgta gaaagcaatc agtttgccta
    11701 agcctaatgt accggcgaga gagcaagctt cgtgtgagat accttccatt aaacagccat
    11761 cgcctaagaa agcgtaagtg tagtgatcga caattttatg accttcacgg ttaaattgtg
    11821 ctgcaagggt tttttccgca atcgctatac cgaccgcatt ggtaatacct tgacctaacg
    11881 ggcctgtggt ggtttcaaca cccggtgcat aaccatattc agggtgacca ggtgttttag
    11941 aatgtaactg acggaactgt tttaaatctt caatagagag atcatagccg gttaaatgaa
    12001 gtaagctata gattaacatt gaaccgtgtc cgttagacag aacaaaacgg tcacgatctg
    12061 cccatttagg attagttgga ttgtgtttta aaaaatcacg ccataaaact tctgcaatat
    12121 cagccatacc cattggtgcg cctgggtggc ctgagttagc tttttgtaca gcatccatac
    12181 ttaaaaaacg aattgcattc gctagcactt tacgttctgt catttgtgtt actcctaatc
    12241 gagaatgtta tataaaatgg aaaggatatc gaattggatt ctacactatt ttgctagaaa
    12301 aactcaaaaa tggaaatgag aaatgtgatc tgtatcacaa aaaatgatta aattgtgatt
    12361 tgttttgatt ggatttgatt ttaatagacg gttttttcct aatacaatat tttcttgata
    12421 aagcgaatgt ttagtagttg atctaggcaa caaatttatc taaaatacgc actctttttg
    12481 gcgttgagca tttgctcaac atttttatta attacatatg gtacctatca gcggtagaga
    12541 ttctaacaag ataggcggcg atatggcttc ttatgaggtt ccccaatgaa acaaggtatt
    12601 cacccagaat ataaagaaat tacggcaact tgctcttgcg gtaatgtagt taaaactcgc
    12661 tcaacagtgg gtaaagattt aaacttagac gtatgtggca actgccaccc gttctatact
    12721 ggtaaacaac gtgttgttga caccggtggt cgtgttgaac gctttaacaa acgtttcagc
    12781 atcccaagct caaaataatt tgattattgc aaaccccgct aatgcggggt tttgtttttc
    12841 ttatatatcg aattaacaag cggtgtattt ttctatcaat tttgcaaatt ggtgatttaa
    12901 ccattttgca ggagctacca gagaaaaacc tcgagtatca ttattatcct cggtccattt
    12961 atctgcgatc accataaaag tttcaacatc tttcttaggt tttggatacc agcgtgcaat
    13021 attagctgga tgattggcat aaaatgagag caatttcttg cctaatgcac aagaaatatg
    13081 gacaccacct ccatcaactg caattacaat atcagcagag tttactaatg cacaataacc
    13141 taaaatatca ctattaggtg ctaggcttat ttcaaatggg gctttaattt tcgacaaata
    13201 ttcggcacta ttggttgtat tggtcagcaa acaactaata tgttgaacat atcttttatc
    13261 aatcatagat aatagctctg ataattcttg agtagaaatt tcacgcatac taccttgagg
    13321 attcaatagt aatcgaattt tcttctcttg ccatagcgta tttgcaaaac tgcttagttc
    13381 attaggaatg tttaaattat aattgacctg atttttttct aaaaaatcag caaaaatagc
    13441 atagttgaga tagtcagcaa aatgaacctg ctttggaaag acagtgg
//
LOCUS       DANLRK010000066        13303 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.066, whole genome shotgun sequence.
ACCESSION   DANLRK010000066 DANLRK010000000
VERSION     DANLRK010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 13303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13303
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.066"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(201..1550)
                     /locus_tag="PXA33_001316"
     CDS             complement(201..1550)
                     /locus_tag="PXA33_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HDL5120645.1"
                     /translation="MLLFIMFAAIILLLVLIMKFKVHAFVALIIVSLLTALAAGIPVD
                     KILPTLLNGFGSTLASVALLVGLGAMIGRLLEITGGAKVLADTLINKFGKERAPFALG
                     VASLLFGFPIFFDAGLVVMLPIIFSVAKQFGGSVLRYAFPSAGAFAVMHAFLPPHPGP
                     VASGDLLGVNMGLLVLVGLVCAIPTWYIGTYLFSQFISKRIHVDLPKAFLNGLSANEM
                     AVQNPPSFGKVLTVLVLPIILILFDTGLNTLSVAKVIDGSEMWVQSLRLIGKTPVALL
                     ITLIVAIMLLRDNRSFDQIEKICNNALGPICSIVLVTGAGGMFGGVLRASGIGDVLSE
                     MMKDTGMPIVVAAFIIATVFRVAQGSATVALTTTAALIAPTVAAATDLSQFDLCFIVI
                     AIASGATVLSHVNDSGFWLMSRFLEMDEKTTLKTWTMLETAIGLTGFACALIGSWLL"
     gene            1737..2252
                     /locus_tag="PXA33_001317"
     CDS             1737..2252
                     /locus_tag="PXA33_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HDL5120646.1"
                     /translation="MANGKAFILMGVSSTGKTSVGTAVAQRLGIKLIDGDDLHPRANI
                     LKMASGTPLNDADRTPWLERINDAVFSFEQKSEKGIIVCSALKKKYRDQIRQGNADVT
                     FLFLHGSFELVLARMQQRKGHFMKPEMLQSQFNTLEIPQADEPDVIFIDIDGSFDEVV
                     ERCVKAVEPLL"
     gene            complement(2306..3304)
                     /gene="gntR"
                     /locus_tag="PXA33_001318"
     CDS             complement(2306..3304)
                     /gene="gntR"
                     /locus_tag="PXA33_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HDL5120647.1"
                     /translation="MRKPKRPTLQDIATHLGITKMTVSRFLRDPNTVAKETGERIAQA
                     IELFGYIPNRAPDILSNAKSRAIGVLVPSLTNQVFADVIKGIEQITDQASYQTMLAHY
                     GYSEQKEEQRIESLLSYNIDGIILSENHHSPRTLKMLEVANIPVIEIMESGECGIQQA
                     VGFDNISAAQAMVETMIHRGCRQVVYFGARMDKRSQLKMQGYEQAMLKHGLSPHSITT
                     AEHSSFTLGASQLKQALQHIPQLDGIFCTNDDLAIGALFECQRLGIHVPQQIKIAGFH
                     GHDVGQSLTPQLASVITPRLEIGRVAAQELLNRIHGVPQQSSIINLGYQIHLGESV"
     gene            complement(3397..4557)
                     /locus_tag="PXA33_001319"
     CDS             complement(3397..4557)
                     /locus_tag="PXA33_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HDL5120648.1"
                     /translation="MIEQSYKIALLFNANKIYDRGVMQGIGQFIQASQCTWDIFVEDD
                     FVYHKNSIESLSIDGIIADFDDLETVELLKDIDVPIVAVGGSYQNPDYYPDLPYVATD
                     NYALVEAAFLHLKQKGINQFAFYGYPAETEKHWAIERKNAFIQLMEKYEHHPNCYIGD
                     QFHSDNWVELQAKLGEWLQTLPAHTGIIAVTDARARHLLQTCEHLKIAVPEQLCVIGI
                     DNEELIQYLSRVSLSSVTQGTSQIGYHAAKLLYRKLNNLPVSTKPILIPPVKVEERRS
                     TDYRSLQDPMVIQAMHFIRHRACQGIKVEQVLDHLKISRSNLEQRFKQEMNKTIHQVI
                     HEEKLARATYMLKFTEISTQEIANVCGYPSLQYFYAVFKKEFGKTPKEFRQE"
     gene            complement(4680..5066)
                     /gene="rplQ"
                     /locus_tag="PXA33_001320"
     CDS             complement(4680..5066)
                     /gene="rplQ"
                     /locus_tag="PXA33_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HDL5120649.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMASALVEHEIIKTTLPKAKEL
                     RRVVEPLITLAKADSVANRRLAFARTRNVDTVAKLFNELGPRFAQRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRPEVATEAAAE"
     gene            complement(5102..6091)
                     /gene="rpoA"
                     /locus_tag="PXA33_001321"
     CDS             complement(5102..6091)
                     /gene="rpoA"
                     /locus_tag="PXA33_001321"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HDL5120650.1"
                     /translation="MQGSVTEFLKPHLVNIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSSKEGVQEDILEVLLNLKGLAVKVQNKDDIILTL
                     NKSGIGPVTAADITHDGDVEIVNPDHVICHLTDKNASINMRIRVQRGRGYVPASARLH
                     TQNDDRPIGRLLVDARFSPVDRIAYNVEAARVEQRTDLDKLVIEMETNGTLDPEEAIR
                     RAATILAEQLAAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAETIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIAED"
     gene            complement(6122..6748)
                     /gene="rpsD"
                     /locus_tag="PXA33_001322"
     CDS             complement(6122..6748)
                     /gene="rpsD"
                     /locus_tag="PXA33_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HDL5120651.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIESKCRNRLDVAPGQHG
                     ARKPRLSDYGSQLREKQKVRRIYGILERQFRNYYTEANRLKGNTGENLLVLLEGRLDN
                     VVYRMGFAATRAEARQLVSHKSIVVNGRVVNIPSFQVSVDDVVAVREKSKKQARIKAS
                     LELATQREKPTWLEVDATKMEGVFKRRPERSDLSADINEHLIVELYSK"
     gene            complement(6777..7166)
                     /gene="rpsK"
                     /locus_tag="PXA33_001323"
     CDS             complement(6777..7166)
                     /gene="rpsK"
                     /locus_tag="PXA33_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HDL5120652.1"
                     /translation="MAKTPVRARKRVKKQIADGVAHIHASFNNTIVTITDRQGNALAW
                     ATAGGSGFRGSRKSTPFAAQVAAERCAEMVKEFGLKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            complement(7183..7539)
                     /gene="rpsM"
                     /locus_tag="PXA33_001324"
     CDS             complement(7183..7539)
                     /gene="rpsM"
                     /locus_tag="PXA33_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HDL5120653.1"
                     /translation="MARIAGINIPDQKHTVIALTAIYGIGKTRAKAICAATGIAEDVK
                     IRELSEEQIEKLREEVGKFTVEGDLRREVTLSIKRLLDLGCYRGLRHRRSLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            complement(7677..7790)
                     /gene="rpmJ"
                     /locus_tag="PXA33_001325"
     CDS             complement(7677..7790)
                     /gene="rpmJ"
                     /locus_tag="PXA33_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HDL5120654.1"
                     /translation="MKVRASVKRMCRNCKVIKREGVVRVICSDPKHKQRQG"
     gene            complement(7815..9134)
                     /gene="secY"
                     /locus_tag="PXA33_001326"
     CDS             complement(7815..9134)
                     /gene="secY"
                     /locus_tag="PXA33_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HDL5120655.1"
                     /translation="MAKQPGYQGSTQKGTGELKSRLLFVLGALIVFRIGSFIPVPGID
                     ASVLSELIRQQQGTIIDMFNMFSGGALSRASIFALGIMPYISASIIIQLLTAIHPPLA
                     ELKKEGEAGRRKISKYTRYGTLLLAFIQSIGISLALPNMVHGLVPNPSILFYVTSVVS
                     LVTGTMFLMWLGEQITERGIGNGISLIIFTGIVADLPATIGQTIEQARQGELSFIVLL
                     LVAIIAFAVTYFVVFVERGQRRIVVNYASRQQGRMMAPARTSHLPLKVNMAGVIPAIF
                     ASSIILFPASITQWFGQSEGFEWLFNLSQLLQPGQPLYVVLFTMAVIFFAFFYTGMQY
                     NPRDTADNLKKSGAFVPGYRPGEQTSRYIDKVMTRLTLIGALYIAFVCLVPYIITSLW
                     KVPFQLGGTSLLIVVVVIMDFIAQVQSHLMSQQYDSVLKKANLKGLN"
     gene            complement(9137..9571)
                     /gene="rplO"
                     /locus_tag="PXA33_001327"
     CDS             complement(9137..9571)
                     /gene="rplO"
                     /locus_tag="PXA33_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HDL5120656.1"
                     /translation="MRLNSLSPAEGAKHSAKRLGRGIGSGLGKTGGRGHKGQKSRTGG
                     GVRRGFEGGQMPLYRRLPKFGFTSLKSFHVAEIRLNDLAKVDGNEVTLETLKAANIIT
                     KDILSAKVILAGKIERAVVVKGLRVTKGAKAAIEAAGGSIEE"
     gene            complement(9576..9752)
                     /gene="rpmD"
                     /locus_tag="PXA33_001328"
     CDS             complement(9576..9752)
                     /gene="rpmD"
                     /locus_tag="PXA33_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HDL5120657.1"
                     /translation="MAKIKVTQTRSSIARLPKHKATLKGLGLRHIRHTVELEDTPAVR
                     GMINQVSYMVKVEE"
     gene            complement(9759..10259)
                     /gene="rpsE"
                     /locus_tag="PXA33_001329"
     CDS             complement(9759..10259)
                     /gene="rpsE"
                     /locus_tag="PXA33_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HDL5120658.1"
                     /translation="MSNIEKQAGELQEKLIAVNRVSKTVKGGRIMSFTALTVVGDGNG
                     RVGFGHGKAREVPAAIQKAMEKARRNMINVALNEGTLQHPVKGSHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVRNVLSKAYGSTNPINVVRATIDALENMKSPEMVAAKRGKT
                     VEEILG"
     gene            complement(10274..10627)
                     /gene="rplR"
                     /locus_tag="PXA33_001330"
     CDS             complement(10274..10627)
                     /gene="rplR"
                     /locus_tag="PXA33_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HDL5120659.1"
                     /translation="MDKKVARIRRASRARHLMREQGATRLVVHRTPRHIYAQVIAPNG
                     SEVLAAASTVEKVIKEQVKYTGNKEAAAVVGKIVAERAIAKGIKAVAFDRSGFKYHGR
                     VQSLADAAREAGLQF"
     gene            complement(10642..11175)
                     /gene="rplF"
                     /locus_tag="PXA33_001331"
     CDS             complement(10642..11175)
                     /gene="rplF"
                     /locus_tag="PXA33_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HDL5120660.1"
                     /translation="MSRVAKAPVNIPAGVEVKLNGQLLTVKGKNGELSREIHNAVEVK
                     QEADALTFAPREGIANADAQAGTARALVNNMVIGVTEGFTKKLQLVGVGYRAQMKGNA
                     VALSLGFSHPVEHTLPAGVTGECPSQTEIILKSADKQLIGQVAADIRAYRKPEPYKGK
                     GVRYSDEVVRTKEAKKK"
     gene            complement(11192..11584)
                     /gene="rpsH"
                     /locus_tag="PXA33_001332"
     CDS             complement(11192..11584)
                     /gene="rpsH"
                     /locus_tag="PXA33_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HDL5120661.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKVAISMPSSKLKVAIANVLAEEG
                     YIESVKVVEGVKPELEITLKYFQNKPVVESIQRVSRPGLRIYKRKDELPKVMGGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            complement(11621..11926)
                     /gene="rpsN"
                     /locus_tag="PXA33_001333"
     CDS             complement(11621..11926)
                     /gene="rpsN"
                     /locus_tag="PXA33_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HDL5120662.1"
                     /translation="MAKQSMIARDVKRAKLADKFYAKREELKKIISDVNASDEDRWAA
                     VLKLQTLPRDSSPIRQRNRCRQTGRPHGVLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            complement(11938..12477)
                     /gene="rplE"
                     /locus_tag="PXA33_001334"
     CDS             complement(11938..12477)
                     /gene="rplE"
                     /locus_tag="PXA33_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HDL5120663.1"
                     /translation="MAKLHDYYRDTVVNELKAKFNYSSVMQVPRIEKITLNMGVGEAL
                     TDKKLLDNAVADLAAISGQKPLVTKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLNAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTSA
                     KTDEEGQALLAAFNFPFRK"
     gene            complement(12495..12806)
                     /gene="rplX"
                     /locus_tag="PXA33_001335"
     CDS             complement(12495..12806)
                     /gene="rplX"
                     /locus_tag="PXA33_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HDL5120664.1"
                     /translation="MAAKIRQNDEVIVLTGKDKGKRGKVTKVLPNGKVFVEGINIITK
                     HEKPVPALGKAGGLVKKEAAIDVSNVAIFNPETNKADRVGFRFEDGKKVRFFKSNEKT
                     I"
     gene            complement(12817..13188)
                     /gene="rplN"
                     /locus_tag="PXA33_001336"
     CDS             complement(12817..13188)
                     /gene="rplN"
                     /locus_tag="PXA33_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012728338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HDL5120665.1"
                     /translation="MIQEQTMLDVADNSGARSVMCIKVLGGSHRRYAAIGDIIKVTVK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGAVIRFDGNACVILNNNTEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
BASE COUNT         3839 a         2952 c         2607 g         3905 t
ORIGIN      
        1 caataaaaag ttatatttac agaatgaata cgagactaaa taatgcagca gaaaggtttt
       61 ggaataattt acaaaatatt cagtttataa atgaagatat attaaataaa ccctaacatt
      121 ctataataag aaaaagcggt cgattctctg ccaaattttg caaattttag aagaaaacag
      181 accgcttgtc agggttggaa ttagagtaac cagctgccaa tcaatgcaca ggcaaaaccg
      241 gttaaaccga tggcagtttc aagcattgtc cacgttttga gggtagtttt ttcgtccatt
      301 tcaaggaaac ggctcatcag ccagaaaccg gagtcattga cgtgggaaag caccgtcgca
      361 ccggaggcga tagcaatcac gataaaacag aggtcgaact ggcttaaatc agtcgctgcc
      421 gccacagttg gcgcaatcaa cgctgctgtg gtggttaaag caacggttgc cgagccttgt
      481 gcgacacgga ataccgtagc aataataaag gcggcaacca caatcggcat tccggtgtct
      541 ttcatcattt ccgataatac atcgccaatc ccgctggcac gcaacacgcc accaaacatt
      601 ccgcctgcac cggttactaa gacaatcgag cagattgggc caagggcatt gttacagatt
      661 ttttcgattt ggtcgaaact gcgattatcc cgcaacagca taatcgccac aattaaggta
      721 atcagtaaag caacaggggt tttgccgatt aagcgaaggc tctgcaccca catttcactg
      781 ccatcaatca ctttagcaac ggagagggta ttcaagccgg tatcaaacaa aatcaggata
      841 atcggcagca ccaacaccgt gagtaccttg ccaaaacttg gcgggttttg tactgccatt
      901 tcgtttgccg ataagccgtt taaaaacgct ttcggcaaat caacgtgaat gcgtttgctg
      961 ataaattggc tgaacaggta agtgccgata taccacgttg ggattgcaca aaccaagccc
     1021 accaagacta acagccccat attcacacca agcagatcgc cggaggcaac cgggccgggg
     1081 tgcggaggta gaaaggcgtg cattaccgca aacgcaccgg cagacggaaa cgcatagcgc
     1141 aacaccgaac cgccgaactg ttttgccacg ctaaaaataa tcggtaacat caccaccaag
     1201 ccggcatcaa agaaaatcgg gaagccgaat aacagcgagg caacgcccaa cgcaaaaggc
     1261 gcacgttctt tgccgaattt attgataagc gtatccgcaa gcactttcgc cccgccggtg
     1321 atttcaagca agcgaccaat catcgcccca agccccacca gtaacgccac tgatgccagc
     1381 gtactaccaa aaccattcaa caatgttggc aggattttat ctaccggaat accggcagct
     1441 aatgcagtga gcaggctcac aataatcaat gccacaaaag cgtggacttt aaatttcata
     1501 atcagcacca gcaataaaat gatggcagca aacataatga acagtaacat aggatctcct
     1561 aaggttgaat tgttatcggt aacataacca aaaagcagtt tagagtaaac ggttgcaggg
     1621 cttttatttt aaaaatgtga tctacttcaa gttttctgga aatagcagat gatttttcag
     1681 cataacctgt tacattaacg gctaagttaa cgttaccagt aacacaagga aattcaatgg
     1741 caaacggcaa agcatttatt ttgatgggcg tgtccagcac cggcaaaacc agcgtgggaa
     1801 cggcagtcgc acagcggttg ggaataaaat tgattgatgg ggacgactta caccctcgtg
     1861 ccaatatact gaaaatggca agtggcacgc cactcaatga tgcagatcgc acaccgtggt
     1921 tggagcggat taacgatgca gtgttcagtt ttgaacaaaa aagcgaaaaa gggattatcg
     1981 tctgctcagc attgaagaaa aagtaccgag accagatccg acaaggcaat gccgatgtga
     2041 cttttctatt cttgcacggc tcgtttgaac tggtgctggc tcgaatgcaa caacgcaaag
     2101 ggcattttat gaaaccggaa atgcttcaaa gccaatttaa tacgctggaa attccgcagg
     2161 cagacgaacc agatgtgata tttattgata ttgacggcag ttttgacgag gtggtggagc
     2221 gttgcgtgaa agcagttgag ccgttgcttt aatcacaagt ggtggatttt gcggattttt
     2281 ttgcaaattt aaccgcttgt ccgggctaca cactttcccc caaatggatc tgatagccca
     2341 agttaattat ggagctttgt tgtggtacgc cgtgaatacg gttgagcagc tcttgggcgg
     2401 caactctgcc tatttcaagg cgaggtgtga tgacgctggc aagctgtggc gtgagggatt
     2461 gcccgacatc gtgtccgtga aagccggcga ttttaatctg ctgcggcaca tgaatgccta
     2521 accgttggca ctcaaataat gccccgattg ccaaatcgtc attggtgcaa aaaatgccgt
     2581 ctagctgcgg aatgtgttgc agggcttgtt ttagctggct tgcgccaagc gtgaaagaag
     2641 aatgctctgc agtagtaatg ctgtgtgggc ttaagccgtg tttgagcatt gcctgttcgt
     2701 agccctgcat tttcagctga gagcgtttat ccattctcgc cccgaaatac accacctgtc
     2761 ggcagcctcg atgaatcatt gtctcgacca ttgcttgagc cgcagaaata ttgtcaaatc
     2821 cgaccgcttg ttgaatgcca cattcgccgc tttccattat ctcaatcacc ggaatattcg
     2881 ccacttccag cattttcaag gtgcggggcg agtggtgatt ttccgataag ataatgccgt
     2941 caatattgta cgaaagcagc gactcaatcc gctgctcttc cttttgttcg ctgtaaccat
     3001 agtgggcgag catggtttga tagctggctt ggtcggtaat ctgctcaatg cctttaatca
     3061 catcggcaaa cacttgattg gtaagcgaag gaaccagcac gccaattgca cggcttttgg
     3121 cattcgagag aatatccggt gcacggtttg ggatatagcc aaacagctca atcgcttgtg
     3181 caatccgctc gccggtctcc ttggcaacgg tgtttggatc ccgcaaaaag cgactgaccg
     3241 tcattttggt aatgccgaga tgggtagcga tgtcttgcaa agttgggcgt ttcggttttc
     3301 tcattggaat agataacgaa aaaatttaat ttcgctgatt ataactgaag ataaggcaat
     3361 aattgcaaaa tattcggtga aattgaccgc ttgttactac tcttgcctga actctttcgg
     3421 ggttttacca aactcttttt taaacacggc atagaaatat tgtaaagagg gataaccgca
     3481 cacattggca atttcttggg tcgaaatctc ggtaaatttg agcatatatg ttgctcttgc
     3541 taatttttcc tcgtgaatca cctggtgaat tgtcttattc atttcctgct taaaacgctg
     3601 ttcaaggtta gagcgggaaa ttttaagatg atccagtact tgctctactt taatgccttg
     3661 gcaagctcgg tgacgaataa agtgcatagc ttggattacc attgggtctt gcaaagaacg
     3721 ataatcggtc gaacggcgtt cttccacttt taccggcggg attaaaatcg gcttggttga
     3781 aaccggtaga ttatttaatt ttcgatataa taacttcgca gcgtgatagc cgatttgtga
     3841 tgttccttgt gtgactgaag aaagagaaac ccgagagagg tattgaatca actcttcgtt
     3901 atcaatgccg atcacgcaaa gttgttcagg tacagcgatt tttaaatgct cacaggtttg
     3961 aagcaggtgt cttgcccttg cgtcagtaac ggcaataatg cccgtgtggg ctggtaaggt
     4021 ttgtaaccac tcgcctaatt tggcttgtaa ttcaacccaa ttatctgaat gaaattgatc
     4081 accaatataa caatttgggt gatgttcata tttttccatc aactgaataa aggcattttt
     4141 tcgctctatc gcccaatgtt tttccgtttc tgcaggatag ccgtaaaacg caaattggtt
     4201 gattcctttt tgttttaggt gtaaaaatgc tgcctcaact aaggcgtaat tgtcggtagc
     4261 aacataagga aggtcagggt aatagtcagg gttttggtaa gaaccgccta ctgctacaat
     4321 ggggacgtca atatctttaa gtagctcaac tgtttccaaa tcgtcaaaat cggcaataat
     4381 gccgtcaatc gacaggcttt cgatactatt tttatgatag acaaaatcat cttccacaaa
     4441 aatatcccaa gtacattgtg atgcttgaat aaactgccca atgccctgca ttactcctct
     4501 gtcataaatt ttgttagcat taaaaagtaa ggcaatttta taagattgtt ctatcattac
     4561 ttcctccagt acccactttc aaaagtaact ataaaataaa attatttttt gatcaaaacg
     4621 aataaaagaa taaaaccccg gtatttctac cagggtttta agctcaattt tgattaagat
     4681 tattccgcag ctgcttcagt tgcaacttct gggcgatcca ctaactcaat gtaagccatt
     4741 ggagcattat cacctgcacg gaaaccacat tttaaaatgc gagtgtaacc acccgcacgt
     4801 tgtgcaaaac gtggacctaa ttcattgaat aatttagcaa ctgtatcaac gttgcgtgta
     4861 cgggcaaaag ctaaacggcg atttgcaacg ctgtctgctt tagctaaagt aattaacggc
     4921 tcaactacac ggcgtaactc tttcgctttt ggtaaagttg tcttgatgat ttcgtgttca
     4981 acaagcgcac ttgccatatt acggaacatc gcttggcgat ggctgctgtt acggtttaat
     5041 tgacgtccac tcttacgatg gcgcataacc tattccttct tagaaaatct ataccgaaaa
     5101 actagtcttc tgcaatactt gcaggtggcc agttttcaag gcgcataccg agtgataaac
     5161 cacgagaagc aagaacatcc ttaatctctg taagagattt tttacccaag ttcggggttt
     5221 taagaagttc aacttctgta cgttgtacta aatcaccgat atagtgaatt gtctctgctt
     5281 tcaaacagtt agcagaacga actgtcagct ctaggtcatc tactggacga agaagaatag
     5341 gatcaaattc cggtttttct tctttcactt caggttgacg aacatcacgc aaatcaacga
     5401 atgcagcaag ttgttctgct aaaatcgttg cagcacgacg gatagcttct tcaggatcta
     5461 acgttccgtt agtttccatc tcaataacaa gtttatctaa gtctgtacgt tgttcaacac
     5521 gagcagcttc gacattgtaa gcaatgcgat ctacaggtga aaaacgtgca tctactaata
     5581 aacgaccgat agggcggtca tcgttttgag tatgaagacg tgctgatgca ggaacataac
     5641 ctcgaccacg ttgaacacga atgcgcatat taattgatgc attcttatct gttaaatgac
     5701 agattacgtg atcaggattc acaatttcta catcgccatc gtgtgtgatg tcggctgcag
     5761 ttacagggcc aattccagat ttatttagtg tcaaaataat atcatcttta ttttgcactt
     5821 ttaccgctag acctttaagg tttaataata cctcaagaat atcttcctga acgccttctt
     5881 tgctgctgta ctcatgcagt acaccatcaa tttcaacttc agtaactgca caacccggca
     5941 tagatgaaag taaaatgcgg cgaagtgcgt tacctaatgt atggccaaaa ccacgctcta
     6001 acggctctaa tgtcacttta gcatgagttg aactaacttg ttcaatattt actaagtgcg
     6061 gctttaaaaa ttctgtcaca gaaccctgca ttttttcctc tctttgcggt taagctaact
     6121 attatttaga gtaaagctca acgatcagat gttcattaat atctgctgat agatcagaac
     6181 gctctggacg acgtttaaat acgccttcca ttttagttgc atcaacttct aaccaagttg
     6241 gtttttcacg ttgagttgct aattctaatg atgctttaat acgtgcttgt tttttcgatt
     6301 tttcacgaac agcaacaaca tcatcaacag aaacttggaa tgaaggaatg tttactacac
     6361 gaccgtttac tacgattgat ttatggctaa caagctgacg tgcttctgca cgtgttgctg
     6421 caaaacccat acgataaact acgttatcta aacgaccttc caataatact agtaagtttt
     6481 caccggtatt accttttaag cggttcgctt ctgtataata attacggaat tgacgctcta
     6541 agataccata aatacggcga actttttgtt tttcacgtaa ttgactacca tagtcagata
     6601 aacgtggctt gcgagcacca tgttgaccag gcgctacatc aagtctattt ctacattttg
     6661 attcaatcgc acgaacgcct gattttaaga ataaatcagt accttcacga cggcttagct
     6721 tgagcttagg acccaaatat cttgccattt tctttctcca attcttttaa cgtcaattaa
     6781 acacgacgtt ttttcggtgg acgacaaccg ttatgtggaa tcggagtcac atcagtaata
     6841 ttcgtgatac ggaaacctgc tgcatttaat gcacggattg ttgactcacg acctggaccc
     6901 ggtcctttaa ccataacttc caaattcttt aaaccaaact ctttaaccat ttctgcacaa
     6961 cgctctgcag caacttgagc agcgaatgga gtcgatttac gagaaccacg gaaacctgaa
     7021 ccacctgcag ttgcccaagc tagagcatta ccttgacggt cagtaatagt aacgattgtg
     7081 ttattgaaag atgcgtggat atgagctacg ccatctgcaa tctgtttttt tacgcgctta
     7141 cgtgcgcgaa ctggtgtttt agccattatt tatctactcc gactattttt tgatcggttt
     7201 gcgtggacct ttacgggtac gcgcattagt cttagtacgt tgaccacgta ccggtaaact
     7261 acgacgatga cgtaaaccac ggtagcaacc taagtctaaa agacgtttga tgcttaaagt
     7321 tacttcacga cgtaaatccc cttcgacagt aaatttacca acttcttcac gcagtttttc
     7381 aatctgctct tcagacaatt ctctgatctt tacatcttca gcaatacccg ttgcagcaca
     7441 gatggctttt gcacgagttt taccgatacc gtaaattgca gttaatgcga ttacagtgtg
     7501 tttttgatca ggaatgttaa tgcctgcaat acgggccact atgcactcct attatttaag
     7561 tttattgata tactgatctt gaaaagcccg tttcaggata ctcaaacagt atatcaggac
     7621 gaatgagctg agcagtataa ctgctcagcg ggttctttgc aagaaatatg cgtaaattaa
     7681 ccttgacgtt gtttgtgttt aggatcgcta caaattacac gaaccacacc ttcacgtttg
     7741 attactttac aattacggca cattcttttt actgaagcac gaactttcat tgcttatcct
     7801 ttattaacgg cctattaatt tagacctttt aagttagctt tcttcaacac agaatcatat
     7861 tgttgagaca ttaaatgact ctgaacctgt gcgatgaaat ccataataac aaccactaca
     7921 atcaataatg atgtaccacc taattggaat ggcaccttcc ataacgacgt aatgatgtaa
     7981 ggaaccaaac aaacaaaagc aatatataac gcaccaatta aggttaaacg tgtcattact
     8041 ttatcgatat aacgagatgt ttgttcgcct ggtcggtaac ctggtacaaa cgcacctgat
     8101 ttcttaagat tatccgctgt atcacgagga ttatactgca ttcctgtata aaagaaagca
     8161 aagaaaatca ctgccattgt gaataataca acatacagtg gttgacctgg ctgtaatagt
     8221 tgtgaaaggt taaataacca ctcaaatcct tcgctttgac caaaccattg agtaatgctt
     8281 gccgggaata aaataatact tgaagcaaaa attgccggta ttacccctgc catattcact
     8341 tttaatggta agtgagaagt tcttgcaggc gccatcattc gaccttgttg gcgacttgca
     8401 tagtttacaa ctattcttct ttgtccacgt tcaacaaaaa caacaaaata agttactgca
     8461 aatgcaatta ttgcaactag tagtaagacg ataaatgata actcgccttg acgggcttgt
     8521 tctattgtct gccctatcgt tgctggaaga tctgcaacaa tacctgtaaa aataatcaat
     8581 gagataccgt ttccgatacc acgctcagtg atttgttcgc ctagccacat taggaacatt
     8641 gttcctgtca ctaagcttac tacagaggtt acatagaata atatacttgg attaggaact
     8701 aatccatgta ccatattcgg tagggctagt gaaataccaa tagattgaat aaatgctaat
     8761 aacagtgtgc catagcgtgt atatttactg attttccgac gacctgcttc accttctttc
     8821 tttaattcag ctagaggtgg gtgaatcgct gtcaataatt ggataataat tgacgctgaa
     8881 atataaggca taatacctag tgcaaatata gatgcacggc ttaaagcacc accagagaac
     8941 atattaaaca tatcaatgat ggtgccttgc tgttgtcgaa ttaattcgga taatacagaa
     9001 gcatcaatgc caggaacagg tataaaagaa ccgatacgga aaacgattaa cgcacctaaa
     9061 acaaacaata gtcttgattt aagctcgcca gtcccttttt gtgtactacc ttggtaccct
     9121 ggttgctttg ccatctttat tcctcgatag aaccgccagc agcttcaata gcagctttag
     9181 cgcctttagt tacacgtaag cctttaacaa ctactgctct ttcaattttg cctgctaaaa
     9241 ttactttagc agataaaata tctttagtaa tgatgtttgc tgcttttaat gtctctaaag
     9301 ttacttcgtt gccatctact ttagctaagt cgtttaaacg aatttcagcc acatggaatg
     9361 attttaatga agtaaaacca aattttggca aacgacgata taaaggcatt tgaccaccct
     9421 cgaaaccacg acgaacaccg ccgcctgtac gagatttttg acctttatga ccgcgcccac
     9481 cagttttacc taaaccagaa ccaataccac gacctagacg ttttgcactg tgcttagcac
     9541 cttcagctgg agaaagagaa tttaaacgca ttctattact cctctacttt aaccatataa
     9601 gacacttgat tgatcatacc acgcactgct ggtgtatctt caagttctac agtatgacga
     9661 atatgacgaa gacctaagcc ttttaacgtt gctttatgtt tcggtaaacg agcgatagag
     9721 ctacgagttt gagttacttt aatttttgcc atatccaatt accccaaaat ttcttcaact
     9781 gttttgccac gttttgcagc aaccatttca ggtgatttca tgttctcaag tgcatcgatt
     9841 gttgcacgaa caacgttaat tgggttagtt gaaccatacg ctttagaaag aacgttacga
     9901 acacccgcaa cttctaatac tgcacgcatt gcaccaccag cgatgatacc tgtaccttca
     9961 cttgctggtt gcataaatac gcgtgaacct gtatgtgaac ctttaactgg atgttgtaat
    10021 gtaccttcat ttaaagctac attaatcata ttgcgacgag ctttttccat cgctttttgg
    10081 attgctgctg gaacttcacg tgctttaccg tgaccaaaac ctacacgacc gttaccatcg
    10141 cccactactg ttaaagcagt gaaactcata atacgaccac cttttacggt ttttgaaaca
    10201 cggttaaccg cgattagctt ttcctgcagt tcaccagctt gtttttcgat gtttgacatc
    10261 tcaaattcct ctattagaac tgtagaccag cttcacgagc agcatctgct aatgattgca
    10321 cacgaccgtg gtatttaaaa ccagaacggt caaaagcaac cgctttaata cctttggcaa
    10381 ttgcacgctc agcaacgatt ttacccacta ctgctgcggc ttctttattg ccggtatatt
    10441 taacttgctc tttaattact ttttcaacag ttgaagctgc tgctagtact tctgagccat
    10501 taggtgcaat cacctgcgca tagatatggc gaggtgtgcg atgcacaact aaacgtgttg
    10561 caccttgctc tctcattaaa tgacgtgcac ggcttgcacg acggatacga gctactttct
    10621 tatccatagt gttaccttac tttacttttt cttcgcttct tttgtacgca ctacttcatc
    10681 agagtaacgt acacctttac ctttataagg ttcaggtttg cgatatgcac gaatatctgc
    10741 tgccacttga ccaattaact gtttgtcagc acttttcaga ataatttctg tttgtgatgg
    10801 acattcacca gttacgcctg ctggcaacgt gtgttctact gggtgagaga aacctaaact
    10861 taaagcaaca gcattgcctt tcatttgtgc tctataacca acacccacta attgcaattt
    10921 cttagtaaag ccttcagtaa ccccgattac catattattt actagtgcac gagcagtacc
    10981 tgcttgagca tctgcattag cgataccttc acgtggagcg aaagttaatg catctgcttc
    11041 ttgttttact tcaactgcat tgtgaatttc acgagataac tcgccgtttt tacctttaac
    11101 tgttaatagc tgaccgttaa gttttacctc aacaccggca ggaatattaa caggtgcttt
    11161 tgcaacacga gacatttttc ctacctctct attatgctac gtaacagata atttcaccgc
    11221 cgaggcccgc ttgacgcgca gcacggtcgg tcattacacc tttagatgta gaaacaactg
    11281 cgatacctaa accacccatt acttttggta attcgtcttt gcgtttataa atacgcagac
    11341 ccggacggct cacacgttgg atactttcta caaccggttt gttttggaaa tattttaaag
    11401 taatttccag ttcaggttta acaccttcaa caactttaac gctttcgata taaccttctt
    11461 cagctaaaac atttgcaata gcaactttta acttagatga aggcatactg attgcaactt
    11521 tgttcgcagc ttgaccgtta cgaatacggg tcagcatatc tgcgattgga tcttgcatgc
    11581 tcatgtattt actcccatga ttccaaatta aaagaggtta ttaccagctt gccttcttaa
    11641 gacccggaat ttcaccgcgc atagcagctt cacgaacctt aatacggctt aaaccaaact
    11701 tacgaagtac gccatgtgga cgaccagttt ggcggcaacg attacgctga cggattgggc
    11761 tagaatcacg aggaagtgtt tgtaatttta acactgctgc ccaacggtct tcatcagatg
    11821 cattcacatc agagatgatt ttctttaatt cttcacgttt tgcgtagaat ttatcagcta
    11881 atttagcacg ttttacatcg cgtgcgatca ttgattgttt tgccacgata acctacctta
    11941 tttacggaat gggaaattga aagcagctaa taaagcttga ccttcttcat cagttttcgc
    12001 tgaggtggtg atagtaatat ctaaaccacg aacacgatct actttatcgt agtcgatttc
    12061 agggaaaatg atttgttcac gaacacccat actgtaatta ccacgaccat cgaatgactt
    12121 agcgtttaaa ccgcggaagt cacgaatacg tggaacagcg atagtaatca atctttcaaa
    12181 gaattcccac atacgttcac cacgtagggt tactttgcaa ccgattggat acccttgacg
    12241 gattttaaag cctgcaacag atttgcgagc tttagttact aaaggttttt gaccgctgat
    12301 tgctgctaaa tccgctactg cgttatctaa cagttttttg tcggtcaatg cttcacccac
    12361 acccatgtta agggtaatct tttcgattcg tgggacttgc atgacagatg agtagttgaa
    12421 ttttgctttt aattcattaa ctactgtatc tctgtagtaa tcatgcagtt tcgccatcac
    12481 attactccag ttaattaaat tgttttctcg ttagatttga agaaacgcac ttttttgcca
    12541 tcttcgaatc taaatcctac acggtcagct ttgttggttt cagggttgaa gatcgcaacg
    12601 tttgaaacat cgattgcagc ttctttcttc actaaaccac cagctttgcc taatgcagga
    12661 actggttttt cgtgtttagt gatgatgttg ataccctcaa caaaaacttt accatttggt
    12721 aacactttag ttaccttacc acgtttgccc ttatctttac cggtaagaac aattacttca
    12781 tcgttttgac ggatttttgc agccattaca ttctccttat agtacttctg gagctaaaga
    12841 gatgattttc ataaacttct cagaacgaag ttcacgagtc accggtccaa aaatacgagt
    12901 accgattggt tgctcagtgt tattgtttaa aattacacaa gcattgccat cgaaacgaat
    12961 aactgcgcca tctgggcgac gaacaccctt cttggtgcgc acaacaactg cttttaacac
    13021 atcacctttt ttcactttac cgcgtggaat tgcttctttt acagtaactt taatgatatc
    13081 gccaatagca gcgtaacgac ggtgcgatcc acctagaacc ttgatacaca ttacgctacg
    13141 agcccctgag ttatcagcaa catccagcat agtctgttct tggatcatat tagtgctccg
    13201 ttaaaataaa aaaacaccct ttcgggacga acctatttat acaaatataa atagggtgca
    13261 aaatatacta caaaaaccac tagttaatca agtgaatttt taa
//
LOCUS       DANLRK010000067        13116 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.067, whole genome shotgun sequence.
ACCESSION   DANLRK010000067 DANLRK010000000
VERSION     DANLRK010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 13116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13116
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.067"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(427..1467)
                     /locus_tag="PXA33_001337"
     CDS             complement(427..1467)
                     /locus_tag="PXA33_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDL5120666.1"
                     /translation="MKKQLRKNLTQNDRLQPQAQTEIFSFGDPIPVLDRADILNYLEC
                     SAMYEKWYNPPMSFDGLAKSLRSSTHHESAIITKANILLSTCEVDSRYLSRRDLSSFV
                     KDYLVFGNAYFEVVRNRLGQVQRIESPLAKYVRKGLEAGQFYYVPQRFDHQEHEFAKG
                     SIYHLLEPDINQDIYGLPQYLSALQSAWLNESATLFRRKYFLNGAHAGFVFYMSDASQ
                     KQEDVENIRQQLKQSKGVGNFKNLFVHAPNGKKDGIQIIPIADVSAKDEFFNIKNVSR
                     DDVLAAHRVPPQLMGIIPNNTGGFGNVADAAEVFFITEIEPLQERLKEFNQWLGQEVI
                     KFKPSKLLQRTQ"
     gene            complement(1476..3278)
                     /locus_tag="PXA33_001338"
     CDS             complement(1476..3278)
                     /locus_tag="PXA33_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase ATPase subunit family protein"
                     /protein_id="HDL5120667.1"
                     /translation="MENENTITEYEVLAANPLHDKREAQSKYWAGYTVTEISRQLNIP
                     VSTIASWKKREKWDEISPVGRVEATLESRLNLLIMKESKNNNDYKEMDALRRLLESTA
                     RIKKYSNGGGNEADLNPNIKNRNKGDRKKPEQNAISEEQAELLINGFLDGMFHYQKKW
                     HEAGLTHRIRNILKSRQIGATYYFAHEALVDALVTGRNQIFISASKKQALQFRAYIVA
                     YAKRVADVELKGETITLPNESQLIFLGTNSKTAQSYHGNLYFDEIFWVNRFEEIRKVA
                     AGMASQKQYRITYFSTPSSITHSAYLLWSGKLFNRKRPKAEQVEIDISHANLKNGKKC
                     GDGQWRQIVNIYDAEAGGCNLFDIEQLKLENSPDEFEQLFMCEFIDDNQSVFKFTMMQ
                     RCLVDSMEVWRDYVFTDGYQRPFGNKEVWVGYDPSYTGDRSALVVIAPPKVDGGKFRL
                     LEYRTFKGADFAEQAAEIVAICAKYNVTRLAIDTTGLGVGVYEIVKKERPDAVALTYN
                     VELKSKMVLKGLDIISKGRFEFDSMHAVEVGASFMAIKKQITNSGRQVTYVADRSEEA
                     SHADLAWACLQVFINEPFDGKLEETTGTVDFLDD"
     gene            3428..4255
                     /locus_tag="PXA33_001339"
     CDS             3428..4255
                     /locus_tag="PXA33_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HDL5120668.1"
                     /translation="MTTKSKWFVVATEGATTDGREISREWLTQIEENYDPKNNYGARI
                     NLEHFRWRWFEKDDPHSYSYGDVLAVKTEEREDGKLQLLAEIAPTEALVKLVKDKQKV
                     YTSVEIDLNFADTGEAYLVGLAVTDTPASLGTEYLTFCAGATHNPLADRKQKPENLVS
                     EAVEANLEFTAEPEKGAWLEKFKAMFTKAKTDNDGKFAEHEKAMELLAEQFSKVSTEN
                     DRLQTELSTLQIEFTELQKQAGEFAEKFAKLEQEPSANYTPRPKATGAKAGDVQTDF"
     gene            4269..5297
                     /locus_tag="PXA33_001340"
     CDS             4269..5297
                     /locus_tag="PXA33_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HDL5120669.1"
                     /translation="MKDLTLEKYNAYLARQAELNNLPFNALATGIKFTVQPSVQQKLY
                     EKVRESSDFLKSISFVFVDEQTGETLGLDSAHTVASTTDTSGDGERKTTSIAKLVKQT
                     YHCQQINFDTHINYKQLDMWAKFPDFQQKVANVAAKQRKRDLIMIGFNGTSRAATSDR
                     NSNPLLQDVAKGWLQKMREDAKERVMNGESTDNQVLVGKGQEYANLDALVMDATEELI
                     DEWHRDDTDLVVITGRKLLADKYFPIVNQQNAPTEELAADIVISQKRIGGLKAVRVPF
                     FPANAILITKLENLAIYVQEGTTRKHIENVPKKDRIETYESENIDYVVEDYGCAALIE
                     NITLKDKE"
     gene            5307..5996
                     /locus_tag="PXA33_001341"
     CDS             5307..5996
                     /locus_tag="PXA33_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase endonuclease subunit"
                     /protein_id="HDL5120670.1"
                     /translation="MRMSPARAHFLRKTAESESATPNRENLEGLQGYDLVLAQLKAHQ
                     RQLKKIQSIERKIEFKRNNFEQYRPWVEGSLAKGSGVQDTVITTMLIWSIDIGDYPTA
                     LAIAQYVLMHDLAMPEQFERTPATALVEELAEAAKKARDQKQPFEAAVLKQANDLTAE
                     FDMPDPVRAKLLRELGELIQEAEPQTALEHYQRAIALDPQCGAKGLRDKLEKQLAKQA
                     EEPKPAEEQNE"
     gene            6108..6623
                     /locus_tag="PXA33_001342"
     CDS             6108..6623
                     /locus_tag="PXA33_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HDL5120671.1"
                     /translation="MEQRNHVISIPKVNGYESSKNAIKPTAESDETITNNGFFPNIEL
                     LEVRNAMRIDGTVINERLKQAVIEAMATVNADLKAYRLNAEQAQKANLQACDDEQING
                     ESVLVYKYKRAVYCLAVANLYERYRSYDSTKDGHDKAEELESTAGDLKRDYHFAVRDI
                     LGENRMISELI"
     gene            6620..6832
                     /locus_tag="PXA33_001343"
     CDS             6620..6832
                     /locus_tag="PXA33_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HDL5120672.1"
                     /translation="MNTLIARQDDTLDELIFRHYGQTAGLVEQALEYNPELAHLPRLP
                     IGTVVTMPDIESRFATVAKSSVQLWD"
     gene            6838..7044
                     /locus_tag="PXA33_001344"
     CDS             6838..7044
                     /locus_tag="PXA33_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HDL5120673.1"
                     /translation="MHKTEQASYFGAIFGFFGSLTLSDWGVVVSILVAILTLLLNWAY
                     RAKEYKLKEREFELKLQNQGKKNE"
     gene            7037..7603
                     /locus_tag="PXA33_001345"
     CDS             7037..7603
                     /locus_tag="PXA33_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HDL5120674.1"
                     /translation="MSKSVKVGVFVCSVAAIIGTVKQYYSSEIRTSETGLAIIGNAEG
                     CRRDPYKCPADVITVGIGSTEASGEKINVNHKYTDKEIAERWIKDLKIAERCINRYFN
                     GDKMNENQFSAMVSAAFNMGCYNLRFYPNENGKYIQTTIHKYAMAKDFKAMCNRIPDF
                     NRSGGKVLRGLKIRREKEKALCLGQEVK"
     gene            7600..8055
                     /locus_tag="PXA33_001346"
     CDS             7600..8055
                     /locus_tag="PXA33_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5120675.1"
                     /translation="MIVKLCQMTNKHFSPLMIVLVLVGVLANFVQFRQNESLKADLQA
                     RTTQNQLIQADNRNLANQLENSQIQLEAYQKQVDDLNQKVLAKMKQAEQRSNEILNEL
                     EKHKKWADSAVPPSVGKLLNQRKRAVSHPQIEPDNLSEKAGVPNTGNRD"
     gene            8204..8425
                     /locus_tag="PXA33_001347"
     CDS             8204..8425
                     /locus_tag="PXA33_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HDL5120676.1"
                     /translation="MSDQIDRANELAEKAREAALAKILQNQTACTSLFECEDCGEPIP
                     EKRREMVIGCTRCIECQTIYEHKQKGYRR"
     gene            8422..8907
                     /locus_tag="PXA33_001348"
     CDS             8422..8907
                     /locus_tag="PXA33_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL5120677.1"
                     /translation="MIKPDRLRAVLTQTIDIFQANPEKLILQYDKGKIKSKGSQSHSF
                     EYHYDLELIVVDFPYHPDVLFVPVLNFVRNEQWELLQNPELQDKIEFEIDHNNHESYD
                     IYIRLPLTERVIVKEQDGHLTATHADEPNLADVSPFTRLTEYEVYLKDELIYQWAEPN
                     E"
     gene            8900..9358
                     /locus_tag="PXA33_001349"
     CDS             8900..9358
                     /locus_tag="PXA33_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HDL5120678.1"
                     /translation="MSEPIEQVKTAFDSLLNNISKPRRRLMYQQIGRELARSQRRRIK
                     AQQNPDGSAYEPRKKPKKGVKSKIKSGKMFDKITQPRFMRLRLESEGVSLGYEGGDAV
                     IARIHQQGLIGRVRKDWDLKVKYASRELLGFTDDDLQMIEDYMINILAGS"
     gene            complement(9409..9687)
                     /locus_tag="PXA33_001350"
     CDS             complement(9409..9687)
                     /locus_tag="PXA33_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5120679.1"
                     /translation="MEILFILLGLIGFGALLAIGFTTFAAIFGVVTTVFLSLLSLLAN
                     MVGLSIIVILISLYVLLPLNTFILIALIFSAILGFLCWYHSDRQSVNQ"
     gene            9726..12638
                     /locus_tag="PXA33_001351"
     CDS             9726..12638
                     /locus_tag="PXA33_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL5120680.1"
                     /translation="MSNNLKIQVVLSALDKLTAPFKSVVKQTEKLSQSLKVQRDELKA
                     LEKTQSKMQSFKKMDDSIKKSNETILKQTKHLDNLKNKISQMKNERIELKVKMDAKKR
                     EFQQITKGGSAWSARAAFLDKDFEKMQREYEKLGASISSVSRKRTEENRALKDSRHQQ
                     AKQLLYFRSLRRELRASGINIKAFAQSETNLTDKIAKANQLIDQQKAKLEKLNAVKAR
                     NEKYWATVEKVKNTSERLHNLGQRSMISGAAISAPVVGLGKGVVGMAQTAGKFEQFNA
                     ILEVTEGSAEKAKQSFDWVKQFAVDTPSNLDEAMEAFVKLRAYGLDPTNGLLQTLGDT
                     GAAMGKPVMQAVEAIADALTGENERLKEFGIKGSVVKGTNIIEYAYTDKLGKQQVAKV
                     NKNNRKEIEQTLTRIFNEKYAGAMEKQAKTLVGIWAKIEDYWTNFQMQVMESGAFDWI
                     KNKLQWVLDSLDKMAENGELQKWAEDVGAVIQETVQGLWAFGEKIIEVVKWLASLARE
                     NKGLIATFVKWSAILGSSLTVLGAFAVVASFALYPVARLALGIGKFTGASTLLNKALF
                     DSNDKFRLFNKSLYSSGTTAAFVKSKFNQLATLPNVLGSSFNKLGGGIKMLIPQLKTK
                     VFWLNMLKGILGKLWSAIRFIFSPLKLLLGIFSPIGLAITAISVAGVALYRNWEKVRA
                     FFGGFLQGLSKGLAPVLERFKPLGKLFETISNWVKSTFKWLSDLLTPADKTSESLDKA
                     SSAGEKFGLALAGAIDLVTSPVQWLVDQFKWISENMPSWDTIGEGVKKAATAIVPTEH
                     AKQITKTAEMSNNMYDPNYTPPQLEQKWIGGLVGNGKGRGFATGGYTGNGGKYEPAGI
                     VHKGEYVMTKEATARLGVANLNRLNYGKVAGLTALASSVAFAQPMPAVKIDSRPPLTA
                     SQPSPTVAPVSQNIHITINATGGQDPQAIARLVAMELEKQQRQAQARARSSLRDRG"
     gene            12700..12879
                     /locus_tag="PXA33_001352"
     CDS             12700..12879
                     /locus_tag="PXA33_001352"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5120681.1"
                     /translation="MMFKEKGYDEFVNECVRKGREEHKAGLGFTLEQSRLHIKKTIEQ
                     KSQELNEFQREVVYG"
     gene            12872..>13116
                     /locus_tag="PXA33_001353"
     CDS             12872..>13116
                     /locus_tag="PXA33_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL5120682.1"
                     /translation="MAKITIKPQALSQFDEIVKSVIEFTGFETSGIRLQSDIFDKIEQ
                     IAFMPLSGRAREDGTRETFVRRYRIVYDYDRQADEVI"
BASE COUNT         4205 a         2694 c         2901 g         3316 t
ORIGIN      
        1 gcaccaataa tcattattta tcaataagtt aaaatctata atgtgcagta tatgtatatg
       61 caattaaaga ataacgattt ttcctgacaa gcaatatttt tgcgttgagg tgcgtaaaat
      121 tgcgtaaaaa gatcgagttt ttaagatcgc tttgcgatta tatggggctt gtagggatcg
      181 cctttttgca tttgcgtata aatcgacaaa ttaagtgtgc gggcgaggtg agggatagac
      241 cgcgcggggt tggaggtggt gaagtcgttg aaaaataaag gaaaaattat tttttaagtg
      301 tattttattt gttgttttga tataataaat tgccgaaata tataatctaa tcaggcaaaa
      361 cttttgcttt atatattaaa aaagccccaa tctaggagct gtattgtcag tttgtaaaga
      421 tctgttttat tgtgtccgct gtaacaattt agacggttta aatttgataa cctcttgacc
      481 taaccactga ttaaactctt tcaatcgctc ttgcaatggc tcaatctcgg tgataaaaaa
      541 cacctcggca gcatcagcca cgttgccaaa accgccggtg ttatttggga taatccccat
      601 cagttgtggt ggcacacggt gagcagctaa cacatcatca cggctcacgt tcttgatatt
      661 aaaaaactca tcttttgccg acacatcagc aatcggaata atctgtattc catctttctt
      721 gccgtttggt gcgtggacga acaagttttt aaaattcccc accccttttg actgcttgag
      781 ctgctggcga atattttcaa catcttcttg tttttgtgac gcatcgctca tataaaacac
      841 aaagccggcg tgagctccat ttaaaaaata cttacggcga aacagagtag cactctcatt
      901 taaccacgca gattgcaggg ccgaaagata ttgtggcaag ccgtaaatat cttgattaat
      961 atcaggctca agcaagtgat agattgagcc tttggcaaat tcgtgttctt ggtgatcgaa
     1021 tcgttgtggc acatagtaaa attgaccagc ctctaaaccc tttcgcacat attttgccaa
     1081 aggtgattcg attcgctgta cctgcccaag gcgatttcgc accacctcaa aataggcgtt
     1141 gccaaatact aaataatctt taacaaacga tgacaaatca cggcgtgata aataacggct
     1201 atcaacctcg caagtggaaa gcaaaatatt tgctttggtg atgatcgcac tttcgtggtg
     1261 tgttgatgac cttaacgatt tcgccaagcc atcaaagctc atcggtgggt tataccattt
     1321 ctcatacatc gccgaacact ccaaataatt gagaatatcg gcacggtcta acaccggaat
     1381 cgggtcacca aaactgaaaa tttcggtttg tgcttgcggt tgtaagcggt cattttgggt
     1441 taaatttttg cgcaattgtt ttttcatttt ttgtcctaat catctaaaaa atctactgtg
     1501 ccggtcgttt cttccagttt cccatcaaac ggctcattaa taaatacctg caagcaagcc
     1561 cacgcaaggt cggcgtggct tgcctcttcc gaacggtctg ccacataagt cacttgccgt
     1621 ccgctgttgg tgatctgttt tttaattgcc ataaaactcg caccaacctc aacagcgtgc
     1681 attgagtcaa attcaaatcg tcctttgctg ataatatcca agccttttag taccatcttg
     1741 gatttcagct ctacgttgta agtaagagcc accgcatctg gtcgttcttt tttgacgatt
     1801 tcataaacac cgacaccaag ccctgtggta tcaatcgcca gtctggtcac gttatatttt
     1861 gcacaaatag cgacaatttc tgccgcttgt tcggcaaaat cagccccttt aaaagtgcga
     1921 tactctaata gtcggaattt cccgccgtcc actttcggcg gagcgattac caccaacgcc
     1981 gagcggtcgc cggtgtaaga cgggtcatac ccaacccata cctctttatt gccgaatggg
     2041 cgttggtagc cgtccgtgaa tacataatct cgccacacct ccatcgagtc cacaaggcaa
     2101 cgttgcatca tcgtgaactt gaacacagac tggttatcat caataaactc acacataaag
     2161 agctgctcga actcatcggg cgagttttcc agtttgagtt gctcaatatc aaacaggtta
     2221 cagccacctg cctcggcatc gtagatattc acgatttgtc gccattggcc gtctccgcat
     2281 tttttgccgt ttttcaagtt ggcgtgagaa atatcaattt ccacctgttc cgctttcgga
     2341 cgtttgcggt taaacagctt gcccgaccaa agcagatagg cggaatgggt aatgctcgaa
     2401 ggggtagaaa aataagtaat ccgatattgt ttttggctcg ccatacccgc tgccactttg
     2461 cggatttcct caaagcggtt tacccaaaag atttcatcaa aatagagatt gccgtggtaa
     2521 gactgtgccg ttttcgagtt tgtacccaag aaaattagtt gagactcgtt cggcaaggta
     2581 atggtttcgc cttttagctc cacatcggca acccgtttgg cgtatgccac aatataggct
     2641 ctaaattgca atgcctgttt tttcgaggcg gaaataaaaa tctgatttct accggttact
     2701 aacgcatcaa ctaacgcctc gtgagcaaaa taataagtcg ctccaatttg acgactttta
     2761 agaatattgc ggattcgatg agtcagccca gcttcgtgcc attttttctg ataatggaac
     2821 attccgtcta aaaagccgtt tatcaacagt tcggcttgct cttcggaaat ggcattttgc
     2881 tcgggctttt tgcggtcgcc tttattacgg tttttaatat ttgggttgag gtcggcttca
     2941 ttacctccgc cgttagaata ttttttgatt cgtgctgtac tttccaacaa ccgacgtaaa
     3001 gcatccattt ctttgtagtc gttattgttt ttgctctctt tcataatcag caaattcagc
     3061 cgtgattcca gtgtggcttc cacacgtcca acggggctga tttcgtccca tttctctcgc
     3121 tttttccaac ttgcaatggt agagacggga atatttagct gacgagaaat ttcggtcacg
     3181 gtgtaacctg cccaatactt tgattgagcc tctcgtttat cgtgtagagg gttggcagcc
     3241 aacacctcgt attcagtgat tgtattttca ttttccatag gtgcgattat caaaaatcca
     3301 ccttgtttaa gcatttgctt gaggctgtga aacccgattt aacacgtacc acacattgaa
     3361 accgccggca caaaacaaga tcataaatcc gatttttacc cctccccttt agagcaagga
     3421 aaaacagatg acaacaaaat ccaaatggtt tgtggtagca acggaaggag caactacaga
     3481 tggacgcgaa attagtcgtg aatggctcac gcagattgaa gaaaattacg acccgaaaaa
     3541 taactatggt gctcgtatta accttgagca tttccgctgg cgttggtttg agaaagatga
     3601 cccgcattct tacagctatg gcgatgtgtt agcggttaaa acagaagaac gtgaagacgg
     3661 caaattacag ttactggcag aaatcgcccc aaccgaagcc ttagtgaagt tagtcaaaga
     3721 caagcagaaa gtttatacct ctgtggaaat tgaccttaat tttgcggaca caggcgaggc
     3781 gtatttagtt ggtttggcgg tgactgatac accggcaagc ctcggcacgg aatacctcac
     3841 attttgtgcc ggtgcaaccc ataacccact agcagaccgc aagcaaaagc cggaaaacct
     3901 tgtgagtgag gcagtagagg cgaatttaga atttactgct gaacctgaaa aaggtgcgtg
     3961 gctggagaaa tttaaggcga tgtttaccaa agccaaaacc gacaatgacg gcaaatttgc
     4021 cgaacacgaa aaggcaatgg aactgttagc tgagcaattt agcaaagttt cgacagaaaa
     4081 cgaccgcttg caaactgaac taagcacatt gcaaatcgaa tttaccgaat tgcaaaaaca
     4141 agcgggcgaa tttgccgaaa aatttgcaaa actggaacaa gaaccgtctg ccaattacac
     4201 cccacgccca aaagccactg gtgcgaaagc aggtgatgta caaaccgatt tttaattgag
     4261 gatcacgaat gaaagattta acccttgaaa aatataacgc ctaccttgca cgccaagctg
     4321 aattaaacaa cttgccgttt aatgcattgg caaccggcat taaattcaca gtgcaaccat
     4381 cggtacagca aaagctgtat gaaaaggtgc gtgaaagctc tgatttctta aaatctatca
     4441 gctttgtgtt tgttgatgag caaaccggtg aaaccttagg tttagattca gctcacaccg
     4501 tggccagcac caccgatacc agcggtgacg gtgaacgcaa aaccacctct attgcgaagt
     4561 tggtaaaaca aacctaccac tgccaacaaa tcaattttga cacccatatc aattataaac
     4621 agttggatat gtgggcgaaa ttccctgatt ttcagcaaaa agtggcgaat gttgcggcaa
     4681 aacaacgcaa acgtgactta attatgatcg gctttaacgg cacaagtcgt gcggcaacca
     4741 gtgatcgcaa cagcaacccg ttattgcaag atgtggcaaa aggctggttg cagaaaatgc
     4801 gtgaagatgc caaagagcgt gtaatgaatg gcgaaagcac agacaaccaa gttttagtcg
     4861 gcaaaggtca agagtatgcc aatcttgacg cactcgtaat ggacgccacc gaagaactta
     4921 ttgatgaatg gcatcgtgat gacaccgatt tagtggtgat taccggtcgc aagttgcttg
     4981 cggataaata tttcccgatt gtgaatcagc aaaacgcacc aaccgaagaa cttgccgctg
     5041 atattgtgat ctcacaaaaa cgcattgggg gcttaaaagc ggtgcgtgtg ccgttcttcc
     5101 cggcgaatgc gattttaatc actaagctgg aaaatttagc catctatgtg caagagggga
     5161 caacccgcaa gcatattgag aacgtgccga aaaaagaccg tattgaaacc tacgaatcag
     5221 aaaatatcga ctacgttgtc gaagattatg gctgtgcggc attaatcgaa aacattacac
     5281 tcaaagataa agagtaaggt ggaactatgc gaatgagtcc ggctcgggct cattttttgc
     5341 ggaaaaccgc agaaagtgag tctgcaaccc caaaccgaga aaaccttgaa ggcttacaag
     5401 gctatgactt agttttagcc caacttaaag cccatcaacg ccaattaaag aaaatccagt
     5461 cgattgagcg aaagattgag tttaaacgca acaattttga gcaataccga ccttgggtgg
     5521 aaggctccct tgccaaaggc tccggtgtgc aagacacggt tatcaccaca atgctgattt
     5581 ggtcgattga tattggcgat tacccaaccg cccttgcgat tgctcaatac gttttaatgc
     5641 acgatttggc aatgccggag cagtttgaac gcacaccggc aaccgccttg gttgaagagc
     5701 ttgcagaggc ggcaaagaaa gcccgagacc aaaaacagcc gtttgaggca gccgtgctga
     5761 aacaggcaaa cgacctcacc gctgaatttg atatgcccga cccagtgcga gccaaattac
     5821 tgcgtgagct aggcgagcta atccaagagg cagagccaca aaccgccctt gaacactatc
     5881 aaagagcgat agccctcgac cctcaatgcg gtgcaaaagg cttgcgagac aagttagaaa
     5941 agcaacttgc caaacaagcc gaagagccga aaccagcaga agaacagaat gagtaggcga
     6001 aagccgaaat agagcgtatc cacgccagcc aaggggcgga ttttaaaggg tgatttattc
     6061 attttgcacc cttttaaaat ccccacccct taccttacgg aaaccttatg gaacaacgca
     6121 atcacgttat tagtatcccg aaagtgaatg gctatgaatc cagcaaaaat gcgatcaagc
     6181 ccaccgcaga atctgatgaa actatcacca ataatggctt ttttccgaac attgagctac
     6241 tggaggtgcg aaatgcaatg cggattgatg gcacggtcat taatgagcgg ctaaaacaag
     6301 cggtgattga ggcaatggca acagtcaatg ccgacttaaa agcctatcgc ctcaatgcag
     6361 agcaagcaca aaaagcgaac ttgcaagcct gtgatgatga gcaaatcaac ggcgaaagcg
     6421 tgctggttta caaatataaa cgagcggtct attgccttgc agtagcgaac ctttacgaac
     6481 gctaccgcag ttatgacagc accaaagacg gccacgacaa agccgaagag ctagaaagta
     6541 ccgccggtga tttgaaacga gattaccact ttgccgtgcg tgatattttg ggcgaaaacc
     6601 gaatgattag tgaattgata tgaacacctt gattgcacgc caagacgaca ccttagacga
     6661 gctgatcttt cggcactacg ggcaaacggc aggcttagtc gagcaagcct tagaatataa
     6721 ccccgaactg gctcatttgc cacgtttgcc gatcggcact gtagttacaa tgccggatat
     6781 tgagagccgt tttgcaacgg tggcgaaatc cagcgtgcaa ctttgggatt aaaaaatatg
     6841 cacaaaactg aacaagcctc ctattttggg gcaatttttg gcttttttgg tagcctcacc
     6901 ctatctgatt ggggggtggt agtgagtatt ctggtagcta ttttaacctt attacttaat
     6961 tgggcatacc gagccaaaga atacaaactg aaagagcgag aatttgagct aaagctacaa
     7021 aaccaaggga aaaagaatga gtaaaagtgt aaaagttggc gtatttgtct gctccgttgc
     7081 agctattatc gggacggtaa agcaatatta cagctccgaa atccgcacca gcgaaacagg
     7141 cttagccatt atcggcaatg cggagggttg cagacgtgat ccgtataaat gccctgccga
     7201 tgtaatcacg gttggcattg gctcaaccga ggcaagcggt gagaaaatta atgtcaatca
     7261 caaatacacc gataaagaga ttgccgagcg ttggataaaa gatcttaaaa tagctgaaag
     7321 gtgtatcaat cgttatttta atggcgacaa aatgaacgaa aatcagtttt cggcgatggt
     7381 atcagcagca tttaatatgg gatgttacaa ccttagattt tatccaaatg aaaatggtaa
     7441 atacattcag acaacaatcc ataaatatgc tatggctaag gattttaaag cgatgtgtaa
     7501 taggatacct gattttaacc gctcgggagg taaagtgcta agaggtttga aaatccgccg
     7561 agaaaaagaa aaagccctgt gtctcgggca ggaggttaaa tgattgtcaa actctgccaa
     7621 atgaccaata agcatttcag cccgctaatg attgtgctgg tgctagttgg cgtattagcc
     7681 aatttcgtgc agtttcgcca aaacgaaagc ctgaaagcag acttacaggc acgcaccacg
     7741 caaaaccaac tgattcaagc cgataaccgc aatttagcca atcagcttga aaacagccaa
     7801 atacagcttg aggcgtatca aaaacaggtg gacgacctca atcagaaagt gttagccaaa
     7861 atgaaacaag cggagcaacg aagtaatgaa atccttaacg aactggaaaa gcataaaaag
     7921 tgggctgatt ctgccgtgcc tcctagcgtt ggcaagctgc tcaaccaacg aaagcgtgcg
     7981 gtatcacacc ctcaaatcga acccgataat ctgtccgaaa aggctggagt gccaaacacc
     8041 ggcaaccgag attaacacca acggtgatct tgtcaaagca ctagacaaaa gactcaacac
     8101 cattgaggta tgccaagtca tcatcgaatc gttcgagcat tgcatcgagc aatacaacaa
     8161 aatcaaccaa ggcgagtgat cgcctttttt aataaggacg actatgtcag accaaatcga
     8221 ccgagccaac gaattagccg aaaaagccag agaggcggca cttgcaaaaa ttctgcaaaa
     8281 tcagaccgct tgcaccagcc tttttgagtg tgaggattgc ggagagccaa tccccgaaaa
     8341 acgccgagaa atggttattg gttgcacccg ttgcattgag tgccaaacca tttacgagca
     8401 taagcaaaaa ggctaccgca gatgattaaa cctgatcgct tgcgagccgt gctcacacaa
     8461 accattgata tattccaagc taaccccgaa aagctgattt tgcaatatga caaaggcaaa
     8521 attaaaagca aagggagcca aagccattcg tttgaatacc actacgattt agagctaatc
     8581 gtggtggatt ttccgtatca ccccgacgtg ttgtttgtgc cagtgctaaa ttttgtgcga
     8641 aatgaacaat gggagctact gcaaaacccg gaactacaag acaaaatcga atttgaaatc
     8701 gaccacaaca atcacgagag ctacgatatt tatatccgcc tcccgcttac cgagcgggtg
     8761 atcgtgaaag agcaagacgg acacctaact gcaacccacg ccgatgaacc gaaccttgcc
     8821 gatgtttcgc catttacccg tttaaccgaa tatgaggttt acctcaaaga cgagctgatt
     8881 tatcagtggg cagagccaaa tgagtgaacc gatcgaacaa gtaaaaaccg cctttgatag
     8941 cttactgaat aatatcagca aacctcgcag acggttgatg tatcaacaaa tcgggcgaga
     9001 gcttgcccga agccaacgca gacggattaa agcacaacaa aacccagacg gctcggcata
     9061 cgagccacgc aaaaagccga agaaaggggt aaaaagcaaa attaaatcgg gcaagatgtt
     9121 tgacaaaatt acccagccac gttttatgcg gctacgcctt gaaagcgaag gtgtcagctt
     9181 aggttatgag ggtggtgatg cggtcatcgc ccgaatccac caacaaggct taatcggacg
     9241 agtgcggaaa gattgggatt taaaagtgaa atatgccagc cgtgagttgc taggttttac
     9301 tgatgacgat ctgcagatga ttgaggatta tatgattaat attttggctg gtagctaaat
     9361 gatgaataga aacttttgca aaaaagcggt caattttgac cgcttgagtt attgattaac
     9421 gctttgtcta tctgaatgat accaacaaag aaaccctaaa atagctgaaa aaatcaatgc
     9481 tatcaaaata aaagtattga gtggtaacaa tacataaagt gagatcagaa taacgataat
     9541 agagagccca accatatttg ccaataatga aagcaggctt aaaaatactg ttgtaactac
     9601 accaaaaatg gcagcaaagg ttgtaaaacc tattgccaat aatgcaccga aaccaattaa
     9661 cccaagtaag ataaataaga tttccataga ttttccctta gccatactac aattaaggtt
     9721 ataatatgag caataattta aaaattcaag tagttctttc tgcgttagat aaattaaccg
     9781 caccatttaa gagtgtggta aaacagacgg agaaattatc tcaatcttta aaagtacaga
     9841 gagatgaatt aaaagcattg gaaaaaacac aaagtaaaat gcaatcattt aaaaaaatgg
     9901 acgatagcat taagaaatcc aatgaaacta tactcaaaca gacaaagcat ttagataatc
     9961 tcaaaaataa aatcagccaa atgaaaaatg aacgaattga gctgaaagtg aaaatggatg
    10021 cgaaaaaaag ggaatttcaa caaattacga aaggtggttc agcttggagt gcaagagccg
    10081 catttttaga taaagatttt gaaaaaatgc aaagagaata tgaaaaactc ggtgcatcta
    10141 tttcaagtgt cagccgaaaa agaacagaag aaaatcgggc attaaaggat tctcgccatc
    10201 aacaagctaa gcaactttta tattttcgta gtttaagacg tgaattgaga gcaagcggca
    10261 ttaatattaa agcttttgct caaagcgaaa ccaacttaac agacaagatt gcaaaagcaa
    10321 atcaattaat cgaccaacaa aaagcaaaat tagaaaaact aaatgcggtt aaagctcgaa
    10381 atgaaaaata ttgggcaact gtcgaaaagg taaaaaatac cagcgaacgt ttgcataatt
    10441 taggacaacg ctcaatgatt agtggtgcgg caatttctgc ccctgttgtt gggctaggga
    10501 aaggggttgt cggaatggct caaacagcgg gtaaatttga acaatttaat gctattttag
    10561 aggtaaccga gggaagtgct gaaaaagcca agcaaagttt tgattgggtt aaacaatttg
    10621 cagtagatac cccctcaaac cttgatgaag caatggaagc ctttgtaaag ttaagagcat
    10681 acggtcttga tcctaccaat ggcttactac aaacattagg tgatacaggt gctgcaatgg
    10741 gtaagcctgt aatgcaagca gttgaggcaa tagcagatgc tttaacgggt gagaatgaac
    10801 gcttaaaaga atttggtatt aaaggaagtg tagttaaagg tacaaatatt attgagtatg
    10861 cctacactga taaattaggc aaacaacaag tcgcaaaagt aaacaaaaat aaccgaaaag
    10921 aaattgagca aaccctaacc cgtattttta acgaaaaata tgctggagca atggaaaaac
    10981 aagctaaaac gctagttggt atttgggcaa aaattgaaga ttattggaca aatttccaaa
    11041 tgcaagtgat ggaatcggga gcatttgact ggattaaaaa taaattgcaa tgggtgttag
    11101 acagtttaga taaaatggct gaaaatggcg agttgcaaaa atgggctgaa gatgtcggag
    11161 ccgttattca agaaacagta caaggcttat gggcttttgg tgaaaaaatt attgaagtgg
    11221 ttaaatggtt agcgagcttg gcacgggaga ataaagggct aattgcaaca ttcgttaaat
    11281 ggtctgctat tcttggctct agtttaacgg tgctaggagc ttttgcagtg gtggcaagtt
    11341 ttgcattata tcctgtagca agattggctc ttggtatcgg caaatttaca ggtgcaagca
    11401 ccttattaaa taaggcatta tttgatagta atgataaatt ccgattattc aataaatcac
    11461 tttattcatc aggcactacg gctgcttttg ttaaatcaaa atttaatcag ttagcaactt
    11521 taccaaatgt gctgggaagt agttttaata aattaggcgg tggcatcaaa atgctaattc
    11581 cacagcttaa aacaaaagta ttttggctca atatgctaaa aggtatttta ggcaagttgt
    11641 ggagtgcaat ccgttttatt ttctcaccgt tgaaattatt acttggtatt ttctcgccga
    11701 ttggcttagc gattacagct atttcggtag caggggtagc gttatataga aattgggaga
    11761 aggtcagagc cttttttgga ggctttttgc aaggattatc taaaggtctt gcaccagtat
    11821 tagagagatt taaaccttta ggaaaactat ttgaaactat atcaaactgg gttaaatcta
    11881 cgtttaagtg gttatccgat ttattaaccc cagcggataa aacatcggaa agtttagata
    11941 aagcgagttc tgcaggagaa aaattcgggc ttgcattggc tggtgcaata gacttggtaa
    12001 catcacctgt tcagtggcta gtcgatcagt ttaagtggat ttctgaaaat atgccgagtt
    12061 gggacactat tggtgagggt gtaaaaaagg cagctactgc aattgttccc accgagcacg
    12121 ccaagcagat tactaaaacg gcagaaatgt caaacaatat gtatgacccc aattatactc
    12181 caccacaact tgagcaaaaa tggattggtg gcttggttgg taacggtaaa ggaagaggtt
    12241 ttgccacagg cggctacacc ggcaatggtg gcaaatatga gcctgccggt attgtgcata
    12301 agggcgagta tgtaatgacc aaagaggcaa ctgcacgctt gggtgtggca aatcttaacc
    12361 ggttaaatta tggcaaagtt gctgggctaa ctgcacttgc gtcaagtgtt gcgtttgctc
    12421 aaccaatgcc tgcggtcaaa atcgacagca gaccaccgct gacggcaagc caaccaagcc
    12481 caacagttgc accggtgtcg caaaatatcc atatcaccat caacgccacc ggtgggcaag
    12541 acccacaagc aatcgcccga ctggttgcaa tggagttgga aaaacaacaa cgccaagcac
    12601 aagcaagagc aagatcgtct ttgagagaca ggggctaatt tgacaaagta gaatgaaaag
    12661 cggacaatgc tctaaattgt ccgcttttta taggagcaaa tgatgtttaa agaaaaagga
    12721 tatgatgaat ttgtgaatga atgtgtgcga aaaggtagag aagaacacaa agctggttta
    12781 ggatttacgc tagagcaatc tcgcttacat atcaaaaaaa ccattgagca aaaatcacaa
    12841 gagctaaacg aatttcagcg tgaagtggtt tatggctaaa attaccatta aaccacaagc
    12901 attgagtcaa tttgacgaga ttgtaaaaag tgttatcgaa tttaccggtt ttgaaacgag
    12961 tggtattcga ttacaaagtg atatttttga caaaatagag caaattgcat ttatgccact
    13021 ctctggaaga gcaagagaag atgggacaag agaaactttc gtcagacgtt atcgtattgt
    13081 gtacgattac gataggcagg cagatgaagt aattat
//
LOCUS       DANLRK010000068        12933 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.068, whole genome shotgun sequence.
ACCESSION   DANLRK010000068 DANLRK010000000
VERSION     DANLRK010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 12933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12933
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.068"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            56..1510
                     /locus_tag="PXA33_001354"
     CDS             56..1510
                     /locus_tag="PXA33_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HDL5120683.1"
                     /translation="MKKIVSTLLILTSNLAFAETTPAQLQEKMDNQRMQKNLHTLEKP
                     EPQAKPQAVNAQAHRQSISISSQQLLQQPELLNSAMLTALVTNHTENVQFLLPIYQQL
                     PASKQNSEVVQWAEAVNAKAKQNYSEAIKGYRQLLAKYPENQPIRMQLAVALFENREN
                     EAAEAQFYKLQADKLPQEIANAVNSYLEAIGKQDQWSFQGGLTYLNDPNINNAPNAGT
                     TYGNWTAPKKESAQGVGFHFEADKKWSWGNGFFHEFRLNGNGKYYWNNKKYNEYSLRE
                     SLGLGYQNAKQKITLLPFFEQMWYAGGSSQTESTKRYSNAGGVNARWQYWITPQWQSA
                     ISYEYAEQRYTRRQHLDGNYHFIAPSLYFYPNSRQYWFVGANFNRTSPRDLDDSFIRR
                     GVSIGWGQEWKNGLSTQLSASYARKQYRAPMPIFQITQRNSEYGVQASIWHRALHFKG
                     ITPRLTWQYNKVKSNHSFYSYDKNRVYIEFSHTF"
     gene            complement(1558..4155)
                     /gene="mutS"
                     /locus_tag="PXA33_001355"
     CDS             complement(1558..4155)
                     /gene="mutS"
                     /locus_tag="PXA33_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDL5120684.1"
                     /translation="MTQDFSTHTPMMQQYLQLKAQNPDILLFYRMGDFYELFYDDAKR
                     AAALLDISLTKRGQSAGEPIPMAGVPYHAVEGYLAKLVALGESVAICEQIGDPATSKG
                     PVERKVVRIVTPGTVSDEALLPERQDNLVAAVYEEKGTFAIATLDMTSGRFLISELAS
                     KEALSAELQRVQPAEILYAEDFSAASILTNYKGLRRRPVWEFELVTAIQLLNRQFGTQ
                     TLAGFGVEKAVVALCAAGCVLHYAQETQRTALPHINAIHLAQHSDTILLDAATRRNLE
                     LTQNLAGGTENTLAAVLDKCVTPMGSRLLKRWIHQPIRDCGKLQARQKAITALIQYER
                     MSDLQPLLQQVGDMERILARVALRTARPRDLTRLRTALAQLPEIAKNSQNLTASIDQL
                     LAQLADFSPLHDLLERAIIENPPQLIRDGGVIAEGYNAELDEWRQLSDGATQYLENLE
                     IRERESTGIDSLKIGFNAVHGYYIQISQGQAHKAPIHYVRRQTLKNAERYIIPELKTY
                     EDKVLKAKGASLALEKQLYDELFDVLMPRLGELQLAAMMLSELDVLTNLAERADSLGY
                     VCPSFSPMRMVNIKGGRHPVVEQVLTEPFIANPVFLNSQRHLLIVTGPNMGGKSTYMR
                     QIALITLMAYIGSFVPAESAEIGPIDRIFTRIGASDDLASGRSTFMVEMTEMANILHQ
                     ATENSLVLIDEIGRGTSTYDGLSLAWACAEWLAKKTQSLTLFATHYFELTGLPNQLKG
                     VANVHLDAREHQDSIVFMHSVQEGAASKSYGLAVAALAGVPKQVIQLAKQRLAHLEEI
                     SHQTKQANEHPQADLLFSDGFDLQKNEAIQPLVVENEVEKALKALQPDELTPRQALDE
                     LYRLKKLLG"
     gene            4305..4868
                     /locus_tag="PXA33_001356"
     CDS             4305..4868
                     /locus_tag="PXA33_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005613140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5358 domain-containing protein"
                     /protein_id="HDL5120685.1"
                     /translation="MLRKIVLLSTGLLLAACSSNEPQQPEAFPGEFANADYVLSEENA
                     QKWVIASEQAKQCIYPNLTRIQQEHFSKEDSYIFSQYVFFYPLEEIIGEQYVKIIQND
                     EKSMGYAQYLFKKLKNNEAVEALPKKQCATLRTNAKNDLAVVKGQYQSGMIEETNSQE
                     GKSGDKVATNDNKFFFDIIKWGSMLLL"
     gene            5042..5671
                     /locus_tag="PXA33_001357"
     CDS             5042..5671
                     /locus_tag="PXA33_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HDL5120686.1"
                     /translation="MAVSATSFAQTTQPTAAEQMNAVKASVAEKATVAKEATKEKAAE
                     LKAGSAEKMAIAKAKTESFKDSAKNKMDAVKATTAEKTDVIKAKAASASDTMKVKAQE
                     AKAATAEKAATAHETLNSAKTATAEKAASMKAAATEKMSAVKKSQKVNINTANEEQLQ
                     SLTGIGEAKAKAIVEYRKKHGKIKNAAELANIPGIGDATIEKVAPYLTF"
     gene            5819..6448
                     /locus_tag="PXA33_001358"
     CDS             5819..6448
                     /locus_tag="PXA33_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007526112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDL5120687.1"
                     /translation="MALDFNSVGLDSVEAKGGYGIGLQIGQQLLGSGMDVNAETVARG
                     IYDVLNNNEPAIDINEVTAALQELGQRAEAAQAEAFKAIDAENKAFLEENKKANGVIV
                     TDSGLQYEILTEGTGEKPTATSTVRVHYTGSLIDGTVFDSSVKRGQPAEFPVNGVIRG
                     WTEALQLMPVGSKWRLTIPQELAYGERGAEASIPPFATLVFEVELLDIL"
     gene            6514..7029
                     /locus_tag="PXA33_001359"
     CDS             6514..7029
                     /locus_tag="PXA33_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein disulfide oxidoreductase"
                     /protein_id="HDL5120688.1"
                     /translation="MQKKSLISRLLKNIFLYGSLFIIISLVVDWYRSPSAPAQFEQTV
                     HYDIQHQPKVIAQLSHEKPMLLYFWGSWCHYCQVVSPNIQQLANDGTEVLGVALKSGD
                     ENDVQAYLNENGYTFATLNDPSGDFSRGWNIQATPTILIIKDGKIAHHTTGYTSYLSL
                     KFRLWLADVLY"
     gene            complement(7077..8007)
                     /locus_tag="PXA33_001360"
                     /pseudo
     CDS             complement(7077..8007)
                     /locus_tag="PXA33_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433453.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(8066..8716)
                     /locus_tag="PXA33_001361"
     CDS             complement(8066..8716)
                     /locus_tag="PXA33_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595-like element ISMha4 family transposase"
                     /protein_id="HDL5120689.1"
                     /translation="MRKSRLSQYKQNKLIEQFVAGVTARTASELVNVNKNTAAYYFHR
                     LRLLIYQHSPHLEMFEGEIEVDESYFGGHRKGKRGRGAAGKTAVFGLLKRDGKVYTVV
                     VPNTQFATLLPIIREKVKPDSIVYTDFYRSYDVLDVSEFNHFRINHSTHFAENQNHIN
                     GIENFGSQAKRHLRKFNGIPKVHFELYLKECEWRFNHSDLKSQISFLKQLVKGSLG"
     gene            9045..10403
                     /gene="mnmE"
                     /locus_tag="PXA33_001362"
     CDS             9045..10403
                     /gene="mnmE"
                     /locus_tag="PXA33_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007523948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HDL5120690.1"
                     /translation="MKDTIVAQATPIGRGGVGILRVSGPLAETVAQAVLGKTLPPRIA
                     NYLPFKDEDGTVLDQGIALFFKAPNSFTGEDVLELQGHGGQVILDLLLKRILQVKGLR
                     IARAGEFSEQAFLNDKLDLAQAEAIADLIDATSEQAARSALKSLQGEFSNKINALVDS
                     VIYLRTYVEAAIDFPDEEIDFLADGKIEAKLNEIIAQLDGVRREAKQGSILREGMKVV
                     IAGRPNAGKSSLLNALAGREAAIVTNIAGTTRDVLREHIHIDGMPLHIIDTAGLRDAS
                     DEVERIGIQRAWEEIAQADHVLLMIDSTEQQADQFRQEWAEFLAKLPANMPVTVIRNK
                     VDLSGEQEGLIQVDDFTMIRLSAQTKVGVDLLREHLKKSMGYQSSTEGGFLARRRHLQ
                     ALETAAEHLERGHIQLTQFLAGELLAEELRMVQNALSEITGQFTSDDLLGNIFSSFCI
                     GK"
     gene            10404..11669
                     /gene="tilS"
                     /locus_tag="PXA33_001363"
     CDS             10404..11669
                     /gene="tilS"
                     /locus_tag="PXA33_001363"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HDL5120691.1"
                     /translation="MALFNRFQTQCATHLPNQTAFLIGLSGGVDSVVLLHLFARTSFH
                     VRAIYVHHGLSPNADDWAAFCEQYCKRLNIPFILQKVRVDSSNGVENGAREARYQAIQ
                     QQLKPNEVFATAHHLDDQAETFLLALKRGSGVKGLSAMQAVTFLQNFTVFRPLLTFTK
                     SDLMGYAVQHQLGWIEDESNADNRYDRNFLRNSILPLLNQRWQHFSQMVARSAQHCAE
                     QQALIEELLSNELKSRTGEKQQLNINGFGQFSLAKQQQLSRLWLEQNGVRMPSQAQLQ
                     AVISELIFAKADKNPQVKVGENIIRRYQQAIYVTEEIADIPPFEVVLPTETKVELPYQ
                     LGTIIRQQQEIICKKNGKNHRLQLPQELAQSTISIKIGQLGKVKCYGKAQREEMKKIW
                     QNHGIPVWERNRTPLIFWQDELVGLLPIL"
     gene            complement(11659..12552)
                     /gene="xerD"
                     /locus_tag="PXA33_001364"
     CDS             complement(11659..12552)
                     /gene="xerD"
                     /locus_tag="PXA33_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HDL5120692.1"
                     /translation="MAELDPIIEQFLDTLWQEHGLSENTVASYRSDLERFSDYFSEPK
                     AFLSLDHIDLQGFLGERLEQGYKATSSARMLSCLRKFFRFLCLEHYRQDDPTLTLSSP
                     KRGASLPKSLSEDQVMDLLDAPNTLDPIELRDKAMLELLYATGLRVTELVSLSMDNIS
                     LKQGVVRIIGKGDKERLVPLGEEANYWIQEFFQYGRPILLNNQQSDVVFPSRRGQQMT
                     RQTFWHRIKHYAVLAGIDADKLSPHVLRHAFATHLVNHGADLRVVQMLLGHSDLSTTQ
                     IYTQVAKARLKSLHQQFHPRG"
     gene            complement(12669..12745)
                     /locus_tag="PXA33_001365"
     tRNA            complement(12669..12745)
                     /locus_tag="PXA33_001365"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12709..12711),aa:Asp,seq:gtc)
     gene            complement(12756..12871)
                     /gene="rrf"
                     /locus_tag="PXA33_001366"
     rRNA            complement(12756..12871)
                     /gene="rrf"
                     /locus_tag="PXA33_001366"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         3874 a         3009 c         2742 g         3308 t
ORIGIN      
        1 cttcactaaa ggctattgaa aaatagcctt ttctattcaa ataaaagata gctttatgaa
       61 aaaaatagtc tcaacgcttt taatccttac ctcaaacctg gcatttgcgg aaaccacacc
      121 ggctcaactt caagaaaaaa tggataacca gcgaatgcag aaaaacctac atacattaga
      181 aaagcctgaa cctcaagcca aaccacaagc ggttaatgca caggctcacc ggcaatcgat
      241 ctctatttca agccaacagc tgcttcaaca gcctgagttg ctcaactcgg caatgctaac
      301 tgcgttggtc actaaccaca ctgagaatgt tcaattctta ctgcctattt atcaacaatt
      361 acccgccagt aaacaaaaca gcgaagttgt acaatgggcg gaggcagtta atgccaaagc
      421 aaaacaaaat tattcagagg caataaaagg ctaccgtcag ctactggcaa aataccctga
      481 aaatcagcca atccgtatgc aattagcggt tgcattgttt gaaaatcggg aaaatgaagc
      541 cgcagaggct caattttata agttacaagc ggacaaatta ccgcaagaaa ttgcaaatgc
      601 ggtcaatagc tatttggagg cgattggcaa gcaagatcaa tggagttttc aaggcggctt
      661 aacctatttg aatgacccga atatcaacaa tgctccaaat gcaggcacga cctatggcaa
      721 ctggactgca ccgaaaaaag aatccgccca aggggtcggc tttcactttg aggcggataa
      781 aaagtggtct tggggcaatg gtttcttcca cgagtttcgt ttaaacggaa acggcaaata
      841 ttactggaat aacaaaaaat ataacgaata ttcgctcaga gaaagcctcg gcttgggtta
      901 tcaaaatgca aaacaaaaaa tcaccttgtt gccgtttttt gagcagatgt ggtatgccgg
      961 tggctcaagc caaaccgaaa gcaccaaacg ctactccaac gcaggcggtg tgaatgcccg
     1021 ctggcaatat tggattactc cacagtggca atctgcaatc agctacgaat atgcggaaca
     1081 gcgttacact cgccgccaac atttagacgg caattatcat tttattgccc cgagcctcta
     1141 tttttaccct aacagccggc aatattggtt tgtgggggcg aattttaacc gcaccagccc
     1201 ccgtgattta gatgatagct ttattcgccg aggtgtttct attggttggg gacaagagtg
     1261 gaaaaacggg ctttcaaccc aattaagtgc cagctacgcc cgcaaacaat accgagcccc
     1321 aatgccgatt ttccaaatta cacaacgtaa cagtgaatat ggtgtgcaag ccagcatctg
     1381 gcacagagcc ttgcatttta agggtattac ccctcgctta acgtggcaat ataacaaggt
     1441 aaaaagcaat catagcttct atagttatga caaaaaccgt gtctatattg agttttcaca
     1501 tacgttctaa acaaaaacgc cccttaaacg gggcgatttc ttagttattt tttcaattta
     1561 ccccaacaat tttttcaagc gatacaactc gtccaaagcc tgtcttgggg taagttcatc
     1621 aggctgaagg gctttcaggg ctttttcaac ttcattttct actacaagcg gttgaattgc
     1681 ttcatttttt tgcaaatcaa aaccatcgga aaacaacaaa tccgcctgtg gatgttcgtt
     1741 tgcttgtttg gtttggtggg aaatttcctc caaatgtgcc aaacgctgct ttgccagttg
     1801 aatcacttgc tttggcacac ccgccaaggc tgccaccgcc aagccgtaac ttttgctggc
     1861 ggccccctct tgcaccgagt gcataaacac aatactgtct tgatgttcac gcgcgtctaa
     1921 atgcacattt gccacccctt tgagctgatt cggcaagccg gtaagttcaa aatagtgggt
     1981 tgcaaacaaa gtgagtgact gggttttctt cgccagccat tcggcacacg cccacgccag
     2041 tgataagccg tcataggtag aagtgcctcg cccgatttca tcaatcagca caaggctgtt
     2101 ttcggtggct tgatgcagaa tattcgccat ttcggtcatt tccaccataa aggttgaacg
     2161 ccctgaagct aaatcatcgc ttgccccaat tcgggtgaaa atacggtcaa tcgggccgat
     2221 ttccgcactt tctgccggca caaaactgcc gatatacgcc atcaatgtaa tgagggcaat
     2281 ttgccgcata taggtgcttt taccgcccat attcgggccg gtcacaatca ataaatgccg
     2341 ttgggaattt aaaaacaccg gattggcaat aaacggctcg gttaaaacct gctccaccac
     2401 cgggtgtctg ccgcctttaa tattcaccat tcgcatcggg ctaaagctcg ggcaaacata
     2461 gcctaaacta tcagctcgct cggcaaggtt ggttaagaca tcaagttcag acagcatcat
     2521 cgccgctaat tgcaattcgc ctaagcgagg cattaacaca tcaaacagtt catcataaag
     2581 ctgtttttca agggcaagcg atgccccttt tgctttcagc actttgtctt cgtaagtttt
     2641 cagctcaggg ataatatacc gctccgcatt tttgagcgtt tggcggcgaa catagtgaat
     2701 cggggctttg tgtgcttgcc cttggctgat ctgaatgtaa tagccgtgaa ccgcattaaa
     2761 gccgattttc agcgaatcaa tgccggtgct ttcacgttct ctgatctcca aattttccag
     2821 atattgcgtt gccccgtccg aaagttgccg ccattcgtca agctcggcat tataaccttc
     2881 tgcaatcacc ccaccatcac gaatgagttg tggtgggttt tcgataatcg cccgctccag
     2941 caaatcgtgt aacgggctga aatccgccag ttgagccaaa agctgatcga tactagcggt
     3001 cagattttgt gaattttttg caatttccgg taactgagcc aatgcagtgc gtagtcgggt
     3061 gaggtcacga gggcgagccg tacgcaacgc cactcgggcg agaatccgct ccatatcgcc
     3121 gacttgttgt aataacggct gtaaatccga cattcgctca tattgtatca atgctgttat
     3181 cgccttttgt cgggcttgta atttaccaca atctcgaatc ggctgatgaa tccaacgctt
     3241 gagcagacgg ctccccattg gggtcacaca tttatctaac accgccgcca gcgtgttttc
     3301 cgtgccaccc gctaagtttt gggtcagctc taaattgcga cgagtagctg catcgagcaa
     3361 aatagtatcg ctatgttgtg ccaaatgaat tgcgttaatg tgcggtaaag cggtgcgttg
     3421 cgtttcttgg gcataatgca agacacagcc ggcagcacaa agtgccacca ccgctttttc
     3481 caccccaaat ccggcaagtg tttgcgtgcc gaattggcga tttagcagct gaatcgccgt
     3541 taccagctca aattcccaca ccggacggcg gcgtaaccct ttgtaattcg ttaaaattga
     3601 ggcagcggaa aaatcttccg catacagaat ttctgccggt tgtacacgtt gtaactcggc
     3661 ggatagagcc tctttgctag caagttcact gattaaaaac cgcccggagg tcatatctag
     3721 cgtagcaatc gcaaatgtgc ctttttcttc gtaaactgcc gccaccaagt tatcttggcg
     3781 ttcgggtaac aatgcctcat cgctaactgt tcccggcgta acaatccgta ccactttgcg
     3841 ttctaccggc ccttttgagg ttgctgggtc gccaatctgt tcacaaattg ctaccgattc
     3901 ccccaacgcc accaatttcg ccaaataccc ctccactgcg tgataaggca cacccgccat
     3961 cggaatcggc tcaccggcag attgccctcg cttggtgagc gaaatatcca acaatgctgc
     4021 cgctcgtttc gcatcatcat aaaacagctc ataaaaatcc cccattcggt aaaacaatag
     4081 tatgtccgga ttttgagctt tgagttgcaa atattgttgc atcattggcg tatgggtgga
     4141 gaaatcttgt gtcatcagtc tatttattgt gagaaatcat tgctgatagt ttaacagatt
     4201 cagccaaggg ataaaaacaa gcggtcattt ttcgctgaaa atttgcaatg gtcagccgct
     4261 gtttttttgt tacaatgcac caaattttta aaacagaggt cagaatgtta agaaaaatag
     4321 tgctactttc tacaggctta ttattggcgg cttgtagcag caatgaaccg caacaaccgg
     4381 aagcattccc gggcgaattt gccaatgccg attatgtgct ttcggaagaa aatgctcaaa
     4441 aatgggtaat tgccagcgaa caggcaaaac agtgtatcta ccccaattta acccgtattc
     4501 agcaagagca tttttccaaa gaagattctt atattttctc acaatatgta tttttctatc
     4561 cgcttgaaga gattatcggg gagcagtatg taaaaatcat tcaaaatgat gaaaaatcaa
     4621 tgggttacgc tcaatattta ttcaaaaaac tgaaaaataa cgaagcggtt gaagcgttac
     4681 cgaaaaaaca atgtgcgacc ttacgcacca atgcaaaaaa tgatttggca gtggtaaaag
     4741 ggcagtatca aagcggtatg atcgaagaaa ccaatagcca agaagggaaa tcaggcgata
     4801 aagttgccac caatgataat aaattcttct tcgatattat caaatggggc agtatgttac
     4861 ttctctagtg agcctcaata ctcggttgtt ttaaccacaa ccgagccatt tatacctacc
     4921 gattgaactt ttatctaaag cctcagtctt aaaatacagt ttttttcaat tattttccct
     4981 cttttatatt taaggatttt caataaaagc attaaaaaca ttattagcat tgggcgtggc
     5041 aatggctgtt tcagctactt catttgcaca aaccactcaa cctactgcgg ctgaacaaat
     5101 gaacgcagtt aaagcctctg ttgccgaaaa agcaactgtg gctaaagaag caacaaaaga
     5161 gaaagcagcg gagttaaaag ccggttctgc ggaaaaaatg gcgatagcaa aagccaaaac
     5221 cgaatcattt aaagattccg ctaaaaacaa aatggacgca gtaaaagcca ccacagcaga
     5281 aaaaacggac gtgattaaag cgaaagccgc atctgcaagc gacacaatga aagtgaaagc
     5341 tcaggaagca aaagccgcca ccgcagagaa agctgcaact gctcatgaaa ccttaaattc
     5401 agccaaaacc gcaaccgctg aaaaagcggc ctcaatgaaa gcagctgcaa cagaaaaaat
     5461 gtcagcagtg aaaaaatcac aaaaagtgaa tatcaacact gccaacgaag agcaattaca
     5521 atcgcttacc ggcattggtg aagcaaaagc caaagcgatt gtagaatacc gcaaaaaaca
     5581 cggtaaaatc aaaaatgcag cagaactggc gaacattccc ggcattggcg atgcaaccat
     5641 tgagaaagtt gctccatatt taactttcta atgttctgca agcggttatt ttgttgcaaa
     5701 attttgcaga aaaataaccg cttgttcatt tcacaccttg tattcttaaa tttcaccccc
     5761 attaagtgaa tcgtatttct ttcaatacag tattcaataa actcaaaagg aacagaaaat
     5821 ggcattagat ttcaattcag ttggattaga ttcagtagaa gcaaaaggcg gttacggcat
     5881 cggtttacaa attggtcagc aacttttagg cagcggaatg gacgtaaacg cagaaaccgt
     5941 tgcccgtggt atttatgatg tgttaaacaa caatgagccg gcaatcgaca tcaatgaagt
     6001 gaccgctgca ttacaagaat taggtcagcg tgcggaagcg gctcaagcag aagcattcaa
     6061 agcgattgat gccgaaaaca aagcgttctt ggaagagaat aaaaaagcaa acggtgtaat
     6121 cgttaccgac agcggtttac aatatgaaat tttaaccgaa ggcacaggcg aaaaaccgac
     6181 tgcgacttca accgtgcgtg ttcactacac cggttcatta attgacggca ccgtgtttga
     6241 cagctcggta aaacgcggtc agccggcaga attcccggta aacggagtaa ttcgtggttg
     6301 gacagaagca ttgcaattaa tgccggttgg ctcaaaatgg cgtttaacca ttccacaaga
     6361 attagcctac ggcgaacgtg gtgcagaagc atcaattcca ccatttgcga ccttagtgtt
     6421 tgaagtggaa ttgcttgata ttctctaatc aaactcaaat aaaaaacctg tggaggcatt
     6481 tcacaggttt ttgctattta agataactat attatgcaga aaaaatcact catatctcgc
     6541 ttacttaaaa atattttcct ctacggcagc ctgtttatca ttattagcct ggtggttgat
     6601 tggtatcgct cgccctccgc tccggcacaa tttgagcaaa ccgtgcatta tgatattcaa
     6661 caccagccga aagtaatcgc ccaattaagc cacgagaagc cgatgctact ctatttttgg
     6721 ggaagttggt gccactattg ccaagtggtt tcgccaaata tccaacaatt agcgaatgac
     6781 ggcacggaag ttttaggtgt tgccctgaaa tccggcgatg aaaatgacgt acaagcctat
     6841 ctcaatgaaa atggctacac tttcgctact ttgaatgacc caagcggtga tttctctcga
     6901 ggttggaaca ttcaagccac cccaaccatc ttgattatca aagacggtaa aatcgcccac
     6961 cacaccaccg gctacactag ctatttgagc ttaaaattca gactatggct ggcagacgtg
     7021 ttgtattaaa cgattccttt catttgcaaa aaaatccact aaatccgacc gcttgtttac
     7081 accaaaaaga caagcggtac taaattcatt gtcatcattg tggtgatcga taaaatcagc
     7141 ctcgatgagc caaaaataaa gcctcggttt ttgccgttgt cgaatttcac cagtaaattt
     7201 gccattgctg tgagggcgta cacttccacc accgtaaaaa tcacggtgta aatataggca
     7261 gcggtcagag ttgcgacttt taccgcaata aaccaagggg caataatcgc caccgctaat
     7321 aacggcgaaa tccaaattaa ttgctgtttg gtgaggttga ttttacgaga aaaagtcgat
     7381 tgcaaactga cggttaaaac accgttgatg aataacgcaa tcggcgaatg ctccggtgcg
     7441 tgcaggacat tgtcgaataa atacggaaaa atcactcagc ggcacaaata gcattaagtg
     7501 agcgaacaaa aactctttgt tgatgatttc cttaatttgt gcgaaacgaa atttcacgcc
     7561 gccattcacg ccttccaccg aataaaacgg cttgaccata aacgcaaaca agccggcttc
     7621 caaaataaag cagatccaaa tcagcgtttc aatcatctca aacctaataa acggaataat
     7681 aatcagcggg gcgagcattg aagacatact ggtgactttt agatatttgc cttgtaaacg
     7741 ggttttagca gcatattcat cgccggcaat cgccagtaaa caggctctgg cattagtgcc
     7801 gaataagcaa ctccccaacc caaagcaagt ggttgccaac aataaaatga ggtaattatc
     7861 cgctgctaaa aacagcatat aggcaaccac atctaataaa cagcccaata acatcatttt
     7921 tgccaagccg tagcgatccc cccaaatgcc ggctaaaatt gccaatgcct gattacaaaa
     7981 caacagcaat gaaaggaaaa aagcgatttg ggcgttatct agccctttct cttttaaata
     8041 aaagaaaagg ggctgtccta gataactaac ctaaactacc cttaaccaat tgttttaaaa
     8101 aggatatttg agattttaag tcactgtgat taaaacgcca ttcgcattct ttcaaataca
     8161 gctcaaaatg cactttggga atgccgttaa acttacgcaa atggcgtttt gcttggctcc
     8221 caaaattctc aattccatta atgtgatttt gattttcagc gaagtgtgtg ctgtgattaa
     8281 tgcgaaaatg gttaaattca ctcacatcaa gcacatcata acttcgatag aaatcggtgt
     8341 aaacaatact atcgggtttc accttttccc gaataatcgg caaaagtgtg gcaaattgcg
     8401 tgtttggtac taccacggta taaacctttc cgtcccgctt taaaagcccg aacacggcgg
     8461 ttttgcctgc tgcaccacgt ccacgtttac ctttacgatg accgccgaaa taactctcat
     8521 caacctcaat ttctccctca aacatttcca aatgagggct atgttgatag ataagcaacc
     8581 gcaagcgatg gaagtaatac gccgccgtgt ttttgtttac attgaccaac tcagatgctg
     8641 ttcgggctgt tacccctgca acaaactgct caatgagttt gttttgtttg tactgactta
     8701 gacgactttt tctcatttgg gtatcttaac ctaaatggag tttttagtcg ttatctagga
     8761 cagccccaaa gaaaaacacg ctttgcattg aaaaaaaggc ggtggtggaa ataaaacggc
     8821 gtaggagtaa tagtttttct aaagacattc agaatggttg aattggttaa ataaagagcg
     8881 gttattctac cttttaagtc aaccccaatc aatcggcttt tgttttacct ttggcgtggg
     8941 tttcggtatg ataagcgata tgtagtggcg ggtcctgtgc ccgcctaaat accaatgata
     9001 acttgcgggc gagcacagga ctcgccccta cgataaacaa gaaaatgaaa gacaccattg
     9061 ttgctcaagc aacaccgatt ggcagaggcg gtgtggggat tctgcgagtt tccggtccgc
     9121 ttgccgaaac cgttgctcaa gccgtattag gcaaaacatt gccaccccgt atcgcaaatt
     9181 atttgccgtt taaagacgaa gatggcactg tgttggatca aggcatcgcc ttatttttta
     9241 aagctcccaa ctcctttacc ggcgaagatg tgcttgaact tcaaggacac ggtgggcagg
     9301 ttattttaga tttactgctc aaacgcattt tacaagttaa ggggctacgc attgcccgag
     9361 caggtgaatt ttccgaacag gcatttttaa acgataagct cgaccttgcc caagctgaag
     9421 cgattgcgga tttaatcgac gccaccagcg agcaagccgc tcgttcagcc ctgaaatcac
     9481 tacagggtga attttctaat aaaatcaatg cgttagttga ttcagtaatt tatttacgca
     9541 cttatgtgga agcggcgatt gacttccccg atgaggaaat tgactttctg gcagacggta
     9601 aaatcgaggc aaaattaaat gagattatcg ctcaattaga cggtgttcgc cgtgaagcga
     9661 aacaaggctc gattctgcgt gaaggaatga aagtagtgat cgccggtcgc ccgaatgcag
     9721 ggaaatcaag cttgcttaac gccttagccg gacgagaggc ggcgattgta accaatattg
     9781 ctggcacaac ccgtgatgtg ttgcgtgaac atattcacat tgacggaatg ccgttgcata
     9841 ttatcgacac cgccggtctc cgtgatgcca gtgatgaggt ggaacgtatc ggcattcagc
     9901 gtgcttggga agaaatcgcc caagccgacc acgttttatt aatgattgac agcaccgagc
     9961 aacaagccga tcagttccga caagagtggg cggaatttct agccaaactg ccggcaaata
    10021 tgcctgtgac cgtaattcgc aacaaagtcg atttatccgg cgagcaggaa ggcttaattc
    10081 aggtggacga tttcacgatg attcgcctct ctgcccaaac caaagtcggc gttgatttat
    10141 tgcgtgaaca cttgaagaaa tcaatgggct accaaagttc tacggaaggc ggattcttgg
    10201 ctcgccgccg tcatctgcaa gcattggaaa ccgctgccga gcatttagag cgagggcata
    10261 tccagcttac tcaattctta gccggcgaat tgttagcgga agagttgcgt atggtgcaaa
    10321 atgccttaag tgaaattacc ggccagttca cctctgatga cttattaggc aacattttca
    10381 gctcattttg catcggtaaa taaatggcac tttttaaccg ctttcaaacc cagtgtgcaa
    10441 cgcatttgcc gaaccaaacc gcctttttaa tcggcttaag cggtggggtg gattcggtgg
    10501 tactactcca cctttttgcc cgcacctcat ttcacgttag ggcaatttat gtccaccacg
    10561 ggctaagccc aaatgcggac gactgggcag ccttttgcga acaatattgc aagcggttaa
    10621 atattccctt tattttacaa aaggtcagag tggatagctc aaacggcgtt gaaaacgggg
    10681 cgagagaagc tcgttaccaa gccattcaac aacagcttaa gcccaatgaa gtgttcgcca
    10741 ccgcccacca tttagatgac caagccgaaa cttttttgct cgccctcaag cgaggcagtg
    10801 gagtcaaagg attatcggcg atgcaagcgg tcactttttt gcaaaatttt accgtttttc
    10861 gaccgctact cacttttact aagtcagatt tgatgggtta tgccgttcaa catcagctag
    10921 gttggattga ggacgaaagc aatgccgata accgttacga ccgtaatttc cttcgcaata
    10981 gcatcttgcc actactcaat caacgttggc agcacttcag ccaaatggtt gcccgttccg
    11041 ctcagcattg tgcagaacag caagccttga tagaagagct attaagcaat gaactcaagt
    11101 caagaacagg ggaaaaacaa caactcaaca ttaacggctt tgggcaattt tcacttgcca
    11161 agcaacagca acttagccgc ctttggctgg aacaaaacgg agtgagaatg ccaagccaag
    11221 cccagctaca agcggtcatt tctgaactga tttttgcaaa agccgataaa aacccacagg
    11281 ttaaagtcgg cgaaaatatt atcagacgct atcaacaagc tatttacgtt accgaagaga
    11341 tcgccgacat tccgccgttt gaggtagtac ttcccacaga aaccaaagtg gaattaccct
    11401 atcagctcgg tactattatt cgccaacaac aagagatcat ttgcaaaaaa aacgggaaaa
    11461 atcaccgctt gcaactgccc caagagctgg ctcaatcaac tatttctatt aaaatcgggc
    11521 aactaggcaa agtaaaatgc tacggcaagg ctcaacggga agaaatgaaa aagatttggc
    11581 aaaatcacgg catacctgtt tgggaacgca accgtacacc gctgattttt tggcaagacg
    11641 aattagttgg cctattacct atcctctagg gtgaaattgc tgatgaagcg atttcaaacg
    11701 ggcttttgcc acttgagtgt aaatctgggt ggtcgataaa tcactatgcc ccagcaacat
    11761 ctgtaccact cgcaaatcag ccccatgatt gaccaaatgg gtcgcaaagg cgtggcgaag
    11821 cacgtgaggc gagagtttat ctgcatcaat acctgcaagc acggcatagt gcttaatccg
    11881 atgccaaaac gtctgccttg tcatctgctg acctcgccgg ctcggaaata caacatcaga
    11941 ctgctgatta ttcagcaaaa tagggcgacc gtactggaaa aactcctgta tccaataatt
    12001 cgcctcctcg cccaatggca ctaaccgctc tttatcccct ttaccgataa ttctcaccac
    12061 cccttgtttg aggctgatat tatccatcga aagcgacacc agctccgtta cccgcagacc
    12121 ggtggcatac agcagctcca gcattgcttt atcccgtaac tctatcggat ctaacgtatt
    12181 cggggcatct agcaaatcca ttacctgatc ttcacttaag gatttgggta aagaagcacc
    12241 ccgtttaggg gaagaaaggg ttaaagtcgg gtcatcttgg cgataatgct caaggcataa
    12301 aaaacggaaa aatttcctta aacaactcaa cattctggca gagcttgtcg ccttatagcc
    12361 ttgctccaaa cgctcgccga gaaaaccttg taagtcgata tgatcgagag acagaaatgc
    12421 tttaggttca gaaaaataat cggaaaaacg ctctaagtca gaacgataag aggcaacggt
    12481 attttcagac aaaccgtgtt cttgccatag tgtatcgaga aattgttcaa taatagggtc
    12541 aagctcagcc atacaagcgg gtatattttc aggaaatttt gcaaatttta acagaattaa
    12601 atctataaaa atacaaaagc gatagaaacg aaaaaaccga ttagcttatg ctaatcggtt
    12661 taaattattg gcggaacgga cgggactcga acccgcgacc ccctgcgtga caggcaggta
    12721 ttctaaccag ctgaactacc gctcctaaat taatagattg ataatgccct actctcacat
    12781 ggcgaatcac cacactacca tcggcgttac tgcgttttac ttctgagttc ggtatggagt
    12841 caggtagagc cacagcactc tggttatcaa tcaaattctt tcatttagtc agtttactaa
    12901 aatatagttt atactttcta ataaactcac taa
//
LOCUS       DANLRK010000069        12926 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.069, whole genome shotgun sequence.
ACCESSION   DANLRK010000069 DANLRK010000000
VERSION     DANLRK010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 12926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12926
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.069"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            265..1182
                     /locus_tag="PXA33_001367"
                     /pseudo
     CDS             265..1182
                     /locus_tag="PXA33_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014167835.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(1184..1852)
                     /locus_tag="PXA33_001368"
     CDS             complement(1184..1852)
                     /locus_tag="PXA33_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HDL5120693.1"
                     /translation="MKYLYALIFSLFFSSVYAAERQLQCKVVGISDGDTLTCLVNKTP
                     LKIRLQHIDAPEQAQPYGNKAKQALAQFVFQQNITAIVSGYDRYQRLLATLYLNDKNI
                     NLALVENGMAWAYTTKREYIEAQDQAQQAKIGLWQDKFPINPSQWRKTHTSTLPQAVK
                     KPENIANQPPTIDCQIKLSCNQIGNYAQAKRYFQQCGWKELDGNNDSIPCNKLYRQEQ
                     KNRN"
     gene            complement(1896..3263)
                     /locus_tag="PXA33_001369"
     CDS             complement(1896..3263)
                     /locus_tag="PXA33_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HDL5120694.1"
                     /translation="MSIDTILNQINSFVWGPPLLILISGVGLYLTFSLKGIQLSQLGQ
                     AFRYMFKPEKGKGQSGDISAFAALSTALAATIGTGNIVGVATAIHSGGPGALFWMWLI
                     ALFGMATKYAEGLLAIKYRTKDRAGFVAGGPMYYIELGMGKKWRWLAKAFAFFGVLVA
                     LLGIGTFPQVNGITHALESTFDVPIIFSAIILTLIVTAIILGGVQRISKIATIVVPFM
                     AIGYVIAALSVILLNMEKVPQAIITIVHAAFNPQAALGGAVGFTVMQAIQLGVARGIF
                     SNESGLGSAPIAAAAAQTKEPVRQGLISMTGTFLDTIIVCTMTGLVIVISGAWAGDVK
                     GAELTTTAFTQGLNSSFGAIIVTVGLVFFAFTTILGWCYYGERCFVYLTRGRTQYIKL
                     YRLLFIALIASAPFLQLDTIWTLADIVNGLMAFPNLIALIALRKVIISETKDFFARLK
                     TGKAE"
     regulatory      complement(3382..3477)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(3648..4700)
                     /locus_tag="PXA33_001370"
     CDS             complement(3648..4700)
                     /locus_tag="PXA33_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL5120695.1"
                     /translation="MTHSTKTLVAIASIIIILAGIKLSNEIVVPFLLSLFIAIICSPM
                     IKFMTKRKVPLWLAITILLIVFILGFSFLAGIINNTLQEFTNAIPQYKIQLSSRIQVI
                     LELAHRWHIPLTLSQEEIMSKFDPSSIMNLVSKLLMGLSGALSNIFVIFLVVIFMLLE
                     MPVAKYKLALALSPDDDLSNEKEYINQVLEGVIGYLGVKTFVSLLTGGLVWILLEILG
                     VEYAILWAILTFLLNYVPNIGSILAGIPIVIQALVLNGFSIGLGTLIGIVVINMVVGN
                     ALEPKLMGKKLGLSTLVVFLSLLFWGWLLGTVGMLLSVPLTMACKVALESNKATQKYA
                     VLFSDLEEVPKEQKYR"
     gene            complement(4747..5172)
                     /gene="ruvX"
                     /locus_tag="PXA33_001371"
     CDS             complement(4747..5172)
                     /gene="ruvX"
                     /locus_tag="PXA33_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HDL5120696.1"
                     /translation="MTETTTKTVIAFDFGTNSIGCAVGQSITGTAQGLTTFKAQDGIP
                     NWEEIGKLLQDWKPDMLVVGLPLNMDGTEQPLTQRARKFANRLNGRFNLPVELQDERL
                     TTVEAKAEIFSRGGYKALKKDKVDSISACLILESWFENH"
     gene            complement(5172..5732)
                     /locus_tag="PXA33_001372"
     CDS             complement(5172..5732)
                     /locus_tag="PXA33_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HDL5120697.1"
                     /translation="MLENLQGKFLIATPEMDDDYFERTVVYICEHNPQGAMGVVINTP
                     TDLSVLELITRMDFQMANQRDYKKDQMVLSGGPVNQDRGFIIHTKTANDFIHTYPITD
                     KITLTTSGDVLDSFGTLASPEKFIVCLGCSTWRGSQLEQEIAQNYWIVSDANEKTLFE
                     TGYLERWAEAYEMMGLSSIPSRAGRA"
     gene            complement(5732..6463)
                     /gene="rsmE"
                     /locus_tag="PXA33_001373"
     CDS             complement(5732..6463)
                     /gene="rsmE"
                     /locus_tag="PXA33_001373"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007524366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HDL5120698.1"
                     /translation="MRIPRIYQPQPLAGLQSCVLSEDAANHVGRVLRMKQGEQIILFD
                     GSNHVFHATLQAVEKKQIIAKIDSSELDDRESNLPIHLGQVISRGDRMEFTIQKSVEL
                     GVKTITPLWSERCGVKLNDERQDKKLQQWQKIAIAACEQCGRNEIPEIRPIMKLTDWC
                     KEQDDMLKLNLHPRAKYTIRELPNVPKAGVRLLIGSEGGLSAEEIAMTESEGFTEVLL
                     GKRVLRTETASLAAITALQVCFGDI"
     gene            complement(6523..6813)
                     /locus_tag="PXA33_001374"
     CDS             complement(6523..6813)
                     /locus_tag="PXA33_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL5120699.1"
                     /translation="MPKKLHNIYYTEEALIDLENIAISVSEFTGYASSGIRILEELEN
                     SINNLAIFPTMGVKGAIINTRELYHNGYRIVCEVKENEISIITIVHCSRLYP"
     gene            complement(6794..6967)
                     /locus_tag="PXA33_001375"
     CDS             complement(6794..6967)
                     /locus_tag="PXA33_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5120700.1"
                     /translation="MNNPYHNVSQEYLDYLAKKVKQGKEDIKNGRIYSPEEVRKRVIA
                     AAFRGAENAKKVA"
     gene            complement(7139..7726)
                     /locus_tag="PXA33_001376"
     CDS             complement(7139..7726)
                     /locus_tag="PXA33_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5120701.1"
                     /translation="MKRSKKTLVLATTFVAALLAGCQSQSNTLTFSTPTPTAVFNTNN
                     QTAAVNVMTQDLRTSREIASYTKNGEVIRLTAVPEVAAMFQQAVQQDLNAKGFQIVQG
                     AANANVVVNIRKFFATVEQGNLRYKADAQVSVKVAVQGSKGNFSKNFNATRGYEGAFG
                     ASNDEIKKVLDQAYSDVVKAIYNDNEIANAIHQFK"
     gene            7818..8129
                     /locus_tag="PXA33_001377"
     CDS             7818..8129
                     /locus_tag="PXA33_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HDL5120702.1"
                     /translation="MSVEQTIIQKIKTEFQPIFFNVENESYMHSSGRGAESHFKLTLV
                     SDKFEGQRTVARHRAVYACLAEELANGVHALALHLYTQGEWEATGGLVPKSLNCLGVG
                     Q"
     gene            8221..8310
                     /locus_tag="PXA33_001378"
     tRNA            8221..8310
                     /locus_tag="PXA33_001378"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8255..8257,aa:Ser,seq:gga)
     gene            complement(8486..8860)
                     /locus_tag="PXA33_001379"
     CDS             complement(8486..8860)
                     /locus_tag="PXA33_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein NlpE N-terminal
                     domain-containing protein"
                     /protein_id="HDL5120703.1"
                     /translation="MKKIALIGFTTAMLGACSLLPQKSVSGTYAGELPCADCEKIQAQ
                     LTLNADKTYQYDTVYFKDKKEYTYRDKGTYSWEPNKPNVIRLEKASGNLAFQVSEQHV
                     ELCDPNGNTVKNSNNYKLGKVR"
     gene            complement(8885..9187)
                     /locus_tag="PXA33_001380"
     CDS             complement(8885..9187)
                     /locus_tag="PXA33_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HDL5120704.1"
                     /translation="MKNSAPKKINDILQNSSLSRIVEKANFINDLNQKIQKRWPESYR
                     GLYRVVNLVDNQLIVSVKNATVRQGLILQQNMLLKQIQTDLPEITQLEFRVNPSVT"
     gene            9308..9628
                     /locus_tag="PXA33_001381"
     CDS             9308..9628
                     /locus_tag="PXA33_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2547 family protein"
                     /protein_id="HDL5120705.1"
                     /translation="MSLFLKHFHRTPFWSQLFLGMVAILALPEIQVAYANESEQETVL
                     NQSFLQYAQSANDIEQQSLFIAELIHKIVEIKPQAVHFCEFFANSYRLDRQNAYPIRA
                     GPQT"
     gene            9687..12413
                     /gene="secA"
                     /locus_tag="PXA33_001382"
     CDS             9687..12413
                     /gene="secA"
                     /locus_tag="PXA33_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HDL5120706.1"
                     /translation="MISKLLTSIFGSSNDRTLKRLRKRVAQINKLEPTFEQLSDEALK
                     DKTAEFKQRLADGVSLDSLLPEAFATVREASKRVLGMRPFDVQLIGGMVLTGRNIAEM
                     RTGEGKTLTATLSCYLNALTGKGVHVVTVNDYLARRDAETNRPLFEFLGLTVGVNIPG
                     LPSDAKRAAYQADITYSTNSELGFDYLRDNLAHDKNERFQRELHYALVDEVDSILIDE
                     ARTPLIISGPAEDATQIYQAVDQIIPHLIAQDKEDSEEYTGEGDFTLDLKNKQAHLTE
                     RGMVKVEDFLTQMGLMEEGESLYHPARISLLHHVSAALRAHKLFERNVDYIVKDGEIV
                     IIDEHTGRTMAGRRWSDGLHQAIEAKERVAIQGENQTVASITYQNYFRLYEKLAGMTG
                     TADTEAFEFQQIYGLNTVVIPTNRPVIRDDRTDLMFKSEAEKFAAIITEIRDCIERKQ
                     PVLVGTASVEKSELLSAELTKAGIAHNVLNAKFHAQEAEIVAEAGAPGAVTIATNMAG
                     RGTDIVLGGNWKMEVAKLENPTQEQIDAIKAAWQERYDIVMQAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDPGSSRFYLSLDDALMRIYLNEGKLNMMRKAFTEEGEAMESKLLT
                     KVIASAQAKVEAHNFDGRKHLLQYDDVANEQRKVIYEQRNHLLETDNIASMIESIRQD
                     VFESTINQYIPPQSIEEMWNVPGLEERLRREFGLDLPISHWLETEKDLHEETLRERIL
                     NIAIAEYNAKEEIVGAEVMRNFEKGVMLQNLDELWKEHLASMDYLRKGIHLRGYAQKD
                     PKQEYKKESFAMFTNMLDTLKLNVISILSRIQVRSKEEVEEAQRQQAEMDAASTPVTQ
                     ATDSAAGSREENSAESLSEEELANLNIGRNDPCPCGSGKKYKHCHGNKARYS"
     gene            12500..12898
                     /gene="mutT"
                     /locus_tag="PXA33_001383"
     CDS             12500..12898
                     /gene="mutT"
                     /locus_tag="PXA33_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HDL5120707.1"
                     /translation="MSKPIIQVSAGIIRNEFGQIYLTQRLEGQDFAQSLEFPGGKVDP
                     NETPEEALKRELEEEIGIQVLSAFPYENFCFEYPTKIIEFFFYLVEEWVNEPYGREGQ
                     EGFWIAQSDLDEGAFPPANAQLIKRLKEHA"
BASE COUNT         3808 a         2474 c         2686 g         3958 t
ORIGIN      
        1 gtgttatatg gcggttggct cttttgggta gtattagctt tttcctttat ctattttatt
       61 cttcgaatgg ttacttattt agtttatcgt caactgaatg aagagcagat tattaaagga
      121 gcaaaagccc attcgcattt tatggaaact ttatatggta ttacaacgtt aaaatcatta
      181 ggattggaaa ataaacgaca agaacagtgg atgtcgctca atgcggatgc ttttaacaca
      241 tcaattaagg taactaaatt tgatatgctg ttttccggta ttcatacctt tatttctacc
      301 atcgaacaga ttttaattct ttggattggg gcgacaatga tgattgaaaa tgtgatgaca
      361 ttaggaatgt ttgtcgcttt taatgcctat cgaggctctt tctcttcacg aatggggaat
      421 ttaattgata ttatttttag cctaaaaatt ctttcattac atagagagcg gattgcagac
      481 attgctttaa atgaagtcga agatgaaagt aaatatcaag aactcagatt tgaacataac
      541 caaacagata tagaagttaa agacctttgt ttcaaatacg atcctttttc taaaaatgtg
      601 cttgaaaatg tcaatttagt gatcagaagt ggggaaagtg ttgctattac cgcaccatca
      661 ggttatggca aaacaacgtt attaaaatta attgcaggtt tattaaaacc aacctcaggt
      721 tctgtgtatt ttaacggcat tgatatttat cagttaggat taagtcaata tcggagccaa
      781 attgcttgcg tattacagga agataaattc ttttctggtt ccattttaga gaatattgtt
      841 agttttgaaa gtaattatga tcgagaatgg gctatcgaat gtagcaaact tgcgaatatt
      901 cacaatgata ttatggcaat gccgatgaat tatgaaacct tattaggaga attaggaaat
      961 aatttgtctg gcggacaaag ataacgattg tttattgctc gagctttata taaaaagcct
     1021 aaaattcttt tgatggatga ggcgactagc catttagatg aagaaaatga gaagcttgtc
     1081 aatcaagcta tttcacgaat gaagataaca aggattatta ttgctcatag acaatcgact
     1141 attcagtcgg cggatagaat tattgccctg aatgacaggt aaattaattc ctatttttct
     1201 gttcttgccg ataaagttta ttgcacggaa tgctgtcgtt gttgccgtct agctctttcc
     1261 agccgcattg ttgaaagtag cgttttgcct gtgcgtaatt gccgatttgg ttgcagctta
     1321 atttaatttg gcaatcaatc gttggtggct ggtttgcaat attttccggt tttttgaccg
     1381 cttgtggtaa ggttgaggtg tgtgtttttc gccattggct cgggttaatc gggaatttgt
     1441 cctgccataa gccgatttta gcttgctgtg cttgatcttg tgcttcgata tattcccgct
     1501 ttgtggtgta agcccacgcc ataccgtttt ccactaaggc taagttgatg tttttatcgt
     1561 ttaagtagag ggtggcaagt aatcgttggt aacgatcata gcctgaaact atggcagtaa
     1621 tgttttgttg aaacacgaat tgagccagag cttgtttggc tttgttgccg taaggctgcg
     1681 cttgttcggg ggcatcaata tgttgtagcc ttatttttag tggtgtttta ttgaccaaac
     1741 aagtgagggt gtcgccatcg ctaatgccaa cgactttgca ttgtagttgg cgttcggcgg
     1801 catataccga tgagaaaaat aagctaaaaa tcagggcgta aagatatttc attattaaaa
     1861 aagagagggc taataagccc tcattctatc attttttatt cggctttacc tgttttaagg
     1921 cgagcgaaga aatcttttgt ttcactaata atcactttgc gtaacgcaat caatgcgatt
     1981 aaattcggga atgccattaa gccgttcacg atgtctgcca gcgtccaaat tgtatcgagt
     2041 tgtaaaaatg gagcagaggc gatgagtgca ataaacagca agcggtataa tttaatgtat
     2101 tgggtgcgac ctcgagtgag atagacaaaa cagcgttcac cgtagtagca ccagcctaaa
     2161 atggtggtaa aggcaaagaa gactaaacca actgtcacaa ttatcgcacc gaatgagctg
     2221 tttaaacctt gagtaaaagc ggttgtggtg agttctgcgc cttttacatc gcctgcccaa
     2281 gcccctgaaa tcacaattac taagccggtc attgtgcaga cgataatagt gtctaagaac
     2341 gtgccggtca ttgaaattag gccttgacgt accggctctt tggtttgggc ggcagcggcg
     2401 gcgatggggg cagagcctaa tcctgattca ttcgagaaaa taccacgtgc aacccctaat
     2461 tgaatcgctt gcatgacggt gaatccgact gctccgccta atgccgcttg tgggttgaaa
     2521 gctgcgtgta caatagtaat aattgcttgt gggacttttt ccatattcag tagaatgacg
     2581 gataaagcgg cgatgacata cccaatagcc ataaacggta caacaatggt tgcaatttta
     2641 gaaatccgct gcacgccacc taaaataata gcggtaacga taagggttaa aatgatcgca
     2701 ctgaagataa tcggcacatc aaaggtgctt tctaaggcgt gggtaatgcc attcacttgg
     2761 gggaacgtgc caataccaag caaagcgacc aatacgccaa aaaacgcaaa ggctttagca
     2821 agccaacgcc atttttttcc cattcccagt tcgatatagt acatcggacc accagccaca
     2881 aatccggctc tatctttagt tcgatattta attgccaata agccttcggc atatttcgta
     2941 gccataccga ataaggcaat cagccacatc caaaataatg ctccaggccc gcccgaatga
     3001 atagcggttg ccacgccgac aatattcccc gtgccgatag tagctgctag ggcagtggaa
     3061 agggctgcaa aagcggaaat atcgcccgat tgccctttgc ctttttccgg tttgaacata
     3121 tagcgaaagg cttgccctag ttggcttaat tgaatacctt tgagtgaaaa ggttaaatat
     3181 aaccctacgc ctgaaattaa aatcaggagt ggcgggcccc aaacaaaact gttgatttga
     3241 ttaagaatag tgtcgataga catttgatgt tcctcgtaaa aactgcttac aaggaaagaa
     3301 aaggaatgca agagacaagc ggtcaaaaaa cggaaatttt ttgcaaagaa ttagcaaaaa
     3361 aacaccgctt gtatcgtata acttttctcc cctgtccttt tgcctgagcg tttcacacat
     3421 tgctgtattt tcgccttcgg cggcggattt taatccgctc tctccaaggg ttcgtccagt
     3481 aacagtcccc gcagtgttca ctgcacctga aagagtttac ctcgtcggtg aaatgttaat
     3541 tcatttctct ccagctacct tcatccgaac aatctaattt attttataat gtaaaataaa
     3601 aaagatttgc caaattctag caactctttt gcttccgtca agtgaattta acgatatttt
     3661 tgttctttcg gtacttcttc taaatcgcta aataatacgg catatttttg ggtggcttta
     3721 ttggattcta atgccacttt acacgccatc gtaagtggaa cagaaagtaa cattcctacg
     3781 gtgccaagta accaccccca aaatagaagt gagaggaaca ctacaagggt agaaagcccg
     3841 agtttcttgc ccattaattt aggttcaagc gcattaccca caaccatatt aatcacgaca
     3901 atgccgatta aagtgcctaa tcctatagaa aagccattga gtaccaaggc ttgaatcaca
     3961 attggaattc ccgccaaaat agagccgata ttcgggacgt agttaagcag gaaggttaga
     4021 attgcccata aaatcgcata ttctacgcct agaatttcta gtaagatcca aactaaccct
     4081 ccggtgagta ggctcacaaa ggttttaact ccaaggtaac caatcacccc ttcaagtact
     4141 tggttgatat actctttttc gttgcttaaa tcatcatctg ggctaagtgc gagggctaat
     4201 ttatatttgg caaccggcat ttccaatagc ataaaaatga cgactaagaa aatcacaaaa
     4261 atattcgata atgcaccgga tagccccatt agcagtttgc tgaccaaatt cattattgag
     4321 cttgggtcaa atttgctcat aatctcttct tgtgagaggg taagtggaat atgccatcta
     4381 tgggcgagtt ctagaatcac ctggattcta gatgaaagct gaattttgta ttgcggaata
     4441 gcattagtaa attcttgcaa ggtgttatta ataatgcctg ctaagaaact gaatcctaag
     4501 ataaaaacaa taagtaaaat ggtaatggct aaccataatg gcactttacg cttagtcata
     4561 aatttgatca ttggtgaaca aataatggca ataaatagcg aaagtaaaaa tggcacaaca
     4621 atttcattgg ataatttaat ccctgccaga ataatgataa tggaggctat cgccacgaga
     4681 gttttggtgg aatgtgtcat aatttttcct tttacaagcg gttaaaaatc agcaattttt
     4741 tacaaattag tgattctcaa accaactttc taaaattaaa caagcggaaa tggaatccac
     4801 tttatccttt tttaatgctt tgtaaccacc acgagaaaaa atttccgctt ttgcttcaac
     4861 agtagtgagg cgttcatctt gtagctcaac cggcagatta aagcgtccgt ttaagcgatt
     4921 ggcaaatttt ctggcacgct gagttaaagg ctgctctgtg ccgtccatat tcaagggtag
     4981 cccaacaacc agcatatctg gtttccaatc ttgcaagagt tttccgatct cttcccagtt
     5041 tggaataccg tcttgggctt taaatgtagt taaaccttga gctgtgccgg taatactttg
     5101 tccaactgca cagccgatgc tatttgtgcc aaaatcaaag gcaatcacgg tttttgttgt
     5161 ggtttctgtc attaagctct cccagctctt gacggtatgc ttgaaagccc catcatttca
     5221 taggcttctg cccaacgctc taaataaccg gtttcaaaca gcgttttttc attcgcatca
     5281 gacacaatcc aataattttg agcaatttct tgctcaagct ggctgccacg ccaagtggaa
     5341 caacctaaac acacaataaa tttttcaggt gaagccagtg tgccaaagga gtctaacaca
     5401 tcaccggagg tagtgagcgt aattttatcg gtgatcggat aagtgtgaat aaaatcattg
     5461 gcggttttgg tgtgaataat aaagccacga tcttgattta ccggcccacc gcttaacacc
     5521 atttggtctt ttttgtaatc acgctggttc gccatttgga aatccattcg ggtaatcagc
     5581 tctagaaccg acagatcggt aggagtgtta attactactc ccattgcacc ttgcggatta
     5641 tgttcgcaaa tatagaccac cgtacgctcg aaatagtcat cgtccatttc cggcgtggca
     5701 attaaaaatt taccttgtaa attctctaac attaaatatc tccaaaacaa acttgtaacg
     5761 cagtaattgc cgcaagtgag gcggtttctg tgcgtaacac tcgtttaccc agtaatactt
     5821 cagtaaaacc ttcgctctcg gtcatggcga tttcttcggc agataatccg ccctcagagc
     5881 caatcagtaa acgaacacca gctttcggca cattcggcag ttcacgaatg gtatatttgg
     5941 ctcttgggtg caaattcagt ttcaacatat cgtcttgttc tttgcaccag tcggtgagtt
     6001 tcataatcgg gcgaatttca gggatttcat ttcgtccgca ttgttcacag gcggcaatcg
     6061 caattttctg ccattgctgt aattttttat cttgccgttc atcattgagt ttcacgccac
     6121 aacgttccga ccaaagtggg gtaatagttt ttacccccag ctccaccgat ttttgaatgg
     6181 taaattccat tctatcgcct cgggaaatca cttgccctaa atggatcggc aggttggatt
     6241 ctcggtcatc taattcactt gaatcaattt ttgcaataat ttgctttttt tcgaccgctt
     6301 gtaacgtagc gtggaaaacg tggtttgaac catcaaataa aatgatttgc tctccttgtt
     6361 tcattcgtag cactcgccca acgtggttag cggcatcttc acttaacacg caactttgaa
     6421 gaccagctaa cggctgtggt tggtaaattc ttgggattct cataattatc tagttataaa
     6481 ttaaaggcag acgattgtct gccataggta agttaaaacg ggttaaggat aaagtcttga
     6541 acaatgcaca atagtaataa tcgaaatttc attctcttta acttcacaca ctattctata
     6601 gccgttatga tatagttctc tcgtattaat aattgcacct tttacaccca ttgtaggaaa
     6661 aatggctaaa ttattaatac tattttccaa ttcttctagt atcctaattc cactacttgc
     6721 ataaccggta aattcactaa ctgagatggc aatattctct aaatctatta aggcctcttc
     6781 agtataataa atattatgca acttttttgg cattttcagc acctctaaaa gcggcagcaa
     6841 taactctttt tctgacctct tcgggtgaat aaatccgacc gtttttaata tcttcctttc
     6901 cttgctttac ttttttagca agataatcaa gatattcttg gctaacatta tgataaggat
     6961 tattcatatt tttcctccct gcgatgagtc tctattgtgg gggctactga tcgtttaatc
     7021 aagttaattt atcttatttt tcgagccaca tcacaaaacg agaaaacaac aagcggtcat
     7081 ttttccacgt tttttgcaaa aaatcgaaga aagatgaccg cttgccagaa aaaattaatt
     7141 atttgaactg gtgaattgca ttggcgattt cattatcgtt gtaaatagct ttcaccacat
     7201 cagaataggc ttggtctaac actttcttga tttcatcatt gcttgcacca aatgcacctt
     7261 cgtaaccacg ggtagcgttg aagtttttgc taaagtttcc tttagaacct tgcactgcca
     7321 cttttacact cacttgtgca tcggctttgt agcgtaagtt tccttgttct actgtggcaa
     7381 agaatttacg aatattcact accacattgg cattcgctgc accttgtaca atttggaaac
     7441 cttttgcatt caggtcttgt tggacggctt gttggaacat tgcagcaact tcaggcactg
     7501 cggttaaacg aatgacctct ccgtttttag tgtagcttgc gatttcacgc gaggtgcgta
     7561 aatcttgtgt cattacattc accgcagccg tttgattgtt tgtgttaaat acagcagttg
     7621 gcgttggggt agagaatgtt aatgtgttgg attggctttg gcagccggct agtaatgcgg
     7681 caacaaaagt agtggctaac actaacgttt ttttagagcg tttcatagta aatccttcta
     7741 tattgaataa atttcgagaa aatttctgct tagtatagta ttatttcgtt ttgattttca
     7801 atgaacgaga gcaaaaaatg tcggtagaac agacaattat tcaaaaaatt aagacagaat
     7861 ttcagccgat tttctttaat gtcgaaaacg aaagctatat gcacagctca ggtagaggag
     7921 cagaatcgca ttttaaatta actttggtgt cagataaatt tgaagggcag agaacagttg
     7981 cacgtcatcg tgctgtctat gcttgcttag ctgaggaatt agctaatggc gttcacgcac
     8041 ttgctttgca cctatatacc caaggtgaat gggaagcgac tggcggactt gtacctaaat
     8101 cgctaaattg cttaggtgtt gggcaataag tggataaaaa tgatttattt tattttgtga
     8161 agaaaatact tgaaagagcc gtgtaaaatt gctatattta cgccatcttt ttgttgattc
     8221 ggtgagatgt ccgagtggtt gaaggagcac gcctggaaag cgtgtatatg ggaaactgta
     8281 tcgggggttc gaatccccct ctcaccgcca ttcatactga aagattcctt actgttttca
     8341 tagatatttc cttggttaag ttaaagtaga taatactttt tcatttttag gttatccccg
     8401 agcaaacatt gcttgggggt ttttttatgc gatcggcgat agattacaaa gaaaaaggat
     8461 tcctaagaat cctttttatc tgcttttaac gtactttacc tagtttatag ttattgctgt
     8521 tttttaccgt gttgccattt gggtcgcaaa gttcaacgtg ttgttcagac acttggaagg
     8581 caagattgcc tgatgctttt tccagacgaa tcacatttgg cttattgggt tcccaactgt
     8641 atgtgccttt gtctcggtaa gtgtattctt ttttatcttt aaaatatacc gtatcatatt
     8701 ggtaggtttt atctgcattt aaggttagct gggcttggat tttctcacag tcagcacacg
     8761 gtaattcacc tgcataagta ccgctaacgc ttttttgtgg tagcaatgag caagccccta
     8821 gcattgcggt ggtaaaacca atgagtgcga tttttttcat actattttcc tcttcaaaat
     8881 taaattatgt aacactcgga tttactctaa attcaagttg ggttatttca ggtaaatcgg
     8941 tttgtatctg ttttagtaac atattttgtt gcaagattaa accttgccgt acggttgcat
     9001 tcttaacgct cacaataagc tgattatcaa ctaaattcac tactcggtaa agcccacgat
     9061 aagattctgg ccatcttttc tgaatttttt ggtttaaatc gttaatgaag tttgcttttt
     9121 cgacaatacg gcttaagctt gagttctgta aaatgtcatt aatcttttta ggtgctgaat
     9181 ttttcatctg tttctttaca tttcgtctta ttttaggctt gattttacga tacttgaccc
     9241 aataatttac tcattcggct ttactttagc gtataattag cccaattttt tcatttatct
     9301 taatttaatg agcttatttt taaaacactt tcatcgaacg cctttttggt cgcaactctt
     9361 tttgggaatg gttgcgatac ttgctttacc tgaaattcag gttgcgtacg ccaatgaaag
     9421 tgaacaggaa accgttctta atcaatcttt tcttcaatat gctcaatccg ctaatgatat
     9481 tgagcaacaa tcgcttttta ttgcagagtt aatccataaa attgtggaaa ttaaaccaca
     9541 agcggtgcat ttttgcgaat tttttgcaaa ctcctaccgc ttggatagac aaaatgctta
     9601 ccccattcga gccgggccac aaacctaaat ataatttaat tttaccatta attgattaac
     9661 ccatttttat atttaggaat aacacaatga tttcaaaatt actgacttct atttttggct
     9721 caagcaacga tcgcacttta aaacgtttaa gaaagcgagt ggcacaaatt aataagcttg
     9781 aaccaacctt cgagcaatta tctgatgagg cgttaaagga taaaacagcg gagtttaagc
     9841 aacgccttgc agatggtgta tcgctagata gcctattacc tgaagctttt gcgacagtga
     9901 gagaagcaag caaacgagta ttaggtatgc gaccatttga tgtacagctg atcggcggta
     9961 tggtgctaac ggggcgtaat attgcagaaa tgcgtactgg ggaaggtaaa accctaactg
    10021 caactttatc ttgttatttg aatgctttga ccggtaaagg tgttcatgtt gttacggtga
    10081 atgattacct cgcccgccgt gatgcggaaa ccaaccgtcc gttatttgag tttttaggct
    10141 taaccgttgg agtgaatatt ccggggttac cttccgatgc aaaacgtgct gcataccaag
    10201 cagatattac ctattcgact aacagcgaat taggttttga ttacttgcgt gataacttag
    10261 ctcacgataa aaacgaacgt ttccaacgtg agttacacta cgcattagtt gatgaagtag
    10321 actctatttt aattgatgaa gctcgtacgc cgttaattat ttcagggcct gcggaagatg
    10381 cgacccaaat ttatcaagcg gtagatcaaa tcattccgca tttgattgct caagataaag
    10441 aagacagtga agagtacacc ggtgagggcg attttacctt agatctgaaa aacaaacagg
    10501 ctcatttaac cgagcgtggt atggtgaaag tagaggattt cttaactcaa atggggttaa
    10561 tggaagaggg cgaaagttta tatcaccctg ctcgtatttc gctgttacac catgtgagtg
    10621 cagcattgcg tgcccataaa ttatttgaac gcaacgtaga ttacattgta aaagacggtg
    10681 aaattgtgat tattgatgag cacaccggtc gtacaatggc tggacgtcgt tggtctgacg
    10741 gcttacacca agcgattgaa gctaaagagc gtgtggctat tcagggcgaa aaccaaactg
    10801 ttgcctcaat tacttaccaa aactatttcc gtttgtacga aaaattagcc ggtatgactg
    10861 gtacggcaga caccgaagcc tttgaattcc agcaaattta tggcttaaat acggtggtga
    10921 ttccaaccaa cagaccggtg atccgtgatg accgtacaga cttaatgttt aaaagcgaag
    10981 cggagaaatt tgcggcgatt attactgaaa ttagagactg tatcgagcgt aaacagcctg
    11041 ttttggttgg cacagcatcg gtggaaaaat cagagttatt atcggcagaa ttaaccaaag
    11101 ccggtattgc tcataacgta ttaaatgcga aattccacgc tcaagaggct gaaattgtag
    11161 cggaagcggg agctccgggg gcggttacta tcgcaaccaa tatggctggt cgtggtacgg
    11221 atattgtgct tggcggtaac tggaaaatgg aagtagccaa gcttgagaac ccgacacaag
    11281 aacaaattga tgcgattaaa gctgcgtggc aagagcgtta tgacatcgta atgcaagccg
    11341 gtggtttaca tattattggt acagaacgcc acgaatcccg ccgtatcgac aaccagttgc
    11401 gaggtcgttc tggtcgtcaa ggtgacccgg ggtcatcacg tttctatctc tctttagatg
    11461 atgccctaat gagaatttac ctcaatgaag gtaaattaaa tatgatgcgt aaagccttta
    11521 ccgaagaagg tgaagcgatg gagtctaaac tgttaaccaa agtgattgcc tctgcacagg
    11581 cgaaagtaga agcgcataac tttgatgggc gtaagcattt attacaatat gatgatgttg
    11641 ccaatgaaca gcgtaaagta atttacgaac aacgcaatca tctattagaa acagataata
    11701 ttgcctcaat gatcgaaagc attcgccaag atgtatttga aagcacgatt aaccaatata
    11761 ttccgcctca atctatcgag gaaatgtgga atgtgccggg cttagaagag cgtttacgcc
    11821 gtgaatttgg gttggattta ccgatttctc actggcttga aactgaaaaa gacttgcacg
    11881 aagagacttt gcgtgaacgt atcttaaata ttgcgattgc agagtacaat gcgaaggaag
    11941 aaatagtagg cgcagaggta atgcgtaatt ttgaaaaagg tgtaatgcta caaaacttag
    12001 atgagctttg gaaagagcat ctagcctcaa tggattattt acgcaaaggt attcacttgc
    12061 gtggttatgc acaaaaagat ccgaagcaag agtataaaaa ggaatctttt gcgatgttta
    12121 ccaatatgct tgatacctta aaattgaatg taattagcat tttaagccgt attcaagtgc
    12181 gtagcaaaga agaggtggaa gaagcgcaac gccagcaagc tgaaatggac gctgcaagta
    12241 cgccagttac acaagctacc gattctgctg ctggtagtag agaagaaaat agcgcagaga
    12301 gcttaagtga agaagagttg gcaaacttaa atattggtcg aaatgaccct tgcccttgtg
    12361 gttcgggcaa aaaatataag cattgccatg gtaacaaagc gagatattcg taattaacaa
    12421 gcggtcatat tcagtcagga atttgcaaat tttctgctta atatgaccgc ttgaatttat
    12481 agaaagagtg aaggaaacta tgtcaaaacc gattattcaa gtctcagcag ggattattcg
    12541 taacgaattt gggcaaattt acttaaccca acgtttagaa gggcaagatt ttgctcagtc
    12601 tcttgaattt cccgggggga aagtcgatcc caatgaaacc cctgaagaag cactaaaacg
    12661 agaattagaa gaagaaattg gtattcaagt gttaagcgct tttccgtatg aaaatttttg
    12721 ctttgaatat ccaacaaaaa tcattgaatt tttcttttat ttggtggaag agtgggtaaa
    12781 tgaaccttac ggaagagaag ggcaagaagg tttttggatc gcacaatcgg atttagatga
    12841 gggagctttc ccaccggcaa atgcccaatt aattaaacgc ttaaaagaac acgcataatt
    12901 tgtattgaga actgtatcta aagtaa
//
LOCUS       DANLRK010000070        12823 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.070, whole genome shotgun sequence.
ACCESSION   DANLRK010000070 DANLRK010000000
VERSION     DANLRK010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 12823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12823
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.070"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            11..703
                     /gene="uppS"
                     /locus_tag="PXA33_001384"
     CDS             11..703
                     /gene="uppS"
                     /locus_tag="PXA33_001384"
                     /EC_number="2.5.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-trans,poly-cis-decaprenylcistransferase"
                     /protein_id="HDL5120708.1"
                     /translation="MPKHVAIIMDGNGRWAKQKGKLRIFGHQNGVKAVRSAVSFAAKH
                     HIQVLTLYAFSSENWSRPETEVSALMSLFMKALDSEVKQLHKNNIRLKIIGDKSAFSE
                     NLQKRIAESEKLTENNTGLILNIAANYGGYWDIVQAAQQLAVHVKSEQLAVEQITPAL
                     FQQHLVTENQPQVDLLIRTSGEQRISNFLLWQIAYAELFFSPVLWPDFNDEAFSDAIY
                     AYQQRERRFGGC"
     gene            727..1596
                     /locus_tag="PXA33_001385"
     CDS             727..1596
                     /locus_tag="PXA33_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005622689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HDL5120709.1"
                     /translation="MLKERVLSAIIMIIAVLAAIFWLSPLPLTLVLSAIVVAAMWEWA
                     QFAGFKHPVPRAIVAMVTICLLLFAIVANTDYIRATRFITDETTPILFIGCIWWLIAL
                     VLVATYPNSAKLWEKSVAAKFLFGLATLVPFLIGTLALRFHNYAFDPYQGTYLFLYVC
                     LLVWGADSGAYFAGRAFGKRKLAPKVSPGKSWEGVIGGVLTSSLIAFTFLQLTPANVF
                     GKALATTPFILVSVGMVAISVLGDLAESMFKRQAGIKDSSQLIPGHGGILDRIDSLTA
                     AVPFFATFFFFVL"
     gene            1600..2910
                     /gene="rseP"
                     /locus_tag="PXA33_001386"
     CDS             1600..2910
                     /gene="rseP"
                     /locus_tag="PXA33_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HDL5120710.1"
                     /translation="MTSIIAFFILICVLVFVHEYGHFWAARKCGVKVIRFSIGFGKVL
                     FKKKDKQGTEFVFSLIPLGGYVQMWNDETDINAPAQQALNTKSVLQRAFIIIAGPAAN
                     FIFAILAYWVVFIAGIPTVKPVIGEILPNTIASQARIPTELEITKIGHQSIQDWESAS
                     LALVGFVGNKNVPLEGNINGNSTQKYELDLSNWSIDSAKENPLTALGIRPKSAKVTAE
                     IKQVVENSPASQVGIQAGDRILQINQQPFNWFNLVELVQAGKPIELKIEQRGQIKDLV
                     VQPEKKDERYIIGVIPSYEPLADKYRTELKYDILTAFYKSIEKVWSLTQTILQFIGNL
                     ISGDLSIKNLGGPISMAKGAGATAEIGLVYYLSFMALISVNLGVMNLFPLLPLDGGQL
                     VLLAVEAIRGKALSEKIQLKFQQIGFAFVLSLMLFAFANDIIHF"
     gene            3025..5406
                     /gene="bamA"
                     /locus_tag="PXA33_001387"
     CDS             3025..5406
                     /gene="bamA"
                     /locus_tag="PXA33_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HDL5120711.1"
                     /translation="MKKLLLSSLLIANGVVAAPFVVKDIRVEGVQPTTGESIISSIPV
                     RVGQTATDTDVSNVVRHLFSQQRFADVRATREGNTLVIKVAEKPIIGKVEIEGNQAIP
                     KDALEQNLKANLINQGEIFDAAKLEAFKEGLIEHYHSTGRYEAKIDTSVSNNTEGGVN
                     IKLAINEGEVAKAKTIKFEGNNAFSDKKLLENLDIQPDVSWWNIFESSKFEQTAYQKD
                     LETLRDFYLNRGYAKFAIQDTDVQFNDNKTEVALTYKLDEGSQYHVSEMRIIGNTANM
                     DSQLNALLKEFKPGQLFRKSDLTQIEEEIKSILGDAGYGSAKVDIYPQFNEENKTVKI
                     SFVVDAGRRIYVRKIRFEGNGVTADSTLRREMRQQEGAWLSTNKASLGKARLERTGFY
                     ESVDMSMPNVEGTADQVDVVYKIKERNTGSINFGIGYGTENGISYQAGIKQDNFLGMG
                     STISLNGTRNNYGTSVNLGYTEPYFTKDGVSLGGNIFYEDYDNSDNDSMASYKRQTYG
                     INGTLGFPVNENNSYYLGLGYTHDKIKNAAREYTREKYVKSMNFEFDPAADYYKKIKA
                     DDFDFSLGWNYNSLNRGYFPTEGTTANIGGKITIPGSDNKYYRVSADFRNYYPLNREH
                     KWVISTKASIAYTNGFGGKEVPFYQLYTAGGIGTLRGFSYGAVGPQAIYYNTKSGKFD
                     SMNNDIIGGNALAAASLELITPTPFVSEKYQHNVRTSLFVDAASAWNTKWKKDEYTNL
                     PDYGDFKRFRASAGIAFQWMSPIGPLSFSYAKPIRKYDNDDIEQFQFNIGSSF"
     gene            5486..6277
                     /locus_tag="PXA33_001388"
     CDS             5486..6277
                     /locus_tag="PXA33_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HDL5120712.1"
                     /translation="MKKLFKMTGLAVALASTTGIANAADTIGFVDPSYVLQNHPVLLD
                     ASAKFDKFMKESQEKFAADEKKLADENKTLTAERNKINEDAQKLQNEQKNVEASIKKK
                     VAALEKDAPRLRSKEIQARQNAIQKEANAFQAKVTAIQKREADFAKKAEAFQKRADEF
                     QKRIEQANKENGGINPQEAQKQAVDEVNATIKEIAKSKGYTLVLQPQVALYAEDEGKD
                     ITEAVLDAIVKKHPEIKIEKPATEAQPATEKETKAEETKPEEAKK"
     gene            6277..7302
                     /gene="lpxD"
                     /locus_tag="PXA33_001389"
     CDS             6277..7302
                     /gene="lpxD"
                     /locus_tag="PXA33_001389"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HDL5120713.1"
                     /translation="MANFRLIDLAEHIGGTLRGNADLAIQSIAALDKADSSQITFISN
                     AKYRENLTACNAGAIIVSPADVEFCRADQNLIIVANPYVAYAQLAQYMDSTPKAASEI
                     HPSAVISPEAKLGNNVSVGANAVIESGVELGDDAVIGAGCFVGKNSKIGARTQLWANV
                     SVYHNVQIGADCLIQSSTVIGSDGFGYANDKGQWIKIPQTGGVIIGNRVEIGACTCID
                     RGALDPTVIEDNVIIDNLCQIAHNVHIGFGTAVAGGVIMAGSLKVGRFCQIGGASVIN
                     GHMEICDGAIITGMGMVMRPITEKGIYSSGIPLQTNKEWRKTAALVMNIDEMNKRLKS
                     LEKRFND"
     gene            7331..7789
                     /gene="fabZ"
                     /locus_tag="PXA33_001390"
     CDS             7331..7789
                     /gene="fabZ"
                     /locus_tag="PXA33_001390"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDL5120714.1"
                     /translation="MTTENREPNIIEVTEIMDMLPHRYPFLLVDRVTDYEEGKWLKAI
                     KNVTFNEPCFTGHFPGAPIFPGVLILEAMAQATGVLAVATYGKLSEDELYYFAAIDNA
                     RFKRPVVPGDQLQLEVEFLKEVRGITKFTGKAYVDGKLVCEADLMCARRK"
     gene            7823..8617
                     /gene="lpxA"
                     /locus_tag="PXA33_001391"
     CDS             7823..8617
                     /gene="lpxA"
                     /locus_tag="PXA33_001391"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HDL5120715.1"
                     /translation="MRLIDSTAKISPLAIIEEGAEIGAHVEIGPFSVIGKDVKIGART
                     KIHSNVVINGMTEIGEDNHIFQFASIGEINQDLKYQGEPTKVVIGNRNRIRESVTIHR
                     GTVQGGGVTKIGDDNLFMINTHIAHDCSIGNRCIIANNGTLAGHVTLDDFVIVGGMSA
                     IHQFVVIGSHVMLGGGSMVSQDVPPYVMAQGNHARPFGINFEGLKRRGFDKPTMHAIR
                     KVYKLIYSSGKTLEECLVEIEQIAATEPAIAIFKQFFKRSTRGIIR"
     gene            complement(8655..10655)
                     /gene="mnmC"
                     /locus_tag="PXA33_001392"
     CDS             complement(8655..10655)
                     /gene="mnmC"
                     /locus_tag="PXA33_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HDL5120716.1"
                     /translation="MGKLTFATLTFNDADTPVSEQFDDIYFSTQDGLAESYYVFQEGN
                     QLWEKWQSHPKEAFVIAETGFGTGLNFLAVADKFQQFRAAFPQSKLKRLYFISFEKFP
                     LTASQLADIHQRYPQFATLSENLTACWQPRQIGCQRYHFEQVYLDLWFGEIAENLPQL
                     GDLYSGTIDAWFLDGFSPDKNPEMWNEQLYLQMFRLSANGGSFATFTASSMVRRGLEA
                     AGFAVKKRKGFGKKREMLWGEKPLDSQVSEPNYPYFYAKNETADDIAIIGGGVASFFV
                     ALSLLEKGKQVTLYCKDETLAINASGNLQGAIYPQLSDDDERNVQFYIHSFDYALQRL
                     QQLKSNIEFEHNLSGVVLYAYDEKTAKKLEKMVEQTADDTLLKSCSQAELSEKLGLSV
                     PNSGAFIPQAGWLSPVQLVRNGFTWLEQKGLKIVLNHKVEHLTFSNGMWQWQTRGETV
                     RHQAVVLANGHTLNEFSQANGIPLYPVRGQVSQIPTTSELQKLKCVLCYDGYLTPMSA
                     YGTHCIGASHIRDNAETEFSLSEHWENVAKLQQNVTVCEWTQGIDLSENKAKVGIRAA
                     FRDRVPMVGAVHHFEQQKSQYANLYNQLRRKQAVENAEIFTNLYMVNGLSSRGLTTAP
                     LLGELLASLIVNEPVPISEDIWHNLSPNRAWIRKLLKGSTVA"
     gene            complement(10880..12316)
                     /gene="trpCF"
                     /locus_tag="PXA33_001393"
     CDS             complement(10880..12316)
                     /gene="trpCF"
                     /locus_tag="PXA33_001393"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HDL5120717.1"
                     /translation="MQNIVQDNAQPNQPTILQKIVQDKAIWVAQKEQAFPLSEFQHKI
                     VKSDHDFYAALAKGTHQEPAYILECKKASPSKGLIRAEFDLDSIAQVYKNYAAAISVL
                     TDEQYFQGDFRYIDQVKRQTTQPILCKDFMISAYQVYLARFYNADAILLMLSVVNDET
                     YRELATLAHELGMGVLTETSTEAEFERALALGAKVIGVNNRDLHTLTIDMNRIVRLVE
                     KYQAQISADVCLISESGINDHQQVKNIKPYVHAFLIGSSLMGSADLNNAVRAVIFGEN
                     KVCGLTRVQDVKAVYEQGALYGGLIFAEGSPRQLSLRQAQELVVNAPLRYVGVFQNQA
                     VEFVEKMAKQLELFAVQLHGSEDEQYIAELAEKLGSKTQIWKALSVDVEVENLEFNDN
                     PQIARYVLDSKVGNQQGGTGKTFNWALIPETLKQKALLAGGIGTENIEQALAQGCVGL
                     DLNSGVESAKGVKDLQKLTACFGKIIQA"
     gene            12404..12715
                     /locus_tag="PXA33_001394"
     CDS             12404..12715
                     /locus_tag="PXA33_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HDL5120718.1"
                     /translation="MKSQVIQHLSYLEIALRVNQLWEATPPSPEALANEQPFCVETLT
                     PTQWLQWIFIPRMRAILDANADLPRNFAITPYLEEALKNEGYLQAICEPLLGLEKLLK
                     D"
BASE COUNT         3981 a         2591 c         2523 g         3728 t
ORIGIN      
        1 tcctcaaaat atgcctaagc acgttgccat tattatggat ggcaatggca gatgggctaa
       61 gcagaaagga aaattacgca tttttggtca tcagaatggt gtaaaggcag ttcgttctgc
      121 agttagtttt gcagcaaagc atcatatcca agttttaacc ctctacgcat ttagcagtga
      181 gaattggagc agaccagaaa ctgaagtttc agctttaatg tctctgttta tgaaagcttt
      241 agattctgaa gttaaacaat tacataaaaa taatattcgc ttaaagatta taggcgataa
      301 atccgccttt agcgaaaatt tacaaaaacg cattgccgaa tcggaaaaat taaccgaaaa
      361 caatactggg ctaatcttaa acattgctgc caactatggt ggttactggg atattgttca
      421 agctgctcaa caactagcag tgcacgttaa atctgaacaa cttgctgtgg agcagattac
      481 accagcattg tttcaacaac atttagtgac agaaaaccag ccgcaagtgg atttattaat
      541 tcgaaccagt ggtgagcagc ggatcagcaa ttttctatta tggcaaattg cttatgccga
      601 gctgtttttt agcccggttt tatggcctga ttttaatgat gaggctttta gtgatgcaat
      661 ttatgcttat cagcaacgtg aacgccgatt cggtggttgt taatataaaa agaataagga
      721 aatacaatgc ttaaagaacg tgtactttca gcgattatta tgattattgc cgtgctggct
      781 gccatatttt ggctctcacc acttccttta actttagtgt tatcggcaat tgttgtggct
      841 gcaatgtggg aatgggctca atttgccggc tttaaacacc ctgtgccacg tgcaattgtg
      901 gcaatggtga caatctgctt attacttttt gcgatcgttg cgaatactga ttatatccga
      961 gccacccgtt ttattactga tgagactacc cctatcctct ttattggctg tatttggtgg
     1021 ttaattgccc ttgtcctagt ggcaacttac cccaactcgg caaaactgtg ggaaaaatct
     1081 gttgcagcta aattcctatt tggacttgct accttagtgc cgtttttaat tggcacgctc
     1141 gcattgcgtt tccataatta tgcatttgat ccataccaag gaacttattt attcctttac
     1201 gtctgtttat tagtttgggg agctgattca ggtgcttatt ttgctggcag agcctttggc
     1261 aaaagaaaac ttgccccaaa agtatctccg ggtaaatcgt gggaaggcgt aattggtggc
     1321 gtactcacct ctagtttgat tgcctttacc tttttacaac ttacaccggc aaatgtattc
     1381 ggcaaagcat tagccactac cccatttatt ttagtttcgg tcggtatggt tgctatttca
     1441 gtcttaggcg atttggcaga aagtatgttt aaacgccaag caggcattaa agacagtagc
     1501 cagttaattc ctgggcatgg aggtatttta gatcgtattg atagtttaac cgctgccgtt
     1561 ccgttttttg ctacattctt cttttttgta ctatagaata tgacgtccat cattgcattt
     1621 tttattttaa tttgcgtact ggtttttgtg catgaatacg ggcacttttg ggctgctcgc
     1681 aaatgcgggg taaaggtgat ccgtttttcg atcggatttg gtaaagtctt atttaagaaa
     1741 aaagataaac aaggaaccga atttgttttt tcacttattc ctctgggcgg ctatgtacaa
     1801 atgtggaatg atgaaacgga tatcaacgct ccagctcaac aagctcttaa cacaaaatct
     1861 gtattgcaac gtgcatttat tattatagcc ggtcctgctg caaattttat ttttgcgatt
     1921 ttggcatact gggtcgtttt tattgccggt attccaactg ttaaacctgt tattggggaa
     1981 attttaccaa acactatcgc ctcccaagca agaattccta ccgaattgga aattacaaaa
     2041 atcggtcatc aatctatcca agattgggaa agtgcatctt tagcactagt tggttttgtg
     2101 ggcaataaaa atgtaccgtt agagggaaat atcaacggca actcaacaca aaaatatgaa
     2161 ctcgatttat ctaactggag tattgactca gccaaagaaa acccattaac cgcattaggt
     2221 attcgcccca aaagtgcaaa agtcaccgct gaaattaaac aagttgtcga aaattctcct
     2281 gcaagccaag tgggtattca agcaggtgat cgtattcttc aaattaacca gcagccattt
     2341 aattggttca atttagttga actggtacaa gcaggtaaac ctattgaact taaaattgaa
     2401 caacgaggtc agattaaaga ccttgtcgtc caaccagaaa aaaaagacga acgctatatt
     2461 atcggggtaa taccaagtta tgagcctctt gcagataaat atcgtaccga attaaaatat
     2521 gatattctta ccgcatttta taaaagtatc gaaaaagtgt ggtctcttac ccaaaccata
     2581 ctccaattta tcggtaattt aataagcggt gatttgtcca ttaaaaattt aggtgggcca
     2641 atttcaatgg caaaaggagc cggtgcaaca gctgaaatcg gcttagttta ttaccttagt
     2701 tttatggctc taatcagtgt caatttgggg gtaatgaact tatttccact tttaccctta
     2761 gatggtgggc aacttgtttt attggctgtt gaagcaattc gtggaaaagc attatcagaa
     2821 aaaatacaac tcaaattcca acaaattgga tttgcattcg tgctgagttt aatgttattc
     2881 gcctttgcca acgatataat tcacttttaa caagcggtta aaaaaggtga cttttttgca
     2941 aaaagcaccc tgacatcaaa atccataata tggatttaaa ctatattctt aataattttg
     3001 caaaacgatc ataggataat ttcaatgaaa aaattattac tttcctctct tttaattgca
     3061 aacggtgtag ttgctgcacc tttcgtagta aaagacattc gtgttgaagg tgtgcaacct
     3121 acaacaggcg aatctatcat ttcatctatt ccggttagag taggtcaaac agcaacggat
     3181 actgacgtat ctaatgtcgt ccgccatcta ttcagccaac aacgttttgc agatgttcgt
     3241 gcaacacgag aaggcaatac gcttgtcatc aaagtcgctg aaaaaccgat tattggcaag
     3301 gtagagatcg aaggtaacca agcgattcct aaagatgcct tagagcaaaa tttaaaggca
     3361 aacttaatta accaaggtga aatttttgat gctgccaagc ttgaagcttt taaagaagga
     3421 ctaatcgaac attatcactc taccggccgt tatgaagcca aaattgacac atctgtttcg
     3481 aataacaccg aaggtggcgt taatattaag ttagcgatta atgaaggtga agttgctaaa
     3541 gccaaaacaa ttaagtttga aggtaataat gccttctctg ataaaaaatt attagaaaac
     3601 ttagatattc aacctgatgt ttcttggtgg aatatttttg aaagtagcaa attcgaacaa
     3661 accgcctatc aaaaagactt agaaacatta agagattttt atttaaatcg tggctatgca
     3721 aaatttgcta tccaagatac cgatgttcaa ttcaatgata ataaaacgga agttgcattg
     3781 acctataaat tagatgaagg gtcacagtac catgtcagtg aaatgcgcat tatcggtaat
     3841 acagccaata tggatagcca gctcaacgcc ctactaaaag aattcaagcc tggtcaatta
     3901 ttccgtaaat cagaccttac acaaattgaa gaagagatta aatctatttt aggggacgca
     3961 ggttatggtt cagctaaagt agatatttat cctcaattta atgaagaaaa taaaaccgta
     4021 aaaatcagtt ttgttgtgga tgccggtcgt cgtatttatg ttcgtaaaat ccgttttgaa
     4081 ggtaatggtg tcactgcaga ttcaacatta cgtcgtgaaa tgcgccaaca agaaggtgct
     4141 tggctttcca caaacaaagc ttctctaggt aaagctcgtt tagaaagaac cggtttctat
     4201 gaaagtgttg atatgtcaat gccaaatgta gaaggtacag ccgatcaagt tgatgtagtg
     4261 tataaaatta aagaacgcaa taccggtagt attaactttg gtatcgggta cggcacggaa
     4321 aacggtatca gctatcaagc aggaattaaa caagataatt tcttaggtat ggggtcaact
     4381 atcagtctaa atggtacccg taataactac ggtaccagcg ttaacctagg ctataccgaa
     4441 ccttacttta ctaaagatgg tgtaagttta ggcggtaata ttttctatga agattatgat
     4501 aactcagaca atgattcaat ggcttcatat aaacgccaaa cctatggtat taacggcaca
     4561 ttaggcttcc cggtaaatga aaataactca tactacttag gtttaggcta tacgcacgat
     4621 aaaattaaaa atgcagcacg tgagtacact cgtgagaaat atgtaaaatc aatgaacttt
     4681 gagttcgatc cagctgccga ttattacaaa aaaatcaaag ctgatgattt tgatttttct
     4741 ctcggttgga actacaacag cttgaataga ggctatttcc cgactgaagg aactaccgca
     4801 aacattggcg gtaaaatcac aattccaggc tctgataata aatattaccg agtcagtgca
     4861 gactttagaa attattaccc gttaaaccgt gagcacaaat gggtaatttc aacgaaagct
     4921 agcattgctt atactaatgg atttggtggt aaagaagttc cgttctatca gctttatact
     4981 gccggaggta ttggtacact aagaggtttc tcttacggtg cagtagggcc tcaagcgatt
     5041 tactataata caaaatccgg caaatttgat agcatgaata atgatattat cggtggtaat
     5101 gcgttagctg cggcaagctt ggaattaatc acaccaacgc catttgtgag cgaaaaatat
     5161 cagcacaatg taagaacctc tctctttgtt gatgctgcaa gtgcatggaa tacaaaatgg
     5221 aagaaagatg aatatacgaa cttgcctgac tatggtgatt tcaaacgttt ccgtgcttcc
     5281 gccggtattg cattccaatg gatgtctcca atcgggccat tatcattctc ttatgcgaaa
     5341 ccaattcgta aatatgataa tgatgatatt gaacaattcc agttcaacat tggtagttca
     5401 ttctaattaa ccacaagcgg ttaataaatg taaaaaattt gcaacttagc cgcttatttc
     5461 aaaactgttc atacaaggaa aacaaatgaa aaaattattt aaaatgactg gcttagccgt
     5521 agctttagcc tctacaaccg gaatcgcgaa tgctgcagat actattggtt tcgtagatcc
     5581 aagctatgta ttacaaaacc accctgtatt attagatgct tcggcaaaat ttgataaatt
     5641 catgaaagaa agccaagaaa aatttgctgc tgatgaaaaa aaattagcag atgaaaataa
     5701 aaccttaacg gctgagcgca ataaaattaa tgaagatgcg caaaaattac aaaatgaaca
     5761 aaaaaatgta gaagcttcta tcaagaaaaa agtggcagca ttagagaaag atgcgcctcg
     5821 cttacgttca aaagagattc aagcacgtca aaatgcgatt caaaaagaag caaacgcatt
     5881 ccaagctaaa gtaacagcaa ttcaaaaacg tgaagctgat tttgcaaaaa aagcagaagc
     5941 cttccagaaa cgtgcagatg aattccaaaa acgtattgaa caagcgaata aagaaaatgg
     6001 tggcattaac ccacaagaag cgcaaaaaca agcggttgat gaagtaaatg caaccattaa
     6061 agagatcgca aaatcgaaag gttacacttt agtattacaa ccacaagtag cactttatgc
     6121 tgaagatgaa ggtaaagata ttactgaagc agtacttgat gctattgtta aaaaacatcc
     6181 ggaaattaaa atagaaaaac ctgcaactga agctcaacca gctacagaaa aagaaaccaa
     6241 agctgaagaa actaaaccag aagaagcgaa aaaataatgg caaatttccg tttaattgat
     6301 ttagcggagc atatcggcgg tactcttagg ggtaacgccg atttggctat tcaaagtatt
     6361 gctgctttag ataaagccga cagctcgcaa attaccttta tctcaaatgc aaaatatcgg
     6421 gaaaatttga ccgcttgcaa tgctggagct attatcgtca gcccggcaga tgtagaattt
     6481 tgccgagcag atcaaaatct tatcatcgtt gccaatccgt atgttgctta tgctcaactg
     6541 gctcaatata tggattctac tccgaaagca gcgagcgaaa tccacccaag tgcggtgatt
     6601 tcacctgaag ctaaactggg taataacgta tcggtagggg caaatgccgt gattgaaagt
     6661 ggcgtagaat tgggcgatga tgccgtgatc ggtgcaggct gttttgtggg aaaaaacagt
     6721 aaaatcgggg caagaaccca actttgggca aatgtttcgg tgtaccacaa tgtacaaatc
     6781 ggagctgatt gcttgattca atcctcaacg gttatcggga gtgatgggtt tggctatgcg
     6841 aatgacaaag ggcaatggat taaaatccca caaaccggtg gtgtgattat cggtaatcgt
     6901 gttgaaattg gtgcttgcac ttgtattgac cgaggtgcat tagatccaac cgtaatcgaa
     6961 gataatgtga ttattgataa cctttgccaa atcgcccata atgtacacat tggttttggc
     7021 actgccgttg ccggcggcgt cattatggca ggcagtctaa aagttggtcg cttttgccaa
     7081 attggtggag caagtgttat caacggtcat atggaaatct gtgatggtgc cataattacc
     7141 ggaatgggta tggttatgcg cccaattaca gaaaaaggaa tttactcatc aggtattcca
     7201 ttacaaacca ataaagaatg gcgaaaaacc gcagcacttg taatgaatat tgatgaaatg
     7261 aacaaacgct taaaatcgtt agaaaaacgt tttaacgatt aattttatta gaggtcttaa
     7321 agtgacagaa atgacaactg aaaatcgtga gccaaatatt attgaagtaa ccgaaattat
     7381 ggatatgtta ccgcatcgct accctttcct gttagttgat cgcgtaaccg attatgaaga
     7441 agggaaatgg ttaaaagcca ttaaaaatgt aaccttcaat gaaccttgtt ttaccgggca
     7501 ttttcctggc gcaccaatct tcccaggagt attgattctg gaagcaatgg ctcaagcaac
     7561 tggcgtatta gccgttgcca cttatggtaa gttaagtgaa gacgaactct actattttgc
     7621 cgcaattgac aatgctcgct ttaagcgacc ggttgtgccg ggcgatcaac ttcaacttga
     7681 ggttgaattt cttaaagaag tacgtggtat tactaaattt accggtaaag catacgttga
     7741 tggcaaatta gtctgtgaag cagatttaat gtgcgctcgc cgtaaataat tcgatataag
     7801 cccaaactag agggaacaaa ctatgcgtct aattgattca acagcaaaaa ttagcccgct
     7861 tgccatcatt gaagaaggtg ctgaaattgg cgctcacgtt gaaattggtc cattttcagt
     7921 catcggtaaa gatgtcaaaa tcggggctag aacgaaaatt cactcaaatg ttgtcattaa
     7981 cggcatgact gaaattggtg aagataacca tattttccaa tttgccagca ttggtgaaat
     8041 caaccaagat ctaaaatacc aaggtgaacc aaccaaagtg gtgatcggta atcgtaaccg
     8101 tattcgtgaa agtgttacta ttcaccgagg tacggtacaa ggcggtggcg tgacgaaaat
     8161 cggtgatgat aacttattta tgatcaacac ccacatcgca cacgactgct ctatcggtaa
     8221 ccgttgtatt atcgccaata atggtacgct agcaggtcac gttaccctag atgatttcgt
     8281 gattgtggga ggaatgtccg ccattcacca attcgttgtg attggttcac acgttatgct
     8341 tggaggcggt tcaatggtca gccaagacgt accgccttat gtcatggcac aaggtaacca
     8401 cgcacgccca tttggtatta actttgaagg gttaaaacgt cgtggctttg ataaaccaac
     8461 tatgcacgct attcgcaaag tctataaact gatttactcc agcggaaaaa cattggaaga
     8521 gtgcctagta gaaattgagc aaattgcagc aacagaacct gcgattgcta tttttaaaca
     8581 attcttcaaa cgttctaccc gtggtattat tcgttaataa acgaaacccc gaaatcaatt
     8641 cggggtttgt tttatcaagc gaccgtcgaa cctttcaaca attttcgaat ccaagctcga
     8701 tttgggctaa ggttatgcca aatatcctca ctgattggca caggctcatt cacaatcagg
     8761 cttgctaaca attcccctag aagtggtgca gtcgtcaaac ctcgagaaga caggccgttt
     8821 accatataca gattggtaaa aatttcagca ttttctaccg cttgtttccg tctcaactgg
     8881 ttgtataaat tggcatattg gcttttttgc tgctcaaaat gatgcactgc cccaaccatc
     8941 ggtacacgat cacgaaacgc cgcacgaata ccgactttgg ctttattttc agataaatca
     9001 attccttgcg tccactcaca aacggttaca ttttgctgaa gttttgcaac attctcccaa
     9061 tgctccgata aactaaactc ggtttctgca ttatcccgaa tatggcttgc tccaatgcaa
     9121 tgcgtgccat aggctgacat tggcgttaaa taaccatcat aacaaagcac acacttcagt
     9181 ttttgtaatt cgcttgtagt cggaatttgg ctcacttgcc ctcgtaccgg atacaacgga
     9241 atgccgttcg cttggctaaa ttcattcaac gtatgtccat ttgccagcac aaccgcttga
     9301 tgtcgaaccg tttctcccct cgtctgccat tgccacattc cattagaaaa ggttaaatgc
     9361 tcaactttgt gattcagtac aatcttcaac cctttctgct ccagccaagt aaagccattt
     9421 ctaacaagtt gtaccggtga aagccaacca gcttgcggaa taaatgcccc gctgtttggc
     9481 acgcttaaac ctaatttttc acttaattct gcctgtgaac aagattttaa taaggtgtca
     9541 tcagccgtct gctccaccat tttttccagt tttttagccg ttttctcgtc ataggcgtaa
     9601 agcactaccc cgcttaaatt atgctcaaac tcaatgttgg atttcagttg ttgcaagcgt
     9661 tgcaatgcat aatcaaaact atggatataa aactgtacat ttcgctcgtc atcatcgctt
     9721 aactgtgggt aaatcgcccc ttgtaaattt cctgaagcat ttattgccaa ggtttcatct
     9781 ttgcaataca gcgtgacttg cttgcctttt tctaacagcg ataacgcaac gaagaaactc
     9841 gctactccgc caccaataat tgcaatgtca tcagcggttt catttttggc ataaaaatag
     9901 gggtaattag gctcggaaac ttggctatct aacggtttct cgccccaaag catttctcgt
     9961 tttttgccaa atcctttgcg ttttttcact gcaaaacctg cggcttccag ccctcgtctg
    10021 accatacttg aagcggtaaa agtagcaaaa ctgccgccat tggcacttag tcgaaacatt
    10081 tgcagatata actgttcgtt ccacatttct gggtttttat caggcgaaaa gccatctaaa
    10141 aaccaagcgt caatcgtgcc gctataaaga tcgcctaatt ggggtaagtt ttcagcaatc
    10201 tcaccgaacc ataaatctaa ataaacctgc tcaaaatgat aacgttggca acctatttgg
    10261 cgaggctgcc aacaagcggt taaattttca gataatgttg caaattgcgg atagcgttgg
    10321 tgaatatcag ctaattggct ggccgtcaga ggaaatttct caaatgagat aaaatagagt
    10381 cgttttagtt tactttgcgg aaatgctgcc cgaaactgtt ggaatttgtc agcaacggct
    10441 aaaaaattca agcccgtgcc aaagccggtt tccgcaataa caaaagcctc tttcgggtga
    10501 gattgccact tttcccacag ttggttacct tcttgaaaca catagtaact ttccgccaaa
    10561 ccatcttggg tagaaaaata aatatcatca aattgctccg acaccggtgt atctgcatca
    10621 ttaaatgtaa gtgtcgcaaa agtcagttta cccatcatat ccccttaaaa acaaaacacg
    10681 gaaaacccca agattttccg tgtttcaacc agttcaatag taccaatgtt agaaaatgtt
    10741 gctggcaaaa ataacgatca taccaagtgc gcctacaacc gttagcattg aataaccaaa
    10801 agtacccatt ttacaatcct cattgttaat tatgattaaa acggctttat tctagcataa
    10861 tttccatcgc tttgaatgcc tatgcttgaa taattttgcc aaaacaagcg gtcaattttt
    10921 gtaaatcttt tacgcctttg gcactttcca cgccagaatt taaatctaac ccgacacagc
    10981 cttgagcaag ggcttgctca atattctcag tgccaatacc acctgccaat aacgcttttt
    11041 gttttaaggt ttctggaatc aacgcccaat taaaggtttt gcctgtgccg ccttgctgat
    11101 tgcctacttt gctgtctaat acataacgag caatctgtgg attatcgtta aattctagat
    11161 tttcaacctc aacatcaacc gataaggctt tccagatttg agttttacta cctaatttct
    11221 ctgccagttc tgcaatgtat tgctcatctt ccgagccgtg caattgaact gcaaataact
    11281 caagctgttt tgccattttt tccacaaatt cgaccgcttg attttggaac acccccacat
    11341 aacgcaacgg tgcattcacc accaattctt gggcttggcg aagtgaaagc tggcgaggcg
    11401 agccttcggc aaaaattaaa ccgccatata atgccccttg ctcataaacc gctttcacat
    11461 cttgcacacg ggttaagcca cacactttgt tttcaccaaa aatcaccgcc cgcaccgcat
    11521 tattgagatc ggcacttccc attaaactgc tgccgattaa aaaagcatga acatacggct
    11581 taatgttttt cacttgctgg tgatcgttaa tacccgattc tgaaatcaaa cagacatcag
    11641 ccgaaatttg ggcttggtat ttttccacca aacgcacaat gcggttcata tcaatcgtga
    11701 gcgtgtgcaa atcacggtta ttaacgccaa ttaccttcgc ccctaaggct aaggcacgtt
    11761 caaattccgc ctccgtgctg gtttcagtca aaacgcccat tcctaactcg tgagccagtg
    11821 tggcaagttc acggtaggtt tcatcattta ccaccgataa catcaataaa atcgcatcag
    11881 cattataaaa acgggcaagg taaacctggt atgccgaaat cataaaatct ttacacaaaa
    11941 tcggctgtgt ggtttggcgt ttgacttgat cgatataacg gaaatcgcct tgaaaatatt
    12001 gctcatcggt taaaaccgaa atcgccgcag cgtaattttt atacacttgg gcaatactgt
    12061 ctaaatcgaa ctcagcacga attaaccctt ttgacggtga agctttttta cattctaaaa
    12121 tataagctgg ttcttggtgt gtgcctttgg caagagccgc atagaaatca tggtcgcttt
    12181 ttacaatttt gtgctgaaat tcacttaacg gaaaggcttg ctctttttgt gccacccaaa
    12241 tggctttatc ttgtacgatc ttttgtaaaa tcgtcggttg gtttggttga gcgttatctt
    12301 gaacgatgtt ttgcatataa attcctagag cgaaatcggt ttgaatggta aaatctgccc
    12361 ttatttttac ccgaaaaagt ctcatttcac aagcaaaact aagatgaaat cacaagttat
    12421 acagcattta tcttatttag aaatcgccct gagagttaat caactttggg aggcgacacc
    12481 accaagccct gaagccttag ccaatgagca acctttctgc gtggaaacct taaccccaac
    12541 ccaatggcta caatggattt ttattcccag aatgcgggcc attttagatg caaatgccga
    12601 tttacctcgc aattttgcaa tcacccctta tttagaagag gcattgaaaa acgaaggtta
    12661 tttacaagcg atttgtgaac cgctattagg tttggaaaag ttgttgaaag attaacttct
    12721 ctctcagttc ttttattaat ctgacaaatt cgttgatagt gccagcgtcc acgctggtac
    12781 taattttaac ggttaattaa tcaaaatttg gcacaagcga gga
//
LOCUS       DANLRK010000071        12770 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.071, whole genome shotgun sequence.
ACCESSION   DANLRK010000071 DANLRK010000000
VERSION     DANLRK010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 12770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12770
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.071"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            24..248
                     /locus_tag="PXA33_001395"
     CDS             24..248
                     /locus_tag="PXA33_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HDL5120719.1"
                     /translation="MVVKILKRKSQKTLQQIYTQPISANIKWTNIEALFLELGAEIEE
                     REGSRIAVVLFQQVRVFHRPHPNPDTDKGR"
     gene            291..620
                     /locus_tag="PXA33_001396"
     CDS             291..620
                     /locus_tag="PXA33_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HDL5120720.1"
                     /translation="MRNIMDINGYKAVIAYDPETELFRGEFIGLNGGADFYADNVIQL
                     KKEGELSLNIFLELCREKGIEPFKQYSGKFNVRLSPELHKAAVIAATAENLSLNEWIN
                     QTLEKSV"
     gene            complement(659..1045)
                     /gene="cueR"
                     /locus_tag="PXA33_001397"
     CDS             complement(659..1045)
                     /gene="cueR"
                     /locus_tag="PXA33_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005654127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HDL5120721.1"
                     /translation="MNISQAAEKTGLSAKQIRDYEKAGLLPPTSRTEAGYRFYNATDL
                     SRLHFISNARKVGFSLAQISELLKLNDNPNRSSRDVKQLTEQHIGELEQKIADLNQML
                     TLLKSWSSSCCGNANSECSILNGLNG"
     gene            1134..1334
                     /locus_tag="PXA33_001398"
     CDS             1134..1334
                     /locus_tag="PXA33_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HDL5120722.1"
                     /translation="MTITLQLDGLHCGNCVKSVEKALNGVAGVNQVSVTLEPQIAVVE
                     GSAEAQALIEAVEDIGFDAKLA"
     gene            1387..3579
                     /locus_tag="PXA33_001399"
     CDS             1387..3579
                     /locus_tag="PXA33_001399"
                     /EC_number="3.6.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005710411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HDL5120723.1"
                     /translation="MTSTAHYSPEQQLLIDGMHCAACVRRVEKALLKVEKVEFASVNL
                     ADQTAFVQGNANPEALVAAVVKIGFGAEVLESEEERRIKQQAQTQRVLGHKRTQFMLA
                     LVVGFALVAFGFYKGMTVNETNRSLWLSWAGISLATMYFSGKDFFLGAFSALKNRSAN
                     MDMLVAISTGTAWLYSCWLTLFPQPNAHVYFEASVMILGFINLGKYLELKAKQRSSLA
                     LEKLLDLAPKQAVIFEENIAKTIPAKAIKPQMRVQALTGDRLAVDGILDSGSIWVDES
                     MLTGEALPVEKKIGDKVRAGTLVQDGSGTYIAEQVGSQTALARVINAVRHAQSSKPPL
                     AQFVDKIAAVFVPVVVSIALLSALIWLAVGQDFAFALSIFTTVLIIACPCALGLAIPL
                     STIAGVARSAEFGVLVRNIEALQASSEIDTLVFDKTGTLTTGRMEVSDVLTFNGFEQK
                     RLLQLAKSVEQHASHPIAKAIAKFAENQTACGVTDVQVLKGLGISAKYEGSTIRMGNW
                     QFVGETIQANLASLLPAKEGDFSSKIFVSVDNQLAGVLSVSDQLRPEAKATIHQFQAE
                     GYQCVMLTGDRQQTADYFANALGLDGVIAEVLPEQKAEKIRALQAEGKKVAMIGDGIN
                     DAPALAQANVGIAMHNGSDIAVETADLSLMREGLAPLMQILPFSKKVLKNMKQSLLGA
                     FIYNVIAIPIAAGVLYPFTGWLLNPMIAAIAMTLSSITVVLNSQRLLK"
     gene            complement(3632..4417)
                     /gene="truA"
                     /locus_tag="PXA33_001400"
     CDS             complement(3632..4417)
                     /gene="truA"
                     /locus_tag="PXA33_001400"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDL5120724.1"
                     /translation="MKIALGIEYDGSRYFGWQRQEAVESVQQQLEQALSVVANSPVEV
                     FCAGRTDAGVHGTGQVVHFETQANRPLQSWCFGTNAHLPDDIAVKWAVAVNEDFHARF
                     SATARRYRYIIFNQKLRSAILPKGVTHFHFPLDERKMHQAGQYLLGENDFSSFRAAKC
                     QSNTPWRNIHHLNVSRQGNYVIVDIQANAFVHHMVRNIVGTLLEIGQGRQPVEWAKWV
                     LEQRDREKAAPTAKAEGLYLVEVHYPEHFGIPKTPLGPLFLAD"
     gene            complement(4482..5171)
                     /gene="folE"
                     /locus_tag="PXA33_001401"
     CDS             complement(4482..5171)
                     /gene="folE"
                     /locus_tag="PXA33_001401"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HDL5120725.1"
                     /translation="MTTISPELTITENTLSPEAQKVRQALLAKGIETPTVTHTKDKAS
                     RRAEIQQHMQAVLELLGLDLSDDSLEETPHRLAKMYVDEIFSGLDYATFPKITNIENR
                     MKVSEMVLVDDITLTSTCEHHFVTIDGKVAVAYYPKKWVIGLSKINRVVQFFAQRPQV
                     QERFTEQILTAFQTILETDDVAVYVKATHFCVKCRGIKDTNSYTVTSAFGGVFLEDRE
                     TRKEFLGLLNK"
     gene            5272..5979
                     /locus_tag="PXA33_001402"
     CDS             5272..5979
                     /locus_tag="PXA33_001402"
                     /EC_number="2.7.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid kinase"
                     /protein_id="HDL5120726.1"
                     /translation="MQNIQYLFNADLIPEHQAEFVQSLLTCKNFDNSDRLLGFSKGRG
                     TTWFLDTSAELGINVVLRHYYRGGLFGKIIKDSYFFTRFEATRAYKEFFLLQQMLAWG
                     LPVPRPVAIKIERHFCCYRADIMLEKLENTQDLSKILQSHSLSNEQYEQIGKLIRSLH
                     NHQIHHSDLNIHNILLGNDGKFCLIDFDKCGIEPGENWKQNNLDRLLRSFKKEQGRLN
                     IFFSEENWQAVLRGYTL"
     gene            complement(6034..7131)
                     /gene="apbC"
                     /locus_tag="PXA33_001403"
     CDS             complement(6034..7131)
                     /gene="apbC"
                     /locus_tag="PXA33_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HDL5120727.1"
                     /translation="MNQLNEQQLVEIKAILQQFSHPTLQKDLIALNAFKKAELGGDIL
                     RIEITMPFAWNSGFETLKAETEAKLKQVTGSSGVKWVLNYQIATLKRANKHPAVNGVK
                     NIIAVTSGKGGVGKSTTSVNLALALRAQGTRVGILDADIYGPSIPHMLGAKDQRPTSP
                     DNKHITPITAHGLQSNSIGYLMEEDNATIWRGPMASSALSQLLNETWWNELDYLVIDM
                     PPGTGDIQLTLSQQIPVTGAVVVTTPQDIALLDAIKGISMFEKVSVPVLGVIENMSMH
                     ICSNCGHHEAIFGTGGAEKIAQKYGTKVLAQLPLHIRLREDLDSGVPTVVAAPEHEIS
                     QAYLALAEKVASELYWQGSVIPSEIMIREVK"
     gene            complement(7128..8675)
                     /locus_tag="PXA33_001404"
     CDS             complement(7128..8675)
                     /locus_tag="PXA33_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL5120728.1"
                     /translation="MLKSLQAVTFVKKIAKVTACILLITSCDQLICPQSIQSVWVENR
                     QSDTLLKRAIYSAVFQLDPHKIKASADAAPLRDLLVGLTAYNRKGDVVPAIAQNWFTE
                     DEKEWLFILDEKAVWSNGQPVTAQDFVASWRRLIDPQNHSVLSKYLIYMGIENAKAIE
                     EQIKSVEELGVEALNAHTLRIRLNHPNHQLPEMLAHSALLPTYQGIAPTIDNFVSNAS
                     YRLEKLDAQKMVLKARTQEIPFQTIEYDLITTIQNCSAFDIVENPLLNKKTNIVKFPR
                     LCSYYYEFNFDDPLLAKREIREAIRTMVASANSGQEYGVPSQSVIPHSLVKDREKRWN
                     PVVVEQLLTKAGISPYNPLKLTLLYDENSINIANQVTRALSQSELFNITPKMVDWQTL
                     NKVREQKDYQIARAGWCADYPDPIVFLQKFHSMSPDNHSGYKSEIVDQKLNRLQTETL
                     SAEARESLIQEISEQLYSDVAVLPLFQYHHRVGIVSSLLGIDLNNDSEVIYSKDLKRN
                     LANKDNQ"
     gene            complement(8701..9351)
                     /locus_tag="PXA33_001405"
     CDS             complement(8701..9351)
                     /locus_tag="PXA33_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchE family NAAT transporter"
                     /protein_id="HDL5120729.1"
                     /translation="MDIIINFAIYLQFFIGLFAIVNPFGSLPIFFSMTAHQYEGERNR
                     TSLITAVSIGVILLVSLYFGNLILNAFSISLSSFRVAGGILIVSIAMTMISGKLGEHK
                     QNKEEKNEDITEYENIGVVPLAMPIMAGPGAIGSTIVWGTRYHTWEDYVGFSIAIIVF
                     ALICYILFRYSAPLVKKLGKTGSNVVTRIMGLILMALGIEIIVAGITTLFPVLLVK"
     gene            complement(9473..11344)
                     /locus_tag="PXA33_001406"
     CDS             complement(9473..11344)
                     /locus_tag="PXA33_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HDL5120730.1"
                     /translation="MIEKMHERTNGPVFKIIFALVSISFVIGGIGTGLISQDNSVAKV
                     NGEEISQQLFNNTLNREQNRLNAELGSRFWDLMDSPEYAAQFNQSVLNGLIDEELLRQ
                     YAKELKLGISADQIKSEIVNSQMFQQDGKFSNELYQQTLRHNNLSADGYAAIVQEGML
                     QSQLQEGIINSDFNVPAQQALLAKLLLQQREVRLAEFSVAKEMENQTASEQELRAYYE
                     ANKTKLLAPEKLAVEYVTLSPKDVESKVEITNEQIQTYYDRNKADYVTKGEAHLAHIQ
                     VASEAEAQAIEQALKNGEDFATLAKNKSSDTLSANNGGDLGWAKAGTFPKAFEDAVAN
                     LTAGQMSAAVKVDNAYHIIKVLERKPEQVIELAQVKDKIAQTIRQELVLTEYFTVARE
                     MANKAFENNSSLEEVAKAGNVELQKTDLFTRENVPTALQNEKVLKVLFEGELRQNKQN
                     SDALDVGNDANPQTLFVRVSDYDAERPQTFDEAKAAVENFVKAEKAEKALLAKVEENV
                     KALNEGKSVDVKFNAPQTLVYMQAEVENPVLAGTIFAMKKAQDKTTYQMARNQKGDVV
                     IVALDNITEGNAEQFKTLAPQFEQANRLILRNEFLQSLRAKASIEVNEDFMQQRSSH"
     gene            11526..11795
                     /gene="rpsO"
                     /locus_tag="PXA33_001407"
     CDS             11526..11795
                     /gene="rpsO"
                     /locus_tag="PXA33_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HDL5120731.1"
                     /translation="MSLSVEAKAKIVAEFGRDAKDTGSSEVQIALLTAQINHLQAHFA
                     EHKKDHHGRRGLLRMVSRRRKLLDYLKRTDLAKYSETIARLGLRR"
     gene            11867..12736
                     /locus_tag="PXA33_001408"
     CDS             11867..12736
                     /locus_tag="PXA33_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HDL5120732.1"
                     /translation="MAFASLFTLLDDIAAVLDDVSIMTKVAAKKTAGVIGDDLALNAN
                     QVSGKNVVPERELPIVWAVAKGSLINKVILIPLALLLSVFLPQAIIPLLMIGGAYLCF
                     EGVEKLLHRLLHKNNQAHSDETETLSEKDKIKGAIRTDFILSAEIIIIALGVLQDSST
                     LTQILSLSAIGVGITLFVYGLVGLIVKLDDIGLWLMQKKSTFSQAVGKGLLFAMPWFM
                     KGLSVIGTIAMFLVGGGIFTHNLPMIHHFVEQFGISPHFSTLVDFAVGIVVGIIACLI
                     ILPLMKLFSKKAH"
BASE COUNT         3656 a         2688 c         2528 g         3898 t
ORIGIN      
        1 ttattttaaa atggtactat attatggtag taaaaatcct aaagcggaaa tctcaaaaga
       61 cactacaaca aatctacacc cagcctattt ctgccaatat taaatggaca aatatagaag
      121 ctctttttct agagttagga gcagaaatag aagaacgaga agggagcaga attgcagttg
      181 ttttatttca acaggtcaga gtatttcatc gcccacaccc gaatcctgat acagataagg
      241 ggcggtagcg agtattaaaa aatggttatc tgaaaacgga gtcattcctt atgcgtaata
      301 ttatggatat taatggttat aaagctgtca ttgcttacga tcccgaaaca gaattatttc
      361 gaggtgaatt tatcggatta aatggtggag ccgattttta tgccgataat gtcattcaat
      421 taaaaaaaga aggtgaacta agtttgaata tctttttaga actttgtaga gaaaaaggta
      481 ttgagccgtt caaacaatat tccggaaagt ttaatgttcg cctttcgcct gaattacata
      541 aagctgcggt aattgcagca actgctgaaa atttaagctt aaatgaatgg attaaccaaa
      601 cgttagaaaa gagcgtgtag cctaattgca aaacaatcac ctattttgac cgcttgtatt
      661 acccgtttaa cccattcaat atcgaacact ccgaattggc gttgccacag caagagctac
      721 tccagctttt gagcaaagtc agcatttggt ttaaatcggc aattttttgt tcaagctcac
      781 cgatatgctg ttcggttaat tgttttacat ctcggcttga gcggtttggg ttgtcgttta
      841 atttaagtag ttcgctgatt tgagccagcg aaaagcccac ttttcgggca ttgctgataa
      901 aatgtaatcg gctcaaatca gtagcattgt agaatcgata ccccgcttcg gtgcgacttg
      961 tcggcggcag aagtcctgct ttttcataat ctcggatttg tttggcggaa agcccggttt
     1021 tttctgcggc ttggctaata ttcattataa tcctctcact ttaatcgctt gaccttaacc
     1081 taaggttaag ctttattata cgcctgaata atcactcaaa acaggagaaa atgatgacaa
     1141 ttaccctaca acttgatggc ttacattgtg gcaactgtgt gaaaagtgtc gaaaaagcgt
     1201 taaatggcgt ggctggggtg aaccaagtta gcgtaacgtt agaaccgcaa attgctgtgg
     1261 tggaaggctc agcagaagca caagccttaa ttgaagcggt tgaagacatt ggctttgacg
     1321 caaaattagc ctaattaata aggggcagaa attttgccct aatctgccca atatttcgga
     1381 ggataaatga caagcacagc tcactattcg ccagaacaac aacttttgat tgatggaatg
     1441 cactgtgccg cctgtgttcg gcgagtggaa aaagccttac ttaaagtgga aaaggtggaa
     1501 tttgcctcag tcaatcttgc cgatcaaacc gcttttgtgc aaggcaacgc caaccctgaa
     1561 gccttggtag cagcggtagt taaaatcggt tttggggcag aagtgctgga aagcgaggaa
     1621 gaacgccgaa ttaaacaaca agcccaaacg caacgagtgt tagggcataa acgcactcaa
     1681 tttatgcttg cccttgtagt tggttttgcc ttagttgctt ttgggttcta caaaggaatg
     1741 acggtaaacg aaaccaatcg ttcgctttgg ctcagctggg caggtattag ccttgcgaca
     1801 atgtattttt caggtaaaga tttctttctt ggggcatttt cagcactgaa aaaccgctca
     1861 gccaatatgg acatgttagt agcaatcagc accggcacgg cttggctcta ttcttgttgg
     1921 ctcacgcttt tcccacaacc gaatgctcac gtttattttg aagccagcgt gatgattctc
     1981 ggctttatca atctaggaaa atacttagaa ctgaaagcca aacagcgttc ctcgctagca
     2041 ttggaaaaat tgctggatct cgccccaaaa caagcggtca tttttgaaga aaatattgca
     2101 aaaaccattc cggctaaagc aattaagcca caaatgcggg tgcaagcctt aaccggtgac
     2161 cgcttggcgg ttgatggtat tttagacagt ggctctattt gggttgatga atcaatgctc
     2221 accggtgaag ccctacctgt agagaaaaaa atcggtgata aagtgcgagc cggcactctg
     2281 gtgcaagacg gttcaggcac ttacattgcc gaacaggtcg gctcacaaac cgctttggct
     2341 cgtgtaatca atgccgttcg ccacgcccaa agcagcaaac cgccattggc acagtttgta
     2401 gataaaatcg ccgctgtttt tgtgccggtg gtggtttcaa tcgcattact ttctgcgtta
     2461 atttggctgg cagttgggca ggattttgcc tttgcactct cgatttttac taccgtgttg
     2521 atcatcgctt gcccttgtgc attggggctg gcaattccgc tttctaccat tgccggcgtg
     2581 gcacgttctg ccgaatttgg cgtgctggtg cgaaatattg aggcactaca agcaagcagt
     2641 gaaatcgaca ccctagtgtt cgataaaacc ggcacactca ccactggcag aatggaagtg
     2701 tcggatgttc tcacctttaa cggatttgag caaaagcgct tgctacaact tgctaaaagt
     2761 gtagagcaac acgcctcaca cccgattgcg aaagcgattg caaaatttgc cgaaaatcag
     2821 accgcttgtg gtgtaaccga tgtgcaagtg ttgaaagggt taggcatttc agcaaaatat
     2881 gaaggcagta caatcagaat gggaaattgg caatttgtag gcgaaaccat tcaagcaaat
     2941 cttgcctccc tattgcccgc taaagagggg gatttttcct caaaaatttt tgtttcggtg
     3001 gataatcaac tcgccggtgt attgtcggtt tcagaccaac ttcgccctga agcgaaagca
     3061 accattcatc aattccaagc ggaaggctat caatgcgtga tgctgaccgg tgatcgccaa
     3121 caaactgccg actatttcgc caacgcattg gggttagacg gcgtgattgc ggaggtatta
     3181 ccggagcaaa aagcggaaaa aattcgagcc ttgcaagcag agggtaaaaa agtagcaatg
     3241 attggcgacg gcataaacga tgctccagct ctggctcaag ccaatgtggg cattgcgatg
     3301 cacaatggtt cagatattgc agtggaaacc gccgatcttt cgctgatgcg agaagggcta
     3361 gcaccactga tgcaaattct accgttctct aaaaaagtgc tgaaaaatat gaagcaaagt
     3421 ttgctcgggg cttttatcta taatgttatt gcgattccta ttgctgccgg cgtgctttac
     3481 cctttcaccg gctggctact caacccaatg attgccgcca ttgcgatgac attgtcgtca
     3541 attaccgtgg tgttaaatag tcaaaggttg ttgaaataat ttaagcggtc aaatttgcat
     3601 atttttttgc aaatttgacc gcttgtaatc actaatccgc caaaaacagc ggcccaagag
     3661 gtgttttcgg aatgccaaaa tgctcagggt aatgcacctc gaccaaatat agcccctcgg
     3721 ctttcgctgt aggggcagct ttttcccggt cacgttgctc caacacccat tttgcccatt
     3781 ccacaggctg tctgccttgc ccgatttcga gcaatgttcc cacaatattg cgaaccatat
     3841 gatgtacaaa ggcgttggct tgaatatcaa caatcacata attgccttgc cgagacacat
     3901 ttaaatgatg aatattccgc cacggcgtat tagattggca tttggcggca cggaaagagg
     3961 aaaagtcatt ctcgcccagc aaatactgcc ctgcttggtg cattttgcgt tcatcaaggg
     4021 gaaagtgaaa gtgggtaaca cctttaggta aaattgccga ccgcagtttt tggttaaaaa
     4081 taatatagcg gtaacggcgg gcggtggcac taaaacgggc gtgaaaatcc tcattcaccg
     4141 ctaccgccca tttcaccgca atatcatcgg gtaaatgagc gttagtgccg aaacaccagc
     4201 tttgtagcgg gcggtttgct tgcgtttcaa aatgcacgac ctgccctgtg ccgtgcacac
     4261 cggcatcggt tctgccggca caaaacactt ctacgggcga attggcaaca accgacaacg
     4321 cctgctctag ctgttgttgt acactctcta ccgcttcttg tcgttgccag ccaaaataac
     4381 ggctgccgtc atattcaata cctaatgcga ttttcatttt aataccttga taaaaagagg
     4441 ggcgaaaaat ttttcgcccc cacagtaatt gatatttgca attatttatt caataagcct
     4501 aaaaattctt tgcgggtttc tcggtcttct aagaaaacac cgccaaaggc agaagtaact
     4561 gtatagctat tggtgtcttt tatgccacgg catttcacgc aaaaatgggt ggctttcaca
     4621 tacaccgcaa catcgtctgt ttctaaaata gtttgaaagg cagttaaaat ctgttcggta
     4681 aaacgctctt gcacctgcgg acgttgggca aaaaactgca cgacacggtt gattttcgag
     4741 agtccaatca cccatttttt cgggtaatag gctaccgcta ctttgccgtc aatagtaaca
     4801 aaatgatgct cacaggtaga agttaaagta atatcgtcca ccagcaccat ttcactgact
     4861 ttcattcggt tttcaatatt ggtaattttc gggaaggtag cataatcaag cccgctgaaa
     4921 atttcatcaa catacatttt cgccaaacga tgaggcgttt cttctaagct gtcatcactt
     4981 aaatctaaac cgagcaattc taaaactgcc tgcatatgct gctggatttc tgctcggcga
     5041 ctggctttat ctttggtatg agtaacagtc ggagtttcaa tgccttttgc caacaaggct
     5101 tggcgaactt tttgagcctc cggcgaaagc gtgttttctg tgatagttaa ttcgggcgag
     5161 atcgttgtca ttggttttcc tctcaaatgt aagctgaaga ttctaacaaa ttgacatcaa
     5221 aaaaaatagc tctaaaatga gctatttcag ccataaacac cctctttatt tatgcaaaat
     5281 atacagtatc tctttaatgc cgacctgatt cccgaacatc aagctgaatt tgtgcagtct
     5341 ctcttaactt gcaaaaattt tgacaattcc gaccgcttgc taggcttctc aaaagggcga
     5401 ggtacgactt ggtttttaga tacctctgct gaactaggta tcaatgtggt gcttcgccat
     5461 tattatcgtg gcggcttgtt cggtaaaatc atcaaagata gttacttttt tactcgcttt
     5521 gaggccaccc gagcgtataa agaatttttc ttactgcaac aaatgttagc ttggggttta
     5581 cctgtgcctc gccctgtggc gattaaaata gaaagacatt tttgttgcta tcgtgccgac
     5641 attatgctcg aaaagctaga aaacacacaa gatttaagca aaatcttgca atcccattct
     5701 ttatcgaacg aacaatatga gcagataggg aaactgattc gcagtctgca taaccatcaa
     5761 attcaccatt ccgacttaaa tatccataat attttgcttg gtaatgatgg caaattttgt
     5821 ctgattgatt ttgataaatg cggcattgag ccgggcgaaa attggaagca aaataaccta
     5881 gaccgcctgt tacgctcgtt taagaaagaa caagggcgat taaatatttt cttcagcgaa
     5941 gaaaattggc aggctgtttt gagaggatat actctctaag aaacaataat ggcacaagct
     6001 tagaaacttg tgccatcaaa tcatcaatat taactacttc acttcacgaa tcataatttc
     6061 agacggaatc acactgcctt gccagtaaag ttcagaagct actttttccg ctaatgcgag
     6121 gtaggcttgg ctgatttcgt gttccggagc tgccaccacc gttggcacac cgctatctaa
     6181 atcttcacgc agacgaatat gtagtggtaa ttgtgctaac actttagtgc catatttttg
     6241 ggcgattttt tccgctccgc cggtgccgaa aattgcctca tggtgtccgc agtttgagca
     6301 gatatgcatt gacatatttt caatcacccc aagcactggt actgagactt tctcaaacat
     6361 tgagatccct ttaatcgcat ctaataaggc aatatcttgt ggggttgtta ctaccaccgc
     6421 accggtcacc ggaatttgct gtgaaagcgt aagctgaata tcacccgtac ccggtggcat
     6481 atcaatgacc aagtaatcca attcattcca ccaagtttca tttaaaagct gacttaacgc
     6541 actgcttgcc atcgggccac gccaaatggt ggcgttatct tcttccatta aatagccgat
     6601 agaattagat tgcaagccgt gagcggtaat cggcgtgatg tgcttgttat caggagaagt
     6661 tggacgttgg tcttttgctc ccaacatatg cggaatagat gggccgtaaa tatccgcatc
     6721 taaaatcccc actcttgtcc cttgtgctcg taaggctaaa gcaagattaa ctgaggtcgt
     6781 tgatttccct accccacctt ttcctgaagt gaccgcaatg atatttttta ccccattgac
     6841 tgccgggtgt ttattcgccc gttttaaggt cgcaatttgg taatttaata cccattttac
     6901 accgcttgaa cctgttactt gctttaattt agcctctgtt tcggctttta aggtttcaaa
     6961 accactgttc cacgcaaatg gcatcgtgat ttcaattcta agaatatcgc cccctaattc
     7021 tgctttttta aacgcattaa gggcaattaa atctttctgt aaagtcgggt gggaaaattg
     7081 ttgcaagatt gctttaattt caacaagttg ctgttcattt aattggttca ttggttatcc
     7141 ttgtttgcta aattgcgttt taaatctttg ctataaatta cttcgctatc gttattcaaa
     7201 tcaataccca aaagggagga aacaatccca acccgatgat ggtattgaaa taatggcaat
     7261 acggcaacat cgctataaag ctgctcgcta atttcttgaa taagtgactc tctcgcttct
     7321 gcactaagcg tttctgtttg taaacgattt aatttttgat cgactatctc acttttatag
     7381 ccggaatgat tatccgggct cattgaatga aacttctgca aaaatacaat cggatcagga
     7441 taatctgcac accagcctgc tcgtgctatc tggtaatctt tttgttccct tactttattt
     7501 aaagtctgcc aatcaaccat tttaggtgta atattaaata attctgattg agaaagggct
     7561 cgagtaactt ggttagcaat attaatgcta ttctcatcat ataacaaagt cagctttaaa
     7621 ggattataag ggctaattcc tgcttttgtt aatagctgct caaccacaac aggattccaa
     7681 cgcttttctc tatcttttac taagctatgt ggaattacag actgactcgg aaccccatat
     7741 tcttgcccac tatttgctga agccaccatt gtccttattg cttcacgaat ctctcgttta
     7801 gctaataatg gatcatcaaa attgaactca tagtaataag agcacaaacg cggaaatttc
     7861 acgatattcg tttttttatt taataaagga ttctcgacaa tatcaaaagc agagcagttt
     7921 tggatagttg taattaaatc atactctata gtttgaaagg gaatttcttg ggttcgagcc
     7981 tttaacacca ttttttgagc atctagtttc tcgagacggt aagacgcatt gctcacgaag
     8041 ttatctatgg ttggagcaat cccttgataa gtaggtaata atgcggaatg tgccagcatt
     8101 tcaggcaatt ggtgatttgg gtgatttagc cgaattctca atgtatgcgc atttaatgcc
     8161 tctacaccga gttcttcaac tgactttatt tgctcttcaa tcgcttttgc attttctata
     8221 cccatataga tgagatattt agacaataca gaatgatttt gaggatctat taaacgtcgc
     8281 cagcttgcta caaaatcttg tgcagtcaca ggttggccat tagaccacac agctttctca
     8341 tctaaaataa acaaccactc tttttcatct tcagtaaacc aattctgagc aatggccgga
     8401 acgacatcac cttttctgtt gtaggccgtt aaaccgacta atagatctct taaaggagca
     8461 gcatctgcag aagctttaat tttatgtggg tcgagttgaa atacagcaga ataaattgca
     8521 cgtttgagta aagtatcact ctgccggttt tctacccaga cagattgaat ggattgaggg
     8581 caaattaatt gatcacaaga tgtaataagc agaatgcaag cggtaacttt tgcaattttt
     8641 tttacaaatg taaccgcttg taaggatttc aacataaact ccccaaaggt taattaagat
     8701 ttattttaca agaagcacag gaaataaagt tgtaattcct gccacaataa tttcaattcc
     8761 cagcgccatt aaaatcagcc ccatgatacg agtaacgaca ttagatcctg ttttgcctaa
     8821 ttttttcacc aaaggggctg aatagcggaa taaaatataa caaattaatg caaatacaat
     8881 aatcgcaatg ctaaagccaa catagtcttc ccaagtatgg tagcgggttc cccacacaat
     8941 ggttgagcca attgcccctg gtcctgccat aatcggcatt gctaatggca cgacaccgat
     9001 attttcgtat tcagtaatat cttcattttt ctcttcttta ttttgtttgt gttcacctaa
     9061 tttaccgcta atcatcgtca tcgcaatgct tacaatcaaa atgccaccag ctactctaaa
     9121 agagctaagt gaaatgctaa acgcattgag aatcagatta ccaaaataga ggctgactaa
     9181 taaaattact ccaatagaaa cagcggtgat taagctggtg cgatttcttt ccccctcata
     9241 ttggtgagcg gtcatactga aaaaaatagg caaggaacca aaaggattga ctatagcaaa
     9301 tagaccaatg aagaattgga ggtagatggc aaaattaata atgatatcca caaaaacttt
     9361 ctcaaacaac agtgtttcgg gcgctattat atcggttaat caaggtagat acaaaccaaa
     9421 agcacccagc ctaaaggacg gttgggtgct ttttgataaa atgaaaggct tgttagtgtg
     9481 acgaacgttg ctgcataaaa tcttcattta cctcaattga agcttttgca cgtagcgatt
     9541 gtaagaattc attacgtaaa attaaacgat tagcttgttc aaattgtggc gccaatgtct
     9601 taaactgttc agcattccct tccgtaatgt tatctaaggc aacaataact acatcgccct
     9661 tttgattacg tgccatttga taagtcgttt tatcttgcgc ctttttcatt gcgaaaatcg
     9721 ttccagccaa taccgggttt tctacttctg cttgcatata aactaaagtt tgtggcgcat
     9781 taaatttcac gtccactgat ttcccttcat tcaaggcttt tacattttct tctactttcg
     9841 ccagcaacgc tttttccgct ttttctgcct tcacaaaatt ttcaactgct gcttttgctt
     9901 catcaaaagt ttgtggacgt tctgcatcat aatcactcac tctcacaaac aatgtctgtg
     9961 gatttgcatc attcccaaca tctaaagcat cagaattttg tttattttgg cgtagctctc
    10021 cttcaaacaa aacttttaat actttctcat tttgtaaagc agtaggtacg ttttcacggg
    10081 taaacagatc cgttttttgc agttccacat taccggcttt cgcaacttct tcaagtgaag
    10141 aattattctc aaaggcttta tttgccattt cgcgcgcaac tgtaaaatac tcagttaaca
    10201 ctaattcttg gcgaatagtt tgggcaattt tatctttcac ttgtgctaac tcaatcactt
    10261 gctcaggttt acgctccaat actttgatga tatggtaagc attatccact ttcaccgctg
    10321 cactcatctg gccggcagtt aaatttgcta cagcatcttc aaaagctttc gggaaagtgc
    10381 cagcttttgc ccaacctaga tcaccaccat tatttgctga taaagtatcg ctagatttgt
    10441 tttttgccaa agtagcaaaa tcttccccat ttttgagcgc ttgctcaata gcctgtgctt
    10501 ccgcttcact agcgacttga atatgagcta aatgggcttc acctttagtt acataatcgg
    10561 ctttattgcg atcatagtaa gtttgaattt gctcattggt aatctcaact ttgctttcca
    10621 catcttttgg agaaagagta acgtactcaa ccgctaattt ttcaggtgct aataatttag
    10681 ttttatttgc ttcgtaatag gctcgcaatt cttgctcgga agcggtctga ttttccattt
    10741 cttttgcaac tgaaaattcg gctaaacgca cttcacgttg ttgcaacagt aattttgcta
    10801 acaatgcctg ctgtgccggt acattaaaat cactattgat aatcccctct tgtagctggg
    10861 attgcaacat accttcctgc acaatcgctg catagccatc ggcacttaaa ttattgtggc
    10921 gtaatgtttg ttggtataac tcgttactga atttaccatc ttgttgaaac atctggctgt
    10981 ttacaatttc agatttgatt tgatcagcac taatacctag ttttaactct tttgcatact
    11041 ggcgcagaag ctcttcatca attaatccat ttaataccga ttgattaaat tgagcagcat
    11101 actccgggct atccattaaa tcccaaaagc gactccccag ttcagcattt aatctatttt
    11161 gctcacgatt aagcgtgtta ttaaataatt gctgactaat ttcttcccca tttactttcg
    11221 ctacagaatt atcttgtgaa atcaatcctg tcccaattcc accgattaca aatgaaatag
    11281 agactaatgc aaaaataatt ttaaagaccg gcccatttgt ccgctcgtgc attttttcaa
    11341 tcatttaaaa attcctaaat tactcaaaaa ttgtcgctga ttatactctt tctcaaatga
    11401 gaaataaact tgcaaatttt tattgaaatt taaccgcttt ctctttaaaa ttcacagtcc
    11461 ctgtcgtagc tgagttagag atcggctatt gatgtattat caacccttaa caaaaggaaa
    11521 ttaaaatgtc tctaagcgta gaagcaaaag caaaaatcgt tgctgaattt ggtcgtgatg
    11581 caaaagacac tggttcttct gaagttcaaa ttgcattatt aactgcacaa atcaaccact
    11641 tacaagcaca ctttgctgag cataaaaaag atcaccacgg tcgtcgtggt ttattacgta
    11701 tggtttcacg ccgtcgtaaa ttattagact acttaaaacg tactgatctt gctaaatatt
    11761 cggaaactat cgcacgttta ggtttacgtc gctaatttca aatattagat atgagagccg
    11821 agctgaaaag ttcggctttt ttataaattt tattcgagga aaaaatatgg cttttgcgtc
    11881 attatttaca ttattagacg atattgctgc cgtgcttgat gatgtatcga tcatgactaa
    11941 agtggctgct aagaaaacag caggcgtaat cggtgatgat cttgccttaa atgctaatca
    12001 agtcagtgga aagaatgtgg tgccagaacg agaactaccg attgtttggg ctgtagcgaa
    12061 aggctcatta atcaataaag tgatcttgat tccactagcg ttgctgctat cggtcttttt
    12121 accgcaggct attatcccgc tattaatgat aggcggtgca tatttatgtt ttgaaggcgt
    12181 agagaaatta ctccatcgtc tattacataa aaacaatcaa gctcattctg atgaaaccga
    12241 aaccctttct gaaaaagata aaattaaagg tgcaattcgc accgatttta ttctatcagc
    12301 agagattatc attattgcgt taggtgtttt gcaagactct tcaaccctaa cccaaatttt
    12361 atctctctcc gctatcgggg tcggaattac cctgtttgtt tatggcttag ttggattaat
    12421 tgtaaaacta gatgatatcg gtttatggtt aatgcagaaa aaaagcacat tctcacaagc
    12481 ggttggaaaa ggcttattat ttgcaatgcc gtggtttatg aaaggacttt ctgtgattgg
    12541 tactattgcg atgttcttag tcggtggtgg tatttttacc cataatttac caatgattca
    12601 tcacttcgta gaacaattcg gtatctctcc ccatttttct actctggttg attttgcagt
    12661 gggtattgtg gttggtatta ttgcatgctt aattatttta cctttgatga aattattttc
    12721 taaaaaggcg cactaatccc caaaagcgta gctttggtta aagctagctt
//
LOCUS       DANLRK010000072        12659 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.072, whole genome shotgun sequence.
ACCESSION   DANLRK010000072 DANLRK010000000
VERSION     DANLRK010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 12659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12659
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.072"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            <1..926
                     /locus_tag="PXA33_001409"
     CDS             <1..926
                     /locus_tag="PXA33_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HDL5120733.1"
                     /translation="QLLTAKPINKEVLNQVLKTVFDNEHLLGIDLQVYGAEYWNVSID
                     NKPSLDWVSAEIAIENEKLGILSWKPSLVCPGERLIQNVSEMIGRSLYVVQMQKQQQQ
                     LVLMEERAIIARELHDSLAQSLTFFKIQVSLLKLAKTDEKRNEILTVFEKALNDAYSQ
                     LRELLTTFRLTIQEANLQQALEKILESLRPKTSAQIRLSCKLPSHMFNAQQQVHVLQI
                     VREAVINAIKHSQATEINVIAETNDDGEHCLTIQDNGVGLAAHTQPEGHYGLTIMQER
                     AAELKAEFSIENVESGGVKVQVLLPSMISKR"
     gene            977..1495
                     /locus_tag="PXA33_001410"
     CDS             977..1495
                     /locus_tag="PXA33_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDL5120734.1"
                     /translation="MQFRKAVEADFETILAIYNQAIPTHQITADLELATTQNRRKWFD
                     FHLETAQYPIWVVEDEKGIAGWFSFSPFYERPAFLHTSEISIYLDRYSKGKGYGSKII
                     EFMQAEMLNHNIHTLMAYVFELNTVSQNLMRKHGFEQWGRFPNIANMGKDEKGEDKWR
                     TLLMMSYQKGIE"
     gene            1538..2137
                     /gene="rdgB"
                     /locus_tag="PXA33_001411"
     CDS             1538..2137
                     /gene="rdgB"
                     /locus_tag="PXA33_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HDL5120735.1"
                     /translation="MEKTKIVLATSNADKVKEMADVLSQFGFEVVAQSEFGIESPEET
                     GLTFVENALIKARFAAKMTGLPAIADDSGLSVMALGGEPGLYSARYAGEQATDADNRQ
                     KLLAKMQGQENRLAKFVSCIVFLKHETDPTPYIASGECFGEILTEERGENGFGYDSLF
                     FYPPKACTFAELETKEKKAISHRAIALEQLKQQLAGENK"
     gene            2134..2454
                     /locus_tag="PXA33_001412"
     CDS             2134..2454
                     /locus_tag="PXA33_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HDL5120736.1"
                     /translation="MIITTTPTIEGKQIVEYKGLVFGEVVSGANFIRDFLAGITDIIG
                     GRSGAYESKLNGARKEALAELAKEAQKVGANAVVGVSMEYQSMGGDKGMFIVVATGTA
                     VVIR"
     gene            2458..2910
                     /locus_tag="PXA33_001413"
     CDS             2458..2910
                     /locus_tag="PXA33_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HDL5120737.1"
                     /translation="MQFEQHFEANFWQNKSLLEMNDAEWEALCDGCGKCCYRKYIQGR
                     GRRERLYYTSVACNLLDVNTGRCMNYSNRFKIESDCTKLTKKNLPDFGWLPKTCAYRL
                     LYEGKPLFDWHPLISKDPDSVKNAGMLIANGIHEKDVIDWFEFVIDEV"
     gene            complement(2951..3532)
                     /gene="lpcA"
                     /locus_tag="PXA33_001414"
     CDS             complement(2951..3532)
                     /gene="lpcA"
                     /locus_tag="PXA33_001414"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HDL5120738.1"
                     /translation="MYLEQIKAELQEAADVLDKFMSDEKNIKLIQDAAMLIANSFKQG
                     GKVISCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHLSCVSNDFGYEYVFS
                     RYLEAVGQKGDVLFGLSTSGNSKNVLNAIKVAKEKGMKVIAMTGKDGGQMAGLADVEI
                     RVPHFRYADRIQEIHIKVIHILMMLIEFEMEKA"
     gene            3841..4800
                     /locus_tag="PXA33_001415"
     CDS             3841..4800
                     /locus_tag="PXA33_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL5120739.1"
                     /translation="MVVNPESFEQIIGQSVKIKEVVEQAKKFANLDAPLLIQGETGTG
                     KDLFAKSCHHFGSRRMQKFIAVNCAGLPAEEAESEMFGHRGNGVESVGFFEYAHGGTV
                     LLDSISELSLEMQAKLLRFLNDGSFRRVGEDQEIQVNVRVICTSLQPLSLLVEKGKVR
                     EDLFHRLNVLTLNLPPLRDRKEDIPLLADYFIAQISNQLGVSKLEYDNVFLQALQNYH
                     WRGNLRELYNALYRACTLSHSYRLAVKDLHLPRDMEAMIEVREEATLEEMMSQYEAQL
                     LHQFYAEYPSTRKLAQRLGLSHTAVANKLRQYGLTKNGNSKDE"
     gene            4793..6544
                     /locus_tag="PXA33_001416"
     CDS             4793..6544
                     /locus_tag="PXA33_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL5120740.1"
                     /translation="MNKLAVIFSVCFANLGLNSTAYAAPSIPQELLENSLVYCTTSSG
                     FSFNPQKSDVSSNMNVVTEQIYDKLVEFNPKKNRLEPALAESYSISDDGLVITLRLRK
                     NVAFHTTPWFTPSRKLNAEDVVFSLKRMIGKESDLPEVKSEPESVLHHQRQSYIYQKT
                     AGQIYFPYFESVDLANKIASIHSPSNDIVKIELVRPDSALLAHLASQYAVILSKEYAL
                     QLNADDNLVQLDLLPVGTGVYQLESYENNDYVRLKPNMNYWGKKAYIQNMVVDFSTEA
                     TGRMAKFLNNECDVVAFPEPSQLSVLNNRQLISSKGANLVYLAFNMQRGIGQDLALRQ
                     RIARGINRKRIAEKLFYGTGEVADNLLPTALWSKKNNGSYPYQPHQNSGEQAVEMHDS
                     IINRSLDSKTETEEVNQKQRLKLWVIDEKRVYNQHPLKMAEMIRADLATQNLEVEIRA
                     VSRAYLVQQLANKSADYDLILGGWLANNLDPNSFLEALLSCKAAETVTNLSNWCNEDF
                     DFWLQTARLSDDPASANLLYEFAQKLLEEQLPILPLMNANRVLVIKDGVKQADISPFG
                     QVKLSELRLGLGHKKGN"
     gene            6547..7512
                     /locus_tag="PXA33_001417"
     CDS             6547..7512
                     /locus_tag="PXA33_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDL5120741.1"
                     /translation="MLIVFFRKIFLTLITLIFLSLISFNILLRDPLNHLAELGWFEAY
                     CYYVKSLLNGDFGISFSNGEPLTAQILQVFPATITLCLSALIVSLIIGLPLGFFAATQ
                     QKNMVGRLLNTLGSLSLAVPVFWLALVLMYYASLNQWEVSAIGEIHSLYPKQMVTGFL
                     LLDIWLSDVPYKLKMMQSALHHLALPTLVLAVPATLEVMHITQARAGYVMKQNYIKIA
                     KTRGWTPFRVWRTHIICNTLPALLPMIARTFILIFAFGMLIENIFSWGGIGRWLINAL
                     AIQDYNAISAGVVAIGVFVLLVDMVTGFVRVMLDPSDKKDWYGSY"
     gene            7499..8392
                     /locus_tag="PXA33_001418"
     CDS             7499..8392
                     /locus_tag="PXA33_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDL5120742.1"
                     /translation="MAVIKEPEQFRETNAFKAFWFHFRQDKLALGSFYLFLLLLALIF
                     VGSLLAPYNYNEQFVGLELMPPSWDDKGQISHFLGTDDLGRDILSRIMYGFYYTLGSA
                     LMITLLIAIIGGMIGIYAGLKKGRSFWLISHLFDTFLFMPILLIAIIIATLMEASLVN
                     AMLAIFLAMLPHFIHKIYQATEKQLKKEYIITLKLDGASCWYLIREVIIPNLTGVAIK
                     ALSHIFVIAILDISALSFIMLGAKSPTPEWGAMIRESMELIYIAPWTVILPGLAIIFS
                     IFTVTILGTRLSVIFEKYRKE"
     gene            8463..9515
                     /locus_tag="PXA33_001419"
     CDS             8463..9515
                     /locus_tag="PXA33_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL5120743.1"
                     /translation="MALLDIRHLSIEIDMPKGRVKMVDNINLTVDEGEICGLVGESGS
                     GKSLIAKVICGMIKDEWIVSADRFRFDDIELLKLSPAERRKLVGDHISMVFQDPLTSL
                     DPSQQIGKQLMQTIQFRGKWWQRFGWKKKKAIELLHRVGIRDHQDIMKSYPSDITEGE
                     AQKVMIAMAVANQPRLLVADEPTNIMESTTQLQVYRLLSSMNQNLGTSILLVSNDMRS
                     IQNWVDSYNVLYCGQTVEIADKESILESSYHPYTRVLLNSSPDFSQPLAFKSSLNTLR
                     GSVPMLQEMPIGCRLGPRCPFAQKKCINKPPLVKVKQHEFACHFPINFREQNLLKNKT
                     ENTPFVVAEIAEKEAF"
     gene            complement(9524..9754)
                     /locus_tag="PXA33_001420"
     CDS             complement(9524..9754)
                     /locus_tag="PXA33_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HDL5120744.1"
                     /translation="MKLTACKLVFLLYLMFNLTACGTVLSLVEQDYRVYAGVIKDFQV
                     MQEGGIFAVLAVIDLPLSFVLDTLMLPVTLSQ"
     gene            9884..10966
                     /gene="leuB"
                     /locus_tag="PXA33_001421"
     CDS             9884..10966
                     /gene="leuB"
                     /locus_tag="PXA33_001421"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HDL5120745.1"
                     /translation="MQTYNIAVLAGDGIGPEVMAEAIKVLEATQQKFGFKLNFNQYNI
                     GGAAIDAQGKALPENTLKGCEEADAILFGSVGGPKWTHLLPDEQPERGSLLPLRKHFS
                     LFCNLRPAALYQGLEKFCPLRADIAAKGFDMVVVRELTGGIYFGQPKGRDGEGAQTRA
                     FDTEVYYKYEIERITRVAFESAMKRKKHVTSVDKANVLQSSILWRETVTEVAKEFPEV
                     TLEHMYIDNATMQLIKAPESFDILLCSNIFGDIISDEAAMITGSMGMLPSASLNEQGF
                     GLYEPAGGSAPDIAGKNIANPIAQILSAAMMLRYSFNLGDAADAIETAIQKALADGYR
                     TADLADDSKPLSTSEMGDIIAKNILA"
     gene            complement(11053..11448)
                     /gene="msrB"
                     /locus_tag="PXA33_001422"
     CDS             complement(11053..11448)
                     /gene="msrB"
                     /locus_tag="PXA33_001422"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HDL5120746.1"
                     /translation="MIKPIHALTEQQVDILINHGTERPFTGKFLNEHRVGIYRCARCH
                     NDLFHSDTKFDAGCGWPSFYQTISESSLRYLDDYSLGRHRIEIRCGNCDSHMGHVFPD
                     GPPPTGLRFCLNSAALNFKWEETGEEIDG"
     gene            11673..>12659
                     /gene="gap"
                     /locus_tag="PXA33_001423"
     CDS             11673..>12659
                     /gene="gap"
                     /locus_tag="PXA33_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDL5120747.1"
                     /translation="MAIKIGINGFGRIGRIVFRAAQHRDDIEVVGINDLIDVDYMAYM
                     LKYDSTHGRFDGTVEVKDGQLVVNGKAIRVTAERDPANLKWNEIGVDIAVEATGLFLD
                     DETARKHITAGAKKVVLTGPSKDATPMFVNGVNFDTYAGQDIVSNASCTTNCLAPLAK
                     VIHEKFGIKEGLMTTVHATTATQKTVDGPSAKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPALNGKLTGMAFRVPTTNVSVVDLTVNLEKPATYAEICAEIKRASENEMKGVLGYTE
                     DAVVSTDFNGATETSVFDAAAGIALTDTFVKLVSWYDNEVGYSNKVLDLVAHV"
BASE COUNT         3928 a         2219 c         2730 g         3782 t
ORIGIN      
        1 ctcaattatt aactgctaag ccgattaata aagaagtgtt aaatcaagta cttaaaacgg
       61 tatttgataa tgaacatttg ctagggattg atttacaagt atatggggct gaatattgga
      121 atgtatcaat agacaataaa ccaagtttag attgggtttc tgctgaaatt gcaatagaaa
      181 atgaaaaatt gggtatctta agctggaaac cctcactggt ttgtcctggt gaacgattga
      241 tccaaaatgt gtcggaaatg attggtcgta gcctttatgt agtgcagatg caaaagcaac
      301 agcaacaatt ggtactcatg gaggagcgtg caattattgc tcgtgagtta cacgattcct
      361 tagcccaatc tcttacgttt ttcaaaattc aggttagcct gcttaagctt gctaaaacag
      421 atgaaaagcg taatgaaatt ttaactgtat ttgaaaaagc gttaaatgat gcttacagcc
      481 aattaaggga gctgttgacg acgtttaggc taacgattca agaagcgaat ttacaacaag
      541 cgttagaaaa aattttagaa agcttaagac cgaaaacctc agcacagatt cgcttaagtt
      601 gcaaattacc ttcgcatatg tttaatgctc agcagcaagt tcacgtattg caaattgtac
      661 gagaagctgt gattaatgct attaagcatt cgcaagccac tgaaattaat gtgattgctg
      721 aaacaaatga tgatggtgaa cattgtttga ctattcaaga taatggcgta gggcttgctg
      781 cccatacgca accagaaggg cattatggac tgacgattat gcaagaacgg gcggctgaac
      841 tcaaagccga attttcgatt gaaaatgtgg aaagtggcgg agtaaaagta caggttttat
      901 tgccgagtat gattagcaag cggtaataaa atattaattt tttgcgtatt attggcaacg
      961 aataaaagga taacaaatgc aattccgaaa agcagtagaa gcggattttg agacaatatt
     1021 ggcaatttat aatcaagcaa ttcccactca ccaaattacc gccgatttag aactggcaac
     1081 tacgcaaaat cggcggaagt ggttcgattt tcaccttgaa acagcccaat atcctatttg
     1141 ggtggtcgaa gatgaaaaag gcattgctgg ctggtttagt ttttcaccat tttatgaaag
     1201 accggcattt ttacatactt cggaaatcag tatctattta gaccgctatt ccaaaggaaa
     1261 aggctatggc tcaaaaatta ttgagtttat gcaagctgaa atgcttaatc acaatattca
     1321 tacgttgatg gcgtatgtgt ttgaattaaa tacagtgagc caaaatctaa tgagaaaaca
     1381 cggctttgaa caatggggaa gatttccgaa tattgctaat atgggaaaag atgagaaagg
     1441 tgaggataaa tggcgaacgt tgctgatgat gtcttaccaa aaaggtatcg aataacaagc
     1501 ggtgattttt tagaatattt ttgcaaataa ggacaatatg gaaaagacaa aaattgtact
     1561 tgcaacaagt aatgcagata aagttaaaga aatggcagat gtactctcac aatttggttt
     1621 tgaggtggtg gctcaaagtg aatttgggat tgaatcaccg gaagaaaccg gtttgacttt
     1681 tgttgaaaat gcgctgatta aagcacgatt tgctgctaaa atgacaggtt taccggcgat
     1741 tgccgatgat tcaggtttat cggttatggc acttggtggt gaaccggggc tatattctgc
     1801 acgttatgcc ggagagcagg caaccgatgc cgataatcgc caaaaattac tagccaaaat
     1861 gcaagggcaa gagaatcgtc tggcgaaatt tgtcagttgt atcgtgtttt taaaacacga
     1921 aactgaccca acaccttata ttgcgtcggg tgaatgtttc ggtgaaattt tgacggaaga
     1981 aagaggtgaa aatggtttcg gctacgattc cctgtttttc tacccaccga aagcttgcac
     2041 ttttgccgaa ttagaaacca aagagaaaaa agcgatttca caccgtgcaa tcgcactaga
     2101 gcaattaaaa cagcaattag caggagagaa taaatgatta ttacaactac acctacgata
     2161 gagggtaaac aaattgtaga atacaaaggg ttagtatttg gcgaagtagt ttctggggct
     2221 aattttatcc gtgatttctt agccggtatc accgatatta ttggcggacg ttcaggtgct
     2281 tatgaaagca aattaaacgg tgcaagaaaa gaggcattag ctgaattagc taaggaggca
     2341 caaaaagtgg gggcaaatgc ggtggttggc gtttctatgg agtatcaatc aatgggcggc
     2401 gataaaggaa tgttcatcgt ggtagcgact ggtactgcgg ttgtaatacg ttaatttatg
     2461 cagtttgaac aacactttga agcaaatttc tggcagaaca aatcgctact tgaaatgaat
     2521 gatgctgaat gggaagcatt atgtgacggt tgtggtaagt gctgctaccg gaaatatatt
     2581 cagggaagag ggcggcgtga acggctttac tatacctcgg ttgcctgtaa tttattagat
     2641 gtgaatacag gccgttgtat gaattactcg aaccgtttta aaattgagtc ggattgtaca
     2701 aaattaacta aaaagaactt acctgatttt ggctggttgc caaaaacctg tgcttatcgt
     2761 ttactttatg aaggaaagcc tctttttgat tggcaccctt taatctctaa agatcctgat
     2821 tctgtaaaaa atgccggaat gctgattgct aacggtattc acgaaaaaga tgtgattgat
     2881 tggtttgagt ttgttattga tgaagtatga gaaaaaggcg tttggtgcaa acgccttttt
     2941 gttatagaga ttaggctttt tccatctcaa attcaattaa catcatcaaa atatgaatca
     3001 ctttaatgtg aatttcttgg atgcggtctg cgtaacggaa atgaggtact cggatttcca
     3061 catcggctaa tcctgccatt tgcccgccat ctttgccggt cattgcaatc actttcatgc
     3121 ctttttcttt ggcaactttg atcgcattta acacattttt agagttacca gaggttgata
     3181 gaccaaagag tacatcgcct ttttgcccaa cggcctctag ataacgagag aagacatatt
     3241 cataaccgaa gtcattactt acacagctta aatggctgac atcggaaata gcgattgccg
     3301 ggtatccggg acggttttca cggtaacgac ctgttaattc ttcggcaaag tgcatcgcat
     3361 cacagtgcga accgccatta ccgcaagaaa tcaccttacc accttgcttg aaactatttg
     3421 caatcagcat ggcggcatct tgaattaatt taatattttt ttcatcagac ataaatttgt
     3481 ctaatacatc tgcagcttct tgaagctcag ctttaatttg ttctaaatac atcatttgct
     3541 cctttgtttg caaaacgctt acctttaaac atcggcttta ccaatggggg tagcgagtaa
     3601 aaaattcgtt ttttatgacc gcttgaggca taaaaagtgc aataagttta gaagatctat
     3661 gctgtattgt aaacctaata acttaaataa tctgaatagg gttgtaaaca aaagtttcca
     3721 aaagagaaaa atagcggtat gatcgcctca caatccctct cgcattgtta actctagtag
     3781 ctaatatcag gctttaagcg tattaaacaa tgcacaaatt tgctatttat aggagactat
     3841 atggtcgtaa atccagaatc ttttgaacag ataattggac aatctgtaaa gataaaagag
     3901 gttgttgaac aagcaaaaaa atttgccaac cttgatgcac cattattgat tcaaggtgag
     3961 acgggaacag gaaaagattt atttgccaaa tcttgtcacc attttggatc tcgtcgaatg
     4021 caaaaattta ttgccgtgaa ctgtgccggt ttaccggctg aagaagcaga atcggaaatg
     4081 ttcggacatc gtggcaatgg cgttgagtcc gtcggtttct ttgaatatgc tcacggtggt
     4141 acagtattat tagactctat ttctgagtta tcgctggaaa tgcaagccaa attattgcgc
     4201 tttttaaatg atggctcttt tcgtcgagtg ggcgaagatc aagagattca agtcaatgtt
     4261 agggtgatct gtacttcatt acagcctttg tcattattag ttgaaaaagg caaagtaaga
     4321 gaagatctgt ttcatcgttt aaatgttctc actttaaatt taccaccttt gcgagatcga
     4381 aaagaggata ttccattatt agctgattac tttatcgccc aaattagcaa tcagttgggg
     4441 gtgagcaaat tagagtatga taatgttttt ttgcaagcat tgcagaatta tcactggcga
     4501 ggcaacttaa gagagcttta taacgcactt tatcgagctt gtacactttc gcatagctac
     4561 cgtttagcgg taaaagatct tcatttaccc agagatatgg aggcgatgat tgaagtacgt
     4621 gaggaggcga cacttgagga aatgatgagc cagtatgaag cccaattatt gcaccaattt
     4681 tatgcagaat atccgagtac ccgaaaatta gcacaacggc tcggactttc acatactgcg
     4741 gtggcaaaca aattgcgtca atatgggtta acaaaaaatg gaaacagtaa agatgaataa
     4801 actagcggtc attttttcag tatgttttgc aaatttaggg ctaaattcga ccgcttacgc
     4861 agcgccaagt attcctcaag agctacttga aaatagcctt gtgtattgca ctacttcatc
     4921 gggattctct tttaacccac aaaaatccga tgtgagttcg aatatgaatg tggtaacaga
     4981 gcagatctac gataaattag ttgagtttaa tccgaaaaaa aatcgactag agcctgcttt
     5041 agctgaaagt tattcaatca gcgacgatgg tttagtgatt acattacgtt taagaaaaaa
     5101 tgtcgctttt catacaactc catggtttac tccgagtcgt aaattaaacg cagaagatgt
     5161 ggtgttttcg ctaaaacgaa tgattggtaa agaaagtgat ttacctgaag tgaaaagtga
     5221 gcctgaaagt gtattacacc accagcgaca aagctatatt taccaaaaaa cagcggggca
     5281 gatttatttt ccttattttg aaagtgttga tttagcgaat aaaattgcca gcattcattc
     5341 tccgtcaaat gacattgtaa aaattgaatt agtccgtcct gattcagcat tgttggcgca
     5401 tttagccagc caatatgcag ttattctgtc taaagaatat gctttacagc tcaatgcaga
     5461 tgataactta gttcaactgg atttattacc ggtaggaaca ggcgtatatc aacttgaaag
     5521 ttatgaaaat aatgattatg tgcgtttaaa accaaatatg aattactggg gcaaaaaagc
     5581 atatattcaa aatatggtcg tagatttttc cacagaagcg accggcagga tggcaaaatt
     5641 tttaaataat gaatgtgatg tggttgcttt tccggaacct agccaattat cagtgttaaa
     5701 taatcggcag cttattagta gtaaaggtgc taatttggtt tatttagcat tcaatatgca
     5761 acggggtatt gggcaggatt tggcattacg ccaacgtatt gcacgcggca taaatcgcaa
     5821 acgtattgca gagaaattat tttatggtac aggtgaagta gcggataacc tattaccaac
     5881 agctttgtgg tcgaaaaaaa ataatggaag ctacccttat caaccacacc aaaattcagg
     5941 tgagcaagcg gttgaaatgc atgacagtat tataaatcgg tctttagata gcaaaacaga
     6001 gaccgaagaa gtaaaccaaa aacagcgctt aaaactgtgg gtaattgatg aaaaacgagt
     6061 ttataatcaa cacccattaa aaatggcaga aatgatacgt gctgatttag ctacgcaaaa
     6121 tttagaggtc gaaattcgcg cagtaagtcg agcttatttg gtgcaacagc ttgctaataa
     6181 aagtgcggat tatgatttga ttttaggtgg ttggttagca aataatcttg atcctaacag
     6241 ttttttggag gccttactca gttgtaaagc tgcggaaacg gtgactaatt tatcaaattg
     6301 gtgcaatgaa gattttgatt tttggttaca aacagcccgt ctaagtgatg atccggcatc
     6361 agctaattta ttgtatgaat ttgcacaaaa attattagaa gagcaattgc ctattctccc
     6421 tttaatgaat gctaatcgtg tattagtcat aaaagatgga gtgaaacagg cagatatttc
     6481 ccctttcggc caggtaaaat tatcagagtt acgtttgggc ttggggcata aaaaaggaaa
     6541 ctaattatgc tgattgtctt ttttcgaaaa atttttctaa ccttgattac gttgattttt
     6601 ttgtcactta tcagttttaa tattctgtta cgagatccat taaaccactt agccgagtta
     6661 ggctggtttg aggcttattg ttattatgta aaatctctgc taaatggtga ttttggtatt
     6721 agtttcagta atggagaacc gctcacagcg caaattttac aggtgttccc ggctacaatt
     6781 acgctttgtt tatcggctct cattgtttct ttaattattg gtttgcctct tggttttttt
     6841 gcggctactc agcaaaaaaa tatggttggg cgattattaa atacactagg ttcattgagt
     6901 ttagccgtgc cggtattttg gctggcatta gtgctaatgt attacgcttc tctcaatcag
     6961 tgggaagtgt cagcaattgg agaaatccat tcgctttatc cgaaacagat ggtaaccggc
     7021 tttttgctat tagatatttg gttatctgat gtaccttata aattaaaaat gatgcagagt
     7081 gctttacatc acttagcttt gccgacatta gtcttggctg tgccagctac attagaagtt
     7141 atgcatatta ctcaagctag agcaggttat gtaatgaaac aaaattacat taaaatagcg
     7201 aaaacgcgtg gctggacacc gtttagagtt tggagaacgc atattatttg taatacttta
     7261 cctgcattat tacctatgat tgcccgtacc tttattttaa tttttgcctt cggtatgtta
     7321 attgagaaca tatttagctg gggaggaatt ggacgttggc tgataaatgc ccttgctatt
     7381 caagactata atgcgatttc agcgggagta gttgcaatcg gggtgtttgt gttattagta
     7441 gatatggtta caggctttgt gcgggtgatg ttagacccat cagataaaaa ggattggtat
     7501 ggcagttatt aaagaacccg agcaatttag agaaaccaac gcatttaagg cattttggtt
     7561 tcatttccga caagataaac ttgcacttgg cagtttttat ctttttttgc tattactggc
     7621 tctcattttt gttggtagtt tgcttgctcc ttacaattat aatgaacaat ttgttggttt
     7681 agaattaatg cctccctctt gggacgacaa aggtcaaatc agccattttt taggcactga
     7741 cgatttagga cgggatattt taagccgtat tatgtatggt ttttactaca cgcttggttc
     7801 cgcactgatg attaccttat taattgcgat tatcggcggt atgatcggga tttatgccgg
     7861 gctgaaaaaa ggacgttcat tttggttgat tagccatttg ttcgatacct ttttatttat
     7921 gccgatttta ttgattgcga tcatcattgc aaccttaatg gaagcgagtt tggtcaatgc
     7981 aatgttggcg attttcttag cgatgttgcc gcattttatc cataaaattt atcaagcaac
     8041 cgaaaaacaa ctcaaaaaag agtacatcat taccttaaag ctagacggtg caagctgctg
     8101 gtatttaatt cgagaagtga ttattcctaa tcttaccggc gtggcaatta aagcactttc
     8161 acatattttt gtgatcgcca tattagatat tagtgcgtta agttttatta tgctgggggc
     8221 taaaagcccg acccctgaat ggggagcgat gattcgtgaa tccatggaac tgatttatat
     8281 agctccttgg acggtgatat tacctggctt agcaattatt tttagtattt ttaccgttac
     8341 aatacttggt acacgcttaa gcgtgatttt tgaaaaatac cgtaaagaat agtatttaaa
     8401 taggatactg acacataaca agcggtcatt tttaagtaaa attttacaga ttaaggaata
     8461 gtatggcatt acttgatatt cgccatttaa gtattgaaat tgatatgcca aaaggcagag
     8521 taaaaatggt ggataacatc aacctgactg ttgatgaggg cgaaatttgt ggcttggtgg
     8581 gagaatctgg ttcgggaaaa agcctcatag ccaaagtgat ttgcggtatg attaaagatg
     8641 aatggattgt gagtgcagat cgctttcgct ttgacgatat tgaattactg aaactttcgc
     8701 cagcggaacg ccgaaaattg gtaggcgatc atatttcaat ggtattccaa gatccgctta
     8761 ctagccttga cccaagccag caaattggta aacaactgat gcaaaccatt caatttagag
     8821 ggaagtggtg gcaacgattt gggtggaaaa agaaaaaagc aatcgagcta ctacaccgtg
     8881 ttggtattcg tgatcatcaa gatattatga aaagttaccc aagcgatatt accgaaggcg
     8941 aagctcagaa agtgatgatt gcaatggcgg tggcaaatca gccccgatta ttggtggcag
     9001 atgagccgac taatattatg gaatcaacca cgcagttgca ggtttatcgc ttgctttcca
     9061 gtatgaatca gaatttggga acctcaatct tgctggtgag taatgacatg cgtagcattc
     9121 aaaattgggt tgattcttac aatgtgcttt attgtggaca aaccgttgaa attgcggata
     9181 aagagagcat tcttgaatcg tcttaccacc cctatactcg agtgttactc aatagttcgc
     9241 cggacttttc tcagccttta gcctttaaaa gtagcttaaa tacgctacga ggctcagtcc
     9301 cgatgttaca agaaatgccg attggttgtc gtttaggccc tcgttgccct tttgcacaaa
     9361 aaaaatgtat caacaaaccg ccattagtga aagtaaaaca gcacgagttt gcctgccatt
     9421 ttccgattaa ctttagagaa caaaatttgc ttaaaaataa aacggaaaat acgccgtttg
     9481 tggtggctga aatagcggaa aaagaggcgt tttaatgtgg ttattattgg ctaagtgtaa
     9541 cgggtaacat taaagtatct aatacgaaac tcaagggtaa atcgatcacc gcaagcaccg
     9601 cgaatatacc gccttcttgc attacttgga aatcttttat tacgccggca taaacacggt
     9661 aatcctgttc aaccaagctt agtactgtgc cacaagcggt gaggttaaac atcaaataga
     9721 gtagaaaaac gagtttacaa gcggtcaatt tcatcaaaaa atctccaaat acgcttttat
     9781 tttacgctat ttttatcggg ttggcgtaaa attcgtttta aaaacagcta ttttcaggca
     9841 gaatgtagcc gaatcgacat taaatatact aagaggatct tatatgcaaa cttacaacat
     9901 tgcagtgctt gccggtgacg gcattgggcc ggaagtgatg gcagaagcta tcaaagtgct
     9961 tgaagcgact caacaaaaat ttggctttaa actaaatttt aatcaatata acattggtgg
    10021 agcagcgatt gatgctcaag gtaaagcgtt acccgaaaat accttaaaag gctgtgaaga
    10081 ggcagatgcg attttgtttg gctcggtagg tggcccgaaa tggacgcatt tactacctga
    10141 tgagcaacct gaacgaggtt cgctattacc actgcgtaaa catttctcgc tattctgtaa
    10201 tttacgccct gctgcattgt atcaagggct ggaaaaattc tgcccattac gtgccgatat
    10261 tgctgctaaa ggttttgata tggtggtggt gcgtgaatta accggcggga tttattttgg
    10321 acagccgaaa ggccgtgatg gtgaaggtgc gcaaacgaga gcgtttgaca ccgaagtata
    10381 ctacaaatat gaaatcgagc gtattacccg tgtggcgttt gaatctgcaa tgaaacgtaa
    10441 aaaacacgta acctctgtgg ataaagccaa tgtgttacaa agctcgattt tatggcgtga
    10501 aaccgtcacg gaagtagcta aagagttccc agaagtcacg cttgagcata tgtatattga
    10561 taatgcgaca atgcagctaa tcaaagcacc tgaatcgttc gatattttat tatgctcaaa
    10621 catcttcggc gatattattt ccgatgaggc tgcaatgatt acaggttcta tgggcatgtt
    10681 gccttcagcc agcctcaatg agcaaggttt cggcttatat gaaccggcag gcggttcagc
    10741 accggatatt gccggtaaaa atattgcgaa tccgattgcg caaattttat cagccgcaat
    10801 gatgttgcgt tatagcttta acttaggtga tgctgctgat gcgattgaaa cagcgatcca
    10861 aaaagccctt gctgatggct accgtaccgc tgatttagct gatgacagca aaccgctttc
    10921 tacctcagaa atgggcgata ttattgcgaa aaatattctc gcttaaggct agaagttaaa
    10981 tagcacaagc ggtcattatt cgccaaattt ttgcaaattt tccgctaata atgaccgctt
    11041 gtttattact cgttagccgt caatctcttc gcctgtttct tcccacttaa aattcaatgc
    11101 cgcagagttg aggcaaaaac gcaaccctgt tggtggtggg ccgtccggaa aaacgtgtcc
    11161 catatgcgaa tcacaattcc cacaacgaat ttcgatacga tgacgtccta aactgtaatc
    11221 atctaagtaa cgtagtgacg attctgaaat cgtttggtaa aaacttggcc agccacaacc
    11281 ggcatcaaat ttagtatctg agtgaaataa atcgttatgg catctggcac aacgataaat
    11341 tcctacccga tgttcattca aaaattttcc ggtaaacgga cgttccgtac cgtggttgat
    11401 taaaatatca acttgttgtt ctgtcagggc gtgaataggt ttaatcatca gcaattctcc
    11461 taagatagta gcgaagtcat tgtagctttt tttgacctga aaatcgttat aaatttcaaa
    11521 atttgacata gatcacaaaa aatttatctg ttatattgga agggttcttt tggcttgtca
    11581 ggaaagtgaa aagatgtgct ataattcaag catctttagt gtagtacata aagaacaaat
    11641 tttgtttaac tttaatatag gtagaaaaat ctatggcaat taaaattggt attaacggct
    11701 ttggccgtat cggtcgtatc gttttccgtg cagcacaaca ccgtgatgac atcgaagtgg
    11761 taggtatcaa cgacttaatc gatgttgatt atatggctta tatgctgaaa tatgattcaa
    11821 ctcacggtcg ttttgacggt actgttgaag ttaaagacgg tcaattagta gttaacggta
    11881 aagcgatccg cgtaacagct gagcgtgacc cggctaactt aaaatggaat gagatcggtg
    11941 ttgatatcgc agttgaagca acaggtttat tcttagatga tgaaactgct cgcaaacaca
    12001 tcaccgcagg tgcgaagaaa gttgttttaa ccggtccgtc taaagatgca acacctatgt
    12061 tcgtaaacgg cgtaaacttt gatacttacg caggtcaaga catcgtgtct aacgcttctt
    12121 gtacaactaa ctgtttagca ccattagcga aagttattca cgaaaaattc ggtatcaaag
    12181 aaggtttaat gacaactgtt cacgcaacta ctgcaacgca aaaaacagta gatggtccat
    12241 cagcgaaaga ctggcgtggt ggtcgtggcg cgtcacaaaa catcattcct tcatcaacag
    12301 gtgcagcgaa agcagtaggt aaggtattac ctgcattaaa cggtaaatta accggtatgg
    12361 ctttccgtgt tccaaccact aacgtttctg ttgttgattt aactgtaaac ttagaaaaac
    12421 cagcaactta tgcagaaatc tgtgctgaaa tcaaacgtgc ttcagaaaac gaaatgaaag
    12481 gcgtattagg ctacacagaa gatgcagttg tttcaacaga cttcaatggt gcaactgaaa
    12541 cttcagtatt tgatgcagca gcaggtatcg cattaactga tactttcgtt aaattagtat
    12601 cttggtacga taacgaagtt ggctactcaa acaaagtatt agacttagtt gctcacgta
//
LOCUS       DANLRK010000073        12463 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.073, whole genome shotgun sequence.
ACCESSION   DANLRK010000073 DANLRK010000000
VERSION     DANLRK010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 12463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12463
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.073"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            <1..723
                     /gene="fkpA"
                     /locus_tag="PXA33_001424"
     CDS             <1..723
                     /gene="fkpA"
                     /locus_tag="PXA33_001424"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDL5120748.1"
                     /translation="LKTKLSLFTLIVGTLFSTNVMAEEKADPKFVDDSSYAVGVLMGK
                     NINGVIESQKDIFTYNQDKILAGVQDTLKKTGKLTDEDLQKQLKSLDAYLSAKEAKIN
                     EEKTKATVAAGDKFRADYKKQSGVKETASGLLYKIEKAGEGNSPKAEDTVKVHYKGTL
                     TDGTVFDSSYDRGEPIEFQLNQLIPGWIEAIPMLKKGGKMEIVVPPALAYGDRAAGKI
                     PANSTLKFEIELLDFKAADAKK"
     gene            820..1476
                     /locus_tag="PXA33_001425"
     CDS             820..1476
                     /locus_tag="PXA33_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL5120749.1"
                     /translation="MTKKFTPFSDEDHAILASYFPMVDGIAALIGEHCEIVLHSLEFL
                     EHSAIYIANGHNTNRKIGSPITDRALKSLHHMATDSVSKPYFTKAKGGVLMKSITIAI
                     RNGKQHVIGLLCININLEVPTSQFLNSFIAPVDTASEVTFASSVEDLVSQTIQTTIDD
                     VMADRQISNNNKNRQIVISLFEKGIFDIKDSINQVAEKLDISRHTVYLYIRQIKQDND
                     "
     gene            1476..1853
                     /gene="tusD"
                     /locus_tag="PXA33_001426"
     CDS             1476..1853
                     /gene="tusD"
                     /locus_tag="PXA33_001426"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HDL5120750.1"
                     /translation="MDYVLAIKSPVYGSQGAFLAYQVAEELLKANHTICQIFFFQEGV
                     SNANALVNPASDEVNLVEMWKKLAKLHRLPLHLCIAAAQRRGVVDEQISANATHNLAE
                     GFTLAGLGEFSQAVLKADRLLTF"
     gene            1855..2226
                     /gene="tusC"
                     /locus_tag="PXA33_001427"
     CDS             1855..2226
                     /gene="tusC"
                     /locus_tag="PXA33_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HDL5120751.1"
                     /translation="MKKYKLAFLFTQPPFGSAVSREGLDALLAASAFCDEQEIAILFI
                     NDGVFNLIADQQPEKLLQKDHISTFKLLELYDLTECFICQESVSLRGLDNADWLLRML
                     QFKTHAEMIALLNQAEKVMTF"
     gene            2236..2511
                     /gene="tusB"
                     /locus_tag="PXA33_001428"
     CDS             2236..2511
                     /gene="tusB"
                     /locus_tag="PXA33_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HDL5120752.1"
                     /translation="MLYTLSKANYDEETLNFFFSHLSENDAVVLWQDGVLQAVKSPQF
                     FAKLPRCYLLAQDVQARGLQEKLTAFQCISLSELVKLTAVYFPQVAL"
     gene            2627..3526
                     /locus_tag="PXA33_001429"
     CDS             2627..3526
                     /locus_tag="PXA33_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5120753.1"
                     /translation="MVMAEENNTWVDKKHETVSTTLDRWANNINSWLGDADPDKPGTA
                     SLRVMLDSRWDKHDKFTYKPRVRGKIKLPILKKHLNVVFGDDELDNELTDKNHAGKLY
                     KNVNPNKRYNSREARDSNSSIGLRWSDGIKALGIETDLDAGIRSGSDIFGRLRISKDW
                     KITDQFSTRLEQIYRYGSESKHYLRTNFENKYTETDTTFIMNHTNFEYRHDVDEERSW
                     GNSLYRQHNFKPLTHISYGISMGGRLDKELSKFNYWGPFVSYRQPIWRNWLFIQPEIS
                     FYNDKDKDRKHFIQTFVRLEAIF"
     gene            3876..7901
                     /gene="rpoB"
                     /locus_tag="PXA33_001430"
     CDS             3876..7901
                     /gene="rpoB"
                     /locus_tag="PXA33_001430"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDL5120754.1"
                     /translation="MAYSYSEKKRIRKSFGKRPQVLNVPYLLTIQLDSFEKFIEKDPE
                     GQQGLEAAFRSVFPIVSNNGATELQYVSYELGEPVFDVRECQIRGTTYAAPLRVKLRL
                     VSYDREAAAGTVKDIKEQNVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFARIDRRRKLPATIILRALGYTTE
                     EILNLFFEKIKFEIADNKLLMTLVPERLRGETASFDIAANGKVYVEMGRRITARHIRA
                     LEKDNVTQIEVPVEYIVGKVAAQDYVDLSSGEVICPANMEISMEMLANLSQAGYNQIE
                     VLFTNDLDHGPYISETLRVDPTYDRLSALVEIYRMMRPGEPPTKEAAEALFDNMFFSA
                     ERYDLSAVGRMKFNRSLEIPEGVGSGILSNDDIIGVMKKLIEIRNGRGEVDDIDHLGN
                     RRIRSVGEMAENQFRIGLVRVERAVRERLSLGDLDGVTPQDLINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEVTHKRRISALGPGGLTRERAGFEVRDVHPTHYGRLCPIETPEG
                     PNIGLINSLSVYARTNNYGFLETPFRKVVNGQVTEEIEYLSAIEESNNIIAQANSNLD
                     ENFRFTDTYVTCRQQGESGLFKPEEVQYMDISTQQVVSVAAALIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMEKPIALDSGVAIVAKRGGTVQYVDASRIVVKVNEDE
                     TIPGEAGIDIYNLIKYTRSNQNTCINQVPCVNLGEPVARGEILADGPSTDLGELALGQ
                     NIRVAFMPWNGYNFEDSMLVSERVVQEDRFTTIHIQELSCVARDTKLGAEEITADIPN
                     VGESALSKLDESGIVYIGAEVKGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGTSGTVIDVQVFTRDGVEKDKRAKEIEEMQLCEAKKDLAEELEILEAGLF
                     TRVRNLLIEGGVKESALDGVAREKWLEQTLEDEAKQNQLEQLAEQHEELRKEFERKLE
                     IKRNKIIQGDDLAPGVLKVVKVYLAVKRQIQPGDKMAGRHGNKGVISKINPVEDMPYD
                     ENGQPVEIVLNPLGVPSRMNIGQILETHLGLAAHGIGDQIDKMIKQQQEVAKLREYMQ
                     KAYDLGHGSQVVDLSTFSDEEVMRLAQNLRKGLPLATPVFDGAHESEIKGLLELGGLP
                     TSGQITLYDGRTGEKFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGKA
                     QFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGTHYMEPGIPE
                     SFNVITKEIRALAIDMELDEA"
     gene            8164..12447
                     /gene="rpoC"
                     /locus_tag="PXA33_001431"
     CDS             8164..12447
                     /gene="rpoC"
                     /locus_tag="PXA33_001431"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HDL5120755.1"
                     /translation="MKDLVKFLKAQTKSNEDFDVIKIGLASPDKIRSWSFGEVKKPET
                     INYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRER
                     MGHIELACPVAHIWFLKSLPSRIGLILDMPLRDIERVLYFESYVVTEPGMTDLEKNQL
                     LTEEQFMDAEERWGDEFEAKMGAEGIQALLRDLDLEHQCETLREELQETNSETKRKKI
                     TKRLKTLEAFLQSGNKPEWMVMTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINR
                     NNRLKRLLDLVAPDIIVRNEKRMLQESVDALLDNGRRGRAITGSNKRPLKSLADMIKG
                     KQGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFRPFIYSKLENRGIA
                     STIKAAKKMVEREDPIVWDILAEVIREHPILLNRAPTLHRLGIQAFEPLLIEGKAIQL
                     HPLVCAAFNADFDGDQMAVHVPLTLEAQLEARALMMSTNNVLSPASGDPIIVPSQDVV
                     LGLYYMTREKVNGKGEGMYFLDPREAEKAYRTGQAELHSRVKVRVTEYEKNEAGELEA
                     KTSLVDTTIGRSILWMIAPKGMPFSLFNQTLGKKAISKLINESYRRLGLKESVVLADQ
                     IMYTGFAYAARSGASVGIDDMVIPEQKYAIIEAAEAEVAEIQEQFNGGLVTAGERYNK
                     VIDIWAAANERVAKAMMENLSTEEVINREGNPEKQASFNSIFMMADSGARGSAAQIRQ
                     LAGMRGLMARPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLT
                     RRLVDVAQDLVIIEDDCGTHEGIVMTPLIEGGDVKEALRDRVLGRVVAEDVLKPGTDE
                     VLIPRNTLLDEKWCDVVDRESVDVIKVRSVVTCDTDFGVCAKCYGRDLARGHLINQGE
                     AVGVIAAQSIGEPGTQLTMRTFHIGGAASAAAKESSIQVKNAGTIKLTNAKFVTNKEG
                     KIVLTSRNTELTVIDTFGRTKENYKVPYGAVLSKHDGAEVAVGEVVANWDPHTMPVIS
                     EVSGRIQFSDIVDGLTVTRQTDELTGLSSIVVQDVGERATAGKDLRPALRLVDSNGKD
                     ILIPGTDVAAQYFLPGKAIVTLDDGAEIEVGEALARIPQESVGTKDITGGLPRVADLF
                     EARKPKEPAILAEISGIVSFGKETKGKRRLVITPAEGEAFEEMIPKWRQLNVFEGEMV
                     QRGDVISDGAETPHDILRLRGVHAVTDYIVNEVQEVYRLQGVKINDKHIEVIVRQMLR
                     KAVITNAYDSEFLEGEQVEVARVKIANRKRAEEGKPLVEFERELLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKQDELRGLKENVIVGRLIPAGTGFAYHQARAKKRAQLEA
                     GIAVVPEFEAPAAKANSFVTDADIEAEFEFVADDATQSLAALLNAGEEE"
BASE COUNT         3753 a         2418 c         2875 g         3417 t
ORIGIN      
        1 ttaaaaacca aattatcatt atttactctt attgttggca cactttttag tacaaatgtg
       61 atggcagaag aaaaagcaga tcctaaattt gtggatgatt cctcttatgc cgtgggtgta
      121 ttaatgggta aaaatattaa tggtgtgatt gagtcacaaa aggatatttt tacctataac
      181 caggataaaa tcttagccgg tgttcaggac accttaaaga aaaccggtaa attaaccgat
      241 gaagatttgc aaaagcagtt aaaatcgtta gatgcctatt tatctgcgaa agaagcaaag
      301 attaacgaag aaaaaaccaa agcaacggta gctgcaggcg ataaattccg tgctgattat
      361 aaaaaacagt ctggcgtaaa agaaacggct tcaggcctgt tatataaaat tgaaaaagca
      421 ggtgaaggca attcaccaaa agcggaagat actgtgaagg tgcattacaa aggcactttg
      481 actgatggca cggtatttga cagctcttac gaccgtggtg agccgattga atttcagtta
      541 aatcaattaa ttccgggttg gattgaagcg attcctatgt tgaaaaaagg cggtaagatg
      601 gaaattgtag tgccacctgc attagcttat ggtgatcgtg ccgcaggcaa aattccagca
      661 aattcaacct tgaaatttga aattgaatta ttagatttta aagcagcaga tgctaagaaa
      721 taattcgtta ttccttaatt attgaagata cgtaagcggt tagatttatt ataatatttg
      781 caaaaaatgt gataaaacta accgcttgta atttgtatta tgaccaaaaa atttacgcct
      841 ttttctgacg aagatcacgc cattcttgct tcttactttc cgatggtaga tgggattgcg
      901 gcactgattg gtgagcattg cgaaattgta ttgcattcgt tagagtttct ggaacattcg
      961 gcaatttata ttgctaacgg acacaatact aaccgtaaaa tcggctctcc gattactgac
     1021 cgtgcattga aatcgctgca tcatatggca acggatagcg tctccaaacc ttattttacc
     1081 aaggcaaaag gtggcgtttt aatgaagtct atcactattg cgattcgtaa tggtaaacag
     1141 cacgttatcg ggttactgtg cattaatatt aatttggaag tgcctacctc acagtttctc
     1201 aattctttta ttgcccctgt agataccgct tcagaagtta cttttgccag ttcagtagaa
     1261 gatttggttt cacaaacgat tcaaacgacc attgatgatg tgatggcaga tcgtcaaatt
     1321 tctaacaata ataaaaatcg tcaaattgtg atttcgttat ttgagaaagg gatttttgat
     1381 attaaagatt ccattaacca agtagcggag aagctggata tttcacgcca tactgtgtat
     1441 ctctatattc gtcaaattaa gcaggacaat gattaatgga ttatgtatta gcgataaaat
     1501 caccggttta tggttcacaa ggggcatttt tagcctacca agtggcggaa gaattattaa
     1561 aagccaatca tacaatttgc caaatattct tttttcaaga aggcgtgagc aatgctaatg
     1621 ccttagtgaa tccagcaagc gatgaagtga atttagtcga gatgtggaaa aaattggcta
     1681 aattgcaccg cttgcctctg catttgtgca ttgctgctgc ccagcgtaga ggcgtggtgg
     1741 atgagcaaat ctcagctaat gcaacacata atttagccga aggatttacc ctagccgggt
     1801 taggcgaatt tagccaagca gtattaaaag cggatcgatt actgactttt taagatgaag
     1861 aaatataaac ttgccttttt atttacccag ccaccttttg gttcggcagt tagccgtgag
     1921 gggctagatg ccttgcttgc agctagtgct ttttgtgacg agcaagagat agctatcctc
     1981 tttatcaatg atggcgtatt taatctcatt gccgatcaac agccggaaaa actcttacaa
     2041 aaagatcata tttccacctt caaattattg gagttgtatg acttaactga atgttttatt
     2101 tgccaagaaa gtgtaagttt acgagggctg gataatgcag attggttact gcgaatgctt
     2161 caatttaaaa ctcacgctga gatgatcgca ttgctcaatc aagctgaaaa agtaatgacg
     2221 ttttaaggaa tatttatgct ttataccctt tccaaagcga attatgatga agaaacctta
     2281 aacttctttt tctcacatct tagtgagaac gatgcggttg ttctgtggca agatggcgtg
     2341 ctacaagcgg tcaaaagtcc gcaatttttt gcaaaattgc cccgttgcta tttattggca
     2401 caagatgtgc aagctcgagg attgcaagag aagctcactg catttcagtg catttcatta
     2461 tccgaattag tcaaattaac agcggtttat tttccccagg ttgcactgta acctagagag
     2521 gatatttttt gtaataccaa ataatttaga ttatgatgtg attcctaaaa aggagaatat
     2581 tatgaaaaca aaatgtctta ttccgctact agcacttaca cctgttatgg tgatggcaga
     2641 agaaaataac acttgggtag acaagaaaca tgaaacagta agcactacct tagatcgttg
     2701 ggcaaacaat attaacagtt ggctaggtga tgcagaccca gataaaccgg gaacggcttc
     2761 tttacgggta atgttagata gccgttggga taaacacgat aaatttactt ataagccccg
     2821 tgttcgtggt aaaatcaaac tccctatctt gaaaaagcat ctaaatgtcg tatttggtga
     2881 tgacgaactg gataatgagc ttaccgataa aaatcatgct ggaaaattgt ataaaaacgt
     2941 caatccaaat aaacgctata actctcgtga agcgagagat tcgaactctt ctatcggttt
     3001 acgttggtct gatggcatta aggctttagg gattgagacc gatttagatg ctggtattcg
     3061 ttcgggaagc gatattttcg ggcgtttacg catcagtaaa gattggaaaa tcaccgacca
     3121 gttcagtacc cgcctagagc aaatttatcg ttacggttca gagagcaagc actatttacg
     3181 tactaacttt gaaaataaat ataccgaaac ggataccacc tttattatga accacaccaa
     3241 ttttgaatat cgtcatgatg tagatgaaga aagaagttgg gggaatagtt tatatcgtca
     3301 acataacttc aaaccactca cgcacatcag ctatggtatc agtatggggg ggcgtttaga
     3361 taaagagtta tcaaaattta actattgggg tccatttgtc tcctatcgtc aaccaatctg
     3421 gcgcaactgg ctctttatcc agcctgaaat cagtttttat aacgacaaag ataaagatcg
     3481 taagcacttt attcagacat ttgtacgttt agaggctatc ttctagctat taagcctcaa
     3541 gtagaatctt ggggcttgtt tttgcaagcg gtcaatttct tgccaaaatt tacaaaatcc
     3601 gctataattc ctaaccattt ttagcaaaaa ttggctgacg gcttgaccgg tcagcctttt
     3661 gtgcttgaac gcaagggctg attttgtcat tttaatcagc aaaatttgca aatttcctgc
     3721 ccaatttgac cgcttgcagt agcgatttct gactgcttga ttggcgattg gaaaatgaca
     3781 tctcgtacag tttttataat gccacttcca accgagtctt tcgtgtcgga ttgtgtaatc
     3841 tcaaccaaaa tatatttcag aggctatctt aaaaaatggc atattcatat tccgagaaaa
     3901 agcgtatccg taagagcttt ggcaaacgcc cacaagttct gaacgtacct tatctattaa
     3961 caattcagct tgattctttt gaaaaattta tcgaaaaaga tcctgaaggg cagcaaggtt
     4021 tagaagcggc attccgttct gttttcccga ttgtgagcaa caatggtgca acagaattac
     4081 aatacgtttc ttacgaatta ggcgagccgg tgtttgacgt gcgtgagtgc caaattcgtg
     4141 gtacaaccta cgctgcacca ttacgcgtga aattgcgttt agtctcttac gatcgtgaag
     4201 ccgcagccgg tacggtaaaa gacattaaag agcaaaacgt gtatatgggc gaaatcccat
     4261 taatgaccga taacggtact ttcgtgatta acggcaccga gcgtgttatc gtttctcaat
     4321 tacaccgtag cccaggtgtg ttcttcgatt ctgacaaagg taaaacccac tcttcgggta
     4381 aagtgcttta taacgcacgc attatccctt accgtggttc ttggttagat ttcgagtttg
     4441 atccgaaaga taacttattt gcccgtatcg accgccgtcg taaattgccg gcaaccatta
     4501 ttctacgtgc attaggctac accaccgaag aaatcttaaa tttattcttc gagaaaatta
     4561 aatttgaaat tgccgataat aaattattga tgacgttagt gcctgagcgt ttaagaggcg
     4621 agactgcatc atttgatatt gcagcaaacg gcaaagtata tgttgaaatg ggtcgccgta
     4681 ttaccgctcg tcatatccgt gcgttagaaa aagacaatgt gacccaaatt gaagtgccgg
     4741 tggaatatat tgtcggcaaa gtagcggctc aagattacgt tgatttatct tcaggtgaag
     4801 tgatttgccc ggcgaatatg gaaatctcga tggagatgtt ggcgaattta tcacaagccg
     4861 gttacaacca gatcgaagta ttatttacca acgatttaga ccacggtcca tatatttctg
     4921 aaaccttacg tgttgatcca acttacgacc gtttaagtgc gttggttgaa atctatcgta
     4981 tgatgcgtcc gggcgaacca ccaacgaaag aagcggcaga agcattattc gataatatgt
     5041 tcttctcggc agagcgttat gatttatcgg cagtaggtcg tatgaaattc aatcgctcgt
     5101 tagaaattcc ggaaggcgta ggcagcggta ttttaagcaa cgatgacatc atcggtgtga
     5161 tgaaaaaatt aatcgaaatc cgtaacggtc gtggtgaagt ggatgatatc gaccacttag
     5221 gtaaccgtcg tatccgcagc gttggtgaaa tggcagaaaa ccaattccgc attggtttgg
     5281 tgcgtgtaga acgtgcggtg cgtgagcgtt tatctttagg cgatttagat ggtgtaacac
     5341 cacaagattt aatcaacgct aaaccgattt cagcagcagt gaaagagttc tttggttcat
     5401 cacaactttc gcaatttatg gaccaaaaca acccgctttc agaggtaaca cataaacgcc
     5461 gtatctcggc attaggcccg ggtggtttaa cccgtgagcg tgccggcttt gaagtgcgtg
     5521 acgtacaccc gactcactat ggtcgtttat gtccaatcga aactcctgaa ggcccgaata
     5581 tcggtttgat caactcacta tcggtgtatg ctcgtactaa taactacggt ttcttagaga
     5641 ctccattccg taaagtggtg aatggtcaag taaccgaaga gatcgagtat ttatctgcga
     5701 ttgaagaaag caacaatatc attgcacagg cgaactcaaa cttagatgag aacttccgtt
     5761 ttaccgatac ttatgtaact tgccgtcagc aaggcgaatc gggcttattc aaaccggaag
     5821 aagttcaata tatggacatc tcaacccaac aagtggtttc tgtggctgcg gcgttaattc
     5881 cgttccttga gcacgacgat gcgaaccgtg cgttaatggg tgcgaacatg caacgtcagg
     5941 cagttccaac cttgcgtgcg gacaaaccat tagtcggtac cggtatggaa aaaccaattg
     6001 ctcttgactc aggtgtagcg attgtcgcaa aacgtggtgg tacggtgcag tatgttgatg
     6061 cgtcacgcat tgtagtgaaa gtaaatgaag atgaaaccat tccgggcgaa gcgggtatcg
     6121 atatttataa cttaatcaaa tacacccgtt caaaccaaaa cacctgtatc aaccaagtac
     6181 cttgtgtgaa cttgggtgag ccggttgctc gtggtgaaat cttagcggac ggtccttcaa
     6241 cagacttagg tgaattggca ttaggtcaaa atatccgtgt ggcatttatg ccttggaacg
     6301 gttataactt cgaagactca atgttagttt ctgagcgtgt ggtacaagaa gatcgcttta
     6361 ccactatcca cattcaagaa ttatcttgtg tggcgcgtga taccaaatta ggtgcagaag
     6421 agatcaccgc tgatattcca aacgtgggtg aatctgcatt aagcaaatta gatgaatcag
     6481 gtatcgttta catcggtgca gaagtgaaag gtggcgacat cttagtaggt aaagtaactc
     6541 ctaaaggtga aacccaatta acaccggaag aaaaactatt gcgtgcgatc ttcggtgaaa
     6601 aagcctctga tgtgaaagat tcatcattgc gtgttccaaa cggtacttca ggtacggtta
     6661 tcgatgttca agtatttacc cgtgatggcg tagagaaaga taaacgtgcg aaagaaatcg
     6721 aagagatgca actgtgtgaa gcgaagaaag acttagcgga agagttggaa atcttagaag
     6781 ccggtttatt tactcgtgtt cgcaacctat taattgaagg cggcgtaaaa gaatctgcat
     6841 tagatggcgt tgctcgtgaa aaatggttag aacaaaccct tgaagacgaa gcgaagcaaa
     6901 atcagttaga acagttagcg gaacagcacg aagagttacg caaagaattt gaacgtaaac
     6961 ttgagatcaa acgcaacaaa attatccaag gcgacgattt agcaccgggt gtgttaaaag
     7021 tagtgaaagt ttaccttgcg gtgaaacgcc aaatccagcc gggtgataag atggcgggtc
     7081 gtcacggtaa caaaggggtt atctcgaaaa tcaaccctgt ggaagatatg ccatacgatg
     7141 aaaacggtca gccggttgaa atcgtattga acccgctggg cgtaccgtct cgtatgaaca
     7201 tcggtcagat cttagaaacc cacttaggct tagcggctca tggtatcggt gatcagattg
     7261 ataagatgat caaacagcaa caagaagtgg ctaaattacg tgaatatatg caaaaagcct
     7321 acgatttagg tcacggctca caagtcgttg atttaagcac tttctctgat gaagaagtga
     7381 tgcgtttagc gcaaaactta cgcaaaggct taccgcttgc tactccggta ttcgatggtg
     7441 cacacgaaag cgaaatcaaa ggcttattag agttaggcgg cttaccgact tcaggtcaga
     7501 tcacacttta tgatggtcgt accggtgaga aatttgagcg tccggtaacc gttggttata
     7561 tgtatatgct caaattgaac cacttggttg atgataaaat gcacgctcgt tcaaccggtt
     7621 cttatagcct tgttactcaa caaccattgg gtggtaaagc tcaattcggt ggtcagcgtt
     7681 tcggtgagat ggaggtttgg gcattagaag catacggtgc ggcttatact cttcaagaaa
     7741 tgcttacggt gaaatcggac gatgtgaacg gtcgtacgaa gatgtataaa aacattgtag
     7801 atggtacaca ttatatggag ccgggtatcc ctgaatcctt taacgtaatt accaaagaga
     7861 tccgtgcatt agcaattgat atggagctgg acgaagcgta atggtttcac tctccccctt
     7921 gatgggggag agacaggcaa aggatttgtt cagaatcctt tggcagagag agggtgatgc
     7981 aagcggtgta aatttgctaa atttttgcaa aaatcgaccg cttaccctct ccctgatttt
     8041 gcttctgaac gaggcaaaat ctatccctct cccacaaggg gagagggttt aaaatcgcaa
     8101 ccaaattcgt agagtggata ttctttccac caaacaaaac aacctcaaac taggggcaaa
     8161 aaagtgaaag acttagtgaa gtttttaaaa gcacaaacaa aatcgaacga agattttgat
     8221 gtgattaaaa ttggtttagc atcaccggac aagatccgtt cttggtcttt tggtgaagtg
     8281 aaaaaaccgg aaaccattaa ctatcgtacc ttcaaaccgg agcgtgatgg tttattctgt
     8341 gcacgtattt tcgggccggt aaaagattac gagtgcttgt gtgggaaata caaacgttta
     8401 aaacaccgtg gcgtaatttg tgaaaaatgt ggtgttgaag taacccaaac taaagtacgt
     8461 cgtgagcgta tggggcacat tgaattagct tgccctgtcg cacacatctg gttcttaaaa
     8521 tcactaccgt cccgtatcgg tttaatctta gatatgccgt tgcgtgatat tgaacgtgtg
     8581 ctttattttg aatcttatgt agttactgaa ccgggaatga ccgatttaga gaaaaatcag
     8641 ttattaaccg aagaacaatt tatggacgct gaagagcgtt ggggtgatga atttgaagcg
     8701 aaaatgggtg cagaaggtat ccaagcatta cttcgtgatt tagatttaga acaccaatgc
     8761 gaaaccttgc gtgaagagtt acaagaaacc aattcagaaa ccaagcgtaa aaaaatcact
     8821 aagcgtttaa aaaccttaga agcattctta caatccggca acaaaccgga atggatggtg
     8881 atgacagtat taccggtact tccaccggat ttacgtccgt tagtaccgct tgacggtgga
     8941 cgttttgcga cttctgactt aaacgattta taccgccgtg taattaaccg taacaaccgt
     9001 ttaaaacgct tgttagattt agttgcaccg gacatcatcg tgcgtaacga aaaacgtatg
     9061 ttacaagagt cggttgatgc gttattagac aacggtcgcc gtggtcgtgc gattaccggt
     9121 tcgaacaaac gtccgttaaa atctttggca gatatgatca aaggtaaaca aggtcgtttc
     9181 cgtcaaaact tattaggtaa acgtgtagat tactcaggtc gttcggtaat taccgtaggc
     9241 ccatacttac gcttacacca atgtggttta ccgaagaaaa tggcgttgga attattccgt
     9301 ccgtttatct actcaaaatt agaaaaccgt ggcattgctt caactattaa agccgcgaag
     9361 aaaatggtag agcgtgaaga tcctatcgta tgggatattc ttgccgaagt gattcgtgaa
     9421 cacccgattc tacttaaccg tgcaccaaca cttcaccgtt taggtatcca agcgtttgag
     9481 ccgttattga ttgaaggtaa agcgatccag ttacacccac tcgtgtgtgc ggcgttcaat
     9541 gcggacttcg acggtgacca aatggcggtt cacgttccat taacccttga agcacagtta
     9601 gaagctcgtg cgttaatgat gtcaaccaac aacgtacttt caccggcaag tggtgatccg
     9661 attatcgtac cgtctcagga cgttgtactc ggtctttact atatgacccg tgagaaagta
     9721 aacggtaaag gtgagggaat gtacttcctt gaccctcgtg aagcggaaaa agcataccgt
     9781 accggtcagg ctgaattaca ttctcgtgtt aaagtgcgtg ttactgaata tgagaaaaac
     9841 gaagcaggcg aattagaagc aaaaacttcg ttagtagata ccaccattgg tcgttcaatc
     9901 ttatggatga ttgcaccaaa aggtatgccg tttagcttgt tcaaccaaac cttaggcaaa
     9961 aaagcgatct cgaaattaat taacgagagc tatcgccgtt taggtttaaa agaatctgtg
    10021 gttttagctg accaaattat gtacaccggc tttgcttatg ctgctcgttc tggtgcctca
    10081 gtaggtatcg acgatatggt aattccggag caaaaatacg caatcattga agcggctgaa
    10141 gcggaagtcg ctgagatcca agagcaattc aacggcggtt tagtaacagc cggtgagcgt
    10201 tataacaaag tgatcgatat ttgggctgct gcaaacgaac gtgttgcgaa agcaatgatg
    10261 gaaaaccttt caacggaaga agtaatcaac cgtgaaggta atccggaaaa acaagcctca
    10321 tttaacagta tctttatgat ggcggactcc ggtgctcgtg gttctgctgc acagattcgt
    10381 cagttagcgg gtatgcgtgg cttgatggct cgtccggacg gttcgattat cgaaacccca
    10441 attactgcaa acttccgtga aggtttgaac gtattacagt actttatttc aacccacggt
    10501 gcacgtaaag gtcttgccga tacggcatta aaaacagcga actccggtta cttaacccgt
    10561 cgtttagttg atgtggcaca agatttagtt atcattgaag atgactgtgg tacgcacgaa
    10621 ggtatcgtga tgacaccact tatcgaaggt ggtgatgtga aagaagccct gcgtgatcgt
    10681 gtattaggtc gtgtggtggc agaagatgtg ttaaaaccgg gtacagatga agtattaatt
    10741 ccacgcaaca ccttattaga tgaaaaatgg tgtgatgtgg tggatcgtga atctgtggat
    10801 gtgatcaaag tacgttcggt agtaacttgt gatacagact tcggtgtgtg tgctaaatgt
    10861 tacggacgtg acttggctcg tggtcactta atcaaccaag gtgaagcggt cggtgttatt
    10921 gcggcacaat ctatcggtga gccgggtaca cagttaacga tgcgtacgtt ccatatcggt
    10981 ggtgcggctt ctgcggcagc aaaagaatct agcatccaag tgaaaaacgc aggtacgatt
    11041 aaattaacca acgctaaatt tgtaaccaac aaagaaggca aaatcgtatt aacttctcgt
    11101 aatactgaat taactgtcat cgacaccttc ggtcgtacca aagaaaacta taaagtgcct
    11161 tatggtgcgg tgctttctaa acacgacggt gcagaagtgg ctgtgggtga agtggttgcg
    11221 aactgggatc cgcacacaat gccggtaatc tcagaggtat caggtcgtat ccaatttagc
    11281 gacatcgtag atggcttaac cgttactcgc caaaccgatg aattaaccgg tttatcatct
    11341 atcgtggtgc aagatgtggg tgaacgtgca accgcaggta aagatttacg tccggcatta
    11401 cgcttagttg atagcaacgg caaagacatc ttaattccgg gtacagacgt tgcagcacaa
    11461 tacttcttac cgggtaaagc aatcgtaacc ttagacgatg gtgcagaaat tgaagtcggt
    11521 gaagccttag cccgtattcc acaagaatct gtggggacga aagatattac cggtggtctt
    11581 ccacgcgtag cagacttatt cgaagcccgt aaaccgaaag agccggcaat cttggcggaa
    11641 atttcaggta ttgtgtcgtt cggtaaagaa accaaaggta aacgccgctt ggtaattact
    11701 ccggcggaag gcgaagcgtt tgaagagatg attccaaaat ggcgtcagct caacgtattt
    11761 gaaggcgaga tggtacaacg tggtgatgtg atatcagacg gtgcagaaac tccgcacgac
    11821 atcttacgct tacgtggcgt tcacgccgtt accgactaca tcgtaaacga agtgcaagaa
    11881 gtttaccgct tacaaggggt aaaaattaac gacaagcaca tcgaagtgat tgttcgccaa
    11941 atgttgcgta aagctgtgat caccaacgct tacgactctg aattcttaga gggcgaacaa
    12001 gtggaagtgg ctcgtgtgaa aattgccaac cgcaaacgtg ctgaagaagg caaaccgttg
    12061 gtagaatttg aacgtgaatt gcttggtatt accaaagcat ctcttgcaac agaaagcttt
    12121 atttctgcag cctcgttcca agaaaccact cgagtgctta ccgaagccgc agtggcaggt
    12181 aaacaagatg aattgcgtgg cttaaaagag aacgtaatcg ttggtcgctt aatcccggca
    12241 ggtaccggtt ttgcttacca ccaagcacga gctaaaaaac gtgcccaact ggaagcagga
    12301 attgcagttg tccctgaatt tgaagcaccg gcagctaaag ccaattcatt cgttaccgat
    12361 gcagacatcg aagctgaatt tgaatttgtt gcagacgatg caacccaaag tttagcagcg
    12421 ttgttaaatg cgggtgagga agagtaatag ctcgtagggt atg
//
LOCUS       DANLRK010000074        11990 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.074, whole genome shotgun sequence.
ACCESSION   DANLRK010000074 DANLRK010000000
VERSION     DANLRK010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 11990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11990
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.074"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            44..1042
                     /gene="yiaK"
                     /locus_tag="PXA33_001432"
     CDS             44..1042
                     /gene="yiaK"
                     /locus_tag="PXA33_001432"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005757429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HDL5120756.1"
                     /translation="MRVSYEELKAQFKRVLLARNVREEIAEECATVFADTTQAGAYSH
                     GVNRFPRFIQQLENGDIVPEAVPTKVLSLGAIEQWDAHQAIGNLTAKKMMDRAMELAS
                     ENGIGVVALRNANHWMRGGSYGWQAAEKGYIGICWTNALAVMPPWGAKECRIGTNPLI
                     IAVPTNPITMVDMSCSMYSYGMLEVHRLAGRQTFVDAGFDDDGNPTRDPATVEKNLRL
                     MPMGFWKGSGLSIVLDMIATLLSNGESTAAVTEDKDDEYCVSQVYIAIEVDRLIDGKT
                     KDEKLNRIMEYVKTAERADPDVAVRLPGHEFTQILADNKANGIPVDDTVWAKLQAL"
     gene            1167..1532
                     /locus_tag="PXA33_001433"
     CDS             1167..1532
                     /locus_tag="PXA33_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HDL5120757.1"
                     /translation="MFFGHISQVKANRYPPAIQIALNYLKNTDFDAMEAGVYELKGRQ
                     IYVQVLDLNTKSKHEFQPEVHRNYLDVQYLHRGKEIMAAAVDTGTNPIAMEYNPERDI
                     QYYQSVANENEFRCVEGNF"
     gene            1596..2075
                     /locus_tag="PXA33_001434"
     CDS             1596..2075
                     /locus_tag="PXA33_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HDL5120758.1"
                     /translation="MKALAQLVGKLLEILVVVILSAMSILVFVNVVLRYGFNSSISVT
                     EEVSRYMFVWLAFLGAVLAFSENQHVKVTMLTDKLSPTARNILSVVTDLAMLFCCYLI
                     VDGSWIQFNLNLNNFAPISGLPQGITFLASTVAGVLIGLLIIARIVATVGVIAKGEK"
     gene            2076..3353
                     /locus_tag="PXA33_001435"
     CDS             2076..3353
                     /locus_tag="PXA33_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017806296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HDL5120759.1"
                     /translation="MTVVIFLSVLLGSILLGIPVAFALLVCGVALMLHLDLFDAQILA
                     QQIVSGADSFSLMAIPFFILAGEIMNEGGLSKRIIDLPMKLVGHKRGGLGFVAILAAM
                     IMASLSGSAVADTAAVVAMLLPMMKTTGYPIDRSAGLIGTAGIIAPIIPPSIPFIVFG
                     VASGVSITKLFLAGIFPGIMMGICLAALWWWQAKRLDLMTFSKATKEELCISFKNSIW
                     ALLLPVIIIGGFRSGMFTPTEAGAVATFYALIVSLFVYREMKFKDLYKVVLAAAKTTA
                     VVMFLVAAANVTGWLITVAELPMMLTELLEPLIESPTTLLLVIMLAVFIIGMVMDLTP
                     TVLILTPVLMPLVEEAGIDPVYFGVLFILNTSIGLITPPVGNVLNVITGVSKLPFDQA
                     AKGVMPYLVMMICLLLLFVFFPSLVLVPLSWMQ"
     gene            3456..4442
                     /locus_tag="PXA33_001436"
     CDS             3456..4442
                     /locus_tag="PXA33_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HDL5120760.1"
                     /translation="MKLFNLKTLFALVAGVAVMSGSAMAETSLRFGYEAPRSDTQHIA
                     AKKFDELLAAKTKGEIKLKLFPDSTLGNAQTMISGVRGGTIDIEMSGSPNFTGLEPKL
                     NVIDIPFIFKDREHVYKVLDGEVGQGLLKDLEAQGLKGLAFWDVGFRAFSNSKHPVTK
                     PEDIKGLKVRTNQNPMYIQAFTLLGGNPVPMPLSELYTALETRAVDAQEHPIGIFWSA
                     KLFEVQKHLSLTNHGYTPLIVVMNKAKFDGLAPELQTALVEAAKEAGQFQRDLNVKNE
                     KEIIEKLRKQGIEVIEQVDNAPFKAAIEAEVRKAFVEKHGDELLKKVDALAQ"
     gene            4516..5499
                     /locus_tag="PXA33_001437"
     CDS             4516..5499
                     /locus_tag="PXA33_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HDL5120761.1"
                     /translation="MKLFNLKTLAAFVAGFALFSTAQAETSFRFAYEAPRSDTQHIAA
                     KKFNDLLQEKSQKGLKLSLFPDSTLGNAQTAISGVRGGTIDIAMSSSSNFTGLEPKLN
                     VIDIPFIFKDRDHAYKALDGEIGQGLLTALEAHGLKGLAFWEVGFRAFSNSKHPITKP
                     EDIKGLKIRTNQNPMYIQAFSILGANPVPMPLSKLYTALETKAVDAQEHPIGIFWSAK
                     LYEVQKYFSFTNHGYTPLIVAINKAKFDALPAEQQTALVEAAKEAGKYQRQLNLENEQ
                     KIIDSLKKAGIEFVDNLDTTPFKDAVSAETRKAFIEKNGDGLVKAIDALAK"
     gene            5630..7114
                     /locus_tag="PXA33_001438"
     CDS             5630..7114
                     /locus_tag="PXA33_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDL5120762.1"
                     /translation="MQHIIKRFGSHTAVNDVSLDIHSGEIVALLGENGAGKSTLIKIL
                     AGIYDRDEGDILFHNQKIQSAHAIEKGNKKPIAFIHQDLGLIEWMTIAENMAFVMGFP
                     RKFGLIDWAKVREQSQKALDFVGIKLSADTRVFDLSRTEKALLAIARAIAVNAEILVL
                     DEPTASLPASDVEHLFTVLNRLRSQGVGMIYVTHRLDEVIAISDRILVMRDGFPVAEG
                     QTKLYDVKGLVKAIVGEESRGRQRVDLPTDTPEILSLQNVVVGDTGPVNFRLHKGEMI
                     ALAGLRGAGQEEIGRLLFGERTLDKGEIQLDKRSYSAKDPQDAIAKGVALVAGDRTRE
                     SVFMSMTTSENLFINPVLSGHSPLKRYSRREEWGESWYKFQLFDIRPKNLFIDASALS
                     GGNQQKIVLARWMHLNTPVLILEDPTAGVDVGARAEIYDLLNKALAEGVAIIVISTDF
                     EEIAHLCNRALVFNRGEIAGELLNEEVTFANLLALASDSQSKKQ"
     gene            7309..8355
                     /locus_tag="PXA33_001439"
     CDS             7309..8355
                     /locus_tag="PXA33_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL5120763.1"
                     /translation="MAQTNIKSTALEQPVSIARDGFMAWFSQMSTRYGLLWLCVLLVI
                     IFSLTTESFASMLTLNAILESKSKIALLALAATTTMIVGKIDLNVGFGIVLWHILVIT
                     LQVQYGFSWYMAILIVLVIAALYGLLNGILVALADIDSFVATLGSGTVLYAVALWHSG
                     GRQIVGDLPDEFIALNSTEIFGIPISAFYVLAVAIVMWLVTEHTPTGRCMYAVGGNPT
                     AARLNGINIKKYTIVPFIVSSVITAFTGVLISAQQGVGQASVGMDYLLPALVGAFLGS
                     TTIRPGRINVWGTVVGIAILAIGISGIQQFGGAFWVEPLFNGATLLLSITIAGYAQRK
                     RLLNQKAVQKKSTN"
     gene            8368..9477
                     /locus_tag="PXA33_001440"
     CDS             8368..9477
                     /locus_tag="PXA33_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005757420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDL5120764.1"
                     /translation="MKKAFRTTLTAIATLTAIAFGNNAFANDDPFVQEAKQLVAAATA
                     KQEKWDGPTTGPQLQKEKTVIFIASDMKNGGVLGVIDGMKEAVGVTGWKLDVLDGAGT
                     VNNQLAALNQAIARKPDAIVIGGWNPNVAKIPLQKATKNGIKLAAWHATPEPGAIDKY
                     GIFYNVTSDSDEIARLSAMLAVAESNGKAKAIILTDSLYEIALRKANVMKEVIEKCKE
                     CKVLEFIDTPLADTSSRMPNLTFSLLQKYGDDLEYALAINDLYFDFMAPSLRTAGKDK
                     PFSISAGDGSVTAYQRIRNGDKQFATVPEPLNLHGWQLVDELNRAFANEKPSGYITPA
                     HLVTKENVAFDGGENNLYAPQNGYKGAYKAIWGVK"
     gene            9582..10472
                     /locus_tag="PXA33_001441"
     CDS             9582..10472
                     /locus_tag="PXA33_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HDL5120765.1"
                     /translation="MSNKFIPKISNPRFNELVGNTFELEELFDKAIWAEGPAWSKPQQ
                     ALYFSDVKGCTMYRWTEQDGTQVFRQPSYFSNGNAFDANDNLITCEHGNRGISLTTPS
                     GEYRFLVGQLDGKRLNSPNDVVVKSDGIIWFTDPPYGILSDNEGKQSPSEIIGCYIYC
                     YDPKTDKLNIATFNTMRPNGLAFSSDEKQLFVADMSSVEFDEDGLHHLVVFDVDGNKL
                     KNRRDVAEISPGIPDGFRIKQNGVIFCSCEEGIIALLPDGTELGRFVLGKLTSNCSFG
                     DDEQTLFITCSNSVYRLTIK"
     gene            10544..>11990
                     /locus_tag="PXA33_001442"
     CDS             10544..>11990
                     /locus_tag="PXA33_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005610664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HDL5120766.1"
                     /translation="MDNKMNYTLGIDCGGTFIKAALFDTQGNIQALHRENVSVISEQA
                     GYAERDMSQLWAICAEVVRSTIEKSGVNPADIKAVGISAQGKGAFLLDKQNRPLGRAI
                     LSSDQRSLEIVKQWQAEGIPQQLYPITRQTLWTGHPVSILKWVQQNEPERYAQIGAVL
                     MSHDYLRFCLTGELHCEETNISESNLYNMEKGEYDQQLADLLGMPDVLEKLPPVIEPN
                     KISGYVTENAAKLTGLAVGTPIVGGLFDVVSTARCANLDDETKLNAVLGTWSVVSGIT
                     DHIDASQSLPFVYGRYAEAGKFIVHEASPTSAGNLEWFVNQWKLDYVKINQAVAQLPP
                     AASSVLFVPFLYGSNAGLGMQAGFYGMQAHHTQAHLLQAIYEGVLFSLMSHLNRMFIR
                     FPKADVLRVTGGPVKSKVWMQMLADLTGMRLEIPQMEETGCLGAALMAMQGVGLDISQ
                     VQALNNEMQVFEPNPENYAAYQTKYQRYQKLT"
BASE COUNT         3319 a         2591 c         2903 g         3177 t
ORIGIN      
        1 acgtgatttt actatttgaa atattgaggt attcaagatg actattcgag tttcctatga
       61 agaattaaaa gctcaattta aacgtgttct gttggctcgc aatgtgcgtg aagagattgc
      121 cgaagagtgt gccactgtgt ttgccgacac cactcaagcg ggtgcttatt cacacggggt
      181 aaaccgtttc ccacgcttta ttcagcagtt agagaacggt gatattgtgc cggaagccgt
      241 gccgactaaa gtgctttcac tgggggcgat tgagcagtgg gacgctcacc aagccatcgg
      301 taacttaacc gcaaaaaaaa tgatggacag agcaatggag cttgcatctg aaaacggcat
      361 cggcgtagtg gcgttacgta atgcaaacca ctggatgcgt ggcggttctt acggatggca
      421 ggcggcagag aaaggctata tcgggatctg ctggacgaac gcccttgcgg taatgccgcc
      481 ttggggggcg aaagagtgcc gtatcgggac taacccgtta atcattgcgg tgccgactaa
      541 cccaatcacg atggtggata tgtcttgctc aatgtactca tacggaatgt tggaagtgca
      601 tcgccttgct ggtcgtcaaa cctttgtcga tgctggtttt gatgatgacg gcaacccaac
      661 ccgtgatccg gcaacggtcg agaaaaacct ccgtctaatg ccgatgggct tctggaaagg
      721 ttcgggctta tcgattgtgt tagatatgat cgccaccttg ctctctaacg gtgaatccac
      781 tgcggcagtc accgaagata aagacgatga atattgcgtg tcgcaagttt atatcgctat
      841 tgaagtggat cgcttaattg acggcaaaac caaagacgaa aaactcaacc gtattatgga
      901 atatgtgaaa accgcagaac gtgccgaccc tgatgtggca gtacgtttac cgggccacga
      961 gttcacccaa attctagccg ataacaaagc caacggcatt ccggtggatg acaccgtttg
     1021 ggcgaaatta caggcgttgt aattgcaaat tgaccggtag ggtgggcgta agcccacgca
     1081 ttagacacaa tcataagatg acgtgggctt acgcccaccc tacaagcggt catttttccg
     1141 cagaatttta caaaacggag gtatgaatgt tttttggtca tatttcacaa gttaaagcga
     1201 atcgataccc gccggcgatc caaattgcgt tgaactatct gaaaaatacc gattttgatg
     1261 cgatggaagc aggggtttac gaactcaaag gcagacaaat ttatgtgcag gtgttggatt
     1321 tgaacaccaa atcgaaacac gaatttcagc cggaagtgca tcgcaattac ctcgatgtgc
     1381 agtatctaca ccgaggcaaa gagataatgg ctgcggcggt ggataccggc accaatccga
     1441 ttgcgatgga atacaaccct gagcgggata ttcaatatta tcaatccgtt gccaacgaaa
     1501 atgaatttcg ctgcgttgaa ggcaattttt gacggcacgg aaaccattcg caaagtggtg
     1561 gtaaaaattg cgatgagcga aattgaggag caacgatgaa agccttagct caattagtcg
     1621 gaaaactgtt agaaatcttg gtggtggtga ttttatccgc aatgtcgatt ttggttttcg
     1681 tcaatgtggt actacgttac ggttttaaca gcagcattag cgtgaccgaa gaggtgtctc
     1741 gctatatgtt tgtttggttg gcgtttttag gggcggtgtt ggcatttagc gaaaatcaac
     1801 acgttaaagt cacaatgctg accgataaat tatcgccaac ggctcgtaat atcttaagcg
     1861 tagtcacaga tttagcgatg ttgttctgtt gctacttaat tgtggacggt agctggattc
     1921 aatttaacct caacctgaat aactttgccc cgatttcagg cttaccgcaa ggcatcactt
     1981 tccttgccag cacagtggca ggggtattaa tcggtttatt aattattgcc cgcattgtgg
     2041 caacggttgg cgtgattgca aaaggagaaa aatagatgac tgtggtaatt tttctttccg
     2101 tcttgcttgg ctcgatttta ttgggcattc cggtagcttt cgccctgttg gtctgcggtg
     2161 tggcgttaat gttgcattta gatctgttcg atgcccaaat tttggctcaa caaattgtga
     2221 gcggtgcaga cagtttctcg ctaatggcaa ttccgttctt cattcttgcc ggtgaaatta
     2281 tgaacgaggg cggtctctcc aaacggatta tcgacctgcc gatgaaatta gtcggtcata
     2341 aacggggcgg tttaggtttt gtagcaattt tagcggcgat gattatggca agcctttccg
     2401 gttcggcggt ggcggatacc gcagcggtag tcgcaatgct actgccgatg atgaaaacca
     2461 ccggttatcc gattgaccgc tctgccggtt taatcggtac ggcagggatt atcgccccaa
     2521 tcattccgcc aagcattccg tttatcgtat ttggtgtggc tagtggcgtg tccattacca
     2581 aactgttctt agccggtatt ttcccgggca ttatgatggg gatctgcttg gctgcccttt
     2641 ggtggtggca ggcaaaacgt ttggatttaa tgaccttctc caaagcgacc aaagaagaac
     2701 tttgcatttc atttaaaaac agtatttggg cgttattgtt gcctgtcatt attatcggtg
     2761 gtttccgctc gggaatgttc accccaaccg aagcgggtgc ggtcgcaact ttctatgcct
     2821 tgattgtttc actgtttgtt taccgtgaaa tgaagttcaa agatttatac aaggtagtgt
     2881 tggcggcagc gaaaaccacg gcagtagtga tgttcttagt ggcagcggca aatgtgaccg
     2941 gctggctgat taccgtagcg gaattaccga tgatgctcac cgagctgtta gagccgttaa
     3001 ttgaaagccc gaccacactc ttattggtga ttatgttagc ggtgtttatt atcggaatgg
     3061 tgatggactt aaccccaacc gtgctgattt taacgccggt attaatgccg ttggtggaag
     3121 aggcaggcat tgacccggtt tacttcggtg tgctgtttat tcttaacacc tccatcggct
     3181 tgattacacc accggtcggc aacgtgctaa acgtgattac cggcgtgtcg aaactgccgt
     3241 tcgaccaagc ggctaagggc gtaatgcctt acctcgtaat gatgatttgt ttactactgt
     3301 tgttcgtgtt cttcccgtca ctggtgttag tgccgttaag ctggatgcag taatggtttg
     3361 aaccgaaaca agcggtcgaa ttgacgcaaa aatttgcaaa tttttttggc aaaacagacc
     3421 gcttgcaact caatttttaa tcacaggaga accctatgaa attatttaat ttaaaaacct
     3481 tatttgcctt agtggcaggc gttgcggtaa tgagcggctc tgcaatggca gaaacctcac
     3541 tgcgttttgg ttatgaagcc ccacgttccg atacccaaca catcgccgcc aaaaaattcg
     3601 atgaattatt ggcagcaaaa accaaaggtg aaattaaatt aaaactgttt ccggacagca
     3661 ccttaggtaa tgctcaaacg atgatcagcg gcgtgcgtgg cggtactatc gacattgaaa
     3721 tgtccggctc gccaaacttt accggcttag agccaaaact aaatgtgatt gatattccgt
     3781 ttattttcaa agatcgtgaa cacgtttaca aagtattaga cggcgaagtt ggtcaaggct
     3841 tattaaaaga tttagaagcc caaggcttaa aaggcttagc cttctgggac gttggcttcc
     3901 gtgctttctc caactcaaaa caccctgtga ccaagccgga agacatcaaa ggcttaaaag
     3961 tccgcaccaa ccaaaacccg atgtacattc aagccttcac cttactaggc ggcaacccgg
     4021 tgccaatgcc actttctgag ctttacaccg cactcgaaac ccgtgccgtt gatgcccaag
     4081 aacacccaat cggcattttc tggtcggcaa aactgtttga agtacaaaaa catttaagtt
     4141 taaccaacca cggctacacc ccgttaatcg tggtgatgaa caaagcgaag tttgacggct
     4201 tagctcctga attacaaacc gcattagttg aagccgcaaa agaagccggt caattccaac
     4261 gtgatctcaa cgtgaaaaac gaaaaagaga tcatcgaaaa attacgcaaa caaggcatcg
     4321 aagtgatcga acaagtggat aacgccccgt tcaaagcagc gattgaagcg gaagtccgca
     4381 aagctttcgt ggaaaaacac ggcgatgaat tattgaaaaa agtggatgcg ttagcacagt
     4441 aattgacttt attttagtct aaagctccct ccgtttgggg ggagttgttg tatagactct
     4501 actataggag agactatgaa actgtttaat ttaaaaactt tagccgcttt cgttgccggt
     4561 tttgcactgt tttcaaccgc acaagcagaa acctcattcc gctttgcgta tgaagctcct
     4621 cgttctgata ctcaacatat tgcagcgaaa aaatttaatg atttgctgca agaaaaatcc
     4681 caaaaagggc tgaaattaag cctcttccct gatagcacgc tcggcaacgc acaaacggcg
     4741 attagcggtg tgcgtggcgg tactattgat attgcaatgt ccagctcctc taactttacc
     4801 gggttagaac caaaactgaa tgtgattgat attccgttta ttttcaaaga cagagaccac
     4861 gcctataaag ccttagatgg agagattggt caaggtttat taaccgcatt agaggctcac
     4921 ggtttgaaag ggttggcgtt ttgggaagtt ggtttccgag cgttttccaa ctccaagcac
     4981 ccgatcacca aaccggagga tattaaaggc ttaaaaatcc gtactaacca aaacccgatg
     5041 tatattcaag ccttctctat tttaggtgca aatccggtgc caatgccgct ttccaagctt
     5101 tacactgcac tggaaaccaa agcggttgat gcccaagaac acccaatcgg cattttctgg
     5161 tcggcaaaac tctatgaagt acaaaaatat ttcagcttta ctaaccacgg ttacaccccg
     5221 ttaattgtgg cgattaacaa agcgaaattt gatgcactgc cagccgagca acaaaccgca
     5281 ttagttgaag cggcaaaaga agccggaaaa taccaacgcc aacttaacct agaaaatgag
     5341 cagaaaatta tcgactcgct caaaaaagca ggtattgaat ttgtggataa cttagatacc
     5401 acaccgttca aagatgccgt atcggctgaa acccgcaagg catttatcga gaaaaacggt
     5461 gatggcttag ttaaagccat tgatgcattg gcgaagtaaa aatagagcta caaagtgctc
     5521 ggttaaacta acctcacacg aatcgtgtga ggtacaagcg gtcaaaatcc gcaaaatttt
     5581 tacacatctt tcctcaaagg aggaacaaat ggcacagccc attttaagta tgcagcacat
     5641 catcaaacgc tttggtagcc atacggcggt gaatgatgtc agccttgata tccactcagg
     5701 cgagatcgtt gccttactcg gtgaaaacgg tgcaggtaaa tcgaccctaa tcaaaatcct
     5761 tgccggtatt tatgaccgtg acgagggtga tattcttttc cataaccaaa aaatccaatc
     5821 tgcccacgct attgagaaag gcaacaaaaa gcccatcgct tttatccacc aagatttagg
     5881 cctaatagaa tggatgacga ttgccgaaaa tatggcattt gtaatggggt tccctcgcaa
     5941 atttggcttg attgactggg cgaaagtgcg agagcagtcg caaaaagccc ttgattttgt
     6001 aggcattaaa ctctctgccg acactcgagt gtttgattta agccgtaccg aaaaagcctt
     6061 actcgccatt gcccgtgcga ttgccgtcaa tgcagaaatc ttggttttag acgaacctac
     6121 tgcttcccta cctgcctctg atgtagagca tcttttcact gtgttaaacc gcttacgttc
     6181 ccaaggtgtg ggaatgattt atgtgaccca ccgtttagat gaagtgatcg ccatttccga
     6241 ccgcattttg gtgatgcggg acggtttccc ggttgcagaa gggcaaacca agctctatga
     6301 cgtcaaaggc ttggtcaaag cgattgtagg cgaagagagc cgtggcagac agcgggtgga
     6361 tttaccaacc gatactcctg aaattttaag cctgcaaaat gtcgtggttg gcgacaccgg
     6421 cccggtgaat ttccgtctgc acaaaggtga gatgattgcc cttgccggct tgcgtggagc
     6481 agggcaggaa gaaatcggcc gcttattatt tggggagcga accttagaca aaggcgaaat
     6541 tcagttagac aagcggtcat attctgctaa agatccgcaa gatgcgatag ccaaaggtgt
     6601 ggcattggtt gcgggagacc gcacccgtga aagcgtgttt atgtcaatga ctaccagtga
     6661 gaacctgttt atcaacccgg tactaagcgg tcattcacca ttaaaacgct actcccgccg
     6721 tgaagagtgg ggtgagagct ggtacaaatt ccaactattc gatattcgtc cgaaaaattt
     6781 gtttattgat gccagtgcgt tatccggtgg taatcagcag aaaatcgtgt tggcacgttg
     6841 gatgcacctc aatacgcctg ttctgatttt agaagacccg actgccggcg tggatgtagg
     6901 agcaagggca gagatttacg acttgctcaa caaagccctt gcagaagggg tcgcaattat
     6961 tgtgatttcc accgactttg aagagatcgc tcatttgtgt aaccgagcat tagtcttcaa
     7021 ccgaggggaa attgccggtg aactactgaa cgaagaggtg actttcgcca atttgctggc
     7081 gttggcatcg gatagtcagt cgaaaaaaca ataacttact ctcgcccgct tgcgggagag
     7141 agacagctaa aaatagcgtc aggctatttt tagcagagag agggtaaaca agcggtcatt
     7201 tttcttaaaa aatattgcga accctctccc tccgaaagcc taacggcttt ctcctccctc
     7261 tcccgcaagc gggcgagggg aaaatgcaga attttaacgg agtaatttat ggctcaaaca
     7321 aacatcaaat caaccgcact ggaacaaccg gtatctatcg cccgtgacgg ttttatggca
     7381 tggttttcac aaatgtccac tcgttatggc ttactgtggt tatgtgtgtt attggtgatt
     7441 attttctcac ttaccaccga atctttcgcc tcaatgctga cattaaatgc gattttagaa
     7501 agtaaatcga aaattgccct acttgctttg gcagccacaa ctacaatgat tgtcggcaaa
     7561 atcgacctga acgtgggttt cggtattgtg ttgtggcaca ttcttgtgat tactctgcaa
     7621 gtccaatacg gcttctcgtg gtatatggcg atcttaatcg tgttagtgat tgccgctctt
     7681 tacggcttgc tgaatgggat tttagttgct cttgctgata tcgatagctt tgtggcaaca
     7741 cttggctcag gcacagtgct ttatgccgta gcactctggc actcgggcgg tcgccaaatt
     7801 gtcggggatt tacccgatga atttatcgca ctcaacagca cagaaatttt cggtatcccg
     7861 atttctgctt tctatgtgct ggcagtggct atcgtaatgt ggttggtgac agaacatacc
     7921 ccgaccggtc gttgtatgta tgcggttggt ggcaacccaa cggcagctcg cctaaatggt
     7981 atcaatatta aaaaatacac cattgtgccg tttattgtct ctagtgtaat cactgctttc
     8041 acaggcgtat tgatctctgc tcaacaaggc gttggtcagg caagtgtcgg tatggactac
     8101 ttactgcccg cattggttgg ggcattctta gggagtacca ctattcgccc agggcgtatt
     8161 aacgtatggg gaacagtggt ggggattgcc attcttgcca tcggtatttc aggtattcaa
     8221 caattcggtg gagcgttctg ggttgagcca ctctttaacg gtgcaacact gttactttcc
     8281 atcactattg caggctatgc ccaacgcaaa cgcttgttaa atcaaaaagc tgtacagaaa
     8341 aaatcgacca actaagagga tttgattatg aaaaaagcat ttcgtacaac cttaactgca
     8401 atcgcaacct taactgctat tgctttcgga aataatgcgt ttgccaatga cgacccgttc
     8461 gtacaagaag caaaacagct tgtcgcagca gctacggcta agcaagaaaa atgggacgga
     8521 ccgacaaccg gccctcaatt acaaaaagag aaaactgtga tttttattgc ctctgatatg
     8581 aaaaacggtg gtgtacttgg tgtgattgac ggaatgaaag aagctgttgg cgtaacgggc
     8641 tggaagttag atgtattaga cggtgcaggt acggtaaaca accaactcgc cgcacttaac
     8701 caagcgattg cccgcaaacc ggatgcgatt gtgattggtg gctggaaccc gaatgtggcg
     8761 aaaatcccac tacaaaaagc gactaaaaac ggcattaaac ttgcagcttg gcacgcaaca
     8821 ccggagccgg gtgcgattga taaatacggc attttctaca acgtgacctc tgactcagac
     8881 gaaattgcac gcttatcggc aatgcttgcg gtggcagaat ctaacggtaa ggcaaaagcg
     8941 attattttaa ccgactcgct atatgaaatt gcgttacgca aagcgaacgt catgaaagaa
     9001 gttatcgaaa aatgtaaaga gtgtaaggtg ttagagttta tcgacacgcc attggcggat
     9061 acctcaagcc gtatgcctaa cctcactttc agcttactgc aaaaatatgg cgacgattta
     9121 gagtatgcgt tggcaattaa cgatctctac tttgacttta tggctccgtc tttacgcacc
     9181 gctggtaaag ataaaccgtt tagcatttcc gcaggtgatg gctcagtaac ggcttatcaa
     9241 cgtattcgca atggtgataa acagtttgca accgtccctg agccacttaa cttacacggc
     9301 tggcagttag tcgatgagct aaaccgtgct ttcgctaacg aaaaaccgtc tggttatatc
     9361 acaccggctc acctagtgac taaagaaaat gtggcgtttg acggtggtga aaacaacctg
     9421 tatgccccac aaaacggtta caaaggcgct tacaaagcga tttggggcgt gaagtaatga
     9481 cgtaggggca aataaatatt tgcctcgaac aagcggttgt tttttgcaaa gattttgcga
     9541 aatttaaccg cttgtcataa aaaatagaga gggtaataca aatgagcaat aaatttatcc
     9601 ccaaaatctc caaccctcgt tttaatgagc tggttgggaa tacgtttgaa cttgaagagc
     9661 tttttgataa ggcaatctgg gcagaagggc cggcgtggag caagccacaa caagccctct
     9721 attttagtga tgtaaaaggt tgcacaatgt accgctggac agagcaagat ggtacacagg
     9781 ttttccgcca gccgtcttat ttttctaacg gcaatgcgtt tgatgcaaac gacaacttga
     9841 ttacctgtga acacggcaat cgaggtatca gtttaaccac gccaagcggt gaatatcgtt
     9901 ttttggtcgg tcaattagac ggaaaacgac tgaactcacc caatgatgtg gtggtaaaat
     9961 cagacggcat catttggttc actgatccac cttacggtat tttgtcggat aacgaaggca
    10021 aacagtcgcc aagcgaaatt atcggttgtt atatttactg ctatgatccg aaaaccgaca
    10081 agctgaatat tgcaactttc aacacgatgc gaccaaacgg cttggccttc tcgtcagacg
    10141 aaaaacaact ctttgtggca gatatgtcga gcgtggaatt tgacgaagat ggcttacacc
    10201 acttagtcgt atttgatgtg gacggtaata aactaaaaaa tcgccgagat gtggcggaaa
    10261 ttagtcccgg cattccggac ggcttccgaa ttaaacaaaa cggcgtaatt ttctgctctt
    10321 gtgaagaggg aattattgct cttttacctg acggcacgga gctgggacga tttgtgctgg
    10381 gtaagctaac ctcaaattgt agctttggcg atgatgaaca aacactgttt attacttgtt
    10441 cgaatagcgt ttatcgttta acaattaaat agcagaatgc attaagtgcg tctgttgtag
    10501 cattacaagc ggtttctttt tcaaaatttt ttacaaacaa ataatggata acaaaatgaa
    10561 ctacacactc ggcattgatt gcggtgggac ttttatcaaa gccgctcttt ttgatacaca
    10621 aggtaatatt caagcccttc atcgtgaaaa cgtttcggta atcagtgaac aggcaggtta
    10681 tgcagagcgt gatatgtcac aactctgggc tatttgtgcg gaagtagtgc gttcaaccat
    10741 tgaaaaaagt ggggtaaacc cagcggatat taaagcagta gggatttccg ctcaaggcaa
    10801 aggggcgttt ttactggata agcagaatcg accgttgggg cgtgcgattt tatcttccga
    10861 ccaacgttcc cttgagattg ttaaacaatg gcaggctgaa ggcattccgc aacaacttta
    10921 cccgattacc cgccaaactc tttggacagg acaccctgtg tcgattttga agtgggtgca
    10981 gcaaaatgaa ccggagcgtt atgcacaaat cggggcggtg ttgatgtcgc acgactattt
    11041 gcgtttttgc ttaaccggtg agttgcattg cgaagagacc aacatttccg aaagcaatct
    11101 ttacaatatg gaaaaaggcg aatacgacca acagttagcc gatttgttgg gaatgccgga
    11161 tgtgttggag aaattaccac cagtgattga accgaataaa atttcgggat atgtgactga
    11221 aaatgccgcc aaattaaccg gtttagcggt gggtacgccg atagtcggtg gcttgtttga
    11281 tgtggtttct accgcacgtt gtgccaattt agatgacgaa accaaactga atgcagtgct
    11341 gggaacgtgg tcggtggtga gcggcattac cgatcatatt gatgcaagcc aatcattacc
    11401 ttttgtttat gggcgttatg ccgaggcagg gaaatttatc gtccacgaag caagcccgac
    11461 ttctgccggt aatttagagt ggtttgtgaa tcagtggaaa ctcgattatg tgaaaatcaa
    11521 ccaagctgtt gcccaattac cgccggcggc aagctcggtg ctgtttgtgc cgtttctgta
    11581 tggctcaaat gcaggcttag gaatgcaagc cggtttctat ggtatgcaag cccaccatac
    11641 ccaagcccat ctcttgcagg caatttacga aggagtgttg tttagcttga tgagccattt
    11701 aaaccgtatg tttatccgct ttccaaaggc tgacgtgctg cgagttaccg gcggaccggt
    11761 gaaatctaaa gtttggatgc aaatgttggc cgatttaacc ggaatgcggc ttgaaatacc
    11821 acaaatggaa gaaaccggct gcttgggtgc ggcattaatg gcaatgcaag gggttgggct
    11881 ggatataagc caagttcaag ccctaaataa tgaaatgcag gtgtttgagc caaatccgga
    11941 aaattatgcg gcttatcaga cgaaatatca gcgttaccaa aaattaactg
//
LOCUS       DANLRK010000075        11924 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.075, whole genome shotgun sequence.
ACCESSION   DANLRK010000075 DANLRK010000000
VERSION     DANLRK010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 11924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11924
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.075"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            285..1649
                     /locus_tag="PXA33_001443"
     CDS             285..1649
                     /locus_tag="PXA33_001443"
                     /EC_number="2.6.1.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005820549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HDL5120767.1"
                     /translation="MTIKTPIQAVLASNQYFLDRQDAMESNVRSYPRKLPFAYQKAQG
                     VWVTDVEGNEYLDFLAGAGTLALGHNHPKLIQAVKDVLDSGLPLHSLDITTPLKDAFT
                     EELLSFFPKDKYILQFTGPSGADANEAAIKLAKTYTGRGNVIAFSGGFHGMTHGALSL
                     TGNLSAKNAVQGLMPGVQFMPYPHEYRCPFGIGGEAGAKAVEHYFENFIEDVESGVVK
                     PAAVILEAIQGEGGVVPAPISFLQKVREVTQKHGILMIVDEVQAGFCRSGKMFAFEHA
                     GIEPDIVVMSKAIGGSLPLAVLAIKKEFDAWQPAGHTGTFRGNQLAMATGYVSLKIMR
                     EENLAKNAQERGEYLTNALRELSKELPCIGNVRGKGLMMGIDIVDERQPKDATGAYPR
                     DCDLAVAIQKYCFKNKLLLERGGRGGNVVRVLCAVNITQAECEEFIKRFKQSVAEAVK
                     AVRG"
     gene            1739..3274
                     /gene="ddc"
                     /locus_tag="PXA33_001444"
     CDS             1739..3274
                     /gene="ddc"
                     /locus_tag="PXA33_001444"
                     /EC_number="4.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005693296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2,4-diaminobutyrate decarboxylase"
                     /protein_id="HDL5120768.1"
                     /translation="MSNLEKHRQSLFCSDPKSIADYESAMNNAVKAVSDWLKEEKMYT
                     GGSIKELRAKINGFNPTQAGVGVQKSLEHLVEIFLKPSLKVHHPHSLAHLHCPTMVTS
                     QIAEVLINATNQSMDSWDQSPAGSIMEEHLIDWLRQKVGYGKGTSGVFTSGGTQSNLM
                     GVLLARDWAIANHWKKADGSEWSVQADGIPADAMQKVKVVCSENAHFSVQKNMAMMGM
                     GFQSVVTVPSNANAQMDVEALAKTLADLKAEGKIVACIVATAGTTDAGAIDDLKAIRK
                     LADEHQAWVHVDAAWGGALLLSKDFRHFLDGIELTDSITLDFHKHFFQSISCGAFLLK
                     DEANYRFIDYKADYLNSEYDEEHGVPNLVSKSLQTTRRFDALKLWFTVEALGEELYAS
                     MIDHGVYLSKEVEAYINATDGLEMLVPAQFASVLFRVVPQGYPAEFVDTLNQNVADEL
                     FARGEANIGVTKVGEHQSLKMTTLSPIATLENVRALLAQVLAEADRIKDSIVNGTYVP
                     PID"
     gene            3377..3739
                     /locus_tag="PXA33_001445"
     CDS             3377..3739
                     /locus_tag="PXA33_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015586988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NirD/YgiW/YdeI family stress tolerance protein"
                     /protein_id="HDL5120769.1"
                     /translation="MKKVLGLTLMAMALSACNSTASNGSSQANTGVAPANVISVKQAL
                     AAADDSYVTVEGTLLNKVGDETYLFKDATGQIRVEIDDEVWAGQHVGSEGNKVRLYGE
                     IDKGFNKTELDVKTLTVL"
     gene            complement(3829..4416)
                     /locus_tag="PXA33_001446"
     CDS             complement(3829..4416)
                     /locus_tag="PXA33_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDL5120770.1"
                     /translation="MNHLIIYAHPNPSSFNHAILEKTIAASVPNKVVVRDLYQLNFKP
                     ILDWAEFQGTLSKHYEADVAIEHQYWLEADVITLIYPLWWMGFPAILKGYLDRVLSYD
                     FAYRNGEIESIGLLTGKKMQQFVTLGNSNEKYERKGFLAAFEHTLGKGLFEFCGIRDV
                     KMHYFGNIGLKETDYTAILQQITASCSTILKEQAV"
     gene            4592..5140
                     /gene="napF"
                     /locus_tag="PXA33_001447"
     CDS             4592..5140
                     /gene="napF"
                     /locus_tag="PXA33_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HDL5120771.1"
                     /translation="MTDYPNQSLPRRSFLRGQFLDSLKSQKVSQQGYEPIRPPWANLA
                     NFLEKCTACGNCIQACETQIIVKGAAGYPEIDFSRGECTFCEKCVHSCEAEVFRAVSE
                     QPWSHKIEIQAGCLLKLGTECRSCGDSCEMRVIRFRPSLGGIAEMVLNLESCNGCGAC
                     LSVCPTSAIKIQPLQTQANRDE"
     gene            5133..5399
                     /locus_tag="PXA33_001448"
     CDS             5133..5399
                     /locus_tag="PXA33_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HDL5120772.1"
                     /translation="MSEKLSEQENWYVCSLVVQAKPEKLEEVKAEILKIPHTEIHGEK
                     TDEGKLVVTIESNVHLALSDRIDQVRDIKGVIVVSLISNYIDEQ"
     gene            5438..7936
                     /gene="napA"
                     /locus_tag="PXA33_001449"
     CDS             5438..7936
                     /gene="napA"
                     /locus_tag="PXA33_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005566990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase catalytic subunit NapA"
                     /protein_id="HDL5120773.1"
                     /translation="MELNRRDFMKANAAVAAAAAAGIMIPVKNVQADDTGIRWDKAPC
                     RYCGTGCSVLVGTKDGKVVATQGDPDAEVNRGLNCIKGYFLSKMMYGADRMQEPMLRM
                     KDGKFDKNGDFTPVSWDQAFSIMAEKIKTTIKNDGPNAVGMFSSGQTTIFEGYAKVKL
                     WKGGFRSNTIDPNARHCMASAAVAFMRTFGMDEPMGCYNDIEKTDAFVLWGSNMAEMH
                     PILWSRISDRRLSDNKVKVVVMSTYEHRSFELADTPIIFKPHSDLAILNYIANYIIQN
                     DKVNWDFVNKHTKFKRGETNIGYGLRPEHKLQQNTNAKTAGKMYDSDFEEFKKIVAPY
                     TLEEAHRISGVPKDQLETLAKLYADPNQKLVSFWTMGFNQHTRGVWVNHMMYNVHLLT
                     GKIALEGCGPFSLTGQPSACGTAREVGTFIHRLPADMVVTNPKHREIVENSWKLPAGA
                     IPDVPGFHATAQSRALKDGKMKFLWQMCTNNMQGGPNINEEIFPGWRNPETFIVVSDP
                     YPSVSAVAADLILPTCMWVEKEGAYGNAERRTQFWRQQVKGPANARSDTWQLVEFSKY
                     FKVEEVWPEELLAKAPELRGKTLYEILYRNGKVDSYQVPTNIPGYMNDEADHFGYYIQ
                     KGLFEEYAEFGRGHGHDLADFDTYHKVRGLRWPVVKDEKTGEYKETLWRYREGYDPYV
                     KAGEEVAFYGNADKKAVILGVPYEPPAEAPDEEYDLWFCTGRVLEHWHTGTMTRRVPE
                     LHKAFPTNLVWMHPNDAKKRGLRHGDKVKVISRRGEYVSNLDTRGRNKCPEGLIYSTF
                     FDAGQLINKVTLDATDPISFETDFKKCAVKVVKA"
     gene            8025..8930
                     /gene="napG"
                     /locus_tag="PXA33_001450"
     CDS             8025..8930
                     /gene="napG"
                     /locus_tag="PXA33_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HDL5120774.1"
                     /translation="MKMDPNRRQFLKNATRTAAGVCGVGMILGLQQQQSFARQGVALR
                     PPGALPEQDFLSACTRCGQCVQACPYDMLHLASLISPVEAGTPYFIARDKPCEMCLDI
                     PCMNACPTGALSEELKDIDDARMGLAVLLDHETCLNWQGLRCDVCYRVCPLIDKAITL
                     EKVHNDRTSIHAKLIPTVHSDACTGCGKCEQACVLEEAAIKVLPMDLAKGLLGRHYRL
                     GWQEKQNAGKSLIEDVHPEGLRPAYDARMPEGLHEPVYQHMEVKPDVKVATPSRATQN
                     YVPNSTTVEAPEHFPDLDLNLKGVK"
     gene            8930..9808
                     /gene="napH"
                     /locus_tag="PXA33_001451"
     CDS             8930..9808
                     /gene="napH"
                     /locus_tag="PXA33_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HDL5120775.1"
                     /translation="MAANSPKNAGLEARQKLGWWHAYRFLILRRLSQLSIILMFLSGP
                     LWNVWILKGNYSGSLLLDTVPMTDPLMTAEVLATGYRPEWTVLVGALIMVAFYGIVGS
                     KLFCSWVCPMNMVTDTAAWLRRKLGIRQTAKIPRNLRYVILAVILVGSAISGTLLWEW
                     INPVAALGRVFVFGLGATLWLVLVVFLFDLLVAEHGWCGHLCPIGATYALIGAKSLIK
                     VKVVDRNRCDNCMDCYNVCPEPQVLRVPLHGKPEESTVILSKDCITCGRCIDVCAENV
                     FAFGMRFQGSIDVKNI"
     gene            9854..10282
                     /locus_tag="PXA33_001452"
     CDS             9854..10282
                     /locus_tag="PXA33_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HDL5120776.1"
                     /translation="MRKYLAFILAVMSGFAMAEAPKLSGSIDETTESVAPGFHNPQKD
                     VGTIPTTFPFQPPLVPHSIRGLQVSKNVNHCLGCHAIEPSKTTGATRLPPSHFTDRDG
                     KVYENESPRRYFCLQCHVQQTDVNPIIQNKFESVRESQAK"
     gene            10293..10901
                     /locus_tag="PXA33_001453"
     CDS             10293..10901
                     /locus_tag="PXA33_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HDL5120777.1"
                     /translation="MLRRFCNWLRSPSKMAIGAVILISALGGIFAWSGFNAGLAMTNT
                     EEFCSDCHMNDVVPEYRASAHYSNRSGVKAICSDCHVPHEFFPKWQRKIIAAKEVYAH
                     FTGKVDTKEKFEAHRGEMAEREWARMKANSSQECRNCHNFDDMDFTQQKNIAQQMHAL
                     AQEQGKTCIDCHKGIAHNLPHMEAIQKNFIPDDLLKKAEEKK"
     gene            complement(10963..11805)
                     /gene="cpdA"
                     /locus_tag="PXA33_001454"
     CDS             complement(10963..11805)
                     /gene="cpdA"
                     /locus_tag="PXA33_001454"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HDL5120778.1"
                     /translation="MDAKRMHEYIKLPVTGVIKLLQITDPHLFSNPEETLLNVKTVKS
                     FSAVIEQINKQAKQYFDLVLATGDLIQDNNIAGYHYFAQITNSLNSPIVWLEGNHDVQ
                     PSMSEILAQYKHILPHKQILLGEQWQLLMLNTQVVGMPYGELSYSQLKWLEEKLAQYP
                     QRFTLVALHHNILPTNSAWLDQHSLKNSNELSEIFAKFKNVKAIVHGHIHQQVDDIWH
                     GIPIYATPSTCIQFKPNCDQFTLDLQPQGWREIELHENGTIETVVKRLNSNDFLPNVN
                     AIGY"
BASE COUNT         3483 a         2372 c         2764 g         3305 t
ORIGIN      
        1 aagtgaaagg catttattgc tagataaaag cttccctttt caataaaaaa gagtatcata
       61 cgctctctta aacgtaaccc tacatttaag tctgtttcca acctatccca atttctttat
      121 tccttagggg catatagggt aaaaacaaaa aagctgaaac gcttttttta ataagtagcg
      181 agtcaaatgc cccatggcat ctattctttt aggaatagaa agcttaggct ttccgcaagg
      241 cacccgactt atttttttca cctcttgtag aaggtttatt ttcaatgaca attaaaactc
      301 ctattcaagc cgttcttgct tcaaaccaat attttttaga tcgccaagat gcaatggaat
      361 caaacgtgcg tagctaccca cgtaaattac cttttgctta ccaaaaagca caaggcgttt
      421 gggtaactga tgttgaaggc aatgaatacc tcgatttcct agcaggtgca ggaacgcttg
      481 cattaggtca taaccaccct aaattaatcc aagcagttaa agatgtatta gacagtggtt
      541 tacctttaca ctcgttagat attacgactc cgttaaaaga tgcttttacg gaagaattac
      601 tctctttctt tccaaaagat aaatatatcc tccaatttac tggcccgtct ggtgcagatg
      661 ctaacgaagc ggcaattaaa ttagccaaaa cctacactgg tcgtggcaac gtaattgctt
      721 tctccggtgg tttccacgga atgacccatg gtgcgttatc gctcaccggt aatttaagtg
      781 cgaaaaatgc agtacaaggc ttaatgccgg gcgtacagtt tatgccttat ccgcacgaat
      841 accgctgtcc gtttggcatt ggtggcgaag ccggtgcaaa agcagttgaa cattattttg
      901 agaactttat tgaagatgtt gaaagtggtg tggtaaaacc ggcagcggtg atccttgaag
      961 ccattcaagg tgaaggcggt gtggttccgg ctccaatcag cttcttacaa aaagtgcgtg
     1021 aagtcactca aaaacacggc atcttaatga ttgtggacga agttcaagct ggtttctgcc
     1081 gttcagggaa aatgtttgcc tttgaacacg caggcattga gccggatatc gtagtaatgt
     1141 caaaagcgat tggtggtagc ttaccgcttg cggtattagc gatcaaaaaa gagtttgacg
     1201 cttggcaacc ggcaggtcat accggtacat tccgtggtaa ccaattagca atggcaaccg
     1261 gctatgtatc gcttaaaatt atgcgtgaag agaatttagc gaaaaacgca caagagcgtg
     1321 gcgaatactt aactaacgca ttacgtgaat taagcaaaga attaccttgt attggcaatg
     1381 tgcgtggtaa aggcttaatg atgggaattg atattgttga tgaacgtcag ccaaaagatg
     1441 caacaggtgc atacccgcgt gattgcgatt tagccgttgc aattcaaaaa tactgcttta
     1501 aaaacaaatt gttattagag cgtggcggac gtggcggtaa tgtggtgcgt gttctttgtg
     1561 ccgtgaatat tacccaagca gaatgtgaag agtttattaa acgctttaaa caatctgttg
     1621 ctgaagcagt gaaagcggtg cgtggttaat agttagaatg gtaggagcga aacatttttc
     1681 gccctaatgt aaaaaggggc gaatatttat tcgcccctac accaatagga tagaaataat
     1741 gtcaaaccta gaaaaacaca gacaatccct gttttgtagc gatccaaaat cgattgcgga
     1801 ttatgaatcg gcaatgaata acgctgtgaa agcggtttca gactggctga aagaagaaaa
     1861 aatgtacacc ggtggctcaa ttaaagagtt gcgtgccaaa atcaacggtt ttaacccaac
     1921 ccaagctggt gtgggcgtgc aaaaatcact ggagcatttg gtggaaattt tcttaaagcc
     1981 aagcctcaaa gttcaccacc ctcattcttt ggcccactta cattgcccga caatggtaac
     2041 cagccaaatt gcagaagtgt taattaatgc caccaaccaa tcaatggact cttgggatca
     2101 aagcccggca ggttcgatta tggaagagca tttaattgac tggctacgtc aaaaagtcgg
     2161 ctatggcaaa ggaacttcag gagtctttac ctcaggtggt acgcaatcaa acctgatggg
     2221 cgtattattg gctcgtgact gggcgatagc gaaccattgg aaaaaggcag acggttcaga
     2281 gtggtcggtg caagccgacg gtattcctgc cgatgcaatg caaaaagtga aagtggtttg
     2341 ttccgaaaac gctcacttct cggtgcagaa aaatatggca atgatgggaa tgggtttcca
     2401 atcggtagtg actgtaccgt caaatgccaa tgcacaaatg gacgttgaag ctcttgcgaa
     2461 aacgttggcg gatttaaaag ccgaaggcaa aattgtggct tgtatcgtcg caaccgcagg
     2521 gacgaccgat gccggtgcga ttgatgattt aaaagcgatc cgtaaattag ccgatgaaca
     2581 tcaggcttgg gttcacgttg atgctgcttg gggtggtgca ttattactgt cgaaagattt
     2641 ccgccacttc ttagacggca ttgaattaac cgattccatt acccttgatt tccacaagca
     2701 tttcttccaa tcaatttcgt gtggggcatt cttgctaaaa gatgaagcga attaccgctt
     2761 tattgattac aaggcagact acctgaattc agaatatgac gaagaacacg gcgtgccgaa
     2821 tttagtgtca aaatcattgc aaactacccg ccgttttgat gcgttaaaat tatggtttac
     2881 cgttgaagcc ttgggtgagg agctatacgc ttcaatgatc gaccacgggg tttatctttc
     2941 taaagaagtg gaagcctaca ttaatgccac cgatggcttg gaaatgttag tgccggcaca
     3001 atttgcctca gtcttattcc gtgtcgtacc acaaggttac ccggcagaat ttgtggatac
     3061 cttaaaccaa aatgtggcag atgagttatt tgctcgtggc gaagctaaca ttggtgtgac
     3121 caaagtcggt gagcatcaat cactgaaaat gactacttta agcccgattg caacgcttga
     3181 aaacgtaaga gcattattag ctcaggtgtt ggcagaagca gatcggatta aagattcgat
     3241 tgtaaatgga acttatgttc cgccaattga ttaaactgcg ttaaaaataa aaaggtgttt
     3301 tcgacattta tttgtgtttt aatgtttgca ttcggtagtc tttatcgaat gcaaattttt
     3361 ttaacaagga gtttttatga aaaaagtcct gggtttaaca ttgatggcaa tggcgttatc
     3421 ggcgtgtaat tcaactgctt ccaacggttc ttcacaagca aatacaggtg ttgcaccggc
     3481 gaatgtgatt agcgtgaaac aggcgttagc ggcagcagat gacagctacg tgacggtaga
     3541 aggcacactc ttaaacaaag tcggtgacga aacttactta tttaaagatg cgacagggca
     3601 aatccgtgtt gagattgacg atgaagtatg ggcaggtcag cacgttggta gcgaaggcaa
     3661 taaagtccgt ttatacggag agattgacaa aggtttcaat aaaaccgagt tagatgttaa
     3721 aacactcact gttctgtaga agatcaaacc ttaatttggt tccccgcctt gtttggcggg
     3781 gtttttacgt taattaatca aaccatcctt ttgcaaaaaa acgcaaaatt ataccgcttg
     3841 ttccttcaat attgtgctac aagatgctgt gatttgctgc aaaatagcgg tgtaatcagt
     3901 ctcttttaaa ccaatattac cgaaatagtg catttttaca tcacgaatcc cacagaactc
     3961 aaacagccct tttcccagcg tatgttcaaa tgctgctaaa aagccttttc gttcatattt
     4021 ttcattggaa ttaccgagcg ttacaaattg ttgcattttt ttgccggtca gcaagccgat
     4081 actttcaatt tccccgttac gataggcaaa atcataactt aacaccctgt ctaaatagcc
     4141 ttttaaaatt gccggaaagc ccatccacca aagcgggtaa attaacgtaa tcacatcagc
     4201 ttcaagccaa tattgatgct caattgccac atctgcttca tagtgtttac tgagtgtgcc
     4261 ttgaaattcc gcccagtcta atatcggctt gaagttgagt tggtagagat ctcgtacaac
     4321 caccttattt ggaacggaag ctgctatcgt tttttccaaa atcgcatggt taaagctgga
     4381 gggattcggg tgtgcgtaaa taatcaggtg attcatagcg ttttccttta ctgtgtcgag
     4441 gcagctacta gaagatttta gcgaaacttt ctttgatgaa aatcaatatt ttggcgaata
     4501 taccggcaaa taccccgttt gaggaatgaa cggttttaag tgaacattag ataatttagc
     4561 ctattatagt gatgagtaaa aaggtgtttt cttgactgat tatcctaatc aatcacttcc
     4621 tcgccgtagc tttttgcgag ggcaattttt agactcgtta aaaagccaaa aagtcagtca
     4681 gcaagggtat gagccgattc gtccgccttg ggctaattta gcaaattttt tagaaaaatg
     4741 caccgcttgc ggtaactgca ttcaggcttg cgaaacccag attattgtaa aaggtgctgc
     4801 gggctaccct gaaattgatt ttagccgtgg tgaatgcact ttttgtgaaa aatgtgtgca
     4861 ttcttgcgag gcagaggtat tccgagcagt ttcagaacag ccttggagcc ataaaattga
     4921 gattcaagcc ggctgtttat taaagctcgg aacggaatgc cgaagctgtg gcgatagctg
     4981 tgagatgaga gtgattcgtt tccgcccgag tttaggtggc attgccgaaa tggtattaaa
     5041 tttggaaagc tgtaatggct gtggggcttg tttgagcgtt tgtccgacaa gtgcgataaa
     5101 aattcagcca ttacaaacac aggcaaacag agatgagtga aaaattatcc gaacaggaaa
     5161 actggtatgt gtgcagtctc gtggtgcaag caaaaccgga aaaattggaa gaagtaaaag
     5221 ccgagattct gaagattcct cataccgaaa ttcacggtga gaaaacagat gaaggcaaat
     5281 tggtcgtgac gattgaaagc aatgtgcatt tagccctttc cgatcgaatc gatcaagtaa
     5341 gagatattaa aggcgtgatt gtggtgtctt taatttctaa ttacattgat gaacagtgat
     5401 taatgattat ttttgaagcc aaacgtggga catagttatg gaactcaatc gtagagattt
     5461 tatgaaagcg aacgccgctg tggcagctgc tgcagccgca ggaattatga ttcctgtgaa
     5521 aaacgttcag gcagatgaca ccggtattcg ttgggataaa gccccttgtc gttactgtgg
     5581 tacaggttgt agcgtgctag tcggtactaa agacggtaaa gtagtggcaa cccaaggtga
     5641 cccggatgca gaagtaaacc gtggtttaaa ctgtattaaa ggttatttct tatccaaaat
     5701 gatgtacggt gcagaccgta tgcaagagcc aatgttgcgt atgaaagacg gtaagttcga
     5761 caagaacggc gatttcacgc cggtttcttg ggatcaagcc tttagcatta tggcggagaa
     5821 gattaaaacc actattaaaa atgacggccc gaatgctgta ggtatgttct catcaggtca
     5881 aacaaccatt tttgaaggtt atgcgaaagt gaaactttgg aaaggtggtt tccgttcaaa
     5941 tacgattgac ccgaatgccc gccactgtat ggcgtccgct gcggttgcct ttatgcgtac
     6001 ctttggtatg gacgagccga tgggctgcta taacgacatc gaaaaaaccg atgcgttcgt
     6061 gctttggggt tcaaatatgg cagaaatgca cccgatttta tggtctcgta tttctgatcg
     6121 ccgtttatca gacaataaag tgaaagtggt ggtaatgtcc acttacgaac accgttcgtt
     6181 tgaattagcc gatacaccga ttatcttcaa accacactca gatttagcaa ttttaaacta
     6241 cattgcgaac tacattatcc aaaacgataa agtaaattgg gattttgtta acaaacacac
     6301 caaatttaaa cggggtgaaa ccaatatcgg ttacggttta cgtcctgaac acaaattaca
     6361 acaaaatacc aatgcgaaaa ctgccggtaa aatgtatgac agcgattttg aagagtttaa
     6421 gaaaatcgtt gccccttata cattagaaga agctcaccgt atttcaggcg tgccgaaaga
     6481 tcagcttgaa acattagcga aattatatgc agatcctaac caaaaattag tgtcgttctg
     6541 gacgatgggc tttaaccaac atacccgtgg tgtgtgggta aaccatatga tgtacaacgt
     6601 ccatttatta accggtaaga tcgcattaga aggttgtggg ccgttctcac tcacgggtca
     6661 gccgtccgct tgtggtactg cccgtgaagt gggtacattt attcaccgct taccggcaga
     6721 tatggtggtc acaaatccga aacaccgtga gattgtagag aattcttgga aattaccggc
     6781 tggagcaatt cctgatgtac cgggctttca cgctacagct caaagccgtg ccttaaaaga
     6841 cggcaaaatg aaattcttat ggcagatgtg taccaacaat atgcagggcg gcccgaatat
     6901 caatgaagag atcttcccgg gctggcgtaa tcctgaaacc tttattgtgg tttcagatcc
     6961 gtacccatct gtttcagcag tggctgctga cttaattctt ccaacctgta tgtgggtgga
     7021 aaaagaaggg gcttacggta acgcagaacg ccgtacccaa ttctggcgtc agcaagttaa
     7081 agggccggca aatgcccgtt cagatacttg gcagttagta gagttctcta aatacttcaa
     7141 agtagaagaa gtatggccgg aagagttatt agctaaagca ccggaattgc gtggtaaaac
     7201 tctatacgaa atcctttacc gcaacggcaa agtagatagc taccaagtgc caactaacat
     7261 tccgggctat atgaatgacg aagcagatca cttcggttac tacattcaaa aaggcttatt
     7321 tgaagagtat gcggaatttg gccgtggtca cggtcacgat ttagccgact ttgacaccta
     7381 ccacaaagta cgtggcttac gttggccggt ggtaaaagac gagaaaaccg gcgaatacaa
     7441 agaaacttta tggcgttacc gtgaaggcta tgacccatac gttaaagccg gtgaagaagt
     7501 ggcattctat ggcaatgcgg acaaaaaagc agtgatttta ggtgtgccat acgaaccacc
     7561 tgcggaagca ccggacgaag aatatgactt atggttctgt accggtcgtg tactagaaca
     7621 ttggcacacc ggtacgatga cccgccgtgt gccggagtta cataaagcat tcccaaccaa
     7681 cttggtttgg atgcacccga atgatgcgaa aaaacgaggc ttacgtcacg gtgataaagt
     7741 gaaagtgatt tcacgccgtg gtgaatatgt atcgaactta gatacccgtg gacgtaataa
     7801 atgtccggaa ggtttgattt actcgacttt cttcgatgcc ggtcagttaa tcaacaaagt
     7861 gacacttgat gcgactgatc caatttcatt tgaaaccgac ttcaaaaaat gtgcggttaa
     7921 agtggtgaaa gcttaattgt aggggcaaat tatttgcctc agacaagcgg tcaaatttgc
     7981 aaattttttt acaaaaacga ccgcttacct aagaggaacc cacaatgaaa atggatccaa
     8041 atcgtcgcca gtttttaaaa aatgcaaccc gtaccgcagc cggtgtttgc ggtgtaggaa
     8101 tgattttagg gctacagcaa caacaaagtt ttgcccgtca aggggtagca ctacgcccac
     8161 cgggtgcatt gccggagcaa gatttcctga gtgcttgcac ccgttgcgga caatgtgtgc
     8221 aggcttgtcc ttacgatatg ttgcatttgg catcgcttat ttcaccagtg gaagccggca
     8281 cgccatactt tattgcacgg gataaacctt gcgaaatgtg cttggatatt ccctgtatga
     8341 atgcttgccc aaccggggca ttaagcgaag agctaaaaga cattgacgat gcacgaatgg
     8401 ggcttgcggt gttgctcgac cacgaaactt gtcttaactg gcaaggcttg cgttgtgacg
     8461 tgtgttaccg tgtttgtccg ttaatcgaca aagcgattac tcttgagaaa gtgcataacg
     8521 accgtacctc gattcacgcc aaactgattc cgaccgtgca ttctgatgct tgcaccggtt
     8581 gcggtaaatg tgaacaggct tgcgtattgg aagaggcagc gatcaaagtg ttaccgatgg
     8641 atttagccaa aggcttgctc ggtcgtcact atcgtttagg ctggcaagag aaacaaaatg
     8701 ccggtaaatc gctgattgaa gatgttcacc cagagggttt acgcccagct tatgatgcca
     8761 gaatgccgga aggcttacac gagccggtgt atcagcatat ggaagtaaaa cctgatgtga
     8821 aagtggctac gccaagtcgg gcgacacaaa actatgtgcc gaactctacg acggtggaag
     8881 ctccggagca tttccctgat ttagacttga atctcaaggg ggtgaaataa tggcagccaa
     8941 ttctccaaaa aatgcgggct tagaggctcg tcagaaacta ggttggtggc acgcttaccg
     9001 ctttttgatt ttaagacgct taagccaact cagcattatt ttgatgtttt taagcggccc
     9061 actttggaat gtctggattt taaaaggaaa ctacagcggt agcttattac tagataccgt
     9121 gccgatgaca gacccattaa tgaccgctga agtgctggca accggctatc gccctgaatg
     9181 gacggtattg gttggggcgt taattatggt ggcgttttac ggcattgtcg gcagcaagct
     9241 gttttgtagt tgggtctgcc cgatgaatat ggtaacagat actgctgctt ggttaagacg
     9301 caaactcggt attcgccaaa ctgctaaaat tccacgcaat ttacgctatg tgattttggc
     9361 ggtgatttta gtcggtagtg cgatttccgg aacactattg tgggagtgga tcaacccggt
     9421 tgcggctctc ggtcgggtgt ttgtattcgg tttaggggca acgctttggc tggttttagt
     9481 ggtgttcttg tttgacttat tggtggcaga acatggctgg tgtggtcatc tttgcccgat
     9541 tggtgcaacg tatgccttaa ttggggcgaa aagcctgatt aaagtcaaag tggtagatcg
     9601 taaccgttgc gataattgta tggattgtta caacgtttgt ccggaacctc aagtgttgcg
     9661 tgtgccgcta cacggtaaac cggaggaaag cacggttatc ttatctaaag attgcattac
     9721 ctgcggacgt tgtattgacg tttgtgccga aaatgtattt gccttcggaa tgcgttttca
     9781 aggcagtatt gatgtgaaaa atatttaatt tttatctttt tgaaaacaag aattttaatg
     9841 gagtgaacct caaatgagaa aatatctcgc cttcatttta gccgtgatgt ccggctttgc
     9901 aatggcggaa gcccctaagt tatccggatc aattgatgag acaaccgaaa gtgttgcacc
     9961 gggatttcat aacccacaaa aagatgtggg cacgattcca accactttcc ctttccaacc
    10021 accgcttgtt ccacatagta ttcgtggctt acaagtgagc aaaaacgtaa accattgttt
    10081 aggttgccac gctattgaac catctaaaac cacaggtgcg acccgtttac cgccaagcca
    10141 ttttaccgac cgtgatggca aggtgtacga aaacgaatct cctcgtcgtt atttctgttt
    10201 gcaatgccac gtgcaacaaa ctgatgtaaa cccgattatt caaaataaat ttgaatctgt
    10261 acgtgaatct caagctaaat aaggagtaag taatgttgag aagattttgt aactggttac
    10321 gtagcccaag caaaatggca attggtgctg taatccttat ttctgcatta ggtggtattt
    10381 tcgcttggag tggttttaat gcagggttag caatgacaaa tactgaagag ttttgttcag
    10441 attgccatat gaacgatgtt gtgccggaat accgtgcctc tgctcactat tcaaaccgca
    10501 gcggtgtgaa agcgatttgt tcagattgcc acgtaccgca cgaattcttt ccaaaatggc
    10561 aacgtaaaat catagccgca aaagaagttt atgcacactt taccggtaaa gtagatacca
    10621 aagagaaatt tgaagctcat cgtggcgaaa tggcagaacg tgaatgggca cgtatgaaag
    10681 cgaacagctc tcaagagtgc cgtaactgtc ataattttga tgatatggac ttcactcaac
    10741 aaaaaaacat tgcgcagcaa atgcacgcat tggcacaaga gcaaggtaaa acctgtattg
    10801 actgccacaa agggattgca cacaatctcc cacatatgga agcgatccag aaaaacttta
    10861 tccctgatga tttgctgaaa aaagcagaag agaagaaata acttaacgtg tttcaataga
    10921 aaaccctcaa tttccattga gggtttcttt ttagtagcca agttagtaac caatggcatt
    10981 gacatttggt aaaaaatcgt tggaatttaa ccgcttgacg accgtttcta tcgtgccgtt
    11041 ttcgtgcaat tcaatttcac gccagccttg tggttgtaag tctaaggtaa actggtcgca
    11101 attcggctta aattgaatgc aagtagatgg ggtggcataa atcggaatac catgccaaat
    11161 atcatcaacc tgctgatgaa tatgaccatg cacaatcgct tttacattct taaattttgc
    11221 gaaaatctcg gataattcat tgctgttttt taagctatgc tgatccagcc acgctgagtt
    11281 tgtcggcaaa atattgtggt gcagagcaac caaggtaaac cgttgcggat attgtgccaa
    11341 tttttcttct aaccatttaa gctgtgagta agagagttcg ccataaggca tacctaccac
    11401 ctgggtattt agcatcagaa gctgccattg ctcaccgagt aaaatttgtt tatggggaag
    11461 gatatgttta tattgcgcca aaatttctga catactcggc tgaacatcgt gattcccctc
    11521 tagccacact atcggcgagt ttagcgaatt agttatctgt gcaaaatagt gataaccagc
    11581 aatattgtta tcttgaatca aatcacctgt ggcaagaact agatcaaaat attgcttcgc
    11641 ttgtttgttt atttgctcaa taactgccga aaaacttttt actgttttga cattaagtag
    11701 agtttcctcc ggattggaga aaaggtgagg gtcagttatt tgtaataact taattacccc
    11761 tgttacgggt aacttaatat attcgtgcat tcgttttgca tccattcaaa ttctagtaat
    11821 ttttaactgg tgagattcta tcagtaagct aaaagtaaaa atgtggacta tgccgcattt
    11881 ttgaaaaaaa gtgagattaa tcaaaaaaaa tttattattt tcga
//
LOCUS       DANLRK010000076        11777 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.076, whole genome shotgun sequence.
ACCESSION   DANLRK010000076 DANLRK010000000
VERSION     DANLRK010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 11777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11777
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.076"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            108..2000
                     /gene="mnmG"
                     /locus_tag="PXA33_001455"
     CDS             108..2000
                     /gene="mnmG"
                     /locus_tag="PXA33_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HDL5120779.1"
                     /translation="MIYNQIYDVIVVGGGHAGTEAALAPARMGLKTLILTHNVDTLGQ
                     MSCNPAIGGIGKGHLVKEIDAMGGLMAKATDVAGIQFRTLNNSKGPAVRATRAQADRV
                     LYRNAVRTALENQPNLDIFQQEVVDILVENNRAVGAVTKMGLTFKARAVVLTAGTFLA
                     GKIHIGLDNYAGGRAGDPAATMLADRLRDLNLRVDRLKTGTPPRLDARTINFDVLAKQ
                     HGDEVLPVFSFMGDVSQHPRQIPCYITHTNDQTHDLIRNSLDRSPMYTGIIEGVGPRY
                     CPSIEDKVMRFADRNSHQIYLEPEGLNTIEVYPNGISTSLPFDVQMGIVNSMKGLENT
                     RIIKPGYAIEYDYFDPRDLKPTLETKAIDGLFFAGQINGTTGYEEAGAQGLLAGINAA
                     LQVQGKEAWYPTRDQAYTGVLVDDLCTLGTKEPYRVFTSRAEYRLLLREDNADIRLTP
                     KAHELGLIDEARWVRFNQKMEAIEQERARLKSTWAHLQMPNLDELNALLANPLAREAS
                     GEDLIRRPEMSYEKLTQIALFAPAIEDKQVAEQVEIAIKYQGYIEHQYNEIERHKRHE
                     NTLIPVEFDYDKVESLSNEVRAKLMQHRPVSIGQASRISGITPAAISILLVNLKKQGM
                     LKRGEL"
     gene            2095..2319
                     /locus_tag="PXA33_001456"
     CDS             2095..2319
                     /locus_tag="PXA33_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5120780.1"
                     /translation="MIYLRKLGFSPFDHQMFAQPSWYFCNALVHTNYENLRQGVFVTK
                     AYLVMFLENLLFEKSHSLSNRALRINENND"
     gene            2332..2946
                     /gene="rsmG"
                     /locus_tag="PXA33_001457"
     CDS             2332..2946
                     /gene="rsmG"
                     /locus_tag="PXA33_001457"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HDL5120781.1"
                     /translation="MLAKLNRLLAQAEINLTDQQKEQLVGFVRLLDKWNKAYNLTSVR
                     NPDEMLVKHILDSLVVSRHLQGETFIDVGTGPGLPGIPLAIANPDKQFVLLDSLGKRI
                     TFIKNALRELGIKNVTPVQSRVEEYTESQFDGVLSRAFASLNDMIDWCYHLPTENGKF
                     YALKGVYDEQEIIEIKQPIQLVEVIKLEVPELVGERHLVVLKKP"
     gene            3214..4662
                     /locus_tag="PXA33_001458"
     CDS             3214..4662
                     /locus_tag="PXA33_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL5120782.1"
                     /translation="MNRVTNKADEFAKLSTFERIAYGSGDLAQNLVFGTVGGFLLTYL
                     TTVNGISAVAGATIFLIVKWGNVFWDPFVGAFIDKSKPSAQGKYRPYLLKFGIPLVVL
                     ASLLFLPLESVRGSVIYAFLSYFATAMVYSFVNIPYGSLSASLTRDTDEIAKLTTTRM
                     TMANIANLFVYTLFPLFVQLLAPHAELKNTGFFGIELYLGDYMSAAAGPAWFKVYAVY
                     MVIGFVALMLSYFGTKERIIPTAEETDKVKYSDLVNELKRNRPLQILGLFFLIGFTFM
                     FFGNTVWPFYLKYNIGHSEWIASVGLLGSIPGIFLVFLWPKLRRTIGKKNFFYLFLGI
                     FVLGQLCLWVWQFDSFKYSPTLGYIGRFLQQWGLTSATGFMWALVPEVVTFGEFKSKK
                     RVAGIINALMGLFFKIGLALGGIIPGYINAVYGFNGSLLAQTEEALFGINLSMIWAPI
                     ILAIIAGFVMSRYPLSDTDIDHINEKITAYSK"
     gene            4685..5731
                     /locus_tag="PXA33_001459"
     CDS             4685..5731
                     /locus_tag="PXA33_001459"
                     /inference="COORDINATES: protein motif:HMM:NF016497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HDL5120783.1"
                     /translation="MQIQNPVLPGFNPDPSIVRVGDTYYIATSTFEWFPGVRIHESKD
                     LVHWNLVKNVLDTTTLLDMRGNPSSGGIWAPDLSYAEGKFWLVYTDVKITDGAFKDMT
                     NYLTTAKEIQGPWSAPIKLNGIGFDASLFHDDDGRKYLVQQTWDHREYHHPFDGITLT
                     EFDVATMKLKPETQRTIFEGTEVKLVEGPHIYKINGEYYLFAAEGGTVFTHQEVVARS
                     KTLEAHSFQLAPNTPFITNFDTPNSYLQKQGHGSLVDTPSGEWYYASLTARPWNHATE
                     SATDPRGWSTLGREAAIQKVEFDQDGWPYIVGGHGGKTFVEAPKDAIFTEAPIDHSQH
                     DEFDSSTLDINWNT"
     gene            5734..6372
                     /locus_tag="PXA33_001460"
     CDS             5734..6372
                     /locus_tag="PXA33_001460"
                     /inference="COORDINATES: protein motif:HMM:NF036826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,4-xylosidase"
                     /protein_id="HDL5120784.1"
                     /translation="MPFSKEMGEVGNGVLKLIGRGSLANTHDLSLIARRWQAFYFDAE
                     TKVKFTPFSYQSMAGLTNYYNHSHWSWIFITKNDQGQQVIEVAENKGGLRNGQYTSYL
                     KDKAIVIPDGTEYVWFKTKVRKETYRYEYSFDGKQWHTIPVELDAAVLSDDYVLRSYG
                     GFFTGAFVGLAAVDYSSYESTAEFSYFDYKELGDKQLTDGSFSWEKSESCFG"
     gene            6391..7371
                     /locus_tag="PXA33_001461"
     CDS             6391..7371
                     /locus_tag="PXA33_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDL5120785.1"
                     /translation="MKQRRFGKTNKMVSEIGLGTWQLGTKWGEPFNHQEAMAILETAY
                     EQGINFIDTADVYNNGQSEKAIGDILKKYPDFFYVVTKCGRALNPHTAEMYTPQAIEK
                     FVDGSLSHLGTEKLDMILLHCPPTLVYRNDEIFTKLEQLKAGGKLIDYGVSIETAEEG
                     LLAMEYDIAAMEVIFNMFRLKPLEQLFPTAQQKNVGIIARVLLASGLLTGRYNACTQF
                     GKDDHRTFNRNGEAFDKGETFSGVDYQLGLQAVEELKALFGTEDLIPYALRWVLMNEA
                     VSTVIPGASKVSQVITNAAVDNFPPLTENEMQKVEDIYNRLIRPSVHHLW"
     gene            complement(7433..8773)
                     /locus_tag="PXA33_001462"
     CDS             complement(7433..8773)
                     /locus_tag="PXA33_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL5120786.1"
                     /translation="MFSFLKASPPAEQKVEASRVDAEYRKLRWQVFAGVFIGYAAYYL
                     IRKNFSLAMPYLIDEYGFTKADLGTVGVALSLAYGFSKFIMGNVSDRSNPKYFITIGL
                     LGSAIVSLVFGLVPGVLSSIPIMIILAALNGWFQGMGYPPGAKTMTNWFSTSERGVWW
                     SWWNVSHNLGGGLIGPLAILGLSIFGVWQSLFYLPALIAIVLAFVMFYLMRDTPESQG
                     LPPIDEYKGEKVVEKVESAHTLSSKDIFYKYILNNKFLWAIAIANVFVYFIRYGIIDW
                     APTYLKEVKHFSVDKQSWAYFLYEYAGIFGMLASGYLSDKVFKGHRAPPMLLFLAGVL
                     IAIVVYWKNPAGNPLIDNICLVAIGFLIYGPVMMIGLQAADLVPRVATGTATGLTGLF
                     GYLLGSASAGYVMGKLVDMFGWDGGFYALILSCFLAFAFIALTLFKKTSKQLEV"
     gene            complement(8963..10036)
                     /gene="glpQ"
                     /locus_tag="PXA33_001463"
     CDS             complement(8963..10036)
                     /gene="glpQ"
                     /locus_tag="PXA33_001463"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDL5120787.1"
                     /translation="MKFKFKTLVAALALSATALGAYAQQSDKLVIAHRGASGYLPEHT
                     LESKALAFGQQADYLEQDLAMTKDNRLIVIHDHFLDGLTDVAKKFPERKRADGRYYVV
                     DFTLDEIKTLEMTENFEVKDGKQAQVYPNRFPMWQSHFTIHTLEEELEFIQGLEKSTG
                     KKIGIYPEIKAPWLHHKEGKDIALETLKVLKKYGYDSKTSPIYLQTFDFNELKRIKTE
                     LLPKLGMDVKLVQLVAYTDWHETEEKDKNGNWVNYDYDWMFKDGAMAEVAKYADGVGP
                     GWYMLVDKEKSKVGNIVYTPLVKELAKYKMELHPYTVRKDALPDFFQNVDEMYDALLN
                     KAGATGVFTDFPDTGVEFLKKQK"
     gene            complement(10106..11551)
                     /gene="glpT"
                     /locus_tag="PXA33_001464"
     CDS             complement(10106..11551)
                     /gene="glpT"
                     /locus_tag="PXA33_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HDL5120788.1"
                     /translation="MFGPFKPAPHIAELPADKVDARYKFLRWQVFAGIFFGYAAYYFV
                     RANFDLAQKGLIEAGMYTKTDLGVIGTAAGLAYGLSKFVMAGMSDRSNPKVFLPTGLL
                     LSGLCMTLMGLMPWATSSILVMWVMIFLNGWFQGMGWPPCGRTMVHWWSKSERGTIVS
                     IWNTAHNLGGMVPGAMVLLASALYFSEYGVEATAKDVWQQALYFPGIAAMIAAIPIYL
                     VMKDTPQSCGLPPVEKWRNDYPDDYNEKTYEHDLSTKEIFVTYVLKNKLLWYIAIANV
                     FVYLIRYGVLKWSPVYLGEVKHFNIKGTAWAYTIYELAAIPGTLLCGWVSDKLFKGKR
                     GLTGFIFMILTTAAVVAFWLNPATPESEIAQYAGHAWYENPYQLMDFIWMTTIGFLIY
                     GPVMLIGLHALELAPKKAAGTAAGFTGLFGYLGGTVAASAVVGWAADKFGWDGGFYVM
                     IGGGVLAILLTFIVMVEEGKHKAKLEDHYGK"
BASE COUNT         3418 a         2472 c         2513 g         3374 t
ORIGIN      
        1 cgcatttaga tcttaacctc tctaaaaatc tatttcccag atgatccctt tttacgctaa
       61 aatatgcgtt catttttcag cagaatttgt aagatagtag aagagatatg atttacaacc
      121 aaatttacga tgtgatcgtg gtcggcggtg gtcacgccgg cacagaagcg gccctagcac
      181 cggcacgaat gggcttaaaa accctgattt taacccacaa tgttgatacc ttagggcaaa
      241 tgtcgtgcaa cccggctatc ggagggatcg gcaaaggcca tctggttaaa gagatcgatg
      301 cgatgggcgg tttaatggca aaagcaacag acgttgccgg gatccaattc cgtaccctaa
      361 acaactcaaa agggcctgcg gtgcgtgcca cccgtgccca agctgaccgt gtgctttacc
      421 gtaatgcggt acgcactgcg ttagaaaatc aaccaaattt agatattttc cagcaagaag
      481 tagtggatat tttggtggaa aacaaccgtg cagtgggtgc agttaccaaa atgggcttaa
      541 cgtttaaagc ccgtgcggta gttttaaccg ctggtacgtt cttagcgggt aagatccaca
      601 ttgggttgga taactatgca ggtggtcgtg ccggcgatcc tgctgcaaca atgttggcag
      661 atcgtctgcg tgatctgaat ttgcgtgttg atcgccttaa aacaggtaca ccacctcgcc
      721 tcgatgcccg taccatcaac tttgacgtac ttgccaaaca gcacggcgat gaggtgttac
      781 ctgtcttctc ttttatgggc gatgtctcac agcacccacg ccaaattccg tgctacatca
      841 cccacactaa cgatcaaacc cacgatttaa ttcgcaacag tcttgatcgc agcccgatgt
      901 acaccggtat tatcgaaggg gttggtccac gttactgccc gtctattgaa gataaagtaa
      961 tgcgttttgc cgatcgtaat agccaccaga tctaccttga accggaaggc ttaaatacga
     1021 ttgaggttta cccgaacggg atctccacca gcttgccgtt tgatgtgcaa atggggatcg
     1081 tcaactcaat gaaaggcttg gaaaacaccc gcatcatcaa accgggctat gcgattgagt
     1141 acgactactt cgacccgcgt gatttaaaac caacccttga aaccaaagcg attgacggtt
     1201 tattctttgc cggacaaatt aacggcacga ccggttacga agaggcaggt gcgcaaggtt
     1261 tgctggcagg gattaacgca gcgttacagg ttcaaggtaa agaggcgtgg tatccaaccc
     1321 gtgatcaagc ctacaccggc gtgttggtgg acgacctctg tacattaggc accaaagagc
     1381 cataccgtgt gtttacctct cgtgccgaat accgtctgtt gctgcgtgaa gacaatgcgg
     1441 acattcgttt aacaccaaaa gcccacgaac taggattaat tgacgaggca cgttgggtgc
     1501 gtttcaacca aaaaatggaa gcgattgagc aagaaagagc tcgtttgaaa tccacttggg
     1561 cacatttgca aatgccaaat ctggacgaat tgaacgcgtt attggctaat cctttagccc
     1621 gtgaagcaag cggtgaagac ttaatccgcc gtcctgaaat gagttacgaa aagctgaccc
     1681 aaatcgccct atttgcccct gctattgaag acaagcaagt ggcagaacag gtggaaattg
     1741 cgatcaaata tcagggctat attgagcatc aatacaacga aatcgaacgc cataaacgcc
     1801 acgaaaacac cctgattccg gtagagtttg actacgacaa agtcgaaagc ctctccaacg
     1861 aagtgcgagc taaattgatg caacaccgcc ctgtctctat cgggcaggca agccgcattt
     1921 ccggcatcac accggcagcg atttccattc tgttggtgaa tttgaagaag caaggaatgt
     1981 tgaaacgggg tgagctgtaa atactacaag cggttggttt tgcagaaatt tttgcaaaat
     2041 tgaccgcttg tattctttgg agaaggaaat acccgaacta ccgccgtatt tttaatgata
     2101 tatctgcgta aactcggttt ttcgccgttc gatcaccaaa tgtttgccca accttcgtgg
     2161 tatttctgca atgcacttgt tcataccaat tatgaaaatc tgcgacaagg tgtatttgtg
     2221 actaaagcgt atttagtgat gtttcttgaa aatttattgt ttgaaaaaag tcattcgtta
     2281 agtaatcggg cattgcgtat taatgaaaat aatgattaag gaatttgcaa aatgttagct
     2341 aaattaaacc gcttgcttgc tcaagccgaa ataaatttga ccgatcaaca aaaagagcag
     2401 ttagttggtt ttgtgagatt attggataaa tggaataagg cgtataacct cacttccgtt
     2461 cgcaaccctg atgaaatgtt ggttaaacat attttggaca gccttgttgt tagtcgtcat
     2521 ttacaaggcg aaacgtttat tgatgtcggc acggggccgg ggctaccggg cattccgctg
     2581 gcgattgcta accctgataa acagttcgtg ctactagaca gcttaggcaa acgcatcact
     2641 tttatcaaaa atgcgttgcg tgagcttggt attaaaaacg ttaccccagt gcaaagtcga
     2701 gtggaggaat acactgaaag ccagtttgac ggcgtattaa gccgagcctt tgcctcgctt
     2761 aatgatatga tcgactggtg ttatcattta ccgactgaaa acggtaaatt ttatgcatta
     2821 aaaggcgtgt atgatgagca ggaaataatt gaaatcaaac aaccgattca gctagtggaa
     2881 gtgattaagc ttgaagtacc tgagttggta ggagaacggc atttagtagt gttgaaaaag
     2941 ccctaataca ttggaagttt aatgatacaa gcggtcgaat ttgtaaattt ttttgcaaat
     3001 ctgcctgctt gtgttaacta tttttcataa agcatacctg gttaataagg tgtgcttttt
     3061 ttataaccta ttatttcatc tgatttattt ctattttgtg atgtgcttca caaaaacaaa
     3121 aaaacataat gtagtaaaaa atattcgtaa ttgaagccct tatttcttta cctgaaaata
     3181 agcgaactta attatataac gaggaggttc gtaatgaaca gggtaacaaa taaggcagat
     3241 gagtttgcta aactcagtac gtttgaacgt attgcttatg gtagtggcga cttagcccaa
     3301 aatttagttt ttggtacagt tggtggtttc ttactcactt acttaactac tgtaaacggc
     3361 atttctgccg tagcaggagc aacgattttc ttaattgtaa aatggggaaa tgtgttttgg
     3421 gatccgtttg tcggtgcctt tattgacaaa agcaagccct cagcacaggg taaataccgc
     3481 ccgtatttac tgaaatttgg gattcctctt gtggttttag cttcactact atttcttcct
     3541 cttgaatcag tacgtggctc agtgatttat gctttccttt cttattttgc cactgcgatg
     3601 gtttattctt ttgttaatat tccttatggc tcgctttctg cttctcttac tcgagacaca
     3661 gacgaaattg ctaaattaac cacaacccgt atgacaatgg caaatatcgc taatttgttt
     3721 gtttatacct tattcccact ttttgtgcag ttacttgcac cacatgccga attaaaaaac
     3781 accggctttt tcggtattga gctctattta ggtgattata tgtctgctgc tgcaggccct
     3841 gcttggttta aggtttatgc ggtctatatg gtaattggat ttgtggcgtt aatgctctct
     3901 tattttggta ctaaagagcg gattattcca acagcggaag aaacagataa agtcaagtat
     3961 tcagatttag ttaatgaact caaacgcaat cgtccattac agattttagg gttattcttc
     4021 ttaataggtt ttacttttat gttcttcgga aatacggtgt ggccttttta cctaaaatat
     4081 aatattgggc attctgagtg gattgcctcc gtcggtttat taggctctat ccctgggatt
     4141 ttccttgtat tcttatggcc gaaactacgt cgcactatcg gtaaaaagaa ctttttctat
     4201 ctatttttag ggattttcgt tctagggcaa ctttgtctat gggtatggca atttgactcc
     4261 ttcaaatact ctccaacctt aggctatatc ggtcgtttct tacaacaatg gggattaact
     4321 tctgctaccg gttttatgtg ggcgttagtg cctgaggttg ttacctttgg agagtttaag
     4381 tcgaaaaaac gtgtagcagg tattattaat gcgttaatgg gcttgttctt taaaatcggc
     4441 ttggcgttag gcggtattat tccgggttat attaatgcgg tttatggttt taacggctcg
     4501 cttttggctc aaaccgaaga ggctttattt ggtatcaatc tttcaatgat ttgggctcca
     4561 attattttag cgattattgc aggatttgtg atgagtcgct atccgttaag cgatactgat
     4621 attgatcata tcaacgaaaa aattactgct tattccaaat aatttgagta taggagaata
     4681 gattatgcaa attcaaaatc cagttttacc cggtttcaat ccagatccat ctattgtgcg
     4741 tgtgggtgat acttactaca ttgcaacttc tacttttgaa tggtttccgg gagtgagaat
     4801 tcacgaatcg aaagatttag tgcattggaa tttagttaaa aatgtcttag atacaaccac
     4861 gttgttagat atgcgaggca atccctcttc aggtggtatc tgggctcctg atctttctta
     4921 tgcggaaggt aaattttggt tggtttatac cgatgtaaaa attacagacg gtgcattcaa
     4981 agatatgact aactatctaa ctactgccaa agaaattcaa ggtccttggt cagctccgat
     5041 taaattgaat ggtattgggt ttgatgcttc gttgtttcac gatgatgatg ggcgaaaata
     5101 tttagtgcaa cagacttggg atcaccgtga atatcatcac ccctttgacg gtattacgtt
     5161 aacggaattt gatgttgcca caatgaaact caaacctgaa acccagcgta cgatttttga
     5221 aggtacagaa gtgaaattgg tggaaggtcc acatatttat aaaattaatg gcgaatatta
     5281 tctgtttgcg gcggaaggcg gcacagtatt tacgcatcaa gaagtagtgg ctcgttctaa
     5341 aacattggag gctcactctt tccaactggc tccaaataca ccgtttatta ctaattttga
     5401 tacacctaat tcttacctac aaaaacaagg gcacggttca ttagttgata ccccaagtgg
     5461 tgaatggtat tacgcttccc tcacagcaag accttggaat catgcaacag aatccgcaac
     5521 cgatcctcgt ggttggtcaa cattagggcg tgaagccgcc attcaaaaag tagaatttga
     5581 tcaagacggc tggccttata ttgtgggtgg acacggtgga aaaacctttg tagaagcccc
     5641 aaaagatgca atttttaccg aagctcctat tgatcattct caacacgatg agtttgatag
     5701 ctcaacgtta gatattaact ggaacactta agagtgcctt ttagtaaaga gatgggcgaa
     5761 gttggtaatg gtgttttaaa attgattggg cgaggctctc ttgcaaatac acacgattta
     5821 tcgctaatcg cacgccgttg gcaagccttc tattttgatg cggaaaccaa agtgaaattt
     5881 acaccgtttt cttatcaatc tatggcaggt ttaactaact attacaatca ctcacactgg
     5941 tcttggattt tcatcaccaa aaatgatcaa ggacaacagg tgattgaagt tgcagaaaat
     6001 aaaggtggac tcagaaatgg gcaatatact tcttacctta aagataaagc aattgtcatt
     6061 cctgatggaa cggagtatgt ttggtttaag actaaagttc ggaaagaaac ttaccgttat
     6121 gaatattcat tcgatggcaa acaatggcac actattccgg ttgaattaga tgcggcagtg
     6181 ctttcagatg attatgtgtt acgcagttat ggcggtttct tcacaggagc ttttgtcggg
     6241 ctggctgcag tggattactc cagttatgaa tctacagcag aatttagcta ctttgactat
     6301 aaagagttag gcgataagca acttacagac ggctcattct cttgggaaaa atctgaaagt
     6361 tgctttggct aaaatgagga gaatatcaca atgaaacaac gcagatttgg taaaaccaac
     6421 aaaatggtgt cagaaattgg gttaggcact tggcagcttg ggacgaaatg gggtgaaccg
     6481 ttcaatcatc aagaggcgat ggcaatttta gaaaccgctt atgagcaagg catcaatttt
     6541 attgataccg ctgatgtgta taacaacggg caaagcgaga aagccattgg tgatattttg
     6601 aaaaaatacc ctgacttttt ctatgtcgtt accaaatgtg gacgtgcgtt aaacccgcat
     6661 actgctgaaa tgtacacgcc acaggcaatc gaaaaatttg ttgatggtag tttaagccat
     6721 cttggcacgg aaaagttgga tatgattttg ctccactgcc cgccaacgtt ggtttatcgc
     6781 aatgatgaga tttttacgaa attggaacag cttaaagccg gcggaaaatt aatagactac
     6841 ggtgtgagta tcgaaacggc agaggaaggc ttgttggcaa tggagtatga cattgcggca
     6901 atggaagtta tcttcaatat gttccgctta aaaccattgg agcaactctt cccaaccgct
     6961 cagcaaaaaa atgtgggtat tatcgcccgt gtactgttgg caagtggttt gcttacaggc
     7021 agatataatg cctgcaccca atttggcaaa gacgaccacc gcacatttaa ccgcaatggt
     7081 gaagccttcg ataaaggcga aaccttttcg ggtgtggatt atcaactcgg tttacaagcg
     7141 gtggaagaat tgaaagccct attcggcacg gaagatttaa ttccttacgc cctacgttgg
     7201 gtattgatga atgaagctgt gagtacagta atcccgggag caagtaaggt ttcacaagta
     7261 attactaacg cagcggtgga taatttccct ccactcactg aaaatgaaat gcaaaaagtg
     7321 gaagatattt acaaccgctt aattcgccca agtgtgcatc atttgtggta atttatccaa
     7381 cgctataaaa aatggaactc taacttgagt tccatttttg tcaattttag ccttaaacct
     7441 ctaactgttt agaggttttc ttaaacagag tcaatgcaat aaacgcaaag gctaagaaac
     7501 aggaaaggat cagggcatag aaaccaccgt cccagccgaa catatcaacc agtttaccca
     7561 tcacataacc tgcacttgca gagcctaaca agtagccgaa taaaccggtt aaacctgttg
     7621 ctgtaccggt ggcaacacga ggcactaaat ccgctgcttg taaaccgatc atcatcaccg
     7681 gcccgtagat taagaaacca atcgccacta agcagatgtt gtcgattaac gggttgccgg
     7741 ctgggttttt ccagtacacc acaatcgcaa ttaacacacc agcaaggaat aacaacatcg
     7801 gcggcgcacg gtgacctttg aatactttgt cgcttaaata accgcttgca agcataccga
     7861 agataccggc atattcatac aagaagtacg cccagctctg tttatccaca gagaaatgtt
     7921 tcacttcttt taagtaagtc ggtgcccagt cgataatgcc gtagcgaatg aaatagacaa
     7981 acacgttggc aatcgcaatt gcccacagga atttgttgtt taaaatatat ttgtagaaaa
     8041 tgtctttgga agagagtgtg tgggcagact ccactttttc caccactttc tcgcctttgt
     8101 actcatcaat cggcggtaag ccttgagatt ccggcgtatc acgcatcaaa tagaacatca
     8161 caaacgccag tacaatcgca atcaacgccg gcagatagaa cagggattgc cacacgccaa
     8221 aaatagataa gcctaaaatc gccaacggcc cgattaaacc gccgcccaag ttgtgcgaca
     8281 cgttccacca gctccaccac acgccacgtt cagaggttga gaaccagttg gtcatcgttt
     8341 tcgcccccgg cggatagccc attccttgga accagccgtt taacgccgcc aaaatgatca
     8401 taattggaat agacgaaagc acgcccggta ctaaaccgaa caccagactc acaatcgctg
     8461 aaccgagcaa gccgatagtg ataaagtatt tcgggttaga acggtcggaa acgttaccca
     8521 taataaattt actgaagccg taagcgagtg ataatgccac gcccaccgta cctaagtctg
     8581 ctttggtgaa gccatattca tcaatcaaat acggcattgc caacgagaag tttttacgaa
     8641 ttaaataata tgccgcatag ccgataaaga cgcctgcaaa cacttgccaa cgtaatttgc
     8701 gatattccgc atcaactctg ctggcttcca ctttttgttc tgctggtgga gaggctttta
     8761 gaaaggaaaa cattttacta ctcctttaag aatgaaatcc tatggctact ataatctttc
     8821 atttggagca gaaaaagtgg atttttgaaa atttgtgagc aagattaaaa ttattttgag
     8881 cgaaaacgaa aagcggttga atttccttct ttttttgcaa aaaatttgag aaatctaacc
     8941 gcttgtttca cttaaaaatc agctatttct gctttttcaa aaattccacg cccgtatcag
     9001 ggaagtcagt aaagacgcct gttgcacccg ctttattcag cagggcatcg tacatttcat
     9061 cgacattttg gaaaaagtcc ggcagagcat ctttgcgcac ggtgtatggg tgaagttcca
     9121 ttttgtactt cgccaactct ttcactaatg gcgtgtaaac aatgttgccg actttagatt
     9181 tttctttatc caccaacata taccagcccg ggccgacacc atcggcatat tttgccactt
     9241 ctgccatcgc accgtctttg aacatccagt cgtagtcgta attaacccag ttgccgtttt
     9301 tatctttctc ttcggtttcg tgccaatcgg tgtaggcaac taactgcacc aattttacgt
     9361 ccatacccag tttcggtagt aattcggttt tgatccgttt cagctcgttg aaatcgaagg
     9421 tttgcaggta aattggtgag gttttgctgt cgtagccata ttttttcaac actttcaggg
     9481 tttcgagtgc aatgtcttta ccctctttat ggtgcaacca aggggcttta atttcaggat
     9541 agatcccaat ttttttgccg gttgatttct ctaatccttg aataaattcc agctcttctt
     9601 ccaaagtgtg aatggtgaaa tgggactgcc acatcgggaa gcggttcggg taaacttgtg
     9661 cctgcttgcc gtctttcacc tcaaaattct cggtcatttc aagggtttta atctcatcaa
     9721 gggtgaaatc caccacataa taacgaccgt ctgctcgttt acgctcaggg aattttttcg
     9781 ccacatcagt taaaccgtct aaaaagtggt cgtgaatcac aatcaggcgg ttatctttgg
     9841 tcatagcaag atcttgctct aaatagtccg cttgttgccc gaatgctaga gctttagatt
     9901 caagcgtatg ctcaggtaaa taaccgcttg caccacggtg ggcaatcact aatttatcag
     9961 attgttgtgc ataggcaccc aatgctgtcg cggacaatgc taatgcagca accaatgttt
    10021 tgaatttaaa tttcatagaa ttacctcata taatgtaaaa ataaaaggaa gaacaaaaat
    10081 gtccttcctt ttgataagtt atttattatt taccatagtg atcttctaat tttgctttgt
    10141 gcttgccttc ttcaaccatc acaatgaagg ttaagagaat ggctaataca ccaccaccga
    10201 tcatcacgta gaaaccgccg tcccagccaa atttatctgc tgcccagcca actactgcag
    10261 aggctgccac tgtaccacct aagtaaccga ataaaccggt gaaacctgct gctgtacctg
    10321 ctgctttttt cggtgcaagt tcaagggcgt gtaagccaat caacattacc gggccgtaga
    10381 ttaagaaacc gatagtggtc atccaaatga agtccattaa ttgatatggg ttttcatacc
    10441 aagcatggcc tgcatattgt gcaatttcag attccggtgt cgcagggttg agccagaacg
    10501 caaccactgc tgcggtggta agaatcataa agatgaaacc ggttaaacca cgtttgcctt
    10561 tgaacagttt gtcagagacc caaccacaga gtaatgtacc tggaatcgca gctaattcat
    10621 aaatggtata tgcccatgct gtacctttaa tgttgaagtg ttttacttcg cctaagtaca
    10681 ccggcgacca tttcaatacg ccatagcgga ttaagtaaac gaagacattc gcaatggcaa
    10741 tgtaccataa cagtttgttt ttcagcacat aagtcacgaa aatctctttg gtgcttaaat
    10801 cgtgttcata ggttttttcg ttatagtcgt cagggtagtc gttacgccat ttttcaactg
    10861 gcggtaaacc gcaagattgt ggagtatctt tcatcacaag gtagattgga atagctgcga
    10921 tcattgctgc aattcccggg aaataaaggg cttgttgcca tacatctttt gccgttgctt
    10981 ctacaccata ctcactgaaa tagagcgcac ttgctaatag caccatcgcc cccggcacca
    11041 taccgcctaa gttgtgagcg gtattccaga tagaaacgat agtaccacgc tcagatttag
    11101 accaccagtg aaccattgtt cgtccacacg gtggccaacc cataccttga aaccagccgt
    11161 ttaagaagat cattacccac attactaaaa tgcttgatgt tgcccaaggc attaagccca
    11221 taagggtcat acatagacct gaaagtaaca atccggtcgg taaaaatact ttcgggttag
    11281 agcggtcgga cattcctgcc atcacgaatt ttgataaacc gtaggccagg ccggcagctg
    11341 taccaatcac accgagatcg gtttttgtat acataccagc ttcaatcaag cctttttgtg
    11401 ctaagtcgaa gtttgcacgc acaaaatagt aagcggcata gccgaagaaa attcctgcaa
    11461 acacttgcca acgcaagaat ttatatcttg catcgacttt gtccgccggt aattccgcaa
    11521 tgtgcggagc gggtttaaat ggaccaaaca tagtattctc caatattact tagtaaaacc
    11581 tctgagtaat tcctttttag gaaattaggg aagcaataat aaatgaaaat aaaaaatgac
    11641 aaagttattt tttataaaaa tgtgattcat ttcacaaaaa atttacctta aaccacaaaa
    11701 aatgattgaa aaagaaaatc aaacatcagt atctctagcg ataaaactct cttcttatgt
    11761 acagagaaag aaccata
//
LOCUS       DANLRK010000077        11714 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.077, whole genome shotgun sequence.
ACCESSION   DANLRK010000077 DANLRK010000000
VERSION     DANLRK010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 11714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11714
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.077"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            11..268
                     /locus_tag="PXA33_001465"
     CDS             11..268
                     /locus_tag="PXA33_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL5120789.1"
                     /translation="MKNLRLSIHSSEHIWLRELFLKRRLELGLTQRTLGEKMGVLYSF
                     IGKVETGDRRLDIFEFIAYCKGLDLDPLSVLQEIEQQFPHK"
     gene            complement(315..935)
                     /locus_tag="PXA33_001466"
     CDS             complement(315..935)
                     /locus_tag="PXA33_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HDL5120790.1"
                     /translation="MKLWYSTTSPFARKALAVIKHHQLDDKVEMLRVTKAMDPNSPHN
                     QDNPLGRIPALQRNCGRWLFGSLLISEYLDQKGNNTPLLPKEGKPRWAVLALHNLADG
                     IMENTMPTLLQERMARPENEWWVSRHEQLMERNIRSFRQLEEALQEFGTELNLGTLTA
                     VALIDWWAFRLDKIGYDLAKNYPNLTAWAEEMNNKYAILAETKPSM"
     gene            complement(937..1590)
                     /locus_tag="PXA33_001467"
     CDS             complement(937..1590)
                     /locus_tag="PXA33_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01621 family pseudouridine synthase"
                     /protein_id="HDL5120791.1"
                     /translation="MTPTLPICYQHRDFIIINKPEGLSVHKDEAEIGLTELVARQLNV
                     KQVWLVHRLDKITSGLLILALNKEAAARFYHLFEQHKIYKTYWALSDKKPKKKQGRIV
                     GDMQKSRNGAWKLCHSKENPAVTQFISHSVEDSLRHFILQPKTGKTHQLRVAMKSLGS
                     PILGDKLYSGSQADRVYLHAYQLDFEYENEKISVQALPTSGHFWQKICKSNHIKHGE"
     gene            1639..2298
                     /locus_tag="PXA33_001468"
     CDS             1639..2298
                     /locus_tag="PXA33_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDL5120792.1"
                     /translation="MAVTIYLVRHGRTVWNLEGRLQGSGDSALVEEGIIGAKKTGIAL
                     KHIPFTAAYSSMQKRAQDTANYILAENERSNIPHFHHKGLNEFDFGSWEGMKSVDLQE
                     NDEYWVMKRTPAEYLAKANGGERFEQLYQRVTQAFNQIAELHQNDGKVLIVSHGMTLT
                     LLTAVLKGIAWQDFRNEEKHSFVFNTAITKVEVENGKVTVLEFNNTDHLDNVPTSQYK
                     R"
     gene            complement(2335..3381)
                     /locus_tag="PXA33_001469"
     CDS             complement(2335..3381)
                     /locus_tag="PXA33_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HDL5120793.1"
                     /translation="MSKQIFNEEELKTTEHFEPKQEFDLENAIIEEEEKAVEAELIIE
                     ESLKPSRFWIRVLLATLALFGVAVIAQSIQWLIDTFQQREWIYFAFSIVFFIVSLLGV
                     GAIISEWRKLVYLRNHQKNQQMSNQLLLEQLPSNDGEQAVKFCENIANQLNHLPSIAQ
                     AEQLWKSELNEAYNAKEVLYLFSENVLKPIDKQVKQMISRNAAENAVIVAVSPLAIVD
                     VLLMAARNIALVNKITKAYGMELGYISRLKLFKMVLKNMVFAGATEIATDVGMDFFSQ
                     NLTAKLSLRAAQGIGVGLLTARLGIKAMEFCRPVAFQANEKPRISAIRQELLTSVKNT
                     VFAKTETKEKVFSD"
     gene            complement(3381..4334)
                     /gene="dusC"
                     /locus_tag="PXA33_001470"
     CDS             complement(3381..4334)
                     /gene="dusC"
                     /locus_tag="PXA33_001470"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HDL5120794.1"
                     /translation="MNPIPNVILAPMQGVLDPFVRNLLTSVNDYDLCISEFVRVVDQR
                     LPKKTFYRLAPELYQGGLTDSGTPVRVQLLGQHPQWLAENAALAIELGSHGVDLNCGC
                     PSKTVNGSNGGASLLKDPELIYRATKAMREAVPSDKIVSVKVRLGWDSVSQCFEIADA
                     VEQGGANEITVHGRTKVDGYRAERINWQAIGEIQQRLSIPVIANGEIWDFESAKNCQK
                     ITACKALMMGRGALNTPNLSKVVKYNVPKMAWNEVLQLLFKYVQMENQFDTGFYHVAR
                     IKQWLRYLDKEYPQAVELFDILKTEHGYDGLKAHIEKAVEE"
     gene            complement(4412..5038)
                     /locus_tag="PXA33_001471"
     CDS             complement(4412..5038)
                     /locus_tag="PXA33_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HDL5120795.1"
                     /translation="MDSRSHIIPLFLAIVITVIAVWSGLNPTDRAVWYAEVIPIFIVF
                     ALLIITYPKFQFSGLAYFLMSLWLIMHLIGAKYTFANVPFEWANQFLTPILGEDRNHF
                     DRVAHYIIGFYSFPMAEWLLRKRKCGMGVAFFFSLFFIMSVAAAYEIIEWQYAVIEGG
                     NAGVEFLGSQGDIWDAQKDMLADTLGALTALMIYLIARPDLRIHDRHF"
     gene            complement(5038..5895)
                     /gene="djlA"
                     /locus_tag="PXA33_001472"
     CDS             complement(5038..5895)
                     /gene="djlA"
                     /locus_tag="PXA33_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005609757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HDL5120796.1"
                     /translation="MQFIGKIVGFFLGYQLFHGLFGGILGAFIGHLADKKLYELGSVR
                     SSLFGKNLTRQSLFTQTTFAVLGHIAKAKGRVTEADIELARALMARLKLDSAAQQLAQ
                     NAFTLGKEPDFPLRQVIQEFREACGARTDLLRFFVEVQMQAALQDGQLDVNEQQILFT
                     IAETMGMSRFQFEQMIAMIMAAQQFRNGGYYQQNGSYHQQSQGGYQGSYQQPAQPSIE
                     AAYTVLGVTAQDDQNTVKRAYRKLMNEHHPDKLAAKGLPDEMMELAKEKAQQIQAAYD
                     LICKVKEWK"
     gene            complement(5943..6653)
                     /locus_tag="PXA33_001473"
     CDS             complement(5943..6653)
                     /locus_tag="PXA33_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDL5120797.1"
                     /translation="MWKNRQLFFGYAENRLLLLSRSITGLLGILCGVYIIDHLVLSDV
                     DMIGKLTSFILIILSAIFLKERASLLQWILCLIAFIGALFIIKPAVDIRFMPYLVGVI
                     GSVFAAIAYLCLRLLAKTKKVESPNTIVFFFSAFSTLILLPFVAIDYVVLTNLQTIYL
                     VLAGVTSAVGQFCVTTAYKYAAAKDISIYSYASVLFSAILGLLFFEQYPDMLSWLGYA
                     IIFVSSWLMFVLTKKQSK"
     gene            complement(6889..7374)
                     /locus_tag="PXA33_001474"
     CDS             complement(6889..7374)
                     /locus_tag="PXA33_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HDL5120798.1"
                     /translation="MKKFAKVLMVAVPAFALAACSSSSDDAAANAAANAAANAAANAA
                     GEFGGLTAEQLKAQYNTVYFGFDSYAVEGEYRQLLDAHAQLLAKTQGNVTIAGHADER
                     GTPEYNIALGQRRADAVKGYLAAKGANNTSTVSYGEEKPAVLGHSEADYAKNRRAVLE
                     Y"
     gene            complement(7401..8687)
                     /gene="tolB"
                     /locus_tag="PXA33_001475"
     CDS             complement(7401..8687)
                     /gene="tolB"
                     /locus_tag="PXA33_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HDL5120799.1"
                     /translation="MKLFSRISGVIGIFSALLTTQVVADSDVRISIDEGVSMAQPIAV
                     VPFKASGVPADVAQIISDDLRNSGKFTPLERSQMPAQPGSAAEVVADQWSAIGIDNIV
                     VGQVSSTGGGYNVAYQLIDTLGTSGTAGGVIAQGSFIVPAAQIRQGAHTVSDEVFEKL
                     TQIRGAFRTKIAYVVQRGVSSYELRVSDYDGFNAFTITTSKEPLMSPEWSPDGSRLAY
                     VTFENKKAQVVVHDIRSGARRVVAALKGHNGAPSFSPDGSRIAFASNQDGELNIYVVG
                     SGGGTPSKLTANAGNNTEPSWSPDGSTIYFTSDRAGSPQVYRMSSSGGGASPMGGSGS
                     YNAKVSSDGKNLIMIAGDRVVKRDLASGGTEVLSSTFLDESPSISPNGIMVIYSSTKG
                     TSKVLQLVSADGRFKANLPGAGGHYKFPAWSPYLTK"
     gene            complement(8703..9926)
                     /gene="tolA"
                     /locus_tag="PXA33_001476"
     CDS             complement(8703..9926)
                     /gene="tolA"
                     /locus_tag="PXA33_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HDL5120800.1"
                     /translation="MKSQQDRLELAVIVSILLHILLIGLLLLGSLFTKTELAAAGGSG
                     GDSDSFEAVMVDTGQVAAEYGQLLAEKKGSKASKPKVEPKEEAVEEDVEDKPTPEEIQ
                     KQKQAELAAIQEKQRQQEIERQQKLQEQQKQEQAKQEELKKQQAEEATRKKAAEAAKL
                     KADAEAKRLEAAAKQAEEEKKAKEALEKKLEQQKKAEAEKKLKEQKELKEQQAKEAKE
                     AKEKAEKEAKAKAEKEAKEKAEKEAKAKAEKEAKEKAEKEAKAKAAAEAKAAQAKNNK
                     ALDDFLNSGDIGGGSSKGGNKNSAGSQGSGGTSGLGQGTNVDGNAYGQRIKKLIQSRY
                     RVDPSFAGKQCDVKIFLSRDGTITNYQVISGDKAVCDAAVSAIVATRKVPPAPSDAVY
                     NMFKSPTLDFSLKVK"
     gene            complement(9940..10377)
                     /gene="tolR"
                     /locus_tag="PXA33_001477"
     CDS             complement(9940..10377)
                     /gene="tolR"
                     /locus_tag="PXA33_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HDL5120801.1"
                     /translation="MSRRLKRNDIKSEINIVPFLDVLLVLLLIFMATAPIISQSVEVN
                     LPEDTHSQSVSNEDKTPVILEVAGVGEYKLKIDGNYVQSNGQENINESEVVAYAGEQF
                     QKDNNTLFLVAGDKVVPYEEIMKGITLLKDAGVKSVGLMTEGK"
     gene            complement(10519..11199)
                     /gene="tolQ"
                     /locus_tag="PXA33_001478"
     CDS             complement(10519..11199)
                     /gene="tolQ"
                     /locus_tag="PXA33_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HDL5120802.1"
                     /translation="MQTEFSLLSLFLEASIVVKIVMLILIVFSILSWAVIIQRSKILT
                     KAKKDSLAFEDRFWSGEDLNRLHQGLENRRDALNGSEQIFYVGFKEFTRLQQANPDAP
                     ESIIQGSTRAMNLALNREMESLENYIPFLGTVGSISPYIGLFGTVWGIMHSFMGLSAV
                     KQATLQSVAPGIAEALIATAIGLFAAIPAVMAYNRLNLRLGKVEQGYVNFIDEFTTLL
                     HRQAFSKR"
     gene            complement(11221..11622)
                     /gene="ybgC"
                     /locus_tag="PXA33_001479"
     CDS             complement(11221..11622)
                     /gene="ybgC"
                     /locus_tag="PXA33_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HDL5120803.1"
                     /translation="MDFPIRVYYEDTDAGGVVYHSNYLNFFERARTEFLRQQHFSQQE
                     LFSQSLAFVVKRIEIDYKLPARLDDLLNVETTLSELKKATLIFKQRLWKDGDCLSEAN
                     VTVACVDLIKMKPVAIPDDIRQALQKSYFSI"
BASE COUNT         3382 a         2635 c         2144 g         3553 t
ORIGIN      
        1 tataggtgca atgaaaaatc ttagattatc aatacattcc agcgagcata tttggttaag
       61 agaactattc ttaaaacgcc gtttggaact agggctaaca caacgcacat taggagaaaa
      121 aatgggcgtt ctttactcat ttatcggaaa agtagaaaca ggagatcgca gattagatat
      181 ttttgaattt atcgcttatt gcaaagggtt agatttagat cctttatctg tattgcaaga
      241 aattgaacaa caatttcctc ataaataaag gcattgagtt ataactcaat gcctttattt
      301 tctatgtcaa atctctacat gctcggctta gtctctgcca gaatggcgta tttattattc
      361 atttcctctg cccaagcggt taaatttggg taattttttg caagatcgta gcctatctta
      421 tctaagcgga atgcccacca gtcaattaat gctactgcgg ttaatgtgcc taaattcagc
      481 tctgtgccaa actcttgcaa tgcctcttct aattgtctga acgaacggat attgcgttcc
      541 attaattgct cgtggcggct gacccaccat tcattctcgg ggcgagccat acgttcttgt
      601 aataaagttg gcatcgtatt ttccataatg ccgtccgcca aattatgtaa tgccaacact
      661 gcccagcgag gtttaccctc tttaggtaac agtggagtgt tattgccttt ttggtcaaga
      721 tattcgctaa ttaacaaact accgaaaagc caacgtccgc agttgcgttg tagggcagga
      781 atacgtccta acgggttatc ttgattatgt ggcgaatttg gatccattgc ttttgtcacc
      841 cgcaacattt cgactttatc gtctaattgg tggtgtttaa tcaccgcaag ggctttgcgg
      901 gcgaaagggc tggtggttga gtaccaaagt ttcatactat tctccgtgtt tgatgtgatt
      961 ggatttgcaa attttttgcc aaaaatgacc gcttgtcggc aaggcttgca ctgagatttt
     1021 ctcattttcg tattcaaaat caagctgata agcgtgcaaa taaacacggt cggcttggct
     1081 acctgaatag agtttatcac ctaaaatcgg gctgcccaaa cttttcattg ccactcttaa
     1141 ttggtgagtt ttgcctgttt tgggttgcaa aataaaatgc ctgagcgagt cttcaacaga
     1201 atgagaaata aattgcgtta ctgccgggtt ttccttactg tggcataatt tccacgctcc
     1261 atttctgctt ttttgcatat cgccaacaat tctaccctgc tttttcttcg gctttttatc
     1321 gcttaatgcc caataggttt tgtagatttt atgctgttca aataaatggt aaaaccttgc
     1381 tgccgcctct ttattcaacg ctaaaatcag taaacctgag gtgattttat ccagacggtg
     1441 aaccagccaa acctgcttta cattcagttg gcgagccact aattccgtta agccgatttc
     1501 agcctcatct ttatgcacac tcaagccttc cggcttattg ataataataa aatcacgatg
     1561 ttgataacaa ataggcaatg ttggggtcat tttgctataa tccttccaat ttttgactat
     1621 tttagcaagg aaattacaat ggctgttacc atttatttag tacgacacgg cagaaccgtg
     1681 tggaatttgg aaggcagatt acaaggctcg ggcgattccg ctttagtaga agaagggatt
     1741 atcggagcga agaaaaccgg cattgcgtta aaacatattc cgtttaccgc cgcctattcc
     1801 agtatgcaga aacgggctca agataccgca aactacattt tagcagaaaa tgaacgcagt
     1861 aatattccgc attttcatca taaaggcttg aatgaatttg attttggcag ctgggaagga
     1921 atgaaatctg tcgatttgca agaaaatgat gaatattggg tgatgaaacg tacaccggca
     1981 gaatatctgg caaaagcaaa cggtggcgag cgatttgagc aactttatca acgggtgact
     2041 caggcgttca atcaaatcgc cgagctacac caaaatgacg gcaaagtgct gattgtttca
     2101 catggtatga ccttaacctt actcacggct gtgttaaaag gcattgcgtg gcaagatttc
     2161 cgcaatgaag aaaaacatag ctttgtgttc aatactgcca ttacgaaagt agaagtagag
     2221 aatggcaaag taacggtgct ggaatttaat aataccgacc atttggataa tgtgccaaca
     2281 agccaataca aacgataata ttaagggcgg aacattgtcc gcccatagca ttttctaatc
     2341 agagaacact ttttctttcg tttctgtttt agcaaatacc gtatttttga ccgaagtaag
     2401 caactcctga cgaatagctg aaattcttgg cttctcattc gcttgaaaag caacaggacg
     2461 acaaaattcc attgctttaa tgccaaggcg agcagtgagt aaacccacac caataccttg
     2521 tgcggcacgg agcgataatt tcgccgtaag gttttgcgag aagaaatcca ttccaacatc
     2581 agtagcgatt tcggttgcac cggcgaacac catatttttc agcaccattt tgaacagttt
     2641 taagcggctg atataaccca attccattcc ataggctttg gtaattttat tcactaaagc
     2701 gatatttctt gccgccatta acaacacatc aactattgcc agcgggctaa ccgccacaat
     2761 caccgcattt tctgccgcat tgcgggaaat catctgctta acctgtttat ctatcggctt
     2821 tagcacattt tcgctaaaca gatatagtac ctctttggca ttataagcct cgttcagttc
     2881 gcttttccaa agttgctcgg cttgagcaat gctcggcaag tgattaagct gatttgcaat
     2941 attttcgcaa aatttgaccg cttgttcgcc atcattggaa ggcagctgtt ccagcaataa
     3001 ttggttactc atttgttgat ttttctgatg atttcgcaaa tacaccaact ttcgccattc
     3061 ggaaataatc gccccaacgc ccaacaagct gacaataaaa aagactatcg agaacgcaaa
     3121 atagatccac tctctttgct ggaacgtatc aatcaaccac tgaatacttt gagcaattac
     3181 cgccacgcca aacaaggcaa gtgtggcaag tagtactcga atccaaaagc gggaaggctt
     3241 aaggctttct tcaataatca gctccgcctc aactgctttt tcctcttctt caataatcgc
     3301 attttcgaga tcaaactctt gtttaggttc aaaatgctcg gttgttttta gctcttcttc
     3361 attaaaaatt tgcttactca ttattcctcc actgcctttt caatatgtgc ttttagacca
     3421 tcatagccgt gttcggtttt caagatatca aataattcta ccgcttgagg atattcttta
     3481 tccaaataac gtagccattg tttgatgcga gcaacgtggt aaaaaccggt atcaaactga
     3541 ttttccatct gaacgtactt aaacaatagc tgtagcactt cattccacgc cattttcgga
     3601 acattatatt tcaccacttt gcttaaattc ggtgtgttta acgccccacg ccccatcatt
     3661 aatgccttgc aagcggttat tttttggcaa tttttcgccg attcaaaatc ccaaatttcg
     3721 ccgttggcga taacaggaat agacagccgt tgttgaattt cgccaatcgc ctgccaatta
     3781 attctttctg ctcgataacc atcaactttg gttcgcccgt gaacggtaat ttcattcgct
     3841 ccgccctgtt ctaccgcatc ggcaatttca aaacattggc taacggaatc ccaacctaat
     3901 cgcactttta cggaaacgat tttatcgctt ggcacagctt cacgcatcgc tttggttgca
     3961 cgataaatca gttccggatc ttttaataac gaagccccac cattgctgcc atttaccgtt
     4021 ttagaggggc agccacagtt gagatctacc ccgtgtgaac caagctcaat ggcaagagct
     4081 gcattttctg ccagccactg tgggtgttgc cctaaaagct gtacccgcac tggtgtgccg
     4141 gaatcggtta atccgccttg atatagctca ggggctaggc gataaaaggt tttcttcggt
     4201 aaacgttgat cgaccactcg cacaaattcg gaaatacaga gatcgtagtc attcactgaa
     4261 gttaaaaggt tgcggacaaa agggtctaaa accccttgca tcggggcaag aataacgtta
     4321 ggaattggat tcattggtaa aataaaaaat acaagcggtt attttttctc aacattttgc
     4381 aaaaaattgg caagaaacaa ccgcttgaag gttaaaaatg tctatcgtga atgcgcaaat
     4441 cggggcgagc gatcaggtaa atcatcagtg cggttaatgc cccaagagta tccgcaagca
     4501 tatctttttg agcgtcccaa atatcccctt gcgagcctag aaattccacg ccggcattgc
     4561 caccttcaat cacggcatat tgccattcaa taatttcgta agctgccgcc accgacataa
     4621 tgaaaaacag cgagaagaaa aatgctacgc ccattccgca cttgcgttta cgcagtaacc
     4681 attctgccat cgggaagctg taaaaaccga taatgtaatg tgctactcga tcaaaatggt
     4741 tacgatcttc acctaaaatc ggggttagaa attgatttgc ccactcaaac ggcacattgg
     4801 caaaggtata ttttgccccg atcaagtgca taatcagcca aagcgacatc aaaaaataag
     4861 ctaacccact aaactgaaat ttagggtaag tgataattaa aagggcaaaa acgataaaaa
     4921 tcggtatcac ttccgcatac caaactgcgc ggtctgtcgg atttaaccct gaccaaaccg
     4981 caatcacggt aataacaatc gctaaaaata gagggatgat atgcgagcgt gaatccatta
     5041 tttccactct ttcactttac aaattaaatc gtacgccgct tgaatttgtt gagctttctc
     5101 ttttgccaac tccatcatct catcaggcaa acctttagcg gctaatttat ccgggtgatg
     5161 ttcgttcatt aacttacgat aagcccgttt cactgtattt tgatcgtctt gtgcggttac
     5221 gcctaatacg gtataggctg cctcaatgct tggttgtgca ggctgctgat aactgccctg
     5281 atagccacct tgagattgct gatgataact cccattttgc tgataatagc caccattgcg
     5341 gaattgttga gctgccataa tcattgcgat catctgctca aattggaagc gagacattcc
     5401 cattgtttcg gcaatcgtaa acagaatttg ttgctcgttc acatcaagtt gcccatcttg
     5461 caaggctgcc tgcatttgca cttcgacaaa gaaacgtaat aagtcagttc ttgccccaca
     5521 ggcttctctg aactcttgaa tgacttggcg taacgggaag tcaggctctt tacctaaggt
     5581 aaaggcattt tgcgctaatt gctgggctgc actatctaat tttaagcgag ccattaacgc
     5641 tcgggctaat tcaatatccg cttcagtaac acgccctttt gctttcgcaa tatgccccaa
     5701 caccgcaaaa gtggtttggg tgaaaagcga ttggcgagtt aaatttttac cgaaaagact
     5761 tgaacgaact gaacctaatt catagagttt tttatcggct aaatgcccga taaaagcgcc
     5821 aagtatccca ccaaataaac cgtgaaaaag ctgatagccc aagaaaaaac cgactatttt
     5881 tccgataaat tgcatttttt tatcctttta tagaaatgaa taaacgttat tctaacaaaa
     5941 ttttactttg attgcttttt agttaataca aacataagcc agcttgagac aaaaataatt
     6001 gcataaccta gccaactcaa catatccggg tattgttcaa agaacaacaa ccctaaaatt
     6061 gcactaaata gcacagaggc ataactataa attgagatgt ctttcgccgc cgcatattta
     6121 taggcggtag ttacacaaaa ctgcccgacc gctgaagtta cccctgctaa cactaagtag
     6181 attgtctgta aattggttag cactacataa tcaatggcga caaaaggcaa taatatcaag
     6241 gtcgaaaagg cagaaaaaaa gaaaacaatg gtattaggtg attctacttt tttggttttt
     6301 gccaataacc ttaagcataa ataggcaatg gctgcaaaca cagagccaat cacgccaacc
     6361 agataaggca taaagcgaat atcaactgcc ggtttgataa taaaaagtgc cccgataaag
     6421 gcaataaggc ataaaatcca ttgcaggaga gatgctctct cttttaaaaa aatcgcagaa
     6481 agaataatta aaatgaacga cgtgagtttg ccaatcatat cgacatcact tagcacaagg
     6541 tgatcgatga tatatacacc acataaaatc cccaataacc cggtgatgga acggcttaat
     6601 aataacaaac gattttctgc ataaccaaag aataattggc gatttttcca cacaatataa
     6661 ccgcttatca atgctgtgcg ttgcgaaaaa tcgctttttg tgcaatcggt acatcccccg
     6721 aaagacgaag aaaagcagcc ataatcgcaa agcttagtgc cgacaaaagc acacaaaaaa
     6781 tggctttaaa taacggcgag agcgaattcc aaaaattcat agcataaagg cttttaataa
     6841 aaataaagcc ctgattattc agggcttcaa tttagaatca actaagaatt agtattctaa
     6901 tactgcacga cggtttttcg cgtagtcagc ttctgagtga cctaatactg ctggtttctc
     6961 ttcaccgtaa gaaacagttg aagtgttgtt agcaccttta gctgctaagt aacctttaac
     7021 tgcatctgcg cgacgttgac ctaatgcgat gttgtactct ggagtaccac gctcatcagc
     7081 gtgaccagca atagttacgt taccttgagt ttttgctaat aattgagcgt gagcgtctaa
     7141 taattgacgg tattcacctt caactgcata gctgtcaaaa ccgaagtata cagtgttgta
     7201 ttgtgctttt aattgttcag ctgttaaacc accgaattcg cctgctgcat ttgctgctgc
     7261 atttgctgct gcgtttgctg ctgcgtttgc tgctgcatca tctgatgagc tgctacatgc
     7321 tgctaatgcg aaagctggta ctgctaccat taatacttta gcaaattttt tcatttgttg
     7381 ctcctaatag agaaatatta ttatttagtt aagtatggtg accatgcagg gaatttataa
     7441 tgtccacctg cgccaggcaa attagcttta aaacgcccat ctgcagacac caattgtagc
     7501 actttactcg tacctttggt agagctataa ataaccataa tgccattcgg tgaaatactt
     7561 ggactttcgt ctaagaaagt tgaactcagt acttctgtgc cacctgaagc gagatcgcgt
     7621 ttcacaactc tatcaccggc aatcatgatt aaattcttac catcagatga tactttcgca
     7681 ttatagctac cgctaccgcc cattgggcta gcaccaccac ctgatgaact cattctataa
     7741 acttgaggag aacctgcacg gtcagacgta aagtagattg tgctaccatc tggagaccag
     7801 cttggttcag tattatttcc agcattagcg gtcaatttac ttggtgtacc accgccacta
     7861 cctacaacat aaatatttaa ctcaccatct tgatttgagg caaacgcaat gcgagaaccg
     7921 tctggagaga atgatggcgc accattatgt cctttcaatg ctgccactac acggcgagca
     7981 cctgaacgaa tatcgtgaac aacaacctgc gcttttttat tttcaaatgt cacataagct
     8041 aatctgctac catctggtga ccactctggc gacattaaag gctctttact ggttgtaata
     8101 gtaaaggcat taaaaccatc ataatcagat actcgtaatt catacgatga tacgccacgt
     8161 tgaaccacat acgcaatttt agttctaaat gcaccgcgaa tttgggttaa tttttcaaat
     8221 acctcatcac ttactgtatg cgcaccttga cgaatttggg ctgccggaac aataaatgaa
     8281 ccttgggcaa tgacaccacc cgctgtaccc gatgtaccaa gagtatcaat taattgataa
     8341 gcgacattat aaccgccacc tgtgctactc acttggccaa ctacaatatt atcaataccg
     8401 atagcactcc attgatctgc caccacttcc gctgctgatc ccggttgagc aggcatttga
     8461 gagcgttcaa gcggtgtaaa tttaccacta tttcgcaaat catcagaaat aatttgtgca
     8521 acatctgccg gcacaccgct ggctttaaat ggcacaaccg caatcggctg agccatactt
     8581 acaccttcat caatagaaat tcgcacatca gaatcagcaa ccacctgagt tgtcaataat
     8641 gctgaaaaga tgcctattac acccgatata cgagagaata atttcatttg tagtcctcta
     8701 aattatttaa cttttaaact aaaatcaagt gttggtgatt taaacatatt atataccgca
     8761 tctgatggtg caggtggtac ttttctagtt gccactattg cacttaccgc tgcatcacat
     8821 actgctttat cgccactgat aacttgatag ttagtaattg tgccatctcg acttaagaat
     8881 atcttaacat cacattgttt tccggcaaaa cttggatcaa cccgatatcg agattgaatt
     8941 aatttcttaa ttcgttgacc atatgcatta ccatctacat tagtaccttg ccctaagcca
     9001 cttgtaccac cagaaccttg tgatcctgct gagtttttgt taccaccttt acttgaacca
     9061 ccaccgatgt ctccactatt gaggaaatca tcaagtgctt tattgttttt cgcttgggcg
     9121 gcttttgcct cagcagctgc cttcgcttta gcttctttct cggctttctc tttggcttct
     9181 ttttctgctt ttgctttggc ttctttctca gccttttctt ttgcttcttt ttctgccttg
     9241 gctttagctt ctttttctgc tttttccttc gcttctttag cttcttttgc ctgctgttcc
     9301 ttcagttctt tttgctcttt taactttttc tcagcttccg ctttcttctg ctgttcaagc
     9361 ttcttttcaa gtgcttcttt agcttttttc tcttcttcag cttgtttagc agcagcctct
     9421 aaacgcttag cttctgcatc tgctttcagt tttgctgctt cagctgcttt tttacgagtt
     9481 gcttcttctg cttgctgctt tttaagctct tcttgcttag cttgttcttg cttttgctgt
     9541 tcttgtagtt tttgctgacg ctcaatttct tgctgacgct gtttctcttg aatcgccgct
     9601 aactcagctt gtttctgttt ttggatctct tccggagtcg gtttatcttc cacatcttct
     9661 tcaacagctt cctctttagg ctctaccttt ggcttcgatg cttttgagcc ttttttctct
     9721 gccaataact gcccatattc agcagccact tgcccagtat ctaccatcac agcttcaaag
     9781 ctatcgctat cgccaccact gccaccagcc gcagcgagtt ccgtcttagt gaaaagagac
     9841 ccgagcaata acaacccgat taacaatata tgcaataaga tcgaaacaat aaccgctagc
     9901 tctagtctat cttgttgaga tttcacacgt tatccttggc tatttacctt ctgtcattaa
     9961 acctaccgat ttcactccgg cgtctttaag tagtgtaata cctttcataa tttcttcata
    10021 aggtactact ttatcaccgg caactaaaaa taaggtattg ttatcttttt gaaattgttc
    10081 ccctgcatag gcaacaactt ctgattcatt aatattctct tgcccattac tttgcacata
    10141 gttaccgtca attttcaatt tatattcacc aacacctgcc acctccaaaa tcactggagt
    10201 tttatcttca ttagaaactg attggctatg cgtatcttcc ggtaaattaa cttctacact
    10261 ttgactgata attggggccg ttgccataaa aatcagcaat aataccaaca atacatcaag
    10321 aaatggaaca atgttgattt ctgatttaat gtcattacgt tttaaacgac gagacatata
    10381 ttcctcttta caggtgctta gcaccttaat tgatctgaaa taaccggcac agtgtcccgg
    10441 tctcgaataa tgcggttaca agcggtcttt tttacgaaaa actttacaaa ttttcattaa
    10501 aaatcaaccg cttgtagatt atctttttga aaaagcctga cggtgtaata aggtcgtgaa
    10561 ctcatcaatg aaattcacat acccttgctc aactttacct aaacgtaaat tcaaacggtt
    10621 atatgccatt actgcaggaa tcgccgcaaa taaaccgata gccgttgcaa ttaatgcttc
    10681 tgcaataccc ggtgcgactg attgtaaagt tgcttgctta actgcactta atcccataaa
    10741 ggagtgcata attccccaaa ccgttccgaa taaaccaata tatgggctga ttgaacccac
    10801 tgtgcctaaa aacggaatgt agttttctaa actttccatt tcacggttta atgctaagtt
    10861 catcgcacgg gtagaacctt gaataatcga ttctggcgca tctgggttcg cttgttgtaa
    10921 acgggtaaat tctttaaacc caacataaaa gatttgctct gaaccattta atgcatcacg
    10981 acgattctct aaaccttggt gtaaacggtt tagatcttcc cctgaccaga atcgatcttc
    11041 aaaagcaagg gaatcttttt tggctttagt taaaattttg ctgcgctgaa taatcaccgc
    11101 ccaagacaaa attgagaata caataaggat cagcatcacg atctttacca caatacttgc
    11161 ttctaaaaat aacgaaagaa gactaaattc agtttgcatt aataaaactc cgaatagttt
    11221 ttaaatagaa aaataacttt tttgtaatgc ttgacgaata tcatcaggaa ttgcaaccgg
    11281 cttcattttg attaaatcaa cacaagcgac cgtaacattt gcctcactca gacaatcgcc
    11341 atctttccac aatcgttgtt taaagataag ggttgctttc ttcaattctg agagtgttgt
    11401 ttccacattt aataaatcat ctaaacgagc agggagctta taatctattt caatcctctt
    11461 aacaacaaaa gcaagggatt ggctaaaaag ttcctgctgt gagaaatgct gctgtctcaa
    11521 aaattccgtt cttgcccgct caaaaaaatt cagatagttg gaatgataaa ctactccgcc
    11581 ggcatcagta tcttcataat aaacacgaat cgggaaatcc ataagcatta tttctctcat
    11641 tcaaaatgaa aaaacgccca aaatttaggc gcaaattgtg agatatttta ggatcagaaa
    11701 tattgttatg caag
//
LOCUS       DANLRK010000078        11659 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.078, whole genome shotgun sequence.
ACCESSION   DANLRK010000078 DANLRK010000000
VERSION     DANLRK010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 11659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11659
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.078"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(33..752)
                     /locus_tag="PXA33_001480"
     CDS             complement(33..752)
                     /locus_tag="PXA33_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253715.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HDL5120804.1"
                     /translation="MKLKNFLTILPLAVATAVTSAETHTVEHKDKPASFNFSTEVSRT
                     VEKDVMQASVYSQKSGNSLPELKKFVSENLNRVLAEAKKHPSIEVQAEGVTNHVNYNN
                     KGNIDGWDTSGSIQLKSKDFDAMAAVLENLGKDVAIRYIDFSVSPEKMASLEDEMTLE
                     IISKFQHKADVIQKALGAKTYKLVNINIQTPNDGGRPYYEHRAMYAVSSMAQSKSMDE
                     IPLEAGKTTISATASGSVEFE"
     gene            complement(810..1547)
                     /gene="tsaA"
                     /locus_tag="PXA33_001481"
     CDS             complement(810..1547)
                     /gene="tsaA"
                     /locus_tag="PXA33_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HDL5120805.1"
                     /translation="MNSTSNQLTPIQLNPIGFVESPYDEKFSVPRQPNLVAEGKGILR
                     LIPPYNTPDAVRGLEKFSHIWLIFQFHHIPEREWHATVRPPRLGGNERVGVFASRATH
                     RPNPLGLSKVKLEAVVAGGSEVYLKLGSVDLVNGTPIFDIKPYIAFADSEPQAVSGFA
                     QQKPQARLAVRFSEEAKQAVEFCRNFAKFGIEQPLSFLADVIAQDPRPAYQQGKPSER
                     VYGMNLAGYNIRWQIENDTQAIVLSIE"
     gene            1727..2635
                     /locus_tag="PXA33_001482"
     CDS             1727..2635
                     /locus_tag="PXA33_001482"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HDL5120806.1"
                     /translation="MNKNSLFSIWFTTARPKTLPLALASIIVGSALAHWAGKFDVVTT
                     LLAFITTILLQILSNFANDYGDHVKGSDTKERIGPLRAIQHGVIGGEQLKKAVIILSL
                     LSFVSGVALSAYAFETVQDLFFFIGLGIVSIIAAITYTVGKKAYGYLGLGDLFVLIFF
                     GFVAVIGVFYLQAHSLPIMILLPAFGCGLLSVAVLNINNLRDINQDRQAGKNTLIVRI
                     GSQNGRKYHVALLVLAVISYLIFAISEFEHWYSFIFLLSVPLLAKHARFVYQHKDPKE
                     LYPILGQMAGLALLTNLLFSLGIVLG"
     gene            complement(2711..3697)
                     /locus_tag="PXA33_001483"
     CDS             complement(2711..3697)
                     /locus_tag="PXA33_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HDL5120807.1"
                     /translation="MKKSLLALLIGSVFLISACEDGKMTQTVLEAEKQIVQLGTELKN
                     AQADLAKKEAEVAELSGVKAENEKLKSELENAQQNTALNVEIVKIFDKKEVIKHQVDP
                     KQEFAIEESTVSAFVSLPKTNFDWLNQLLINQAYDYNEEQGRKLKNPTEEEFKKHREA
                     VYQGLVESVKTEPAIGYDDHISSAFIGQRNNIATFTMMHYTYMGGAHGMHYTQYINVD
                     LAKKAIIGLNDLISPKNQVQLKDILWENYSSVRVDENGNYNGFADKKEFRISDQFYFT
                     PHGIAFVYPPYELGAYAEGDIEVEADWYIINKLLNSEYQRGEKDGFFEELDN"
     gene            complement(3791..5662)
                     /gene="rpoD"
                     /locus_tag="PXA33_001484"
     CDS             complement(3791..5662)
                     /gene="rpoD"
                     /locus_tag="PXA33_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HDL5120808.1"
                     /translation="MENQADQQLEQQSQLELLISQGREQGYLTLSEVHDHLPEELVDA
                     EQIEDIIQMINDMGIQVLEAAPDADDLLLAENIADEDVVEEATKVLSTVESELGRTTD
                     PVRMYMREMGTVELLTREGEIDIAKRIEEGINEVQCAIAEYPEALSGLLACYAQVETG
                     EMRLSDLVTAFVDPNILEEAPLEDIEAALGEDEESSDVPDVDDENEDENGDSSSDSSD
                     DSSIDPELAREKFEALRSQHEKVLLVIQKHGRTSKKSKEQIEILSHIFREFRLVPKQF
                     DLLVASMQKLMKQVRTEERQIQRYAVEYAGMKKADFLKAFQGHELSEAWIEKAINAKK
                     NAAPKLANYVDNIRVNIANLQNLEQTSGLTIAQIREIGGRISNGELKARRAKKEMVEA
                     NLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAIT
                     RSIADQARTIRIPVHMIETINKLNRISRQCLQEMGREATPEELAERMGMPEDKIRKVL
                     KIAKEPISMETPIGDDDDSHLGDFIQDESLELPLDSATAESLRMATNEVLEGLTPREA
                     KVLRMRFGIDMNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSETLRSFLDD"
     gene            complement(5921..7669)
                     /gene="dnaG"
                     /locus_tag="PXA33_001485"
     CDS             complement(5921..7669)
                     /gene="dnaG"
                     /locus_tag="PXA33_001485"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDL5120809.1"
                     /translation="MKGTIPRSFIDDLIARTDIVEVINSRVKLKKAGRDYQACCPFHH
                     EKTPSFSVSQSKQFYHCFGCGVHGNVITFLMDYDKLEFPEAIEELAAMHGLEVPRENV
                     IQRDGKPQANFKTKRNLYELMEAVAKFYQQNLSHQIEPQSYLQQRGLSEEIIERFEIG
                     FAPNAFDAALRTFGQSQEDIQKLLDTGVLTKNDSGRIYDKFRNRVMLPIRDKRGRVIA
                     FGGRVLPNDEQGAKYMNSPETVIYHKGSELYGLYQALKVNETPDYLFVVEGYMDVVAL
                     AQFGVDNAVASLGTATTGEQIQQMFRCTEQIVCCYDGDRAGRDAAWRALENALPYLQD
                     GRQLKFIFLPDGEDPDTFVRSQGKEGFEQYVAQNAKPLSDFLFDSLLADVDLSSKEGK
                     SKLAALAVPLINRIPGEMLRVYLRNILGQKLGILDPTQIEALLPTKKAAEKPLHQAVK
                     MKRTPMRLLIALLIQNPYFVKFVPDVSALASLKDPGFTLLTDIVATCQQNAGISMGGL
                     LEHYREDPQYKIVEALANWDHLELPENIERTFIDTLDFLYAKLVELRKEELIAKDRTV
                     GLTDDEKKEYALLMFQ"
     gene            complement(7823..8038)
                     /gene="rpsU"
                     /locus_tag="PXA33_001486"
     CDS             complement(7823..8038)
                     /gene="rpsU"
                     /locus_tag="PXA33_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005701713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HDL5120810.1"
                     /translation="MPVIKVRENESFDVALRRFKRSCEKAGILAEVRAREFYEKPTTI
                     RKREKASLAKRHAKRNFRENARNTRLY"
     gene            complement(8186..8782)
                     /locus_tag="PXA33_001487"
     CDS             complement(8186..8782)
                     /locus_tag="PXA33_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HDL5120811.1"
                     /translation="MSLLPMNEITSLNQLDLQKSYSYADYLLWKFKERVEIIKGKIVA
                     MSPAPTRFHQRISMKMTAKFLEVFDNHQCQIYSAPFDVRFPDENGKVKTVVQPDLCVI
                     CDVSKLDERGCVGAPDLVVEILSPGNTKREMKDKYELYQEQGVKEYWIVSPETRTIQI
                     FVLENGKYIGIQPVAEDEPATSVVFPALSFSTEKLFEL"
     gene            8924..10180
                     /locus_tag="PXA33_001488"
     CDS             8924..10180
                     /locus_tag="PXA33_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HDL5120812.1"
                     /translation="MHLNFKKIDNSHIALFYNVLVATFFVTVLTFKKGYSYVPMALGI
                     IATLSFLFYRFKLKQKWLLDKEDKFFIFSLIGYFLTFAMSAIFNGDGIREIDNPIQIL
                     LLIPLIILFRLYPIKKETVLHFIPIGALVAGCIAIYQRFILNLKFPFPEIMRIQSGNI
                     AILLSLFSIAIAFYWLSKKRIKTGIAYLVFAMFGLFASILTGARGGWLAFIPICFFIL
                     FLNLRQLNKKAIFLILLALISSLSFFISKPEFGIQKRYNAAKNDIIVFLEKGNRHSSL
                     GARFDMWENALIGIREKPLLGHGSTGYETFKKKQIETKQMAKTTLRFNSLHNQYLEAF
                     VKRGVVGFMALMAILIIPFVIFAKRLKAKNREVKCMAILGIVHIISHSFFFLSQSFLA
                     HNSGSLFYFFVLILLYHLVKQNEEII"
     gene            complement(10282..11289)
                     /gene="rfbB"
                     /locus_tag="PXA33_001489"
     CDS             complement(10282..11289)
                     /gene="rfbB"
                     /locus_tag="PXA33_001489"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HDL5120813.1"
                     /translation="MIILITGGAGFIGSALVRYLIEHTPHTVINVDKLTYAGNLASLE
                     SVAHHERYHFEKADICASGQISEIFGKYQPDVVMHLAAESHVDRSISGAADFIQTNIV
                     GTYTLLEAARAYWDTLPEPRKSAFRFLHISTDEVYGDLAKSEELFTEKTPYNPSSPYS
                     ASKAASDHLARAWHRTYGLPTVITNCSNNYGAYQYPEKLIPLMILNALEGKSLPVYGN
                     GLQVRDWLFVEDHIRALYLVLQKGQIGETYNIGGHNEKTNIEVVRSICSILQEMVPNN
                     PLVVGQYADLITYVQDRPGHDFRYAIDTSKIEKELDWKPKETFESGLRKTVEWYLANR
                     N"
     gene            complement(11286..>11659)
                     /locus_tag="PXA33_001490"
     CDS             complement(11286..>11659)
                     /locus_tag="PXA33_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HDL5120814.1"
                     /translation="YFYRVKTSESILDKIERFKQRSEGYPVNSILNDIFGARIIVSSE
                     VIQQVMESLDYWQEKYGLKNWYLRDKDEYVGIHIYFKNSSNFYYPWELQIWDKKDAEK
                     NIQSHIKYKRNFVNQISYSPL"
BASE COUNT         3269 a         2533 c         2147 g         3710 t
ORIGIN      
        1 aaaaatacaa ctctatacaa atctatacat ttctattcaa attccacact tcctgaagcg
       61 gtagctgaaa ttgtggtttt acctgcttct aacgggattt catccataga tttgctttgt
      121 gccattgatg aaactgcata cattgctctg tgttcataat atggtctacc accatcattt
      181 ggagtttgaa tattgatatt aactagctta tatgttttcg cccctaaggc tttttgaatc
      241 acatcggctt tatgctgaaa tttgctgata atttccagcg tcatttcatc ttcaagggac
      301 gccattttct ctggcgatac actaaaatca atgtaacgaa tcgccacatc tttacctaaa
      361 ttttccaaca ccgctgccat tgcatcaaaa tctttgcttt tcaactgaat gctaccagag
      421 gtatcccaac catcaatatt ccctttgttg ttataattaa cgtgattagt cacaccttca
      481 gcttgtactt caatcgatgg atgttttttt gcctcagcca atacacgatt caggttttca
      541 gatacaaatt ttttcagctc cggcaaggag ttacctgatt tttggctata cacacttgcc
      601 tgcatcacgt ccttttccac cgtgcgagag acttcggttg agaaattaaa agaagccggt
      661 ttatctttat gttccacggt atgagtttcc gctgaagtta ccgctgttgc gactgcaagc
      721 ggtagaatgg taagaaaatt tttcaatttc ataatcagtc cttaaaaggg ttaaaaatca
      781 tttcttagat ctcacttgcg taggaaagtt cattcaatcg aaagcacaat cgcctgtgta
      841 tcattttcaa tttgccagcg aatattgtag ccggcaaggt tcataccata aactcgctca
      901 ctaggtttcc cttgttgata cgccgggcga gggtcttggg cgatgacatc ggcaagaaaa
      961 ctaagcggct gttcgatgcc aaattttgca aaatttcggc aaaattcgac agcttgtttt
     1021 gcctcctcgc taaaacgcac cgctaatctt gcttgtggtt tttgctgagc aaaaccggaa
     1081 accgcttgcg gctcgctatc tgcaaatgca atgtaaggct tgatgtcaaa aattggtgtg
     1141 ccgttaacta aatccacgct gcctaatttc aaataaactt cactcccccc cgccactacc
     1201 gcttctaact tcactttcga taagccaagc ggattcggac gatgggtggc acggctggca
     1261 aagaccccga cccgttcgtt cccgcccaag cgaggcggtc tgacggtggc gtgccactct
     1321 ctttcgggaa tgtgatggaa ttggaaaatc aaccaaatat gactaaattt ctccaagcct
     1381 ctgactgcat cgggggtatt gtaaggcgga attaaacgta gaatgccttt cccctccgct
     1441 accaaattcg gctggcgagg cacggaaaat ttttcatcat aggggctttc cacaaaaccg
     1501 atagggttta attgaatcgg ggttagttga tttgaggtag agttcattac gcttttttct
     1561 gttaaaataa tataaaatat tggacataat gatatgaaat tgaggatggt gcaagcgtcc
     1621 acgcttgtac caattcgttt taccatttaa cacaagagtg gacgcttgca ctatcgctta
     1681 aatccttaac atcattgtaa atgaaattac gctaagatta ttctctatga acaaaaattc
     1741 tctcttttcg atttggttta ccaccgctcg cccgaaaact ttaccgctgg cgttagcttc
     1801 aattattgtg ggttcagcac ttgctcattg ggcagggaaa tttgatgttg ttaccacctt
     1861 actcgcattt atcaccacca ttttgttgca gatcctgtct aacttcgcca atgattacgg
     1921 cgaccacgtc aaaggctctg acaccaaaga acgaatcgga ccgctacggg cgattcaaca
     1981 cggtgttatt ggcggtgaac aacttaagaa agccgtgatt atcttaagcc tactctcgtt
     2041 tgtttccggt gttgccttaa gtgcctatgc ttttgaaaca gtgcaggatc tgtttttctt
     2101 tatcgggctt ggcatcgtct caattattgc cgccattact tacaccgtcg gcaaaaaagc
     2161 ctacggctat ttggggctgg gcgatctatt tgtgctgatt ttctttggct ttgtagcggt
     2221 aatcggcgtg ttctacttgc aggctcatag cctacctata atgattctgc tccctgcttt
     2281 cggttgcgga ttgctgtctg tagcagtgtt aaatatcaat aacttgcgtg atattaacca
     2341 agaccgacaa gctgggaaaa acactctcat tgtacgaatc ggtagccaaa acgggcgaaa
     2401 ataccacgtt gcattgttag tgctagcggt catttcttat ctgatttttg caatttctga
     2461 atttgaacat tggtatagct tcattttttt gctgtctgta ccgttactgg caaaacacgc
     2521 tcgttttgtc tatcagcata aagacccaaa agaactctac ccaattctag ggcaaatggc
     2581 agggctggcg ttgctcacta atctattatt tagtttgggc attgtgttag ggtaagttgc
     2641 aaaattttaa caaaactaaa ccgcttgtaa taaaggatta caagcggttg ttttttatct
     2701 aaatttacaa ttagttatct agttcttcaa agaatccatc tttttcgcca cgttgatact
     2761 ccgaattgag caacttatta ataatgtacc aatccgcttc aacctctata tcgccttctg
     2821 cataagcacc taattcgtat ggcggataaa caaaagcaat gccgtgaggg gtaaagtaga
     2881 attggtcaga aatacggaac tcttttttat cagcaaatcc attatagttg ccattttcat
     2941 ccacacgcac gctactgtaa ttttcccaaa gaatgtcttt cagttgaact tgattttttg
     3001 ggctgattaa atcatttaag ccaatgattg cttttttagc taaatctaca ttgatatatt
     3061 gcgtatagtg cataccgtga gccccaccca tataggtgta atgcatcata gtgaatgtag
     3121 caatattatt gcgttgcccg ataaaagcac tggaaatatg atcatcatag ccaatagctg
     3181 gctcggtttt cacactttct accaaccctt gataaaccgc ttcacgatgc tttttaaact
     3241 cttcttcggt tggatttttc agctttctgc cttgttcttc attgtaatca taggcctgat
     3301 taatcaaaag ctggttaagc caatcaaaat tggtttttgg tagagacaca aacgcactta
     3361 ctgtactttc ttctattgca aattcttgtt tcggatcaac ttggtgctta atcacctctt
     3421 tcttatcaaa gattttcaca atttcaacat ttaacgctgt gttttgctga gcgttctcta
     3481 actctgattt taatttttca ttttctgctt tcacccctga aagttctgca acttcagcct
     3541 ctttttttgc taaatcagcc tgagcatttt ttaattctgt gcctaactga acaatttgtt
     3601 tttcggcttc taataccgtt tgggtcattt tgccgtcttc acaagctgaa atcagaaata
     3661 ctgagccaat taaaagtgct aatagtgatt ttttcatctc tccctctttt gtaaaaaata
     3721 cggtaaatat caccgcttgt tatacagatt aaaacaaaat cccctatacc atcaggcata
     3781 ggggaaatga ttagtcgtct aagaaactgc gtaaggtttc cgaacggctc gggtggcgaa
     3841 gtttgcgcag tgctttcgcc tcaatttggc ggatacgttc acgagttacg tcaaattgtt
     3901 taccaacttc ttcgagtgtg tggtctgtgt tcatatcaat accaaaacgc atacgcaaca
     3961 ctttcgcttc acgcggggtt aagccctcta acacttcgtt cgttgccata cgcaggctct
     4021 ccgcagtggc ggaatccaat ggtaattcaa ggctttcgtc ttgaatgaaa tcacctaaat
     4081 gtgaatcgtc atcgtcaccg attggtgttt ccattgaaat cggctctttg gcaattttca
     4141 atactttgcg gattttatct tccggcattc ccatacgttc tgccagttct tccggcgttg
     4201 cctcacgccc catttcttgt aaacattggc gtgaaatacg gttgagtttg ttgatagttt
     4261 caatcatatg caccggaata cgaattgtac gggcttggtc agcaatcgaa cgggtaatcg
     4321 cttgacgaat ccaccaagtt gcataagtcg agaatttata accacggcgg tattcaaatt
     4381 tatccaccgc tttcattaaa ccgatgtttc cttcttggat taaatctaag aactgcaagc
     4441 cacggttagt gtattttttc gcaatcgaaa tcaccaaacg taagttagct tccaccatct
     4501 cttttttcgc acggcgggct tttaactcac cattggaaat acgcccgcca atttcacgaa
     4561 tttgagcaat ggttaagcct gatgtttgct ctaaattctg taagttagca atattgacac
     4621 ggatattatc cacataatta gctaatttcg gtgctgcatt tttcttggca ttaatcgctt
     4681 tttcaatcca cgcttcgctt aattcgtgcc cttggaatgc tttcaagaaa tccgcttttt
     4741 tcatgccggc atattccact gcatagcgtt gaatttgacg ttcttcagtg cgaacctgct
     4801 tcatcagttt ttgcatagaa gcaaccaata agtcaaattg cttcggcacc aaacggaatt
     4861 cacggaaaat atgagataaa atttcaattt gctctttcga ttttttactg gttcgcccgt
     4921 gtttttgaat gaccaataac actttttcat gttggctgcg tagggcttca aatttttcac
     4981 gggcaagttc cgggtcaatt gagctgtcat cactgctgtc tgaactgcta tcaccgtttt
     5041 catcttcgtt ttcgtcatct acatcaggca catcagaaga ttcttcatct tcacctaaag
     5101 cagcctcaat atcctctaac ggtgcttctt ctaaaatatt aggatcgaca aatgctgtca
     5161 ctaaatcaga aaggcgcatt tcaccggttt cgacttgagc atagcaggct aacaggccac
     5221 ttaaggcttc cggatattcc gcaatcgcac attgaacttc gttaatccct tcttcaatgc
     5281 gtttagcgat atcaatctca ccttcacggg ttaaaagctc aactgtcccc atctcacgca
     5341 tatacatacg cacagggtca gtagttctac ctaactctga ttctacggtt gaaagcactt
     5401 tggtggcttc ttctaccaca tcttcatcgg cgatattttc agcgagtagt aaatcatccg
     5461 catcaggggc agcctctaaa acttggatac ccatatcgtt gatcatctga ataatatctt
     5521 cgatctgctc cgcatccacc aactcttccg gtaagtggtc gtgcacttca gaaagcgtta
     5581 aatatccctg ttcacgccct tgactgatca gaagttctaa ttgagattgc tgttctaatt
     5641 gttgatctgc ttggttttcc atagtctatc cgtttattat gcgagcaaaa atagtagtgg
     5701 attataccac taaatttggt tcgacttcat aaattcacaa aaaaattcta tttttctaat
     5761 ttacacatta ctttgatgct tatgagaaca gagaggtgta gcaagagaga taaataactt
     5821 tctttttaga tagaattaat tattttcttc gttcatcact tctgccaaat ctcccctaac
     5881 ccctctttgc taaagagggg aattattcga gcttaaaatt ctactgaaac atcaaaagag
     5941 catactcttt tttctcatca tcggttaagc ccacggttcg gtctttggca attagctctt
     6001 ctttgcgtaa ttccactaat ttggcgtaga gaaaatcgag agtgtcaata aatgttcgtt
     6061 cgatattttc gggtaactct aaatgatccc aatttgcgag ggcttcaaca atcttatatt
     6121 ggggatcttc ccgataatgt tcaagtagcc cgcccatact gatacctgca ttttgctgac
     6181 aagttgcgac aatatcggtt aataatgtaa agccaggatc tttcaaggag gctaaagcac
     6241 ttacatcggg tacgaatttc acaaaatacg gattttgaat caataacgca atcaacagcc
     6301 gcattggcgt acgtttcatt ttgaccgctt gatgtaaagg cttctctgcc gcttttttcg
     6361 tcggtaacag agcttctatc tgcgttgggt ctaaaatgcc taatttctgt cctaaaatat
     6421 tgcgtaaata cactcgcagc atctcgcccg gaatacgatt aattaacggc acagctaaag
     6481 cagcgagctt ggattttccc tcttttgaag agagatccac atccgccagc aaactgtcaa
     6541 ataagaaatc gcttaatggc ttggcattct gtgccacgta ctgctcaaat ccttctttac
     6601 cttgagaacg aacaaaggtg tccgggtctt cgccatcggg caagaaaata aatttcagtt
     6661 ggcgaccgtc ttgcaaataa ggcaacgcat tttctaacgc ccgccaagct gcatctcgac
     6721 cggctctatc gccatcataa caacagacaa tttgctcggt gcaacgaaac atttgctgaa
     6781 tttgttcacc ggtagttgcc gtgcctaaag aggcgactgc attgtccacg ccgaattggg
     6841 caagggcgac cacgtccata tagccttcca ccacgaacag ataatccggc gtttcattga
     6901 ctttcagtgc ttgatacaag ccatataact cactgccttt atgataaatc accgtttcgg
     6961 gcgagttcat atatttcgcc ccttgctcat cattcggcaa cacccgccca ccaaaggcaa
     7021 ttacccgtcc acgcttatct cgaatcggta acattacccg attgcgaaat ttatcgtaaa
     7081 ttcgaccgct atcatttttg gttaaaacgc ccgtatcgag caatttctga atatcttctt
     7141 gtgattgccc aaaagtgcgt agggctgcat caaaggcatt cggagcaaaa ccaatttcaa
     7201 agcgttcgat aatctcttca gacaaacctc gttgctgtaa atagctttgt ggctcaatct
     7261 ggtgcgataa attctgctgg tagaacttcg ccaccgcttc catcaattca taaagattac
     7321 gcttagtttt aaaattagct tgaggcttgc cgtcacgttg aatcacattc tctctcggta
     7381 cttccagccc gtgcatagct gccagttctt caatcgcttc cggaaattcg agtttgtcat
     7441 aatccatcaa aaaggtgatg acattgccgt gtacgccaca gccgaaacaa tggtaaaact
     7501 gttttgattg gcttaccgaa aaggaagggg ttttctcgtg atgaaaagga cagcaggctt
     7561 gataatctcg cccagccttt ttgagtttga cccgactgtt gatgacctca acaatatcgg
     7621 tgcgagcgat aagatcatca ataaatgaac gtggaattgt gcctttcatt gatttcctcc
     7681 tgctctttga atgatacaag cggagcaatt ttgcaaattt tttgcaaaaa acaaccgctt
     7741 gaaatagaaa cgttaaaaac accaaaaccg tgagcctaac ggaatcacgg tttcgatata
     7801 gtttgctaac cagcctaact aattagtata aacgtgtgtt acgtgcgttc tcacggaaat
     7861 tacgtttagc gtgacgttta gcaagtgatg ctttttcacg tttacgaatt gtagttggtt
     7921 tttcgtagaa ttcacgagca cgaacttcag ctaagatacc cgctttttcg caagagcgtt
     7981 tgaaacgacg taatgctacg tcaaatgatt cattttcacg aactttaatt actggcatat
     8041 gccatcacct cagaaaaata tgattaaatt aaaccgcaca caattttggc ggcatagaac
     8101 aaaaaaggta gtgcattcta ccttaagcac tcacataagt aaagtagtat tgtggcataa
     8161 aaccgccgta ttatgttgca tttttctaca gttcgaataa tttctcggtc gaaaaactta
     8221 atgccggaaa gaccaccgaa gttgccggtt catcttctgc caccggctga ataccgatat
     8281 atttaccgtt ttccaacaca aaaatctgaa tagtgcgggt ttcgggagaa acaatccaat
     8341 actctttcac cccttgttcc tggtaaagct cgtatttatc tttcatctcc cttttggtat
     8401 tacccggaga aaggatttcc accactaaat ccggtgcccc tacacagcct ctttcatcta
     8461 acttactgac atcacaaatt acacaaagat caggctgcac tacagttttg acttttccgt
     8521 tttcatcggg aaaccgaacg tcaaaaggcg cagaataaat ctggcattgg tgattgtcaa
     8581 aaacctctaa aaattttgct gtcatcttca tagaaattcg ctgatgaaaa cgagtaggag
     8641 ccggagacat cgccacaatt ttccctttga taatctcaac ccgctcttta aatttccata
     8701 gcaaataatc agcgtaactg taactttttt gtaagtccag ttgatttaag ctggtaattt
     8761 cgttcattgg taataagctc ataccgcctc ctttatcaga aatataaaga tattatgccg
     8821 aataatccct caaattcaac aaaactttcg ctaaaaagag atagataacc tttataccct
     8881 tagtataaaa tgacagcaat tttcattgtt ttaggatatg tcgatgcacc ttaacttcaa
     8941 aaaaatagac aactcacata ttgctctttt ctataatgta ctagtcgcca cattttttgt
     9001 cactgtttta acctttaaga aaggttatag ttatgtgcca atggcattag gtattatcgc
     9061 tacacttagt ttcttatttt atcgtttcaa attaaaacaa aagtggttac ttgataaaga
     9121 ggataaattt tttattttct cgcttattgg ctattttcta acgtttgcaa tgtctgctat
     9181 ttttaatggc gatggcattc gtgaaattga taatcctatc cagattttat tgcttatccc
     9241 tttaatcatc ctctttagac tttacccgat taaaaaagaa actgtattgc actttatacc
     9301 gattggagcg ttggtagctg gttgtatagc aatatatcag aggtttatat taaatcttaa
     9361 atttcctttc cctgaaataa tgcgtattca atcggggaac atagcaatat tattatcact
     9421 atttagtata gcaattgcct tttattggct ttcaaaaaaa agaattaaaa ctggcattgc
     9481 ctacctggtt tttgccatgt ttgggttatt tgccagtatt ctaacaggag caagaggcgg
     9541 ctggcttgca ttcattccca tttgcttttt tatcctattt ttaaatttaa gacagctaaa
     9601 taagaaagct attttcctga ttctattagc acttatatct tcactctcat tttttatctc
     9661 caaaccagaa tttggcatac aaaaacgata taatgcagct aagaatgata ttatcgtatt
     9721 tttagaaaaa ggaaatagac attcatctct gggagctcgt tttgatatgt gggaaaatgc
     9781 gcttatcgga atccgtgaaa aaccgctatt aggacacggt agcacaggct atgaaacatt
     9841 caaaaagaaa cagattgaaa caaaacaaat ggcaaaaaca acgcttaggt ttaatagctt
     9901 acacaaccaa tacctagaag cctttgtgaa aagaggtgta gttggtttta tggcgctaat
     9961 ggctatttta attattccat ttgttatttt tgccaaacga ttaaaggcaa aaaatagaga
    10021 ggtaaaatgt atggcgatat taggcattgt acatattatt tcgcattcat tctttttcct
    10081 aagccaatca tttttagccc ataattcagg ctcattattc tatttctttg tgttaatttt
    10141 actctaccat ttagtcaaac agaacgaaga gataatatga taaaacaata gaagaaagtt
    10201 gggaatataa ccgaggtggt aacttcatct tctaccacag gataaatgtc gggatatttc
    10261 ctctttttct gcttcttact actaattccg attagccaaa taccattcca ctgttttacg
    10321 caagccactt tcaaaagttt ctttcggttt ccagtcgagt tccttttcga tttttgaagt
    10381 atcaattgca tagcggaaat cgtggccagg gcgatcttgc acataagtaa ttaaatcagc
    10441 atattgtcct acaacaagcg ggttatttgg caccatttct tgcaaaatag aacagataga
    10501 acgaaccact tcgatattgg ttttttcatt atgcccgcca atattgtagg tttctccgat
    10561 ttgccctttt tgcaacacca aatagagggc acgaatatga tcttccacaa acagccaatc
    10621 tctgacctgc aagccgttgc cgtaaacagg tagggattta ccttctaagg catttaaaat
    10681 cattaacgga ataagttttt cgggatattg gtatgcccca taattattgg aacagttggt
    10741 gatgacagtc ggtaaaccgt aggtgcgatg ccaagcccga gccagatgat cgctggcagc
    10801 tttagatgcc gaataggggc tacttggatt ataaggggtt ttctcggtaa ataattcctc
    10861 gcttttggct aaatcaccat agacctcgtc ggtcgagata tgtagaaagc gaaacgccga
    10921 tttccttggc tcaggtaacg tgtcccaata ggctctcgct gcctctaata aagtataagt
    10981 gccgacaata ttggtttgaa taaaatctgc tgccccagaa atagagcgat caacatggct
    11041 ttccgctgct aagtgcatta ccacatcagg ctgatatttg ccaaaaatct cagaaatttg
    11101 accgcttgca caaatatctg ctttttcaaa atggtagcgt tcgtgatgtg cgacactctc
    11161 caatgaggct aaatttcccg cataggtgag cttatccaca ttgataactg tgtgtggagt
    11221 atgttcaatc agatagcgaa ctaatgcaga tccgataaaa ccggctccgc cagtgatgag
    11281 gatgatcata aaggtgaata tgaaatttga ttcacgaaat ttcgtttgta tttaatatgg
    11341 ctttgaatat tcttttcagc gtctttttta tcccaaattt gcaattccca aggataataa
    11401 aagttacttg aattcttaaa ataaatatga atacccacat attcatcttt atctctcaaa
    11461 taccaatttt ttagtccata tttttcttgc caataatcta atgactccat tacttgctga
    11521 atcacttcac tagaaacaat aatacgagct ccaaaaatat cgttgagaat gctatttact
    11581 ggataacctt cactacgctg tttaaaacgc tcgattttat ctaaaatcga ttctgaggtt
    11641 tttactcggt aaaaataag
//
LOCUS       DANLRK010000079        11382 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.079, whole genome shotgun sequence.
ACCESSION   DANLRK010000079 DANLRK010000000
VERSION     DANLRK010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 11382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11382
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.079"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            <1..1974
                     /locus_tag="PXA33_001491"
     CDS             <1..1974
                     /locus_tag="PXA33_001491"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HDL5120815.1"
                     /translation="FQNNPLLAQLKQQIEASKEYVAGVVKASDKAFGFLECDKKSYFI
                     PPAEMKKVMHGDSVKAVVKRDGDKEQVEIDSLLEPMLDRFIAQVRLNKEGKLQLAVDH
                     PSIKQTIPANTHKKATEKLENGDWVVAQLKTHPLRDDRFFFAQVTQFICKESDNFAPW
                     WVTLARHEQPREPVANEKNYELHDELAREDLSHLYFTTIDSPSTQDMDDALFIEPIKQ
                     NGKRTGWRLVVAIADPTAYIPENSHIEKAARQRCFTNYLPGFNIPMLPRELSDDLCSL
                     VPNEKRPALVGFIETDLNGNLIKETTFTSAWVESKDRLAYDDVSDYLEGVENAWQPQS
                     EETKQQIEWLHQFTQARIQWRAENALLFKEQGDYTFELNEDGSVKDIHVEYRRIANQM
                     IEESMILANICCAKFLSQHAKTGVFNTHSGFDPKNYELVKSFLLQTLASDENRDELAE
                     RYSAERLATLEGYCQMQRDIQDYPEKFLELRLRRYLTFAEFKAEVAPHFGLGITDYAT
                     WTSPIRKYGDMVNHRLIKQVLLGKQSQAVEESVLSRLQEARRQNRLVERDIADWLYAR
                     YLAPMVEQAVEFECEIADVSRGGLRAKVIKNGAQIFVPFSSLHDNKEEMEYRQEELAL
                     YIKGEKAYQIGQAVKVKLTEVRLETRSIVGNII"
     gene            2066..2866
                     /gene="apaH"
                     /locus_tag="PXA33_001492"
     CDS             2066..2866
                     /gene="apaH"
                     /locus_tag="PXA33_001492"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HDL5120816.1"
                     /translation="MATYIVGDLHGCFNEFQLLLEKVNYNPKQDELFLTGDLVARGED
                     SLACLRFVKDSANNAKTVLGNHDLHLLGIKRVKPNDKIDAIFEAKDRLDLQNWLRNQP
                     LVIHHPKHQFLLTHAGISPEWDLATTLACAKEAEKVLQSDNYADYIAQMYENKPERWS
                     EDLQGVERWRYIINVFTRMRFCYTDKRLDFDCKLPLEQAPQELLAWFDLDNPLFTQKN
                     IIFGHWASLMGKCTRPNIYALDTGCAWGSHLTMLRWEDKQIFTQKRLK"
     gene            2930..3006
                     /locus_tag="PXA33_001493"
     tRNA            2930..3006
                     /locus_tag="PXA33_001493"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2964..2966,aa:Val,seq:gac)
     gene            3234..4232
                     /locus_tag="PXA33_001494"
     CDS             3234..4232
                     /locus_tag="PXA33_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL5120817.1"
                     /translation="MATIIKNGNKYRAQVRKKGIYKTATFSTKTEANRWAYALEAQID
                     AGEYNTTPNILFAELIDKYLKEVTPTKRGKREERYRLLRIAGSNLGNIPLPALTKEHL
                     RQWQNQRLQEVQPVSVSRERATLSALFTKAMEWDYLKENPLKTLEKIKTPPARTRRYS
                     DEEIDKLIFVSGYSPNQPPLLMQQRAGAALLFAIETAMRAGEIAGLKWEHINLQARTA
                     FLPQTKNGYSRTVPLSTKAIAILNQLAQLEPNKQNSVFQLTSRSLDSNFRKLKQKANL
                     HNADLHFHDTRREALTRLSKYLTVMELAKVSGHRDLSILQNTYYNPDISELVSKLQ"
     gene            4510..4692
                     /locus_tag="PXA33_001495"
     CDS             4510..4692
                     /locus_tag="PXA33_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HDL5120818.1"
                     /translation="MAMSRNEIQAKSEAKRGIKQKSFKLPLEVIAEIEVLSQKLNIPQ
                     NQLIIQAIQQFKQNNP"
     gene            complement(4962..5252)
                     /locus_tag="PXA33_001496"
     CDS             complement(4962..5252)
                     /locus_tag="PXA33_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5120819.1"
                     /translation="MEQLQAVSNEQVFAKLCEVERLLKTKGVNEHSRELWDLGDVAAY
                     FGYTKEHTSRSVVSSPHFPKPIALDGLRGKGRGAKKWVSGEVVKFCLMWKVK"
     gene            complement(5227..5496)
                     /locus_tag="PXA33_001497"
     CDS             complement(5227..5496)
                     /locus_tag="PXA33_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5120820.1"
                     /translation="MKNKMGSKFIRVRVEHPQRKKEDIELVQDVVAYHIFNLFKFEES
                     GLRGFSQYDLEIDVKVKPVLEQKEMPVDVWIDDLGGKNGTITSGQ"
     gene            complement(5486..5818)
                     /locus_tag="PXA33_001498"
     CDS             complement(5486..5818)
                     /locus_tag="PXA33_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5120821.1"
                     /translation="MREWEKQMPFSANLHIRPTGKFEVKNVNIEQIIGERGKNYGNFD
                     DVATMSQQLKAVLLDTNTQLKPHQKEAGEMICLKLARIFAGCNPDYEDNWRDIAGYAV
                     LGGKLNEK"
     gene            complement(5829..6281)
                     /gene="ssb"
                     /locus_tag="PXA33_001499"
     CDS             complement(5829..6281)
                     /gene="ssb"
                     /locus_tag="PXA33_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDL5120822.1"
                     /translation="MNLVTLVGRLGQDPDIRTMQNGEKAAALSVATSEKWTDKQTGVK
                     KESTEWHRVVLYRRLAEIAELYVKKGHLVSIIGKIKTRKWTDSNGVERSITEIIAEQM
                     QMLSSGEKNTPNTPNKAENKPQPKQKNQDVMTADEQKDIPQFDDDIPF"
     gene            complement(6281..6613)
                     /locus_tag="PXA33_001500"
     CDS             complement(6281..6613)
                     /locus_tag="PXA33_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5120823.1"
                     /translation="MVKFIRLNLINYFIEKDEYGEPVRCRAFRYDEIIQTKFIEGFRE
                     GEIPADDYGFEDWNGSVLILNEEGRKYFENYLFDVKKNYCRARDNYVYVLETLDEIEM
                     KLGLLEAK"
     gene            complement(6626..8986)
                     /locus_tag="PXA33_001501"
     CDS             complement(6626..8986)
                     /locus_tag="PXA33_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HDL5120824.1"
                     /translation="MNYQNEILSATEAFAIGVSLPPVAGASACALELHSDRLYQAFKQ
                     PYTSMVQQELFSKDPIHEPLRQSYFERLPRALAEHFGRQYKQKLHYQSQYDTADWFKA
                     EMARKMPRIEAVISQYCDVFDFLKQSHQDLDFLAELDSNVYFGAGVDTQQAVQKIKQD
                     AEVKGIQLPMHLCRLENSSRYLKAHGIRPLAYQTEEQIKQLALGIAYRVRQIQQDNIE
                     QNMHKATDGDTAYSVLLECYRAMMGEVNKLKIDSPYQKKARKGRLTEEEITTGFLKMT
                     CEKWWTRKLSKIAEQMKEHLAIACGMVNMLSPYCSNARLKAFEAQRKANIDYLKSMII
                     ANIAEPEEQLSLFETWLKSASNPKIKRLELLTRMNGFERYADKQGHEGWFITLTAPSK
                     YHAMLSRTSSVNPKWNGASPAETQVYLVNTWAKIRAKLNREGVMAYGFRVAEPHADAT
                     PHWHLILFTRPEDMEKLRRVFLSYALEVDGTEAGAKKYRCKFKRIEKEKGSATGYLVK
                     YVSKNIDGFGMDGELSDEANIQAKENAARVGAWSSVWCIRQFQQLGNIPISLWRELRR
                     LGSVEQEDETLEKLRVIADSGEWDVFTEELGGALVKRADLVARITYTERKSETGEALY
                     TMYQEPSLKVSGIINIKNGVQINTRPKEWSIQLKPKNWEEQQLQKKLENATETERAEV
                     KRKYYEKLGFNEEAIALLGELAPPWTCVSNCTGSKNNQISEEARNCLRNELITMRGRV
                     TEHQIDDLLNGKRLKIWGNSQKTMFVSYSRGRLIEHIVDNYESFLN"
     gene            complement(8983..9315)
                     /locus_tag="PXA33_001502"
     CDS             complement(8983..9315)
                     /locus_tag="PXA33_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5120825.1"
                     /translation="MSQITKSAYTHIHPNWQAPRGNTYRIERYELKRRYALFCNGERV
                     VLYKDPTHPNKNVESHIESDVLLLWLLKNELGWSGNIKEPLSPQDVENIRLLEQAFCL
                     TYNGTFIK"
     gene            complement(9312..9554)
                     /locus_tag="PXA33_001503"
     CDS             complement(9312..9554)
                     /locus_tag="PXA33_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5120826.1"
                     /translation="MSLITVNDYKIKKDILDADGKPCYQLYQGEELLRDTTNLSEIIA
                     YMFIRAEDKPFDELYNHIINQEAGAKTDIKFTVTVR"
     gene            complement(9706..9999)
                     /locus_tag="PXA33_001504"
     CDS             complement(9706..9999)
                     /locus_tag="PXA33_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5120827.1"
                     /translation="MDFVATYQKIRINTNHKQFTCNHLGEIWGKENDDFKRFLLRVAF
                     LQDNPEPKPWGAYSPQEKRLLHSFSLWLEQIADFQRVMKRESKLREKHINAPD"
     gene            complement(10012..10344)
                     /locus_tag="PXA33_001505"
     CDS             complement(10012..10344)
                     /locus_tag="PXA33_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5120828.1"
                     /translation="MSKQLYYRHKSRWNRLNRLKKMNKRRLNLFLQEKRVANLESRLA
                     DEVELAKLNSEQIGDLLQALELKVNRLIRQNEKLKARVIKLEKVKQTQKQGLFAMFKK
                     VLGSKGNP"
     gene            complement(10427..10699)
                     /locus_tag="PXA33_001506"
     CDS             complement(10427..10699)
                     /locus_tag="PXA33_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5120829.1"
                     /translation="MNLDHTCTNCKSKNLRVRTSEKIGLLTIQAVTYCNNCGTEQLVQ
                     SQIIRVRTPIYKERPEAMRINKCLMEADLNTPDLFDGVVADQPKQD"
     gene            10832..11077
                     /locus_tag="PXA33_001507"
     CDS             10832..11077
                     /locus_tag="PXA33_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5120830.1"
                     /translation="MNTEDQIIELTVEVENLRHQLNAKNIILDDLKGRLTDYQRKRLD
                     RLFERSRRQYLNDLDLEEEQAEALEAICNELESFLAN"
     gene            complement(11176..>11382)
                     /locus_tag="PXA33_001508"
     CDS             complement(11176..>11382)
                     /locus_tag="PXA33_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL5120831.1"
                     /translation="NKKIQKAIELVGTQTKLAEACGVSQPTVLKWLNGGGISAEYLLK
                     IEAATNGQVTIREICEELGNATAK"
BASE COUNT         3327 a         2639 c         2025 g         3391 t
ORIGIN      
        1 tttcaaaaca atcccctttt agcccaactc aaacaacaaa ttgaagcaag caaagaatat
       61 gtcgcaggcg ttgtcaaagc ctcagacaaa gcattcggtt ttttagaatg cgataaaaaa
      121 agctatttta ttccacctgc ggaaatgaaa aaagtaatgc acggtgattc agtgaaagcc
      181 gtagtcaaac gtgatggcga caaagaacag gtagaaattg attcactact tgagccaatg
      241 ctggatagat ttattgccca agttcgcttg aataaagagg gcaaattgca acttgcggtt
      301 gatcacccaa gcattaagca aactatcccg gcaaataccc ataaaaaagc cactgaaaaa
      361 ttagaaaatg gcgactgggt ggttgcacaa ttaaaaacgc acccattgcg tgacgaccgt
      421 tttttctttg ctcaagttac ccaatttatc tgtaaagaat ctgataattt tgccccttgg
      481 tgggtcacac ttgcccgcca cgaacagcct cgtgaaccgg tcgcaaatga aaaaaactat
      541 gaattacacg atgaattagc tcgtgaagat ttatctcatc tttactttac cactattgac
      601 agcccaagca cgcaagatat ggacgatgct ctgttcatcg aaccaattaa gcaaaatggc
      661 aagcgaaccg gctggcgttt agtggtagcg attgctgacc caaccgctta tatcccagaa
      721 aattcccata ttgaaaaagc cgctcgccag cgttgtttca ccaactattt gccgggcttt
      781 aacattccaa tgttacctcg agaactctca gacgatctct gttcgcttgt cccgaatgaa
      841 aaacgccctg ccctcgttgg ctttattgaa accgatttaa acggcaatct cattaaagag
      901 acaaccttta cctctgcttg ggtcgaatca aaagatcgct tggcttacga cgatgtttcc
      961 gattatctcg aaggcgtgga aaatgcgtgg caacctcagt ctgaagaaac aaaacaacaa
     1021 attgagtggc tacatcaatt tactcaagcc cgcattcaat ggcgtgccga gaatgcgttg
     1081 ctattcaaag aacaaggcga ttataccttt gagttaaatg aagatggctc ggtaaaagat
     1141 attcacgttg aatatcgccg tattgcgaat caaatgatcg aagaatcaat gattcttgcc
     1201 aatatttgtt gtgcaaaatt cttaagccaa catgccaaaa ccggtgtatt taatacccat
     1261 tcaggttttg atccgaaaaa ttatgaattg gtaaaatcat ttttactcca aactttagcc
     1321 agcgatgaaa atcgtgatga gcttgccgaa cgctactccg ccgagcgttt agcaacctta
     1381 gaaggctatt gccaaatgca acgtgatatt caagactacc cagagaaatt cttagagttg
     1441 cgtttacgac gttatttaac cttcgctgaa tttaaagccg aagttgcccc gcatttcgga
     1501 ctaggcatta ccgattacgc cacttggaca tcacccatcc gtaaatatgg cgatatggta
     1561 aaccaccgtt taatcaagca agtgttactt ggcaaacaaa gccaagcggt ggaagaatca
     1621 gttttaagcc gtttacaaga agctcgccgt caaaaccgtt tagtagaacg tgatattgct
     1681 gactggcttt atgctcgtta ccttgcccca atggtggaac aagcggtcga atttgagtgc
     1741 gaaattgcag atgtttcccg tggtggcttg cgtgcgaaag tcataaaaaa cggtgctcaa
     1801 atttttgtgc cattctccag cttgcacgac aacaaagagg aaatggaata ccgccaagaa
     1861 gaacttgcac tttatattaa aggtgaaaaa gcctatcaga tcgggcaagc ggtgaaagtg
     1921 aaactcaccg aagtacgctt agaaacccgc tcaatcgtag gaaatatcat ttaaatttca
     1981 agcggttata ttttgatgag attttgcaaa ttttctctaa aatataaccg cttgtttata
     2041 actttagggg aacaaaggag aaacaatggc aacttatatt gtaggcgacc tacacggttg
     2101 ctttaacgaa tttcagcttt tattagaaaa agtcaattat aaccccaagc aagacgaact
     2161 ctttttaaca ggggatttag tcgcccgtgg tgaggactct ttagcctgtt tacgttttgt
     2221 gaaagactct gcaaacaatg ccaaaaccgt gctaggcaac cacgatttgc acttgcttgg
     2281 catcaaacgg gtaaaaccaa atgataagat tgatgctatt tttgaggcta aagatcggct
     2341 cgatttgcaa aattggctca gaaatcaacc gcttgtaatt catcacccca aacatcaatt
     2401 tttgctgact cacgcaggaa tcagccctga gtgggattta gcaactaccc tagcttgtgc
     2461 caaagaggca gaaaaggtgc tacaaagtga caattatgcg gactacatcg cccaaatgta
     2521 tgagaataag cccgaacgct ggagtgaaga tttgcaaggt gttgaacgtt ggcgttatat
     2581 catcaatgtg tttacccgta tgcggttttg ctacaccgac aagcggttag attttgactg
     2641 taaattaccg cttgaacaag cccctcaaga actgctggct tggtttgact tagataatcc
     2701 gttatttaca cagaaaaaca ttatctttgg tcactgggca agcctaatgg gcaaatgcac
     2761 tcgtccaaac atctatgcac ttgataccgg ctgtgcttgg ggaagccatt taaccatgct
     2821 ccgttgggaa gataaacaaa tttttaccca aaagcgtcta aaataaaagc attcgaccaa
     2881 aagcatattg cactaagttc ctttttccaa tagaatgcac accgttaatg cgactatagc
     2941 tcagttggtt agagcaccac cttgacatgg tgggggtcac tggttcgagt ccagctagtc
     3001 gcaccattat ttttaagcct gaatatcccc attcaggctt aaccccaaaa atacccctta
     3061 taaatcaata gcttattgat ttaaaacact tccccaagtg tagcataaca tcaataaaaa
     3121 catcattttt gatatgaaaa cttgatattt attgatcgtt tatgatctct attaacattc
     3181 ctttgcacac atttgcacac ttaaactgca cacatacaca aaggaataaa aaaatggcta
     3241 ctattattaa gaacggcaac aaataccgag cacaagtccg caaaaaaggc atttataaaa
     3301 ctgccacttt cagcaccaaa acagaggcaa accgctgggc ttacgcttta gaggctcaaa
     3361 ttgatgccgg agaatataac accaccccga acattctttt tgccgaatta atcgacaaat
     3421 acctcaaaga ggtcactccg accaaacgtg gcaaaaggga agaacgctat cgcctgctaa
     3481 gaattgccgg cagcaacttg ggaaatatac cattaccggc actcaccaaa gagcatttac
     3541 gccaatggca aaaccaacgc ttgcaagaag tgcagccggt ttccgtctca agagaaagag
     3601 ccactttatc ggcactcttt acaaaagcaa tggaatggga ttatctcaaa gaaaatcctc
     3661 taaaaacgct ggaaaaaatc aaaacgccac cagcaagaac tcgccgatat agcgatgagg
     3721 agatagacaa actgattttt gtctccggct acagccctaa ccagccaccg ctattaatgc
     3781 aacaaagagc cggagcagca ctattatttg cgattgagac tgcaatgcga gcgggtgaaa
     3841 ttgctggttt aaaatgggaa catataaacc tgcaggcacg caccgcattt ttgccacaaa
     3901 ccaaaaacgg ttattcacgc accgtgccac tctcaaccaa agcaatagca attttaaatc
     3961 agttggcaca acttgaaccg aacaagcaaa acagtgtttt ccagctcacc agtcgcagcc
     4021 ttgattcgaa ttttcgcaag ctgaaacaaa aagccaatct acacaatgcc gatctgcatt
     4081 ttcacgacac acggcgagag gcactcacac gtttgtctaa atatctgacg gtaatggagc
     4141 ttgccaaagt atcggggcat cgagatttaa gtattttgca aaatacctac tataacccgg
     4201 atatttcaga acttgtgagc aaactgcaat aaaaacaaaa gccgataaac gaaaatttat
     4261 cggctttttt gtatttatta cttgcaatgt aggataccct acattataat aacctcactt
     4321 tcaagagaca gcttgaaagt gagcgtaagg cttaaacctt acgaaatgaa gaaggatagc
     4381 acaatgttta aatacattat cctagttgtc ttcttattag tccttagctc cccagcttac
     4441 taatctgaag caactcgctg ggggtgaaag ctcccagctc ttcaacaaaa tttataatag
     4501 ggataaatta tggcaatgtc aagaaatgaa atccaagcta aaagcgaggc aaaacgagga
     4561 ataaagcaaa aatcctttaa actaccgctt gaggtgatcg cagaaatcga agtacttagc
     4621 caaaaactca acattccaca aaaccagctg attattcaag ctattcaaca attcaagcaa
     4681 aacaacccat aaaacaaaag ccgataaata atgaaattta tcggcttttg tattttgaaa
     4741 tactttacaa ataatcgtta ttatccatct ttacaaatgc cagccaatgc gttttgttat
     4801 gtttaccgct tttatgccca tagataggtt ggtattcgat aacatttaaa attttcgata
     4861 cagggattcc cgattgtcag caaacaatac cgaggggttg tgcttattaa agtgaaacat
     4921 tcggctaccg caacaagcat ctaaaattat cttttttatc atcacttcac cttccacatc
     4981 aaacaaaatt tcaccacttc accggatacc cacttcttcg cccccctgcc cttgcctcgt
     5041 aagccgtcaa gggcaatcgg tttcggaaaa tgtggactag atacaacact tcggctggtg
     5101 tgttctttag tgtagccgaa atatgctgct acatcgccca aatcccacaa ttcacggcta
     5161 tgctcattca cccctttggt ttttaataac cgctcaacct cacaaagttt tgcaaaaact
     5221 tgctcattac tgaccgcttg taattgttcc atttttacca cctaaatcat caatccaaac
     5281 atctacaggc atttcttttt gttctaacac tggcttaact ttcacatcaa tttccaaatc
     5341 atactgacta aacccccgta acccactttc ctcaaattta aaaagattaa aaatatgata
     5401 agcaaccaca tcctgcacaa gctcaatatc ttcttttttc cgctgtggat gctcaactct
     5461 gactcgtata aatttactcc ccattttatt tttcatttaa tttccctccc agcaccgcat
     5521 aacccgcaat atctcgccag ttatcttcat agtccggatt acaacctgca aaaatgcgag
     5581 ccagtttcaa gcagatcatc tcgcctgcct ctttttgatg tggtttaagt tgagtattgg
     5641 tatccagcaa cactgctttt aactgctggc tcattgtggc aacatcatca aagttgccgt
     5701 aatttttgcc acgttctccg attatttgtt ctatgtttac atttttcact tcaaattttc
     5761 ctgtaggtct tatatgtaaa tttgcactaa atggcatttg tttttcccat tctctcatta
     5821 ttcaccccct aaaacggaat atcatcatca aactgcggaa tatctttctg ctcgtccgca
     5881 gtcatcacgt cttgattttt ctgctttggt tgcggtttat tttctgcctt atttggcgta
     5941 tttggcgtat ttttctcacc actactgagc atttgcatct gctcggcaat aatctctgta
     6001 atactccgct caacaccgtt gctatccgtc cattttcggg ttttaatttt cccgataatt
     6061 gataccaaat gccctttttt tacatacaat tcggcgattt cagctaatcg gcgataaagc
     6121 accaccctat gccattcggt gctttctttc ttcacacctg tttgcttatc tgtccacttt
     6181 tcagaggtcg ctaccgataa agccgcagct ttctcaccat tttgcattgt tctaatatca
     6241 gggtcttgcc ccaaacgtcc gactaatgtg actaagttca ttatttcgcc tccaataatc
     6301 ctaacttcat ttcaatttcg tctaaagttt ccaagacata tacatagtta tcccttgctc
     6361 tacaataatt cttctttaca tcaaaaagat agttctcaaa atatttacgt ccttcttcat
     6421 ttaaaatcag aaccgaacca ttccaatctt caaatccata atcatcagct ggtatctctc
     6481 cctctcgaaa tccttcaata aacttagttt gaataatctc atcataacga aacgctctgc
     6541 aacgcaccgg ttctccatat tcatctttct caatgaaata attaatcaaa tttaaacgaa
     6601 taaatttaac cattttctta atatcctaat tcaaaaaact ctcataatta tcaacaatat
     6661 gctcaattaa acgcccacgg ctataactca caaacattgt tttttgcgaa tttccccaaa
     6721 tttttaaccg cttgccgttt agtaaatcgt ctatttgatg ttcagtaacc cgtcctcgca
     6781 ttgtaatcag ttcatttctt aggcaatttc gagcctcttc actaatctga ttattttttg
     6841 accccgtaca gttactgaca caagtccaag ggggagcaag ctcccctaat aaagcgatag
     6901 cctcttcgtt aaaccctaac ttctcgtagt atttacgttt aacctccgct cgttccgttt
     6961 cggttgcatt ttctaatttt ttctgtaatt gttgctcctc ccagttttta ggtttaagtt
     7021 gaatgctcca ctctttcggg cgtgtattaa tctgcacacc gtttttaatg ttaataatgc
     7081 cgctcacttt taatgatggc tcttgataca tcgtatataa cgcctcaccg gtttcgcttt
     7141 tgcgttcggt ataagtaata cgtgcaacta aatcggcacg tttaaccaac gcaccgccta
     7201 attcttcggt gaaaacgtcc cactcaccgc tatctgcaat cacacggagt ttctctaaag
     7261 tttcatcttc ttgctctaca ctccccaaac ggcgtaactc acgccataag ctgatcggaa
     7321 tattacctaa ctgctggaac tgtcgaatac accaaacgct agaccacgct cctacacgag
     7381 cggcattctc tttcgcctga atatttgcct cgtctgaaag ctcgccgtcc attccaaaac
     7441 cgtcaatgtt tttagaaaca tatttcacta aatagcccgt tgccgagcct ttctctttct
     7501 caatacgctt gaatttgcaa cggtattttt tcgcaccggc ttctgtgcca tcgacctcta
     7561 aggcataact caagaacacg cggcgaagct tttccatatc ttccggacga gtgaatagaa
     7621 ttaaatgcca gtgtggggta gcgtcagcgt gaggttcagc cactcgaaaa ccgtaagcca
     7681 ttacgccctc acggttgagt ttggcacgga ttttcgccca agtattcaca aggtaaacct
     7741 gtgtttccgc agggcttgct ccattccatt tcgggttcac gctactggtt cttgaaagca
     7801 ttgcgtgata cttagacgga gcagtcaagg taataaacca cccctcgtgc ccttgcttat
     7861 ctgcataacg ctcaaatcca ttcatacgag tgagtaattc aaggcgttta attttcgggt
     7921 tagaggcaga cttcaaccac gtttcaaata atgaaagttg ctcttccggc tcggcgatat
     7981 tggcaatgat cattgatttc aaataatcaa tattcgcctt gcgttgagcc tcaaacgctt
     8041 tcaagcgagc atttgaacag taaggcgaaa gcatattcac cataccgcac gcaatagcta
     8101 aatgctcttt catctgttcg gcaattttag ataactttct agtccaccat ttttcacagg
     8161 tcatttttaa aaagcctgtg gtaatttcct cttcagttaa tctacccttt ctcgcttttt
     8221 tctgataagg cgaatcaatc ttgagcttat ttacctctcc catcattgcc cgatagcatt
     8281 caagcaacac actataggct gtgtcgccat ctgtcgcttt gtgcatattc tgctcgatgt
     8341 tatcttgctg aatttggcga acacgataag caatacctaa ggcaagctgt ttgatttgct
     8401 cttcggtttg gtaagcaagc gggcgaatgc cgtgagcttt taaatagcga gagctattct
     8461 ctaaacggca aaggtgcata ggcagttgaa tacctttcac ttcggcatct tgtttaattt
     8521 tttgtaccgc ttgttgcgta tcaacaccgg caccgaaata aacattacta tctaattcag
     8581 ctaaaaaatc taaatcttga tgggattgct tcagaaaatc gaatacatcg caatactggc
     8641 taataactgc ctcaatgcgt ggcattttgc gtgccatttc cgctttgaac cagtctgctg
     8701 tgtcgtattg gctttggtaa tgtagctttt gcttatactg tctgccgaaa tgctctgcta
     8761 aggcacgagg caaacgctca aaataagatt ggcgtaacgg ttcgtgaata gggtctttcg
     8821 agaataattc ttgctggacc attgaagtgt aaggctgttt gaacgcttga tataaacgat
     8881 cagaatgaag ctctaatgca caagcagaag caccggcaac aggtgggagt gaaacaccta
     8941 ttgcgaaagc ctctgtagca gatagaatct cattctgata attcatttaa tgaaagttcc
     9001 gttataagtt aaacaaaatg cttgctctaa caagcggata ttctcaacat cttgagggct
     9061 taacggctct ttaatattac cgctccaccc taattcattt ttcagtagcc ataacaacaa
     9121 aacatcactt tctatatggc tctctacgtt cttattgggg tgtgttgggt ctttatataa
     9181 aactaccctc tcaccattac aaaataaggc ataccgccgt tttagctcat atcgctcaat
     9241 ccggtaagtg ttacctctcg gggcttgcca gtttgggtga atatgtgtgt aagcggattt
     9301 agtaatctgg ctcatctcac cgttaccgta aatttaatat ctgttttagc tcctgcttct
     9361 tggttgatga tatggttata caactcatca aacggtttat cttcggcacg aataaacata
     9421 taagcgatga tttcgcttaa attggtggta tcacgcaata gctcttcgcc ttgatacaac
     9481 tgataacacg gcttgccgtc tgcatctaaa atatctttct tgattttgta atcgttcaca
     9541 gtaattaatg acatttttct accctctact cattcactaa acattcgttt tgataacgct
     9601 gaaaaaacac ttgataccgg cgataagcat agcgattacc ggttttgcac cacagtttgt
     9661 gaaaaaactc acacaattca cggttacggc tcatatttac tccttctaat ccggtgcgtt
     9721 aatatgtttt tcacgcagtt ttgattctct tttcattact cgctgaaaat ctgctatctg
     9781 ctctaaccac aagctaaaac tatgtaaaag gcgtttttct tgtggggaat acgctcccca
     9841 aggtttcggc tcggggttat cctgcaagaa tgcgactcgt agtaagaaac gtttaaaatc
     9901 gtcgttttcc ttcccccaaa tctcgcctaa gtgattgcaa gtaaattgct tgtgattagt
     9961 gttaatgcgt atcttttgat acgttgccac gaaatccata tctacctctt actaaggatt
    10021 acccttgcta cccaacacct ttttaaacat tgcaaacaaa ccttgctttt gggtctgttt
    10081 gactttttcc agcttaatca cacgggcttt cagcttttca ttttggcgaa ttaaccgatt
    10141 cacttttaac tccaacgctt gcagtaaatc gccaatctgc tcactattca gctttgccag
    10201 ctctacttca tcagctaacc ggctttctaa attcgccacc cgcttttctt gcaaaaacag
    10261 attaaggcga cgtttattca tcttcttaag gcggttaagt ctgttccaac gtgatttgtg
    10321 tcggtaatat aattgcttac tcattgttca aattcctgaa tttagggtgc aaaaatctcg
    10381 ccctatgcaa ataaggctta ttttgacgat tgtttaaaaa ttgggattag tcttgcttag
    10441 gttggtctgc cacaacgcca tcgaacaaat ccggcgtgtt caaatcggct tccatcaagc
    10501 atttattaat acgcattgct tccggtcgct ctttataaat cggtgtgcga acacggataa
    10561 tttgactttg cacaagttgc tctgtgccgc agttattaca ataagtaaca gcctgaatag
    10621 tcagcaaacc gattttttca gatgtacgaa cccgtaaatt tttacttttg caatttgtac
    10681 aggtgtggtc taaattcatt atttccccta cgccttcccc aaggctctat taacattctt
    10741 tacgttgatt tcggcttatc tatccccata gacaagcagt cgaattttgg ttatgtttta
    10801 gatattgttt aaccctccaa aaggaaattt tatgaatact gaagatcaaa ttattgaact
    10861 gactgttgaa gttgaaaact tacgccatca actaaacgcc aaaaacatta ttcttgatga
    10921 tttaaaaggt cgcttaacag actaccaacg caaacgactg gaccggcttt tcgaacgttc
    10981 acgccgacag tatttaaatg accttgacct tgaagaagag caagctgaag ctcttgaagc
    11041 aatttgcaac gagcttgagt cgtttctggc gaactaatac caaaatccaa caaggtcaaa
    11101 tctaaacgcc atttaccgtc ttgattacgt cttacagtaa atttcccact cgctagccct
    11161 gcctgttcag ggcttttatt ttgctgttgc atttcccaac tcctcacaaa tttcacgaat
    11221 tgttacctgc ccgtttgtgg cagcttcgat ttttagtaga tattcagcgg aaattccgcc
    11281 tccgtttagc catttcaaga ccgtaggctg actaactcca caagcttcag ctaattttgt
    11341 ttgtgttcct acaagttcaa tagctttctg aattttctta tt
//
LOCUS       DANLRK010000080        11269 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.080, whole genome shotgun sequence.
ACCESSION   DANLRK010000080 DANLRK010000000
VERSION     DANLRK010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 11269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11269
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.080"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(145..492)
                     /gene="frdD"
                     /locus_tag="PXA33_001509"
     CDS             complement(145..492)
                     /gene="frdD"
                     /locus_tag="PXA33_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HDL5120832.1"
                     /translation="MSLDQNPKRSNEPPVWLLFSAGGMVSALFFPVVIFIIGLLLPFG
                     LVSPDNIISFAHTWIGKLAIMVLAIFPMWAGMHRIHHGLHDLKVHTPAGNVIFYGLSI
                     LYTILVIIAVVQI"
     gene            complement(506..898)
                     /gene="frdC"
                     /locus_tag="PXA33_001510"
     CDS             complement(506..898)
                     /gene="frdC"
                     /locus_tag="PXA33_001510"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HDL5120833.1"
                     /translation="MSATKRRPYVRGMKASWWKKLDFYKMYMVREATCLPTVWFCIVL
                     FYGAVALSKGTFTTDFVDFLQNPLVVILNIISLAAMLYHAATLFVMTPAVMSVMVKGK
                     HLPVSVGRNILWAATGVISVIALILVYI"
     gene            complement(908..1639)
                     /locus_tag="PXA33_001511"
     CDS             complement(908..1639)
                     /locus_tag="PXA33_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005639269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HDL5120834.1"
                     /translation="MANLDKMTIEVLRYNPESDSEPHLDRYEVPYDSQTSLLDALGFI
                     KDELEPELSYRWSCRMAICGSCGMMVNGKPKLACKTFLRDYSGFMRIEPLANFPIERD
                     LVVDLSHFIDSIEAIKPYIIDNKAPEGQRTKQTPAQLEKYRQFSMCINCGLCYAACPQ
                     FGLNPEFIGPAAITLAHRYNLDNRDNGREERMKLLSSKNGVWSCTFVGYCSEVCPKHV
                     GPASAVNQGKLESAKDYVISMIKPR"
     gene            complement(1655..3457)
                     /gene="frdA"
                     /locus_tag="PXA33_001512"
     CDS             complement(1655..3457)
                     /gene="frdA"
                     /locus_tag="PXA33_001512"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010944100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HDL5120835.1"
                     /translation="MQSVNFDVAIIGAGGGGLRAAIAAAEANPNLKIALISKVYPMRS
                     HTVAAEGGSAAVIKDTDSFDNHFNDTVGGGDWLCEQDIVEYFVEHSPIEMTQLERWGC
                     PWSRREDGEVNVRRFGGMKIERTWFAADKTGFHILHTLFQTSIKYPNIVRFDEHFVLD
                     ILTDNGEARGCVAMNMMEGTLVQINANAVVIATGGGCRTYRFNTNGGIVTGDGLSMAY
                     RHGVALRDMEFVQYHPTGLPNTGILMTEGCRGEGGILVNKDGYRYLQDYGLGPETPIG
                     KPENKYMELGPRDKVSQAFWQEWRKGNTLKTAKGVDVVHLDLRHLGEKYLLERLPFIC
                     ELAKAYEGVDPAKAPIPVRPVVHYTMGGIEVDMNAETSIKGLFAVGECASSGLHGANR
                     LGSNSLAELVVFGKVAGENAARRALEVTARNQPQIDAQAKDVVARLHALANQEGNESW
                     SDIRNQMGDAMEEGCGIYRTQESMEATVNKIQELKERYKNISVKDKSSVFNTDLLYKI
                     ELGFILDVAQSIACSAVERKESRGAHQRLDYVERDDVNYLKHTQAYYNAEGTPTIKYS
                     DVKITKSQPAKRVYGAEAEAQEKVKKAAEAQAQQ"
     gene            3790..4773
                     /gene="epmA"
                     /locus_tag="PXA33_001513"
     CDS             3790..4773
                     /gene="epmA"
                     /locus_tag="PXA33_001513"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HDL5120836.1"
                     /translation="MNNLQLDNIDWQPTASIPHLIQRSKIITEIRKFFTDRGLLEVET
                     PILSEFSVTDVHLSTFNTQFQSPFSPEAKTLHLMTSPEYHMKRLLAAGSGPIFQLCKV
                     FRNEEAGKRHNPEFTMLEWYRPHFDMYRLINEVDDLLQQILDCEPAESFSYQFVFQTY
                     VGLDPLSATKAQLVEKARKHGLQCDDDEQRDTLLQFLFSEVVEANIGQERPTAVYHFP
                     STQAALAQISSEDHRVAERFEFYYKGLELCNGFHELDDADEQIHRFEQDNLQREKMGL
                     EPQQLDVRFLAALKAGFPNCSGVALGVDRLLMLAMDAEKIEEVISFGIEKA"
     gene            4855..5745
                     /gene="rarD"
                     /locus_tag="PXA33_001514"
     CDS             4855..5745
                     /gene="rarD"
                     /locus_tag="PXA33_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HDL5120837.1"
                     /translation="MNISGKLQGWHYALLCYLTWGTFPIYWYPINQSGMPAEQILAQR
                     VLWSALFAVFLLLIFQQGRAVINAFKQPKVLGLFVLSSFMIGVNWLVYLWAIVNEHIL
                     DASLGYFINPIFNVFVGRLILKEQLNKAQLLALLFAISGILWLAIPAGQIPWVALILA
                     ASFCIYGLIRKLAPMEPLSGLALETLLMSPFAITYLCFCYHQNILVFSELNPLQMAVL
                     LCSGIATTLPLLWFAMGARRISMSLLGMLQYISPTLQFLCGVLIFNETLSAERAVGYI
                     LVWIGVGVFLLGMKKAGVKR"
     gene            complement(5756..6367)
                     /locus_tag="PXA33_001515"
     CDS             complement(5756..6367)
                     /locus_tag="PXA33_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL5120838.1"
                     /translation="MKYEAKPLIISPNHIQQRLHGKLLARALLIIALLMLAKFIYIAS
                     LSNYFTFYLMEKFQLNIAMAQLSLFTFLGAVALGAFVGGPIGDKIGRNAVIWFSFLGM
                     APFALLLPHADLFWTIVCAIFAGFIMSSAFSAMVVYAQEAVPGRVGLISGTMFGLMFG
                     IGGLSAAVLGFFADEYGLESIFKACAYLPLLGFAALGLPKRNI"
     gene            complement(6395..6930)
                     /locus_tag="PXA33_001516"
                     /pseudo
     CDS             complement(6395..6930)
                     /locus_tag="PXA33_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251153.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            7122..9131
                     /locus_tag="PXA33_001517"
     CDS             7122..9131
                     /locus_tag="PXA33_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HDL5120839.1"
                     /translation="MDLIKKLNTGSTFRVPIFLPSLLFVSFVAVFCIIFPQQAQTSLD
                     TIKNSLFQHFSWFYIFAGSIFFLFLIFLSFSRLGDIKLGADTDEPEFGFGSWIAMLFA
                     AGMGIGLMYFGVAEPILHYLKPVQQNLTEPERMKEAMMTTFYHWGIHAWAIYGVIALA
                     LAYFGFRYKLALTIRSGFYPLLKHRISGFWGHLIDIIALCSTIFGLTTTLGFGVMQVS
                     AGFNNLGLIEQSNFTVLAIIVTVAMALAVLSAVSGVGKGVKILSEINLTLAGLLLIFV
                     IITGPTLLLFSSFTENLGYYFSSLLEMSFRTFAYEPEHQGWLSGWTVLYWAWWASWAP
                     FVGLFIAKISKGRTIREFILGVLFVPSLFNILWMTSFGSSAIWFDQQTAGALAEVSGN
                     TEQLLFTFFEQLPFGSIASFVAVIVISIFFITSADSGIFVLNSIASQGEENAPKWQSV
                     LWGALLAILALSLLYSGGLASLQTMTLIIALPFTFIMLILCIGLWKGLMVDNQYFNKK
                     FSQGSQHWAGKDWKQRLEKIINPSNKQDVRHFFIKVARPAFLELIEEFESYGLIAKMN
                     FTNEQNPKLEFEVVKENLRNFIYGIESVPRELSDLVVGDDNLPNIEQNTIYEPITYFL
                     DGRKGYDVQYMTKEELIADVLQQYERFINLAMDNSHDLMTADFNH"
     gene            complement(9196..9663)
                     /locus_tag="PXA33_001518"
     CDS             complement(9196..9663)
                     /locus_tag="PXA33_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HDL5120840.1"
                     /translation="MKSLKLKIPPPAWFLLCAALMWLVAQYVPVSLPNYQHTAIFFSG
                     LAVAIIGGAVAVSALWVIHQAKTTYSPFRPQHTAKLVTWGIYRKSRNPMYLSLLLGLI
                     AWALWLGAMLAWLVLPLFVWLITYFQIKPEEQILAQKFGTHYAKYCNQVRRWL"
     gene            complement(9665..11239)
                     /gene="murJ"
                     /locus_tag="PXA33_001519"
     CDS             complement(9665..11239)
                     /gene="murJ"
                     /locus_tag="PXA33_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HDL5120841.1"
                     /translation="MSKKLLRSGMIVSFMTLISRILGLIRDIVVATILGTGVSADIFL
                     FANRIPNFLRRLFAEGAFSKAFVPVLAEYNADNDPNKTREFIAKVSGTLGGLVTVVTL
                     VAMIASPVVAALFGTGWFLDWLYDGPNAEKFTQASFLLKITFPYLWFITFVALSGAVL
                     NTIGKFGVMAFSPVLLNVAIIAVALWGRDYFDSPDIALAWGVFLGGLLQFLFQIPFMK
                     KEGLLVKPKWAWKDEGVTKVRKLMIPALFGVSVTQLNLLLNQVIASFLITGSISWMYY
                     ADRLIEFPLGLFGIAISTVVLPSLSRIAKNKELTEIQRGEHFQNTMDWGVRMVLLLGI
                     PAMIGMAVLAQPIIMTMFMRGKFGFEDVLATSYPLWVMCLGLNSYMLISVLANGFYAN
                     QNTKTPVKVGIIAALSNICFGLAFAPFLGYIGLALASACSALVNVSLLYVNLSKNGYY
                     KVSHKTVLFVLKLFIAACVMGALVAYFTPEIELWALMTMWQKVYWLIWLMILAGGSYF
                     ASILVLGIRKKDFRSA"
BASE COUNT         3304 a         2524 c         2197 g         3244 t
ORIGIN      
        1 atcttttttg cagatattta atacaaaacg atcgttttat cggcaaaatt tctaatattt
       61 gcaaattttc ttataaattc gaccgcttgt tagcatatta aaaatgaaaa gaccatctgc
      121 tttatacaag atggtctttc tcatttaaat ctgcacgact gcgatgatta ctagaattgt
      181 gtataagata gacaatccgt agaaaatcac attacctgct ggcgtgtgaa ctttcaggtc
      241 gtgtaatcca tgatggatac ggtgcattcc tgcccacata gggaaaatcg ctaataccat
      301 aatagcgagc ttgccgatcc aagtatgggc aaaggaaatg atattatctg gtgatactaa
      361 cccgaatggc agtaataaac cgataataaa gattaccaca gggaaaaata atgcacttac
      421 cattccccca gcactaaaaa gcaaccatac cggcggttca tttgaacgtt ttgggttttg
      481 atcaagactc attttcttcc ccttcttaaa tataaaccaa gattaatgca attacgctaa
      541 ttacgcctgt tgctgcccaa agaatatttc ttccaaccga gactggtaag tgtttacctt
      601 taaccatcac ggacatgacg gcaggtgtca tcacaaaaag tgttgccgca tgatataaca
      661 ttgctgccaa tgaaattatg tttaggataa caactaacgg attttgtaag aaatccacaa
      721 aatcggtagt aaatgttcct ttacttaacg caactgctcc ataaaataat acaatgcaga
      781 accaaacagt tggaagacag gtagcttcac gcaccatata cattttatag aagtcgagtt
      841 ttttccacca gctggctttc attcccctta cataaggtct acgttttgtt gctgacattt
      901 cttcctccta tctcggtttg atcattgaga taacataatc tttcgcactt tcaagtttac
      961 cttggtttac agccgaagca gggccgacgt gtttcggaca gacttccgag cagtaaccca
     1021 cgaaagtaca gctccaaaca ccgtttttgc tgctgagtag cttcatacgc tcttcacgac
     1081 cattatcacg gttatccaag ttatagcggt gagccaatgt aatcgcagca ggtccgataa
     1141 attcagggtt caaaccaaat tgtgggcaag ccgcataaca taaaccgcag ttgatacaca
     1201 ttgagaattg acgatatttc tctaattgtg ccggcgtttg tttagtacgc tgtccttccg
     1261 gtgctttatt atcaataata taaggtttaa ttgcctcgat gctatcaata aagtggctta
     1321 aatccactac taagtcacgc tcaatcggga agttcgccag cggctcaatt cgcataaaac
     1381 cgctgtaatc acgcaggaat gttttacaag ctaacttcgg cttaccgttt accatcattc
     1441 cgcaagaacc acaaatcgcc atacggcaag accagcgata agaaagttcc ggttctaatt
     1501 cgtctttaat gaagcccaat gcatctaaaa gtgaagtttg gctgtcatac ggcacttcat
     1561 atctgtctaa atgtggctcg ctatctgatt cagggttgta gcgtagcact tcgatggtca
     1621 ttttatctaa atttgccatt ttactactcc ttctctattg ttgtgcttga gcttctgctg
     1681 cttttttgac tttttcttgg gcttcagcct ctgcaccata cacacgttta gccggttgtg
     1741 atttagtgat tttcacatcg ctgtatttaa tcgtcggtgt accctctgca ttgtagtacg
     1801 cttgtgtgtg ttttaagtag ttcacatcat cacgctcaac atagtctaaa cgttggtgag
     1861 ccccacgaga ttctttacgc tctaccgcag agcaagcaat ggattgagcc acatctaaaa
     1921 tgaagcctaa ttcaattttg tagagtaaat cagtgttaaa cacacttgat ttatctttta
     1981 cgctgatgtt tttataacgc tcttttagct cttggatttt gtttacagtc gcttccatac
     2041 tttcttgggt acggtaaata ccacaacctt cttccatcgc atcacccatt tggttacgga
     2101 tatccgacca agattcatta ccctcttggt tcgctaatgc gtgcaagcga gccaccacat
     2161 cttttgcttg tgcatcaatt tgtggctggt tgcgagcagt cacttctaac gcacggcgag
     2221 ccgcattttc accggcaact ttgccgaaca ccacaagctc tgctaatgag ttagaaccca
     2281 aacggttcgc accgtgcaag cctgatgaag cacattcacc tactgcgaat aagcctttaa
     2341 ttgaggtttc cgcattcata tccacttcaa taccacccat cgtgtagtga accaccgggc
     2401 gaacaggaat cggcgcttta gccggatcga ccccttcata ggctttcgct aattcacaaa
     2461 taaacggtaa acgctcaagt aaatattttt cacctaaatg gcgtaaatct aagtgaacta
     2521 cgtccacgcc ttttgcggtt tttaaggtgt tccctttacg ccattcttgc cagaatgctt
     2581 gtgaaacctt atcacgaggg cctaattcca tatatttgtt ttccggtttg ccgataggtg
     2641 tttccggtcc taaaccataa tcttgcaggt aacggtagcc atctttatta actaaaatac
     2701 caccttcacc acgacaacct tctgtcatca aaataccggt atttggcaaa cctgttgggt
     2761 gatattgtac gaactccata tcacgcaaag ccacaccgtg acgatacgcc attgataaac
     2821 cgtcgccggt tacaataccg ccgttagtgt taaagcgata agtacggcag ccgccaccgg
     2881 ttgcaatcac aactgcattt gcattgattt gaacaagtgt gccttccatc atattcattg
     2941 ccacacaacc acgagcttcg ccgttgtcgg tgagaatatc cagcacaaaa tgctcatcaa
     3001 aacgcacaat attcggatat ttaatcgaag tttggaacaa agtatgcaaa atgtggaaac
     3061 cggttttatc cgccgcaaac caagtacgct caattttcat tcctccaaaa cggcgaacat
     3121 tcacttcacc gtcttcacga cgactccaag ggcaacccca gcgttctaat tgtgtcattt
     3181 caatcggcga atgctctaca aaatactcca caatatcttg ctcacataac cagtcaccac
     3241 caccgacggt atcattaaag tgattgtcga aagagtcggt atctttaatt accgccgctg
     3301 aaccaccttc tgctgcaacc gtatggctac gcattgggta aacttttgaa attaaagcaa
     3361 tttttagatt tggatttgct tcagccgccg caatagcagc acgcaagcca ccaccaccgg
     3421 cacctataat cgcgacatca aaattaacac tttgcataat gcactccgaa agttaaataa
     3481 cttaattcat taaaaagtaa tcatattgtg ccactatttg agcaatgaga taacctattt
     3541 ttacaaattt gtaacataaa aaatgtaatt ttgtgatcta cctcaaaaaa ggtaataaag
     3601 cttcataaag gtattgagaa tggctcttaa ttggacagtg atagaaagaa aatttctaat
     3661 gtttaaaatg agaaaattta atttgttgca aaaaagttaa aaccctctct ttatttttct
     3721 tgatcggtaa ctagggcaac tcactttttt aggctaaaat agcctcacat tttcaagcgg
     3781 tcatttttta tgaacaattt acaactcgat aatattgatt ggcaaccaac agcctctatt
     3841 cctcacctga tccaacgttc aaaaatcatt acggaaatcc gcaagttctt taccgatcga
     3901 gggcttttag aggtagaaac cccgatttta agcgaatttt cggtgacaga cgttcatctt
     3961 tcgactttca atacgcagtt tcaatcgcct ttttcccctg aggcgaaaac actccatctg
     4021 atgaccagcc cggaatatca tatgaagcga ctacttgctg ctgggagtgg accgattttc
     4081 caactgtgta aagtatttcg caacgaggaa gccggtaagc gacacaaccc tgaatttacg
     4141 atgctggagt ggtatcgccc gcattttgat atgtatcgct taatcaatga agtggatgat
     4201 ttactgcaac aaattttgga ctgcgaaccg gcagaatcat tcagctatca atttgtgttc
     4261 caaacctatg tcggattaga tccgctttct gccaccaaag cacaacttgt ggaaaaagcc
     4321 cgaaaacacg gcttgcaatg tgatgatgat gaacagcgtg acaccttgtt acaattttta
     4381 ttcagcgagg ttgtcgaagc caatatcggg caagaaagac caactgccgt ttatcatttc
     4441 ccttccaccc aagcggcatt agctcaaatt agctcggaag atcaccgtgt tgccgaacgt
     4501 tttgagtttt actacaaagg cttggaactc tgtaacggct tccacgaatt agacgatgct
     4561 gatgagcaaa ttcatcgctt cgagcaagat aatctgcaac gtgaaaaaat ggggctggag
     4621 ccacagcaac tagatgtgcg ttttcttgcc gcactcaaag cgggttttcc aaactgctca
     4681 ggtgtcgccc ttggggttga taggctgtta atgctggcaa tggatgcgga aaaaattgaa
     4741 gaggttattt ctttcggcat tgaaaaagcc taatttttta tgccgttttg tgatttgcaa
     4801 tttgcaaaaa aatcgacaaa tatgaccgct tgtacactcg tttaggaaag caaaatgaat
     4861 atttcaggca aattacaggg ttggcactat gccctactgt gctacttaac ttggggaacc
     4921 tttccgattt attggtatcc gatcaaccaa tcgggaatgc ctgccgagca aattttggct
     4981 caacgggtgc tttggtctgc cctatttgct gtgtttctgc tgctgatttt ccaacaaggg
     5041 cgagcggtta ttaatgcctt taaacagcca aaggtactcg ggctgttcgt gctttcttct
     5101 tttatgattg gcgtgaactg gctggtttac ttatgggcaa tcgtcaatga gcatatttta
     5161 gatgccagtc taggttattt tatcaacccc attttcaacg tgtttgtcgg taggcttatt
     5221 ctaaaagagc agctgaataa agcccaatta ttggctctgc tgtttgcaat tagcggcatt
     5281 ttatggcttg ctattcctgc cggacaaatt ccgtgggtcg cattgattct agccgccagt
     5341 ttctgtattt acgggctgat tcgtaaactt gccccaatgg aacctttgtc tggtttggcg
     5401 ttagaaactt tgctgatgtc gccttttgcc atcacctatc tctgcttttg ttaccaccaa
     5461 aacattttgg tattcagtga actcaatcct ttacaaatgg cagtgctact ctgctccggc
     5521 attgccacta ccttaccatt gctatggttt gcaatgggtg caagacgcat ttcaatgtcg
     5581 ctactcggta tgctgcaata tatttcacct accctgcaat ttttatgcgg tgtgctgatt
     5641 tttaatgaga cactttccgc cgaacgtgct gtaggctaca ttctggtttg gattggggtg
     5701 ggggttttct tattgggtat gaaaaaagct ggtgtcaaac gctaaaaata aaacgttaaa
     5761 tatttcgttt tggtagccct aatgcagcaa aaccaagtag tggcaaatag gcacaggctt
     5821 taaaaatact ctccaagcca tattcatccg caaaaaaacc caatacggcg gcacttaagc
     5881 cgccaatacc aaacattaaa ccgaacattg tgccggaaat cagccctact ctgcctggta
     5941 cggcttcttg agcataaacc accatagcgg agaacgctga tgacataata aaaccggcaa
     6001 aaatcgcaca cacaatcgtc caaaagagat ctgcgtgagg caacaacaaa gcaaaaggtg
     6061 ccatacctaa gaaagagaac caaatcaccg catttcgtcc aatcttatcg ccaatcggcc
     6121 cgccgacaaa agcgcctaaa gcaactgcac ctaaaaaggt aaatagagag agctgtgcca
     6181 ttgctatatt gagttggaat ttttccatta aataaaaggt gaaatagtta ctgaggctcg
     6241 caatataaat aaatttcgct aacatcaata aagcgataat taataatgct ctggctaata
     6301 atttaccgtg taagcgttgt tgaatatggt ttggtgaaat aatgagcggt ttggcttcat
     6361 atttcaccca acggcttact ttatataaaa cccatcataa tgccaataat acaaatgcaa
     6421 caacccaaat caccgcactt tgccctaacg gcacaataaa taatgcaact aagagcggac
     6481 ctaacgccga tccactgttt ccacctactt ggaaaagcga ctgagctgta ccaaatttac
     6541 cgcccgatgc cattcgagcc acccgagagg cttcagggtg aaaaatggca gagcctatcc
     6601 ccattaacgc agcggctatc agtaacacgc caaaattcgg cgaaaaggca agtaaaatca
     6661 cggaaagaag cgtaatcagc atactgacgg gcaataaatt cggttttggg tatcggtata
     6721 aagcccaata cccggctgaa taatagatgc ggtaatttga taaatgagtg aaatcatacc
     6781 aatttgagca aaactcagtt ggtaattttc ttttagcatt ggatagatcg aaaccattac
     6841 tgcttgaact aaatcattca acaaatgaga aagggaaatc agcagtaaaa tgggcaaaac
     6901 cattttttga gcgttatgtt gtgtgtgcat tctttattcg ttttttataa atgaaaaata
     6961 ttctactcgc aaataatgaa tgtgcaagcg gtcaaatttc ccgatttttt taccaaaccc
     7021 attaggttgc aatattatta ccaatacgtc ttaaatttgt tatttttata tctttttgct
     7081 atattcaagc ctctaaccat tcaaaaatag aggaaatagc attggattta atcaaaaaat
     7141 taaacacagg aagtaccttt agggtaccga ttttcctacc gagtttactc tttgtcagct
     7201 ttgttgccgt tttctgtatc atctttccac agcaagcaca aacctcactt gataccatca
     7261 aaaatagtct cttccaacat tttagctggt tctatatttt tgcaggctct atctttttcc
     7321 tgtttctaat ttttctctct ttcagccgat tgggtgatat taaattaggg gcagataccg
     7381 atgagcctga atttggtttt ggctcttgga ttgcgatgtt attcgccgcc ggaatgggga
     7441 tcgggttaat gtattttggg gtagcagaac ctattttgca ttaccttaaa cccgtccaac
     7501 aaaatttaac tgagccggag cgtatgaaag aagcgatgat gacaacgttc tatcattggg
     7561 gtattcacgc ttgggcaatt tatggtgtga ttgccttagc tcttgcttat tttggcttca
     7621 gatataagtt agcactcact attcgttccg gattttatcc cttactaaaa catcgtattt
     7681 caggcttctg ggggcattta attgatatta ttgccctttg tagcacgatt ttcggtttaa
     7741 cgactacact tggctttggg gtgatgcagg tcagtgctgg ctttaacaat ctaggtttaa
     7801 ttgaacagag caattttact gttcttgcga ttatcgtaac agtagcaatg gctcttgccg
     7861 tgttatctgc cgtttcgggc gtaggcaaag gggttaaaat cttaagtgaa atcaatctca
     7921 cattagccgg attgctactt atttttgtga taatcaccgg cccaactcta ttacttttct
     7981 caagcttcac cgaaaattta ggctattatt ttagctcgct gcttgagatg agtttccgta
     8041 ccttcgctta tgaaccggaa catcaaggct ggctaagcgg ctggacggtc ctttattggg
     8101 catggtgggc atcttgggcg ccatttgttg gtttgtttat tgccaagatc tctaaaggca
     8161 gaaccattcg tgaatttatt ttaggggtgc tatttgttcc atcgctgttt aacattttat
     8221 ggatgaccag cttcggcagc tctgccattt ggttcgatca acaaactgcc ggtgctttag
     8281 ctgaagtcag cggcaatacc gaacaactgt tatttacctt ttttgagcaa ttaccgtttg
     8341 gctctattgc ctctttcgtt gccgtcattg ttatcagtat tttctttatc acctctgccg
     8401 actcggggat ttttgttctc aacagcattg cttcacaagg cgaagaaaat gcaccgaaat
     8461 ggcaaagcgt gctttgggga gcattattag ccatcttagc gttatcacta ctctattcgg
     8521 gtggcttggc ttctctgcaa acaatgacac tgattatcgc cttaccattt accttcatta
     8581 tgctgattct ctgtatcggc ttatggaaag gattaatggt agataaccaa tacttcaaca
     8641 aaaaattctc gcaaggtagc caacattggg cgggtaaaga ttggaaacaa cgcttggaga
     8701 aaatcatcaa cccaagcaat aagcaagatg tccgtcactt ctttattaaa gttgccagac
     8761 cagcattttt agaacttatc gaggaatttg aaagctatgg cttaatcgct aaaatgaatt
     8821 tcaccaacga acaaaacccg aaattagagt ttgaagtggt gaaagaaaat ttacgcaatt
     8881 tcatttacgg cattgaaagt gtgccacggg aattatcgga tttggtggta ggtgacgaca
     8941 acctaccgaa cattgagcaa aataccattt acgagccgat tacttatttc ttagacgggc
     9001 ggaaaggtta tgatgtgcaa tatatgacca aagaagagtt gattgccgac gtgctgcaac
     9061 agtatgaacg ctttatcaat ttagcgatgg acaactcgca cgacttaatg acggctgatt
     9121 tcaatcacta accccacaag cggtgagatt ttcggaaaaa tttgcaaatt tccaacaaaa
     9181 tctcaccgct tgcatttaaa gccaacgcct tacttggttg caatatttgg cataatgtgt
     9241 gccgaatttt tgggctaaaa tttgctcttc cggcttgatt tggaaatagg ttatcaacca
     9301 tacaaacagc ggtaaaacca gccacgccaa cattgccccc aaccataatg cccacgcaat
     9361 caaacctaat agtaacgaaa gatacattgg gtttcggctt ttgcggtaaa tgccccaagt
     9421 gaccaattta gcggtatgtt gtggtcgaaa aggactgtat gtcgttttcg cttggtgtat
     9481 gacccaaagt gccgagacgg caaccgctcc gccaattatc gccacagcta aaccgctaaa
     9541 aaatatggct gtatgctgat aattcggcaa gctcaccggc acatattgag ctaccagcca
     9601 cattaaagcg gcacaaagca agaaccaagc cggcggcggg attttcagtt ttaatgattt
     9661 cattttatgc cgagcggaaa tcttttttac gaatgcctaa taccaaaata gaggcaaaat
     9721 aactaccgcc ggctaagatc atcagccaaa tcagccaata cactttctgc cacattgtca
     9781 ttaatgccca aagttcaatc tcaggcgtga aataggcaac caacgccccc attacacaag
     9841 cggcgatgaa tagtttcaac acaaaaagca cggttttatg gcttacttta tagtaaccat
     9901 tttttgagag attcacatac agcaagctca cattcactaa tgccgaacac gcagaagcta
     9961 acgctaaacc aatgtatccc aaaaacggag caaacgccaa gccaaagcaa atattactta
    10021 aggcggcaat aatcccaact tttaccggtg ttttggtgtt ttggttggca taaaaaccgt
    10081 ttgccagcac gctaatcaac atatagctgt ttaaccccaa gcacattacc caaagtggat
    10141 aagaggttgc cagcacatct tcaaaaccaa atttaccccg cataaacatt gtcataataa
    10201 tcggctgagc cagcactgcc ataccgatca ttgccggaat gccgagcaac agcaccattc
    10261 tcacccccca atccattgtg ttttgaaagt gttctccacg ttggatttca gtgagttctt
    10321 tgtttttcgc aatacgggaa aggcttggca gcacgacggt ggaaatcgca attccaaata
    10381 aaccgagcgg aaattcaatt aagcggtcgg cataatacat ccaactaatc gagccggtaa
    10441 tcaaaaacga ggcaatcact tggtttagca gtaaatttag ctgggtaacg gaaacgccaa
    10501 ataaagccgg aatcattaat ttacggactt tagtgacacc ttcgtctttc cacgcccatt
    10561 ttggcttaac cagtaagccc tcttttttca taaatggaat ttggaataaa aattgcaata
    10621 agccgcctaa aaataccccc cacgctaagg caatatccgg ggaatcgaaa taatctctgc
    10681 cccataacgc taccgcgata atcgccacat tcagcaacac cggggaaaat gccatcaccc
    10741 caaacttacc gatggtattt aacaccgcac cggataacgc cacaaaggta ataaaccaca
    10801 aataagggaa agtgattttg agcaaaaaag aggcttgggt gaatttttcg gcatttggcc
    10861 cgtcgtaaag ccaatctaaa aaccaacctg taccaaaaag agcggcaacc acaggcgagg
    10921 caatcattgc cacaagggta acaacagtta ccaaacctcc taatgtaccc gatactttgg
    10981 caataaattc tctcgttttg tttggatcgt tatccgcatt atattccgcc aacaccggca
    11041 caaaggcttt agaaaatgcc ccttcggcaa acaaacggcg aagaaaattg ggaatacggt
    11101 tggcaaacag gaaaatatcg gcactcacgc ctgtgcctaa aatggttgcc accacaatgt
    11161 ctcgaatcaa accgagtatg cgagaaatta aggtcataaa gcttacgatc attcctgatc
    11221 ttaaaagttt tttgctcata ataaagagta atattagtaa aattattga
//
LOCUS       DANLRK010000081        11138 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.081, whole genome shotgun sequence.
ACCESSION   DANLRK010000081 DANLRK010000000
VERSION     DANLRK010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 11138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11138
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.081"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            41..1837
                     /locus_tag="PXA33_001520"
     CDS             41..1837
                     /locus_tag="PXA33_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate-specific subunit IIBC"
                     /protein_id="HDL5120842.1"
                     /translation="MDFLTEILLFLKDQVLNKAPFLLGIVACLGYILLKKDTSTIIKG
                     TIKTIVGFMIVQAGSGFLVTNFKPVIDGLAKYHQLTGAVIDPYTSMQSTIQTMGENYA
                     WVGYAVIGALFLNILLVVFRRLTGIRTIMLTGHIMFQQAGLVAVFYMIIGASMWETII
                     YTSVLMALYWGISSNIMYKPTQAVTGGAGFSIGHQQQIASWIAVKLAPKLGDKDDTVD
                     KMKLPNWLNIFHDSISATALVMTIFFGIILLSFGLDNLKELAGKTNQFMYILEMGLKF
                     AVAIQIIVTGVRMFVAELSEAFKGIAERLIPNSVLAIDCAAIYAFSPNAMVFGFMWGA
                     IGQFVAVGGLLAVGSPVLIIPGFIPMFFSNATIGVFANQFGGWKAVMKICFVMGIIEV
                     LGSAWVIQMLASQGTTFNGWMGMADWALFFPPILQGIISIPGFFFVIVAAAMVYMFFA
                     SKQLRAEEAEAAARGITLEQLDGYGLDEVKAEPKAEAVVQQAVENAENSANPIRILAV
                     CGSGQGSSMMMKMKIKGYLDKRGIPNIMDSCAVTDYKGKLSEVDIIVSSKHLANEIEV
                     PEGKAVLGVQNMLNPNSFGDELVELINQHNAK"
     gene            1917..2978
                     /locus_tag="PXA33_001521"
     CDS             1917..2978
                     /locus_tag="PXA33_001521"
                     /inference="COORDINATES: protein motif:HMM:NF012376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDL5120843.1"
                     /translation="MKKILSTLFMLSAVSVAVAKEVNIKLLGTSDVHGRVVPWSYGAD
                     VEDKSGSFAQIATYVKEVRANHKNVLLFDIGDAIQDNQVEEFAKTLDKAKNNPVPKVL
                     NAMNYDIFILGNHEFNFGMPNLDAILKDINAKILTANFYHKDGKRYVEPTTIIERDGV
                     KLGIIGLTTPMSAIFEKDTGHLDAMKFTSPIEEAKAQIADLKAKGVDAIIVLAHMGIE
                     NENNIPDTGVADLVNAVDGIDVIIAGHMHKDVPSETIKNTLITEPHRYGTVVSEVDLA
                     FDVNDKGEVKLLSKTAKTVPVKKLASDPEIEKIYQPYHNELRALNNVKIGETAQDLIP
                     QGKITVYRLLSRKTPECPH"
     gene            2966..3430
                     /locus_tag="PXA33_001522"
     CDS             2966..3430
                     /locus_tag="PXA33_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HDL5120844.1"
                     /translation="MSSLIHDVQQHYSGADVVSFAFDYETVRLDKGDIKKKDIIYNYR
                     YAGGDVSMYELTGKQLKQYMEWSADYFDTIQAGDKDYRYNAVRGKSKYVTFDLFGGVS
                     YNIDLRNPSGSKIVDLKLANGSLITDDGKYKVGMNSYRFGQLTKKGGIWEGQ"
     gene            3461..3577
                     /locus_tag="PXA33_001523"
     CDS             3461..3577
                     /locus_tag="PXA33_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ser/Thr protein phosphatase"
                     /protein_id="HDL5120845.1"
                     /translation="MGAEKGTIQNMLIDYITNVKGGKIEGKSHNRWAIIGLN"
     gene            3593..4060
                     /locus_tag="PXA33_001524"
     CDS             3593..4060
                     /locus_tag="PXA33_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL5120846.1"
                     /translation="MNLKQSLIENNSILLNQSAANWEDAIKIGTDLLVKAGTIEPRYY
                     DNIVSKIKEMGPYIILAPGLAMPHARPEEGVIKTSFALVTFDTPIYFEGEDEPVHVML
                     TLAGSDSDQHMQGLVEITQVLDDPESDTGVDLDKIRRCKTAEEVYAVIDQALA"
     gene            4115..4795
                     /locus_tag="PXA33_001525"
     CDS             4115..4795
                     /locus_tag="PXA33_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HDL5120847.1"
                     /translation="MSKPLLQIALDSLSLEKAVADAKQAENQVEIIECGTILAFAEGM
                     KAVSTLRVLHPNHIIVCDLKTTDGGAILAKMAFEAGADWLTVSAAAHPATKAACKKVA
                     DEFNAAHPELKVKKEIQIEIYGNWTVEKDAKEWVELGVTQAIYHRSRDAELAGKGWTT
                     EDVELMKQLSDLGIELSITGGIVPEDIHLFKEIKNAKAFIAGRALVGEKGKQTAEAIR
                     VEIDKYWN"
     gene            complement(4863..6005)
                     /locus_tag="PXA33_001526"
     CDS             complement(4863..6005)
                     /locus_tag="PXA33_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDL5120848.1"
                     /translation="MKIVIAPDSFKESLTALEVAQAIQQGFARVFPEAEYQLVPMADG
                     GEGSVQSLVDATHGSLQKISVIAPLANEVEGFFGLSGDQKTAFIEMAAASGLHLVPME
                     QRNPLVTTSYGTGQLIKAALDLGVEKIILGIGGSATNDGGVGMLQALGAKFLNAEQRE
                     IGFGGEALSQIQQIDLSGLDSRLKQVEFEVACDVDNPLCGERGASAIFGPQKGATPEM
                     VKMLDHALLHFAETVKSQLGIEIAEKAGAGAAGGMGGGLLLLPNVTLRSGVQIILEAT
                     DLAEKLADADLVVTGEGRMDAQSIAGKTPIGVAKVAKQFGKPVIAIVGSLKEDYPVVY
                     QHGIDAVFPIIRQVSNLEAVLKSGKENLISTAENVARLWKISALQE"
     gene            complement(6015..7394)
                     /locus_tag="PXA33_001527"
     CDS             complement(6015..7394)
                     /locus_tag="PXA33_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005757929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HDL5120849.1"
                     /translation="MSGIALILIFLVAIIIMIYMIAKWKVHPFLALMSISIALALVAG
                     LPLAKIPTIVGEGFSNTFKSIGIVIIFGAIIGTILEKTGAALKLADMVVKVVGQKRPE
                     LAMLIMGWVVGIPVFCDSGFVVLNPIREAIRKKILANPVGMAVALSAGLYASHVFIPP
                     TPGPIAAAGAVGLSHNLLLVIGMGFVVSIPVLIASYFFAKYVGKQVSVMEEAQADQVI
                     SQSYEDLIKQYGKLPSGFLSFAPIFMPIILMAIGSIAKIAGVSGDFGVFLQFVGNPII
                     ALAIGVIFAIFLLVSSGKMAEFDSLTNDTLKLVGPILFITAAGGVLGNVITQAGFVDY
                     IKQNAHIISTAGIFFPFLISAILKTAQGSSTVAIITTASIMGMFNAPDSLMNALGLTS
                     EMAAALTVMAIAAGSMCVSHANDSYYWVVTNFTKLTPQQGYRTQTTLTFIMGVVGMLT
                     VYLLSLILL"
     gene            complement(7516..8610)
                     /locus_tag="PXA33_001528"
     CDS             complement(7516..8610)
                     /locus_tag="PXA33_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL5120850.1"
                     /translation="MKLNQAIADRIVKRTMQIIPNSVNVMDSEGVIIASGDVGRIGQR
                     HTGAVIALRNDQAVEIDENLAKQWCDEVKAGINLPLHYLGSTIGVVGISGQPQQVRQY
                     AQLVKMAAELIMQQAFELEQERWQRRYREEFVRALLKGSLSTAEIQQQAVFFGQDFAK
                     PLSVMVIKIHGSTAEKLQRLIDFFEHYFPSLLTAVIGLDKIALLNHLSNEKSLIRNAL
                     NKLNDPDISFKTVVGLAVENLSQTHISYQTACHTLNYAEQIQSRKQIIHFEDFKLPAL
                     LHSFSHSWEAKTLLSPFQALLVSDKKQVLLKSLRYYFFANCDLDHAAKKLFIHPNTLR
                     YRLEKIEQITGLSFNNIEQKFVLYLGAVLS"
     gene            complement(8714..9166)
                     /locus_tag="PXA33_001529"
     CDS             complement(8714..9166)
                     /locus_tag="PXA33_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HDL5120851.1"
                     /translation="MNLKAVAVSALAMSSVAMADIADVKPVVFESKFDFQTTVTTLTN
                     TLKEKGMTIFAEIDHQAAAKQAGLEMQPATVLVYGTPKAGTPLMVKDPMLALQLPLKV
                     LITEPNKGKVEVMLNRADQVVAHANTDYADVENNLAKAEKLIKATVAK"
     gene            complement(9187..9939)
                     /locus_tag="PXA33_001530"
     CDS             complement(9187..9939)
                     /locus_tag="PXA33_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HDL5120852.1"
                     /translation="MHFEKRSHWSGELGREMSFNVYGHAGKPVIVFPSSGGNENEYGN
                     FGMIEACRSFIERGLIKFYTPDSYDAESWLSKHKSGHDMALAHNAYDRYIIHELVPLI
                     RHESHWNGTMIATGCSMGAFHTVNFALRHPDLFDIAIALSGVYDARFFSGEFYGDKAV
                     YFNSPIDYVWGQNDDWFLDRYRQNHYVIAVGQGAWEEQHIEDTRRLQEAFNAKGIDAW
                     FDYWGFDIDHDWPTWRKQMPYFLGKLEEQGII"
     gene            complement(9950..11134)
                     /locus_tag="PXA33_001531"
     CDS             complement(9950..11134)
                     /locus_tag="PXA33_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HDL5120853.1"
                     /translation="MSRPLNFVMISPHFPTNFETFAIRLREAGFNTLGIADAPYESLS
                     ENLRHSLTEYYRVDNMEDYEQVYRAVAYFAHKYGRIDRIESHNEYWLELDAKLRTDFN
                     VFGYKSDDMLSIKTKAQMKEVFRKTGLKVAQGRVFKDDEDARKLAKQLKFPVIIKPNS
                     GVGASDTYKIKTAEELEAFFGYKTPTVEYIMEEFIDGDIITFDGLTDKEGNIVFYSSL
                     EYSEAVLDTVSKDGDMFYYVPCEISPKLVEMGKQCVKAFNVKERFFHFEFFRVKGSKK
                     EELLPLEINCRPPGGLTIDMWNYANDFDVFREFANIVKENKFYADIQHPWNVVYISRK
                     ANQNYVNSIEAVCEKFAGNIISVQSVPGVFAKVMGEQGILARTETMEQMREIAQFAQA
                     KQ"
BASE COUNT         3199 a         2570 c         2344 g         3025 t
ORIGIN      
        1 gttttttagt tctattaatc tctcaacaaa gaggccccct atggactttt taaccgaaat
       61 tctccttttc ttaaaggatc aagtgctgaa taaagcaccc ttcttactag gtatcgtagc
      121 ttgcttaggt tacattcttc ttaaaaaaga cacttctacg attattaaag gcacaattaa
      181 aacaattgtc ggttttatga ttgtacaagc cggttcaggt ttcttagtaa ccaactttaa
      241 gccggttatt gatggcttag ccaaatatca ccaacttacc ggtgcagtaa ttgaccctta
      301 caccagtatg caatcgacca ttcaaaccat gggcgagaac tacgcttggg ttggttatgc
      361 cgtaattggg gcgctgttcc tcaatattct attagtggtg ttccgccgtt taaccggtat
      421 ccgtaccatt atgctcaccg gtcacattat gttccaacaa gcaggcttgg ttgcggtgtt
      481 ctatatgatc atcggagcgt caatgtggga gaccattatc tacacctcag tgttgatggc
      541 tctttattgg ggtatttcgt ctaatattat gtataaacca acccaagcgg taacaggcgg
      601 tgcaggcttc tcaattggtc accaacaaca aattgcttct tggattgcag tcaaactcgc
      661 accaaaactt ggcgataaag acgatactgt agataaaatg aaattgccta actggctcaa
      721 tattttccac gacagtattt ctgcaaccgc attagtaatg accatcttct ttggaatcat
      781 cttactttca ttcggtttag ataacctcaa agaacttgct ggcaaaacca accagtttat
      841 gtacattctt gaaatgggct tgaaatttgc ggtggcaatt caaatcattg taaccggcgt
      901 acgtatgttt gtagctgagc tttcagaagc cttcaaaggt attgctgaac gcttaattcc
      961 aaactcagta ttagcgattg actgtgccgc tatctacgct ttctcgccaa atgcgatggt
     1021 attcggcttt atgtgggggg ctatcggtca atttgtggca gtaggcggct tgcttgcagt
     1081 aggctcaccg gtgcttatta ttccgggctt tatcccgatg ttcttctcta acgcaaccat
     1141 tggtgtgttt gcaaaccaat ttggtggttg gaaagcagta atgaaaatct gttttgtgat
     1201 gggtattatt gaagtgcttg gctcagcttg ggttattcaa atgcttgctt cacaaggcac
     1261 aacctttaac ggttggatgg gtatggctga ctgggcattg ttcttcccac caatcctaca
     1321 aggtattatc agcattccgg gcttcttctt tgtaattgtg gcagcagcaa tggtttatat
     1381 gttcttcgca tctaaacaac ttcgtgccga agaagcagaa gcggcagctc gtggcattac
     1441 acttgaacaa ttagacggct acggcttaga tgaagtaaaa gcagaaccaa aagcggaggc
     1501 agtagtacaa caagcggtcg aaaatgccga aaattctgca aacccaatcc gcattctagc
     1561 ggtgtgtggc tctggtcaag gctcatcaat gatgatgaaa atgaagatca aaggctatct
     1621 cgacaaacgt ggtattccga atattatgga ttcctgtgcg gtaactgatt acaaaggcaa
     1681 attaagcgag gttgatatta ttgtctccag caaacattta gccaacgaaa tcgaagtacc
     1741 ggaaggcaaa gcggtattag gtgtacaaaa tatgctaaat ccaaactcat ttggtgatga
     1801 attagttgag ctaatcaatc agcataatgc aaaataagta agtttagggg tgaataatca
     1861 cccctatcta tgctaaagat tgtaaaaact cacttcatca aacgaggaat tatccaatga
     1921 aaaaaatctt atccaccctg tttatgctct ccgcggttag cgttgccgtt gccaaagagg
     1981 taaacattaa actacttggt acctcagacg ttcacggacg tgttgtgccg tggagctacg
     2041 gggcagacgt ggaagacaaa tccggctcat tcgcccaaat tgcaacctat gtcaaagagg
     2101 tgcgtgccaa ccacaaaaat gtgttgctgt ttgatattgg cgatgcgatt caagacaacc
     2161 aagtcgaaga gttcgctaaa accttagaca aagcgaaaaa caatcccgtg ccgaaagtgc
     2221 tgaatgcaat gaattacgac atcttcattc tcggcaacca cgaatttaac ttcggaatgc
     2281 cgaatttaga tgcgatttta aaagacatca acgccaaaat tctcactgcc aacttctatc
     2341 acaaagacgg caaacgctac gttgagccaa ctaccatcat tgaacgtgat ggcgtaaaac
     2401 ttgggattat cggcttaacc accccaatgt cggctatttt tgaaaaagac accggtcatt
     2461 tagacgcaat gaagttcact tcgccgattg aagaggcgaa agcccaaatt gccgatctca
     2521 aagccaaagg cgtggacgcc attattgtgc tggcacatat ggggattgag aacgaaaaca
     2581 acattcccga caccggcgtg gcggatttag tgaatgcagt ggacggtatt gatgtaatta
     2641 tcgccggcca tatgcataaa gatgtgccga gtgaaactat caaaaatacc ctgattaccg
     2701 aaccgcaccg ctacggcaca gtggtttccg aagttgatct tgcctttgat gtgaatgaca
     2761 aaggcgaagt gaaactactt tctaaaaccg ccaaaaccgt accggtgaag aaactcgcaa
     2821 gcgatccgga aatcgaaaaa atttaccaac cgtatcacaa tgaactgcgt gccttaaaca
     2881 acgtcaaaat cggtgaaact gcacaagatt taatcccaca aggcaaaatc acggtgtatc
     2941 gattgctttc tcgcaagaca ccggaatgtc ctcattgatc cacgatgtgc aacaacatta
     3001 cagcggtgca gacgtagtga gttttgcatt tgactacgaa accgtgcgtt tagacaaagg
     3061 cgacatcaag aaaaaagaca tcatctacaa ctaccgctat gccggtggcg atgtgagcat
     3121 gtatgagcta accggcaaac agcttaaaca gtatatggaa tggtcagcgg attacttcga
     3181 caccattcaa gccggtgaca aagactaccg ctacaacgct gtgcggggta aatctaaata
     3241 cgttaccttc gacctattcg gcggcgtaag ctacaacatc gacctacgca atccaagcgg
     3301 tagcaaaatt gtcgatctaa aactggcaaa cggcagtcta atcaccgatg acggcaaata
     3361 caaagtggga atgaactcct accgctttgg tcagctcacc aaaaaaggcg gcatctggga
     3421 aggacagtaa atccccgtga tttgggaatc caaagtcgcc atgggggctg aaaaaggcac
     3481 aatccaaaat atgctgattg attacatcac caacgtcaaa ggcggaaaaa tcgaaggcaa
     3541 atcgcataac cgctgggcga ttatcggctt aaattaacga aagggaaaca ctatgaactt
     3601 aaaacaatct cttattgaaa ataactctat tttgctcaat caatcagcgg cgaattggga
     3661 agacgcaatc aaaatcggca ctgatttatt agtcaaagcc ggtacgattg aaccacgcta
     3721 ttacgataat attgtaagca aaatcaaaga gatggggcct tatattattc ttgcaccggg
     3781 cttggcaatg cctcacgccc gtccggaaga aggtgtgatt aaaacctctt tcgctttagt
     3841 gacttttgac accccgattt attttgaggg cgaagatgag ccggttcacg ttatgctaac
     3901 ccttgcaggg agtgactctg atcagcatat gcaaggcttg gtagaaatca cccaagtgct
     3961 agatgaccca gagagcgaca caggtgttga tttagataaa atccgccgct gtaaaacggc
     4021 tgaagaagtg tatgcagtaa tcgatcaggc attagcctaa tattctacaa gcggttcaaa
     4081 aacatcaata ttttgcaaat agaggaatac aattatgtcc aaaccattat tacaaattgc
     4141 ccttgattct ctttcactgg aaaaagcggt ggcggatgca aaacaagcag aaaaccaagt
     4201 cgaaattatt gaatgcggta ctattcttgc ttttgctgag ggtatgaaag ccgtttccac
     4261 cttgcgtgta ttgcacccaa atcacattat tgtgtgcgac ttaaaaacca ccgatggcgg
     4321 tgcaatttta gctaaaatgg catttgaagc cggtgccgac tggctcacgg tttcggctgc
     4381 ggctcaccct gcaaccaaag cggcttgcaa aaaagtggcg gacgaattta acgctgctca
     4441 cccggagcta aaagtgaaaa aagagattca aattgaaatc tacggcaact ggacggtgga
     4501 aaaagatgcc aaagagtggg tagaattagg ggtaacccaa gcgatttacc accgctcccg
     4561 tgatgcggaa ctagcaggta aaggttggac aacggaagat gttgagttaa tgaaacagct
     4621 ttctgactta ggcattgaat tatctatcac cggcggtatc gtaccggaag atattcacct
     4681 gttcaaagag atcaaaaacg ccaaagcctt tattgcaggc cgtgctttag tcggtgaaaa
     4741 aggcaaacaa actgccgaag ccattcgtgt ggagattgat aaatactgga actaatccgg
     4801 cattgaaacc caataaaaat ggcgttctct caaccggaac gccatttttt atctcatttt
     4861 ttctattctt gcaaggccga tattttccac aatctcgcta cattttcggc agttgaaatc
     4921 aggttctctt tgcctgattt taacaccgct tctaaattgc tgacctgtcg aataatcgga
     4981 aataccgcat caatgccgtg ttggtacacc acagggtaat cttctttcaa tgagccaact
     5041 atcgcaatca ccggcttgcc aaactgctta gcgactttcg ccaccccaat cggggtttta
     5101 ccggcgatag attgagcgtc cattcgccct tcccctgtca ccaccaaatc cgcatcggca
     5161 agtttctcag caagatcggt tgcctcaaga ataatttgca cgccggaacg taatgttacg
     5221 ttaggcagta gcagtaaacc accgcccatt ccaccggctg caccggcacc ggctttctcg
     5281 gcaatctcaa tgcctaattg ggattttacg gtttcggcaa aatgcaacag ggcgtggtcg
     5341 agcattttaa ccatttccgg tgtagctcct ttctgtggcc caaaaattgc cgaagcccct
     5401 cgctcgccac acagcggatt atcgacatcg caagcaacct caaattccac ttgctttaag
     5461 cgtgaatcca aaccgcttaa atcaatctgc tgaatttggc ttaatgcctc gccaccaaag
     5521 ccgatttccc gctgttcggc atttagaaat ttcgctccta aggcttgtag cattccgaca
     5581 ccgccatcgt tagtcgcact gccgccaatg ccgagaatga ttttttccac ccctaaatct
     5641 aaggcggctt taattaactg ccccgtgccg tagctggtgg taaccagcgg atttcgttgc
     5701 tccattggca ctaaatgcaa gccacttgcc gccgccattt caataaaggc ggttttttga
     5761 tcccccgaca agccaaaaaa gccctccacc tcatttgcaa gcggtgcaat tacgctgatt
     5821 ttttgcaaac tgccgtgagt ggcatcaacc agcgactgta ccgagccttc gccaccgtct
     5881 gccatcggta cgagctgata ctccgcctca gggaaaacac gggcaaaacc ctgttgaatt
     5941 gcttgagcca cttctaatgc ggtcaaactc tctttaaatg aatccggtgc aatcacaatt
     6001 ttcatcgtca atccttagag taaaatcaat gaaagcagat aaaccgtgag catacccacc
     6061 acacccataa tgaaagttag ggtagtttgc gtacgatagc cttgttgcgg agtcagttta
     6121 gtaaagtttg ttaccaccca gtagtagctg tcattggcgt gggaaacgca cattgagcca
     6181 gccgcaatcg ccattacggt taaggctgcc gccatttcac tggttagccc taaggcgttc
     6241 attaatgagt ccggtgcgtt gaacattccc ataatggagg cggtggtgat aattgccacc
     6301 gttgagctgc cttgtgcggt ttttaaaatt gccgaaatca ggaacgggaa gaaaatccct
     6361 gctgtactga taatatgagc gttttgcttg atgtaatcca caaagccggc ttgagtaatc
     6421 acgttaccta gcacaccacc ggcggcggta ataaagagaa ttgggccgac taatttcagg
     6481 gtatcattgg ttaagctgtc aaattccgcc attttaccgc ttgaaaccag taggaaaata
     6541 gcgaagatca ccccaattgc taacgcaata atcgggttgc cgacaaattg taggaatacg
     6601 ccgaaatcgc cacttacacc ggcaattttg gcaatcgagc cgatagccat taagataatc
     6661 ggcataaaaa tcggggcaaa gcttaaaaac ccacttggta atttgccgta ttgtttgatc
     6721 agatcttcat agctttggct gattacttga tccgcttgag cttcttccat cacgctgact
     6781 tgtttgccaa catatttggc aaagaagtaa ctggcaatca acacaggaat cgaaaccaca
     6841 aagcccattc cgatcacaag caggagatta tggcttaaac caacagcacc ggctgctgca
     6901 atcgggcccg gtgtcggagg aataaagacg tgggaggcat ataaaccggc acttaacgcc
     6961 accgccattc ccaccggatt ggcaagaatt tttttgcgaa tagcttcacg aatcgggttt
     7021 aacaccacaa agccgctatc acagaacact gggataccaa ccacccagcc cataatcaac
     7081 attgccagtt cagggcgttt ttgccccacc acttttacca ccatatcggc taatttgagg
     7141 gctgcaccgg ttttttctaa aatcgtgccg ataatcgccc caaaaataat cacgataccg
     7201 atacttttga aggtattact aaaaccttcg cccacaatag ttggaatttt ggcaagcggt
     7261 aaacctgcca ctaatgcaag ggctatcgaa atcgacatta aggctaagaa cgggtgaact
     7321 ttccacttcg caatcatata aatcattatg atgatagcga ccagaaagat cagaataagt
     7381 gcaatacctg acataataag tctcctcatt gtagaaaaat gaacttatta ttcggtattt
     7441 attgctaaaa agatttagat aaatttgcaa aaaatttgtt aaaaacgacc gcttgtttgt
     7501 atatttttac aatttttacg acagaaccgc tccaagatac agcacaaatt tttgctctat
     7561 gttattgaaa gataaaccag ttatctgctc aatcttctct aaacggtaac gtagcgtatt
     7621 tgggtgaata aacaattttt tggcagcgtg atccagatca caatttgcga aaaaatagta
     7681 acgcagactt ttcagtaaca cctgtttttt atcggaaacc agtaaagctt ggaacggcga
     7741 gagcaaggtt ttcgcttccc aagagtggct gaaactgtgc aagagggccg gcagtttaaa
     7801 atcttcaaaa tgaataatct gcttacggct ttggatctgc tcggcataat tgagggtatg
     7861 gcaggcggtt tggtaggaaa tatgggtttg cgataaattt tcgaccgcca gcccaaccac
     7921 cgttttgaag gaaatatccg gatcgttaag tttgtttagt gcattgcgga ttaacgattt
     7981 ttcgttggat aaatgattca gcaaggcaat tttatctaac ccaatcacgg cggtgagtag
     8041 gctcgggaaa taatgttcga aaaaatcaat caggcgttgg agtttttccg cagtagagcc
     8101 gtgaatttta atcaccatca ccgacagcgg ttttgcaaaa tcttgcccga aaaagaccgc
     8161 ttgttgctgg atttctgctg tgcttaggct acctttgagc aaggcccgca caaactcttc
     8221 ccgatagcgg cgttgccaac gttcctgttc caactcaaac gcttgctgca taatcagctc
     8281 cgccgccatt ttcaccaatt gagcatattg ccgaacttgt tgtggctgcc ctgaaatccc
     8341 cactacacca atggttgagc ctaaatagtg aagcggtaag ttaatgcccg ctttcacttc
     8401 atcgcaccac tgttttgcaa gattttcatc aatttcgacc gcttgatcgt tgcgtaacgc
     8461 aatcaccgct cctgtgtgtc gttgcccgat acgaccgaca tcgccggaag caataatcac
     8521 cccttcacta tccatcacat tgacggagtt gggaataatc tgcatcgtgc gcttgacgat
     8581 gcggtcggca atcgcttggt tgagtttcat tatatgattc ctactcattc ccaatactat
     8641 gaaaaatccc tctttcgagg gatttgcaaa ttatctacgc ttccacttcc tgagaataga
     8701 tcatagcgtg aagttatttt gccacagtcg ctttaattaa tttttccgct ttggcaaggt
     8761 tgttttctac atcagcataa tcggtattag cgtgagccac cacttgatcg gcacgattta
     8821 acattacttc gaccttgccc ttgttcggtt cggtaattaa cacttttaac ggtaattgca
     8881 acgctaacat cggatctttc accattaatg gcgtaccggc tttcggtgtg ccgtaaacca
     8941 atacggttgc cggttgcatt tctaagccgg cttgttttgc cgccgcttgg tgatcgattt
     9001 cggcaaaaat cgtcattcct ttttctttta aggtgttggt taaagttgtg actgtagttt
     9061 ggaaatcaaa tttactttca aataccaccg gtttgacatc ggcaatatct gccattgcca
     9121 cgctgctcat tgctaatgca gaaactgcga ctgcttttaa gttcatttct tttctcctca
     9181 aatagattaa ataatccctt gttcttccaa cttgcctaag aaataaggca tctgtttacg
     9241 ccaagtcggc cagtcatggt caatatcaaa gccccagtag tcaaaccaag catcgatccc
     9301 ttttgcgtta aaagcctctt gtaatcggcg ggtgtcttca atatgctgtt cttcccacgc
     9361 accttgtccg actgcaatca catagtgatt ttggcggtag cggtctaaga accaatcgtc
     9421 attttgaccc catacataat caatcggcga gttaaaataa accgctttat cgccataaaa
     9481 ctcaccactg aaaaagcgag cgtcataaac accgcttaag gcgatggcaa tatcaaacag
     9541 atccggatga cgtaaggcaa agtttaccgt atggaaagcc cccatactac aaccggtggc
     9601 aatcatcgtg ccgttccagt gcgattcatg gcggattaac ggcactagct cgtgaataat
     9661 ataacggtcg taagcattat gagctaatgc catatcgtgt ccggatttat gtttagataa
     9721 ccaagattcc gcatcgtaag aatcaggcgt gtaaaatttg attaagccac gttcaataaa
     9781 ggaacggcaa gcctcaatca ttccaaaatt accatactcg ttttcattgc cgccggagga
     9841 agggaaaaca ataaccggtt tacctgcgtg tccgtaaacg ttgaacgaca tttcacgacc
     9901 taattctcca ctccagtggc ttcttttttc aaaatgcata cttcacctct tactgtttag
     9961 cttgtgcaaa ttgtgcaatt tcacgcattt gctccattgt ttcggtacgg gctaaaatgc
    10021 cttgttcgcc catcactttg gcaaatacgc ccggtacgct ttgcacgctg ataatattgc
    10081 cggcaaattt ctcacaaaca gcttcaatcg aattcacata attttggttt gccttgcggg
    10141 aaatataaac cacgttccaa gggtgctgaa tatcggcata gaatttgttt tctttcacaa
    10201 tattggcaaa ttcacggaac acatcgaaat cgttggcgta gttccacata tcaatagtta
    10261 agccacccgg cggacggcag ttgatttcga gtggtaacaa ctcttctttt ttgctccctt
    10321 taacacggaa gaactcaaaa tggaaaaaac gctcttttac gttaaaggct ttcacacatt
    10381 gtttgcccat ttccactaat tttggcgaaa tttcacatgg gacatagtag aacatatcgc
    10441 catcttttga aacggtatct aataccgctt ctgaatattc aaggctggag tagaaaacga
    10501 tattgccctc tttatcggtt aaaccgtcaa aggtaataat atcgccgtca ataaactctt
    10561 ccataatata ttcaaccgtt ggcgttttat aaccgaagaa agcttctaac tcttccgcag
    10621 ttttgatttt gtaagtatcg cttgccccaa cccctgaatt tggcttgata atcaccggga
    10681 atttaagctg ttttgctaat ttgcgggcat cttcatcatc tttaaatact cgcccttgtg
    10741 ccactttcaa gccggttttg cggaatacct ctttcatttg ggctttggtt ttaattgaga
    10801 gcatatcatc gctcttataa ccaaacacat tgaaatcggt acgcaatttc gcatctaact
    10861 ccagccaata ttcattgtgc gattcaatgc ggtcaattcg cccgtattta tgggcaaagt
    10921 acgcgaccgc acgataaact tgttcgtaat cttccatatt atctacacga taatattcag
    10981 tcagggaatg acgcagattt tcgctcaatg attcataagg ggcatctgca attcccaatg
    11041 tattaaaacc ggcttcacgc aaacgaatgg caaaggtttc aaagttcgtt gggaagtgcg
    11101 gggaaatcat cacaaaatta agtggtctag acatagtt
//
LOCUS       DANLRK010000082        11110 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.082, whole genome shotgun sequence.
ACCESSION   DANLRK010000082 DANLRK010000000
VERSION     DANLRK010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 11110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11110
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.082"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(2..4462)
                     /gene="mukB"
                     /locus_tag="PXA33_001532"
     CDS             complement(2..4462)
                     /gene="mukB"
                     /locus_tag="PXA33_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HDL5120854.1"
                     /translation="MIDTTELQNNPQIAPLARPNDVQRGKFLSLTLINWNGFFARTFD
                     LDDLVTTLSGGNGAGKSTTMAGFVTALIPDLTLLNFRNTTEAGSTAGSRDKGLYGKLK
                     AGVCYAVLESFNSRGQRIITGVRLQQIVGRDKKVDIRSFSLQNIPQEQSIISLLTEQV
                     GNKARVLALNELKDQFENSEVQFKQYHSITDYHSFMFDLGVIPKRLRSAADRSKFYKL
                     IEASLYGGISSVITKSLRDYLLPENTGVRQAFQDMESALRENRMTLEAIKVTQSDRDM
                     FKRLITESTNYVSADYMRNANERRGNVQQALEQRKEWYAAKSKILLEQQRFVEFSRES
                     ADIAEAELALEADYNSANDHLNLVMNALRHQEKIERYQDEVEELNIKLEEQQEALEEI
                     AEIAENAQARADEADDHVEELRSQMADYQQALDAQQTRALQYQQAVNALEKAKQLTGL
                     VNLDLNNIEDYHAEFVAQAEDLTDQVFELEQRLSVSDMAKTQFEKAFELVCKISGEID
                     RLQAWEEARALLSAFPEQKMQAQQAVSLRQKLNDLEQRLQQQQNAQRLVAEFNQKSQQ
                     QLNSAEELDAYFEEQQARLEDLEAELSDFVEQRSTQRQQREQLNQQYQQLAKNAPAWH
                     TAQSALTRLEEQCGEKFEASQSVMQFMQNMLSKEREATLARDELARKEQMLDEQINRL
                     SQPDGSEDARLNQLAERFGGVLLSELYDDVSIEDAPYFSALYGEARHAIVVRDLEAVK
                     TQLEKLDDCPSDLYLIEGDPSAFDDAVFSAEELGEGVVVKVSDRQWRYSKFPEVPLFG
                     RAAREKHLATLKAERDETAEKHAERAFDVQKCQRLHQHLSQFIGTHLNLAFQENPEML
                     MQEIAAERAEIERELAQSAGNEQQLRHQLESVKAQLQMLNKILPLANLLADETLEARA
                     EECREQLELAEEDEMFIRQFGATLSQLEPIAAALKSDPALFEQLEADYRRSVAEQKVL
                     QQKVFSLADVMNRRLHFSYQETVGTEGSALTEQLRQRLENAQREREQARDQLRQAQAQ
                     LSQYNQVLTGLRSSYEAKNQMLQELIREIDDLGVRGDSGAEERARFRRDELQQRLSQQ
                     RSRKGYLDKQIAVIEAEMDNLNRTLRKAERDYHTQRELVVQAKASWCIVQRLSRNSDV
                     EKRLNRRELAYQSAEELRSISDKALGALRTAVADNEYLRDSLRASEDSRKPENKVAFF
                     IAVYQHLRERIRQDIIKTDDPIDAIEQMEIELSRLTNELTGREKKLAISAESVANILR
                     KTIQREQQRILQLNQGLQNIAFGQVKGVRLVVNIRDTHAILLNALSNNREEHKDLFDS
                     QKLSFSEALAMLYKRVNPHIEMGQRTPQTIGEELLDYRNYLDLEVETFRGADGWMRAE
                     SSALSTGEAIGTGMSILLMVVQSWEEEARRMRAKDILPARLLFLDEAARLDATSINTL
                     FELCERLDMQLLIAAPENISPERGTTYKLVRKITNNQEYVHVVGLKGFAK"
     gene            complement(4638..5354)
                     /gene="mukE"
                     /locus_tag="PXA33_001533"
     CDS             complement(4638..5354)
                     /gene="mukE"
                     /locus_tag="PXA33_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HDL5120855.1"
                     /translation="MTENIQDFISPKLALAIANPIFPELDSQLRAGRHINTEQLDQHV
                     FLMDFQAELESFYRRYHVELIRAPEGFFYLRPKASTLIARSAMSEMEMLVGKVLCYLY
                     LSPERLAQQGIFSQDDVYEELLSLADESKLLKAVNPRSTGSDLDRAKLAEKAGGALRR
                     LARIGIITRVGEQNSKKFVISESVFRFGADVRTGDDPREAQLRLIRDGEATTPALLNE
                     QAVEIDENIADEAPFDGENE"
     gene            complement(5403..6746)
                     /gene="mukF"
                     /locus_tag="PXA33_001534"
     CDS             complement(5403..6746)
                     /gene="mukF"
                     /locus_tag="PXA33_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HDL5120856.1"
                     /translation="MQNELAQTIPELISWTKDREFSLSLSSDRLAFLLAISIYNQEQT
                     DGELLESDLIDLFRYVSNSFEQSDATFTQRANNAINDLVRQRLLNRFSSEFTEGLAIY
                     RVTPLGVGISNYYVRQREFSTLRLSIQLSIVADEIQRASVAAEEGIEQAQDERFWRNN
                     VFAPLKYSVAEIFDSIDLSQRIMDENQHQIRERIAELLSQNWHEAIMSCEQLLDETSG
                     NLRELQDTLNAAGDKLQAQLLRIQSCLMARNDLDFIDQLIVNLQNKLDRIISWGQQAI
                     DLWIGYDRHVHKFIRTAIDMDKNRVFGQRLRQSIQDYYNSPWLLYTAKADSLLDLRDD
                     EAMLNEAESVGELPSELEYESLSDVQEQIVSAMQAHLAPFRAEGKPIDLGVILREQLA
                     RFPESRHFDVARIIVDQAVKLGMASQDSQAVYPQWQPINEQGAEVQANVIDQYNK"
     gene            complement(7011..7898)
                     /gene="galU"
                     /locus_tag="PXA33_001535"
     CDS             complement(7011..7898)
                     /gene="galU"
                     /locus_tag="PXA33_001535"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDL5120857.1"
                     /translation="MKVIIPVAGLGTRMLPATKAIPKEMLILADKPLIQYIVNECVAA
                     GFKEIVLVTHSSKNAIENHFDTSFELETMLEKRVKRQLLDDVRSIVPKDVTLIHVRQG
                     QAKGLGHAVLCGRTVVGDEPFAVVLPDVLLGEFTANQKTENLAAMVKRFQETGYSQIM
                     VAPVPMENVSSYGVADCHGVDIPLGGAAPIAKMVEKPSIEEAPSNLAVVGRYVFSSNI
                     WNLLEKTPVGVGDEIQLTDAIDMLIHQETVEAFHMTGRSFDCGYKVGYMKAFVEYSLN
                     HDKCGKEFKAFIKELAKSL"
     gene            complement(8034..8219)
                     /gene="csrA"
                     /locus_tag="PXA33_001536"
     CDS             complement(8034..8219)
                     /gene="csrA"
                     /locus_tag="PXA33_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HDL5120858.1"
                     /translation="MLILTRKIGEVLLVGDDVEITVLSIRGNQVKLGVNAPKEISVHR
                     REIYERIKASVEEENAE"
     gene            complement(8309..10936)
                     /gene="alaS"
                     /locus_tag="PXA33_001537"
     CDS             complement(8309..10936)
                     /gene="alaS"
                     /locus_tag="PXA33_001537"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005622703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HDL5120859.1"
                     /translation="MKTTSDIRQSFLEFFQTKGHTIVPSSSLVPENDPTLLFTNAGMN
                     QFKDVFLGLEKRPYSRATTAQRCVRAGGKHNDLENVGYTARHHTFFEMMGNFSFGDYF
                     KQDAIKFGWEFLTSPQWLGLPKEKLYVTVYETDDEAYDIWHKEVGVPSEHIIRIGDNK
                     GAPYASDNFWAMGDTGPCGPCTEIFYDHGPEHWGGLPGSPEEDGDRYIEVWNIVFMQF
                     NRLADGTMEKLPKPSVDTGMGLERMTAVMQHVNSNYETDIFQTLIKEVATLLNVKDLD
                     NKSLRVVADHIRACSYLIADGVLPSNEGRGYVLRRIIRRAVRHGNILGAKEAFFYKLV
                     PTLATVMGQAGEVLTQKQAHIQKTLKAEEEQFARTLERGLNLLEEALAKVENNTLSGE
                     VAFKLYDTYGFPLDLTADVCRERDIKIDEDGFNAEMTAQRERAKASSSFGTDYNNVIK
                     VDSKTCFSGYTETSLNDATVIGLFSNGKSVESIQSGENAVVILDKTPFYAEMGGQVGD
                     SGQISAEICNFEVVDTQKYGQVFGHIGQLTSGSLSVGDKVNTIVDRERRHAITLNHSA
                     THLLHSALRQVLGDHVAQKGSLVSENILRFDISQPEAITKSQLEEVERIVNAKVRENI
                     QVIIEEMDIESAKAKGAMALFGEKYGEVVRVVEMSDFSIELCGGTHVKQTGDIGLFKI
                     TSEGAVAAGVRRIEAVTGENAIAWLHTLQQAVQQSAELLKADSHSFVEKIVQLQEKAK
                     RTEKELQQLKDKLAAQAGSDLAKQATQINGVNVVIQQLENVEPKALRTMVDDLKNQLG
                     SAVVVFATTAEDKVNLIVGVTKDLTDKVNAGQLVGAMAEKVGGKGGGRPDMAMAGGSE
                     PQHLLQALSLAQEWITAKL"
BASE COUNT         2991 a         2592 c         2121 g         3406 t
ORIGIN      
        1 tttatttagc aaagcctttt aaaccaacaa cgtgaacata ctcttggtta ttggtgattt
       61 tacgcaccaa tttataagtt gtgcctcgtt cagggctaat attttcaggg gctgcaatta
      121 ataactgcat atccaaacgt tcgcagagtt caaatagcgt attaattgag gtagcatcta
      181 atcgagccgc ttcatctaag aacaataagc gagccggtag aatatctttg gcacgcatac
      241 ggcgagcttc ttcttcccag ctttgaacta ccattaagag aatcgacata cctgtaccga
      301 tcgcctcacc ggtcgataat gcactacttt ctgcacgcat ccagccgtct gcaccacgga
      361 aggtttccac ttctaaatct aagtagttac ggtaatccaa taactcttca ccaatggtct
      421 gtggtgtacg ttgccccatc tcaatatgag ggttcacccg tttatacaac atcgctaagg
      481 cttcggagaa gctcagtttt tggctgtcga ataaatcttt gtgttcctca cgattattgg
      541 ataacgcatt gagtaaaata gcgtgcgtat ctcggatatt caccaccaaa cgcacccctt
      601 ttacctgacc gaatgcaata ttttgtaaac cttggttcag ttgcaaaatg cgttgttgtt
      661 cacgctgaat ggttttacgt aaaatatttg ccacactttc cgcactgatc gccagttttt
      721 tctcacgacc ggttaattca ttggttaagc gtgaaagctc gatttccatc tgctcaatcg
      781 catcaatcgg atcatcggtt ttgataatgt cttggcgaat ccgctcacgc aaatgctgat
      841 aaaccgcaat aaagaacgcc actttgtttt ccggcttgcg actatcttca gaagcccgca
      901 agctatcacg caggtattca ttatccgcca cggcggtgcg taatgccccg agagctttat
      961 ccgaaatcga acgtaattct tcagccgatt gataagccaa ttcacggcga tttaaacgtt
     1021 tttccacatc gctgttacga gataaacgtt gcacaataca ccagctggct tttgcctgca
     1081 ccaccaattc acgttgtgta tgataatcac gttcggcttt acgtaaggtg cggttaaggt
     1141 tatccatttc cgcctcgatc accgcaattt gtttatctaa ataacctttg cgggaacgtt
     1201 gctggcttaa acgttgttgc aattcatcac ggcggaatct ggctcgctct tccgctccgc
     1261 tatcaccacg cacgcccaaa tcgtcaattt cacgaattaa ctcttgtagc atttggtttt
     1321 tcgcctcata agaactgcgt aagccggtca atacttggtt atattgtgaa agttgagctt
     1381 gagcttgacg cagctgatca cgagcctgtt cacgctcacg ttgtgcattt tccaaacgtt
     1441 ggcgtagctg ttcggttaat gcagagcctt ctgtacccac cgtttcttga tagctaaaat
     1501 ggaggcggcg attcattaca tcagccaagc tgaacacttt ttgctgtaac actttttgct
     1561 cggcaactga acggcggtaa tcggcttcca actgttcaaa taaagccgga tcacttttta
     1621 atgcggctgc aatcggctca agttggctta atgttgctcc aaattggcgg ataaacattt
     1681 catcttcttc tgccaattct aattgctcac gacattcctc cgctcgtgct tccagcgttt
     1741 catcggctaa taaattcgca agcggtagaa ttttattcaa catttgcaac tgggctttta
     1801 cgctttctaa ttggtgacgt aattgctgtt cgttaccggc tgattgtgcc aattcacgtt
     1861 caatttctgc ccgttctgcc gcaatctctt gcattaacat ttcagggttt tcttggaacg
     1921 ctaaatttaa gtgcgtgccg ataaattggc ttaagtgttg atgtaaacgc tggcatttct
     1981 gcacatcaaa ggcacgttca gcgtgttttt ctgcggtttc atcacgctct gcttttaaag
     2041 tcgctaaatg tttttcacga gcagcacgtc cgaataacgg cacttcaggg aatttagaat
     2101 aacgccattg gcgatcggag actttcacca ccacgccttc gcctaattcc tcggcactaa
     2161 ataccgcatc gtcaaaggcg gacggatcac cttcaattaa ataaagatca cttgggcaat
     2221 cgtctaattt ctctagctgt gttttaaccg cctctaaatc acgcactaca atagcgtggc
     2281 gagcctcgcc gtataaggcg gagaaataag gcgcatcttc aattgataca tcatcatata
     2341 actcagagag taacactcca ccaaaacgtt ctgccaactg gtttaagcgg gcgtcttccg
     2401 agccgtccgg ctggcttaag cgattaattt gctcatcaag catttgttct ttacgagcca
     2461 attcatcacg agcaagggtg gcttcacgct ctttgctcag catattttgc ataaactgca
     2521 tcactgattg gctggcttca aacttctcgc cacattgctc ttctaaacga gtcagagccg
     2581 attgtgccgt atgccaagcc ggtgcattct tcgctaattg ctgatattgt tgattaagct
     2641 gttcacgttg ttggcgttgg gttgaacgtt gttctacaaa gtccgaaagc tctgcctcta
     2701 aatcttctag gcgagcttgc tgctcttcaa aataggcgtc taattcttcc gctgaattga
     2761 gttgttgttg cgatttttgg ttaaattccg ccactaaacg ctgggcattt tgctgttgtt
     2821 gcaaacgctg ttctaaatcg ttgagttttt gacgtaaact caccgcttgc tgagcttgca
     2881 ttttttgctc agggaaggca gaaagtaacg cacgagcctc ttcccacgct tgcaagcggt
     2941 caatttcacc ggaaattttg caaaccaatt caaacgcttt ttcaaattgg gttttcgcca
     3001 tatccgacac gcttaaacgt tgttccagct caaatacttg atcggtaaga tcttcggctt
     3061 gagccacaaa ttctgcgtgg taatcttcaa tattatttaa atctaaattc actaagccgg
     3121 ttagctgttt ggctttttct aacgcattca ccgcttgttg gtattgcaat gcacgagttt
     3181 gctgtgcatc taaggcttgt tggtaatccg ccatttgaga acgcaattct tcaacgtgat
     3241 catctgcctc atccgcacga gcctgagcat tttcggcaat ttccgcaatt tcttccaatg
     3301 cctcttgctg ttcttccagc ttaatattca gctcttcaac ttcgtcttgg tagcgttcga
     3361 ttttctcttg gtggcgtaac gcattcatca ctaaatttag atgatcgttc gcactattgt
     3421 aatccgcctc taacgccagc tctgcttcgg caatatccgc actttcacgg ctaaattcca
     3481 caaagcgttg ctgttcgagc aaaattttgg attttgccgc ataccactct ttgcgttgct
     3541 ctaaggcttg ttgtacattg ccacggcgtt cattcgcatt acgcatataa tccgccgaca
     3601 cataattggt cgattcggta attaagcgtt tgaacatatc ccgatccgat tgcgtgacct
     3661 taatcgcctc taaggtcata cggttttcac gcagagcgga ttccatatct tggaacgctt
     3721 ggcgaacgcc cgtattttcc ggcaacaagt aatcacgcaa ggatttggta atcacacttg
     3781 aaataccacc ataaagcgag gcttcgatta atttgtagaa tttgctacgg tcggctgccg
     3841 aacgaaggcg ttttgggatt acgcccaaat cgaacataaa actgtggtaa tcagtaatcg
     3901 aatggtactg tttgaattgg acttcgctgt tctcaaattg atctttgagt tcatttaacg
     3961 ccagcactcg agccttattc cccacttgtt cggtcagtag gctgataatg ctttgctctt
     4021 gaggaatatt ctggagcgag aacgaacgaa tatcgacttt tttatcccgt ccgacaattt
     4081 gttgcaaacg cacgccggta ataatccgtt gcccacggga attgaatgac tctaacaccg
     4141 catagcaaac acccgctttt aacttaccgt ataagccttt atcacgagaa ccggcggttg
     4201 agcctgcttc agtcgtatta cggaaattaa gcaaggttaa atctgggatc aaggcggtca
     4261 caaaccctgc cattgtggtg gacttacctg ccccgttacc gcctgaaagc gtggtcacta
     4321 agtcatctaa atcaaaggtt cgagcaaaaa aaccgttcca gttaatcagc gttaaagaga
     4381 gaaatttacc gcgttgtaca tcattcgggc gtgcaagcgg tgcaatttga ggattatttt
     4441 gcaattctgt ggtatcaatc attatgtttt ctcactaatt gtcctagcac gctaaagcgt
     4501 actaggttag gtataggtga gtagctccgc tactctaatc gcaacctcag ccttggaggg
     4561 ctgctttatg cttaacctag ttcggcttta ccgtgtctag ggcagactca ttaaatgctg
     4621 aatctattcg aatgaaatca ctcattttct ccgtcaaagg gggcttcatc tgcaatattt
     4681 tcatctattt caaccgcttg ttcgtttaac aacgcagggg ttgtcgcttc accatcacga
     4741 attaagcgaa gctgtgcctc tcttgggtca tcaccggtac gcacatcagc cccaaaacgg
     4801 aagaccgatt ctgaaatcac aaattttttg ctgttttgct cgcctactcg ggtaataatg
     4861 ccgatacggg ctaatctgcg taacgctccg ccggcttttt ctgccagctt ggcacggtct
     4921 aaatcggaac cggtcgaacg tgggtttacc gcttttaata atttgctttc atcggcaaga
     4981 ctgagtaatt cttcatacac atcatcttgg ctgaaaatcc cctgctgagc caaacgttcc
     5041 ggacttaaat agaggtagca caacacctta cccactaaca tttccatttc tgacattgcc
     5101 gaacgggcaa ttagtgtcga ggctttcggg cgtaggtaga aaaagccttc cggtgctcga
     5161 atcaactcaa cgtgataacg gcggtaaaaa ctctctagtt ccgcttgaaa atccatcaaa
     5221 aagacgtgtt ggtcaagctg ctcggtattg atatggcgac cggctcgtaa ttggctgtct
     5281 agctccggaa aaatcgggtt ggcaatcgca agtgccagtt tcggggaaat gaaatcttgg
     5341 atattctctg tcattgtcat tctcttcaat tttgcaaaat tctgggtaaa aacgaccgct
     5401 tgttatttat tgtattggtc gataacattc gcctgcactt ccgccccttg ctcgttaatc
     5461 ggctgccatt gtgggtaaac ggcttggcta tcttggcttg ccatacctaa cttaacagct
     5521 tgatcgacaa taatacgagc cacgtcaaaa tggcgggatt ccgggaatcg ggcaagctgt
     5581 tcacgcaaga taacgcctaa atctatcggt ttaccttcag cacggaacgg cgctaaatga
     5641 gcttgcattg ccgaaacaat ttgctcttgc acatctgaaa gcgactcata ctctaactca
     5701 cttggcaact cgcccaccga ttctgcctca tttaacattg cttcatcatc acgcaagtcc
     5761 agtaacgaat cggcttttgc agtataaagc aaccaagggc tgttgtaata atcttgaatc
     5821 gattggcgta accgttgccc gaatacacgg tttttgtcca tatcaatcgc tgtacgaata
     5881 aatttatgca catggcggtc atagccgatc caaagatcga tagcttgttg tccccagctg
     5941 ataatacggt ctaacttatt ttgtagattc acaatcagct gatcgataaa atccaaatca
     6001 ttacgagcca tcaggcaact ttgaatccgc aataattgag cttgcagttt atcaccagcg
     6061 gcatttaagg tatcttgcaa ctcacgcaaa ttccccgagg tttcgtctaa caattgctca
     6121 caactcataa ttgcttcgtg ccagttttgg ctaagcagtt cggctattcg ttcacggatt
     6181 tggtgctgat tttcgtccat aatccgttgt gaaagatcga tgctgtcaaa gatttccgcc
     6241 accgaatatt tcaatggggc aaacacatta tttcgccaaa aacgttcatc ttgtgcctgc
     6301 tctattccct cttccgctgc tactgaagct cgctgaattt catctgccac aatcgaaagt
     6361 tgaatcgata aacgcagggt agaaaattcc cgctggcgaa cgtaataatt tgaaattccc
     6421 acccctaacg gcgtaacacg ataaatcgcc aaaccttccg taaattcgct actaaaacgg
     6481 tttaacaacc gttgtctgac taaatcatta atcgcattat tggcacgctg ggtaaaagtg
     6541 gcatcagatt gctcaaacga atttgaaaca taacggaaaa gatcaataag atcactttct
     6601 aacaattcac catctgtctg ttcttggtta taaatagaga tagcaagtag aaacgctaag
     6661 cggtcggaag aaagcgaaag tgaaaattca cgatctttcg tccaagagat gagttctgga
     6721 atagtctgtg ccaattcgtt ctgcatacgg gcttaatctt taatttctat acaaaaatgg
     6781 tatttattgt aactgaatgt aatcaaaatg ggaatggata aaacgcagac aagagaaatg
     6841 aacaatataa gcggtcaaat atgggtgatt ttttgcaaaa agtattttaa aggtaaatga
     6901 atccgaaaat ttattatcac tacgctaatt tatgatacca taaatagaaa agactggatc
     6961 caatagaata tcagacacag ccccttagat catagtctaa actctaaggc ttagagtgat
     7021 ttagctaact ctttaataaa tgctttaaat tcttttccgc atttgtcatg atttaaacta
     7081 tattctacaa atgctttcat atagccgact ttataaccac aatcaaatga gcgccctgtc
     7141 atatgaaaag cttcaaccgt ttcttggtga atcaacatat caattgcatc cgtcagctgg
     7201 atttcatcac ctacgcctac tggcgttttt tctaataaat tccaaatatt tgaggaaaat
     7261 acataacgtc ctactaccgc taaattagaa ggcgcctctt caatacttgg tttttcaacc
     7321 attttcgcaa taggcgctgc ccccccgaga gggatatcta caccatggca gtcagcaacc
     7381 ccatagctac ttacattttc cattggaacc ggcgcaacca taatttggct atatccggtt
     7441 tcttggaaac gcttcaccat cgcagctaaa ttttccgttt tttggtttgc tgtaaattca
     7501 ccaagtaata catctggtaa tacaaccgca aacggttcat cgccaaccac tgttcgtcca
     7561 catagaaccg catgacctaa acctttggct tgcccttggc gaacgtgaat taaggtaaca
     7621 tcttttggta caatagaacg tacatcatct aataattgac gttttactcg tttctccagc
     7681 atcgtttcca attcaaaaga ggtatcaaaa tggttttcga tagcattctt tgaagagtga
     7741 gtaaccaata caatttcttt aaaacctgca gccacacatt catttacaat gtattgaata
     7801 agaggtttat ctgcaaggat aagcatttct tttggaatag ctttggttgc gggtaacatg
     7861 cgcgttccca atccagcaac aggaataatg actttcattt ggaatccttt gatgacttaa
     7921 catattagat ataactgaaa taaaccgctt agacataaca ggcggtcaat tttcagaaaa
     7981 ttttgcattg cctgcttcgc tgttatcatc tagcagcttt acagttaaaa tgcttattcc
     8041 gcattttcct cttctactga ggcttttatt ctttcataaa tctctcggcg gtgtacagag
     8101 atttcttttg gtgcattcac acctaattta acttgatttc ctcggatgct gagtaccgta
     8161 atctcaacat catctcctac gagtaaaact tccccaatct tacgagttag aataagcata
     8221 attgtctcct aaaaagtgaa aaactctata aatacttaaa tatagagtaa agttttaaag
     8281 gcgagttcag ctcgccttgt tattattttt aaagttttgc tgtaatccat tcttgagcaa
     8341 gtgacaatgc ttgcaataaa tgttgtggtt ctgagcctcc ggccattgcc atatcaggac
     8401 gtccgccccc tttacctccc actttttcag ccattgcacc aactaattga ccagcattga
     8461 ctttatcggt taaatctttg gtcacaccta caatcaaatt gactttatct tccgccgtag
     8521 tcgcaaatac aacaactgca ctgcctaatt gattttttag gtcatcaacc atcgttctca
     8581 atgcttttgg ttcaacattc tctaattgtt gaatcactac attcacgcca ttgatttggg
     8641 tagcttgttt ggctaaatct gaacctgctt gtgctgcaag tttatctttt agctgttgaa
     8701 gctctttttc tgtacgttta gccttctctt gtaattgaac aattttctca acgaatgaat
     8761 gactgtctgc ttttaacaat tcagcacttt gttgaacggc ttgttgtaag gtatgcaacc
     8821 aagcaatcgc attttcacca gtgacagcct ctatacggcg tacacctgct gctaccgcac
     8881 cttcagaggt aattttgaat aagccaatat caccggtttg tttaacgtgt gtgccgccac
     8941 aaagttcaat cgagaaatct gacatttcca ctacacgcac tacttcgcca tatttttcac
     9001 caaataacgc cattgcacct ttcgcttttg cagattcaat atccatttct tcaatgatta
     9061 cctgaatatt ttcacgcact ttagcattca cgatacgttc aacttcttca agttgtgatt
     9121 tagtaattgc ttccggctga gaaatatcaa aacgtaaaat attttctgac actaatgagc
     9181 ctttttgagc aacgtggtcg cctagcactt ggcgtaacgc agaatgcagt aagtgggtag
     9241 cactgtggtt taatgtaata gcatgacgac gctctctatc tacgatcgta ttaactttgt
     9301 cgccaacaga aagtgagccg gaagttaatt gcccaatatg cccgaatact tgaccatatt
     9361 tttgcgtatc tactacctca aaattgcaaa tttctgcgga aatttgaccg ctatctccca
     9421 cttgtccgcc catttctgca tagaatgggg ttttatctaa aatcactacc gcattttcac
     9481 ccgattgaat agactcaacc gattttccat tactaaataa cccaattaca gtagcatcat
     9541 ttaagctggt ttcagtatat ccactaaagc atgttttact gtccacttta atcacattgt
     9601 tataatctgt gccaaagcta ctgctggctt tcgcacgctc acgttgtgcg gtcatttctg
     9661 cattgaagcc gtcttcgtca attttaatat cacgttcacg gcaaacatct gcggttaaat
     9721 ctaacgggaa gccataagta tcataaagtt tgaacgcaac ttcgccagat aaggtgttat
     9781 tttcaacctt cgctaatgct tcttctaata aattcaatcc acgctcaaga gtacgggcaa
     9841 attgctcttc ttctgctttg agtgtctttt gaatatgcgc ttgtttttgg gtcagcacct
     9901 caccagcttg ccccattacc gttgcaagtg ttggcaccaa tttatagaaa aaggcttctt
     9961 ttgcacccaa aatattaccg tgacgaaccg cccggcggat aatacggcgt aatacatagc
    10021 ctcttccttc atttgaaggt aaaacaccat cggcaatcag ataggaacaa gcacgaatat
    10081 ggtctgccac tacacgtaaa gatttattat ctaaatcttt cacattaagc aaagtagcga
    10141 cttctttaat taaagtttgg aaaatatccg tttcgtagtt ggagtttacg tgttgcatta
    10201 ccgcagtcat acgttctaac cccatccctg tatctacaga aggcttaggc agtttttcca
    10261 tcgtaccgtc tgctaaacga ttgaactgca taaatacaat attccatacc tcaatatagc
    10321 ggtcgccatc ttcttctggg ctacccggta agccacccca atgttcaggc ccgtgatcat
    10381 agaaaatctc ggtacaaggg ccacaagggc cggtatcgcc catcgcccag aagttatctg
    10441 aagcataagg tgcgccttta ttatcgccaa tgcgaataat atgttccgat ggtacaccta
    10501 cttctttatg ccaaatatcg taggcttcat catcagtttc atacacagtt acatataatt
    10561 tttcttttgg taagccaagc cattgtggag aagttaaaaa ctcccaaccg aatttgattg
    10621 catcttgctt gaagtaatca ccaaagctga aattccccat catttcaaaa aaagtatgat
    10681 ggcgagcggt ataacctacg ttttctaaat cattgtgttt accacctgca cgtacacaac
    10741 gttgtgccgt agtcgctctg ctataagggc gtttttctaa cccaagaaaa acatctttga
    10801 attggttcat gcccgcattg gtaaataata atgtcggatc attctcggga acaagagagc
    10861 tgcttggaac aatcgtatgt cctttagttt ggaaaaactc tagaaaagat tgtctgatat
    10921 ctgaagttgt tttcattaaa aatcactctt tttgtaaata gaagttaaac atttagttat
    10981 cttgctattt tacacatttt ttatgctatt gggggagcta tatgctactt attttttcaa
    11041 ttaaataatt tttgtaaatt ttcccaaaaa aataaccgct tgtaagacag ataaactgtc
    11101 tcaagcggtt
//
LOCUS       DANLRK010000083        10988 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.083, whole genome shotgun sequence.
ACCESSION   DANLRK010000083 DANLRK010000000
VERSION     DANLRK010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 10988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10988
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.083"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(19..1308)
                     /locus_tag="PXA33_001538"
     CDS             complement(19..1308)
                     /locus_tag="PXA33_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDL5120860.1"
                     /translation="MDSIPLSSLFITLAILLVLSAFFSGSETGLMSLNRYKMRHLAEQ
                     GHKGAKLAEKLLARTDVLLSLILICNNLVNIAASAIATMIGMQLSGEAGVAIATGLLT
                     FVMLVFSEILPKTVAAIYPEKVGFFASFVLTPLKKLLSPIVFLMNLIIAGLMKLLRIQ
                     QKDKQGLSSEELRSVVLDAGKFIPAEHREMLVSILDMEKVTVDDIMVPRNDISGIDID
                     DDWKAIMRQLNHAPHARVIIYKGDMDNNIVGILRVREAFRLMLEKNEFTKEMLLRATD
                     EVYFIPEGTPLTTQLMNFKANKERIGLVVDEYGDIKGLVTLEDILEEIVGEFTTSSAP
                     TLEQEVQQQSDGTVIIEGSANLRDLNKLFDWDLPLEEARTFNGLILEHLEKIPDEGME
                     FELYNLKVMILEVSENMVKQAKVEHLSPKIEKEQDSA"
     gene            complement(1432..2229)
                     /gene="ccsA"
                     /locus_tag="PXA33_001539"
     CDS             complement(1432..2229)
                     /gene="ccsA"
                     /locus_tag="PXA33_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HDL5120861.1"
                     /translation="MLLPLLSIVAYALALLWVAPTLVNLENAATVNKKPNMMLVFGFG
                     LLATILHFSVLYNELFLIEGQNFTVTNVISLMSFIMAACVTLALLKWKTVWFPLIIVY
                     SFAICSVGVAGFVKGSFIRNVQENAGLLFHLGIALFSYALFFLALIYAFQLKWLDRKL
                     KSKTMFFCQILPPLMTVERHFFTLTLAAQAMLTLTLFSGVVYLHNFFEPEHIHKAIFS
                     FLAWLVYSIQLLGQWKLHWRGKRVLIYSISGMMLLTIGYFGSRLIVN"
     gene            2445..2969
                     /gene="yceD"
                     /locus_tag="PXA33_001540"
     CDS             2445..2969
                     /gene="yceD"
                     /locus_tag="PXA33_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005621677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HDL5120862.1"
                     /translation="MQKVKLPLTIDPYKDAQRRVDYEGYISGKLLTRLGESVCNVQSD
                     AQVTLSLYVDPQRLTVIKGTAKVDVEFDCQRCGDPFTQTLDCSFVFSPVSNMDQADIL
                     PEIYEPIEVNEFGEVNLLDMIEDEFILELPLVPMHSEEHCEVSVSEQVFGELPEELVQ
                     KPNPFAVLANLKKN"
     gene            2986..3156
                     /gene="rpmF"
                     /locus_tag="PXA33_001541"
     CDS             2986..3156
                     /gene="rpmF"
                     /locus_tag="PXA33_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006231206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HDL5120863.1"
                     /translation="MAVQQNKKSRSRRDMRRSHDALTTAAVSVDKTSGETHFRHHVTA
                     DGYYRGRKVINK"
     gene            3175..4197
                     /gene="plsX"
                     /locus_tag="PXA33_001542"
     CDS             3175..4197
                     /gene="plsX"
                     /locus_tag="PXA33_001542"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HDL5120864.1"
                     /translation="MVDITLAVDVMGGDFGPRVTIPALEKALKQNPSLSFLLFGDEHQ
                     IAPLLQKHTLLNHPKIQIHHTEQVIEADVPFTTAIRQSKNSSMRLAIEAVKEGKAQGC
                     VSGGNTGALMGLAKLLIEPLPNIERPALTSLIPTLNGKSSVMLDLGANIEVSNHQLQQ
                     FAEMGNLFAQVMLDLVYPRLSLLNIGTEENKGTAQLQAVHQLLKNRQDLNYTGFIESD
                     KLTSYLTDVIICDGYTGNIALKALEGAAKNIISLFKKEKADSNICRNTKRYLLKAMFY
                     RYYRKLQQINPDRHNGATLLGLSKVVVKSHGGANINAFYYAIDYAVQQIQGDIPNKIL
                     EGLTQK"
     gene            complement(4294..5577)
                     /gene="hisD"
                     /locus_tag="PXA33_001543"
     CDS             complement(4294..5577)
                     /gene="hisD"
                     /locus_tag="PXA33_001543"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HDL5120865.1"
                     /translation="MQTLIWNNLTETEQQQYLARPAQVVGESIKAAVNEIKANVIANG
                     DKALFELAEKFDKVKLESLVVSEQQIQQAQSRISPELKQAIQNAKANIEAFHKAQQNQ
                     EVDIETQPGVCCQVITRPINAVGLYIPGGSAPLFSTVLMLAVPAKIAGCNKIVLCSPP
                     PIADEILYTAHLCGVETIYAVGGAQAIIAMANGTQTVAKVDKIFGPGNAFVTEAKRQV
                     VQEGTAIDMPAGPSEVLVIADEFADPAFVASDLLSQAEHGADSQVILVTPSESLARAV
                     DEELEKQVAVLPRAETARKALAHSRTFIAENLQQAVEISNKYAPEHLIVQTENARSLL
                     ADLDNAGSIFLGAYSPESMGDYASGTNHVLPTYGYTKTYSSLGLADFSKRMTVQELTP
                     QGFLALAETVERMASAELLDAHRNAVSLRVAKLKG"
     gene            complement(5589..6488)
                     /gene="hisG"
                     /locus_tag="PXA33_001544"
     CDS             complement(5589..6488)
                     /gene="hisG"
                     /locus_tag="PXA33_001544"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HDL5120866.1"
                     /translation="MTTQNRLRIAMQKKGRLSQECSELLKQCGVKIIWNDQRLIAYSE
                     NMPIEILRVRDDDIPGLVFDGVVDLGIIGENVLEEVELGRLSAGENVAYKKLRQLDFG
                     GCRLSLAIDRDKNYNNIKDLEDAVIATSYPNLLKRYMKEQNVPFKSILLTGSVEVAPS
                     AGIANAICDLVSSGATLEANGLKEVEVIYRSTACLIQRQEPLSAEKQALVDKLLTRIQ
                     GVQQANESKYIMLHAPKDKLAEITALLPGVENPTILPLANDQTRVAMHVVSQENLFWE
                     TMEQLKEMGASSVLVLPIEKMLA"
     misc_feature    complement(6521..6648)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            6847..7704
                     /locus_tag="PXA33_001545"
     CDS             6847..7704
                     /locus_tag="PXA33_001545"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HDL5120867.1"
                     /translation="MQISLKKIYSGKVRDLYEIDEKRMLMVATDRLSAFDVILDDPIP
                     RKGEILTQISNFWFNKLAHIMPNHFTGDSVFDVLPQEEAEAIQHRAVVCKRLIPVKIE
                     SIVRGYLTGSGLKDYQQTGTICGLTLPEGLVEASKLPQPIFTPSSKAEVGDHDINISY
                     EECERQIGQELAAKVRDAALKLYTEAANYALTKGIIICDTKFEFGLDENGELTLMDEV
                     LTPDSSRFWSVETYREGTNPPSFDKQFVRDWLENSGWNKQPPAPKIPAEVIEKTVAKY
                     QEALDLLTK"
     gene            complement(7845..9326)
                     /gene="ilvC"
                     /locus_tag="PXA33_001546"
     CDS             complement(7845..9326)
                     /gene="ilvC"
                     /locus_tag="PXA33_001546"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011199229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HDL5120868.1"
                     /translation="MANYFNTLNLRQKLDQLGRCRFMDRDEFADGCNFLKGKKIVIVG
                     CGAQGLNQGLNMRDSGLDIAYALRAEAIAEKRASFKRASENGFVVGTYEQLIPEADLV
                     INLTPDKQHSKVVADVMPLMKQGAAFGYSHGFNIVEEGEQIRPDLTVVMTAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGLAIAKAWASATGGDRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSIVCYDKLVSEGKDPAYAGKLIQYGWETITEALKQGGITLMMDR
                     LSNSAKLRAFELSEQIKEELAFLFEKHMDDIISGEFSSTMMADWANGDANLLKWREET
                     GKTAFENAPQADGIKISEQEYFDNGVLMVAMVKAGVELAFDTMVSAGIYEESAYYESL
                     HELPLIANTIARKRLYEMNVVISDTAEYGNYLFSHVATPILAEKIIPTLEKGDLGEPT
                     PAVEIDNVTLRDVNDAIRNHPIELIGQELRGYMTDMKRISSQG"
     gene            9548..10783
                     /locus_tag="PXA33_001547"
     CDS             9548..10783
                     /locus_tag="PXA33_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HDL5120869.1"
                     /translation="MKINRIILQSCSECGQQVRVPLTSNGNAVCPVCHNILRKSNTWS
                     LHRSAFLALAILILLPFALTFPLMSIDLLGIKINATVWDGIWKMATEGYPYTAFMVLV
                     CAVIMPIAFVLLILTIQLQRIIRQRPRYTLIFLTKIKEWVMLDVYLVALAVAAFKIRD
                     YADLQFDINLIAFVLVTLLNTLLFIKTEPKQAWERFYPEYHALPFDHPSNPTLCPTCE
                     YCFDETILDLKGRQRCPRCESNLSISDNVKIQRVWACLIAGTIMMIPANIFPISTTEL
                     AGQVSADSLMSGVLLFMEMGSYTVAAIVFIASIAVPVSKITIIFYLLLAIHYKWKHPI
                     HWQMKLLHYVHFVGRWSMLDLFVLSLMMSLLERGQILSFSVGEAAFYFGAAVFLTMFA
                     SANLDSRMLWKIHTVQKTS"
BASE COUNT         3267 a         2458 c         2074 g         3189 t
ORIGIN      
        1 ctttcttttt tcttaaaatt aggctgaatc ttgctctttt tcaattttcg gtgaaagatg
       61 ttcaactttc gcttgtttaa ccatattttc actcacttca agaatcatca ctttcaagtt
      121 gtagagttca aattccattc cttcatcagg aattttttct aaatgctcta aaatcaagcc
      181 gttaaaggtt ctggcttcct caagcggtaa atcccaatca aatagtttgt tcagatcacg
      241 caggttggct gagccttcaa taatcaccgt accatctgat tgttgctgaa cttcttgctc
      301 aagggtcggt gctgatgaag tcgtaaattc acccacaatt tcttctaaaa tatcttcaag
      361 agtgactagc cctttaatat caccatactc atcaaccact aaaccaatac gctctttatt
      421 cgctttaaag ttcattaatt gtgtagtgag tggtgtgcct tcaggaataa aatagacttc
      481 atctgttgca cgcagtagca tttctttggt aaattcgttt ttctccaaca ttaaacgaaa
      541 ggcttcacgc acccgcaaaa tcccaacaat attgttgtcc atatcccctt tgtagataat
      601 cacccgagcg tgaggggcgt gattaagctg acgcataatc gctttccagt catcatcaat
      661 atcaatgccg ctgatgtcgt tgcgaggcac cataatgtca tcaaccgtca ctttttccat
      721 atctaaaata gagaccaaca tttcacggtg ttcggcagga ataaatttac cggcatcaag
      781 cactacacta cgcagctctt cggagcttaa gccttgttta tctttttgtt gaatccgcaa
      841 taatttcatc agcccggcaa taatcaaatt cattaaaaat acaatcgggc tgagtaattt
      901 tttaagcggt gttagcacaa agctggcgaa aaaaccgact ttttcagggt aaattgccgc
      961 tactgtttta ggtaggattt ccgaaaaaac caacattaca aaagttagca aaccggttgc
     1021 aattgccaca ccggcttcac cggataactg cataccgatc atcgttgcaa tcgcagaggc
     1081 ggcaatgttg actaagttat tacaaattaa gataaggcta agtagcacat cggtgcgtgc
     1141 caagagtttt tccgccagtt tcgccccttt atgcccttgc tcggcaaggt gacgcatttt
     1201 gtagcggttt aaagacatca agccggtttc cgagcctgaa aaaaatgcag agagtactaa
     1261 aagaatagct aaggtaataa ataaactact caggggaata ctgtccaaaa ttcaattcct
     1321 atgtaaatta atcataaaag gtagcgtaac actaaaagcg ttgtcactac ctcactttgt
     1381 cggttcagcc gacttgcaaa aaattttgct tttttgaccg cttgtagatc attaattgac
     1441 gatcaaacga ctaccgaaat agccgatggt gagcaacatc atacccgaaa tcgaataaat
     1501 aagcacccgt tttccacgcc aatgtaattt ccactgtcca agtaactgaa tgctataaac
     1561 aagccaagct aaaaaggaga aaattgcttt atgaatatgc tccggctcga aaaagttatg
     1621 caaataaacc acaccggaaa ataaagtgag cgtcagcata gcttgtgcag ctaaggttaa
     1681 agtaaagaaa tggcgttcaa ccgtcattag cggtggtaga atctggcaaa agaacattgt
     1741 tttgctcttt agttttctat ccagccattt gagttgaaaa gcataaatta atgctaaaaa
     1801 gaagagagca taagaaaata atgcaatccc taaatggaaa agcagtcccg cattttcttg
     1861 cacattacgg ataaaactac cttttacaaa tccagccacc cccacactac aaatggcgaa
     1921 agagtacaca ataattaaag gaaaccaaac cgttttccat tttaacaacg caagtgtgac
     1981 acaagccgcc ataatgaaac tcatcaaaga gattacattt gttaccgtaa aattctgccc
     2041 ttcgattaaa aataattcat tatataacac agaaaaatgc aaaatcgtgg caagtaaccc
     2101 aaacccaaag actaacatca tattaggctt tttattaact gttgcggcat tttctaaatt
     2161 cactagagta ggggcaaccc aaagtagcgc tagagcgtaa gcaacaatgg ataaaagagg
     2221 aagtaacata ttgtctcgaa tctcgtcaaa taagaataac tatcagaatt ttaatcaaaa
     2281 gtaaccgttt tagctagttt ttgctgttgc tagatcaaaa attatcctaa aaaatgtgct
     2341 tttcgcctta aattgaaccc taccttttct cggtaagatt atctcactga aaacaataat
     2401 tttcctttga ctaatttgct ttctatctgt aatatgcaca ccctatgcaa aaggtaaaac
     2461 taccccttac gattgaccct tacaaagatg ctcagcgtcg cgttgattat gaaggctaca
     2521 tttcaggtaa acttctaaca cgcttgggtg aatcagtatg taatgtgcaa agtgatgcac
     2581 aagtcactct ctcgttatat gtcgatccac aacgcctaac tgtgattaaa ggcacagcaa
     2641 aagtagatgt ggaatttgat tgccaacgat gtggtgaccc gtttacacaa acgctcgact
     2701 gttcatttgt tttcagtccg gtgtctaaca tggatcaggc ggacatcttg cccgaaattt
     2761 atgaacctat cgaagtaaac gagttcggtg aagtaaattt actagatatg attgaagatg
     2821 aatttattct tgaattacct ctagtcccga tgcatagtga agaacactgt gaagtgtccg
     2881 taagtgaaca ggtatttggc gagttgccgg aagaactggt acaaaaacca aacccgttcg
     2941 cagtattagc taatttaaag aaaaactaga tctaggagaa agcccgtggc tgttcaacaa
     3001 aataagaaat ctcgttcacg tcgtgatatg cgtcgttcac acgatgcatt aacaactgca
     3061 gcagtttcag tagataaaac cagtggtgaa actcactttc gccaccacgt aactgcagac
     3121 ggttactacc gtggtcgtaa agtaatcaac aagtaattcc tttcgaggtt tatcttggtt
     3181 gatatcactc ttgcagtaga tgtgatgggc ggggactttg gtccccgcgt tactattcct
     3241 gcactggaaa aagccttaaa acaaaatcca agcctttctt ttttattatt tggtgatgaa
     3301 caccaaatag cccctttatt acaaaaacac acactactca accaccccaa aatccaaatt
     3361 catcataccg aacaagtgat tgaagctgat gttccgttca ctactgcaat ccgccaaagc
     3421 aaaaacagct ctatgcggtt agcgattgag gcagtaaaag agggcaaagc acaaggttgt
     3481 gttagtggcg gtaacactgg tgcgttaatg ggattggcaa aattattgat tgaaccatta
     3541 ccaaatattg aacgccctgc actcacttcg ctaattccta ccctcaacgg caaatccagc
     3601 gtaatgttag atttaggggc aaatattgaa gtaagcaatc accagctaca acaatttgca
     3661 gaaatgggaa acctgtttgc tcaagtaatg cttgatttag tttaccctcg cctgtcttta
     3721 ttgaatattg gtacagaaga aaataaaggc acggcacaac ttcaggcagt gcatcaactg
     3781 ctgaaaaaca gacaagactt aaattacacc ggctttattg aaagcgataa attaaccagc
     3841 tatctcactg atgtgattat ttgtgacggc tacaccggca atatcgcgtt aaaagcgttg
     3901 gaaggggcag ctaaaaatat tatttctctc tttaaaaaag agaaagccga ttccaatatt
     3961 tgccgtaaca ccaaacgata tttactgaaa gcgatgttct accgttacta ccgcaaattg
     4021 caacaaatta accctgaccg ccataacggt gcgaccttgc tcggcttatc taaagtagtc
     4081 gtgaaaagcc acggcggggc aaatatcaat gccttttatt acgctattga ttatgcggtg
     4141 caacaaattc aaggcgacat tccaaataaa atcctagagg gtttaaccca aaaataagcg
     4201 aactcaactg ttcgcttatt ttcatttctt gtttcactta aagatacaag cggtcaaatt
     4261 tgcaaaaaaa tttacccatt caaccgcttg tgtttagcct tttaattttg ccactcgtaa
     4321 actaaccgca ttgcggtgtg catctagcaa ttctgcactt gccattcgtt ccaccgtttc
     4381 ggccaaggct agaaagcctt gtggtgtcag ctcctgcacc gtcattcgct tgctaaaatc
     4441 agctaaacct aagctcgaat aggttttagt gtagccgtaa gtcggtaaaa cgtggttagt
     4501 gccgctagca taatccccca tactttccgg cgaatacgcc cctaagaaaa tcgagccggc
     4561 attatctaaa tccgccaaca acgagcgagc attttcagtt tgcacaatta aatgctcggg
     4621 tgcatattta ttactaattt cgaccgcttg ttgcaggttt tccgcaataa aagtgcgact
     4681 gtgagccaat gctttacggg cagtttcggc acgaggtaaa accgccacct gcttttctaa
     4741 ttcttcatcg actgcccttg ccaagctctc acttggtgtg actaaaatca cttgggaatc
     4801 ggctccatgt tctgcttgag agagtaaatc gctcgccaca aatgcagggt cagcaaactc
     4861 atcggcaatc accaacactt ccgacgggcc tgccggcata tcaatcgccg tgccttcttg
     4921 cacaacttgg cgtttcgcct ccgtcacaaa cgcattgccc gggccgaaaa ttttatccac
     4981 tttcgctacc gtttgtgtgc catttgccat cgcaataatc gcttgagccc ccccaactgc
     5041 gtaaatcgtt tccaccccac acaaatgggc ggtgtagaga atttcatcgg caatcggcgg
     5101 tggtgagcaa agcacaatct tgttacaacc ggcaattttt gccggcactg caagcattaa
     5161 cacggtagag aaaagcgggg ctgaaccgcc cggaatataa agccccaccg cattaatcgg
     5221 gcgagtgatg acttggcaac acacccccgg ctgggtttca atatccacct cttgattttg
     5281 ctgtgccttg tggaaagcct cgatattcgc tttcgcattt tgaatcgctt gcttaagctc
     5341 tggtgaaata cggctctgtg cttgctgaat ctgctgttct gatacaacta agctctctaa
     5401 ttttacttta tcgaactttt cagctaattc aaataatgcc ttatcgccgt tggcaattac
     5461 attcgcttta atttcattaa ctgccgcttt aatactctcg ccaaccacct gtgccggacg
     5521 ggcaagatat tgctgctgct cagtttctgt taaattgttc caaattaggg tttgcatatt
     5581 tattcctttt acgccaacat tttctcaatc ggtaaaacca atacggaact tgcgcccatt
     5641 tcttttagct gctccattgt ttcccagaac aggttttctt ggctgacaac gtgcattgcc
     5701 acacgggttt ggtcgttagc taacggtaaa atagtcgggt tttccacacc gggtaataaa
     5761 gcggtaattt ccgccagttt gtctttgggg gcgtgcagca taatatactt agattcgttc
     5821 gcctgttgca caccttgaat gcgggttaat aatttatcca ctaacgcttg tttttccgct
     5881 gaaagtggtt cttggcgttg aattaggcaa gcagttgaac ggtaaatcac ttcaacctct
     5941 tttaatccgt tcgcctctaa tgttgcacct gaagaaacca agtcacaaat cgcattcgca
     6001 attccggcac ttggagcaac ttcaaccgaa ccggtgagta aaatactttt aaacggcaca
     6061 ttttgctctt tcatataccg ctttaataaa ttggggtaag aagtggcaat cacagcatct
     6121 tctaaatctt tgatattgtt ataattttta tcacgatcaa tagctaacga taaacggcag
     6181 ccaccaaaat ctaactgacg aagtttttta taggcaacat tctcaccagc acttaatcgc
     6241 cctaattcca cttcctctaa tacattttca ccgataatcc caagatcaac tacgccgtca
     6301 aaaactaagc ctggaatgtc gtcatcacgc acccgtaaaa tttcaatcgg catattttct
     6361 gaataggcaa tcaggcgttg atcgttccaa atgattttca cgccacattg tttaagcaac
     6421 tcactacatt cttggcttaa gcgacctttt ttctgcatcg caattcttaa tcgattttgt
     6481 gttgtcattg ttgtttcctt cttatgtttt aaattgtaaa aataaaaaaa cctctcgaaa
     6541 tcacttccga gagggttaaa tttatgctgc gtttaggcgt actcggaagt cttatcttcc
     6601 gcacatacca aatgcccgaa agattattca ggtaaatgat ggtggtgatg agtacgaata
     6661 aaagccattt tgttgttctc caaactaaat tgctttaaaa gatacgctta aattttggca
     6721 atgacaagtg ttttttgcga aaaaattgct atttttcatc tctctaaaac aactatcgca
     6781 aacgtttgcg taaagcggta taatttgcct gattttttgc aaattcaaca aagtaggaat
     6841 aacactatgc aaatcagcct caaaaagatc tactccggca aagtccgtga tctctacgaa
     6901 atcgatgaaa aacgtatgct tatggttgcc accgaccgat tatctgcctt tgatgttatt
     6961 ttagacgatc ctattcctcg taaaggcgaa atcctcaccc aaatctccaa cttctggttc
     7021 aacaaattag cccacattat gccaaaccac ttcaccggcg acagtgtgtt tgacgtgtta
     7081 ccgcaagaag aggcagaagc tattcaacat cgtgcggtgg tttgcaaacg cttaataccg
     7141 gtgaaaattg aatcgattgt gcggggttat ttaaccggct ccgggctgaa agattaccaa
     7201 caaaccggca cgatctgcgg cttaacattg ccggaaggct tggttgaggc cagcaaatta
     7261 ccgcaaccga tcttcacacc gtccagcaaa gccgaggtgg gcgatcacga tattaatatc
     7321 agctacgaag agtgtgaacg ccagatcggt caagaacttg ccgccaaagt gcgtgatgcc
     7381 gctctaaaac tctacactga agcggcaaac tatgccctga ccaaaggaat tattatttgc
     7441 gacaccaaat tcgaattcgg cttagacgaa aacggtgaac taacgctaat ggacgaagtc
     7501 ctaaccccgg attccagccg tttttggtca gttgaaacct accgagaagg cacaaatcca
     7561 ccttcatttg ataaacaatt cgtgcgtgac tggctggaaa atagcggttg gaacaaacaa
     7621 cccccagccc cgaaaatacc ggcagaggta attgaaaaaa ccgtagcgaa atatcaagaa
     7681 gcattggatt tattaaccaa ataacaccac aagcggtcaa atttacgcta ttttttgcaa
     7741 aacaaaaacc tgtaagccaa cacttacagg ttttttagtt agtggtaaaa agtgcggtta
     7801 ttctaccgca cttttgcaaa attttgtgta aatctgaccg cttgttagcc ttgcgaagaa
     7861 atacgtttca tatctgtcat ataaccacgt aattcttgac cgattaactc gattgggtgg
     7921 ttacggattg cgtcattcac atcacgtaag gtaacgttgt cgatttccac tgccggagtt
     7981 ggttcaccta aatcgccttt ctctaacgtt gggataattt tctccgctaa aatcggggtt
     8041 gcaacgtgag agaataagta gttaccgtat tctgcggtat ctgaaattac cacgttcatt
     8101 tcatataaac gcttacgagc gattgtattt gcgattaacg gtaactcgtg tagagactcg
     8161 tagtaagccg attcttcata aataccggct gaaaccattg tatcaaaggc taattcaacc
     8221 cctgctttca ccattgcaac cattaacaca ccgttatcaa agtattcttg ctcagaaatt
     8281 ttaataccgt ctgcttgtgg tgcgttttca aaggctgttt tacctgtttc ttcacgccat
     8341 tttaataagt tggcatcgcc gtttgcccag tctgccatca tcgttgatga gaactcacca
     8401 ctgatgatgt cgtccatatg tttttcaaat aagaacgcta attcttcttt gatttgctca
     8461 gaaagctcga acgcacgcaa tttggcactg tttgataagc gatccatcat taaggtgata
     8521 ccgccttgtt ttaacgcttc ggtaatggtt tcccaaccgt attggatcaa tttacctgcg
     8581 taagccggat ctttaccttc cgacactaat ttgtcgtagc acacgattga acctgcttgt
     8641 aacataccgc aaagaatggt ttgttcaccc attaaatcag atttcacttc tgctacgaat
     8701 gaagattcta atacgcccgc acggtcacca ccggtagcac ttgcccacgc tttcgcaatg
     8761 gctaaacctt cgcctttagg gtcattttcc gggtgaacgg caattaaggt tggcacaccg
     8821 aaaccacgtt tgtactcttc acgcacttcc gtgcccgggc atttcggagc ggtcatcaca
     8881 accgttaaat ccggacgaat ttgctcacct tcttccacaa tgttgaaacc gtgtgaataa
     8941 ccaaatgccg caccttgttt cattaacggc attacatcgg caactacttt agagtgctgt
     9001 ttgtccggcg ttaagttaat gactaaatcc gcttcaggaa ttaattgctc gtaagtaccc
     9061 accacaaagc cgttttcaga agcacgttta aatgaagcac gtttttcagc aatcgcttcg
     9121 gcacgtaaag cgtaagcaat atctaagcct gaatcacgca tatttaaacc ttggtttaaa
     9181 ccttgagcac cacaacccac aatcacgatt tttttgcctt ttaagaaatt gcagccatct
     9241 gcaaattcat cacgatccat aaagcggcaa cgacctaact ggtctaattt ttgacgtaaa
     9301 tttaaagtgt tgaaatagtt agccattttt taatccttat ttgaattaat gagattgata
     9361 tgatgttttg tttgcacatt gttatagttt gatttgatta taccttaacc taacattgcg
     9421 taaattgaaa atattaatat ttcatattgc ataaaatgca acttgcaaaa attttgccaa
     9481 atttaaccac ttgcaatctc tacgataaag tagaaaataa ccacctaatt taatagaaga
     9541 tcaaaaaatg aaaataaaca gaatcatttt acaaagctgt agcgaatgtg ggcaacaagt
     9601 gcgagtcccg ctcacgtcaa atggcaatgc cgtttgcccg gtctgccata atatattacg
     9661 caaaagcaat acttggagct tgcaccgtag tgcttttttg gcattagcca ttttaatttt
     9721 gcttcccttc gcacttactt ttcccttgat gagcattgac ttactcggca tcaaaattaa
     9781 tgctaccgta tgggacggta tttggaagat ggcaacggag ggctaccctt acaccgcttt
     9841 tatggtgcta gtctgcgcgg taataatgcc gattgcattt gtattgctaa ttttaaccat
     9901 tcagctacaa cgtattattc gccaacgccc ccgttacacg ctgattttct taactaaaat
     9961 aaaagaatgg gtaatgcttg atgtctattt agttgctctt gcggttgccg cttttaaaat
    10021 tcgagattat gccgatctac aattcgatat caacttaatc gcctttgtgt tggtaacact
    10081 cttaaacacc ctacttttta ttaaaaccga gccaaaacaa gcatgggaac gtttttaccc
    10141 agaatatcac gccttgccgt ttgatcaccc aagcaatcca acactttgcc cgacctgtga
    10201 atattgcttt gatgaaacta ttttagactt aaaaggacgg caacgctgcc ctcgttgtga
    10261 aagcaattta tctatttccg acaacgtaaa aatacaacgg gtttgggctt gcttaattgc
    10321 agggactatt atgatgattc cggcaaatat tttcccgatt tcgacaactg aactggcagg
    10381 tcaggtttcg gcagattcat taatgtccgg cgtgcttctg tttatggaaa tgggtagcta
    10441 caccgtggca gcgattgtgt ttatcgctag tattgcggtg ccggtgagca aaattaccat
    10501 tattttttat ctcttgctgg caattcatta caagtggaaa cacccgattc attggcaaat
    10561 gaaactacta cactacgtcc attttgtcgg acgctggtca atgctggatt tattcgtgtt
    10621 atcattgatg atgtcgctac tcgaacgagg gcagattctc agtttctcgg taggggaagc
    10681 cgctttctat tttggagcag ccgtattctt aacgatgttt gcctctgcaa atttagattc
    10741 cagaatgttg tggaagattc atactgtcca gaaaacatcc taaataaaga ttttccgata
    10801 aataatacat aacatgcaaa gtaagaaaag tagtacgtaa cccaaaatct caatccctat
    10861 tgagtaagaa agagccgata atgtagcacc gatagccaga aagaaattag caaatataaa
    10921 atagtcttta ctatatttac ttgaggcaat tagggtatcc attgcagtac taaagaacat
    10981 ttccaaat
//
LOCUS       DANLRK010000084        10971 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.084, whole genome shotgun sequence.
ACCESSION   DANLRK010000084 DANLRK010000000
VERSION     DANLRK010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 10971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10971
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.084"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            6..1088
                     /gene="serC"
                     /locus_tag="PXA33_001548"
     CDS             6..1088
                     /gene="serC"
                     /locus_tag="PXA33_001548"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HDL5120870.1"
                     /translation="MTEIYNFSAGPAMMPKKVLEKAQQELLNWLDQGTSVMEVSHRGK
                     LFMELAAQSEADLRKLYNVPDNYRILFLQGGARGQFAAIPMNLIGKKGKALYLNSGHW
                     SATAAKEARNFAEIDEINILETEPQLKVSRLDFSDIAEQYDYIHYCTNETISGVEIFD
                     IPNVGNTPLVADMSSNILARGIDINKFGVIYAGAQKNLGPAGITIVIIRDDLIGNARK
                     DTPSIWNYATQRDADSMINTPPTFAWYLCSLVFQHLLAEGGLKATEERNLAKATLLYE
                     YLDSSTFYYNTVAHENRSLMNVTFTTGNDELNAKFVAEATACGLQALKGHKVLGGMRA
                     SIYNAMPIDGVKALIEFMKVFEQRNA"
     gene            complement(1145..2122)
                     /locus_tag="PXA33_001549"
     CDS             complement(1145..2122)
                     /locus_tag="PXA33_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HDL5120871.1"
                     /translation="MTLAVAAIIGGLIILVWSADRFVEGAASTARHFGMTPLLIGIVV
                     IGFGTSAPEMLVSASSALSGSPGIALGNAYGSNITNIALILGLTAIIKPLSVNSDVLK
                     KELPILLAVTLLSAYLVYDANVTQLDAIILLAVFFVYMAWTIITAMKSKNDALSLDVD
                     AELTNSAKMGLGKSILWLIIGLVSLVISSQFLVWGAVEVAKFFGVSDLVIGLTIVAVG
                     TSLPELASSIAAARKGEVDLALGNIIGSNLFNTLAVVGIAGAIEPMQVSAEVFSRDVL
                     MMSALTFLLFIFGINLYRRTEGGRINRLEGLLLFAAYVGYNFYLFKTAG"
     gene            2234..4801
                     /gene="glnD"
                     /locus_tag="PXA33_001550"
     CDS             2234..4801
                     /gene="glnD"
                     /locus_tag="PXA33_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HDL5120872.1"
                     /translation="MMKNSNELKTLLSAFTEQQRQAFQSENVLSLLAQRSQFYDQLLQ
                     ALWQDFGLAQYEHITLLAVGGYGREEMFPLSDLDILVLTEKPLEEAIQQRLNELFALL
                     WDSKLQLGTSVRTLEECIQIGKAEISVATNMLEGRFLLGNKSLWLRLKEALYQPDFWD
                     IKAFFRAKMAEKNERYARYHNTSYNLEPDLKHSPGGLRDLHLISWIMLRHYGVSSFVD
                     LLNKGLLYPEEYKELIAAQAVLFRMRFALHLQLKRYDNRLRFDRQLQLSEILGYQGEA
                     NQAVELMMRDYFQATKSISQLSQLLLNSFEQAYVNELQKNAQKQPLDQYFYRQEQQLF
                     VVERKIFKTQPTTMLDLFFHLTNDPTLIANADTLRQLRLCLQQQHEPMCFLPEMREKF
                     VQLFAQPKVISRAIVPMHQLGFLSAYLPQWKGIEGLMQFDLFHIYTVDEHTVRVMLNL
                     ENFSEDPSRAFFPLCHQLFATLSEKPLLYLAALFHDIGKGREGEHAQVGALDMREFAE
                     LHGFSQAQINDMVWLVAEHLTMSITAQRRDIHDPQIISEFAKKVQNQTALSSLLCLTV
                     ADICATSESLWNDWKASLFTQLYQFTLQQLAQNLNYQAVAQEHRLQALELMKFVLSAD
                     ERIALNAFWQPCPERYFLHHTPTQLVWHALHYVKTRRLPMVLISNEYARGGTEIFVCC
                     QDQPQLFARIAQLLSQKRISIHDAQIITTENGVVLDSFIVSERDGDDDLTDERCMQIQ
                     QAMMKMLSLTETGIKFTKKPVKHLSFKRKTRLRFLPPSSKQQTEFELFTLDREGLLAQ
                     IGYIFNRLRLNLINAKITTIGERVEDFFVVSNDQGKALSEQEQLELKAILITELDSE"
     gene            complement(4978..5736)
                     /locus_tag="PXA33_001551"
     CDS             complement(4978..5736)
                     /locus_tag="PXA33_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005567524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL5120873.1"
                     /translation="MITVKNLHHKIGNATILNNINLTIPNGGITALIGANGAGKSTLL
                     SLIARLQPIQQGEIHLNELNIANTASQTIARQLAILTQDNVIHSRITVKDLLLFGRYP
                     HHQGRITSHDNAIVEEALVRFDLSTFSHRFLNELSGGQRQRALIAMTFCQQTDHILLD
                     EPLNNLDMFHARELMKLLRKLTDELALTTIMVVHDINIAAAYADTIIAMKDGGIKMIG
                     TPKEIITTENLKTVFNLDAEVLECQGKPLVIHHI"
     gene            complement(5756..6632)
                     /locus_tag="PXA33_001552"
                     /pseudo
     CDS             complement(5756..6632)
                     /locus_tag="PXA33_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
     gene            complement(6685..7653)
                     /locus_tag="PXA33_001553"
     CDS             complement(6685..7653)
                     /locus_tag="PXA33_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDL5120874.1"
                     /translation="MFSFFRLNLFLLILLIPISASVGVADFHWSEIFHSSEQSQIFFI
                     SRLPRTLAVILVGATLAVAGMVLQIVLKNRFIEPSMIGTSQSAAIGILLASLLFPTSA
                     LLLKMSIATLSALMGMGLFMLLIRQLPPHQQLMLPLIGIVFGNVIEAISTFIAYETDS
                     LQVLSIWFSGDFSGILAGRYELLWITAILAVVVYVMADQLTIAGLGENISTNLGINYK
                     QMTWLALIVVAMITAIVVVTVGQIPFIGLVVPNIVSRIAGDRLRKNLPAVVVLGANLV
                     LICDILGRAINAPYEVPISTIFGIIGTVIFLYLLLKEKRAEEKARG"
     gene            complement(7747..8646)
                     /locus_tag="PXA33_001554"
     CDS             complement(7747..8646)
                     /locus_tag="PXA33_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005700892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL5120875.1"
                     /translation="MIKKALFALTATAVFAASAFAKDVTIPTARGEVTFKETPSKIAV
                     FDTGSLDTLQALGVKIDGMPEFKVLSYLKPSADKAVNVGTVFEPDLEALNSLNPDLII
                     VGTRTAKKFDEVSQIAKTIDMTDNGDKLIESGLQRLDSFGKLFNKEAETEKLKAEINT
                     LLDQTKAAVKGKGNGLIILVNGGKISAFGSGYRLSWIHDNLGIPMADSSIKNTGHGQP
                     VSFEFIQKLNPDWLFVLDRIAAIGQEGQSAQQVLDNELVRQTKAWKNGNIVYLSAASY
                     LAPGGYEQMKTDLTNIKTAFEKK"
     gene            8820..10283
                     /locus_tag="PXA33_001555"
     CDS             8820..10283
                     /locus_tag="PXA33_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HDL5120876.1"
                     /translation="MAEYSGTTQEKMKDKQVEQINISPHSIEAEQAVLGGIMLSNDHW
                     DNVAERVQPHDFYNYAHRIIFEQMAELVRHNKPIDIITLDQALKDKGVLQDVGGFAYL
                     AELSKNTPSAANILAYADIVRDRSDLRSVISAGNQIAEMGYKTKGRNSKEVLDEAERI
                     VFEIAEKRNNANEGPQRIDDILLKTLARMDTLSKNRHNGGVTGVSTGFVDLNKKTAGL
                     QPSDLIIVAARPSMGKTTFAMNLCENASLVDIEDKDDLDEQGNPKKKPAKPVLIFSLE
                     MPADQIMMRMLASLSRVDQTKIRTGQISDDEDTAKISSTMAILQERNNIYIDDSSGLT
                     PTELRSRARRIYRENGGLSLIMVDYLQLMRAPGFADNRTLEIAEISRSLKALAKELEV
                     PVVALSQLNRSLEQRADKRPVNSDLRESGSIEQDADLIMFIYRDEVYNDNSDLKGIAE
                     IIIGKQRNGPIGRVRLTFQGQYSRFDNYTGGHYDDEY"
     gene            10273..10578
                     /locus_tag="PXA33_001556"
     CDS             10273..10578
                     /locus_tag="PXA33_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HDL5120877.1"
                     /translation="MNTKPTQPITLCAIYRSKAKEGMYLYVPKRDQFEQVPETLRQLF
                     GKPEFVMMFNLTGEKPLVRAKNEEVLQLLIEQGYYLQMPPPPENLHKAFIAQQKGEK"
     gene            10578..>10971
                     /gene="nrdR"
                     /locus_tag="PXA33_001557"
     CDS             10578..>10971
                     /gene="nrdR"
                     /locus_tag="PXA33_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HDL5120878.1"
                     /translation="MRCPFCSTEETKVIDSRLAASGYQIRRRRECISCKERFTTFESA
                     ELVVPYVIKNNGSREPFDAEKLRTGLRRALNKRPVSADDVEKAISQIIIQLQAMGERE
                     VPSRHIGTLAMNALKQLDKVAYIRFASIY"
BASE COUNT         3560 a         2149 c         2246 g         3016 t
ORIGIN      
        1 gaaaaatgac agagatatat aacttcagtg ccggcccggc aatgatgccg aaaaaagtac
       61 tcgaaaaagc gcaacaagaa ttattaaatt ggctagacca aggcacatca gtgatggaag
      121 tcagccaccg tggaaagcta tttatggagc tggcggcaca atctgaagca gatttacgca
      181 agctgtataa tgtgccggac aattatcgta ttctatttct tcaaggtggt gcaagggggc
      241 aatttgcagc cattccaatg aatttaatcg gtaaaaaagg caaagcgctt taccttaatt
      301 cagggcattg gtcggcaaca gcagcaaaag aagcacgtaa ctttgcggaa attgatgaaa
      361 tcaatatttt agagactgaa ccgcaactta aagtgagtcg tttagatttc agcgatattg
      421 cagagcaata tgattatatc cattattgca ccaatgaaac catcagtggg gtagaaattt
      481 tcgatattcc gaatgtcgga aataccccgt tagtggcaga tatgtcgtct aatatcttag
      541 cccgagggat tgatatcaac aaatttggag taatttatgc cggagcgcaa aaaaatctag
      601 ggcctgctgg cattacgatt gtgattattc gtgatgattt aattggcaat gcacgcaaag
      661 ataccccatc tatttggaat tatgcaactc agcgagatgc tgattcgatg attaataccc
      721 cgccaacttt tgcttggtat ttatgttctt tagtgttcca acatttactg gcagagggtg
      781 gtttgaaagc tactgaagaa cgtaatcttg ctaaagctac attgctttat gagtatttgg
      841 atagttcaac attctattac aatacggtag cacacgaaaa ccgttcctta atgaatgtaa
      901 cctttaccac gggtaatgac gagctaaatg caaaatttgt ggcagaagcg accgcttgtg
      961 gtttacaggc attgaaaggc cataaagtat taggtggaat gcgtgcctca atttataacg
     1021 caatgccgat tgatggggta aaagcactca ttgaatttat gaaggtattt gaacaacgta
     1081 atgcttaatt caagcttaag aatataaatt aaagagccac gtttgtggct ctttgaattt
     1141 ggcactagcc cgcagtttta aacaaataga aattatagcc tacataagcg gcaaagagta
     1201 ataagccctc taaccgatta attctgccac cttctgtccg acgatagagg ttgatcccga
     1261 aaatgaataa taagaaggtg agagccgaca tcatcaagac atcgcgagaa aacacttccg
     1321 ctgaaacttg cattggttct atagcaccgg caatccccac cacggctaac gtattaaata
     1381 agttagaacc gataatgtta cctaaagcta aatccacttc tcctttacga gcagctgcaa
     1441 tagaagaggc taattcaggt agtgaggtgc ctaccgccac aatagtaagc ccgattacca
     1501 aatcactcac accaaaaaat ttagccactt caactgctcc ccagactaag aattgggaac
     1561 taattactag cgatactaaa ccaataatta gccaaagaat ggattttcct aatcccatct
     1621 tagcactatt ggttaattct gcatcaacat ctaagcttaa tgcatcattt ttacttttca
     1681 ttgcggtaat aattgtccac gccatatata caaaaaacac agcaagtaaa ataatcgcat
     1741 caagttgagt gacgtttgca tcataaacca aataagcaga aagcagggtg acagccagta
     1801 aaatcggcaa ctcttttttt aacacatcgg aattaactga tagtggttta attattgcgg
     1861 ttaaacctaa aatcagcgca atattggtaa tgttagaacc gtaggcatta ccgagagcga
     1921 tacccggcga tccgcttaat gcagaagatg cagatacgag catctccggt gcagatgtac
     1981 caaaaccaat gaccacaata ccgattaaca atggtgtcat accaaaatgg cgagccgtag
     2041 aggctgcccc ttccacaaaa cgatccgcac tccaaactaa tataattaag ccaccgataa
     2101 tagcggcaac agcaagtgtc ataaactatc cttttagatg aaaaacataa aggcattata
     2161 agcattttag ccaaaataaa tataggcaaa cgaaaataaa tgacctatta ttttgccagt
     2221 ttaggaagag attatgatga aaaacagcaa cgaattaaaa acgcttttat cagcatttac
     2281 ggagcaacag cggcaagcat ttcaatccga aaatgtactc agcttacttg cacaacgtag
     2341 ccaattttat gaccaactac tgcaagcact ttggcaagat tttggactcg ctcaatatga
     2401 acatattacc ctattagcgg taggggggta cggacgtgag gagatgtttc cgctttcgga
     2461 tctggatatc ttagtgttaa ctgaaaaacc gctagaagag gctattcagc agcgtttaaa
     2521 cgaactattt gccttgcttt gggacagcaa actgcaactt ggcaccagtg tgcgtacgct
     2581 cgaagaatgt attcagattg ggaaagcaga aatttcggtt gccactaata tgttggaagg
     2641 gcgattttta ttgggtaata aatcgctttg gctaagatta aaagaagcac tttatcagcc
     2701 ggatttctgg gatattaaag cgttttttag agcgaaaatg gcggaaaaaa atgagcgtta
     2761 tgcacgttat cataatacta gctataacct tgagccagat ctcaaacata gcccgggagg
     2821 attgcgagat ctgcatttaa tctcttggat tatgctacgg cattatggtg tatcttcttt
     2881 tgtcgatctc ctcaataaag ggctacttta cccggaagaa tataaggagc tgatcgcagc
     2941 acaagcggta ctttttcgga tgcgttttgc acttcatttg caactgaaac gttacgataa
     3001 tcgccttcgg tttgacagac aattacaact gagtgaaata ttaggctacc aaggcgaagc
     3061 aaatcaagcg gtggaattaa tgatgcgaga ttattttcaa gccacaaaat ctatttcgca
     3121 actgagccaa ttattgttaa acagttttga acaagcctat gtaaacgagt tgcaaaaaaa
     3181 tgcacaaaaa caaccgcttg atcagtattt ttatcggcaa gaacagcaat tgtttgtggt
     3241 cgaacgcaaa atatttaaaa cccaacccac aacaatgttg gatctctttt ttcatcttac
     3301 taatgaccct acgttaatag cgaatgccga caccttacgt cagttgagac tgtgtttaca
     3361 gcaacaacac gagcctatgt gctttttgcc tgaaatgcga gagaaattcg tacagctttt
     3421 tgctcaaccg aaagtcataa gccgagcaat agttccaatg caccaactgg gttttttaag
     3481 tgcttatcta ccgcaatgga aggggatcga agggctaatg caattcgacc tattccatat
     3541 ttatacagtc gatgagcata cggtcagagt catgttaaat ttagaaaatt ttagcgaaga
     3601 tccaagtcgg gcgtttttcc cactatgtca tcagctattt gcaacgcttt ctgagaaacc
     3661 gttgctctat cttgcggctc ttttccacga tattggcaag ggtagagagg gcgaacacgc
     3721 tcaagttggt gcgctggata tgcgagaatt tgcggaacta cacggttttt ctcaagcaca
     3781 aatcaatgat atggtttggc tggtggcaga gcatttgaca atgtcgatca ccgcacaaag
     3841 acgagatatc cacgatccgc aaatcatcag cgaatttgca aaaaaagtgc aaaatcagac
     3901 cgctttatct tcactacttt gtttaacggt tgcagatatt tgtgcaacca gtgagagcct
     3961 ctggaatgat tggaaagcct cgctgtttac ccaattatat caatttacgc tacagcaact
     4021 ggctcaaaat ttgaattatc aagcagtggc acaagagcat cgcttgcaag cattagaact
     4081 gatgaagttc gtgctttcag ctgatgagcg aatagcatta aacgcatttt ggcaaccttg
     4141 cccggagcgt tattttcttc accatacacc gactcagttg gtatggcacg ctctgcatta
     4201 tgtaaaaact cgccggttgc cgatggtttt aatcagtaat gaatatgccc gtggtggtac
     4261 agaaattttt gtatgttgcc aagatcaacc tcaactgttt gctcgaatcg cccaattact
     4321 cagccaaaaa cgcattagca ttcacgatgc acaaattatt actactgaaa atggtgtagt
     4381 gttagacagc tttattgtaa gtgagcgtga tggtgatgat gatttaaccg atgagagatg
     4441 tatgcaaatt cagcaggcga tgatgaaaat gttaagctta actgaaacag gcataaaatt
     4501 taccaaaaaa ccggtgaaac atttgtcatt taagcgtaaa actcggctgc gttttcttcc
     4561 tccgtcatca aagcaacaaa ccgaatttga actttttacc ttggatagag aagggttgtt
     4621 ggcacaaata ggctatattt ttaaccggct ccgattaaat ttaattaatg cgaaaattac
     4681 gactattggt gaaagggtag aggatttctt tgtggtcagt aatgatcaag gaaaagcttt
     4741 atcggaacaa gaacagttag aactaaaagc cattctaatt accgaactag atagtgaatg
     4801 aaaacaaccg cttggagtga agactgcaag cggtaaattt ttcaacgttt taggtaaatt
     4861 aaatcacgag ttaagagagg ttgagaattc aagaaattag aaactaaata taagtttcta
     4921 tcgtttagat taataaatta agacgcaatt tttattaatt accatgatct tcaaacgtta
     4981 aatatgatga ataactaaag gtttgccttg gcattctaac acttcggcat caagattaaa
     5041 cactgttttt aaattttcag tggtgataat ctcttttggg gtgccgatca tcttaattcc
     5101 accatctttc attgcaataa tagtatctgc gtaagcagca gcaatgttga tgtcgtgtac
     5161 caccattata gtagttaagg cgagttcatc ggttaatttt cgtaataatt tcattaattc
     5221 ccgagcatgg aacatatcta aattattcag tggttcatct aataagatat gatcggtttg
     5281 ctgacagaag gtcattgcaa ttaatgcccg ctggcgttgc cctccggata attcatttaa
     5341 gaatcggtgg gagaaagtcg ataaatcaaa tcgtaccaga gcctcttcta caatggcatt
     5401 atcgtgcgaa gtgattctgc cttgatgatg agggtaacgc ccaaataaca gcagatcttt
     5461 gacggtaata cggctatgaa tcacattatc ttgggtcaag atagccagtt gtcgagcgat
     5521 agtttgagaa gctgtgttgg ctatattcag ctcatttaag tggatttcac cctgttggat
     5581 aggttgcaag cgtgcaatta aagagagcag ggtggatttg cccgcaccat ttgctccgat
     5641 taaagcggta ataccaccat ttggaatggt taaattgata ttattcagaa tcgtggcatt
     5701 cccgattttg tggtgaagat ttttaaccgt tatcattttc ttccttaatt tatggttatt
     5761 ttttccgttt taaaatgaga taaataaaga caataccgcc aataaattca ataacgacac
     5821 tcagaacccc ttgcattttc agcacttgct cgaagattgc ctgccctaaa attaaggtga
     5881 tggcagagat taaaaaagtc attggtattc gcacgctatg gcaaacagtc gggctaattg
     5941 cattaacaat ggcacaaaca agtagcccta gaaatagaat cgggccaaca agtgcggttg
     6001 aaacagacac caacaatcca caacagatta gtaattgacg tgaaaattga tgatattcta
     6061 acccaagccc aattgcctta tctttgccaa gtaacaaaat atctaattta tgccgttgta
     6121 accagaccca aatggcagag agaatagcga tacagcctcc aatccaaagt aatgtggggg
     6181 tagtcaggtt aaatgaggca aagaagagcc ttgagcaacg gcaaactctg ttgggtcgac
     6241 attcgttgca acaaattatt taggcttcta aataagacac caaagatgac accgattaaa
     6301 agcattcggg ttaaatcgga tcgatttcga ttcagtgaac taaatagtaa tattgatgcc
     6361 cctagcatta acaaggtttc aaagaaaaac ttatagcttg ggtctaactg agtaaagcct
     6421 aaaccgccaa ataaaaatac taacgaggtt tgcaatagca agtagagcga atcgaacccc
     6481 aaaatacttg gagttaaaat aggattatgt gtgagtgttt ggaatagcag ggtcgaaata
     6541 ccaatcgtat aagcaatagt acagagcaaa ataagctttt tgccacgcag ttgcaatata
     6601 aagtcccaat tacctttcgc attataggtc atataaaaca gaatacttgc taataagatt
     6661 aagcttaaaa cgataaagcg tgatctaacc acgagccttc tcctcagccc gtttctcttt
     6721 gagcaataaa tagaggaaaa tcactgtccc aataatccca aaaatagtag aaatcggcac
     6781 ttcataaggg gcattgatgg ctcgccctaa aatgtcacaa atcaggacta aattcgcacc
     6841 taacaccacg accgcaggca gatttttacg caatcggtca cctgctatgc gagagacaat
     6901 attagggacg accaagccga taaacggaat ctgtccaacc gtcaccacga caatagcggt
     6961 aatcatcgcc accacaatta aggcaagcca agtcatttgt ttgtagttaa tgcctaagtt
     7021 ggtactaata ttttcaccaa ggcctgcaat ggttaattga tctgccatca cataaaccac
     7081 aacagccaga atagcggtaa tccagagcag ttcataccga cctgccaaaa taccggagaa
     7141 gtcgccggaa aaccaaatcg aaagcacttg taggctgtca gtttcataag caataaaagt
     7201 gctgatagct tcaataacat tgccaaaaac gatcccgatt aatgggagca ttaattgttg
     7261 atgaggcggt aattgacgaa tgagcaacat aaaaagcccc attcccatta atgcacttaa
     7321 tgtggcaata gacattttta acagtaatgc tgatgttggg aaaagtaaac tagcaagtaa
     7381 aataccaatt gccgcacttt ggctagtgcc aatcatacta ggctcaataa agcggttctt
     7441 cagcacaatt tgtaatacca tacctgcaac ggctaaggtt gcaccgacta atatcactgc
     7501 gagcgttctt ggtaaacggc tgataaaaaa gatctgactt tgttcggaag aatgaaaaat
     7561 ttcactccaa tgaaaatctg ccacacccac actggcactg attggaatga gcaatattaa
     7621 aagaaataga tttaatctaa aaaaagagaa catagtgata aatagcaagc ggtcatattc
     7681 tctctctttt ttacaaatgc aggcacttgc aaaaatagga ggaaatctga ccgcttgtaa
     7741 ggttaattat tttttctcaa atgctgtttt aatgttagtt aggtcggttt tcatctgctc
     7801 ataaccaccc ggcgctaaat atgaggcagc acttagataa acgatattac cgtttttcca
     7861 agctttagtt tgacgaacta attcattatc caacacttgt tgggctgatt gaccttcttg
     7921 tccgatagcc gcaatacggt ctaacacaaa caaccaatcc ggattgagtt tttggataaa
     7981 ttcaaacgaa acaggctgac cgtgaccggt atttttaatg ctagaatctg ccattggaat
     8041 ccctaaatta tcgtgaatcc aacttaaacg ataacctgaa ccaaatgcag aaattttgcc
     8101 accattgact aaaataatca gtccgttacc tttacctttt actgctgctt tggtttggtc
     8161 taataaagta ttaatttctg cttttagttt ttcggtttcc gcttctttat taaataattt
     8221 gccgaaagaa tcaagacgct gtaatccgct ttcgattaat ttatcgccat tatccgtcat
     8281 gtcgatggtt ttcgcaattt ggctgacttc atcaaatttt tttgctgtgc gtgtgccaac
     8341 aatgatcaaa tccggattta aagaatttaa cgcttctaaa tccggctcaa ataccgtacc
     8401 gacattcacc gctttgtctg cactcggttt aagataagaa agcaccttaa attcaggcat
     8461 accgtcaatt tttacaccta aggcttgtaa agtatccaaa cttccggtgt caaacacggc
     8521 aattttagac ggggtttctt tgaaagtaac ctctccacgt gcagttggaa tagtaacatc
     8581 tttggcaaat gcagaagcag caaacacagc agttgcggtc aatgcaaaca aggctttttt
     8641 aatcattgga taactccatt tatattgaaa ataattctca aagattttaa cgaaaattga
     8701 ttttaagtaa agcggaaaat aacaagcggg cgaattttgc ctaaaatttg caaatttcaa
     8761 tttaattaaa attccgatac actatgtaca tcaatacgtc attatagcaa gagaaaaata
     8821 tggcagaata tagcggaaca acccaagaga agatgaaaga taagcaggtt gagcaaatta
     8881 atatctcacc acattcgatt gaggcagagc aagcggtgct tggtggcatt atgctcagta
     8941 atgatcactg ggataatgtt gctgagcgtg tacaaccgca cgatttttac aactatgcgc
     9001 atcgaattat ttttgaacaa atggcggagt tggtgcgcca caataaaccg attgatatta
     9061 ttacgctgga tcaagcctta aaagacaaag gggtgctgca agatgtagga ggttttgcct
     9121 atttagccga gctttctaaa aataccccga gtgcagcaaa tattttagcc tatgcggata
     9181 ttgtgcgaga tcgttccgat ttaagaagtg taatcagtgc cggcaatcaa attgcggaaa
     9241 tgggctacaa aacgaaaggg cgtaactcca aagaagtgtt agacgaggca gagcgaattg
     9301 tgtttgaaat tgcagaaaaa cgcaataacg ccaatgaagg tccacaacgt attgacgata
     9361 ttttgcttaa aacactcgct cgaatggata cgctatctaa aaatcgccat aacggcggag
     9421 ttactggtgt ttctaccggg tttgttgatc tcaataaaaa aaccgcaggg ttacagcctt
     9481 cggatttaat cattgtggca gcccgtcctt caatggggaa aactactttt gcaatgaact
     9541 tatgtgaaaa tgcctcatta gtggatattg aagataaaga tgatttagac gaacagggta
     9601 atccgaagaa aaaaccggca aagccagtgc taattttcag cctggagatg ccggcagacc
     9661 aaattatgat gcgtatgctt gcctcacttt ctcgggtcga tcaaaccaag atccgaaccg
     9721 gtcagatttc agatgatgaa gacactgcta aaatctccag cacaatggca attttacaag
     9781 aacgtaataa tatctatatt gatgatagct ccggtttaac accgaccgaa ttacgttctc
     9841 gagcgagacg gatttatcgt gaaaatggcg gattaagctt gattatggtg gactatctcc
     9901 aattaatgcg agcgccggga tttgcagata atcgtacttt agaaatcgca gaaatttccc
     9961 gttctttaaa ggcactagcg aaagaattag aagtgccagt agtcgctctt tctcagctaa
    10021 accggagttt ggaacaacgt gccgataaac gtccggtaaa ctcggatttg cgtgagtcag
    10081 gttctattga gcaagatgca gacttaatta tgtttattta tcgtgatgag gtctataacg
    10141 acaattccga tctgaaaggc attgcggaaa ttattatcgg aaaacaacgt aacggcccga
    10201 ttggtcgggt gcgtttaacg ttccaagggc agtattcccg ttttgataat tacacaggag
    10261 ggcattatga tgatgaatac taaaccaacc cagccaatca cactttgtgc gatttatcgc
    10321 agtaaggcaa aagaaggaat gtatttatat gtgcctaagc gtgaccaatt tgagcaagtg
    10381 ccggaaacat tacgccagtt atttggtaag cctgaatttg tgatgatgtt taatttaacc
    10441 ggtgaaaagc cattggtgcg tgctaaaaac gaagaagtgt tacaattact tatagagcaa
    10501 ggctactatt tgcaaatgcc acctccgccg gagaatttgc ataaagcctt tatcgcacaa
    10561 cagaaagggg aaaaataatg cgctgcccat tttgttccac tgaagaaact aaagtgatcg
    10621 attcacggct tgcagcaagc ggttatcaaa ttcgccgccg ccgtgaatgt ataagctgta
    10681 aagaacgttt tactaccttt gaatcggccg aattagttgt gccttatgtg atcaaaaata
    10741 atggtagccg agagccgttt gatgccgaaa aattaagaac agggttaaga cgtgcattaa
    10801 ataaacgccc tgtcagtgct gatgatgtcg aaaaagcaat tagccaaatc attattcagc
    10861 tgcaagcgat gggcgaacga gaagtgccga gtcgccatat cggtacattg gcaatgaacg
    10921 cacttaaaca attagacaaa gtagcatata tccgctttgc ctctatttat t
//
LOCUS       DANLRK010000085        10954 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.085, whole genome shotgun sequence.
ACCESSION   DANLRK010000085 DANLRK010000000
VERSION     DANLRK010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 10954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10954
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.085"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            72..1106
                     /gene="metN"
                     /locus_tag="PXA33_001558"
     CDS             72..1106
                     /gene="metN"
                     /locus_tag="PXA33_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HDL5120879.1"
                     /translation="MIQLKNISKQFEVKGKKITALDNVNLEVPRGTIYGVIGASGAGK
                     STLIRCVNLLERPTIGQVIVDGQDLTAMSDKELIHARRNIAMIFQHFNLLSSQTVFEN
                     VALSLRLSNTPKEQVEKKVNELLDLVGLTDRKDVYPANLSGGQKQRVAIARALASDPH
                     VLLCDEATSALDPATTQSILQLLKDINKRLGLTILLITHEMEVVKRICDRVAVIDKGQ
                     LIESGSVSEIFSNPKTELAQKFIQSTFHFELPQEYTEQLSSSPTEHSKPIIKFEFTGR
                     SVDAPLLSMASKKFGIDFSILMSQIDYAGGVKFGFVIAEVSGNPDSIAEAKFYLIDNN
                     VKVEVLGYVG"
     gene            1096..1773
                     /locus_tag="PXA33_001559"
     CDS             1096..1773
                     /locus_tag="PXA33_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL5120880.1"
                     /translation="MWADFMQEMTPQMWSLVWESTFETVYMGFVATALAVVIGLPIGF
                     LAFLTGKGEILENPRLHQVLDVVINIGRSIPFIILLIILLPFTRLVVGTTLGTTAAIV
                     PLSVSAIPFFARLTSNALLEIPSGLTEAAKSMGATNWQVVTKFYLPESLPILINGITL
                     TLVALIGYSAMAGAVGGGGLGNLAISYGEHRNMVYVKWIATIIIVAIVMISQKLGDHL
                     AKRYDHR"
     gene            1796..2629
                     /locus_tag="PXA33_001560"
     CDS             1796..2629
                     /locus_tag="PXA33_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family lipoprotein"
                     /protein_id="HDL5120881.1"
                     /translation="MSFKKILGVALVSALALTACKEEKKAESTAAPAAQAPAKIKVGV
                     MSGPEHTVAERAAQIAKEKYGLEVEFVLFNDYALPNTAVSKGDLDANAFQHKPYLDKD
                     SQSKGLNNLVIVGNTFVYPLAGYSKKVKNVSELAEGAVIAVPNDPSNLARALILLEKQ
                     GLIKLKDNTNLFSTSVDIIENPKNLKIKEVDTSIAAKALDDVDLAVVNNTYAGQVGLN
                     TQDHGVFVESKDSPYVNIIVARQDNKDAANVQNFIKSYQTEEVYQEAQKHFKDGVVKG
                     W"
     gene            2712..3542
                     /locus_tag="PXA33_001561"
     CDS             2712..3542
                     /locus_tag="PXA33_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family lipoprotein"
                     /protein_id="HDL5120882.1"
                     /translation="MNFKKLLGVALVSALALTACKDEKAQAPATTAKTENKAPLKVGV
                     MTGPEAQMTEVAVKIAKEKYGLDVELVQFTEYTQPNAALHSKDLDANAFQTVPYLEQE
                     VKDRGYKLAIIGNTLVWPIAAYSKKIKNISELKDGATVAIPNNASNTARALLLLQAHG
                     LLKLKDPKNVFATENDIIENPKNIKIVQADTSLLTRMLDDVELAVINNTYAGQAGLSP
                     DKDGIIVESKDSPYVNLVVSREDNKDDPRLQTFVKSFQTEEVFQEALKLFNGGVVKGW
                     "
     gene            3594..4385
                     /gene="metQ"
                     /locus_tag="PXA33_001562"
     CDS             3594..4385
                     /gene="metQ"
                     /locus_tag="PXA33_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HDL5120883.1"
                     /translation="MKIMKLAGAVAIFSLFLTACNDKAEKLKVGVISGPEHKVMEVAA
                     KIAKEKYNRDVELVVFTDYATPNAALDKGDLDLNAFQHKPYLDNQIQEKGYKLVPVGN
                     TFVYPIAAYSKKIKSLAELKDGDTIAVPNDPTNLARALILLEKQDLIKLRADAGLKAT
                     SVDIIENPRKLVIQEIEAPLLPRTLDDVAFSIINTTYAGQNGLTPTKDGIFVEDKDSP
                     YVNLIVARENNQHSEAVKDLVKAYQTEEVYNKANEEFKGTMIKGW"
     gene            complement(4430..5581)
                     /gene="metK"
                     /locus_tag="PXA33_001563"
     CDS             complement(4430..5581)
                     /gene="metK"
                     /locus_tag="PXA33_001563"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018356717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HDL5120884.1"
                     /translation="MAINLFTSESVSEGHPDKIADQISDAVLDEILKQDPKARVACET
                     YVKTGMALVGGEITTSAWVDIENLTRQVICDIGYTHSDMGFDAHSCAVLNAIGKQSPD
                     INQGVDRADPLEQGAGDQGIMFGYATNETEVLMPAPITYAHRLMEQQSKVRKSGKLDW
                     LRPDAKSQLTFAYENNKIIGIDAVVLSTQHAEHISQKELIEGVMEEIIKPVLPSEWLS
                     QNTKYFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGAARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQLSYAIGVADPTSIMVETFGTGKVSNEILVKLIYQNF
                     DLRPYGLIKMLDLIKPIYRETAAYGHFGRKHFPWEKTDKAEALRAGAGL"
     gene            complement(5764..6606)
                     /locus_tag="PXA33_001564"
     CDS             complement(5764..6606)
                     /locus_tag="PXA33_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HDL5120885.1"
                     /translation="MKVGLVLEGGGLRGIFTAGVLDVFLDEHIHIDGAIGVSAGALFG
                     VNLPSKQKGRALRYNKKYAKDKRYMGLHSLLTTGNIVNRDFAYYELPSKLDPFDQQAF
                     EDSGMDFWVTVTNVETGEPEYIKIDNVFEQMEAFRATSSMPIVSKIVEIEGKKYLDGG
                     VSDSIPVQKFIEMGYDKIIVVLTRPLEYRKKPSSMWLFKRFYKKYPKLVQRWENRYAE
                     YNQAVEQIIQLNEKQQIFVIRPSRTVKISRLETDVNKIQAMYDLGVEDAKNALAGLRA
                     YLAK"
     gene            6666..6983
                     /locus_tag="PXA33_001565"
     CDS             6666..6983
                     /locus_tag="PXA33_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HDL5120886.1"
                     /translation="MADAEFMDFLLDQLSPISGLRSKKMFGEYCLFFDNKIVAIISKD
                     YRIFVKANPQTLPLFLAENAEQFHYFAKGKVQKMHYWSIPELAIEEADELVKWIRLGL
                     QAV"
     gene            7074..7787
                     /locus_tag="PXA33_001566"
     CDS             7074..7787
                     /locus_tag="PXA33_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease"
                     /protein_id="HDL5120887.1"
                     /translation="MNQKKLVSLIVVIVLAIISQIFSFFDKKEPSKAQEKSTQSSQSH
                     QVSTSSLGNYDVQMKNDRLKQHIVDTDYYTLALSWSPAFCQKQKRNNHGKIPSHLEYQ
                     CGGSADFGWVIHGLWPQSGKARSVDEHPRYCQGDLPMVAEQTIKQYLPESPGATLLQG
                     EWEKHGACAFNQADDYFAKQKELFNQLKLPDTEMSKLALFNWMRANNPHLQNVYLGGS
                     KDELYICYDKAFQPMDCPR"
     gene            7787..8281
                     /locus_tag="PXA33_001567"
     CDS             7787..8281
                     /locus_tag="PXA33_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HDL5120888.1"
                     /translation="MADLRQLKMQVQRQLKRDLERANHYFNTTFTPPSISYAVRGVKA
                     GVAYLERNEVRFNPVLLQENEQAFIRQVVPHELAHILVYQHFGRVQSHGKEWKMMMET
                     VLGVPAEIYHCFDTQSVQQQFSYQCACQTHQLSIRRHNAVMRDKKSYICRLCKTQLHF
                     VEKS"
     gene            8386..9666
                     /locus_tag="PXA33_001568"
     CDS             8386..9666
                     /locus_tag="PXA33_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005693148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL5120889.1"
                     /translation="METIVIVGGGAGGLELATYLGNKLGRKQKAKVVLIDRNATHLWK
                     PLLHEVATGTIDEGTDALSYRAHAKNHSFEFQQGTLVAVDREKKTVSLAPIYNDQNQL
                     LVKERHISYDKLVIAIGSKSNDFGTKGVAENCIFLDGSEQAKDFQKRMMELFLRFSHS
                     ENKDVKIAIVGGGATGIELSAELYHVVKNLNSYGFGKLNRASLKVTLIEAGPRLIPAL
                     PEKVSVSAFHELKKAGVSVRLGTMITEARPDGLMTRLGEKIEADIMVWAAGVKAPEIT
                     REFGFETNRLNQIEIKDTLQTTVDESVFVIGDCASLIQDGKPIPPRAQAAHQMATRCG
                     KNIVALIEGKEMKPFRFNDKGSLLSFSKFGTVGNLMGNLVSGDMFIEGKIARLAYLSL
                     YRMHQVALHGCVKTGLIVLVGQINRFLRPTMKLH"
     gene            complement(9753..10790)
                     /locus_tag="PXA33_001569"
     CDS             complement(9753..10790)
                     /locus_tag="PXA33_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL5120890.1"
                     /translation="MNVLKLISLKSILIASGIFLSAQAAQAEGRLTVYCSVQNNVCEE
                     MTKQFSHKYNVETKFIHGGTGTIFGKVKAEASNPQADIWYGGTIEPHFQAGELGLLEA
                     YRSPKQAEILPQFKSLVESEQGKFTSIVYMLVLGLGVNTEKLAKLGIEAPKKWEDLSD
                     PRLKGEVQLPDPRSSGTTYTFMTTLSTLWGEEKTFDYLKKLNNNVSQYVKSTLVTSNL
                     ARGESAVTVGFVHSYATEKEKGAPIEAIIPEGKVGYALGGVSIIKNARNLDNAKLFMD
                     WVLSKEAQEIPWKKHGVYQTPTNINAEVAPQSTKAEALDFVEIDYQKAGSSEEGKRLI
                     DKWLAEIKLAN"
BASE COUNT         3288 a         2051 c         2364 g         3251 t
ORIGIN      
        1 tgattgacat agcattctag acgtctaaaa taaccaaaaa taatagttaa ttgaatgtgg
       61 ataaatagaa aatgattcag ctgaaaaata tcagcaaaca atttgaggta aagggcaaaa
      121 aaattaccgc tctggataat gtaaacctag aagtaccaag aggcactatt tatggtgtca
      181 ttggggcatc aggggcagga aaaagtacct taattcgctg tgtaaattta ttagaacgcc
      241 caacaatcgg gcaagtgatt gtcgatgggc aagatttaac ggcaatgtcg gataaagagt
      301 tgattcacgc tcgccgtaat attgcaatga ttttccaaca ttttaatttg ctttcatcac
      361 aaactgtatt tgaaaacgtg gcgttatcgt tgcgtttaag caatacgcca aaagagcaag
      421 ttgagaagaa agtcaatgag ctgttggatt tagtggggtt aacagatcgt aaagatgttt
      481 accctgcaaa tctttccggt gggcagaaac agcgtgtagc gattgctcgt gcgttagcca
      541 gcgatccaca cgttttgttg tgtgatgaag cgaccagtgc gttagatccg gcgactacgc
      601 aatccatttt gcagctatta aaagatatta ataaacgtct tggtttgacg attttattaa
      661 tcactcacga aatggaagtg gtaaaacgca tttgtgaccg agtagcagtg attgataaag
      721 ggcagttgat tgaatctggc tcggtaagtg aaattttctc gaatccgaaa accgaattag
      781 ctcagaaatt tattcaatcg actttccatt ttgaattacc gcaagaatat accgagcagc
      841 tttcaagttc gccgactgaa catagtaagc cgattattaa atttgagttt accggacgtt
      901 cggtggatgc acctttgctt tcaatggcat cgaagaaatt cggcattgat tttagtattt
      961 tgatgtcgca aattgattat gcaggcgggg tcaaatttgg atttgtaatt gcagaagtga
     1021 gtggtaatcc ggattccatt gcagaggcaa aattttattt aattgataac aatgtaaaag
     1081 tagaggtgtt aggctatgtg ggctgatttt atgcaggaga tgacgccaca gatgtggagc
     1141 ttagtatggg aatctacttt tgaaaccgtt tatatgggct ttgtggcaac ggcattggcg
     1201 gtagtcatcg gtttaccgat tggtttcttg gcgtttttaa ccggtaaagg cgaaatttta
     1261 gaaaatccga gattacacca agtgttagat gtcgttatta acatcgggcg ttcgattccg
     1321 ttcattattt tattgattat tttgttgcca tttactcgct tagttgtggg cacaacatta
     1381 ggcacaacgg cagcgattgt tcctttaagt gtttcggcca ttccgttttt tgcccgttta
     1441 acttcaaatg cgttgcttga aattccgtcc ggtttaacag aagcggcaaa atcaatggga
     1501 gcaacaaatt ggcaggtagt gacaaaattt tatttaccgg agtcattacc gattttgatt
     1561 aatggcatta cgctaacgct ggttgcttta attggctact cagcaatggc gggggctgtt
     1621 ggtggcggcg gtttaggcaa tttagcgatt agctatggcg aacaccgtaa tatggtgtat
     1681 gtgaaatgga ttgctaccat tattattgtt gcgattgtga tgattagcca aaaattgggt
     1741 gatcatttag caaaacgtta cgatcatcgt taattttatt taaaaagagg tttctatgag
     1801 tttcaagaaa attttaggcg ttgcattggt ttctgcatta gcattaaccg cttgtaaaga
     1861 agagaaaaag gcagaatcta ccgctgctcc ggcagctcaa gctccggcta aaatcaaagt
     1921 aggggtaatg tcaggccctg agcataccgt tgcagaacgt gcagcacaga ttgcaaaaga
     1981 aaaatatggt ttagaagttg agttcgtttt atttaacgac tacgctttac cgaatactgc
     2041 agtaagtaaa ggtgatttag atgcaaacgc attccaacac aaaccatatt tagataaaga
     2101 cagccaatca aaaggtttaa acaacttagt gattgtgggt aacacctttg tttatccgtt
     2161 agcgggctac tctaaaaaag tgaaaaatgt gtctgagtta gcagaaggtg cggtaattgc
     2221 agtaccaaat gatccttcaa acttagctcg tgctttgatt ttattagaaa aacaggggtt
     2281 aattaaatta aaagataata ccaatttatt ctcaacttca gtggatatta tcgaaaatcc
     2341 gaaaaactta aaaattaaag aagtggatac ttcaattgcc gcaaaagcgt tagatgacgt
     2401 agatttagcg gtggtaaata acacttatgc cgggcaggta ggcttaaata cccaagatca
     2461 cggtgtattt gttgagtcta aagattcacc gtatgtgaat attattgtgg ctcgccaaga
     2521 caataaagat gcagctaatg tacaaaactt tattaaatct taccaaaccg aagaagtgta
     2581 ccaagaagca caaaaacact ttaaagatgg tgtagtaaaa ggttggtaat ttagctgatt
     2641 tttacttaaa tggcgattaa gcgctaggtt aatcgccatt ttcatcttta aaatatagga
     2701 aggagcaaac aatgaacttt aaaaaattat taggtgtggc gttagtatct gccttagcac
     2761 ttactgcgtg taaagatgaa aaagcacaag cacctgctac aacagctaaa actgaaaaca
     2821 aagccccatt aaaagtgggt gtgatgaccg gccctgaagc ccaaatgacc gaagtggcag
     2881 tgaaaattgc aaaagagaaa tatggcttag atgtagagtt agtgcagttt actgaataca
     2941 ctcaaccaaa tgccgcactt cattctaaag atttagatgc taacgcgttc caaacggtgc
     3001 cttatttaga gcaagaagtg aaagatcgtg gttataaatt agcgattatc ggcaatacgc
     3061 tagtatggcc aatcgcggct tattctaaaa aaattaaaaa catttccgag ttaaaagacg
     3121 gagcgactgt tgcgattcca aacaatgcaa gtaatactgc tcgtgcgtta ttattgcttc
     3181 aagctcacgg tttattgaaa ttaaaagatc cgaaaaatgt gtttgctacc gaaaacgata
     3241 ttatcgaaaa cccgaaaaat atcaaaatcg tacaggcgga tacctcactt ttaacccgta
     3301 tgttagatga tgtagaactt gcggtaatca acaacactta cgcaggtcaa gctgggttaa
     3361 gtccggataa agacggtatt attgtggaat ctaaagattc accgtatgtg aatttagtgg
     3421 taagtcgtga agataataaa gatgacccac gcttacaaac ttttgtgaag tcattccaaa
     3481 ccgaagaagt attccaagaa gcgttaaaat tatttaacgg tggtgtggtg aaaggttggt
     3541 aatcagttgg acggcatatt ctgccgtcct tttcttaaaa ggagaaacag ataatgaaaa
     3601 taatgaaatt agccggtgca gttgcaattt tctcgctttt tttaaccgct tgtaatgata
     3661 aagccgaaaa gttgaaagtc ggtgtgattt ccggccctga acataaggta atggaagtgg
     3721 cggcaaaaat tgcaaaggaa aaatataacc gtgatgttga attagtggta tttaccgatt
     3781 atgccacgcc taatgcagct ttagataaag gcgatcttga tttgaatgct ttccagcata
     3841 aaccttattt agataaccaa attcaggaaa aaggctataa attagtgccg gtcggcaata
     3901 cttttgttta tccgattgcg gcttattcca aaaaaattaa atcgctggca gagttgaaag
     3961 atggtgatac tattgcagtg cctaatgatc cgactaattt agcccgtgct ttaattttat
     4021 tggaaaaaca agatttaatt aagctgcgag cagatgcagg cttaaaagca accagtgtgg
     4081 atattattga aaaccctcgt aaattggtga tccaagaaat tgaagcacca ttattgcctc
     4141 gaacgttgga cgatgttgcc ttttcgatta ttaatactac ctacgcaggg caaaacggtt
     4201 taacgccaac caaagacgga atattcgttg aagacaagga ttcgccttat gtgaatttaa
     4261 ttgttgcccg tgaaaataac caacattctg aagccgtaaa agatttggtg aaagcctacc
     4321 aaacagaaga ggtgtataac aaagcgaatg aagagtttaa agggacgatg ataaaaggct
     4381 ggtaattgaa gataaataag gcatagattc ctatgcctta ctttttatgt tacagacctg
     4441 ctccggctct taatgcttcg gctttgtcag ttttttccca aggaaaatgt ttacgaccga
     4501 agtgtccgta ggcagcggtt tcacggtaga ttggtttgat taaatctaac attttgatta
     4561 agccatacgg gcgtaagtca aagttttggt agattaattt cacaagtatt tcgttgctca
     4621 ctttacctgt gccgaaggtt tcgaccataa ttgatgttgg gtcagccacc ccgatagcat
     4681 aagaaagttg gatctcacag cgatcggcta agcctgcggc aacgatattt tttgcgacat
     4741 aacgagcagc ataagcagca gagcgatcca cttttgacgg gtctttacca gagaatgcac
     4801 caccaccgtg gcgagccgca ccaccgtagg tatctacaat gattttacgc ccggttaaac
     4861 cacagtcacc cattggaccg ccaatcacaa aacgaccggt tgggttgatg aaatatttgg
     4921 tgttttggct gagccattcg cttggtagta ccggtttgat gatctcttcc atcacccctt
     4981 caattaactc tttttgcgaa atatgctctg cgtgttgtgt agaaagcact acggcatcaa
     5041 tgccgataat tttattgttc tcataagcaa atgttaattg gcttttagcg tcagggcgta
     5101 accaatcgag tttgcccgat ttacgcactt ttgactgctg ttccattaag cggtgtgcgt
     5161 aagtaattgg agccggcatt aacacctcgg tttcattggt agcgtagcca aacataatcc
     5221 cttggtcgcc cgcaccttgc tctaacggat cggcacggtc aacgccctgg ttgatgtccg
     5281 gagattgttt accaatcgca tttaataccg cacaagagtg agcatcaaag cccatatcag
     5341 agtgagtata gccgatgtcg caaatcactt ggcgggttaa gttttcaata tccacccacg
     5401 cagaggtagt gatttctccc cctactaatg ccataccggt tttcacataa gtttcacaag
     5461 caacgcgtgc tttagggtct tgttttaaaa tttcatctaa taccgcgtct gaaatttggt
     5521 ctgcgatttt atccggatgc ccttctgaaa ccgattcaga agtaaataaa ttaattgcca
     5581 tttgtattcc tctctatctt aaatatggat tattaatagt aggggcgggt ccagtgcccg
     5641 cccataaatt atataaaatg tttggacagg cacaggaccg gtccctataa aatttgtttg
     5701 gaagtgtttc cgtctagacg tctataatat attttttgaa cgttttaggc aaatagaatt
     5761 tgtttatttg gctaaatatg cccttaaacc agccaacgca tttttggcat cttctacccc
     5821 taaatcgtac attgcttgta ttttatttac atccgtttca aggcgactga ttttgactgt
     5881 tctgcttgga cgaatcacaa aaatctgttg tttttcattg agttgaataa tctgctcaac
     5941 ggcttgatta tattccgcat agcggttttc ccagcgttgc actaatttcg gatatttttt
     6001 ataaaaacgt ttaaacagcc acatcgagct tggtttctta cggtattcaa gtgggcgagt
     6061 aagcaccacg ataattttgt catatcccat ttcaataaat ttctgcaccg gaatactgtc
     6121 tgacacacca ccgtctaaat attttttacc ctcaatttcg actattttgg atacaatcgg
     6181 cattgaggaa gtagctcgaa aggcttccat ttgctcgaac acattgtcaa ttttgatgta
     6241 ttcgggctca ccggtttcga cattggttac agtaacccaa aagtccatac cggaatcctc
     6301 aaaggcttgt tggtcaaacg gatccagttt ggaagggagt tcatagtaag caaaatcccg
     6361 attaacgata ttgccggtgg taagcaagct gtgtaacccc atataccgct tatctttagc
     6421 gtattttttg ttgtatcgca acgccctacc tttttgtttt gaaggcaaat tcacgccaaa
     6481 taacgcaccg gctgaaacac cgattgctcc atctatatga atatgttcat ctaaaaatac
     6541 atctaacacg ccggcagtaa aaatacctcg caaaccaccg ccttccagca ctaatccaac
     6601 tttcatatca ttctccctgt aatttgtgta ttataacccg aattatcttg ctaggagaaa
     6661 tcacaatggc tgatgcagaa tttatggatt tcctgttaga tcaactaagc ccgatttccg
     6721 gtttgcgttc gaaaaaaatg tttggtgaat attgcttgtt ttttgataat aaaattgtcg
     6781 ctattatcag caaagattat cgcatttttg tcaaagctaa tccgcaaacc ttgccgttat
     6841 tcttagctga aaatgccgaa caatttcact attttgctaa aggaaaagtg caaaaaatgc
     6901 actactggag cattccggaa ttagcgattg aagaagccga tgaactggtc aaatggattc
     6961 ggctaggctt acaagcggtg taatttagcg aaaattttgt aaaaaaacgc taaaatatgc
     7021 ccgcttgtta ggggggggga cctaatattt ctatacttta agattttata tttatgaacc
     7081 aaaaaaaatt agtttctttg attgtcgtta ttgtgttggc aatcatttct caaattttta
     7141 gcttttttga taaaaaagag ccttcaaaag cacaagaaaa atcgacccag agtagtcagt
     7201 ctcatcaagt ttcaacttca tcactaggga actatgatgt gcagatgaaa aacgaccgtt
     7261 tgaaacagca tattgtagat accgattatt acaccttagc gttgtcgtgg tcgccggcgt
     7321 tttgccaaaa acaaaagcga aataatcacg gtaaaattcc gtctcatttg gaatatcaat
     7381 gtggtggctc agcggatttt ggttgggtta ttcacggctt atggccgcaa agtggcaagg
     7441 caagaagtgt tgatgaacac cctcgttatt gccaaggcga tttgccgatg gtggcagagc
     7501 agacgattaa gcaatattta cctgaatcgc cgggggcaac tttgttgcaa ggcgaatggg
     7561 aaaaacacgg tgcttgtgcc tttaaccaag cggatgatta cttcgccaaa caaaaagagc
     7621 tatttaatca gctcaaatta ccggataccg aaatgtctaa attagcactg tttaactgga
     7681 tgcgagccaa taaccctcat ttgcaaaatg tctatttagg gggtagcaaa gatgagctgt
     7741 atatttgcta tgataaagca tttcaaccaa tggattgccc acgctaatgg cagatcttcg
     7801 ccaactgaaa atgcaggttc agcgccaatt aaagcgtgat cttgaacgtg caaatcacta
     7861 ttttaatacc acttttacgc caccaagtat tagctatgcg gtgcgtggcg taaaagccgg
     7921 tgtggcttat cttgaacgca acgaagtacg gtttaatcct gtgttattgc aagaaaatga
     7981 acaggcattt atccgccaag ttgtgccgca cgaactggct catatcttgg tttatcaaca
     8041 ttttgggcgg gtacaatctc atggtaaaga gtggaaaatg atgatggaaa ccgtactggg
     8101 tgtgccggcg gaaatttacc attgttttga tacccaaagt gtgcaacagc aatttagtta
     8161 ccaatgtgcc tgccagactc accagctttc gattcgccga cacaatgccg tgatgcgaga
     8221 taaaaaaagc tatatttgcc gtttatgtaa aactcaactc cattttgtag aaaaaagtta
     8281 atgtggagaa aagttaagag aatgttacga tttagctaga cattcctgtt taaacgccta
     8341 caatttgccg tagtttttat ttttaatttt atttccggag tatttatgga aacaattgta
     8401 attgtaggtg gcggtgcagg cggtttggag cttgcaacct atctaggcaa caaattaggg
     8461 cgtaaacaaa aggcaaaagt ggtgctgatt gaccgcaatg caacgcattt atggaaacca
     8521 ctgttgcacg aggtggcaac cggtactatt gatgagggaa ctgatgcgtt aagctatcgg
     8581 gctcacgcta aaaatcatag ttttgaattt cagcaaggta cgctggtggc ggtagataga
     8641 gagaagaaaa cggtttcact cgccccgatt tataatgatc aaaaccaact tctcgttaaa
     8701 gaacgccata tcagttatga caaattagtg atcgcgatcg gcagtaaatc caatgatttt
     8761 ggcaccaaag gggttgcaga aaattgcatt ttcttagatg gctcagagca agcaaaagat
     8821 ttccaaaaac gaatgatgga gctgttttta cgtttttcac atagtgaaaa taaagatgta
     8881 aaaattgcga ttgtgggggg gggagcaaca ggtattgagc tttcagcgga gctttaccac
     8941 gtggtgaaaa atttaaattc ttacggcttt ggtaaattaa accgtgccag cttgaaagtc
     9001 actttaattg aggcaggccc tcgtttgatt ccggcattac cggagaaagt gtcggtttct
     9061 gctttccacg aattgaaaaa agcgggtgtt tcggtgcgtt taggtacgat gattaccgaa
     9121 gctcgtccag atgggctaat gacccgttta ggtgagaaaa ttgaggcgga tattatggtt
     9181 tgggcggctg gcgtgaaagc ccctgaaatt acccgtgaat ttggttttga aactaaccgc
     9241 ttgaaccaaa ttgagattaa agacacgcta caaaccaccg ttgatgagtc agtgtttgtc
     9301 attggcgatt gtgcatcatt aattcaagat ggcaagccaa ttccacccag agcacaagca
     9361 gcccaccaaa tggcaacccg atgtggtaaa aatattgtgg cattgataga aggaaaagag
     9421 atgaagccgt ttcgatttaa cgataaaggc tcactgcttt ccttctcaaa attcggcacg
     9481 gtgggtaatt taatggggaa tttagtgagc ggtgatatgt ttattgaagg taaaattgcc
     9541 cgcttggctt acctctcgct ttatcgaatg caccaagtgg cattacacgg ctgtgtcaaa
     9601 accggcttga ttgtgttggt agggcaaatt aaccgtttct taagaccaac aatgaaacta
     9661 cattaaacaa aaatccgcat caaatgatgc ggatttttta tggcaaaata acaagcggtt
     9721 attttcttgc ctatttttgc aaatactcac gcctagtttg ctaatttaat ttccgccaac
     9781 catttatcaa ttaagcgttt gccctcttcg ctggaacctg ctttttggta atcaatttcc
     9841 acaaagtcta acgcttcggc tttggttgat tgtggagcaa cttcggcgtt gatgtttgtt
     9901 ggggtttggt aaacaccgtg ttttttccaa ggaatttcct gtgcttcttt tgaaagcacc
     9961 caatccataa agagtttggc gttatctaaa ttacgggcat ttttgataat gctgacaccg
    10021 cctaacgcat agcccacttt accttccgga ataatggctt caataggcgc acctttttct
    10081 ttttcagtgg cataactatg tacaaagcct accgtaaccg cactttcacc acgagcaaga
    10141 ttgctggtga ctagggtgct tttcacatat tgcgaaacgt tattgttgag ttttttcaga
    10201 taatcaaagg ttttctcttc gccccataag gttgaaagag tggtcataaa cgtataagtc
    10261 gtgccggagc tacgaggatc aggcagttgt acttcacctt ttaagcgagg atctgataaa
    10321 tcttcccatt ttttcggtgc ctcaatgcct agtttggcga gtttttcggt attcacgcct
    10381 aaccctaata caagcatata aacgatagag gtaaatttac cttgttcact ttcaactaag
    10441 gatttaaatt gtggcagaat ttcagcttgt tttggtgaac ggtaggcttc cagtaagcct
    10501 aattcgcctg cttgaaagtg aggttcaatc gtgccgccat accaaatatc tgcctgaggg
    10561 ttcgatgctt ccgcttttac ttttccgaaa attgtaccgg ttccgccgtg aatgaattta
    10621 gtttcgacat tatatttgtg tgaaaattgc ttggtcattt cttcacaaac attattttgt
    10681 accgagcaat aaacagttaa acgaccttct gcttgtgctg cttgagctga taagaaaatg
    10741 ccagaggcga ttaagatcga ttttaatgag attaatttga gtacgttcat cactttcctg
    10801 aaaacataaa ataaaaggta gtgaagtcta gcatttcaaa gcgctggttg aaaatagtta
    10861 attgcaatga agtagggcaa ttgtgcctat tactttaata tttaccaact atttgcaagt
    10921 tttagcaaaa aataaccgct tgtagtagct cttg
//
LOCUS       DANLRK010000086        10892 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.086, whole genome shotgun sequence.
ACCESSION   DANLRK010000086 DANLRK010000000
VERSION     DANLRK010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 10892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10892
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.086"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(76..381)
                     /locus_tag="PXA33_001570"
     CDS             complement(76..381)
                     /locus_tag="PXA33_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HDL5120891.1"
                     /translation="MRKPAHPGQILLDGFIEPNNIKIKELADHLGFSRETLSRVLHGK
                     TPMTANLALSLEEAGISTAKLWLNLQTKYDLWELKQQRLNPVMPFHFGGNYGETAHA"
     gene            complement(390..665)
                     /locus_tag="PXA33_001571"
     CDS             complement(390..665)
                     /locus_tag="PXA33_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011039853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL5120892.1"
                     /translation="MIKSFKHKGLKQYFEKGITKGIQLNHQRKIDGILDLIDSAESID
                     EFMPFYQCHELKGNRKGIYSMTVNGNWRITFEFVNGDAYILNYEDYH"
     gene            957..1187
                     /locus_tag="PXA33_001572"
     CDS             957..1187
                     /locus_tag="PXA33_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5120893.1"
                     /translation="MNAQATLNNHKDYILCGRKEKRTSDFINVFEVFENEATQEFVIE
                     RAMFRNGKLIDWNQSDKMNAEQAQQLWQAYIH"
     gene            1569..1805
                     /locus_tag="PXA33_001573"
     CDS             1569..1805
                     /locus_tag="PXA33_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5120894.1"
                     /translation="MSNQEFVKLGSVEPSTIDELQKEDIRQAILKSVSITPYYVGLEI
                     AVCQAISAINKFSDGCITEELLDTKNGDKYRKNN"
     gene            2112..2187
                     /locus_tag="PXA33_001574"
     tRNA            2112..2187
                     /locus_tag="PXA33_001574"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2145..2147,aa:Arg,seq:tct)
     gene            2374..2880
                     /locus_tag="PXA33_001575"
     CDS             2374..2880
                     /locus_tag="PXA33_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5120895.1"
                     /translation="MKTKYKIIIERAKQQGLTHIELTQKHCLYLATSVGKLCYGVANN
                     AEQHIKLAAGEIFTLLTIINHQIQFDEDLELHTWKASGTTPPEQRAIRMLHHTSQFAW
                     FAPWLVDSNEKEQNQNTIRINLFNKCSGILIELNGILKEYELTLDECLDEFLKNKHCL
                     NEFSIHKE"
     gene            2884..3243
                     /locus_tag="PXA33_001576"
     CDS             2884..3243
                     /locus_tag="PXA33_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5120896.1"
                     /translation="MTTFSIYTSVWHDSSTKGFRHNIKHKRHDCWRADIRIKQKINDV
                     WVQISRIRKRFKCPEQAKSFVEQWKQIDSPAFMQHQINRQALREELSNLEQRKQKQDG
                     ILEVIEYRISQIKELLK"
     gene            3240..3719
                     /locus_tag="PXA33_001577"
     CDS             3240..3719
                     /locus_tag="PXA33_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5120897.1"
                     /translation="MKRNKVESYTIRLLKNNHTKRYFGEVILNGIKEISTAEMMSEIN
                     AIKAVNKRIEALNKTKAVNLPNYPEIRGHQPIMYNYNFEQIEEDPEQVKPKPSKPKRK
                     PFIPYGLNGYFVDSNGNIRLHLDRRASAETITLETDFFNVLAEMVKRTQEANNAKHA"
     gene            3853..4065
                     /locus_tag="PXA33_001578"
     CDS             3853..4065
                     /locus_tag="PXA33_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5120898.1"
                     /translation="MIGYEDYTDASLQPEEDPSEEAFILAEENIIELLENNGFETIAT
                     ILAGSDGWRNAINAYLAEQNKHRNEF"
     gene            4078..5028
                     /locus_tag="PXA33_001579"
     CDS             4078..5028
                     /locus_tag="PXA33_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5120899.1"
                     /translation="MTTANQQFELILKTESRVVSTNLQTFEEQANQYLATLTTTFETD
                     DDFAKAKEEVKELESIEKKIRDAIKQTQTGEIAELVLTAEQIAERFRQERLTRDKLVK
                     SKETEIKKQIADKAIEEITDTRNKLVKVSDISLALEITMPKHGIANRIAEAQKNKRTI
                     DSLTKAVNAEKALIISEMSVEIGRLTERLEQLAAKSAYLFPDAVKLIATQDDLAPIIQ
                     QRLAEEAEREAAIKAKAEADKVQAEAKAITDKMERKQAVENPQNVAKMNIEEPAQTDG
                     PLGDFIITIRLNQTIQTNAVSIARELKAKFGDSVSLNKAK"
     gene            5069..5863
                     /gene="bet"
                     /locus_tag="PXA33_001580"
     CDS             5069..5863
                     /gene="bet"
                     /locus_tag="PXA33_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HDL5120900.1"
                     /translation="MTTALQTLTNKLAERFEMGDGSGLVETLKSSAFAGATVSDAQMI
                     ALLVIANQYQLNPWTKEIYAFPGGNGGLTPIVGVDGWVRIINREPQYDGMEFHFTDDY
                     SACTCTIYRKDRSKPIVVTEFMGECKKSSPAWNSHPKRMLRHKAMIQCARLAFGFTGI
                     YDQDEAERIAENEKPPKNITPQNNVVETTAVELISEEQLSQIRQLMQVTGTEEAKILA
                     YIGVQALNQIPKSQAEAVIKKLNLTLDKQNAEKADNGESVGEEIPL"
     gene            5860..6495
                     /locus_tag="PXA33_001581"
     CDS             5860..6495
                     /locus_tag="PXA33_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HDL5120901.1"
                     /translation="MTGDLIILDCEQGSEEWLNARLGIPTATGIENIVTPSGKKSSAW
                     NSYLAELVAESIEGAKDGFKSSDMARGNELEPLARMAYEFETDTEVVQVGGVYLNAEK
                     QLMVSPDGLIPNLKKGLEIKCPKMKTHIKYLLEGGVPNEYLIQVQSALWVTGYETWDF
                     VSYCPEYQKQPLYIYTAERDPVLMKAFDQYIPQFIKSLYALKGVTAPKGAF"
     gene            6508..6684
                     /locus_tag="PXA33_001582"
     CDS             6508..6684
                     /locus_tag="PXA33_001582"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5120902.1"
                     /translation="MTTQKKYELLQNDTINHNGRTLYRIKAICHFRRTAEYSARATHA
                     TAAECYGGIACTST"
     gene            6705..7157
                     /locus_tag="PXA33_001583"
     CDS             6705..7157
                     /locus_tag="PXA33_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HDL5120903.1"
                     /translation="MKPFDLNKALAGEPVKLRNNDKAFVKYLISDDYIRDNKDHQVQG
                     YTVDEENVFLSEVSWAVSGSHFNDGTIAQYDIVGMWEEPRPAVTLTLPCPLKEPQENM
                     WFIDNDFTIVKSMFANAPFVKKFLPQGRCFASEEDAQEWLDAMRNSRR"
     gene            7235..7762
                     /locus_tag="PXA33_001584"
     CDS             7235..7762
                     /locus_tag="PXA33_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HDL5120904.1"
                     /translation="MNEEKYFSVDVSDETHVIRLHETLEQAKQSCLNGATEAYEFAGD
                     MDDHESYESYEAYDLPYAVYGVVLGRAKSDIRPLTDEERASELFGEAEQVIEPPTLLE
                     NNGWISIEDKLPPIETDVLGLCDISGMQLILIVSRELADNEWYFLSVNQYGLDDDVIA
                     VTHWQPLPEPPKEEK"
     gene            7759..7962
                     /locus_tag="PXA33_001585"
     CDS             7759..7962
                     /locus_tag="PXA33_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5120905.1"
                     /translation="MTDTERLDFIAQRKLDVRYSDLVDEFIIASYTKPHEQYFHWARN
                     ADLRTAIDEAMKEMRGLNNATTN"
     gene            7946..8410
                     /locus_tag="PXA33_001586"
     CDS             7946..8410
                     /locus_tag="PXA33_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HDL5120906.1"
                     /translation="MQQLIKNIEQWAEDRNLINGSTPQKQMLKLMEEFGELCGGIARN
                     NPEMIKDAIGDLIVVCIIKAKQEKQPIYPVQFENHRLNGYRFDISVAVHCLYDIERIK
                     DRGKYSRFFGILHHLALINRFTLNDCLAHAYDQIKDRKGEMQAGIWVKEDDL"
     gene            8504..8674
                     /locus_tag="PXA33_001587"
                     /pseudo
     CDS             8504..8674
                     /locus_tag="PXA33_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253375.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8910..9401
                     /locus_tag="PXA33_001588"
     CDS             8910..9401
                     /locus_tag="PXA33_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL5120907.1"
                     /translation="MQTKPILDACCGSRMFHFDKQNPLVLFADNRQIETTMKDRDKIR
                     HLEIKPDVLHDFTNMPYENESFWHIVFDPPHLVQGGENSWLVKKYGKLPCNWREVLKK
                     GFDECMRVLKPNGTLIFKWNETQIPVSEILKVIDCQPIYGHKSGRLNQTHWLAFVKIE
                     QSL"
     gene            9428..9685
                     /locus_tag="PXA33_001589"
     CDS             9428..9685
                     /locus_tag="PXA33_001589"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5120908.1"
                     /translation="MEQIKLSEKAERALILSQLNENSAMIIDLDDVALILNCGYDYVQ
                     SKISKKPGFPEPVQGINGKSVLFKSGDVIRWVNRQARTRKN"
     gene            complement(9682..10671)
                     /locus_tag="PXA33_001590"
     CDS             complement(9682..10671)
                     /locus_tag="PXA33_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL5120909.1"
                     /translation="MASIRKRGSKYQVEISKKGVRKSATFRTKAEAQAWAYEEERKIE
                     IISKGGVVDILFAEALERYQNEVSINKRTWRREIGRINNILKEPIANRYVQDIKRDDI
                     EDWIEARLKRGLKGSSVERDLSILSNVFTYCLKRWGYISENPMIGVIHPNKVPPRKQR
                     YSQEDIDKILEVSKYREDIKRSNARLAAAMLFAIETAMRAGEISGLTWDNVNFEKRIA
                     HLPMTKNGSSRDVPLSSKAIEILKRLETIKKDNSVFQLPQGNGLSARFFELKEKAGLS
                     YLNFHDTRREALSRLAKKVDVMTLAKISGHKDVKILLNTYYAPNMEEVAHLLD"
BASE COUNT         3692 a         2218 c         2146 g         2836 t
ORIGIN      
        1 atattccttg aataaaccgc cctcgtggcg gttttctttt cctaagaaca ataaaatcct
       61 ccgaagaggg cattattaag cgtgagcagt ttctccataa tttccaccaa aatgaaaagg
      121 cataacagga tttaaccgct gctgctttaa ctcccataaa tcatatttag tttgtaagtt
      181 tagccataac ttagccgtac taattcctgc ctcttctaaa cttaacgcta aatttgcagt
      241 cattggtgtt ttgccgtgta atactcttga taaagtttca cgagaaaacc caagatgatc
      301 tgctaattct ttaattttga tgttatttgg ttcaataaat ccatctaata aaatttgccc
      361 cggatgtgct ggtttacgca taactccccc taatgataat cttcataatt caaaatataa
      421 gcatcgccat ttacaaattc aaatgtaatc cgccagttgc cattaaccgt catagaataa
      481 atgccttttc gatttccttt taactcgtgg cattggtaaa aaggcataaa ttcatcaata
      541 ctttctgctg aatcaatcaa atctaaaata ccatcgattt tacgttgatg attaagctga
      601 ataccttttg ttattccttt ttcaaaatat tgttttaagc ctttatgctt gaagctctta
      661 atcataataa cacctagcaa acacaatgtg atacaaatat atcacgcaca ataaaaaaga
      721 caatcttttt taagtgcttt ttctcctaat tgtctttctc ttcttatgtt aagtgtttaa
      781 aaaacaagca atcaaacaac ctttctaaaa ataatttctc tttaaaatca attatttaat
      841 cacgaattga aatttgtttg gctaaaataa ttacaaattg aattgactta atgatttcaa
      901 cttgtgataa taaacccatc aaaacaaaca acgccacaga caaagaggaa accaaaatga
      961 acgcacaagc aacgctaaat aaccacaaag actacatttt atgcggacgc aaagaaaaac
     1021 gcaccagtga tttcatcaat gtgtttgagg tctttgaaaa tgaagccacg caagagtttg
     1081 tgattgaaag agcaatgttc agaaatggca agttaatcga ctggaaccaa agcgacaaaa
     1141 tgaacgccga gcaagctcaa caactttggc aagcctacat tcactaagaa tttttatcaa
     1201 agcccttcac ggagggcttg aataaaggtt ctaaacctcg ccgaaaggca ttgttcttta
     1261 aaaattaaac caaaaacaca tcacgacaag cggattactt agcaatgtaa tgacattgga
     1321 gagacagaac caatctttgc ataaagcggt aaaacgtatg cggtaaatga acttataggc
     1381 aagttgtgaa actctctaag ctactcggac tgaaagaata gtacggtagt tgtggcgaat
     1441 ttactggtga tgtgcgaact agcggaaagg ttaagcactg gcagtgaatc gggaagcaca
     1501 aacgctggtt caattccaaa gcatatttga gaaagtgtgc tttggaatat taataacagg
     1561 agaacaaaat gagtaatcag gaatttgtta aattaggcag cgttgaacct tcaacgattg
     1621 atgaattaca gaaagaagat attaggcagg caatattaaa atccgtctct ataacgccat
     1681 attatgtggg gctggaaata gcggtttgcc aagcaatatc agccatcaat aaatttagtg
     1741 atggctgtat tacagaagag cttttagata cgaaaaatgg tgataaatac cgaaaaaata
     1801 actaatcaat aacaccaaag tgatttcatt ttttcggcat tagccctaga tgttgcccaa
     1861 gtattatatt cctcgaagtt gtggcaaaca agaggacttg gcccggtggc tcacaagtat
     1921 aaaaaagagc caccatctct aacagctctc aacagagagt gagacaaata accccgtttt
     1981 agttttaatg ttttctaaag tcggcgttag gcagcgaaac gaatgtacta gagagttgtt
     2041 agagatggtt agtccatcac attccttaga ttgaagtaag ccctgccgtt ggggttaagc
     2101 aacggtactt tgaccgcata gttcagtgga tagagcagct gccttctaag cagtgggtcg
     2161 agagttcgaa tctctctgcg gtcgccatcc aaaaccgctt tacgcacatc atcgatgacg
     2221 agcctacgaa gtggaaacct accgtgtaga gcggttttga atggctaagt tgtgtaactg
     2281 ctacaactta aaaagagcgg ttgataataa acataatgac tccgtttccc cgcctcgtgc
     2341 ggggcttttt ttatccacat aaaaggattc aaaatgaaaa ctaaatacaa aattattatt
     2401 gagcgagcaa aacaacaggg tttgacccat attgagctta ctcaaaaaca ttgtctgtat
     2461 ctcgctacat cagtagggaa actgtgctac ggggtagcta ataatgcaga acaacatatt
     2521 aaacttgctg ctggagagat ttttacacta ctaacgatca ttaaccatca aatccagttc
     2581 gatgaagact tagagcttca cacgtggaaa gctagtggaa caaccccacc cgagcaaaga
     2641 gcgatacgta tgttacatca tacctcgcaa tttgcttggt ttgctccttg gttagtggac
     2701 agcaatgaaa aagaacaaaa ccaaaataca atcaggatta atttatttaa caaatgctct
     2761 gggattctga ttgaactaaa tggcattctt aaagaatacg agctaactct tgatgagtgc
     2821 ttagatgaat tcttgaaaaa taagcattgt ttgaatgaat ttagtattca caaggagtaa
     2881 aaaatgacga cgtttagtat ttataccagt gtatggcacg acagctccac gaaaggcttt
     2941 cgccacaaca ttaagcataa acgccacgat tgctggagag cggatatccg cataaagcaa
     3001 aaaatcaatg atgtgtgggt gcagatttca aggatacgca aacgcttcaa gtgccctgaa
     3061 caggcaaaat cctttgtaga acaatggaaa caaattgatt cacctgcatt tatgcaacac
     3121 caaatcaacc gccaagcatt aagagaagaa ttatcaaacc ttgagcaacg caaacagaag
     3181 caagacggaa tacttgaggt gattgagtat cgtattagcc aaatcaagga gttgctgaaa
     3241 tgaaacgcaa taaagtagaa agctatacta tccgcttatt aaaaaataat catacaaaac
     3301 gctacttcgg agaagttata ttaaacggca taaaagaaat cagcacggca gagatgatgt
     3361 ctgaaattaa cgctattaag gcagtaaata agcgcattga agctctgaat aaaaccaaag
     3421 ccgttaattt gcctaattat cctgaaatcc ggggtcatca gcctattatg tacaattaca
     3481 attttgaaca aattgaggaa gatcctgaac aggttaagcc taaaccatca aaaccaaaac
     3541 gcaagccatt cataccttat gggctaaacg gctactttgt ggatagcaac ggtaatattc
     3601 gcttacatct agacagacga gcaagtgccg aaacaattac ccttgaaact gatttcttca
     3661 acgttcttgc cgaaatggta aaacgtacac aagaggcaaa caatgctaaa catgcttaga
     3721 caatggatta aatacggctt atacgccctg ctttttatgg ttgccatcac cctcctaatg
     3781 caatgaagag cttatccctg aaagcacctg caagcctgaa acttgcgaat ttaaagtaac
     3841 ggagaaagtg tgatgatagg atacgaagac tacacagatg cttcacttca accagaagaa
     3901 gaccctagcg aagaggcttt catcttggct gaagaaaaca tcatcgagct attagaaaac
     3961 aacggctttg aaaccattgc caccatccta gccggttctg atggctggcg taacgcaatc
     4021 aacgcctacc tagccgaaca gaacaaacac agaaatgaat tttagaggaa taagaaaatg
     4081 acaactgcaa atcaacaatt tgaacttatt ttaaaaaccg aaagccgagt ggtttctacc
     4141 aacttgcaaa cttttgaaga gcaagcaaat caataccttg ccacgctcac caccaccttt
     4201 gaaaccgatg atgattttgc aaaagcaaaa gaagaagtga aggagcttga aagcattgag
     4261 aaaaaaattc gtgacgcaat taaacaaacg caaactggcg agattgcgga attggtatta
     4321 acagcagaac aaatcgctga acgtttccgc caagagcgat taacccgaga caagctcgtt
     4381 aagagcaaag aaacggaaat caaaaaacag attgccgata aagccattga agaaatcact
     4441 gataccagaa acaagctggt taaagtaagt gatatttccc ttgccttaga aatcacaatg
     4501 ccaaaacacg gcattgctaa tcgtattgcc gaggcacaga aaaataaacg cacgattgac
     4561 agcttaacca aagccgtgaa cgccgaaaaa gcgctgatta tcagcgaaat gtcggtagaa
     4621 atcgggcgct tgactgagcg attagaacag cttgctgcaa aatcagctta tctttttcct
     4681 gatgcagtga aattgatcgc aacccaagac gacctcgccc caattattca acaacgcctt
     4741 gccgaagaag ccgagcgtga ggcggcgatt aaagccaaag cagaagccga caaagtacaa
     4801 gccgaagcca aagcgattac tgataaaatg gagcgcaaac aagcggtcga aaatccgcaa
     4861 aatgtggcaa aaatgaacat cgaagaaccg gcacaaaccg atgggccatt aggcgatttt
     4921 atcattacta ttcgcttaaa ccaaacaatc caaaccaacg cagtcagtat tgctcgtgag
     4981 ctgaaagcga aatttgggga tagcgtttcg ctcaataagg ctaaatgatt tgataccgca
     5041 caaattcaac ctaaataagg atcatattat gacaactgca ttacaaacac taaccaacaa
     5101 acttgccgag cgttttgaaa tgggagatgg ctcaggatta gttgagacac ttaaatcgtc
     5161 tgctttcgca ggggcaaccg taagcgatgc tcaaatgatt gcactattgg ttattgctaa
     5221 ccaataccaa ttaaaccctt ggacaaaaga aatttatgca ttccccggtg ggaacggtgg
     5281 attaacgcct attgtcggcg ttgatggttg ggttcgcatt attaaccgtg agcctcaata
     5341 tgatggaatg gaatttcatt ttaccgatga ctattccgcc tgtacttgta ctatctaccg
     5401 taaagataga agtaaaccga ttgtggtgac ggaatttatg ggggaatgta aaaagagctc
     5461 tccagcttgg aactctcacc caaaaagaat gttacgccat aaagcaatga tccaatgtgc
     5521 aagattagcc tttggcttta cgggtattta cgaccaagac gaagcagagc gtattgcgga
     5581 aaatgaaaaa ccacctaaaa acatcacacc acaaaataac gtggtagaaa ccactgccgt
     5641 tgaactcatt tcggaagaac aactgtcgca aatccggcag ttaatgcaag taaccggtac
     5701 ggaggaggcg aaaattctcg cctatattgg cgttcaagca ctgaaccaaa tccctaaatc
     5761 gcaggcagag gcagtgatta aaaagctcaa tcttacgcta gacaagcaaa acgctgaaaa
     5821 ggcggataat ggcgaaagtg tcggtgagga aatcccacta tgacaggcga tttaatcatt
     5881 ttagattgcg aacaaggatc agaagaatgg ctcaatgcca gattgggcat tcccaccgcc
     5941 acaggcattg aaaatatcgt tacgccaagc ggtaaaaaat cgtccgcttg gaatagctat
     6001 cttgccgaac tggttgccga aagcattgag ggagcaaaag acggttttaa gtcgtctgat
     6061 atggcacgag gcaacgagct tgaaccgctt gcacgaatgg cttacgagtt tgaaaccgac
     6121 accgaagtcg tgcaagtcgg cggtgtgtac cttaacgccg aaaaacagtt aatggtaagt
     6181 cctgacggct taattcccaa tctaaaaaaa gggttggaaa tcaaatgccc gaaaatgaaa
     6241 acccacatca aatacctgct agaaggcggt gtgccgaatg aatatcttat tcaagtgcaa
     6301 tctgcccttt gggtaacagg ctacgaaacg tgggactttg tcagctactg ccccgaatac
     6361 caaaaacaac cgctttacat ctacacagcc gaacgtgatc cggtattgat gaaagccttt
     6421 gatcaatata tcccgcaatt tatcaaatcc ctttatgcat tgaagggggt aaccgccccg
     6481 aaaggggctt tttaataggt agccaatatg acaacccaaa agaaatacga actactccaa
     6541 aacgacacaa tcaaccacaa cggcagaaca ctctaccgca tcaaagccat atgccacttt
     6601 aggcggacgg ctgaatattc cgcaagagcc actcacgcca cagccgctga atgctatggt
     6661 ggaattgcct gtacatcaac ataaccacaa acacggagaa taaaatgaaa ccatttgatt
     6721 taaataaagc attggcaggt gagccagtaa aactaagaaa taatgataag gcatttgtaa
     6781 aatacctaat tagtgatgac tatattagag ataataaaga ccaccaagta caagggtata
     6841 cagttgatga agaaaatgta tttttgtcag aagtatcgtg ggctgttagc ggatctcatt
     6901 tcaatgatgg aacaatagct caatatgata ttgttggtat gtgggaagaa ccaagaccag
     6961 cagtaacatt gacgttacct tgcccattaa aagaacctca agaaaatatg tggtttattg
     7021 acaatgattt taccatagta aaatctatgt tcgcaaatgc cccctttgta aaaaaattct
     7081 tgccacaagg gcgatgtttc gcttcagaag aagacgctca agaatggtta gatgcaatgc
     7141 gaaatagtcg taggtaaatc ggcagcacta acagctgaat gctgctgttg aatttcttgt
     7201 atatccacat aaacacggaa cgcggagaaa gaaaatgaat gaagaaaaat atttttcggt
     7261 cgatgtctcg gatgagacac atgtaataag gttgcacgaa acattagagc aagcaaaaca
     7321 aagctgttta aatggtgcta ctgaagccta tgagtttgcg ggtgacatgg atgatcacga
     7381 aagctatgaa agctatgaag cctatgattt accctatgca gtttatggtg ttgttttagg
     7441 tagagcaaaa tcagatattc gtccactaac agacgaagaa agagcatcgg agttatttgg
     7501 ggaagctgaa caagttattg aaccaccaac acttttagaa aacaacggct ggatttccat
     7561 tgaggacaaa ttaccaccta ttgaaacgga tgtgctaggg ttatgcgata tttcaggaat
     7621 gcagttaatt ttaatagttt caagagaact tgccgataac gagtggtatt ttcttagtgt
     7681 aaatcaatat gggcttgatg acgatgtgat tgcagtaacc cactggcaac cacttcccga
     7741 accaccaaag gaggaaaaat gaccgacacc gaacgcttag actttatcgc ccaacgcaaa
     7801 ttagatgtcc gatattctga tttggtggac gaatttatta ttgctagcta taccaaaccg
     7861 cacgagcagt attttcattg ggcaagaaat gccgatttac gcacggcgat tgatgaagca
     7921 atgaaagaaa tgagaggact aaacaatgca acaactaatt aaaaacatcg aacaatgggc
     7981 ggaagaccgc aatttaatta acggctcaac cccacaaaag caaatgctca aactgatgga
     8041 agagtttggc gaactctgcg gaggcattgc ccgcaataat cctgaaatga ttaaagatgc
     8101 gattggggat ttgattgttg tctgtattat taaagcaaaa caagaaaaac aacctattta
     8161 ccctgtacaa tttgaaaatc atagattaaa tggttacaga tttgatattt cggtcgctgt
     8221 tcattgtcta tatgatattg aacgcattaa agacagaggt aaatatagcc gtttttttgg
     8281 catattacac catttagcct taataaatag atttacgctt aatgactgcc tcgctcacgc
     8341 ctacgaccaa atcaaagatc gcaagggcga aatgcaagct gggatttggg ttaaagaaga
     8401 cgatttatag tacctaagct ataaaaaccg aaacatcttg cagaaaagca ttccgccact
     8461 ctataagagt ttcggtttct tcttcgttgg cgttgattat gtttacacgc acgctaccga
     8521 gtatcaaacc acgcttggcg taatgaagcg gatacttgaa ccgatgttag ccgacttcga
     8581 ggtcgatccg tttgaagagg ctcactacca caaagcccta actaccttaa ggcaatacca
     8641 gccgaaaggc ttaggcggct tagtccgaat ttaatttgca aaaaaatcac acaatccgac
     8701 cgcttgtgaa acaacaagcg gcggatttcc atacccaaat tttgaggaca agccgatgaa
     8761 atacgcaaaa atcattctat ttttaaccgc ctttgcagtt gctgctgacc atctagagtt
     8821 acgcaacgac tgcgatggca aaatttgtat ggctcaacgc taattaaccc aaccgcttgt
     8881 tccgcaagcg gttatttttt ggaggaaaaa tgcaaacaaa accgatatta gatgcgtgtt
     8941 gtggttcccg tatgtttcat tttgacaagc aaaatccact cgtcctgttt gctgataatc
     9001 gacaaataga aaccacaatg aaagaccgag acaaaatccg ccatttagaa atcaaacctg
     9061 atgttttaca cgatttcacg aatatgccat acgaaaacga gagtttttgg cacattgttt
     9121 ttgatccgcc acatttagtt caaggtggag aaaatagctg gcttgtaaag aaatacggca
     9181 aattaccttg caactggcga gaagtcttga agaaaggctt tgacgaatgt atgcgagtgc
     9241 taaaaccaaa cggcacgctt attttcaagt ggaatgaaac acaaatcccc gtttcagaaa
     9301 ttttaaaagt tattgattgt cagccgattt atggacacaa aagcggacgc ttaaaccaaa
     9361 cacattggct ggcgtttgtg aaaattgagc aatccctcta aatgaggggt ttatttttgg
     9421 agagaatatg gaacaaatta aactttcaga aaaagcggag agagccttaa ttctttctca
     9481 gctcaacgaa aattcagcaa tgattattga tttagatgat gttgccctga ttttgaattg
     9541 cggatatgac tatgtgcaat ccaagatcag taaaaagccc ggtttcccag agccagtgca
     9601 aggcattaac gggaaatcag ttcttttcaa aagtggcgat gttatccgtt gggtcaaccg
     9661 tcaagcacga acacgcaaaa attaatccag caaatgagcg acttcttcca tatttggggc
     9721 gtagtaagtg ttaagcaaaa tcttcacatc tttatgccct gaaattttgg ctaaggtcat
     9781 cacatcgact ttttttgcca agcgtgaaag agcctctctc ctcgtgtcgt gaaaattcaa
     9841 ataagaaagc ccagcctttt cttttaactc aaagaatcta gcacttaaac cattcccttg
     9901 tggaagctga aaaacggaat tatccttttt gatggtttcc agtcttttca agatctcgat
     9961 tgcctttgat gataacggaa cgtctcttga tgaaccgttt tttgtcatag gcaaatgagc
    10021 tattcttttc tcaaaattca cattatccca cgttaaaccc gaaatctcac cggcacgcat
    10081 tgccgtttca atcgcaaata acatagccgc agccaatcga gcattagagc gtttaatatc
    10141 ttctcggtat ttgctgactt ctaagatttt atcaatatct tcctgggaat aacgctgttt
    10201 tcttggtggc actttgtttg ggtgaataac accgatcatc ggattttcac taatatagcc
    10261 ccagcgtttt aagcagtagg taaatacatt gcttaaaata gacaaatccc tctcaacact
    10321 tgagcctttt aaccctctct tcaatctagc ttcgatccaa tcttcaatat catctcgctt
    10381 aatatcttgt acataacgat tcgcaatagg ctctttcaat atgttattga ttcgacctat
    10441 ctctcgtcgc cacgtcctct tattaatcga tacttcattt tgataccgct ctaacgcttc
    10501 cgcaaataaa atatcaacta ccccaccttt cgagatgatc tctattttac gctcttcttc
    10561 gtaagcccat gcctgtgcct ctgcttttgt gcggaaggta gctgattttc tcaccccttt
    10621 cttcgagatt tcgacttgat atttactgcc ccgcttgcga atgctcgcca tatttttacc
    10681 tgccttttgt cgtaaatttg tcgtaattta taccatacaa taaccgacaa taacagataa
    10741 taaccgattt tatgaaatca tcttaaggca aagaagagag gaaaaactta agataaccta
    10801 ttgaaataac tataataaac gataataacc gacaaaaaac ggaaataaaa aaaagcccct
    10861 aatcaaaaaa gcgaaaaggg gcggaagtct ta
//
LOCUS       DANLRK010000087        10680 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.087, whole genome shotgun sequence.
ACCESSION   DANLRK010000087 DANLRK010000000
VERSION     DANLRK010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 10680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10680
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.087"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            38..1225
                     /locus_tag="PXA33_001591"
     CDS             38..1225
                     /locus_tag="PXA33_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5120910.1"
                     /translation="MSALINLFYLYDPWFFHFLRMSFVVGLVACGYLVFQIIKGKSNG
                     IIIPKDSLIAIIALIGFSIIPLLINSTKEIGVLAMYVKMLILFVFGIAIYNVFYHNQQ
                     GKTLFVRDLKIGIAIQWICGVTALLGLPFMVDFLLSSNAMMPRFFGSEQEYRLYNITS
                     SAFFQLSIFYLMLLHFLLAYNKKTDVVNEWFLFLLLCVGLISGRTFLLMSVISIALYF
                     KWKYILPLIAFTALILFLAIVFPENRYVEHALEPVINLLSGTGKVSSSTDNLVKNHLF
                     LPTLNQFISGDGLYYTETGGYYGGSDSGYIRQVLYGGVSYMLACFAFTYYFVRKVAVN
                     WFEGSWIFTLSTIGILAILNIKADTFAFPGLMMLFLMFLSLFGTSGRNVTFFCKNKEA
                     EYV"
     gene            1218..2183
                     /locus_tag="PXA33_001592"
     CDS             1218..2183
                     /locus_tag="PXA33_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005578999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL5120911.1"
                     /translation="MFSIIVPSYNRNQEINALLESLKQQTAYNFEVIIVDDCSKIPVS
                     ETLGILNEFNDISTKEKVRELLTRDSLSHIYSYPFDVKVVRNNPNVGAAQSRNVGATN
                     ATQEWLLFLDDDDRFMPEKCERLAAEIANNPTANFVYHPAKCEMVNEGFTYVTKPFKH
                     KSELTKENILKANKIGGMPMIGLKKSLFCKIGGLSDRLRSLEDYDFLLKLLEEPSFEP
                     LYVDEALTYCTFHTKRSSVSTDTTNTEKAIEYIQQTYIKTPEANNFKLNSLYILSYPH
                     VMNLSRKAAGYYFEMFKQTKNIKQLIIALVIFISPKLAINLKRFM"
     gene            2183..2983
                     /locus_tag="PXA33_001593"
     CDS             2183..2983
                     /locus_tag="PXA33_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_162680708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL5120912.1"
                     /translation="MKFSVLMSLYIKEQPQYLRECFESLKVQTVQADEIVVCFDGAVT
                     PELEAIVEEYSAILPINAVKFPQNRGLGKTLNDGLTYCKNEWVFRMDTDDICMPERFE
                     KQVAFIEQNPETVIFGGQIAEFGQDIHDLVAYRNVPTTVEEIVRFTRLRCPFNHMTVA
                     YQKSKVLEVGGYQDLQEDYYLWIKLIAQFKQVANLPDLLVYARVGNGMVGRRRGMAQA
                     EAEWRLYKLKNRCQIHDPISGFGIFIMRAIPRLLPVSALKKVYGLLRK"
     gene            complement(3020..3526)
                     /gene="msrA"
                     /locus_tag="PXA33_001594"
     CDS             complement(3020..3526)
                     /gene="msrA"
                     /locus_tag="PXA33_001594"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDL5120913.1"
                     /translation="MQNIREIYLAGGCFWGVESFMQRIKGVIKAQSGYANGNTDNPSY
                     QEVCAGSGHAEVVKVTYDANQISLAKLLDYFFKVIDPVSINKQGGDIGIQYRTGIYYT
                     DPQDEPVVTAALAQLQTQFDQPLAVENQMLEQYFPAEEYHQEYLVKNPNGYCHIDIQL
                     MNEILKNQ"
     gene            3707..4969
                     /locus_tag="PXA33_001595"
     CDS             3707..4969
                     /locus_tag="PXA33_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDL5120914.1"
                     /translation="MGTLNSFLGIIVLLFIAFLFSSNKRAINVRTVLGALALQVAIGA
                     LVLYVPAGRDALNAMATGVSKVISYGNEGISFLFGGLVSDKMFEVFGGGGFVFAFRVL
                     PVIIFFSALISLLYYIGVMQWVIRILGGLLQKALGTSKAESMSAAANIFVGQTEAPLV
                     VKPYISKMTESELFAIMAGGLASIAGSVMAGYAGMGVPLTYLIAASFMAAPAGLLFAK
                     IMVPQTEKFRDELEAVDLEKPANILDAAASGASSGMQLALNVGAMLIAFVALIALVNG
                     ILGGIGGWFGYDTLNLGQIFGWVFKPLAWVIGVPWEEAEIAGQMIGLKLAVNEFVGYL
                     EFAKYLAPESTVHLTDKTVAIITFALCGFANFSSIAILIGGIGAMAPSRRGDIARLGI
                     KAVIAGSLANLMSATIAGLFIGLGGAVL"
     gene            5114..5890
                     /gene="deoC"
                     /locus_tag="PXA33_001596"
     CDS             5114..5890
                     /gene="deoC"
                     /locus_tag="PXA33_001596"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HDL5120915.1"
                     /translation="MNLKKSAEIALSLMDLTTLNDNDTDEKVIALCQQANTKFGTPAA
                     ICIYPRFIPIARKALKQQGIEQVKIATVTNFPHGNDDIEIAVAETKAAVAYGADEVDV
                     VFPYKALIAGNEQIGFDLVKQCKAVCAANNVLLKVIIETGELKSAELIRKASEISIQA
                     GADFIKTSTGKVAVNATLESARIMLETIRDLGVADRVGFKAAGGVKTSEEAAQYLALA
                     ESILGNDWVSASHFRFGASSLLANLLATLNDEQQQAVQGY"
     gene            5911..6630
                     /gene="deoD"
                     /locus_tag="PXA33_001597"
     CDS             5911..6630
                     /gene="deoD"
                     /locus_tag="PXA33_001597"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDL5120916.1"
                     /translation="MATPHINAPEGAFADVVIMPGDPLRAKYIAETFLEDAKEVTNVR
                     NMLGYTGTYKGRRISVMGHGMGIPSCSIYAKELITEYGVKKIIRVGSCGAVRNDVKVR
                     DIVIGVGACTDSKVNRIRFRDNDFAAIADFDMTLAAHQAAKAKGIEARFGNLFSADLF
                     YSPDVEMFDVMEKYGILGVEMEAAGIYAVAAEYGAKALAICTVSDHIRTGEQTTSEER
                     QLTFNGMIEIALESVLIGDKQ"
     gene            complement(6706..7557)
                     /locus_tag="PXA33_001598"
     CDS             complement(6706..7557)
                     /locus_tag="PXA33_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDL5120917.1"
                     /translation="MQTHRAIIESIFALAPKNELWATPISGLTVHAADRSIRFNGYIQ
                     DPSLCVVLLGERQICTNGHCQVFGNQEMMFCPVNLPIVTQTLSVAENQPYLAISLRLD
                     FDLIAKIITQLPPQKISENINQRTKWELENEIKDCLLRLLNLLNSPNHIHFLAPLILN
                     ELYFYLLQSEQGGYIRSLLSQDGNIAKIANATKILERKFADTLNMSALAEEVGMSVAG
                     FYAHFKSVTSMTPLQYQKSLRLNAAREKLQAGVSVSETAYQVGYESPSQFSREYKRQF
                     GESPKGR"
     gene            7672..8103
                     /locus_tag="PXA33_001599"
     CDS             7672..8103
                     /locus_tag="PXA33_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HDL5120918.1"
                     /translation="MKIFYHTSATATGGRDGRTQVDDGSIGFDLVGFQNESGKVGTNP
                     EQLFAMGYAACFDSAMNHVAPTLGIKPTKSSTTVAVGIGQKADGAFGLDLDITITVEG
                     ISAEEAKTLISKAHEVCPYSNATRGNVDVRLHVKVVSEFDL"
     gene            8120..8800
                     /locus_tag="PXA33_001600"
     CDS             8120..8800
                     /locus_tag="PXA33_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HDL5120919.1"
                     /translation="MKMLDFETTVKTRQSIRKFLPLPMTETEIKQILADAQNAPSACN
                     TQPWLVHVASGETLARLKARFKKTFEAGINDADFEFNQTKFTGDYEPRWRNQYSHVFT
                     TSYGIPREDKAGRQVVYDDNIVGYGAPHMAFLFMPSIDGHDVNIASDVGMYSQTFLLS
                     LTARGFGGIPQLALAMFAKDVREELGIPEHYKLLHAISFGHPDWDAMQNKHHLGRVPV
                     EESATLHW"
     gene            8864..9988
                     /locus_tag="PXA33_001601"
     CDS             8864..9988
                     /locus_tag="PXA33_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HDL5120920.1"
                     /translation="MINLFTPYTLNNDVEIKNRLVVAPMTHSASNDDGTISEQERHFL
                     HNRAADMGLFITAATGVMRNGKAFYGQPEALDERYLASLTETANILKNQGTKAILQIF
                     HGGAQAIPALLDGANAVAPSEHANGAKALTADEVKQMIQAFANATDLAIQAGFDGVEI
                     HGANGYLIQQFFSTEFNQRTDEWGGSLEKRLRFPLAVVEAAVSVREKHQRPDFIIGYR
                     FSPEEPGEHGLTMTDTYALIDALCEKPLQYLHISLWDFGKKARRGADTNFTRMQLVHQ
                     HINGRLPLIGVGNLLTGEQIRKAYATGWAEFIALGKAVMLNPNVATLLKDNRDSEIVS
                     ELDPQQADHYGIPDRLWGYCVQGGAWLPPLKGQNGQPMDI"
     gene            10072..>10680
                     /locus_tag="PXA33_001602"
     CDS             10072..>10680
                     /locus_tag="PXA33_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HDL5120921.1"
                     /translation="MITLHYLNSSCSHRIVWLLEALGLDYELKIYHREESGFAPMEVK
                     ALHPLGKAPVLQDGDLVLAEGNAIIQHLIARYAEGKFEPTRGSEAYSMYHYWLSISAS
                     LFSANLVTMLANRSDLGAFGDYAKAQTPLYFNHIEQTLSDKKWIVGDELTGADFSLCF
                     PLQWGRNFVEMANYPNIARYLEQVENHPAYQKANQKALKLDMN"
BASE COUNT         3048 a         2027 c         2336 g         3269 t
ORIGIN      
        1 cgtaatttta ctcagcgaaa ttcataatag gtctgaaatg tcagcactca ttaacttatt
       61 ttatctttac gatccgtggt ttttccattt tttaagaatg tcgtttgtag tcgggcttgt
      121 tgcttgtggc tatttggttt tccaaataat caagggcaaa tcgaatggca ttattattcc
      181 taaagatagc ctgattgcaa ttattgcatt aatcggcttt agcattattc cattattgat
      241 taacagtaca aaagaaattg gtgtgctggc aatgtatgtc aaaatgctga ttttgtttgt
      301 gttcgggatt gcgatttata atgtttttta tcataaccaa caaggtaaaa ctttattcgt
      361 tcgtgattta aaaatcggca ttgctattca atggatttgt ggtgtgacag ccttattagg
      421 tttgccgttt atggtggatt ttttattatc cagcaatgca atgatgccac gtttttttgg
      481 ttcggaacaa gaataccgtt tatataacat cacttcttcc gcctttttcc aattaagcat
      541 tttttattta atgctattac attttttatt ggcatataat aaaaaaacag atgtggtgaa
      601 tgagtggttt ttatttttat tattatgtgt tggtttaatt tccggtagaa cctttttatt
      661 aatgtcagta ataagtattg cactctattt taaatggaaa tatatattgc cactgattgc
      721 cttcactgcg ttaattctat ttttggcgat tgtttttccg gaaaaccgtt atgttgaaca
      781 cgccttagaa ccggtaatta atttattgtc cggcaccgga aaagtcagct cttcaaccga
      841 taatttagtg aaaaaccatc tgtttttacc gactttaaat cagtttattt ccggtgatgg
      901 tttatattac accgaaactg gtggctatta tggtggatcg gattccggct atattcgcca
      961 agtattatat ggtggagtaa gttatatgtt ggcgtgtttt gccttcactt actattttgt
     1021 acgcaaagtg gcggtgaatt ggtttgaagg cagttggatt tttaccttat ccaccatcgg
     1081 cattttagct attttaaata ttaaagcaga tacctttgct ttcccggggc tgatgatgct
     1141 gtttctaatg ttcttgtcgc tgtttggaac aagcggtcga aatgtaacat ttttttgcaa
     1201 aaacaaagag gctgaatatg tttagtatta ttgtgccttc ttataaccgt aatcaagaaa
     1261 tcaatgcgtt gcttgagagt ttaaagcaac aaactgcata taattttgaa gtgatcattg
     1321 ttgatgactg ctcaaaaata ccggtcagtg aaactttagg aattttaaat gaatttaacg
     1381 atatttctac taaagaaaaa gtaagagaat tacttactag agatagttta tctcatattt
     1441 atagctatcc atttgatgtg aaagtggtgc gaaataaccc aaatgttggg gcggcacaaa
     1501 gccgaaatgt aggggcaact aatgccacac aagaatggct actgttttta gatgatgatg
     1561 accgctttat gcctgaaaaa tgcgaacgtt tagcggcaga gattgcgaac aatccaactg
     1621 ccaattttgt gtaccacccg gcaaaatgtg aaatggtaaa tgagggtttt acttatgtca
     1681 ccaagccgtt taaacataaa agtgagctaa ctaaagaaaa cattctaaaa gcgaataaaa
     1741 ttggcggaat gccgatgatt ggcttgaaaa aatcactctt ttgtaaaatt ggtggattat
     1801 ctgaccgctt gcgatcactt gaagattatg atttcttact gaaattattg gaagaaccga
     1861 gttttgagcc attgtatgtg gacgaagcct tgacctattg tactttccat accaaacgct
     1921 ccagtgtttc aaccgatacc actaatacag aaaaggctat tgagtatatt caacaaacgt
     1981 atatcaaaac acctgaggcg aataatttta agctcaattc gctttatatt ttaagttatc
     2041 cacacgtaat gaatttatcc cgcaaggcag ccggttatta ttttgagatg tttaaacaga
     2101 ctaaaaatat caaacaactg attattgcgt tggttatttt tatttcgccg aagttggcga
     2161 ttaatttgaa aaggtttatg taatgaaatt ttccgtttta atgtcgttat acattaaaga
     2221 gcaaccgcaa tatttaagag aatgttttga aagcttgaaa gtacaaactg tgcaggctga
     2281 tgaaatcgtt gtttgttttg atggtgcagt tacgcctgaa ttagaggcga ttgtagaaga
     2341 atatagtgca attttgccga ttaatgcggt gaaattccca caaaatcgag gtttaggtaa
     2401 aacgctaaat gatgggctaa cctattgtaa gaatgaatgg gttttcagaa tggatacaga
     2461 cgatatttgt atgccggaac gtttcgagaa acaagtggca tttattgaac agaatcctga
     2521 aaccgtgatt tttggcggcc aaattgctga atttgggcaa gatattcacg atcttgtcgc
     2581 ttaccgcaat gtaccaacca cggtggagga aatcgtcaga ttcactcgct tgcgttgccc
     2641 gtttaaccat atgacggttg cttatcagaa atccaaagtg ttggaagtcg gtggctatca
     2701 agatctgcaa gaagattact atttatggat caaactcatt gctcaattta aacaagtggc
     2761 aaacttgccg gatttattag tgtatgcccg agtcggtaac ggaatggttg gcagacgccg
     2821 tggaatggcc caagcagagg cagaatggcg tttatataaa ttaaaaaacc gctgccagat
     2881 tcacgatccg atttcaggtt ttgggatttt cataatgcgt gcaattcctc gtttattacc
     2941 ggtttcagca ctgaaaaaag tgtacggttt gctcagaaaa taaagctaaa aaaaccacag
     3001 attgctctgt ggttttttgt tattgattct tcaaaatctc attcattaat tgaatatcaa
     3061 tatggcagta gccgttcggg ttttttacta aatattcttg gtggtactct tcggcaggga
     3121 aatattgctc taacatttgg ttttccactg ccagcggctg atcaaattgg gtttgtaatt
     3181 gtgcaagtgc ggcggttacc accggttcgt cttgtgggtc ggtataataa ataccggtgc
     3241 ggtattgaat accgatgtcg ccaccttgtt tgttaatgct caccgggtct attactttaa
     3301 agaagtaatc tagtaatttt gccaagctaa tttgattggc atcataagtc acttttacca
     3361 cttcagcgtg tccgctaccg gcacacacct cttgatagct tgggttatcg gtattaccgt
     3421 tggcatagcc tgattgtgcc ttaatgacac ctttaatccg ttgcataaaa gattccacgc
     3481 cccagaaaca gccaccggct aagtaaattt cacgaatatt ttgcatatta cctctctata
     3541 attgtaaatt tttcgataaa tttgactgct tgtgatgaat aataaaatac cataatttag
     3601 tttttgtcaa attttgtctt gaatgatgat aatttcacaa gattttttgc ctaaaaatag
     3661 gtatagtatc gtcacctttt ttaatcaatt tataattaga ggttctatgg gtactcttaa
     3721 cagcttttta ggcattattg ttttactatt cattgcattt ttattttcga gcaataaacg
     3781 tgcaataaac gtgcgtaccg tattaggcgc attagcattg caggtagcta ttggtgcatt
     3841 ggtgctttat gtgccggcag ggcgtgatgc cttgaatgcg atggcaacag gtgtgtcaaa
     3901 agtgatttct tacggtaatg aagggatttc atttttattc ggtggcttag tcagcgacaa
     3961 aatgtttgaa gtatttggcg gcggcggctt tgtgttcgct ttccgtgtac ttccggtgat
     4021 tattttcttc tctgcactca tttctttgct ttactacatt ggagtgatgc aatgggtaat
     4081 ccgtatttta ggtggtttat tacaaaaagc attaggtaca tctaaagcgg aatctatgtc
     4141 tgcggcagct aatattttcg ttggccaaac tgaagctcca ttggtagtaa aaccttatat
     4201 cagcaaaatg acagaatctg aactctttgc gattatggca gggggcttag cttctattgc
     4261 aggttctgtt atggctggtt acgcaggaat gggcgtacca ttaacgtact taatcgcagc
     4321 ctcctttatg gcggctccgg cagggttatt attcgccaaa attatggtgc cacaaaccga
     4381 aaaattccgt gatgaactag aagcggtaga tttagaaaaa ccggcaaata ttcttgatgc
     4441 agcagcttca ggtgcttctt caggtatgca actggcatta aacgtagggg caatgttaat
     4501 tgcattcgtg gcgttaattg cattagtgaa tggtatttta ggaggtattg gcggctggtt
     4561 cggttatgat accttaaact taggtcaaat tttcggctgg gtatttaaac cgcttgcgtg
     4621 ggtaatcggt gtgccttggg aagaagcaga aattgcgggg cagatgatcg gcttaaaatt
     4681 agcggtaaat gaattcgtgg gttatttaga gtttgctaaa tacttagctc ctgagtcaac
     4741 cgtacattta acggataaaa ctgttgcgat tattaccttt gcactttgtg gctttgcgaa
     4801 cttcagctcg attgcgattt taatcggtgg tatcggggca atggctccaa gccgtcgtgg
     4861 tgatattgcc cgacttggga ttaaagccgt cattgctggc tcacttgcca acttaatgag
     4921 tgcaaccatt gccggcttgt ttatcggctt gggtggagca gttctctaat ccagtttgta
     4981 taaaaccact agaaatagtg gttttatctt tttttatctg acgtaaacgg ttgcttttgg
     5041 tttgtctatt tacaagcggt cagattttta taaaatttta ccaaccacca cgcatttaat
     5101 aaaaggagac actatgaatt taaaaaaatc cgctgagatt gctctttcgt tgatggatct
     5161 caccacatta aatgacaatg atactgatga gaaagtcatc gcattatgcc aacaagcgaa
     5221 caccaagttt ggcacaccgg cagcaatttg catttatcca cgttttatcc cgattgcccg
     5281 caaagcattg aaacagcaag gtatcgagca agtaaaaatt gcgacagtca cgaatttccc
     5341 tcacggtaat gatgatattg aaattgcggt agctgagacc aaagccgcag ttgcttatgg
     5401 ggcagatgag gtagatgtgg tattccctta taaagcatta attgcaggta atgagcaaat
     5461 aggtttcgac ttggtaaaac aatgcaaagc agtttgtgct gccaataatg tgttgcttaa
     5521 agtgattatt gaaaccggcg agttaaaatc agcagagctt attcgcaaag caagcgaaat
     5581 ttcgattcaa gcaggggctg attttattaa aacctcaacc ggcaaggtgg cagtgaatgc
     5641 tacccttgaa tctgcccgca ttatgcttga aaccattcgt gatttaggtg tggcagaccg
     5701 tgttggcttt aaagctgcag gtggggtaaa aacctcggaa gaagccgccc aatatcttgc
     5761 acttgcggaa tctattttag gcaatgactg ggtgagtgca tcacattttc gcttcggtgc
     5821 atcaagttta ttagctaatt tattagcaac attgaatgat gaacagcagc aagctgttca
     5881 aggttattaa ttaataaaga ggaacataaa atggctactc cacacattaa cgcacctgaa
     5941 ggtgctttcg ctgacgttgt aattatgccg ggcgatccgc ttcgtgctaa atatatcgct
     6001 gaaacctttt tagaagatgc aaaagaagtg accaatgttc gcaatatgtt aggctacacc
     6061 ggcacttata aaggtcgccg catttcagta atggggcacg gtatggggat tccatcttgc
     6121 tcgatttacg ctaaagaatt aattactgaa tatggcgtga agaaaattat ccgtgtcggc
     6181 tcgtgcggtg cggtacgtaa tgatgtcaaa gtgcgtgata ttgtcatcgg tgttggggct
     6241 tgtaccgatt ctaaagtaaa ccgtattcgc ttccgtgata acgattttgc ggcgattgct
     6301 gattttgata tgacattagc tgctcaccaa gcagctaaag cgaaaggcat tgaagctcgt
     6361 ttcggtaatt tattctcagc cgatttattc tactcacctg atgtcgaaat gtttgatgta
     6421 atggaaaaat acggcatttt aggcgtagaa atggaagcag caggcattta tgcggtagcg
     6481 gcagagtatg gagcaaaagc attagcaatt tgcaccgttt cagaccatat ccgcaccggt
     6541 gagcaaacta cctctgaaga acgtcaatta acctttaatg gtatgattga aattgcgtta
     6601 gagtcagtat taattggcga taagcagtaa tttctaacct gacaagcggt gaaattttgc
     6661 aaaaaatttg cagaatttga ccgcttgtta tttttgattc ttcctttatc ttcctttcgg
     6721 gctttcgcca aactgccttt tatattctcg actaaactgg cttgggcttt catagccgac
     6781 ttgataggcg gtttcactca cgcttactcc tgcttgtagt ttttcccttg ccgcatttag
     6841 tcttaacgat ttctgatatt gcagtggtgt cattgaagtt acgcttttaa aatgagcgta
     6901 aaaccccgcc acgctcattc ctacctcttc agccagtgct gacatattta gtgtatcggc
     6961 aaattttctt tctaagattt ttgttgcatt ggcaatttta gcaatattgc catcttgaga
     7021 gagtagagag cgtatatagc caccttgctc agattgtaat aaatagaaat agagttcgtt
     7081 taatatcaat ggtgcaagaa aatgaatatg atttggagaa ttaagcaaat ttagtaagcg
     7141 taacaaacag tctttaattt cattctccag ctcccattta gtgcgttgat taatgttttc
     7201 acttattttc tgtgggggaa gctgcgtgat aattttggca ataagatcaa aatccaagcg
     7261 tagtgaaatc gcaagataag gctgattttc tgcaactgat aaggtttggg taacaatcgg
     7321 caaattcaca gggcaaaaca tcatttcctg attaccgaag acttggcaat gtccattggt
     7381 acagatttgc cgctcgccta ataatactac acataaactt gggtcttgga tataaccgtt
     7441 aaatctaata gaacgatcgg cagcgtgaac ggttagccct gaaatgggag tagcccaaag
     7501 ttcatttttc ggagcgaggg caaaaataga ttcaataata gctcgatgag tttgcataat
     7561 aatctcactt ttagaaaatt aggcatattt tatagaaaat caggcaagac gacaaatagc
     7621 aaaaagcgga taatatcccc aatcaaataa agcatttaat aggagcaaaa tatgaaaatt
     7681 ttttatcaca catcagcaac tgcaaccggt ggtcgtgacg gcagaaccca agtggatgac
     7741 ggttctatcg gttttgattt agtcggtttc caaaatgaaa gtggcaaagt aggcactaat
     7801 ccggaacaat tattcgcaat gggctatgct gcctgctttg acagtgcaat gaatcacgtt
     7861 gccccaacct taggcattaa gccaaccaaa tccagtacca ctgttgcagt tgggattggt
     7921 caaaaagctg atggtgcttt cggcttagat ttagatatta ccattactgt ggaaggcatt
     7981 agtgcggaag aagcgaaaac tttaattagc aaggcacacg aagtttgccc atattcaaat
     8041 gcaacccgtg gtaatgtaga tgtgcgttta cacgttaaag tcgtgagtga atttgactta
     8101 taaaaaataa ataggggaaa tgaaaatgtt agattttgaa accacggtaa aaacacgaca
     8161 atcaatccgc aagtttttac cgcttccgat gacggaaact gaaatcaaac aaattcttgc
     8221 cgatgcacaa aatgcccctt cggcttgtaa tacacagccg tggttagtac acgttgcctc
     8281 tggtgaaacg ctagcacgct taaaagctcg ttttaaaaag acttttgaag cgggcattaa
     8341 tgatgcggat tttgagttca atcaaacgaa atttaccggt gactacgaac cacgttggcg
     8401 taaccaatac agccacgttt ttaccaccag ctatggcatt ccccgtgaag ataaagccgg
     8461 tcgccaagtg gtgtatgatg acaatattgt cggttatggt gcgccacata tggcattcct
     8521 atttatgcca agcattgacg gacacgatgt caatattgcc agcgatgttg gtatgtattc
     8581 gcaaactttc ttgctctcac tcaccgctcg tggttttggc ggcattccgc agcttgccct
     8641 tgcgatgttt gctaaagatg tgcgtgaaga gttaggcatt cctgaacatt acaagctcct
     8701 ccacgccatt tctttcggac acccagattg ggacgctatg cagaacaaac accatttagg
     8761 gcgtgtgccg gtggaagaga gtgcgacctt acattggtaa taaacaagcg gttgattttt
     8821 cgcaaaaatt tgcgaaatca accgtatttt ttaggagaat acgatgataa atttatttac
     8881 cccttatact ctgaataacg atgtagaaat taaaaaccgc cttgtagttg ccccaatgac
     8941 ccattccgcc tccaacgatg acggcacaat tagcgagcaa gaacgccatt tcttgcataa
     9001 tcgtgcagca gatatgggct tatttatcac cgcagcaacc ggggtaatgc gtaacggcaa
     9061 agcgttttac ggtcagccgg aagctcttga tgaacgctat ttggcaagcc tgacagaaac
     9121 tgccaatatc ttgaaaaatc aaggcaccaa agcgattttg caaattttcc acggtggggc
     9181 acaagctatt cctgccttgc ttgacggggc gaatgcagtt gcgccgtccg agcacgccaa
     9241 cggtgctaaa gccctaacgg cggacgaagt gaagcaaatg atccaagctt tcgctaatgc
     9301 caccgattta gctattcaag ccggttttga tggtgtggaa attcacgggg cgaacggcta
     9361 tttaattcaa caatttttct ctactgaatt taatcaacgc actgatgaat ggggcggtag
     9421 ccttgaaaaa cgcttgcgtt tcccgttagc ggtggttgag gcagcggtca gcgtgcgtga
     9481 aaaacaccaa cgccctgatt ttattatcgg ctaccgtttc tcaccggaag agccgggcga
     9541 acacggctta accatgaccg atacctacgc cctgattgat gctttgtgcg aaaagccgtt
     9601 gcaatacttg cacatttcac tctgggattt cggcaaaaaa gcccgccgtg gtgcagatac
     9661 caacttcacc cgtatgcaac tggtacacca acacatcaac gggcgtttac cgctgatcgg
     9721 cgtgggtaac ttattaaccg gtgaacagat ccgtaaagct tatgcaacag gctgggcaga
     9781 atttatcgcc ttaggtaaag cggtaatgct caacccgaac gtggcgacct tgttgaaaga
     9841 taatcgagac agtgagattg tcagcgaact cgacccgcaa caagccgacc actacggcat
     9901 tcccgataga ctatggggct actgcgtgca aggtggtgct tggttaccgc cgcttaaagg
     9961 gcaaaacggg caaccaatgg atatttaatc attacagaca agcggtcatt tttttgtaaa
    10021 aatttgcaat taagtgaccg cattttttca attatctgaa ggagaaaaat tatgattacg
    10081 ttacattacc tgaacagttc ctgctctcac cgcatcgttt ggttgttgga agccttaggc
    10141 ttggattacg agctaaaaat ctaccatcgt gaagagagcg gttttgcacc tatggaggta
    10201 aaagccttgc acccacttgg caaagcccct gtgttgcaag atggcgattt ggtgcttgcc
    10261 gaaggcaatg cgattatcca gcatctgatt gcccgttacg ccgaagggaa atttgagccg
    10321 actcgtggta gcgaagccta ttcaatgtat cactactggt tgtcgatttc tgcttcatta
    10381 ttctcggcaa accttgtgac aatgttggct aatcgctctg atctcggagc ttttggcgac
    10441 tacgcaaaag cgcagactcc gctgtatttt aaccatattg agcaaacact ttcagataaa
    10501 aaatggattg tgggtgatga actaaccggt gcggattttt ctctctgttt cccactacaa
    10561 tgggggcgta attttgttga aatggcaaat tacccgaata ttgcacgcta tttagaacag
    10621 gtggaaaatc accctgctta ccaaaaagcg aatcaaaaag ccctgaagct tgatatgaat
//
LOCUS       DANLRK010000088        10604 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.088, whole genome shotgun sequence.
ACCESSION   DANLRK010000088 DANLRK010000000
VERSION     DANLRK010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 10604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10604
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.088"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            111..2678
                     /gene="clpB"
                     /locus_tag="PXA33_001603"
     CDS             111..2678
                     /gene="clpB"
                     /locus_tag="PXA33_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HDL5120922.1"
                     /translation="MNFEKMTTKLQEALAESQSLAVGKDNPYIEPAHLLYALLKQEGG
                     SIASLFTTLNVDVPTLIRELQQILDRLPKVQGGNTQVSQQLVRLLNQSDKLAQQFGDS
                     FISSELFVLAALDDNGDLGKLFKQFGLNKEKLTQAISQIRGGDTVNNQNAEDTRQALK
                     KYTIDLTERAKAGKLDPVIGRDEEIRRAVQVLQRRTKNNPVLIGEPGVGKTAIVEGLA
                     QRIVNGEVPEGLKNKRVLSLDMGALIAGAKYRGEFEERLKAVLNDLAKEEGQVILFID
                     EIHTMVGAGKTDGAMDAGNLLKPSLARGELHCVGATTLDEYRQYIEKDAALERRFQKV
                     LVDEPTVEDTIAILRGLKERYEIHHHVQITDPAIVAAATLSHRYVSDRQLPDKAIDLI
                     DEAASSLRMEIDSKPEPLDKLERRIIQLKLERQALQKEEDEASRQRLAKLDEEMAAKE
                     REYSELEEIWKAEKSALLGTQHIKSELENARIAMDQARRENDFEKMSELQYGVIPNLE
                     KQLQAVENGEKNADAQLLRTKVTEEEIAEVLSKATGIPVAKMMEGEKEKLLRMEEVLH
                     ARVIGQNEAVDAVANAIRRSRAGLSDPNKPIGSFLFLGPTGVGKTELSKTLANFLFDD
                     PDAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRHPYSVVLLDEVEKAH
                     PDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSHLIRENAYKGYDVVKEMVM
                     EVVGQHFRPEFINRIDETVMFHSLGQEHIRSIAKIQLQRLIQRLAERGYEVTVTDAAL
                     DHIGKAGFDPLFGARPLKRAIQQELENPLAQQILSGKLLPNKAVTVDYQNGQIVATQ"
     gene            2892..4034
                     /locus_tag="PXA33_001604"
     CDS             2892..4034
                     /locus_tag="PXA33_001604"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HDL5120923.1"
                     /translation="MSQLFPEATIRTQAPYRNDIVGSFLRTDALKKAREDFNQQKITR
                     DELREVTRQEVKKLVELQKQHGVKAVSDGEFSRTWWHLDFLAELDGMDWQETEVFNVN
                     FTGHKPKGQTVKIVGDIDFSESHPFVDAYRLLKEAAGDYPTKFTIPAPTMLHIICCVR
                     AEDYQPLPQYQDEQKLYDDIANAYIKAMHKFYDMGLRNLQLDDTSWGQFCAEDKRKEF
                     AARGFNLTDLAEKYVALLNRIVDAKPADMSITMHICRGNFRSSWFSEGGYGPVAEVLF
                     GKCRVDGFFLEYDTERAGTFEPLRHIKDQQVVLGLITSKFGDLEDKNVIIERIKEAAQ
                     IVPINQLCLSPQCGFASTEEGNILTEAAQWAKLNLIKEIAEEVWGS"
     gene            complement(4091..5482)
                     /locus_tag="PXA33_001605"
     CDS             complement(4091..5482)
                     /locus_tag="PXA33_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HDL5120924.1"
                     /translation="MENTPLIEPSKNTRNSLIFVADAILFIILLNTLPFTPEANKGLA
                     LLIFIAVLWLTEALHVTVTALLIPILAVALGLVKSKEALVAFADPTIFLFFGGFALAT
                     ALHIQQLDRMIANKIMALAKGKLSIAVIYLFAVTAFLSMWMSNTATAAMMLPLAMGIL
                     SKLDQKQNHNTYLFVLLGIAYSASIGGMGTLVGSPPNAIVASQLNLTFADWLKYGLPI
                     MLILFPLMIATLYIVFKPNFSVSFDRSFEKIELNRSRIITLAIFVFIALGWIFGDKIN
                     PIISAFLGINGKIASFDTILALIAAALICITRVANWQQIQENTEWGVLFLFGGGLTLS
                     AVLGQTGASKIMADGVVSLIEGGHFYLIGLIVAAFIIFLTEVTSNTASAALLVPIFIS
                     IAQALNMPPVGLALIIGLGASCAFMLPVATPPNAIVFGTGQIKQSEMIKVGFWLNIIS
                     IFVIATVGYLFWL"
     gene            complement(5642..6541)
                     /gene="cdd"
                     /locus_tag="PXA33_001606"
     CDS             complement(5642..6541)
                     /gene="cdd"
                     /locus_tag="PXA33_001606"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HDL5120925.1"
                     /translation="MFRTDITERFEQLKAAKNCEVTQAIIEQLIKQDFHGQLSYEVVD
                     ELCEKFKRSRVELALYCIAIAACYAVTPVSDFNVGAVAIGKNGDFYFGANQEFSGECM
                     QQSVHAEQSAISHAFLAGETLITDVVVNYTPCGHCRQFMNELNSAASLKVHLPHSQNN
                     LLHSYLPDSFGPKDLGIEKVLFDEQPQQFEVKGDHLQQAAIFAANNAYAPYSNAFSGV
                     ALQVGERIVCGKYTENAAFNPTFLPLQSALNYRLFLGLADIPVTRVVLAEAKGSLSSY
                     AMTQALAKSLFGLEIEYLSLQAV"
     gene            complement(6555..7457)
                     /locus_tag="PXA33_001607"
     CDS             complement(6555..7457)
                     /locus_tag="PXA33_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDL5120926.1"
                     /translation="MISSSLKLGYFSLIVATLFWGGNFVLGKVLSGAVQPITLTYLRW
                     LPAFLILVLLFFRPTWRVMHLLKPRLGVMWVLGLLGVILFPATLYEGLKTTTALNASL
                     YLAISPVLVLFLNRLVFKEKISPIILSGAIISLLGVLWLLMQGKLTRLFSLAINQGDL
                     WAIASTISWAVYCCVIRLRPQGISNTVFLTVLVGLAVLTLTPFFIYEYVAESAKNLAN
                     LTALQWSGIGYLVIGPSILSYAFWNFGIAIVGSAKGAVMTNFTPLFAAMFSIILLNES
                     VHTYHIISAILITAGILICSYKKH"
     gene            complement(7454..7810)
                     /gene="folB"
                     /locus_tag="PXA33_001608"
     CDS             complement(7454..7810)
                     /gene="folB"
                     /locus_tag="PXA33_001608"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HDL5120927.1"
                     /translation="MSDKVFIRELTAFASIGAYNWEHTIKQRLVFNIDMAWDFSKAAE
                     TDDVQYCLNYAEVSQKVLDFVESQPFKLVETVAYQLAELLQKEYQLSWIRIELHKPKA
                     VAQASSVGVIVERGHQ"
     gene            7880..8485
                     /gene="plsY"
                     /locus_tag="PXA33_001609"
     CDS             7880..8485
                     /gene="plsY"
                     /locus_tag="PXA33_001609"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HDL5120928.1"
                     /translation="MNLIPYLLILTAYLLGSISSAVIFCKLAGLPDPREVGSHNAGAT
                     NVLRIGGKSAALGVLLFDILKGTLPVAMGIYFELSPSQLGFIALAVCLGHIFPIFFQF
                     RGGKGVATAFGAISALSFIVAGGAIGTWLLVFLISGYSSLSAVFTALVVPFYIWWFEP
                     QFTFPVALVCCLLVYRHHDNIQRLWRGQEDKLWDKFKKNRA"
     gene            complement(8559..10466)
                     /gene="parE"
                     /locus_tag="PXA33_001610"
     CDS             complement(8559..10466)
                     /gene="parE"
                     /locus_tag="PXA33_001610"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HDL5120929.1"
                     /translation="MSEKRYGADEITVLKDLEPVQLRPGMYTDTTRPNHLGQEVIDNS
                     VDEALSGFADKIDVILHADNSLEVVDNGRGMPVDIHSTEKISGVELILTKLHAGGKFS
                     NKNYTFSGGLHGVGISVVNALSERVEIQIKRGGEVYSIAFANGVKVEDLTVIGTCPKK
                     QTGTTVRFYPNPKYFDSPRFSVSRLRHLLRAKAVLCPKLTINFIDKINNTEESWYYED
                     GLSDYLSEALNGYETVPNPPFIGDVTAETEAVSWALTWLPEGGELVAESYVNLIPTAQ
                     GGTHVNGLRNGLLKAMVEFCEIHNLLPKGVKLTADDVWNRCAYVLSLKIQEPQFAGQT
                     KERLSSRQASSYVDSTLKDAFSLWLNQNVQTGKLIAEMAISSAQSRMRAAKKVVRKKL
                     VSGPALPGKLADCTAQDLSRTELFLVEGDSAGGSAKQARDKEYQAILPLRGKILNTWE
                     VSSDQVLASQEVHDIAVALGIDPDSDNLEELRYGKVCILADADSDGLHIATLLCALFL
                     RHFPKLVQNGHVYVAMPPLYRIDIGKDEVHYALDEAEKEAILARLAKKKGKPNVQRFK
                     GLGEMNPSQLRETTMEPSTRRLVQLTFDDTVEEGELNTFEIMDMLLGKKRAEDRKQWL
                     QNRGDEADLAV"
BASE COUNT         3296 a         2327 c         2118 g         2863 t
ORIGIN      
        1 atctttttca catttttact tgaaaattgt ttaccaagct ccatttagtg tttcaacaag
       61 agatgtaaat aaaaatttac actttctaaa ctcattgtaa ggagaaagaa atgaattttg
      121 aaaaaatgac cacaaaactg caagaagcct tggctgaatc acagtcgctt gcggtaggaa
      181 aagataatcc gtacatcgaa ccggcacatt tgctctatgc gttgttaaaa caagaaggtg
      241 gctcgattgc ttctttgttt accacattaa atgttgatgt accgacttta attcgtgaat
      301 tacaacaaat tttagaccgc ttgccgaaag ttcaaggcgg taacacccaa gtttcacaac
      361 agttggtcag actgttaaat caatcagaca aactggcaca acaattcggc gacagcttta
      421 tttcatcaga actatttgtt ctagctgcac ttgatgacaa tggcgatttg gggaaactat
      481 ttaaacaatt tggcttaaac aaggaaaaac taacacaagc catcagccaa attcgaggag
      541 gagacacagt gaataatcaa aatgcggaag atacccgcca agcactcaaa aaatatacga
      601 ttgatttaac cgaaagagcc aaagccggta aattagaccc ggttatcggg cgtgatgaag
      661 aaattcgccg tgcggtgcaa gtcttacaac gccgtaccaa aaacaaccct gtgttaattg
      721 gtgaaccggg tgtggggaaa acggcgattg tagaaggctt ggcacagcgt attgtaaacg
      781 gcgaagtgcc ggaaggcttg aaaaataagc gtgtactttc gctggatatg ggagcattaa
      841 ttgccggtgc gaaatatcgt ggtgaatttg aagagcgttt aaaagcggtt ttaaacgacc
      901 tggcgaaaga agaagggcaa gttatcctct ttattgatga aattcacacg atggttggag
      961 caggcaaaac agacggagca atggacgccg gcaacttatt aaaaccaagc cttgcccgtg
     1021 gtgaattgca ctgtgtgggg gctacaaccc ttgatgaata tcgccaatac attgaaaaag
     1081 acgcagccct tgaacgccgt ttccaaaaag tattggtgga cgaaccaacg gttgaagata
     1141 cgattgcgat tttacgtggt ttgaaagagc gttatgaaat ccatcatcac gttcaaatta
     1201 cagaccctgc catcgtggcg gcggcaacgc tttcgcaccg ttatgtgtct gaccgtcaat
     1261 tgccggataa agcgattgac ttgattgatg aagccgcttc aagcctgcgt atggagattg
     1321 attctaaacc tgagccgttg gataaattag aacgccgcat tatccaactc aaattggaac
     1381 gccaagcgtt acaaaaagag gaagatgaag cgagccgtca gcgtttagcc aaattagacg
     1441 aagaaatggc agcgaaagag cgtgaatatt ccgagctgga agaaatctgg aaagcggaaa
     1501 aatccgcctt acttggtaca cagcatatta aatcggagtt ggaaaatgcc cgaattgcga
     1561 tggatcaggc tcgccgtgaa aacgatttcg agaagatgtc ggaattgcaa tatggcgtga
     1621 ttccaaactt ggaaaaacag ttacaagcgg tcgaaaatgg cgaaaaaaat gcagatgctc
     1681 agctcctccg tactaaagtc accgaagagg aaattgcgga agtgctttca aaagcaacgg
     1741 gcattcctgt tgcaaaaatg atggaaggcg agaaggaaaa actccttcgt atggaagaag
     1801 tattacacgc tcgggttatc gggcaaaatg aagccgttga tgcggtggca aatgcgattc
     1861 gccgtagccg tgccggactt tccgatccga ataaaccgat tggttcattc ctattcttag
     1921 gaccgaccgg cgtgggtaaa accgagttaa gcaaaacctt agctaatttc ttgtttgacg
     1981 atcctgatgc tatggtgcga attgatatgt ctgaatttat ggaaaaacac agcgtatccc
     2041 gcttagtggg cgcacctccg ggctatgtcg gctacgaaga aggcggctat ttgaccgaag
     2101 cggttcgccg ccatccatat tcggtagtgt tacttgatga agtggaaaaa gctcaccctg
     2161 atgtgtttaa cattttattg caagtgttag atgacgggcg tttaaccgac gggcaaggca
     2221 gaacagtcga tttccgcaat acggtagtga ttatgacctc aaacttaggc tcgcatttaa
     2281 ttcgagaaaa tgcgtataaa ggctatgatg tggttaaaga aatggtgatg gaagtcgtcg
     2341 gacagcactt ccgccctgaa tttatcaacc gtattgatga aacggtgatg ttccactcac
     2401 ttggtcaaga gcatatccgt tcgattgcga agattcagct acaacgctta atccaacgct
     2461 tggcagaacg aggctatgag gtgacagtaa ccgatgcagc attagatcat atcggcaagg
     2521 ctggtttcga tccgctgttc ggagcaagac cactgaaacg tgcgattcaa caagagcttg
     2581 aaaatccgct tgctcaacag atcttaagcg gtaaattatt accgaataaa gccgttacgg
     2641 tggattacca aaacgggcag attgttgcaa cacaataatg tctccgatgg tgcgagcgtc
     2701 ctcgctcgta ccttacaagc ggtggattta tcgtaaaaat ttgcaaaaaa ttgctcgaaa
     2761 ataaccgctt gcacagtaaa aattttcata ttgcccttca ttaattttca taagctaatg
     2821 aagggctttt tattactctt tttgctatca ttaacggcgt ttataaccaa tacaattaca
     2881 aggagtaatt tatgagccaa ttatttccgg aagcaacaat ccgcactcaa gccccgtatc
     2941 gtaatgatat tgtaggcagc tttctacgta ccgatgcgtt aaaaaaagcc agagaagatt
     3001 tcaatcagca aaaaatcacc cgtgacgaat tgcgtgaagt cacacgccaa gaggtcaaaa
     3061 aattagttga gttgcaaaag caacacggtg taaaagcagt ctctgacggt gaatttagcc
     3121 ggacttggtg gcatttagat ttcttagctg aacttgatgg aatggattgg caagaaaccg
     3181 aagtctttaa cgtgaacttt accggtcata aacccaaagg gcaaacggta aaaatcgtgg
     3241 gcgatattga tttctccgaa agccaccctt ttgtggacgc ttatcgtctt ttaaaagaag
     3301 ctgccggcga ttacccaacc aaattcacga ttccggcacc gacaatgtta catattatct
     3361 gctgtgtgcg tgcggaagat taccaaccgt taccacaata tcaagacgaa caaaaactgt
     3421 acgatgatat tgctaatgct tatatcaaag caatgcacaa attctacgat atggggctac
     3481 gtaacttaca attagacgac accagttggg gacaattctg tgccgaagat aaacggaaag
     3541 agtttgcagc cagaggattt aatttaactg atttagcgga aaaatatgtc gcattattaa
     3601 accgtattgt tgatgcaaaa ccggcggata tgtcaattac gatgcacatc tgccgtggta
     3661 acttccgttc ttcttggttc tcggaaggcg gttatggacc ggttgccgaa gtattattcg
     3721 gtaaatgccg tgttgacggc ttcttcttag aatacgatac cgaacgtgca ggcactttcg
     3781 agccactacg ccacattaaa gatcaacaag tggtactagg cttaattacc tctaaatttg
     3841 gcgatttaga agataaaaat gtgatcattg agcgtattaa agaagcggct caaattgtgc
     3901 caatcaatca gctatgctta tcaccacaat gcggctttgc ctccaccgaa gaaggcaata
     3961 ttttaaccga agccgcacaa tgggcgaaac taaacttaat taaagaaatt gcggaagaag
     4021 tttggggtag ctaatcctct ataatacaaa tagccgagta aatactcggc tattttagtt
     4081 tgttttaaca ttacaaccag aagagatacc caacagtcgc aatcacaaag atagagataa
     4141 tatttagcca gaaaccaact ttaatcatct cactttgttt aatttgccct gtaccaaata
     4201 caatcgcatt cggtggggtg gcaaccggca acataaaggc gcaagatgct cctagaccaa
     4261 taattaaggc taatccgacc ggtggcatat ttaatgcttg tgctatagag atgaaaatcg
     4321 gcactaacaa agccgcactg gctgtgttag aggtaacctc agttaggaaa ataataaatg
     4381 ctgccacaat taaaccaatt aggtaaaaat gccccccttc aattagtgag acgaccccat
     4441 cagccataat tttgctcgca ccagtttgtc ctaatacagc acttaacgtc aaaccaccac
     4501 caaataggaa taataccccc cactcggtgt tttcttgaat ctgttgccaa tttgcgacac
     4561 gagtaatgca aataagtgct gcggcaatca atgcaagaat ggtgtcaaag ctcgcaattt
     4621 tgccattaat tcccagaaat gcagaaataa tagggttaat tttatcacca aaaatccaac
     4681 caagcgcaat aaagacgaaa atagccaagg tgataatacg agaacgattt aattcaattt
     4741 tctcaaatga tcgatcaaaa ctaactgaaa aatttggttt aaatacgata tatagtgttg
     4801 caatcattaa tgggaataaa attagcataa ttggcaaacc atattttaac caatcagcaa
     4861 aagtcagatt caattgagaa gcaacaatcg cattcggtgg gcttcccact aaagtaccca
     4921 ttccaccaat actggcacta taagcaattc ctaataacac gaaaagataa gtattatggt
     4981 tttgtttctg atcaagttta cttaaaatcc ccattgccaa aggtaacatc attgcagctg
     5041 tagctgtatt actcatccac attgacaaaa acgctgttac cgcaaataaa taaataactg
     5101 caatcgataa tttaccttta gctaatgcca taattttatt ggcaatcata cgatctagct
     5161 gctgaatgtg taatgctgtc gctaaggcaa agcctccaaa aaataagaaa atcgttggat
     5221 cagcaaaagc aacaagagct tcttttgatt taaccagccc cagtgctaca gccaaaatcg
     5281 gaattaataa tgccgtaacc gtaacgtgta atgcttctgt tagccataag acggcaatga
     5341 aaattaacaa tgccaacccc ttattggctt caggggtaaa aggcaaggta tttaacaaaa
     5401 taataaataa aatagcatct gcaacaaaaa tcaagctatt gcgtgtattt ttactcggtt
     5461 caataagagg agtattttcc ataaaaacct cacaaaatat agataatcag tggtatcact
     5521 atatataaaa aaacgatata ggcaaatata attgttgcaa aagtgtgaag ctcatcaaaa
     5581 aatttaacac taaaaaataa attgcttaaa gatacagcac ctttgcaaaa ataaatcaaa
     5641 attacaccgc ttgtagcgaa aggtattcaa tctctaaccc aaatagtgat tttgccaatg
     5701 cttgagtcat tgcataacta ctgagagagc ctttcgcctc agccagcaca actcgagtaa
     5761 cgggaatatc ggcaagccct aaaaataatc gatagttcaa cgcgctttgc aacggtaaaa
     5821 aagtaggatt aaaagctgca ttctcagtat atttaccaca aacaatacgc tcacctactt
     5881 gcagtgcaac cccactaaat gcgttgctgt aaggggcata agcattattg gcagcaaaaa
     5941 ttgccgcttg ttgtaaatga tcgcccttaa cctcaaattg ttgcggttgt tcatcaaata
     6001 agactttctc aatccctaaa tctttcggtc caaaactatc cggcaaatag ctatggagca
     6061 aattattttg gctatgcggt aaatgtactt ttaagcttgc cgcactgttt aattcattca
     6121 taaattggcg gcaatgccca caaggggtat agttcacaac cacatctgtt atcaaagttt
     6181 cccctgctaa aaatgcgtgc gagattgcac tttgctctgc gtgaacggat tgttgcatac
     6241 attccccgga aaactcttgg tttgccccaa aatagaaatc tccgtttttg ccaatcgcaa
     6301 ccgcccctac attaaaatcg gaaactggtg taaccgcata acaggcagca atggcaatgc
     6361 aatataaagc caactcaaca cgagagcgtt taaatttttc acagagttca tcaaccactt
     6421 cgtaagagag ctgcccgtga aaatcttgct tgataagttg ctcaataata gcttgtgtaa
     6481 cttcacagtt tttcgctgct tttaattgtt caaaacgctc tgtaatgtct gttcgaaaca
     6541 tactgtacct ccgtttaatg ttttttatag ctgcaaatca gtatccctgc cgtaatcagt
     6601 atggcactaa taatatggta agtatgaact gattcattta ataaaataat actgaacatt
     6661 gctgcaaaga gcggcgtaaa attagtcatt accgctcctt tagcagaacc gactattgca
     6721 atgccgaaat tccaaaacgc ataagagaga atagaagggc cgataaccaa atagcctatc
     6781 ccactccatt gtaaagcggt caaatttgct aaattttttg cagattctgc tacatattca
     6841 tagataaaaa atggcgttaa cgttagaact gccagcccca ctaatacggt caaaaacacc
     6901 gtattgctta taccttgtgg acgtagccga atcacacaac aataaaccgc ccagcttata
     6961 gtagaagcaa tcgcccataa atcaccttga ttaattgcca agctgaataa gcgagttagc
     7021 ttcccttgca tcaatagcca cagcactccc agcaaactga ttattgcccc acttagaata
     7081 atcgggctga ttttctcctt aaaaacgagc cgatttaaaa atagcaccaa tacaggcgaa
     7141 atagctaaat agagactcgc attcaatgct gttgtcgttt ttaaaccttc atataaggta
     7201 gccgggaata aaattacccc aagcagacct aatacccaca tcacacctaa acgaggtttt
     7261 aataagtgca ttacccgcca agttgggcga aaaaagagca aaactaaaat taaaaaagcc
     7321 ggcaaccaac gcaaataggt taaggtaata ggctgaactg ccccacttaa cactttgcct
     7381 aatacaaaat tcccacccca aaaaagagta gcaacgatta aactgaaata ccccaatttc
     7441 agtgatgatg agatcattga tgacctcgtt ctacaatcac acctacactg cttgcttggg
     7501 caaccgcttt cggcttatgc agttcaatcc gaatccaaga aagttgatac tctttttgta
     7561 atagttccgc cagttgatag gcaacagttt caactaattt gaaaggttga gattcaacaa
     7621 aatccagcac tttttgcgat acttccgcat aattcaagca atattgtaca tcatcggttt
     7681 ctgccgcttt ggaaaaatcc cacgccatat caatgttaaa aaccaagcgt tgcttaatcg
     7741 tatgctccca attgtaagcc ccaatggaag caaaagcggt tagttctcga ataaaaacct
     7801 tatcactcat ttttcgtcct gttttatttt ctgaatttct gtaaaatata gcacaagata
     7861 aatttaatca ggaaaattta tgaatttaat tccctattta ttgatcttaa ccgcctattt
     7921 gctcggctcg atttccagtg cggtcatttt ttgtaagctg gcaggcttgc ctgacccaag
     7981 agaagtcggc tcacacaatg ccggtgcaac gaacgtacta cgaattggcg gtaaaagtgc
     8041 cgcattaggt gtgcttttat tcgatatttt aaaaggcaca ttaccggtgg cgatggggat
     8101 ttattttgaa ctctctccct cacaactcgg tttcatcgcc ttagcggttt gtcttgggca
     8161 tattttcccg attttcttcc aattccgtgg cggaaaaggc gttgccaccg catttggtgc
     8221 aatctccgca ttaagcttta ttgttgcagg cggtgctatc ggcacttggc tactggtgtt
     8281 tttaatctcc ggctattctt ctttaagtgc cgtcttcact gccttggtcg taccgtttta
     8341 catttggtgg ttcgagccac aatttacttt ccccgtcgcc ttagtttgtt gcctattggt
     8401 ttaccgccat cacgataata ttcaacgctt atggcgtggg caggaagaca aactgtggga
     8461 taaattcaag aaaaatagag cataaaaata ggcatagtga accgttcgct atgcctattt
     8521 gcaaattatt gcggaaaaat caccgcttgt cttctcgact aaaccgctaa atctgcttca
     8581 tctcctcgat tttgtagcca ttgtttacga tcttccgctc gttttttacc caatagcata
     8641 tccataattt caaaggtatt caattcgccc tcttctaccg tgtcatcaaa ggttaattgt
     8701 actaagcggc gagtgcttgg ctccatcgta gtttcacgca actggctcgg gttcatttcg
     8761 cctaagcctt taaagcgttg cacatttggc tttccttttt tcttggcaag gcgagctaaa
     8821 atcgcctctt tttccgcctc atctaaggca taatgcacct catctttgcc gatatcaatg
     8881 cgataaagtg gtggcattgc cacataaacg tgtccgtttt gcaccaattt cgggaaatga
     8941 cgaaggaata acgcacacag caaggtggca atatgcaaac cgtctgaatc cgcatcggca
     9001 agaatacaaa ctttgccata acgcaactct tctaaattat cgctatcagg gtcaattccc
     9061 aaggctaccg caatatcgtg tacttcttgt gaagccaata cttggtctga tgaaacctcc
     9121 caagtgttta aaatcttacc acgcagtggc aaaatggctt gatactcttt atctcgagct
     9181 tgttttgccg aaccgccggc agaatcacct tctaccaaga aaagttcggt acggctgaga
     9241 tcttgagctg tacaatccgc cagtttgccc ggtaaagccg gaccactcac caatttctta
     9301 cgcaccactt ttttcgccgc acgcatacgg ctctgggcgg aactaattgc catttcagca
     9361 attaatttac ctgtctgcac attttggtta agccataaac taaaggcatc tttcaaggtg
     9421 ctatccacat agcttgaggc ttgacgggaa gacaaacgct ctttggtctg ccctgcaaat
     9481 tgtggctctt gaattttcag agaaagcaca taagcacaac ggttccacac atcatcagcg
     9541 gttaatttta cccctttcgg caataaatta tggatttcgc aaaactccac catcgccttt
     9601 aataaaccgt tacgcaagcc attaacgtgc gtgccgcctt gtgcggtcgg aatcaggttc
     9661 acatagcttt ccgctactaa ctcgccccct tccggcaacc aagttaatgc ccagcttacc
     9721 gcctctgttt cagcagtaac atcaccaata aacggtggat tcggcacggt ttcataaccg
     9781 tttaatgcct cgctcaaata atcagataag ccgtcttcgt aataccaact ttcttcggta
     9841 ttgttgattt tatcaataaa attgatggtg agtttcgggc aaagcaccgc tttggctcgt
     9901 aacaaatggc gtaaacgact caccgaaaaa cgagggctat caaaatattt tgggttcggg
     9961 taaaaacgca ctgtcgtgcc ggtctgtttt ttcgggcaag taccaattac cgtcagatct
    10021 tccactttta ccccgttggc aaaggcaatc gaatagactt ctccgccacg tttaatctga
    10081 atttcaaccc gttcggacag ggcatttacc accgaaatcc ccaccccgtg caaaccaccg
    10141 gaaaacgtat agtttttatt ggaaaattta ccgccggcgt gcaatttcgt taagataagc
    10201 tccacccctg aaattttctc ggtggaatga atatcgaccg gcattccccg cccgttatcc
    10261 accacttcca acgaattatc agcatgaaga atcacatcaa ttttatctgc aaagccggac
    10321 aacgcctcat cgacactatt atcaatcacc tcctgcccca aatggtttgg acgtgtggta
    10381 tccgtgtaca ttcccgggcg aagctgcacc ggctcgaggt ctttcagaac ggtaatttca
    10441 tccgccccat agcgtttttc agacattatg ctttctctta ggcttaaaat aaaataaggc
    10501 tgaattttaa ggaatattca gctattttgc aaaattttgg tgaaaaatga cctgttgtta
    10561 tgcaataaaa actatttttg cgatctacat cgaaaaaatg agtt
//
LOCUS       DANLRK010000089        10489 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.089, whole genome shotgun sequence.
ACCESSION   DANLRK010000089 DANLRK010000000
VERSION     DANLRK010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 10489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10489
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.089"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            27..1535
                     /locus_tag="PXA33_001611"
     CDS             27..1535
                     /locus_tag="PXA33_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012478468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HDL5120930.1"
                     /translation="MSKCPVTHLTMNNGAPVVDNQNSLTAGPRGPLLAQDLWLNEKLA
                     NFVREVIPERRMHAKGSGAFGTFTVTNDITKYTRAAIFSQVGKKTEMFARFTTVAGER
                     GAADAERDIRGFALKFYTEEGNWDMVGNNTPVFFLRDPRKFPDLNKAVKRDPRTNMRS
                     ATNNWDFWTLLPEAFHQVTIVMSERGIPASYRHMHGYGSHTYSFINAANERFWVKFHF
                     HTQQGIKNLTNEEAAAIIANDRESHQRDLYEAIERGDFPKWKMFVQIMPETDADKVPY
                     HPFDLTKVWPKSDYPLIEVGEFELNRNPENFFADVEQSAFAPSNLVPGISVSPDRMLQ
                     ARLFNYADAQRYRLGVNHHQIPVNAPRCPVHSNARDGQGRVDGNYGSTIHYEPNSFGK
                     WQEQAQYAEPPLKINGDAAHWNYREDDADYFSQPRVLFNMMNDEQKQALFNNTAAGMG
                     DALDFIKYRHIRNCYACDPAYGEGVAKALGMTVEDAIAARATDPALGQPGLL"
     gene            complement(1600..1821)
                     /locus_tag="PXA33_001612"
     CDS             complement(1600..1821)
                     /locus_tag="PXA33_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HDL5120931.1"
                     /translation="MNVRLPASGLVCPFPLVEAKAAMAKLNKGDSLTIEFDCTQATEA
                     IPNWAEEEGYEVTDYQQLGDALWEITVVK"
     gene            complement(1850..2359)
                     /locus_tag="PXA33_001613"
     CDS             complement(1850..2359)
                     /locus_tag="PXA33_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HDL5120932.1"
                     /translation="MLFTGLLCGFLLGFVMQRGRFCITGAFRDLYVTKNSRMFVALLI
                     AITVQSIGIWLLYEAGSFSSPAEDLPLLAVIIGAFLFGIGIIYAGGCATGTWYRAGEG
                     LIGSWVALVIYGLFSASMRTGVLAPLNQELKSNVIQHRTIYETFGISPWVLVFILSVI
                     TLALSAHSI"
     gene            complement(2466..3800)
                     /locus_tag="PXA33_001614"
     CDS             complement(2466..3800)
                     /locus_tag="PXA33_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HDL5120933.1"
                     /translation="MQLYSYICLLFGLAMFISFFTRKLNENIQTTIAITASALVGSLL
                     ILTFGSLGWFNVDKLAISIFEQLDFKSFLLNGMLGFLLFAGSLGIKLPLMKEQRREIA
                     VYALFSTLVSTFLIGALIYGVARLLGLELGFVYCLLFGSLISPTDPIAVLAIIKSLKA
                     PKRLSMHVEGESLFNDGIGLVIFTTIFAVAFGGQEPNLSGITGLFLQEAVGGIVFGLL
                     TGFVAHKFISATDDGSLEILITLTIPTVGFVIANYLHVSGALTMVVAGIMIGNWTRRS
                     GFSEQSQKYLDHFWEMIDHSLNYLLFLLIGLSTLLVDFTFIGGVLMLAAIPICLLARY
                     ISLWIPYQVLKRFRTYNPYTLKIMTWGGLRGGLALAMALSVPAGAAVVSSSEGNLDVR
                     DLILLMTYAVVTFSILVQGSTVEPMIKKSKLVDPRKVALNKLGAPDDVIPHI"
     gene            complement(3911..5059)
                     /gene="dapE"
                     /locus_tag="PXA33_001615"
     CDS             complement(3911..5059)
                     /gene="dapE"
                     /locus_tag="PXA33_001615"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HDL5120934.1"
                     /translation="MKNEIISLAQNLIRRDSISPQDKGCQQEIAQRLEKLGFNIEWLP
                     FGDTLNLWATHGSENGKTEPCIVFAGHTDVVPVGDESQWAYPPFSAEIVDGMLYGRGA
                     ADMKGSLSALVVAAETFVQHHPNHKGKVALLITSDEEAAAKDGTVKVVETLMARNEAV
                     HYAVVGEPSSSKVFGDVIKNGRRGSITADLYIEGIQGHVAYPHLAENPVHTSLGFLTE
                     LTTYQWDNGNEFFPPTSLQIANIKAGTGSNNVIPGELYVQFNLRYCTEVTDEIIKAKV
                     AEMLEKHGLKYRISWNLSGKPFLAGNGKLVEAALQAVENVTKITPRLDTGGGTSDGRF
                     IALMGAEVVEFGPLNATIHKVNECVSADDLAHCGEIYYQILEKLLATA"
     gene            complement(5189..5536)
                     /locus_tag="PXA33_001616"
     CDS             complement(5189..5536)
                     /locus_tag="PXA33_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HDL5120935.1"
                     /translation="MTTLVYGIKNCDTVKKALKWLEDHNQNPQLHDYRVDGLNADWLV
                     EMEAKFGWENLVNKRSTTWRNLDEEIKNNLNRESALKVLTEQPTLIKRPIVIKEDIAL
                     IGFNEKEYVAAFN"
     gene            complement(5536..6393)
                     /gene="folP"
                     /locus_tag="PXA33_001617"
     CDS             complement(5536..6393)
                     /gene="folP"
                     /locus_tag="PXA33_001617"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HDL5120936.1"
                     /translation="MQIHFKNFQKNDRLLDLTTPQIMGILNFTPDSFSDSGKFFSLDK
                     ALFQAEKMLADGASIIDIGGESTRPNAAIVSLEQELERVVPVVEAIRSRFDCLISVDS
                     SKAEVFKESAKVGMDIINDIRALTEPNALAAAVELGLPVCLMHMQGNPQTMQQNPHYD
                     DVLEDVADFLNQRIFECITAGIPKEHIILDPGFGFGKSVKHNYQLLKHLKAFVDSGFP
                     VLAGLSRKSMIGAVLDKPVEQRLLGSVVGALLAAQNGAKILRVHDVAETADALKIWQA
                     MLESDNEEK"
     gene            complement(6502..7464)
                     /gene="rluC"
                     /locus_tag="PXA33_001618"
     CDS             complement(6502..7464)
                     /gene="rluC"
                     /locus_tag="PXA33_001618"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HDL5120937.1"
                     /translation="MTTEKVISAGVQFFTISEDEAGQRLDNFLLAKLKGVPKSLIYRI
                     VRKGEVRVNKGRNKPEYKLQPDDLVRVPPVRVAEKNEAPISTKLNKVAELESQILYED
                     ESMLVINKPSGIAVHGGSGLSFGVIEALRALRPQARFLELVHRIDRDTSGILLVAKKR
                     SALRSLHEQLREKVVQKDYLALVRGQWQAHTKVIKAPLLKNELASGERIVRVSEEGKP
                     SETRFSIEERYANATLVKASPITGRTHQIRVHTQYAGHPIALDDKYGDAEFDSKMKEV
                     GLQRLFLHAHAIRFEHPKTGEEMVITAPLDKTLKGVLAKLRANY"
     gene            7573..8475
                     /locus_tag="PXA33_001619"
     CDS             7573..8475
                     /locus_tag="PXA33_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HDL5120938.1"
                     /translation="MTEKVTETAPNIDVVAETEKVIHKVSNMNIETMLNEWIIPYGTK
                     ILLAIAIFVVGKFLARLISRLLGKAALKSTKDEMLQSFIVSISYFLLLLIVVIASLSQ
                     IGINTSSLVALIGAAGLAIGLSLQNSLQNFAAGVMLLIFKPFRKGDLIETGGMTGVVE
                     QMGLLVLELRTGDNKTVLLPNGKVFSDSIINYSNNPTRRIDFTFDISYESNLKEAKEI
                     IQRILDANTYVLKDPAPVVAVGALAPHSVQLVVRPWVQTPNYWAAYWEIIEQVKLAFG
                     EAGISIPYNQMELHIKSGSLENKA"
     gene            8573..9070
                     /gene="rraA"
                     /locus_tag="PXA33_001620"
     CDS             8573..9070
                     /gene="rraA"
                     /locus_tag="PXA33_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HDL5120939.1"
                     /translation="MRIDTSALCDLYSDQVDVVEPIFSSFGGASSFFGKITTVKCFES
                     NGLIAEVLEEEGEGRVLLVDGGGAVRRALIDAELAQLAVDNGWEGIIVYGAVRQLDIL
                     ERLDIGIHALAPIPVGADNTDIGEVDTPVNFGGVTFFPEDYVYADLTGIILSQELLDL
                     NDISE"
     gene            complement(9118..10479)
                     /locus_tag="PXA33_001621"
     CDS             complement(9118..10479)
                     /locus_tag="PXA33_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDL5120940.1"
                     /translation="MLFLNKIKQLFIFAFTLVLSACSLVVYKPVETIKQVNVERGYRQ
                     KNVIAESAKDGNIVVIMFSGGGTRAAALGYGVLEEFKKVRVKETNKGNTLLENIDIAF
                     GVSGGSILATYFALEGKDIIPKFADRFLKANFQKDIITEVFSLSNLPRLSSPQYGRGD
                     LLQERLNFALYHGKKFGDLVKNRKGPFAVISATDMNIGQKLTFTQETFDGLCLNLNDL
                     EISRAVAASSSVPLIFAPLTLNNNGGNCHFSMPQEFSAREKAIDGLITKNIEETNHIL
                     SLYQDSKERPFIHLVDGGLTDNLGLASVLEISDVMGIEEMYQYIHQLRVKNIVVINVN
                     AQNEVSSEIDKTANVPGVADVVNTIVNVPIDRTTQINLQRYRQFTDGWNEYANKQPSK
                     IEMHFVSLGLKELPDGDLKKDVLNIATSFYLPEEDVDKLREAARVLLQQSQEYNAALK
                     TLQ"
BASE COUNT         3070 a         2352 c         2045 g         3022 t
ORIGIN      
        1 tatttctaat ttatgaggat tctattatgt ctaaatgtcc tgttacacat ttgacaatga
       61 acaacggtgc acctgttgtt gataaccaaa acagcttaac agcgggtcca cgtggtccat
      121 tattagcgca agatttatgg ttaaatgaaa aattagcgaa cttcgtgcgt gaagtcattc
      181 ctgagcgtcg tatgcacgct aaaggttccg gtgcgttcgg tactttcact gtaactaacg
      241 acatcactaa atacactcgt gcggcaattt ttagccaagt cggtaaaaaa actgaaatgt
      301 ttgctcgttt caccaccgtt gcaggtgaac gtggtgcggc agatgctgag cgtgatattc
      361 gtggtttcgc attaaaattc tataccgaag aaggtaactg ggatatggtg ggtaacaaca
      421 cccctgtgtt cttcttacgt gacccacgta aattcccgga tttaaacaaa gcggttaaac
      481 gtgacccacg cacgaatatg cgttctgcaa ctaacaactg ggatttctgg acattattac
      541 cagaagcatt tcaccaagtg actattgtaa tgagtgaacg tggtattccg gcttcatatc
      601 gtcatatgca cggttatggc tcacatactt atagcttcat caatgcagct aatgagcgtt
      661 tctgggtgaa attccatttc catacacaac aaggcattaa aaacttaact aacgaagaag
      721 cagccgctat tatcgctaac gatcgtgaaa gtcaccaacg tgatttatac gaagcgattg
      781 agcgtggcga tttcccgaaa tggaaaatgt ttgttcaaat tatgcctgaa actgatgcag
      841 acaaagtacc ataccacccg ttcgatttaa ctaaggtatg gccgaaatca gactatccgt
      901 taattgaagt gggtgagttt gaattaaatc gtaaccctga aaacttcttt gctgatgtgg
      961 aacaatctgc attcgcacca agtaacttag tgccgggcat cagcgtgtct ccagaccgta
     1021 tgttacaagc tcgtttattc aactatgcgg atgctcaacg ctaccgttta ggtgtgaacc
     1081 accaccaaat ccctgtaaac gctccacgtt gcccggttca tagcaacgct cgtgacggtc
     1141 aaggtcgtgt ggacggtaac tacggtagta ccattcacta cgaaccaaac agcttcggca
     1201 aatggcaaga acaagctcaa tatgcagagc caccactgaa aattaatggt gatgcagccc
     1261 actggaacta tcgtgaagac gatgctgact acttcagcca accacgtgta ttgttcaata
     1321 tgatgaatga tgaacaaaaa caagcattat tcaataatac tgctgcaggt atgggcgatg
     1381 cattagactt cattaaatac cgtcatattc gtaactgcta tgcctgtgat ccggcatatg
     1441 gtgaaggtgt ggcaaaagca ttaggtatga cggttgaaga tgctattgct gcaagagcaa
     1501 cagacccagc attaggtcaa ccgggtttac tctaatcctt agtaaataca aaactcaaag
     1561 caaaagctct acaaaaaagt agagcttttt tattgctaat tacttaacca ccgtaatttc
     1621 ccacaaggca tcaccaagtt gttggtaatc ggtaacttca tagccttcct cttccgccca
     1681 atttggaatg gcttcggttg cttgagtaca gtcaaattca atcgttaagc tatcgccttt
     1741 attcaatttt gccatcgccg ctttggcttc gactaatgga aacgggcaaa caagccctga
     1801 ggcaggtaaa cgtacgttca tctttattac tccttaaaaa attaaccttt taaatgctgt
     1861 gggcggataa tgccaatgta attacactca ggataaacac taacacccaa ggggaaatac
     1921 caaatgtttc ataaatcgtg cggtgttgaa ttacattgct ttttagctct tgattaagtg
     1981 gggctaacac accggttcgc atagaagcag aaaacagccc ataaataacc agtgcaaccc
     2041 aactgccgat taaaccctca ccggcacgat accacgttcc tgtagcacaa ccgcctgcat
     2101 aaataatgcc gataccaaac agaaatgccc caatgatcac tgcgagcaaa ggcaaatctt
     2161 ccgccggaga actaaaagag ccggcttcat acaaaagcca aatcccgatc gattgcaccg
     2221 taattgcaat taaaagtgcc acaaacatac ggctattctt tgttacatag agatcacgaa
     2281 atgctccggt aatacaaaaa cgccctcttt gcatcacaaa cccgagtaga aagccacata
     2341 ataatcctgt gaataacata aaaaatacct ctcaacaaat taacgtgaga ggtatttaaa
     2401 tataaagccg tctagatggc taataatctt tagcaaaatg atatgttatt cagttataaa
     2461 tgctattaaa tatgtggaat tacatcatca ggtgcaccta atttattgag tgccactttt
     2521 cttggatcta ccaacttgga tttttttatc atcggttcaa ccgttgaacc ttgtactaaa
     2581 atcgagaaag tgactaccgc ataagtcatt aacaaaatca aatcacgtac atcaagattg
     2641 ccttctgagc tcgataccac cgcagcccct gccggtacgg aaagtgccat cgcaagggct
     2701 aatccaccac gtaaaccgcc ccaagtcata atttttaagg tgtacgggtt ataagtacgg
     2761 aaacgtttta acacttggta tggaatccac aggctgatat agcgtgcaag caaacaaatt
     2821 ggaattgctg ccaacattaa caccccacca ataaaggtga aatcaactaa cagtgtactt
     2881 aagccgataa gcaggaataa caggtagttt aatgaatggt cgatcatctc ccagaagtga
     2941 tcaagatatt tttggctctg ttcggagaaa ccggaacggc gagtccagtt gccaatcata
     3001 ataccggcaa ctaccattgt taatgcccct gaaacgtgta ggtagtttgc aatcacaaag
     3061 ccgactgttg gaatcgttag cgtaattaaa atttcaaggc ttccgtcatc ggttgcggaa
     3121 ataaatttat gtgccacaaa accggttaat aagccaaaaa cgataccgcc taccgcttct
     3181 tgcaagaaca gtccggtaat accgctaagg tttggttctt gtccgccaaa agcaacggca
     3241 aagatagtgg taaaaatcac caagccgata ccgtcattaa acagagattc gccctccacg
     3301 tgcatcgaaa ggcgttttgg ggctttcagg cttttgataa ttgccaatac ggcaatcggg
     3361 tcggttggcg aaatcagtga gccaaacagc aaacaataca caaagccgag ctctaaccca
     3421 agtagccttg ccacgccata aattaacgca ccgatcaaga aagtagaaac taaggttgaa
     3481 aataaggcat aaactgcaat ttcccgccgt tgctctttca ttaatggtag tttaatccca
     3541 agtgaaccgg caaaaagcaa gaaccccagc attccgttta acaggaagct tttaaaatcg
     3601 agttgttcaa aaatggaaat ggcaagttta tcaacattaa accagccgag agaaccgaag
     3661 gtaagaataa gaagggagcc gacaagggca gaggctgtaa ttgcaatagt ggtttgaata
     3721 ttttcgttta attttcgggt aaaaaacgaa ataaacatcg caagcccaaa cagcaagcag
     3781 atataagagt agagttgcat aacatttacc tataaatata aaaactgccg ctagtgtaaa
     3841 cgctgtttaa cagaatagct acataaaaaa taggcagaat acaatttgat aacattctgc
     3901 ctatttaggg ttatgcagtt gccaataatt tttctaaaat ttgataataa atttcgccac
     3961 aatgtgccaa atcatccgca ctcacacact cattcacttt gtgaatagtg gcgttcagtg
     4021 gtccaaattc cactacctct gcccccatta atgcaataaa acgaccgtcc gaagtaccgc
     4081 cacctgtgtc taaacgaggc gtaatttttg tgacattttc gaccgcttgt aaggcggctt
     4141 cgaccaattt tccattgccg gcaaggaatg gtttgcctga taaattccaa cttattcggt
     4201 atttcaaacc gtgtttttcg agcatttccg ctactttggc tttgatgatt tcatcggtca
     4261 cttcagtgca ataacgtaag ttaaactgca cataaagctc gcccggaatc acattattac
     4321 tgcctgtgcc ggctttgata ttggcaattt gcaaactggt aggcgggaaa aattcgttac
     4381 cgttatccca ctgataagtg gtcagctcgg ttaaaaaacc cagtgacgta tgcaccggat
     4441 tttccgccaa atgtggatag gcaacgtgcc cttgaatgcc ctcgatatag agatctgccg
     4501 taattgaacc acgtctgccg tttttaatca catcgccaaa caccttgcta ctagacggtt
     4561 cgcccactac cgcataatgt accgcttcat tgcgagccat taaggtttca accactttta
     4621 ccgtaccgtc tttggctgct gcctcttcat cggaggtaat caataatgcc actttgccct
     4681 tatggtttgg gtgatgttgc acgaaagttt ctgccgccac aactaacgcc gagagcgagc
     4741 ctttcatatc tgccgcccct cgcccataaa gcattccatc tacaatctca gccgaaaacg
     4801 gcggataagc ccattggctt tcatcgccta ccggcaccac atctgtatgc ccggcaaaca
     4861 caatgcaagg ctctgtttta ccattttccg agccgtgcgt tgcccataaa ttcaaggtgt
     4921 cgccaaacgg caaccattca atattaaagc ccagtttttc aagacgttgt gcaatttctt
     4981 gctgacagcc tttgtcttgt gggctgatgg aatcacggcg gattaggttc tgtgcaaggg
     5041 aaataatttc atttttcata ggttacaagc ggttgttttg ggtgaatttt ttgctatata
     5101 tcctgtaggg gcgggtcctg tgcccgcccg aacaatccta aagactacgg acaggcacag
     5161 gacctgtccc tacaagcagg ggaaaaaatt aattaaacgc agccacatac tctttctcat
     5221 taaaaccaat caaagcaata tcctctttaa tcacaatcgg gcgtttaatt agggtcggct
     5281 gttcggttag cacttttaat gccgactcac ggttaaggtt gtttttaatt tcctcatcaa
     5341 ggtttcgcca agtggtgcta cgtttattaa ctaagttttc ccaaccgaat tttgcttcca
     5401 tttccactaa ccaatcggca tttaaaccgt ccacacggta atcgtgcagt tgtgggtttt
     5461 gattatgatc ttcgagccat ttcaaggctt tttttactgt gtcgcaattt ttaatgccat
     5521 aaactaaggt tgtcatcatt tttcctcatt atctgattcc agcattgcct gccagatttt
     5581 taacgcatcg gcggtttctg ccacatcgtg cacccgtaaa attttcgccc cattttgagc
     5641 ggctaataac gcccctacta cagagccaag tagccgttgt tctaccggtt tatctaacac
     5701 cgcaccaatc attgatttac gggaaagtcc ggcaagcaca gggaagcctg aatccacaaa
     5761 ggctttaagg tgtttcaata attggtaatt gtgtttcacg cttttaccaa agccaaaacc
     5821 ggggtctaaa ataatgtgtt ctttgggaat gcctgcggtg atgcactcga aaatccgttg
     5881 atttaagaaa tctgccacat cttccaacac atcatcatag tgcggatttt gttgcattgt
     5941 ttgcgggttg ccttgcatat gcattaaaca aaccggcaag cccaattcta ctgccgctgc
     6001 caacgcattc ggctcggtta aggctcgaat atcgttaatg atatccatac cgacttttgc
     6061 cgactctttg aatacttctg ctttggagct atcaaccgaa atcaagcaat caaaacggct
     6121 gcggatcgcc tccaccaccg gcacaacacg ctctaattct tgttctaagc tcacaatcgc
     6181 cgcattaggg cgggtggatt caccgccaat atcaataatg cttgccccat cagctaacat
     6241 tttttccgct tggaacaagg ctttatcgag ggaaaaaaac ttgcccgaat cggaaaatga
     6301 atccggcgtg aagtttaaga tccccataat ttgcggtgtg gttaaatcta acaagcggtc
     6361 atttttttga aaatttttga aatggatttg catatttttg cttatatttt gttggtgcaa
     6421 gcgtccacgc ttgtaccact ctatgttgat ttacagcaca agcgtggacg cttgcgctat
     6481 cttactctct cattaagtta tttagtaatt agcccttaat tttgccaata cgccttttaa
     6541 agttttatcc agcggggcgg taatgaccat ttcttcgccg gttttggggt gttcaaaacg
     6601 aatagcatgg gcgtgcagaa ataaacgctg taagcccact tctttcattt tgctgtcaaa
     6661 ttccgcatca ccatatttgt catctaacgc aatcgggtgt ccggcatatt gggtatgcac
     6721 acggatttgg tgggttcttc cggtaatagg cgaagctttc accaaagtgg cattggcata
     6781 acgctcttca atgctgaaac gggtttcgga cggtttaccc tcttcactca cccgtacaat
     6841 tcgctcaccg ctggcaagtt cgttttttag caatggagct ttaatcactt tggtatgagc
     6901 ctgccattgc cctcgcacaa gggcaagata gtctttttgt actacttttt cacgcagttg
     6961 ttcgtgcaag gaacgtaacg ccgaacgctt tttcgccacc agcaaaatgc ctgatgtatc
     7021 acggtctata cggtgtacca attccaagaa acgagcctgt ggacgtaatg cacgcaaggc
     7081 ttcgatcaca ccaaaactta aaccactgcc accgtgtact gcaatgcctg aaggtttgtt
     7141 aattactagc attgactcat cttcatacag aatttggctc tccagctccg ccactttgtt
     7201 taatttagtc gaaattgggg cttcattttt ttccgccaca cgcaccggcg gcacacgcac
     7261 taaatcatca ggctgtaatt tatattcggg tttgtttcgt cctttattca cccgcacctc
     7321 gccttttcgc acaatgcggt aaatcaaact tttcggcacg cctttgagct ttgccaataa
     7381 aaaattatct aaacgctgtc cggcttcatc ttcactgatc gtaaaaaatt gaacgccggc
     7441 actaattact ttttctgttg tcattatctt gtcttttaga gaaaattttg ctctaaattc
     7501 tagcacaaaa ccgcttagcg agtgaggcaa attcgctagg cttatttttc attttaattt
     7561 taaggaaaca atatgactga aaaagtcacc gaaacagctc caaacattga cgtggttgcc
     7621 gaaaccgaaa aagtaatcca caaagtcagt aatatgaata ttgaaactat gctaaatgag
     7681 tggattatcc cttatggcac caaaatttta ctggcgattg cgatttttgt agtcgggaaa
     7741 tttcttgccc gcctaattag ccgtttactc ggtaaagccg cattaaaatc aaccaaagat
     7801 gaaatgctgc aaagttttat tgtctcaatc agctattttt tactgctgtt aattgtggtg
     7861 attgcctcgc tttcacaaat cggcattaac accagctcgc tggtcgcatt aatcggtgct
     7921 gccggtttag ccatcggttt atcattacaa aactcattac aaaactttgc tgccggcgtg
     7981 atgttgttaa tcttcaagcc atttcgcaaa ggggatttaa tcgaaaccgg cggaatgacc
     8041 ggcgtggttg aacaaatggg gcttttagtg ttggaacttc gcaccggcga taacaaaacc
     8101 gtgttattac caaacggcaa agtgttttct gacagcatca tcaactactc taacaacccg
     8161 acacgccgta ttgatttcac ctttgatatt tcttatgaat ctaacttaaa agaggcgaaa
     8221 gagattatcc aacgcatttt agacgccaat acttatgtat taaaagatcc ggcacctgta
     8281 gtagcagtcg gcgctttagc accgcatagt gttcaacttg ttgttcgccc ttgggttcag
     8341 acaccaaatt actgggcagc atattgggaa attatcgaac aagtaaaatt agcctttggt
     8401 gaagcaggta tatctattcc ttacaaccaa atggaattgc atattaaaag cggttcacta
     8461 gaaaataaag cataagaaaa aagcctgtaa gcggtctttt ttcgataaaa ttttgcaaat
     8521 tttgggcgaa ggtttatact ttagctaaat aatctacgaa caggagcata ttatgcgtat
     8581 tgatacctct gcactttgcg atctttattc tgaccaagtt gatgtggttg aaccaatttt
     8641 ctcaagcttt ggcggtgctt cttccttctt tgggaagatt accacggtta aatgctttga
     8701 aagcaacggt ttaattgctg aagttttgga agaagagggt gaagggcgag tgctgttagt
     8761 cgatggtggc ggtgcggttc gtcgtgcctt aattgacgca gagctggctc aacttgctgt
     8821 agataacggc tgggaaggta tcatcgttta tggtgcggtt cgccaattag atattttgga
     8881 aagattagat atcggtattc atgctttagc gccaattcca gtgggagcag ataataccga
     8941 cattggcgaa gtggatacgc ctgtgaattt tggcggtgta accttcttcc ccgaagatta
     9001 cgtttatgct gacttaaccg gcattattct ttcgcaagag ttgttggatt taaacgacat
     9061 cagcgaataa atatttgcaa aaaaatacca aaaaccgacc gcttggtcgg ttttttacta
     9121 ctgcagtgtt tttaatgccg cattatattc ttgagattgc tgcaataaaa cccgagcagc
     9181 ttcacgtaat ttatctacat cctcttccgg caaataaaat gatgttgcaa tatttagcac
     9241 atcttttttt aaatcaccat caggtaattc ttttaaacct aaactgacaa aatgcatttc
     9301 gatctttgat ggctgtttat ttgcatattc attccaacca tctgtaaact ggcgataacg
     9361 ttgtaaatta atttgtgtcg ttctatcaat tggcacatta actattgtat tcactacatc
     9421 agccaccccc ggtacatttg cggtcttatc gatctcgcta gatacttcat tttgcgcatt
     9481 tacattaatc actacaatat ttttcaccct aagctgatga atatactggt acatctcttc
     9541 aatacccata acatcagata tttccagcac actggcaagc cctaaattat ctgttaatcc
     9601 accatcaact aaatggataa aggggcgttc tttactatct tgataaaggc tgagtatatg
     9661 attcgtttct tcaatatttt ttgtaattag tccatcaatt gctttttctc tagcactaaa
     9721 ttcttgtggc atactaaaat ggcaatttcc accattgtta ttcagtgtaa gcggcgcaaa
     9781 aatcagagga acagaactag aagcagcaac agcacgggaa atttctaaat cattcaaatt
     9841 taaacaaagc ccatcaaagg tttcttgcgt aaaggtcagt ttttgaccaa tgttcatgtc
     9901 tgttgcgcta atcacagcaa aaggtccttt acgatttttc actaaatcac caaacttttt
     9961 accatgataa agggcaaaat ttaagcgttc ttgcaataaa tcaccacgcc catattgagg
    10021 agaacttaaa cgaggaagat tggataacga aaacacctct gtaataatat ctttttgaaa
    10081 gttcgctttt aaaaaacggt cagcaaattt aggaatgata tcctttcctt ccaacgcaaa
    10141 ataagtggct aatatcgatc cacctgatac gccaaaggca atatctatat tctctaataa
    10201 agtattacct ttatttgttt ctttcaccct taccttttta aactcttcca acactccata
    10261 accaagcgcc gccgctcttg ttccaccacc tgaaaacatg atgacaacaa tattaccgtc
    10321 ttttgcactt tcagcgatca catttttttg tcgataacct ctttcaacat ttacctgttt
    10381 aattgtttca actggcttat aaacaaccaa actgcaagca gataacacta gcgtaaaggc
    10441 gaagatgaaa agctgtttta ttttgtttag aaaaagcata aaaacccta
//
LOCUS       DANLRK010000090        10222 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.090, whole genome shotgun sequence.
ACCESSION   DANLRK010000090 DANLRK010000000
VERSION     DANLRK010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 10222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10222
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.090"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(67..561)
                     /locus_tag="PXA33_001622"
     CDS             complement(67..561)
                     /locus_tag="PXA33_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL5120941.1"
                     /translation="MLSGLKIESFSKEFKRDCFDSESELLNQYLKTQVSQDIKRRITS
                     CFLAVNSEQTVVGYYTLASTSLSLSDLPITLQRKLPRYPTVPAVLLGRLAVDKRVKGL
                     GLGQALLGNALKRVLRADIAAYALIVEAKDESAVNFYRKFGFIDFENEARKLFFPLDR
                     VRLS"
     gene            complement(561..833)
                     /locus_tag="PXA33_001623"
     CDS             complement(561..833)
                     /locus_tag="PXA33_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HDL5120942.1"
                     /translation="MATATARFEARMSQEVHMLLKRAASLEGRSLSDFVVGAALSAAR
                     KTVEQNDIIRLSVNDQELFAKALIDPPEPNLAMQKALALSSQILGE"
     gene            complement(992..1420)
                     /locus_tag="PXA33_001624"
     CDS             complement(992..1420)
                     /locus_tag="PXA33_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5120943.1"
                     /translation="MRKLLVITALTLCTTPVFAADKNVIFSCTSTEGKPLTVKRVGND
                     YEYSYDKTTFKNPIKKAVTNDGSIIARGSGFTTYALELENDGLKYLVGFVQPNGNAKE
                     FIEPGATISQSKEQPSIGSVDCDTRKKSHYKFDVHLMNTL"
     gene            complement(1441..3447)
                     /locus_tag="PXA33_001625"
     CDS             complement(1441..3447)
                     /locus_tag="PXA33_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HDL5120944.1"
                     /translation="MKSQFKFKKTHLLVITVLFSSTSSISFADSITDKLEFSKSDSII
                     SDRVFYQIGGGTGYMAPPSRGNTKVVDLGIGWKANLTCGNFDIKTTIKNDLNGLKDGF
                     KDLMGNVIDSAKGAVASMPAMILQRANPQLYDLLTNGMYQGKLDFNRLKTSCEEMSKV
                     LADHTLGGKWGQAADLESYKQITSTQPDAKQAQKDLEDKKGKDGRVWVGGQRKGGQNQ
                     DPINVVGDVAKGGFNLLNKRNVLDSSPVSNCEGNLCQTWDTPQAAADFLTKIIGEQSI
                     STCKEDCGTKTSSKAGVGLSPEIEEEHIRTIVKLENVLNMDVPKSELLAELSSSSVPV
                     TRGLIESLRDDPDVELLSQRLSAEIATSKVMEKMLLARRAILAGMREPNVAQNADAQV
                     ELERVLNIIDREISQVKLEMDMQKMITGNTASVILQSKSEREALAGAHSLSQDSLDKR
                     VQGLENGNVALTDLETGSNINLSRRNIALSIPNIPNVAALIPTLPNRGGSGVGGNFPS
                     LAQISGSTMDQATGLLRNFEGFISKAQWDVNAHRVGYGSDTITRADGTVVKVTKDTVI
                     TKEDAERDLARRTAIFANNVRQELGDSHWNALPANAQAVLVSYAYNYGSLAKTKSVLD
                     AARRSAQTGDMTALATAVRNRQVDNNGINARRRNQEADYILGKN"
     gene            complement(3462..4403)
                     /locus_tag="PXA33_001626"
     CDS             complement(3462..4403)
                     /locus_tag="PXA33_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03756 family integrating conjugative element
                     protein"
                     /protein_id="HDL5120945.1"
                     /translation="MKRAFFSVLLSVSLAVPTTLYPQQVKASLNTASIAASSASTSCL
                     DYKVVGTCFWLFCTYFGCKVRTSTKIKHYLPEMVVSSYNQDGQTPWTELQSLNPGVKG
                     GEYRSPQRQYSQLTFKNADAFGHPQGAIGQLLGSTGYYCKSQTTPFIPYFLSGLDFFA
                     WRENMPEMFYPEALIPGMREVGRVGDSWGNIYPRSGWVTQAHDYKASATIVQRVADIV
                     TRTAQPHIYFPAAKKGGVGEWPPGPVEEGQARTHKWQMLYPQMSNTCSVFPNGGQTDT
                     YSSQISNQGDYAWALWRPYSCCKRRGQTFLYSIDWSH"
     gene            complement(4400..4843)
                     /locus_tag="PXA33_001627"
     CDS             complement(4400..4843)
                     /locus_tag="PXA33_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03757 family integrating conjugative element
                     protein"
                     /protein_id="HDL5120946.1"
                     /translation="MKASRFLGYFSIVTVVFSVVAQAQPNIIVYTTKMYPIQNSQLAS
                     QVYYLDQVENFEEEISRNLSKSPEIAEREIRAFFNSADWKAQEEQLKDAYQGVISGWQ
                     NGIKKVPAVFFNNLQSSPSVIYGMTDIQKALDEWHKWVQQHGESK"
     gene            5218..5571
                     /locus_tag="PXA33_001628"
     CDS             5218..5571
                     /locus_tag="PXA33_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5120947.1"
                     /translation="MSQQTAGWDVIPFESSKTAKPSLFWKLGNKLGNIFVSAKHKAKS
                     KYVEVTKGINQRIAEEDEMRALELQMCLDEQAAEYEQEISRIKRKMFFRALFVAIIAF
                     LCGMATVFGTQLSLI"
     gene            5643..5888
                     /locus_tag="PXA33_001629"
     CDS             5643..5888
                     /locus_tag="PXA33_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemophilus-specific protein"
                     /protein_id="HDL5120948.1"
                     /translation="MSVHIKLSCVGDILPIKLNEWTPEHRALHHEISHKALEAWQNRD
                     KGTQSVRFMQQMPARQGLHFRFLSVSEQDGKLIVSRS"
     gene            5897..6139
                     /locus_tag="PXA33_001630"
     CDS             5897..6139
                     /locus_tag="PXA33_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5120949.1"
                     /translation="MFGKLFYKMNPKVLAKIGEDFRKAGYLMGAGFVGMVISNDQIST
                     SEGFVLFLWGLYIWIIGHIALHHSEKILNSKQGEPK"
     gene            6403..7245
                     /locus_tag="PXA33_001631"
     CDS             6403..7245
                     /locus_tag="PXA33_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5120950.1"
                     /translation="MSDSVLNTYFKHALVKAGFPDTLDMEYSLSHCQGDGVAFYGRLG
                     TDDLIQLFNHLNPQKRKQKMFANLLTHIESWETHDDIGFEIIRNRFGYRYSHWNTMEL
                     YAKTADQLVFFNDSDARRNWYFPTSKVDKYKALWDGFIEDLEQYIKDISREMESVGYQ
                     IIEASPYQTETVFKFNTEKYLVELQRKPSEFYSELVDWAFGDMCDFEAAIEQICQGQL
                     CYADFEATVTDKKSEIQLGLARLSGISYSPNDRSFSGYRQELIGEAIASARHNAARFA
                     YLHA"
     gene            7360..7773
                     /locus_tag="PXA33_001632"
     CDS             7360..7773
                     /locus_tag="PXA33_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemophilus-specific protein"
                     /protein_id="HDL5120951.1"
                     /translation="MKNEHNLEQLKYIWDNRNLENKVYQYLFHICIEPMKKGDWNRYT
                     IWLNFHGNLINVSDVVYRVVESQKLKDDIKIWYDKQYYCRPTKVETTGFISIFLHLFY
                     EGIARILLEQGMITETEAAEKSYNFRAANCFQRLL"
     gene            7787..7942
                     /locus_tag="PXA33_001633"
     CDS             7787..7942
                     /locus_tag="PXA33_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5120952.1"
                     /translation="MEPRTVKVIYDDGDYFFTLINADEQTIRNYYAIGSSIGFDNTRK
                     IVAVEIL"
     gene            8022..9005
                     /locus_tag="PXA33_001634"
     CDS             8022..9005
                     /locus_tag="PXA33_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1738 domain-containing protein"
                     /protein_id="HDL5120953.1"
                     /translation="MNPQSNTQPKFDLYQEVTDKIIAALEQGTAPWLKPWDSVIPYGG
                     MPCNAISGRLYSGINVLLLWLSAAEHGYIQRKWITAKAAIEAGGHVRKGEKATLAVSY
                     RPYDREKTDDDGNLVLDDDGNPVIEHLASIKKHCLFNIEQCEKLPQDWYETVETPEQL
                     TDENQYAIFEEIRLMLKGMDLQVMVKPSNQAYYHPRNDHIVMPEMKQFHSPQKFHSVL
                     LHEMTHATGHQSRLARDGIVSGKARFGNKVYAFEELVAEMGCAFLCSHLGFADVPQNA
                     SYIESWIKVLREDKRAIFRASGKAREACQYMLDALNIAQLADKYEVFEEAA"
     gene            9810..>10222
                     /locus_tag="PXA33_001635"
     CDS             9810..>10222
                     /locus_tag="PXA33_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraI domain-containing protein"
                     /protein_id="HDL5120954.1"
                     /translation="MHSILKKILGKSKSSSQSSVKSDNVFAIEQDSEGWLYPKNASQL
                     LNTELRQKYLGLLWQQVSMNRQMFEALYQQPIERYAEMVQLLPASESHHHSHIGGMLD
                     HGLEVIAFSAKVRQNYILPPNVAPEEQSKQRDAWTA"
BASE COUNT         2978 a         2080 c         1993 g         3171 t
ORIGIN      
        1 ttctttaaaa gctcgaattt tcctgcctaa ccaataacca aaattatttt tcataatcac
       61 tctccattaa gataatctca ccctatctag tggaaaaaat aatttcctag cctcattttc
      121 aaagtcaata aatccaaatt tacgataaaa attgaccgca ctttcatctt ttgcctcaac
      181 aattaatgcg taagctgcta tatctgcacg aagtaccctt ttaagtgcat tacctaatag
      241 agcttgccct aaccctaatc cctttacacg cttatctact gctaatcgac caagtaggac
      301 ggcaggtacc gtaggatatc ttggtaattt cctctgtaat gtaattggta aatcagataa
      361 tgaaagactt gttgaagcca gtgtgtaata tcctactact gtctgttcag aattaacagc
      421 taaaaaacaa gaagtaatac gtcttttaat atcttgtgaa acctgagttt tgagatattg
      481 atttaataat tcagattcac tatcaaaaca atctcgttta aattccttgc taaatgactc
      541 tattttcaat cccgaaagca ttattccccc agaatctgtg agcttaatgc caaggctttt
      601 tgcattgcca aattaggttc aggaggatca atcaatgctt tagcaaaaag ttcttggtca
      661 ttcacagata atcgaataat atcattttgc tcaactgttt ttcttgctgc agatagtgca
      721 gctccaacaa caaaatcact aagactacgc ccttcaagag aagcggctcg ttttaacagc
      781 atatgaactt cttggctcat tctagcttca aatcgtgcgg ttgcagttgc catatatttt
      841 ctccttgaat tatttagttt taattgtacg tatttatacc gtacaaatca agcatgacaa
      901 taaaaaaatc ccttagattt tcaaacctaa gggatttacc taccgatctt tgtgtttttt
      961 aacgtgggct ggccctagtg ccatacaggc attataaagt attcatcaaa tgaacatcaa
     1021 acttataatg gcttttttta cgagtatcac aatcaacaga gccgatactt ggttgttctt
     1081 tactttggct aatcgttgct cccggctcaa taaattcttt tgcattgcca tttggctgaa
     1141 caaagccgac caaatacttc aatccatcat tttccagctc taatgcatac gttgtaaaac
     1201 ctgaacctct ggcgataatt gagccgtcat tagtgactgc ctttttaatc ggatttttga
     1261 aggttgtttt gtcatacgaa tattcgtaat catttcctac ccgtttaaca gtaagcggtt
     1321 ttccctccgt gctggtacag gaaaatatta cgtttttatc tgctgcaaat actggggtcg
     1381 tgcagagtgt aagagcagta attactaata atttacgcat aaatgtaact ccttataagg
     1441 ttaattttta ccaagaatat aatcagcttc ctgattccgg cggcgtgcat tgataccgtt
     1501 attatcaacc tggcgatttc tgacagcagt tgccaaagca gtcatatcac cagtttgagc
     1561 tgatcgtcta gctgcgtcaa gcacactttt tgttttcgct agtgagccgt aattatatgc
     1621 ataagacacc aaaacagcct gagcgttcgc cggtaatgca ttccaatgtg aatcacctaa
     1681 ctcttgtctc acattgttcg caaagatagc agttctgcga gctaaatcac gttcagcatc
     1741 ttctttggta atcacagtat ccttggtcac tttaactacg gtaccgtctg cccgtgtaat
     1801 cgtatcggaa ccatatccta cacgatgggc attcacatcc cattgagcct tggagataaa
     1861 gccttcaaaa ttacgcagta accctgttgc ctgatccatc gttgaaccgc tgatttgtgc
     1921 aagtgatggg aaatttccgc ctacacctga accacctcta tttggtagag tcgggatgag
     1981 agctgctaca ttcggaatat ttgggatact caatgcaata tttctacgag agaggttaat
     2041 attagagcct gtctctaaat cagttaatgc aacattaccg ttttcaagtc cttgaacacg
     2101 cttatccaga ctatcttggg ataaactatg agcaccagca agagcttccc gttcggattt
     2161 actttgcaga atcaccgaag ccgtattgcc ggtgatcatt ttttgcatat ccatttccag
     2221 ttttacttgg gaaatttcac ggtcaataat attgagtact ctttccagtt ctacttgagc
     2281 atccgcattt tgagcaacat tcggctctcg cataccagct aggattgctc ggcgagccag
     2341 tagcattttt tccattactt tactggtcgc aatttcagcg gataaacgtt ggctgagaag
     2401 ttcaacatct ggatcatcac ggaggctttc aatcaagcca cgagtgacag gtacactact
     2461 tgagcttaat tcagctaata gctcggattt cggcacatcc atattcagta cattttctaa
     2521 tttaacaatt gtacgaatat gttcttcttc aatttccgga cttaaaccaa caccggcttt
     2581 cgaactggtt ttagtaccac aatcctcctt acaggttgaa attgattgct caccaataat
     2641 cttagttaag aagtcggcag cagcttgagg tgtatcccaa gtctgacaaa gattaccttc
     2701 acaatttgaa accgggctgc tatctaagac attccgctta ttgagtaaat taaatccgcc
     2761 tttcgctaca tcaccaacga cattaattgg atcttggttt tgcccacctt tacgttgccc
     2821 accaacccaa actctcccat ctttcccttt tttatcttct aggtcttttt gggcttgctt
     2881 cgcatcaggt tgtgtcgagg taatttgctt atagctctct aaatctgctg cttgccccca
     2941 ttttcctcct agtgtgtgat ctgccagaac tttagacatt tcctcacagg aagtttttag
     3001 acggttgaaa tccagcttac cttggtacat accatttgtc agcaaatcgt agagttgagg
     3061 gttggcccgt tgcaatatca ttgcaggcat actggcaacc gcacctttag ctgaatcaat
     3121 gacattcccc attaaatctt taaagccatc tttaaggccg tttaaatcat ttttaatagt
     3181 tgttttaatg tcaaaattgc cacaggttaa attcgctttc caaccgatgc ctaaatcaac
     3241 gactttggta ttaccacgac taggtggagc catataacct gttcctccgc cgatttggta
     3301 aaagacacga tctgaaataa tcgaatccga cttactaaat tccagtttat cagtgattga
     3361 gtcagcaaaa gagatagagg aagtactgga aaaaagtact gttattacta atagatgtgt
     3421 tttcttgaat ttaaattgtg atttcattgc aagacctcgt tttagtgtga ccaatcaata
     3481 ctgtaaagga atgtttgccc tcggcgttta caacaactat aaggtcgcca tagtgcccaa
     3541 gcataatcac cttgatttga aatctgagag gaataggtgt ctgtttgccc cccatttgga
     3601 aaaacagagc aggtatttga catttgagga taaagcattt gccatttatg tgttctggct
     3661 tgtccttctt cgacaggtcc tggtggccat tctccaacac cacctttttt cgctgccggg
     3721 aaataaatat gtggttgagc agttcgggtc acgatgtctg caactctctg cacaatggtt
     3781 gcagaggctt tataatcgtg agcttgtgtt acccaaccgg aacgtgggta aatatttccc
     3841 cagctatcgc ctacacggcc aacttcccgc attcccggaa tcagagcttc cggataaaac
     3901 atttccggca tattttcacg ccaagcaaag aaatctaatc cacttagaaa atagggtata
     3961 aatggtgtgg tttgactttt acaatagtag cctgtacttc ctaataactg accaattgct
     4021 ccttggggat gcccgaaagc atctgcattt ttgaaggtta gttgagagta ttgacgttgc
     4081 ggagaacgat attctccgcc ttttacgcca ggattgagag attgtaattc tgtccacgga
     4141 gtttgcccat cctggttata agatgaaacg accatttccg gtaaataatg cttaattttg
     4201 gttgatgtac gaaccttgca tccaaagtag gtgcaaaata accagaagca cgttccgacg
     4261 actttatagt ctaaacagct tgttgaggca cttgatgcag caatcgacgc cgtattcaat
     4321 gatgctttta cctgttgagg atataaggtt gttggtactg ctaaacttac agagagaagt
     4381 accgaaaaaa acgctctttt cattttgatt caccgtgttg ttgaacccat ttatgccatt
     4441 catctagtgc tttttgtata tcagtcatac cataaatcac gctcggagag gattgcagat
     4501 tattaaaaaa caccgcaggc acttttttaa ttccattttg ccatccactg atcacccctt
     4561 gataagcatc ttttaattgc tcttcttggg ctttccaatc tgctgaatta aagaatgctc
     4621 gtatttctcg ttcggcaatc tcgggggatt tactgagatt tcgactgatt tcctcttcaa
     4681 aattctctac ttgatcaaga taataaactt gggaggcaag ttgtgaattt tgaatggggt
     4741 acatctttgt tgtatagaca atgatattcg gctgtgcttg tgccactaca gaaaacacta
     4801 ctgttactat gctgaaatat cctagaaatc tacttgcttt catcactctc tttccgaata
     4861 aataagcaga aaattagcga gatttttaca tagaggatag gaaaaactct tttcaaaaaa
     4921 gagatatttt tttgagcaaa aaatggttga ttttataaat ggatttcgta tgatatggaa
     4981 tattaagatg ctgtggaaac agcccttaaa agtcgcataa ctcttgagaa gcggcggcta
     5041 tccatcgagg tagttgagtt atttacccaa aatgtctccc agcattctga aagggaacgc
     5101 tgcaaatttg caggaatgcc acctaaacac ctaccttccg agggatattt caattccctt
     5161 ggaattgcga tctcctgagg aattttattt atttaacttt ttataggaga actcgctatg
     5221 agtcaacaaa ccgcaggatg ggatgtgatc ccatttgaat cgtcaaaaac cgcaaaacct
     5281 tctctttttt ggaaacttgg caataagctc ggtaatattt tcgtttcggc gaaacataaa
     5341 gctaaaagca aatatgtcga ggtaaccaaa ggcatcaatc aacgaattgc tgaagaagat
     5401 gaaatgcgag ccttagaatt gcaaatgtgc ttagatgagc aagcagctga atatgagcag
     5461 gaaatttctc gcattaagcg taagatgttt ttcagggctt tattcgttgc gattattgcc
     5521 tttttatgtg gtatggctac cgtttttggt actcagctct ctttaatcta atttaactca
     5581 cccatcgggg aaatgctatt tcccttgtgg taatacattt ccccttaatt ttggagaaat
     5641 gtatgtcagt tcatatcaaa ctctcctgtg ttggagatat tttgccaatc aaactcaatg
     5701 aatggacacc ggaacatcga gccttacatc acgaaattag ccataaagct ctcgaagcct
     5761 ggcaaaatcg agataaaggc actcaaagcg tgcgatttat gcaacaaatg ccagcccgac
     5821 aaggactgca tttccgcttt ctgagtgtat cggagcaaga cggcaagctc attgtcagtc
     5881 gttcatagtt ggttcaatgt ttggtaagct tttttacaaa atgaatccca aagtactagc
     5941 taaaatcgga gaagatttta gaaaagctgg ttatcttatg ggagctggtt ttgtcggaat
     6001 ggtaatttcc aatgatcaga tttccacatc ggaaggattt gttctttttt tgtggggatt
     6061 gtatatttgg attatcgggc atatcgcact acatcattct gagaaaatac ttaattcaaa
     6121 gcaaggagaa ccgaaatgag tccgtacttt ttaggtgctg ctattggaac agcgttattg
     6181 gcaacaattt ttattgttct agtaacttgg gcaaatcgag atattttgtt tacaaaatca
     6241 gataagccac agtctaaata gcaaaactcc attctaaccc ataggggaac aagttccccg
     6301 aggagcttgt tctccgtttt accttccttt taccctatcg ggaaatactt tcccgaattg
     6361 ggcttaagtg tttcccatta ttttttattt tggagaaaca ttatgtcaga ttcagtatta
     6421 aacacttatt ttaaacacgc tcttgtcaaa gctggttttc cggatacctt agatatggag
     6481 tattcactca gccattgtca aggagatgga gtcgcatttt atggtcggct tggtacagat
     6541 gatctaatcc agcttttcaa tcatctcaat ccacagaaac gtaaacagaa aatgtttgct
     6601 aatttgctaa cgcacattga atcttgggaa acccacgatg atattggttt tgaaattata
     6661 cgaaatcgct ttgggtatcg ttactcgcat tggaatacga tggagcttta tgctaaaact
     6721 gcagatcagt tagtgttttt caatgatagt gatgctcgaa gaaactggta ttttcctact
     6781 tcaaaagtgg ataaatacaa agcattgtgg gacggattta tcgaagattt ggagcagtac
     6841 atcaaagata tttcccgtga aatggaatcg gtcggttatc aaattattga agcctcgccg
     6901 tatcaaacgg aaaccgtttt caaatttaat acggaaaaat atctagtaga actgcaacgt
     6961 aaaccttctg agttttattc agagcttgtt gattgggcat ttggtgatat gtgtgatttt
     7021 gaagcagcta tagagcagat ttgtcaaggg caactctgtt atgctgattt tgaagcaact
     7081 gttaccgata agaaaagtga aattcagtta ggtcttgctc gcttgagtgg aataagttac
     7141 tcgccgaatg accgttcgtt tagtggttat cgacaagagc ttattggtga ggcgattgcc
     7201 tctgctcgcc ataatgcagc acgttttgcc tacttgcacg cctaatttgc agatttttac
     7261 tttaatttga ccgcttgtag tggttaattt tcaccccgtg gggaaatatt ttccctattg
     7321 ggcaaaatgt ttctccactt tttaatttgg agaaatgtta tgaaaaatga acacaattta
     7381 gagcaactta aatatatttg ggataaccgc aaccttgaaa ataaagtcta tcaatatctg
     7441 tttcacatct gcattgagcc gatgaaaaaa ggagattgga atcgttatac gatttggctt
     7501 aattttcacg gtaacttaat caacgtgtca gacgttgtat atcgagttgt ggaatctcaa
     7561 aagctcaaag atgacatcaa aatttggtat gacaagcaat actattgccg tccaaccaaa
     7621 gttgaaacaa caggttttat ttccattttt ctgcacctgt tttatgaggg catagctcgt
     7681 attttgctgg aacagggaat gattactgaa actgaagcag ccgagaaaag ttacaacttc
     7741 agagctgcaa attgttttca acgtttgctt taatggaggt gagataatgg aacctcgcac
     7801 tgttaaagtt atttacgatg atggcgatta tttctttacc ctaatcaatg ccgatgaaca
     7861 gactattcgt aattactacg ctataggcag ttcaatcggt tttgataaca caaggaaaat
     7921 tgtcgctgtt gaaattttat aaactaccca tcggggaaac gacttcccca tagggcgata
     7981 gtgtttcccc atttttctac ccgaactttg gagaaacact tatgaaccct caatcaaaca
     8041 cacagcctaa atttgattta tatcaagaag tgaccgataa aatcatcgcc gctttagagc
     8101 aaggaacggc tccttggctt aaaccttggg atagcgttat tccttatggt ggtatgccgt
     8161 gtaatgccat atccggaaga ttgtattccg gaatcaatgt tttgttactt tggttgtctg
     8221 ccgcagaaca cggctatatc caacgcaagt ggatcactgc aaaagcagct attgaagccg
     8281 gtggacacgt tcggaaaggt gaaaaagcaa cccttgcggt cagctatcgc ccgtatgatc
     8341 gagaaaaaac cgatgatgat ggcaaccttg tcctcgatga cgatggtaac ccggtaatcg
     8401 aacatctcgc ttcgattaaa aagcactgcc tattcaatat tgaacagtgc gaaaaactgc
     8461 cacaggactg gtatgaaacg gttgaaaccc cagaacagct gactgacgaa aatcagtatg
     8521 ctatttttga ggagattcgt ttgatgttga aaggtatgga tttgcaagtg atggtaaaac
     8581 caagcaatca agcttactac cacccacgca atgaccatat cgtgatgccg gaaatgaagc
     8641 agtttcattc accacaaaaa ttccatagcg tgctgctaca tgaaatgact cacgcaacag
     8701 ggcatcaatc tcgccttgct cgtgatggta ttgtttccgg aaaagctcgc tttggcaata
     8761 aagtttatgc ttttgaagaa ttggtcgctg aaatgggctg tgcattcttg tgttctcacc
     8821 taggctttgc tgacgtgcca caaaatgcca gctacattga aagttggatc aaggtgttaa
     8881 gagaggataa acgggcaatt ttcagggctt cagggaaagc tcgggaagcc tgtcaatata
     8941 tgctggatgc cttaaacatc gcacagctgg ctgataaata cgaagtattt gaagaagccg
     9001 cttaataaat ttgccgaaag gcatttcccg tggggagata ctctcccttc ggggcattgt
     9061 gtctcccttt ttattcactt aacgagagga ctcaatgctc aatctaaact tatttaaacg
     9121 acccattgaa atttcgacac ttgaatcttg gtcaaaattc tgtgatgatg ttggtaaagt
     9181 agcttttgtt gcagtacctg ctatgctgta tagtaattac gagttttgga ttaagataag
     9241 cagtataatt ggtctattta tagctaccta tttctgttta gttatcggta gaaatatacg
     9301 ccaatttatt gagaaggaga aatcaaaatg ggattaatga tcggcatttg gattattgct
     9361 tttattgcat ttgggttggc catatggaca agccatattc taaaaaagca agatgaacgc
     9421 cttaaacagc taaaaaatag ctaactttca aaatacggca cattttgtgc cgtatttctt
     9481 ttgtactggt ggcaaatata agattactat catcagtata ttgtaatatc tcaactcaac
     9541 agcattagtc aaaaatcctc caacatattc agagagtttt tcctactgag ttctcttatt
     9601 ttttggttca atactctccc tttataaatt aaatttggtt cagataaaag ccgctcaatg
     9661 gcaaaaggat aaaggagccc ttacgggcaa gggtaatccc ctaaggggga ataagggtaa
     9721 cctttccaag gggaaaggtc ttacccaaaa gggcaaaggg ctaaacggct ttctttcaac
     9781 gcaaacatca tctttttcgg tctatttcaa tgcactcaat tctcaaaaaa attcttggca
     9841 aaagtaaatc ctcgtctcaa agttcggtta aatctgacaa tgttttcgct attgagcagg
     9901 atagtgaggg ctggttatat ccgaaaaatg cctcccaact acttaatacg gaattacgcc
     9961 aaaaatatct gggtctactt tggcaacagg tatcaatgaa ccgtcaaatg tttgaggcac
    10021 tttatcaaca gccaatagag cgttatgccg aaatggtgca actgttaccc gcttccgagt
    10081 cacatcatca ttcacatatc ggcggaatgc tcgatcacgg tttggaagtt attgcttttt
    10141 cagccaaagt ccgtcaaaac tatatattgc caccgaatgt cgcacctgaa gaacaatcta
    10201 agcaacgaga cgcttggact gc
//
LOCUS       DANLRK010000091        10220 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.091, whole genome shotgun sequence.
ACCESSION   DANLRK010000091 DANLRK010000000
VERSION     DANLRK010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 10220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10220
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.091"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            399..1751
                     /gene="lysC"
                     /locus_tag="PXA33_001636"
     CDS             399..1751
                     /gene="lysC"
                     /locus_tag="PXA33_001636"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HDL5120955.1"
                     /translation="MSHLSVAKFGGTSVANFDAMTSSANIVIADANTRVVVLSASAGV
                     TNYLVELANGCEKEHRDEVIAAVRQIQYNIIEKLQHQDAVKAEIDELLERIETLAESA
                     SLATSAALSDELISHGEMMSTKIFTQLLIERGHPAVWVDVRDVVATNSHYGKAAPNDE
                     KTQQQSDNVIKPLIASGKIVITQGFIGRDDEGKTTTLGRGGSDYSAALLAEVLNANDV
                     LIWTDVPGIYTTDPRVVPNAQRIDTMAFNEAAEMATFGAKVLHPATLLPAVRSNIPVY
                     VGSSKAPEQGGTWVTRDPQPRPTFRAIALRRNQILLTLSSLSMLHAQGFLANVFAILA
                     KHKISVDVITTSEVSVALTLDKTGSASSGADMLSAELLDELNAYCNVQVESDLALVAI
                     IGNNLHAQSGVAKKLFHTLENYNIRLISYGASSNNVCTLVKNAEADDVVRSLHKGLFE
                     "
     gene            complement(1822..4068)
                     /gene="parC"
                     /locus_tag="PXA33_001637"
     CDS             complement(1822..4068)
                     /gene="parC"
                     /locus_tag="PXA33_001637"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HDL5120956.1"
                     /translation="MTTEINYEGIEQMPIKRFTEDAYLNYSMYVIMDRALPFIGDGLK
                     PVQRRIIYAMSELGLNASAKYKKSARTVGDVLGKFHPHGDSACYEAMVLMAQPFSYRY
                     PLVDGQGNWGAPDDPKSFAAMRYTESKLSKIAEILLGELGQGTVDYQPNFDGSLEEPK
                     YLPARLPHILLNGTMGIAVGMATDIPPHNINELADASVMLLDNPKATLDDVLSVVQGP
                     DYPTEAEIITPKAEIARMYEQGRGSIKMRAVWKKEEGEIVISALPHQASPSKIIEQVA
                     TQMRNKKLPMVDDIRDESDHENPIRIVIVPRSNRIDFDALMDHLFATTDLEKSYRVNM
                     NMIGLDGKPAVKNLLTILNEWLSFRRTTVTRRLNYRLDKILNRLHILDGLMIAFLNID
                     EVIEIIRNEDEPKAELMARFNLTDVQAEAILNLRLRHLAKLEEHQLQAEKSELEKERD
                     ELQLILGSERRLNSLIKKEIQADAKAFASPRRSPLVERAESKAIAESDLTPTEDVTVI
                     LSEKGWVRCAKGHDIDVQALSHRAGDGYLAHARGRSNQPVVFLDSTGRAYALDPTSLP
                     SARSQGEPLTGKITLPEGAVVQQVLMASPETKVLMASDSGYGFICTFADLVSRNKAGK
                     AVISLTENAKVLPPQLLENDENLSLVAMSNVGRMLVFPVSELPQLSKGKGNKIINISA
                     AAAKSGDEYLARLLVIKPTNSLVFVSGKRKITLKPSDVDNYRGERARKGSQLVRGLST
                     NSTVEIVE"
     gene            4253..5290
                     /gene="pstS"
                     /locus_tag="PXA33_001638"
     CDS             4253..5290
                     /gene="pstS"
                     /locus_tag="PXA33_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005756281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HDL5120957.1"
                     /translation="MAKRFKLLTVLGISFSLSVAANAQTITGAGASFPYPIYAKWAGL
                     YEKETGNKINYQSIGSGGGQQQIIAKTIDFGASDDPMKGETLAEHKLLQFPAIIGGTV
                     PVVNIPELKSGELKLSGKVLADIFLGKITQWNDPAIVALNQDIALPAKTILVVRRSDG
                     SGTTFGWTNYLSKVSAEWKEKVGEGKSVKWPVGQGGKGNEGVAAYVKQLKYSIGYVEY
                     AYAKQNALAWVSMQNADGKFVQPSRETFAAAAANADWSQTAGMGVILTNEAGEKAWPI
                     TAASFILIHQHSDKPEQTKGVFKFFDWAFKHGKDAAAELDYVPLPDNVVEKIQTQWKT
                     EVKDGQGNSLY"
     gene            5371..6330
                     /gene="pstC"
                     /locus_tag="PXA33_001639"
     CDS             5371..6330
                     /gene="pstC"
                     /locus_tag="PXA33_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HDL5120958.1"
                     /translation="MHKHKIDNKCLNHPWVEGLFHHTTRFFALLVFALLVVVLVSLLL
                     GSWEALSRFGVSFLWENEWNPVNDRYGALVPIIGTLISAGIALVIAVPVSFGIATFLT
                     ELAPQWLKRPVGTAVEMLAAIPSIIYGMWGLFVFVPLFQEYVQPYLIDTFGQLPFIGM
                     LFSGAPFGIGLFSAGLVLAIMIIPFIASIMRDVFSVVPPLLKESAYGLGSTTWEVIWK
                     VVLPYTKTGVIGAIMLGLGRALGETMAVTFVIGNAFMLPDSVFSPSTSIASAIANEFN
                     EAGGLQKSALMSLGLILFLITSIVLAASRLLIARMGKQEGKRE"
     gene            6327..7175
                     /gene="pstA"
                     /locus_tag="PXA33_001640"
     CDS             6327..7175
                     /gene="pstA"
                     /locus_tag="PXA33_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005650601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HDL5120959.1"
                     /translation="MKNYQNRRFYCRKIKNVLMLSLAYSAVLFGLFWLVWIIVTLLQK
                     GIPALSWELFTRPTPAPNETGGLRNAIVGSILIVSTATLIATPIGILAGTYLSEYGRY
                     TKLAKFTRFLNDVLLSAPSIIIGLFIYAIYVAQVKHYSGWAGSLALAVLVIPIVVRTT
                     DSMLNLVPNNLREAAAALGCPRWRVITMISYRAARSGIITGVLLAVARISGETAPLLF
                     TALSNQFASLDMNAPMANLPVVIYQYAASPFADWNTLAWAGAMLITLFVLSLNIITRL
                     YFSKTK"
     gene            7185..7955
                     /gene="pstB"
                     /locus_tag="PXA33_001641"
     CDS             7185..7955
                     /gene="pstB"
                     /locus_tag="PXA33_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HDL5120960.1"
                     /translation="MLPTDLETKLHINNLNFYYGDFHALKNINMRIAKNKVTAFIGPS
                     GCGKSTLLRTLNRMFELYPNQRATGEINLDGENLLTSDTDISIIRAKVGMVFQKPTPF
                     PMSIYDNVAFGIRLFEKLPKSELNDRVEWALTKAALWNEVKHKLNQNGDSLSGGQQQR
                     LCIARGIAVKPEVLLLDEPCSALDPISTMKIEELINELKHDYTVAIVTHNMQQAARCS
                     DYTAYMYLGELIEFGDTKQIFDKPKFRQTEDYIKGKMG"
     gene            8039..8725
                     /gene="phoB"
                     /locus_tag="PXA33_001642"
     CDS             8039..8725
                     /gene="phoB"
                     /locus_tag="PXA33_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006996031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HDL5120961.1"
                     /translation="MNNKILIVEDERAIREMLSLYLSKQYQVIEAEDYQSAINKLGEK
                     PELILLDWMMPGRSGIHLIKYLKKQPETEKIPIIMLTARSSEEDCITGLNAGADDYIT
                     KPFSPQVLLARIEAVWRRSYDPPSNLINIDGLILDQQAQRVLVAEQEVKLSGTEYKLL
                     HFLMTHPEKVYRREQLLDFIWGNDIYVEDRTVDSYIRRLRKSLEPYGFERYIQTVRGS
                     GYRFSNHFQD"
     gene            8728..10008
                     /gene="phoR"
                     /locus_tag="PXA33_001643"
     CDS             8728..10008
                     /gene="phoR"
                     /locus_tag="PXA33_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HDL5120962.1"
                     /translation="MKVKLSLKHFLLELLLAAAVAGVFNLFFPAYFFWFSFCLLVLLI
                     WHHHTESSLLQLLRPNEQSGRSKVTTMEHLSQSAAFYHERLRQDKIKTLRLLSKLNKH
                     IQYLPDAIIICQENGEISWCNNVAQELFEFYWDKKSNKSIFNVIFYPEFKSYFRQQRH
                     HRPLVLLTHNKRYIELNLNRYDSNSRLIVARDVTSFVRLMHSRQTFLANMNHELRTPL
                     TILQGYLEMLENEPHNMALQQKALNAMKEQSQRMTNLLRQLSLLVKIEHSNDSEHNEV
                     QMSALISAIQKNTEILSQNQQILFDIEPNLTVWGDQEQLQSAVSNLIYNAIRHAGEGV
                     TIQICCQRDNQEPNTAIFSVQDDGIGIDAHHITHLTERFYRVDESRSNKTGGSGLGLA
                     IVKHALEQHHSTLEIESEAGKGSRFSFKLPLIKK"
BASE COUNT         2900 a         2249 c         2112 g         2959 t
ORIGIN      
        1 attacctaaa tagcattgga ttacctaaat attcattgac aaaacattaa taaaacggta
       61 tttctatgca cacttttcaa tactgttgaa aaagagtaat gggcttgcca ttacgggaga
      121 ggtgcaacac tcaggcaact tgattttagg caactagggc cgtttgagaa ttaagtgatg
      181 ggagtgattg ccgaggtgtg aattcactag actggaatta acatcggctg aataggttga
      241 atcctatcag ctgtcattta ataggttatt tcaccatatt aaatggagtg ctctgattaa
      301 ttggttcaac aataaaaccg tataatctac tcaaatttcc tttccgaata tcccctaaca
      361 caatatttat ctatttttgt tttaaggaaa tttttattat gtctcatctt tctgtggcta
      421 aatttggtgg tacaagtgtt gcaaattttg atgcaatgac ctcttctgcc aatattgtaa
      481 ttgctgatgc aaatactcgt gtggtggtac tttctgcctc tgctggcgta accaattact
      541 tagtggaact tgctaacggc tgtgaaaaag aacaccgtga tgaagtcatc gcagcagtcc
      601 gccaaattca atacaatatt attgaaaaat tacaacatca agatgccgtt aaagcggaaa
      661 tcgacgaatt attagaacgt attgaaacat tagcagaatc agccagcctt gctacttcag
      721 ctgccttaag tgatgagtta atcagccatg gggaaatgat gtccaccaaa atcttcacgc
      781 aattattaat tgaacgtgga cacccggcag tatgggtaga tgtgcgtgac gttgtggcaa
      841 caaacagcca ttatggaaaa gcagcaccaa atgacgagaa aacccagcaa caatcagaca
      901 atgtgattaa gccattaatc gcatcaggca aaattgtgat tacacagggc tttattggtc
      961 gtgatgatga aggcaaaacc actactttag gacgtggcgg ttcagactac tctgctgcat
     1021 tgttagcaga agtattaaat gctaacgatg tactcatttg gacagacgta ccggggattt
     1081 ataccaccga tcctcgtgtt gtaccgaacg ctcaacgcat tgacacaatg gcatttaacg
     1141 aagcggcaga aatggcaaca tttggagcga aagtattaca cccagcaacc ttattaccgg
     1201 cagtgcgtag caatatccct gtatatgttg gctcaagcaa agcaccggaa caaggcggca
     1261 cttgggtaac ccgtgatcca caaccacgcc cgactttccg tgcgattgca ttacgccgca
     1321 accaaatttt actcacgctt tccagccttt caatgctaca cgcacaaggt ttcttagcga
     1381 atgtatttgc gattttagcg aaacacaaaa tttcggttga tgtgattacc acttccgaag
     1441 tcagcgttgc attgacccta gataaaacgg gctcagcctc ttcaggtgca gatatgttat
     1501 cagcggaatt actcgatgag cttaacgcat attgcaatgt gcaagtagaa agcgacctcg
     1561 ctttagtcgc tattatcggc aacaacttac acgctcagtc cggtgtggcg aaaaaattat
     1621 tccacactct tgagaattac aacattcgcc taatcagcta tggagcaagc agcaataatg
     1681 tttgtacctt agtgaaaaat gcagaagcag acgatgtggt tcgttcgctt cacaaaggct
     1741 tatttgaata agcaataact ccattgcaag cggttatttg gggcaaaatt gttggattta
     1801 ccccctaacc gcttcttctc tttactcaac aatctctacc gttgaattgg tactaagccc
     1861 tctcactagc tgtgagcctt ttcttgctcg ttcgccacgg tagttatcaa catcgctcgg
     1921 ttttaatgtg attttgcgtt taccggacac aaagaccagc gagttggttg gtttgatgac
     1981 taataaacgg gctaaatatt catcaccgga ttttgccgct gcggcggaaa tattgatgat
     2041 tttattgcct ttacctttgg aaagttgagg taattcactc accgggaaaa ccaacattct
     2101 gccaacatta ctcatcgcta caagcgataa attttcatca ttttccaata attgcggcgg
     2161 cagtactttt gcattttcgg ttaaagaaat aaccgctttg ccggctttat tacgagaaac
     2221 taaatctgca aaggtgcaaa taaagccgta acctgaatcg gatgccatta gcactttagt
     2281 ttctggactt gccattagca cttgctgcac tactgcacct tccggtaggg tgattttgcc
     2341 tgtaagcggt tcgccttgcg agcgtgccga aggcagtgaa gttggatcga gagcataagc
     2401 tcgccctgta ctgtctaaaa ataccacagg ctgattactt ctaccccttg cgtgggcaag
     2461 ataaccatct ccggctcgat ggcttagggc ttgaacatca atatcgtgtc ctttcgcaca
     2521 acgtacccag cctttttcag acaaaatcac agtcacatct tcggttggag tcaagtcact
     2581 ttccgcaatc gctttagact ccgctcgttc aaccaatggt gagcggcgtg ggctggcaaa
     2641 tgctttggca tcggcttgaa tctctttctt gatcaggctg ttcaaacggc gttccgagcc
     2701 taaaatcagt tgcaattcat cacgttcttt ttccagttcg gatttttccg cctgtaattg
     2761 atgctcttcc aatttggcta agtggcgtaa acgtaagttc aggatcgcct ctgcttgcac
     2821 atcggtcaga ttaaaacgag ccattaactc ggctttcggc tcatcttcat tgcgaataat
     2881 ctcaatcact tcatcaatat tgagaaatgc aatcatcaaa ccgtctaaaa tatgcaagcg
     2941 gtttaaaatt ttatctaatc ggtaattcag gcggcgagtg acagtggtac gtctaaaact
     3001 cagccactca ttgagaatag tcagcaaatt cttcactgcc ggcttgccgt ctaagccaat
     3061 catattcata ttgactcggt agcttttctc caaatcggtc gtcgcaaaca ggtgatccat
     3121 taacgcatca aaatcaatgc gattagagcg tggcacaatc acaatgcgaa tcgggttctc
     3181 gtggtcagat tcatcacgaa tatcgtccac catcggtagt tttttattcc gcatttgggt
     3241 tgctacttgc tcaataattt ttgaaggaga ggcttggtgc ggcaaggctg aaatcacaat
     3301 ttcaccctct tcttttttcc acaccgcacg cattttaata gaaccacgcc cctgctcata
     3361 cattctggca atttccgctt ttggtgtgat gatttcagcc tctgtcgggt aatccggccc
     3421 ttgtaccacg cttagtacat catcaagcgt tgctttcggg ttatccagca acatcacact
     3481 ggcatcagcc aattcattaa tattgtgtgg tggaatatcg gttgccattc ccaccgcaat
     3541 ccccatcgtg ccattcaaca aaatatgcgg taaacgagcc ggcagatatt tcggttcttc
     3601 caacgagcca tcaaaatttg gctgataatc caccgttcct tgccctaact cgcccaataa
     3661 aatttctgca attttagaca gtttcgattc ggtataacgc attgcagcga aggatttcgg
     3721 atcgtccgga gccccccagt tgccttgtcc gtccactaac ggatagcggt aagagaacgg
     3781 ttgagccatt agcaccattg cttcatagca ggcactatca ccgtgcggat ggaatttacc
     3841 taatacatcc cccacggtac gggctgattt tttatacttt gccgaggcgt ttagcccaag
     3901 ttccgacatc gcataaataa tacggcgttg caccggtttc aagccatcgc caataaacgg
     3961 caatgcccgg tccataatta catacatcga ataattgagg taagcatctt cggtaaaacg
     4021 cttaatcggc atctgttcaa tgccttcata attgatttct gtggtcattt tttatcgata
     4081 tttatattaa aaaaacgagg gctattttct acgatttagc ctgtttagtc tagcctgaaa
     4141 cacaagcggt cgttttttct gattttttta ccattgtcat cattttgtca tattcttttt
     4201 ctacactaag cacgtgaaat taactcacgc taactttaag gagaacaata tgattgccaa
     4261 acgatttaaa ctacttaccg ttttaggcat ttctttttca ctttctgtcg cagcaaatgc
     4321 tcaaaccatc acaggagccg gtgcttcttt cccttatcct atctacgcca aatgggcagg
     4381 tttatatgaa aaagagaccg gtaataaaat caattaccaa tctatcggtt ccggtggcgg
     4441 tcagcaacaa attattgcca aaacaattga tttcggtgct tctgatgacc cgatgaaagg
     4501 cgaaacatta gcagaacaca aacttttgca atttcccgcc atcattgggg gaactgtacc
     4561 ggttgtcaat attcctgaac ttaaatcggg cgaactgaaa ctgtccggta aagttcttgc
     4621 cgatatattc ctaggcaaaa ttacacaatg gaacgatccg gcaattgtcg cacttaatca
     4681 agatatcgcc ctgccggcaa aaacaatttt agtcgtacgc cgctcagacg gttcaggcac
     4741 aactttcggt tggacaaact atctttctaa agtctctgcc gaatggaagg agaaagtcgg
     4801 cgaaggaaaa tcggtgaaat ggccggtcgg acaaggcgga aaaggaaatg aaggtgtcgc
     4861 agcttatgtg aaacagctca agtattctat tggttacgta gaatatgcct atgctaaaca
     4921 aaatgctctg gcttgggttt ctatgcaaaa tgcagatggt aaatttgttc agccttcccg
     4981 tgaaacgttt gctgctgccg ctgctaacgc cgattggagc caaacagccg gtatgggcgt
     5041 tattttaacc aacgaagcgg gagaaaaagc ctggccgatt accgccgcca gttttatctt
     5101 gattcatcaa catagcgaca aaccagagca aactaaaggg gtatttaaat tcttcgactg
     5161 ggcatttaag cacggtaaag atgcggctgc cgaactggat tatgtgcctc taccggataa
     5221 tgtagtagag aaaatccaaa ctcaatggaa aacggaagtt aaagatggac aaggaaattc
     5281 gctctactaa tgcaaaacga ccgcttgtat ggagcttaac taacaacaag cggtcgtttt
     5341 ttcacaacat tttgcaaaag agttgtcact atgcataaac ataaaattga taacaaatgc
     5401 ttaaaccatc cttgggtaga ggggttattt catcacacaa cccgtttctt tgctttgctg
     5461 gtttttgcct tgttggtggt ggtgctggtt tcattattgc ttggcagttg ggaagcatta
     5521 agccgattcg gggtatcatt cctatgggaa aacgagtgga accctgtgaa tgaccgatac
     5581 ggtgcattag tgccgattat cggcacattg atttctgccg gcattgcatt agtcattgcc
     5641 gttccggtct cgttcggcat tgcgactttc ttaaccgaac ttgcaccgca atggctaaaa
     5701 cgccctgtcg gcacagcggt agaaatgcta gcggctattc cctctattat ttacggtatg
     5761 tgggggctgt ttgtatttgt acccttattt caagaatatg tgcaaccgta tttgatagat
     5821 acatttggtc agcttccctt tatcggtatg ctttttagcg gagctccttt cgggatcggg
     5881 ctattcagtg ccggcttggt attggcgatt atgattattc cctttattgc ctcaattatg
     5941 cgtgatgttt tcagtgttgt accgccttta ctgaaagagt ctgcttacgg tttaggctca
     6001 accacttggg aagttatttg gaaagtcgtg ctaccttaca ccaaaaccgg agtaatcggt
     6061 gcaattatgc tcgggttagg tcgagcatta ggcgaaacaa tggcagttac ctttgtgatc
     6121 ggtaatgcct ttatgctacc tgattcggta ttttcccctt caacctccat cgcttctgct
     6181 atcgccaatg aatttaatga agccggcgga ttacaaaaat cagcgttgat gagcctaggg
     6241 ctaattctgt tccttattac ttcgattgtt ttagccgctt cacgtttatt aatcgcacga
     6301 atgggtaaac aggaaggaaa acgagaatga aaaattacca aaaccgacgc ttttattgcc
     6361 gaaaaataaa aaatgtcttg atgttaagtc ttgcctattc cgctgtatta tttggcttat
     6421 tttggctggt ctggattatt gttactttac tccaaaaagg tatccctgca ctgtcttggg
     6481 aactgtttac ccgcccgact cctgcaccaa atgaaacagg aggattacgt aatgcaattg
     6541 tcggctctat actgattgtc agtaccgcaa cactgattgc aacaccaatc ggtattttag
     6601 ccggcactta cttgagcgaa tacgggcgtt ataccaaatt ggcaaaattc acccgttttt
     6661 tgaatgatgt gttgctctct gctccttcta ttattatcgg cttatttatt tatgcgattt
     6721 atgtcgcaca ggttaaacac tattcaggct gggcaggctc attagcattg gcggtattag
     6781 tgattccgat tgttgttcga actaccgaca gtatgctcaa tcttgtgcct aataatctgc
     6841 gtgaagcagc tgcggctctc ggctgcccac gatggagggt aattacaatg atctcttatc
     6901 gtgccgcccg ctccggcatt attaccggcg tgttgctcgc tgttgcccgt atttccggag
     6961 aaaccgcacc gctattgttt accgcattat ccaaccagtt tgcctcttta gatatgaatg
     7021 caccaatggc aaacctccct gtggtgattt atcaatacgc agccagcccg ttcgctgact
     7081 ggaatacatt ggcttgggca ggtgcaatgc ttattactct gtttgtgctt tctcttaata
     7141 tcatcactcg tctgtatttc agcaaaacaa aataaggact ctatatgtta ccaacggatt
     7201 tagagacaaa attacatatt aacaacctca atttttatta tggcgatttc cacgctctga
     7261 aaaatatcaa tatgcgtatt gccaaaaata aagtgaccgc atttatcggt ccttccggtt
     7321 gtggaaaatc aacattactg cgaaccctaa accgaatgtt tgagctctac ccgaatcaac
     7381 gtgcaacggg ggaaatcaat cttgatggag agaatctact cacctccgat acggatattt
     7441 cgattattcg agccaaagtc ggtatggttt tccaaaaacc gaccccattc ccgatgtcca
     7501 tttacgataa tgttgctttt ggtattcgtc tgtttgaaaa attgccgaaa tccgagctaa
     7561 acgatcgtgt agagtgggca ttaaccaaag cggctttatg gaatgaggta aagcataaat
     7621 taaaccaaaa tggtgacagt ctctccggcg ggcaacagca acggctctgt attgcacgag
     7681 gtattgcagt gaaacccgag gtgttactgc tggatgagcc ctgctccgca cttgacccga
     7741 tttcgactat gaaaatcgaa gaacttatca acgaactcaa gcacgactac acggtagcga
     7801 tagttaccca caatatgcag caagcagccc gctgttctga ctataccgct tatatgtatc
     7861 taggcgagct gattgaattc ggtgatacca aacaaatttt cgacaaacca aaattccggc
     7921 agacggaaga ttacattaaa ggtaaaatgg ggtaatctgc aagcggtcgg ttttagtaaa
     7981 aaatttgcaa atttctgcga taaatcgacc gcttgtcaca ccgcatcgga gaggaagaat
     8041 gaataacaaa attctgatcg ttgaagacga acgtgctatt cgtgaaatgc taagtctcta
     8101 tctcagcaaa caatatcagg taattgaagc ggaagactac caaagtgcta tcaataaact
     8161 gggcgaaaaa cccgagctta ttttactgga ttggatgatg ccggggcgtt cgggtatcca
     8221 tttaattaaa tacctaaaaa aacagcctga aaccgaaaaa attccaatca ttatgctaac
     8281 agcccgcagt agcgaagaag actgtattac cggtctgaat gccggtgcag acgactacat
     8341 taccaaaccg ttttcaccac aggtgttgct tgcccggatt gaagcagtat ggcggcgaag
     8401 ctatgatccg ccaagcaatc tcatcaatat agacggattg attcttgatc aacaggcaca
     8461 acgggttttg gttgccgaac aggaagtgaa attaagcggc acggaatata aactgcttca
     8521 ctttttaatg actcaccctg aaaaagtgta tcgtcgtgaa cagttgcttg attttatttg
     8581 gggtaatgat atttatgttg aggatcgcac ggtagactct tatattcggc gattgcgtaa
     8641 gagtcttgaa ccctatggtt ttgagcgtta tattcaaacc gtacgtggct cgggataccg
     8701 tttttccaac cattttcagg actaataatg aaagttaagc tctcacttaa acacttttta
     8761 ttggaacttt tgctggcagc agccgttgcc ggcgtattca atctgttttt ccctgcttat
     8821 tttttctggt ttagtttttg tttactggtt ctacttattt ggcatcacca caccgaatct
     8881 tctctccttc agttactcag acctaatgag caaagcggca ggtcaaaagt aacgacaatg
     8941 gagcatttat ctcaaagtgc cgcattctat cacgaacgtt tacgccaaga taaaatcaaa
     9001 accttacgtc tgttatcaaa attaaataag catattcaat acttacccga tgcgatcatt
     9061 atttgccaag aaaacgggga gatttcttgg tgcaataatg ttgcacaaga gctatttgag
     9121 ttttattggg acaaaaaatc aaataaaagc atttttaatg tgatttttta tcctgaattt
     9181 aaaagctatt tccgccaaca acgacaccat cgcccattgg tactgctgac tcataataaa
     9241 cgctatattg aattgaatct caatcgatat gacagtaact ccagacttat tgtcgcacga
     9301 gatgtaacct cttttgtccg ccttatgcac tcacgacaaa cctttttggc aaatatgaac
     9361 cacgaactgc gaacacctct taccatatta caaggttatt tggaaatgct ggaaaacgag
     9421 ccgcataata tggcattaca gcaaaaagcg ctcaatgcga tgaaagagca aagtcaacgt
     9481 atgactaacc tgcttcgcca actctcactt ttggttaaaa tcgagcattc caatgattcc
     9541 gaacacaatg aggtccagat gtcggcgtta atctccgcca tacaaaaaaa taccgagatt
     9601 ttaagccaaa atcagcaaat actgtttgat attgagccta atttaacggt atggggagat
     9661 caagagcaac tccaaagcgc tgtatcaaat ttaatttaca atgcaatacg gcacgccggt
     9721 gaaggagtta ccattcaaat atgttgtcaa cgtgataatc aagagccgaa tactgcgatt
     9781 ttcagcgtgc aagatgacgg tattggtatc gatgcccatc atattaccca tctgacagaa
     9841 cgtttctatc gtgtggacga atctcgtagc aacaaaaccg gcggtagcgg tttaggtctg
     9901 gctattgtca aacacgctct tgaacagcac cattccacac tggaaattga aagtgaagcc
     9961 ggtaaaggaa gcagatttag ttttaaacta cctctaataa aaaaatagcg atgcaatcaa
    10021 actattttca caaggctttt cacaaactaa tccccatttt acactgaagt tttgaatttc
    10081 tttataactg aggtgcaaga tcaaaataat aggcgatttt ttataaaaat cacgctatac
    10141 tagacaagtt ttatttcttg ttattgagag gcggctatca aaaatgtttt acttaaaagc
    10201 ctttttgcta gtcgcaattt
//
LOCUS       DANLRK010000092        10101 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.092, whole genome shotgun sequence.
ACCESSION   DANLRK010000092 DANLRK010000000
VERSION     DANLRK010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 10101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10101
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.092"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            99..1040
                     /gene="argC"
                     /locus_tag="PXA33_001644"
     CDS             99..1040
                     /gene="argC"
                     /locus_tag="PXA33_001644"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HDL5120963.1"
                     /translation="MSKYKIFVDGAVGTTGLRIHERLSQADDIELLTLPEEQRKDLNA
                     RVAKVAEADLTFLCLPDEASKELVANAPENTRICDTSTAHRVNPNWVYGFPELSGQRE
                     KIQNAKRVAVPGCHATGYIALVRPLVEKGILPADYPLSCHSLTGYSGGGKAMIADYEN
                     ENSPNAFNAPRVYGLTLQHKHLPEMQAVTQMQNPPIFTPVVADYYSGMLVTVPLQIFA
                     LQAVKSGEEIANLLADYYQTSRLIHVHPFQSLPEDGMLAANAYAGKDSLEIFIYGNST
                     QLLLAARFDNLGKGASGAAVQCMNIMLGRDEVEGLEL"
     gene            1132..1986
                     /gene="argB"
                     /locus_tag="PXA33_001645"
     CDS             1132..1986
                     /gene="argB"
                     /locus_tag="PXA33_001645"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005611371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HDL5120964.1"
                     /translation="MQQQEINQFANLLEKSTACLVKWQGKIIVVKYGGNAMINEELKK
                     SVMADLLLLHRLGVKVVLVHGGGLEISQGVKLLGKEPQFINGLRVTDSDTINVVLQML
                     AGKVNKSLVALLKGKGIGLCGIDGAMLQCKKLEAEVDYGFVGDIVKVDPTVIEIALNA
                     GLIPVVSTVGVDENGQAYNINADTAASEIAIALQAEKLVSMTDIAGLLRDRFDESTLI
                     PEVEVSEVQSLIEQGIIAGGMIPKIACCTDFIRAGGSEANIIDGRIDHAILVEMFGGK
                     NGTRFYKK"
     gene            1999..3183
                     /locus_tag="PXA33_001646"
     CDS             1999..3183
                     /locus_tag="PXA33_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HDL5120965.1"
                     /translation="MTSSNTIKQLDKDYIAQTYGRFDLALSHGKGCEVWDFEGNKYLD
                     FTSGIGVNSLGWADEDWLQAVTTQAGKLAHTSNLFFTEPASQLAQKLVAASGLKRAFF
                     CNSGAEANEGAIKTARKYSFDKYGSGRSTVLTLVNSFHGRTISTLASTGQEVFHKHFF
                     PFTEGFEYLPVNGIEALKARLETENVCGILLEVVQGEGGVCPLEADYLHAVQSLCQEK
                     DILFMIDEVQTGIGRTGTLFAYQQFDLTPGLVTLAKGLGGGLPIGAFLLGEKVEMTLG
                     KSDHGSTFGGNPVSCAAANVVIDKINAEFLAQVQQKGQLLQQRLASLPKVKSVSGLGL
                     MLGVEFEEGILAADIVAKCINKGVLFLTAKSKLRLLPPLMISEQQIEQGIRVLEDVLK
                     DS"
     gene            3366..3797
                     /locus_tag="PXA33_001647"
     CDS             3366..3797
                     /locus_tag="PXA33_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL5120966.1"
                     /translation="MSNQIALLLMSHGSFARAALASAELIVGQQENVAAIGVVAVDNV
                     ADIKRQMEQTIAELNTDKGLIILTDIVGGTPMNLASSQLTHPNVFACSGLNLPLLLEV
                     LMSRDGNLQEIRENLPLAYQNGFSLRTQQDFVSQEDDDDLL"
     gene            3784..4251
                     /locus_tag="PXA33_001648"
     CDS             3784..4251
                     /locus_tag="PXA33_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL5120967.1"
                     /translation="MICFSRIDDRLIHGQVVTTWVNLHRIEQIIVLNDAVAADPTQKQ
                     ILTMAAPQGIKVVAFGIEKFGEVLQKTQITRRTMLLFTNSIDVLRTVELGVSIDKLNV
                     GGMRYQARREQLSKAVSVTPEERQAFKTLLEKGVEVTVQMVPNDEKLNLQEII"
     gene            4254..5021
                     /locus_tag="PXA33_001649"
     CDS             4254..5021
                     /locus_tag="PXA33_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL5120968.1"
                     /translation="MLTALLVACWAGICALDDIGTQMIRRPLLIAPVVGLIMGDLETA
                     LIIGATLEVMWMGIGNVGAYSAPDMISGTAIGTALGIAAGEASTAVALAVPTSLLAQQ
                     LLILYRSGVCALNPIAERWAASGDFDKIFRIHYIPMLIAFSIRAIPTFLAVYYGADII
                     AQAVSALPAKVITGLNVAGKIIPAIGIGLLMLMMIKKAELWTFLIAGFTLAVYLKLSV
                     LPITLIALPLALIYDLAVSKKAEAAPTKTADEEDYDL"
     gene            5018..5824
                     /locus_tag="PXA33_001650"
     CDS             5018..5824
                     /locus_tag="PXA33_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL5120969.1"
                     /translation="MSNKITKQDLNKVFWRMQVLNVTNNFQSMQAIGFLSSFIPVLKR
                     LYKDRPQEERAEAMKRHLKFFNSHVNSDALILGIAAAVEETTKEDEKETVTAIKTGLM
                     GPLAGLGDSLLKFTWLPICGSIGAALALSGNVLGPILMFLLYNSVNIATKYYGIHLGY
                     SKGVDLIHGAGNNILQRLGNMANVVGLMVIGALIASVVKVKVVSQIAAGDNVIKFQEM
                     LDKVMPGLLSLLLTVVIYWILKKTNGKYAATMILATMGICIGLVYAGILG"
     gene            5824..6861
                     /locus_tag="PXA33_001651"
     CDS             5824..6861
                     /locus_tag="PXA33_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HDL5120970.1"
                     /translation="MDSVYDIIRLEQGRYLFRQQPAQSVQIFFLDNPDSKDSKWLAET
                     DLAEFELKLSACEARPFFLIQSANGEQIVAERTLPVAGMNNFRDMGGYVAHQGKRVKW
                     GKLYRSDHLHNLRDEGVAYLDKLGIQTVIDYRSPNEVAKYPNPPINGREQTFRLDPNA
                     HTAELAAQFSADKHDEDRNLVNKIIAQKAEGNLINRYDIVMAQYRNFVEKAECQTAFA
                     EMLRLATDPENAPLVQHCRGGKDRTGFGAMLLLGILGVSKADIIADYMLTHYNRLARN
                     EEKMAIYRTFTQDQDVLDYLLSLIDTQPEFIEQSLNTIETQYGTIEQYAQRVLGITAK
                     EIEALRANYLA"
     gene            6951..8171
                     /gene="agp"
                     /locus_tag="PXA33_001652"
                     /pseudo
     CDS             6951..8171
                     /gene="agp"
                     /locus_tag="PXA33_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005628146.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
     gene            8479..>10101
                     /locus_tag="PXA33_001653"
     CDS             8479..>10101
                     /locus_tag="PXA33_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HDL5120971.1"
                     /translation="MSQDIQADLKKILALLSRNRRFQSLFLLLLRSEQFINSDALSTE
                     LQLSVRTVKKEIKLLKEKLEPYGIQIFSRPFYGYKLLIQFEELHTQLKQYFQISQPMT
                     VNNKFESRVNAILRRLLVSQRALKVEDFQSELYLSFTSALTKEFSQVKALLGKYGLIL
                     EAKPYHGMNIVGESYRKIMLTVRLYKYFTQLEHPFGIKEFEQCFACPMTEKIRERLAK
                     TIIHFNLVFSDIYAERFIIYLRYFYNQQPELLLPKLNFNYQQTNEYQFVIKLLEQLRE
                     QDSRFEFNPEVIRFLTYIAIASTDLYRFKDCNSDNYPLLLDKAWEIHRFIFTQFSAYL
                     NLSEIHDDTCRKDLLKMLIPIAMKMMLSISDDVDLGLFNQTELHRNPILAHTVEKLVD
                     DIEKNYGYSLSIREQYFIFDIFSGMVNRIELERKPLRLAIIALNGRLSTQQLKRNIKR
                     YFSDYILKIETKVLYELAIMEDKPYDYYLCNEYGKNMAIPYKPIYFADSSLNENEYAT
                     SLNEVFYHAYQYDEVLPSIKSIEIEAQYRLNEF"
BASE COUNT         3065 a         1883 c         2243 g         2910 t
ORIGIN      
        1 aaaatcaatt aatgtgaata attattcaaa aataaacttg agtttagcct taaatagcct
       61 tagactaccg agcagaaaaa gtcaaagaag gattttcaat gagcaaatac aagatttttg
      121 ttgatggggc agtaggaaca accgggctac gcattcacga acgtttaagc caagcggacg
      181 atattgaatt attaaccttg cccgaagagc agcggaaaga tttgaacgcc cgtgtggcaa
      241 aagtcgcaga ggcggattta actttcctct gtttgccgga tgaagcctca aaagaattag
      301 tagcaaatgc accggaaaat acccgaattt gcgatacttc aaccgctcat cgggtcaatc
      361 ctaattgggt ttatggtttt ccggaattga gcggacaacg tgaaaaaatc caaaatgcca
      421 agcgtgttgc ggtgccgggt tgccatgcca cgggctatat tgcgttggtt cgtccactgg
      481 tggaaaaagg gattctgccc gccgattacc ctttaagttg tcattcctta accggctatt
      541 caggcggtgg caaagcgatg attgctgact atgaaaatga aaacagccca aatgctttca
      601 atgcccctcg tgtttacggt ttaactctcc aacacaaaca tttgcctgaa atgcaggcag
      661 ttacccaaat gcaaaaccca ccgattttca cccctgtggt tgctgattat tacagcggaa
      721 tgttagtcac tgtgccattg cagatcttcg ccttacaagc ggtcaaatcc ggcgaagaaa
      781 ttgcaaattt gctcgccgat tactaccaaa ccagccgact aattcacgtt caccctttcc
      841 aatctctacc cgaagacgga atgcttgccg cgaatgctta tgcaggtaaa gacagcttag
      901 agatttttat ttacggcaac agcacccaac ttttacttgc tgcccgtttt gacaatttag
      961 gcaaaggggc atcaggtgcc gcagtgcagt gtatgaatat tatgttggga agagatgagg
     1021 ttgaggggtt agagctttaa atataaatgt agggacaggt cctgtgcctg tccgataatc
     1081 atcgcccata ttcggacggg cacaggaccc gtccctacaa gagaaagaaa tatgcaacaa
     1141 caagaaatca atcaatttgc aaatttatta gaaaaatcca ccgcttgttt ggtgaaatgg
     1201 cagggtaaaa tcattgtggt gaaatatggc ggtaacgcaa tgattaatga ggaactgaaa
     1261 aaatcagtga tggcagatct gctgttactt catcgtttag gcgtaaaagt ggtactggta
     1321 cacggtggcg ggctggagat ttcacaaggc gtgaaattat tgggtaaaga accgcaattt
     1381 atcaacgggt tgcgtgttac cgatagcgat acgattaacg tggttttgca aatgttggca
     1441 ggcaaagtta ataaaagcct tgtcgcctta ttaaaaggga aaggcattgg gctgtgtggt
     1501 attgatgggg cgatgttgca atgcaaaaaa ttagaggcag aggtggatta cggctttgtg
     1561 ggcgatattg tgaaagtcga tccaactgtg attgaaattg ctcttaatgc gggcttgatt
     1621 ccggttgttt ccacggtggg cgttgatgaa aacgggcagg cttataacat taatgcagac
     1681 accgctgcca gcgagattgc gattgcgtta caagcggaaa aattggtctc gatgaccgat
     1741 attgcaggtt tattgcgaga tcgctttgat gaaagcacct taattccgga agtggaagta
     1801 agcgaagtgc agtccctgat tgagcaaggc attattgccg gtgggatgat ccccaaaatt
     1861 gcgtgctgta ccgatttcat cagagctggt ggctctgagg caaatattat tgacgggcga
     1921 atcgaccacg ccattttggt agaaatgttc ggtggcaaaa acggcacacg gttttataaa
     1981 aaataacagg aaagtgaaat gacaagcagt aatacgatta agcaattaga taaggattat
     2041 atcgcccaaa cctatggcag atttgacctt gcactgtcgc acggaaaagg ctgtgaagtg
     2101 tgggattttg agggcaataa gtatcttgat tttaccagtg gcattggggt aaatagttta
     2161 ggctgggcgg atgaggattg gctacaagct gttaccacac aagccggcaa attagctcac
     2221 acctcaaacc tgttttttac cgaaccggca agccaattag cccaaaaatt agtggctgca
     2281 agcggtctaa aacgggcatt tttttgcaat tcaggggcag aggcaaatga aggagcaatt
     2341 aaaaccgccc gcaaatatag tttcgacaaa tacggttccg gtcgttcaac ggttttaacc
     2401 ctcgtgaact cttttcacgg acgaaccatt tcaaccctcg catccactgg gcaagaggta
     2461 ttccacaagc attttttccc ctttacagag ggctttgaat atttacctgt aaatggcatt
     2521 gaggcactga aagctcgctt ggaaactgag aacgtttgcg ggattttgct ggaggtggtg
     2581 cagggcgagg gcggtgtttg cccattagag gcggattatc tgcacgccgt gcaaagcctg
     2641 tgccaagaaa aagatatttt atttatgatt gatgaggtgc aaaccggcat tgggcgaacg
     2701 ggcacacttt ttgcttatca acaatttgat ttaactcctg gtttagtcac gctcgctaaa
     2761 ggcttgggcg gcggcttgcc gattggtgct ttcctgttag gcgaaaaagt ggaaatgaca
     2821 ttaggcaaaa gcgaccacgg ctcaaccttt ggtggcaatc ctgtttcctg tgctgctgca
     2881 aatgtagtaa tcgataaaat aaacgccgaa tttctcgccc aagtgcaaca aaaagggcag
     2941 ttgttacagc aacgcttagc gagcttacca aaagttaaat ccgtttcagg cttaggttta
     3001 atgctcggcg tagaatttga ggaaggcatt ttagccgctg atattgttgc aaaatgtatc
     3061 aacaaaggtg tgctgttttt aaccgctaaa agcaagttaa ggctactccc accgctgatg
     3121 atctctgaac aacagataga acaaggaatc agagtgttag aggatgtctt gaaagattct
     3181 tgagttatta taaacagtag gaatatcttc ttactgtttt ttattactat tatatagtag
     3241 cctactttaa tctgttgtca ctttatattt cttcttatgt gtacgatata tcgtgcaata
     3301 acttcccttg tttatctgta taacatttta tttaatagaa tgacttataa atcaaggagt
     3361 gacttatgtc taatcaaatt gcgttattgt tgatgagcca cggcagtttt gcgagggcgg
     3421 cgttggcgag tgccgagctg attgtcgggc agcaggaaaa tgtggcagct atcggcgtag
     3481 tagcagtgga taatgtagcg gacattaaac ggcagatgga gcaaaccatt gctgagttaa
     3541 acaccgacaa aggcttgatt attctcaccg atattgttgg tggtacgccg atgaatcttg
     3601 cctcttctca attaacccac cctaatgttt ttgcctgttc gggtttgaat ttacctttat
     3661 tgctagaagt gctgatgagc cgtgacggca atttgcaaga aattcgggaa aatctaccgc
     3721 ttgcatacca aaacggattt agcctacgca cacaacaaga ttttgtttca caggaggacg
     3781 atgatgatct gctttagccg tattgatgac cgtttaattc acggtcaagt agtcaccact
     3841 tgggttaatt tacaccgtat tgaacaaatt attgtgttaa atgatgcggt ggcagcggat
     3901 ccaacccaaa aacagatttt aacgatggca gcaccgcagg gaattaaagt ggtcgcgttt
     3961 ggcattgaaa aatttggtga agtgttgcag aagacacaaa ttacccgccg tactatgttg
     4021 ttgtttacca atagcattga tgtgttacgc actgtagaac taggtgtatc gattgacaaa
     4081 ttgaatgtcg gcggaatgcg ttatcaagct aggcgtgagc agttaagtaa agcggtttca
     4141 gttacgccag aagagcgtca agcatttaaa accttattgg aaaagggtgt tgaagtaacg
     4201 gtacagatgg taccgaatga tgaaaaactt aacttacagg agattatcta gctatgctga
     4261 ctgcactact tgttgcctgc tgggcaggta tttgtgcctt agatgatatt ggcacacaga
     4321 tgatccgccg tccgttgtta atcgcaccgg tggtcggatt gattatgggg gatttggaaa
     4381 cggcgttgat tattggtgct acgcttgaag tgatgtggat ggggatcggc aatgttggtg
     4441 catattctgc accggatatg atttccggaa ccgctattgg caccgcactt ggtattgctg
     4501 ctggtgaagc ttctactgcg gttgcactcg cagtaccgac ctcactcttg gcacagcagt
     4561 tactcattct ttaccgctcc ggcgtatgtg cattgaaccc aattgctgaa cgctgggcgg
     4621 caagcggtga ttttgacaag attttccgca ttcactatat cccaatgtta attgcctttt
     4681 ctattcgtgc tattccaacg ttcttagccg tctattacgg ggcggacatt attgcccaag
     4741 cggtctctgc tctgccggca aaagtgatta ccggattaaa tgttgcaggc aaaattattc
     4801 ctgccatcgg tatcggtttg ctgatgttaa tgatgattaa gaaagccgaa ctttggacgt
     4861 tcttaattgc tggctttacg ttggcggttt atctcaaatt atcagtattg ccgattacct
     4921 taattgcact accgcttgcg ttgatttacg acttagcagt aagcaaaaaa gctgaagctg
     4981 caccaaccaa aaccgcagat gaagaggatt atgacctatg agcaataaaa ttaccaaaca
     5041 agatttaaac aaagtgtttt ggcggatgca ggtgctgaac gtcacaaaca acttccaatc
     5101 gatgcaggcg attggatttt taagtagctt tattccggta ttaaaacgac tttataaaga
     5161 tcgtccacag gaagaacgag cggaagcaat gaaacgccac ttaaaatttt ttaacagcca
     5221 cgttaattct gatgcattga ttttaggaat tgccgctgcg gtagaagaga ctaccaaaga
     5281 ggatgaaaaa gagacagtaa ccgcgattaa aaccggttta atggggccgt tagcagggct
     5341 gggagacagc ttgttgaaat tcacctggtt accgatttgc ggtagtatcg gggcggcatt
     5401 ggcactaagt ggcaatgtgt taggcccgat tttaatgttc ttgttgtata acagtgtaaa
     5461 cattgcgacg aagtattacg gtatccatct aggttacagc aaaggggttg atttaattca
     5521 cggtgcgggt aataacattt tgcaacgact cggcaatatg gcaaatgtgg ttggtttaat
     5581 ggtgattggc gcattaattg cctccgttgt gaaagtcaaa gtagtttcac aaattgccgc
     5641 tggtgataat gtgattaaat tccaagagat gttagataaa gtgatgccgg gactactcag
     5701 cttactttta actgtcgtga tctactggat tttgaagaaa actaacggca aatatgctgc
     5761 aacgatgatc cttgctacga tggggatctg tatcggctta gtctatgcag gaatactcgg
     5821 ctaatggaca gtgtgtatga cattattcgg ttggaacagg gcagatacct gttccgccaa
     5881 cagccggctc aatcggtaca gattttcttt ctggacaacc ctgacagtaa ggatagcaaa
     5941 tggctggcgg agactgattt ggcagagttt gagctgaaat tgagcgcttg tgaagcccgt
     6001 ccgttcttcc tcattcaatc agcaaacgga gaacagattg ttgctgaacg caccttacct
     6061 gttgccggaa tgaataattt tcgggatatg gggggctatg tcgctcatca gggcaagcga
     6121 gtgaaatggg gcaagttata ccgctccgat catctgcata atttaagaga cgaaggcgtg
     6181 gcttatcttg ataaattggg aattcagact gttattgact atcgcagccc gaatgaagtc
     6241 gccaaatacc caaatccccc gattaatggc agagagcaaa cttttcggct tgatccgaat
     6301 gcacatactg ccgagcttgc tgcccaattt agtgcagaca agcacgatga agatcgtaat
     6361 ttggtcaata aaatcattgc tcaaaaagct gaaggtaatc ttatcaatcg ctacgacatt
     6421 gtaatggcac aatatcgtaa ttttgtcgaa aaagcagaat gccaaaccgc ctttgctgaa
     6481 atgttaaggt tggcgacaga ccctgaaaat gcaccattgg ttcaacactg ccgtggaggg
     6541 aaagatcgca ctggtttcgg tgcaatgtta ctgcttggta ttttaggcgt gagtaaagct
     6601 gatattattg ctgattatat gcttactcat tataaccgcc ttgctcgtaa cgaagagaaa
     6661 atggcgattt accgtacctt cactcaagat caagatgtgt tggactacct attgtcttta
     6721 atcgatactc aacccgaatt tatcgaacaa tcgcttaata ctattgaaac ccaatatggt
     6781 actattgagc aatatgccca aagggtatta ggtattactg cgaaggaaat tgaagcatta
     6841 cgggcaaatt atttggcgta gaacggtagg gtgtaatatt gaacacacta caaatggaga
     6901 aatttcatat gcgttatttt gcaaaacatt ggtataaaac taccgcttgt ctgacaacaa
     6961 ttttcacagc atttcctctt tttgctcaac caattgaaaa tataccaact tcccactacg
     7021 aattagaaaa aatcattata tttagccgac acgggttacg ttcccctctt acagaaaaag
     7081 gcagtgattt agaaaaatca accccttata aatgggcaaa ttggagtgta ccgaccggtc
     7141 atttaaccca taaagggacg ctgttggaaa cctattttgg gcagtatctt ggtcaatggc
     7201 tcacacataa tgggttattg gacaaggcaa gctgtgaaag tgggaagcag attgaggttt
     7261 atgctaatag cctgcaacgt acaattggca ctgcacaagc tttggttgcc ggtgggtttt
     7321 caggttgtaa tgtacaagtt aaacaccacc agcagattgg aaagatggat ccggtgttta
     7381 atccaattat tcgctccgat gaccagacat ttgtgcaaac agcattaagc aaaattgacc
     7441 tcaataaaac ccatcagtat ttagcgccat cctatcattt attaagcgaa ataatcgatt
     7501 atgctcattc agaaaaatgt ttgcaggaaa aacagtgtga attttttaat gatgttggta
     7561 aattaaggat taaaaaggat aaataaccag gtgtttccgg tacgatgcga ttaagtaaaa
     7621 atattgtcga tgccttattg ctgcaatatt atgaaggttt tcctttaaaa gagattgcaa
     7681 ataaccagct tgatagtgat cagaaatggc aaacactcaa taaaattaaa aacgattacc
     7741 aatttatgct acgaggagat ccatttattg ctaaacatat ttcattaccg ctattaaaat
     7801 atattcagca agatttaaat agtgaaaata aaataacact tttagtcgga cacgattcca
     7861 atattatagc cttactttct gcattaaatg ttaagcctta taaattagca catcaatatg
     7921 aacaaactcc aataggaggg aaagtcttta ttgaaatctg gaaagaaaaa agtagccaag
     7981 cacaaaaagt taaactggaa tatgtatatc aatctactga ccaaattcgt aaagcgattc
     8041 cactaagcct tgataaacca ccgcaacatc acattttgag tttggattat tgccaagctg
     8101 accaatatgg attatgcgat tatatggaat ttaaacagtt actggaaaaa gtcattgctg
     8161 atacattgtg agattttgac aaagtgagtg aaaaaggtag cataaaaaag tcgggacagt
     8221 tgccagctgc tccctgtaag atgttgttta cagcactagt aatgctacta tccccaaaaa
     8281 gctgctgaaa ggcagctttt ttgttgtata taaaagtaca cctttctacg cttcattctt
     8341 tttctccaat taaacctaat ttaggacttc tagtgataaa ttcggtatca tatcttaatt
     8401 cactacatat cgtgcaagcc ttaaaattga aagggctgac gaaatcctat ttaattctga
     8461 cacacataag ggaatattat gtcgcaagat attcaagccg atttaaaaaa gatattggca
     8521 ctattaagcc gtaaccgtcg atttcaatca ttatttttat tactgttaag aagtgaacaa
     8581 tttattaata gtgatgcact ttctacggag ttacagcttt cagtacgaac ggtaaaaaaa
     8641 gagattaagt tattaaaaga aaaacttgaa ccttacggca ttcagatttt ttctcgaccg
     8701 ttttatggct ataaattatt gattcagttt gaagaattac acacgcaact aaaacagtat
     8761 ttccaaatat cacagccgat gacggtaaat aataaatttg agtctcgggt aaatgcgatt
     8821 ttacgccgat tactggtgag ccagcgtgca ttgaaagttg aagattttca gagtgagctt
     8881 tatttaagtt ttaccagtgc cttaacaaaa gaatttagtc aagttaaagc cttgttagga
     8941 aaatatggat tgatactcga agcgaaaccc tatcacggaa tgaacattgt aggtgaaagt
     9001 tatcgtaaaa taatgctcac agtacgatta tataaatatt ttacccagct tgaacatcca
     9061 tttggaatta aagaatttga acagtgtttt gcttgtccta tgactgaaaa aatccgtgaa
     9121 aggttagcta agacaattat tcatttcaat ttagtatttt ctgatattta tgctgagcgg
     9181 tttattattt acttacgcta tttttataat caacagcctg agttattgtt accgaagtta
     9241 aattttaatt atcaacagac gaatgaatat cagtttgtaa ttaagttact ggaacagctt
     9301 cgtgaacaag acagtcgttt tgagtttaat ccggaagtta ttcgatttct gacctatatt
     9361 gcgatagcca gtacagatct ctaccgcttt aaagattgta attcggataa ttacccattg
     9421 ttgctagata aggcttggga aattcatcgt tttattttta ctcagttttc tgcatattta
     9481 aatttatcgg aaattcacga tgacacttgc cgtaaagatt tgcttaaaat gctgatccct
     9541 atcgcaatga aaatgatgct tagcatttca gatgatgtgg atttagggct atttaaccaa
     9601 acagaactac atagaaatcc aattttggct catacagtag aaaaattagt ggatgacatt
     9661 gaaaaaaact acgggtatag cctatctatt cgagaacagt attttatttt tgatattttt
     9721 tccggaatgg ttaatcgaat tgaattagaa cgtaaaccgc tccgactggc gattattgcc
     9781 cttaatggca gattaagcac ccaacagtta aaacggaata ttaagcggta cttttccgac
     9841 tatattttga aaattgaaac caaagtgcta tatgagcttg ccataatgga agataaacct
     9901 tatgactact atctctgtaa tgaatatgga aaaaatatgg ctattcccta taagcctatt
     9961 tattttgctg acagtagttt aaatgaaaat gaatatgcaa catcactcaa cgaagtattt
    10021 tatcacgctt atcaatatga tgaggtgttg cctagcatta aatcgattga aattgaagca
    10081 caatatcgcc taaatgagtt t
//
LOCUS       DANLRK010000093         9885 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.093, whole genome shotgun sequence.
ACCESSION   DANLRK010000093 DANLRK010000000
VERSION     DANLRK010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9885
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.093"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            9..3167
                     /locus_tag="PXA33_001654"
     CDS             9..3167
                     /locus_tag="PXA33_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HDL5120972.1"
                     /translation="MITINENTIEQSAIATLQSLGWDYTYGKKILAGLEHEWRERTAE
                     VILKPLLAQAIAKFNPNLPACEVENVVAQVCRADSGDLAERNRQAYDWLRNGVKITYQ
                     LYGEQVSDVVQLIDFQRPENNDFRIVNQLDISGKKGKRIPDLIGFVNGLPLVVFELKN
                     PLKENADIGKAFAQLQTYKDEISDLFVFNQALVISDGIVARIGSLTADFDRFTPWRVV
                     DEKNQSKRIVFEDELTALLQGVMTPKNLLDYVQNFVVFERDGKNRLIKKIGAYHQFYG
                     VNEAVDCTLLAATGNRKIGVFWHTQGSGKSLSMLFYAGKVLSQSSLKNPTLVLVTDRN
                     DLDGQLYATFCGGEALLKQTPIQADGRDELRSALASRSAGGVIFTTIQKFGLMEGELA
                     HPVLNERENIIVITDEAHRSQYGFSQKINHKGEYREGYAKHLRSALPNASFIGFTGTP
                     IELDDKDTQEVFGKYVSIYDFEDAVEDGATVPIIYEPRQISLGESGEFSKVMEEAQQL
                     IDDDENSYNFRLREKLHSVDSRLQKMAEDIIAHYDERTKQQDGKAMVVVMSRAICVKL
                     YEKITALRPEWHSNDVLQGSIKIVMTSNASDPAEWQKHNQDKKTLEKRFKDPDDPLNI
                     VIVRDMWLTGFDAPCCNTMYIDKPMSGHNLMQAIARVNRVFRNKSRENGGLIVDYVGL
                     TDELEKAMKQYTNAGGKEKPVRDISAVLEKMVEHITVIRGQFATPIDGQAVDIAKMLQ
                     ISEPPKLLNAILTSANHILALDRIQPPDNTAKDKTPRKNAFLQSVRLAKKGYALCGAL
                     KAVEPYKQELAFYDAVRATIIKNSTAPRNSSSENDRLLQLTALMNRAVQSDGVVDLFD
                     LLKKDRPNINLLSDEFLETVKNSPTKDLWLSAMERYLASQLREQSGANLATKKAFEQK
                     LKEAMNQYHNHNLSVLEILEELIALAKEFEARQKRGEALGLSPAEMAFYDALARNESA
                     VREMGDEVLMKLAKDITDKLRKSVTVDWQYKDSVRAKMRTLIRIALRTYKYPPDLQAE
                     AIEFVLQQAEEIAGEFAITETA"
     gene            complement(3201..6092)
                     /gene="glnE"
                     /locus_tag="PXA33_001655"
     CDS             complement(3201..6092)
                     /gene="glnE"
                     /locus_tag="PXA33_001655"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HDL5120973.1"
                     /translation="MLTTLIPLSQLNLPPNLQNSEQIQPLVTAISLSNFVHQTLQKQP
                     DLLAEWLAKFPTPDDCCNYTERLAKILARVENEEMLGRVLRQFRHRELARLSFIQSNK
                     LATVELVFQHLSDLAESLILAARDWLFQRCCAEYGTPKNTLGETQELLILGMGKLGGR
                     ELNFSSDIDLIFTYPDIGETEGGRKSIENSKFFTRMGQRLIKVLDEITADGFVYRTDM
                     RLRPFGDSGALVLSFTAMEDYYQEQGRDWERYAMIKAKILGEDSQNLNHRYLKQMLRP
                     FVYRRYLDFSAIQSLREMKEKISREVVRRNLTDNIKFGAGGIREVEFIAQTFQMIRGG
                     RDKILQERSLLKVLPRLAELNLLTQTQVETLHNAYIFYRQIENVLQAIDDKQTQTLPT
                     DEASQARLVFACQSYYQQDLYSEKTHWVEHSFNDWQQFMAVLSQYQQAVRQIFNEIIG
                     EEETQANCNPVNEKLAEWKDILHYNIRLEDLSAVLKGYPKVAENDYTEIFRHFSTTFQ
                     DWVKRPIGVRGREVLRNLMPRIADSIFKGEDHLLLLPRVLNIVDKITTRTTYLELMLE
                     KEQILPQLLALCSKSVMIAEQIARYPMLLDELMSHRGLTDVQAFEKYQSALQDYLIRI
                     PEEDEEALIDGLRQFKQTQILRIAAADILGVLLVMKISDHLTYLAEAIINAVVNMAWK
                     QVSQRFGVPEHLEADEKGFAVIGYGKLGGIELGYNSDLDLVFLHNAPEDSQTVGGKKE
                     ISSHQFYLKLAQKINSIFNLNTSAGVLYEVDMRLRPSGEAGLLVSTFNAYEHYQKNEA
                     WTWESQALVRTRCVFGAENLKQAFEKIRQSTLAQPRASGQLRQEICEMRQKMYQHLSS
                     HSAEQFHIKQDQGGITDIEFIAQYLVLAHSHQHPKMAVWSDNVRIFDSAVECGILSSE
                     QSEQLQHCYTALRNKIHHLNLLRKDSVVDASEFTTERAFVREMWQRLLA"
     gene            complement(6264..6545)
                     /locus_tag="PXA33_001656"
     CDS             complement(6264..6545)
                     /locus_tag="PXA33_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006717394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HDL5120974.1"
                     /translation="MYYVIFAQDNPNMLEKRLEVRPAHLARLEKLQAEGRLLIAGPNP
                     TEDGSSVTGSTVIAEFNSLEEAQQWASQDPYVEAGVYSDVIIKPFKRVF"
     gene            complement(6547..7011)
                     /gene="yciA"
                     /locus_tag="PXA33_001657"
     CDS             complement(6547..7011)
                     /gene="yciA"
                     /locus_tag="PXA33_001657"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HDL5120975.1"
                     /translation="MKDKPYGKAPEGKLILRTLAMPSDTNANGDIFGGWIMSQMDLGG
                     AILAKELAKGRVVTVTVDKMIFHRPISVGDVVCCYGKLMHIGRSSMQVKVEVFIKQVY
                     EGNRERYLVTEALFTYVAIDKEGKPRAVPRENNPELDEALSLIDLTPEKQGE"
     gene            complement(7001..7558)
                     /locus_tag="PXA33_001658"
     CDS             complement(7001..7558)
                     /locus_tag="PXA33_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HDL5120976.1"
                     /translation="MKQLLEFIPLILFFVVYKMSGIQMASIALVIATIVQMIALKMLY
                     GKIEKQQIIMGGSVVFFGLLSAYFNEVKYLQWKVTIVYALFALVLLVSQFVFKTPLIK
                     KLLGKEIQLPEAVWNKINFGWAVFFLICMLLNIYISQYLSEEIWVDFKSFGIIGLTLV
                     ATVITGFVLYPYLKDAEKQEQKNER"
     gene            complement(7567..8376)
                     /locus_tag="PXA33_001659"
     CDS             complement(7567..8376)
                     /locus_tag="PXA33_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL5120977.1"
                     /translation="MAQALLYFTTKYLQSIEGYFMSIKFVDLLQQSWNFMRNQQAFSL
                     FAIVTIVVVQLVFILVSPNSPELMAESQPQQLQIDASKMVSILLPTILLGVVNLLINV
                     LMIFNIQSINDGNYRQFFQNAGNALKHFLPVLLLQFVMVLPLSLGASFAMASPETVII
                     ALPVLVVGFYFFIKLSLVIYAYLLEKPQKSLSESIKFTLQLSRGKMLPLILFCVISYL
                     LPGMLSRLFTVFGNDFIGIFITIVLSAVINVFMAIFSFRFYQVYRQMPAVR"
     gene            8447..8884
                     /locus_tag="PXA33_001660"
     CDS             8447..8884
                     /locus_tag="PXA33_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HDL5120978.1"
                     /translation="MLSLFDAGQRYLNTLPNQKKLANFMPDYHIIRLVKFSSKVMPAF
                     ACFALVWQYCFTDPTQSILANSVLTAVFAISIPFQGLYWLGKRARSPLSLSLLEWYES
                     LRQKLISAQVKLEETQAVPSFQDFANLLKLAEETWGDEYFDEL"
     gene            complement(9169..9591)
                     /locus_tag="PXA33_001661"
                     /pseudo
     CDS             complement(9169..9591)
                     /locus_tag="PXA33_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005693492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(9721..9885)
                     /locus_tag="PXA33_001662"
                     /pseudo
     CDS             complement(9721..9885)
                     /locus_tag="PXA33_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019518307.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
BASE COUNT         2901 a         2119 c         2067 g         2798 t
ORIGIN      
        1 gagttgtgat gattaccatc aacgaaaaca ccattgaaca atccgccatt gcaactttgc
       61 aaagcttggg ctgggactac acctacggca aaaaaatttt ggcaggttta gaacacgaat
      121 ggcgggagcg aaccgctgag gtgattttaa agccgctttt ggcgcaagcg attgcaaaat
      181 tcaacccgaa tttgcccgct tgtgaggtgg aaaatgtggt ggcacaggtg tgccgtgccg
      241 acagtggcga tttggcggag cgtaatcgtc aggcttatga ttggctaaga aatggggtca
      301 aaatcactta tcagttgtac ggtgagcagg tgtctgatgt ggtgcagctg attgattttc
      361 agcgtccaga aaataacgat tttcgcattg tcaatcagct ggatattagc ggcaaaaaag
      421 gcaaacgcat tccggatttg attggctttg ttaatggctt gccgctggtg gtgtttgagc
      481 tgaaaaatcc gctcaaagaa aatgccgaca ttggcaaggc gtttgcccaa ctgcaaacct
      541 ataaagatga aatttctgat ttgtttgtgt ttaatcaggc actcgtgatt tcagacggca
      601 ttgtcgcccg catcggttcg ctgaccgctg atttcgaccg ttttacccct tggcgtgtgg
      661 ttgatgaaaa aaatcagagc aaacgcattg tgtttgaaga tgaactcacc gccctgctgc
      721 aaggcgtgat gacacccaaa aatctcttgg attatgtgca gaattttgtg gtgtttgaac
      781 gggacggcaa aaaccgctta attaagaaaa tcggagcata tcatcagttt tatggcgtga
      841 atgaagcggt ggattgcacc ttgcttgccg ccacaggcaa ccgcaaaatc ggcgtgtttt
      901 ggcatacgca aggttcgggc aaatcgcttt cgatgctgtt ttatgcaggt aaagtattaa
      961 gccaaagcag cctgaaaaat ccaactttgg tgttggtaac cgaccgcaac gatttggacg
     1021 gtcagcttta cgccactttt tgcggtggcg aggcactgct caaacaaacg ccaatccaag
     1081 ccgatgggcg agacgaactc cgctctgccc ttgccagtcg ttcggcaggc ggtgtgattt
     1141 ttaccaccat tcaaaaattc ggcttaatgg agggtgagct tgcccaccct gtattaaacg
     1201 agcgggaaaa catcattgtg attaccgatg aagcacaccg ctcacaatat ggttttagcc
     1261 aaaaaatcaa ccacaaaggc gagtatcgtg agggttatgc caagcattta cgcagtgcat
     1321 tgccgaatgc ctcttttatt ggctttactg gcacaccgat tgaacttgat gacaaagaca
     1381 cccaagaagt cttcggtaaa tatgtctcga tttatgattt tgaagatgcg gtggaagatg
     1441 gggcaaccgt gccgattatt tatgagccac gccaaatcag cttaggcgaa agtggcgagt
     1501 tttccaaagt gatggaagag gcacaacaac tgattgatga cgatgaaaac agctataact
     1561 tccgcctgcg tgaaaaactg cacagcgtgg atagccgttt gcaaaaaatg gcggaagata
     1621 ttattgccca ttacgatgag cgtaccaaac agcaagacgg caaggcgatg gttgtggtga
     1681 tgagccgtgc catttgcgtg aaattgtacg aaaaaatcac cgcacttcgt ccagaatggc
     1741 actcaaacga tgtgcttcaa ggcagcatta aaattgtgat gacaagtaat gccagcgacc
     1801 ctgccgagtg gcaaaaacac aatcaggata agaaaacctt agaaaaacgc tttaaagacc
     1861 cagacgatcc gctcaacatc gtgattgtgc gggatatgtg gctgacaggc tttgatgcgc
     1921 cctgctgtaa cacaatgtac attgataaac caatgagcgg acacaacctg atgcaggcaa
     1981 tcgctcgggt aaatcgggta tttcgcaaca aaagccgtga aaatggcggc ttgattgtgg
     2041 attatgtggg cttgaccgat gagctggaaa aagcgatgaa gcagtacacc aacgcaggcg
     2101 gtaaagaaaa gccggtgcgg gacatttcag cggtgctgga aaaaatggtg gaacatatca
     2161 cggtcattcg tgggcaattt gccacaccga ttgacggaca agcggttgat attgccaaaa
     2221 tgttgcaaat cagcgaaccg cctaaactgc tcaatgcgat tttgacatca gccaaccata
     2281 ttcttgccct agaccgcatt cagccacctg ataacactgc caaagacaaa accccacgca
     2341 aaaacgcctt tttgcaatcg gtacgcttgg cgaaaaaagg ttatgctttg tgtggggcat
     2401 taaaagcagt tgaaccctat aaacaggaac tggcgtttta tgatgcagtg cgtgccacca
     2461 ttatcaaaaa cagcaccgct cctcgcaatt cttcgagcga aaatgaccgc ttgttgcagc
     2521 ttaccgcctt gatgaatcgt gcggtacagt cggatggtgt ggtggattta tttgatttgc
     2581 tgaaaaaaga ccgcccaaac atcaacctgc tttctgatga gtttttggaa acagtaaaaa
     2641 acagcccaac caaagatttg tggctatcag caatggagcg ttatcttgcc tcacaactgc
     2701 gagagcaaag cggtgccaat cttgccacaa aaaaagcatt tgaacagaaa ctcaaagagg
     2761 cgatgaacca ataccacaac cacaatttaa gcgtattgga aattttagaa gagctgatcg
     2821 ccctcgccaa agagtttgaa gcccgccaaa aacgagggga agcgttggga ctaagccctg
     2881 cggaaatggc gttttatgat gctttggcac gtaatgaaag tgcggtgcgg gaaatgggcg
     2941 atgaggtatt gatgaaactt gccaaagaca ttaccgataa attacgcaaa tcggtcaccg
     3001 tagattggca atataaagac tcggttcgtg ccaaaatgcg aaccttaatc cgcattgctc
     3061 tgcgtaccta taaatacccg cccgatttac aggcggaagc gattgagttt gtgttgcaac
     3121 aagcggaaga gatcgccgga gagttcgcta tcaccgaaac ggcgtaatct atattagggc
     3181 gtgcaatgta cgccctaaat ttatgctaat aacctctgcc acatttcccg cacaaatgct
     3241 cgctcggtgg taaattcact tgcgtccacc acgctgtctt tgcgtaataa gtttaggtgg
     3301 tggattttgt tacgcagtgc ggtgtagcaa tgttgtagct gttcgctttg ttcggaactt
     3361 aaaataccgc attctactgc gctgtcgaaa attcggacat tatccgacca tactgccatt
     3421 ttcgggtgtt ggtggctgtg ggctaatacc aaatattggg cgataaattc aatatcggta
     3481 atacctcctt gatcttgttt aatatgaaat tgctcggctg aatgcgagct taaatgttgg
     3541 tacattttct gtcgcatttc gcaaatttct tggcgtaatt gaccgcttgc acgaggctga
     3601 gcaagcgtgc tttggcggat tttttcaaag gcttgtttga ggttttccgc cccaaataca
     3661 catcttgtcc gcacgagggc ttggctttcc cacgtccacg cttcattttt ttgataatgc
     3721 tcgtaggcat tgaaagtgct gactaaaagc ccagcctcgc ctgatgggcg aagtcgcata
     3781 tccacttcat atagtacgcc ggcactggta ttaaggttaa aaatcgagtt aattttttga
     3841 gccaatttta agtaaaattg atggcttgaa atctcttttt taccgccaac agtttggcta
     3901 tcctctgggg cattatgcaa gaaaaccaag tcgagatctg aattatagcc cagttcaatc
     3961 ccgcctagct tgccgtagcc aataactgca aacccttttt catcagcctc taaatgttcc
     4021 ggcacaccaa agcgttggct gacttgcttc cacgccatat ttaccaccgc attaataatc
     4081 gcttctgcta agtaagtaag atgatcgctg atcttcatta ccagcagcac gcctaaaata
     4141 tcagcagcgg cgatgcgtaa aatttgggtt tgtttgaact gacgtaagcc atcaatcagt
     4201 gcttcttcat cttcttcagg aatgcggatt aaataatcct gcaaggcaga ttgatatttt
     4261 tcaaaagcct gcacgtcggt taagcctcta tggctcatca gctcatcaag tagcattgga
     4321 tatcgagcta tctgttccgc aatcataacc gacttactac acaatgctaa aagttgaggc
     4381 agaatctgct ctttttcgag cattaattcc aaataggtag ttcgggtggt gattttatcg
     4441 acaatattca acacgcgagg caataacaat aaatgatctt cacctttaaa aatgctgtcg
     4501 gcaattctag gcattaaatt gcgtagcacc tctcgccctc ttaccccaat cgggcgtttt
     4561 acccaatctt gaaaggtagt agaaaagtga cggaaaatct ccgtataatc attttctgca
     4621 acctttggat aaccttttag cactgcggat aaatcttcaa ggcgaatgtt atagtgcaaa
     4681 atgtctttcc attcggctaa tttttcatta accggattgc agttggcttg tgtttcctct
     4741 tcaccgataa tttcattaaa aatctggcgt actgcttgtt gatattggct caatactgcc
     4801 ataaattgtt gccaatcgtt aaaggaatgc tccacccaat gcgttttttc gctatataga
     4861 tcttgctgat aatagctttg gcaagcaaat acaaggcggg cttgcgaggc ttcatcagtg
     4921 ggtagggttt gagtttgttt atcgtcaatc gcttgtaata cattttcaat ttgacggtaa
     4981 aaaatatagg cattgtggag cgtttccacc tgcgtttggg ttaataaatt taattctgcc
     5041 aagcgtggta gcacttttaa caggctacgt tcttgtaaaa ttttatctcg cccgccacgg
     5101 atcatttgga aagtttgggc gataaactcc acttcccgaa tcccaccggc accaaattta
     5161 atattatcgg ttaaattacg gcgaaccact tcacggctga ttttttcttt catttcacgc
     5221 aacgattgga ttgcactgaa atccagataa cggcggtaaa caaaagggcg gagcatttgc
     5281 tttaaatagc gatgatttaa attttggcta tcctcgccta aaatttttgc tttaatcatc
     5341 gcataacgtt cccaatcacg cccttgctct tggtaataat cttccattgc agtaaaactc
     5401 agcaccaagg ctccgctatc accaaagggg cgaagtcgca tatcggtacg atacacgaaa
     5461 ccatcagcgg tgatctcatc taatacttta attaaccgct gccccattcg ggtaaaaaat
     5521 ttgctgtttt caatcgattt gcgtccacct tcggtttcac caatatcggg gtaggtaaaa
     5581 atcaggtcaa tatcggaaga aaaattaagc tctcgtccgc ccaatttgcc catacctaaa
     5641 atcagcagtt cttgtgtttc gccaagagtg ttctttggcg tgccatattc ggcacagcaa
     5701 cgctgaaaca accaatctcg agcggctaga atcaggctct cggctaaatc cgacaaatgc
     5761 tgaaatacca attctaccgt cgctaattta tttgactgaa taaagctcag acgagccaat
     5821 tcacggtggc gaaattgccg caaaaccctc cccaacattt cttcattttc cactcttgct
     5881 aatatttttg ctaaacgctc agtataattg cagcaatcat caggcgtagg aaacttggca
     5941 agccattctg ccagtaaatc cggctgtttt tgcaaggttt gatgcacgaa attggaaagc
     6001 gaaatcgccg tcacaagcgg ttgaatttgc tcagaatttt gcaaattggg cggtaaattg
     6061 agttgggata agggaattag ggtggtaagc ataaatttag gctcaaaatt gaaaatagat
     6121 agaattttac gctcgatcaa ttcccctctt tagcaaagag ggcttagggg agatttggca
     6181 gaggtaattt tactctcata cgaaacatta atatcgtaat caaatctccc ccagcccctc
     6241 ttttctaaag aggggagaaa aaattaaaac actcttttaa acggtttaat aatcacatcg
     6301 ctatacacac cggcttccac ataaggatct tggcttgccc attgttgagc ttcttcaagt
     6361 gagttgaact ctgcaattac agttgaaccg gttacgcttg agccgtcttc agtcgggtta
     6421 ggccctgcga ttaataatcg accttctgcc tgtaattttt ccaaacgagc caaatgagcc
     6481 gggcgaactt ctaaacgttt ttccaacata ttgggattat cttgggcaaa gataacataa
     6541 tacatactat tctccttgtt tttcaggggt taaatcaatt agcgataatg cttcgtctaa
     6601 ttcggggtta ttttctctcg gcacagcacg cggtttgcct tctttatcaa tcgctacata
     6661 ggtaaataag gcttcggtta cgagataacg ttcgcgattg ccctcataaa cttgtttgat
     6721 aaagacctcg actttcactt gcatcgaaga acgccctatg tgcattaact tgccgtagca
     6781 gcagaccaca tcaccgactg aaatcgggcg gtggaaaatc attttatcta ccgttaccgt
     6841 aacgactcta ccttttgcca actctttggc taaaattgca ccgcctaaat ccatctgcga
     6901 cataatccaa ccgccgaaaa tatcgccatt ggcgttagta tctgacggca ttgccaaagt
     6961 tcttaaaatc agtttccctt ccggggcttt tccatagggt ttatctttca tttttttgtt
     7021 cctgcttttc agcatctttt aagtaagggt aaaggacgaa accggtaata accgttgcga
     7081 ctaaggttaa gccaataatg ccaaaggatt tgaaatcaac ccaaatctct tccgataaat
     7141 attgactgat ataaatattc agcagcatac aaatcagaaa gaatactgcc caaccgaagt
     7201 ttattttatt ccacactgct tcaggcaact gaatttcttt gcctaataat tttttgatta
     7261 atggtgtttt gaacacaaat tggctcacta atagcaccaa tgcaaacaga gcgtaaacaa
     7321 tggttacttt ccattgtaaa tatttaactt cattaaaata ggcactcagt aagccgaaaa
     7381 agaccaccga gccacccata ataatttgct gtttttcaat tttgccgtag agcattttta
     7441 aggcgatcat ttgcacgatt gtagcgataa ccaatgcgat agacgccatc tgaataccgc
     7501 tcattttata cacgacaaaa aacagaatca gcggaataaa ttcaagtaac tgtttcattg
     7561 atactcctaa cgcactgccg gcatttggcg gtaaacttgg taaaagcgga agctgaaaat
     7621 cgccataaag acgttaatta ccgcacttaa aacaatggta atgaatatgc caataaagtc
     7681 attaccaaac actgtgaaca aacgagataa catccccggt aataaataag aaatcacaca
     7741 aaataaaatc agcggtagca tttttccacg tgataattga agcgtaaatt taatactttc
     7801 cgatagactt ttctgcggtt tctccaataa ataagcataa atcactaacg ataatttgat
     7861 aaaaaaataa aaaccgacta ccaacaccgg caatgcaata atcactgttt caggcgatgc
     7921 cattgcaaaa gatgcaccga gagaaagcgg taacaccatc acaaattgta acaacagcac
     7981 aggtaggaag tgctttaatg cgtttcctgc attttggaaa aattgacgat aattgccatc
     8041 gttaatcgat tggatattga aaatcattaa gacattgatc aaaagattga caacacctaa
     8101 taaaatagtt ggcaatagta tagacaccat tttagaggca tcaatttgaa gttgttgcgg
     8161 ttgcgactcc gccattagct caggcgaatt tggcgacact aaaataaaaa ccaactgcac
     8221 aaccacaatg gtgacaatgg caaacagtga aaatgcttgt tgattacgca taaagttcca
     8281 actttgctgc aataaatcga caaatttaat tgacataaaa tacccttcaa tagattgtaa
     8341 atattttgtt gtgaagtata gcaaggcttg agccaaactg cattaaccta ttgtagattt
     8401 taatcagtta gctaaaataa aagaaaaatt tgaaagagat aatcctatgt taagcttatt
     8461 tgatgcagga cagcgttacc tcaacactct gccgaatcag aaaaaattag ccaattttat
     8521 gccagattac cacattattc gcttggtaaa attttcctct aaagtaatgc ctgcttttgc
     8581 ttgctttgca ttagtttggc aatattgctt taccgatccg acccaatcta ttttagccaa
     8641 ttcggtactt actgctgttt tcgccatttc tattccgttt caagggttat attggcttgg
     8701 aaaacgggca agatcaccgt tatctttatc attattggaa tggtatgagt cgcttagaca
     8761 aaaattaatt tcggcacaag tcaaattaga agaaacacaa gcggtacctt ctttccaaga
     8821 ttttgcaaat ttactcaagc tagcagaaga gacttgggga gatgaatatt ttgatgagtt
     8881 ataaaataaa aaaagcccca cttaaaataa gtggggtaaa acttttgttc taattctgga
     8941 ggtttgaatt actgttttat tgtggggtac tgatcttttt tgcatagaga gaaagaaaaa
     9001 aatttgggcg gaaaaataac ggcgagcccc gtgaatacaa gggttcgccg ttattgtttg
     9061 gttggagcta tgcaaaaaac aaaatgagca ggattagaaa aaaacgagca gtttttgcgt
     9121 tattttttgc ataggtttga tcggttttaa aactatttta aatctgtttt aaactaaaga
     9181 atgcccgatg tgtaccactt gcccgatgat ttgtaggttt tgggcttggt ctgctgtaat
     9241 ctttattaac tcgtactctt ctcggttgtc tgaaataagc tctacaccgt cccatagcag
     9301 ttttacacgc ttaacccaaa cactgtcgcc gttttgtaca atgtaaatat tattgtcgcc
     9361 tcgtatctca ttacgactta aatcaactag tagctggtcg ccattatcaa tagtcggata
     9421 catcgagtct ccacttgccc aaaaaacagc acacttcttg gcattaacac caagcgtatg
     9481 caacaagcta tcagcataag gctcttgtcc atcaggtttt gttacacctt cattaaagct
     9541 cccaaagcct gcagatactt taatagatgc aaaactatca atcattgaga ttatgttatt
     9601 caaaacggtg cctgataatt cagatccttt gttttgctct tcaggtgtaa tctctcttac
     9661 tatgctatcc ttgattgcat ttattccact tttaaatccg agtgcttctt gggctttacg
     9721 aggcaatgaa gttatgtgat attcattccc cccacctttt actccaactg catcacgttt
     9781 tatccagagt tcttttaatg cctttcttgt aatgtttgtt gcttgctttg gaaggtcagg
     9841 caaatccaat tcttctagtt ctttggctga aaaccaaact ttatc
//
LOCUS       DANLRK010000094         9817 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.094, whole genome shotgun sequence.
ACCESSION   DANLRK010000094 DANLRK010000000
VERSION     DANLRK010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9817
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.094"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            123..1829
                     /locus_tag="PXA33_001663"
     CDS             123..1829
                     /locus_tag="PXA33_001663"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HDL5120979.1"
                     /translation="MNTSSFNRTWAKVILSALMRYGVKHFCIAPGSRSTPLTLAAVQL
                     QQTQYAECHTHFDERGLGFYALGIAKATDSPVAIIVTSGTAVANLYPAVIEASLSHQK
                     LIILSADRPTELIGCGVNQAIHQQGIFANYPVASVNLPKANEAYHASWLVSTIEQACT
                     TQAQQGGVLHINVPFEEPLYEADINEILNTPWLRPIQGWLNNAQTKWIDQQAIQSEVS
                     MHENWDYWRTKRGVIVVGKLPPEQGTGLKTWAETMGWCLLSDVQSCIEASLPYADIWL
                     SNETVKQRLLQADIVIQFGTQIVSKRVNEFLKAFQGEYWVVEQSHDYHNPNAHHQTRF
                     IAKAHHFLRVHPPLRQKPWLLEPLALSQFCSTFIEQQVGGSLSEASLAHNIQDVLAIN
                     GNLFIGNSLFVRLVDALCKLPEGYPVYTNRGASGIDGLIATMAGIAKGSDRPTVGVVG
                     DISALHDLNSIALLTKINQPTILFVVNNSGGALFDMLPVDSKAKDQYYRLAHNFEFAQ
                     VATMFGLEYIRPFTWADLKTKLKHAYARKGVTIVEIKVNEHDGSNMYKSLLKQISQAE
                     IA"
     gene            1878..2717
                     /gene="menH"
                     /locus_tag="PXA33_001664"
     CDS             1878..2717
                     /gene="menH"
                     /locus_tag="PXA33_001664"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HDL5120980.1"
                     /translation="MLSYQWHAQQGTPVVFLHGLLGSQQDWQAVLQHLQNFPQIRPLT
                     IDLPFHGNSKHITCADFANLRQQLHATLESLIGSRPFYLVGYSLGGRAALDYVLNMEN
                     PNLQGVVLEGANIGLSTENDRQIRRQNDRHWANRFRQEPIEQVLEDWYKQPVFADLSD
                     TERECYIDSRKHNQGEQIAQMLEATSLAKQPDYTEKLKQTDKNIVFFIGEKDQKFRKM
                     ASSHQLPTQLIANAGHNAHRANAKNFVEKLGLLILHSDSDLRSQSVKHNGAALLHKSP
                     SGA"
     gene            complement(2766..3443)
                     /locus_tag="PXA33_001665"
                     /pseudo
     CDS             complement(2766..3443)
                     /locus_tag="PXA33_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251475.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            3529..4191
                     /locus_tag="PXA33_001666"
     CDS             3529..4191
                     /locus_tag="PXA33_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012478341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heteromeric transposase endonuclease subunit
                     TnsA"
                     /protein_id="HDL5120981.1"
                     /translation="MRKTHSFQQVRKIKPTWMSVSGHIPFRNGVSIPYESTLERDFLM
                     YFTYLPSVDEVVSQPTTLPFVKNGITYTYTPDFFLSFTDGRKPMLIEVKPKAKWQKHW
                     KEWKEKWKAAIRFCQENGYVFHVYDEDRIRHLALFNLNYVQRYKRIQHEQEDIDAILA
                     QVKLMGNTTIDYLLSRFFAGSLYRMKGLQIIYHLLATKQLHCNWFLPLNEFTEVWGNN
                     DE"
     gene            4181..6109
                     /locus_tag="PXA33_001667"
     CDS             4181..6109
                     /locus_tag="PXA33_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015586984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HDL5120982.1"
                     /translation="MMSNSGFTEKRYHHRLDRGRIILQKGNIYLNREDGEQYELVDYM
                     DEPSQLLVRNLNTRTTKVVSIHQLENFKMNERTDLSVDVTAISNEYWEKAQQKYEAIK
                     PLLGMDQHRPYAVKARAEDVGVNPRTLYRWLQAYNSIGSIAGLVDQKRGWQQGNSRLT
                     PEQDKLIVQVINEFYLHKQRPTTEQTIREIRRRCKIEKVESPSKETIRMRILHISEEE
                     RLRKRGQREKARNKFKPKPNSFPDADYPLSVVQIDHTPVDLIIVDSKYRKPIGRPFLT
                     VAIDIYSRMIVGYYLSLDAPSVTSVAMCIARGILPKERLLLDLGLQGSEWNAFGYPVK
                     VHVDNGPDFQALDLSKSCSAHGIHLEFRPMGRPEYGGHIERVIGTFMKEVHSLAGTTF
                     SNIKERDSYDSEKEAIMTLDEFEKWLVHYIVNVYHKRVHSALGISPEQKWKIGIFGDE
                     NEVGCGYPQLPVDEQTLLLDFLPSITRTIQHNGVTIDGLRYYDVALNMYISDSDESGK
                     SKEFLFRRDPRNISKIWFYDPKLKRYFPIPFANQAMPEMSIWEYREVRSRIANKGDKY
                     INEQQVLDGLTEMREMVAESAQRTKKARRQAERQKMHKASKPIIETKVETKAVVPVVV
                     TSNLLALDDESLSFGEVD"
     gene            6109..6996
                     /locus_tag="PXA33_001668"
     CDS             6109..6996
                     /locus_tag="PXA33_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012478339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TniB family NTP-binding protein"
                     /protein_id="HDL5120983.1"
                     /translation="MQYEHVHEKFRHLVTADNQERIAFLDEPRWLGYGVAKDIMDNLV
                     SLMNKPKRPRMLNLLIVGDSNNGKTTLIRRFFDLYGQAYIDSDSNAIYPILLAEAPPS
                     ANEKELYISLLERFYVPYKPTDTIAKLRYQTIHLFREFRVKMLVIDEFHSLLVGTPRL
                     QRQVMNAIKMLCNELQIPIVGVGTRDAIRVLHTDRQHASRFDVAELPIWKLDKEFQKL
                     LFQFQGILPLRESSNLQSPELATKIHTISGGNLGNVHRLLTACAVQAITSGTEKITLD
                     IIEKNSWVQPTNGYRKIIG"
     gene            6954..8060
                     /locus_tag="PXA33_001669"
     CDS             6954..8060
                     /locus_tag="PXA33_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TniQ family protein"
                     /protein_id="HDL5120984.1"
                     /translation="MGTTNKRLSQNNRIKKGVIHTPIYSNESISSWLIRASFDCGTDP
                     LTFTGFYWEKHRLWTLDLDRGLENIEPTIYDDVQALSGKIQLSEYSLFRRLYPINADE
                     TLAKGQVRWVIPRSARNRTHHIGQQYCPLCLDESPHLRNDWRLAWNFGCLKHNMMLAN
                     RCFCCGTLYQPHLLSAEKRHLNHCHQCGGKLEQGTMEMSLAEKLTLQGINQVLEKDSG
                     ICFGYKVSGKVWFDMLRYCINLLRRASDRPNHALAKFVVNLQIDVENICSPKTGLSFE
                     QLPLGERTNLLVNAYHILGMEADSIRSAIYLCGGTQKTFMFEKYPDFWQEFMALAPAG
                     PNRTRKRTKPQNESITAINRRWERLKRKLHLNNE"
     gene            8057..9481
                     /locus_tag="PXA33_001670"
     CDS             8057..9481
                     /locus_tag="PXA33_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5120985.1"
                     /translation="MKINVIKPQEEPCSACGKISRIWRRYNGEGYCANCYAKWFPKKE
                     CSCCHQPKRIYLSSTVCWDCEKQTDCVRCGKAAGTFKIGKITRYGAVCNVCSRYFREE
                     RECFECGQLSRTMLRASESGHSELLCLKCFQKYRFATCKNCRRYRKVHDRDNQLCRKC
                     SEQRTSQCPKCKQDMPAGYGNICADCSRRTLLFNLIRMNVHLFRHKKVKRAYKKFIFW
                     YLAKCGVSVVLKKSDDHVLFFMRCDENWGNIPDYRELVTFFKPNGLRTHLTVLRWLLD
                     TNQVKVDESLKEEMAGLERIQSLFNKLSNSVPCIANYYTQLQHKYEAGKTSLKSVRLA
                     LQPAIDFISSESVSLLPTQVQLNSYLVAKTGQIAAITGFINHLNREYHCELEIDRSLI
                     QKMKEKQLKQQAKQRLAILYKQEKLSIKEQNELLSVVLLNLHNIWIKSPKKLSISEID
                     GIAYYVDNEKKYFIPKDIHIRLME"
     gene            9596..9802
                     /locus_tag="PXA33_001671"
     CDS             9596..9802
                     /locus_tag="PXA33_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA delta(2)-isopentenylpyrophosphate
                     transferase"
                     /protein_id="HDL5120986.1"
                     /translation="MTLKAQFREWLKKEQEHDEMIGFAHIPCILSKVDSKLAIVSGNA
                     YEKKYLRYWIKCFRFDRNISKYIK"
BASE COUNT         3032 a         1984 c         2117 g         2684 t
ORIGIN      
        1 agaaattttc acgaagtttt acaaaaacat tccaattttc tcctaaaaaa cttcccacaa
       61 taatttcact ttttgctaca atcctaacca tttatcgaat ttatcaatca gtagagaaga
      121 atatgaatac cagttctttt aatcgcactt gggcaaaagt gattttaagt gcattaatgc
      181 gttatggcgt aaaacatttt tgtattgctc cgggttctcg ctcaacaccg ctcacattag
      241 cggcagtgca acttcaacaa acgcaatatg cagagtgcca cactcatttt gatgagcgtg
      301 gtttaggttt ttatgcgtta gggattgcta aagcaaccga cagcccggtt gctattattg
      361 tcacttccgg cacagcagta gcgaaccttt atcctgcggt gatagaggca agcctgagcc
      421 accaaaaact gattattctt tctgccgacc gcccaaccga attaatcggc tgtggggtaa
      481 accaagccat tcatcagcaa ggtatttttg ccaattaccc ggttgccagt gtcaatctac
      541 cgaaagcgaa cgaggcttac cacgcaagct ggctggtttc gaccattgag caagcctgca
      601 ctacccaagc acaacaaggc ggtgtgttgc atatcaatgt gccttttgaa gagccgcttt
      661 acgaagcaga cattaatgaa attctaaata caccttggtt acgcccaatc caaggctggt
      721 tgaataacgc tcaaaccaaa tggatagacc aacaagcgat tcaaagcgaa gtctcaatgc
      781 acgaaaattg ggattactgg cgaactaaac gaggggtgat tgtggtcggc aaactgccgc
      841 cggaacaagg cacaggttta aaaacttggg cggaaaccat gggttggtgc ttgctaagtg
      901 atgtgcaatc ttgcattgaa gccagcctgc cgtatgccga tatttggctc tccaacgaga
      961 ctgtcaaaca gcgtttattg caagcggata tcgtgattca attcggcacg caaattgtca
     1021 gcaaacgggt gaatgaattt ttaaaagcat tccaagggga atattgggtg gtggaacaat
     1081 cgcacgatta ccataatcca aacgctcatc atcaaacccg ttttattgcc aaagcacatc
     1141 actttttgcg ggtacaccca ccacttcgcc aaaagccatg gttgcttgag ccattggcat
     1201 tatcgcaatt ctgctccact ttcatagagc aacaagtggg tggcagctta agtgaagcct
     1261 cacttgcaca caacattcaa gatgttttag caattaatgg caacttattt atcggcaata
     1321 gcttatttgt tcgattggtt gatgctctgt gcaaactgcc ggaaggctac cctgtttaca
     1381 ccaaccgtgg tgcaagcggt attgatggct taatcgcaac aatggcaggc attgcaaaag
     1441 gtagcgaccg cccgactgtc ggtgtggtag gtgatatttc cgcattacac gatcttaact
     1501 caattgcatt gctcaccaaa attaaccagc cgaccatttt atttgtggtg aataacagcg
     1561 gtggtgcgtt gtttgatatg ttgcctgttg atagcaaagc gaaagatcaa tattaccgcc
     1621 ttgctcacaa ctttgaattt gctcaagtcg ccacaatgtt cgggcttgaa tatatccgcc
     1681 cgtttacttg ggcagatcta aaaaccaaac tcaaacacgc ctatgctcgt aaaggtgtta
     1741 ccattgtgga aatcaaagta aatgaacacg acggcagcaa tatgtataaa tcattgctaa
     1801 aacaaatttc acaggcggaa attgcctaat cacaactgcc cctcgccaat cctgcgaggg
     1861 gtaaaaatgt atcgactatg ctttcctatc aatggcacgc tcaacaaggc acacccgtgg
     1921 ttttccttca cggcttgctc ggctcacaac aagactggca agctgttctc caacatttgc
     1981 aaaattttcc gcaaattcga ccgcttacca ttgaccttcc ttttcacgga aatagtaagc
     2041 acattacctg tgccgatttt gccaacctgc gacaacaact acacgccacc cttgaaagcc
     2101 taatcggctc acgacctttc tatttagtcg gctactcact cggtggacga gcggcactcg
     2161 attatgtgtt aaatatggag aatccgaatc tgcagggcgt ggtgttagaa ggggcgaata
     2221 tcggcttaag cacagaaaac gaccgccaga ttcgccggca aaatgaccgc cattgggcaa
     2281 accgtttccg tcaagaaccc atcgaacaag tgcttgaaga ttggtacaaa cagcctgttt
     2341 ttgcggattt aagcgatacg gaaagagaat gttatattga tagcagaaag cacaatcaag
     2401 gagagcaaat tgctcaaatg ctggaagcca ccagcctcgc caaacagcct gactacaccg
     2461 aaaaactcaa gcagacggat aagaatattg tcttttttat cggtgaaaag gatcagaaat
     2521 ttcgcaaaat ggcatcaagc caccaactac caacacaact gattgctaat gcagggcata
     2581 acgctcatcg ggctaatgct aaaaattttg tggagaaatt ggggttattg atactccact
     2641 ccgacagcga cctacggtcg caatcggtta agcataacgg tgcagctctg ctgcacaaaa
     2701 gccccagtgg ggcttaatca gccgtaggct aaatataacc tagtacggct cagccgtgct
     2761 aggtgttagg gctccccctg caaccgttgc aataagcgat ccatcgcccg ataaccaagt
     2821 gcttccgcta aatgcagttg ttgaatattc gcctcgccgg ctaaatcagc aatagtccga
     2881 gaaactttca aaatacggtg gtaggctcgt accgataaac cgagctttgt caatgcagtt
     2941 tctaaaaata gggcatcttg tgccgataat tggcaatctc gctcaatttc tcgggtggtg
     3001 agttgagaat tgattttacc tgctcgctca aattgcattt ttctggctaa aaacactcgc
     3061 ttgcgaacct gctcggtggt ctctcctcga ttctccgtcc cactttgcaa tgctccttgt
     3121 ggcaaaagtg gcacttctat agagagatcg aaacggtcta agaaaggtcc ggaaaggcgg
     3181 tttaaatacc gcattacctg ttggggcgaa gtgcggttat gcgtgccttg ataatgccct
     3241 gtcgggcttg ggttcatcgc tgcaattaat tgaaagcgtg ccggaaattg tactttagcg
     3301 ttggctcggg aaatcacaat ttcgcctgcc tccaacggct gacgtaacgc atcaagcact
     3361 ttacgttcaa attcaggcaa ttcatctaag aacagcacac cgttatgggc aagtgaaatt
     3421 tcccccggtt tggggatcga gccactgtaa aaactagaaa taaatgtcat aaaaactaga
     3481 aatagctgtc agattattta gaaaacaaga aataggtgtc aaattattat gagaaaaaca
     3541 catagttttc aacaggttag aaaaattaaa cccacttgga tgagtgtatc ggggcatatt
     3601 ccatttagaa acggagtcag tattccctat gaatcgactt tagagcgtga ttttttgatg
     3661 tatttcactt atctaccttc agtagatgaa gtcgtctctc aaccgactac cctacctttc
     3721 gtcaagaatg ggatcactta tacctataca ccagatttct tcttgtcatt tacagacggt
     3781 cgaaagccga tgttaatcga ggtcaaaccg aaagcaaaat ggcagaagca ctggaaagaa
     3841 tggaaagaaa aatggaaagc tgcaatacgt ttttgccaag aaaacggtta tgtattccat
     3901 gtgtatgatg aagatcggat tcgacatcta gcgcttttca atctcaacta tgttcagcgc
     3961 tataagcgaa tacaacatga acaagaagat atagatgcta ttctagccca agtaaagctg
     4021 atgggaaata ccactattga ctatttactc tctcgatttt ttgcaggttc tctatatcgc
     4081 atgaaaggcc tacaaatcat ctatcactta ctggcaacga aacaactaca ttgcaattgg
     4141 tttctaccac ttaatgaatt taccgaggta tgggggaata atgatgagta actctggatt
     4201 tacagaaaag cgctatcatc accgattaga tcggggacgt attattttgc aaaaaggtaa
     4261 tatttaccta aatagagaag atggagaaca gtatgaattg gttgattata tggatgaacc
     4321 ctcccaactg ctcgtacgaa atctcaacac tcgtacaacc aaagtcgtca gtattcatca
     4381 gcttgaaaat ttcaaaatga acgagcgaac ggatctttct gtggatgtaa ccgctattag
     4441 taatgaatat tgggagaaag cccaacaaaa atatgaggca attaaaccgt tattagggat
     4501 ggatcaacat cggccttacg cagttaaggc tcgtgcagaa gatgttgggg tgaaccctcg
     4561 tacattatac cgttggttac aagcctataa ttccatagga tctatcgcag gtttagtcga
     4621 tcaaaaacgt ggatggcaac aaggaaattc ccgtttaacg ccagagcaag acaagctgat
     4681 cgtgcaggta atcaatgagt tttacctaca taaacaacgc ccaactactg agcaaactat
     4741 tagagaaatt cgccgccgat gcaaaattga aaaagtggaa agcccaagca aagaaaccat
     4801 ccgtatgcgc attttgcata tttcagagga agagcgatta cggaaacgcg ggcaacgaga
     4861 aaaggcacgc aacaaattta agccgaagcc aaatagcttt cctgatgcgg attatccatt
     4921 gagtgtggta caaattgacc atacaccagt tgacctaatt attgtggata gtaagtatcg
     4981 taaaccgatt ggccgaccgt ttttgacggt tgcgatagat atatacagca gaatgattgt
     5041 tggttattac ctttcacttg atgcgccttc tgtaacttcg gtggcgatgt gtattgcacg
     5101 tggtattttg ccgaaagaac gcctactgct tgatttaggt ttgcaaggct cagaatggaa
     5161 tgcttttggc tacccagtga aagtgcatgt ggataatggc ccagattttc aggctttgga
     5221 tttatctaaa tcttgttctg cacacggaat ccatcttgaa tttagaccga tggggcgacc
     5281 tgaatatggc ggacatattg agcgtgttat cggtacattt atgaaagaag ttcacagttt
     5341 agcaggaaca acattctcca acatcaaaga gcgtgactct tacgattcgg aaaaagaggc
     5401 cattatgact ctcgatgagt ttgaaaaatg gttggtacat tacattgtga atgtttacca
     5461 caaacgtgtg cattctgctt taggcatttc tcctgaacaa aaatggaaga ttggtatttt
     5521 tggagatgaa aatgaagtgg gatgtggtta tccgcaactt cctgttgatg aacaaacact
     5581 actacttgat ttcttgccaa gtatcactcg taccattcag cataatggtg taacaattga
     5641 cggacttcgt tattacgatg ttgcgctaaa tatgtatatc agtgattcag atgaaagcgg
     5701 taaatctaag gaatttctgt ttcgccgaga cccgcgaaat atcagcaaaa tttggtttta
     5761 tgatccgaaa ttaaaacgtt acttcccaat tccatttgca aatcaggcaa tgcccgaaat
     5821 gagcatttgg gaatatagag aggttcgtag ccgaattgcg aataaaggtg ataaatatat
     5881 caacgagcaa caagttttag acggtttaac cgagatgcgt gaaatggttg ctgaatcggc
     5941 tcaacggacg aaaaaagcac gccgtcaggc tgaacgacaa aaaatgcaca aagcaagtaa
     6001 gccaattatt gaaacaaaag tggaaactaa ggctgttgta ccagtcgtag taacatcgaa
     6061 tttgcttgct ttagatgatg agagcttatc ttttggtgag gtggactaat gcaatacgaa
     6121 catgttcatg aaaaattccg ccatcttgtg acagcggaca atcaggagag gattgcgttc
     6181 ttggacgagc caagatggtt aggttatggt gtcgccaaag acattatgga taatttggtt
     6241 tctttaatga ataaaccaaa acgcccacga atgttgaatt tgttgattgt cggcgattcc
     6301 aataatggaa aaaccacact gatccgccgc ttcttcgact tgtacggtca ggcgtatatc
     6361 gacagtgatt ctaatgcgat ttaccctatt cttcttgccg aagccccacc aagtgcgaat
     6421 gaaaaagaat tgtacatctc attattagag cggttctacg taccatataa gccgacagat
     6481 acgatagcca aacttcgtta tcagactatc catttgttcc gagaatttcg tgtcaaaatg
     6541 ttggttattg acgagtttca ttctctgttg gttggtacac cacgcttaca acgacaggtg
     6601 atgaacgcaa ttaagatgct ttgtaacgag ctacaaattc caattgtcgg tgttggtact
     6661 cgtgatgcaa ttcgggtatt acatacggat cgacaacatg ccagtcgttt tgatgttgcg
     6721 gaattgccaa tatggaaact agataaggaa tttcaaaaac tcctatttca attccaaggg
     6781 attttacctt tgagggagag ttcaaatctg caatctccag aacttgctac gaagatccac
     6841 accatttcag gtgggaactt agggaacgtg catcgcttac taacagcttg tgcagttcaa
     6901 gccattacaa gtggcacgga aaaaatcacc ttagacatca ttgagaaaaa ttcatgggta
     6961 caaccaacaa acggctatcg caaaataatc ggataaaaaa aggtgttatt cataccccta
     7021 tttattcaaa tgaaagtatt agctcgtggt taattcgtgc atcctttgat tgtggtacag
     7081 atccactgac ttttacaggg ttttattggg aaaaacatcg gttgtggaca ttagatttag
     7141 atcgtggctt ggaaaatatt gagccaacta tctatgatga tgtacaggca ttatcgggta
     7201 aaatccaact ttcagaatat tccttgtttc gcaggcttta ccctataaat gcagatgaaa
     7261 ccttagctaa aggtcaagta agatgggtga ttccacgttc ggcaaggaat agaacccatc
     7321 acattggtca acaatattgt cctttgtgct tggatgagtc gccacattta cgcaatgatt
     7381 ggcggttagc gtggaatttt ggttgtctca agcataatat gatgttggcg aacagatgtt
     7441 tttgttgtgg tacattgtat caaccccatt tactatcagc ggaaaagagg catttgaatc
     7501 attgtcatca atgtggtggg aaactggaac aaggcacaat ggaaatgagt ctagctgaaa
     7561 agctgacatt gcaaggtatt aatcaggtac tcgaaaaaga ttcaggcatc tgttttggat
     7621 acaaggtgtc gggtaaggtg tggtttgata tgctccgcta ctgcatcaac ttattgagac
     7681 gtgcatcaga cagaccaaat catgccttag caaaatttgt agttaatcta cagattgatg
     7741 ttgaaaatat atgtagcccc aaaacaggac tttcttttga acaacttccg ctaggtgaac
     7801 gtacaaattt acttgtgaat gcctatcata ttttgggaat ggaagcagat tctattcgta
     7861 gtgcaattta tttgtgtggc ggaacacaaa aaacctttat gtttgaaaaa tatcctgatt
     7921 tttggcaaga atttatggca ctagcacccg caggcccaaa tagaacacga aaacgcacca
     7981 agccccaaaa tgagtctatc acagcaatta atcgccgttg ggagcgtttg aaacgcaaac
     8041 tacacttaaa taacgaatga aaattaatgt aataaaacca caggaagaac cttgctccgc
     8101 ttgtggcaaa atttctcgca tttggcgcag atataatggc gaaggctact gtgccaattg
     8161 ctatgctaag tggtttccga aaaaagagtg cagctgttgc catcaaccaa agcgtatcta
     8221 tttgagttca acagtatgtt gggattgcga aaaacagact gactgtgtac gttgcggtaa
     8281 ggctgcgggt acattcaaaa ttggtaaaat tacccgttat ggtgcagttt gtaatgtgtg
     8341 ttcacgttat tttcgtgaag aacgagaatg ttttgagtgt ggtcaactat ctagaactat
     8401 gcttcgagca tcagagtcag gtcatagcga gctactctgc ttgaagtgtt ttcaaaaata
     8461 tcggtttgcg acttgtaaaa actgtcgccg ttatcgcaaa gttcatgacc gtgataatca
     8521 gctttgtcga aaatgtagtg agcaacgaac ttcacaatgc ccgaagtgca aacaggatat
     8581 gcctgcggga tatggtaata tttgtgcaga ttgttctagg cgaactctac tgttcaacct
     8641 tattcgaatg aatgtgcatt tattccgcca caaaaaagtg aaaagagcat ataaaaagtt
     8701 cattttttgg tatctcgcaa agtgtggcgt gagtgttgtg ctgaaaaaat cagacgacca
     8761 cgtcctcttt tttatgcgtt gcgatgaaaa ttggggcaac attccagact accgtgagtt
     8821 ggttacattc tttaaaccca atggattacg gacgcatttg actgtgttgc gttggttgct
     8881 tgataccaat caagtcaaag tagatgagag cttaaaagag gaaatggcgg ggttggaacg
     8941 tattcaatcc ctatttaaca aattaagtaa ctctgtacct tgtattgcaa actactacac
     9001 gcagttacaa cataaatatg aggcgggcaa aacatcactg aaatctgttc gattagcttt
     9061 acaacctgcg attgatttca tcagttcaga atctgtctcg ttactgccga ctcaagtaca
     9121 actgaatagc tatcttgtgg caaaaacggg acagattgct gctattacag gctttataaa
     9181 ccacctgaac cgagaatatc attgtgaatt agaaattgac cgctccctca ttcaaaagat
     9241 gaaagaaaag cagttgaagc agcaagcaaa acagcgatta gcgatactct acaagcaaga
     9301 aaaactaagt atcaaagagc aaaatgagct actctcagtc gtattactga atttgcacaa
     9361 catttggata aaatcaccga aaaaattaag tatttcagag atagacggta tagcctacta
     9421 tgttgataat gagaaaaaat actttatacc aaaagacatt cacatacgcc tgatggaata
     9481 agctgtcgtc aatttttatt tttgcgatct gtgtcgagaa aataatattt atgaatcgac
     9541 agatgaaata atattgataa agtctctttg tgtgttcaac aaacaggaga taaatatgac
     9601 attaaaggct caatttcgtg aatggcttaa aaaagagcaa gaacatgatg aaatgatagg
     9661 gttcgcccat atcccttgca ttttatctaa agtggatagc aaactggcga ttgttagcgg
     9721 aaatgcttat gagaagaaat atctcagata ttggatcaag tgttttaggt tcgatagaaa
     9781 tatcagcaag tatattaaat aggttaaata tctagct
//
LOCUS       DANLRK010000095         9804 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.095, whole genome shotgun sequence.
ACCESSION   DANLRK010000095 DANLRK010000000
VERSION     DANLRK010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9804
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.095"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(115..1830)
                     /gene="proS"
                     /locus_tag="PXA33_001672"
     CDS             complement(115..1830)
                     /gene="proS"
                     /locus_tag="PXA33_001672"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDL5120987.1"
                     /translation="MRTSQYLFSTLKEMPNDAQVVSHQLMLRAGMIRPMASGLYNWLP
                     TGIKVLKKVENIIREEMNKGGAIEVLMPVVQPAELWVESGRWNDYGPELLRFKDRGER
                     DFVLGPTHEEVITDLVRREVSSYKQLPLNLYQIQTKFRDEVRPRFGVMRSREFVMKDA
                     YSFHTTQESLQETYDVMYQVYSNIFTRLGLDFRAVQADTGSIGGSASHEFQVLASSGE
                     DDVVFSTESDFAANIELAEAVAVGERQAPTAEMQLVDTPNAKTINELVENHGLAIEKT
                     VKTLVVKGATEEQPLIALVIRGDHELNEIKAQKHPLVADPLEFADEAEIKAKIGAGVG
                     SLGVVNMPIPVIIDRSVALMSDFGCGANIDGKHYFNVNWERDVAVPEIADLRNVVEGD
                     PSPDGKGTLQIKRGIEVGHIFQLGKKYSEAMKATVQGEDGKPLVMTMGCYGIGVTRVV
                     AAAIEQHYDDRGIIWPCDEMAPFTVAIVPMNMHKSEKVQAFAEELYANLKAQGVEVIF
                     DDRKERPGVMFADMELIGVPHMVVIGEKNLENGEIEYKNRRSGEKQMIAKESLLAFLK
                     ANVKA"
     gene            complement(1994..2155)
                     /locus_tag="PXA33_001673"
     CDS             complement(1994..2155)
                     /locus_tag="PXA33_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5120988.1"
                     /translation="MEQKLNNVFGGIMFKEKDYDEYLEAKVKKGQIFTLEQANAEWQE
                     IIEQIEREN"
     gene            complement(2208..4091)
                     /gene="htpG"
                     /locus_tag="PXA33_001674"
     CDS             complement(2208..4091)
                     /gene="htpG"
                     /locus_tag="PXA33_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HDL5120989.1"
                     /translation="MANNQETRGFQSEVKQLLQLMIHSLYSNKEIFLRELISNASDAA
                     DKLRFKALSEPALYEGDGELRVRVSVDETLGTITIADNGIGMTREQVIDHLGTIAKSG
                     TKEFLNALGTDQAKDSQLIGQFGVGFYSSFIVADKVTVKTRAAGVPAEQGVIWESAGE
                     GDYTVADLEKKERGTEIILHLREDEKEFLNEWRLREIIGKYSDHIGLAVEIQTNEYDD
                     EGKASGQKWEKINKAQALWTRSKNEISDEEYTEFYKHLSHDFADPLIWSHNKVEGKQE
                     YTSLLYVPSKAPWDLFQREQKHGLKLYVQRVFIMDDAEVFMPNYLRFMRGLLDTNDLP
                     LNVSREILQENKVTQSLRSALTKRALQLLEKLAKDDAEKYAQFWQAFGVVLKEGVGED
                     FANKQQVAGLLRFASTQRDDSEQAVSFADYIARMKEGQKAIYFLTADSYQAAKNSPHL
                     ELFHKKGIEVLLLSDRIDEWLVSHLTEFDGKPLQSITKSDLDLGELADKQEEESQKAQ
                     QAEFGSFLERAKGYFGERVKNVVLTHRLTDTPAVVSTDSDEMTTQMAKLFAAMGQQAP
                     EVKYTFELNPDHPMVKKVADLADEAEFADWIELLFEQALLAERGTLENPTAFIKRVNK
                     LLG"
     gene            4255..4542
                     /locus_tag="PXA33_001675"
     CDS             4255..4542
                     /locus_tag="PXA33_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HDL5120990.1"
                     /translation="MAITITSREFNQNASKVLKMAEKEPVFITKWGKIVNVVSSYMDY
                     QQKKPSEPSFEELFASSENEDVSDEFWQEFEQTLVEIRKSNKPRPVDFGDE"
     gene            4542..4970
                     /locus_tag="PXA33_001676"
     CDS             4542..4970
                     /locus_tag="PXA33_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HDL5120991.1"
                     /translation="MYRYLFDTNIVSELYKLRNDKMDVNVRQWLRGVNPSQTNISCIT
                     LSEIKTGILLKARKDKEQSDRLNDWFENNVLKAYQTKAFVVNNDIALLASEYHIPNKM
                     DLNDAYIAATAKYHNLILVTRNTKDFIRCDVRLFNPFETA"
     gene            4974..7055
                     /gene="recG"
                     /locus_tag="PXA33_001677"
     CDS             4974..7055
                     /gene="recG"
                     /locus_tag="PXA33_001677"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HDL5120992.1"
                     /translation="MSEQLLDGVPLTALSGVGAAISEKLSRIGINNVQDLLFHLPMRY
                     EDRTRITPIADVRPESFATIEGYVQLTEVQFGKRPILSVTLSDSTSKIMLKFFNFNAG
                     MKNSLATGVRVKAFGEIKRGRFMAEIHHPEYQIIRDNQPLVLAETLTPIYSTTEGLKQ
                     ASLRKLTEQALALLEKVKVAELLPDEYNPHKYSLKEALQLLHRPPPTISAEVLEKGDH
                     PAQKRLIFEELLAHNLAMQQLRLGVKQQYAEALTYQTDIKQRFLDSLPFKPTHAQSRV
                     TADIEQDLAKPHPMMRLVQGDVGSGKTLVAALAALLAIDNGKQVALMAPTEILAEQHA
                     NNFANWLNPFGIEVGWLAGKVKGKARTAQLEAIKNGDVQMIIGTHALFQDNVEFHHLA
                     LVIIDEQHRFGVHQRLTLREKGAKENNYPHQLIMTATPIPRTLAMTVYADLDTSIIDE
                     LPPGRTPITTVVISEDRRAEIVQRVYQVCKQEHRQAYWVCTLIDESEVLEAQAAAAIA
                     EDLQRALPDLRIGLVHGRLKPQEKQAIMAEFKAANLDLLVATTVIEVGVDVPNASLMI
                     IENSERLGLAQLHQLRGRVGRGATASHCVLMYKPPLGKISSKRLQVMRDSQDGFYIAE
                     KDLEIRGTGEILGTKQTGMAEFKVADLMRDRKMIPLVQRYAKQIISENQPLAEALIKR
                     WLDNKAEYTNA"
     gene            complement(7077..8011)
                     /locus_tag="PXA33_001678"
                     /pseudo
     CDS             complement(7077..8011)
                     /locus_tag="PXA33_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250489.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
     gene            complement(8064..9716)
                     /locus_tag="PXA33_001679"
     CDS             complement(8064..9716)
                     /locus_tag="PXA33_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HDL5120993.1"
                     /translation="MTIFQVAQNWLAQDPDAETRAELEQLIQAAESDEKAKAELTARF
                     DGRLQFGTAGLRGRLQAGSMGMNRVLVAQAASGLAGFIKGYDKEPSIVIGYDGRKNSD
                     VFARDTAEIMAAAGIKTYLLPRKLPTPVLAYAIQYFDTTAGVMVTASHNPPEDNGYKV
                     YLGKANGGGQIVSPADKEIAALIDKVAAGDIRDLPRSQDFTVLDDEVVNAYIEKTASL
                     AKRPKAEINYVYTAMHGVGYEVLSKTLEKAGLPQPYLVSEQIQPDGSFPTVNFPNPEE
                     KGALDLAIKLAKEKNAEFIIANDPDADRLAVAVPDAQGNWKPLHGNVIGCFLGWYLAK
                     QFHAQGKQGVLACSLVSSPALAEIAKKYGLSSEETLTGFKYIGKVENLLFGFEEALGY
                     LVDPDKVRDKDGISAAIMFLDLVCSLKQEGKTLADYTTEFVQEFGAYVSGQISIRVCD
                     LAEIGKLMTALRNNPPSEIGGFKVAEFIDHTKTTRQNDILVFVLENGSRLIARPSGTE
                     PKIKFYLDARGTDAENAEQVLAQFDESVRSLLRQEEYGKQDC"
BASE COUNT         2631 a         2357 c         2066 g         2750 t
ORIGIN      
        1 cacgcattct atttcctata ttctaaaaat aatagagtat tttatacttt agttaaatac
       61 tcgctgcttt atattaaagg gcgtatttta tacgcccaat tttgttattt ataattaagc
      121 ctttacattc gcttttaaga acgcaagtaa gctctctttg gcgatcatct gtttttcacc
      181 gctgcggcgg tttttgtatt cgatttcgcc gttctcgagg tttttctcac caataaccac
      241 catatgcggc acaccgataa gctccatatc cgcgaacatc acgcccgggc gttctttacg
      301 atcatcaaaa atcacctcaa cgccttgagc ttttaagtta gcgtataact cttcagcgaa
      361 tgcctgcact ttttccgatt tgtgcatatt catcggcaca atcgccaccg taaatggcgc
      421 catttcatca caaggccaga taataccacg atcatcataa tgttgttcaa tcgcagctgc
      481 caccacacgg gtaacgccga taccgtaaca acccattgtc atcactagcg gtttaccgtc
      541 ttcaccttgc acagtggctt tcattgcttc tgagtatttt ttgcctaatt ggaaaatatg
      601 ccctacttca atacctcgtt tgatttgaag tgtgccttta ccgtccgggc ttgggtcgcc
      661 ttccaccaca ttgcgtaaat ctgcaatttc cggcacagca acatcacgct cccagttcac
      721 gttaaagtag tgtttgccat caatattcgc accgcagcca aaatctgaca tcaacgccac
      781 tgagcggtca ataatcaccg gaatcggcat attcaccaca cctaacgagc caacaccggc
      841 accgattttg gctttaattt ccgcttcatc ggcaaactca agcggatcag ccaccaacgg
      901 gtgtttttga gctttaattt cgtttaactc gtggtcgcca cgaatcacta acgcaattaa
      961 cggttgctct tcagttgccc ctttcaccac taaggttttc accgtttttt caatggctaa
     1021 accgtgattt tctactaatt cattgatggt tttcgcattt ggcgtatcca ctaattgcat
     1081 ttctgccgtt ggtgcttggc gttcacccac cgcaaccgct tcggctaatt caatattggc
     1141 agcaaaatct gattcggttg aaaataccac atcatcttca ccgcttgagg ctaacacttg
     1201 gaactcgtga gaggctgaac cgccgataga gccggtatct gcctgcaccg cacggaaatc
     1261 taagcccaaa cgggtgaaaa tattgctgta aacctgatac atcacatcat aggtttcttg
     1321 caggctctct tgtgtggtgt ggaaagaata ggcatctttc atcacaaatt cacgcgaacg
     1381 cattaccccg aaacgagggc ggacttcatc acggaatttc gtttgaattt ggtataaatt
     1441 cagcggaagt tgtttgtacg aagaaacttc acggcgaacc aagtcggtaa tcacttcttc
     1501 gtgagtcggg ccaagcacga aatcacgctc gccacgatct ttaaaacgta ataattccgg
     1561 gccgtaatca ttccaacgac cggattccac ccataattcc gccggctgaa ccaccggcat
     1621 taatacctcg atagccccgc ctttgttcat ctcttcacga ataatatttt ccactttttt
     1681 caacactttg atcccggtcg gcaaccagtt gtataaacct gacgccatcg ggcgaatcat
     1741 tccggcacgc aacattaatt gatggctaac tacttgtgca tcattcggca tttctttaag
     1801 ggttgaaaat aaatattgac ttgtacgcat tgctattcct attcttttta aactttgaaa
     1861 cctgaaaaaa tctgcttatt ttaaaacaaa gcggttaaat tttcaggaaa ttttgcaaaa
     1921 tcctataaaa aaataaccgc ttgtaagcgg ttattttgtt ttaactaagg gtattttctt
     1981 aaactgtgta tgcttaattt tctcgttcaa tttgctcaat aatctcttgc cattcggcat
     2041 ttgcttgttc aagagtaaaa atctgacctt tttttacttt agcctctaaa tattcatcat
     2101 aatctttttc tttaaacatt attcccccga atacattgtt taatttttgt tccattatat
     2161 attgaatatg gaaaaacgga aacctagatt tccgttttta gtaatggcta gcccaacaat
     2221 ttatttaccc gtttaataaa ggctgtcggg ttttccaacg tgcctcgttc agcaagcaga
     2281 gcttgttcaa acagcagttc aatccaatcg gcgaactcgg cttcatcggc aagatcagct
     2341 acttttttca ccattgggtg atctgggttc agctcaaaag tatatttcac ttctggtgct
     2401 tgttgcccca ttgctgcaaa cagttttgcc atttgggtgg tcatttcatc gctgtcagtg
     2461 gaaaccactg ccggtgtgtc ggttaagcgg tgagtcagca ccacgttttt gacccgttca
     2521 ccaaagtagc ctttcgcccg ttctaagaac gaaccaaact ccgcctgttg ggctttttgg
     2581 ctctcttctt cctgcttgtc ggcaagctcg cctaagtcta agtccgactt ggtaatgctt
     2641 tgtagcggtt tgccgtcaaa ctcggtgagg tggctaacca accattcatc aatgcggtca
     2701 gaaagcagta gcacctcaat gccttttttg tggaacagct ctaagtgcgg gctgttttta
     2761 gccgcttggt agctgtctgc ggttaagaag tagatcgctt tctgcccctc tttcattctt
     2821 gcaatataat cggcaaaact gaccgcttgt tcgctgtcat cacgctgggt ggaggcaaaa
     2881 cggagtaagc ctgcaacctg ttgtttgttg gcaaagtctt cgcccacgcc ctctttaagc
     2941 accacgccga acgcttgcca gaattgggca tatttttcgg catcgtcttt cgccaatttt
     3001 tccagcagtt gtaacgcacg cttggttaag gcagaacgga gcgattgggt aactttattc
     3061 tcttgcagaa tttcacggct gacgttgagc ggcaggtcgt tggtgtcgag caagccacgc
     3121 ataaaacgca agtagttcgg cataaagact tccgcatcgt ccataataaa tacacgctga
     3181 acgtagagtt tcaagccgtg tttttgctcg cgttggaaca gatcccacgg ggctttgctc
     3241 ggcacataca gcaagctggt gtattcttgc ttaccttcga ctttattgtg cgaccaaatc
     3301 agcgggtcgg caaaatcatg gctgaggtgt ttgtaaaatt cggtgtactc ctcgtcacta
     3361 atctcatttt ttgagcgtgt ccaaagcgct tgagctttgt taattttttc ccatttttga
     3421 ccgcttgctt tgccctcgtc atcgtattca ttggtctgaa tttccaccgc caggccgata
     3481 tggtcggagt atttgccgat aatctcacgc aaacgccatt cgtttaaaaa ctctttttcg
     3541 tcctcacgca agtgaagaat gatttccgtg ccacgctctt ttttctccag atcggcaacg
     3601 gtgtaatcgc cctcaccggc agattcccaa atcacgcctt gctcggcagg cactccggcg
     3661 gcacgggttt tgactgtcac tttatccgcc acgatgaaag aagagtaaaa gcccacgccg
     3721 aactgcccga taagctggct gtctttggct tgatcagtgc cgagtgcgtt caaaaattct
     3781 ttggtaccag attttgcaat cgtaccgaga tggtcgatca cctgctcacg ggtcataccg
     3841 ataccgttat cggcaatcgt aatcgtaccg agtgtttcat caacagagac acgcacacgc
     3901 agttcgccat caccttcata caaagccggc tcggaaaggg ctttaaaccg caatttatcg
     3961 gcggcgtcag aggcgttgga aataagctca cgcaggaaaa tttctttgtt ggagtaaagc
     4021 gagtggatca tcagttgcaa taactgtttc acttccgact ggaagccacg ggtttcttga
     4081 ttatttgcca ttgtgtattc ctttattcat taaaaaacag ggaatagatg gggcaagtgg
     4141 tcagattttc aagatttttt gcaaaatatc tgttgccaaa tagcgaatta attgaaagat
     4201 attactacat ttaataaaat acactcatct ttttactaca ttttggagag cgatatggca
     4261 ataaccatta cttccagaga atttaatcaa aatgccagca aagtgttaaa aatggcagaa
     4321 aaagagccgg tttttattac caaatggggc aaaatcgtca atgtggtgtc tagttatatg
     4381 gattatcagc agaaaaagcc tagcgagcca agctttgaag agctattcgc ctcttccgaa
     4441 aatgaggatg tgagcgatga attttggcaa gagtttgaac aaacattggt tgaaatccgc
     4501 aaaagcaata aaccaagacc tgttgatttt ggagatgagt aatgtatcgc tacctttttg
     4561 ataccaatat agtttccgag ctttataagt taagaaatga caagatggat gtcaatgttc
     4621 gccagtggct gagaggggtt aatcctagcc agaccaatat cagctgtatt acgttatctg
     4681 aaatcaaaac aggtattttg ctgaaagccc gcaaagacaa agaacaatca gaccgattaa
     4741 atgattggtt tgaaaataat gtattaaagg cttatcaaac aaaagcgttt gtggtgaata
     4801 acgacattgc tctattggca tcagagtacc atattcccaa taaaatggat ttaaatgatg
     4861 cttatattgc ggcaaccgcc aaataccaca atctgatttt agtcacccgc aacaccaaag
     4921 attttatccg ttgtgatgtg cgtttattta acccatttga aaccgcataa ataatgagtg
     4981 aacaattatt agacggcgtg ccattaaccg ccctttccgg cgtaggagcg gcaatttccg
     5041 aaaagttaag tcgaatcggc attaataatg tgcaggattt actgtttcat ctgccaatgc
     5101 ggtatgaaga tcgcacccgt attaccccga ttgctgatgt tcgcccggaa agctttgcca
     5161 ccattgaagg ctatgtacaa cttaccgaag tccagtttgg taaacgcccg attttatcgg
     5221 ttacactctc cgacagcacc agcaaaatta tgcttaaatt cttcaatttc aatgctggaa
     5281 tgaaaaatag ccttgccacc ggcgttcggg taaaagcctt tggagaaatt aagcgtgggc
     5341 gatttatggc ggaaattcac caccctgaat atcaaattat tcgggataat caaccgcttg
     5401 tactggctga aaccctgacc ccgatttact ccaccactga ggggttaaaa caagcctcgc
     5461 tgcggaaatt aaccgagcaa gccctcgctt tattagagaa agtgaaagtc gccgagctgt
     5521 tgcctgatga atacaatccg cacaaataca gcctaaaaga agcgttgcag ttattgcacc
     5581 gcccaccgcc gacgatttct gccgaagtct tggaaaaagg tgaccaccct gctcaaaaac
     5641 ggctgatttt tgaagaactg ctggcacaca atttagcgat gcagcaactc cgtttagggg
     5701 taaaacagca atatgcagag gcacttactt accaaaccga tataaaacag cgttttctcg
     5761 acagtttgcc ttttaagcct actcacgcac aaagccgagt aaccgcagat attgagcaag
     5821 atcttgccaa accacacccg atgatgcgac tggtacaagg cgatgttggt tcaggtaaaa
     5881 ccttagtagc cgccttggca gcactattag cgattgataa cggcaagcag gtggcattaa
     5941 tggcaccgac cgaaattctt gccgaacagc acgccaataa ttttgccaac tggcttaatc
     6001 cctttgggat tgaggttggc tggcttgccg gtaaagtgaa aggcaaggcg agaaccgcac
     6061 aacttgaggc aatcaaaaac ggtgatgtgc agatgattat cggcactcac gccctgttcc
     6121 aagacaatgt ggaatttcac catttagcgt tggtgattat tgatgaacaa caccgttttg
     6181 gcgtacacca acggctgacc ttgcgggaaa aaggagcgaa agaaaataac tatccgcatc
     6241 agctcattat gacggcgacc cccattccga gaacgctggc aatgacggtc tatgctgacc
     6301 tcgacacctc aatcattgac gaactgccac cgggtagaac gccaatcacg actgtggtga
     6361 tttctgaaga tcggcgagca gaaatcgtcc aacgggtgta ccaagtctgt aagcaagaac
     6421 accgccaagc ctattgggtc tgcaccctga ttgatgaaag cgaagtgttg gaggctcaag
     6481 ccgcggcagc gattgccgaa gatttacagc gagccttgcc ggatttacgc atcgggttag
     6541 tacacggacg gctgaaacca caggaaaaac aagcgattat ggcggaattt aaggcagcga
     6601 atttagattt gctggttgcg accaccgtaa ttgaagtggg agtggacgtg ccgaatgcta
     6661 gcctaatgat tatcgaaaat tccgaacgac tcgggctggc tcaactccac cagttgcgtg
     6721 gacgtgttgg gcgaggggca accgcctcgc attgcgtctt aatgtacaaa ccgccgctcg
     6781 ggaaaatttc cagcaagcgg ttacaagtga tgcgtgatag ccaagacggt ttctacattg
     6841 ccgaaaaaga tttggagatt cgaggcacag gcgaaattct cggcacaaaa cagaccggaa
     6901 tggcagaatt taaggtggcg gatttaatgc gagaccgcaa aatgattccg ctggtgcaac
     6961 gttacgccaa gcaaattatt agcgaaaatc aaccgcttgc agaggcgtta attaagcgtt
     7021 ggttagacaa caaggcggaa tataccaacg cttaaccggc aagcgggtta tttagattac
     7081 ggcaggtttt tcaccgattc aattaccaaa tcccaaaagc gggatttgtc taattttttt
     7141 gctacccaag tattacactg ttttggcggg tagcggaaat ccgccaccgt catacccagt
     7201 gtatgcgtgc ctgttagctc aatattgacc ggcacttgct gaacttcaat caggcttggg
     7261 tcaatcacat acgccaccgc acaggcatcg tgaaccggtg gagcatcaaa gccttgagcc
     7321 tgtttataca ttttggcgaa gaacgccagc aattccacca caaactgccc cgatttgctg
     7381 tttaatgcct taaagcgttc aaccacatcg ggggtcgcaa gtgcttggtg agttaagtct
     7441 aagccgacca tcgtcacctt ccaaccggca ttaaacacaa tatgagcagc ttccgggtca
     7501 attttaatat taaattctgc caccgcactc cagttgccgg tgtgataacc tccgcccatc
     7561 agcaccactt ctttcactct ttcaataata cggggctcaa ggcgggcagc tagagcaata
     7621 ttggttattg cccaccggtc ggcactaagg taatgctttt ctccgggtgc gacattatca
     7681 aatcaataat taattgagcg gcgtgttttt tctcaaattg tcgggtagat tcagggagta
     7741 caggcccatc cattcccgaa tcaccgtgaa tatccggggc attttccact tcacgcacca
     7801 gcggtctggc acaacctttg gcaaaaggaa cgttgcaaat atgagcaatt tccgcaaccg
     7861 ccaacgcatt acgcgatact ttttctaagg tttgattacc taccaccgta gtcactgcca
     7921 acaaatcaat attcggattc ccccaagcga gcatcatcgc aatcgcatcg tcatgccccg
     7981 gatcacaatc taaaataatt ttttgagcca ttattgtctc ctttaaaaaa gggagagcct
     8041 aagctccccc tcaattaatg ccattagcaa tcttgtttgc cgtattcttc ttggcgaagc
     8101 agcgaacgca cactttcgtc aaactgagcc agcacttgct ctgcgttttc agcgtctgtg
     8161 ccacgagcat ctaaatagaa tttaattttc ggctcagtac ctgaagggcg agcaatcaaa
     8221 cggctgccat tttccaacac aaacactaaa atgtcatttt ggcgtgtggt tttggtatgg
     8281 tcaataaact cagccacttt aaagccacca atttcgcttg gaggattgtt gcgtaacgcc
     8341 gtcattaatt tgccgatttc ggctaaatca caaactctga tcgaaatttg accgcttaca
     8401 taagccccga actcttgtac aaactcggtg gtgtaatccg caagggtttt accttcttgt
     8461 ttgaggctgc acactaaatc taagaacata atggcagcgg aaatgccgtc tttatcacgc
     8521 actttgtctg ggtcaaccaa ataacctaag gcttcttcaa agccaaataa taggttttca
     8581 acttttccga tatatttaaa gccggttaag gtttcttccg agcttaaacc atattttttc
     8641 gcaatttcag ctaacgccgg agaggatact aacgagcaag ccaacacgcc ttgtttacct
     8701 tgtgcgtgga attgtttggc taaataccag cctaagaaac agccgatcac attgccatgc
     8761 agtggtttcc agttgccttg agcatcaggc acggctaccg ctaaacggtc agcatccggg
     8821 tcgttggcga taataaattc ggcatttttc tctttcgcca atttaatcgc taaatctaac
     8881 gcccctttct cttccgggtt cgggaaatta accgttggga acgaaccgtc cggctgaatc
     8941 tgctcactaa ctaaatacgg ttgcggtaag ccggcttttt ctaatgtttt gcttaacact
     9001 tcatagccca caccgtgcat tgcagtgtaa acgtagttta tttcggcttt tggccgtttg
     9061 gctagtgagg cggttttctc aatataagca tttaccactt catcgtctaa caccgtgaaa
     9121 tcttggctac gaggtaaatc acgaatatcg cctgctgcta ctttatcaat cagagcagca
     9181 atttctttat ctgccggcga aacaatttgt ccgccaccgt tggctttacc taaataaact
     9241 ttatagccgt tatcttccgg tgggttgtgg ctggcagtta ccatcacacc ggctgtggta
     9301 tcaaaatatt gaatcgcata ggcaagcact ggtgttggca atttgcgagg aagcaggtac
     9361 gttttaatgc ctgccgctgc cataatttca gcggtgtcac gggcaaacac atcggaattt
     9421 ttacgaccgt cataaccaat aacaattgac ggctctttat cataaccttt aataaaccca
     9481 gctaaaccgc tggcagcttg tgccaccaaa acacggttca tacccattga acccgcttgt
     9541 aagcgaccac gcaaaccagc cgtaccaaac tgtaaacgtc cgtcaaaacg ggctgttaat
     9601 tctgcttttg ctttctcgtc cgattctgcc gcttgaatta actgctctaa ttccgcacgg
     9661 gtctctgcat ccgggtcttg ggctaaccaa ttttgtgcaa cttggaaaat agtcattttt
     9721 tactcctatg taatttaaaa actctgttta gattagccca aagtcctttt atttaaaagg
     9781 agatatttct ttttttgtga tcga
//
LOCUS       DANLRK010000096         9723 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.096, whole genome shotgun sequence.
ACCESSION   DANLRK010000096 DANLRK010000000
VERSION     DANLRK010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9723
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.096"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(<1..268)
                     /locus_tag="PXA33_001680"
     CDS             complement(<1..268)
                     /locus_tag="PXA33_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5120994.1"
                     /translation="MSSIKEIFSTKNSPINVLMQTEWFLGFILIVVYLDAYFQLVHQV
                     PIYIAIQQKEIYLTKTIDYIAAIFFFSVTFTALFTMMRLLHIFIL"
     gene            complement(338..1471)
                     /locus_tag="PXA33_001681"
     CDS             complement(338..1471)
                     /locus_tag="PXA33_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL5120995.1"
                     /translation="MKTIKIGLVGTGYIGRCHAIAYAQAPTLFPLKGKIQLEYLAEIN
                     QALADQKAREFGFQRATDDWRKLVTDPNVDVVDICTPNFLHKEIALEAIKHGKHVYSE
                     KPLALTAQDAKEMVIAAQQAGVKTLVGFNYIKNPTTQLARQIIQNGEIGEVVHFYGTH
                     NEDYLANPNTPIDWHCYKAKAGLGTLGDLAAHIVSIAHYLVGSDIERVIGDMQTVIEE
                     RPSPHNPAEKIKVENEDQASSLIRFANGVMGTLESSRISCGRKMGLTYVVTGTKGTLS
                     YTQERMAELKLYLHDDDKSRQGFKTILVGPEHPDYAAFCVSAGHGIGFNDQKAVEIRD
                     LINGIAADDPMYPDFEEGYKISRVLEAIAKSAEEKRWVEVSEI"
     gene            complement(1602..2630)
                     /locus_tag="PXA33_001682"
     CDS             complement(1602..2630)
                     /locus_tag="PXA33_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL5120996.1"
                     /translation="MTTQFLKNMGMVDDKGKIDFIAFFERFGVLIFLILLIIFFTSQN
                     GAFLSQRNIYNVLTEVSIFGIMAVGMTFVILTAGIDLSVGSILAVTAMFAAYIIKGDN
                     TVTLEAGAWGGMSWLIGLSICLGLGTFIGWLHGLGVTKLKLPPFIITLGGMTIWRGLT
                     LVLNNGSPIAGFDEGYRWWGRGDILGVPVPVVIFAVVAIIGYFALHKTRWGRYVYSVG
                     GNPEAARLAGVNINRTLVSVYVVIGALAGLAGFILSARLGSAESVAGVSFELRVIASV
                     VIGGTSLMGGYGRVTGTIIGSIIMGVLINGLVLMDVSAYYQQIIMGLIIILAVAFDTY
                     AKSRRGAL"
     gene            complement(2642..4186)
                     /locus_tag="PXA33_001683"
     CDS             complement(2642..4186)
                     /locus_tag="PXA33_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDL5120997.1"
                     /translation="MSTPLLEVKDLTKSFSGVMALNGVKLTVGKGEVHALLGENGAGK
                     STLLKALSGAQPQTSGEIIFNGETLSLSDSPFDRQLKGIVTIYQEFNLLPNMTVAENF
                     FLGREPVKGLFVNEKAVNEEAQLVLDNLGLNIKPDTQVSRLSVAQQQMVEIARAMTLN
                     AKLIIMDEPSAALSDKEVETLHQIVRDLKSRGISVIYVTHRLNEVFALCDRFTVFQDG
                     RYSGEGEVKNVNVDQIIKMMVGREVAFTRRPPEETHHTHRPIRLAVKNLHREKPAFDP
                     HGIALHGVSFNIHEGEVLGIAGLVGAGRTEIARCLFGVENYTADEISLDGKPYAATSP
                     LDALEKGVALVPEDRKKEGLVLGLPIKTNMTLPILSKLLKGNLFVDNQKEDDLIETYR
                     QALRIKMASSELEARKLSGGNQQKVILARCMALNPKVLIVDEPTRGIDVGAKSEVHQV
                     LFDMAKQGVAVLVISSDLPEIMAISDRIITLSEGRVTGEIHGDDATEEKLMSMMAVGV
                     QRKAAA"
     gene            complement(4237..5175)
                     /locus_tag="PXA33_001684"
     CDS             complement(4237..5175)
                     /locus_tag="PXA33_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDL5120998.1"
                     /translation="MKKRIKFVLSALTLGLAMNSFAKDELVVFSLPNLSSPFEVQLQK
                     VAVETSKKLEIKLQVLDGQSSSTKQASDLENAITRGAKGIIISPNDVNAISGAVEEII
                     KEKIPAATLDRKVESSKPVPHFGANNYTGGQEVAKAVKAKYPNGAKIILLTGQPGSTS
                     NIERTKGIRDELAAGGDKYKIVVDQTGNWLRSEGLRIIESVLPTLKEKPEVIISANDD
                     MALGAIEALRSQGLKAGDILVTGFDSTPEALARVKDGWLYLTADQRPSFAVSTALEQV
                     VGNIRESKEVSGADYPPKIILKDNLQEAERFAEISE"
     gene            complement(5507..7018)
                     /locus_tag="PXA33_001685"
     CDS             complement(5507..7018)
                     /locus_tag="PXA33_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HDL5120999.1"
                     /translation="MSQFVQNFINGAVVESQSQRVTSVFNPATGEETKKVKLSLPNEV
                     DAAIAAADQAFKSWSQQSPLRRARILFKFKELLEANFDELARLISSEHGKIYSDAIGE
                     LTRGLEVVEFATGIPHLQKGEFSANAGRGIDIHSIQQPLGVVAGITPFNFPAMVPMWM
                     FPVALACGNTFVLKPSEKDPSVSIRLAELLKEAGLPDGVFNVVQGDKEAVDILLTDSR
                     IQAVSFVGSTAIAQYIYEKGSANGKRVQALGGAKNHALIMPDADVQGTINALLGAAFG
                     AAGERCMALSVAVVVGDELADKIIEELIPKVKALKIGSGLLPQGVLENDMGPVISKEH
                     KAKIESYIDQGVAQGAKLCVDGRGYKVEGFENGYFVGGTLFDNVTPEMTIWQDEIFGP
                     VLSVVRVKDYAEGIALINSHQYGNGSAIFTADGDSARQFTQDVQAGMVGVNIPIPVPM
                     AFHCFGGWKASIFGPLNVYGTDGVRFYTRMKTITTRWPDSSVRSQAAFNFPTL"
     gene            7237..8061
                     /gene="iolB"
                     /locus_tag="PXA33_001686"
     CDS             7237..8061
                     /gene="iolB"
                     /locus_tag="PXA33_001686"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HDL5121000.1"
                     /translation="MSSPLLSKPNFNQHEGLIQHITPENAQWEYVGFDVYQLSKQQIT
                     LESGENELCLVFVTGFGSVYVDDYAFENIGNRTTPFEKIPPYAVYIPPHKSVLVKTEL
                     NLELAVCKAPSQGNYPVRLISPNHIGVEKRGFGNNQRLVHNILPETEAADSLLVVEVF
                     TDEGNTSSYPSHKHDDKHSTEETYLEETYYHRFDPPQGFAMQRVYTDDRTLDECMAVY
                     DKNVVKVPRGYHPVATIAGYNNYYLNVMAGPVRKWKFTWEQDHLWVNSETYKAKFE"
     gene            complement(8137..9588)
                     /locus_tag="PXA33_001687"
     CDS             complement(8137..9588)
                     /locus_tag="PXA33_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HDL5121001.1"
                     /translation="MPNHKFLLLRFQAYINGKFVSSQNGKTFTVQNPTNGEKIGEVAD
                     LGTEETAQAIEAAAVAQKKWQALLPKERSVILRKWFELIMQNQEELAQILSLEQGKPL
                     AESRGEIAYGASFIEWFAEESKRIYGDVLPQDKAGRRLFVTKQPIGVVAAITPWNFPN
                     AMITRKAAPALAAGCAIVIKPAAETPLSALALAQLADQAGIPKGVFNIITSSDVEGVG
                     RELSTNPTVRKLTFTGSTRVGKILMQQSAGTVKKLALELGGNAPAIVFDDADLDTAVE
                     GVFASKFRNGGQTCVCANRIYVQAGIYEQFLAKLSEKVAQIKLGSPFEEEVTIGALIS
                     ERAVEKVESHINDALSLGAELVLGGERHALGGTFFQPTILKNVTQAMKVAREETFAPL
                     APIFKFETEEEVIQWANDTEFGLAAYLFSQNIGRIWRVSEQLEYGIVGINEGIISNEI
                     APFGGWKESGLGREGSKYGIEEFLEIKYLCMKV"
BASE COUNT         2672 a         2290 c         2002 g         2759 t
ORIGIN      
        1 ataaaataaa aatatgtaat aaacgcatca ttgtaaataa tgcagtaaat gtaactgaaa
       61 aaaagaaaat tgctgcaata taatcaatgg tttttgtgag atatatttcc ttttgctgta
      121 tcgcaatata gataggcact tgatgaacaa gttggaagta agcatccaaa taaacaacta
      181 tcaatatgaa gcctaaaaac cattctgttt gcataagaac attgataggg ctgttttttg
      241 tgctaaaaat ttctttaatt gaactcataa tttttcctta aatgtaaaaa aacgtacctc
      301 aaccaatttg agatacgttt ctattttaat gtaattatta aatctcactc acctccaccc
      361 accttttctc ctccgccgat tttgctatcg cttccaatac tctggagatt ttatagcctt
      421 cttcaaaatc gggatacatc gggtcatcag cggcgatgcc gttgatgagg tcacggattt
      481 ccaccgcttt ttggtcgtta aaaccgatgc cgtgaccggc actcacacaa aaagcagcgt
      541 agtcgggatg ttccgggccg actaaaatgg ttttgaatcc ttggcgggat ttatcgtcat
      601 cgtgcaaata gagtttgagt tctgccattc gttcttgggt atagctgagc gtgcctttag
      661 tacctgtaac gacataggtc agccccattt tacgcccgca ggaaatgcgg gagctttcga
      721 gcgtgcccat taccccatta gcaaatcgta tgagactact tgcctgatct tcgttttcta
      781 ctttgatttt ttctgctgga ttgtgcggac tagggcgttc ttcaatcact gtttgcatat
      841 cgccgataac acgctcaatg tctgaaccaa ccaaatagtg tgcgatactg acaatatgtg
      901 cggctaaatc tcccaacgta ccaagcccgg ctttggcttt gtagcagtgc caatcaatcg
      961 gggtattagg gttggcgaga taatcttcgt tatgcgtgcc gtagaaatgc accacctcac
     1021 caatttcgcc gttttgaata atttggcgag ccagttgggt ggttggattt ttgatatagt
     1081 tgaaaccgac caaggtttta actcccgctt gttgagcggc aatcaccatt tctttggcat
     1141 cttgggcggt gagtgcaagc ggtttttctg aatagacgtg tttgccgtgt ttaatcgctt
     1201 ctaaggcaat ttctttgtgc agaaaatttg gtgtacaaat gtccaccaca tcgacattcg
     1261 gatcggtaac caattttcgc caatcgtcag tagcacgctg aaagccaaat tctcgggctt
     1321 tttgatcggc taatgcttgg ttgatttcgg caaggtattc cagttggatt ttgcctttaa
     1381 gtggaaacag ggttggggct tgagcgtagg caatggcgtg gcagcgaccg atatagccgg
     1441 tgccaactaa gccgattttg atggttttca taatactatc ccagtagggt gcgtttgcta
     1501 acgcaccatt agaattttca gtgcattggc aaacgcaccc tataagaaag cggtctggtt
     1561 ttgcaaaatt tttacaaaat cagaccgctt gtgttagcta tctataatgc tcctcgtctg
     1621 ctcttcgcat aggtgtcgaa ggcaacggca aggatgatga ttaagcccat aatgatttgt
     1681 tggtagtaag cggaaacgtc cattagtact aaaccgttaa tcaatacacc cataataatg
     1741 gagccgatga tggtgcctgt gactcgtccg tatcccccca ttagggaagt tccgccgata
     1801 accactgagg caattacacg tagctcaaaa gaaacgccgg caacggattc ggcactgcct
     1861 aaacgggcgg agagaatgaa accggcaagt cctgccaatg ctccgatcac cacataaaca
     1921 ctgactaagg tacggttaat attcacaccg gctaaacggg ctgcttccgg attgccacct
     1981 accgagtaca catagcgtcc ccagcgggtt ttatgcaatg cgaagtaacc gataatggcg
     2041 actacggcaa aaatcactac cggcaccggt acgcctaaaa tatcgccacg tccccaccag
     2101 cggtaacctt cgtcaaatcc ggcaattggc gagccgttat ttaacacaag tgttaaacca
     2161 cgccaaattg tcataccgcc aagcgtgata ataaacggtg gcagtttgag tttagtgacg
     2221 cctaagccgt gtaaccaacc aatgaaagtg cctaaaccta aacagatact caaacctatc
     2281 agccagctca ttccgcccca agcaccggct tctagcgtta ccgtattatc gcctttgata
     2341 atataggccg cgaacattgc ggtaacagca aggattgagc cgacagagag atcgatccct
     2401 gcagttaaga tgacgaaagt cattcctacc gccataatgc cgaaaatcga cacttcggtt
     2461 aaaacgttat agatgttgcg ttgcgataaa aatgccccgt tttgtgaagt gaagaaaata
     2521 atcagtaaga tcaagaaaat cagtacgccg aaacgttcaa agaaggcgat aaaatcgatt
     2581 tttcctttgt catcgaccat acccatattt tttagaaatt gcgttgtcat acaaacctct
     2641 cttatgctgc cgctttacgc tgaacgccaa cagccatcat tgacattaat ttttcttcgg
     2701 tggcatcatc gccgtgaatt tcgccagtta cccgaccttc ggaaagagtg ataatgcggt
     2761 cggaaatcgc cataatttcc ggtaaatcgg aggaaatcac taataccgca acaccttgtt
     2821 ttgccatatc aaacagcact tggtgaacct cagattttgc cccaacatca atacctcgtg
     2881 tcggctcatc aacaattagt actttcgggt tgagtgccat acaacgggct aaaatcactt
     2941 tttgctgatt accacccgat aatttgcggg cttcaagttc gctacttgcc attttgatgc
     3001 gtaacgcttg gcggtaggtt tcgattaaat catcttcttt ttgattatcg acaaacaagt
     3061 tacccttcaa taatttgctt aaaatcggca gggtcatatt ggttttaatc ggtaaaccga
     3121 gaactaagcc ctcttttttg cgatcttccg gcacaagggc aacccctttt tccaaagcat
     3181 ctaacggcga ggtggcagca taaggcttac cgtctaggct gatttcatca gccgtataat
     3241 tttctacccc aaacaagcaa cgggcaattt cagttcgccc tgcgccaact aagccggcga
     3301 tccctaatac ttcaccctcg tgaatgttaa aagagacacc gtggagagca atgccgtgtg
     3361 gatcgaatgc cggtttttcc cgatgcagat ttttcacagc taaacgtatt ggtcggtggg
     3421 tatggtgagt ctcttctggt ggacggcgag taaaggcaac ttcacgtcct accatcattt
     3481 taataatttg atcgacattc acatttttga cttcaccctc accagaatag cgcccgtctt
     3541 ggaaaacggt gaaacggtcg catagggcaa acacttcgtt taagcggtgg gtcacataaa
     3601 tcacgctaat gccacggctt tttaaatcac gcacgatttg gtgtagtgtt tccacttctt
     3661 tatcacttaa ggcagcggac ggttcgtcca taataatcaa cttagcgttt aaggtcattg
     3721 cacgggcaat ctcgaccatc tgctgttgtg ccacacttaa acgggaaact tgggtgtcag
     3781 gtttgatatt taagccgaga ttatccagca ctaattgtgc ttcctcattc accgcttttt
     3841 cattgacgaa taaacctttc accggctctc tgcccaaaaa gaaattttca gcaaccgtca
     3901 tattcggtaa caagttaaat tcttgataaa tcgttacgat gcctttgagt tggcgatcaa
     3961 acggggaatc gctcaaactt aaggtttcac cgttaaagat aatttcgcca cttgtttgtg
     4021 gctgagcgcc tgaaagtgct tttaataagg tcgattttcc tgcaccgttt tcacccaaca
     4081 atgcgtgaac ttcgcctttt cccacagtca gtttcacgcc attgagtgcc ataacaccag
     4141 agaaactttt tgtcagatct ttcacttcaa gtaagggagt agacatacaa cctccgtaaa
     4201 aaaacgccct ccggcctcag ccggaaggca gataaattat tcgctgattt ctgcaaaacg
     4261 ttctgcttct tgcagattat ctttcaggat aatttttggc ggatagtccg caccgctcac
     4321 ttctttgctc tcacggatat tgccaaccac ttgctctaat gcggtgctta ccgcaaaact
     4381 tgggcgttgg tcagcggtga gatacaacca gccatctttc acacgtgcaa gagcctcagg
     4441 ggttgagtca aagccggtta ctaaaatatc gccggctttc aaaccttggc tacgcaatgc
     4501 ttcaatcgca ccaagtgcca tatcgtcatt cgctgaaata atcacttctg gtttctcttt
     4561 taaggttggt aacacacttt caataatgcg taatccctca gaacggagcc agttaccggt
     4621 ttgatcaacc acgattttat atttgtcacc accggcagct aattcatcac gaataccttt
     4681 ggtacgttca atgttagagg ttgaacccgg ctggccggtt aaaagaataa tttttgcccc
     4741 attcgggtat ttggctttca ctgcttttgc gacttcttga ccgcctgtgt agttatttgc
     4801 cccaaagtgt ggaaccggct tactgctttc aacttttcgg tctaatgtcg cagcaggaat
     4861 tttttctttg ataatttctt ccactgcacc tgaaatcgca ttcacatcat tcggggaaat
     4921 gataatgcct ttagcaccac gggtaattgc attttctaag tcagaagcct gcttagtaga
     4981 agaactttgc ccgtctaata cttgcaattt gatctccaac tttttactgg tttctaccgc
     5041 tactttttgt agttgaactt caaacgggct ggataggttc ggtaagctaa ataccacaag
     5101 ctcatctttc gcaaagctgt tcattgctaa gcctaacgtt aaagcactca atacaaattt
     5161 aatacgcttt ttcattacaa aatcctcttt ttaagatggg aattgacggc gaatattgcc
     5221 aatgttactt caaactgcga aggaaactta agtcttattt caataataaa cctcaatgaa
     5281 ataaattttt caatttagaa tatatcattc aaaattttcc aaaccaaatg ctttgtttca
     5341 aatttgttac gtagatcaca tttttaggaa gagataaatg caagcggtca aaacaggtcg
     5401 cttttttgca aaaatttgaa taaaaatgac cgcttgtgag atgaaaacca ataaaaaagc
     5461 ggtgatttct caccgctaaa cactcaaaaa agaaatgtac tcgttattat aaggttggga
     5521 aattaaatgc cgcttggcta cgcaccgagc tgtccggcca acgggttgtg atggttttca
     5581 tacgggtgta gaaacgcacg ccgtctgtgc cgtacacatt gagaggcccg aaaatagagg
     5641 ctttccaacc gccaaagcag tggaacgcca ttggcacagg aattggaata ttcacgccca
     5701 ccataccggc ttgtacatct tgggtgaatt gacgtgctga atcgccatct gcggtgaaaa
     5761 ttgcactacc attgccgtat tggtgactgt taatgagtgc gatcccttcg gcataatctt
     5821 tcacacgcac aacagagaga accggtccga agatttcatc ttgccagatg gtcatttcag
     5881 gtgtcacgtt atcaaacagc gtacctccga caaagtaacc gttttcaaac ccttcgactt
     5941 tatagcctcg accatcgaca cagagtttcg ccccctgtgc caccccttga tcgatatagc
     6001 tctcaatttt ggctttatgc tctttggaaa tcaccggccc catatcattt tcaagcacgc
     6061 cttgtggtag taagcctgaa ccgattttca atgctttgac cttaggaatt agctcttcaa
     6121 tgattttatc ggctaattca tcaccaacaa ctaccgctac cgatagtgcc atacaacgtt
     6181 caccggctgc accaaatgct gcgcccagta aggcattaat tgtgccttgt acatcagcat
     6241 ccggcataat caaagcgtgg tttttcgcac cgcccaaggc ttgaacccgt ttgccgtttg
     6301 ctgagccttt ctcataaata tattgtgcaa ttgcagttga gccgacaaaa ctcaccgcct
     6361 gaatgcgaga gtcggttaaa agaatatcta ccgcttcttt atcaccttgc accacattga
     6421 atacaccgtc cggcaagccg gcttctttta ataattctgc caagcgaata gacacacttg
     6481 ggtctttttc agacggtttt aaaacgaaag tgttaccgca agcaagtgcc actgggaaca
     6541 tccacatcgg taccattgcc gggaaattga acggggtaat gcctgccact acgcccaacg
     6601 gttgctggat tgagtgaata tcaatgccac gccctgcatt ggcggagaac tcaccttttt
     6661 gtaagtgtgg aataccagtg gcaaattcca ccacttctaa accacgggtt aattcgccaa
     6721 tcgcatcgga atagattttg ccgtgttctg agctgattaa acgagccaac tcatcaaagt
     6781 ttgcttccaa taactctttg aatttaaata aaattctggc acggcgtagt ggtgattgtt
     6841 gtgaccagct tttgaaagcc tgatcggccg ctgcaattgc cgcatcgact tcgtttggca
     6901 ggcttaattt gacttttttg gtctcttcac cggttgcggg attaaatacc gaggtaacac
     6961 gctggctttg gctttcaacg actgcaccgt taataaaatt ttggacaaat tggctcattg
     7021 gaggctcctt atcaactgat tgagaggaaa aacggataaa atacagccga taaactaccg
     7081 ctaaaaatgg aaaatatcaa tacataatac taaaaaatga aaattttatt tcatttttgt
     7141 gatctaagta gcaagtttac agattgccgg tattaaaatg agacaaaatt tcgtatagtt
     7201 tgaaagcgta ttccatttcg ttaagcgagg tttcctatgt ctagtccatt attgtctaaa
     7261 cccaatttca accaacatga agggttaatt cagcacatta cccctgaaaa tgcacagtgg
     7321 gaatatgttg gatttgatgt ttatcagctc agtaagcaac aaattacgct ggaaagtggt
     7381 gaaaatgaac tttgtctagt ctttgtgacc ggttttggct ctgtctatgt tgatgattat
     7441 gcttttgaaa atatcggcaa ccgtaccacg ccatttgaaa aaattccgcc ttatgcggtc
     7501 tatatcccgc cacacaaaag cgtgttggtt aaaaccgaac tcaatttaga attggcagtg
     7561 tgcaaagcac caagtcaggg taattatccg gtgcgtttga ttagcccgaa ccatatcggt
     7621 gtggaaaaac ggggctttgg caacaatcaa cgcttggtgc ataatattct gccggaaacc
     7681 gaagcagccg acagtttgct ggtggtggaa gtctttaccg atgaaggcaa taccagttcc
     7741 tacccaagcc acaagcacga tgacaaacac tctaccgaag aaacctattt ggaagagacc
     7801 tactaccacc gcttcgatcc accacaaggt tttgcaatgc aacgggttta taccgatgat
     7861 cgcacacttg atgaatgtat ggcggtgtat gataaaaatg tggttaaagt gcctcgtggc
     7921 taccaccctg ttgctaccat tgccggctat aacaactact acctaaatgt tatggcaggg
     7981 ccggtacgca aatggaaatt tacttgggaa caagatcatc tttgggtgaa cagtgaaacc
     8041 tataaagcca aatttgagta atgcaagcgg tctttttcca ccaatttttt acaaaataag
     8101 ggattaaaaa tgggacgctt ttggcgtccc attcggttat actttcatac aaagatattt
     8161 tatttctaaa aactcttcaa taccgtactt cgaaccttcc cgcccaagtc cagactcttt
     8221 ccagccgccg aacggagcga tttcgttact gatgatcccc tcattaatcc cgacaatgcc
     8281 atattccaac tgctccgata ctcgccaaat tcgcccgata ttttggctga ataagtaagc
     8341 cgccaaacca aactcagtat cattcgccca ctggataacc tcttcttcgg tttcaaattt
     8401 gaaaatcggg gcaaggggag caaaggtttc ctcacgagcc actttcatcg cttgagtgac
     8461 atttttcaga atggttggct ggaagaatgt gccacccagt gcgtggcgtt caccgcctaa
     8521 caccagttct gccccaagtg aaagggcatc gttaatgtgg ctttcgactt tttccaccgc
     8581 tcgttcacta attagtgcac ctatcgtgac ctcttcctca aatggcgagc caagcttgat
     8641 ttgagccact ttctcactca attttgcaag aaattgctca taaatgcccg cttgcacata
     8701 aattcggtta gcacagacgc aggtttgccc accgttacgg aatttagagg caaacacgcc
     8761 ctcaaccgct gtatctagat ccgcatcatc aaacacaatc gccggtgcat taccgcctag
     8821 ctccaatgcc aattttttta ccgtccctgc cgattgttgc attaaaattt tgccgacacg
     8881 ggtggagccg gtaaaggtga gtttgcgtac ggtcggatta gtgctcaact cccgccctac
     8941 accttcgaca tcgctactgg taataatgtt aaatacccct ttgggaatac ctgcttgatc
     9001 cgctaattgg gctaatgcta aggcagaaag cggggtttct gccgccggtt taatcacaat
     9061 cgcacaacct gccgctaacg ccggtgccgc tttacgggta atcattgcat ttgggaaatt
     9121 ccacggtgta attgctgcca ccacgccaat gggttgttta gtcacaaaca agcggcgacc
     9181 ggctttatct tgtggcaaga catcgccata aatccgcttg ctttcttcgg caaaccactc
     9241 gataaaactg gctccatagg caatctcgcc tcgactttct gccaatggtt taccctgttc
     9301 tagacttaaa atctgggcga gttcttcttg gttttgcata atcagctcaa accatttacg
     9361 caaaatcacg gaacgctctt tgggcaataa tgcctgccat tttttctggg caactgcggc
     9421 tgcttcaatc gcttgagcgg tttcttccgt gcctaaatca gccacctcac cgattttctc
     9481 accattagtc ggattctgta ccgtaaaggt tttcccattt tggcttgaaa caaatttacc
     9541 gttaatatag gcttggaaac ggagtagcaa aaatttgtga tttggcataa tcactccttg
     9601 aatggttaaa aagagaacaa tattgactat aatccaattt cagtaaagag aaaagagctg
     9661 aaaaataact ttttactcaa taataaagca ccttagacaa gcgttcttct aaggtgcagc
     9721 cat
//
LOCUS       DANLRK010000097         9717 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.097, whole genome shotgun sequence.
ACCESSION   DANLRK010000097 DANLRK010000000
VERSION     DANLRK010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9717
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.097"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            46..726
                     /locus_tag="PXA33_001688"
     CDS             46..726
                     /locus_tag="PXA33_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007523984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00153 family protein"
                     /protein_id="HDL5121002.1"
                     /translation="MALNNILGLFAQSPLKPLQKHSNKVTECSELLESFFEATFEHDW
                     EKAAEVRGKIVDLERRADALKREIRLKLPRGLFMPVERGDLLELVTQLDKLANYSRDI
                     SGRIIGRKLIIPTEMQPLFKKFLSRSIDASRQVRKVLDEMDQLLETGFRGRELDFVNK
                     MILELDQIEDDTDQYQITLRHTLLELEKNLNPIDVMFLYKCIERISVLADQAQRIGSR
                     IELMLAKS"
     gene            738..2006
                     /locus_tag="PXA33_001689"
     CDS             738..2006
                     /locus_tag="PXA33_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HDL5121003.1"
                     /translation="MELLNQYGSILVIITAIVGFMMAFGIGANDVSNAMGTSVGSGTI
                     TIKQAILIAMVFEFAGAYLAGGEVADTIKSGIIDPAQFANSPDILVLGMMASLCAAGM
                     WLIIATKMGWPVSATHTIIGAVIGFALITIGSDAIQWDALGGIVGSWFVTPILAGIVA
                     YSIFINSQKLIFNRSNPFKQAKKYGPMYMGITIFILVIVTVSKGLKHVGLHLDTAETL
                     GISFALALLAFIIGYFYFRSKSFAMKARAEEKGFAGVEKIFSILMLITACAMAFAHGS
                     NDVANAIGPLAAVESIVRSGGMIEGKTTLAPWILPLGALGMMIGLAIMGKNVMATVGT
                     GITDLTPSRGFAAQFACAVTVVIASGTGLPISTTQTLVGAVLGVGFARGIAALNLGVI
                     RNIVISWVITLPVGAVLSIIIYYILSAIFY"
     gene            2061..2672
                     /locus_tag="PXA33_001690"
     CDS             2061..2672
                     /locus_tag="PXA33_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HDL5121004.1"
                     /translation="MKKLSSALLSCFLLGLSSPALTADTYITENLSTFMRKGAGDQFR
                     ISGAIQAGEKVTVLDRKERYSLIRDSRNREGWVLNSDLSDTASPKELIPQLKQQVQDL
                     TNRLSKIDTEWQQRTAEMQRRTQDSVQKSSDLVEENAQLKRELEILKNKNRDLETMHD
                     SEKREIVIQWFIYGGAVLGAGLLLGLLVPFLIPRRRRNNSGWA"
     gene            complement(2726..4024)
                     /gene="aroA"
                     /locus_tag="PXA33_001691"
     CDS             complement(2726..4024)
                     /gene="aroA"
                     /locus_tag="PXA33_001691"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HDL5121005.1"
                     /translation="MEKLTLTPISRVEGEINLPGSKSLSNRALLLAALATGTTQVTNL
                     LDSDDIRHMLNALKALGVKYELSDDKTVCVVEGIGGAFKVQNGLSLFLGNAGTAMRPL
                     AAALCLKGEEKSQIILTGEPRMKERPIKHLVDALRQVGAEVQYLENEGYPPLAISNSG
                     LQGGKVQIDGSISSQFLTALLMSAPLAEGDMEIEIIGDLVSKPYIDITLSMMNDFGIT
                     VENRDYKTFLVKGKQGYVAPQGNYLVEGDASSASYFLASGAIKGKVKVTGIGKKSIQG
                     DRLFADVLEKMGAKITWGEDFIQAEQSPLKGVDMDMNHIPDAAMTIATTALFAEGETV
                     IRNIYNWRVKETDRLTAMATELRKVGAEVEEGEDFIRIQPLALENFQHAEIETYNDHR
                     MAMCFSLIALSNTEVTILDPNCTAKTFPTYFRELEKLSVR"
     gene            complement(4070..5164)
                     /locus_tag="PXA33_001692"
     CDS             complement(4070..5164)
                     /locus_tag="PXA33_001692"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HDL5121006.1"
                     /translation="MQFINIANEGVKSLSPYQAGKPIEELERELGIQNIIKLASNENP
                     FGFPESAKKAILNQLDDLTRYPDSNGFELKTTIAQKFGVQANQITLGNGSNDLLELFA
                     HTFAGEQDEIIYSQYAFIVYPLVTKAINAVAREILAKNWGHNLDAFLAAITEKTKLIF
                     IANPNNPTGNFLTQAEIEGFLAKVPANVIVVLDEAYTEFTAPQERVDSFGLLAKYPNL
                     IVSRSLSKAYGLAGLRIGYAVSNPEIADLLNRVRQPFNCNSLALASAIAVMNDDEFVK
                     KVAENNRLEMARYEAFCQANGLEYIPSKGNFITIDFKRPAAPIYEALLREGVIVRSIA
                     GYGMPNHLRISIGLPQENERFFTALLKVLA"
     gene            5436..6755
                     /locus_tag="PXA33_001693"
     CDS             5436..6755
                     /locus_tag="PXA33_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HDL5121007.1"
                     /translation="MNHLLYSVEDKPPFGLSLLLASQHLLAALGGIIAVPLVLGNVLK
                     LSTADTIILVNAALLISGIVTIIQCRGIGPIGIRLPSVMGTSFTFVAAALAIGFSESG
                     IAGIMGASLVGSLVMIIGSFFMPYIRKLFPPVVTGTVIMMIGLSLIPVAVDWFAGGQR
                     TDPHYATPENLIMASFVLIIVVALVQWGKGIFSAAAIVIGMMIGYIVSLALGWVSFNG
                     VKEAQLFALPQPLHFGLEFPISGIIGMSIAYLVTIVESSGNFLALGNATKTEITGKHL
                     RGGVLCDGLGSAFAAIMSTTPFSSFSQNIGVISLTGVASRYVVAFTGALLVLAGLFPV
                     FGALIVSIPLPVLGGAGLMMFAMIIAAGIQMLDNIQRSKRNGLIIAISIGCGLAVTTR
                     PELLDKLPHFFKEVFGSGITVGSLLALILNLVLPEDKITKKENKSDE"
     gene            complement(6925..8868)
                     /gene="ftsH"
                     /locus_tag="PXA33_001694"
     CDS             complement(6925..8868)
                     /gene="ftsH"
                     /locus_tag="PXA33_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HDL5121008.1"
                     /translation="MVKNILLWVVIAIVMMTAYEGFNSNFSGSSNTVPYSTFLSDIKE
                     NRLKGVDFKQNDDTILVTKNDGATYQTVMPMYDEYLMADLAKTNAVITGQPAERRGLL
                     SQILISWFPMLLLIGFYVFYMRQMQGGGGRGAMGFGKNKAKMLTADEVKTRFSDVAGC
                     DEAKEEVGEVVEFLRDPTKFQKLGGRIPKGILMVGPPGTGKTLLAKAIAGEARVPFFT
                     MAGSDFVEMFVGVGASRVRDLFDQAKKNAPCIIFIDEIDAVGRKRGGAGFSGGHDERE
                     QTLNQMLVEMDGFEGSEGVIIIAATNRADVLDDALTRPGRFDRQVTVDLPNVKGREQI
                     LKVHLKKVPLAEGVDPMQIARGTPGYSGAQLANLVNEAALFAARKNKRVVMMEDFEEA
                     RDKINMGPERRSNTMTEKEIINTAYHEAGHVIVGYLMPEHDPLNKVTIVPRGQALGFA
                     QFLPEGDRVSETFTKLESQLSTLFAGRIAEGLIFGEDKITTGASSDIHRATQIARAMV
                     TQWGFSKELGPIFYQNEDGMGAIKGVSEESQKLIDVEMRKIIDRNYQRAKQTLEENMD
                     ILHAMKDALLKYETLDSKQIDDLMARRPVGEPSGWTDTPTKDDNATPPSGEQAADVSS
                     TLEDNPNKDNNIGDSLADTKAEH"
     gene            complement(8989..9615)
                     /gene="rlmE"
                     /locus_tag="PXA33_001695"
     CDS             complement(8989..9615)
                     /gene="rlmE"
                     /locus_tag="PXA33_001695"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011200140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HDL5121009.1"
                     /translation="MGRKRSASSSRWLAEHFKDQFVQKAHKQKLRSRAYFKLDEIQQT
                     DRLFKPGMTVVDLGAAPGGWSQYVVTQIGSKGRVIACDILDMNPIVGVDFLQGDFREE
                     SVLNALLERVGEDKVNVVMSDMAPNFSGMPSVDIPRAMYLVELALDMCRQVLATKGSF
                     VVKVFQGEGFDEYLREIRSLFSVVKVRKPEASRDRSREVYIVATGYKG"
BASE COUNT         2719 a         2080 c         1892 g         3026 t
ORIGIN      
        1 tacagtaaga agtcattgtc aatattatca ctatggagat attttatggc attgaataac
       61 attttaggtt tattcgccca gtccccactt aaaccgttac aaaaacactc aaataaagtg
      121 actgaatgta gcgagttatt agaatctttt tttgaagcga cttttgaaca tgattgggaa
      181 aaagcagctg aagtgcgcgg taaaattgtt gatttagaac gccgcgcaga tgcccttaaa
      241 cgtgaaatcc gcttaaaact gcctcgtggt ttatttatgc cagttgaacg cggtgattta
      301 ttagaactag tcacacaatt agataagtta gcaaactatt caagagatat ttccggtcgc
      361 attattggtc gtaaattaat cattcctacc gaaatgcaac cacttttcaa aaaattcctt
      421 tctcgtagta ttgatgctag ccgtcaggta cgcaaagttt tggatgaaat ggatcagtta
      481 ttagaaactg gttttagagg gcgagagctt gatttcgtca ataaaatgat tcttgaactt
      541 gaccaaattg aagatgatac cgaccaatat caaattacat tacgtcatac acttcttgag
      601 ttagaaaaaa acctgaaccc tattgatgtg atgtttttat acaaatgtat tgaaagaatc
      661 agcgttctag ctgaccaagc ccagcgaatt ggttctcgta ttgaacttat gctggcgaag
      721 tcataggaga aaattaaatg gaattactca atcaatatgg ctcaatactg gtgattatta
      781 ctgctattgt agggtttatg atggctttcg gtattggtgc taatgatgtt tcaaatgcca
      841 tgggaacatc cgtcggctct ggaacaatta cgattaaaca agctatttta attgctatgg
      901 tatttgaatt tgctggtgct tatctggcag gcggtgaagt cgcagataca attaaaagtg
      961 gcattattga tccggcacaa ttcgccaata gccctgatat cctggtactt ggaatgatgg
     1021 catctctctg tgctgccgga atgtggctaa ttattgcaac taaaatgggt tggccggttt
     1081 cagcaactca cacaatcatt ggtgccgtta ttggttttgc attaatcact attggttcag
     1141 atgcaattca atgggatgca ttaggtggaa ttgttgggag ttggtttgtg acccctattc
     1201 ttgcggggat cgtagcatat tcgatcttta ttaactcaca aaaactgatc tttaaccgct
     1261 caaacccatt caaacaagcg aaaaagtatg gcccgatgta catgggaatt actattttta
     1321 ttttagttat tgtaaccgtg tcaaaaggct taaagcacgt aggattacat ctagatacag
     1381 ctgaaacatt gggaatttca ttcgcgcttg ccttacttgc ttttatcatt ggatattttt
     1441 atttccgcag taaaagcttt gcaatgaaag ctcgtgcaga agagaaaggc ttcgctggcg
     1501 ttgagaaaat ttttagtatt ttaatgctta ttacggcctg tgctatggct tttgctcatg
     1561 gctctaatga tgttgctaat gctattgggc ctctagctgc tgttgaatct attgtaagaa
     1621 gtggtggaat gattgaaggt aaaacgactt tagccccttg gattctccca ctaggcgcct
     1681 taggtatgat gattggttta gctattatgg gcaagaatgt aatggctaca gtaggtactg
     1741 gtattaccga ccttactcca agccgtggct ttgctgctca atttgcctgc gccgtcacgg
     1801 ttgtcattgc atcaggtact ggtttaccaa tatcaacaac ccaaacgctt gtaggagcag
     1861 tattaggtgt gggattcgcc cgtggtattg ctgcacttaa cttaggcgtt attcgtaata
     1921 ttgttatatc atgggtaatt accttgcctg taggtgctgt gctttcaatt attatctatt
     1981 atattttaag cgctattttc tattaatttt tcaaaaagaa tacaggcaac tgtattcttc
     2041 ctagctttag aggaatatat atgaagaaat tatcatcagc tcttctgtct tgttttttac
     2101 tcggcctatc tagccctgct ttaacggcag acacttacat tactgaaaat ctgagtacat
     2161 ttatgcgcaa aggtgctggt gatcaattcc gcatttcagg cgctattcaa gcaggtgaaa
     2221 aagtcacagt attagatcgt aaagagcgct acagtctgat tcgagatagt cgtaaccgtg
     2281 aaggttgggt acttaattca gacttaagcg atactgctag tccaaaagag ctgattcctc
     2341 aattaaaaca acaggttcaa gatttaacca atcgcctgag caaaatcgat accgaatggc
     2401 aacaacgtac cgcagaaatg caacgtagaa cgcaagattc agtgcagaaa agcagtgatt
     2461 tggttgagga aaatgcacaa ctaaaacgtg aactggaaat cctgaaaaac aaaaatcgtg
     2521 atttagaaac aatgcacgat tccgagaaac gtgaaatcgt gattcagtgg ttcatctatg
     2581 gtggtgctgt gttaggcgca ggtttactac ttgggttact cgtgcctttc ctgatccctc
     2641 gccgcagacg taacaacagc ggttgggctt agtctgccac aataaaacgt aaaaaggatt
     2701 cagttttctg aatccttttt tacttttatc tgaccgataa tttttccaac tccctaaagt
     2761 aagtcgggaa cgttttagcg gtacaatttg gatctaagat cgtcacttct gtattcgata
     2821 acgcaattaa tgaaaaacac attgccatac ggtgatcgtt ataggtttca atttcagcgt
     2881 gctggaagtt ttctaacgca agcggttgaa tccgaataaa atcttcccct tcttctacct
     2941 ctgccccgac tttacgcaat tcggttgcca ttgctgtcaa gcggtcggtt tcttttaccc
     3001 gccagttata aatattgcgg ataactgttt ctccttcggc aaataaagcg gttgttgcaa
     3061 tcgtcattgc cgcatcagga atatgattca tatccatatc tacgcctttt agcggggatt
     3121 gctcggcttg aataaaatcc tctccccaag tgatttttgc ccccattttt tccaacacat
     3181 cggcaaacaa gcggtcgcct tggatcgatt ttttaccaat gcccgttact tttaccttgc
     3241 ctttaatcgc accggaggct aagaaataag aggcagaaga ggcatctccc tccaccaaat
     3301 aattaccttg tggagcaaca tagccttgtt tacctttaac taaaaaggtt ttgtaatctc
     3361 gattttcaac cgtaatacca aaatcgttca tcatcgaaag ggtaatatca atataaggtt
     3421 ttgataccag atcaccgata atctcaattt ccatatcgcc ttccgctaat ggggcagaca
     3481 tcagcaatgc ggttagaaat tggctggaaa tcgagccgtc aatttgcact tttccgccct
     3541 gcaaaccgct attgctaatt gccaacggtg gatagccttc attttctaaa tactgtacct
     3601 ctgcccctac ttggcgtaaa gcatcgacta agtgtttaat cgggcgttct ttcattcttg
     3661 gttcaccggt aagaatgatt tgggattttt cctcaccttt taaacacaat gctgctgcaa
     3721 gtggtcgcat tgccgtgcct gcattgccga gaaacagtga taagccgttt tgaaccttaa
     3781 aagctccacc aatcccttca actacacaga cggttttatc gtccgatagc tcatatttca
     3841 cgcctaacgc ttttaaggca ttgagcatat gtcgaatatc atcactatct aataaattgg
     3901 tcacttgagt cgtaccggtg gctaaggcgg ctaataataa ggctcggtta gacaggcttt
     3961 tagaaccagg taaattgatc tcgccttcta ctcgggaaat cggggttaaa gttagttttt
     4021 ccatttttct ctttaaattt gtaaaatttt cataaaaggt aaccgcttgt taagctaaca
     4081 ctttcaataa ggcagtaaaa aatctctcgt tttcttgcgg taaaccgata ctaatgcgta
     4141 aatgattcgg cattccataa cctgctatcg agcgaacaat cacgccttca cgcagtaatg
     4201 cctcataaat cggagcggca ggacgtttga aatcaatagt aataaaattg cccttagaag
     4261 ggatatattc caagccgttt gcttggcaga aagcctcata gcgagccatt tccaagcggt
     4321 tattttctgc cacttttttg acaaattcat catcattcat taccgcaatc gctgaagcta
     4381 aggcaagact attgcagtta aacggctggc gaacacgatt taataaatcc gcaatttcag
     4441 ggtttgaaac cgcataacca atccgaagcc cggctaagcc gtaagcctta gagagtgaac
     4501 gtgacacaat taaattcggg tattttgcca ataagccaaa cgaatccacc cgttcttgag
     4561 gcgcagtaaa ctcggtataa gcttcgtcca gcaccacgat tacatttgcc ggcactttag
     4621 ctaaaaagcc ctcaatttcc gcttgggtta aaaaattacc ggtcggatta ttcgggttgg
     4681 caataaaaat taatttagtt ttttcggtaa ttgcagccaa aaaggcatct aaattgtgtc
     4741 cccaattttt agccagaatt tcacgggcaa cggcattaat cgctttcgtc accaacggat
     4801 acacaataaa cgcatactga gaatagataa tttcatcttg ctcaccggcg aaggtgtggg
     4861 caaacagctc taataaatcg tttgagccat tgcctaaggt gatttgattg gcttgtacgc
     4921 caaatttttg ggcaatagtg gtttttaatt caaagccgtt tgaatccgga tagcgggtta
     4981 aatcgtcaag ctgattcaga atcgcctttt ttgcactttc aggaaaacca aatgggtttt
     5041 cgttcgaggc taatttaatg atattttgaa tccctaattc acgctctagc tcctcaatcg
     5101 gctttcctgc ttggtaaggc gaaagggatt tcacgccctc atttgcgatg ttgataaatt
     5161 gcataataac ctctgattat tcttataaaa tataagttcg gattgtagta aagaatgatt
     5221 gcctttacca gtaaattttt tgttacaact ggaaagaaat tggtacaaac attcggatta
     5281 gctttgcaaa aagcgtaaaa aatcttaccg cttgtcattt atcagttagg gaaaataaaa
     5341 aatcactctc ttatataaaa acaattacgc aaacgattgc ttattaaaaa aacttcagta
     5401 taatgccgac attttttaac tctcgaggaa tcactatgaa ccatttactt tattctgtcg
     5461 aagacaaacc gcctttcggg ctgagcttat tgcttgcctc acaacattta ttagccgctt
     5521 taggtggcat tatcgctgta cctttagtgc tagggaatgt actcaaactc tctactgcgg
     5581 atactataat cttagttaat gctgcacttt taatttcagg catcgtcact attattcaat
     5641 gtcgaggtat tggcccaatc gggattagac taccgagtgt aatgggaact agctttacgt
     5701 ttgttgcagc agcattagcg attggtttca gtgaaagtgg cattgccggc attatgggag
     5761 cctctttagt tggctctcta gtgatgatta tcggcagttt ttttatgcct tatatccgta
     5821 agctttttcc acccgtggta acaggtactg tcataatgat gattggccta agtttaattc
     5881 ctgttgcagt agactggttt gctggtggtc agcgtactga tccacactat gcaaccccag
     5941 aaaatcttat aatggctagt tttgtattaa ttattgtcgt tgctttagta caatggggga
     6001 aaggtatttt ttcagctgct gctattgtga ttggtatgat gataggttat atcgtttcgc
     6061 tagctctggg ttgggtaagt tttaatggcg tgaaagaagc acaacttttt gcgttaccac
     6121 aacctctaca ttttggctta gaatttccaa tctccggtat catcggaatg tcaattgctt
     6181 atttagttac cattgtagaa tctagcggaa atttcttagc actaggcaat gcaactaaaa
     6241 ctgaaattac cggaaaacac ttacgtggag gtgtattatg tgatggttta ggctcagcat
     6301 ttgcggcaat tatgtccaca actccctttt cttctttctc acaaaatatt ggggtcattt
     6361 cattaaccgg tgtagccagt cgctatgttg tagcatttac aggcgcatta cttgtactag
     6421 ccggtttatt tcctgtattt ggagcattaa tcgtgtctat cccgttacct gtgcttggcg
     6481 gtgcaggctt aatgatgttt gccatgatta tcgcagccgg tatccaaatg ttagataata
     6541 ttcaacgtag caaacgaaac ggtttaatta ttgctatctc tatcggttgt gggctggccg
     6601 taacaactcg acctgagcta ttggataagc ttccacactt ctttaaagaa gtttttggct
     6661 caggtattac ggtcggctcc ttattagcac ttattttgaa tttagtttta cctgaagata
     6721 aaataaccaa gaaagaaaat aagagtgatg aataaatcac cctaaaaaaa caaggacagc
     6781 tcacaataca actttgatgc tgtcctttat tcccatagag agcatattta aatttaggtt
     6841 aataaaaaaa tctgcactct atattggtgt ttacctttag agtacagatt tcttcttatt
     6901 ctaactttct agcaaacaat tagattaatg ttccgctttg gtatcggcta acgaatcccc
     6961 aatgttatta tctttatttg gattatcctc taaagttgaa cttacatccg ctgcctgttc
     7021 accacttggt ggagtggcgt tatcgtcttt agttggggta tctgtccaac ctgatggttc
     7081 acccaccgga cggcgtgcca ttaaatcatc aatttgcttg ctgtctaagg tttcgtattt
     7141 caataaggca tctttcattg cgtgtaagat gtccatattt tcttccaaag tttgcttcgc
     7201 tcgttgatag ttgcggtcaa tgatcttacg catttccaca tcaatcaatt tttgcgactc
     7261 ttccgatacg cccttaattg cacccattcc atcttcattt tgatagaaaa tcgggcctaa
     7321 ttcttttgag aagccccatt gagtaaccat tgctcgtgca atttgggttg ctcgatgaat
     7381 atcagaggaa gcccctgtgg taattttatc ttcaccaaaa atcaagccct cagcaattcg
     7441 ccctgcaaat agggtggaaa gttggctttc caatttggta aatgtttcgc ttacacggtc
     7501 accttccggt aagaattgag caaaacctaa cgcctgacca cgaggcacaa ttgtcacttt
     7561 attcagcgga tcgtgttccg gcattaaata gccaacaata acgtgtccag cttcgtggta
     7621 agcagtattg atgatctctt tttccgtcat tgtatttgaa cggcgttccg gccccatatt
     7681 gattttgtca cgggcttctt cgaaatcttc catcattacc acacgtttat tcttacgggc
     7741 agcaaataag gcagcttcgt tcaccaagtt tgctaattga gcaccagagt aacccggcgt
     7801 accacgagca atttgcatcg ggtcaactcc ctctgcaagc ggtacttttt tcaagtgaac
     7861 tttcaaaatt tgctcacgcc ctttcacgtt cggcaaatcg accgtgactt ggcggtcgaa
     7921 acgccccgga cgagttaagg catcatctaa cacatcggca cggttggttg cagcgatgat
     7981 aatcacacct tcagagcctt caaagccgtc catctcaact aacatttggt tgagggtttg
     8041 ttcacgctca tcgtgtccac cgctgaaacc tgcaccacca cgtttacgcc ctaccgcatc
     8101 aatttcatca ataaaaataa tacaaggggc atttttcttg gcttggtcga ataaatcacg
     8161 cacccgtgaa gcacccacac ctacaaacat ttccacgaag tcggaacctg ccattgtaaa
     8221 gaatggcact cgtgcttcac ctgcaatcgc tttcgccaat aaggttttac ccgtacccgg
     8281 agggcctacc atcaagatcc cttttggaat gcgcccacct aatttttgaa atttagttgg
     8341 gtcacgcaaa aactcaacaa cttcgcctac ttcttctttg gcttcatcac agccggctac
     8401 atcagaaaat ctggtcttaa cttcgtctgc ggtcaacatt ttggctttat ttttcccaaa
     8461 gcccattgca ccacgtcctc cgccaccttg catttgtcgc atataaaaca catagaaacc
     8521 gattaacagc aacattggga accaagaaat caaaatttgc gagagcaagc cacgacgctc
     8581 agcaggctga ccggtaatca ctgcattggt ttttgctaaa tctgccatta agtactcatc
     8641 atacatcggc attacggttt ggtaggttgc accatcattt ttcgtcacca aaatggtgtc
     8701 atcattctgt ttgaaatcga ccccttttaa acgattttct ttaatatcgc ttaaaaaagt
     8761 cgaatatggc actgtatttg agctaccact gaaattagaa ttaaaacctt cataggctgt
     8821 catcatcaca atggcaatca ccacccaaag taaaatattt ttaaccatat cgttcaaggt
     8881 taatccccta tctgctgaat aaaaaactgt tcaagattac tataaaagcg gtcgaatttt
     8941 ccaatttttt tgcaaaatgt taataaaaaa tgaccgcttg cttgttttct agcctttata
     9001 acctgtcgct acaatataaa cttctcgaga ccgatctcga gacgcctccg gttttctgac
     9061 ttttaccaca ctaaagagcg agcggatttc tcgaaggtat tcatcaaatc cttcgccttg
     9121 gaatacctta accacaaaac tgcctttggt tgctaatact tggcgacaca tatccaatgc
     9181 caattccact aaatacattg cacgaggaat atcaaccgac ggcataccgc tgaaattggg
     9241 agccatatcc gacatcacca cattcacttt gtcttcgcct actcgctcaa gtaacgcatt
     9301 cagcaccgat tcttcacgga agtcgccttg taagaaatcc acgcctacaa tcgggttcat
     9361 atctaaaata tcacaagcga ttactctgcc cttactgccg atttgcgtca ccacatattg
     9421 cgaccaaccg cccggtgcgg caccgagatc caccaccgtc attcccggtt tgaataaacg
     9481 gtcggtttgt tgaatttcat ctaacttaaa atacgcccgt gaacgcaatt tttgtttatg
     9541 tgctttttgc acaaattgat ctttaaaatg ttctgcaagc caacgtgatg aactcgcact
     9601 tcgttttcta cccatttgtt cttctatctt aattaattca acgatttata ataagggtta
     9661 aattagcaaa ttcaagagat gttctaaaat ttagctcggc ttattatgct taattaa
//
LOCUS       DANLRK010000098         9595 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.098, whole genome shotgun sequence.
ACCESSION   DANLRK010000098 DANLRK010000000
VERSION     DANLRK010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9595
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.098"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(<1..557)
                     /locus_tag="PXA33_001696"
     CDS             complement(<1..557)
                     /locus_tag="PXA33_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121010.1"
                     /translation="MAKQIFKQAPLPFVGQKRMFLAQVSQILNENITDDGQGWTIIDV
                     FGGSGLLAHTAKHIKPKAHIIYNDYDGYAERLKHIPDTNRLRKQIYDIIGKSTPKNKR
                     LDPDKKSQVINIIQSFDGYIDVNCVASWLLFSGQQINSLEDLFNKIFWNGVRQTDYPS
                     AEGYLDGIEVTHESFHKLLPRFQHK"
     gene            complement(604..729)
                     /locus_tag="PXA33_001697"
     CDS             complement(604..729)
                     /locus_tag="PXA33_001697"
                     /inference="COORDINATES: protein motif:HMM:NF021626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Com family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL5121011.1"
                     /translation="MQSLRLIEIRCKYCQKLLAKAKDVQHLEIKCVRCKSINKFN"
     gene            complement(876..1085)
                     /locus_tag="PXA33_001698"
     CDS             complement(876..1085)
                     /locus_tag="PXA33_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121012.1"
                     /translation="MFRNELQVIDGKRYIVLECQFRREWRVAIESRGTVTNGEAIEIC
                     QYWVKYKGVKPEQLKIVEVPDILKE"
     gene            complement(1088..1381)
                     /locus_tag="PXA33_001699"
     CDS             complement(1088..1381)
                     /locus_tag="PXA33_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121013.1"
                     /translation="MSESFSLEKALWEEPRPRLQLDLPCPVIKLEVGKRYYTINIHGV
                     DCYDGDMSFTEIDEWTYESIDDVQTKSRAEQGLLFASEADAQEWIDAMRNSSR"
     gene            complement(1374..1811)
                     /locus_tag="PXA33_001700"
     CDS             complement(1374..1811)
                     /locus_tag="PXA33_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121014.1"
                     /translation="MKTTQHILDEREQQHGNYDSFAKIYGGLRKVSDSHAEKLTWPQQ
                     IAVEMMLFKIARILNNGANHQDNYQDIAGYAMLGGGLYNPNVSAEVKALPKPLTDSIY
                     PESHLNKSSVWRLDLEFETKEEATEVLEALTGKSAGVSEVNNE"
     gene            complement(2050..2727)
                     /locus_tag="PXA33_001701"
     CDS             complement(2050..2727)
                     /locus_tag="PXA33_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein/antirepressor Ant"
                     /protein_id="HDL5121015.1"
                     /translation="MSNQVQFPVFNFNSSAVRVIIDPNQEPWFCGADVCRILGYVNES
                     LTLQKHCKENGVSKRYLTDKMQRQQKAIFINEPNLYRLIIKSRKPEAEKFEAWVFEEV
                     LPQIRKTGKYALQNNQQNLPLAPPPKKYSFDFTEDELQSLIWAWFAFVRGIHTFRYIY
                     PMFQKLGSNIAPEIYGQGFEYSHTAQSAHKILERITREFDCDPMTNWRVLKHLRGFDP
                     AFKKPTF"
     gene            complement(2975..3814)
                     /locus_tag="PXA33_001702"
     CDS             complement(2975..3814)
                     /locus_tag="PXA33_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HDL5121016.1"
                     /translation="MANSVMIVQGVEVKVTARDGEDYISLTDMCKAFGDSDQLIKNWL
                     QNKNTIEFLQVWEELNNPNFNLVELHQIKNNIGLNRFVMSVKKWTATGAIGLVAKAGR
                     YGSGTYAHKDIALEFGSWLSPEFKLYLIKEFQRLKQQEANESKLEWSVKRILSKANYR
                     IHTDAIKAHIIPALLNTKQHTFVYSSEADILNQALFGQTAKQWKDRNPNLKGNMRDHA
                     TVEQLTVLAALESQNALLIEQGYSQEERLAMLNRLAIQQMSSLLQTRAIEELKEKPLL
                     IEE"
     gene            4044..4421
                     /locus_tag="PXA33_001703"
     CDS             4044..4421
                     /locus_tag="PXA33_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121017.1"
                     /translation="MFSLLRDALTFAAMFTGILFPAIIHKYDLSLGTQIQLLSFVPAF
                     FVLCHFLIKRKYQRDFDRLSYLSAVVCVAMCAGVGAFSQSEMIELGFKVERVLDYVTI
                     KFLLYFWSLLLLPVVLRKLFGKD"
     gene            4553..4741
                     /locus_tag="PXA33_001704"
     CDS             4553..4741
                     /locus_tag="PXA33_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121018.1"
                     /translation="MKELFKQWLINQDSPFINSCGVECILSKVDDWLNIINANEEETE
                     TLVEWLNAFLQEVSVFVA"
     gene            complement(4745..5242)
                     /locus_tag="PXA33_001705"
     CDS             complement(4745..5242)
                     /locus_tag="PXA33_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HDL5121019.1"
                     /translation="MQQLIKNIEQWAEDRNLINGSTPQKQMLKLMEEFGELCAGVATN
                     EFETILDSLGDCLVICTIMRKQLKMEKLFSISHCDNTNFNCGVAAQIVQANIDLGNVA
                     SELSRGCDLSRLEDYLSGFENQLDLIAQFYKTDVQTALLYAYIQIKNRKGKMIDGVFV
                     KAEDL"
     gene            complement(5226..5426)
                     /locus_tag="PXA33_001706"
     CDS             complement(5226..5426)
                     /locus_tag="PXA33_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121020.1"
                     /translation="MTDTKRLDFIKKHKMEIRYNGLSDEFVISNHAENSQHFRMARNA
                     DLRTAIDEAMKEMRGLNNATTN"
     gene            complement(5423..5941)
                     /locus_tag="PXA33_001707"
     CDS             complement(5423..5941)
                     /locus_tag="PXA33_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HDL5121021.1"
                     /translation="MNEDKFFSVDVSNDIHILNLHETLEQAKQSCLNGATEAYEFAGD
                     MDDHESYEAYDLPYAVYGVVLGRAKSDIRPLTDEERESELFGEVEQVIEPPTLVENNG
                     WISIEDKLPPIETDVLGLCDISGMQLILIVSRELADNEWYFLSVNQYGLDDDVIAVTH
                     WQPLPKLPKDNQ"
     gene            complement(6051..6854)
                     /locus_tag="PXA33_001708"
     CDS             complement(6051..6854)
                     /locus_tag="PXA33_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdQ family protein"
                     /protein_id="HDL5121022.1"
                     /translation="MDKSTIQQISTLAVAATKEVQTDFGTVMLPEGIKLQNLEPFQAH
                     RNQFRAAFSTQRFGSLIEYATANAQENAQCFIDQEKMSAEIVFDMGNREQAGHAKHRA
                     NLVMKKTAAYKALCEINGSRRSQRDFSDFLEDWGGYLTAYHHEDEISIKNAVQAVRKM
                     TIDYARNEEHELSDFAAKKSAMESVEAKSTLQLPTHLVFTCNPYNGLDTRSFTLRVQV
                     LTGSGEPVLTARLVQAEQIEEAIATEFAEKLSDALSETSIKVNIGTIEI"
     gene            complement(6924..7283)
                     /locus_tag="PXA33_001709"
     CDS             complement(6924..7283)
                     /locus_tag="PXA33_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121023.1"
                     /translation="MAKTNVPEFLDELDCGIFKDKLATALSEVALGVLTHDKKGKVTV
                     EFSLDKMDSDSPSVQIQHKLSYVKPTKRGKSAEEDTTATPMYVHKGGALSATPEKTET
                     AVKESPSGLKALSKAAA"
     gene            complement(7319..7777)
                     /gene="ssb"
                     /locus_tag="PXA33_001710"
     CDS             complement(7319..7777)
                     /gene="ssb"
                     /locus_tag="PXA33_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDL5121024.1"
                     /translation="MAGINKVIIVGRLGNDPELRTMPNGDTTAKISVATSTEWTDKVS
                     GDKKQATEWHSIIAFRNLADIIGKYLKKGSQVYVEGKLRTRKWQAQDGTDRWTTEIIA
                     DQLQMLGSSSSGNSNNNWATEPAGNPPPTNPYNHVMTDSESRDFDDDIPF"
     gene            complement(7780..7977)
                     /locus_tag="PXA33_001711"
     CDS             complement(7780..7977)
                     /locus_tag="PXA33_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121025.1"
                     /translation="MARKIDAGVEIVEVDKLFIAKFFYDGKLQHTTYPQYSRQNAIML
                     INRKIERFNVGRDKPIKLYKE"
     gene            complement(7961..8146)
                     /locus_tag="PXA33_001712"
     CDS             complement(7961..8146)
                     /locus_tag="PXA33_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121026.1"
                     /translation="MNKMKQLAPFALLILLLGIVGRMDYDDHIQMQRYKCERNQGVWQ
                     VESNGNQYCGGKYGTKN"
     gene            8360..8635
                     /locus_tag="PXA33_001713"
     CDS             8360..8635
                     /locus_tag="PXA33_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121027.1"
                     /translation="MKNNILITYDLNKSGQNYAALIEKIKTLGAWAKVQQSAWYLHTS
                     YSSKEVLEMLSTVTDYNDSLFVAQLSEANWRGLSNEVSKFIQEQWWK"
     gene            complement(8638..8889)
                     /locus_tag="PXA33_001714"
     CDS             complement(8638..8889)
                     /locus_tag="PXA33_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121028.1"
                     /translation="MKNKNFEIAKVVILNEQLGSPKDLELKVVENGLSDEDKYHIKQA
                     VLKAVENGCLDPEMLASRCCSAIETINKSGINTGNGSCE"
     gene            complement(9342..9572)
                     /locus_tag="PXA33_001715"
     CDS             complement(9342..9572)
                     /locus_tag="PXA33_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121029.1"
                     /translation="MNAQATLNNHKDYILCGRKEKRTSDFINVFEVFENETTQEFVIE
                     RAMFRNGKLIDWNQSDKMNAEQAQQLWQAYIH"
BASE COUNT         2500 a         1938 c         1866 g         3291 t
ORIGIN      
        1 tctttatgct gaaaacgtgg taatagttta tgaaaacttt cgtgagtaac ttctattcca
       61 tctaaatagc cttccgcact tggataatcg gtttggcgaa caccattcca aaatattttg
      121 ttaaatagat cctctaaact attaatttgc tgtccgctaa atagcaacca agaagccaca
      181 caatttacat caatataccc atcaaaagac tgaataatat tgataacttg agatttctta
      241 tcaggatcta atcgcttgtt tttaggcgta ctcttcccaa taatgtcata aatttgcttg
      301 cgtagcctat ttgtatcggg aatatgcttt agccgctccg catatccatc ataatcattg
      361 tagatgatgt gagcttttgg tttgatatgt ttggcagtat gagctaataa cccactacca
      421 ccaaatacat ctatgattgt ccaaccttgt ccgtcatcag taatattctc atttaaaatt
      481 tgagaaacct gagctagaaa cattcgtttc tgacctacaa acggaagtgg ggcttgttta
      541 aagatttgct ttgccatagt tctcctatgg cgttcaggcg ttcttaacgc tcagacactc
      601 aattcagtta aatttattga ttgatttaca acgtacacat ttaatttcta aatgttgtac
      661 atctttcgct tttgccaata atttttggca atacttacag cggatctcaa ttaatcttaa
      721 tgattgcata taaatacact tttacttcat tttttgataa aattccgcct acctagctag
      781 gtggcggcgt atggctatat gcaagcgtgc tttgcgtagc tggcatttta agtgttaccg
      841 cacttataat gtcgccgttt ttaactcatt tgtccttatt ctttcagaat atctggcact
      901 tcaacaattt tcagttgctc aggttttact cctttatatt taacccaata ttggcagatt
      961 tcgattgctt ctccattcgt tacagtacct ctagactcta ttgcaactct ccattcgcgg
     1021 cgaaattggc attcaagtac gatataacgt ttaccatcta ttacttggag ttcatttcta
     1081 aacatagcta cctactacta tttcgcattg catctatcca ttcttgagca tctgcttcac
     1141 tggcaaatag taacccttgt tcagctctgc tttttgtctg cacatcatca atactttcat
     1201 aagtccattc atcaatttcg gtgaagctca tatctccatc ataacagtca acgccatgaa
     1261 tattgattgt ataatagcgt ttaccaactt caagttttat aacagggcaa ggtaaatcta
     1321 attgcaatct tgggcgtggt tcttcccaca gtgctttttc taaactaaat gactcactca
     1381 ttatttacct cactaacacc ggcactttta ccagttaaag cttccaacac ctcagttgcc
     1441 tcttctttgg tctcaaactc caaatccagc cgccaaacag agcttttatt aaggtgtgat
     1501 tcggggtaaa tactatcggt taatggtttt ggtaatgctt taacttctgc cgacacattt
     1561 gggttataaa gtcctccacc taacattgca taaccggcaa tgtcttgata attgtcttga
     1621 tgatttgctc cgttatttaa aattcgggca attttgaata acatcatctc aacggcgatt
     1681 tgctgtggcc aagtgagctt ttctgcgtgt gagtcgctga ctttgcgtaa accaccataa
     1741 atcttggcaa aactgtcgta gttgccgtgt tgttgttcac gttcatctaa aatgtgttga
     1801 gttgttttca tcgtgaattt tcctttagaa atagccgctt gcggaacaag cggttgggtt
     1861 aattagcgtt gagccataca aattttgcca tcgcagtcgt tgcgtaactc tagatggtca
     1921 gcagcaactg caaaggcggt taaaaagagt atgatttttg cgtatttcat cgtctaatcc
     1981 tcaaaatttg ggtgtggaaa tccgccgctt gatgtttcgc aagcggtcgg gttttataat
     2041 tttttgtgat tagaacgttg gctttttaaa tgccgggtcg aaaccacgaa ggtgtttgag
     2101 tacacgccaa ttcgtcattg ggtcgcaatc aaattctcgg gtaatccgtt caaggatttt
     2161 atgagccgat tgtgcggtgt ggctatattc aaaaccctgt ccgtagattt caggtgcgat
     2221 atttgagccg agtttttgaa acatcgggta gatatagcgg aaagtgtgaa tgccacgcac
     2281 gaaagcgaac caagcccata tgaggctttg gagttcatct tcggtaaagt cgaaagagta
     2341 tttctttggc ggtggtgcaa gcggtaggtt ttgctgatta ttttgcaacg catatttacc
     2401 agtcttgcga atttgtggca gaacttcttc aaatacccac gcttcaaact tttccgcttc
     2461 tggtttgcgt gatttgataa tcagtcggta taggtttggt tcgttgatga agatcgcttt
     2521 ttgttgtctc tgcattttat cggtgaggta tcgtttcgat accccatttt ctttacagtg
     2581 tttttgcaga gtaaggcttt catttacata acctaaaatt cggcaaacat ctgcaccgca
     2641 aaaccacggt tcttgatttg gatcgataat gacacgaaca gcagatgaat tgaaattgaa
     2701 aacagggaat tgaacttgat ttgacatttt ttgtaccttt tggatttaag tatttaatcc
     2761 gatcattaag tgggtgatcg gggttcaact taccctccaa aagatgggcg gagcttattc
     2821 agatgtattg tatatcgctc tctcgccccg atcatttcgg attaaaccta aaatttaggt
     2881 acaaaaaaac cgctttatat cggaggcgga aaccgctttt ggaaagtaag tgtggttatc
     2941 ttagccgaaa gggcggtttt ttgtcaatta gattttattc ttcaattagc aatggctttt
     3001 ctttcagttc ttcaatcgct ctggtttgta acagcgagct catttgctga atagccaaac
     3061 ggttgagcat tgccagtcgc tcttcttggc tgtaaccttg ctcaattagt agggcgtttt
     3121 ggctttccaa tgcagcaagg acggtaagtt gctcaacggt agcgtggtct cgcatattgc
     3181 ctttcaggtt cggattgcgg tctttccact gttttgccgt ttgtccgaag agagcttgat
     3241 tgagaatgtc cgcttcgctg gaataaacga aagtatgttg ttttgtattc agcaatgccg
     3301 gtatgatgtg agctttgatg gcatcagtat gaatgcggta gttcgccttg cttaaaatgc
     3361 gttttacgct ccattcgagc ttactttcgt tcgcttcttg ctgtttgagt cgttggaact
     3421 ctttgatgag ataaagctta aattctggac tgagccaaga accaaattcg agagcaatat
     3481 ctttgtgggc gtaagtgccg ctaccatatc gtcctgcttt tgctactagc ccaatagctc
     3541 ccgttgcagt ccattttttg acagacatca caaagcggtt taagccgatg ttgtttttaa
     3601 tttggtgtaa ttccaccaaa ttaaaatttg gattattcag ctcttcccaa acttgaagaa
     3661 attcaatggt atttttattt tgaagccagt ttttaatgag ctggtcactg tctccaaaag
     3721 ctttgcacat atcagttagg ctgatgtaat cctcaccatc acgagcggtt actttgacct
     3781 ccacgccttg tacaatcatt acgctatttg ccatttcgtt tctccgtttt taggtacaaa
     3841 aaaagccgtt ggtggacggc ttgaaagtgc ggttatctta atccgaattg ggggcggtgt
     3901 caattagatt aatccgtata agtatggaac caagttaaag agtagaacta caacacataa
     3961 ccaaaaaatg aaattcatat cttgctcagt tttgattttg tgtaaaatac gcttaagttt
     4021 gtttttcatt taggaaacct ttaatgttta gtttattgcg tgatgcttta acctttgctg
     4081 caatgtttac agggatttta tttcctgcaa ttattcataa atacgatcta tcacttggta
     4141 cacaaatcca attacttagc tttgttccag ctttctttgt gttgtgccat tttcttatta
     4201 agcgaaaata tcaacgtgat tttgacagac tttcgtattt atctgctgtg gtttgcgtag
     4261 caatgtgtgc aggcgttgga gcatttagcc aatcagaaat gattgagcta ggttttaaag
     4321 ttgagcgtgt tcttgattat gtgactatca aattcttact ctacttttgg agcttgctgt
     4381 tattgccagt tgtgctaaga aaattgtttg ggaaagatta gttggttatt taaagagagc
     4441 tatttgtcaa acctcaatat ctatttttgc gatctgcatc gaaaaaataa attttcgttc
     4501 tgacagtaaa acgctttcgt agtaatctca taaaaaacat aaggggaaca ctatgaaaga
     4561 gcttttcaaa caatggctaa tcaatcagga ctcgccattc attaatagct gtggcgttga
     4621 gtgtattttg tcaaaagttg atgactggct aaacataatc aacgccaacg aagaagaaac
     4681 cgaaacgctt gttgagtggc taaatgcttt tctgcaagag gtttctgttt ttgtggctta
     4741 ggtactataa atcttctgct ttcacaaaca cgccatcaat catttttcct tttcgatttt
     4801 tgatttgaat ataagcatag aggagagctg tttgtacgtc tgttttgtag aattgagcga
     4861 taagatcaag ctgattttca aagccgctta agtaatcttc taatcgagat aaatcacaac
     4921 ctctagataa ctcacttgct acattaccta aatcaatatt ggcctgaaca atctgagccg
     4981 caactccaca attaaaattg gtattatcgc aatggctaat gctaaacagt ttttccattt
     5041 tgagctgttt acgcataatc gtacaaatca ctagacagtc acctagactg tctaaaatag
     5101 tttcaaattc attggttgct acacctgcac ataattcccc aaactcttcc attagtttta
     5161 acatttgctt ttgtggggta gagccgttaa ttaaattgcg gtcttccgcc cattgttcga
     5221 tgtttttaat tagttgttgc attgtttagt cctctcattt ctttcattgc ttcatcaatc
     5281 gccgtgcgta aatcggcatt tcttgccata cggaaatgct ggctgttttc agcgtgatta
     5341 ctaataacaa attcgtctga taagccattg tagcggattt ccattttgtg ctttttgata
     5401 aaatctaagc gtttggtgtc tgtcattggt tgtcctttgg cagctttgga agtggttgcc
     5461 agtgggttac tgcaatcaca tcgtcatcaa gcccatattg atttacacta agaaaatacc
     5521 actcgttatc ggcaagttct cttgaaacta ttaaaattaa ctgcattcct gaaatatcgc
     5581 ataaccctag cacatccgtt tcaataggtg gtaatttgtc ctcaatggat atccagccat
     5641 tattttctac aagtgttggt ggttcaataa cttgttcaac ttccccaaat aactctgatt
     5701 ctctttcttc gtcggttagt gggcggatat ctgattttgc tctacctaaa acaacaccat
     5761 aaactgcata gggtaaatca taggcttcat agctttcgtg atcatccatg tcacccgcaa
     5821 actcataggc ttcagtagca ccatttaaac agctttgttt tgcttgctct aaggtttcgt
     5881 gcagatttag gatatgtata tcatttgata catccaccga aaaaaatttg tcttcattca
     5941 ttttctttct ccaaaatacc aatagttgag agaataataa ccgcttgtat tcaacaagcg
     6001 gtcaattttc attaaaaact tacaagtaaa cttataagct aacttacaag ctagatctca
     6061 atcgtaccga tattgacctt gattgatgtt tcgctaagtg catcggatag tttttccgca
     6121 aactcagtcg caatcgcctc ttcaatttgc tcagcttgaa ctaagcgggc ggttaatacc
     6181 ggctcaccgc taccggttag cacctgtaca cgcagagtaa atgatcgagt gtctaagccg
     6241 ttatatgggt tgcaagtaaa tacaaggtga gttggtagtt gcagtgtaga tttagcttcc
     6301 accgattcca ttgctgattt tttggctgcg aaatcgctga gttcatgttc ttcgtttcgg
     6361 gcgtagtcaa tcgtcatttt acgcaccgct tggacggcat ttttaatact gatttcatct
     6421 tcgtggtggt aagcggtcaa gtagccgccc caatcttcga ggaaatcaga aaaatcacgc
     6481 tgtgagcggc gtgagccatt aatttcgcat agagctttgt aggctgcggt tttcttcatc
     6541 accaaattag cacgatgttt agcgtgaccg gcttgctctc ggttgcccat atcgaataca
     6601 atttccgcac tcattttctc ttggtcaata aagcattgtg cgttttcttg ggcattggcg
     6661 gtagcatatt caatcaggct accaaaacgt tgcgttgaaa acgctgcacg gaattgatta
     6721 cggtgagctt ggaaaggctc taaattttgt aatttaatgc cttcaggtaa cattactgtg
     6781 ccaaaatcag tttgaacctc tttagtcgca gctacagcaa gagtagagat ttgttgaata
     6841 gttgatttat ccattttgag ttcctttttg tttaagtaaa aataaagcca cgcattaacg
     6901 tggcttggat tgaaaataat tgattatgca gcagctttag agagagcctt taaaccgcta
     6961 gggctttctt ttactgccgt ttctgttttt tcaggtgtag cagataatgc cccaccttta
     7021 tgaacataca ttggtgtggc ggtagtatct tcttcggctg acttaccacg tttggtcggt
     7081 ttgacatagc tcaatttgtg ctgaatttga acagatgggc tgtcgctatc cattttgtct
     7141 aagctaaatt caaccgtaac tttccctttc ttatcgtggg ttagtacgcc taacgccact
     7201 tctgaaagag ctgttgccag cttgtctttg aaaataccgc aatctaattc atctaagaat
     7261 tcaggtacat ttgtttttgc cattttgggt tctcctaatt agggtttgaa taaaattatt
     7321 agaacggaat atcatcatca aaatctcgtg attctgaatc cgtcattacg tggttatatg
     7381 gattggttgg cggtgggttt cctgccggtt cggttgccca attattattg ctgtttccgc
     7441 tgcttgagct accaagcatt tgtagctgat cagcgatgat ttcagtcgtc cagcggtctg
     7501 tgccgtcttg ggcttgccat ttgcgagtac gtagtttgcc ctcaacatag acttgtgagc
     7561 cttttttcag gtatttgcca ataatgtcag ctaaattccg aaaggcgata atgctatgcc
     7621 actctgtggc ttgtttttta tcgcctgata ctttatctgt ccattcggtc gaagttgcta
     7681 cgctgatttt ggcggttgta tcgccgtttg gcattgttcg caattccggg tcgttaccta
     7741 ggcgaccgac aataatcact ttgttaattc ctgccattgt tattccttat aaagtttaat
     7801 tggtttatct ctgccaacgt tgaaccgctc aattttgcgg ttaatcaaca taattgcgtt
     7861 ttggcggctg tattgtggat aggttgtgtg ctgtaatttg ccgtcataaa aaaacttggc
     7921 gataaatagt ttatctacct caacaatctc aacaccggca tcaatttttc gtgccatatt
     7981 tccctccgca gtattgattg ccattactct caacttgcca aacgccttga tttcgttcgc
     8041 atttgtaacg ttgcatttgt atgtggtcgt cataatccat tcgcccgaca atgcctaata
     8101 gcaaaattag caaggcaaat ggggcaagtt gtttcatttt gttcatagga ttgtccttta
     8161 gggttaaaaa aagcccacgt tttacggtgg gcaaatggag cgacttgcgt ctagttgtgg
     8221 gtattccgga acacacttgg tgaaatatgc tttggaataa gtgcctttct ttatacttgt
     8281 aaggctcaag tcccttattg cctctcacaa ctctaaggaa tataatcaag gctctcacaa
     8341 ctcaaataag gattaagtta tgaaaaataa tattttaatt acttatgatt taaataaatc
     8401 agggcaaaat tacgcagcac tcattgaaaa aattaaaact cttggagctt gggctaaagt
     8461 acagcagtca gcttggtatc ttcatacttc atattcctca aaagaggttt tagaaatgct
     8521 aagcaccgta actgattaca atgactctct ttttgttgct caattatccg aggctaattg
     8581 gagaggatta tccaatgagg ttagtaaatt tattcaagaa caatggtgga aatagaccta
     8641 ttcgcaactt ccattacctg tatttattcc gcttttattg attgtttcaa ttgcagaaca
     8701 acatcgtgat gcaagcattt ctgggtccaa gcaaccattt tctacagctt taagtacagc
     8761 ctgtttgata tggtatttat cctcatcaga caggccattc tctacaacct ttaattccaa
     8821 atctttaggg cttcctagtt gttcatttaa aataacaact ttcgcaattt caaaattttt
     8881 gtttttcata attacctcgt gtttaaatta ttccaaaacc cactcataat aggctttgga
     8941 ataaatgcgt gtctgcccac gccgtcgccc gccctgcatt tcgcctgttc agtcgggtgc
     9001 tgcttgtatc cgtacttact caaacttgag ataagcgtga ttgcttagga ctgataccag
     9061 tgttgccttt tcagctctcc acaccttgcg agtgtgttgc ctaaaccttg ccacctctgt
     9121 ttggcaagtc gttcaaattt gtatgtcagg gtctaaccaa ccttgccgag tgataaagca
     9181 ttactttaca actgctcgag gttgttaata tcgtccgcac aagcactaaa ttgttaaaga
     9241 acatcccaca gtctctgctt atctctcggc tattgcctgc tatgtgggct aggtctagaa
     9301 cctttattca agccctcagt aaagggcttt gataaaaatt cttagtgaat gtaggcttgc
     9361 caaagttgtt gagcttgttc tgcgttcatt ttgtcgcttt ggttccagtc gattaacttg
     9421 ccatttctga acattgctct ttcaatcaca aactcttgcg tggtttcatt ttcaaagacc
     9481 tcaaacacat tgatgaaatc actagtgcgt ttttctttac gtccgcataa aatgtagtct
     9541 ttgtggttat ttagcgttgc ttgtgcgttc attttggttt cctctttgtc tgtga
//
LOCUS       DANLRK010000099         9587 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.099, whole genome shotgun sequence.
ACCESSION   DANLRK010000099 DANLRK010000000
VERSION     DANLRK010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9587
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.099"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            3..>9587
                     /locus_tag="PXA33_001716"
     CDS             3..>9587
                     /locus_tag="PXA33_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
                     /protein_id="HDL5121030.1"
                     /translation="MNKHCFRTIFSKTLQRIVVVSELAKLAGKSESESMTSGAILQKI
                     CKIRPLAFSLFCALGFVTLSDNAMAETLIIQADSTAPKNQQPIILQTANGLPQINIQT
                     PNDKGLSHNKYSQFDVAEKGAILNNSRTNTATQQAGMVQGNPYLARGEAKVILNEVNS
                     SKPSVMKGYVEVAGKKAEVIIANPSGLHCDGCGIINADRATLTTGKPQINQGNLEGFV
                     VEKGKVTVAGKGLDNSRVDYTDILAREAKINAGVWSKKETKVITGKNTVKRSNSDNNL
                     QIIHTNQPLAGEEKPTVAIDVGELGGMYAGKIHLIGTEQGVGVRNAGHIGASADTLTI
                     DSQGRIVNSGTFNAQRQVQLNAQHGIDNQGKIENKQGNIQLNSKADIQNSGSIVARGG
                     NIQKKAETKIQQSGETVAKGNITYTARQIHADKNSLIAAGVDVTDTAQGEVRKLEAQS
                     VAGKTIQLTAPEKTTVQGKNIASGKINVAAAEIDANQSQNNAHTVEYSAKSGDIQANS
                     AQFSANQIKLTTPKWLSTEGSQLTAEVIEAQQANLNARGSVWKQTGEQDFRLKAESIH
                     TDSARFSVGGHFEIDGNRLTNNQGLLSSGKRLGLNLNDAVDSVDGKLVAKGELSIRAP
                     LLNNQQGLILSGEKQAITVNTLKNQSGVITSQTEQTLDITSELQNQGGRISASGVAIN
                     AQSLDNREQGEISSVKELALTVSQQVDNRHGVIQSVGNLTLNSTLLDNQGGVLKSATD
                     ITLNVPTVLNNRISENGSLIEAGGSLTMNSFEVENQGTLARQPQPIQGIIASQFVLNS
                     TKQLNNEKGGIYIGSSARFTLSDSLNNRNGEILSWGDLAIFGGKSLTMTNTEGKVSAN
                     NGLEITAKALSGDGDVQAGDIRLNLQENFDITKDINANRSMIIQTEGDILNRKKLAAN
                     DRLQLTANQITNAQNARISSADTRLTATSTVYNEGLINSRSDNDNAQTVIKAQEIHNI
                     GTGNIYGDKVALGAERILNQDKDGTSATIAARQSLGLAAKVIENNTLVYEANKKSGSL
                     IYSGGEIAIGRQLNEQDTVIGNAGQLHNRSSIIEAEGRIQLNADKVANVNSHYRSELE
                     TTDRQTVNEHFILPRDKHNRGGSDVYDNGHLPTINVNQLRKGYYRKTWSYWQKYDEAN
                     LPVITDISQLNATTVLAKPYAQSCANDESVDCTLLPAGNYKKDNPVWTWFNLTPPAED
                     APEMTPEIIQAMNEEDLANQHLSEAEQEKLQKEQGQGIAPVVPARPIEPVRGSTETEA
                     QYQKRLQTYQAELKAYEAAEAAYQRYVLLKPLIDWEAKYGANIQALDEAIAKHNKANL
                     SQEYPHFWNLWIKERMVKENITKASLPAQILAGGDLTYQSDEFINDKSWVIAGKALNP
                     IGRSRIANLDDADAVRQDWELGRRQFSFTEYHGGTRRRHSRYDENDGPLARLKEVHKD
                     MNIFVELENTSPTAYTDYVDAKAANAVKPEGNQVERGNLPDAPNPPTMAIERIPSPNA
                     AAFEVRSMTVDTRLPNQGLYRINPQADSHVLIETDPDFTDKKRWLSSDYMFNALRHDH
                     NNVHKRLGDGFYEQRLIREQINRLTGRQFLGDYTDFDSQYKALMDAGITFAQKFNLRL
                     GITLSPEQVASLTSDIVWFETQTVRLPSGETVSVLVPKVYALARKGDIDGKGSLISAD
                     ALTINSKEIVNQGTIAGRRLAQFNAERLENSGTLSGGVLAAKVSGDLENIGGKIEADK
                     AILLDIAGNFNHTSTTRTTQIDLDGFKRAETNLDRKALLHVKDENGLLQVQAGNITSA
                     GADIINDGQGQTYLSAKNQLNLTALQVGFDEKMGGGNHYRNEALNDVVISRVQGKGDV
                     VVSGKNLYSEGADLDAKARLALLAENDMVLGSATRSRDYEEYHKTKSGGALAKKTKTT
                     YDNISETEHKGTELTGEDILVKAGHDIQGESLLAVSRSGDIDIIGGNNVTLSSATNQL
                     SEQHIRETKRSGFLNGGEIGFTIGSQKTRQENSLEGAIQSTARNTLGSEGGNIRIQAG
                     NKASVSNVDVLISNEKTAVISGKNGVRVESGKDVINSTDNYEFSQSGLSIALSTPVTD
                     AVQSARQSVQKAKATQNEKLKGVYAVKAAEDAVIAAQNAQKVAETLGNLGNALTENHA
                     AAENPAVKISVSLGTQKQQRESRSQSVTHSKSTLNGGNIALISGAGKVALEGVDTRVK
                     DTLLLDGKLGIESKGVADTYQNNTKNKNHSASVGVFIGLDRDSFGIGFEAGVSGGKGK
                     ENIETETWQNNQLQAGKIVTNSANGKLMLDATNVKANHWEGEVQDFEARSRQDISKYK
                     SEYTEGSVHGTLAVGTTGVNGHVAYNGAKLNTAQVENQTAIDIGKSGMDVKVKKNAHF
                     EGAVMTSQAEKENNRFQAGTLTTGDIENHSELKTRSVAISGGTSGVNPMSALSLLGNK
                     NESERSTTKAAIGENIAITLTEDLNAETTLNHLNRDTQNANQKVTKYDISEVKETQEL
                     VKGIGEIAEKAYQIYTHSEREKVTEAKLELGKAQAQKASKEEIGKLEANLDRLQKEFD
                     KNYGTGSKTKRAVEAVTAALQGLAAKDVGQAAVGLASPYLNAEIKKYTEGDTQANLIA
                     HALLGAVEAAATGNNVLAGAAAGAGSEAAADFIVKTLYKGKSTQDLTEAEKQNVALLS
                     QLASGLASGLIGDSTQSAAVGADIGKRAVENNYLSNWQEQKRSQELKSCHGDARCENQ
                     VRLYWGLVDIGQDVSFVSGAVAGVPENLAESVEGLISMFSSPIETIVAMKELIAREDT
                     LSYIANMTKQEYLAKLDKMQVYYEQAGAVGSFNAGKEFGNMMTDVVTALTGAGALVKT
                     GVGTTAKIVTKSGKELAIPKTRSSASLGTCSFHGDMEVKTDKGYRQISSIKVGDKVLA
                     KNERTGITTYQKVQAHYSNPYDYTVYVEVADSKGKYHTIVSNKIHPFFTQVLSGRVPI
                     SSEGHSYNGEIAKAQWVDAQHLQKGYRLLAESGEWQTVTKVKIKQERLKAYNMTVEKD
                     HTYFIRGTNADNEGVWVHNDCWHALPNDAKQVKNIDGYKAYQFTDPKDGKVVTVVQKD
                     LNRFETPNHQAGVDPHFNRLSQQIDAETGRYLSTDNPVDGLYNRAYLRSETLQKIYAN
                     YEKLPNGNYRNKLTRQEIEQPIDIGHMYGWEHRRLSLAAKELNWSQKQFNDYVNARPE
                     NFRLENMSENRSHKNEMPG"
BASE COUNT         3124 a         1952 c         2412 g         2099 t
ORIGIN      
        1 ttatgaataa gcattgtttt cgcactattt tcagcaagac cttacaacgt attgtggtgg
       61 tatctgaatt agcaaaatta gcaggtaaat cagaaagtga aagtatgaca agcggtgcaa
      121 ttttacaaaa aatttgcaaa attcgaccgc ttgcgttcag cttgttctgt gcgttgggat
      181 tcgttacctt atcagacaac gctatggcgg aaactttgat tattcaagcc gatagcactg
      241 ccccgaaaaa tcagcaaccg attattttgc aaaccgccaa cgggctgcca caaatcaata
      301 ttcaaacccc gaacgataaa gggctttccc acaataaata cagccaattt gacgtagcgg
      361 agaagggggc aattctcaac aacagccgca ccaacaccgc cacccaacaa gccggtatgg
      421 tgcaaggcaa cccgtatctt gcccgtggcg aagcgaaggt gattttaaat gaagtcaatt
      481 ccagtaagcc ttcggtaatg aaaggttatg tggaagtggc tggcaaaaaa gcggaagtga
      541 ttatcgccaa cccgagcggc ttgcactgtg acggctgcgg tattatcaac gccgaccgag
      601 ccacgctgac caccggaaaa ccacagataa atcaaggcaa tttagaaggt tttgtagtgg
      661 aaaaaggcaa ggtaacggtt gctggcaaag ggctggacaa cagccgagtg gattacaccg
      721 atatcctcgc ccgagaagcg aaaatcaacg ccggtgtctg gtcgaaaaaa gagactaaag
      781 tcattaccgg caaaaacacc gtcaagcggt caaattcgga caacaatttg caaattattc
      841 atacaaatca accgcttgca ggggaagaaa agcccacagt tgcgattgat gtgggtgagc
      901 tgggcggaat gtatgccggc aaaatccacc tgattggtac ggaacagggc gtgggcgtgc
      961 gtaacgctgg gcatatcggt gcaagtgccg atacgctcac cattgacagt caagggcgga
     1021 ttgtaaacag cggcacgttc aacgctcaaa gacaagtgca actcaatgcc caacacggga
     1081 ttgacaacca aggcaaaatt gaaaataaac agggcaatat tcagcttaac tcgaaagccg
     1141 atattcaaaa tagcggttcc atcgtggcga ggggtggcaa tattcagaaa aaagccgaaa
     1201 ctaaaatcca gcaaagtggc gaaacggtcg caaaaggcaa tatcacctac accgcccgcc
     1261 aaattcacgc cgacaaaaac tcactgattg ccgctggggt ggacgtaacc gacactgcac
     1321 aaggtgaagt acggaagctg gaagctcaat ctgtggcagg gaaaactatt caactcactg
     1381 ccccagaaaa aaccactgtg cagggaaaaa atatcgcttc ggggaaaatt aacgtggcgg
     1441 cagctgagat tgatgccaat cagagccaaa ataatgccca taccgttgaa tacagtgcaa
     1501 aatcgggcga tattcaggcg aatagtgcgc agtttagtgc aaatcagatc aagctcacca
     1561 ccccaaaatg gctttccact gaaggaagcc agctaaccgc agaggtgatt gaggcccaac
     1621 aagcgaattt aaatgctcgt ggctcggttt ggaagcagac tggtgagcag gattttcgcc
     1681 taaaagcaga gagtatccat actgactcgg ctcgcttttc agtgggtgga cattttgaga
     1741 ttgacggtaa tcgtttaaca aataaccaag ggctattaag tagtggcaag cgtttagggc
     1801 taaatttaaa tgatgctgtg gattcagtcg atggcaaatt ggttgcaaaa ggcgagttat
     1861 ctattcgtgc tccattgctg aataatcagc aaggtctgat tttgagtggc gaaaaacagg
     1921 cgattacggt caatacgtta aaaaatcaat cgggggtgat cacctctcaa acggagcaaa
     1981 cccttgatat aactagcgaa ctgcaaaatc aaggcgggcg aatttccgcc agcggtgttg
     2041 cgattaacgc tcaatcctta gataaccgtg agcagggaga aattagttca gtaaaagagt
     2101 tagcgctcac ggtttcccaa caagtagata atcggcacgg tgttattcaa agtgtgggaa
     2161 atctcaccct aaatagcacg cttttggata accaaggtgg ggtactgaaa tcggccacag
     2221 atatcacgct caatgtaccg actgtcttaa ataatcggat ctcggaaaat ggctcactta
     2281 ttgaagcagg tggctcactt acgatgaaca gtttcgaggt ggaaaatcaa ggtaccttgg
     2341 cacgccaacc tcagccaata caggggatta ttgcatcgca gtttgtactg aacagcacaa
     2401 aacagctcaa taatgaaaaa ggcggtattt atatcggctc atcagcacga tttaccctca
     2461 gcgacagctt aaataaccgt aatggggaaa ttttaagttg gggcgatctg gctattttcg
     2521 gcggaaaatc actcacaatg acgaataccg agggaaaagt ttctgcaaat aatggtttgg
     2581 agatcactgc caaagcgtta tccggtgatg gtgatgtaca agcaggcgat attcgcctga
     2641 atttgcaaga gaactttgat attaccaaag atatcaatgc gaataggtcg atgattattc
     2701 agactgaggg cgatattctc aatcgcaaaa aattagccgc aaatgaccgc ttgcagctca
     2761 cggcaaacca aataaccaat gcacaaaatg cccgaataag ctcggcagat acccgtttga
     2821 ctgcaacttc aaccgtctat aacgaaggct taatcaatag ccgctcggac aacgataacg
     2881 ctcaaactgt gattaaagct caagaaatcc acaatatcgg cacagggaat atttatggcg
     2941 ataaggtggc gttaggtgcg gagaggatac taaatcaaga taaagacggg acctctgcca
     3001 ctattgctgc tcgtcaatct ctcggtttag cggctaaagt aattgaaaat aataccctag
     3061 tttatgaagc gaataagaaa agcggttcat taatatatag cggaggagag atagcgattg
     3121 gacgtcaatt aaatgagcaa gataccgtta ttggcaacgc agggcagcta cataaccgca
     3181 gcagtattat tgaagctgaa gggcgtattc agttgaatgc ggataaggta gcgaatgtaa
     3241 atagtcatta tcgttcggaa ttggagacca ctgatcgcca aactgtgaac gaacatttta
     3301 ttttgccaag agataagcat aatcgaggtg gaagtgatgt atatgataac gggcatttgc
     3361 cgacaattaa tgtgaatcaa ctccgcaaag gctactatcg caaaacgtgg agctattggc
     3421 aaaagtatga tgaagcaaac ttgccggtaa ttaccgatat tagccagctg aatgccacga
     3481 ccgtattggc aaaaccctat gctcaaagct gtgcgaatga tgaaagtgta gattgtactc
     3541 tattacctgc aggtaattat aaaaaagata acccagtatg gacgtggttt aatttaacac
     3601 caccagctga agatgcaccg gaaatgacgc ctgaaattat tcaagcaatg aatgaagaag
     3661 atttagcgaa tcagcattta agtgaggcag agcaggaaaa attacagaag gaacaagggc
     3721 aaggcattgc accggtggtt ccggcaagac cgattgaacc tgttagaggc tctacggaaa
     3781 cggaagccca ataccaaaaa cgactacaaa catatcaagc ggaactgaaa gcctatgaag
     3841 cggcagaagc ggcttatcag cgttatgtgc tattaaaacc gttaattgat tgggaagcaa
     3901 aatatggtgc caatattcaa gcattagatg aagcgattgc aaagcacaat aaagcgaatt
     3961 taagccaaga atacccgcat ttctggaatt tgtggattaa agagcgtatg gtaaaagaga
     4021 atatcaccaa agccagctta ccggcacaga tcttggcagg tggcgatctc acttatcaaa
     4081 gcgatgaatt tatcaacgat aaaagttggg ttattgcagg caaagcgtta aacccgattg
     4141 gtcgcagtag aattgccaac cttgatgatg cagatgctgt tcgccaagat tgggaactgg
     4201 gaaggcgcca attctctttt actgagtacc acggaggcac aagacgtcgc cactcccgct
     4261 acgatgaaaa tgatggtcct ctcgctcgtt taaaagaagt ccataaggat atgaatattt
     4321 ttgtcgagct ggaaaatact tcaccgaccg cttatacaga ttatgtggac gctaaagcag
     4381 caaatgccgt gaagccggaa ggcaatcagg ttgaacgggg taatctaccc gatgccccta
     4441 atccgccaac aatggcgatt gaacgcatac caagcccgaa tgcagccgca tttgaggttc
     4501 gctcaatgac ggtcgatacc cgtttgccaa accaagggct ttatcgcatt aaccctcaag
     4561 cggatagcca tgttctgatc gaaaccgatc cggatttcac cgataaaaaa cgctggttaa
     4621 gcagtgatta tatgttcaat gccctacgcc acgaccataa caatgtgcat aaacgtttgg
     4681 gcgatggttt ttatgaacag cgcttaattc gagagcaaat caaccgctta accggcagac
     4741 aatttttggg cgactatacg gattttgata gccaatataa agccctgatg gatgccggca
     4801 tcacctttgc gcagaaattc aatcttcggt taggcattac cttatcgccg gagcaagtgg
     4861 caagcttgac cagcgatatt gtttggtttg aaacccaaac ggttcgcttg ccaagcggtg
     4921 aaacggtatc ggtattagtg ccgaaagtgt atgcccttgc acgcaaaggt gatattgacg
     4981 gcaaaggcag cctgatttca gccgatgcgc ttaccatcaa cagtaaagag attgtaaacc
     5041 aaggcacgat agcaggccgc cgtttagcac agtttaatgc cgaacggtta gagaatagtg
     5101 gcacactcag tggtggcgtg cttgctgcca aagtgagcgg tgatctagag aatatcggcg
     5161 gcaagattga agcggataaa gcgattttgc tggatattgc gggcaatttt aaccatacct
     5221 ccaccactcg aaccacacag attgacctag acggtttcaa acgcgctgaa accaaccttg
     5281 accgcaaagc cctactgcac gttaaagatg aaaatggact gctgcaagta caggcaggca
     5341 acatcacctc agcaggcgca gacatcatca atgacggaca ggggcagacc tacttatctg
     5401 caaaaaatca gttgaatttg accgctttac aggttggttt tgatgaaaaa atgggcggtg
     5461 gcaatcacta tcgtaatgaa gccttgaatg atgtggtcat ttcacgggtg caaggcaaag
     5521 gcgatgtggt ggtttcaggg aaaaaccttt acagtgaagg agcggatttg gatgccaaag
     5581 cgcgtttagc gttactggct gaaaatgata tggtgttggg tagcgcaaca cgttctcgcg
     5641 attatgaaga gtatcacaaa acaaaaagtg gtggtgcgct agcgaagaaa acaaaaacga
     5701 cttatgacaa tatttctgaa accgaacaca aaggcactga actaaccggt gaagacattt
     5761 tagtgaaagc cggtcacgat attcaaggtg aaagtcttct tgccgtctca cgcagtggcg
     5821 atattgatat tatcggcggt aataatgtaa cgctcagttc ggcaacaaac caactttcag
     5881 aacagcatat tcgtgagaca aaacgcagcg gtttcttgaa cggtggtgaa atcggcttta
     5941 ctatcggtag ccaaaaaaca cgccaagaga acagcttaga gggggcaatc caaagcacgg
     6001 cacgcaatac attgggcagt gaagggggca atattcggat tcaagccggc aataaagcga
     6061 gcgtgagcaa tgtcgatgtg ctgatttcca acgaaaaaac cgccgtgatt tcaggcaaaa
     6121 acggggtacg ggttgaatca gggaaagatg ttattaacag caccgataat tatgaattta
     6181 gccagtcggg cttaagcatt gcgttgagta caccggtaac ggatgcggtg caatcggcac
     6241 ggcaatcggt gcaaaaagcg aaggcaacac aaaacgagaa actcaaaggg gtatatgcgg
     6301 taaaagcggc ggaggatgcc gtgattgcag cacaaaatgc tcagaaagtg gcagaaacgc
     6361 tgggcaatct tggcaatgca ctcacagaaa accatgctgc ggcagaaaat ccggcggtga
     6421 agatttcagt cagtctcggc acgcaaaaac agcaaagaga aagccgtagt caaagcgtga
     6481 cccacagcaa aagcaccctg aacggcggca atattgcctt gatttcggga gcgggcaaag
     6541 ttgcgctgga aggggtggat acccgagtga aagacaccct gctgcttgac ggtaagctgg
     6601 gtattgaatc caaaggtgtt gcggatactt atcaaaacaa cactaagaat aagaatcaca
     6661 gtgcgagtgt gggcgtattt atcggtttag atagagatag ttttggtatt ggttttgagg
     6721 caggagtgag tggaggtaaa ggcaaggaga atatcgaaac tgaaacgtgg cagaacaacc
     6781 aactgcaagc gggtaaaatt gttacaaatt ctgcaaatgg taaactcatg ctggatgcaa
     6841 ccaatgtcaa agcaaatcat tgggaaggtg aggtacaaga ttttgaggca agaagtcgac
     6901 aagatataag caaatataaa agcgaataca cagaaggcag cgtacatggc acattggcgg
     6961 tagggacaac aggagtaaat ggtcatgtgg catataatgg agcgaagttg aacactgctc
     7021 aggtggaaaa ccaaactgca atcgacatag gcaagagtgg aatggacgtt aaagtgaaaa
     7081 agaacgccca ttttgaggga gcggtaatga ctagccaagc ggagaaagaa aacaaccgct
     7141 tccaagccgg cacgctgacc accggcgata ttgaaaacca cagtgaattg aaaacccgaa
     7201 gtgtcgcgat tagcggaggg acaagtgggg tgaacccgat gtcggcactg agcttactgg
     7261 gcaataaaaa cgaaagcgag cgaagcacaa caaaggcagc gattggagaa aatatcgcta
     7321 tcaccctcac cgaagaccta aatgcggaaa ctacgcttaa tcatctcaac cgagatacgc
     7381 aaaacgccaa tcaaaaagtg acgaaatacg atatcagtga ggtgaaagaa acacaagagc
     7441 tggtgaaagg cataggcgag attgcagaaa aggcttatca gatttacacg catagtgagc
     7501 gtgaaaaagt gacggaagcg aagctggaat tgggcaaagc acaagctcaa aaagcgagca
     7561 aggaagagat tggtaaatta gaagccaatt tagaccgctt gcaaaaagag tttgataaaa
     7621 actacggcac gggcagtaaa accaaacgag cggtggaagc ggtgacggca gccttgcaag
     7681 gtttagcggc gaaagacgtg ggacaagcgg cagtgggttt agcctcacca tacttaaatg
     7741 cagaaatcaa aaagtacacc gaaggggata cccaagccaa cctgattgcc cacgccttac
     7801 tgggtgcggt ggaagcggct gcgacaggga acaatgtact ggcaggggct gctgcaggcg
     7861 cagggagcga agcggcagcg gactttatag ttaaaaccct ctacaagggc aaatccacgc
     7921 aagacttaac cgaagccgag aaacagaatg tggcgttgct aagccagctt gcaagcggac
     7981 tggcttcagg tttgataggg gatagtacac aatcggcagc ggttggggcg gatattggga
     8041 aaagggcagt ggagaataac tacctctcga actggcagga gcagaaacgc tcacaagaat
     8101 taaaatcctg ccatggtgat gcccgctgtg aaaaccaagt tcgattatat tggggattag
     8161 ttgatattgg gcaagatgtt agttttgtat caggcgccgt tgctggtgtt cctgagaatc
     8221 tcgctgagag cgttgaagga ttgatctcca tgttctcaag cccaatagaa actattgtgg
     8281 cgatgaaaga attgatcgca agagaagata cgctctccta tattgctaat atgactaaac
     8341 aggagtattt agctaaactc gataaaatgc aggtttacta tgaacaggca ggggctgtag
     8401 gttcttttaa tgccggaaaa gagtttggca atatgatgac tgatgtggtg acagcattaa
     8461 ccggtgctgg tgctcttgtt aaaacaggtg tagggacaac ggcgaaaata gtcaccaaat
     8521 caggtaaaga acttgcgatt cctaagacca gatcgagcgc tagtttaggc acttgctcat
     8581 tccacggtga tatggaagtc aaaactgata aaggttatcg acagatttcc agcattaaag
     8641 tgggcgataa ggtgctggcg aaaaatgaac gcaccggtat aaccacctat cagaaagtcc
     8701 aagctcatta cagtaatcca tacgattata cggtgtatgt agaagtcgca gacagcaaag
     8761 gaaaatacca caccattgtg tcaaacaaaa tccacccgtt ctttacgcaa gtattatcgg
     8821 gcagagtgcc gatttcttcg gaggggcatt cttacaacgg agagatagca aaagctcagt
     8881 gggttgatgc tcaacatttg caaaaaggct atcgcttact tgctgaaagt ggtgagtggc
     8941 agactgtcac taaggtaaaa atcaaacagg aaagattaaa agcctacaat atgacggtag
     9001 agaaagatca tacttacttt attagaggta caaatgccga taatgaaggg gtatgggtgc
     9061 ataatgattg ttggcacgct ttaccgaatg atgccaaaca ggtaaaaaat attgatggct
     9121 ataaagctta tcagttcaca gacccgaaag acgggaaagt ggtgacagta gttcaaaaag
     9181 atctcaatcg ttttgaaacg ccaaaccatc aagccggggt agatccacat tttaaccgtt
     9241 tgtctcagca aattgatgcc gaaacgggac gctatttatc gacagataat ccggtagatg
     9301 ggctatataa ccgagcttat ttacgctcgg aaacgttaca gaaaatttat gctaattatg
     9361 aaaaattgcc aaatggtaat tatcgcaata aattaacccg tcaagaaatt gaacagccta
     9421 ttgatatagg tcatatgtat ggctgggaac atcgccgatt atcattagcg gcaaaagaat
     9481 taaattggag tcaaaaacaa tttaatgatt atgttaatgc gagaccggag aatttcagat
     9541 tagaaaatat gtctgaaaat cgtagccata agaatgagat gccagga
//
LOCUS       DANLRK010000100         9526 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.100, whole genome shotgun sequence.
ACCESSION   DANLRK010000100 DANLRK010000000
VERSION     DANLRK010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9526
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.100"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            <1..366
                     /locus_tag="PXA33_001717"
     CDS             <1..366
                     /locus_tag="PXA33_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121031.1"
                     /translation="LVQAELKYSGSNIPSEYSKFNLENVLIDFPNKTNSVAYQNLVIH
                     NAKTRAGKLKTQYANAHDLGNLVLVEDIQKIFSGLLEIIYQVRCHLVHGSLSPTPENH
                     EVVKYCYLILWDCLKGFCD"
     gene            complement(634..1371)
                     /locus_tag="PXA33_001718"
     CDS             complement(634..1371)
                     /locus_tag="PXA33_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL5121032.1"
                     /translation="MIKIENLTLAHGIQHLSCYLPKGKLIGIMGANGAGKSSLLKAIA
                     GILRPTSGEIWLENCKLHALSAVERSRQFAYLAQQLNVAWQLSVYDVIALGLPAPLPP
                     EQERQKVRSVAEQLAISHLLDNTYQQLSGGEQTRVQLARCLMKQAPILLADEPIAALD
                     PYYQIDIMQQLKALTPTQTCVVVIHHLPLAYRFCDEIILLKNGNLLVSGNTQAVLNAE
                     NIATAFGVKAEMDWEKREMWGIEKLEV"
     gene            complement(1368..2336)
                     /locus_tag="PXA33_001719"
     CDS             complement(1368..2336)
                     /locus_tag="PXA33_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDL5121033.1"
                     /translation="MKKLNIVLGSLLLLIVSNAAFYQLSDFALLKNAEGVLSDLRWLV
                     LGEIRLPRIGLAVLTGASLALAGNAMQGVFKNPLASPGLLGSSSGATAVSVFLLYYFS
                     APMGWLLFGGVAGALGAFLLVWLISFRHGTTMMILSGVAVNVLLGAAVTLLLSNAESP
                     WALAELYRWLQGSLVWATAEAVCWALPLILLGIVCLYRERRYLDLLTFGEETAATMGI
                     NLQRSFFTVSIGVALLVGATIPQTGTIGFIGLIAPHLARIWLKKPPSQLYLTSALIGA
                     LLLLIADLAVQYVPFFARIYIGTLTAILGVPFLIWILFTQQWRLAQ"
     gene            2517..2852
                     /locus_tag="PXA33_001720"
     CDS             2517..2852
                     /locus_tag="PXA33_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL5121034.1"
                     /translation="MYHIERTEIFDQWLENLKDPLAVVTIARRIERAESGNFGDTKYI
                     AEGISEMRLNVSKGYRLYYAQKGSIIYILLNGGHKGTQQADIDKAKMLWAEIKQHMAE
                     QEKKNERII"
     gene            2836..3159
                     /locus_tag="PXA33_001721"
     CDS             2836..3159
                     /locus_tag="PXA33_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative addiction module antidote protein"
                     /protein_id="HDL5121035.1"
                     /translation="MNELYDAPIPQATREKFGIQPFDAAEYLANDELIALYLAETLKD
                     GTDEEFIQALNTVARAKGMNELAKKAGIGRESLYQSLSSSKPRFETIRKIISALNLEL
                     SVVKA"
     gene            complement(3149..5035)
                     /locus_tag="PXA33_001722"
     CDS             complement(3149..5035)
                     /locus_tag="PXA33_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HDL5121036.1"
                     /translation="MTQLDSVTVYSAYATPINQDKTASSVTVLTEKDFAARNATYVSD
                     VLKTVPGVAIGQQGGRGTLTSLFLRGAESRHTAVVIDGVKVNPINIGNFDFGGLPISN
                     IERIEVLRGEQSALWGSSAMGGVVYITTKSGLYKEKPFNAEVDLGLGSNNTRDASATL
                     SGFHNGFYYALHGDSHRTKGISALSKNHFSYTTETGSEVKTGGASERDGFHRDNGSLR
                     LGYDLGNKGVEVLAAQSSQTVHIDGYNSDVSGEYSRTRNQTFKLGGYWGNEQELLKHQ
                     ANISQFNSKATHFGSNARYSNEKQLNANYQLDVNFDREGEVTQAVSLLTDYAKTRYTS
                     DKYLREKTLSEKSAALEYRLFTEQDHSFSISGRYTDNSQFKNSITGRISGAYRLSPNL
                     CSDRLLLELAEPQQIRAMSPYSQKPLMMLDKLNTDKPTVEPELTALLPYADSTILLNE
                     TFYPQLTARLKQLGFKLVALNDSPQTPEQLFTLILQLGELTQNQAKAEKLVERLRLQK
                     IPLKQPLAETLILSETGMIEPHFPQYQTLLDLLGLSPLKSDLTPQNFSLEKLLLAQPK
                     QLLFLTDNQSYNNQAELLKHPALQKIWQKMSQNPPLVLPMKYTYCFDHGVWQGIQLMH
                     KLTP"
     gene            5065..5865
                     /gene="metF"
                     /locus_tag="PXA33_001723"
     CDS             5065..5865
                     /gene="metF"
                     /locus_tag="PXA33_001723"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HDL5121037.1"
                     /translation="MQQKWRMLWDSIDRLSPLKPKFVSVTYGANSGERDRTHSIVKSI
                     QEKTGIISAPHLTGIDATPEELKVIAKDYWDSGIRRIVALRGDIPKGYDKALFYASDL
                     VALLKSVADFDISVAAYPEVHPEAKSAQADLLNLKRKIDAGANHVITQFFFDVESYLR
                     FRDRCVSIGIDCEIVPGILPVTNFKQLQKMAAVTNVKIPTWMGKMYEGLDDDQTTRNL
                     VAASIAMDMVKVLSREGVNDFHFYTLNRSELTYAMCHTLGVRPDLTTA"
     gene            complement(5914..7074)
                     /locus_tag="PXA33_001724"
     CDS             complement(5914..7074)
                     /locus_tag="PXA33_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121038.1"
                     /translation="MKKSILLILSLFSASLFADVQLSPLPLPQIGKVSQFDLSEQGEL
                     VVINQQKQLWRVTQQAQKLADGVSTEIEPKAQYQRIALADQNGHFMLWTADKIYTSNI
                     PLAPKATMTALNFATIAVTKQNGQFKLARIETQGNQATITATAETEVLPDAHPLQIDF
                     DAETPNQGHIAVLAKPDDFTYQHGVLGDDVEAGEIQFLERHTLNPLAEKLTQQGRVFE
                     ANRWATFTENGKTKIVSVISGDGNGASTVVIGVKDGKLSIEAESEPLSTNRWQSPFIF
                     NRKLYAIQMPHLKGNLVEYSLKGNQLAERFITSGLSNHRYGDHETNLVATTETFALIP
                     QMGYQQVVVLDKTGKITELPTALPAGIKQTKADSNKAYLLLKNGEIWVAEEK"
     gene            complement(7134..8150)
                     /gene="galE"
                     /locus_tag="PXA33_001725"
     CDS             complement(7134..8150)
                     /gene="galE"
                     /locus_tag="PXA33_001725"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HDL5121039.1"
                     /translation="MAILVTGGAGYIGSHTLVELLNENREIVVLDNLSNSSEVSLERV
                     KQITGKSVKFYQGDILDRDILRKIFAENQIESVIHFAGLKAVGESVREPLRYYQNNVT
                     GSIVLVEEMLKANVNTIVFSSSATVYGDPQIIPIVESCPVGGTTNPYGTSKYMVERIL
                     EDTVKAFPQLSAVVLRYFNPVGAHESGLIGEDPNGIPNNLMPFISQVAVGKLPQLSVF
                     GGDYNTHDGTGVRDYIHVVDLALGHLKALDKHQNDAGFHVYNLGTGTGYSVLDMVKAF
                     EAANGITIPYKVVDRRPGDIAVCYSAPQKALEQLGWETERGLEQMMKDTWNWQKNNPN
                     GYKG"
     gene            complement(8238..8399)
                     /locus_tag="PXA33_001726"
     CDS             complement(8238..8399)
                     /locus_tag="PXA33_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HDL5121040.1"
                     /translation="MNETLLNNLVCPVSNEKLEWDKENNRLINPKLNIAYPIKNGIPE
                     LLPEAGVAL"
     gene            complement(8377..9378)
                     /gene="lpxK"
                     /locus_tag="PXA33_001727"
     CDS             complement(8377..9378)
                     /gene="lpxK"
                     /locus_tag="PXA33_001727"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HDL5121041.1"
                     /translation="MKIWQTKSLITWLLSPLSLLFWLISQARKRLYSLNILQSYRSPL
                     PMLVVGNISVGGNGKTPVVVWLVEQLQQRGVKVGVISRGYGGKAKTFPQLVTRESSAE
                     MVGDEPVLIVQRTNVPLAISPNRQQSIELLLNQFEPDIIITDDGLQHYALQRDMEWVV
                     VDGVRRFGNGFVLPAGGLRELPSRLNSVQAVICNGGTPQANEHLMTLEPSDAINLVTN
                     QRKPLSEFTQPCVAIAGIGHPPRFFTMLEKLGIPLAKTQGFADHQAFDKAQLAQFADS
                     QTPLLMTEKDAVKCHDFAQPNWWYVPVSATFSEKSTACLLAPILQKLGKEDERNTTQ"
     gene            complement(9380..>9526)
                     /locus_tag="PXA33_001728"
     CDS             complement(9380..>9526)
                     /locus_tag="PXA33_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDL5121042.1"
                     /translation="GNGVEEIQRLQLIISHKDELLAHKEEIIENQKRELALLRDLVET
                     LKNK"
BASE COUNT         2611 a         2072 c         2114 g         2729 t
ORIGIN      
        1 ttagttcagg cagagttaaa atattcaggt agcaatattc caagtgaata ttctaaattt
       61 aacttggaaa atgtgcttat tgattttcct aacaagacaa attctgtagc atatcagaat
      121 ctagttattc acaacgcaaa aacgcgtgca ggaaaattaa aaacacaata tgcaaatgct
      181 cacgatttag gcaatttggt tttagtagag gatatccaaa aaatattttc cgggttatta
      241 gaaataattt atcaagttcg ttgccatttg gtacatggta gcttaagtcc aacacctgaa
      301 aaccacgaag ttgttaaata ttgctatttg attttgtggg attgtttaaa aggattttgt
      361 gattagttat tgtcaataaa gctacaagcg gtcaattctc ccaaaacttt gcaaatttcc
      421 ccgcaaaact gaccgcttgt tctcctctat ccattcacct caaccaactt ttctctctac
      481 ccctactcaa aatcggttaa acaatctgtt gaggtaaatg ttgtgtcgga atgggattta
      541 atcttaatga aactgaaaaa atcttctgcc aagcaaattt ccctaaaatt ggaagttcat
      601 ttccaatttc tcaaaaaatc acccaaaccg gaactacact tccaattttt caatccccca
      661 catttccctt ttctcccaat ccatttcagc tttcacccca aaagccgttg caatattttc
      721 ggcatttaag accgcttgtg tgttgccgct gacgagtaaa ttgccgtttt taagcagaat
      781 gatttcatca caaaaacggt aggcaagggg caaatgatga atcaccacca cgcaggtttg
      841 tgttggagtt agggctttga gctgctgcat aatatcgatt tggtagtagg ggtcgagggc
      901 ggcaatcggt tcatcggcaa gcaagatcgg ggcttgtttc atcaaacaac gggcgagttg
      961 cactcgggtc tgttcaccgc cggagagctg ttggtaagtg ttgtccagca ggtgagaaat
     1021 cgccaattgt tcggcaaccg aacgcacctt ttgacgctct tgttcaggcg gaagcggtgc
     1081 gggcaagccg agtgcaatca catcatacac cgaaagttgc cacgccacgt tgagctgttg
     1141 ggcgaggtag gcaaattgtc ggctcctctc aacggcggaa agggcgtgca gtttgcaatt
     1201 ttccagccaa atttcaccgc ttgtcgggcg tagaatgccg gcaatcgctt tgagcaacga
     1261 ggatttccct gccccgtttg cgcccataat gccgatcaat ttgccttttg gcagatagca
     1321 agagaggtgt tgaataccgt gggcaagggt gagattttca atcttgatca ttgtgccaat
     1381 ctccattgct gggtgaataa aatccaaatc aaaaacggta cgcccaaaat tgccgtgagc
     1441 gtgccgatgt aaattcgggc aaaaaacggc acatattgca ccgccagatc tgcaatcagc
     1501 agtaacaacg ccccgattaa agcggaggta agataaagct gggacggcgg ttttttcagc
     1561 caaatacggg caagatgagg ggctatcaag ccgataaaac cgatggtgcc ggtctgtgga
     1621 atcgttgctc cgaccaacag cgccacgcca atgctaacgg taaaaaaact gcgttgcagg
     1681 ttgatgccca ttgttgctgc cgtctcttcg ccaaaggtga gcaggtcgag ataacgccgt
     1741 tcccgataga ggcagacaat gccgagcaag atcaacggta acgcccaaca tactgcctct
     1801 gcggttgccc agactaatga gccttgcaac cagcggtaga gttccgccaa cgcccacggg
     1861 ctttcggcat tggaaagcag taaagtcacc gctgccccga gcaacacgtt taccgccacg
     1921 ccacttaaaa tcatcattgt tgtgccgtgg cgaaagctaa tcagccacac cagcaaaaat
     1981 gcccccagcg cccctgccac accgccaaac agtagccagc ccatcggggc tgaaaaatag
     2041 tagagcagaa atacgctcac cgccgttgcc cccgaactgc tcccaagcaa accggggctg
     2101 gcaagcggat ttttaaacac gccctgcatc gcattgccgg caagcgcaag gctggcgccc
     2161 gtcagcaccg ccaagccaat acggggcagt cgaatttcac ccagcaccag ccagcgcaga
     2221 tcgcttagca cgccttccgc atttttaagc aaggcaaaat cgctcagttg gtaaaaggct
     2281 gcgttgctaa caatcagcaa caacaagctt ccaagcacaa tgtttaattt cttcattctt
     2341 ctttctctca tcaaacgtgc aaattatacc atagcgccaa agtcaagctg aaaacgctat
     2401 ttttgcgatc atcatcgaaa aactagcgtt tgccgaatgt aacccacgac aggctttgct
     2461 atattccttc aatcagccta agttttgtat ttaaaacatt acagattggg ggtagaatgt
     2521 accatattga acgcactgaa attttcgacc aatggcttga aaatttaaaa gatccacttg
     2581 ctgtggtgac cattgcaaga cgtattgaac gtgccgaaag cggaaatttt ggcgatacca
     2641 aatatatcgc agaaggaatt tccgaaatga ggctaaacgt cagcaaaggt tatcgccttt
     2701 actatgcgca aaaaggtagc attatttata tactactaaa tggcggacat aaaggcacgc
     2761 aacaagctga tattgataaa gctaaaatgc tatgggcaga aattaaacaa catatggcag
     2821 aacaggagaa aaagaatgaa cgaattatat gacgccccaa ttccacaagc tactcgtgaa
     2881 aaatttggca ttcaaccttt cgatgccgct gaatacctcg ccaatgacga gctaatcgcc
     2941 ctttatttgg cggaaacatt gaaagacggc acggacgagg agtttattca agcattgaat
     3001 accgtggcac gagcgaaagg aatgaatgag ttagcgaaaa aagcagggat cgggcgtgaa
     3061 agtttgtatc aaagcctttc cagcagtaag ccccgttttg agactatccg caaaattatt
     3121 agcgcattaa atttggagtt gtcggtcgtt aaggcgtaag tttgtgcatt aattgtatcc
     3181 cctgccaaac cccgtggtcg aaacagtagg tgtatttcat cggtaggaca agcggtggat
     3241 tttggctcat tttttgccat attttttgta atgctgggtg tttgagcagt tcggcttggt
     3301 tgttatagct ttggttatcg gtgaggaaca ataactgttt gggttgcgct aacagcagtt
     3361 tttctagcga gaaattctgt ggcgtgaggt cgctttttaa tggtgacaga ccgagaagat
     3421 ccaacaacgt ttgatattgc ggaaaatgcg gttcgatcat acccgtttcg cttagaatta
     3481 aagtttcagc aagcggttgt tttagcggaa ttttttgcaa acgtagccgt tccaccagct
     3541 tttccgcttt ggcttgattt tgggttagct cgcccagttg taaaatcagt gtgaaaagct
     3601 gttcgggtgt ttgcggactg tcgtttaatg cgactaactt aaagccaagc tgtttcagcc
     3661 gtgcggtgag ttgcggatag aaggtttcat tcagcaaaat ggtgctgtcg gcatagggca
     3721 acagggcggt gagttcaggc tcaacggtgg gcttgtccgt gttcagttta tccaacatca
     3781 tcaacggctt ttgggaatag ggcgacatcg cccgaatttg ctgtggttcg gcaagttcca
     3841 gcaacagacg gtcgctacac aaatttggcg ataaacggta agctcctgaa atacgcccag
     3901 tgatagaatt tttaaactgt gaattatcgg tgtaacggcc actaatagaa aaactgtgat
     3961 cttgttcggt aaataagcga tattctagtg ccgcactttt ttcggataag gttttttcac
     4021 gcaaatattt atcagaggta tagcgtgttt ttgcataatc tgtcagtaaa ctgaccgctt
     4081 gtgtcacttc tccttcacgg tcaaagttta catctaattg ataattcgca ttaagttgtt
     4141 tttcgttgct gtagcgagca tttgaaccga aatgtgttgc tttactattg aattggctga
     4201 tatttgcttg gtgtttcagc agctcttgtt catttcccca ataaccgcct aatttaaaag
     4261 tttgattgcg agtacgggaa tattcaccgc taacgtctga attatagcca tcaatatgaa
     4321 cggtttggga agattgagcg gcaagaactt ctaccccttt gttaccgagg tcataaccta
     4381 aacgtaaaga gccgttatca cgatgaaagc catctcgttc gcttgcacca ccggttttca
     4441 cttctgagcc tgtttctgtg gtatagctga aatggttttt actcagtgcg gaaatgcctt
     4501 tggtacgatg gctatcaccg tgtaaggcat agtagaaacc gttgtgaaat ccggaaagtg
     4561 ttgcgctggc atcacgggta ttatttgagc ctaaaccaag atcgacctcc gcattgaatg
     4621 gtttctcttt gtataaaccg cttttagtgg tgatataaac tacaccaccc atagcactgc
     4681 ttccccaaag ggcggattgc tcgccacgca acacttcgat acgttcgata ttgctaatcg
     4741 gtaagccacc aaaatcgaaa tttccaatat taatcggatt gacttttaca ccgtcaatca
     4801 caaccgcagt atggcgagat tctgcccctc gtaaaaataa gctagtaaga gtaccacgcc
     4861 caccttgttg cccaatcgcc acgcccggca cagtctttaa cacatcactc acataagtcg
     4921 cattgcgtgc ggcaaaatct ttttcggtaa gaaccgtcac gctggaagcg gttttgtctt
     4981 ggttgattgg ggttgcatag gcggaataga ctgttacgga atcaagctga gtcatctcat
     5041 tttgtgcaaa agctgtactt gctagtgcaa cagaaatggc gaatgctgtg ggattctatt
     5101 gatcgcctaa gtcctttaaa accgaaattt gtttcggtaa cttatggagc aaattctggt
     5161 gagcgagatc gtacacacag tattgtgaaa tcaatccaag aaaaaacagg gatcatttca
     5221 gctccgcact taacaggcat tgatgcaaca ccggaagaat taaaggtaat tgccaaagat
     5281 tattgggata gtggtattcg ccgaattgtt gctttgcgtg gcgatatacc gaaaggttat
     5341 gataaagcac tattttacgc ctcagattta gtcgctttat taaaatcagt agcagatttc
     5401 gatatttccg tggctgccta ccctgaagtt caccctgaag caaaatcagc acaggcagat
     5461 ttacttaact tgaaacgtaa aatcgatgcg ggtgcaaacc acgttattac gcaattcttt
     5521 tttgatgtag agagttattt acgttttcgt gatcgctgtg tttctattgg gattgattgt
     5581 gaaattgtgc cgggtattct gccggtgaca aatttcaagc agttgcaaaa aatggcagca
     5641 gtgactaatg ttaagatccc gacttggatg ggtaaaatgt acgaagggtt agatgatgat
     5701 caaaccacca gaaacttagt tgcagcaagt attgcaatgg atatggtgaa agtgctttct
     5761 cgtgaagggg tgaatgattt ccacttctat accttaaatc gtagtgaact cacttatgca
     5821 atgtgccata ctttaggcgt aagacctgat ttaacaactg cataatcgaa cattaaggct
     5881 gtatagctaa atgagctata cctccttata gttttacttc tcttcagcca cccaaatctc
     5941 gccatttttt aataataaat aggctttgtt ggaatcggct ttggtttgtt taattccagc
     6001 cggcagggcg gttggcaatt cagtgatctt gcctgtttta tccaacacaa caacttgttg
     6061 atagcccatt tggggtatca gtgcgaaagt ctctgttgtc gccactaaat tggtttcgtg
     6121 atcaccatag cggtgattgc ttaagccgga ggtaataaaa cgttcagcta attggtttcc
     6181 tttcaggctg tattcaacca aattgccttt tagatgtggc atttggattg catagagctt
     6241 gcggttaaag ataaacggcg attgccaacg gttagtcgac agcggctcac tttcggcttc
     6301 tattgataat tttccgtctt taacgccaat cacaacagtg ctggccccat tgccatcgcc
     6361 agagataaca gaaacgatct tggtttttcc attttctgtg aaagtcgccc agcggttggc
     6421 ttcaaatact cgcccttgtt gggtgagttt ttctgctaag gggtttaggg tatggcgttc
     6481 tagaaattga atttcgcctg cctctacatc atcgcctaat acaccgtgtt gatatgtaaa
     6541 atcatcaggc tttgcaagta cagcaatatg cccttggtta ggtgtttcgg catcaaaatc
     6601 aatttgcaga gggtgagcat caggcaacac ctcggtttct gcggtggcag taatagtggc
     6661 ttggtttcct tgcgtttcaa ttctggctaa cttaaattgt ccgttttgtt tggtcacggc
     6721 aatggtggca aaattaaggg cggtcattgt agctttcggg gcaaggggaa tgttggaggt
     6781 atagatttta tcagccgtcc acagcataaa atgcccgttt tgatccgcta acgcaattcg
     6841 ttggtattga gctttaggtt caatctcggt ggaaactcca tctgccagtt tttgagcttg
     6901 ttgtgtgact ctccataatt gtttttgttg gttaattacc actaattcgc cttgttctga
     6961 aaggtcaaat tggcttactt tgccaatttg gggaagtggg agtggagaaa gctgaacatc
     7021 agcaaacaaa gaagcggaga acaagcttaa gattaacaga atagattttt tcattagcgt
     7081 tacctaaaaa gaagatttaa atgataaagg gagatgttat ctccctttac gctttagcct
     7141 ttataaccat tcgggttatt tttttgccaa ttccaagtgt ctttcatcat ttgttctagc
     7201 ccacgctcgg tttcccaacc cagttgctct aacgcttttt gtggagcgga gtagcaaacg
     7261 gcaatatcgc ccgggcggcg atctaccact ttgtatggaa tagtaatgcc atttgcggct
     7321 tcaaaggctt ttaccatatc tagcaccgaa taacctgttc ctgtgcctaa attgtaaacg
     7381 tggaaaccgg catcattttg gtgcttgtct aaggctttta agtgaccaag tgctaaatct
     7441 accacgtgga tataatcacg cacacctgtg ccatcgtgag tattataatc gccaccaaac
     7501 accgataatt gaggtaattt gcccacagcc acttggctga taaacggcat taagttattt
     7561 gggataccgt ttggatcttc accgattaaa ccgctttcgt gagcccctac cgggttgaag
     7621 taacgcaata ctaccgcact taattgtggg aaggctttaa cggtatcttc aagaatgcgt
     7681 tcaaccatat atttggacgt accgtaaggg ttggttgtgc caccgacagg gcaagattcc
     7741 acaatcggga taatctgcgg atcaccataa acggtcgccg atgaactaaa cacaatggta
     7801 ttcacattgg cttttagcat ctcttctacc aatacaatcg agccggttac attgttttga
     7861 tagtaacgta gcggttctct gacgctttcg cctacagctt ttaaaccggc aaagtgaatt
     7921 accgattcaa tctgattttc cgcaaaaatt ttgcgtaaaa tatcacgatc taaaatatcg
     7981 ccttgataga attttacgct cttgccggtg atctgcttca cacgctcaag tgatacttca
     8041 gaggaattag aaagattatc taacaccacg atttcacgat tttcgtttaa taattcaact
     8101 aatgtatgtg aaccgatgta gcctgcacca cctgtaacta aaattgccat tttatgttcc
     8161 tctataaaat taatacgaac caaggttatt gttgctattt tacccgattt aatcaggctt
     8221 gctaatattt attcagttta taatgccact ccggcttccg gcagtaattc cggaatgccg
     8281 tttttaatcg ggtaagcaat attgagctta gggttaatta atcggttgtt ctctttgtcc
     8341 cattctaatt tttcattaga gacagggcag acgaggttat tgagtagtgt ttcgttcatc
     8401 ttcttttcct agtttttgta gaatcggggc taacaaacaa gcggtcgatt tttctgaaaa
     8461 tgttgcagaa accggtacat accaccaatt tggctgtgca aaatcgtggc attttaccgc
     8521 atctttctcg gtcattaata acggcgtttg gctatcagca aattgagcca gctgagcctt
     8581 atcaaaggct tgatgatcgg caaagccttg agttttggca agcggaatgc ctaatttttc
     8641 taacatcgta aaaaagcgtg gtgggtgacc gataccggca atcgccacac agggttgggt
     8701 aaattcgctt aatggcttgc gttgattggt gactaaatta attgcatcag acggttcaag
     8761 cgtcattaaa tgctcgttag cttgcggtgt gccgccgttg caaataaccg cttgcacgga
     8821 gtttaaacgg ctcggtaatt cacgcaatcc gcccgccggc agcacaaaac cgttaccgaa
     8881 acgccttacg ccatcaacca ccacccattc catatctcgc tgtagggcat agtgttgcaa
     8941 gccatcatcg gtgatgatga tgtccggctc aaactgattt aatagtaatt caatgctttg
     9001 ttggcggttt ggagaaatag ccaaaggcac attggttcgc tgaacaatca gaaccggttc
     9061 atcacccacc atttcagcag aactttctcg ggtaactaat tgcggaaacg ttttcgcttt
     9121 accgccatag cctcgagaaa tcacgcctac ttttacgcca cgctgttgca gttgttccac
     9181 cagccaaacg accactggcg ttttgccgtt gcctccaacc gagatattac cgactaccag
     9241 cataggcaaa ggcgaacggt acgattgcaa aatattcaga gaatataacc gcttgcgagc
     9301 ttgactaatc agccaaaaca gcaacgaaag cggcgaaagc agccaagtga taagggattt
     9361 tgtttgccag attttcatat tatttatttt taagtgtttc aactaaatct cgtaataagg
     9421 ctaattcacg tttttggttt tcgataattt cttctttatg ggctaataat tcatctttgt
     9481 gggagataat aagttgtaaa cgttgaattt cttctactcc atttcc
//
LOCUS       DANLRK010000101         9269 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.101, whole genome shotgun sequence.
ACCESSION   DANLRK010000101 DANLRK010000000
VERSION     DANLRK010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9269
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.101"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            14..229
                     /locus_tag="PXA33_001729"
     CDS             14..229
                     /locus_tag="PXA33_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014990986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HDL5121043.1"
                     /translation="MFPEFRDLITQLKQNNSHFARLFDKHNELDQRIKNMEANIELAT
                     HEEVEALKKEKLHLKDQLYAILKQESA"
     gene            complement(288..1445)
                     /locus_tag="PXA33_001730"
     CDS             complement(288..1445)
                     /locus_tag="PXA33_001730"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HDL5121044.1"
                     /translation="MSLDLADIRTQITNLDRNLLKLLAERHRLAFDVVRSKEITQKPL
                     RDLEREKILLQELVNFAEAENYQLDPQYVTQIFQRIIEDSVLTQQNYLQNKLNAQKEE
                     QVSIAFLGMRGSYSNMASRQFAKKYQGTLAELSCHSFNEVFEKVSLGEADFGVLPLEN
                     TTSGSINEVYDLLQHTDLTLVGELAYPIKHCVLANGTVDLAEVDTIYTHPQPAQQCSQ
                     FLAGLDKVHIKYCESSSHAMQMVARLNKPNIVALGNEDGGKLYGLTNIKTDIANQAHN
                     ITRFIVVAKEAVQVSPQVQTKTLLLMTTTQQAGALVDALMVFKNHHIRMMKLESRPIY
                     GKPWEEMFYVELEANIHSENTQKALAELEQVTSYLKVLGCYPSEIIEPVKL"
     gene            complement(1567..3633)
                     /gene="prlC"
                     /locus_tag="PXA33_001731"
     CDS             complement(1567..3633)
                     /gene="prlC"
                     /locus_tag="PXA33_001731"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HDL5121045.1"
                     /translation="MSNPLLNYTGLPAFSQIKPEHIKPAVEAVIKECRETVEKVAQIE
                     NPTWENFYMPQAMAEDKFSRAWSPVGHLNAVKNSPELREAYQACLPLLSEYSTWAGQH
                     QGLYQGYVKLKNSPEFETYSVAQKKAIENSLQDFELSGISLPAEKQKRYGEISARLSE
                     LNSQFSNNVLDATMGWDIVITDEAQLKGLPESALEAAKLSAESKGKKGYRFTLEIPSY
                     LPVMTYCENKALREEMYRAYVTRASDQGPNAGKWDNSAVIDEILKLRHEKAQLLGFKT
                     YSDYSLATKMAENPQQVLDFLNDLAARSKAQGKNELCDLKKFAKAHFGIEHLDLWDIP
                     FYSEKQKQALYSVNDEELRPYFPENRVLSGLFEVIKRIFGMRVEEQKDVDVWHKDVRF
                     FNIFDKNDRLRGSFYLDLYARENKRGGAWMDDCINQKRLADGTLQKPVAYLTCNFNKP
                     IGDKPALFTHDEVTTLFHEFGHGIHHMLTEIDVGDVAGINGVPWDAVELPSQFLENWC
                     WEEEALAFISGHYETGEPLPKAKLEQLLAAKNFQAAMFVLRQLEFGLFDFRLHTEYDP
                     SKQGQVLALLKEVKDQVAVAKGVEWARTPHSFSHIFAGGYAAGYYSYLWAEVLSADAY
                     SRFEEEGIFNPETGQSFLDNILTRGGSEEPMVLFERFRGRKPTLDALLRHKGIVGNAE
                     PVDCTQ"
     gene            complement(3764..4504)
                     /gene="pflA"
                     /locus_tag="PXA33_001732"
     CDS             complement(3764..4504)
                     /gene="pflA"
                     /locus_tag="PXA33_001732"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HDL5121046.1"
                     /translation="MSVVGRIHSFESCGTVDGPGIRFILFLQGCLMRCKYCHNRDTWD
                     LDGGKEITVEELMKEVTTYKHFMKATGGGVTASGGEAVLQMEFVRDWFRACKAEGINT
                     CLDTNGFVRHYSPVVDEMLEVTDLVLLDLKQLNDEVHQDLVGVSNKRTLDFARYLHKL
                     NKPTWVRYVVVPGYTDDDDSAHRLGQFIQGMDNIEKVELLPYHRLGAHKWETLGYKYE
                     LEGVMSPPKEDLERIQKIIESYGHTVKF"
     gene            complement(4753..5778)
                     /locus_tag="PXA33_001733"
     CDS             complement(4753..5778)
                     /locus_tag="PXA33_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HDL5121047.1"
                     /translation="MSKQDLIRSSVAEYLTFITATGESQVDAVYADENVWLSQKMMAV
                     LYDVDVRTISYHLKKIFSDNELAENSVIQFFWITAQDGKNYHTKHYNLSAIIAVGYKV
                     NSERAVQFRKWATEIVHSFAIKGFAMDDERLKNDGTILGKKYFEEQLARIREIRLSER
                     KFYQKITDIYATAVDYDRTATATKRFFATVQNKLHWAIHGHTAAELIMARANAEKPNM
                     GLTNWKDAPQGKIYPFDVVVAKNYLSDNELAQLQRLVSAYLDMAEDMAERQIPMTMAD
                     WETRLNRFLAATDREILQDAGKVTAEIAKSFALSEFEKYRVKQDLTYESDFDLLVKEV
                     AAKYHAG"
     gene            complement(6013..8337)
                     /gene="pflB"
                     /locus_tag="PXA33_001734"
     CDS             complement(6013..8337)
                     /gene="pflB"
                     /locus_tag="PXA33_001734"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HDL5121048.1"
                     /translation="MTQLTDAQQKAWEGFAAGEWQTEVNVRDFIQKNYTPYEGDESFL
                     AEVTPATSKLWADVMEMIKVENKTHEPYDIDCDTPSTITSHAPGYIDKSLEKIVGLQT
                     DAPLKRAIMPFGGINMVKGSCKVYRRELKPEVEQIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVITGLPDAYGRGRIIGDYRRMALYGADFLMKDKQRQFASLQARLESGEDIQATI
                     QLREEIAEQHRALGKMKEMAASYGFDISGPATNAQEAVQWTYFAYLAAVKSQNGAAMS
                     FGRVSSFLDIYIERDLKAGKITEQEAQELIDHLVMKLRMVRFLRTPEYDQLFSGDPMW
                     ATETLAGMGLDGRTLVTKNSFRILHTLYTMGPSPEPNLTILWSEQLPEAFKRYCAKVS
                     IDTSSVQYENDDLMRPDFQNDDYAIACCVSPMIVGKMMQFFGARANLAKTLLYAINGG
                     VDEKSGDQVGPKTEAITSEYLDYDDVMTRLDSFMDWLATQYVTALNIIHFMHDKYAYE
                     AALMALHDRDVFRTMACGIAGLSVAADSLSAIKYAKVKPIRTDIEIKNKAGEVVGVAK
                     NVATDFEIEGEYPQFGNNDNRVDDIAVDLVERFMKKIQKLKTYRNATPTQSVLTITSN
                     VVYGKKTGNTPDGRRSGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTF
                     SIVPNALGKDYEAQKRNLAGLMDGYFHHEATVEGGQHLNVNVLNRDTLLDAVEHPEKY
                     PQLTIRVSGYAVRFNSLTKEQQMDVITRTFTESM"
     gene            complement(8427..9251)
                     /gene="focA"
                     /locus_tag="PXA33_001735"
     CDS             complement(8427..9251)
                     /gene="focA"
                     /locus_tag="PXA33_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HDL5121049.1"
                     /translation="MFSPAEMAKIAEDGAVYKATKNQLYSFLSAITAGGFIAIAFVFY
                     TTTQVGASQMPWGMAKLIGGLVFSLGVIMCVVFGAELFTSSTLTIVARASQRINWLQM
                     FKNWVVVYFGNFVGAISIVLLIWFSGQIMAAKGQWGLVILNTAQHKIHHQWFEAFCLG
                     IFCNIMVCIAVWMSYAGKSLTDKAFIMMLPIAMFVASGFEHCVANMFMIPMGIAISHF
                     ASPEFWTAINVDPTQFADLDVSHFVFKNLIPATLGNIVGGVFFIGLMQWFLYIRKH"
BASE COUNT         2594 a         2099 c         1832 g         2744 t
ORIGIN      
        1 ttaggagtta aatatgtttc cagaatttcg tgatctcata acccaattaa agcaaaacaa
       61 ttcacatttt gctcgcttat ttgataaaca taatgagcta gaccagcgta ttaagaatat
      121 ggaggcaaat attgaactgg caacacatga agaagttgag gcactaaaaa aagaaaaatt
      181 gcatttaaaa gaccaacttt atgcaattct aaaacaagaa tcggcttaac ctaaagtaaa
      241 agctcagaaa atattctgag ctttaatttc ggtcatatta gacaatatta cagttttacc
      301 ggctcaataa tttcgcttgg gtagcaaccc aacactttga ggtagctggt aacttgctcc
      361 aattccgcca atgctttttg ggtgttctcg gaatgaatat ttgcctccag ctccacataa
      421 aacatctctt cccaaggttt gccgtaaatc gggcgggatt ccagtttcat catacgaata
      481 tgatgatttt tgaataccat taacgcatcg accaatgcac ccgcctgttg cgtggtggtc
      541 attaacagta acgttttggt ttgtacctgt ggcgaaacct gcacagcctc tttggcaaca
      601 acaataaaac gggtgatatt gtgagcttga ttagcaatat cggttttgat attcgtcaag
      661 ccatacaatt ttccgccatc ttcattgccc aaggcaacaa tattcggctt attcaagcga
      721 gccaccattt gcattgcgtg cgagctgctt tcgcaatatt tgatatgtac tttatctagc
      781 ccggctaaaa attggctaca ttgctgggca ggctgagggt gggtgtaaat ggtatccacc
      841 tccgccaaat caaccgtgcc gtttgccaat acacaatgtt tgattgggta cgccaattca
      901 cccactaagg ttaaatcggt atgctgcaat aaatcataca cttcattgat cgaacctgaa
      961 gtcgtgtttt caagcggcag tacgccaaaa tcggcttcac caaggctgac tttctcaaat
     1021 acttcattaa aagaatggca gcttaattcc gccaatgtgc cttgatattt tttggcaaac
     1081 tgtcgagatg ccatattaga ataagagcct cgcattccca aaaacgcaat cgaaacctgc
     1141 tcttcttttt gtgcattcaa tttattttgc agataatttt gctgagtcag cactgaatct
     1201 tcaataatac gttggaaaat ttgggtcaca tattgcggat caagctggta attctccgct
     1261 tcggcaaaat ttaccagttc ttgaagcaaa atcttttccc gttctaaatc tctcaagggt
     1321 ttttgtgtga tttctttgct ccgcaccaca tcaaaggcta agcggtggcg ttctgccagt
     1381 agctttaata aattgcggtc taagttggtt atttgggttc gaatatcggc taaatctaaa
     1441 gacattattt cactctctgt caggctaaat gctgaaaatt ataacgagtt gcaaaagaaa
     1501 gaaaagacaa gcggtcaaat ttacaaaaaa atttgcaaat ctaaccgctt gtaaaacgtg
     1561 tttactttat tgagtgcaat caacaggctc ggcattacct acaatgcctt tgtgtctgag
     1621 caaggcatca agggttggct tacgcccacg gaagcgttcg aacagcacca ttggctcttc
     1681 tgaaccgcct cgggttaaga tattatctaa gaaagactgc cccgtttcag ggttgaaaat
     1741 cccctcttcc tcaaagcgtg aataggcatc tgctgaaagc acttccgccc ataaataact
     1801 gtagtagccc gccgcatagc cgcctgcaaa aatatgcgag aaactgtgtg gcgttctcgc
     1861 ccactctacg cctttggcaa cagcgacttg gtctttcacc tctttaagta aggctaacac
     1921 ttgtccttgt ttgctcgggt catattcggt gtgtaggcgg aaatcaaaca agccaaattc
     1981 taattggcga agcacaaaca ttgccgcttg gaagtttttc gccgccaata attgctctaa
     2041 tttcgctttt ggtaacggct cgccggtttc ataatgccct gaaataaagg ctaatgcctc
     2101 ttcttcccag caccagttct ctaagaattg gcttggcagc tccactgcgt cccacggcac
     2161 accgttaatg ccggcgacat cgcccacgtc aatttcagtt agcatatggt gaatgccgtg
     2221 tccaaactcg tggaacaaag ttgtcacttc atcgtgggta aacaatgccg gtttatcgcc
     2281 aatcggttta ttgaagttac aggttaaata tgccaccggt ttttgtaatg tgccgtcagc
     2341 caagcgtttt tggttgatgc aatcatccat ccacgcccca ccacgtttgt tttcacgagc
     2401 gtataaatct aagtagaacg agccacgcaa gcggtcattt ttatcaaaaa tattgaaaaa
     2461 acgtacatct ttgtgccata catccacatc tttttgttct tccacacgca taccgaaaat
     2521 gcgtttaatt acttcaaata agcctgataa tacgcgattt tccgggaaat acgggcgaag
     2581 ttcttcatca ttgaccgaat acaatgcctg tttttgtttt tcgctgtaga acggaatatc
     2641 ccataaatcc aaatgttcaa tgccgaaatg ggctttagca aattttttca aatcgcataa
     2701 ttcatttttg ccttgagctt tcgagcgagc ggctaaatcg ttcaagaaat ctaatacctg
     2761 ttgtggattt tctgccattt tggtggctaa tgaataatca gaataagtct taaagccgag
     2821 aagttgggct ttttcgtgac gcagttttaa gatctcatca atcaccgcac tgttatccca
     2881 tttgcctgca ttcgggcctt gatcagaagc acgagtcaca taagcacggt acatttcctc
     2941 acgcagtgct ttgttttcac aatacgtcat caccggcaaa tagcttggga tttcaagagt
     3001 gaagcgatag cctttcttac ctttgctttc tgcggataat tttgccgctt ctaatgccga
     3061 ttccggcaag cctttaagct gtgcttcatc ggtaattaca atatcccaac ccattgttgc
     3121 atcaagtacg ttgttgctaa attgcgagtt gagttccgac aagcgtgcag aaatttcccc
     3181 gtagcgtttt tgtttctctg ccggcagtga aatgcctgat aattcaaaat cttgtaagct
     3241 gttttcaatc gcctttttct gtgcaaccga ataagtttca aactcagggc tgttttttaa
     3301 cttcacatag ccttgatata acccttgatg ttgccccgcc caagtgctgt attccgataa
     3361 taacggcaag cacgcttggt aagcctcacg caactctggg gaatttttca ccgcattcaa
     3421 atgccctacc ggtgaccacg cacgggagaa tttatcctct gccattgctt gtggcatata
     3481 gaaattttcc cacgtcgggt tttcaatttg tgcgactttt tcaacagttt cacggcattc
     3541 tttgatcacc gcttctaccg ccggtttgat atgttcaggt ttgatttgtg agaacgcagg
     3601 caagcctgtg taatttaaaa gagggttaga cattattttt tccttttatg gtcaaaatga
     3661 aaattcgtat caagataata cacaaagtga gagagaaaag aaaacaagcg gtcatatttc
     3721 aataattttt tgcaaaaaaa tcacaaaata tgaccgcttg tctttagaat ttgactgtat
     3781 gcccgtaact ttcaatgatt ttttggatac gctctaaatc ctctttaggc ggtgacatta
     3841 ccccttccag ctcatattta tagcctaagg tttcccattt gtgagcccct aaacgatgat
     3901 aaggcagaag ttccactttc tcaatgttat ccattccttg aataaactgc cctaaacgat
     3961 gggcagaatc atcgtcatca gtataacccg gcacgaccac atagcgaacc caagtcggtt
     4021 tatttaattt gtgcagataa cgggcgaaat caagggtacg cttattcgac accccgacta
     4081 aatcttggtg aacttcatca ttgagctgtt ttaaatccag caagaccaaa tcggtcactt
     4141 caagcatttc atccaccacc gggctataat ggcgaacaaa accattggta tctaagcagg
     4201 tattaatgcc ttcagcctta caggcacgaa accaatcccg cacaaactcc atttgcaaca
     4261 ccgcttcccc acccgaagct gttacaccac cgccggttgc tttcataaaa tgcttataag
     4321 tcgtgacttc tttcatcagc tcttcaaccg taatttcctt accaccatca agatcccaag
     4381 tatcacgatt atggcaatat ttacaccgca tcaaacagcc ttgcaagaac agaataaaac
     4441 gaatccccgg tccatctacc gtaccgcagg attcaaaaga atggatacgt ccaacaacag
     4501 acatttgtca cctcaaccct caaaaaattt tgagaaattg tagcaaattt ttatgttgtt
     4561 cgcagaaata aatcacaccg gtatatttta agaaaggaga aagtcatact attttttgcc
     4621 tatttcagga ataaactttt attaatctat tgactccatc aacatatcaa ccaaatatgt
     4681 ttaaattttt gcatttttta aagctatttt gaccgcttac aagcagtcgg tttttctgaa
     4741 aactttgcaa acctagccgg cgtggtattt ggcggcgacc tcttttacca gtaagtcgaa
     4801 atcgctttcg taggtgagat cctgcttgac tcggtatttt tcaaattcgc tgagggcgaa
     4861 agatttagca atttcagcgg taactttgcc ggcatcttgt aagatttctc ggtcggtggc
     4921 ggcaagaaag cggttaaggc gggtttccca gtctgccatt gtcatcggaa tttggcgttc
     4981 tgccatatct tccgccatat ccaaataggc agagaccagc cgttgtagtt gtgctaattc
     5041 attatccgat aaataatttt tggcaaccac cacatcaaag gggtaaattt tgccttgcgg
     5101 agcgtctttc caattcgtta gccccatatt cggcttttca gcgttggctc gtgccataat
     5161 cagctcggcg gcggtatgcc cgtgaattgc ccaatggagc ttgttctgca cggtggcaaa
     5221 aaagcgtttg gtggcggttg cggtgcggtc gtagtccacg gcggtagcgt aaatgtcggt
     5281 gattttttgg tagaacttgc gttcggataa ccgaatttcc cgaatacgag caagctgttc
     5341 ttcaaagtat tttttgccca aaatcgtgcc gtcatttttc agccgttcat cgtccatcgc
     5401 aaagccttta atcgcaaagc tatgcacgat ttcggttgcc catttgcgaa actgcaccgc
     5461 tcgctcggaa ttgactttat aacccaccgc aataattgcc gacagattat aatgcttggt
     5521 gtggtagttt ttgccgtctt gggcagttat ccaaaaaaat tggataactg aattttcagc
     5581 taactcattg tcactaaaga tttttttgag atgatagcta atggttcgta catcaacatc
     5641 atacaacacc gccatcattt tttggctaag ccatacattt tcatcggcgt aaacggcatc
     5701 aacttggctt tcgccggtag ctgtaataaa agtgaggtat tccgccacag aggaacgaat
     5761 taaatcttgc ttagacatct ctttctccca tatagaacac tgtaattata aacaatatta
     5821 cgaaagctgt gtaaatgaat agccaatttt tcgagcgaga tcacaaaaaa accgcttgcg
     5881 gcataaatgc tacaagcggt tgattattac tcacaaaatt gtttccaatt ttgttcgctc
     5941 ctacggagcc gtcggcaaag ccaacgttca aaacgcaatg cgttttgtgg ctaaaagttt
     6001 tgcaaattgt aattacatac tttcagtgaa ggtgcgggta attacgtcca tttgttgctc
     6061 tttggttaaa gagttgaaac gtaccgcata gcctgaaaca cgaatcgtta attgcggata
     6121 tttttcaggg tgttctaccg catctaataa tgtatcacgg tttaacacgt tcacatttaa
     6181 gtgctgaccg ccttctacag ttgcttcgtg gtggaagtag ccgtccatta agcctgcaag
     6241 gttacgtttt tgggcttcgt aatctttacc taacgcattt ggtacgatag agaaagtata
     6301 agaaatacca tctttcgcat aggcaaacgg taatttcgcc acagaagtta atgaagctac
     6361 cgcacctttc tggtcacgac cgtgcattgg gttcgcaccc ggtccgaatg gagcacctga
     6421 gcgacgaccg tccggtgtat taccggtttt cttaccatat accacgttag aagtgatagt
     6481 aagcacagac tgagtcggcg ttgcattgcg gtatgttttg agtttttgaa ttttcttcat
     6541 aaaacgttca actaaatcaa ctgcaatgtc gtccacacgg ttatcgttgt taccgaattg
     6601 cggatattca ccctcaattt caaagtcggt tgctacgttt ttcgctacac caaccacttc
     6661 gccagctttg tttttgattt caatatcggt acggattggt tttactttcg catatttaat
     6721 ggctgaaagt gagtctgccg ctactgaaag ccctgcgata ccacacgcca ttgtgcggaa
     6781 cacatcacgg tcgtgaagag ccattaatgc cgcttcgtat gcatatttat cgtgcataaa
     6841 gtggataatg ttcagtgctg tcacatattg ggttgcaagc caatccataa agctgtctaa
     6901 gcgggtcatt acatcatcgt aatctaagta ttcacttgta attgcttcag ttttaggccc
     6961 tacttggtcg cctgatttct catcaacacc gccattgatt gcgtataaca aggttttcgc
     7021 taagtttgca cgagcgccga agaactgcat cattttaccc acgatcattg gcgatacgca
     7081 gcacgcaatc gcatagtcat cgttttggaa atccggacgc attaagtcat cgttttcata
     7141 ttgaactgat gaagtatcaa tagacacttt cgcacagtag cgtttaaatg cttccggtaa
     7201 ttgttcagac caaagaatag ttaagtttgg ctccggtgac ggccccattg tgtaaagggt
     7261 gtgtaaaata cggaagctgt ttttggttac taaggtacga ccgtctaaac ccataccggc
     7321 taaggtttcg gttgcccaca ttgggtcgcc tgagaataat tgatcgtatt ccggagtacg
     7381 caagaaacgc accatacgca atttcattac taaatggtcg attaactctt gggcttcttg
     7441 ctcggtaatt ttgccggctt ttaagtcacg ttcgatataa atatctaaga atgaagacac
     7501 acgaccgaaa gacatcgccg caccgttttg cgatttaacc gctgcaaggt aagcgaaata
     7561 tgtccattgc accgcttctt gtgcattggt tgcaggacct gaaatatcaa aaccgtagct
     7621 tgcagccatc tctttcattt tgcctaaagc acggtgttgc tcggcaattt cttcacgcag
     7681 ttgaatcgtg gcttgaatat cttcgccgga ttctaaacga gcttgtaatg aggcaaactg
     7741 gcgttgtttg tctttcatta agaagtctgc accataaagt gccatacggc ggtagtcacc
     7801 gataatacga ccacgaccat aagcatccgg caaaccggta attacacctg atttacggca
     7861 acgtaaaata tccggagtgt aaacatcgaa taccccttgg ttatgggttt tacggtattc
     7921 ggtaaagatt tgttctactt caggtttaag ctcacggcga taaactttgc acgaaccttt
     7981 caccatattg ataccaccaa acggcataat cgcacgtttt aatggagcat ctgtttgcag
     8041 accaacgatt ttttctaacg atttatcaat ataacccggt gcgtgtgagg taatggttga
     8101 tggcgtgtcg cagtcaatat catacggttc gtgtgttttg ttttctactt tgatcatttc
     8161 catcacatct gcccacaatt tgctggttgc aggtgttact tcagctagga aagactcatc
     8221 accctcataa ggtgtgtagt ttttttggat aaaatcacgt acatttactt cagtttgcca
     8281 ctcgcctgcg gcaaaacctt cccacgcttt ttgttgagcg tctgttagtt gagtcatttt
     8341 aagtcctctg tgttagtaag ttaaaacggg aaagcggtga ttttttgcaa aaaatttgca
     8401 aaatttgacc gcttgaatct gaaatcttaa tgctttctaa tgtataagaa ccactgcatt
     8461 aaaccaatga aaaacacgcc accgacaata ttacccagcg ttgccggaat taagttctta
     8521 aacacaaaat gagatacatc taaatcggca aattgcgttg ggtcaacatt aattgctgtc
     8581 caaaattcag ggctggcaaa gtgcgaaatc gcaatcccca tcggaatcat aaacatattt
     8641 gccacacagt gttcaaagcc ggaagccaca aacattgcaa tcggtaacat cataataaag
     8701 gctttatcgg ttaggctttt acctgcataa gacatccaca ccgcaatgca caccataatg
     8761 ttacagaaaa tgcctaaaca gaaagcctca aaccattggt ggtgaatttt gtgctgtgcg
     8821 gtatttaaaa taaccaaacc ccattgccct ttggctgcca taatctgacc actaaaccaa
     8881 atcaacaata caatggaaat cgccccaaca aagttaccga aatacaccac tacccagttt
     8941 ttaaacatct gtaaccagtt aattctttgg ctggctctgg ctacaatcgt aagtgttgat
     9001 gaggtaaaaa gttctgcccc aaacaccaca cacataatca cgccgagtga aaataccaaa
     9061 ccaccaatca gtttagccat accccaaggc atttggctcg cacccacttg ggttgtggta
     9121 taaaatacaa atgctattgc aataaaaccg cccgcagtaa tggcagagag aaaggaatat
     9181 aattgatttt ttgttgcttt ataaaccgca ccatcttctg cgatttttgc catttcagca
     9241 ggagaaaaca tcagacaacc ttaacaatg
//
LOCUS       DANLRK010000102         9089 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.102, whole genome shotgun sequence.
ACCESSION   DANLRK010000102 DANLRK010000000
VERSION     DANLRK010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9089
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.102"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(120..195)
                     /locus_tag="PXA33_001736"
     tRNA            complement(120..195)
                     /locus_tag="PXA33_001736"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(160..162),aa:Asn,seq:gtt)
     gene            complement(201..276)
                     /locus_tag="PXA33_001737"
     tRNA            complement(201..276)
                     /locus_tag="PXA33_001737"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(241..243),aa:Phe,seq:gaa)
     gene            complement(408..1499)
                     /gene="mltC"
                     /locus_tag="PXA33_001738"
     CDS             complement(408..1499)
                     /gene="mltC"
                     /locus_tag="PXA33_001738"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HDL5121050.1"
                     /translation="MKKYAKYLPMLAIIPFLIACGSNKSTKKPKSTRVDYSKDTNGLD
                     ILAGQFSHNIDDIWGSNELLVASKKDYVKYTDKFYTRSHISFEEGVITIETLGNQNHL
                     RNSIIHTLLMGRDPKGIDLFASGDAPISSNPFLNGQVEDQFGRNITNVAIANDFATYL
                     LQNKVQTRRLKNGQTVSFVTIKMVAGHIEVRARQYLPLVRKMAKQYGIEPSLILGIME
                     VESAFNPYAISYANAIGLMQVVPRTAGRDIFARKGMGGQPSREYLYNPSNNIDAGTLY
                     LTILRDEYLDGIEDPVAKRYAMISAYNSGAGAVLRVFDSDRYSAIDRINDLSSDAVYR
                     ILTTAHPSSQARNYLRKVTTAREKYRNIR"
     gene            complement(1516..1785)
                     /locus_tag="PXA33_001739"
     CDS             complement(1516..1785)
                     /locus_tag="PXA33_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HDL5121051.1"
                     /translation="MARTVFCEYLKQEAEGLDFQLYPGELGKRIFDSISKQAWSEWIK
                     KQTMIVNEKKLSMMNPEHRKLLEQEMVNFLFEGKEIIIEGYKPVE"
     gene            complement(1789..2934)
                     /gene="mutY"
                     /locus_tag="PXA33_001740"
     CDS             complement(1789..2934)
                     /gene="mutY"
                     /locus_tag="PXA33_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005609167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HDL5121052.1"
                     /translation="MNSNSPFPLAKAKASDPFAKAVLAWFEQYGRKHLPWQQNKTLYQ
                     VWLSEVMLQQTQVATVIPYFARFMERFPTVIDLANASIDEVLHLWTGLGYYARARNLH
                     KAAIQIRDEFGGEFPTRFDDVLALTGVGRSTAGAILSSVLDAPHPILDGNVKRVLSRY
                     FAVEGWAGEKAVENRLWQLSESVTPDTQVADFNQAMMDLGAMICTRTKPKCLLCPLQE
                     NCKTNAMQAWDSFPAKKPKKTLPERQTYFLVLKSGSKVLLQKREAKGIWGGLFVFPQF
                     ESLEALKRSDFMQNLQISQQLTAFRHTFSHFHLDITPVLVETDLKKTSENSPLVVEEK
                     AGNYLSKVSSNADYWYDLHQHNEVGLATPVKRILDELAFSVKNEIKD"
     gene            3018..3410
                     /locus_tag="PXA33_001741"
     CDS             3018..3410
                     /locus_tag="PXA33_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL5121053.1"
                     /translation="MKSPILGFHHIALIVSDYEKSKHFYTRILGAEIIAETYREQRAS
                     YKLDLRFKDGSQIELFSFPETPPRFTSPETCGLRHLAFRVENIEYAIAFLEQHQLPHE
                     GIRIDEITGKRFTFFRDPDDLPLEFYEV"
     gene            3411..4280
                     /locus_tag="PXA33_001742"
     CDS             3411..4280
                     /locus_tag="PXA33_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDL5121054.1"
                     /translation="MLYLLIATLIWASSFIVGKVAISAFDPVQIMQIRLTLAAFIALP
                     FFIPAYKKIQKNQHIKVWLISFLTFPLYVILQFSGLKLTSAASAVALLGLNPLLTVLV
                     GYFCFKKQATRLDFILSLFACLGILIMASGSDDSGSINVLGLLLVTLGSLSFIVGMYL
                     SKEILQSVKVIDLTKIMLVQGAITALPITLLFAENWQLPTSLSAWASVAYLGIICTSL
                     AILLWNKGISQSSPILAGILLALEPVFGLLMALVFLSEQLSLVTWVGIIIVICTALLS
                     VLLPMMKRYKRLI"
     gene            4296..5009
                     /gene="sfsA"
                     /locus_tag="PXA33_001743"
     CDS             4296..5009
                     /gene="sfsA"
                     /locus_tag="PXA33_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HDL5121055.1"
                     /translation="MKFPPLSHGILLRRYKRFLADIQLPNGEQITLHCPNTGAMTGCA
                     NPGDTVWFSTSHNPKRKYAHTWELTQTQAGDFICVNTLRANQLVQEALENQWISELAD
                     YQHILPEQKYGSENSRIDFLLKSDLLPDCFVEVKSTTLLTENGVGMFPDAKTERGQKH
                     LRELTKIAEQGLQAVIFFAILHTGIERFEVAKQIDPKYAELFAQAIAQKVQVLSYKMA
                     ISLENGQPIAATLTKKCEF"
     gene            5070..5591
                     /gene="hslV"
                     /locus_tag="PXA33_001744"
     CDS             5070..5591
                     /gene="hslV"
                     /locus_tag="PXA33_001744"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HDL5121056.1"
                     /translation="MTTIVCVRKDGKVAIGGDGQATLGNCIEKGTVRKVRRLYKDKVV
                     TGFAGSTADAFILRDLFEKKLELHQGHLVKSAVELAKEWRTERALRRLEAMMIVANDS
                     EFLLVSGSGDVIEPEQDVLAIGSGGNYAKAAALALLRTENNLSAKEIVAEALKIAGDI
                     DIYSNYNHVIEEV"
     gene            5848..7173
                     /gene="hslU"
                     /locus_tag="PXA33_001745"
     CDS             5848..7173
                     /gene="hslU"
                     /locus_tag="PXA33_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HDL5121057.1"
                     /translation="MMSMTPREIVSELDAHIIGQKDAKRAVAIALRNRWRRMQLPEDL
                     RQEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LADVSMKLVRQQAVEKNKMRAQDAAEDRILDVLLPPAKDQWGNVQESDNNSTRQTFRK
                     KLREGQLDDKEIEIDVAAQVSVEIMTPPGMEEMTSQLQSLFEGMSPSKTKKRKMKIKD
                     ALKVMLDEEAAKLVNPEELKQQAIEAVEQHGIVFIDEIDKICKKGEHSGGDVSREGVQ
                     RDLLPIIEGSTVNTKHGMVKTDHILFICSGAFQVARPSDLLPELQGRLPIRVELKSLT
                     KEDFERILTEPNASLTLQYRELMKTEGVEIEFTKDGISRIAESAFRVNEKTENIGARR
                     LHTVLERLMDGISFDASERRGEKIIIDENYVSEALNDVVENEDLSRFIL"
     gene            7249..8127
                     /locus_tag="PXA33_001746"
     CDS             7249..8127
                     /locus_tag="PXA33_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF535 domain-containing protein"
                     /protein_id="HDL5121058.1"
                     /translation="MKNLYKWPAAQSLYPNQGKKSYAGKRFRYRLRSWLHHSKIKQFE
                     QFVTQNPQLIPLLNARPNYSYPVAHRFLDKRFSAKQRLQKITDNLLFLPQKLAHLPPL
                     WEQAVNFGEIIADFELWLNINEHQPMEGFWALELRHRPTNQLVYLLTFGKLDEALLIA
                     VIQGPNFEGSKELVKQLTKSCHGLRPAYLMVETMKALTAVLGYQSLLGIPQKYQNKSR
                     WVQSKRYVVDYDAIFAESGGKPGNYWQLPLELESKKIDEIPSKKRSMYRKRYEMLANL
                     KQTMELMLKTYTGLNG"
     gene            complement(8197..8913)
                     /locus_tag="PXA33_001747"
     CDS             complement(8197..8913)
                     /locus_tag="PXA33_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL5121059.1"
                     /translation="MKKTLLTLLFGCVVTAQAQDIKFVMEPSYPPFEMTEEKGEIIGF
                     DVDIANAICKEMNANCTFHSQPFDSLIQSLKQKQFDAAISGMGITEPRKKQVLFSEPY
                     FPSSAAFIAKKDTDFAKVKTIGVQNGTTYQHYLAKEKKEYNVKSYASYQNAILDVQNG
                     RIDAIFGDVPVLAEMARKHEGLDFVGEKINNPNYFGDGLGIATHLKNQVLVDQFNAAL
                     KTIKENGEYQKIYDKWMGGK"
BASE COUNT         2699 a         1926 c         1832 g         2632 t
ORIGIN      
        1 aattctcaaa aatttagcgt ttttgaacgc ataatactaa aaattgagca atttcttagt
       61 agttagccgg aaagctcaaa aagaaaaccc tgatgattac tcatcagggc ttcagaatat
      121 ggctcctcct gcgggactcg aacctgcgac atatggatta acagtccacc gttctaccga
      181 ctgaactaag gaggaattga tggtgcctcg aggcggaatc gaaccaccga cacggggatt
      241 ttcaatcccc tgctctaccg actgagctat cgaggcaacg tcgttgcgtg gtgcattatg
      301 ctgatttcag cttgcaacgt caaacgtttt tttgcaaatt taaaaaaacc gctgctcaaa
      361 tgtgcttttt ttatactttt cggctaaaaa acagcataat tcacacatta acgaatattg
      421 cgatatttct ctctggcggt ggttactttt cttaaataat ttctagcttg agaagatgga
      481 tgagcagtgg ttaaaattcg ataaaccgca tcagacgaaa gatcgttaat tctatcaatc
      541 gcagagtatc ggtctgaatc aaacactcgt aataccgcac ctgctccgct attgtatgca
      601 gaaatcattg catagcgttt agccacagga tcttcaattc catctaagta ttcatcacgc
      661 aaaatagtca aataaagcgt acctgcatca atgttatttg atggattata aagatattca
      721 cgacttggct gccctcccat acctttacgg gcaaaaatat cacgcccggc ggtacgaggt
      781 acaacctgca ttaagccaat cgcattggca tagctgatgg cataagggtt gaatgctgat
      841 tcgacttcca taatccctaa aatcaaactt ggctcaatac catactgctt cgccatttta
      901 cgcaccagcg gtaaatattg tctggcacgt acttcaatgt gtcctgccac cattttaatc
      961 gttacaaagc tcacagtttg accatttttc aaacggcgtg tttgcacctt attttgcaac
     1021 aaataagtcg caaaatcatt ggcgatggca acattggtaa tatttcgccc gaattgatct
     1081 tcaacctgtc cgtttaggaa agggttagag ctaatcggtg catcgcctga ggcaaataaa
     1141 tcaatccctt tcgggtctct gcccattaat aaagtatgaa taatcgaatt gcgtagatga
     1201 ttctgattcc ctaaagtctc aatcgtaatc accccttctt caaacgaaat atggctacgg
     1261 gtgtaaaact tatccgtata tttcacataa tctttcttac tggcaactaa cagctcgtta
     1321 cttccccaaa tatcatcaat attgtgagaa aactgccctg ccaaaatatc caaaccattg
     1381 gtatctttag aataatctac tcgggtagat tttggcttct tagtcgattt attgctccca
     1441 caagcaataa gaaacggaat aattgccaac atcggcaaat atttagcata ttttttcatt
     1501 gtgatttaac ctttattatt cgaccggctt atagccttcg atgatgatct ctttaccttc
     1561 aaataaaaaa ttgaccatct cttgttcgag cagtttacgg tgttcggggt tcatcatact
     1621 gagttttttc tcattgacga tcattgtctg ttttttaatc cactcagacc acgcttgctt
     1681 gctgattgag tcaaaaatac gcttacccag ctcccccgga taaagttgaa aatctaaacc
     1741 ttctgcctct tgttttaaat attcgcaaaa tacggtacga gccataattt aatccttaat
     1801 ctcatttttc acactgaatg ccagctcatc taaaatacgt ttaaccggcg ttgctaaacc
     1861 cacttcgtta tgttggtgca aatcatacca ataatccgca tttgaagata ccttagaaag
     1921 ataatttcct gctttttctt cgacaacaag cggtgaattt tcacttgttt ttttcaaatc
     1981 ggtttctact aaaaccgggg taatatccaa atgaaaatgg ctaaaagtat gcctaaatgc
     2041 agttaattgc tgtgaaattt gcaaattttg cataaaatca gaccgcttga gagcctctaa
     2101 gctctcaaat tgcggaaaca caaataagcc cccccaaatg ccttttgctt cccgtttttg
     2161 taacagcact ttcgagccgc ttttcagcac taaaaaatag gtttgacgct ccggcaaggt
     2221 ttttttcggt ttcttggcag gaaagctatc ccacgcttgc attgcattcg ttttacaatt
     2281 ttcttgtagc gggcagagca agcacttagg ctttgtacgg gtgcaaatca tcgcccctaa
     2341 atccatcatc gcttggttaa agtctgccac ttgggtatca ggcgtcacac tttcgcttaa
     2401 ttgccaaagg cgattttcca ccgctttttc acccgcccag ccttctactg caaaatagcg
     2461 gcttaacacc cgtttgacat tgccatctaa aatcgggtgt ggggcatcta acacagaaga
     2521 caaaatcgcc cctgcagtac ttctgcccac tccggttaat gccaacacat catcaaatcg
     2581 ggtaggaaat tcaccgccaa actcatctcg aatttgaatt gccgctttat gcaaatttct
     2641 tgcccgagcg taataaccca gccctgtcca caaatgcaat acttcatcaa tcgaggcatt
     2701 agctaagtca attacggtgg gaaatcgttc cataaaacgt gcaaaataag gaataacggt
     2761 cgctacttga gtttgttgca acattacctc cgacaaccaa acctgataca gcgttttatt
     2821 ttgctgccaa ggtaaatgtt tccgcccata ttgctcaaac caagctaaaa cagctttggc
     2881 aaacggatca gaagcttttg ctttcgctaa aggaaatggg gaattgctgt tcattaaaaa
     2941 tcttgaaatc cgaaaaataa attatcatac tgcaagcggt cagttttacc aaaaatttta
     3001 cacattaagg aaaaaagatg aaatcgccca ttttaggttt ccaccacatt gcattaattg
     3061 tttccgatta cgaaaaatcc aaacatttct acacccgcat tttaggggca gagatcatcg
     3121 cagaaaccta ccgagagcaa cgtgccagct ataaattaga tttacgcttt aaagatggct
     3181 cacaaattga attattcagt tttcctgaaa cgccgcctcg tttcacctcg cccgaaacct
     3241 gcgggcttcg ccatttggca tttcgagtgg aaaatattga atatgccatc gcatttttag
     3301 aacaacatca attaccgcat gaagggatcc gaattgatga gatcaccggc aaacgcttca
     3361 cgtttttccg agatccggac gatctgccgt tagagtttta cgaggtctaa atgttatatt
     3421 tactgattgc aaccctgatt tgggcgagtt cattcattgt aggtaaagtg gcgatttcag
     3481 cctttgaccc tgtccaaatt atgcaaattc ggctgacgtt agcggcgttt atcgctctgc
     3541 cgttttttat tcctgcgtat aaaaaaatcc agaaaaatca gcacattaaa gtctggctga
     3601 ttagttttct cacttttcct ctgtacgtga tcttacaatt ttccggcttg aaattaactt
     3661 ccgcagcaag tgccgtggca ctactcgggc taaacccgct tttaactgtg ttagtcggct
     3721 atttttgttt caaaaaacag gcaaccaggc tcgattttat cctaagttta tttgcctgct
     3781 tggggatttt aattatggca tcgggtagcg atgatagcgg ttcaatcaat gtattaggat
     3841 tattgcttgt aaccttaggc agtttaagtt ttattgtggg aatgtacctc agcaaagaga
     3901 ttctgcaatc agtaaaagtg atcgatttaa ccaagatcat gctggtgcaa ggggcaatta
     3961 ccgccttacc tattacctta ctctttgcag aaaattggca actgccaacc agtctctcag
     4021 catgggcgag cgtagcatat ttaggcatta tttgtaccag cttggcaata ttgttatgga
     4081 acaaaggcat cagccaatct tcgcctattc ttgcaggtat tctactcgca cttgaaccgg
     4141 tattcggact gctaatggca ttggtctttt tatcagaaca gctcagttta gtgacttggg
     4201 ttgggattat tattgttatt tgcaccgcct tactctccgt tttgctaccg atgatgaaaa
     4261 gatacaagcg gttaatttaa caggattttt tgcaaatgaa atttccccct ctttcccacg
     4321 gcattttact tcgccgttat aaacgttttc ttgccgatat tcagttgcca aatggcgaac
     4381 aaattaccct ccattgccca aataccggtg cgatgacagg ctgtgccaac cccggtgata
     4441 cagtttggtt ttccacctct cataacccca aacgtaaata tgctcacact tgggaactaa
     4501 cccaaaccca agcgggggat tttatttgtg tcaatacctt gagagccaat cagcttgtac
     4561 aagaagcctt ggaaaatcaa tggattagcg agcttgcgga ttatcagcac attctgcctg
     4621 aacaaaaata tggcagtgaa aacagtcgga ttgatttttt gctcaaatcg gaccttctgc
     4681 cggattgttt tgtggaagtg aaatctacca cccttttaac cgaaaacggt gtgggaatgt
     4741 tccccgatgc caaaaccgag cgggggcaaa aacatttgcg ggagctgacg aaaatcgctg
     4801 aacagggctt acaagcggtc atttttttcg ctattttgca cacaggtatt gaacggtttg
     4861 aagtggcaaa gcagatagac cccaaatatg ccgaattatt cgctcaagcc attgcacaaa
     4921 aagtgcaggt tctctcctat aaaatggcaa tttcattgga aaacgggcaa cctattgcgg
     4981 caaccttaac aaagaaatgt gagttttaag cttgaaaaca cacagcatat ccccatataa
     5041 gccaaagttt ttaaaaagaa ggatttagta tgacaacgat tgtatgcgtg cgtaaagacg
     5101 gaaaagtggc tatcggtggt gatggtcagg caacgcttgg aaattgtatc gagaaaggca
     5161 cggtgcggaa agttcgccgt ttgtataaag acaaagtggt gacaggcttt gccggctcga
     5221 ccgctgatgc ctttattttg cgtgatttat tcgagaaaaa attggagtta caccaagggc
     5281 atttagtgaa atcggcagtg gaactggcaa aagagtggcg taccgagcgt gctttacgcc
     5341 gtttagaagc gatgatgatt gtcgccaatg acagcgagtt tttattggtt tcaggttcgg
     5401 gcgatgtgat tgagccggag caagatgtgc tggcgattgg ttcaggcggt aactatgcga
     5461 aagccgctgc tcttgcgtta ttacgcaccg aaaataattt atctgcaaaa gaaatcgttg
     5521 cggaagcgtt aaaaatcgcc ggcgatattg atatttacag taactacaat cacgtgattg
     5581 aagaagttta gttaaataac tctctcccct tgtgggacaa atgtttacat ttgaacgttg
     5641 gctttgccaa cggctccaca ggagcgagcg tagcgagtat tgagaccgaa aaatatttcg
     5701 ttcagaaata tttttcagag agaggggtta atgcaaaacg tttaagaaaa aagaccgctt
     5761 gttaccctct ccctgatttt tcttctgaac aaagaaaaat ctgtccctct cccacaaggg
     5821 gagagggtaa ttaatttaag gaataacatt atgtcaatga caccccgtga aatcgtctca
     5881 gaattagacg cacacattat cggacaaaaa gatgcaaaac gtgcggtagc aattgctttg
     5941 cgtaaccgct ggagaagaat gcagttgccg gaagatctcc gccaagaagt gacccccaaa
     6001 aatattttaa tgatcggtcc gaccggtgtg ggtaaaaccg aaatcgcccg ccgtttggcg
     6061 aagcttgcca atgccccttt tatcaaagtt gaggcaacca aattcactga agtggggtat
     6121 gtgggtaaag aagtggattc gattatccgt gatctagctg atgtgtcgat gaaattagtc
     6181 cgccaacaag cggtcgaaaa aaataaaatg cgtgcacaag atgcagcgga agatcgcatt
     6241 ttagacgtat tattgccacc ggcgaaagat cagtggggaa atgtgcagga atccgacaat
     6301 aattctaccc gccaaacttt ccgcaaaaaa ttgcgtgaag ggcaattaga cgataaagag
     6361 attgaaattg acgtggcagc tcaagtgagc gttgagatta tgaccccgcc gggaatggaa
     6421 gagatgacct cgcaattaca atcgctgttt gagggaatgt cgccaagcaa aaccaaaaaa
     6481 cgcaaaatga aaatcaaaga tgcgttaaag gtgatgcttg atgaagaagc ggcgaaatta
     6541 gtaaatcctg aagagctgaa acagcaagcg attgaagcgg tggaacagca cggtattgtc
     6601 tttattgatg agatcgacaa aatctgtaaa aaaggcgagc attcgggtgg agatgtttct
     6661 cgtgaaggtg ttcagcgtga tttacttcca atcattgaag gctcaacggt aaataccaag
     6721 catggtatgg tgaaaaccga ccatattctg tttatctgct cgggggcatt ccaagtggct
     6781 cgcccgtctg atttactccc agaattacag ggtcgcttgc cgattcgtgt ggagttaaaa
     6841 tcgcttacca aagaagattt tgagcgtatt ttaaccgaac cgaatgcctc attaaccttg
     6901 caatatcgtg agttgatgaa aactgaaggc gtggagattg agttcactaa agacggtatc
     6961 agccgtattg cagaaagtgc attccgtgtg aacgagaaaa ccgaaaacat cggggcaaga
     7021 cgtttacaca ccgtgcttga gcgtttgatg gacggcattt cgttcgatgc cagcgaacgc
     7081 cgtggtgaaa aaatcatcat tgatgaaaac tatgtttccg aagcgttaaa tgacgtggtg
     7141 gaaaacgaag atttaagtcg ctttattttg tagttttaag ttatacaagc ggttatttta
     7201 gcgaaaaatc ttgcgaatta gccgcttttc atattgttag ttgtaaaaat gaaaaatcta
     7261 tacaaatggc ctgctgcaca atcgctttat ccaaatcagg gaaagaagtc ttacgcaggg
     7321 aaacgttttc gttaccgtct tcgctcttgg ttgcatcatt ccaaaatcaa gcaatttgag
     7381 cagttcgtta cacaaaatcc acaactgatt ccgctcttaa atgctcgacc aaattacagc
     7441 taccccgtgg ctcaccgctt tttagataag cgtttcagtg caaaacaacg cttacaaaaa
     7501 atcacagata acctgctgtt tttaccgcaa aaattggctc atttaccacc tctatgggaa
     7561 caagcggtca attttggtga aattattgca gattttgagt tatggcttaa catcaatgaa
     7621 catcagccga tggaaggttt ttgggcatta gaacttcgcc acagaccgac taatcagcta
     7681 gtttatttat taacatttgg taagttagat gaagccttgt tgatagccgt aattcaaggt
     7741 ccgaattttg aaggttcaaa agagctggtg aaacaattaa ccaaaagttg ccacggctta
     7801 cgcccggcct atttaatggt tgaaacgatg aaggctttaa cggctgttct tggctatcag
     7861 agtttactag gcattccaca gaaataccag aataaatccc gctgggtgca aagtaagcgc
     7921 tatgtagtgg attatgatgc tatttttgcg gaatcgggcg gaaagccggg aaattattgg
     7981 cagttacccc ttgaattgga aagtaaaaaa atagatgaaa ttccaagcaa aaaacgctca
     8041 atgtatcgca agcgttatga aatgctagct aatttaaagc agaccatgga gcttatgctt
     8101 aaaacttata ctggcttaaa tgggtaaatc aaagaataca agcggtcaaa atctgcaaaa
     8161 ctttgcaaaa ctttgcaaaa aatgaccgct tacaagttac ttgccgccca tccatttatc
     8221 atagattttt tgatattcgc cattctcttt aatcgttttt aatgctgcat tgaattgatc
     8281 aactaatact tggtttttca agtgagttgc aatgcctaaa ccatcaccaa aatagtttgg
     8341 attattgatt ttctccccta caaaatctag cccttcgtgt tttcttgcca tctcagccaa
     8401 taccggcaca tcgccaaaaa tagcatcaat tcgcccattt tgcacatcta aaatagcatt
     8461 ttgataagag gcataagatt tcacattgta ctcttttttc tcttttgcta aatagtgttg
     8521 gtaagtagtc ccattttgca caccaatcgt tttcacctta gcaaaatctg tatctttttt
     8581 cgcaatgaag gcagcagagc ttggaaagta aggctcgcta aataatactt gtttcttacg
     8641 tggttccgta atacccatac ctgaaattgc agcatcaaat tgtttttgtt ttaggctttg
     8701 gattaagcta tcaaaaggtt ggctatggaa tgtacaattt gcattcatct ctttacagat
     8761 agcatttgca atatccacat caaaaccgat aatttctccc ttctcttcgg tcatttcaaa
     8821 tggaggatag cttggctcca tcacaaattt gatatcttgt gcctgcgcag taaccacaca
     8881 cccgaataaa agggtcaaaa gtgttttttt cataaaaagt ccctgtgttt tcattataag
     8941 gattaccact ttaacgcagt tactttctta aaaaaagtct tcttttcata aagtttgttt
     9001 tatgtcatac aaacacatca aattgagatg tagtttctca atcctcttga ttcctctatc
     9061 tcaaaaaaac aacccaaaag aaaaaagaa
//
LOCUS       DANLRK010000103         9076 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.103, whole genome shotgun sequence.
ACCESSION   DANLRK010000103 DANLRK010000000
VERSION     DANLRK010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9076
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.103"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            452..1456
                     /locus_tag="PXA33_001748"
     CDS             452..1456
                     /locus_tag="PXA33_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL5121060.1"
                     /translation="MPGDANKIISNGTSAGGALSTLLGASADHYDYEPYLKEAGALNA
                     SDKIFAVSAYCPITNLENADMAYEWQFNGVNEYSRIDMSRLNAAEFNDRSKPKPKPKI
                     EGSLNEAEIKVSNELAERFPTYLNSLHLVDEKGNPLTLDPKGNGSFKDYLSEVVKTAA
                     NKAYRGLVQDSEEQKAFQQISWLSFEKGKVSSVDWFGYVFSDKRMKSPPAFDALNGSS
                     GENNLFGTDTENNRHFTLYSAERSANKDLNLADPQIVKRMNPMHYLDNPNAAEHWRIR
                     VGTADRDTSLAISAILAIKLQMAGKNVNYETPWNVPHSGDYDVNELFLWADELVKGKK
                     "
     gene            1511..3361
                     /locus_tag="PXA33_001749"
     CDS             1511..3361
                     /locus_tag="PXA33_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDL5121061.1"
                     /translation="MQVKKFSQKYINWVLKLGRLKASLLGFFMVALSAILIQSSLTYL
                     FIGRVVVVDILRSIAFGLISAPFVLYFFTLLVEKLEISRLKLEKAVRDLEELREQDAY
                     LNAKLEKNSRDKSILMATISHELRTPLNGIIGLSQILLDDKTTEKQQEYLKTINMSAT
                     ALAHIFGDIIDLEKIDSSRIELVRKEVEFEQLIHDIANFGHFMAGNKQINFEVKYPPD
                     LPKYIYIDHARLSQILWNLISNAVKFTPASGRILLEIYQLDKEQFSFAVTDSGIGIPE
                     KDQRKIFDMFYQAENSKEKKAQGSGIGLSVSKRIAKLMGGDLVVASQENQGSTFTLTV
                     QAESTETKPIFAIQQHSLKVLLVEDIEMNVVVARAMLEKIGCVVDVAMSGEETFQHFA
                     QNSYDLILLDIQLPDTTGFEIAQQLRLQYENEEIDYLPLLVALTANVIQSRQEYQAQG
                     MDDVLHKPLSLDELMRCLNQHFGDDFLQKNEEKQPLVERGSENLPYSITILNELIGVM
                     GKENVLANMDLFADLMPTYLANLQRYLSDWQTTNSVESRKLTADEAHKIKGALASVGL
                     HQLQQTAQLAQSDNGEQWEQNIAHWVEMICSKWQMDLQLAKEYVRSKGDI"
     gene            complement(3457..4011)
                     /locus_tag="PXA33_001750"
     CDS             complement(3457..4011)
                     /locus_tag="PXA33_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HDL5121062.1"
                     /translation="MKSQIYYDYYPSPIGQLLLLATEEGLIYIELGQEQQTTSLDNFL
                     SAQAASGQILEIFCKTKEILDRYFAGEPLDFQQLDFLAPQGTPFQQSVWKILREIPYG
                     QTTTYGEIATQLGKPSAMRAVGGAVGRNPISILVPCHRVLGKNQALTGFGGGLPNKRF
                     LLQLEDITYKDKGIEFVKPKTKKW"
     gene            complement(4078..5559)
                     /locus_tag="PXA33_001751"
     CDS             complement(4078..5559)
                     /locus_tag="PXA33_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HDL5121063.1"
                     /translation="MKMKNFSKTLIASLVAFSSAGASAAAFQLAEISTSGLGRAYAGN
                     AAVADNASVVATNPALMTLFKQTEISAGGIFVDADVNIDGKLNATGADASHKNVIPTA
                     IVPNAYIVSPINERLSIGGGVNVNYGLISEFDPNYSAGFLGGRTSLSAVNVNFSAAYD
                     LGYGFVLGAGLNAVHAKAELGRFLGAGAARFAPAKQFLEDKKVQVQSAIQGLKALEAG
                     IAAGNTALEAQKTQVLAQVNGLLKSQNLNQTITNSAQLERLVNGIQNTNASSTIHKLK
                     GDKWALGWNVGLVYNINERNRLGFTYHSEIDLKFKGKYSNTVPVVIPKMVDTITGGAE
                     IDGTLRLRLPAFWEVSGYHKLTDKLAAQYSYKRTDWSKFQSLEAYDMQGNRLLHKTEN
                     FNDSQRIALGLSYDVNEMLTLRTGVAFDESASVTHPSISIPDTDRAWYSVGATVRFTP
                     NLSADVGYSHLRGSNLTFSEDGLATFKSKARANLYGLNVNYKF"
     gene            5907..6359
                     /gene="iscR"
                     /locus_tag="PXA33_001752"
     CDS             5907..6359
                     /gene="iscR"
                     /locus_tag="PXA33_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HDL5121064.1"
                     /translation="MKLTSKGRYAVTAILDIALHGKLEPVSLADISDRQAISLSYLEQ
                     LFAQLRRDGIVQSVRGPGGGYQLGKKPEEISVGMIISAVNENIDVTKCKGSGNCRKDS
                     QCLTHSLWERLEDQIEQFLGTISVADLAAEHADHDCEKEHCHDHPHHH"
     gene            6414..7634
                     /locus_tag="PXA33_001753"
     CDS             6414..7634
                     /locus_tag="PXA33_001753"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HDL5121065.1"
                     /translation="MKLPIYLDYAATTPMDERVAKKMMEYMTKDGIFGNPASRSHKFG
                     WEAEEAVDVARNQIADLIGADSREIVFTSGATESDNLAIKGAAHFYQTKGKHIITVKT
                     EHKAVLDTCRQLEREGFEVTYLDPEEDGILDLAKLKAAIRPDTILVSVMHVNNEMGVI
                     QPIKEIGAICRAKKIIFHVDATQSVGKIEVNVQDLNVDLMSFSSHKLYGPKGIGGLYV
                     CRKPRVRLEAIIHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMARLTVLRDR
                     LYNGFKDIEEVYVNGSMEEGKRIGSNLNISFNFVEGESLMMSLRDIAVSSGSACTSAS
                     LEPSYVLRAIGRDDELAHSSIRFTLGRWTTEEEIDHTIEIVKKAIVKLRELSPLWEMF
                     KEGVDLSKIEWNHH"
     gene            7761..8144
                     /gene="iscU"
                     /locus_tag="PXA33_001754"
     CDS             7761..8144
                     /gene="iscU"
                     /locus_tag="PXA33_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HDL5121066.1"
                     /translation="MAYSEKVIDHYENPRNVGTFDKEASDTGTGMVGAPACGDILRLQ
                     IKVNDQGIIEDARFKAYGCGSAIASSSLITEWVKGKSLEEAGAIKNSDIAEELELPPV
                     KVHCSILAEDAIKAAIADYKEKQAK"
     gene            8253..8576
                     /gene="iscA"
                     /locus_tag="PXA33_001755"
     CDS             8253..8576
                     /gene="iscA"
                     /locus_tag="PXA33_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HDL5121067.1"
                     /translation="MSITLTEAAANRVRTFLENRGKGIGLRLGVKTSGCSGLAYVLEF
                     VDVLNEDDQVFEQHGVKVIVDEKSLTYLNGTQLDFVKEGLNEGFKYTNPNVKNECGCG
                     ESFSV"
     gene            8586..>9076
                     /gene="hscB"
                     /locus_tag="PXA33_001756"
     CDS             8586..>9076
                     /gene="hscB"
                     /locus_tag="PXA33_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HDL5121068.1"
                     /translation="MNNPFTLFDLPVQFQLDNAQLSERYLALQKQLHPDNFAHKSSAE
                     QLEAVQKSADVNEALNILKDPILRAEAMIEIHTGEAKNLEEKSMRDVDFLMQQLEWHE
                     KLETIEHRKDETELTVFLKQIKAEQKAVLEQLEQTLNTADWQKANALTDKLRYFKKLI
                     IQI"
BASE COUNT         2895 a         1614 c         1938 g         2629 t
ORIGIN      
        1 tgttatggct ttattttttt aactatattg acagttataa atatcatctt gtccataaat
       61 gatcaatgat catgcaaaaa tgaaccaggc agacgcgatg agatgatcga cttcatcttt
      121 tgataaactc tttgcgattt aaacagaaaa tatatttaga atgtaaacaa acatgagtcg
      181 attttatgtt caaagagctg taataagacg gaataatgtc agaccaagat agatactttc
      241 aagtaaaatc aagcataaaa atgattttca gactttacaa atctgaaaga atgtgtaaga
      301 tcaggattgt ccgaaattcg gatggaaaat cccagtggat gtattggctg gatacatcat
      361 aataactatg tatttgtgaa tgttatttga atgattctac gtagttgatt caaaatcagc
      421 agtgcggtat ctgcatgcta atgatgaaaa aatgccgggc gatgcaaata aaattatctc
      481 taacggcacc agtgccggag gagcattatc tactttgtta ggagcttcag ctgatcatta
      541 cgactatgag ccttatttga aagaggcggg ggcattgaat gccagtgaca aaatttttgc
      601 agtttctgcc tattgcccaa ttaccaacct tgaaaatgcg gatatggcgt atgaatggca
      661 atttaatggt gtcaatgaat atagccgaat cgatatgagt cgattaaatg cagcagagtt
      721 taacgaccgc tcaaaaccaa aaccaaagcc gaaaattgaa ggctcgttaa atgaggcgga
      781 aattaaagtt tctaatgaat tagcagaacg ctttccgact tatttgaaca gtttgcattt
      841 ggttgatgag aaaggcaatc ctttaacact tgatccaaaa ggcaacggct cgtttaaaga
      901 ttatttatct gaagtggtaa aaacggctgc aaataaagcc tatagaggtt tagttcaaga
      961 tagcgaagag caaaaagcct ttcaacaaat tagttggctt tcatttgaga aaggcaaagt
     1021 cagcagtgtg gactggtttg gttatgtgtt ttcagataaa cggatgaaat caccacctgc
     1081 atttgatgcg ttaaatggaa gttcgggcga gaataattta tttggcacag acacggaaaa
     1141 taaccgccat tttacgcttt atagtgcgga acgttctgcc aataaagatc tcaatttagc
     1201 cgatccgcaa atcgtgaaac ggatgaaccc gatgcactat ttggataacc caaatgctgc
     1261 ggaacattgg cggattaggg tgggaacggc agatagagat acctcgctgg caatttctgc
     1321 aatcttagca ataaaactgc aaatggcagg caaaaatgtt aattatgaaa ccccgtggaa
     1381 tgtaccccat tcaggtgatt atgatgtaaa tgaattattc ctatgggctg atgagctagt
     1441 caaaggcaaa aagtaaaaaa tagagtaaaa taaagcagat ctgatggtct gctttttgtt
     1501 ttgaggtcgt atgcaagtta aaaaatttag ccaaaaatat attaattggg tattaaagct
     1561 agggcggtta aaagcttctc ttttaggctt ttttatggtc gctctttcgg caattttaat
     1621 tcaaagtagc ttaacctatc tttttattgg gcgagtagtc gtggtggata ttttacgctc
     1681 catcgctttt ggtttaattt ctgcgccttt tgtgctttat ttcttcacgt tattggtgga
     1741 aaaacttgaa atatctcgct tgaaattaga aaaagcagta agagatttgg aagaactccg
     1801 ggaacaagat gcctatctta acgctaaatt agaaaaaaac agccgtgata aaagcatttt
     1861 aatggcgacc attagtcacg aacttcgtac tccccttaat ggaattatcg gcttgagcca
     1921 aattttgtta gatgataaaa ccaccgaaaa acagcaagaa tatctaaaaa ctatcaatat
     1981 gagtgccact gcattggctc atatttttgg cgatattatt gatttagaaa aaattgatag
     2041 tagccgaatt gaattggtaa gaaaagaggt ggaatttgaa caactcattc acgatattgc
     2101 taattttggg cattttatgg cgggcaataa gcagattaat tttgaagtga agtatccgcc
     2161 tgatttgccg aagtatatct atatcgacca cgcacgcttg agccaaattt tatggaattt
     2221 gatcagcaat gcggtgaaat ttacccctgc aagcggtcga attttgctgg aaatttacca
     2281 attagataaa gaacagttta gctttgcggt gacggattca ggcatcggta ttccggaaaa
     2341 agatcaacgc aaaatttttg atatgttcta ccaagcggaa aactctaaag agaaaaaagc
     2401 gcaaggtagc gggattggct tatcggtctc gaaacgcatt gcgaagctaa tgggcggtga
     2461 tttggtggtg gcaagccaag aaaatcaagg ctcaactttt accctcactg tgcaggcaga
     2521 aagcacggaa accaagccga tatttgctat tcagcaacat tcccttaagg tgctattggt
     2581 agaagacatt gaaatgaatg tggtggttgc ccgagcaatg ttggagaaaa tcggttgcgt
     2641 ggttgatgta gcaatgagtg gtgaagaaac gttccaacat tttgcacaga acagctacga
     2701 tctgatttta cttgatattc aactgccgga taccaccggt tttgaaattg cacagcaatt
     2761 aagattgcaa tatgaaaacg aggaaattga ctatttaccg ctgttggttg ctttgacggc
     2821 aaatgtgatc caaagccggc aggagtacca agcacaaggt atggacgatg tgctacacaa
     2881 accgctctct ttagatgaat taatgcgttg tctcaaccaa cattttggag atgatttttt
     2941 gcaaaaaaat gaagaaaaac agccgcttgt agagagaggt tctgagaatt taccttatag
     3001 cattacaatt cttaatgaat tgattggggt aatggggaaa gagaatgtgc ttgccaatat
     3061 ggatttattt gctgatttaa tgcccactta tttggcaaat ttacagcgtt atttaagtga
     3121 ttggcaaacc acaaattctg tggaaagccg taaattaacg gcggacgagg ctcataagat
     3181 taagggagca ttagcatcag tcggattaca tcagctacaa caaactgctc agcttgctca
     3241 atccgataac ggcgagcagt gggagcaaaa tattgcacac tgggtggaaa tgatttgttc
     3301 caaatggcag atggatttgc aattagctaa ggaatatgtt agaagcaagg gggacattta
     3361 gtaaatttga aatgttacga tggtgcaagc gtccacgctt gtaccaattt taatatctga
     3421 agcacaagcg tggacgcttg tgctatcaaa acgtatttac catttcttgg ttttcggttt
     3481 tacaaattca atgcctttat ctttataggt aatatcttcc agctgtaata gaaaacgctt
     3541 attcggcaag ccaccaccga atccggttaa ggcttgattt ttccctaata ccctatggca
     3601 aggcactaaa atcgaaatcg ggtttcgccc aactgcaccg ccgacggctc gcatcgcact
     3661 tggcttgcct agttgggtgg caatttcgcc ataagtagta gtttgcccgt aagggatttc
     3721 acgcaaaatt ttccaaactg attgctggaa tggagtgcct tgcggggcaa gaaaatccaa
     3781 ctgctgaaaa tccaatggct cgccggcaaa atagcggtct aaaatttctt tggttttgca
     3841 aaaaatttct aaaatctgac cacttgcagc ttgagcggaa aggaaattat caagcgaggt
     3901 ggtttgttgc tcttgcccta gctcaatgta aatcaagcct tcttctgtgg ctaaaagcaa
     3961 taattgcccg atagggcttg ggtagtaatc gtagtaaatt tgagatttca tgtttttatg
     4021 ccaataaaaa aagaggtgta attgatttta cacctcttta aaccttaatt catcaaatta
     4081 gaatttgtag tttacgttta aaccgtaaag atttgcacga gctttgctct tgaatgtggc
     4141 taaaccgtct tcagagaagg tcaggttgct accacgaaga tgtgagtaac ctacatcagc
     4201 agataggttt ggcgtgaaac gcacggttgc acctacagaa taccaagcac gatcagtgtc
     4261 cggaattgag attgatggat gagttacact tgcactttca tcaaatgcta cacctgtacg
     4321 caatgttagc atttcattca catcataaga caagcctaat gcaatacgtt gagagtcatt
     4381 aaagttctcg gttttgtgca ataggcggtt accttgcata tcataagctt ctaggctttg
     4441 gaatttgctc cagtctgtgc gtttatagct gtattgtgca gcaagtttat cggttagttt
     4501 gtggtaacct gacacttccc agaatgccgg taggcgtaat cttagtgtac catcaatctc
     4561 tgcaccgccg gtaatggtat ctaccatctt aggaataact acaggaacgg tattagaata
     4621 tttgccttta aatttaagat caatttcaga gtggtaagta aaacctaaac ggtttctttc
     4681 attgatatta taaaccaagc caacattcca acccaatgcc catttatcgc ctttaagttt
     4741 atgaattgtt gaacttgcat tggtattttg aatgccatta accaaacgtt ctaattgagc
     4801 agaattcgtg atggtttggt ttaaattttg agattttaat aaaccgttta cttgagctaa
     4861 tacttgtgtt ttttgagctt caagagcggt attacctgct gcgatacctg cctcaagagc
     4921 ttttaaacct tgaattgcac tttgtacttg tactttcttg tcttccaaaa attgtttagc
     4981 tggagcaaaa cgtgccgcac cggcacctaa gaaacgtcct aattctgctt ttgcgtgtac
     5041 tgcattcaaa cctgccccta atacaaaacc ataacctaaa tcataagccg cactaaaatt
     5101 aacattcaca gcacttaatg atgtacgacc acctaagaaa ccagcagaat agttagggtc
     5161 aaattcagat atcaaaccat aatttacatt tacgccacca ccaatagaaa ggcgctcatt
     5221 gataggtgaa acgatataag catttggaac aatagcggta ggaatgacat ttttatgtga
     5281 ggcatctgca cctgtagcat tgagctttcc atcaatattt acatcagcat ctacgaaaat
     5341 accaccggca gaaatttcgg tttgtttaaa taacgtcatt aacgcagggt tggttgcaac
     5401 aacagaagcg ttatcggcta cagcggcatt acctgcgtaa gctctaccta aaccggatgt
     5461 tgaaatttca gctaattgga atgcggcagc agatgcaccg gctgagctga atgctactaa
     5521 tgaagcgatt aaagttttgc taaagttttt cattttcata ggaaatctct taaaaaatat
     5581 atattttaga atgaataatt cataaaagtt ataagaagaa agtgcagatt ttatgtgtcg
     5641 aagtactctc tatcaattaa atttcaactg ttccgatcag ttaggagcaa ataagagtga
     5701 aattgtgcgg tctatgaact gtttatcttt ggtattttgg tattttcggc tcttttttcg
     5761 tataatcagc gttattgttt taatctgttt taatatataa aaccttttac actaattagt
     5821 cctaaatgat tgactaaatt ggtggggtat atcaaaattt agcaactgtt tcagaagatt
     5881 ttctggataa tcaatgagga tattttatga agcttacctc aaaagggcgt tatgcagtaa
     5941 ccgccatttt agatattgct ttacacggaa aactagagcc ggttagctta gccgatattt
     6001 cagatcgcca agcgatttca ctgtcttatt tagagcaact gtttgctcaa cttcgccgtg
     6061 acggtatcgt gcagagcgtt cgtggaccgg gtggtgggta tcaattaggt aaaaaaccgg
     6121 aagagattag cgtgggtatg atcatctccg ccgtgaatga aaatattgat gtcaccaaat
     6181 gtaaaggtag tggtaattgc cgtaaagatt ctcagtgttt aacgcattct ttgtgggagc
     6241 ggttagaaga tcaaattgag cagtttttag ggacaatttc agttgcggat ttagccgcag
     6301 aacacgctga tcacgattgt gaaaaagagc attgtcacga tcatcctcat catcactaat
     6361 tgcaaatttt ttatcaaaaa tgaccgcttg ggttgtttta actaggagta acaatgaaat
     6421 taccaattta tttggattac gcagcaacca caccaatgga cgaacgtgtt gcgaaaaaaa
     6481 tgatggaata tatgaccaaa gacggtatct ttggtaaccc tgcttctcgc tcacacaaat
     6541 ttggttggga agcggaagaa gcggttgatg tggctcgtaa ccaaattgcc gatttaatcg
     6601 gtgcagacag ccgtgaaatc gttttcactt ctggtgcaac agagtcggat aacttagcca
     6661 tcaaaggtgc ggctcatttc tatcaaacca aaggtaaaca cattatcact gttaaaaccg
     6721 aacataaagc agtattagat acttgccgtc agttagagcg tgaaggtttt gaagtgactt
     6781 acttagaccc tgaagaagac ggtattttag atttagctaa attaaaagcg gctattcgtc
     6841 cggacacaat tttagtttcg gtaatgcacg taaataacga aatgggcgta atccaaccga
     6901 ttaaagagat cggtgcaatc tgccgtgcga agaaaatcat tttccacgtt gatgcgaccc
     6961 agtctgttgg taaaatcgaa gtgaatgtac aagacttaaa tgttgattta atgtctttct
     7021 caagccacaa attatacgga ccaaaaggta tcggcggtct atatgtttgt cgtaaaccac
     7081 gtgtgcgttt agaagcaatt attcacggtg gcggtcacga gcgtggtatg cgttcgggta
     7141 cattgcctgt tcaccaaatt gtaggtatgg gtgaagcata tcgtatcgct aaagaagaaa
     7201 tggcaacgga aatggctcgt ttaaccgtgt tacgtgatcg tttatataac ggctttaaag
     7261 atattgaaga agtatatgta aacggttcaa tggaagaagg caaacgtatc ggttcaaacc
     7321 taaacatcag ctttaacttt gttgagggcg aatcgttaat gatgtcatta cgtgatatcg
     7381 cagtatcatc aggttcagct tgtacttctg caagtttaga gccatcttat gtgttacgtg
     7441 caatcggtcg tgacgatgaa ttagctcaca gctcaatccg tttcacttta ggtcgttgga
     7501 cgactgaaga agaaatcgat catactattg aaatcgtgaa aaaagctatc gtgaaattgc
     7561 gtgagctttc gcctctttgg gaaatgttca aagaaggtgt agatttgtct aaaatcgaat
     7621 ggaatcacca ctaattcaga aaaattttgc aagggcaaat aatatttgcc cctacaaaat
     7681 aagctttcat cacaaatagt aaattgaatg taggggcgaa ataggtttcg ccggaaatag
     7741 gaaataaaag gaatacaaaa atggcatata gcgaaaaagt aattgatcat tacgaaaacc
     7801 cacgcaacgt agggactttt gataaagaag catcagacac cggtacaggt atggtgggag
     7861 caccggcttg tggtgatatt ttacgtttac aaattaaagt gaacgatcaa ggtatcattg
     7921 aagatgcacg ctttaaagca tacggttgtg gttctgcaat cgcatcaagt tctctcatta
     7981 ccgaatgggt aaaaggtaaa tcattagagg aagcaggtgc gattaaaaac agtgatatcg
     8041 ctgaagagtt agaattacca ccggtaaaag ttcactgttc aatcttagca gaagatgcaa
     8101 tcaaagctgc aattgcagac tacaaagaga aacaagctaa gtaatatgat acaatcaatg
     8161 caagcggtcg atttttgcaa aaaatttgca aaatttaacc gcttgcattt gctatttcta
     8221 tcttgattta atccattcac ataaggaaaa ctatgagcat taccttaaca gaagctgccg
     8281 caaaccgagt tcgtacattc ttagaaaaca gaggtaaagg tatcgggcta aggcttggtg
     8341 tgaaaacttc cggttgttcg gggcttgcct atgtgcttga atttgttgat gtattaaatg
     8401 aagacgatca ggttttcgaa caacacggcg taaaagttat tgtcgatgaa aaaagcctga
     8461 cttacttaaa cggtacgcag ctggattttg tgaaagaagg cttaaacgaa ggctttaaat
     8521 acaccaaccc gaatgttaaa aacgaatgcg gctgtggcga aagttttagt gtgtaaggaa
     8581 ctgtaatgaa taaccctttt actttatttg atttacccgt tcaatttcaa cttgataacg
     8641 cccagctttc tgagcgttat cttgctttac agaaacagct tcacccagat aactttgccc
     8701 acaaatcaag tgctgaacag ttagaagcgg tgcaaaaatc tgctgatgtc aatgaggcac
     8761 tcaatattct taaagatccg attttacggg cagaagcgat gattgaaatc catacaggtg
     8821 aagccaaaaa tctcgaagaa aaaagtatgc gagatgtgga tttcttaatg cagcaactcg
     8881 aatggcacga aaaactggaa accattgagc atcgtaaaga cgaaaccgaa ctgaccgtct
     8941 ttttaaaaca gattaaagcg gaacaaaaag cagtattaga acagcttgaa caaacgttaa
     9001 atacagccga ctggcaaaaa gccaatgcgt taaccgacaa gctccgttac tttaaaaaat
     9061 taattattca gattga
//
LOCUS       DANLRK010000104         8925 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.104, whole genome shotgun sequence.
ACCESSION   DANLRK010000104 DANLRK010000000
VERSION     DANLRK010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8925
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.104"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            168..1358
                     /gene="malE"
                     /locus_tag="PXA33_001757"
     CDS             168..1358
                     /gene="malE"
                     /locus_tag="PXA33_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005537801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HDL5121069.1"
                     /translation="MKNKCVKFTLATLATLVLSGTVMAKMTEGKLVIWINGDKGYNGL
                     AEVGKKFEKDTGVQVLVEHPDRLEEKFAQVASTGDGPDIMFWAHDRFGGYAQSGLLAE
                     VNVSKEFKDKFVDFAWDAETYNGKIIGYPVAIEAISLIYNKDLVKEAPKSWEEIIELD
                     KKLKKEGKNAIMWNLSEPYFTWPIAASNGAYAFKFANGKYDPKDIGVNNEGAVKALQF
                     VVDMVKNKHISADMDYAVAEASFNKGQSALTINGPWSWGNIDKSGIKYGVTLLPTLNG
                     QASKPFVGVLSAGVNSSSPNKDLAKEFLENYLLTDDGLDAVNKDKPLGAVALKSYQEK
                     LAADPRIAATMANAKNGEIMPNIPQMSSFWYAEKSAINNAVTGRQSVKEALDDAHSRI
                     QKQQ"
     gene            1477..3015
                     /gene="malF"
                     /locus_tag="PXA33_001758"
     CDS             1477..3015
                     /gene="malF"
                     /locus_tag="PXA33_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HDL5121070.1"
                     /translation="MLTQTQSKSTHWLKYLLAGLVLLLDFYLVVLMYSQGEYLFAILT
                     LIILTSGVYIFTNKNTYAWRYVYPGITGMAIFILFPLVATIAIAFTNYSGSNQLSFER
                     AVSVLTEQRYFAGDKYQFTLYPQADNKYQIALTNPTTEQTFVSEPISLATGTNVVVTE
                     QNRPTGEVAPLKVITQNRAALQSMKVVLPNNNELTMSSLRQFSEQKPRYQFDEENKIL
                     LNNETGKRYQANDETGFFQSIDENGHWQSETLEPGYTVTSGFNNFIKIFTDEGIQKPF
                     VQIFIWTVIFSLLTVVFTVILGMVLACLVQWEALKGKAVYRVLLILPYAVPSFISILI
                     FKGLFNQSFGEINMILNQLFGISPEWFNDPLLAKVMILIVNTWLGYPYMMILCMGLLK
                     AIPADLYEASAMDGASTWQNFSKITFPLLLKPLTPLMIASFAFNFNNFVLIQLLTNGR
                     PDMIGTTTPAGYTDLLVSYTYRIAFEGSGTQDFGLAAAIATIIFLLVGGLALLNIKAT
                     KMEL"
     gene            3068..3958
                     /gene="malG"
                     /locus_tag="PXA33_001759"
     CDS             3068..3958
                     /gene="malG"
                     /locus_tag="PXA33_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HDL5121071.1"
                     /translation="MAIVQSKSVRYRVWATHFILICFLALIIFPLLMVIGISLRPGNL
                     AIGDIIPSQISWEHWQAALGFDVTHADGTVTPPPFPVLRWLWNSIKVATITSIGIVTL
                     STTCAYAFARMKFKGKKTILQGMLIFQMFPAVLSLVALYALFDRLGQYVPFLGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDGSLEEAASLDGATPWQAFRLILLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLYPQNYLWGDFAAAAVLSAIPITL
                     VFLLAQRWLIGGLTAGGVKG"
     gene            4033..6090
                     /locus_tag="PXA33_001760"
     CDS             4033..6090
                     /locus_tag="PXA33_001760"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HDL5121072.1"
                     /translation="MKNALPYLCLFYTSSLFAQSWQHSHFSDFNDDLEKHFFQSQVQL
                     EKGSYPLRFKFGEQCYQPQQAIKLNQSVALVPCINEAPQLRLFRQGNYLAQIDLRSGT
                     PTPTISVEQQLQHNEAMFQSCPNWDKKPIEIDVSSTFAEGESVSDFYSGNTAIVKNGK
                     VTLMPNENASGLILLEKSNTENTATFDWKNATVYFVLTDRFYNGDPTNDHSYHRQKDG
                     MQEIGTFHGGDLKGLTEKLDYLQQLGVNVLWISSPLEQMHGWVGGGDKGDFPHYGYHG
                     YYHLDWTKLDANMGTEDDLSRFVQQAHQRGIRVLFDVVMNHTGYATLADMQEFNFGGF
                     YLTPDEISRTLGEKWTHWKPKSGQNWHSFNDFIRFNDNQAWQNWWGKEWVRADIADYD
                     SPKFDDLKMSLAALPDLKTESEQAVKLPEFFANKNTNAKTLPNAKVRDYLIGWLSDWV
                     RKYGIDGFRVDTAKHVEKSTWLVLKQSAQQALNEWQKANPNAGFNDRFWMTGEAWGHG
                     VFKSDYYQNGFDAMINFDFQDQAKQGLNCFAHIEPIYAEMNRKLSDFNVLSYLSSHDT
                     RLFFHSDSEQNIEKHKTAANLLLLSPGAVQIYYGDESGRTFGATGSDLIQGTRSDMNW
                     QELQQDPQKQALHQHWQKLTQFRQRHPAIGAGVHQLIKNDSYFAFKRVLNEDKVMVVW
                     AGN"
     gene            complement(6204..8882)
                     /gene="malT"
                     /locus_tag="PXA33_001761"
     CDS             complement(6204..8882)
                     /gene="malT"
                     /locus_tag="PXA33_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005594368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HDL5121073.1"
                     /translation="MPRKRLIQELDKSCSYPVVLINAPAGYGKTTLVSQWIENKKNIG
                     WYALDEGDNNSDRFATYFSAALHSAINEEIDVLLEENRKANLLALFNQLLIKAAAFSE
                     HFYLAIDDYHIIENDEIHEALKYWIKHQPSNMTLILISRSIPPLSIASLRVQEQLLEI
                     DINQLMFDHQESTAFFQARLGSELNQQDVIALCNEVEGWPTALQLISLFAKNKSHTLQ
                     VPLQDIAKRLAKSNHFHINEYLADEVLNKVDEETRLFILRCSVLHSMNESLVQAVTGE
                     QNSRTKLESLEKQGLFLQQMANSKWQSSDDSWWKFHPLFASFLNLCCQNELYAELPQL
                     HQKAANAWLQLGYVTEALHHAMQLSDTGLLLNILSKHAWTVFHQGELKLLEESLNCLD
                     YAHLTEHTNLVLLKAWLVQSQHRHIEVGGILADFSRALTENKIELSKTANAEFNVLRA
                     QVAINSGDENTALQLASEALNDLSENAYYAHIVATSIIGEAHHCHGNLAEALAMLQKA
                     ERMARQHHTYHNILWSQLQQSEILLAQGFSQAAYEMLDKASEFVKENHLQKVPMYEFL
                     LRSKGKILWEWYNLDKAESMAVAGMNALSKFEDKLQCLTLLTKISLARGNLDNTARLL
                     GEVEQLEHSHSYHHDWIANADQVRIFYWQMTNDVSAVRNWLIQNPSPVSDKNHFTQVQ
                     WRNIARSRILLEEYHEAKAILDNLIETAEKFSLISDLNRALIVRNRLYFLQGEKELAQ
                     QDLIQALKLTRQTNFISAFVIEGDVMAQQIRHLLQLNVLDELVLHKAQFILRNINQFY
                     RQKFAHFDENFVNQLLKNPKVPELLKISPLTQREWQVLGLIYSGYSNEQISDELQVAA
                     TTIKTHIRNLYQKIGVTNRNEAISYTKELLMLMGYH"
BASE COUNT         2668 a         1796 c         1767 g         2694 t
ORIGIN      
        1 aagcagatca caaaatgata aaaaatgtta cttagatcat acttttagct tggggggcgt
       61 agagcaaagg gtgatctcaa atagattgta tagcccataa tttgccataa tttaataaat
      121 gggttgtttc atatccgttt cttttattta acttcaagga gttttttatg aaaaacaaat
      181 gtgtcaaatt caccttagct actcttgcta ccttagttct ttcaggaaca gtaatggcaa
      241 aaatgacgga aggtaaactc gttatctgga ttaacggtga taaagggtac aatgggcttg
      301 ccgaagtcgg taagaaattt gaaaaagata ccggtgttca agttttagtt gaacacccgg
      361 atagacttga agaaaaattt gcacaggttg cctcaactgg tgacggtccg gatattatgt
      421 tctgggctca cgaccgcttt ggtggttatg cacaatccgg tttattggct gaagtgaatg
      481 taagcaaaga atttaaagat aaatttgtcg attttgcttg ggatgctgaa acttacaacg
      541 gcaaaattat cggttatccg gttgcgattg aagcgatttc attaatttat aataaagatt
      601 tagtgaaaga agcaccaaaa tcttgggaag aaattattga gttagataaa aaactcaaaa
      661 aagagggcaa aaatgccatt atgtggaact tatccgagcc ctatttcacg tggccgattg
      721 cggcttcaaa cggagcttat gcattcaaat ttgctaacgg caaatacgat ccaaaagata
      781 ttggtgtgaa caatgaaggg gcagtaaaag cactacaatt tgtagtcgat atggtgaaaa
      841 ataaacatat tagtgcggat atggactatg ccgttgccga agcctccttt aataaaggac
      901 aaagtgcctt aaccatcaat ggtccttggt cttggggcaa tattgataaa agcggtatta
      961 aatatggtgt aactttactc ccaacattaa acggtcaagc ttctaaacca ttcgtagggg
     1021 tgttaagtgc cggtgtaaac agctcaagcc caaataaaga tttagccaaa gaattcttag
     1081 agaattattt actcaccgac gacggtttag atgcagtcaa taaagataaa cctttaggtg
     1141 cggtggcgtt aaaatcttac caagagaaat tagcggctga tcctcgtatt gctgcaacga
     1201 tggcaaatgc gaaaaacggt gaaattatgc caaatattcc gcaaatgtct tctttctggt
     1261 atgccgaaaa atctgctatc aataatgcag tgaccggtcg tcaatcggtg aaagaagcat
     1321 tagatgatgc acattcacgc attcaaaaac aacaataatt cttcatcatt ctgagtgggt
     1381 tattatgacc cactctattt ttagattcac cctaaataca ataaaagtac agtaaaagta
     1441 ccgtaacaaa atgcagtaaa aattcgaggt gctgttatgc taactcaaac tcaatccaaa
     1501 tccactcatt ggctcaaata tctcttagcc ggattagtct tgctccttga cttctatctt
     1561 gttgtcttaa tgtactcaca aggtgaatat ctttttgcta ttttaacgct gattattcta
     1621 acttccggtg tctatatttt taccaataaa aatacctatg cttggcggta tgtttaccct
     1681 ggcattaccg gaatggcaat ttttattttg tttccgctgg ttgcgactat tgcaattgcg
     1741 tttaccaact atagtggttc taatcaatta tcctttgaac gagcggtatc cgtcttaact
     1801 gaacaacgct attttgccgg agataaatat caattcaccc tttatccgca agcagataat
     1861 aaatatcaaa ttgcattaac caatccgacg acagagcaaa cttttgtgtc agagccgatt
     1921 tcattggcta ccggaacgaa tgtggttgta accgagcaaa acagaccaac tggcgaagtc
     1981 gctcccctta aagtgattac gcaaaatcgg gccgcacttc aatcaatgaa agtggtttta
     2041 ccgaataata atgaactgac aatgagctca ttacgtcaat tctccgagca aaaaccacgc
     2101 tatcaatttg atgaagaaaa taaaatatta ctcaataatg agactggcaa acgttatcaa
     2161 gcaaatgacg aaaccggttt cttccaatcg attgatgaaa acggtcattg gcaaagcgaa
     2221 acacttgaac caggttatac tgtcacatca ggctttaata acttcattaa aatttttacc
     2281 gatgaaggta ttcaaaaacc ttttgtccaa atatttattt ggacggtgat cttctcttta
     2341 ttaaccgttg tctttaccgt tattctcggt atggttcttg cctgtttggt acaatgggag
     2401 gcattaaaag gtaaagcagt ctatcgtgtc ttattgattt taccttatgc cgtaccatca
     2461 tttatttcga ttctgatttt taaaggacta tttaaccaaa gttttggtga gatcaatatg
     2521 attttaaacc aactattcgg catcagccct gaatggttta acgatccgct ccttgccaaa
     2581 gtgatgatct taattgtcaa tacgtggtta ggttatcctt atatgatgat tttatgtatg
     2641 gggttactca aagctattcc ggctgattta tatgaagcct ccgcaatgga cggtgcttcc
     2701 acttggcaaa acttcagtaa aatcactttc ccattactgt taaaaccact tactccgtta
     2761 atgattgctt cgtttgcatt taactttaat aactttgtat taattcaact attaacaaac
     2821 ggtcgcccgg atatgattgg aacaaccact ccagccggtt ataccgattt attagtcagc
     2881 tacacctatc gtattgcctt tgaaggaagt ggtacgcaag atttcggctt agcagccgcc
     2941 atcgcaacca ttattttctt attagtcggt ggcttggcac tcttaaatat caaagcaacc
     3001 aaaatggaat tataaaagcg ggagggattt aacaatcccg accccaaact aactttagga
     3061 gttatatatg gcaattgttc aatctaaatc tgtgcgttat cgggtatggg caacccattt
     3121 catcttaatc tgtttccttg ccttaattat tttcccttta ttaatggtga ttggcatttc
     3181 gttacgaccg ggaaatctgg ctatcggtga tattattcca agccaaattt cgtgggaaca
     3241 ttggcaagcc gctcttggtt ttgatgttac acacgccgat ggtacagtca caccgccgcc
     3301 attcccggtg ctacgctggt tatggaactc catcaaagtg gcaacaatta cctctatcgg
     3361 cattgtaacc ttatctacga cctgtgctta tgcatttgcc cgaatgaaat ttaaaggcaa
     3421 aaaaaccatt ctacaaggta tgctgatttt ccaaatgttc ccggcagtgc tttccttagt
     3481 tgccttatat gcgttgtttg atcgtcttgg gcagtatgta ccgtttcttg gtttaaacac
     3541 acacggtggt gttatttttg cctacttagg cggaattgcc ttacacgttt ggacaatcaa
     3601 aggctacttt gaaacgattg atggttcatt ggaagaagcc gcttcccttg atggtgcaac
     3661 cccgtggcaa gcgttccgat taatcttatt accgctttca gtgccgattt tggcggtagt
     3721 tttcattctt tcatttattg ccgccattac cgaagtaccg gttgcctcac tcctattgcg
     3781 tgatgtaaac agctacaccc tagccgttgg tatgcaacaa tacctctatc cgcaaaacta
     3841 tttatggggt gacttcgcag cagcggcggt cttatccgca attccgatta cgctggtctt
     3901 cttattggca caacgctggt taatcggtgg tttaactgcc ggcggcgtga aaggctagtt
     3961 tgcaaaaaat tctctaaaaa cgaccgcttg tcgagctgat actgcggtcg tcaatcttta
     4021 ttagggctgt ctatgaaaaa tgcgttacct tatctctgct tattctatac ctcaagcctt
     4081 tttgcccaat cttggcaaca ttcccatttt tctgatttta acgatgatct cgaaaaacat
     4141 ttttttcaat cccaagttca attggaaaaa gggagctatc cgcttaggtt caaatttggc
     4201 gaacaatgtt atcaacccca acaagcgata aaattaaatc aatcggttgc gttagtccct
     4261 tgtataaatg aagctccaca actgcggttg tttcgtcaag gcaactatct ggcacaaatt
     4321 gatctgcgaa gcggcactcc gacacccaca atcagcgtag aacagcagct acagcacaat
     4381 gaagcgatgt ttcaaagttg cccgaattgg gataaaaaac cgattgaaat tgatgtttct
     4441 tccacttttg ccgaaggcga atcggttagc gatttttatt caggcaacac cgctatcgtt
     4501 aaaaacggca aagtcacctt aatgcccaat gaaaacgcaa gcggtctgat tttgctggaa
     4561 aaaagcaata ccgaaaatac ggcaacgttt gattggaaaa atgccaccgt ctattttgtg
     4621 ctgaccgacc gcttttataa cggcgatccg accaatgacc acagctacca ccgccaaaaa
     4681 gacggaatgc aagaaatcgg cacctttcac ggtggtgatt taaaaggatt aaccgaaaag
     4741 ctcgattacc tacaacaact tggggtaaac gtattgtgga ttagctctcc gttagaacaa
     4801 atgcacggct gggttggcgg cggtgataag ggggattttc cgcattatgg ctatcacggt
     4861 tactatcatt tagattggac gaaattagat gccaatatgg gtactgaaga cgatttaagt
     4921 cgctttgttc aacaagccca ccagcgtggt attcgggtgc tatttgatgt ggtgatgaac
     4981 cataccggct acgcgacgct tgccgatatg caggagttta actttggcgg attttactta
     5041 acaccggacg aaattagccg cacattaggc gaaaaatgga cacactggaa gccgaaatca
     5101 ggacaaaatt ggcacagttt caatgatttc attcgcttta acgataatca ggcttggcaa
     5161 aattggtggg gaaaagaatg ggttagagct gatattgccg attacgacag ccctaaattt
     5221 gatgatttaa aaatgtcgct cgctgccctt cctgatctaa aaaccgagag cgaacaagcg
     5281 gtcaaattac ctgaattttt tgcaaataaa aataccaatg ccaaaacatt accaaacgcc
     5341 aaagtgcgtg attatttaat tggttggtta tccgattggg tcagaaaata tggcattgac
     5401 ggttttcggg ttgataccgc caaacacgtt gaaaaatcca cgtggctggt attaaaacaa
     5461 tcggcacaac aagccttaaa cgaatggcaa aaagccaatc cgaacgccgg ttttaacgat
     5521 cgcttttgga tgacaggaga agcgtgggga cacggcgtgt tcaaaagtga ctattatcaa
     5581 aacggttttg atgcaatgat caatttcgat tttcaagatc aggctaaaca agggttaaat
     5641 tgctttgccc atattgagcc gatttatgcc gaaatgaatc gcaaattaag cgacttcaat
     5701 gtgttgagct atttatcttc acacgatact cgattatttt tccattcgga tagtgaacaa
     5761 aatatcgaaa aacacaaaac cgccgcaaat ttgctgttgc tttcacccgg tgcagtgcaa
     5821 atctattatg gtgatgaaag tgggcggaca tttggtgcga ccggttcaga cctgatacaa
     5881 ggcacccggt cggatatgaa ttggcaagag ttgcagcaag atcctcagaa acaggcattg
     5941 catcaacatt ggcaaaaatt gacacaattc cgccaacgcc atccggctat tggcgcaggt
     6001 gtccatcaac tcatcaaaaa tgatagctac tttgcattta agcgtgtgtt gaatgaagat
     6061 aaagtgatgg tggtttgggc cggaaattaa atctacttta gcttcaagtt ttctctcttc
     6121 tggggaaaac ttgaagccgg ttcggggaaa caagttgcaa aaaattacta aaaatagacc
     6181 gcttgttccc catgagcttg tgatcaatga taccccatca acatcagcag ttctttcgta
     6241 tagctgattg cttcgttacg attcgtcaca ccgattttct gataaagatt tcggatatgg
     6301 gttttaatcg tggttgcagc cacttgcaat tcatctgaaa tttgttcatt gctatatccg
     6361 gagtaaatca aacccaatac ttgccattcc cgctgagtga gcggactaat ttttaagagt
     6421 tccggcactt tcggattttt caataattga ttcacaaagt tttcatcaaa atgggcaaat
     6481 ttttggcgat aaaattgatt gatgttgcgt aaaataaact gtgctttgtg caataccagt
     6541 tcatctagca cattaagttg caataaatgg cggatctgct gtgccattac atccccttca
     6601 atcacaaacg cactgataaa attcgtttgg cgggtgagtt ttagtgcctg aattaaatct
     6661 tgctgagcca attctttctc gccttgtaaa aaataaagcc ggttacgaac aattaatgcg
     6721 cgatttaaat cgctaatcag agaaaatttt tcagccgttt caattaaatt atctaaaatg
     6781 gctttcgcct cgtgatattc ctcaagcaag attcgagacc gtgcaatatt tcgccattgc
     6841 acttgggtaa aatgattttt atctgaaacc ggtgaggggt tttgaattaa ccagttcctc
     6901 accgcagaaa cgtcattggt catctgccaa taaaatatac gtacctgatc ggcgttggca
     6961 atccaatcgt ggtgataaga atgagaatgt tctaattgtt cgacctcgcc taataatcgg
     7021 gcagtgttat ccaaattgcc tcgagccaac gagatcttcg tgagtaaagt taagcattgt
     7081 aacttatctt caaatttaga taaggcattc attccggcaa ctgccattga ttcagcttta
     7141 tctaaattat accattccca taaaatcttg cctttagaac gtagcaaaaa ttcatacatc
     7201 ggcacttttt gtagatggtt ttctttcaca aactcgcttg ctttatctaa catttcatag
     7261 gctgcttgtg aaaacccttg ggcaagcaaa atttcagatt gttgaagctg tgaccacaaa
     7321 atattgtggt aagtatgatg ctgacgtgcc atacgctctg ctttttgcaa cattgctaat
     7381 gcttctgcca gattgccgtg gcaatgatga gcttcgccaa taatagacgt tgcaacaata
     7441 tgagcataat aggcattttc tgaaagatcg tttaacgctt cagacgctaa ttgaagtgcg
     7501 gtattctcat caccggaatt gatagcaact tgtgcccgta gcacattaaa ttccgcattg
     7561 gcagttttgc tcaattcaat tttattttca gttaatgcac gagaaaaatc cgctaaaata
     7621 ccacctactt caatatggcg gtgctggctt tgtaccaacc aggctttgag taacaccaaa
     7681 ttagtatgtt cggttaaatg ggcgtaatct aagcaattta agctctcttc caataatttg
     7741 agttctcctt gatgaaacac cgtccaagcg tgtttgctca aaatattgag tagtaatccg
     7801 gtatccgata actgcattgc gtgatgtaag gcttctgtaa catagcctaa ttgtaaccac
     7861 gcattcgccg ccttttgatg cagttgcggt aattcagcat ataactcatt ttggcaacat
     7921 aaatttaaaa aggaagcaaa aagcggatgg aatttccacc agctatcatc actactctgc
     7981 cacttactgt ttgccatttg ttgtaaaaac aaaccttgct tttcgagaga ctctaatttg
     8041 gtgcgactgt tttgctcccc tgtaacagcc tgcactaaag actcattcat tgaatgcaaa
     8101 accgaacaac gcaaaataaa caaacgagtc tcttcatcga ctttattaag cacttcatcg
     8161 gctaaatatt cgttaatatg aaaatggttt gatttggcta aacgtttggc aatatcttgc
     8221 agtggtactt gtagcgtgtg tgatttattt tttgcaaaaa ggctaattag ctgaagggct
     8281 gtgggccacc cttcaacttc gttacacagt gcgataacat cttgctgatt taactccgaa
     8341 cctaatcttg cttgaaaaaa agcggtactt tcttggtgat caaacattaa ctggttaata
     8401 tcaatttcca ataattgttc ttgcactctt aaactggcaa tacttaacgg gggaattgat
     8461 cgagaaatca agataagcgt catattagac ggttgatgtt taatccaata ttttaatgct
     8521 tcgtgaattt catcattttc aataatatga taatcatcaa tagcaagata aaaatgctct
     8581 gaaaatgccg cggctttaat caataattga ttaaataaag ctaataaatt agctttacga
     8641 ttttcctcta acaatacgtc tatttcttca ttgattgctg aatgcaatgc cgcactaaaa
     8701 taagtggcaa aacgatctga attattatcg ccttcatcta acgcatacca gccgatattc
     8761 tttttatttt caatccattg ggaaaccaga gtggttttgc cataacctgc cggtgcattt
     8821 atcaatacga ccggataact gcaagatttg tctaattctt gaattaaacg ttttcgagga
     8881 atgctgtttt gtaatcgaaa agaacaaaca agttttgatg gtatt
//
LOCUS       DANLRK010000105         8774 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.105, whole genome shotgun sequence.
ACCESSION   DANLRK010000105 DANLRK010000000
VERSION     DANLRK010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8774
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.105"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            <1..697
                     /gene="artP"
                     /locus_tag="PXA33_001762"
     CDS             <1..697
                     /gene="artP"
                     /locus_tag="PXA33_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007523332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HDL5121074.1"
                     /translation="SNQALFDINLEIEKGDTVVLLGPSGAGKSTLIRTLNLMEVPQSG
                     TLAIANHQFDLTAKTDSKAVSLLRREVGMVFQQYHLWPHLTVMQNLIEAPMKVLGLSK
                     EEAVARAKGHLARLRLSEFAERFPLHLSGGQQQRVAIARALMMQPQVLLFDEPTAALD
                     PEITAQVVDIIKELQSTGITQVIVTHEVGVARKVASKVVYMEKGTIIEQGDASCFESP
                     KTEQFAQYLSHSE"
     gene            723..1448
                     /locus_tag="PXA33_001763"
     CDS             723..1448
                     /locus_tag="PXA33_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDL5121075.1"
                     /translation="MKKLLLASAIAFATLSANAQETITFAMEPSYAPFETTNEKGEVI
                     GFDVDIAKALCEELKATCEFKTQSFDSLIAGLKFKNFDAIISGMDITEARQKQVAFTN
                     AYLENSVSYVAMKGKFTPETAKNVGVQNGTTFQQYLSKNAKQYNLKPYASLQSAVLDL
                     KNGRVDMVFGDTPVLAEWLKTEKELDFVGEKVVDKAFFGNGYGIAVNKSNTALLEKLN
                     SALATIKTNGKYEQIYNTWMAGK"
     gene            1448..2119
                     /gene="artQ"
                     /locus_tag="PXA33_001764"
     CDS             1448..2119
                     /gene="artQ"
                     /locus_tag="PXA33_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HDL5121076.1"
                     /translation="MSSDILNLLVNATLMTLGIALASLLIGMLLSIFFVVLETSKIAC
                     IRKPTSIIITLLRGLPEILVVFLIYFGLIQVIFLITDEFAELNAFWCGAIALGIIFAA
                     YASQSLRGAIKAIPVGQWESGTALGLSRAQVFVRIILPQVWRHALPGLSNQWLVLLKD
                     TALVSLIGVDDLMRQSELINANTHQPFTWYGIAALIYLAITLISQRVIKVLEGRFTRF
                     ERGVA"
     gene            2119..2802
                     /gene="artM"
                     /locus_tag="PXA33_001765"
     CDS             2119..2802
                     /gene="artM"
                     /locus_tag="PXA33_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HDL5121077.1"
                     /translation="MWWDYLVEISQGIGTSLQLTFVALIIAFILAIILTFILSLENRP
                     LKWLVNGFITLFTGTPLLIQFFLIYSGPGQFEWITESAAWVLLSDAWFCAMLALALNS
                     AAYSTLLFHGAVKAIPKGQWETCAALGLSRLDTLKILVPYALKRALPSYSNEIVLVFK
                     GTSLASTITIMDIMGYARQLYGTEYDALSIYGMAGVIYLVITGIMTIVLRKLESRVLA
                     FERFETEKA"
     gene            complement(2901..3905)
                     /gene="ruvB"
                     /locus_tag="PXA33_001766"
     CDS             complement(2901..3905)
                     /gene="ruvB"
                     /locus_tag="PXA33_001766"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HDL5121078.1"
                     /translation="MIEADRIISASPKREEEVIDRAIRPKLLADYVGQPSVHDQMEIF
                     IKAAKLREEALDHLLIFGPPGLGKTTLANIVANEMGVNIRTTSGPVLEKAGDLAAMLT
                     NLEPYDVLFIDEIHRLSPAIEEVLYPAMEDYQLDIMIGEGPAARSIKLDLPPFTLVGA
                     TTRAGSLTSPLRDRFGIVQRLEFYSVDDLTLIVKRSADCLNLNLSADGAYEVARRSRG
                     TPRIANRLLRRVRDYADVRNNGIITSDIAKQALAMLDVDSEGFDFMDIKLLQAIVERF
                     DGGPVGLDNLAAAIGEERDTIEDVLEPYLIQQGFLQRTPRGRIATSRTYAHLGITQID
                     "
     gene            complement(4042..4647)
                     /gene="ruvA"
                     /locus_tag="PXA33_001767"
     CDS             complement(4042..4647)
                     /gene="ruvA"
                     /locus_tag="PXA33_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005686751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HDL5121079.1"
                     /translation="MIGRLHGKIIEKQPPEILLDVQGVGYELLLPMTSFYSLPQIGEE
                     ATIFTHLVVREDAHLLFGFAQKQDRTLFRELIKTNGVGPKLALAILSAMSVSQFATAI
                     ENEELAKLTKIPGIGRKTAERLLVELKGKFKGMAQSDFFVEVSHEQIVASNTPDPANE
                     ALDALIALGYKPADAEKMIKKVNKSGATSEQLIREALKNSL"
     gene            complement(4759..5382)
                     /locus_tag="PXA33_001768"
     CDS             complement(4759..5382)
                     /locus_tag="PXA33_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HDL5121080.1"
                     /translation="MLTILFIHFAGLISPGPDFFYVVRQSASHSVKSGVLAATGISIG
                     IIFWAAFAIFGLAWLSNSIGSLFQYAIMIIGGCYLVYIGSQMVRVTENIIFVENKAQL
                     TPLNPWQEIKKGLLINIFNAKAGVYFTSVISAYVDNLSNTLQMFELLLLFVVSTFIYF
                     CVVALLFSRRPVRLFYAKYSRYIDNISGVIFILFGSMLIYEGFSHFI"
     gene            complement(5389..5862)
                     /locus_tag="PXA33_001769"
     CDS             complement(5389..5862)
                     /locus_tag="PXA33_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HDL5121081.1"
                     /translation="MKPINLKNPIHFFAFGFGSGLLKPAPGTWGSLAGLIVAIALWSI
                     TQSALFFGLLTLVSFIAGCVICTKASQDLEVHDDGRIVWDEIVAIFLMFTCLPEYNLL
                     GYLLAFISFRIFDILKPFPIRYFDEKLESGLGIMFDDILAAIFALISLHLLYYFI"
     gene            complement(5867..6847)
                     /gene="thiL"
                     /locus_tag="PXA33_001770"
     CDS             complement(5867..6847)
                     /gene="thiL"
                     /locus_tag="PXA33_001770"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HDL5121082.1"
                     /translation="MGEFDIIERYFNASQRPPRKDVLLSIGDDCALTSLKPNQQLAIT
                     TDTLVCGTHFLPTISPADLAYKSVASNLSDLAAMGAEPAWISLALTLPEINHDWLAEF
                     SEHFFEILDHYNVDLIGGDTTCGPLSLTITAQGIVPQDRGLFRHNAKVGDWIYVSGTL
                     GDSAGGLQLLLNQQSEQVNWNKEQSYLIQRHLRPTPRVLLGLTIASIANAAIDISDGL
                     LSDLSHILKRSDCGAVLNLENFPLSDVLLSCYSRTEAEYFALNGGEDYELCFTVPNDN
                     KAKLDKALANIGVTYTCIGQIRSAKEGLTLLRHNQPVTLPSQRGYDHFKK"
     gene            complement(6910..7323)
                     /gene="nusB"
                     /locus_tag="PXA33_001771"
     CDS             complement(6910..7323)
                     /gene="nusB"
                     /locus_tag="PXA33_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HDL5121083.1"
                     /translation="MKISPRRRARECAVQAIYSWYISQNTVEEVELAFVTDQDMKGVD
                     MPYFRKLFRGVVDNVEAIDEALRPYLDRKESDVDPIERSILRLSTYELKFEHDVPFRV
                     VINEGIEVAKVFGSDDSHKYINGILDKLAPALGRK"
     gene            complement(7404..8717)
                     /gene="pilQ"
                     /locus_tag="PXA33_001772"
     CDS             complement(7404..8717)
                     /gene="pilQ"
                     /locus_tag="PXA33_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HDL5121084.1"
                     /translation="MWRIFIYFLCGFSLAYANTISIVLKNAPTSLIFTYLAEETGKNM
                     VLDDNIETKSTLRLENKSVDEIFKTLGKVNKLSLTQEDDIVYIHKKEEKAADLTPLPI
                     MNLQNNGQNTPLVTAPKLITKTLKLHYAKASEVIESLTKGSGTFLSENGYIHFDERSN
                     SLIVKDSAKSLKNIENLVKQLDQPTEQIAIEARIVTISSEHLQELGVRWGIFSRGAGH
                     YKFGGRLEGNGLNNVTNNLNVNFPITGGASAVLQVSSINSRVLDLELSALEQENSVEI
                     IASPRLLTTNKKPASIKQGTEIPYVMYNTKSEATDVEFKEAVLGLEVVPHLSTQNQIL
                     LDLIVTQNSPNSQSGSSGLITIDKQELNTQVFAKHGETIVLGGIFQHLTQKGEDKVPI
                     LGSIPFIKRLFSQTRDKISKRELVIFVTPYILQSNEKSSNRKKSK"
BASE COUNT         2568 a         1702 c         1869 g         2635 t
ORIGIN      
        1 ctcaaaccaa gccttatttg acattaactt agagattgaa aaaggggata ctgttgtatt
       61 gcttggcccg agtggggcag gtaaaagtac cctaattcga accttaaact taatggaagt
      121 accgcaatca ggcactttgg cgattgcaaa tcatcaattt gatttaactg ctaaaaccga
      181 cagcaaagcg gtgagtttgt tacgccgtga ggtgggaatg gtgttccagc agtatcattt
      241 atggccgcat ttaaccgtaa tgcaaaactt aattgaagcc ccgatgaaag tgcttgggtt
      301 aagcaaagaa gaagcggttg ccagagcaaa aggacatttg gcacgtttgc gtttgagcga
      361 gtttgccgag cgtttcccac tgcatctttc aggtggtcag caacaacggg tggcgattgc
      421 cagagcactg atgatgcagc cacaggtctt attatttgat gaacctaccg cagcacttga
      481 cccggaaatt actgcacaag tagttgatat tattaaagaa ttgcaatcta caggcattac
      541 tcaggtgatt gtgactcacg aagtaggcgt tgctcgtaag gtggcaagca aagtggtgta
      601 catggaaaaa ggtacaatta ttgaacaagg tgatgcaagt tgttttgaat caccgaaaac
      661 agagcaattt gctcaatatt tatctcactc agagtaatct ataacaacaa aaggacacaa
      721 caatgaaaaa attattatta gcttccgcta tcgcatttgc aacgctttct gccaatgcac
      781 aagaaactat cacttttgcg atggagcctt cttacgcccc ttttgaaaca acaaatgaaa
      841 aaggtgaagt tattggcttt gatgtagata ttgcaaaagc attatgtgaa gaactaaaag
      901 caacttgcga atttaaaacg caatcattcg atagcttaat tgcgggatta aaatttaaaa
      961 attttgatgc aattatttca ggcatggata ttactgaagc gcgtcaaaaa caggttgcct
     1021 ttaccaatgc ttatttagaa aactcagtaa gttatgtcgc aatgaagggg aaatttacac
     1081 cggaaacagc taagaatgtc ggcgtgcaaa atggtacaac ttttcaacaa tacttaagca
     1141 aaaatgccaa acaatataat ttaaaacctt acgctagcct acaatcagca gtgttagatt
     1201 taaaaaatgg acgtgttgat atggtatttg gtgatacacc agtattggca gagtggctaa
     1261 aaaccgaaaa agagttagat ttcgtgggtg aaaaagtagt cgataaagcc ttttttggta
     1321 atggatatgg tattgcggta aataaatcta acactgcatt gcttgaaaaa ttaaattcgg
     1381 ctttagctac catcaaaaca aatggtaaat atgagcaaat ttataatact tggatggctg
     1441 ggaaataatg tctagcgata ttttaaactt acttgtcaac gcaaccctga tgaccttagg
     1501 gattgcgctg gcaagcctct tgattggaat gttattgtca atcttttttg tggtacttga
     1561 aaccagtaag attgcatgta tccgtaaacc aactagtata ataattacat tattgcgtgg
     1621 gttgccggag attttagtgg tatttttgat ctattttggt cttatccaag ttattttctt
     1681 gatcaccgat gagtttgcgg agttaaatgc tttctggtgt ggggctattg cgctgggtat
     1741 tatttttgct gcttatgctt cccaatcttt acgtggtgca attaaagcta ttccagttgg
     1801 gcagtgggaa tctggtacgg cgttagggtt aagccgagcg caagtatttg ttcgcattat
     1861 tctccctcaa gtctggcgac acgctttacc aggcttaagc aatcagtggt tagtgctgtt
     1921 aaaagatacg gcattggtat cgttaattgg ggtagatgat ttaatgcgtc aatcggagtt
     1981 aattaatgct aatactcatc aaccttttac ttggtatggg atagcagcac ttatttattt
     2041 agcgattact ctaattagcc agcgagtgat taaagtatta gaaggtagat ttacccgttt
     2101 tgagcgagga gttgcttaat gtggtgggat tatttagttg aaatttcaca gggtattggc
     2161 accagcctac agctcacttt tgtggcgctc atcattgcct ttattttagc gatcattctt
     2221 acgtttattc tctcgttaga aaatcgtccg ttaaagtggt tggtaaacgg atttattacg
     2281 ctttttaccg gaacgccttt attaattcaa tttttcttga tttatagtgg cccggggcaa
     2341 tttgaatgga ttacagaaag tgcggcttgg gtattattat ccgacgcttg gttctgtgca
     2401 atgttggctt tagcgttaaa cagtgctgcc tattcgacat tgttgttcca cggtgcggta
     2461 aaagcgattc cgaaggggca atgggaaact tgcgcagccc ttggtttgag ccgtttagat
     2521 acactgaaaa ttttagtgcc ttacgcatta aaacgtgctt taccttctta tagtaatgag
     2581 attgtgctag tttttaaagg cacatcgctt gcttcaacca ttacgattat ggatattatg
     2641 ggctatgctc gccagcttta tggtacagag tatgacgcat tatcaattta tggtatggca
     2701 ggggttattt acttggtaat taccggcatt atgacaatcg ttctcagaaa attagaaagc
     2761 agagtattag cttttgagcg ttttgagact gaaaaagcct agcggataag cggtcaaatt
     2821 ttattgattt tttgcaaatg aaagcggata gtactaacta tccgcttttt atttatgtat
     2881 caatgccttt agaaatatga ctaatctatt tgggttatac ctaaatgggc ataggttctg
     2941 ctggtagcaa ttctgcctct tggtgtgcgt tgtagaaagc cttgctgaat cagataaggc
     3001 tctagcacat cttcaatcgt atctcgttct tcgccgatag ctgccgcaag attgtctaat
     3061 ccaactggcc cgccatcaaa gcgttctaca atcgcttgca acagtttaat gtccataaaa
     3121 tcaaagcctt ccgaatccac atcgagcatt gccagagctt gtttggcaat gtctgaggta
     3181 ataatgccat tattacgcac atcggcgtaa tcccgaactc ggcggagtaa gcggttggca
     3241 atacggggtg tgccacggga gcggcgagcc acttcataag caccgtctgc cgacaaattg
     3301 agatttaagc aatcggcgct gcgtttgaca atcagagtaa gatcgtccac tgagtaaaat
     3361 tctaagcgtt gtacaatccc aaaacgatct cgtaacggtg aggtcagtga gccggcacgg
     3421 gtggttgccc ctacaagggt aaaaggcggt agatcgagct tgatggaacg agcagcaggt
     3481 ccttccccaa tcataatatc gagctggtaa tcttccattg caggataaag cacttcttca
     3541 atcgctggag aaaggcggtg aatctcatca ataaacagca catcgtaagg ttcaaggttg
     3601 gtcagcattg ctgccaaatc gcctgctttt tccagcaccg gccctgaagt tgtgcgaata
     3661 ttcaccccca tttcgtttgc cacaatgttg gcaagggtag ttttccccaa accgggcggg
     3721 ccgaaaatga gcaagtgatc taatgcctct tcccgcaatt tagcggcttt gatgaagatc
     3781 tccatttgat catgcacaga aggctgcccg acatagtcgg caagcagttt cgggcggatt
     3841 gctcggtcta taacttcttc ctcacgctta ggcgaggcac tgatgattct atctgcttca
     3901 atcatacatt tagattattg ttgtagtgga atggtattac cctatattac tcatgagggc
     3961 aaatattgat ttgcccctac acatatattt ttaaaaatga gattgtaggg gcgaaatatc
     4021 tttcgcccta tactatcaat atcataatga attttttaac gcctcacgaa taagctgttc
     4081 gctcgttgca ccggatttat tcactttctt aatcattttt tccgcatcgg caggtttgta
     4141 gcctagggct attaaggcat caagggcttc atttgccggg tctggcgtat tacttgccac
     4201 aatttgctcg tgcgatactt ccacaaagaa atcactctgt gccattcctt taaatttgcc
     4261 tttgagttcc accaataagc gttctgcggt tttccgaccg attcccggaa ttttggttaa
     4321 ttttgccagc tcttcatttt caatcgcggt ggcaaattgc gacaccgaca ttgctgatag
     4381 aattgccaac gcgagcttcg gcccgacacc gttggtttta attaattcac ggaatagagt
     4441 tctatcttgt ttttgggcaa agccgaatag caagtgagca tcttctcgca ccactaaatg
     4501 ggtgaaaata gtcgcctctt cgccaatttg tggcaggctg tagaaactgg tcatcgggag
     4561 cagtagttca tagcctaccc cttgcacgtc taataaaatt tcaggcggtt gcttttcaat
     4621 aattttgccg tgtaatcgtc caatcatttt gttctctcag agttaaaatt ggcagtagca
     4681 tagcagaaac tgtataaaat aacactattt attgatagcg gaatttgcaa atttttagtg
     4741 aaatcagacc gcttgtgatt agataaagtg gctgaatcct tcataaatca gcatcgagcc
     4801 gaataagatg aaaattacgc ctgagatatt gtcaatatag cgactatatt tggcatagaa
     4861 cagtctgacc ggtcggcgag aaaagagtag tgccaccaca cagaaataaa taaaggtact
     4921 taccacaaag agtagaagta gttcgaacat ttgtagggta ttgcttaaat tatctacata
     4981 ggctgaaatg acgctggtaa agtaaacgcc agctttggca ttaaagatat tgattaataa
     5041 gcctttttta atttcctgcc aagggtttag cggcgtgagt tgagctttat tttcaacaaa
     5101 aatgatattt tcggttaccc gtaccatttg agagccaata taaaccaggt aacagccacc
     5161 gataatcata atggcatatt gaaacagcga gccaatacta ttactgagcc acgctaaacc
     5221 aaaaatcgca aaggctgccc aaaaaataat gccgattgag attccggttg cggcaagtac
     5281 cccactttta actgagtggc tggcggattg tctgacgaca tagaagaaat cggggccggg
     5341 gctgataagc ccggcaaaat gaataaataa aatagtgagc ataaattttt aaatgaaata
     5401 atataaaaga tgaagtgaaa tcagggcaaa aatggctgcg agaatatcat caaacataat
     5461 gcctaaaccg ctttctaatt tttcatcaaa atagcggatt ggaaaaggct tcaaaatatc
     5521 aaaaatgcga aagctgataa aggcgagcag gtagccaaga agattgtatt caggcaaaca
     5581 ggtgaacatc aaaaagatgg caactatttc gtcccaaaca attcgcccgt catcgtgtac
     5641 ttctagatct tggctggctt tggtgcaaat aacacaaccg gcaataaaag aaactagggt
     5701 gagtaagccg aaaaacaggg cggattgtgt gatagaccac aaggctattg ctacaattag
     5761 cccggcgagg ctaccccagg tgccgggagc aggctttaat aagcctgaac caaagccaaa
     5821 ggcaaaaaaa tgaatcggat ttttaagatt aattggtttc attgcgttat tttttaaaat
     5881 gatcgtagcc tcgttgtgag ggcaaggtga caggctgatt atgacgtaat agggttaaac
     5941 cttctttagc cgaacgaatt tgcccaatac atgtataagt tactccgata ttggctaatg
     6001 ctttgtctaa tttggctttg ttgtcatttg gcacggtaaa gcagagttcg taatcttcgc
     6061 caccgtttaa ggcaaaatat tctgcctctg ttcgagaata acaactaagt aagacgtcag
     6121 acaaggggaa attctcaagg tttaatactg caccacagtc actgcgcttt aaaatatggc
     6181 ttaaatcaga caatagacca tctgaaatat caatcgctgc attagcaata gaggcgatag
     6241 tcaagccaag taaaactcga ggtgttgggc gtaaatggcg ttgaatcagg tagctttgct
     6301 ctttattcca attcacttgc tcgctttgct gatttagcag aagttgcaaa ccacctgcac
     6361 tgtcaccgag tgtgccggaa acatagatcc aatcgcctac tttagcatta tggcggaata
     6421 agcctctatc ttgtggcaca atcccttgag ctgtgattgt caaagatagt ggtccacaag
     6481 tagtatcccc tccaatcaaa tcaacattat agtgatcaag aatctcaaag aaatgttcac
     6541 taaattcagc tagccaatcg tgattaattt caggtaaggt taaggctaaa gagatccatg
     6601 caggttctgc acccattgcg gcaagatcgc ttaaattgga cgccacactt ttataagcga
     6661 gatcggcagg tgaaatagtc ggtagaaaat gagtgccgca aacaagggta tcggttgtga
     6721 tcgcaagttg ctgatttggt ttgagtgatg tgagcgcaca gtcatcgccg atagaaagta
     6781 aaacatcttt gcgaggtggg cgttgagaag cattaaaata gcgttcgata atatcaaatt
     6841 cacccataaa aaatctcctt gagcgttata atcaaaatcc ccaatttatg tataaattgg
     6901 ggaatcttat tattttctgc ctaatgctgg ggctaattta tctaaaatgc cgttaatgta
     6961 tttgtggctg tcatcagagc cgaacacttt agcgacttca atcccttcat tgatgactac
     7021 acggaaaggc acatcgtgtt caaattttaa ttcgtaagtt gataaacgca aaatagaacg
     7081 ttcaatagga tcaacatctg actctttacg atctaaataa gggcgtaatg cttcatcaat
     7141 ggcttctaca ttatccacaa cgccacggaa aagtttacgg aaataaggca tatcaacgcc
     7201 tttcatatct tgatcggtca caaacgccaa ttccacttct tcaaccgtgt tttgggaaat
     7261 ataccaagaa taaatggctt gcaccgcaca ttctcttgca cggcgacgtg gggaaatttt
     7321 cataaaacct ctaaatcttt aactcaaaaa atgacggctg attctatcac acttccccta
     7381 tttttgcttg cgtttatgat gtgctatttt gattttttgc ggttgctgct cttttcatta
     7441 gattgaagga tataaggcgt cacaaaaatg actagctctc ttttgctgat tttatctcga
     7501 gtttgactaa ataaccgctt gatgaaggga attgagccta aaatcggcac tttatcttcg
     7561 cctttttggg ttaaatgttg aaaaatacca ccaagtacaa tggtttcgcc gtgtttggca
     7621 aatacttgcg tatttagctc ttgtttatca atcgtaatta agccactact gccggattgg
     7681 gaatttggcg aattttgggt aacgatcaga tcgagtagaa tttgattttg ggttgaaagg
     7741 tgtggaacga cttcaagccc taataccgct tctttaaatt cgacatcggt ggcttccgat
     7801 ttggtgttgt acatcacata aggaatttcc gtgccttgct tgatacttgc cggcttttta
     7861 ttggtggtta acaggcgtgg gctggcaata atttccacac tattttcttg ttctaatgca
     7921 cttaattcta aatcaagcac tctgctatta atggacgaaa cctgtaatac ggcagatgct
     7981 ccaccagtaa tcggaaaatt tacatttaag ttattggtga cattgttgag tccattgcct
     8041 tccagccgtc cgccaaattt atagtgccca gccccacgag aaaaaattcc ccagcgaacg
     8101 ccaagctctt gcaaatgctc gctactgata gtgacaattc tagcctcaat cgcaatttgc
     8161 tcggtgggtt ggtcgagctg ttttactaaa ttctcgatat ttttaagcga ttttgcactg
     8221 tctttcacaa tcaggctatt gcttctttca tcaaagtgaa tataaccgtt ttccgataaa
     8281 aacgttccgc tgcctttggt taacgactca atcacctctg aagcctttgc ataatgtagt
     8341 ttcaaggttt ttgtgattaa cttaggggcg gtgacaagcg gtgtattttg cccattattt
     8401 tgcaaattca ttattggcag aggcgttaag tcagccgctt tttcctcttt tttgtgaata
     8461 taaacaatat catcttcttg cgttaaggat aatttattga ctttgccaag cgttttaaaa
     8521 atctcgtcaa ccgatttatt ttctaatcgt agtgttgatt tggtttcaat attatcatca
     8581 agcaccatat ttttaccggt ttcttccgct aaataagtaa aaataagcga agttggagca
     8641 ttttttaaaa ctatggaaat ggtattcgca taggctaatg aaaaaccgca taagaaatat
     8701 ataaaaattc gccacatatc aacctctata atttgatgtt aatttcataa gggatattac
     8761 aatctttagt taat
//
LOCUS       DANLRK010000106         8770 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.106, whole genome shotgun sequence.
ACCESSION   DANLRK010000106 DANLRK010000000
VERSION     DANLRK010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8770
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.106"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(24..2183)
                     /locus_tag="PXA33_001773"
     CDS             complement(24..2183)
                     /locus_tag="PXA33_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent
                     hemoglobin/transferrin/lactoferrin family receptor"
                     /protein_id="HDL5121085.1"
                     /translation="MQIKQTVLAIAITPFISQLSLAQETKISSIELDTIEVNEQQENL
                     LKQQAVMSNEDLVRYSTDMGVPQTGRFGPQGFNIRGVEGNQVAVTVDGISQAASNNYA
                     TFSRYSYYNKSRPEIDTELMSDVTIEKGSSTKVGNGALGGSVNYKTKTVSDILMKGRQ
                     FGGMLKYTYNGQNKENIETAGIAADGQYADALVMYSHRQGHETQTTSKGPDIMGSGRG
                     LPNPYKNNQYSYLAKLGLKWNDENRLELKTSKQRSKNAGQELSYSTVTLRDFLDVQEI
                     ETYGVTYLYTPKNSILENIKVAVTKQDTEISGINDQITIARGTKTDNTKRAFNTKNVE
                     GSLGVTFKPVDLGNTEHKLSTELGYGVSDFSFDMQTYTAGITPTDQNMQSPAKTKSFH
                     ILMGDSILLTQNLSAHLNARYEQSKLHSDDNSLSDKTFNNWAGDIGLNYQLSPVWQIG
                     YKLSRGFRTPTASEMFFNREVQGRGASQTFLANTNLKPETSLGNEISLVGNGSLGSLS
                     VAAYHTHYRNLIDLATIGNNIYQSQNVNRAKIKGVDINGTLDLNTAWNAIPQGFELNG
                     GFGYARGKRNDGTDLLTVQPLKALFGIGYRAVNNDWAIHLNGTYTQGKKAKYAQQYAI
                     LANTGYLSYDKSLTTYPYLSNSATVFDLVAHKKFGERAIVKVGVYNLFNKKYWTWDSL
                     RTIGITSPGVANSITGEGLNRYLAPERYVMASVEFKF"
     gene            2290..3156
                     /locus_tag="PXA33_001774"
     CDS             2290..3156
                     /locus_tag="PXA33_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL5121086.1"
                     /translation="MEYKMRTLLFSFIALSMCLNTTKAAEKIVSTAGYASEIVAALGK
                     ADKLVGVDTTSVKPQTIMEKKPKIGYRRQLSAEGILSLTPDLIILAPDAGPNAVIEQI
                     KASGIPLLHLGEKPSLAGIREDIQQIAKAIGAESEAKQLTETILADEKKLADIRSQHS
                     TDKEVLVLIDTASQGIFGLGKKSVGDHFLTLLNLNNSFNAEGNKPLANEALAASNADV
                     ILLASRNQAKESATISKLAESSPQYAQLTNTQAGKKGCVFAINILDALGFGPYTAQYA
                     HQILTTIQPCLK"
     gene            3167..4153
                     /locus_tag="PXA33_001775"
     CDS             3167..4153
                     /locus_tag="PXA33_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDL5121087.1"
                     /translation="MKLHLTLIFTLIISLVICTHLGAMSLPLSAWHCNNAECESAAYI
                     LWQIRLPRLLGALFIGAALAISGATMQGLFRNPLVDPGIIGVSSGAALAAALYIVVIQ
                     PLLPESISYYALPIAAFLGGWLVTFMLYLLSQRNGQLHIAIMLLIGIALAAFSGALTG
                     ILIYVADDNQLRSLTFWSMGSLAGMNWQMVAMMAAVVFICSPLIWREHRALNALTLGE
                     EDAQYIGFDIAKVKRRLVFYVALLTGTSVACVGAIGFVGLVIPHLMRLLGGSNHRHLL
                     LNSLVCGAILLMIADTLARTLAAPAEIPIGIFTALFGAPFLAVMVWKSGKHI"
     gene            4153..4959
                     /locus_tag="PXA33_001776"
     CDS             4153..4959
                     /locus_tag="PXA33_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL5121088.1"
                     /translation="MSNLLTCQNLSIVHQQKKLLEIQEIAFEQGKFTAIIGPNGAGKT
                     TLLKALSGNNAQTNSAVFYQHQRLTDLNGYALAQKRAMLSQQSQIAFPLKVEELVQLG
                     REPYRQSEFAKFNEKITAWAMETMSLTALKERSSIQLSGGEQHRAHIARVLAQLVPEP
                     NADLSGSWLLLDEPTNHLDIHHQYQLFSLLQKLKQQGLTIIAILHDPALAINQADHIL
                     MLKNGEKFAEMSAKAIAESCLLDELYEMKMGCRYCPHSGQYYLCPALAES"
     gene            complement(4980..5618)
                     /gene="purN"
                     /locus_tag="PXA33_001777"
     CDS             complement(4980..5618)
                     /gene="purN"
                     /locus_tag="PXA33_001777"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005693938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HDL5121089.1"
                     /translation="MKKFVVLISGNGSNLQAMIDAQKSADTSGQICGVICNKADAYGL
                     IRAKQAGIPTFVFSRKDYQSNVEMDLAIAEQIEQLGAELIVLAGYMKILTPEFTQHFA
                     GKILNIHPSLLPKYPGLNTYQRAIEAGESEHGTTIHFVNEEVDAGAVVLQAKVPIYPE
                     DEIEDVMARVVEQEHRYYPLVIEWFCSGRLVSQHGKAYLDGKLLPACGYAED"
     gene            complement(5725..7882)
                     /locus_tag="PXA33_001778"
                     /pseudo
     CDS             complement(5725..7882)
                     /locus_tag="PXA33_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484758.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            complement(7936..8733)
                     /locus_tag="PXA33_001779"
     CDS             complement(7936..8733)
                     /locus_tag="PXA33_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HDL5121090.1"
                     /translation="MAFFLLGCSVSTQSLNEQNVDSIGQIIDLNTNQTISFTELLSRL
                     SSQDYVLVGEEHTQLIHHQIELYLFNQLTKKAKLHLIALEMLNVDQQPAIDRIQFNRP
                     VNLSATDLREAIQWQKWDWKMYQDLVVESLGSNSRVIATNLTDKEVEILLNGAEPLKG
                     SVSTSSEVKQKIAQLLLSMNHGMPYPMENMVAVQQFRDRRMAEKLVKNALSTSLLIAG
                     NHHVRKDLGVPLHIAEYDRTKKVAVLMLKTEREEITSSQADYLWVTQ"
BASE COUNT         2547 a         1860 c         1626 g         2737 t
ORIGIN      
        1 actcttacat ttaatgctta tctctaaaat ttaaactcca ctgatgccat tacatagcgc
       61 tctggagcaa ggtaacgatt taagccttcg ccagttatac tgttggcaac tccaggacta
      121 gttataccaa tagttcttaa actatcccaa gtccaatatt ttttattaaa gagattataa
      181 accccaactt taacaatcgc tctttctcca aactttttgt gtgcaactaa gtcaaatacg
      241 gtggctgaat tacttaagta tgggtaagtg gttaatgatt tatcgtaact taaataccca
      301 gtattggcta aaatagcata ctgttgtgca tattttgctt ttttgccttg tgtgtatgtg
      361 ccgtttaggt gtatcgccca gtcattgttc acagctcgat aacctatgcc gaacaatgct
      421 tttaacggtt gaaccgtgag taagtcggta ccatcattac gtttgccacg agcataacca
      481 aatccaccat taagctcaaa gccttgtgga attgcattcc acgctgtgtt taagtctaaa
      541 gttccgttaa tatcaacacc tttgatttta gctcggttaa cattttgcga ttggtaaata
      601 ttgttaccaa tagtggcaag atcaatcaga tttctatagt gggtatggta agcggctacg
      661 ctcaagctac ctaatgaacc attacctacc agtgaaattt cattacctaa gcttgtttcc
      721 ggttttaagt tagtatttgc taagaaagtt tggcttgctc cacgcccttg aacttcacgg
      781 ttaaagaaca tttcacttgc ggtaggtgtt ctaaaaccac ggctgagttt atagccaatt
      841 tgccatacag gagagagctg ataatttaaa ccaatatctc ccgcccaatt gttaaaggtt
      901 ttatctgaca aactattatc atcagaatgt aatttcgact gctcatatcg agcatttaaa
      961 tgagcagata gattttgtgt cagtaaaata gagtctccca tcaaaatatg gaaagacttg
     1021 gtttttgccg gagactgcat attttgatcg gttggcgtaa tacctgctgt ataggtttgc
     1081 atatcaaaac tgaaatcaga tacaccatag cctaattccg ttgacaattt atgttcggta
     1141 ttacctagat caacaggctt gaatgtaaca cctaagctac cttcaacatt tttagtatta
     1201 aaagcccgtt tggtattatc tgtttttgta ccacgggcaa tagtgatttg atcgttaata
     1261 cctgaaattt cagtatcctg tttggttacc gcaactttaa tattttcaag aatactgttt
     1321 tttggagtgt ataaataagt aacgccataa gtttcaattt cctgcacatc caaaaaatca
     1381 cgtaatgtga cggtagaata agaaagctct tgaccggcgt ttttactgcg ttgcttagaa
     1441 gtttttaatt caaggcgatt ttcatcattc cattttagcc ctaattttgc caagtagctg
     1501 tattgattat ttttgtaagg atttggtaaa ccacgaccac ttcccataat atccggacct
     1561 ttagacgttg tttgtgtttc gtgtccttga cggtggctat acataaccaa agcatccgca
     1621 tattgaccat ccgcagcaat acctgccgtc tcaatattct ctttattttg accgttatag
     1681 gtatatttca acatcccacc aaattggcga cctttcatta aaatatcact tacggtttta
     1741 gttttatagt tcacagagcc gcctaatgcc ccattgccta cttttgtgct tgagcctttt
     1801 tcaatggtta catcgctcat taattcagta tcgatttcag ggcgagattt attgtagtaa
     1861 ctatagcgac taaaggtggc atagttattt gatgctgctt gcgatatgcc atcaactgtt
     1921 actgccactt gattaccttc aacaccacgg atattaaacc cttgtgggcc aaagcggcct
     1981 gtttgcggaa cgcccatatc agtactataa cgtactaaat cttcattgct catcaccgct
     2041 tgctgtttaa gtaggttctc ctgctgctca tttacttcaa tagtatctag ttcaatgcta
     2101 ctaatctttg tttcttgagc taaggagagt tgagaaataa agggagtaat cgctattgct
     2161 aatacagttt gctttatttg catattttag atcctctagt tagtttaatc cgtttaatgt
     2221 taacactata tcattttaat tgaaaattga aataataatt attattattt aagtttagat
     2281 aaaaatcaaa tggagtataa aatgagaacc ttactttttt ctttcattgc cctatcaatg
     2341 tgtttaaata ccacaaaggc ggcagaaaaa attgtttcaa ctgcgggcta tgcttccgaa
     2401 attgtcgcag cactaggaaa agctgataag ttagtcggtg tggataccac ttcagttaaa
     2461 cctcaaacga taatggagaa aaaacctaaa atcggttatc gtcgccagct ttctgctgaa
     2521 ggtattctct ctttaacccc agatttgatt attttagccc ctgatgccgg ccctaacgca
     2581 gttattgagc aaattaaagc cagcggtatt ccattacttc acttaggaga gaaaccgagc
     2641 ttagcaggga ttcgtgagga tattcagcaa attgccaagg cgattggtgc agaatcagag
     2701 gcaaaacaat taacagaaac cattctggca gatgaaaaaa aattagccga cattcgtagc
     2761 caacattcta ctgacaaaga agtcttagtg ctgattgaca ctgcatcgca aggtatcttt
     2821 gggctaggga aaaagagtgt gggtgatcat ttcttaacct tgttaaatct aaacaatagt
     2881 ttcaatgctg agggcaataa gccactcgcc aatgaggctt tagctgcaag caatgccgat
     2941 gttattttat tagcctcccg taatcaggca aaagaatctg caacgatcag taaattagca
     3001 gaatccagcc cacaatacgc ccaactcaca aatacacaag cgggcaaaaa aggctgtgtt
     3061 tttgcaatca atattttaga tgccttgggc tttgggcctt acactgctca atatgcacac
     3121 caaattctca ccactattca accttgttta aaataacgga tacgctttga aactgcattt
     3181 aaccttaatt ttcactttaa ttatcagctt agtgatttgt acccatttag gggcgatgtc
     3241 attaccccta tccgcttggc actgcaacaa cgctgaatgc gaaagtgcag cctatatttt
     3301 atggcaaatc cgcctgcctc gattactagg ggcattattc atcggggcag ccctcgctat
     3361 tagcggagca acaatgcaag ggctgtttcg caatccgtta gtagatccgg ggattattgg
     3421 tgtctcttca ggtgctgcat tggctgcggc attatatatt gtggtgattc agccactact
     3481 gccggaatca atcagctatt atgctctacc tattgccgcc tttttagggg gctggctggt
     3541 gacttttatg ctctatttac tttctcaacg taacgggcaa ctacatatcg ccattatgct
     3601 attaattggt attgctctag ctgcatttag tggcgcttta accggcatac tgatttatgt
     3661 tgccgatgat aaccaacttc gtagtttaac cttttggagt atgggatctt tagcaggtat
     3721 gaattggcaa atggtggcaa tgatggctgc ggttgtcttc atttgtagcc cacttatttg
     3781 gcgggaacac cgtgcgttaa acgccttaac attaggtgaa gaagatgcac aatacattgg
     3841 ttttgacatt gcaaaagtca aacgtcgtct tgtgttttac gttgccttat taaccggcac
     3901 aagcgttgcc tgtgttgggg ctatcggctt tgtcgggctg gttattccac atttaatgcg
     3961 tttactggga ggcagtaacc accgccattt attactcaat agtttggttt gcggtgcaat
     4021 cttattaatg attgcagata ctcttgccag aaccctagcc gccccggcag aaatcccgat
     4081 tggtattttt accgccttat ttggtgcgcc attccttgca gtaatggtat ggaaaagtgg
     4141 gaaacatatc taatgagcaa tttattaact tgccaaaatt taagcatcgt tcatcaacaa
     4201 aaaaagttgc tggagattca agaaattgcc tttgaacaag gaaaatttac cgccatcatc
     4261 ggtccaaacg gagccggcaa aacaaccttg ctcaaagcct taagcggtaa taatgcacaa
     4321 actaacagtg ccgtatttta tcaacatcaa cgcttaacag acttaaacgg atatgctctg
     4381 gctcaaaaac gggcaatgct cagccaacag agccaaattg cctttccctt aaaagtagaa
     4441 gaattggtac aactcggaag agagccatat cgccaaagtg aatttgcaaa atttaatgaa
     4501 aaaataaccg cttgggctat ggaaacaatg tctttaaccg cattaaaaga aagaagtagc
     4561 atacaacttt ccggtggaga acaacatcga gcgcatattg ctcgagtgct ggctcaactc
     4621 gttcctgaac cgaatgccga tttaagcgga agctggcttt tgcttgatga acccaccaac
     4681 catttggata ttcatcacca atatcagctt ttttccttac tacaaaagct gaaacaacaa
     4741 ggcttaacta tcattgcgat tttgcacgat cctgcattgg caataaatca agccgatcat
     4801 atcttaatgc taaaaaatgg agaaaaattt gctgaaatgt ccgctaaagc cattgcagaa
     4861 agttgtttat tagatgagct gtatgaaatg aaaatgggct gtcgctattg ccctcacagc
     4921 gggcaatatt atctttgccc tgcattagct gaaagttagg gtgcgatgca ccctatcgtt
     4981 taatcttctg catatccaca agcaggcagt agtttaccgt ctaaatacgc tttgccgtgc
     5041 tgagaaacta atctacccga acagaaccac tcaatcacca acggataata tcgatgttct
     5101 tgctcaacca ctcgagccat tacatcttca atctcatctt caggataaat cggcacttta
     5161 gcttgtaata ccaccgcacc agcatccact tcttcattca cgaaatgaat agtcgtgccg
     5221 tgttcacttt ccccagcctc tatcgctctt tgataagtgt ttagccctgg gtatttaggt
     5281 aataaggaag ggtgaatatt gagtattttg ccagcaaaat gttgcgtaaa ttcaggcgtt
     5341 aaaattttca tatagccggc taatacaatt aactcagctc ctaactgctc aatttgttcg
     5401 gcaatcgcaa gatccatttc cacattgctt tggtaatctt tacgagaaaa tacaaatgtc
     5461 gggattcccg cctgtttagc tcgaatcagc ccataggcat ctgccttatt gcaaattaca
     5521 ccgcaaattt gaccgcttgt atccgcagat ttttgcgcat caatcattgc ttgtaggtta
     5581 gaaccattcc ctgaaattaa aaccacaaat tttttcatat tacacaccgg ctgaatattc
     5641 atcgttctgg atattatgcc ttgaatctca acatcagtac aggaaagaaa aagggcgtat
     5701 tggttaaaat aagccacttt caagctagaa acgcacctct gcccctagtg tgtaagttcg
     5761 acctctggca ctatttgtga aacgataggt gtctgtatca tagtcatact cttctgcggt
     5821 ggaagagtta tacgcattta aggcatcaag ataatttcgg ttcatcacat tttgtacccc
     5881 ggcttttaag gttaaatgtt tattcacttt gtaatttgca tataaatcga taatcaccgg
     5941 aatattcggt aactgctcgt gaataatgtt gccatcttcg tctaaatcaa taccgacacg
     6001 tttcgctttg cctgtatatt taaaaatgct agacagtgtt aattttttat ccagcaattt
     6061 agcccctagc tccaaagtcg cataatcttt tggtagctgg gtataagctt cagtgccaaa
     6121 tccataaaga atagtcgcac cttttgaagt gggtgctgtg ctttcagttc gggtataact
     6181 accattgaca aaaaaagcat cggaatcgta agccagctca acttcccaac ctcgattttt
     6241 aaccttttca gggtgattca cataaatttg gaactgtata gcattggtag catcacttga
     6301 atttcgacat actgttggcc cacaaccata caacgagagg ttatacaaat agtctttaat
     6361 gcgtgagtta aaatataccg cttttagccc caagatatca ttattgagta atacattatc
     6421 ttgatgaata ttaaacccaa tctcataggt tttcgcactt tctggtttca aaaacgggtt
     6481 cacactcaag ccatttcgac tggtattaaa catttcttgc acattaggcg aacgggaggt
     6541 ttgtttgtaa ctgacaaaag gttgaaacca atcagcaacc tgaaatgcta aagtggcaga
     6601 aggattcaac ttataatgtt ttttattaac atcaaaccca aaagaaggga aacaataatc
     6661 catatcatcg ctatcgcatt ctggcttata tccttttaac tgatagcgtg aatagtgaag
     6721 acttgaattt aaagtgacaa tgccgtattt ccattcggtg tctaggtaaa gggaatcaat
     6781 attttgctta cctgccggcc caaaagcggc attgctaatc gctccatcat tattcttact
     6841 ttccggtaaa tcacgggaat atttatttcc ctgactcaaa acggctggtg ttattcaaat
     6901 caaacatcca cattttgttt ttggttgagg catcttctaa tgtccatagt tttgcatcat
     6961 cgtggaaatc ttgcttagtt tctgttactg ctgttaggaa tttcagatca atccaatcag
     7021 attgaggctg gtaactggat tctaaggaat aggtcacact atcaatatta cgccccacta
     7081 agctattttt atattgacgt ccggacaatt taaactctga atcttgattt ggcttaaagg
     7141 caatttttcc catataagat tcagggtgat tttttaaccc ttgcatataa gcagaatccg
     7201 ttgccattgt gccattacct cggcgataat tttgactgat actacttttg ctaaaagcca
     7261 ataaactgcc aagagaacca ctttccagca catcaacttt tccggctagt gcaagcattc
     7321 cgttttttcc cagatcatta ttgccataat taaaacggct taatacgccg actttatgtt
     7381 gttctagtag aatatcttcg acatcaatcg ttttaaaatt agcactaccg gctaaagcat
     7441 tcaatccggc tgcaccattg ttgtagcctt tcgcaatatc aactcgggtt aaaaaatttt
     7501 gatcaattaa agtgctgaat tggctcattg gcccgttacc atcagtgtgg aacttaccac
     7561 ctccgtttgc tgatgtacca agctgtgttt gtggcacgcc atcaactaag gtatttaccc
     7621 gaccaagacc actcatatta cgaatattca cactcacgcc cccttgcccc gggtcaattt
     7681 gggtatagcc acctgcgaca gaacggacaa tactatctaa actttgcatt gcattcacat
     7741 tcattgctcg agaactcacc gagccggctt tactgaaatt ctcaaaatta gggttttcca
     7801 cagtgccata aaccgtcaca gtatcaagct ccgcaatatt tttcgccttt tcctctgcca
     7861 atgcagtttg cgatcctccc atacacaaaa ccggaaacaa aatgcgtgtg attatattaa
     7921 atttcataat tctccttatt gagtgaccca aagataatct gcctgtgaag aagtaatttc
     7981 ttctctctct gttttaagca ttagcaccgc tacttttttt gttcggtcat attccgcaat
     8041 atgcaacgga acgccaagat ctttgcgaac gtggtggtta cctgcaatca gcaagctagt
     8101 cgataacgca ttttttacta atttttctgc cattctgcga tcacggaatt gttgtactgc
     8161 caccatattt tccatcgggt aaggcattcc gtgattcatt gacagcaaca gttgtgcgat
     8221 cttttgctta acttcagagg aagtggatac agaacctttt aatggctccg ccccatttaa
     8281 taaaatttca acctctttat ctgttaagtt agtggctata acacgactat ttgaacctaa
     8341 gctctctacc actaaatctt gatacatctt ccaatcccat ttttgccatt gaattgcctc
     8401 tcttaaatcc gtggctgata aattaaccgg tcggttaaac tgaatgcgat caatcgcagg
     8461 ctgctgatcc acatttaaca tctccaacgc aattaaatgt aacttggctt tcttcgtaag
     8521 ttgattaaat aaatacaatt caatttggtg gtgaattaac tgagtatgct cctcaccaac
     8581 taaaacgtaa tcttgagaag atagacggga aagtagttca gtaaatgaaa tcgtttgatt
     8641 ggtattcaga tcaataattt ggccgataga gtccacgttt tgttcattaa gggattgcgt
     8701 tgaaaccgag cagcctaaaa gaaaaaaagc cataaaaagg ctagctaaaa aagaggaaat
     8761 acgaggtaac
//
LOCUS       DANLRK010000107         8700 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.107, whole genome shotgun sequence.
ACCESSION   DANLRK010000107 DANLRK010000000
VERSION     DANLRK010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8700
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.107"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            33..587
                     /gene="srlA"
                     /locus_tag="PXA33_001780"
     CDS             33..587
                     /gene="srlA"
                     /locus_tag="PXA33_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005725059.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HDL5121091.1"
                     /translation="MIEAITHGAEWFIGLFQKGGETLVGMINGILPLLISLLVVMNAL
                     IKFVGQDRIERLAQRSAGNPISRYLILPVIGTFVFCNPMTLSLGRFLPERYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLGLPLGPLAISYLLVGMFTNFFRGWI
                     TDFTTAFFEKRMGITLDREVHLSH"
     gene            618..1619
                     /locus_tag="PXA33_001781"
     CDS             618..1619
                     /locus_tag="PXA33_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HDL5121092.1"
                     /translation="MSNKAIYIEKGNGGWGGPLTLPVVEGKKVLYMTGGTRPAIVNKL
                     VELTGWEAVDGFKEGEPPAEEIGIAIIDCGGVLRCGLYPKRRIPTINIHSTGKSGPLA
                     EFIVEDIYVSAVKENNIRLIDADGSVPVATEQAKPTSSTTATAKEYDSSKKITEQSDG
                     LLAKIGMGMGSVAALFFQAGRDTIDTVLKTILPFMAFVSALIGIIIASGLGDVIAHGL
                     TPLANSPIGLVTLALICSFPLLSPFLGPGAVIAQVIGVLVGTQIGLGNIPPHLALPAL
                     FAINAQAACDFIPVGLSMAEAKQDTVRVGVPSVLVSRFLTGAPTVLIAWAVSAFIYQ"
     gene            1664..2062
                     /gene="srlB"
                     /locus_tag="PXA33_001782"
                     /gene_synonym="gutB"
     CDS             1664..2062
                     /gene="srlB"
                     /locus_tag="PXA33_001782"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDL5121093.1"
                     /translation="MHRLSKRENIYMTIIYQTTFTKIGNFAQESLTDDMLITFKQGAP
                     ADLQDYCFIHNPSELSSPLEVGDIAEFDGVAYPITAVGSVASENLSALGHITFRFDGA
                     NDAEFPGSVHVIGTPPQGLTENSTLIIKRD"
     gene            2110..2898
                     /gene="srlD"
                     /locus_tag="PXA33_001783"
     CDS             2110..2898
                     /gene="srlD"
                     /locus_tag="PXA33_001783"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HDL5121094.1"
                     /translation="MNNEKKVAIVVGGGQTLGAFLSEGLADAGYRVVVADLNGENAQK
                     VAADINAKQGEGNAIGVQVDAADEPSVEALAKATDTVFGRADVLVYSAGTAKASPIWD
                     FNLNDFDLSVKVNLTGYFLTAKHFSRLMIRDGIKGRIIQINSKSGKVGSKYNSGYSAA
                     KFGGVGLTQSLALDLADKGITVHSLMLGNLLKSPMFQSLIPQYAAKLGIPESEVEQVY
                     IDKVPLKRGCDYQDVLNVLKFYAGEEAAYCTGQSINITGGQVMF"
     gene            3067..3447
                     /gene="gutM"
                     /locus_tag="PXA33_001784"
     CDS             3067..3447
                     /gene="gutM"
                     /locus_tag="PXA33_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HDL5121095.1"
                     /translation="MDSITLLIILAMIMWLLQAVLGWLQIKAFNQAFMEISKKGKVTV
                     GRNSGRFNPKSVIVLAFDDEKKVVDSLCMKGFSVFARPQKLASVIGLSLDQIVPEKIF
                     PSDKRSQFALKVAISSGYSCSDQK"
     gene            3510..4157
                     /gene="hxpB"
                     /locus_tag="PXA33_001785"
     CDS             3510..4157
                     /gene="hxpB"
                     /locus_tag="PXA33_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HDL5121096.1"
                     /translation="MQINSVIFDMDGVMIDSEPQWAEAQIAVLADLGINITEQDCEQL
                     TRGKRIDELSDTWIKKFQLSVSNQALSEAILAHGCAAIEQHGKALTGLYELLDFLKSN
                     QIKLAVATSSPPVVIKAVFDRLKLWDYFPIRCSAMDEEYGKPHPAVYLSAVKKLGIEK
                     QECIVIEDSVTGLIAAKAANLTTFLVNTDYQNEKFSIADKRMASLLDVIEKLKQH"
     gene            4200..4997
                     /locus_tag="PXA33_001786"
     CDS             4200..4997
                     /locus_tag="PXA33_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005725056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor"
                     /protein_id="HDL5121097.1"
                     /translation="MKPFERRQQILAYLAQHGRTEVDVLADYFKLTGATIRKDLTSLE
                     EDKKVLRTYGSVVLIDEEILEREEKINKEDKDDKPLAQKTGINLQQKQAIGQKAAELI
                     NEGNSIIFDAGSTVLQMIPHLKAFDNLSLMTNSLTIFNEILKLNKSYNLLISGGTFRE
                     KSASFHGYFAESAFIGSNFDILFIGTDGLDLDVGLTTFNEVYKVSSLMCDAANKIVLL
                     ADSSKFGRKSPNIVCGLEKIHTIITDEGLSQEMREQIKAKGIELLVV"
     gene            5008..5943
                     /locus_tag="PXA33_001787"
     CDS             5008..5943
                     /locus_tag="PXA33_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HDL5121098.1"
                     /translation="MNHLDSAKETLNLYAQQIITLNHRLSEEFNEVVNMILACQGRVV
                     VGGIGKSGLVGKKMVATFASTGTPSFFLHPTEAFHGDLGMLKPIDMVILISNSGETDD
                     VNKLIPSLKNFGNKIIAMTGNPYSTLGRNADVILNIGVEREACLNNLAPTSSTLVTMA
                     LGDALAIALMKARDFRPEDFARYHPGGSLGRKLLNRVRDVMVRKVPTASLDTTFTECL
                     SVMNEGRMGVAVIMQDDKLEGIITDGDIRRTLAKFGAESLNKTADEIMTRNPKTINDT
                     EFLAKAEDLMKELKIHSLIAVNDEGKVTGLMEFSS"
     gene            complement(5987..6537)
                     /locus_tag="PXA33_001788"
                     /pseudo
     CDS             complement(5987..6537)
                     /locus_tag="PXA33_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005752768.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            6839..8698
                     /locus_tag="PXA33_001789"
     CDS             6839..8698
                     /locus_tag="PXA33_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HDL5121099.1"
                     /translation="MSNLKVKVQGFGRFLSNMVMPNIGAFIAWGFITALFIPTGWLPN
                     ETFAKLVGPMITYLLPLLIGYSGGKLVGGERGAVVGAITTMGIIVGSEIPMFLGAMIV
                     GPLGGWAIKTFDKAIDGKVKSGFEMLVNNFSVGIIGMILAMLSFAFIGGIVTQISAAL
                     GAGVGALVEAGLLPVTSIIVEPAKILFLNNAINHGVFTPLGTAQAQEVGKSILFLIEA
                     NPGPGFGILLAYMFFGRGTAKQTAGSASIIHFLGGIHEIYFPYVLMSPRLILAVIAGG
                     ATGVLTNSLLGGGLIAPASPGSIFAILAMTAPGAHFAVVASVILSCAVTFAIASILLK
                     TQKGEASLEDAQAQTQAMKAESKGVVNGNINKIYVACDAGMGSSAMGASMLRKKVQAA
                     GLNIEVQNLAINDLPQDAQLVITHKDLTARATQRVATAQHLSLTNFLDSAFYDSLVAK
                     LANHQPQQAVQNTQNFANPVEEPQAEGLKLQAAQVFLGLQANSKEEAIRFAGEQLVKA
                     GFVQPSYVDAMFAREQMVSTYLGEGLAVPHGTSDAKDSVLKTGIVVCQYPNGVRFTDE
                     EDGVAKLVVGIAAKGNDHIQILSAITNALDDEQAMKTLTSTNDVEKVLALLSK"
BASE COUNT         2570 a         1650 c         1950 g         2530 t
ORIGIN      
        1 aaaatatttt attttgaaaa acggagatcg gtatgattga agcaattaca cacggagcag
       61 aatggtttat cggcctgttc caaaaaggcg gtgaaacctt agtcggtatg ataaacggta
      121 ttttgccact cttaatttca ttattggttg taatgaatgc cctgattaaa tttgtagggc
      181 aagatcggat tgaacgactt gctcaacgta gtgccggaaa ccctatttca cgttatttga
      241 tcttaccggt gatcggcact ttcgttttct gtaacccgat gacattaagc ttagggcgtt
      301 tcttaccgga gcgttacaaa ccaagctact acgccgcagc ctcttatagt tgccactcta
      361 tgaacggctt attccctcac atcaacccgg gtgaattatt cgtttattta ggtattgcca
      421 gcggtttaac caccttaggc ttaccacttg ggcctttagc catcagttat ttattagtcg
      481 gtatgtttac caatttcttc cgtggctgga taacagactt caccaccgca ttctttgaaa
      541 aacgaatggg gatcacctta gatagagaag tacatttatc ccactaacca agtaactgaa
      601 atacagagga atagattatg agcaacaaag cgatttacat tgaaaaaggc aacggtggtt
      661 ggggtggtcc attaacatta cctgttgtgg aaggcaaaaa agtgctttat atgaccggcg
      721 gcactcgccc tgcgattgtt aataaattgg tggaattaac cggctgggaa gcggttgatg
      781 gttttaaaga aggcgaaccg ccggcagaag aaatcgggat tgcgattatt gattgcggtg
      841 gggtattacg ctgtgggctt tacccaaaac gccgtattcc aaccattaac attcactcca
      901 ccggtaaatc cggcccttta gcagaattta ttgtggaaga tatttatgtt tcagcggtga
      961 aagaaaacaa tattcgctta attgatgccg atggaagtgt gcctgtggca acagagcaag
     1021 caaaaccaac ttcatcaacg acagcaacgg caaaagagta tgactccagc aaaaaaatca
     1081 ccgaacaaag tgatggttta ttagcaaaaa tcggtatggg aatgggatcg gtggcggcgt
     1141 tattcttcca agccggacga gacactattg atacggtgct taaaaccatt ttaccgttta
     1201 tggcatttgt ttctgcgtta atcgggatca tcattgcgtc aggtttaggt gatgtgattg
     1261 cccacggctt gacccctctg gcaaacagcc caatcggttt agttacactg gcgttgattt
     1321 gttccttccc tctcctttcc ccattcttag ggccgggagc cgtgattgca caagttatcg
     1381 gcgtgttagt cggtacgcaa atcggtttag gcaatattcc gccacattta gcactgccgg
     1441 cactctttgc tattaatgct caagccgctt gtgattttat tcctgtgggt ctttcaatgg
     1501 cggaagcaaa acaagatacc gtacgggtgg gtgtaccgtc agtattagtc agccgttttt
     1561 taaccggtgc accgaccgtg ctgattgcgt gggcagtttc cgcattcatc taccaataat
     1621 taaccacgat acaagcggtg gaatttgaaa aattttttgc aaaatgcacc gcttgtcaaa
     1681 acgagagaac atttatatga cgattattta tcaaacgacc tttaccaaaa tcgggaattt
     1741 tgctcaggaa tcattaacag acgatatgtt aatcactttt aaacaaggcg caccggcaga
     1801 tttacaagat tactgcttca ttcataatcc atctgaatta agtagccctt tagaagtagg
     1861 cgatatcgct gaatttgatg gcgtagctta tccgattacg gcagttggta gcgttgcatc
     1921 tgaaaactta tccgccctcg gacacattac cttccgtttt gacggggcaa atgatgcgga
     1981 atttccggga agcgttcacg ttatcggaac accgccgcaa ggcttaacag agaactcaac
     2041 cttgattatc aagcgtgatt aatgacggat taaaaataat ttcaaattga aaatttagag
     2101 gaatacatta tgaacaatga aaaaaaagtt gctatcgttg ttggcggcgg tcaaacactt
     2161 ggagcttttt tatctgaagg cttggcagat gccggttatc gtgtggtggt tgctgactta
     2221 aacggcgaaa atgcacaaaa agtggcagcc gacattaatg ccaaacaagg cgaaggaaat
     2281 gcgattgggg tgcaagtcga tgcggctgat gaaccaagcg ttgaagcact agctaaagca
     2341 acagataccg tattcgggcg tgccgatgta ttggtttaca gtgcaggcac agcgaaagca
     2401 tcgcctattt gggatttcaa cttaaacgat tttgatttgt cggtaaaagt gaatttaacc
     2461 ggttatttct taaccgcaaa acacttctcc cgcttaatga ttcgtgacgg tattaaaggg
     2521 cgtatcattc aaattaattc taaatcgggt aaagtaggga gtaagtataa ttccggttat
     2581 agtgcagcga aatttggtgg tgttggttta acacaatcgt tagcattaga cttggcagat
     2641 aaaggcatta ccgtgcattc attaatgtta ggcaatttat tgaaatcgcc gatgttccaa
     2701 tcgttaattc cgcaatatgc ggcaaaactc ggcattccgg aaagcgaagt agagcaagtt
     2761 tatattgata aagtgccttt aaaacgtggc tgtgattatc aagatgtgtt gaatgtctta
     2821 aaattctatg ccggcgaaga ggcagcttat tgcacaggac agtctattaa tattaccggt
     2881 ggtcaggtaa tgttctaagc aaacaagcgg tcaaatttgt cgattttttt acaacaattt
     2941 gcaaaaaatg actaaaataa accgcttgtt aattcgccac accggttaat atgccacagg
     3001 ggaaagcgta ttgttttgaa atcgcaggta cgtttaggct ctgtggcttt ttattaagga
     3061 gcaatgatgg attcaattac acttctgatt atattggcaa tgataatgtg gctgttacaa
     3121 gccgttttgg gttggcttca aattaaagcc tttaaccaag cctttatgga aatcagtaag
     3181 aaaggcaaag taacggttgg gcgtaatagt ggtcgtttta atcctaaatc ggttattgtg
     3241 ttggcgtttg atgatgagaa aaaagtggta gatagccttt gtatgaaagg attttccgtc
     3301 tttgccagac cgcagaaatt agcgtctgtg attggtttgt ctcttgatca aattgtgccg
     3361 gaaaaaatct ttccaagcga taaacgttca caatttgcct taaaagtggc gatatcttcc
     3421 ggatatagtt gctcagatca aaaataatgc gattttttat ttcattttga atattttttt
     3481 agttaaaaat gagataatgg agaataaata tgcagatcaa tagtgtcatc tttgatatgg
     3541 acggggtaat gattgattca gaaccccaat gggcagaggc tcaaattgca gtattagccg
     3601 acttaggtat taatatcacc gagcaagatt gcgaacaact caccagaggt aagcggattg
     3661 atgagttgag cgacacttgg attaaaaaat ttcagttatc tgtttccaac caagcattaa
     3721 gtgaagccat tttggcacac ggttgtgctg cgattgaaca acacggcaaa gcattaacgg
     3781 gtttatatga attattggat ttcttaaaat ctaatcagat caagttagcc gttgcaacct
     3841 catcgccgcc ggtggtgatt aaagcggtgt ttgatcgctt aaaactgtgg gattatttcc
     3901 cgattcgatg cagtgcaatg gacgaagaat atggcaagcc acaccctgcg gtctatttga
     3961 gtgcagtgaa aaaattaggc attgaaaaac aagagtgtat tgttattgag gacagtgtaa
     4021 ccggcttaat tgccgccaaa gccgctaatt taactacatt tttggtgaat accgattatc
     4081 aaaatgaaaa attttcaatt gccgataaaa gaatggcatc gctactagat gtcattgaaa
     4141 aattaaaaca gcattagcac agattagctc gctccaatct ttgctaaatt ggaggaacaa
     4201 tgaaaccttt tgaaagaaga cagcaaattt tagcctattt agctcagcac ggtagaaccg
     4261 aagtggacgt gctggcagac tattttaagc taactggtgc aaccattcgt aaagatttaa
     4321 cttcacttga agaagataag aaagtcttgc gtacttatgg cagtgtagtc ttaattgatg
     4381 aagaaattct tgaacgggaa gagaaaatca ataaagagga caaagacgat aaaccgcttg
     4441 cacagaaaac cggcattaat cttcaacaaa agcaagctat cgggcaaaaa gctgcagagt
     4501 taattaatga gggaaactct attatttttg atgcaggcag cacagtgtta caaatgattc
     4561 cccatttaaa agcatttgat aacctctctt taatgacaaa tagtttaact attttcaatg
     4621 agattttgaa actcaataaa tcctataact tactgatttc cggtggtaca tttcgtgaga
     4681 aatcagcttc atttcacggt tattttgctg aatccgcttt tattggttca aattttgaca
     4741 tactctttat tggcacagac ggactagatt tagacgttgg attaacgact ttcaatgaag
     4801 tatataaagt cagttcgcta atgtgtgatg cggcaaataa aatcgtctta cttgccgact
     4861 ccagtaagtt tggacgtaaa agtccgaata ttgtgtgtgg gttagaaaaa attcatacta
     4921 tcattaccga tgaaggatta tctcaagaaa tgagagaaca gattaaagcg aaagggattg
     4981 agctgttagt tgtataaggg cgtaaaaatg aatcatttag acagtgcaaa agaaacgtta
     5041 aacctctatg ctcagcagat tatcacgcta aaccatcgac tttctgaaga atttaatgag
     5101 gtggtaaata tgatactcgc ttgtcaaggg cgagtggttg ttggcggtat tggaaaatcg
     5161 gggctggttg gcaaaaaaat ggtggcaact tttgcctcca ccggtacacc gagctttttc
     5221 ttgcacccga cagaggcttt tcacggcgat ttgggaatgc tgaaaccgat tgatatggta
     5281 attctgattt ctaacagtgg tgaaactgat gatgtgaata aactgattcc aagcctgaaa
     5341 aatttcggca ataaaattat tgcgatgaca ggtaatcctt actccacact tgggcgaaat
     5401 gccgatgtga ttttaaatat tggtgtagag cgtgaagcct gcctgaataa tctagctcca
     5461 accagctcta ctttggtaac gatggcttta ggcgatgcgt tagccattgc gctaatgaaa
     5521 gcccgtgatt tccgccctga agattttgcc cgctaccacc cgggtggcag tcttggtcgt
     5581 aaattattga acagagtgcg agatgtgatg gtgcgtaaag tgccaaccgc cagcttagac
     5641 accaccttta ccgaatgttt atctgtgatg aacgaagggc gtatgggggt ggcggtaatt
     5701 atgcaagacg ataaattgga aggcattatt accgatggtg atatccgccg cacattagcg
     5761 aaattcgggg cggagagcct aaataaaacc gctgacgaaa ttatgacccg taatccaaaa
     5821 accattaacg ataccgaatt tttagccaaa gccgaagatt taatgaaaga gctgaaaatt
     5881 cactcattaa ttgcagtgaa tgatgagggt aaagtaacgg gattgatgga gttctctagt
     5941 taatttatag atgtaacgat ggcgcaagcg tttacgcttg tgccgttcac tgacatatat
     6001 caatatctaa taaaacaggt aagttgttag cataatttct tgcactgcta tatttccatt
     6061 caaatgcctc gcatacaaaa ccgcttacga tagggttgtt gtgaatatgg tttagctttt
     6121 catcaaaaaa tttctgagaa tagattgtta tcggttggtt atgatgttgc caaagttggt
     6181 attgcttgac attatttcgt tgtttagctg cccgagtaaa catccataat aaccattctt
     6241 ttctactttc ttggggattt gttttaattt gctgaatcaa catttagatg taaatgtttt
     6301 aaaatctctt attaaatctg acgggttttg gtcttttgct tgaaataata aatgaatatg
     6361 gcttggcata atgcagtagc catataaaat catccctttt tgttttcgac aataattcaa
     6421 tgattggata atggtttgaa aataatcttc tcgaataaat agatcaatcc aataaactgt
     6481 tgcaaaacta acaaaatagg ctccgctctt ctcataaaac ttatattttc tactcattat
     6541 gaacctctaa agtgcagttt atgctgatag taatataggt gaacagttag ggttagcaat
     6601 tcattttatt ttttcgatgt agatcgcaaa aatagaatta tgcttggcac aagcgaggac
     6661 gcttacgcca tctttactcc aaaaatgtga gatagatcac attttcaaat ctgcccaaaa
     6721 aaatttaatg tgatctactt cacatttctg agagaaacat ttttgtaact tttgaaagtg
     6781 gtgctaattt aagcacgatt ttttcaaccc taattttctc tccttttttg aggtttctat
     6841 gtctaattta aaagtgaaag tccaaggttt tgggcgtttc ttatcgaata tggtgatgcc
     6901 gaatatcggt gcctttattg cgtggggctt tatcaccgcc ctgtttattc caaccggttg
     6961 gttgccgaac gagacttttg ctaaactggt cggtccgatg attacttact tgctgccact
     7021 gttaatcggt tacagcggtg gtaaattagt cggtggtgag cgtggtgcgg ttgtaggtgc
     7081 gattactaca atgggtatta tcgtgggttc tgaaatcccg atgttcttag gtgcgatgat
     7141 tgtaggtcca ttaggcggct gggcgatcaa aaccttcgat aaagcgattg acggcaaagt
     7201 aaaaagcggt tttgagatgt tggtaaacaa cttctcggtc gggatcatcg gtatgatttt
     7261 ggcaatgctt tcatttgctt tcattggagg tatcgtcacc caaatctctg cggcattagg
     7321 tgcaggtgtg ggggcgttgg ttgaagctgg tcttctgcca gtaacctcta tcatcgttga
     7381 accggcgaaa atcctattct taaacaatgc gatcaatcac ggtgtgttca cgccattggg
     7441 tacagcacaa gcacaagaag tgggcaaatc tattctgttc ttgattgaag cgaacccggg
     7501 tccgggcttt ggtatcttgc ttgcctatat gttctttggt cgtggcacag caaaacaaac
     7561 cgctggtagt gcgtcaatca ttcacttctt aggcggtatt cacgaaattt atttcccgta
     7621 tgtgttaatg agcccacgct taattcttgc agtgattgca ggcggtgcaa ccggcgtttt
     7681 aaccaactca ttattaggtg gcggtttaat cgcaccggct tccccgggtt ctatttttgc
     7741 gattttagca atgaccgcac cgggtgctca ctttgcagtt gttgcctccg tgattctttc
     7801 ttgtgccgtg acttttgcga ttgcgtcaat cttactcaaa acccaaaaag gtgaagcctc
     7861 attggaagat gcacaagcac aaactcaagc gatgaaagca gaaagcaaag gcgtagtaaa
     7921 tggtaacatc aacaaaatct atgtagcgtg tgatgccggt atgggttcaa gtgcgatggg
     7981 tgcgagtatg ttacgcaaaa aagtgcaagc ggcaggttta aacattgaag tacaaaactt
     8041 agcgatcaac gacttaccgc aagacgcaca actggtgatt acccacaaag acttaaccgc
     8101 tcgtgcaaca caacgtgtgg caacggctca acacttatcg ctcactaact tcttagacag
     8161 tgcgttctat gacagcttag tggcaaaact ggctaaccac cagccacaac aagcggtgca
     8221 aaatacacaa aattttgcaa acccggtgga agaacctcaa gccgaagggt taaaattgca
     8281 agcagcccaa gtctttttag gcttacaagc taacagtaaa gaagaagcga tccgctttgc
     8341 cggtgagcag ttagttaaag cgggctttgt tcaaccaagc tatgtagatg caatgttcgc
     8401 ccgtgagcaa atggtttcaa cttacttagg cgaaggctta gctgtgccac acggtacatc
     8461 tgatgccaaa gacagcgtac tgaaaaccgg tatcgtggtc tgccaatacc cgaacggcgt
     8521 tcgcttcacc gatgaagaag acggcgtggc gaaactggtg gtcggtattg cagcaaaagg
     8581 caacgaccat atccaaattt taagtgcaat taccaacgca ctggacgatg aacaagcaat
     8641 gaaaacactg acctcaacaa atgacgtgga aaaagtgtta gcattattat ctaaataatc
//
LOCUS       DANLRK010000108         8648 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.108, whole genome shotgun sequence.
ACCESSION   DANLRK010000108 DANLRK010000000
VERSION     DANLRK010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8648
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.108"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            60..329
                     /locus_tag="PXA33_001790"
     CDS             60..329
                     /locus_tag="PXA33_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005540356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL5121100.1"
                     /translation="MKIMAVCGSGLGSSFMMEMNIKKVLAKLGVEAEVSHTDLSSVVA
                     DDADLFVMAKDIAASSNVPKEKLIVVKNIVGLAEFEEKLTAYFNQ"
     gene            342..1692
                     /locus_tag="PXA33_001791"
                     /pseudo
     CDS             342..1692
                     /locus_tag="PXA33_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013745980.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
     gene            complement(1787..2935)
                     /gene="dprA"
                     /locus_tag="PXA33_001792"
     CDS             complement(1787..2935)
                     /gene="dprA"
                     /locus_tag="PXA33_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HDL5121101.1"
                     /translation="MAYHYDLLRLLQVPQMGAQRIGRLLAELNFAEFCHYDKLQLKQI
                     GWNEKQIQRWFNPDQKWIDQAMTWSELPNQQIVTLFDENYPFLLKQISTAPPLIYVKG
                     NVESLSLPQIAIVGSRDYSHYGEYWAGQFAAELARHNIIITSGLAIGIDGFAHQKIVM
                     EQGITIAVLGSGLNQVYPARHKKLAEQIVDLGGALVSEFSPNQPPVAENFPRRNRIIS
                     GLSLGTLVIEATLNSGSLITARYALEQGREVFALPNAVQNPYAQGCHKLIKEGALLVE
                     GVEDILEVVASQYQPQQIPLFDESKPTVSTSLQAVKNSEKFAKNLPSLTACQQQIYQH
                     ISLEPMAIDDLAKVTELTIEILLVELLGLELAGVIKQVSGGYVMDNGY"
     gene            3024..3536
                     /gene="def"
                     /locus_tag="PXA33_001793"
     CDS             3024..3536
                     /gene="def"
                     /locus_tag="PXA33_001793"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005621146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDL5121102.1"
                     /translation="MAILDVVLYPDEKLATVCEPVEQVDEALNRFIDDMFETMYEHEG
                     IGLAAPQVGVLKRVITIDIEGDKTNQVVLINPEILESSGETGIEEGCLSIPGHRALVP
                     RKEKVKVKALNRQGEEIIIDADDLFAICIQHEIDHLNGVLFVDHISNLKRQRIKEKMQ
                     KLKKQIARAK"
     gene            3630..4556
                     /gene="rfaD"
                     /locus_tag="PXA33_001794"
     CDS             3630..4556
                     /gene="rfaD"
                     /locus_tag="PXA33_001794"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HDL5121103.1"
                     /translation="MIIVTGGAGMIGSNIVKALNEIGRKDILVVDNLKNGEKFINLVD
                     LDIADYCDKEDFIASIIAGDDFGEIDAVFHQGACSATTEWDGKYLMQNNYEYSKELLH
                     FCLDRQIPFFYASSAATYGGRSDNFIEKREFEGPLNAYGYSKFLFDEYVRRILPEAES
                     PVCGFKYFNVYGPREQHKGSMASVAFHLNNQMLKGENPKLFAGSEQFLRDFVYVEDVA
                     KVNLWAWQNSISGIFNLGTGKAESFQEVANAVIKYHGKGEIETIPFPDHLKSRYQTFT
                     QADLTNLRAAGYTGTFKTVAEGTAEYMAWLNR"
     gene            complement(4624..5154)
                     /gene="fabA"
                     /locus_tag="PXA33_001795"
     CDS             complement(4624..5154)
                     /gene="fabA"
                     /locus_tag="PXA33_001795"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017807287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HDL5121104.1"
                     /translation="MNSCTPIIKPSYTYEELLLSGKGELFGEKGPQLPAPPMLMMDRI
                     IEMNQEKGNFEKGYIEAEFDIKPDLWFFNCHFINDPVMPGCLGLDAMWQLVGFYLGWI
                     GGEGKGRALGVGEVKFTGQILPTAKKVTYRIHMKRVVNRKLVMGVADGEVEVDGRVIY
                     TATDLKVGLFQDTTAF"
     gene            complement(5267..6904)
                     /locus_tag="PXA33_001796"
     CDS             complement(5267..6904)
                     /locus_tag="PXA33_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL5121105.1"
                     /translation="MTLSLLSSQKLVMQNDFSRLDEKLNFLDFQPQAKQALNQFLQSQ
                     FGALLVLKSELFPEFINEITEYLKKYSVDLPIISKMDFNKNSLFGYSVFLERELKTEY
                     VKGAIHEADKGILIVNIRSLLLDITQWDKLKQALLFGEYEPNSVNHSPYLSAKEKSTF
                     KLILVGEREDISTLSAYDDGLYQFGQYTEIKSYLSLSDNVEQWGGYVQNYAENLINKR
                     LTSTALNQFLQAYIRESESRELVSISPTLLRKHLLGIQNFYPNVTEIDEVKGYFDYLE
                     QQSSILNQYTVGDILTNQLYIETEGEEIGQINGLSVIEFEGIPHSFGEPLRMSCNVQY
                     GDGEITDIERKVELGGNIHSKGIIIAQSCLANLLELPTQLPFSASIAFEQSYGEIDGD
                     SSSLAIFNVLVSALAKVGLPQSIAVTGAIDQFGNVLSVGGVNQKIEGFFNICHARQLT
                     GKQGVIIPAACVSHLSLSSEVVEAVEQGLFKIWTVENVFDAIAILFERDFYDDEKAPN
                     SAQTALFTLIHQNLDERESEETSGSFLSKICKKIKLD"
     gene            6997..7443
                     /gene="matP"
                     /locus_tag="PXA33_001797"
     CDS             6997..7443
                     /gene="matP"
                     /locus_tag="PXA33_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010944225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HDL5121106.1"
                     /translation="MRYQKLITQETTWKWQYLLKKHREGENITKYEEQSLIDLKVQFL
                     STLQNSPDEVEKWIKSEMTPEQRKKMRQSVRAKRKRFFNAEKQTTKKKSIDLEYSSWL
                     RLSKYAKSQKLTLSEAIVKLTDELENKQLYLEQVAKMKSSLKDLLK"
     gene            complement(7509..8645)
                     /gene="ompA"
                     /locus_tag="PXA33_001798"
     CDS             complement(7509..8645)
                     /gene="ompA"
                     /locus_tag="PXA33_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_119863613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HDL5121107.1"
                     /translation="MKKTLVALAVLSAAAVAQAAPQANTFYAGAKAGWASFHDGLTQF
                     DNTYDYGSGINLGINLGINRNSVTYGVFGGYQILNQNNFGLATELGYDYFGRVRGNTT
                     FSGDDKEKTAAKHSAHGAHLSLKPSYEVVPNLDVYGKVGVALVRNDYYVQQHVDKDSR
                     IKVHNLKPSLLLGAGLEYAITPELAARVEYQYLNRVGNLDKAVRTTVGYLIPRGTNFQ
                     YSPDIHSVSAGLSYRFGQGAAPVEAPEVVTKNFAFSSDVLFDFGKSSLKPAAATSLDA
                     AHAEISNLGLANLAIQVNGYTDRIGKEASNLKLSQRRAETVANYIVSKGTNPANVTAV
                     GYGEANPVTGHTCDAVKGRKALIACLAPDRRVEIQVQGSKEVTM"
BASE COUNT         2602 a         1788 c         1650 g         2608 t
ORIGIN      
        1 agtatttcta tagttcaaaa ttcagacaac tattcattca cctttaatgg agatttacta
       61 tgaaaattat ggcagtatgt ggttccggct taggtagcag ctttatgatg gaaatgaaca
      121 ttaaaaaagt attggcaaaa ctcggtgtcg aagcggaagt gagtcatacc gacctttcct
      181 ctgttgttgc tgatgatgct gatttatttg tgatggcaaa agatattgcc gcaagcagca
      241 atgtgccgaa agaaaaactc atcgtggtaa aaaacattgt tggattagca gaattcgaag
      301 aaaaattgac cgcttacttt aaccaataac ggagatgtta tatggaatcg ctactctttt
      361 ttatattaga tattctcaaa gtgccttccg ttctagtggg tttaattgct ctggtcggtt
      421 tagttgcaca gaaaaaatcg gtatccgata ttgttaaagg aacaatcaaa actattttag
      481 gcttcttagt gttaagcggt ggtgcaacag tacttttaag ctccctgact cctttaggcg
      541 gtatgtttga acacgccttc aacgtgcaag gcattatccc taataacgaa gcgattgtgt
      601 ctatggcaat cgaaaaatat ggtacggcga ctgcgttaat tatggcattc ggtatggtag
      661 ccaatatttt aattgcacgt tttacccgcc taaaattcat tttcttaacc ggacaccata
      721 ccttctatat ggcgtgtatg atcggcgtaa tcctaaccgt tgccggcttt gaaggtattc
      781 agctcgtctt tgtcggggca ttaaccttag ggttaattat gtttcccgac tatcgctcac
      841 ttctatatga aaaaagtgac cgctagcaat gatgttgcct taggtcattt cggcacattg
      901 ggctacgtgc ttgctggttc aatcgggcaa ctggtaggaa aaggctctaa atctaccgaa
      961 gaaatggatt tacctaaaaa cctcggcttc ttacgtgata gctccatttc tatttcactc
     1021 acgatgatgg cgatttacta tattctcgcc cttgcttcgg gcagtgaata cgtttctaca
     1081 ttaagcggcg gacaacatta tttagtgtat gccaccattc aagccatcac ttttgcagcc
     1141 ggtgtttatg tcattttgca aggggttcgt ttaattttag ccgagatcgt cccggcattt
     1201 accggcttct ccgaaaaatt agtacctgat gccaaaccgg ctctcgactg cccgattgtt
     1261 ttcccttacg cccccaatgc ggtattagtt ggatttcttg ccagtttctt aggtgggatt
     1321 ttaagcttag ccattctagg gcaacttaat tgggtgttaa ttttaccggg cgtagtgcca
     1381 cacttcttct gtggggcaac tgccggcgta tttgggaacg ccaccggcgg gcgtcgtggt
     1441 gcaattatcg gtgcttttat tcacggcata ttaattacct tcttgcctgt attcttatta
     1501 cctgtactgg gttcattagg ctttgctaac accactttct ctgactcaga cttcggcggt
     1561 attgggattg tgttaggcta tatggctcaa tacttcgaca agaatatcat tacgctcatt
     1621 attgtcggct tattcgctct gttggtagcc tataattatt tggcgaaaaa accggcaaaa
     1681 ctgaatacct aattaaaatc aggtgttatt tgctgcctgc gaggaatctt ccctgccatt
     1741 tcttgatggt agggaatttt gtttagttaa acgacaacgc tcttgttcaa tacccattat
     1801 ccatcacata ccccccactc acttgtttaa tcacaccggc taattccaga cccagtaatt
     1861 caaccagcaa tatttcaatc gtcaactccg taactttggc taaatcatca attgccatcg
     1921 gctctaagct gatatgctga taaatttgct gctggcaagc tgtcagactt ggcagatttt
     1981 ttgcgaattt ttcgctgttt ttgaccgctt gtaaagatgt gcttactgtt ggcttagact
     2041 catcaaacaa agggatttgc tgaggttgat attgcgaggc gaccacctct aaaatatctt
     2101 ccacaccttc cacgagcaat gccccttctt taattagctt gtggcaacct tgtgcgtaag
     2161 ggttttgtac cgcattcggt aacgcaaaca cttccctacc ttgttcaagg gcataacgag
     2221 cggtaatcag tgaaccacta ttaagcgttg cttcaataac taaagtgccg agcgataagc
     2281 cactaataat tcgattacga cgaggaaaat tttctgccac cggcggctga ttaggcgaga
     2341 actccgacac caatgcacca cctaaatcaa caatttgttc ggctaatttt ttatggcgag
     2401 caggatagac ttgatttaat ccactcccta acacggcaat cgttatgcct tgttccataa
     2461 cgattttttg atgggcaaaa ccgtcaatac caatggctaa accgctggta atgataatat
     2521 tatggcgagc taattcggca gcaaattgcc cagcccaata ttcaccataa tgagaatagt
     2581 ctcggctgcc aacaatcgca atttgtggca gagaaaggct ttccacattt ccctttacat
     2641 aaattaacgg tggcgcagtc gaaatttgtt ttaagagaaa cggataattt tcatcaaaga
     2701 gtgtcacaat ttgctgattt ggcaattctg accaagtcat cgcctgatca atccactttt
     2761 gatcaggatt aaaccaacgt tgaatttgct tttcattcca acctatttgt ttgagttgaa
     2821 gtttgtcata gtggcaaaat tcagcaaaat tgagttccgc caataatcgc cctatacgtt
     2881 gagcccccat ttgcggcact tgtaataatc gtaataaatc gtagtgatat gccatatttt
     2941 catcctcaaa atttccctaa taataagaaa aggtttaaaa tagccaaacg aattttttaa
     3001 acttttcgag agagatcaca aaaatggcaa tattagatgt tgtgctttac cccgatgaga
     3061 aactggcaac cgtttgtgaa ccggtggaac aggtggacga agcactgaat cgttttatcg
     3121 atgatatgtt tgagacaatg tacgaacacg aaggtattgg cttagctgca ccgcaagtgg
     3181 gcgtacttaa gcgtgttatt actattgata ttgagggcga caaaaccaat caagtggtgt
     3241 taattaaccc tgaaatttta gaaagcagcg gtgaaaccgg cattgaagag ggctgtctct
     3301 ccattcccgg gcatcgtgcc ttagtgccac gcaaagagaa agtgaaagtc aaagccctta
     3361 accgccaagg cgaagaaatt atcatcgatg ccgatgattt atttgcgatt tgtattcaac
     3421 acgaaatcga ccatttaaac ggcgtgcttt ttgttgacca catatccaat ttaaaacgcc
     3481 aacgcatcaa agaaaaaatg caaaaattga aaaagcaaat tgcacgagcg aaataaccag
     3541 gcacaagcgg ttaaaatggc ttaaaaattt gcaaattaag tgtgatgaca cgataataac
     3601 cacagttcaa ctttagaaag gataaaaata tgattatcgt caccggcggc gcaggtatga
     3661 ttggcagtaa cattgttaaa gccttaaatg aaatcggtcg caaagatatt ttagtagtag
     3721 ataacctcaa aaacggggaa aaatttatca acttagtgga cttagatatt gcggattatt
     3781 gcgataaaga ggatttcatc gcctctatta tcgccggcga tgattttggt gagattgatg
     3841 cggttttcca tcaaggggct tgctcggcaa ccaccgaatg ggacggcaaa tacttaatgc
     3901 aaaataacta cgaatattca aaagagttat tgcatttttg cttagatcgc caaattcctt
     3961 tcttctatgc ctcaagtgcg gcaacctatg gcggacgttc ggataatttt atcgagaaac
     4021 gggagtttga aggtccatta aatgcttatg gctactccaa attcctgttt gatgaatatg
     4081 tccgccgtat tttgcctgag gcagaatcac cggtctgtgg tttcaaatat ttcaacgtat
     4141 acgggcctcg tgagcaacac aaaggttcaa tggcaagcgt ggcattccac ttaaacaacc
     4201 agatgttaaa aggggaaaat ccaaaactat ttgccggctc agagcagttc ttgcgtgatt
     4261 ttgtttatgt ggaagatgtg gcaaaagtga atctttgggc gtggcaaaac agcatttctg
     4321 gcattttcaa cctcggcacc ggaaaagcgg aatctttcca agaagttgcg aatgcggtta
     4381 tcaaatacca cggcaaaggc gaaattgaaa ccatcccatt cccggatcat ttaaaatccc
     4441 gctaccaaac gttcactcaa gcggacttaa ccaacctaag agccgctggc tacacaggca
     4501 catttaaaac cgttgctgaa ggaacagcgg aatatatggc gtggttgaat cgctaatcaa
     4561 acgttgttaa aaaagcagaa agagctacga aatgtagctc tttttgttat aaggttataa
     4621 ggattagaat gctgtcgtat cttggaataa gcctactttt aaatcagtag cagtatagat
     4681 cactcgcccg tccacttcga cttcaccatc agcaaccccc atcactaact tacgattgac
     4741 cacacgtttc atatggatac ggtaagtaac ttttttcgct gtcggtaaaa tttgtccggt
     4801 gaattttact tcacctacgc ccaatgcacg ccctttgcct tcgccaccaa tccagccgag
     4861 ataaaaacca accagttgcc acatcgcatc taagcctaag caacccggca ttacaggatc
     4921 gttaataaaa tggcagttaa aaaaccataa atccggttta atatcaaact cagcttcaat
     4981 ataccctttt tcaaaattgc ctttttcttg gttcatttca atgatgcgat ccatcattaa
     5041 catcggtgga gccggcaatt gaggcccttt ttcaccgaat aattctcctt ttcctgatag
     5101 caaaagctct tcgtaagtat agcttggttt gataattggt gtacaactgt tcattatttt
     5161 taaaatcctg taaagtgaaa aaagcctgtt atatcaataa ctgataaatc aggctgatag
     5221 taacagactt ttaattcagt gaacagttgt aagctgactt gtcggatcag tctagcttta
     5281 tctttttgca aattttgctt aaaaatgaac cgcttgtttc ttcactttcc cgctcatcta
     5341 gattctggtg aatcaaggta aataatgctg tttgggctga gttaggggct ttctcatcat
     5401 cataaaaatc ccgctcaaac agaatcgcta tggcatcaaa cacattttcc accgtccaaa
     5461 ttttaaatag accttgctcc actgcctcaa caacttctga acttaaactc aaatggctaa
     5521 cacaggccgc cggaataatc acaccttgtt tccccgttaa ttggcgagcg tgacaaatat
     5581 taaagaagcc ttcaattttc tgatttaccc cgcccacgct tagcacattc ccaaattgat
     5641 cgatagcacc tgtcaccgca atagattgtg gcaagccaac tttagctaag gcactcacta
     5701 gtacattgaa aatcgcaaga gaagaactat ccccatcaat ttcaccatag gattgttcaa
     5761 aagcaatcga agctgaaaac ggtaactggg ttggtagttc taacagattc gctaaacaag
     5821 attgagcgat aataatacct ttggagtgga tatttccccc tagctccact ttacgctcaa
     5881 tatctgtaat ttcgccatca ccatattgta cattacaact catgcgtaag ggctcgccaa
     5941 aactatgcgg aatgccttca aactcaatga ccgataagcc attgatctgc ccaatttcct
     6001 ctccttcggt ttcaatataa agttgattag tcagaatatc acccaccgta tattgattta
     6061 atatcgatga ttgctgttct aaataatcaa aataaccttt tacctcgtca atttcggtca
     6121 catttgggta aaaattttga attcctaata aatgttttct caataatgta ggggaaatag
     6181 acaccaactc tcggctttca ctttcacgta tataggcctg taaaaattga ttaagcgctg
     6241 tggaagttaa tcgcttattg atgagattct cagcataatt ctgaacataa cctccccact
     6301 gctccacatt atccgataat gataagtagg attttatttc ggtatattgc ccaaattggt
     6361 acaacccatc atcataagca gataaagtag aaatatcttc tctttcccct actaaaatca
     6421 atttaaatgt tgatttctct ttagccgata aatagggtga gtgattaaca gaatttggct
     6481 catattcacc aaataataat gcctgtttta gcttatccca ttgggtaata tccaataaga
     6541 gcgaacggat attaacaatt aaaattccct tatctgcctc gtgaattgca cctttaacat
     6601 attctgtttt cagctctctt tccaaaaaca cagaataacc aaatagacta tttttattga
     6661 aatccatttt gctaataatc ggcagatcta cactatattt tttcagatat tccgtgattt
     6721 cattgataaa ttccggaaaa agctcagatt ttaagactaa taaagcacca aactgagatt
     6781 gtaaaaattg atttaacgct tgttttgctt gaggttgaaa atctaaaaaa ttcaatttct
     6841 catctaaacg agagaaatca ttttgcatta ctaatttttg gctactaagt aaagaaaggg
     6901 tcacaaatct gcctcattat tgaaaatttg gtttattata caaaattccg ctatacttgc
     6961 taaatatagc ttactttttc ctatcatatt cagattatgc gatatcagaa actcatcacc
     7021 caagaaacaa cttggaaatg gcaatatctc ctaaaaaaac atcgtgaagg agaaaatatt
     7081 actaagtacg aagaacaaag cttgatcgat ttaaaagttc agttcctctc tactttgcaa
     7141 aactctccgg acgaagtgga aaaatggatt aagtcggaaa tgacgcctga gcaacgtaag
     7201 aaaatgcgtc aatcagtacg cgccaaacgc aaacgcttct ttaatgctga aaagcaaaca
     7261 accaagaaaa aatccattga tttagagtat tcgagctggt taaggctctc taaatatgcc
     7321 aaaagccaaa aactaacgct ttctgaagct attgtcaaat taaccgatga actcgaaaat
     7381 aaacaacttt acttagagca agttgctaaa atgaaatcaa gtttaaaaga tttactgaaa
     7441 taaaaaaaga aaaaagccca ctaacaagcg ggcttttttt gattcaattt ttcaactgaa
     7501 taactatatt acatagttac ttcttttgaa ccttgaactt ggatttcaac acgacgatct
     7561 ggtgctaagc aagcgattaa tgctttacga cctttaactg catcacatgt gtgaccagtt
     7621 actgggtttg cttcaccgta acctacagct gttacattag ctgggttagt acctttagaa
     7681 acgatgtaat tagctactgt ttcagcacga cgttgtgaaa gttttaagtt agatgcttct
     7741 ttaccgatgc ggtctgtgta accattaact tggatagcta ggtttgctaa gcctaagttg
     7801 ctgatttcag cgtgtgctgc atctaaagaa gttgctgctg ctggttttaa gcttgattta
     7861 ccgaaatcaa ataaaacatc agagctgaat gcgaagtttt tagttacaac ttctggtgct
     7921 tcaactggtg ctgcaccttg accgaaacgg tatgataaac ctgcagatac tgagtggata
     7981 tctgggctgt attggaaatt tgtacctctt ggtattagat aaccaactgt tgtacgaact
     8041 gccttatcta agttaccaac acgatttaag tattgatatt caacacgagc agctaattca
     8101 ggagtaattg catactcaag accagcacct aataatagag atggttttaa gttgtgaact
     8161 ttaatgcgtg aatctttatc tacatgttgt tgtacgtagt aatcgttgcg aactaatgca
     8221 acacctactt taccgtaaac atctaagtta ggaacaactt cgtagcttgg ttttaagctt
     8281 aagtgcgcac cgtgagctga atgtttagcc gcagtttttt ctttatcatc accagaaaat
     8341 gtagtgttac cacgaacacg accaaagtag tcataaccta attctgtcgc taaaccaaag
     8401 ttgttttggt ttaagatttg gtaaccaccg aatacaccgt aagttacaga gttacggtta
     8461 ataccgaggt taataccgag gttaataccg gatccataat catatgtgtt gtcaaattga
     8521 gttaaaccgt cgtggaatga tgcccaacca gctttagcac ctgcgtagaa agtgttagct
     8581 tgtggagctg cttgagctac tgcagctgct gataatactg ctaatgcaac taatgttttt
     8641 ttcatttt
//
LOCUS       DANLRK010000109         8510 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.109, whole genome shotgun sequence.
ACCESSION   DANLRK010000109 DANLRK010000000
VERSION     DANLRK010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8510
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.109"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(<1..627)
                     /locus_tag="PXA33_001799"
     CDS             complement(<1..627)
                     /locus_tag="PXA33_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121108.1"
                     /translation="MRIWQKLASVIVVTLGLISNASAENFKLKYSSNYLMPAYINFNN
                     GGGQYNIQAKINVPLYNIVFSAKGTEKNEQFHMLSYRDTRNGKTYAMAEIDSKTVKYG
                     KVKEPLKEEALTMPTYDLFTTAFQLSYYDKLPSSFQTTNGKKLYPTPNVQLRKAQKEV
                     KVGGKTYQEITYKFRTSDKKDITVKKFEGEKFPRYIAYSRDGDNYELTF"
     gene            complement(628..1611)
                     /gene="cysB"
                     /locus_tag="PXA33_001800"
     CDS             complement(628..1611)
                     /gene="cysB"
                     /locus_tag="PXA33_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HDL5121109.1"
                     /translation="MKLQQLRYIVEIVNQNLNITEAAEVLYTSQPGISKQVRLLESEL
                     GINIFERNGKHVKGLTPAGEKIVAIARELLVKAQSIKSVAEEQTRPNKGILRIATTNT
                     QARYMLPSVIEKFKARYPEVSLHLHQGSPNQIYDALVAGEVDLAITTEAQYLFDEAVL
                     LPCYWWNRSVIVKPEHPLAAFVNQSKNLTVEQLGRYDLITYTFGFTGQSDLDHAFNKE
                     GILPNIVFTATDADVIKTYVRLGLGVGIMASMAHTETDKDLVAINASHLFQPSMTQIA
                     FKRGTFLRNYMYDFINYFSPHLTRVQVEKAEQLRDNNAISRLFESISLEEK"
     gene            complement(1608..2291)
                     /gene="yjjG"
                     /locus_tag="PXA33_001801"
     CDS             complement(1608..2291)
                     /gene="yjjG"
                     /locus_tag="PXA33_001801"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HDL5121110.1"
                     /translation="MKYQWVLFDADETLFSFNSYLGLKAMLARYQIDFSEQDYQDFQA
                     VNKPLWVAYQNNEITAQDIQTRRFVKLSAQTGVDPLQLNQELMAEMAFVSQPLEGVMD
                     MLNALYGKVKMGIITNGFTDLQQKRLDNTQTSHFFDIVVISEQIGAAKPDRQVFDYAF
                     ALMDEFDRTKVLMVGDTLASDILGGNNAGIDTCWLNLLSKENDTDIKPTYEVHTMQQI
                     IAIVEGKSL"
     gene            complement(2367..3260)
                     /locus_tag="PXA33_001802"
     CDS             complement(2367..3260)
                     /locus_tag="PXA33_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GcvA"
                     /protein_id="HDL5121111.1"
                     /translation="MNKKLPPLNALKAFESAARHLNFTKAAEDLFVTQAAVSHQIKLL
                     EDFLGASLFHRRNRLLELTELGAQYFYEVQPLLEQIALATDKIRQQSSRQILTISVPQ
                     TFGMHWLVPRLADFHEKYPEVEVRIKGVDQDEGLLGKETDVAIYYGKGDWQGLESIRL
                     TESRIVILASPEYLSQNTIHLPADLAGKHLLHSSTHNKWKRMVEHLHIEDIVDAETGS
                     IFSHTFMVLQAAMHQQGIAIANQLLAQHEIEKGTLIEPFPTTLFDEKSFYVVYPSQSA
                     QVQKVQFFVEWIQQKMKECCA"
     gene            3706..4743
                     /gene="rsgA"
                     /locus_tag="PXA33_001803"
     CDS             3706..4743
                     /gene="rsgA"
                     /locus_tag="PXA33_001803"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HDL5121112.1"
                     /translation="MSKPKLTHNQQRRIQSNHHKRINKKEIEWQDEMLGEMQQGIVVT
                     RHVKHADVETAEGQIFRCNIRRTLKNVVVGDQVSWRKGSEQLQGISGVIEAVFPRKNE
                     LTRPDYYDGIKVMASNIDQIIIVSAVLPTLSLNIIDRYLVICETANIPALIVLNKIDL
                     LNESERQAVKEQLKIYEEIGYETLCLSADTGENMQALHQYLAKGTSIFVGQSGVGKSS
                     LINQLLPEVNALTGSVSDNSGLGQHTTTASRLYHLPQGGQLIDSPGIREFGLWHLEPE
                     QITLGYREFQSVLGTCKFRDCKHKTDPGCALRQEVENGEISAIRFENYHRLIESLSET
                     KSQRHFRKEEV"
     gene            4740..5339
                     /gene="rnhB"
                     /locus_tag="PXA33_001804"
     CDS             4740..5339
                     /gene="rnhB"
                     /locus_tag="PXA33_001804"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HDL5121113.1"
                     /translation="MMNEFIYPNARLIAGVDEVGRGPLVGAVVTAAVILDPNNPIQGL
                     TDSKKLSEKKRQALAEEIKAKALSWSLGRAEPTEIDELNILNATMLAMQRAVAGLHIQ
                     PDFVLIDGNRPPKLPMPAQAVVKGDSLVAEISAASIIAKVARDQEMDELDQQFPQYGF
                     AQHKGYPTKLHFEKLAEFGATPYHRKSFAPVRKVLGLID"
     gene            5352..5711
                     /locus_tag="PXA33_001805"
     CDS             5352..5711
                     /locus_tag="PXA33_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HDL5121114.1"
                     /translation="MTTEYQKTIFTKIINKEIPANIVYQDELVTAFRDIAPQAPTHIL
                     IIPNKFIPTVNHVSNEDELALGRLFTVAAKLAQEEGIAEEGYRLVMNCNKNGGQEVFH
                     IHMHLLGGQKLGPLVAK"
     gene            5714..6085
                     /locus_tag="PXA33_001806"
     CDS             5714..6085
                     /locus_tag="PXA33_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HDL5121115.1"
                     /translation="MRISYFAFAIFSIFLTACGSKPVSYLPTGSEPIVNIESDIAEKI
                     ELETNSKQFSTTNLTEFPLNLTYKLFWYNKDGVTQSHNGISDSIGWQHLWLQPKQKQT
                     IALIKPTEESHNYRLYLRGVK"
     gene            complement(6120..6749)
                     /locus_tag="PXA33_001807"
     CDS             complement(6120..6749)
                     /locus_tag="PXA33_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL5121116.1"
                     /translation="MFNLQIIPVSPFQQNCTVIWDENKNAAIIDGGDNAEGIIQFVES
                     QQLKVQKLLITHGHLDHIMAVEKLREHFQVEVFGSHLADKPLFERLPEMCQQFGLPPV
                     NGFLPDHWLEEGDRVEVGELSYTVRHLPGHAPGHIGFFDFDNRIAFSGDVLFQNSIGR
                     TDLYLGNHSQLLETIREKMFDLDDDFIIIPGHGPHTTIGREKQTNPFLK"
     gene            complement(6766..7320)
                     /locus_tag="PXA33_001808"
     CDS             complement(6766..7320)
                     /locus_tag="PXA33_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HDL5121117.1"
                     /translation="MKQINVQRRKWLSLGGLVLGASLLPGQVMAALSTPAPAALRFRN
                     VNTGDTYTAKFGAGGLNKTDLSQLNYLMRDRHINQVKAIDPKLFVKLNQLQRRLGFHN
                     AEILVLSGYRSAQTNARLRRRSRGVASHSYHILGQAVDFQVSGVPLYKVKQAAESLNN
                     GGVGYYPRSNFVHVDTGPVRTWRG"
     gene            complement(7490..>8510)
                     /locus_tag="PXA33_001809"
     CDS             complement(7490..>8510)
                     /locus_tag="PXA33_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HDL5121118.1"
                     /translation="ANNWLYNLGSYVPKSPSSEQIFQWTNAVREGDSGQFVTDLVPRN
                     HLYRDTAQRVLAMAGGSSAPKAKKGKAVTEVAGGGNGASFYKLALNAQRLRIIPSFNN
                     GIFVNIPSYQLYYFRDGNLALQSKVIVGREDRRTPVMYSKLSNVVVNPPWNVPTSIMT
                     KDIIPKLARDPGYADRASFEILDSSGNKINPRSVNWSQYLNAKSSPYRIRQKAGDDSA
                     LGRYKFNMPSSDAIYLHDTPNRGLFGKTGRALSSGCVRVEKSDELATILLKEAGWGSD
                     KKNQVLGSKKTTSAQIRSDNPVYLYYVTAWVEGGKVQTLPDIYNFDAKIPQHSVNWGK
                     LKSII"
BASE COUNT         2596 a         1786 c         1564 g         2564 t
ORIGIN      
        1 aaaagttagc tcataattgt cgccatcacg actataggcg atatagcgag ggaatttttc
       61 accttcaaat tttttcaccg taatgtcttt tttatcactg gttctaaact tgtaggtaat
      121 ttcttggtag gttttgccgc ctacttttac ctctttttgg gctttgcgta gctgcacatt
      181 cggtgttggg taaagttttt taccgttagt agtttgaaaa ctgctcggta atttatcgta
      241 atagctcaac tgaaaggctg tggtgaacaa atcataagtt ggcatagtca gcgcttcttc
      301 ttttagtggc tctttcactt tgccatattt taccgtttta gaatcaattt ctgccatcgc
      361 ataagttttg ccgttacggg tatctcggta gctcaacata tgaaattgtt cgtttttttc
      421 tgttcccttc gcagaaaaga caatattata taaaggcaca ttaatttttg cctgaatatt
      481 atattggcca ccaccgttat taaagttaat gtaggctggc attaaataat ttgagctgta
      541 tttcaactta aaattctccg cactggcgtt tgaaatcaag cccaaagtga ccacgattac
      601 gctcgctaac ttctgccaaa ttctcatcta tttctcctct aaagatatac tttcgaacaa
      661 ccttgaaatt gcattgttat ctcgtaattg ttccgctttc tccacttgta cccgtgttaa
      721 atgtggcgaa aaataattaa taaaatcata catatagtta cgaagaaaag ttcctcgttt
      781 aaaggcgatt tgcgtcatac tcggttgaaa caggtgactt gcattgatag cgactaagtc
      841 tttatccgtt tccgtatgtg ccattgatgc cataatacca acccctaagc ctaagcgtac
      901 ataagttttg attacatcag catctgtcgc cgtaaatacg atattaggca aaataccttc
      961 cttattgaag gcatgatcta aatccgattg ccctgtaaaa ccaaaagtat aggttatcaa
     1021 atcatatcta cctaattgtt cgacagtcaa atttttactt tggttcacaa aagccgcaag
     1081 aggatgctct ggtttgacaa taactgaacg attccaccaa taacacggca ataaaaccgc
     1141 ttcatcaaat aaatattgag cttctgtggt aatggctaaa tccacttctc cagccaccaa
     1201 agcatcataa atttgattcg gggagccttg gtgcaaatgc aaacttactt caggatagcg
     1261 agccttaaac ttctcaatta cactcggtag catataacgg gcttgggtat tggtggtcgc
     1321 aatgcgtaag atgcctttgt tgggtcgagt ttgttcttca gcaaccgatt taatgctctg
     1381 ggcttttacc aataattcac gagcaatcgc cacaattttt tcaccagcag gcgttagccc
     1441 cttaacgtgc ttaccgttac gctcaaaaat attaattccc aactcacttt ccaacaaacg
     1501 aacttgtttg ctaatccccg gttgagaagt gtaaagcacc tctgcggcct cggtaatatt
     1561 caagttctgg tttactattt caacgatata acgtaactgt tgtagcttca taatgatttc
     1621 ccctctacaa tcgcaataat ctgttgcatc gtatgcactt cgtaagttgg cttaatatcc
     1681 gtatcattct ctttactaag caaatttaac caacaagtat caattccagc attattacct
     1741 cctaaaatat cagaagcgag ggtatcgcct accataagca ctttagtacg atcaaattcg
     1801 tccattaatg caaaagcgta atcaaacact tggcgatcag gctttgcggc accaatttgt
     1861 tctgatatca ctacaatatc aaaaaaatgt gaagtttgag tattatctaa acgtttttgc
     1921 tgcaaatcgg taaaaccatt ggtgataatg cccattttta ctttaccata taaagcattt
     1981 aacatatcca ttacaccttc taaaggctga ctgacgaatg ccatctccgc cattaactct
     2041 tgattaagtt gtagcggatc tacgcctgtt tgagcagata attttacaaa acgtctggtt
     2101 tgaatatctt gagcagtgat ctcattattt tggtaagcca cccaaagtgg tttatttacc
     2161 gcttgaaagt cttgataatc ttgttcacta aaatcaattt ggtaacgagc cagcatcgct
     2221 tttaaaccaa gataagaatt aaatgaaaac aaggtttcat cagcatcaaa taatacccat
     2281 tgatatttca taatattcct tttttaactt agataacaag cggtcaaatt tttctaattt
     2341 ttcgcatacc gctgataaag ccacgcttaa gcacaacact ctttcatttt ttgctgaatc
     2401 cactcaacaa aaaattgtac tttctgaact tgggctgatt gcgaaggata gacaacatag
     2461 aacgattttt catcaaacaa tgtggttggg aaaggctcta ttaaagtacc tttttcaatt
     2521 tcgtgctgtg ccaacaactg gttggcaatc gcaatgcctt gttggtgcat tgcagcttga
     2581 agcaccataa aagtatggct aaaaattgag ccagtttccg catctactat atcttctata
     2641 tgcaaatgtt caaccattct tttccactta ttatgggtgg aggaatgtaa taaatgctta
     2701 cctgctaaat ccgccggtaa atgaatggta ttttgcgata aatactcagg cgaagctaaa
     2761 ataacaatac gggattccgt caaacggata gattccagac cttgccaatc ccccttacca
     2821 taataaatag caacatctgt ttctttacct aataatccct catcttgatc aacaccctta
     2881 atacgtactt ccacttctgg atatttttcg tggaaatccg ccaaacgtgg gactaaccaa
     2941 tgcataccaa atgtttgtgg tacgctaata gtcagaattt gacgactact ttgctgccta
     3001 attttatctg ttgcgagagc aatttgttct aataatggct gaacttcata gaaatattgt
     3061 gcgcctaact cagttaattc cagcaaacgg ttacggcgat ggaacaggga agcacctaaa
     3121 aaatcttcta atagtttaat ttggtggctc actgcagctt gtgtgacgaa gagatcttct
     3181 gccgctttag taaaatttaa atggcgtgca gcagattcaa atgctttcaa tgcattgagt
     3241 ggtggaagtt ttttgttcat aatttttcaa tctaatagat tactaaacaa gcaactctat
     3301 tattttttct gatagtttaa ataaaaaaaa ttcgtttgat gcttgcaaat agaaataata
     3361 aaatatccgc atacttagat ggatagttta gaatttattt ctattcctaa attttaagta
     3421 tgatgttgtg tttgcatatt ggtctaggaa actagactag agtaacgaaa gttacttgtt
     3481 tcacttcctg tatattttga accgtattgg tttataaccg cccactttgg gcggtttttt
     3541 tctatgtaaa aaaacagcac aaaacctaga tcgcattcta tattattcgc tacctttatt
     3601 ctagcctttc tgctttttta ttctttcact ttaatctatt ataaaaagag gttttatagt
     3661 atgctaaagc ctatttttca tttgcaataa ttaccgagag ttcttttgag taaaccgaaa
     3721 ttaacccata accaacagcg tagaatccaa tctaaccatc ataagcgtat caacaaaaaa
     3781 gagattgaat ggcaagatga aatgttagga gaaatgcaac agggcatcgt cgttactcgc
     3841 cacgttaaac acgctgatgt tgaaaccgca gaggggcaaa ttttccgctg taatatccgc
     3901 cgtacattga aaaatgtagt ggtgggcgat caggtttctt ggcgtaaagg tagtgagcaa
     3961 ctacaaggga ttagtggggt cattgaagcc gtcttcccac gtaaaaatga acttacccgc
     4021 cccgattatt atgacggtat taaggtgatg gcatcgaaca tcgatcaaat cattattgta
     4081 tcagctgtgc tacctacact ttcgctgaat attatcgacc gttatttagt gatttgcgaa
     4141 acggcgaata ttcctgcctt aatcgtgcta aacaaaattg atttattaaa cgaaagcgaa
     4201 cggcaagcag ttaaagagca gctcaaaatc tatgaagaga ttggctatga aaccctatgt
     4261 ttatcggctg atacgggtga aaatatgcag gcgttacacc aatatcttgc taaaggtacg
     4321 tctatttttg tcggacaatc cggcgtgggg aaatccagcc taattaacca actgttacct
     4381 gaagtcaatg ctctcactgg ctcagtgagc gataactcag ggttaggtca gcacaccacc
     4441 actgcctctc gcttatacca tttaccgcaa ggcggacagc tgattgactc gccgggcatt
     4501 cgagaatttg gcttatggca tttagaacct gagcaaatca ccttaggcta tcgagaattt
     4561 caatcggttt taggcacgtg caaattccgt gattgcaaac acaaaaccga cccgggttgt
     4621 gccttaagac aagaggtcga aaatggcgaa atttctgcaa ttcggtttga aaattaccat
     4681 cgcttaattg aaagcctgtc tgaaaccaaa agccaacgcc atttcagaaa agaagaagta
     4741 taatgaatga atttatttac cctaacgccc gattaatcgc cggtgttgac gaagtcggac
     4801 gaggaccttt agtcggagcg gttgtgaccg cagccgtgat tttagatccg aataacccga
     4861 ttcagggctt aaccgattcc aaaaaattat ccgagaaaaa acgccaagct ttggcagaag
     4921 agattaaagc caaggctctg agctggtcgc taggacgtgc cgagccgaca gaaattgatg
     4981 agctgaatat tttaaatgcg acaatgttgg caatgcaacg tgcggttgcc ggcttgcata
     5041 ttcagccgga ttttgtgcta attgacggca atcgtccgcc aaaactgcca atgccggcac
     5101 aagcggtggt gaaaggagac agcttagtgg ctgaaatcag tgctgcttcg attattgcca
     5161 aagtcgcccg agatcaagaa atggacgaat tagaccaaca atttccgcaa tacggcttcg
     5221 cacagcacaa aggctacccg actaaactcc attttgaaaa attagcagaa tttggggcaa
     5281 caccttatca tcgtaagagt tttgcccctg taagaaaagt attaggttta atcgattaga
     5341 aggaaaacac aatgactaca gagtaccaaa aaacgatttt caccaaaatc atcaataaag
     5401 aaattccggc aaatattgtc tatcaagatg aacttgtgac cgcatttcgt gatattgctc
     5461 cacaagcacc cacgcatatt ttgattattc cgaataaatt tattccaacc gtcaatcacg
     5521 tgagtaatga agatgaatta gccctagggc gtttatttac ggtggcggca aaattagccc
     5581 aagaagaagg cattgccgaa gaaggctatc gcttagtgat gaattgtaat aaaaacggcg
     5641 ggcaggaagt gttccatatt catatgcact tgttaggcgg acaaaaacta ggacctttag
     5701 tggcaaaata actatgcgta tttcctactt tgcatttgca attttttcga tttttttgac
     5761 cgcttgtggt agcaaacctg tcagctattt accaaccggc tcagagccta ttgtcaatat
     5821 tgagtctgac attgctgaaa agatagagct ggaaacgaat agcaagcaat ttagcaccac
     5881 aaatttaact gaatttccgc ttaatcttac ttataaatta ttttggtata ataaagatgg
     5941 ggtaacacaa tcccataacg gtatatcaga tagtatcggc tggcaacatc tatggttgca
     6001 acctaagcaa aaacagacca ttgcactcat taaaccgacg gaagaaagcc ataattaccg
     6061 cctctattta cgaggcgtaa aatagcaaaa aagcagagcc aactgctctg cttctttatt
     6121 tatttcaaaa acgggttggt ctgtttttcc cgcccgatag ttgtgtgtgg accatgtccc
     6181 ggtatgatga tgaaatcatc atctaaatca aacatttttt ccctgatcgt ttccaataac
     6241 tggctatggt tgcctaaata gagatctgtt cgacctatgc tattttggaa taaaacatcg
     6301 ccactaaaag caatacgatt gtcaaaatca aaaaaaccga tatgacccgg agcatgaccc
     6361 ggcaaatgtc gaacggtata gctcagctcg ccgacttcga cccgatcacc ttcctccaac
     6421 caatgatccg gcaaaaagcc atttactggt ggtaatccaa attgttggca catttccggc
     6481 aaacgttcaa ataaaggttt atctgcaaga tgcgagccga aaacctctac ttgaaaatgc
     6541 tctctcaact tttccaccgc cataatatga tctaaatgac cgtgcgtaat caataatttc
     6601 tgaaccttaa gctgctgaga ttcaacaaat tgaataatgc cttcggcatt atcgccgcca
     6661 tcaataatag ccgcattctt attttcgtcc caaatcaccg tgcaattttg ttgaaatgga
     6721 ctaacaggaa taatttgcaa attaaacata aaataagacc gcttgttagc ctctccaagt
     6781 gcgaactggg ccagtatcta cgtgaacaaa gttactacga ggataataac ctacgccacc
     6841 attattcaag ctctcagccg cttgttttac tttatataac ggcacacctg atacttggaa
     6901 atctactgct tgcccaagaa tatggtaact atggctggca acaccacggc ttctacgacg
     6961 aagtcttgca tttgtttgtg cggagcgata gccacttaat actaggattt ccgcattatg
     7021 gaaacctaaa cggcgttgta attgattcaa tttgacaaat aattttggat caattgcttt
     7081 aacttgattg atatgacgat cccgcattaa atagtttaac tggcttaaat cggttttatt
     7141 taatccacct gcaccaaatt tagcagtgta agtatcgcct gtattcacat tacgaaaacg
     7201 taacgcagct ggggcaggag tagaaagcgc tgccattact tgacccggca gaaggctagc
     7261 ccctaaaaca agtccgccga gtgatagcca cttacggcgt tgaacattaa tttgtttcat
     7321 tttttcctca taggttagct aatgatttat agaaaatcac tcacaaatga gcgaatttct
     7381 atacaccata cttaaagtaa aaatttacaa tcgtaaattt atgtaagcta tattttcaaa
     7441 aagttccaag aagatgatat cttcttggaa tttatcagaa atagcacgat taaataatgc
     7501 tctttaattt tccccaattc acagaatgtt gtgggatttt ggcatcaaag ttataaatgt
     7561 ccggaagggt ttgcactttt ccaccttcaa cccaagccgt cacataatag agataaaccg
     7621 ggttatctga acgaatctga gccgaggtcg tcttcttact gcctaagact tgattctttt
     7681 tatcagatcc ccaacccgct tcttttaata aaatggttgc caattcatca gatttttcta
     7741 cacggacaca accagaactt aacgcacgcc ccgttttacc aaataatcct ctatttggcg
     7801 tatcatgcaa ataaattgca tccgaacttg gcatattaaa tttataacgt cctaaagcgc
     7861 tatcatcacc tgctttctgg cgaatacggt atggtgaact tttagcattt aaatattgtg
     7921 accaatttac gctacgcgga ttaattttat tgccactact atcaagaatt tcgaaactag
     7981 cacgatctgc ataacctggg tcacgcgcta gtttaggaat aatatcttta gtcataatac
     8041 tagtcggtac attccaaggt ggattcacta ccacattgct cagcttacta tacattaccg
     8101 gtgtacggcg atcttctcgc ccaacaatca ctttagattg aagagctaaa ttaccatcac
     8161 ggaaataata taattgatag cttgggatat tcacaaaaat tccattgtta aaacttggaa
     8221 taatacgtaa acgttgagca ttgagcgcta atttatagaa agaagcccca ttaccgccac
     8281 cagctacctc cgtaacagct ttaccttttt tggctttagg tgctgaagag cctcctgcca
     8341 ttgccaatac tctctgtgcc gtatcacgat ataaatggtt tcttggaaca aggtctgtta
     8401 caaactgccc agaatcgcct tccctcacgg cattagtcca ttggaagatt tgctctgaag
     8461 agggcgattt tggtacataa ctgcctaagt tatataacca attatttgca
//
LOCUS       DANLRK010000110         8461 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.110, whole genome shotgun sequence.
ACCESSION   DANLRK010000110 DANLRK010000000
VERSION     DANLRK010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8461
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.110"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            30..1835
                     /locus_tag="PXA33_001810"
     CDS             30..1835
                     /locus_tag="PXA33_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HDL5121119.1"
                     /translation="MLSKLQRFTLMIKSTIIKHPLEILFVTYVTILLINNVSDYELIK
                     RFQNLIALFPICFILLYLIRDTKAYWLFAPIPIAIVFFFGQINLTLLEDSRYWATIFC
                     VFLCLISQYWYKNNHYFVAGTTNKLVNIVFATVLSGIIFATLSAITIAIIALFDLNKQ
                     NNFHIFEKFGIFSSFWAFPVLFLTLEQQTTNDSRYLNRISEMLLNWILSPALIIYTAI
                     IYAYVIFIAAKGQMPDGVVANVALPYLAFGLAIQAVQLLLKNAKWQWFYSHFTYLALL
                     PLTLIWYAIYIRFSHYGLTEARIYLVIGTITLSICYTLLLFKQLAQYRLFSAVSIAII
                     LISVFILDPKTVALKDQTKRLDNYLLKHKLLDVNNKISTKAVLKYQESLNENRDEAEY
                     FYSMLHYVARGMPAAQFKEKYGVNSSYDLMSEIRERNTDPRYFEATNSELVRLTKADM
                     ANAREYITFSSQLYRPNSFSHQKIENKLCQKLLSQGYYFEGNILNIEYQYSETAQDCS
                     SITQDPTEFVIYFKGLGTTIEFNINDILKKIFAKHQLSITQQHKNEVLEKIKGDLLKM
                     DLGNSVLGLKHIELRFEDNIGYIVESISTQYIMLK"
     gene            complement(1878..4907)
                     /locus_tag="PXA33_001811"
     CDS             complement(1878..4907)
                     /locus_tag="PXA33_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4981 domain-containing protein"
                     /protein_id="HDL5121120.1"
                     /translation="MSLPNYYQDPSVLHLNTTLHHAYFIPFAKGQTPNQLEREQSELF
                     TLLNGDWKFNFYPSPHDLPEDFLNTKFTHQIPVPSNWQTQGFDAHQYTNINYPIPFDP
                     PYVPHQNPCGVYQREITFNPKPNKRYLLNFEGVDSCLFVYVNGEFVGYGQISHSTNEF
                     DITKQLKQGKNSLVVVVLKWCDGSYLEDQDKFRMSGIFRDVYILEREQNYLQDFFIRY
                     QLNADLSKAELKVETQFSTYTEAVHFQLFDPENRLVCEQTSGDFCQEICNLCLWNAED
                     PTLYTLCLSYQNEIIEQKIGFRQIRVENGVMLFNNAPIKFKGVNRHDSDPKTGYSISR
                     EQALVDLRLMKAHNINAIRTAHYPNAPWFSELCDQLGFYLIAESDLESHGSSAVYIES
                     PENSIFLNVPNPNKQEEIRQQAVDNFCYFARDPLFKQAILDRNIANVERDKNRTSVII
                     WSLGNESGFGENFEVAARWVKQRDPSRLTHYEGSIYQHSAHQNDLSNLDLYSEMYPST
                     ERLEEYFSDSANHKPFILCEYSHAMGNSSGDMEDYWQTFQRYSGACGGFVWEWCDHAP
                     LLPNSELSAEQKTEKYGYGGDFGETLHDGNFCMDGLVSQQRVPHSNLLEVKNVNRPVR
                     AELKAGKIWLKNQLDFSDLADYLTVHYCFSENGETTLQGQCLVNCQPNQTACLPLTLP
                     EPNGKLQLLTLDYSLNESTALLEKGHHLGFDQITLHQAMVTPALNPRPQAVIFVQDFA
                     NEWRIQQGDYRYTFDKLKGIFSRIEKNGEAVIEQPLDFNIWRAPTDNDRLIREFWKKA
                     GYDNCYTRAYHTELMQDKNGVNITAECGIVATAQSRILTLKVNYHLAPNGEMTIQVQA
                     TRPAHLPFLPRFGLRFFLNKRNNQVSYFGYGEQESYIDKYHLAKLGAYQTSARENHTD
                     YLKPQENGSHFGTHYVQANGITVTSPQPFSFNLSPYTQEELTTKSHSYDLVESPSTVL
                     CVDYKMSGIGSNSCGPNLKPQYRLNETNFEWLLKLSF"
     gene            5085..5942
                     /gene="menB"
                     /locus_tag="PXA33_001812"
     CDS             5085..5942
                     /gene="menB"
                     /locus_tag="PXA33_001812"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005723289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HDL5121121.1"
                     /translation="MLYPSEEFLYAPVEWVDHSEGYTDIRYHKSKDGIAKITINRPEV
                     RNAFRPQTVKEMIEAFSDARFDEKIGVIVLTGEGEYAFCAGGDQKVRGDYGGYKDESG
                     VHHLNVLDFQRDIRTCPKPVVAMVAGYAIGGGHVLHMLCDLTIAADNAKFGQTGPKVG
                     SFDGGWGASYMARLVGQKKAREIWFLCRQYNAQEALDMGLINTVVPYADLEKETVRWC
                     REMLQNSPIALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNEKREP
                     DFSKFRRNP"
     gene            6025..8427
                     /gene="mrcB"
                     /locus_tag="PXA33_001813"
     CDS             6025..8427
                     /gene="mrcB"
                     /locus_tag="PXA33_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1B"
                     /protein_id="HDL5121122.1"
                     /translation="MSDVELNEQSEQEPEQKPKKRNYFLSTLFKIGVIGACSMAFYGI
                     YLDGKIRSKMDGQIWELPAEVYARIESIKIEDNLTLEQTKQALLDNGYRQVSQVATPG
                     DFKIEENQLVLLRRAFPFPETPESQRVLRLTFKQDKLTYIEDLVQGKLIEEFRLDPKL
                     LALLHSDSDEERQALRLQQYPRLLIESLILTEDRRFYQHDGISPIGIARALITNYQAG
                     RTVQGGSTLTQQLVKNLFLSNERSLVRKVNEALMSVILDFRYDKNRILETYLNEIYLG
                     QNGSYQIHGFALASQFYFGRPIQEISLDQIALLVGMVKGPSLYNPWRNPQGAVERRNV
                     VLRLLLDHQVINQELYALLSSRPLNIREKGTIYREQPAFMQALTIDLKNELGEIKSAQ
                     LSGTKIFTTLDRKQQRSAEQAVITGLEDLESKNKKVSDLQSAIVVAEYRTGKVRAIVG
                     DRLTQFAGFNRAIQSKRQIGSLVKPSVYAIALAQPELFRLNTPIQNKQISIKVGSTYW
                     TPRNYDRSFSGSVMLMDALVRSLNIPTVNIGMKVGLKKVIAKQKEMGWDNAEIPPYPS
                     TLLGSYSISPYDVTRSYQVIANEGLKTPLTTIEAIISHNGEMFYQRDSGKVSEQVLPP
                     EAAVQTMYAMQQVVERGTARSLQNDFAKLKLAGKTGTTNNARDTWFVGIDGEHVTTVW
                     LGKDNNGDTNLTGSSGALYVYKQYLERALPQAYRLPKLNTMQWVGINSYGSWNCDSGR
                     QIPVWRDKGQRYCTSAGNVVATEKPSVWDALSLKKENIEAKAEEAEGKQPIPTEEVAP
                     IE"
BASE COUNT         2493 a         1690 c         1824 g         2454 t
ORIGIN      
        1 atattctttt cataggcagg agatctatta tgctaagtaa gctacaacgc tttaccttaa
       61 tgataaaaag caccatcatt aagcatccac tggaaatttt attcgttact tacgtcacga
      121 ttttacttat caataatgtg agcgactacg aattaatcaa acgctttcaa aatctaattg
      181 ctctttttcc tatttgcttt attttgcttt acctgatacg cgataccaaa gcttattggc
      241 tattcgctcc gattccgata gcaatcgtct ttttctttgg tcaaattaat ttaacattat
      301 tggaagattc tcgttattgg gctaccatat tttgtgtatt cctttgtttg atcagtcaat
      361 attggtacaa aaacaaccat tattttgtag ccggaacaac taataaatta gttaacattg
      421 tctttgctac agttttatct ggcattattt ttgcaacatt aagcgctatt accatagcca
      481 ttattgcttt atttgattta aataaacaaa acaactttca tatttttgaa aaatttggca
      541 ttttttctag tttttgggca ttccctgttt tatttttaac tcttgaacaa cagactacca
      601 atgattctag atatctaaac cgcattagtg aaatgttact aaactggatt ttgtctccgg
      661 cattaattat ctatactgca attatttatg cttatgtaat ttttattgcg gctaaggggc
      721 aaatgccaga tggggttgtt gcaaacgtag cgcttcccta tttagctttc ggcctagcta
      781 tacaagcggt gcaattatta ctaaaaaatg caaaatggca gtggttttat tctcacttta
      841 cctatttagc acttttgccg ttaacattaa tttggtatgc catctatatt cgtttcagcc
      901 attatggctt aactgaagcc cgtatttatt tagtgattgg cacaataacc ttatctattt
      961 gctatacctt attactattt aaacagttgg cgcaatatcg tctcttttct gcagtgtcga
     1021 tagcaattat tttaatttca gtgtttattt tagatccgaa aacagttgcg cttaaagatc
     1081 aaaccaagcg gttggacaac tatttgctaa aacacaaatt attagatgta aataacaaaa
     1141 tcagcacaaa agctgtactt aaataccaag aatcattaaa tgaaaaccga gatgaagccg
     1201 aatattttta ttcaatgctc cactatgttg ccagaggaat gcctgctgca caatttaaag
     1261 agaaatatgg cgtaaattca agctacgatt taatgagtga aataagagaa agaaatactg
     1321 acccacgtta ttttgaagct actaattctg agctggttcg cttaactaaa gcagatatgg
     1381 caaatgctcg tgaatatatt acatttagta gccaattata tcggcctaat tcattttccc
     1441 atcaaaaaat agaaaataag ctatgccaaa aactcctttc tcaaggttac tattttgagg
     1501 gaaatatatt aaatattgag tatcaatact cagaaacggc tcaagactgc tcttcaataa
     1561 cacaagatcc tactgaattt gttatctact ttaaaggact tgggactact attgaattta
     1621 acattaacga tattttgaaa aaaatctttg ctaaacatca gttatctatt acccagcaac
     1681 acaaaaatga ggttttagaa aagattaaag gcgatttact caaaatggat ttaggaaatt
     1741 cagtacttgg gcttaagcat attgagttaa gatttgaaga caatatcggc tatatagtag
     1801 aaagtattag tacacaatat attatgctga aataaaagga gatgctcgtt caattaaatt
     1861 tgaacgagca tttttcgtta aaaactcaat ttaagcagcc actcaaaatt agtctcattt
     1921 aaccgatatt gcggtttcaa attcgggccg caagagtttg agccaatgcc gctcattttg
     1981 taatcgacac acagcacggt ggaagggctt tctaccaaat cataactgtg gcttttagtg
     2041 gttagctctt cctgtgtgta aggcgaaaga ttaaaactaa acggctgtgg agaagtaaca
     2101 gttatcccat ttgcttgcac ataatgcgta ccgaaatggc tgccgttttc ctgcggtttg
     2161 agataatcgg tatgattttc tcgggcgctg gtttgataag ctccgagttt cgccaaatgg
     2221 tatttgtcga tataactctc ctgctcgcca tagccaaaat agctcacttg gttattccgt
     2281 ttattcaaga aaaaccgcaa gccaaagcga ggtaaaaacg gcaaatgagc ggggcgggtt
     2341 gcctgaacct gaatcgtcat ttcgccattc ggggcaagat gataattcac tttcagggtt
     2401 aaaatccgac tttgagcggt tgccacaatg ccacattcgg cagtaatgtt tacgccgttt
     2461 ttatcttgca tcagttcggt atgatacgcc ctggtgtaac agttatcgta gccggctttt
     2521 ttccaaaact cacgaattaa gcggtcgtta tcggttgggg ctcgccaaat attgaaatcc
     2581 aacggttgct caattactgc ctcgccgttt ttctcaatcc ggctgaaaat gcctttgagt
     2641 ttatcgaaag tgtagcgata gtcgccctgt tgaatccgcc attcatttgc aaaatcttgc
     2701 acaaaaatga ccgcttgcgg gcgtggatta agtgctggag tgaccatcgc ttgatgcagc
     2761 gtgatttggt caaagcctaa gtgatgccct ttttccaaca gagccgtgct ttcatttaaa
     2821 gaataatcaa gggttaaaag ttgcaatttg ccgtttggtt cgggcagcgt tagcggcaaa
     2881 caagcggtct gatttggctg acaatttacc aaacattgtc cctgcagggt ggtttcgccg
     2941 ttttcgctga aacagtaatg cacggtcaga taatcagcta agtcgctgaa atccaactga
     3001 tttttcagcc aaattttgcc cgcttttaac tcagctcgca ccgggcgatt cacatttttc
     3061 acttctaaca aattagagtg tggcacacgc tgttgggaaa ccaagccgtc catacagaaa
     3121 ttgccatcgt gcaaggtttc gccgaaatca ccgccatagc cgtatttttc ggtcttctgt
     3181 tcggcactta gctcagagtt ggggagcaga ggagcatggt cgcaccattc ccacacaaag
     3241 ccaccgcacg caccgctgta gcgttggaag gtttgccaat aatcttccat atcaccactc
     3301 gaattgccca tcgcgtgaga atattcacac aaaataaaag gtttgtggtt ggcagagtcg
     3361 gaaaaatatt cctctaaccg ctcggtgctt gggtacatct cgctgtataa atcgaggttt
     3421 gaaagatcgt tttgatgtgc cgaatgttgg taaatcgaac cttcgtagtg agttaaacgg
     3481 cttggatccc gttgttttac ccaacgggct gcgacttcaa aattttcacc aaagccggat
     3541 tcgttaccaa gtgaccaaat aatgaccgaa gtgcggtttt tgtctcgctc cacattggca
     3601 atgttgcgat ctaaaatcgc ctgtttgaac agcggatctc ttgcaaaata acagaaatta
     3661 tcgaccgctt gttggcgaat ttcttcctgt ttgttcgggt ttggcacatt caggaaaatg
     3721 ctgttttccg gactttcaat atacactgca ctggagccgt gagattcaag atcactttcg
     3781 gcaatcagat aaaaaccgag ctgatcgcac aattcactaa accacggagc gttcgggtaa
     3841 tgggcggtgc gaatagcgtt gatgttatgg gctttcatta agcgtaaatc caccaacgct
     3901 tgctcacggc taatgctgta gccggttttc gggtcggaat cgtggcggtt taccccttta
     3961 aatttaatcg gggcgttgtt gaatagcatc acgccatttt ccacacgaat ctgacgaaag
     4021 ccgatttttt gctcaataat ttcgttttgg tagctcaagc atagcgtgta gagtgtcgga
     4081 tcttccgcat tccacaaaca caaattgcaa atttcttggc aaaaatcacc gcttgtttgc
     4141 tcgcagacta agcggttttc agggtcgaaa agttgaaaat gcacagcttc ggtgtaggtg
     4201 gaaaattgtg tttccacctt gagttctgcc tttgaaagat cagcgtttaa ctggtagcgg
     4261 ataaagaaat cttgcaggta gttttgctca cgttccaaaa tataaacatc acggaaaatg
     4321 cctgacattc ggaatttgtc ttgatcttcc aaataactgc cgtcacacca ttttagcacc
     4381 accacgacga ggctgttttt gccctgttta agctgtttgg tgatgtcaaa ttcattggtg
     4441 ctgtggctga tttgcccgta gccgacaaat tcgccgttta cataaacaaa caggcacgaa
     4501 tctacgccct caaaatttag caaatagcgt ttgttaggct tggggttaaa cgtgatttca
     4561 cgctgataca ccccgcaagg gttttggtgt ggcacataag gcggatcaaa cggaatcggg
     4621 tagttgatgt tagtgtattg atgggcatca aagccttgag tttgccagtt agacggcacg
     4681 gggatttggt gggtaaattt ggtgttgagg aaatcctccg gcagatcgtg cggacttgga
     4741 tagaaattaa acttccaatc gccatttagc aaagtaaata actcagattg ttcccgttcc
     4801 aactgattcg gcgtttgccc tttagcaaaa ggaataaaat aagcgtggtg tagcgtggta
     4861 ttaaggtgca acacactcgg gtcttggtag taattcggta acgacataaa agccctatga
     4921 tcaaaaagat ataaatacga atacacttta attctcttat aaaatagggc aatcaactat
     4981 gtgaaaaatt gtgaggcgga tcacaaaata atttcctcac ctttttcctt cgattccgtt
     5041 ataatcacaa gaatttttta tgtcattttt caaggaattt accaatgtta tatccaagcg
     5101 aagaattttt atatgcaccg gttgagtggg ttgatcacag cgaaggttac accgatattc
     5161 gttaccataa atcaaaagac ggtatcgcta aaatcaccat taatcgtccg gaagtgcgta
     5221 atgctttccg tccgcaaacg gtaaaagaga tgattgaagc cttttctgat gcccgttttg
     5281 atgaaaaaat cggcgtcatc gtattaaccg gtgaaggcga atatgcgttc tgtgccggcg
     5341 gcgaccaaaa agtgcgtggc gattacggcg gctacaaaga tgaaagcggt gtgcatcact
     5401 taaatgtgtt ggatttccaa cgtgatattc gtacctgccc gaaaccggtg gtggcaatgg
     5461 ttgccggcta tgcgattggt ggtggacacg ttttacatat gttatgtgat ttaaccattg
     5521 ccgctgacaa tgcaaaattc ggtcaaaccg gcccgaaagt ggggtcattt gacggcggtt
     5581 ggggtgcaag ctatatggca cgtttagtcg gtcagaaaaa agcgcgtgaa atttggttct
     5641 tatgccgtca atataatgca caagaagcgt tagatatggg cttaatcaat accgttgtgc
     5701 cttatgctga tttagaaaaa gagactgtgc gttggtgtcg cgaaatgcta caaaacagcc
     5761 cgattgcctt acgttgctta aaagcagcat tgaatgcaga ttgtgacggt caggctggcc
     5821 tgcaagagct tgctggtaat gcaacaatgt tgttctatat gacagaagaa gggcaagaag
     5881 gacgcaatgc gttcaacgag aaacgtgagc cggacttcag caaattccgc cgcaatccat
     5941 aattgacggc aaatacaagc ggttcgattt tgcaaaaaat ttgcaaaaat cgaccgctta
     6001 ccttttctaa tttaagttaa ttttatgtca gatgttgagt taaacgagca gagcgagcaa
     6061 gagcctgagc aaaaaccgaa aaagcgaaac tatttccttt caaccttatt caaaatcggg
     6121 gtgataggag cttgcagtat ggctttttac ggcatttatt tagacggtaa aatccgtagc
     6181 aaaatggatg ggcagatatg ggaattacca gctgaggtgt atgctcgtat tgaaagcatt
     6241 aaaattgaag acaacttaac gcttgagcaa accaaacaag cgttgctgga taacggctat
     6301 cgtcaagttt ctcaagttgc gacaccaggc gatttcaaaa ttgaagaaaa tcagctggtg
     6361 ttgctacgcc gagctttccc gtttccagaa acaccggaat cgcaacgagt gcttcgttta
     6421 actttcaaac aagataaatt aacttatatt gaagatttgg tgcagggtaa gttgatcgag
     6481 gagttccgct tggatccgaa attactggcg ttactgcatt ccgatagcga tgaagaacgt
     6541 caagcattac gcttacaaca ataccctcgc ttgttaattg agtcactgat tttaaccgaa
     6601 gatcgccgct tttatcagca cgatgggatt agcccaatcg gtattgcccg tgctttaatc
     6661 actaactacc aagctggcag aacagtgcag ggtggcagta ccttaacgca acagttagtg
     6721 aaaaacttgt tcttaagtaa tgaaagatcg ttggtgcgta aggtaaatga agcgttaatg
     6781 tcggtgattt tagacttccg ttacgataaa aaccgcattt tagaaaccta tctcaacgaa
     6841 atttatttag ggcagaacgg tagctaccaa attcacggtt ttgcattggc aagccaattc
     6901 tattttggtc gtccgatcca agagattagc ttagaccaaa ttgcgttatt ggtgggaatg
     6961 gtaaaaggtc catcactgta taacccgtgg cgaaatccgc aaggtgcggt agagcgacgt
     7021 aatgtggtgt tgcgattatt gctggatcat caagtgatca atcaagaact ttacgctctg
     7081 ttaagctccc gcccgcttaa tattcgtgaa aaaggcacga tttaccgtga gcaacctgcc
     7141 tttatgcagg cattaaccat tgatttgaaa aatgagttag gcgaaattaa gtcagctcaa
     7201 ctttccggta ctaaaatttt tacgaccctt gaccgtaaac agcaacgttc agccgaacaa
     7261 gcagtgatta ccggtttgga agatctggaa agtaaaaata aaaaagtcag cgatttgcaa
     7321 tcggctattg tcgtggcgga ataccgcaca ggtaaagtga gggcgatagt aggagaccgc
     7381 ttaacccaat ttgccggctt taaccgtgcg attcagtcaa aacgccaaat cggttcttta
     7441 gtcaaaccat cggtttatgc gattgccttg gcacagcctg aattattccg tttaaataca
     7501 ccgatccaaa ataaacaaat ttcaattaag gttggttcaa cctactggac accccgtaac
     7561 tacgaccgca gttttagtgg ctcggtgatg ttaatggacg ccttagtccg ctctctgaat
     7621 attccaacgg tgaatattgg tatgaaagtt ggcttaaaaa aagtcattgc caaacaaaaa
     7681 gagatgggct gggataatgc ggaaattccg ccttacccat caaccttgct aggttcatat
     7741 tcgatctcgc cttacgatgt aacccgctct tatcaagtaa ttgccaacga agggctgaaa
     7801 acaccattaa ccaccattga ggcgattatt tcgcacaatg gcgaaatgtt ctatcagcga
     7861 gatagcggca aagtgagcga gcaggtgctt ccaccggaag ctgcggttca aacaatgtat
     7921 gcgatgcaac aggttgtgga acgaggtacg gctcgtagct tacaaaacga ttttgccaaa
     7981 ctgaaattag ccggcaaaac cggtacaacc aataacgctc gagatacttg gtttgtggga
     8041 attgatggcg aacacgtcac aaccgtctgg ctgggtaaag ataacaatgg ggataccaac
     8101 ctcaccggtt caagtggtgc attgtatgtg tataagcaat atcttgagag agccttgcct
     8161 caggcgtata gattaccgaa acttaatacg atgcaatggg tggggattaa tagctatggt
     8221 agctggaatt gcgatagtgg cagacaaatt ccagtttggc gagataaagg gcaacgttat
     8281 tgtaccagtg caggtaatgt agtggctaca gaaaaaccaa gtgtttggga tgcactttcg
     8341 ctgaaaaaag aaaatatcga ggctaaagca gaagaagctg aagggaagca accaatccct
     8401 actgaagaag ttgcaccaat tgaataaaaa taaatttgat agccattatc attcaagata
     8461 c
//
LOCUS       DANLRK010000111         8344 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.111, whole genome shotgun sequence.
ACCESSION   DANLRK010000111 DANLRK010000000
VERSION     DANLRK010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8344
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.111"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(126..764)
                     /locus_tag="PXA33_001814"
     CDS             complement(126..764)
                     /locus_tag="PXA33_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDL5121123.1"
                     /translation="MELKHTIEQRKTIKAFNSTIKISRTELEEMVALSQLAPSKANLQ
                     PWRFVIIDVEILKAALSKNVAFNGPPCETASAIVVILADLQYEKLLGAIVDQSVETGC
                     LHKEFRERKFDFLLNLHNQATQAEIRDQVLIDTSLAAMQLMLIAKDKGYDTHAIGVFE
                     RPAVLKLLNIDAERYLPVMLLAIGKAANPALPSCRLPLEYTVSWNDGQGFKI"
     gene            complement(906..1700)
                     /locus_tag="PXA33_001815"
     CDS             complement(906..1700)
                     /locus_tag="PXA33_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL5121124.1"
                     /translation="MALLEVNHLNKRFVERSSLFHKQDFYAVKEVSFTLNRKETLAII
                     GENGAGKSTLAKMVAGMVGPTSGELIFRGTPLSFGDYKYRAKHIRMMFQDPNDAFDPN
                     YNIGQILDAPLRLATDLNEEERNERIFNTLKLVGMYPEQALVPISEASSGQKQRIAFA
                     RALILEPEIIIADDTINSLDFSVKTQLINLMLSVQQRQGIAYIYVGQNLGLIKHIADK
                     IMVMQSGEVVEYGLTKEVLLNPQHQITKRLIESHFGKHLSSYAWAS"
     gene            complement(1801..2877)
                     /gene="fbaA"
                     /locus_tag="PXA33_001816"
     CDS             complement(1801..2877)
                     /gene="fbaA"
                     /locus_tag="PXA33_001816"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014990940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HDL5121125.1"
                     /translation="MSLLNIVKPGVVTGDDVQKVFAYAKEHNFAIPAVNCVGSDSVNA
                     VLETAARVKSPVIVQFSNGGAQFYAGKGIKPASGARADVLGAIAGAKHVHQLAEEYGV
                     PVILHTDHAAKNLLPWIDGLLEAGEKHFAETGRPLFSSHMIDLSEEPIEENMEICREY
                     LARMDKIGMTLEIEIGVTGGEEDGVDNSDVDESKLYTQPSEVLYVYDQLSPISPRFTI
                     AAAFGNVHGVYKPGNVKLKPSILGASQEYVSKERGLPAKSLDFVFHGGSGSSREEIRE
                     AISYGAIKMNIDTDTQWAAWDGILQFYKANEAYLQGQLGNPTGPDAPNKKYYDPRVWL
                     RKMEESMSKRLEQSFEDLNCVNVL"
     gene            complement(2974..4149)
                     /locus_tag="PXA33_001817"
     CDS             complement(2974..4149)
                     /locus_tag="PXA33_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012072290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HDL5121126.1"
                     /translation="MSVIKMTDLDLAGKRLFIRADLNVPVKDGKVTSDARIVATIPTL
                     KLALQKGAKVMVTSHLGRPTEGVFEEENSLQPVVDYLNASDLGVPVRLVRDYLDGVEV
                     NENEIVVLENVRINKGEKKNDPELAKKYASLCDVFVMDAFGTAHRAEGSTYGVAEYAP
                     VACAGPLLAAELDALGKALKEPQRPMLAIVGGSKVSTKLTVLDSLSKIADQLIVGGGI
                     ANTFIAAEGHAVGKSLYEEDLIPEAKRLAQVTNIPVPVDVRVGTEFSETAPATEKAVS
                     EVRADESIFDIGEKSSEQLAEIIRNSKTILWNGPVGVFEFPNFRKGTEVISNAIAEAT
                     ANGAFSIAGGGDTLAAIDLFGIKDKISYISTGGGAFLEFVEGKVLPAVEILEKRANG"
     gene            complement(4404..5762)
                     /locus_tag="PXA33_001818"
     CDS             complement(4404..5762)
                     /locus_tag="PXA33_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HDL5121127.1"
                     /translation="MANQQQERQSWSNRLAYIMTVAGATVGFGATWRFPYLVGEHGGG
                     AYVFLFCIAMIVIGIPMILVENVIGRRMRVNAIDAFAGFANGKKIASGWKILGYMGLL
                     GAFGILAYYMVLGGWVLNYIGSLLTGTLDLSQPVTVGTTQQFFQQSIFNSPMTIIAYT
                     ALFVLVNYFILVKGIVDGIERSVKYLMPILFIFLIVMVVRNITLPGAAEGIAFYLMPD
                     FSKITPKLFVFVLGQVFFALSLGFGVLITLSSYLDKSENLVKTATITGVMNTLIAVLA
                     GFMIFPSLFSFGVAPNSGPTLVFQSLPIVFSNMWGGTVFAVIFFSLLVVAALTTSLTI
                     YEVIITALQEKAKISRQKAIFITLATIFLLGNIPSALSDNILQDVQVMGKSIFDAFDY
                     ISGNILFILTALGSAIFVGFVLKEEAKEELGAGKMFTTIWFNYVRFVIPVIILVIFFN
                     SL"
     gene            complement(5832..6659)
                     /locus_tag="PXA33_001819"
     CDS             complement(5832..6659)
                     /locus_tag="PXA33_001819"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HDL5121128.1"
                     /translation="MNHPKHRAFLKWAGGKYRLIPDIQTHLPKKACLVEPFVGAGSVF
                     LNTDFERYILADINPDLINLFNVVKADVEEYIAQTKQLFLHPQANTEAFYKARRAEFN
                     RSKDIFRRSVIFLYLNRFGYNGLCRYNQKKGYNVPFGRYKTHYFPEKELRFFAEKAQK
                     ATFVCADFEQVFELAKNQLTDYVIYCDPPYAPLAQETNFTQYAGGGFSLEQQGRLATL
                     ALEMKGLGVPVLISNHDTDFTRRIYQSADITQIQVQRSIGQKVGSRVKVGELFALFI"
     gene            complement(6673..7761)
                     /gene="aroB"
                     /locus_tag="PXA33_001820"
     CDS             complement(6673..7761)
                     /gene="aroB"
                     /locus_tag="PXA33_001820"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005611318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HDL5121129.1"
                     /translation="MLQVNVELKERRYPIIIGAGLLNQPASYSPLKSGDKVMIVSNPT
                     VATHYLSVVTNALKELGCHVDSVLIPDGEEYKTLESLNLIFTALLEKNHNRDTTLIAL
                     GGGVIGDVAGYAAASYQRGIRFIQIPTTLLAQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     VSVIVDTNTLHTLSKREVSAGLAEVIKYGAIFDVTFFEWLEKHIDDLVSLKQDELEYC
                     IQRCCQLKADVVARDETEKGDRALLNLGHTFGHAIEARMGYGVWLHGEAVSVGMLEAA
                     ELSRILGDLSPQEVARLEKLLARANLPTISPDGMEPAEYLPYMWRDKKVLAGQLRLIL
                     LKSLGEAYVSSQATESQVLASIERFTQR"
     gene            complement(7785..8306)
                     /gene="aroK"
                     /locus_tag="PXA33_001821"
     CDS             complement(7785..8306)
                     /gene="aroK"
                     /locus_tag="PXA33_001821"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005710312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HDL5121130.1"
                     /translation="MAEKRNIFLIGPMGAGKSTIGRQLAQTLGMEFMDSDSVIEERAG
                     ADIDWIFDVEGEEGFRKREERILNELTQNYGIVLSTGGGSILSKDNRNVLSARGIVIY
                     LETTVDKQFERTQRDKKRPLLQTEDPRKTLEELAKIRNPLYEEIADITIHTDEQSAKV
                     VANQIIDMIDNLQ"
BASE COUNT         2600 a         1832 c         1422 g         2490 t
ORIGIN      
        1 tatttgccct agtatttaat tccgaatgca tatatctcct ataaatagca atacaaaaat
       61 aaattacaac gctcaaattt taacaatttt ttgccaaaaa attaagatat ttaaccgctt
      121 gcaccttata ttttaaaacc ttgtccatcg ttccaagaaa ccgtatattc tagaggtaaa
      181 cggcaacttg gcaacgctgg gtttgctgct ttaccgatag ctagtagcat aacaggcaaa
      241 taacgttctg catctatatt caataatttt aatacagcag ggcgctcaaa cacgcctatc
      301 gcatgagtat catagccctt atctttggca attagcatta attgcattgc cgccaaacta
      361 gtatcaatta atacctgatc tctaatttct gcttgagttg cttggttatg aagatttagt
      421 aaaaaatcaa atttacgttc acgaaactct ttatgcaaac acccagtttc taccgattga
      481 tcaacaattg cgcctaataa tttttcatat tgcaaatctg ccaatatcac cacaatagca
      541 gacgctgttt cacaaggtgg gccattaaag gcaacatttt ttgataatgc tgctttaaga
      601 atttcaacat caataatgac aaaacgccaa ggttgcagat ttgcttttga gggagcaagc
      661 tgggataaag cgaccatttc ttctaattca gttctactaa tcttaattgt tgaattaaaa
      721 gctttgatag ttttacgctg ttcaatcgta tgtttcaatt ccatctcttt tctcctatct
      781 ctcaaacaat tgagatagtc tataatttaa agattaggaa tgtaataatt gaaaagagtt
      841 attctgcata tttattgaaa taggtcaaaa ttatcaagta atcatattag agcaggagta
      901 aagatttagc ttgcccaagc ataggaacta aggtgctttc caaaatgact ttcaattaaa
      961 cgtttcgtaa tttgatgttg cggattcagt aaaacctctt tcgtcaagcc atactctact
     1021 acttcgccac tttgcatcac cattatttta tcggcaatat gtttgatcag acctaaattt
     1081 tgccccacat aaatataagc aatcccttgt cgctgctgga cacttaacat taaattaatt
     1141 aattgtgttt tgactgaaaa atccagtgaa ttaatcgtat catccgcaat aataatttcc
     1201 ggttctaaaa ttaaagcacg agcaaacgca atacgctgct tttgcccact ggaagcctct
     1261 gaaataggaa cgagcgcttg ctcaggatac attcctacca attttagcgt attaaaaata
     1321 cgctcatttc tttcctcttc atttaaatct gtcgccaagc ggagcggagc atctaaaatt
     1381 tgtcctatat tataattagg atcaaaggca tcgttagggt cctgaaacat catacgaata
     1441 tgcttagctc ggtatttata atcgccaaat gaaagcggtg ttcctctaaa aatcagttct
     1501 ccagatgttg gcccaaccat accagcaacc attttagcca aggtagattt cccagctccg
     1561 ttttcaccaa taatcgctaa agtttccttg cgatttaggg taaaagaaac ttcttttacc
     1621 gcataaaaat cttgtttatg aaataggcta ctacgttcaa caaaacgctt attcagatga
     1681 ttaacttcaa gtaatgccat aataaattgg tgcttggaaa taaagtgata gtttaccgca
     1741 aaaaataaat aaaaaaagac cgcttataca agcggcctga tttatggaat tttttgcaaa
     1801 ttataaaacg ttcacacagt ttaagtcttc gaaagattgc tctaagcgtt tagacataga
     1861 ctcttccatt ttacgtaacc aaacacgtgg atcgtagtat tttttgtttg gtgcatcagg
     1921 gcctgtcggg ttacctaatt gaccttgtaa atatgcttcg tttgctttgt agaattgaag
     1981 aataccgtcc cacgcagccc attgggtgtc agtgtcaatg ttcattttga ttgcaccgta
     2041 gctgattgct tcacgaatct cttcacgtga agaaccagaa ccgccgtgga aaacgaagtc
     2101 taatgacttt gccggtaaac cacgctcttt agatacatac tcttgtgaag cacctaagat
     2161 tgatggtttt aattttacat tacccggctt gtaaacaccg tgtacgttac caaatgctgc
     2221 cgcaatcgtg aaacgtgggc tgattgggct taattggtcg taaacatata aaacttcaga
     2281 aggttgggtg taaagtttag attcatctac atcagaatta tctacgccat cttcttcacc
     2341 accggttacg ccgatttcga tttcaagggt cataccgatt ttgtccatac gggctaaata
     2401 ttcacggcag atttccatat tttcttcgat tggctcttca gataaatcga tcatatgtga
     2461 agagaataac ggacgaccgg tttctgcgaa gtgtttttcg cctgcttcaa gtaagccatc
     2521 gatccaaggt aataagtttt tcgcagcgtg gtcggtgtga agaatcacag gcacaccata
     2581 ttcttctgct aattgatgaa cgtgttttgc acccgcaatc gcccctaata catctgcacg
     2641 agcgcctgaa gccggcttaa tgcctttacc cgcatagaac tgcgcaccac catttgagaa
     2701 ttgcacgatt accggtgatt ttacacgagc ggctgtttct aatacggcat taactgaatc
     2761 tgaacccacg cagtttactg ccggaatagc gaagttgtgt tctttagcat aagcgaaaac
     2821 tttttgtacg tcgtcgcctg ttactacacc cggttttacg atgtttaata atgacatttt
     2881 tttcttcctt tgtatgaaat gtgcaaaagc acattgttaa aatatacggc caaatcttgc
     2941 aaaattttca caaaatttga ccgcttgtaa ctgttagccg ttagctcgtt tttctaagat
     3001 ttccactgcc ggtaatactt taccttccac gaattctaag aacgcaccgc caccggttga
     3061 aatgtaagaa attttgtctt tgataccgaa taaatcaatc gcagctaagg tatcgccacc
     3121 accggcaatt gagaatgcac cgtttgccgt tgcttctgca atcgcatttg aaatcacttc
     3181 tgttccttta cggaagttag ggaactcaaa cacgccgacc ggaccattcc aaaggatggt
     3241 ttttgaatta cggataattt cagctaattg ctctgacgat ttttcaccaa tatcgaaaat
     3301 tgattcatca gctcgtactt cactcaccgc tttttccgtt gccggtgcag tttcagagaa
     3361 ttcagtacct acacgcacat caaccggaac aggaatattg gttacttgtg ctaaacgttt
     3421 tgcttcaggg attaaatctt cttcgtataa tgatttaccc acagcgtgac cttctgcggc
     3481 aataaaggta ttggcaatac caccgcccac gattaattgg tcggcaattt ttgaaagcga
     3541 atcaagcaca gttaatttag tagatacttt agaaccaccc acaatcgcta acattggacg
     3601 ttgtggctct tttaatgctt tgcctaatgc atctaattca gcggctaata acggacctgc
     3661 acacgcaaca ggggcatatt ctgccacacc gtaagttgaa ccttccgcac ggtgagctgt
     3721 accgaatgcg tccattacaa acacatcaca aagtgatgca tatttttttg ctaactcagg
     3781 gtcgtttttc ttctcacctt tgttaatacg cacgttttca agcaccacga tttcattctc
     3841 gtttacttca acaccgtcta agtaatcacg cactaaacga acaggaacac ctaaatctga
     3901 tgcatttaag taatcaacta caggttgtaa agagttttcc tcttcaaata cgccttctgt
     3961 cggacgacct aaatgcgaag ttaccattac tttagcacct ttttgtaacg ctaattttaa
     4021 ggttggaatg gtagcaacaa tacgggcatc tgatgttact ttgccatctt ttaccggtac
     4081 gttaaggtca gcacgaataa ataaacgttt acccgctaaa tcaaggtcag tcattttaat
     4141 aacagacata gtttgttcct ctttagatta aaatttaaaa gtgataacgg tcggattata
     4201 tcagtttcta atcattttgg gctaacctag atcaaataaa tgattaattt tttatcattt
     4261 tagaatgcta ttatcctgac tgttcttatt cccccccaat atgcttggtt aattaagcaa
     4321 gaattgcaaa ttccccacaa aatttaaccg cttgtcacta aaaataaagc tgtttgaaaa
     4381 tccattcaaa cagcttatag aatttagagt gaattaaaga aaatcaccaa gataatcacc
     4441 ggaatcacaa agcgaacata gttaaaccaa atagtggtaa acattttacc cgcacctaat
     4501 tcctccttcg cctcttcttt tagaacaaag ccaacgaaaa tcgcgctacc taaggcggtt
     4561 aaaataaaca gaatattgcc actaatataa tcgaaggcat caaaaatgct cttgcccata
     4621 acctgcacat cttgcaatat attgtcactt aatgctgatg gaatattacc caacagaaaa
     4681 atagtagcca aagtaataaa aatagctttt tgacggctaa tctttgcctt ctcttgcaat
     4741 gcagtgataa taacttcata aatagttaac gaggttgtta atgccgccac cactaataga
     4801 ctaaaaaaga taaccgcaaa aactgttccc ccccacatat ttgagaacac aatcggcaaa
     4861 ctttggaata ctaaagtcgg acctgaattt ggagcaacgc caaagctaaa taatgacgga
     4921 aaaatcataa aaccggcaag taccgcaatt aaggtgttca tcaccccagt aattgttgct
     4981 gtttttacta aattctcgct cttatcaagg tagcttgaaa gggttatcaa cacaccaaaa
     5041 ccaaggctta atgcaaaaaa tacttgaccc agcacaaaca caaacaattt tggtgtgatt
     5101 tttgaaaaat cgggcatcag ataaaaggca atgccttcag cagcccccgg taaggtaata
     5161 tttcgaacca ccatcacaat caggaaaata aacagaatcg gcatcaaata tttcactgaa
     5221 cgctcaatac cgtccacaat gccttttacc aaaataaaat aattcaccaa tacaaaaagt
     5281 gccgtataag caataattgt cattgggcta ttaaaaatac tttgttggaa gaattgttgg
     5341 gtcgtgccaa cggtaacagg ttgagacaga tctagtgtcc ccgtgagtaa gctaccgata
     5401 taatttaata cccaaccacc aagtaccata tagtaggcaa gaataccaaa tgctccaagc
     5461 aagcccatat agccaagaat tttccaacct gaggcaattt ttttaccatt agcaaaaccg
     5521 gcaaaagcat caatagcatt cactctcatt cggcgaccaa ttacattctc cactaatatc
     5581 atagggatac caattacgat catcgcaata caaaataaaa acacataagc accgcctccg
     5641 tgttccccca caagataagg gaaacgccaa gttgcaccaa aaccgactgt tgcaccagca
     5701 acggtcataa tatacgccaa tcggttagac caagattggc gttcttgttg ttgatttgcc
     5761 atttaatact ccatcggcta acaataccgc ttcaaccaac aagcggtcag atttttagaa
     5821 aatattacaa attaaatgaa gagtgcgaat aactcgccaa ctttaaccct tgagcccacc
     5881 ttctgcccta ttgaacgctg tacctgaatt tgggtaatat cggcagattg ataaatccta
     5941 cgggtaaaat cagtatcgtg atttgaaatt aatacgggaa cgcctaatcc tttcatttct
     6001 aaagccagtg ttgccaaacg cccttgttgc tctaggctaa aaccaccgcc agcatattgc
     6061 gtaaaattcg tttcttgtgc taaaggagcg taaggtgggt cgcagtaaat cacataatcc
     6121 gttaattgat tttttgccaa ctcaaagacc tgttcaaaat ccgcacacac aaaagttgcc
     6181 ttttgtgctt tttcagcaaa aaaacgtagt tctttttctg gaaaataatg ggttttataa
     6241 cgcccaaagg gaacattgta cccttttttc tggttataac ggcaaagtcc gttatagcca
     6301 aatcgattta aatacaaaaa aatcaccgag cgacgaaaaa tatcttttga gcgattaaac
     6361 tccgcacggc gtgctttata aaaggcttct gtattcgctt gtgggtgaag aaacagttgc
     6421 tttgtttgcg caatatattc ctcaacatct gctttcacca cgttgaaaag attaatcaag
     6481 tcaggattaa tatcagcaag aatataacgt tcaaaatctg tattcagaaa gaccgaacct
     6541 gcaccaacaa aaggctcgac taaacaagcc ttttttggca aatgggtttg aatatctgga
     6601 attaagcgat atttaccgcc agcccatttt aaaaaggctc gatgcttagg gtgattcatt
     6661 tcattttaaa cattaacgtt gggtaaaacg ctcaatagag gctaatactt gagattctgt
     6721 ggcttgcgat gaaacgtaag cttcaccaag tgatttcaat aaaattaggc gtaattgacc
     6781 tgccagtact tttttatctc gccacatata aggtagatat tctgccggtt ccattccatc
     6841 aggtgaaatt gttggcaaat tggctcgggc caataatttt tccaagcgag caacctcttg
     6901 aggacttaaa tcccccagaa tacgagataa ttccgcagcc tctagcatac caaccgaaac
     6961 agcctctccg tgtaaccata caccatagcc catacgtgct tcaatggcgt gaccaaaggt
     7021 atgtcctaag tttagtaatg cacgatctcc tttttccgtt tcatctcgag caaccacgtc
     7081 agcttttagc tggcaacaac gctgaataca atattccagc tcatcttgct tgagactgac
     7141 caaatcatca atatgctttt caagccattc aaaaaaagta acatcaaaga ttgcaccgta
     7201 tttgatgact tccgccaaac cagcactgac ttctcttttg gataaggtat ggagcgtatt
     7261 ggtatcaaca atcacagaaa caggctgata aaaagcccca atcatatttt taccgagtgg
     7321 gtggtttact gccgttttcc cgcctactga agaatcaact tgagccagta aagtggtcgg
     7381 aatttgtata aaacgaatac cacgttggta agaggcagct gcatagcctg caacatcacc
     7441 gattacacca ccgcctaaag caattaaagt ggtatcgcga ttatgatttt tttctaataa
     7501 tgcggtaaaa atcaaattta atgattctag cgttttatat tcttcaccgt cgggaatcaa
     7561 taccgaatca acgtggcaac ccagctcctt taacgcattt gttaccacac taagataatg
     7621 tgtcgccacc gttggatttg agacaatcat caccttatct ccagatttta atgggctata
     7681 actagcaggc tgatttaata accctgcacc aattataatc gggtaacgac gctcttttaa
     7741 ttccacattg acttgtaaca tatcttagct cctttcttac ctggctactg taaattatca
     7801 atcatatcaa taatttgatt tgccactact ttagcgctct gctcgtccgt atgaatagta
     7861 atatctgcaa tttcttcata caatgggtta cggattttcg ctaactcttc caacgtttta
     7921 cgggggtctt ctgtttgcaa taaagggcgt tttttatctc gctgagtacg ttcaaactgt
     7981 ttatccactg tcgtttctaa ataaataacg ataccacgag ccgataacac attacggtta
     8041 tctttagata aaatcgaacc accgccagtt gaaagcacga taccataatt ttgcgtcagt
     8101 tcatttagaa tacgttcttc acgcttacgg aaaccctctt caccttcaac atcgaagatc
     8161 caatcaatat ccgcacctgc tcgttcttcg atcacactgt cggaatccat aaattccata
     8221 cctaatgttt gagcaagctg acgaccaatc gtacttttac ctgctcccat cggcccaatc
     8281 aaaaaaatat tacgtttttc agccattatt tcttcttcat tttaaaattt actaaacatt
     8341 tgat
//
LOCUS       DANLRK010000112         8338 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.112, whole genome shotgun sequence.
ACCESSION   DANLRK010000112 DANLRK010000000
VERSION     DANLRK010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8338
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.112"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(<1..547)
                     /locus_tag="PXA33_001822"
     CDS             complement(<1..547)
                     /locus_tag="PXA33_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein Hlp"
                     /protein_id="HDL5121131.1"
                     /translation="MNTLAKISLSVLFTLFLTACEQPNSTKTQSSAESPVQVKEESKE
                     EVKPADTGAQDYKMLREWQDTQEKALNDAIKAATETLTDKQKADSTLMQETVNNALLA
                     QIDHIKISAETLNIQNNEVKALKDKTLEVLTLGAQMIVEGAKMEKNPTPEAHKAFGEL
                     QTKLNQLAEEGQQLENILRAKY"
     gene            complement(614..1198)
                     /locus_tag="PXA33_001823"
     CDS             complement(614..1198)
                     /locus_tag="PXA33_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein HlpB"
                     /protein_id="HDL5121132.1"
                     /translation="MNKLTKISATALVALFLAACDKPAEKAPATEPTKTTEAAPTTTA
                     PAAEAAKPAETAVAAPSEAAKADYQKLVDWNTAQESTMATAQAELQKKLATQDPKQIQ
                     EGLSAFNKKVEETVKSLDAIEVSDAQIKTFKEKTKSVLTLSSEVISEQVKTISTPNDQ
                     AALQAVQQKAQSLIEAGNELQKLNVELQQRFSAK"
     gene            complement(1330..2514)
                     /locus_tag="PXA33_001824"
     CDS             complement(1330..2514)
                     /locus_tag="PXA33_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HDL5121133.1"
                     /translation="MKSADIVIVGGGMVGLALAGLLKDVDCQIKIIEKNTPKFDADTI
                     YHRVSAINASSQKILEQIGAFQHIPAQRLSPYTEMKVWEKDSFANIHFDNNDNEIKQL
                     GLSQLGFILENAVIQHALWQQVSQQANVEYIIAAPQTLGVSENGAFLTLDNGEMLSAK
                     LVVGADGANSWVRNQMKMPLTSKDYQHTALVCNVKTTEKHGKTARQIFAEDSIFAFLP
                     MADEHLCSIVWSLPPNKANELKNASEAEFNKALTIAFDNQLGLCEVQSLREIYPLAAR
                     FCRDFAQPRIALIGDAAHTIHPLAGLGVNLGFADARTLANEIQKQLVNNQDIGEYRHL
                     RQFERERKAQAMKLLATMEGLKQLFDGNHPLKKLFRGIGLSLTNQLPFVKKQLIQQAI
                     YL"
     gene            complement(2597..3460)
                     /gene="rapZ"
                     /locus_tag="PXA33_001825"
     CDS             complement(2597..3460)
                     /gene="rapZ"
                     /locus_tag="PXA33_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HDL5121134.1"
                     /translation="MELIIISGRSGSGKSVALRALEDAGYYCVDNIPLPLIPTLTYYL
                     TQENRSAVISLDIRNLPNTPNAIEELLTQLEPLNAKLIFLDCERNTLIRRYSDSRRIH
                     PLSTQDDLSLEGALDLEQKLLEPLVQNANYIIDTSHLSSHELAENLRQILQGSTEKEL
                     KIIFQSFGFKYGLPADSDYVFDVRFLPNPHWNPELRPMTGLEQPVIDFLERQTEVHNF
                     IYQTRNYLEMWLPMLEQNNRSYLTIAIGCTGGKHRSIFIAEQLAKYFQAKGKNVQVRH
                     KSLEKHHKKTS"
     gene            complement(3473..3985)
                     /locus_tag="PXA33_001826"
     CDS             complement(3473..3985)
                     /locus_tag="PXA33_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL5121135.1"
                     /translation="MTKLTEFLSPENVRQGVLISSKKRALELVGKIVAESINQTEQQS
                     EALCPIECFGNLFKREKLGTTGLNQGIALPHAKLPACGDIELTKPIAVFLQLEEAIDY
                     EAQDNKEVDLIYAMMFPENSCEQYKSCLPAIAQKLTDKSLLKQLRAAESAEDIWQILV
                     YADNHLKDEE"
     gene            complement(4027..4752)
                     /gene="lptB"
                     /locus_tag="PXA33_001827"
     CDS             complement(4027..4752)
                     /gene="lptB"
                     /locus_tag="PXA33_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005727816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HDL5121136.1"
                     /translation="MSVLYAEHLAKSYKQRQVVKDVSLNVKSGEIVGLLGPNGAGKTT
                     TFYMVVGLVRHDHGTIRIDDQDISTLPMHDRAKQGIGYLPQEASIFRRLSVYDNLMSV
                     LQVRKNINDKQRKERAEELITEFHIEHIRNSLGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKKIIVNLKERGLGVLITDHNVRETLDVCERAYIVGSGEMIAS
                     GTPQQVLENPDVKRVYLGDQFKL"
     gene            complement(4764..5270)
                     /gene="lptA"
                     /locus_tag="PXA33_001828"
     CDS             complement(4764..5270)
                     /gene="lptA"
                     /locus_tag="PXA33_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HDL5121137.1"
                     /translation="MKLMFKSILVSVLLGSSFSAYALKGDTDQPINIDSGSQSLDMTN
                     NIVTFSDNVVITQGSIKVTAANVKITRQEGKKETIDATGSPVTFQQTLDNGKPVNGKG
                     NSVHYDLNSEFLTLIGNAELKQQGSFIKANKITYDVKKQQLKATSGGKTRVKTVLIPN
                     ELQDNKKK"
     gene            complement(5294..5878)
                     /gene="lptC"
                     /locus_tag="PXA33_001829"
     CDS             complement(5294..5878)
                     /gene="lptC"
                     /locus_tag="PXA33_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HDL5121138.1"
                     /translation="MNKRLTVILLIIVAVLGGWYYSLQEKENTGLDQLIKKEGQPEYV
                     GNKMSTTVYDLKGNPQYFAQATEIKRYESTERTEFFKPFIELFAKESTQKQWKVSADY
                     AEITKEKMLNLKGNIKLVALDPASRLQQITTDTLTVDLNTQDIFTESTVKSIGSGFTT
                     TGVGLKGNLKQQVATLQKDVKSYIEPTIIQETKE"
     gene            complement(6032..6835)
                     /gene="xthA"
                     /locus_tag="PXA33_001830"
     CDS             complement(6032..6835)
                     /gene="xthA"
                     /locus_tag="PXA33_001830"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HDL5121139.1"
                     /translation="MKFISFNINGLRARPHQLEAIIEKHQPDVLGLQEIKVADEDFPY
                     DLVNHLGYHVFHHGQKGHYGVALLTKKEPIAVRKGFPTDVLDAQKRMIMADIETDFGV
                     LTVLNGYFPQGENRSHETKFPAKQKFYADLQNYLESELKPENPIIIMGDMNISPTDLD
                     IGIGEPNRKRWLRDGKCSFLPEEREWLDRLYSYGLVDTFRAGNPDVNDKFSWFDYRSK
                     GFDDNRGLRIDLILASKSLAERCVETGIDLDIRAMEKPSDHSPVWATFK"
     gene            complement(6933..8309)
                     /locus_tag="PXA33_001831"
     CDS             complement(6933..8309)
                     /locus_tag="PXA33_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter family protein"
                     /protein_id="HDL5121140.1"
                     /translation="MLSNEVFISIIVLLILSLLRINVVIALIISALTCGLIGNLGVEG
                     AGFTEALEKTIKSFTGGLGGGAETAMNYAVLGAFAVALSKSGVTDLLAYKVIKSFGHS
                     PSGRSVTWFKYIVLFVLVAFAMSSQNLIPIHIAFIPILIPPLLSVMNKLQIDRRAVAC
                     ALTFGLTATYMLIPAGFGQIFIESILVKNINQAGEPFQLVTTTAEMTKAMLIPVSGMI
                     LGLLFAFFVSYRKPRVYIENMPEPTADDIEARVANVKPFHIGISIVAIIATCSVQLAT
                     NSTIIGGLVGLVIFALGGVFRLKESNDVFQAGLRLMAMIGFVMIAASGFAGVVNSTGG
                     VPALVESLRDVISSKEMAALLMLVVGLFITMGIGSSFSTVPIITSIYVPLCISFGFSP
                     LATMAIVGVAAALGDAGSPASDSTLGPTSGLNADGKHDHIWDSVVPTFIHFNIPLLVF
                     GWIAAMTL"
BASE COUNT         2318 a         1664 c         1680 g         2676 t
ORIGIN      
        1 catatttagc tcttaatata ttttctaact gttgcccttc ttcagctaat tgattcaatt
       61 ttgtttgtaa ctcgccaaat gctttatgtg cttccggtgt aggatttttc tccattttag
      121 caccttctac gatcatctga gcccctaaag tcaggacttc taaggttttg tcttttagtg
      181 ctttgacttc attattttga atattcaggg tttcagcact tatcttaata tgatcaattt
      241 gagctaataa tgcgttatta actgtctctt gcattaaggt agaatcggct ttttgtttgt
      301 cagtaagtgt ttctgttgcc gctttaattg catcatttaa ggctttttct tgagtgtctt
      361 gccattcccg taacatttta taatcttgag cgccggtatc agcaggttta acttcttctt
      421 ttgactcttc tttcacttga actggcgatt ctgctgagct ctgtgttttg gtagaattag
      481 gttgttcgca agcggttaaa aaaagggtaa ataatacaga taggctaatt ttagcgagcg
      541 tgttcatagt aatccttagt aattgcaaaa aaaagctaga gcaaaatact ctagcttttc
      601 attgaatggt aaattattta gctgagaatc tttgctgtaa ttctacgttt aatttttgta
      661 actcattacc agcttcaatt aaagactgag ctttttgttg tacggcttgt aatgctgctt
      721 ggtcatttgg tgtagagatc gtttttactt gttcagaaat cacttcactt gaaagtgtta
      781 aaactgattt tgttttttct ttaaatgttt tgatttgtgc atcagatact tcaatcgcat
      841 ctaagctttt tacagtttct tctacttttt tattaaatgc acttaatcct tcttgaattt
      901 gtttaggatc ttgtgtagct aatttctttt gtagttctgc ttgtgctgtt gccattgtgc
      961 tttcttgtgc cgtattccaa tcaactaatt tttggtaatc tgcttttgca gcttcgcttg
     1021 gagcggctac agctgtttca gcaggtttgg ctgcttctgc agcaggagct gttgtagttg
     1081 gtgcagcttc agttgttttt gtaggttctg ttgccggtgc tttttctgct ggtttatcac
     1141 aagctgctaa gaataatgcg actaaagcgg ttgcactgat ttttgttaat ttgttcatta
     1201 aaaaattcct caagaatata aaaataaaag ccaataaagg ctggttattg taccaataaa
     1261 tagacaagta atctccgtat tagttcataa aatcgttcat ctttacgtta tttcgaaaaa
     1321 atttacattt tataagtaaa tagcttgctg aataagctgc tttttcacaa aaggcagctg
     1381 gttggttagg cttaaaccaa ttcctctgaa taatttttta agcgggtgat tgccgtcaaa
     1441 tagctgtttt aatccttcca ttgtggctaa taatttcatc gcttgggctt tacgctcacg
     1501 ttcaaattgg cgtaaatggc ggtattcacc aatatcttgg ttgtttacca actgtttttg
     1561 gatttcgttt gccagcgttc tggcatcggc aaagcctaaa ttgacaccaa gtcccgccaa
     1621 tgggtgaatg gtatgagcgg catcaccaat caacgcaata cgaggttggg caaagtcacg
     1681 gcagaagcgt gctgcaagcg ggtaaatttc acgcaaactt tgcacttcac ataagccaag
     1741 ctgattatcg aaggcaatcg ttaaggcttt attaaattcg gcttcgcttg cattttttaa
     1801 ttcattggct ttgtttggtg gcagcgacca aacaatcgag cagagatgct catctgccat
     1861 tggcagaaaa gcaaaaatgc tatcttcagc aaagatttga cgagcggttt tgccgtgctt
     1921 ttcggttgtt ttgacattac agactaaggc ggtatgttga taatctttac tggtaagcgg
     1981 cattttcatc tggttacgca cccacgaatt tgcaccgtct gccccgacaa ccaatttagc
     2041 agacagcatt tcgccgttat ctaaggttaa aaatgccccg ttttcgctca cgcccagagt
     2101 ttggggggcg gcgataatat attctacatt agcttgttga gaaacctgtt gccacaatgc
     2161 gtgttgaatc accgcatttt ccaaaataaa gccaagttgt gaaagtccta attgcttgat
     2221 ttcattatca ttattatcaa aatggatatt agcaaagctg tctttttccc acactttcat
     2281 ttcggtgtaa ggagaaaggc gttgagccgg aatgtgttga aaagcaccaa tctgttccag
     2341 tattttttgg cttgaagcat taatcgcact tacacggtgg taaatcgtat cggcatcaaa
     2401 tttgggcgtg tttttctcaa taattttaat ttggcaatcg acatctttga gcagtccggc
     2461 aagagccagc ccaaccatac cgccgccaac aataacaata tctgcggatt tcataaaatc
     2521 aaatgtaggg gcggtgccct gtgcccgccc taaaatagtt gaaatgaggg ctggcacagg
     2581 accagcccct acttatttaa gatgtttttt tatggtgttt ttccaaactt ttatgccgaa
     2641 cttgcacatt tttgcctttt gcttggaaat attttgccag ttgctcggca ataaatatgg
     2701 aacgatgttt tccaccagtg caaccaatcg caatagttag gtagctacgg ttattttgtt
     2761 ccaacatcgg cagccacatt tctaaataat tgcgggtttg gtaaataaaa ttatgcactt
     2821 cggtttggcg ttctaagaaa tcaatcaccg gttgctctaa gcctgtcatt gggcgaagct
     2881 ccggattcca atgtgggttc ggtaaaaagc gaacatcaaa aacgtaatcg gaatcggcag
     2941 gtaagccgta tttaaaaccg aatgattgga aaataatttt aagctctttt tcggttgagc
     3001 cttgcagtat ttggcgaaga ttttccgcta attcgtgcga agaaagatga ctggtgtcaa
     3061 taatataatt tgcattttgc accaaaggct caagcaattt ttgttccaaa tccaatgcac
     3121 cttctaacga gagatcgtct tgggtggaaa gcgggtggat tcggcgggaa tcgctataac
     3181 ggcggattaa ggtattgcgt tcgcaatcta aaaaaatcag cttagcattt aaaggctcaa
     3241 gctgggtaag caattcttca atcgcattag gcgtattcgg taaattacga atatctaagc
     3301 tgatgactgc cgaacgattt tcttgagtaa ggtagtaggt taaggttgga attaaaggta
     3361 aagggatatt atccacgcag taataacctg catcttctaa tgctcttaat gctaccgatt
     3421 taccagagcc tgagcgtccg cttataatga ttaattccat atttcctcag tattattctt
     3481 catcttttag gtgattatca gcatacacca aaatttgcca aatatcttct gcggattcag
     3541 ctgctcggag ctgttttaat aacgatttat cggtaagttt ctgtgcaatt gcaggtaggc
     3601 aacttttata ctgttcgcag ctattttccg gaaacatcat tgcataaatt aaatccactt
     3661 ctttgttgtc ttgggcttcg tagtcaatcg cttcttccag ttgtaaaaag accgcaatcg
     3721 gtttagtcag ctcaatatca ccgcaagcgg gcaattttgc atgaggaagt gcaatgcctt
     3781 gatttaagcc ggttgtgcct aacttttcac gtttaaataa attgccaaag cattcaatag
     3841 ggcagagtgc ttcagattgc tgttcggttt ggttgattga ttcagccacg attttaccca
     3901 cgagttctaa cgctcgtttt ttacttgaaa tcagtacacc ttggcgaaca ttttcagggc
     3961 ttagaaattc ggttagtttt gtcataattt ttcatctaaa gatgaaatcc ccaaagaggg
     4021 gattttttag agtttgaatt gatcgcctaa ataaacacgt tttacatcag ggttttcaag
     4081 cacttgctgt ggtgtgccgc tggcaatcat ttcgccgcta ccgacaatgt aggctctttc
     4141 acatacatct agagtttcac gtacgttatg gtcggtaatc aataccccta aaccacgctc
     4201 ttttaaattg acgataattt ttttaatatc aattaccgag attgggtcaa ccccagcaaa
     4261 aggttcatct aacagaataa atttcgggtt cgctgctaat gctcgggcaa tttccacacg
     4321 gcggcgttca ccacctgaaa gtgattgacc taaactattg cgaatatgct caatatggaa
     4381 ttctgtaatc agctcttcgg cacgctcttt acgctgttta tcattgatat ttttacgcac
     4441 ttgtaatacc gacattaagt tatcgtacac acttaaacgg cggaaaatag atgcttcttg
     4501 cggtagatag ccaatccctt gttttgctct gtcgtgcatt ggtagtgtgc tgatatcttg
     4561 gtcgtcaata cgaattgtgc cgtggtcgtg gcgaaccaaa cccacaacca tatagaaagt
     4621 ggtggtttta cctgcgccgt tcggaccgag caagcccacg atttcacctg atttgacatt
     4681 caggctgacg tcttttacca cttggcgttg tttatagctt tttgctaaat gttcggcata
     4741 aagcacagac atatcattta accttatttt ttcttgttat cttgaagttc atttggaatt
     4801 agcacggttt tcactcgggt tttaccgcca ctggtagctt taagttgttg ttttttcaca
     4861 tcataggtaa ttttattggc tttaataaag ctaccttgtt gtttgagttc cgcattgcca
     4921 atcagcgtta aaaattcaga gtttaaatca taatgaacgc tgttgccttt accgtttacc
     4981 ggtttaccgt tatctaaggt ttgttggaat gttacaggtg agcctgtagc gtcaatagtt
     5041 tcttttttac cttcttgacg ggtaattttg acgtttgctg cggtcacttt aatcgaccct
     5101 tgagtgataa cgacattatc gctaaaggtc acgatattgt tagtcatatc taacgattgg
     5161 ctacctgaat caatattaat cggctgatcg gtatcgcctt ttagagcata agcggaaaag
     5221 ctgctaccca gtaacacgct cactaaaata gatttaaaca ttaatttcat tgttattcct
     5281 tgttccaagc aaattattct ttagtttctt gaataatggt cggttcaatg taagatttta
     5341 catctttttg caaagtcgca acttgttgtt ttaagttacc ttttaatcca acccccgttg
     5401 tagtaaaacc ggaacctatc gatttgaccg tactttcggt aaaaatatcc tgtgtattta
     5461 aatcaacagt taacgtatcg gtagtgattt gttgcaaacg tgatgcagga tctaatgcaa
     5521 caagtttgat attacctttt aaatttaaca ttttttcttt ggtaatttct gcataatcgg
     5581 cactgacttt ccactgtttt tgcgtcgact ctttggcaaa aagctcaata aaaggtttaa
     5641 aaaactcagt acgctcagta gactcatagc gtttaatttc agtagcttga gcgaaatatt
     5701 gtgggtttcc ttttagatca tagaccgtgg tggacatttt attccccaca tattcaggct
     5761 gtccttcctt tttaataagc tgatcaagcc ccgtattctc tttctcttgt aagctgtaat
     5821 accagccacc aagtacggca acaataatca gtaaaataac ggttaaacgt ttgttcataa
     5881 tccaaaaata atctaaaaat agtgtttgat tgtagcacag atttgaatag ggatatagta
     5941 cagcgtaaaa agtgcaaaaa agtgagaaaa aataaccgct tgtagggcta aaaactgaca
     6001 agcggttgaa aactcagatt tttttgcaac tttatttaaa ggttgcccaa acaggggagt
     6061 ggtcagacgg tttttccatt gctcgaatgt ctaaatcaat gcctgtttcc acgcaacgct
     6121 cagcaagtga ttttgaagca agaattaaat caatacgcaa gccacgatta tcatcaaaac
     6181 cttttgaacg gtagtcaaac caagagaact tatcgttcac gtctgggttg cctgcacgga
     6241 aagtatcaac caagccgtag ctgtataagc gatctagcca ttcacgctct tcgggtaaga
     6301 atgaacattt accgtcacgc agccagcgtt tgcggtttgg ctcgccgatg ccgatatcca
     6361 aatcagtcgg gctgatgttc atatcgccca taataataat cggattttcc ggctttaatt
     6421 cgctttccaa gtagttctgc aaatcagcat agaatttttg ttttgccggg aatttggttt
     6481 cgtggctgcg attttccccc tgtgggaaat agccgtttaa tacggttagc acgccgaaat
     6541 cggtctcgat atccgccata atcattcgtt tttgagcatc aagtacatct gtcgggaagc
     6601 ctttacgcac cgctataggc tcttttttag tcagtaatgc cacgccgtaa tgacctttct
     6661 gcccgtggtg gaaaacgtgg tagcctaagt gattgactaa atcgtaagga aaatcttcat
     6721 cggcaacttt gatttcttgt aagccgagta catcgggctg gtgtttttcg ataatcgctt
     6781 cgagctgatg tggtctggct cgtaatccgt tgatattaaa agagataaat ttcattggtg
     6841 atcctaatgt ggtttgcaaa ataaagaaga aaatagaccg cttgtaatgg ctacaagcgg
     6901 tcatttttag caaaaatttt gcatattata gcttaaagtg tcattgctgc aatccagccg
     6961 aacactaata atggaatgtt aaagtggata aaagttggta caacagaatc ccaaatgtgg
     7021 tcgtgtttac cgtcagcatt caagccggag gttggaccaa gtgtggaatc tgatgccgga
     7081 gaacctgcat caccgagtgc agcagcaaca ccgacaatcg ccattgtcgc cagtggcgag
     7141 aagccaaatg agatacaaag cggcacataa attgaggtaa tgatcggcac agtcgagaat
     7201 gaagagccga tacccatcgt aataaataag ccaacgacta acattagcaa tgctgccatt
     7261 tcttttgatg aaatcacatc acgcaggctt tctaccaatg ccggcacacc gcctgttgaa
     7321 ttgaccacgc cggcaaaacc ggaggcggca atcatcacaa agccaatcat cgccattaaa
     7381 cgtaagccag cttggaagac atcgttactc tctttcagtc taaatacacc gcctaaggcg
     7441 aaaataacca gaccgactaa gccgccgata atagtcgaat ttgttgctaa ttgcaccgaa
     7501 caggtcgcga taatcgccac aatactaatg ccgatatgga agggcttaac gtttgcaaca
     7561 cgggcttcaa tatcatctgc ggttggttca ggcatatttt caatatacac acgcggttta
     7621 cggtatgaca caaagaatgc aaacagtaag cctaaaatca taccggaaac aggaattaac
     7681 atcgctttgg tcatttccgc tgtggtagtc actaattgga acggttcacc tgcttggttg
     7741 atatttttaa ctaagatgct ttcaataaag atctgaccga aacctgccgg aattaacata
     7801 taagtggcgg ttaaaccaaa ggttaaagca caagctaccg cacggcggtc aatttgcaat
     7861 ttattcatta cgcttaatag cggtggaatt aaaatcggaa taaaggcaat atgaattgga
     7921 attaggtttt gagatgacat tgcaaatgcc actaacacaa ataatacaat gtatttaaac
     7981 caagtaacgg agcgacctga cgggctgtga ccaaaggatt tgatcacttt ataggctaat
     8041 aaatcggtca cgcctgattt tgatagagct acggcaaacg caccaagaac ggcatagttc
     8101 atagcagttt ccgcaccccc acctaaacca ccagtaaaac tttttattgt tttttccaat
     8161 gcttcggtaa atccagcccc ttctacacca agattaccga ttagaccgca agtaagggca
     8221 gaaataataa gtgcgattac tacattaatt ctaagcaagc ttaggattaa cagcacgata
     8281 atcgaaataa agacttcgtt tgataacatt gtgtttgatc cttaggttat ttaaataa
//
LOCUS       DANLRK010000113         8278 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.113, whole genome shotgun sequence.
ACCESSION   DANLRK010000113 DANLRK010000000
VERSION     DANLRK010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8278
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.113"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(374..2044)
                     /gene="glnS"
                     /locus_tag="PXA33_001832"
     CDS             complement(374..2044)
                     /gene="glnS"
                     /locus_tag="PXA33_001832"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HDL5121141.1"
                     /translation="MSEEILTAETGTRANFITHIIDEDLASGKHNNVYTRFPPEPNGY
                     LHIGHAKSICLNFGIAQDYNGLCNLRFDDTNPVKEDVEYVDSIKQDVEWLGFKWEGEP
                     RYASDYFDQLYGYAIELINKGLAYVDELSPDEMREYRGTLTEPGKNSPYRDRSVEENL
                     ALFEKMKNGEFAEGKACLRAKIDMASPFIVMRDPVLYRVKFAHHHQTGDKWCIYPMYD
                     FTHCISDAIERITHSLCTLEFQDNRRLYDWVLDNISIERPLPHQYEFSRLNLEGTLTS
                     KRKLLKLVTDGIVDGWNDPRMPTISGLRRRGYTPASLREFCRRIGVTKQDNVVEYSAL
                     EACIREDLNENAPRAMAVINPVRVVIENFGEKEILKAPNHPNRPELGERDLPFTREIY
                     IDEADFREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDASGRISTIFCTYDPDTLGK
                     NPADGRKVKGVIHWVSAEDNKPAEFRIYDRLFTVPNPGAEEDINAVLNPESLVVKHGF
                     VESSLAHAKPEQDYQFEREGYYCLDSKDGSADNLVFNLTVSLKESVAF"
     gene            complement(2205..2648)
                     /locus_tag="PXA33_001833"
     CDS             complement(2205..2648)
                     /locus_tag="PXA33_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HDL5121142.1"
                     /translation="MNLQQIQDRLALKDLVDTFSNLADEKKVAEQMPLFTQDAVVNTY
                     IGGKLVFEMHGVAEIEQVFTTFLTPFHSVYHLNGQHTVPFIDENNATAINYCAVKLVE
                     TKEGKEILHDHSVRYADTYVKQEGKWRIAKRIANFMISESREIRQ"
     gene            complement(2699..3274)
                     /locus_tag="PXA33_001834"
     CDS             complement(2699..3274)
                     /locus_tag="PXA33_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HDL5121143.1"
                     /translation="MKQILILGASGSLANVVIPELLKDNNVQLTLFARRPQSVAKFET
                     ERVKIVQGDVLDFHALTQAMQGVDAVYAGLAGDLKAMAGNVVNAMNEAQVKRLVWISS
                     MGIYGETGEDHGAILEPYRQSAAVIEASELDYTVVRPGWFTNGSEVQYQLTHKGEPFK
                     GHSVSRKSIADFVARILRENSEIRESVGIAA"
     gene            3381..4271
                     /locus_tag="PXA33_001835"
     CDS             3381..4271
                     /locus_tag="PXA33_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL5121144.1"
                     /translation="MKENLNDLRAFLLVAQTSSFTKAAAQMGVSQSALSHSIRGIEER
                     LQIKLFHRTTRSISTTEAGERLYQRLLPLFDEIDQEVNGLSEFRNALKGGLRINGNEH
                     VFRDVLADKFAEFVKRYPEVALELVAEDRFVDIVAERFDAGIRLGADVAKDMIALRIS
                     PDVKMTAAASPNYLAQHGTPKTPFDLTEHHCLKHRLATLGGILAWEFRDPITKKIVKF
                     QPQGNLTANNGFLLQHYAKQGLGILWSPQDSIQTELDNGSLVPILTDWAMQYEGYHLY
                     YPNRRQNSPLLKALIDVLRG"
     gene            complement(4339..4905)
                     /gene="efp"
                     /locus_tag="PXA33_001836"
     CDS             complement(4339..4905)
                     /gene="efp"
                     /locus_tag="PXA33_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HDL5121145.1"
                     /translation="MASYSTNDFKKGLKFIQDGEPCVIVENEFVKPGKGQAFTRTKIR
                     KLISGKVLEINFKSGTSVEAADVVDSNYNYSYSDGDFWYFMHPETFEQISVDEKALGD
                     NVKWLVDNAECIVTLWNGSAIAVTPPNFVELEIVETDPGLKGDTAGTGGKPATLSTGA
                     VVNVPLFVQIGEVIRVDTRSGEYVSRVK"
     gene            5072..5641
                     /locus_tag="PXA33_001837"
     CDS             5072..5641
                     /locus_tag="PXA33_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121146.1"
                     /translation="MKKLITLSAILAVSVATVAEAKRGGGSFRSVSPSKAVATKPVAS
                     QKSTTNQQTQQKDATFANTPNSATAQPQVRQGSTMANVAMGAAAGYILGDMMSSNAHA
                     NQPSQPADANAQAVNSAQNVANGAVPAIAEFKSIGGQIDPFLVEKTDGYRRYCISGVQ
                     YLVAAQGVQTSPTVMVNPNGTPLQCNLIP"
     gene            5695..6687
                     /gene="epmB"
                     /locus_tag="PXA33_001838"
     CDS             5695..6687
                     /gene="epmB"
                     /locus_tag="PXA33_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HDL5121147.1"
                     /translation="MQPIQIKPLWLTELAQAFNNPIDLLQFLELNPNDFEGDIAARKL
                     FALRVPRMFAEKMEKGNPNDPLFLQAMSLQAEFIEAEGFVVDPLEEQQSPAPNILHKY
                     HNRLLFMLKNSCAINCRYCFRRHFPYEEVKSGKAVWQQGLTYIAEHPELEEVILSGGD
                     PLMAKDQDLDWILTQLEQISHIKTLRIHSRLPVVIPNRITTELCERLSKSRLNVVLVT
                     HINHANEIDAVFANKMAQLKKAGVVLLNQSVMLKGVNDNAQTLKRLSDKLFEYGILPY
                     YLHLFDKVAGASHFYIEDEQAGEIYRELQRITSGYLVPKLAREIAKEPNKILKG"
     gene            complement(6787..8190)
                     /gene="asnS"
                     /locus_tag="PXA33_001839"
     CDS             complement(6787..8190)
                     /gene="asnS"
                     /locus_tag="PXA33_001839"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HDL5121148.1"
                     /translation="MSKFPTVSEILSGKIAVGEEATVRGWVRTRRDSKAGLSFLSVYD
                     GSCFDPIQAIINNDLPNYNAEVLHLTAGCSVVVTGKVVESPAEGQAVELQASKVEVLG
                     WVEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHCLAQAIHRFFHEQGFYW
                     VATPVITASDTEGAGEMFRVSTLDLENLPRGENGKVDFNQDFFGKESFLTVSGQLNAE
                     TYACALSKVYTFGPTFRAENSNTTRHLAEFWMMEPEVAFADLNDNAKLAEDMLKYVFK
                     AVLAERKDDMEFFVKHVDKEAISRLENFINSPFAQVDYTDAIEILLKSGKEFEFPVSW
                     GIDLSSEHERFLAEEHFKSPVVVKNYPKDIKAFYMRLNDDGKTVAAMDVLAPGIGEII
                     GGSQREERLEVLDQRMNEMGLNPEDYWWYRDLRKYGTVPHSGFGLGFERLIVYVTGLQ
                     NIRDVIPFPRAPRNANF"
BASE COUNT         2372 a         1782 c         1719 g         2405 t
ORIGIN      
        1 tcttatctat tcttcgagaa gacaatgcta tcataaaaga gcatcacttt tggtttccaa
       61 agattataga aagctcaaat aatacactac ctcataattt aaaatgaaac gtttcaattg
      121 gaaaaagtgg attttgtgac tgagatcaca aatttaaaca ttggtctgta tttttgagag
      181 attttgtttg aaactgccgg caaattcgct taaccttaag gtaacaagaa taagtgttgt
      241 acctagcaac ctaacacata aatttgcttt ccgagtggcc ccaagaattg gggctttttt
      301 tatgcttgat gagtcgtttt gagagtaaaa aaataccagc atatttgctg gtatttgaat
      361 gaagagagtt agattagaac gctacgcttt cttttaagct gacagttaag ttaaatacaa
      421 ggttgtctgc cgaaccatct ttgctgtcta agcagtagta accttcacgc tcgaattggt
      481 agtcttgttc cggtttggcg tgagctaaac ttgactcaac aaaaccatgc ttaaccacta
      541 atgattcagg atttagcacc gcattgatgt cttcttccgc ccccgggttt ggtactgtaa
      601 ataggcggtc gtaaatgcgg aactctgccg gtttgttatc ttcagcagat acccaatgaa
      661 tcacgccttt tactttgcga ccatcagccg gatttttgcc caatgtatcc gggtcgtatg
      721 tgcaaaaaat agttgaaatt cgaccgcttg catctttttc cacacgctca gctttaatca
      781 cataggcatt gcgtaaacga acttctttgc ctaagactaa acgtttatat tgcttattgg
      841 cttcttcacg gaagtcggct tcatcaatat aaatctcacg ggtgaacggt aaatcacgct
      901 cgccaagctc tggacgattt gggtgattag gggcttttaa tatttccttt tcaccgaagt
      961 tttcaatcac cactcgaacc gggttaatca ccgccatcgc gcgtggtgcg ttttcattta
     1021 agtcttcacg aatgcacgct tcaagggcag agtattccac cacgttatct tgcttggtta
     1081 ccccaatacg gcgacaaaat tcacgtaaag acgctggggt ataaccacgg cggcgtaagc
     1141 cggaaatggt tggcatacga gggtcattcc aaccatcaac aatgccgtct gtgactaatt
     1201 ttaataattt acgcttagag gttaaagtac cttctaaatt taaacgtgaa aattcgtatt
     1261 ggtgcggtaa cggtctttca atcgaaatat tgtccagcac ccaatcatat aagcggcggt
     1321 tatcttggaa ctctagcgta catagcgagt gagtgattcg ctcaatcgca tcggaaatgc
     1381 agtgggtaaa atcgtacatc gggtagatgc accatttatc accggtttga tgatggtgag
     1441 caaatttcac acggtaaagc accggatctc gcatcacaat aaacggggac gccatatcaa
     1501 tttttgctct caagcaggct ttcccttccg caaactctcc gtttttcatt ttttcgaata
     1561 acgctaagtt ttcttccacg ctacgatcac ggtaagggct gtttttgccc ggctcggtta
     1621 aagtaccacg gtattcacgc atttcatcag gcgataattc atcaacatag gctaatcctt
     1681 tgttgattaa ctcaatcgca tagccataaa gctggtcaaa atagtctgag gcataacgag
     1741 gctcaccctc ccatttaaaa cctagccact ccacatcttg cttaattgaa tctacatatt
     1801 ccacgtcttc tttaaccggg ttggtatcat caaaacgcaa gttacataaa ccgttataat
     1861 cttgagcgat accaaaattt aaacagatag atttagcgtg tccgatatgc aaataaccgt
     1921 ttggttcagg tggaaaacga gtgtaaacat tgttgtgctt tccgcttgct aaatcttcat
     1981 caataatatg ggtaataaaa ttggctcgag tgccagtttc tgccgttaaa atttcttcgc
     2041 tcatagttct ctctaaatgt atcaataaaa ataacggatt attctacact taattgaaac
     2101 agacttaaag actaaagcaa aattcctcgg tttcctccaa agaattttgc tgttttagta
     2161 agatgcaatg gaattgcaaa tttttagcta aatttaaccg cttgttattg cctgatttca
     2221 cggctttcgc tgatcataaa attcgcaatc cgtttggcaa tccgccattt accctcttgt
     2281 ttaacatagg tatcggcata acgcacgctg tgatcgtgca agatctcttt gccctctttg
     2341 gtttctacca gtttcaccgc acaatagttg atcgctgtgg cgttattttc atcaataaaa
     2401 ggcacggtat gctgaccgtt taaatgataa acgctgtgaa acggggttaa aaaggtggta
     2461 aacacctgtt cgatttctgc cacgccgtgc atttcaaaca ccaattttcc gccgatgtag
     2521 gtgtttacga cggcatcttg cgtgaaaagc ggcatctgct cggcaacctt tttttcatcg
     2581 gcaaggttag agaatgtatc tactaaatct tttagtgcta aacgatcttg gatttgttgt
     2641 aagttcattg tgttttcctt ctgatttttg caaattttct ttggtttttc accgcttgtt
     2701 aggctgcaat accaacactt tcacggattt cgctgttttc acgcaaaatg cgagccacaa
     2761 aatcggcaat ggatttgcgg gaaacgctgt gtcctttgaa cggttcgcct ttgtgagtca
     2821 gctgatattg cacttccgaa ccgttggtaa accagcccgg gcggacaacg gtataatcca
     2881 actcgctggc ttcaatcacg gctgccgatt ggcggtaagg ctctaaaatt gccccgtgat
     2941 cttcgccggt ttcgccataa atccccattg agctaatcca aaccaagcgt tttacctgag
     3001 cttcattcat tgcgttcacc acatttcccg ccatcgcttt taagtcgccg gcaagtccag
     3061 cgtaaacggc atcaacgcct tgcattgctt gggttaatgc gtggaaatcc aacacatcgc
     3121 cttgcacaat tttgacgcgt tcagtttcaa atttcgctac tgattgcgga cgacgggcaa
     3181 aaagggttaa ttgcacatta ttgtctttta gcagttcagg aatcacgaca ttcgctaggc
     3241 taccgcttgc tcctaaaatt aagatctgtt tcattgctgt tctccattga atgaagtcag
     3301 tatagcggtg tagattgcat ttgattagta gggtaaaatc gcttgaagta atgagaaaaa
     3361 ttcattaata aggtggttaa atgaaagaaa acctcaacga tttaagagct tttttgttgg
     3421 tagcacaaac gagcagtttt accaaagcag cagcacagat gggcgtgtcg cagtcggcgt
     3481 tgtcgcacag cattcggggg attgaagaac ggttgcaaat caagctgttt catcgcacca
     3541 ctcgtagcat ttcaaccacc gaggcagggg agaggctgta tcaacggctg ttaccgctgt
     3601 ttgatgaaat cgaccaagaa gtaaatgggc tgagtgagtt tcgcaatgcg ttaaaaggcg
     3661 ggctgagaat taacggtaat gaacacgttt tccgtgatgt gctggcagat aaattcgctg
     3721 agtttgtcaa acgttaccct gaagtagcat tggagctagt ggcggaagat cgctttgtag
     3781 atattgtcgc cgagcgtttt gatgccggta ttcggcttgg ggcagatgtg gcgaaagata
     3841 tgattgcact acgcatttcg cccgatgtga aaatgaccgc agctgccagc cccaactatt
     3901 tggcacagca cggcacgccg aaaacgccct ttgatttaac cgaacatcat tgtctcaaac
     3961 atcgtcttgc gacattgggc ggcattctgg cgtgggaatt tcgggatccc atcaccaaga
     4021 aaatcgttaa atttcaaccg cagggcaatc ttactgccaa taacggcttc ttgctgcaac
     4081 actatgccaa acaaggttta ggcattcttt ggagtccgca agacagcatt caaaccgagc
     4141 tggataacgg cagcctcgtg ccgattttaa ccgactgggc aatgcagtat gagggctatc
     4201 atctctatta ccccaaccgt cgccaaaatt cgcctttgct gaaagcgtta attgatgtgc
     4261 tgagaggata ggtaaaaatg agcggtcgtt tttagcaaaa aaattgacca aaaaacgacc
     4321 acttgtagag aagctaaatt atttcacacg agaaacatat tcgcctgaac gggtatccac
     4381 acggataact tcaccgattt gaacgaataa aggcacgttt accacagcac cggtgcttaa
     4441 tgttgccggt ttaccgcctg tacctgcagt atcgcctttt aagcccgggt cggtttcgac
     4501 gatttctaac tcaacgaagt ttggtggtgt gactgcaatt gctgaaccgt tccataaagt
     4561 cacgatacat tctgcattat caactaacca ttttacgttg tcgcctaacg ctttttcatc
     4621 taccgagatt tgttcaaagg tttcagggtg cataaagtac cagaaatcac catcgctgta
     4681 agagtagttg tagttagaat ctactacatc tgctgcttca accgaagtgc ctgatttgaa
     4741 gttgatctct aatactttgc cagagattaa tttacggatt ttagtacggg taaatgcttg
     4801 acctttaccc ggttttacga attcgttttc aacaattaca caaggttcgc catcttgaat
     4861 aaattttaaa ccttttttaa agtcgttagt gctgtagcta gccattattt cctcaattat
     4921 ttaaaaaata acaataaatt aacaaatttt gaacccaacc ctattgggtt tgtcaaaaag
     4981 agccatataa taacctaaat tcacaaaatt agcgaaagat ctcttgcgat ttttgctata
     5041 atttttcaaa ctttattgat aaggaatgcg aatgaaaaaa ctgattactc tttctgcgat
     5101 tttagccgtt tctgttgcaa ccgtggcaga ggcaaaacgt ggcggtggga gcttccgttc
     5161 agttagcccg tcaaaagcgg tagcaactaa gccggtagct tctcaaaaat caaccacaaa
     5221 tcagcaaacg caacaaaaag atgctacttt tgccaatacg ccaaacagtg caactgccca
     5281 gccacaagtg cgtcaaggca gcacgatggc aaatgtagcg atgggggcag cagccggtta
     5341 tattttaggt gatatgatgt catcaaatgc acatgcaaat cagcctagcc agcctgcgga
     5401 tgcaaatgca caagcggtca attctgcaca aaatgttgca aatggagcag ttcctgctat
     5461 tgcagaattt aagagtattg gcggtcaaat cgatccgttt ttagtagaaa aaaccgatgg
     5521 ttatcgccgt tattgtattt caggcgtgca atacctagtc gctgcacaag gtgtacaaac
     5581 ctcaccaact gtaatggtta atccaaatgg cacgccatta caatgtaact taattccata
     5641 attattttta tttgcgggca ggcaaaaacc tgccccttgt atctacaata ttcaatgcaa
     5701 ccaattcaaa tcaaaccgct ttggctaacc gagctggctc aagcctttaa taaccctatc
     5761 gacttattac aatttcttga actcaatcca aatgattttg agggcgatat agctgcccgt
     5821 aaattatttg ctcttcgagt gccaagaatg ttcgccgaaa aaatggaaaa gggtaaccca
     5881 aacgatccgc tttttttgca agcaatgtct ttacaggcgg aatttattga ggcagaaggg
     5941 tttgtggtag atccattgga agagcaacaa agcccagcac cgaatatttt acataaatac
     6001 cataaccgtt tgttatttat gctaaaaaac agttgtgcga ttaactgccg ttactgtttt
     6061 cgccgtcatt tcccttatga ggaagtgaaa agcggtaaag ctgtgtggca acagggacta
     6121 acttatattg ctgagcatcc cgagctggag gaagtgattc tgtccggtgg tgatccgctg
     6181 atggcaaaag atcaggactt agattggata ttaacgcaat tagagcagat ttcgcatatc
     6241 aaaaccctgc gtattcattc acgcttgccg gtcgttattc ccaatcgaat tacgacagaa
     6301 ttatgtgaac ggttatcaaa atctcgcttg aatgtcgtgt tggttaccca tattaatcac
     6361 gccaatgaaa ttgatgctgt ttttgcaaac aaaatggctc aactgaaaaa agccggcgtt
     6421 gtattgctga atcaatcggt gatgttaaaa ggtgtaaacg ataatgctca aaccttaaaa
     6481 aggttaagtg ataaactttt tgaatatggg attttgcctt actatttgca tttatttgat
     6541 aaagtggcag gggcgagcca tttctatatt gaagatgaac aggctggtga gatttaccga
     6601 gaattacagc gtatcacttc aggctatctt gtgccaaagc tggcaagaga aatagcgaaa
     6661 gagccaaata aaattttgaa aggttagctt aaacagaaat caagaaacaa aaggcgaaaa
     6721 tggtaaaatt ttcgcctttt ttaaccgctt gttgcaagcg gttaaattct cttacttttt
     6781 tgcaaattag aagttcgcat tgcgtggagc acgtgggaat gggattacat cacgaatgtt
     6841 ttgtaacccg gttacataca cgattaaacg ctcgaaacct aaaccgaaac ctgagtgtgg
     6901 cactgtaccg tatttacgca aatcacgata ccaccagtaa tcttcagggt ttaagcccat
     6961 ttcattcata cgctgatcta acacttctaa acgttcttca cgttgagaac caccgataat
     7021 ttcaccgatt cccggagcta aaacgtccat tgcggcaacc gttttgccgt catcatttaa
     7081 acgcatatag aaggctttaa tgtcttttgg atagtttttc acgaccaccg gcgatttaaa
     7141 gtgttcttcg gctaagaaac gctcgtgttc agaggaaaga tcgattcccc aagacaccgg
     7201 gaattcaaat tctttgccgg atttcaataa gatctcaatc gcatcggtgt aatctacttg
     7261 agcaaatggc gaattgataa agttttctaa acgactaatc gcttctttat caacgtgttt
     7321 aacgaaaaat tccatatcat ctttacgttc tgcaagcacc gctttaaaca catatttcag
     7381 catatcttcg gctaattttg cattgtcgtt taagtcggca aaggcaactt ccggctccat
     7441 catccagaat tctgcaagat ggcgagttgt gtttgagttt tctgcacgga atgtcggacc
     7501 gaaagtataa actttactta aggcacacgc ataggtttcc gcatttaact ggccggatac
     7561 ggttaagaac gattctttac cgaagaaatc ttggttaaaa tccactttgc cgttttcgcc
     7621 acgaggtaga ttttctaaat ccagcgtaga aacacggaac atttcacctg caccttcagt
     7681 atctgaggcg gtaattacag gtgttgctac ccaatagaaa ccttgctcgt ggaagaaacg
     7741 gtggatagct tgagctaaac agtgacgaac acgagcaact gcaccgatta agttcgtacg
     7801 agggcgtaag tgagcgactt cacgcagata ttcgatagag tggcgtttcg ctgccattgg
     7861 gtaggtatca ggatcttcca cccaaccaag cacttccact ttacttgctt gtaattcaac
     7921 ggcttgacct tcagcagggg attccacaac tttaccggtc accaccacag agcaacctgc
     7981 ggttaaatgt aatacttcgg cattataatt tggtagatcg ttattaataa tcgcttgaat
     8041 tgggtcaaag caagaaccat cgtaaacgct caagaaagat aaaccggctt ttgaatcacg
     8101 gcgggtacgc acccaaccac gcactgtcgc ttcttcacct accgctatct tgccggatag
     8161 gatttccgaa actgttggaa atttagacat tgaatacctc aaattctttg ttgtgagttt
     8221 taatataaga aacgttccgt attctacaag aaaatagggg gaaatataat aattttac
//
LOCUS       DANLRK010000114         8217 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.114, whole genome shotgun sequence.
ACCESSION   DANLRK010000114 DANLRK010000000
VERSION     DANLRK010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8217
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.114"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            177..554
                     /locus_tag="PXA33_001840"
     CDS             177..554
                     /locus_tag="PXA33_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HDL5121149.1"
                     /translation="MEYQFTHSIHGVVAKCSMDHEAFARWLNAEITENPSQLAPIFAE
                     IEKCRAAFPNHYECVFEGREYSLYFDYDEVMAKANNLDAAFDDEEMEEGFQFYNEESI
                     AFCGLDDFEKFLKAYQHFVQTYH"
     gene            643..1230
                     /gene="yihI"
                     /locus_tag="PXA33_001841"
     CDS             643..1230
                     /gene="yihI"
                     /locus_tag="PXA33_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HDL5121150.1"
                     /translation="MSRTKKTRRITDIMPARKTDKPKQPMPKLGGGKNRKLTRYELDA
                     QAREEKRKRKHKGLPTGSRNADPAEQKKAVVKEVKDPRIGSRKKVPLMVEFVNQPEKG
                     RTIKAVPVEPIKPTLSPEQELEQLENNECLNQLLDDLEAGKTLSAEDQKFMNECLDRI
                     DELMTELGIEYEDEEGDNGDALLRQFETMDINKFR"
     gene            1244..1711
                     /locus_tag="PXA33_001842"
     CDS             1244..1711
                     /locus_tag="PXA33_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2489 domain-containing protein"
                     /protein_id="HDL5121151.1"
                     /translation="MLRIFLMVLAALILISLGGYALHLMLKVRAQKKHEQALIEQAKQ
                     AQKDRYIRILDSIEVIARAMISEQCDFSEGVLRLKPLLDVLGRKLSDYTAMWALYGVV
                     ENMPILDERKNLKRNERMKLDLEREAKEAELESDIKNECYQLLKDIEEMRKAI"
     gene            1711..3078
                     /gene="hemN"
                     /locus_tag="PXA33_001843"
     CDS             1711..3078
                     /gene="hemN"
                     /locus_tag="PXA33_001843"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HDL5121152.1"
                     /translation="MSEIIWDLALIQKYNHSGPRYTSYPTALEFNENYTNEDFIRAAA
                     RYPERPLSLYVHIPFCHKLCYFCGCNKVITRHRHKVEIYLDYLEREIKTRAPLFQNRL
                     VTQIHWGGGTPTYLDEDQSARLMQMLRANFDVSDDAEISIEMDPREIELSMLDHLRAI
                     GFNRLSMGIQDFDKEVQRLVNREQDEEFIFALMKRAKELGFVSTNVDLIYGLPKQNVE
                     SFMYTLKRVVELNPDRMSVFNYAHLPSRFAAQVKIKEELLPAPETKLTILQNSIEFLG
                     KNGYKFIGMDHFAKPDDELAIAQEKGILHRNFQGYTTQEECDLLGMGVSAISLLGDTY
                     AQNRKELQQYYADVETNGIALHKGIALTQDDCIRRDVIKALICNFKLDFATIEKQYGI
                     DFKRYFAEDLAFLAPLAEDELLTMNDDSIVVSPRGRLLIRNICLCFDVYSRQLARRQQ
                     FSRII"
     gene            3152..5485
                     /gene="lptD"
                     /locus_tag="PXA33_001844"
     CDS             3152..5485
                     /gene="lptD"
                     /locus_tag="PXA33_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HDL5121153.1"
                     /translation="MKQRYSLLSLAVMAACYSNSAAADLKQQCLLGVPHFQGEVVEGD
                     QLRMPVTIEADSAVINQPRDATYTGDVTIKQGNRSIWADQVRVEQDGENARMAYLQGN
                     FNYQDNLIQATGSDATINLLSKDANLANANYQLIGRQGRGTAESGEFGNSKHTLKNAS
                     FTSCLPNDNSWQIEANEMIQHVDEEYAEMWHARFKVLGMPVFYSPYLQFPIGDRRRSG
                     LLIPSFERSSKNGWTYSQPFYWNIAPNMDSTITPTYYSRRGWQISPEFRYLTKIGQGL
                     VATEYMAKDRLSEYQPGNDDRKRYLFHWRHAMSFLTDWRFSVDYTKVSDRRYFSDFDS
                     DYGSSTDGYATQNFKLGYYQPNYNISISGKKFQTFDELDIGPYRVLPQIDFNYYKNDL
                     IKGGDFKFFAQAARFENDSKLMPKAWRFHAEPTLNFPLANRYGSLNLETKLYATYYQQ
                     TKGSESTEEIKKSVSRVLPQIKLDFKTVLESDKQLFSGYTQTLEPRVQYIYRPYKDQS
                     DIGSKAHRSVSLGYDSALLQNDYFSLFNDRRYSGLDRISSANLVTAGGTTRFFKEATG
                     EEVFNFSLGQTYYISPSKIDDTSDNSTKGHTTSWAAESNWKFHKNWNWHGSYQYDTRL
                     NQTALANMSLQYKPQSDKLIQLNYRYASKDYINQNLNTNKYGQSIKQVGGVLGWELTD
                     KVSVMTSYYHDLALKKPVEGQISLNYNTCCWAANVYVARRLVSTPVGSSDTINDFYYD
                     NKFGINFELRFGTNYSNGVTRMLKRGIIPYTEPYSIN"
     gene            complement(5627..>8217)
                     /gene="ppc"
                     /locus_tag="PXA33_001845"
     CDS             complement(5627..>8217)
                     /gene="ppc"
                     /locus_tag="PXA33_001845"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HDL5121154.1"
                     /translation="FLGETIRDAQGNYILELIESIRVLSRNSRAGDENAREQLLDKLA
                     NISNDDVLPVARAFSHFLNLTNIAEQHQTVSRHHIDESLGSRSLDSLFLRLKSQNIPV
                     EKVIKTVESLMIDLVLTAHPTEVTRRSLGHKHVEINKCLNLLEHDDLTEAESYKIKRR
                     LMQLIALAWHTNEIRTQRPTPVDEAKGGMAVIENSLWKAVPEFCRQLNFYLEKHFGVQ
                     YPVNLAPVRFSSWMGGDRDGNPFVTAETTRRVLRMNRWKATELFLQDIKNLAEELSIT
                     QCTPAFRAKYGNHLEPYRVLLKVLRQKLINTEIYYAELLEGKISTIDESDILTSDQQL
                     WEPLFDCYESLQACGMRIIANGALLDCLRRINCFGLGLSRLDIRQESTRHEMAIAEIT
                     RYIGLGDYAHWTEDDKQAFLVRELSSRRPLIPTNWTPSPETKEVLDTCKIVAEQPEGV
                     ISAYVISMAREASDVLSVHLLLKEAGCKFTIPVAPLFETLGDLDHSEKVMTDLFNIGW
                     YRGVINNYQMIMIGYSDSAKDAGMLAAGWAQYRAQEALVNLCEKYNIELTLFHGRGGT
                     IGRGGAPAHAALLSQPPRSLKNGLRVTEQGEMIRFKLGLPAVAVESLELYASAILEAN
                     IFPPPEPKQQWRDVLAKASDISCQIYRDMVRGELDFVPYFRSTTPEQELSRLPLGSRP
                     AKRNPKGGVESLRAIPWIFAWMQNRLMLPAWLGAGQALQQLIDEGNETLLKEMCNEWP
                     FFSTRIGMLEMVFSKADIWLAEYYDQRLVEPKLHRLGNMLRTQLQADLKTVLALAHDG
                     QLMADLPWIAESIALRNIYTDPLNLLQVELLDRIRKQGENADPVLEQALMITITGIAA
                     GMRNTG"
BASE COUNT         2487 a         1594 c         1810 g         2326 t
ORIGIN      
        1 gtagttcctt tttgatgtat caatactaaa aataacctgt attattctag cagatttctg
       61 ggtaatcgag gagttgataa catttaaaaa ttagcgtttt gcaaaatttt ccaaaaaaag
      121 accgcttgtt aggctataat agccaaccaa acagaccaat tcaaaggaga atcggtatgg
      181 aatatcaatt tacccatagt attcacgggg tggtggcaaa atgttcgatg gatcacgagg
      241 cgtttgctcg ttggttgaat gccgaaataa cggaaaatcc tagccagtta gcccctattt
      301 tcgctgaaat agagaagtgt cgagccgcat ttccgaatca ttatgaatgt gtgtttgaag
      361 ggcgggagta tagtctctat tttgattatg atgaagtgat ggcaaaagca aacaatttag
      421 atgctgcttt tgatgatgag gaaatggaag aaggctttca attttacaat gaagagagca
      481 tcgcattttg tggcttagat gactttgaga aatttttgaa agcctaccaa cattttgtgc
      541 aaacttatca ttgattgaaa tgattaattg aaaccaaagc tattaaggga atctaattcc
      601 ttgtagaata gccgtttata gcaattttaa gatattaaat ttatgagtcg tactaaaaaa
      661 acacgtcgta ttaccgacat tatgccggca agaaagaccg ataaacctaa acagcccatg
      721 cctaaattag gtggggggaa aaatcgcaaa ctgacccgtt atgagttaga tgctcaagcc
      781 cgtgaggaaa aacgtaagcg taaacataaa ggcttgccga caggctcacg caatgctgat
      841 ccggcagagc agaaaaaagc agtggtgaaa gaggtgaaag atccgcgtat cggtagccgt
      901 aaaaaagtgc cgttaatggt ggaatttgtg aaccagccgg aaaaagggcg gacgattaag
      961 gctgtgccgg ttgagccaat caaaccaacg ctttcaccgg aacaagagct ggagcagtta
     1021 gaaaataacg aatgtttaaa tcagttgtta gatgatttgg aggcgggcaa aaccttatcc
     1081 gcagaagatc agaaatttat gaatgaatgt ttagatcgca ttgatgaatt aatgactgag
     1141 cttggcatcg aatatgaaga tgaagagggc gataatggcg atgcgttatt acgccaattt
     1201 gaaacgatgg atattaataa attccgctag gtagccgatt atgatgctaa gaatattcct
     1261 gatggtgctt gccgctttga ttttaatttc gctgggcggg tatgcactgc acttaatgct
     1321 gaaagtgcga gctcagaaaa agcacgaaca agcactgatt gaacaggcaa agcaagcaca
     1381 aaaagatcgt tatattcgga ttttagacag cattgaagtt attgctcggg caatgatttc
     1441 agaacagtgc gatttttcgg aaggcgtttt acgtttaaaa ccgctattag atgttctcgg
     1501 cagaaaatta agcgactata cggcgatgtg ggcgttgtat ggcgtggtag aaaatatgcc
     1561 gattttagat gaacgcaaaa atttaaaacg taacgaaaga atgaagttgg atttagagcg
     1621 ggaagcaaaa gaagcggagc ttgaatcaga catcaaaaat gagtgttacc aattattaaa
     1681 agatattgaa gaaatgagaa aggcaatcta atgtcagaaa ttatttggga tttagccctt
     1741 attcaaaaat ataaccattc agggcctcgt tatacctctt acccaacagc gttggagttt
     1801 aacgaaaatt acaccaatga agattttatt cgtgctgcag ctcgttaccc tgagcgtccg
     1861 ctttcgcttt atgtgcatat tccgttttgc cataaactgt gctatttctg tggctgtaat
     1921 aaagtgatta ctcgtcatcg ccataaagtt gaaatttatt tagattattt agagcgtgaa
     1981 attaaaactc gcgcacctct gtttcaaaat cgtttagtga cccaaataca ctggggtggc
     2041 ggtacgccga cttatttaga cgaagatcaa tcggcacgtt taatgcaaat gctacgagcc
     2101 aattttgatg tgagcgatga tgcggaaatc agcattgaaa tggatccgcg tgaaatcgaa
     2161 ttaagtatgt tggatcacct gcgtgctatc ggttttaacc gtctgagtat gggtattcag
     2221 gatttcgata aagaagtgca acgtttggtg aatcgtgagc aagatgaaga gtttattttt
     2281 gccttgatga aacgggcaaa agaacttggt tttgtttcaa ctaatgtgga tttgatttac
     2341 ggcttgccaa aacagaatgt ggaaagcttt atgtacacct taaaacgtgt ggtagagcta
     2401 aaccctgatc gtatgagcgt atttaactat gcccacttgc caagccgttt tgctgcacaa
     2461 gtgaaaatta aagaagagtt gttgccggct ccggaaacca aattgacgat tttgcaaaat
     2521 tccatcgaat ttttaggcaa aaacggttat aagtttatcg gtatggatca ctttgctaaa
     2581 cctgatgatg agttagctat tgcacaagaa aaaggcattt tacaccgtaa cttccaaggc
     2641 tataccactc aagaagagtg tgatttattg ggaatgggcg tatcggcaat cagcttactc
     2701 ggcgatactt acgcccaaaa ccgcaaagaa ttacaacaat actatgctga tgttgaaacc
     2761 aacggtattg cgttgcataa aggcattgcg ttaacccaag atgattgcat tcgccgtgat
     2821 gtgataaaag ccttgatttg taatttcaaa ttagattttg ccaccattga aaagcaatat
     2881 ggtattgatt ttaaacgtta ttttgccgaa gatttagctt tcttagcgcc gttggcggaa
     2941 gatgaattat taacgatgaa tgacgacagc attgtggttt ccccacgagg gcgtttattg
     3001 atccgtaata tttgcttatg ctttgatgtc tattcaagac aattagcgag aagacagcag
     3061 ttttcccgta ttatttaatg agcaagcggt cgattttggt aaaaattttg caaaaacaga
     3121 ccgcttgttc ccggcttata aaggaaaaat gatgaaacag cgttatagct tactttctct
     3181 tgctgtgatg gcagcttgtt atagcaacag tgccgcagct gatttaaagc aacaatgttt
     3241 acttggcgtg ccgcattttc agggcgaagt ggtagagggc gatcaactta gaatgcctgt
     3301 taccattgag gctgacagtg cggtcattaa ccagcctcgt gatgccacct acaccggtga
     3361 tgtaacgatc aaacaaggta accgttcgat ttgggcggat caggtgcggg ttgagcaaga
     3421 tggcgaaaat gcccgaatgg cgtatttgca gggtaatttt aactaccaag ataatttgat
     3481 tcaggcaacc ggttcagatg cgaccatcaa tctgctttcc aaagatgcga atttagctaa
     3541 tgctaattac caattaattg gcagacaagg gcgtggcacg gcagaatccg gtgaattcgg
     3601 caacagcaaa cacacgctga aaaatgccag tttcacttcg tgcttaccga atgataattc
     3661 gtggcaaatt gaagcgaatg aaatgattca gcacgttgat gaagaatatg cggaaatgtg
     3721 gcacgcccgt tttaaagtgc tgggaatgcc ggtattttat tcgccttact tacaatttcc
     3781 gattggcgac cgccgccgta gtgggttatt aattccaagt tttgagcgtt caagcaaaaa
     3841 cggctggaca tattcgcaac cgttttattg gaatatcgca ccgaatatgg actcgaccat
     3901 cactccaact tactattcac gccgtggttg gcaaatcagc ccggaatttc gttatttaac
     3961 gaaaatcggg caaggcttag tggcaaccga atatatggcg aaagatcgtt taagcgagta
     4021 ccaaccggga aatgatgatc gtaaacgcta tttattccac tggcgtcacg caatgagttt
     4081 tttaaccgat tggcgtttct cggtcgatta caccaaagtg agcgacagac gttatttctc
     4141 tgattttgat tcggattatg gtagcagtac tgatggctat gcgacgcaga attttaaatt
     4201 aggctactat cagccgaatt ataatatctc aatttcaggt aaaaaattcc aaaccttcga
     4261 tgagttggat atcgggcctt atcgcgttct gccacaaatt gattttaact attataaaaa
     4321 cgatctaatt aaaggcggag atttcaaatt ctttgcccaa gcagcccgtt ttgaaaatga
     4381 tagtaaatta atgccaaaag cgtggcgttt ccacgctgag ccaacactga atttcccatt
     4441 agcgaaccgc tatggtagct taaatttaga aaccaagctc tatgcaacct actatcaaca
     4501 aacgaaaggt agtgagagta cggaagaaat taagaaaagt gtaagtcgtg tactgccaca
     4561 gattaagctc gattttaaaa ccgtgttgga atccgataaa cagcttttct ctggctatac
     4621 tcaaacgctt gagcctcggg tgcaatatat ttatcgccca tataaggatc aaagcgatat
     4681 tggctcgaaa gcacatcgaa gtgtgagctt aggctatgac tctgcattat tgcaaaacga
     4741 ctatttctca ctgtttaatg acagacgtta tagcggttta gatcgtattt cgtcagcaaa
     4801 tttagtcacc gcaggtggca caacccgttt ctttaaagag gcaaccggtg aagaggtgtt
     4861 taatttcagc ttaggacaaa cttattatat aagcccgtct aaaattgatg atacctcaga
     4921 taacagcact aaaggtcata cgacttcatg ggcagcggaa tctaactgga aattccataa
     4981 gaattggaat tggcatggta gctatcaata tgatactcgt ttaaaccaaa cggcgttagc
     5041 gaatatgtca ttgcaataca aaccacagtc agacaaattg attcagctta attaccgcta
     5101 tgcaagtaaa gattacatca atcaaaactt gaacacgaat aagtatgggc aatccattaa
     5161 acaagtgggc ggtgtgctag gttgggaatt aaccgataaa gtgtcggtaa tgaccagtta
     5221 ttaccacgac cttgcattga aaaaaccggt ggaagggcaa atttcgctta actacaacac
     5281 ttgctgttgg gcggcaaatg tgtatgtggc tcgtcgctta gtttcaacgc cggtaggttc
     5341 atctgatacg atcaatgatt tctactatga caataaattc ggcattaact ttgagctacg
     5401 ctttggcaca aactacagca atggtgtaac ccgtatgcta aaacgaggta ttattcctta
     5461 caccgagccg tatagcatta attaacaaag taaaaacaaa taagtaaaaa tacaaatcgg
     5521 ggcgaaaaat atttgccccg atttttttat atttggctga taagcggtta aattctgttg
     5581 gaattttgca aatcttccgc taaattcaac cgcttgccat cgaaacttaa cctgtatttc
     5641 gcatacctgc ggcaatacct gtgatggtga tcatcaatgc ttgctctagt acaggatcag
     5701 cattttcacc ttgtttgcga atcctatcta acaattctac ctgaagtaga ttgagcggat
     5761 cggtataaat attgcgtagt gcaatcgatt ctgcgatcca aggtaaatcg gccattaatt
     5821 gtccatcgtg agccagtgca agtacggttt taagatcagc ctgtagttgc gtacgcaaca
     5881 tattgcctaa acggtgtagt tttggctcga ctaggcgttg gtcgtaatat tccgccagcc
     5941 aaatatcagc tttactaaat accatttcca gcattccgat acgggtcgag aaaaacggcc
     6001 attcgttaca catttctttt agtaaggttt cattaccttc atcaatcaac tgttgcaatg
     6061 cttgccctgc acctaaccaa gcaggtagca ttaagcggtt ttgcatccaa gcgaaaatcc
     6121 aaggaattgc acgcaaactc tccactccgc cttttggatt acgttttgca gggcgtgagc
     6181 caagcggcag acgggaaagt tcttgctctg gtgtagtaga gcggaaatac ggtacgaagt
     6241 ctagctcgcc acgcaccata tcacggtaaa tttggcaaga aatatcggag gcttttgcta
     6301 gcacatctcg ccattgctgc tttggctccg gtggtgggaa aatattcgct tctaaaatcg
     6361 cactggcata gagttctaaa ctttcaactg ccacggcagg taagcctaat ttaaagcgga
     6421 tcatttctcc ttgttcggtc actcgtaagc catttttgag tgagcgaggt ggctgagaaa
     6481 gtaaggctgc gtgagccggt gcgccaccac gcccgatagt accaccacga ccgtggaata
     6541 aggttaattc aatattatat ttttcacata aattaaccag agcttcttgc gcccgatatt
     6601 gcgcccagcc ggcagcaagc attccggcat ctttggcgga atcagagtag ccgatcataa
     6661 tcatttggta attgttgatg acgccacgat accagccgat attaaataaa tcggtcatca
     6721 ctttttcact atgatctaaa tcgcctagcg tttcaaataa tggggcaacc ggaatagtaa
     6781 atttgcaccc cgcctctttg agtaaaaggt gaaccgagag tacatcagag gcctctcttg
     6841 ccattgaaat gacataggct gaaatgacac cttcaggttg ctcggcaaca attttacaag
     6901 tgtctaacac ttctttagtt tcagggcttg gagtccagtt ggttggaatc aatgggcggc
     6961 gagaactgag ttcacgcact aagaatgcct gtttatcatc ttccgtccag tgagcataat
     7021 cgcctaagcc gatatagcgg gtaatttctg caatcgccat ttcgtggcgg gtactttctt
     7081 ggcgaatatc taagcgtgat aaacctaaac caaaacagtt tatacgacgt aagcaatcta
     7141 gtaatgcacc atttgcaata attcgcatac cacaggcttg taatgactca tagcaatcaa
     7201 aaagcggttc ccacaattgc tgatctgaag ttaaaatgtc actttcatca atagttgaaa
     7261 ttttaccttc caataattcc gcatagtaaa tttcggtatt aattaatttc tggcgtaata
     7321 cttttagtaa tacgcgatat ggctctaaat ggttgccata tttagcacga aatgcagggg
     7381 tacactgtgt aatcgataat tcttcagcaa gatttttaat atcttgtaag aacaattccg
     7441 tggctttcca acggttcatt cttaatacac ggcgagttgt ttctgccgtg acgaacgggt
     7501 taccgtcacg gtctccgccc atccaagaag aaaaacggac aggggctaaa tttaccggat
     7561 attgcacacc aaagtgtttt tctaaataga agttaagctg acggcaaaat tccggcacgg
     7621 ctttccataa actattttca atgactgcca taccgccttt ggcttcatca acaggggttg
     7681 ggcgttgggt tctgatttca ttagtatgcc aagcaagggc aatcagttgc attaaacggc
     7741 gcttgatttt atagctttct gcctcagtta aatcatcatg ttccaacaaa ttcaaacatt
     7801 tattgatttc aacgtgttta tgccccagcg aacggcgggt tacttccgtt gggtgagctg
     7861 ttagcactaa atcaatcatt aggctttcca cggttttaat cactttttca acggggatat
     7921 tttgtgattt tagacgaagg aacagagagt ctaatgagcg actaccaagt gattcatcaa
     7981 tatgatggcg agaaacagtt tgatgttgtt cagcaatatt tgttagattt agaaaatggc
     8041 taaatgcacg tgcaacgggt aagacatcat cgttagaaat attggctaat ttatctaata
     8101 gctgttcacg ggcgttttca tcgccggcac gagaattgcg agataacaca cgaatgcttt
     8161 cgatgagttc tagaatgtaa tttccttgcg catcacgaat cgtttctccc aaaaatt
//
LOCUS       DANLRK010000115         8123 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.115, whole genome shotgun sequence.
ACCESSION   DANLRK010000115 DANLRK010000000
VERSION     DANLRK010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8123
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.115"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            <1..920
                     /locus_tag="PXA33_001846"
     CDS             <1..920
                     /locus_tag="PXA33_001846"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015701738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HDL5121155.1"
                     /translation="KLGIIEVQGPILSEVGNGMQDNLSGIEKAVKVNVKCIPSATYEV
                     VHSLAKWKRHTLARFGFKEGEGLFVHMKALRPDEDSLDRTHSVYVDQWDWEKVIPEGR
                     RNFDFLKETVNEIYKAIRLTELAVEARFDIPSVLPKQITFVHSEDLVQRYPNMTSKER
                     ENAICKECGAVFLIGIGGKLSDGKPHDGRAPDYDDWTTESEQGYKGLNGDILVWNEEL
                     GTAFELSSMGIRVDETALRLQVALTGDEDRLEMDWHKDLLAGRLPLSIGGGIGQSRLV
                     MFLLRKKHIGEVQSSVWPKAVFEEFENIL"
     gene            complement(1044..1463)
                     /gene="atpC"
                     /locus_tag="PXA33_001847"
     CDS             complement(1044..1463)
                     /gene="atpC"
                     /locus_tag="PXA33_001847"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HDL5121156.1"
                     /translation="MASQFELTVVSAERKIFEGTVTSVRVSGTDGELGVYAGHAPLLT
                     AIKPGMVKFTLADGKEEFIYVSGGFLEVQPTVVTVLADTAIRGEELDEQRILAAKRKV
                     EETLSKTSDADMTAKLAREIAKLRVYELTKTKLANKR"
     gene            complement(1491..2864)
                     /gene="atpD"
                     /locus_tag="PXA33_001848"
     CDS             complement(1491..2864)
                     /gene="atpD"
                     /locus_tag="PXA33_001848"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011978979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HDL5121157.1"
                     /translation="MATGKIVQIIGAVIDVEFPQDAVPKVYDALKVESGLTLEVQQQL
                     GGGVVRCIALGSSDGLKRGLKVENTNKAIEVPVGTKTLGRIMNVLGEPIDEAGPIGEE
                     ERWTIHRAAPSYEEQANSTELLETGIKVIDLVAPFAKGGKVGLFGGAGVGKTVNMMEL
                     IRNIAIEHSGYSVFAGVGERTREGNDFYHEMKDSNVLDKVSLVYGQMNEPPGNRLRVA
                     LTGLTMAEKFRDEGRDVLFFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEMGVL
                     QERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDSTVVLSRNIASLGIYPAVDP
                     LDSTSRQLDPLVVGEEHYNVARGVQGTLQRYKELKDIIAILGMDELSEDDKRLVARAR
                     KIERFLSQPFHVAEVFNGVPGKFVPLKETIRGFKGILEGEYDHIPEQAFYMAGSIDEV
                     VERAKQM"
     gene            complement(2885..3751)
                     /gene="atpG"
                     /locus_tag="PXA33_001849"
     CDS             complement(2885..3751)
                     /gene="atpG"
                     /locus_tag="PXA33_001849"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011201492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HDL5121158.1"
                     /translation="MAGAKEIRTKIASVRNTQKITKAMEMVAASKMRKTQERMAASRP
                     YAESIRKAISHIAKGNIEYKHPFLTPRPVKKVGYLVVSTDRGLCGGLNINLFKTVLHE
                     LKEKDDQGVKSRLAVVGNKGISFFNPMGLEIKGHINGLGDTPAMEDLVGIVNGMVNAY
                     REGEIDEVYVVYNRFINTMSQKPTVQQLLPLPALENDSLEQTGSWDYLYEPNPQALLD
                     SLLVRYLESQVYQAVVDNLASEQAARMVAMKAATDNAGNLINELQLVYNKARQASITN
                     ELNEIVAGAAAI"
     gene            complement(3777..5318)
                     /gene="atpA"
                     /locus_tag="PXA33_001850"
     CDS             complement(3777..5318)
                     /gene="atpA"
                     /locus_tag="PXA33_001850"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HDL5121159.1"
                     /translation="MQLNSTEISELIKKRIAQFNVVSEAQSTGTIVSVSDGVIRIHGL
                     SDVMQGEMIALPGNRYAIALNLERDSVGAVVMGPYADLAEGMQVQCTGRILEVPVGRG
                     LLGRVVNTLGQPIDGKGEIQNDGFSPIEVIAPGVIDRQSVDQPVQTGYKAVDSMVPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTIANVVRKLEEHGAL
                     ANTIVVVASASESAALQYLAPYAGCAMGEYFRDRGEDALIVYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERASRVNADYVERFTNGAVKGQTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLESGLFNAGIRPAVNPGISVSRVGGSAQTKLVKKLAGG
                     IRTALAQYRELAAFAQFASDLDEATRKQLSHGQKVTELLKQKQFEPMSVAQLGLSLAA
                     AEYGYLDDIPVERVGSFEANLLAYAQSNYGEFMQELSKSGNFNDEIKDKLNEILSGFK
                     KNSAW"
     gene            complement(5331..5864)
                     /gene="atpH"
                     /locus_tag="PXA33_001851"
     CDS             complement(5331..5864)
                     /gene="atpH"
                     /locus_tag="PXA33_001851"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HDL5121160.1"
                     /translation="MSELSTVARPYAKAAFDFALEQGQLEQWQQMLTFSAAVANDEQV
                     ASFLNSSLASGQISETFIKICGEQLDQYGQNFIRVMADNKRLAVLPAVLNAFLELRAE
                     QESIKDVEVVSADKLTDAQEAKIANAMEKRLGTKVRITSSIDSSLIAGVIIRYDDVVI
                     DGSSRGQLNRLSQELSL"
     gene            complement(5878..6348)
                     /gene="atpF"
                     /locus_tag="PXA33_001852"
     CDS             complement(5878..6348)
                     /gene="atpF"
                     /locus_tag="PXA33_001852"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HDL5121161.1"
                     /translation="MNLNATLIGQLIAFALFVAFCMKFVWPPLIRVIEERQANIASAL
                     ASAEKAKQEQADSKAAADQEILRAKEEAQNIIDLATKRRNEILESVQAEAEVERQRII
                     EQGYAEVESERKRVQEELRQKVAALAIAGAEKIVGRSVDAAANNDIIDKLVAEL"
     gene            complement(6409..6663)
                     /gene="atpE"
                     /locus_tag="PXA33_001853"
     CDS             complement(6409..6663)
                     /gene="atpE"
                     /locus_tag="PXA33_001853"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HDL5121162.1"
                     /translation="MESVITATIIGASILLAFAALGTAIGFAILGGKFLESSARQPEL
                     ASSLQTKMFIVAGLLDAIAMIAVGISLLFIFANPFIDLLK"
     gene            complement(6781..7569)
                     /gene="atpB"
                     /locus_tag="PXA33_001854"
     CDS             complement(6781..7569)
                     /gene="atpB"
                     /locus_tag="PXA33_001854"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005713638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HDL5121163.1"
                     /translation="MAGTTAEYISHHLSFLSTGEGFWAVHLDTLFFSLVAGAIFLWVF
                     SRVAKNATTGVPGKLQCFVEIVIEWVNGLVKDNFHGPRNVIAPLALTIFCWVFIMNAI
                     DLIPVDFLPQLANLFGIHYLRAVPTADISATLGMSICVFALILFYTVKSKGFGGLVKE
                     YTLHPFNHWAFIPVNFILETVTLLAKPISLAFRLFGNMYAGELIFILIAVMYMADNFA
                     LQALGIPLHLVWAIFHILVITLQAFIFMMLTIVYLSIAYNKADH"
     gene            complement(7592..7975)
                     /locus_tag="PXA33_001855"
     CDS             complement(7592..7975)
                     /locus_tag="PXA33_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HDL5121164.1"
                     /translation="MSAVINRAKSQYKQALKVEFFLLCVFALGVSLWKMNFNFSFLGG
                     ALASFLPHLVFVYWVFFKKSAKNQSKMTAFYRGEGMKWLVTIILVIGCFKLIPALDVV
                     LFFVGYFIALFLNNMIPFVLSKHHH"
BASE COUNT         2400 a         1837 c         1526 g         2360 t
ORIGIN      
        1 ataaattagg cattattgaa gttcaggggc ctattttgag cgaagtcggc aacggtatgc
       61 aagacaacct ttccggtatc gaaaaagcag taaaagtaaa cgtaaaatgt attccaagcg
      121 ctacttatga agtggttcat tctcttgcaa aatggaaacg ccacacctta gctcgttttg
      181 gatttaaaga aggagaaggg ttatttgttc atatgaaagc tcttcgccct gatgaagatt
      241 ctttagatcg cacacactcg gtatatgttg accaatggga ctgggaaaaa gtcattcctg
      301 aaggtcgccg caacttcgat ttcttaaaag aaacggtaaa cgaaatctat aaagcaattc
      361 gtttaaccga attagcggtg gaggctcgtt tcgatattcc atctgtattg cctaaacaaa
      421 ttactttcgt tcacagcgaa gacttagtgc aacgctatcc gaatatgacc agcaaagagc
      481 gtgaaaatgc tatctgtaaa gagtgcggag cggtgttctt aatcggtatc ggcggcaaat
      541 tatcagacgg taaacctcac gatggacgag ctccggacta tgatgactgg acaaccgagt
      601 ccgaacaagg ctacaaaggc ttaaacggcg atattttagt ttggaacgaa gaactcggca
      661 cagcctttga actttcctca atgggtattc gtgtagatga aaccgcctta cgcttacaag
      721 ttgctttaac tggcgacgaa gaccgcctcg aaatggattg gcacaaagat ttattagccg
      781 gcagactccc gctttctatc ggcggcggta tcggtcaatc tcgcttagtg atgttcttac
      841 tacgcaaaaa acacatcggt gaggtgcaat ccagtgtctg gccaaaagcg gtatttgaag
      901 agtttgagaa tattctttaa taaaacaaaa aagcgaacct tagggttcgc ttttgctttc
      961 tacaagcggt cttattttgc aaaaaattta ctgtttttaa ccgcttgtaa aacaaaaccc
     1021 cactttcgtg gggttttttg atattaacgt ttattcgcta atttggtttt agtgagttcg
     1081 taaacacgca gtttcgcaat ttctctcgct aacttcgcgg tcatatccgc atcgctggtt
     1141 tttgaaagtg tttcttctac tttgcgtttt gcggcaagaa tacgctgttc gtctagttct
     1201 tcaccacgaa ttgcggtatc agccaacact gttactacgg tcggttgtac ttcaaggaaa
     1261 ccacctgaaa cgtagataaa ctcttctttg ccgtcagcta aagtgaattt aaccattccc
     1321 ggtttaattg cggttaataa cggtgcatgc cctgcataca cccctaattc accatctgtc
     1381 cctgaaacac gcacgctggt aactgtgcct tcaaagattt tacgttctgc actcactacc
     1441 gtgagttcaa attgagatgc catgttgttc tcctttagtt tagaacaaaa ttacatctgt
     1501 tttgctcttt caaccacttc gtcaattgaa cccgccatat agaacgcttg ttccggaata
     1561 tggtcgtatt cgccttctaa aatgccttta aagccacgaa ttgtttcttt taacggtacg
     1621 aatttgcccg gtacgccgtt gaatacttcc gcaacgtgga atggttgtga aaggaaacgt
     1681 tcgattttac gcgcacgagc aacaaggcgt ttgtcgtctt ccgataattc atccataccc
     1741 aaaattgcaa tgatgtcttt taactcttta taacgttgta atgtaccttg tacaccacga
     1801 gcaacgttat agtgttcctc accaaccacg agtggatcta actgacgaga ggttgagtca
     1861 agagggtcaa ccgccgggta aataccgagt gaagcgatat tacggcttaa taccaccgtt
     1921 gagtctaagt gggcaaaggt agttgccgga gacgggtcag ttaagtcatc cgccggaaca
     1981 tataccgctt gcacagaggt aatagagccg gttttggttg aggtaatacg ctcctgtaat
     2041 acgcccatct cttctgcaag agtcggctga taacctaccg cagacggcat acgacctaat
     2101 aacgcagata cttcggtacc agctaaagta taacggtaga tgttatccac gaagaataat
     2161 acgtcacgac cttcatcacg gaatttttct gccatggtta agccggttaa tgccacacgc
     2221 agacggttgc ccggtggttc gttcatttga ccgtaaacca gcgatacttt gtctaatacg
     2281 ttagagtctt tcatttcgtg gtagaagtca ttaccctcac gagtacgctc acctacaccg
     2341 gcgaataccg agtaaccgct atgttcgata gcgatgttac ggattaactc catcatattt
     2401 acggttttac ctacacccgc accaccgaat aagccaactt taccaccctt agcaaatgga
     2461 gcaactaagt cgataacttt gataccggtc tctaaaagct cggtgctgtt cgcttgttct
     2521 tcgtagcttg gtgctgcacg gtggattgtc caacgttctt cttcaccgat tgggcctgct
     2581 tcatcaattg gctcacctaa tacgttcata atacgaccga gtgtttttgt accaaccggt
     2641 acttcaatcg ctttattcgt attttcaact tttaagccac gttttaaacc atcagatgaa
     2701 ccaagtgcga tacaacggac cacaccgcca cctaattgtt gttgaacttc aagggttaat
     2761 cctgattcaa cttttaatgc atcataaact tttggtactg catcttgcgg aaactcaaca
     2821 tcgattaccg caccgatgat ctgtacaatt tttcccgttg ccattaccgt tcctctattc
     2881 attgttaaat tgctgcggca cccgcgacaa tttcatttaa ttcattcgta atacttgctt
     2941 gacgagcttt gttatacact aactgtaact cattaatcag attacctgcg ttatcggttg
     3001 ctgctttcat tgccaccatt cgagcagcct gttcagacgc aagattatct accactgcct
     3061 gataaacttg agattctaaa taacgaacca gtaagctgtc taataacgct tgtggatttg
     3121 gttcatagag ataatcccaa gaaccagttt gctctaatga gtcattttcc agtgcaggca
     3181 aaggaagcaa ctgttgtact gtcggttttt gtgacatcgt gtttataaaa cggttatata
     3241 ccacatacac ttcatcaatt tcgccttcac ggtaggcatt taccatacca ttaacaatac
     3301 cgactaaatc ttccattgcc ggtgtatcac ccaatccatt gatatgacct ttaatctcta
     3361 gccccattgg gttaaaaaag gagatccctt tatttcccac cacagcaagt cgagacttaa
     3421 caccttggtc atctttttct ttcaattcat gtaaaacggt tttaaataaa ttgatattta
     3481 agccaccaca taaaccgcga tcggttgaaa ctactaaata gccaactttt tttaccggac
     3541 gtggggtcaa aaatgggtgt ttatactcaa tgttaccttt ggcaatatgg ctaattgcct
     3601 tgcgaatact ttcagcataa gggcgagaag ccgccatacg ctcttgggtt ttacgcattt
     3661 ttgatgcggc aaccatttcc atcgctttgg taattttttg tgtattacga acacttgcaa
     3721 ttttggttct tatctcttta gcacctgcca tattatttct ccgctaaaaa gttagattac
     3781 caagcactgt tctttttgaa accagataaa atctcgttca atttatcttt aatttcatcg
     3841 ttgaaattgc cggattttga cagttcttgc ataaactcgc cgtagttact ttgagcataa
     3901 gctaataagt ttgcttcaaa tgagccaaca cgctcaaccg gaatatcatc taagtaaccg
     3961 tattcagctg ctgctaatga taaacctaat tgtgcaacag acatcggctc gaattgtttt
     4021 tgtttcaaca attcagttac tttttgaccg tgtgaaagct gtttacgtgt tgcttcatca
     4081 agatctgatg cgaactgggc aaatgccgct aattcacggt attgagcaag tgcggtacgg
     4141 ataccaccgg caagcttctt aaccagttta gtttgtgcag aaccacctac acgggaaacc
     4201 gagatacccg ggtttaccgc agggcgaata ccggcattga ataagcctga ttctaagaaa
     4261 atctgaccat cggtaattga aattacgtta gtcggtacga aggctgaaac gtcacctgct
     4321 tgggtttcga tgatcggtaa tgcggttaaa gaaccggttt gacctttcac agcaccgtta
     4381 gtgaaacgtt ctacatagtc tgcatttaca cgagacgcac gctctaataa gcgagagtgt
     4441 agatagaaca catcacccgg gaatgcttca cgacccggtg gacggcgtaa taacaatgaa
     4501 atttgacggt aagcaactgc ttgcttagac aaatcatcgt aaacgatcaa cgcatcttca
     4561 ccacggtcac ggaagtattc acccattgca caacctgcat aaggtgcaag atattgtaat
     4621 gcagcagatt cagacgcaga agccactaca acgatagtgt tcgctaatgc accgtgttct
     4681 tctaatttac gcaccacgtt ggcgatagtt gatgcctttt gaccgattgc aacgtagata
     4741 catttaatgc ctgaatcacg ttggttgatg atcgcatcaa tcgctaatgc ggttttaccg
     4801 gtttgacggt cacctataat caattcacgc tgaccacgac cgattggtac cattgagtcc
     4861 accgctttgt aaccggtttg aaccggttga tcaaccgatt gacggtcgat aacgcccggt
     4921 gcgatcactt cgattggtga gaaaccgtca ttttggattt cgcctttacc atcgattggc
     4981 tgacctaagg tatttacaac acgacctaat aagccacgac caaccggcac ttctaaaata
     5041 cgaccggtac attgtacttg cataccttct gctaaatcgg cataaggtcc cattacaact
     5101 gcaccaacag aatctctttc taagtttaat gcaatcgcat aacgattgcc cggtaatgca
     5161 atcatttcac cttgcattac atcagataag ccgtggatac gaataacccc gtcacttacc
     5221 gaaacgattg tccctgtgct ttgagcttca ctcaccacat taaactgggc aatacgtttt
     5281 ttaatcaatt cactaatttc agttgaattt agttgcattt tttattcctc ttacaagctc
     5341 aactcttgac tcaagcggtt tagctgtcca cgactactac catcaatcac tacatcatca
     5401 taacgaatga tcacacccgc aattaatgaa ctatctattg atgaagtaat acgtactttc
     5461 gttccgagtc gcttttccat cgcattggca atttttgctt cttgtgcatc tgttaattta
     5521 tctgcagaaa caacctcaac atctttaatt gattcctgtt ctgctcttaa ttctaaaaat
     5581 gcattcaata ccgcaggtaa tactgctaaa cgcttgttat cagccattac gcgaatgaaa
     5641 ttttgcccat attgatctaa ttgttcgcca caaatcttga taaacgtctc agaaatctga
     5701 ccgcttgcaa gtgatgagtt aagaaaacta gctacttgct catcgttagc gacggcggca
     5761 gaaaaagtta acatctgctg ccattgttcc aattgacctt gttctaaagc aaaatcgaaa
     5821 gccgctttag cataggggcg agctactgtg cttaattctg acatctgccc ctcctagtta
     5881 tagttccgca actagcttat caataatatc gttgttcgca gcggcatcca ccgaacgacc
     5941 cacgattttt tctgcaccag caatagccaa tgcagccact ttttgacgaa gctcttcttg
     6001 aacgcgttta cgttcgcttt caacttccgc gtaaccctgt tcaataatac gttgacgttc
     6061 cacttcagct tctgcttgta cagattctaa aatttcatta cgacgttttg ttgctaaatc
     6121 aatgatattt tgtgcttctt ctttcgctct aaggatttct tgatccgcag ccgctttaga
     6181 atcagcttgt tcttgtttcg ctttttcggc agaagccaat gcgctagcaa tatttgcctg
     6241 acgctcttct atcactcgaa ttagcggtgg ccatacaaac ttcatacaga acgcaacaaa
     6301 aagtgcgaac gcaatcagtt gaccaattag tgttgcattt aaattcacaa cgccctccta
     6361 aaagccggta aagttattca actgaataac ggttaatcaa atcccgactt atttcaataa
     6421 atcaatgaac gggtttgcga agatgaatag taatgaaata cctacagcga tcattgcgat
     6481 agcatctaaa agaccagcaa cgataaacat tttagtttgt aagcttgatg ctaattccgg
     6541 ctgacgtgca gatgactcta agaatttgcc acctaagata gcaaagccga tagcagtacc
     6601 aagagcagcg aaagctaata aaattgaagc accaataatt gttgctgtga ttactgattc
     6661 cataatgttc tccattagat tgaggaatta gcccgaaggc tgggttgttg ataaaaatac
     6721 atatcaagcg gtcaatattt tgcaaaaatt tgcgaaaaac ctaccgcttg taaggctagt
     6781 ttaatgatcc gctttgttat aagcgatact caaataaaca atcgttagca tcataaaaat
     6841 aaaggcttga agcgtaataa ctaaaatatg gaaaatcgcc caaaccaagt gtaatggaat
     6901 ccccaacgcc tgtagagcga agttgtctgc catatacatt actgcaataa ggataaagat
     6961 taactcacct gcatacatat taccgaataa acggaacgcc aatgaaatcg gttttgccaa
     7021 aagcgttacc gtttcaagaa taaagtttac cggaatgaat gcccagtggt taaacgggtg
     7081 taacgtgtat tctttcacta aaccgccaaa acctttcgat ttcaccgtgt aaaataaaat
     7141 taacgcaaat acacaaatag acatacccag tgttgcacta atatctgccg ttggtactgc
     7201 acgcaaatag tgaataccaa acagattggc taactgtggt aagaaatcga ccgggataag
     7261 gtcgatcgca ttcataataa acacccaaca gaaaatcgtt aatgctaatg gtgcaattac
     7321 attgcgaggt ccgtggaagt tatccttcac taaaccgttt acccactcaa taacaatctc
     7381 aacaaaacat tgcaattttc ccggaacgcc cgttgttgca ttttttgcta cacgcgagaa
     7441 cacccataaa aaaatcgcac ctgccactaa agagaagaac agtgtatcta aatgcactgc
     7501 ccaaaagcct tcacctgtcg ataaaaaact caagtgatgg ctaatatatt ctgctgtcgt
     7561 tccagccata acatatcctt ttaaattaaa attagtggtg atgtttgctt aatacaaacg
     7621 ggatcatatt atttaaaaat aatgcgataa aataacccac gaaaaacaac acaacatcta
     7681 gagcagggat aagtttaaaa cagccgatca ccaaaataat agtaaccaac catttcattc
     7741 cttcaccacg atagaaagcg gtcatttttg actgattttt tgcagatttc ttaaagaaaa
     7801 cccaatatac aaacacaaga tgaggcaaaa aactcgctaa agcaccgcct agaaaggaaa
     7861 aattaaaatt cattttccat aaagacactc caagtgcaaa aacgcacaag agaaaaaatt
     7921 caacctttaa tgcttgttta tattgtgatt ttgctctatt gattacagct gacacatttt
     7981 atcctttttg aataagtaaa ccacacaatc aggctaataa ttagccactg ttgaatcaaa
     8041 gaaaatccgt gctaaattat actttacaaa tgaattgttg caaccgaaaa gcgacacaaa
     8101 aacttgttat tcttcacatt ttt
//
LOCUS       DANLRK010000116         8037 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.116, whole genome shotgun sequence.
ACCESSION   DANLRK010000116 DANLRK010000000
VERSION     DANLRK010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8037
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.116"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            <1..1019
                     /gene="rnd"
                     /locus_tag="PXA33_001856"
     CDS             <1..1019
                     /gene="rnd"
                     /locus_tag="PXA33_001856"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HDL5121165.1"
                     /translation="FIRTRTYYPILGLIQLFDGQQVSLIDPNTISDFSPFVSLLADKN
                     IIKVLHACSEDLEVFEYQFKQLPEPMLDTQVMAAFAGIGISMGFAKLVSHYLNIELDK
                     GASRTDWLARPLSAEQLQYAAADVWYLLPVYEKLAQALTKSTWQSAVAEECVALLKKA
                     KQPINKEKAYKNIANAWRLNREELAILQVLAKWRIEEAQKRNLALNFVVKEASLVQIA
                     KSCPKHTSSLLEFMHPNEVRIHGKKILWLVEQGKAIESNDYPELIQRLVDEKGYKYYM
                     QAMLQKLLEIQPLDIVPELVASKRQLNQLFKWYIDGKPSEKIPELLIGWRKPFGEQLL
                     SVLS"
     gene            1076..1558
                     /gene="lrp"
                     /locus_tag="PXA33_001857"
     CDS             1076..1558
                     /gene="lrp"
                     /locus_tag="PXA33_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005695827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HDL5121166.1"
                     /translation="MENKKPPKALDAIDLKILNELQRNGKISNIELSKRVGLSPTPCL
                     ERVKRLEKNNVIMGYKALLNPELLDAPLLVIVEITLVRGKPDVFEEFNKAIQQLDEIQ
                     ECHLVSGDFDYLLKTRVADMVSYRKLLGTTLLRLPGVNDTRTYVVMEEVKQTNYLLLK
                     "
     gene            1569..4229
                     /locus_tag="PXA33_001858"
     CDS             1569..4229
                     /locus_tag="PXA33_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HDL5121167.1"
                     /translation="MIEQLKPHLKGKENLIKLMLLLTGFLGVYLLIAWASYSPLDNAW
                     SVASSITQTTLNKTGHFGAWSIDMLYAMFGKVAVLVPIALIIFSIYSLGMGLSVDMKW
                     KTVFIRLASFLLLMVGLAGLFSVLFSNSAYYLSGGFVGGVWETILSDTIGQFGALLIA
                     MLFTIAGLYFCSVQSLLPILTQFYEWIMAKSEESKTAESLQHLQNTEQKQPLVTENEA
                     VEVVDDHKPQFTDISAFKRPNISGLRHSENVEIDNREMYEIERDLELPTINILIQPDM
                     VNQVAEQATSGYELPNEESNVLQMPTIRLNTAIENEVDTIENSSSLEDLKIAESMLPQ
                     IQLDNDIEDTEEAIDDVREITPEFMVTPPKAMKDVSEQPAYPKGYGDTLIHPLLQRKV
                     ITEKPTTPLPTLDLLDKAPIQSQQITEQEIRETSVRLEAELANFGVKATVEDVLVGPV
                     VTRYEIQPAAGVKASKITNLASDIARGLMFKAIRITEVIPNKPYMGIETPNKHRETVW
                     LRDVLDSDEFRNTTATLPMALGKDISGEPVVVDMAKMPHLLVAGQTGGGKSVGVNTMI
                     LSLLFKLTPEQVRFIMIDPKVVELSIYNDIPHLLTPVVTDMKKAANALRWAVEEMERR
                     YLLVSHLQVRNIEGYNAKIDQAAAMNLPIPDPTWRPGDSMDSLPPPLQKLSYIVLIVD
                     EFADLMMSAGKEVEEYIMRIAQKARAVGIHLILATQRPSTDVITGVIKANIPSRIAFT
                     VASQIDSRTILDSGGAEALLGRGDMLYSGAGSPDIIRIHGAFMKDEEVQRVADNWRAR
                     GKPNYLESIVESRSEEADGKNDGGTGDLDPLFDEVVEYITETGSVSISNIQRRFSLGF
                     NRAARIVDQMEAQGIVSEPLKGGKREVLAR"
     gene            4381..5220
                     /locus_tag="PXA33_001859"
     CDS             4381..5220
                     /locus_tag="PXA33_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HDL5121168.1"
                     /translation="MRLDDIKESKNVEDRRFNNVGRTKVGRGKGGILTFIIVLVGAYY
                     GVDLTGLMGGGVEYEEQTSALNESEEKQLRTLSAKVLTTTENIWTSYFQQNGLTYKEP
                     SLVLYRGAIQTACGTGQSAMGPFYCPVDQKVYLDLSFYDDMRKKLRASGDFAFSYVIA
                     HEVGHHVQNLLGITSKTQRAQMKASSKAEANQISVNVELQADCFAGVWGHQLAKANRL
                     EEGDVEEAFNAAQAVGDDRLQQQSRGYVVPDSFTHGSSAQRLAWFRKGLTTGNPAVCN
                     TFN"
     gene            5309..6391
                     /gene="aroG"
                     /locus_tag="PXA33_001860"
     CDS             5309..6391
                     /gene="aroG"
                     /locus_tag="PXA33_001860"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HDL5121169.1"
                     /translation="MSYIKNDDVRITSIEELLPPIALLEAYPASDVAAETVAKTRQSI
                     HKILHGADDRLLVVIGPCSIHDPKAALEYAQKIKAMRADPKINQNLEVVMRVYFEKPR
                     TTVGWKGLINDPYLNETFALNDGLRIARKVLSDINDLSVPTAGEFLDMITPQYVADFM
                     SWGAIGARTTESQVHRELASGLSCAVGFKNGTNGSVKIALDAISSAKSPHHFLSVTKF
                     GHSAIVSTAGNPDCHIILRGGDSATNYDADSVAAACAAIEKSGGRPHVMIDFSHANSQ
                     KQFKRQMDVCADVCNQIAGGSDFISGVMIESHLVEGRQDLIEGKGLEALVYGQSITDG
                     CIGWEDSEKALFALAEAVEKRRNFAK"
     gene            6478..7170
                     /gene="tsaB"
                     /locus_tag="PXA33_001861"
     CDS             6478..7170
                     /gene="tsaB"
                     /locus_tag="PXA33_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HDL5121170.1"
                     /translation="MKPSILALDTATEACSVALQYNGNITFLDEISPRSHTQRILPMV
                     DELLNQANIELKEVNYLVFGRGPGSFTGVRVGVSVAQGLAMGANLPVVAVSNLKAMAE
                     EAYQKLGATKVIALIDARMNEVYFAQFERKGEEWNEVVAEQVCSPEVAISQFQMSEET
                     VVVGTGWAAYSQFSEQNLPLVISDITLPSSRYMLSIAETEIQKGNIQSAFEIEPVYLR
                     NEVTWEKLPHKQ"
     gene            complement(7187..7996)
                     /locus_tag="PXA33_001862"
     CDS             complement(7187..7996)
                     /locus_tag="PXA33_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDL5121171.1"
                     /translation="MTMTVIIILIICGIIVNITSALFGIGGGVLMMPILHSLFPELPL
                     QVISACSLTIVIGSATINLCLFHHQQIKINRKNVLIWSLAMMLGVQIGFELSFLFSDN
                     SIIAIFILTLLTLSFKTLFHKPRVKAIVPPKPKEAVSNATMCSLGGLIAGITGIGGGS
                     IMAPIVTQIRSVSPKQVAVYTNYMMVIGGIGNLYGYLSRPFVLNSTLATWQIGYVNFA
                     IVGTVVISSLFTVYFSMKLRNILSPELTTKLLGVILFTIACYMGFLQYLFT"
BASE COUNT         2529 a         1466 c         1796 g         2246 t
ORIGIN      
        1 agtttatccg tacacgtacc tattatccga tattgggttt aattcaactc tttgatggcc
       61 aacaggttag ccttattgat ccaaatacga tttctgactt ctcgcctttt gtgtcattat
      121 tagccgataa aaatatcata aaagtattac atgcctgtag tgaggattta gaagtttttg
      181 agtatcaatt taagcaattg ccagagccga tgttggatac tcaggtgatg gcagcctttg
      241 ccggcattgg tatttcgatg gggtttgcaa aattggtttc ccactactta aatattgaac
      301 tagacaaagg cgcttcccga accgattggc ttgccagacc gctaagcgct gaacaattac
      361 aatatgcggc agctgatgtt tggtatttat tgccagttta tgagaaattg gcacaagcat
      421 taacaaaaag cacttggcaa agtgctgtag cagaggagtg tgtcgctttg ttgaagaaag
      481 cgaaacagcc tatcaataag gaaaaagcct ataaaaatat tgccaatgct tggcgattga
      541 atcgggaaga gttagccata ttacaagtat tagcgaaatg gcggattgaa gaagcacaaa
      601 agcgcaatct tgccttaaat tttgtcgtga aggaggcaag tttagttcaa atcgcaaaaa
      661 gctgtcctaa acacacttct tcgctgttag aatttatgca cccaaatgaa gtcagaattc
      721 acggaaaaaa aatactatgg ttggttgagc aaggaaaagc aatagaatcg aatgattatc
      781 ctgaactcat tcagcgcttg gtggatgaaa agggttataa atattatatg caagcgatgt
      841 tgcaaaaatt attagaaatt caaccgcttg atatcgtacc ggagttggtt gcaagtaaac
      901 gtcaattaaa tcagctattt aaatggtata ttgatggaaa accatcagaa aaaattcctg
      961 aattattaat cggttggcga aaaccttttg gtgaacagct gctatcggtt ttatcttaaa
     1021 tttcattcgg gttacataga ctatgtacag tataattgcc taaaaaggag tttatatgga
     1081 aaataaaaaa ccaccaaaag cattagatgc gattgattta aaaatcttaa acgagctaca
     1141 acgtaacggg aaaatttcta atattgaatt atcaaagcga gttgggcttt ctccaacgcc
     1201 ctgcttagaa cgggtaaaac ggctagaaaa aaataacgta attatgggct ataaagcttt
     1261 acttaaccct gaattacttg atgccccttt actggtgatt gttgaaatta ctttggtgcg
     1321 tggtaagcct gatgtatttg aagaatttaa taaagcgatt caacaacttg atgaaattca
     1381 agaatgccac ctggtatcgg gggattttga ttatctgtta aaaacccgtg tggcggatat
     1441 ggtatcgtac cgtaagttat taggcacaac attgcttcgt ttgcccggcg taaatgacac
     1501 tcgaacctat gtggtgatgg aagaagtaaa acaaaccaac tatttgttgt taaagtaagg
     1561 agaattgggt gattgaacaa cttaaaccac acctaaaagg caaagagaac ttgatcaaac
     1621 ttatgctttt gttgaccggc tttttgggcg tttatttgct aattgcttgg gcaagttata
     1681 gtccattaga taatgcttgg tctgttgcaa gcagcattac acaaacaacc ttgaataaaa
     1741 caggtcactt tggtgcttgg agtattgata tgctctatgc aatgtttggt aaggttgcag
     1801 ttttagtacc tattgcatta attattttct caatttatag cttgggaatg ggtttaagtg
     1861 tcgatatgaa atggaaaact gtcttcattc gcctagccag tttcttatta ttgatggtcg
     1921 gtcttgccgg gttatttagc gtgctttttt caaactcagc ttattatctg tcgggtggct
     1981 ttgtcggtgg agtttgggag acaatattat ctgatactat cggacaattt ggtgccttat
     2041 tgattgcaat gctttttact atagctggct tatatttttg ctctgttcaa tcattattgc
     2101 caatattaac gcaattttat gagtggataa tggcaaaatc agaagagagc aaaacagctg
     2161 aatctcttca gcatttgcaa aatactgagc aaaaacaacc gcttgtaact gaaaatgaag
     2221 cggtggaagt tgtagatgac cataaaccgc aatttaccga tatctccgcc tttaaacgcc
     2281 caaacattag tggtttaaga cactcggaaa atgtagagat agataaccga gaaatgtatg
     2341 aaattgaaag agatcttgag ctaccgacta ttaatatttt aatacagcct gatatggtta
     2401 accaagttgc ggaacaagct acatcgggct atgagttacc aaacgaagaa tccaatgtat
     2461 tgcaaatgcc gaccattcgc ttaaataccg ctatcgaaaa cgaggtggat acaatcgaaa
     2521 acagttcatc attggaagac ctgaaaatag cagaatctat gttacctcaa attcagcttg
     2581 ataatgatat tgaagatact gaagaagcta ttgacgatgt gcgtgaaatt acaccggaat
     2641 ttatggtaac gccgcccaag gcgatgaaag atgtgtctga gcagccagcc tatccaaaag
     2701 gctatggtga tacgttaatt cacccgttgt tacaacgaaa agtgataacc gaaaaaccga
     2761 caacaccatt gccaacgcta gatttgttgg ataaagcacc gattcaaagc cagcaaatta
     2821 ccgaacagga aatacgagaa acctctgtgc ggctagaagc agaattagca aactttggcg
     2881 taaaagctac cgttgaagat gtattggtcg gtccggtagt gacccgttat gaaatccaac
     2941 cggctgccgg ggtaaaagcc tccaaaatta ccaatttagc gagcgatata gctcgtggct
     3001 taatgtttaa agcgattagg attactgaag ttatcccaaa caaaccttat atgggtattg
     3061 aaactccgaa taaacaccgt gaaacagtgt ggttgcgtga tgtgctagac agcgatgaat
     3121 tccgcaatac cactgcaaca ttgccaatgg cactaggtaa agacatcagt ggtgagcctg
     3181 tggtggtcga tatggcgaaa atgccacact tactggtggc agggcagacc ggtgggggta
     3241 aatcggttgg ggtgaatacg atgattttaa gtctattgtt caaacttaca ccagaacaag
     3301 tccgctttat tatgattgac cctaaagtgg tggagctttc catttataac gatattcctc
     3361 acttattaac gccggtagta accgatatga aaaaagccgc caacgcacta cgctgggcgg
     3421 tggaggaaat ggaacgccgt tatttgctgg taagccattt acaggtgcgt aatattgaag
     3481 gctataatgc gaaaatcgac caagcggcag cgatgaattt gccgattcca gatccaacgt
     3541 ggcgaccggg cgattcaatg gatagccttc cgcctccgct acaaaaatta agctatattg
     3601 tgctgattgt cgatgagttt gcggatttaa tgatgtctgc cggtaaagaa gtggaagagt
     3661 acattatgcg aattgctcaa aaagcccgag cggtgggaat tcacttaatt cttgctaccc
     3721 aacgtccgtc aaccgatgtg attaccggcg tgattaaagc caatattcca agccggattg
     3781 cgtttacagt agcgagccaa attgactcaa gaactatttt agattcaggt ggagcagagg
     3841 cattattagg gcgaggcgat atgctctatt caggagctgg cagcccggat attattcgga
     3901 tacacggtgc gtttatgaaa gacgaagaag tgcaacgcgt ggcggataat tggcgggcaa
     3961 gaggaaaacc aaactacttg gaaagcattg tggaatcccg ctcggaagag gctgatggca
     4021 aaaacgatgg cggaacgggc gatttagatc cgctatttga cgaggttgtt gaatatatca
     4081 cggaaaccgg ttcggtctct atcagtaata ttcaacgccg cttctcgctt ggttttaacc
     4141 gtgcggctag aattgtggat caaatggaag ctcaaggcat tgtttctgag ccacttaaag
     4201 gtggcaaacg agaagtgctg gcgaggtaaa acacatacaa gcggttagat ttctgtgaaa
     4261 ttttgcaaat tttgacaaaa atctaaccga ttgtatttat ctaatgctaa ataagcatag
     4321 tataaggcta gtctttgggc taaaattaac gcaattttta catcttttaa ggttgaatat
     4381 atgcgattag atgatattaa agaaagtaaa aatgttgaag acagaagatt caacaatgtg
     4441 ggtagaacta aggttggtag agggaaaggt ggaatattaa cctttattat cgtgcttgtt
     4501 ggagcttatt atggtgttga cttaacaggt ttaatgggcg gtggcgtaga atatgaggag
     4561 cagacatcag cattaaatga aagtgaagaa aaacagctaa gaaccttatc cgctaaagta
     4621 ttaacgacca cagaaaatat ttggacttcc tacttccagc aaaatggtct aacttataaa
     4681 gagccgtctt tggtgcttta ccgtggggca attcaaactg cctgtggcac cggtcaatct
     4741 gcaatgggtc ctttctattg ccctgtcgat caaaaggttt atttagattt atcattctat
     4801 gatgatatgc gtaaaaaatt aagagcatcg ggcgattttg ccttctctta cgtgattgca
     4861 cacgaggttg gccaccacgt ccaaaattta ttgggcataa caagcaaaac ccaacgggct
     4921 caaatgaaag cctcatctaa agccgaagca aaccaaattt cagtcaatgt agaattgcaa
     4981 gctgactgtt ttgccggcgt ttggggacac caactcgcaa aagccaaccg attagaggaa
     5041 ggcgatgtgg aagaagcctt taatgctgcc caagcagttg gagatgaccg attacagcaa
     5101 caaagtcgtg gctatgtcgt gccggatagc ttcacccacg gctcatctgc acagcgtttg
     5161 gcttggttta gaaaaggttt aaccacagga aatcctgcag tttgtaatac atttaattaa
     5221 aaaataatta aattatttga aatcttttta ttatcgtact actatgatag tacgatttta
     5281 ttttcatttt atttagagag tgaacacaat gtcttatatc aaaaatgacg atgtcagaat
     5341 taccagtatt gaagaattac taccacctat tgcattactg gaagcctatc cggcaagtga
     5401 tgttgcagcg gaaaccgttg ctaaaacccg tcaatcaatc cacaaaattt tacacggtgc
     5461 ggatgatcgc ttattagtgg tgattggacc ttgctccatt cacgacccga aagcggcatt
     5521 agaatatgca caaaaaatta aagcaatgcg tgctgatccg aaaattaacc aaaacttaga
     5581 agtggtgatg cgtgtctatt ttgaaaaacc ccgtacaacc gttggttgga aaggcttaat
     5641 taacgaccct tatttgaacg aaacctttgc cttaaacgat ggtttacgca ttgcccgtaa
     5701 agtattatct gatattaatg atttaagcgt gccgactgcc ggcgagtttt tggatatgat
     5761 tacaccacaa tatgtggctg actttatgag ctggggagca attggggcga gaacgactga
     5821 atctcaagtc caccgtgaat tagcctctgg tttatcctgt gcggtaggtt ttaaaaacgg
     5881 cacgaatggt agtgtgaaaa ttgcattaga tgcaatttct tccgcaaaat cgccacacca
     5941 tttcttatcg gtcactaaat tcgggcattc tgcgattgtt tcaacagcag gaaatcctga
     6001 ctgtcacatt attttgcgtg gtggcgatag cgcaaccaac tacgatgctg actccgttgc
     6061 agcggcttgt gctgcgattg agaaatccgg cggacgtcca cacgtgatga tcgatttcag
     6121 ccacgctaac agccagaagc agttcaaacg ccaaatggac gtttgtgctg atgtgtgcaa
     6181 ccaaattgca ggtggatcag actttatttc aggtgtgatg attgaaagcc acttggtaga
     6241 aggtcgccaa gacttaattg aaggcaaagg tttagaggcg ttagtttacg gacaaagtat
     6301 tacagacggc tgcattggct gggaagatag tgaaaaagcg ttattcgcac ttgctgaagc
     6361 ggttgaaaag cgcagaaatt ttgcaaaata atcttttctt gaaccgaggg cgaatataat
     6421 tcgcccttta tttgtttaac cttaaagcaa atttaattta ttcaaactaa acatatgatg
     6481 aaacccagca ttcttgcttt agatactgca acagaggctt gctctgtagc gttacagtat
     6541 aacggaaata tcacgttctt agatgaaatc agccctcgtt cccatactca acgtattttg
     6601 ccgatggtag atgaattact taatcaagcc aatatcgaac tcaaagaagt aaattattta
     6661 gtattcggac gtgggccggg cagttttacc ggtgtgaggg tcggtgtaag tgtggcacaa
     6721 gggttggcaa tgggagcgaa tttgccggtg gtagctgttt ctaacttaaa agcaatggca
     6781 gaagaagcat accaaaaatt aggtgcaaca aaggtaattg ccttaattga tgcccgaatg
     6841 aatgaagttt actttgctca atttgaacga aagggtgaag aatggaatga agtggtggct
     6901 gaacaagtct gttcgccaga agtcgcaata agtcaattcc aaatgagtga agagactgtg
     6961 gtggttggca caggttgggc ggcatattcg caattttctg agcaaaatct accgcttgtt
     7021 atcagtgata ttaccttgcc ttccagccga tatatgcttt cgattgctga aactgagatt
     7081 caaaaaggca atatccaatc tgcttttgaa atagaaccgg tgtatttgcg taatgaagtt
     7141 acttgggaaa aactacctca taagcaataa tctattgaac ttatggttaa gtgaataaat
     7201 attgcaaaaa gcccatataa caggcaatag tgaataggat tacaccaagt aatttagtag
     7261 ttaattcggg actcaagata ttgcgtaatt tcattgaaaa atagacagta aagagagaac
     7321 ttatcactac ggtgccgaca attgcaaaat tcacatagcc gatttgccaa gttgctaagg
     7381 ttgaattcaa cacaaaaggg cgggataagt agccatataa attgccgatt ccgccaatca
     7441 ccatcatata gttagtataa actgcaacct gtttcggtga aaccgagcgt atttgcgtga
     7501 caatcggagc cataatcgaa ccgccgccaa tgccggtaat acctgcaatt aaaccgccta
     7561 aactgcacat tgtggcatta gatactgctt cttttggttt aggtggtact attgctttaa
     7621 ctcttggttt gtgaaatagg gttttaaatg aaagtgtcag cagggttaga ataaagatag
     7681 cgataatgct gttatcagag aaaagaaaac tgagttcaaa cccaatttgt acgcctaaca
     7741 tcatcgcaag tgaccaaatc aacacatttt ttcgattgat ttttatctgt tgatggtgga
     7801 ataagcataa attgatagtg gctgaaccaa tcacaatggt aagagagcag gcggaaatca
     7861 cttggagagg cagttcagga aacagtgaat gtaaaatcgg catcattaaa acacccccgc
     7921 caatcccaaa tagtgcagaa gtgatattga ctataatgcc acaaatgatc aaaataatta
     7981 tcacggtcat tgtcataatc catctcctaa agtgtgataa gtatgcaaaa ttttcta
//
LOCUS       DANLRK010000117         7969 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.117, whole genome shotgun sequence.
ACCESSION   DANLRK010000117 DANLRK010000000
VERSION     DANLRK010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7969
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.117"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            56..190
                     /locus_tag="PXA33_001863"
     CDS             56..190
                     /locus_tag="PXA33_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/trimethylamine N-oxide reductase
                     NapE/TorE"
                     /protein_id="HDL5121172.1"
                     /translation="MRSELLKTLLLSGLILPLLMAFAVGAYGFIVWMLQLFVIGLPGA
                     "
     gene            194..1357
                     /gene="torC"
                     /locus_tag="PXA33_001864"
     CDS             194..1357
                     /gene="torC"
                     /locus_tag="PXA33_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HDL5121173.1"
                     /translation="MLGSIKALFTRPATKIGLGVLVGIGFIAGAISWHQFNKAMDHTS
                     TEEFCVSCHSMQQPLEELKKTAHWSNKSGVTATCSSCHLPHDYTEKYARKVQASREAW
                     AELSGKYKEEGSFEKHRLEMAEREWARFAANGSKECKACHSYERMNFDKMGEAARKAM
                     KPAAEKDQSCMDCHKGIAHHLPKADENALKSKFDQFVNKSPNQGKSYYTKGNAELFLD
                     EGLSQAIGQLETAVPVAFVQAGKNADLVELTMWRKEKGFGRIWYHDFGKNITDAVLTK
                     EFMANQPKFEVLETKEDPITGLNWQKVKLQAWIGKAQLLDDVTPIWANAENQYKTQCS
                     TCHRQPDVAHFDSNSWIGLFNGMVGFTNMDKQTGKEVLRYLQMHASDSEEAKH"
     gene            1457..3922
                     /gene="torA"
                     /locus_tag="PXA33_001865"
     CDS             1457..3922
                     /gene="torA"
                     /locus_tag="PXA33_001865"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HDL5121174.1"
                     /translation="MQQSRRQFLKNMSVMAAAVTMPNFLVPRNVFASEDLSQWKVSGS
                     HWGAMRAKIENGRVAEIKPFEFDKHPTEMLNGIKGLIYSDSRIRYPMVRLDWLKNRHN
                     SNTAQRGDNRFVRVTWDEALDLFYQELERVQKEYGPWALHTANVGWRSTGQFHSCGNH
                     MIRAIAMHGNSVGTAGDYSTGAGQTILPYVLGSTEVYSQGTSWEIILKESENIIFWAS
                     DPVKNLQVGWNCETHEAYAYLEKLKEKVAAKGVNVICVDPVKSKTQNFLGCEQQYINP
                     QTDVPFMLALAHTLYTENLYDKKFLDMYTVGFEKFLPYLLGETEDKVAKTPEWAAEIC
                     GISADKIREFARMLAGKRTQFIFGWAIQRQQHGEQPYWMGAVLASMLGQIGLEGGGIS
                     YAHHYSSIGIPESGAAMPGAFPLNIDEGQTPKYDNKDYKGYSDTIPVARMTDSLLHAG
                     ETIDYNGKKVTYAPFKMAIFTGCNQWHRHSERNKMKQAFQKLETIVSINYSWTATCRF
                     SDIVLPACTPFERNDIDAYGSYSNRGVIAMHKLVDPLYDSRSDFEIFRDLCRRFGKEK
                     EYSRGMDEMQWVEKLYKDCRRENRGKFEMPEFAEFWQKGYVLFPEGKPWVRHADFRED
                     PELHALGTPSGFIEIYSNKIASFGYDDCKGHPMWFEKAERSHGGKNSDKFPYWLQSVH
                     PDKRLHSQLCESKELRETYSVQGREPLYINPQDAAKHGIQDGDLVRVFNDRGQAIVGA
                     VLSDNFPTGVVRLQEGAWYSPLDESVGAIDTYGDPNTMTLDIGTSKLAQAVSANTCLV
                     NIEKFIGNAPAPNGFNGAIEVTA"
     gene            3922..4512
                     /gene="torD"
                     /locus_tag="PXA33_001866"
     CDS             3922..4512
                     /gene="torD"
                     /locus_tag="PXA33_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HDL5121175.1"
                     /translation="MTLAISREERQFIYGWFNAMLARELSDEQLNALQAGEFDDFFAY
                     LSELGFAEQVAKFQAEVTACRQLEFPRLELAADFAQLFLLEGNLSAIPYASAYLSGEA
                     LAKNLAEMDRLLGEFGLQVNRETKEPSDHLCVYLTLFDRLLAEKPLETQQQFLQNQLM
                     SWLPAWFAKAKTVKTNTGVYPALLRLFEQVLLKEQR"
     gene            4652..5341
                     /locus_tag="PXA33_001867"
     CDS             4652..5341
                     /locus_tag="PXA33_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDL5121176.1"
                     /translation="MQNNKTTYLNWYDLCVLTLILFGEAIYTSTQQYFITTEMQGNFE
                     ENLTFSNEQNYWALLTQLALLLIAMGYLKWRNFDFSRWVIRPSWLAFFSGIGLFILLS
                     LAMDLYFETLYGVYNLVYPEPFWAVLREVNISTVLYAILNGFYEEIFFLGICLAVKPE
                     YLKWAVIYSLIIRVSFHTYQGMESALGLGIWLGLIIYLLYSRSKQKNLFPFWVAHSIA
                     DILGLSLLAYF"
     gene            5499..6359
                     /locus_tag="PXA33_001868"
     CDS             5499..6359
                     /locus_tag="PXA33_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015586989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HDL5121177.1"
                     /translation="MKSIKKLTLATLILVGLTACGSGGSGSSSNNAKSKPQVELKKTE
                     EDKKTEEDKKTEEDKKTEEDKKTEEDKKTEEDKKTEEDKKAEEAKKIAEAKGIGNKEY
                     KDGLNELEEKNETGKNGEEIRTHGYLYNSHYSVVTAKMKQTLQENGRQMEPTVEVKGL
                     KTENLPTEGKATYKGEAFDSHGNNSNSVVGGELIYNVDFSSRTGSGLVKNVQGGSIEL
                     AQGEIKNDSIIASAHQKYNDQAVGNGSYNIQFFGPNAEEIGGKIELNGEGEGSMKQML
                     GIAGTREEQK"
     gene            6408..7802
                     /locus_tag="PXA33_001869"
     CDS             6408..7802
                     /locus_tag="PXA33_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HDL5121178.1"
                     /translation="MYKKHFLFLLVSPLVMAETVEFQQDPKEFDGQRQAVKFEQEIAK
                     KDLKLTAYLEEDNSLKGIEQHINQAILRQDWAKLERLLGDYAKQEQFEPLLLDYALAA
                     LAYAKKDYRSAAFYYQQLLKKQPDLTYPRFDLALILKEDYRYREAEQEFKQLKTDDLR
                     NAVNSALAQIEEEESWKPDFYLQYTQTNNVNNASSSPTVNVNGRIFIKESDSLPQSAK
                     GVRYGVGLSKMRNIGGNHFVGVDLGYNGVYYWNNHDYSEQSVSLTPKYSYRTATYRIG
                     VSPFIEQNWLGSSRYNYQFGSSINAVKLFERGLISGSFSHLQKRYSEQHIADRYNGYR
                     NQLTLGTQWQAVKNWRFFANLNASREITREKAQSSNKWLATIGVIYQTPSWASSVSVG
                     YGKRYFADKHYLFGYKRQDKEYQANVAVWNRSWHWQGIVPKLNFRYQKIDSNIADFYS
                     RSNKEVFMTLEKLF"
BASE COUNT         2446 a         1593 c         1879 g         2051 t
ORIGIN      
        1 ttgtttctga agataaaata attaacactt attagttaat aaaaaaggag ttgaaatgcg
       61 ttcagaacta ttaaaaacgc ttttgctatc agggctgatt ttaccgctat tgatggcgtt
      121 tgccgtagga gcttacggct ttattgtgtg gatgttacaa ctgtttgtta ttggtttacc
      181 gggagcgtag aaaatgttag gttcaatcaa agcattattt actcggcctg caaccaaaat
      241 cgggttggga gtgttagttg gtatcggttt tattgccgga gcaattagct ggcatcaatt
      301 taataaagcg atggatcata ccagcactga agagttttgc gtgagttgcc actcaatgca
      361 gcagccgtta gaggagctta aaaaaaccgc ccactggtcg aataaaagcg gggtaacggc
      421 aacctgttca agctgccact taccgcacga ttataccgaa aaatatgccc gcaaagtgca
      481 ggcaagccgt gaggcgtggg cagaattatc cggcaaatat aaagaagaag gctcatttga
      541 aaaacaccgc ttagaaatgg cagagcgtga gtgggcaaga tttgccgcca atggttcaaa
      601 agagtgtaag gcttgccaca gctatgagcg aatgaatttt gataaaatgg gcgaagcagc
      661 tcgtaaggca atgaaaccgg ctgcggagaa agatcaaagt tgtatggatt gccacaaagg
      721 cattgctcac cacttaccga aagccgatga aaatgcactg aaatcgaagt ttgatcagtt
      781 tgtgaataaa tccccaaacc aaggcaaatc ttactacacc aaagggaatg ccgaattatt
      841 tctagacgaa ggtttaagcc aagctatcgg gcaattagaa accgccgtgc cggtggcatt
      901 tgtgcaagcg ggcaaaaatg cggatttggt tgagctgaca atgtggcgta aagagaaagg
      961 tttcggtcgc atttggtatc acgatttcgg gaaaaacatt accgatgcgg ttctaaccaa
     1021 agagtttatg gcaaatcagc cgaaatttga ggtgttggaa accaaagaag atccgattac
     1081 cggcttgaat tggcaaaaag tgaaactaca agcgtggatt ggcaaagctc aattattaga
     1141 cgatgttacc ccaatctggg cgaatgcaga aaaccaatac aaaacccaat gtagcacttg
     1201 ccacagacag cctgatgtcg cccacttcga ttccaacagc tggatcggct tattcaacgg
     1261 aatggtcggc tttaccaata tggataaaca gaccggcaaa gaggtgctac gttatttaca
     1321 aatgcacgcc tccgatagtg aagaagcgaa gcattaataa tattactccc ctctttacta
     1381 aagaggggaa ttaatcgagc gttgaataac attctctgta ctagctcacg tttttacaaa
     1441 taaagaagga caagatatgc aacaatctcg ccgtcaattt ttaaaaaata tgtctgtgat
     1501 ggctgccgct gtcacaatgc cgaatttcct cgtgcctcgc aatgtgtttg cgagtgaaga
     1561 cctcagtcaa tggaaagttt ccggctctca ctggggggca atgcgtgcca aaattgagaa
     1621 cggacgagtg gcagaaatca aaccgttcga gtttgataaa cacccgaccg aaatgctcaa
     1681 cggcattaaa ggcttaattt acagcgattc ccgtatccgc tacccgatgg tgcgtttaga
     1741 ttggctcaaa aaccgccata acagcaacac cgcacagcgt ggcgataacc gctttgtgcg
     1801 ggtaacgtgg gacgaagccc tcgacctgtt ctaccaagag ttagaacggg tacaaaaaga
     1861 gtacggcccg tgggcgttac acaccgccaa tgtgggctgg cgttcaaccg gtcaattcca
     1921 cagttgcggt aaccatatga tccgagcgat tgcgatgcac ggtaacagtg tcggcacggc
     1981 aggcgactat tctaccggtg cagggcagac cattctgccg tatgtgctgg gttcaaccga
     2041 agtctattcg cagggcacgt cttgggaaat catcttaaaa gagagcgaaa acatcatttt
     2101 ctgggcaagc gacccggtga agaacctgca agtgggttgg aactgcgaaa cccacgaagc
     2161 ctatgcctac ttggaaaaac tgaaagagaa agtggcagca aaaggcgtga atgtgatctg
     2221 tgttgatccg gtgaaaagca aaacccagaa tttcttaggt tgcgaacagc agtacatcaa
     2281 tccacaaacc gatgtgccgt ttatgttggc acttgcccac acgctctaca ccgagaacct
     2341 gtacgacaaa aaattcttgg atatgtacac ggtcggcttt gaaaaattcc tgccgtatct
     2401 gttaggcgaa accgaagaca aagtggcgaa aaccccggaa tgggcggctg aaatttgtgg
     2461 tatttccgcc gacaaaatcc gtgaatttgc ccgaatgctt gccggcaaac gcacccaatt
     2521 tattttcggc tgggcaattc aacgccaaca acacggcgaa caaccgtact ggatgggagc
     2581 ggtgctagcc tcaatgttag ggcaaatcgg cttggaaggc ggcggcatca gctatgctca
     2641 ccattacagc tctatcggta ttcctgaatc cggtgcggca atgccggggg cgttcccgct
     2701 caatattgat gaggggcaaa ccccgaaata cgacaacaaa gattacaaag gctacagcga
     2761 caccattccg gtggctcgta tgaccgacag cttgctccac gccggcgaaa ccattgatta
     2821 caacggcaaa aaagtgactt acgctccgtt caaaatggcg attttcaccg gctgtaacca
     2881 atggcaccgc cattccgagc ggaacaaaat gaaacaggcg ttccaaaaac tggaaaccat
     2941 tgtttcgatc aactacagtt ggacggcaac ctgccgcttc tccgacatcg tgctgccggc
     3001 ctgtacccca tttgaacgca acgatattga cgcctacggc tcatacagca accgtggcgt
     3061 gattgcaatg cacaagctgg ttgatccact ctacgactct cgctccgact ttgagatttt
     3121 ccgagatctc tgccgccgtt tcggtaaaga gaaagagtac agccgtggaa tggacgaaat
     3181 gcagtgggta gaaaaactct acaaagattg tcgccgtgaa aacagaggta aatttgaaat
     3241 gccggagttt gctgaatttt ggcaaaaagg ctatgtgctg ttcccagagg gcaaaccttg
     3301 ggttcgccac gccgatttcc gtgaagatcc ggaattgcac gcacttggca caccgtccgg
     3361 ctttatcgaa atttacagca acaaaatcgc ctctttcggc tatgacgatt gcaaaggtca
     3421 cccaatgtgg tttgagaaag ccgaacgttc acacggcggt aaaaattccg acaaattccc
     3481 gtactggttg caatcagttc acccggacaa acgcttacac tctcaacttt gtgaatcgaa
     3541 agaactgcgt gaaacttaca gcgtgcaagg gcgtgaaccg ctttacatta acccacaaga
     3601 tgccgccaaa cacggcattc aagacggaga tttagtgcga gtgtttaacg accgtgggca
     3661 agcgattgtg ggggcggtgc tttccgacaa cttcccgacc ggcgtagtgc gtttacaaga
     3721 aggggcttgg tattcacccc ttgacgaaag cgtgggggcg attgacacct atggcgaccc
     3781 gaacacaatg accctcgaca tcggcacgtc caagttggca caagcggttt ccgccaacac
     3841 ttgcttggtg aacatcgaga aatttatcgg caatgccccg gctccgaatg gctttaacgg
     3901 tgcgattgag gtaacggctt aatgacgttg gcaatcagtc gtgaagaacg gcaatttatc
     3961 tatggctggt ttaacgcaat gctcgcccga gaactttcgg acgagcagtt aaatgcctta
     4021 caagcgggcg aatttgacga tttttttgca tacctcagcg aattgggttt tgccgaacag
     4081 gttgcaaaat ttcaagcaga agtgaccgct tgtaggcagc tggaatttcc tcgtctagaa
     4141 ctggcggctg actttgccca actgttttta ttggaaggca atctcagtgc cattccttac
     4201 gcctccgcct atttaagcgg cgaagcgtta gcgaaaaatt tggcggaaat ggacagactg
     4261 ctgggcgaat ttgggctgca agttaatcga gaaaccaaag agccgagcga tcacctttgc
     4321 gtttatctca ccctttttga ccgcttgctt gctgaaaaac cgttggaaac gcaacagcaa
     4381 tttctgcaaa accaactgat gagctggctc cctgcgtggt tcgccaaagc caaaaccgtc
     4441 aaaactaaca ccggcgttta tccggcgttg ctaaggttgt ttgaacaggt gttgctgaag
     4501 gaacaaagat aaagttattt atttgagaat gatttgtatt gagcgaattg agaaaggaag
     4561 ttaaaatttc aacttccttt tttattggct ggatttatcc acattattac aagcggttat
     4621 ttttataaaa aaatttacaa aggagacatt aatgcaaaat aataaaacca cctacctaaa
     4681 ttggtacgat ttatgcgtgc taaccctgat cctgtttggt gaggcaattt atacctcaac
     4741 ccagcaatat ttcatcacca ccgaaatgca aggtaatttt gaagaaaatt taaccttttc
     4801 aaatgaacaa aattattggg cattactgac gcaacttgca ttgttgctga ttgcaatggg
     4861 ctatctaaaa tggcggaatt ttgatttcag ccgttgggtg attcgtccct cttggctggc
     4921 tttttttagc ggtatcgggt tgtttatctt gctctcgtta gcgatggatc tctattttga
     4981 aacattatat ggtgtttaca acctagtgta tccggagcca ttttgggcag tattgagaga
     5041 agtgaatatt tctaccgtgc tgtatgcgat tttaaacggc ttttatgaag aaatcttttt
     5101 cctcggtatt tgcttggcgg ttaaacctga atatttaaaa tgggcggtga tttattcact
     5161 gattattcgg gtgagttttc atacctatca aggaatggaa agtgcgttag gattaggaat
     5221 ttggctcggt ttaattattt atttgctcta ctcacgctca aagcagaaaa acctatttcc
     5281 attttgggtg gcacacagta ttgcggatat tttggggctg agtttgttgg cttattttta
     5341 gttaaaggtg ctacatttaa gcggcataag ttgatagagc tgatcgtacc agtagcataa
     5401 aaacattatt atttgtatgg aaaggtgctt ttaggttaca atataaccga tagttattgg
     5461 ttataactaa aaattatttt taatttaacg gagcaaatat gaagagtatc aaaaaattaa
     5521 ccctagccac tttgatttta gtgggtttaa ctgcttgtgg tagcggtggt tcaggtagca
     5581 gttctaataa tgctaaatcg aaaccacaag ttgagttaaa aaaaactgaa gaggataaaa
     5641 aaactgaaga ggataaaaaa actgaagagg ataaaaaaac tgaagaggat aaaaaaactg
     5701 aagaggataa aaaaactgaa gaggataaaa aaactgaaga ggataaaaag gctgaagaag
     5761 caaaaaaaat tgcagaggca aaaggaattg gcaataaaga atataaagat ggattaaatg
     5821 agttagaaga aaaaaatgaa acaggtaaaa acggagaaga gattcgaacc catggttatc
     5881 tctataacag ccattattcc gttgtaactg ccaaaatgaa acaaacttta caggaaaatg
     5941 gtcgtcaaat ggaacctacg gttgaagtga aagggcttaa aactgagaat ttaccgacag
     6001 agggcaaagc aacatataaa ggagaagcct ttgattctca cggtaataac agcaattcag
     6061 tagtaggtgg agaattaatc tataatgttg atttctctag tcgtaccgga tcaggtctag
     6121 ttaaaaatgt acagggcggt tctatcgagt tagcacaagg cgaaataaaa aatgatagta
     6181 ttatagcttc tgctcaccaa aagtataatg atcaggctgt tggaaatggt agttacaata
     6241 tccaattctt tggtccaaat gcagaagaaa ttggagggaa aattgagctt aatggtgagg
     6301 gcgaaggctc aatgaagcag atgttaggta ttgcaggaac ccgagaagaa caaaaataac
     6361 atttaatacc caatgccgtt taatcttaaa cggcattctt ttttactatg tataaaaaac
     6421 attttctatt tttacttgtt tcgcctttag taatggcgga aacggttgag tttcagcaag
     6481 atcccaaaga gtttgatggg cagcgacaag cggttaaatt tgagcaagaa attgcaaaaa
     6541 aggatttaaa attaaccgct tacttggagg aagataattc tcttaaaggt attgaacagc
     6601 acattaacca agcgattctt agacaagatt gggcgaagct ggaaaggctg cttggcgatt
     6661 atgcaaagca agagcaattt gaaccgctac ttttagacta tgctttggcg gcattggctt
     6721 atgctaaaaa agattatcgc tctgcggcgt tttattatca gcagttgctg aaaaaacagc
     6781 ctgatttaac ctaccctcgt tttgatttgg ctctgatttt aaaagaggat tatcgctaca
     6841 gggaagcaga gcaagaattt aagcaactca aaaccgatga tttacgcaat gcggtgaaca
     6901 gtgcgttggc tcaaatagag gaggaagaga gctggaagcc ggatttttat ttgcaatata
     6961 ctcaaaccaa caatgtgaat aatgcttctt cgtcgccaac ggtaaatgta aacgggcgga
     7021 tttttattaa agaatcagac agcttgccgc agtcggcaaa aggcgtaaga tatggcgttg
     7081 gcttgagtaa aatgcgaaat atcggcggta atcattttgt gggagtcgat cttggctata
     7141 acggcgttta ttactggaat aaccacgatt atagcgaaca gtcggtctca ctaacaccaa
     7201 aatatagcta cagaacggca acttatcgta tcggggtttc gccctttatt gagcagaatt
     7261 ggctgggtag ctcacggtat aactatcaat ttggtagcag tatcaatgct gttaaacttt
     7321 ttgaacgagg gctgatttcc ggctcattca gccatctgca aaaacgttat agtgaacagc
     7381 atattgccga ccgttacaat ggttatcgca accaactcac gctaggtacg caatggcaag
     7441 cggtcaaaaa ttggcgattt tttgcaaatc ttaacgctag ccgtgaaatt acccgtgaga
     7501 aagctcaatc ttccaataag tggctggcga ccattggcgt aatttaccaa accccaagct
     7561 gggcaagttc ggtaagtgtc ggatatggca agcgttattt tgctgataaa cattatctgt
     7621 ttggctataa acgccaagat aaggaatatc aagcgaatgt ggcggtgtgg aatcgaagtt
     7681 ggcactggca agggattgtg ccgaaattga atttccgtta tcaaaaaatc gatagcaata
     7741 ttgccgattt ctactctcgt agcaataaag aagtctttat gaccttagag aaactatttt
     7801 aaggctgtgt atgaatgtcg gggagaaaaa ttttctcccc gatatttatg tgtagaagat
     7861 cagacttgat ctgacaattc actctaaaaa gtgagagttt cccgtttaga atatgagtgt
     7921 caaaaatcaa tctaaacaaa aaggaaactc tcatgtttta ttctaacaa
//
LOCUS       DANLRK010000118         7773 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.118, whole genome shotgun sequence.
ACCESSION   DANLRK010000118 DANLRK010000000
VERSION     DANLRK010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7773
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.118"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            49..1476
                     /gene="hldE"
                     /locus_tag="PXA33_001870"
     CDS             49..1476
                     /gene="hldE"
                     /locus_tag="PXA33_001870"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HDL5121179.1"
                     /translation="MMMQYSPKFNNAKVLVLGDVMLDRYWFGATNRISPEAPVPVVKV
                     QDIEERAGGAANVAMNIASLGVPVTLHGLIGDDDAGRALDKLLNSHQIQNHCVVINSH
                     PTITKLRILSRHQQLLRLDFEEGFHNVDSTALLAKLSQEITAYGALILSDYGKGTLES
                     VQQMIQIARNAKVPVLIDPKGTDFERYRGATLLTPNMSEFEAVVGHCKDEDEIVSKGL
                     KLIADYDLSALLVTRSEKGMTLLRPNHEPYHLPTQAKEVYDVTGAGDTVISVLATAIA
                     DGRAYEEACYLANAAAGVVVGKLGTSTVSPVELENAIHQRTATGFGVVSEAELKAIVE
                     EAKKRGEKIVMTNGCFDILHPGHVSYLENARKLGDRLIVAVNTDASVKRLKGEARPIN
                     DLAARMAVLAGLASVDWVVPFDEDTPQRLISEILPDLLVKGGDYKPEEIAGSQEVWAN
                     GGDVRVLNFENGCSTTNVIKKIQAL"
     gene            1490..2044
                     /gene="pyrR"
                     /locus_tag="PXA33_001871"
     CDS             1490..2044
                     /gene="pyrR"
                     /locus_tag="PXA33_001871"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HDL5121180.1"
                     /translation="MAREKIIIDTEQFQRTISRISHQIIEKHGDLQDVVIVGIKRRGA
                     EIAELIQKRIEDLAQTRLPYMALDITFYRDDLDLIYQDPVFMGADNQLNLNGKKVILV
                     DDVLFTGRTIRAALDALLDFGRAARIELVIFVDRGHRELPIRADYVGKNIPTAKNEQI
                     QVQTLSYDGINQVVLVPAANKESA"
     gene            2117..3067
                     /locus_tag="PXA33_001872"
     CDS             2117..3067
                     /locus_tag="PXA33_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007523149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL5121181.1"
                     /translation="MKMKLVKSLFAVAVATMSLTQTAHAFEERVVALVNDTPIMQSQV
                     QRVLGKKKATETAQRAAIDQVIDDMLVQQAMKEAGVKVSPAAVNQAVENVAIQNGITY
                     GQLLDALDYQGITLEQFKRNIAQQMAMEQVRHISISKSIQVAPQQVQALAKELMAKDK
                     AAGKLKTVSGKEHRISHILLKTNPILNDAQAKAKLSSLTTDINSGKISFEEAAKTHSV
                     DYASGADGGDLGFNFLEIYDPAFANVASKIKPNQISAPFKSQFGWHILKVTDTRNGDR
                     TEDAYHQRAYQQLVNKQAEEASKDWVKALRKTANIQYVGQ"
     gene            3129..3995
                     /gene="rsmA"
                     /locus_tag="PXA33_001873"
     CDS             3129..3995
                     /gene="rsmA"
                     /locus_tag="PXA33_001873"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HDL5121182.1"
                     /translation="MSSTNSKKHLGHTARKRFGQNFLHDMNIIHSIVSAINPKNGQFL
                     LEIGPGLGALTEPVAELVDKLTVVEIDRDLAERLRHHPFLNQKLTIIEQDALRFNFRD
                     YFNQLNLDEDSVRVFGNLPYNISTPLIFHLLTFHDLIQDMHFMLQKEVVKRLCAAPNS
                     KAYGRLTIMAQYYCQVIPVLEVPPTAFKPAPKVDSAVVRLVPYNQLPYPAKDVYWLNR
                     VTTQAFNQRRKTLRNALSTLFSAEQLDALGVDLTARAENLTIADYVRLANWLHDNPPA
                     IDTTEELVDEDC"
     gene            complement(4106..4735)
                     /gene="upp"
                     /locus_tag="PXA33_001874"
     CDS             complement(4106..4735)
                     /gene="upp"
                     /locus_tag="PXA33_001874"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HDL5121183.1"
                     /translation="MKIVEVKHPLVKHKLGLMREAEISTKDFRELANEIGSLLTYEAT
                     KDLEMESVEINGWSGEKIAVERIKGKKVTVVPILRAGLGMMDGVLEHIPSARISVVGI
                     YRDEETLKPVPYFSKLANDVDERLAIITDPMLATGGSMVATIDLLKKAGCKQIKVLVL
                     VAAPEGIKVLEAAHPDVELYTASIDSHLNENGYIIPGLGDAGDKIFGTK"
     gene            complement(4760..6445)
                     /gene="fadD"
                     /locus_tag="PXA33_001875"
     CDS             complement(4760..6445)
                     /gene="fadD"
                     /locus_tag="PXA33_001875"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HDL5121184.1"
                     /translation="MEKIWLNNYPPYSPKTIETDQYESLVEMFEAAIQRHPDIPAYIN
                     MGQVLTFRKLEERSRSFAAYLQNELRLKKGDRIALMMPNLLQYPIALFGALRAGLVVV
                     NVNPLYTPRELEHQLNDSGAKAIVIVSNFAATLEKIVFNTEIKHVILTRMGDQLSFGK
                     RTLVNFVVKYIKKLVPKYKLPHAVSFREALSIGKQRQYVKPNIVSQDLAFLQYTGGTT
                     GVAKGAMLTHQNLIANMMQAKWIVEPLLGNSANMIGLVPLPLYHVLALSMNCLLFIEL
                     GLTGLLITNPRDIPGFIKELKKYKVSVITGVNTLFNALLNHESFKEVDFSHLKLSVGG
                     GAAIQSSVAKRWHDLTGVHIIEGYGMTECSPLISATRGDSVEYSGSIGVPVPNTDIRI
                     VDDEGKEVPLGERGELVVKGPQVMQGYWNRPDETAQVLQDGWMATGDIVVMGDDLNLR
                     IVDRKKDMIIVSGFNVYPTEIEEVISQNPKVNEVVAIGVPSEASGESIKIFVTKKDES
                     LTRDELRTYCRQFLTGYKIPRDIEFRDELPKSNVGKILRRVLRDEELAKVNKA"
     gene            complement(6423..6887)
                     /locus_tag="PXA33_001876"
     CDS             complement(6423..6887)
                     /locus_tag="PXA33_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HDL5121185.1"
                     /translation="MMIEQATVVEYQNGVAIVQCYAKSSCGGCVAQSACGSKALSALA
                     GEKIDPRFRIEVDEPLKVGDKVELGLAENILLKSVFLIYGIPLLVLVMTAVGFSQFFA
                     NELIVLFIMLLSTGITFWAVRKLIHKHSKQANFSPIFLGKVLGDYNGKNMAK"
     gene            complement(6884..>7773)
                     /locus_tag="PXA33_001877"
     CDS             complement(6884..>7773)
                     /locus_tag="PXA33_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MucB/RseB C-terminal domain-containing protein"
                     /protein_id="HDL5121186.1"
                     /translation="AYLTAMSEAHKKSTYELVYILQQGSDTESFRLRHAFTHNKEYAQ
                     LLNLDHSREEIILKNNKVSYLGYNFRPFSLNSSQILDNLPNVLYTDYANLKGYAFLDA
                     GKDRISDRITKVIRMKPNDTLRYAYILWIDEETHLLLKSQVLNMDNLVLEEFRVLQLY
                     QSPELDRIANVIESLMLPTLTTVKKEEFPKSDGWQVKWLPTGFRLIQQQTVTGATYQV
                     ESEYVDSRVYSDGLSTLTIYIMPSQGVSFNEYAWQQGKLTILNQTINDRDIVIIGDVP
                     LQSAKQIMNNIGFKEGAQP"
BASE COUNT         2290 a         1686 c         1537 g         2260 t
ORIGIN      
        1 ggcacaagcg tggacgcttg cgccatcatt taacagagaa tattaactat gatgatgcaa
       61 tactctccaa aatttaataa tgctaaagtg ctggtgcttg gcgatgtaat gttagaccgt
      121 tactggttcg gggcaaccaa tcgaatttcg cctgaagccc ctgtgccggt ggtgaaagta
      181 caagatattg aagagcgtgc cggcggtgcg gcaaacgtgg cgatgaacat cgccagcctt
      241 ggcgtaccgg taacattaca cggcttaatt ggcgatgatg atgccgggcg tgctttagat
      301 aaattactta attcccacca gatccaaaat cattgtgtgg tgattaattc gcacccaacc
      361 atcacaaaat tgcggattct ttcccgccat cagcaattat tgcggttgga ttttgaagag
      421 ggttttcaca atgtagattc taccgcccta cttgcaaaac tttcgcaaga aattaccgct
      481 tacggagcat taattctctc ggattacggc aaaggcacat tggaatcggt gcaacaaatg
      541 atccaaattg cccgcaatgc taaggtgccg gtgctgatcg acccgaaagg cacagacttt
      601 gaacgctatc gtggggcaac attgcttaca ccaaatatgt cggaatttga agcggtggtt
      661 gggcattgta aagatgagga tgagattgtc agcaaagggt taaaactgat tgccgactat
      721 gatttatctg cactattagt tacccgttcg gaaaaaggaa tgaccttact tcgcccgaac
      781 cacgagcctt atcatctgcc gacccaagcc aaagaggttt atgatgtaac cggtgcaggc
      841 gataccgtaa tcagtgtatt agccactgcg attgccgatg gcagagccta tgaagaagcc
      901 tgctatttag ccaatgctgc cgccggtgta gtcgtaggta aattagggac ttcaaccgtc
      961 agcccggttg agctggaaaa tgcgattcac caacgcactg ccaccggttt tggcgtggtt
     1021 tctgaagccg aattaaaagc gattgtggaa gaagcgaaaa aacgtggcga aaaaatcgtg
     1081 atgacgaacg gttgctttga tattcttcac cccggtcacg tttcctattt ggaaaacgcc
     1141 cgtaaattag gcgaccgatt aattgtagcc gtcaataccg atgcttcggt aaaacgccta
     1201 aaaggcgaag ctcgcccgat taatgattta gctgcccgta tggcagtttt agctggttta
     1261 gcctctgtag attgggttgt gccttttgat gaagatacac cacaacgttt aatcagtgaa
     1321 atcctacctg atttattggt aaaaggtggt gattacaagc cggaagagat tgccggcagc
     1381 caagaagtat gggcaaacgg cggcgatgtg cgagtattga actttgaaaa cggctgttct
     1441 accactaatg tgatcaagaa aattcaagca ctataatggt aataagcgaa tggcgagaga
     1501 aaaaatcatt attgataccg agcaatttca gcgcacaata tctcgtattt cacatcaaat
     1561 tattgaaaaa cacggtgatt tgcaagatgt agtgattgtc ggcatcaaac gtcgaggagc
     1621 tgaaattgcc gagttaatcc aaaaacgcat tgaagattta gctcaaacac gcttgcctta
     1681 tatggcgtta gatattacgt tctaccgtga tgacctagac ttaatttatc aagatcccgt
     1741 atttatggga gccgataatc agctaaatct gaacggtaaa aaagtgattt tggttgatga
     1801 tgttctgttt accggcagaa ccattcgggc ggcacttgat gcactcttag attttggcag
     1861 agctgccaga attgaactgg tgatttttgt cgatagaggt catcgggaat tgcctatccg
     1921 agccgattat gtcggaaaaa atattccaac ggctaaaaac gagcaaattc aggtgcaaac
     1981 gctctcgtat gatgggatta accaagtggt cttagttcct gctgccaata aagaaagtgc
     2041 ttaaattaag tgaacctttt cgctaaactt atctctaagg aaaagttact gcttaattac
     2101 ctccattagg attgttataa aaatgaaatt agtcaaatca ttatttgctg ttgccgttgc
     2161 aacaatgagt cttacccaaa ccgcccacgc atttgaagaa agagtggtgg cgttagtgaa
     2221 tgatacccct attatgcaaa gccaggttca acgtgtgctt ggcaagaaaa aggcgacaga
     2281 aactgctcaa cgtgcggcta ttgatcaagt gattgatgat atgttggtgc aacaggcaat
     2341 gaaagaagcc ggcgttaaag tcagcccagc tgcggtaaac caagcggttg aaaatgtagc
     2401 aattcaaaac ggcattactt acggtcagtt attagatgcg ttagattatc aaggtattac
     2461 acttgaacaa ttcaaacgta atattgctca acaaatggca atggaacaag ttcgccatat
     2521 cagcattagc aaatcgattc aggttgcacc acagcaagtg caagctctgg cgaaagagtt
     2581 aatggcgaaa gataaagcag caggcaaatt gaaaaccgtt tctggcaaag agcatcgcat
     2641 tagtcatatt ttgctcaaaa ccaacccgat tttaaatgat gctcaagcca aagcaaaact
     2701 tagcagctta actactgata ttaactcagg caaaatcagc tttgaagaag cggcaaaaac
     2761 ccattctgtt gattatgcct caggggcaga cggtggagat ttaggtttta acttcttaga
     2821 aatttacgat ccggcatttg cgaatgttgc atcaaaaatc aaaccaaacc aaatttctgc
     2881 tccgtttaaa tcgcaatttg gttggcatat tttaaaagtc accgacactc gtaacggaga
     2941 cagaaccgaa gatgcttacc accaacgtgc ttatcagcaa ctggtgaaca agcaagccga
     3001 agaagcctct aaagattggg taaaagcgct tagaaaaacc gccaatattc aatatgtcgg
     3061 tcaataatta aaatttttaa gaacctcgca taatgcgggg ttcttttctt atttcaaaaa
     3121 ggataacaat gagttctaca aattccaaaa aacatttagg tcataccgct cgtaagcgtt
     3181 tcgggcaaaa ctttttgcac gatatgaaca ttattcacag catagtttca gccattaacc
     3241 cgaaaaacgg acaattttta ttagaaatcg gtccgggttt aggagcatta accgagccgg
     3301 ttgccgaatt ggtagataaa ctcacggtgg ttgaaattga ccgtgattta gcagagcgtt
     3361 tacgccacca cccgttttta aaccaaaaac tcaccattat tgagcaagat gccctacgct
     3421 ttaatttccg tgactatttt aaccaactca acttagacga agactcggtg cgtgtgtttg
     3481 gtaacttacc ttacaacatt tctacgccct tgatattcca cctgctgacc tttcacgact
     3541 taattcaaga tatgcacttt atgttgcaaa aagaggtggt gaaacgttta tgtgccgcgc
     3601 caaacagtaa agcatacggg cgtttaacca ttatggctca atactattgc caagtaattc
     3661 cggtgctgga agtgccgcca accgccttta aaccggcacc aaaagtcgat tctgcggtag
     3721 tgcgtttagt gccttataac caactgcctt atccggcaaa agatgtgtat tggctcaatc
     3781 gggtcacgac tcaagccttt aaccaacgcc gtaaaacctt acgcaatgct ctttcaaccc
     3841 tgtttagtgc ggagcaactg gacgccttag gcgttgattt aaccgcccga gcggaaaacc
     3901 ttaccattgc cgattatgtg cgtttggcta actggctaca cgacaacccg ccggcaattg
     3961 atacaaccga agaattggtt gatgaagatt gctaatcaat aaattacaag cggtcatttt
     4021 tctaccaaga tttgcaaatc tttagtgaaa aatggccgct tgtttaaatc agctttactt
     4081 gttttgctgt tacttagttt tgatgttact tggtgccgaa aattttatcc cctgcatcgc
     4141 ctaagcctgg aatgatatag ccgttttcgt ttaagtgact atcgattgag gcggtgtaga
     4201 gttcaacatc tgggtgggcg gcttctaata ctttaatccc ctctggagcg gcaactaaca
     4261 ctagcacttt aatctgctta cagcctgctt ttttcaggag gtcaatggtt gctaccattg
     4321 agccaccggt tgcaagcatt gggtcggtga taatggctaa acgctcatca acatcgttgg
     4381 ctagtttgga gaaatacggc acgggcttta aggtttcttc gtcacgataa atcccaacca
     4441 cactaattct ggcacttgga atatgctcta acacaccgtc catcatacct aagccagcac
     4501 gcagaatcgg cacaacagtc acttttttgc ctttaattcg ctcaaccgca attttctctc
     4561 cgctccagcc attgatctcc acactttcca tttccagatc tttggttgct tcgtaagtaa
     4621 gcagactacc aatttcattc gctagctcac ggaaatcttt ggtgctgatt tccgcttcac
     4681 gcattaagcc caatttatgc tttacaagtg ggtgttttac ttcaacaatt ttcattttaa
     4741 gctccttaat tagtgtaatt taagccttat tcacctttgc taattcttcg tctcttaaca
     4801 ctcggcgaag aattttgccg acattacttt ttggtaattc gtcacggaat tcgatgtctc
     4861 ttggaatttt gtagccggtt aagaattgac gacaataggt tctcaattca tctcgagtta
     4921 aactttcatc ttttttggtt acaaaaattt taatgctttc gccggaagcc tcactcggca
     4981 caccaatcgc cactacttca ttcacttttg ggttttggga aatcacttct tcaatttcgg
     5041 ttgggtacac attaaaaccg gagacaataa tcatatcttt tttacgatca acaatgcgta
     5101 agttcagatc atcccccatt accacaatgt caccggttgc catccaacca tcttgtagca
     5161 cttgtgccgt ttcatcagga cggttccaat agccttgcat tacttgcggc cctttcacca
     5221 ccagctcgcc ccgttcgccg agtggtactt ctttgccttc atcatccaca atacgaatgt
     5281 cagtatttgg cactggcacg ccaatagagc cggaatattc caccgaatcg ccacgggttg
     5341 cactaatcag cggagaacat tctgtcattc cataaccttc gataatatgt acgccggtta
     5401 aatcgtgcca gcgttttgcc acgctagatt ggatcgctgc accgccacct acacttaatt
     5461 ttaggtgtga aaaatcaacc tctttaaaac tttcgtgatt aagtaatgca ttaaacaatg
     5521 tatttacccc agtaatcact gaaactttgt acttttttaa ttctttaata aagcctggaa
     5581 tatcgcgcgg attagtaatt aataatccgg taagtcctaa ttcgataaag agcaagcaat
     5641 tcatcgaaag tgctaagacg tgatacagcg gtaaaggtac caatccaatc atattggcag
     5701 aattacccaa caatggctca acaatccatt ttgcctgcat catattagca attaagtttt
     5761 gatgagtcag cattgcccct ttcgctacac ctgtagtacc gccagtgtat tgtaaaaagg
     5821 cgagatcttg gctgacaata ttcggtttta catattgacg ctgtttgcca atgcttaagg
     5881 cttcacggaa gctgaccgca tgtggcaatt tatatttcgg cactaatttt ttgatgtatt
     5941 tcaccacaaa attgactaag gtgcgtttgc cgaatgaaag ttggtcgccc attcgggtca
     6001 aaataacgtg cttaatctca gtattaaaga caattttttc taaggtagcc gcgaagttcg
     6061 agactatcac aatcgctttc gcaccactgt catttaattg atgttctaat tcacgcggcg
     6121 tataaagcgg gttgacattc accaccacaa gcccggcacg taatgcgcca aataaagcga
     6181 tcggatattg caataaattc ggcatcatca atgcgattcg atcacctttt ttcaggcgta
     6241 attcattttg caaataagcg gcaaacgagc ggctacgttc ttccaattta cggaacgtta
     6301 aaacctgccc catattaata taagctggaa tatcagggtg gcgttgtatc gctgcttcaa
     6361 acatttccac taaagattca tattgatctg tttcaatcgt ttttggtgaa taaggcggat
     6421 aattatttag ccatattttt tccattatag tctcctaaaa ccttacctaa aaagataggt
     6481 gaaaaatttg cttgtttgct gtgtttatga atcaattttc ttacagccca gaatgttata
     6541 cctgttgaaa gcaacattat aaataaaaca ataagctcat ttgcaaaaaa ttgcgaaaat
     6601 ccgaccgctg tcatcacaag aaccagcaga ggaatgccat aaattaaaaa cacgcttttg
     6661 agcaagatat tttcagctaa ccctagttca accttatcgc ctacttttag gggttcatct
     6721 acctcaatac gaaaacgagg gtcaattttt tcacctgcta atgctgataa tgctttgctg
     6781 ccacaagcag actgagctac acagccacca cagctacttt tagcataaca ttgtacaata
     6841 gccacaccat tctgatattc caccaccgta gcctgttcga tcatcatggc tgagctcctt
     6901 ccttaaaacc aatattgttc ataatttgct tagccgattg caacggcaca tcaccaataa
     6961 tcacaatatc tctatcatta atcgtttgat ttaaaatcgt taatttacct tgttgccaag
     7021 cgtactcatt aaaactcacg ccttggctag gcataatata aatagttaat gtagataaac
     7081 catcactata tactctgcta tctacatact cactttccac ttgatatgtc gcacctgtga
     7141 ctgtttgttg ctgaattaac ctaaaccctg tcggcaacca cttcacctgc caaccatcag
     7201 acttaggaaa ttcttctttt ttcactgtcg ttaatgttgg taacattagt gattctatta
     7261 catttgcaat cctatccaat tctggagatt gataaagttg caaaactctg aattcttcta
     7321 ataccaaatt atccatattc aagacttggc ttttaagtaa taaatgtgtt tcttcatcaa
     7381 tccatagtat ataggcatat cttaaagtat catttggctt catacgaatt acctttgtaa
     7441 ttcggtcaga aatacgatct ttaccagcat ctaaaaaggc ataacctttt aaatttgcat
     7501 aatctgtgta aagtacatta gggaggttat ctaaaatctg tgaactattt aaactaaaag
     7561 gacgaaaatt ataccccaaa tagctgacct tgttattttt taaaataatt tcttctcggc
     7621 tgtgatctaa attcagtaat tgggcatatt ctttattatg ggtaaaagca tgacgtaaac
     7681 ggaaagattc tgtatcactt ccctgttgaa gaatataaac taattcataa gtggattttt
     7741 tatgtgcttc tgacatagcc gttaaatatg cta
//
LOCUS       DANLRK010000119         7664 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.119, whole genome shotgun sequence.
ACCESSION   DANLRK010000119 DANLRK010000000
VERSION     DANLRK010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7664
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.119"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(27..1862)
                     /gene="glmS"
                     /locus_tag="PXA33_001878"
     CDS             complement(27..1862)
                     /gene="glmS"
                     /locus_tag="PXA33_001878"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HDL5121187.1"
                     /translation="MCGIVGAVAQRDIAEILVDGLHRLEYRGYDSAGVAVLNADDKTM
                     QIVRRVGKVKALDEALDTKPLLGGTGIAHTRWATHGEPSETNAHPHRSGKIAVVHNGI
                     IENYEELKIVLQERGYVFQSQTDTEVIAHLVEWELRSASSLLEAVQKTVVQLRGAYGT
                     VVLNEEEPEHLIVARSGSPLVIGYGVGENFLASDPLALLSVTRRFAYLEEGDVAEITR
                     RTVDIYDREGNKAEREIHEGNFEADAADKGQYRHYMQKEIFEQPVAIMNTLDGRIKDG
                     KVDISAIAPNAAEILSKVEHVQIVACGTSYNAGMVSRYWFESIAGVSCDVEIASEFRY
                     RKFVTRPNSLLITLSQSGETADTLAALRLAKESGYMAAMTVCNVASSSLVRESDFAFL
                     TKAGVEIGVASTKAFTTQLTCLLLLNVAIGRLQGRMSEEQEHHIVQSLQRLPAQIESA
                     LVFDKQIEKLSEDFADKHHTLFLGRGEYYPVAMESALKLKEISYIHAEAYAAGELKHG
                     PLALIDSDMPVVVVAPENDLLEKVKSNIEEVRARGGQLYVFADHDAGFEEAEGFKTIV
                     MPKVDEVTAPIFYTVPLQLLSYHIALIKGTDVDQPRNLAKAVTVE"
     gene            complement(1910..2671)
                     /locus_tag="PXA33_001879"
     CDS             complement(1910..2671)
                     /locus_tag="PXA33_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HDL5121188.1"
                     /translation="MSKRNTQQRRHLIVQMVQEKNEVSVDELAHFFETSEVTIRKDLT
                     ALEESGFLLRKYGGAVKIPSEMMEEAFIEQLSIQKKMIAQAARDCIRDHNRIIIDSGS
                     TTGALVKALNQTGLVVMTNSLNLATELTSLECEPTVLMTGGTWDSRSESFQGKVAEQV
                     LRSYDFDQLFIGADGLDLARGTTTFNELVGLSQVMAEVSREVIVLIESQKIGRKMPNI
                     ELKWQNITKIITDSQLSFETKEQIEKMGVEVVIAK"
     gene            complement(2828..3028)
                     /locus_tag="PXA33_001880"
     CDS             complement(2828..3028)
                     /locus_tag="PXA33_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HDL5121189.1"
                     /translation="MKSILKVVGAMVLMVAVSGCNNMTKTQRNTAIGAAIGGVAGHAI
                     GESTGATLGGAALGGVIGSQIK"
     gene            3253..4287
                     /locus_tag="PXA33_001881"
     CDS             3253..4287
                     /locus_tag="PXA33_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL5121190.1"
                     /translation="MKLKKLSLALSTLLLGSAFANSALAEGRLTVYCSATNELCEQEV
                     QAFGKKYNVKVAFVRNGSGSTLAKIEAEKNNPQADVWYGGTLDPHSQAAEMGLLEPYK
                     SPNLEQIIEKFRDPAKLKGNYSSAVYMGILGYGVNLDRIKKLGIEKVPSTWEDLLDPR
                     LAGEIQIADPQSSGTAYTAIATFVQLLGEEKAFDYFRKLHKNISQYTKSGITPARNTA
                     RGETAIGIGFLHDYAIEKKNGANIEMTAPTDGTGYELGGVSILKGARNLDNAKLFVDW
                     SLSKEAQELSWQKGNAFQVLTNTTAESSPYALDPKNLNLINYDFEKYGSSEERKRLID
                     KWVNEVKLAK"
     gene            4355..6391
                     /locus_tag="PXA33_001882"
     CDS             4355..6391
                     /locus_tag="PXA33_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016534092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDL5121191.1"
                     /translation="MQNFSFSRPLVWVLIGLLGFLLLPSKALDYGLFDSTSDELLAAM
                     GWASLNITWAWFLPLLVFLLPNSTACKTRAKLELVGLGVLFAFIFLSAIVLKISLGYA
                     VLPLIIAIMGLATPNLATLKVMQGDRFIIGSLLSIILLIFFFIVYPTIAIFVSMFYNG
                     SEFVPSQVLTILQQPYVVRVIGNSLSVAGTVGVLSTLFGLAFALYTTRIAKRSAFIGR
                     IFSILPIVTPPFVVGLGVTLMLGRSGYVTEFLVDYFGFANNWLYGFTGIVIAHTLALT
                     PMSFMILEGALKSIHPSVEEAAYTLRSNRYQAFFNIIFPLLKPALANSFLVVAIQSLA
                     DFSTPLVLGGSFDVIATQIYFYIAGSQLDYASASTLGAILLVFSLAIFVIQYLWIGNR
                     SYVTVSGKSYRGDVQDLPSGMKWLIICTLLFWGTFNLVLYGSIFYGSFTVNWGVDYTL
                     TLDNYIKLFGQGFSDGGFPSLIQTVIFAAAAAPITALFGLLIAYVTVRRDFKGKKTLE
                     FLTLLCFAVPGTVAGVSYILAFNDAPIYLTGTGMIIILSMVMRNMPVGMRAAIAGLGQ
                     LDKSLDEASLSLKAGSFKTMLFVVLPLLKPALLSALVTSFVRAMTTVSAIVFLVTADT
                     RVATSYILNRVEDGEYGIAIAYGSILIVVMMAIILIFDWIVGDTRIARSKAKKM"
     gene            6489..7538
                     /gene="fbpC"
                     /locus_tag="PXA33_001883"
     CDS             6489..7538
                     /gene="fbpC"
                     /locus_tag="PXA33_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric ABC transporter ATP-binding protein"
                     /protein_id="HDL5121192.1"
                     /translation="MNNDFLVLKNVTKSFGKVTVIDNLNLSIPQGKMVTLLGPSGCGK
                     TTVLRLVAGLENPTSGQIFIDGEDVTQSSIQHRDICIVFQSYALFPHMSIGDNVGYGL
                     KMQGVPKEERLQRVKEALELVDLGGFEDRFVDQISGGQQQRVALARALILKPKVLLFD
                     EPLSNLDANLRRSMREKIRELQQRLNITSLYVTHDQSEAFAVSDEVIVMNKGKIMQKA
                     PAKELYLRPNSLFLANFMGESSIFEGTIAGNQININGYQFTLANAEQFELPDGEYLVG
                     IRPEAIHLAEQGNISQLCKIKNAVYMGNHWEIIAEWEGKDLLVNTKPENFYPHIKQAY
                     VHFIEQGVFLLKKEL"
BASE COUNT         2087 a         1726 c         1561 g         2290 t
ORIGIN      
        1 caagatatct tggggtttaa ctaacattac tctacagtca ccgcttttgc aaggttacga
       61 ggttggtcaa cgtccgttcc tttgattaag gcaatgtgat atgaaagtag ttgtaacggc
      121 acggtgtaga aaatcggtgc ggttacttca tcaactttcg gcattacaat cgttttgaag
      181 ccttctgctt cttcaaaacc tgcatcgtga tcggcaaaca catataactg accgcctctt
      241 gcacgcactt cttcaatatt ggatttcact ttctcaagca agtcattttc cggtgctacc
      301 accactaccg gcatatcgct atcaattaat gctaacgggc cgtgttttaa ctcgcctgcg
      361 gcataagctt ctgcgtgaat atatgaaatc tcttttaatt tcaatgcaga ttccatcgca
      421 accgggtaat attctccacg acctaagaac aatgtgtggt gtttatccgc aaaatcttcc
      481 gataattttt cgatttgttt atcgaacact aacgcacttt caatttgtgc tggtaagcgt
      541 tgtaaagatt gcacgatatg gtgttcttgc tcttcactca ttctgccttg taagcgtcca
      601 atcgccacat ttaataacaa taagcaagtt aattgagtag tgaatgcttt ggttgatgct
      661 acaccaattt caacaccagc tttggttaag aatgcaaaat cggattcacg cactaatgaa
      721 gaacttgcca cattacacac cgtcattgcc gccatatagc cagattcttt cgctaaacgc
      781 agtgcagcta acgtatctgc ggtttcacct gattgtgaaa gcgtgattaa taagctgttc
      841 gggcgagtaa cgaatttacg gtaacggaat tcggaggcaa tttccacatc acagctcacg
      901 cctgcaatgg attcaaacca ataacgtgaa accataccag cattgtaaga ggttccacaa
      961 gccacaattt gcacgtgttc tactttcgat aaaatctctg ccgcattcgg tgcaatggct
     1021 gaaatatcta ctttaccgtc tttgatacga ccatctaagg tattcataat cgcaaccggc
     1081 tgctcaaaaa tctctttttg catatagtga cgatattgtc ctttatctgc cgcgtctgcc
     1141 tcaaaattgc cttcgtgaat ttcacgctct gccttgttgc cttcacggtc ataaatatcc
     1201 actgtgcgac gggtaatttc ggctacatca ccttcttcta gataagcaaa acggcgagtt
     1261 acgcttaata atgcaagtgg gtctgaagca aggaagttct cgcctacacc gtaaccaatc
     1321 actaacgggc tacctgagcg agccacaatt aaatgctccg gctcttcttc atttaatacc
     1381 actgtgccgt aagcaccacg cagttgcacc acggtttttt gtaccgcttc caataatgaa
     1441 gaagcagaac gcaattccca ttcaactaaa tgagcaatga cttcggtatc ggtttgagat
     1501 tggaacacat aaccacgctc ttgtaacact atttttaact cttcgtagtt ttcgataatc
     1561 ccgttatgaa ctactgcaat tttgccactg cggtgtgggt gagcattagt ttctgacggc
     1621 tcgccgtgcg ttgcccagcg agtgtgtgcg atcccagtgc cgcctaatag tggcttagtg
     1681 tctaacgcct catctaaggc tttgacttta ccgacacggc gtacaatttg cattgtttta
     1741 tcatctgcat ttaatactgc tacaccagca gagtcatatc cacggtattc taagcgatgt
     1801 aaaccatcaa ccaagatttc tgcgatatca cgttgtgcta ctgcacctac aattccacac
     1861 ataaatttgc tttcctatgt ttaagtttaa aaattcattt gagctactgc tattttgcaa
     1921 taaccacttc gacacccatt ttttcgattt gttctttcgt ttcaaaactt aattgactgt
     1981 cggtaataat tttggtaata ttttgccatt tcaattcaat attcggcatt ttccgaccga
     2041 ttttttgcga ttcaattaac acaatcactt cacggctcac ttctgccatc acctgactta
     2101 agccaactaa ttcattaaag gtagtcgtgc ctcgagccaa atctaaaccg tccgcaccaa
     2161 taaaaagctg atcgaaatca tatgaacgca atacctgttc tgctactttg ccttgaaagg
     2221 actcagaacg gctatcccaa gtgccgccgg tcatcaacac tgttggttcg cattcgaggg
     2281 aagtcaattc cgtcgctaaa ttcagcgaat ttgtcatcac caccaagccg gtttggttca
     2341 atgcttttac caaagctccg gtcgtgctgc cgctgtcaat aatgatgcga ttatgatcac
     2401 gaatacaatc acgtgcggct tgagcaatca ttttcttttg aatcgaaagt tgctcaataa
     2461 acgcttcttc catcatttcc gatggaattt ttaccgcccc accatactta cgcaataaaa
     2521 aaccactttc ttccaaagcg gttaaatctt ttctaatcgt aacttcagac gtctcaaaga
     2581 aatgagcgag ttcatctaca ctcacttcat ttttctcttg taccatttga acaattaaat
     2641 ggcggcgttg ttgcgtgtta cgttttgaca ttataatctt cacttaaatt tcgtttcaaa
     2701 acttttgcat tataaagaaa gctcgcgaaa agaaaagcag aaaatgcaaa atttacaaaa
     2761 tctatacaaa ataaaaccgc ttgtaatatt gatttacaag cggttgattt cttgaaattt
     2821 tttgcaatta tttgatttgg ctaccaatca cgccaccaag tgcggcacca cctaaagttg
     2881 caccggtaga ttcaccaatc gcgtgaccag ccacaccgcc aattgctgca ccgattgcag
     2941 tattacgttg cgtttttgtc atattgttac agccagacac agcaaccatt aataccattg
     3001 caccaacaac tttcaaaata gatttcataa taatccctca taattaatca atttcaaata
     3061 agccaatatt cttggctatg ctataagatt taatttattt agaaaggttc aactactacc
     3121 acctatgaat tgcaaatttt ttcacaattt aggccgcttg ttgattgaac tgtgagcttg
     3181 ctcacagaat tttatggtat ttcttgctat attgtatagc aatctatgcg ataactcatt
     3241 aaggaaataa aaatgaaatt aaaaaaatta tctttagcac tctcgacttt attactcggt
     3301 tcagcctttg ccaactctgc tttagcggaa ggtcgtttaa ccgtttattg tagtgccact
     3361 aatgaacttt gtgagcaaga agtgcaagca ttcggcaaaa aatataatgt taaagtagca
     3421 tttgtgcgta atggttcagg cagtacattg gcgaaaattg aagcggagaa aaacaatcct
     3481 caagctgatg tatggtatgg cggtacttta gatccacatt cacaagctgc agaaatgggt
     3541 ttactcgagc cgtataaatc accgaatctc gaacaaatta tcgaaaaatt ccgtgatccg
     3601 gcaaaattaa aaggcaacta ctcttcggct gtgtatatgg ggattttagg ttatggtgtg
     3661 aatttagacc gaatcaaaaa actcggcatt gaaaaagtgc ctagcacttg ggaagacttg
     3721 ctcgacccac gcctagccgg tgaaatccaa attgccgacc cacaaagctc cggcacggct
     3781 tatactgcga ttgcgacctt cgtacaactt ttaggagaag aaaaagcctt cgactatttc
     3841 cgcaaattac acaaaaacat ttcacaatat accaaatccg gcatcacacc ggcacgcaat
     3901 accgcccgtg gtgaaaccgc tatcggtatc ggtttcttac acgactatgc gattgagaag
     3961 aaaaacgggg ctaacattga aatgaccgca ccaacagacg gcacaggcta cgaattaggt
     4021 ggtgtgagta tcctcaaagg tgcaagaaat cttgataatg caaaactgtt cgtggattgg
     4081 tcgctttcta aagaagctca agaactctct tggcaaaaag gcaatgcgtt ccaagtatta
     4141 accaacacca ccgcagaatc ttccccttat gctctagatc cgaaaaactt aaacttaatc
     4201 aactatgatt tcgagaaata cggctcgagc gaagagcgta aacgcttgat tgataagtgg
     4261 gtaaatgaag taaaattagc gaaatagtgt taatctttcc actgagggga taagcaatta
     4321 tcccctctct aattataaga atctgtgagg tgttatgcag aatttttcct tttcccgacc
     4381 gcttgtttgg gtgcttatcg ggttactcgg ttttctcttg ttaccgtcta aagccttaga
     4441 ttatgggctg tttgactcaa cctctgatga actgctcgcc gcaatgggct gggcgagtct
     4501 gaacatcact tgggcgtggt ttttaccgct tttggtattt ttattgccaa attcgaccgc
     4561 ttgtaaaact agagccaagc tggaattagt agggctgggc gtgctgttcg cctttatttt
     4621 cctgtcagcg attgtgctga aaatcagcct cggttatgcg gtgttgccgt tgattattgc
     4681 cattatgggg cttgcgaccc caaatttagc aacgctcaaa gtaatgcagg gtgaccgctt
     4741 tattatcggc tcgttactga gcattattct gctgattttc ttttttattg tgtatccgac
     4801 catcgccatt tttgtgtcga tgttctacaa cggctcggaa tttgtgccaa gccaagtgct
     4861 gacaattttg caacaacctt atgtcgttcg agtcatcggt aactcgctct cggttgccgg
     4921 cacggtgggc gttctctcca ccctattcgg cttagccttt gctctctaca ccactcggat
     4981 tgctaaacgc tcggcgttta tcgggcggat tttctcgatt ttacctatcg tcaccccgcc
     5041 gtttgtggtc ggtttaggcg taaccctgat gcttgggcgt tccggctatg ttactgaatt
     5101 tttagtggat tactttggct ttgccaacaa ttggctgtac ggtttcaccg gtattgtgat
     5161 agcccatacc ctcgcgctca cgccaatgtc gtttatgatt ttggaagggg cattgaaatc
     5221 cattcaccca tcggtggaag aagcagctta caccctgcgt tccaaccgct atcaagcctt
     5281 ttttaacatt attttcccat tgttaaaacc ggcattggca aactcctttt tagtggtggc
     5341 aattcaatcc ctcgccgatt tcagtacgcc gttagtattg ggcggtagct ttgatgtgat
     5401 tgcaacccaa atctacttct acattgccgg ttctcaactg gattatgcct ctgctagcac
     5461 gcttggggca attttgttgg tgttctcctt agccattttc gtgatccaat acctctggat
     5521 cggcaatcgc tcttatgtga cggtgtccgg caaatcctat cggggtgatg tccaagactt
     5581 accaagcggt atgaaatggc tgattatttg caccttattg ttctggggaa ccttcaactt
     5641 agtgctgtac ggcagtattt tctacggcag tttcaccgtg aactggggtg tggattatac
     5701 cttaaccctt gataactaca tcaagctatt cgggcaaggg tttagcgatg gcggtttccc
     5761 ttcgttaatt caaaccgtga ttttcgctgc cgctgccgcc ccgattactg cactattcgg
     5821 cttgctgatc gcctatgtga cagttcggcg tgatttcaaa ggcaagaaaa ccctggaatt
     5881 tttaaccctg ctctgctttg ccgtaccggg aacggtggca ggtgtttctt acattctcgc
     5941 ctttaacgat gccccgattt acctaaccgg caccggtatg atcatcattc tctcaatggt
     6001 aatgcggaat atgcctgtgg gtatgcgagc ggcaattgcc ggcttagggc agttggataa
     6061 atcgctggac gaagcctcgc tctcgctgaa agccggttcg ttcaaaacga tgctgtttgt
     6121 ggtgttaccg ctactcaaac cggcactgct ctccgccctc gtcaccagct ttgtgcgggc
     6181 aatgacgact gtcagtgcga ttgtattctt agtcaccgcc gacactcgtg tggcaacctc
     6241 ctacattctc aaccgtgtgg aagacggtga atacggcatt gcgattgcct acggctcaat
     6301 tttaattgtg gtgatgatgg cgattatcct gatttttgac tggattgtcg gcgacacccg
     6361 tatcgcccgt tctaaggcga aaaaaatgta aaaagacatc tactctctcc ctttatggga
     6421 gagagacaga aaaatacttc gttcagaagt atttttcaga gagagggata taattaaaag
     6481 gacaatatat gaacaacgat ttcttagtat taaaaaacgt caccaaatct ttcggcaagg
     6541 tgacggtcat tgataatctc aacctcagta ttccgcaagg taaaatggtg acattactcg
     6601 ggccgtccgg ctgtggtaaa accaccgtac tgcgtttagt ggcagggctg gaaaacccga
     6661 cttcggggca aatttttatt gatggcgaag atgttaccca aagttcgatc caacaccgtg
     6721 atatttgcat tgtgttccaa tcttatgcgt tatttccgca tatgtcgatt ggcgataatg
     6781 tcggctacgg cttgaaaatg cagggcgtac cgaaagaaga acggctgcaa cgagtgaaag
     6841 aggcgttgga attggtggat ttaggcggct ttgaagatcg ctttgtcgat caaatctctg
     6901 gcggacaaca acaacgtgtc gccctcgccc gtgccttaat tctgaaaccg aaagtgctac
     6961 tgttcgatga accgctgagt aaccttgacg ctaacttacg ccgttcaatg cgggagaaaa
     7021 tccgtgaatt acagcaacgt ttaaacatta cttcccttta tgtgactcac gatcagtccg
     7081 aagcctttgc ggtttcagat gaagtaattg tgatgaacaa gggaaaaatt atgcaaaaag
     7141 ctccagccaa agagttatac cttcgcccta attctttatt cttagcaaat tttatgggtg
     7201 aaagcagcat ttttgaaggc acgatagctg gcaaccaaat taatataaat ggatatcaat
     7261 ttacgttagc aaatgctgaa caatttgagt tacccgatgg ggaatactta gttggcattc
     7321 gtccagaagc tattcattta gctgaacaag gaaatataag tcaactctgt aaaattaaaa
     7381 atgcagttta tatgggaaat cattgggaaa ttatagctga atgggaaggg aaagatttat
     7441 tagtcaatac taaacctgaa aatttttacc ctcatatcaa acaggcctat gtacatttta
     7501 tcgaacaagg tgtttttctt ttaaagaaag agctataacg aatttatact aaattaagag
     7561 gaaggtagtt ataactattt agcaattttg agaaaaatag gccgcatgca aaatctcttg
     7621 atgacaagcg gtcttttctc gccgattttt tgcaaatgaa atga
//
LOCUS       DANLRK010000120         7603 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.120, whole genome shotgun sequence.
ACCESSION   DANLRK010000120 DANLRK010000000
VERSION     DANLRK010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7603
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.120"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            291..2747
                     /gene="thrA"
                     /locus_tag="PXA33_001884"
     CDS             291..2747
                     /gene="thrA"
                     /locus_tag="PXA33_001884"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HDL5121193.1"
                     /translation="MRVLKFGGTSLANPERFMQAAEIIEKAHLADQAAGVLSAPAKIT
                     NHLVAIVDKAIAGESFQSNLSEATEIFHNIINGLYATNNHFAKEALLALVEAELNQIQ
                     QVAAEAAQTKSLSDKIAATIHCRGEKLSIAMMQAWFEAKGYEVTRIDPVEKLLAHGSY
                     LESSVDIAESTKRVDAGSIPKKNIVLMAGFTAGNEQGELVLLGRNGSDYSAACLAACL
                     KADVCEIWTDVDGVYTCDPRLVPDAVCLETMSYQEAMELSYFGAKVIHPRTIGPLVPL
                     NIPCLIKNTGNPDGKGTLIDGNVANDNLKVKGITNLDNVAMFNVSGSGMQGMVGMAAR
                     VFSTMSKAGISVILITQSSSEYSISFCVPAKSAEKALAALNTEFAQELSENLLDPIDI
                     IKDLSIVSVVGDGMRTAKGIAARFFSALAQANISIVAIAQGSSERSISAVVPLNKAIE
                     AVKATHKSLFNSKRVVDMFLVGVGGVGSELIEQVKQQKEFLAKKDIEIRVCALANSDK
                     MLLNENGLNLDNWKEDLEKAIQPSDFDVLLSFIKFHHVVNPVFVDCTSAQSVANLYAR
                     ALKEGFHVVTPNKKANTSGYDYYLEMRENARQSQHKFLYETNVGAGLPVIENLQNLLA
                     AGDEVEKFEGILSGSLSFIFGKLEEGLTLSQATLIAKEKGFTEPDPRDDLSGADVARK
                     LLILVRETGLPLEFEDIEIEGVLPKGFSEGMSRDEFLQALPSLDEEFSKRVEAAKAEG
                     KVLRYVGSITGDKCRVAIEAVDENHPLYKVKDGENALAFLTRYYNPIPLLLRGYGAGN
                     SVTAAGIFADILRTLQGGVN"
     gene            2747..3694
                     /gene="thrB"
                     /locus_tag="PXA33_001885"
     CDS             2747..3694
                     /gene="thrB"
                     /locus_tag="PXA33_001885"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HDL5121194.1"
                     /translation="MALRIYAPASSANISVGFDSLGAAISPIDGSLLGDVVQIEESEQ
                     QFELESAGYFVRKLPKEPQKNIVYQAYVLFSERLKLRNGSVKNLRLTLEKNMPIGSGL
                     GSSACSIVAALVALNKFHDEPFSKMELLEMMGELEGRISGSIHYDNVAPCYLGGLQLM
                     TQSLGNICQPLPFFDEWYWVLAYPGVEVSTAEARAILPKSYTRQDMIQQARYLGSFVH
                     ACHTHQDILAATMMKDLIAEPYREDLLPNFPAVRQGCKDLGALAVGISGSGPTMFAIA
                     PDLEHAQRLVSYLEKEYLQNNEGFIHICKVDNQGARELK"
     gene            complement(3809..5185)
                     /gene="cysS"
                     /locus_tag="PXA33_001886"
     CDS             complement(3809..5185)
                     /gene="cysS"
                     /locus_tag="PXA33_001886"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HDL5121195.1"
                     /translation="MLKIYNTLKREKEEFKPINPNQVGMYVCGVTVYDLCHFGHGRTF
                     VSFDVIARYLRYSGYNLRYVRNITDVDDKIIKRALENNETCDELVDRMIAEMHKDFDA
                     LNILRPDVEPRATKHIPEIIAMVEKLIHNGHAYVAADGDVMFDVESFQKYGALSRQNL
                     EQLQAGARVEIKSIKKNPMDFVLWKMSKAGEPSWESPWGNGRPGWHIECSAMNSKELG
                     EHFDIHGGGSDLMFPHHENEIAQSCCAHGGDYVNYWLHTGMLTIDKEKMSKSLGNFFS
                     IRHMLGLYDAESLRYFFLTAHYRSLLDYSIDNLDLARSALERLYTALRGCDVSGEAIG
                     EHYVEAFKTAMDDDFNTPGALAVLFEIARELNKLKTEDMAKANGLAVRLKELAGVLGL
                     LEQDPDAFLKGDANDDETAEIEALIKQRNEAKAAKNWAVADEVRDKLKAINIVLEDTP
                     NGTIWRKA"
     gene            5341..5850
                     /locus_tag="PXA33_001887"
     CDS             5341..5850
                     /locus_tag="PXA33_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDL5121196.1"
                     /translation="MITLHTNFGDIKIELNFEKAPITAKNFEDYAKEGFYNGTIFHRV
                     IDGFMIQGGGMTAGMAEKPTKDPIQNEANNGLSNKRGTIAMARTSDPHSASSQFFINV
                     ADNTFLDYRSKEMFGRSVVQDWGYAVFGEVVEGMDVVDKIKGVKTGNKGFHQDVPVED
                     VVIESVSVA"
     gene            complement(5942..6871)
                     /locus_tag="PXA33_001888"
     CDS             complement(5942..6871)
                     /locus_tag="PXA33_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HDL5121197.1"
                     /translation="MEIALLLADKILQLTLMVLLGYLIVKTKLLKSEHSYPISVIGLY
                     IISPAMLITAFQVDYTTEILNGLYLAFLISVLLNGLLILLGWLLKRALKLDNIEQATA
                     IYSNSGNLIIPIVASLFGNEWVIYSTAFIVVQNVLFWSHLRLLICGKGNVPFRKMIFN
                     INIIAIVIGMMLFLLHIKLPATLANTLSTLGHMLGPLAMLVAGMLIAAIPFKEIITDK
                     RIYLVAFLRLIFIPLILLAVIKLGGFASWIENGKTVVMITFLATISPSAATATQMAVA
                     YGQDAKKASAIYGVTTLLCVFTMPLIIWLYQFL"
     gene            complement(6874..7593)
                     /gene="fdhD"
                     /locus_tag="PXA33_001889"
     CDS             complement(6874..7593)
                     /gene="fdhD"
                     /locus_tag="PXA33_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012621653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HDL5121198.1"
                     /translation="MPVALVYNGISHTVMMATPANLEDFALGFSLAEGILNAPSELYG
                     LDVIEQENGVEIQLEISSRQFVALKDKRRSMAGRTGCGICGVEQLEQVNQSCKIVKKN
                     NRLQNVNPLILEDCLLQLENAQFLSKETGASHAAAFFTPDGKLLAIREDVGRHVALDK
                     LIGWYAKADFPIGFVFVTSRASFEMVQKCLAVGIEMLCAISATTEMAVNMARENQFTL
                     LAFARKGKATIYAGEERLALN"
BASE COUNT         2246 a         1624 c         1608 g         2125 t
ORIGIN      
        1 taattgtttt atatagaaag acaaagaaat ttaaaaaaat gtggtgaaaa acagcgttaa
       61 gcccattgac aaaggcatta ttttcacgca ttcttgaacc attattcact ctcttgaccg
      121 agattccgcc aatgaattgt tttaatatga tgacaacgat tattaccacc attatgtcac
      181 caacatagtg cgggttttaa ttggctgaaa taatgaaaga ataaaccgaa aacccgctac
      241 aagcgggttt ttttgtataa aaatttacaa ataatttatc aggagtaact atgcgagttc
      301 ttaaatttgg aggcacttct cttgccaacc ctgaacgctt tatgcaagcg gcagagatta
      361 tcgagaaagc tcatttggca gatcaagctg ccggcgtatt atccgcccca gccaaaatta
      421 ccaatcatct tgtcgctatt gtagataaag cgattgccgg tgaatcattt caatctaacc
      481 tttctgaagc taccgagatt ttccacaaca tcattaacgg cttatatgct acaaataatc
      541 actttgctaa agaagcactg ttagccttag tggaagcgga attaaaccaa attcagcaag
      601 tggctgcaga agcagctcaa actaaatcgt tgtcagataa aattgctgcc actattcact
      661 gccgtggtga aaaactctct attgcgatga tgcaagcgtg gttcgaggca aaaggctatg
      721 aagtcactcg tatcgaccct gttgaaaaat tattggctca cggcagctat ttggaatctt
      781 cggtcgatat tgccgaatcc accaagcgag ttgatgcagg ttcaattcca aagaaaaata
      841 tcgtgctaat ggctggtttt accgcaggta atgagcaagg cgaactggtg ttattagggc
      901 gtaacggttc agattattct gccgcttgtt tggcagcgtg tttaaaagcc gatgtttgtg
      961 aaatctggac ggacgtagat ggcgtttaca cctgcgaccc tcgtttggtg cctgatgcag
     1021 tatgcttaga aacaatgagc tatcaagagg caatggagct ttcctacttt ggggcgaaag
     1081 tgatccaccc tcgcaccatc gggccattag tgccgctcaa tattccttgt ttaatcaaaa
     1141 ataccggcaa tccggacgga aaaggcacat taattgatgg caatgtggca aacgataatc
     1201 tcaaagtaaa agggattacc aaccttgata acgtggcgat gttcaacgtt tcaggttcag
     1261 gaatgcaggg aatggtgggc atggctgccc gtgtgttctc gacgatgtca aaagccggta
     1321 tttcggtgat tttaattacc caatcatcat cagaatatag catcagcttc tgcgtgccgg
     1381 caaaatcggc agaaaaagcg ttagcggctc taaatactga atttgcacaa gagttatctg
     1441 aaaacttact tgacccgatt gatattatta aagatttatc cattgtttcg gtagtgggtg
     1501 atggtatgcg taccgccaaa ggaattgcgg ctcgcttctt ctctgcccta gcccaagcca
     1561 acatcagtat tgtggcaatt gcacagggtt catctgagcg ttccatttct gctgtcgtgc
     1621 cgttaaacaa agcgattgaa gcagtaaaag caactcacaa atcgctattt aacagtaaac
     1681 gtgtggtgga tatgttttta gtgggtgtag gcggtgtggg tagcgaactc atcgagcaag
     1741 tcaaacaaca aaaggagttt ttggcaaaga aggatattga gatccgtgtg tgtgctttag
     1801 ctaattcaga taaaatgtta ctcaacgaaa acggcttaaa tttagacaac tggaaagaag
     1861 atttagaaaa agcaattcag ccgtcagact ttgatgtatt gctttctttc atcaaattcc
     1921 accacgtggt aaacccagta tttgtagatt gcacttcggc acaatcggtg gcgaacctgt
     1981 acgcacgagc cttaaaagag ggcttccacg tggtaacacc aaacaaaaaa gccaatacat
     2041 caggttacga ttactactta gaaatgcgtg aaaacgcccg ccaaagccag cacaaattcc
     2101 tctacgaaac taatgtaggg gcaggtttac cggtaattga aaacttacaa aacctattag
     2161 ctgccggtga cgaagtagag aaatttgaag ggattttatc cggctcgctc tctttcattt
     2221 tcggcaaatt agaagaaggt ttaacccttt cgcaagcaac tttaatcgcc aaagaaaaag
     2281 gctttaccga gccagatcca cgagatgatt tatccggtgc agatgttgct cgtaaactct
     2341 taattcttgt tcgtgaaacc ggcttaccgc ttgaatttga agatattgaa attgaaggcg
     2401 tattgccaaa aggcttctcg gaaggaatga gtcgtgatga gtttttacaa gctctaccgt
     2461 ctttagacga agaattcagc aagcgtgtag aagctgccaa ggctgaaggc aaagtgttgc
     2521 gttatgtggg ctcgatcaca ggtgataaat gccgagttgc cattgaagcc gtggacgaaa
     2581 accacccact ttacaaagtt aaagacggcg aaaatgccct tgcttttctc acacgttact
     2641 acaaccctat tccattatta ttgcgtgggt atggtgcggg caatagcgta accgctgccg
     2701 gtatctttgc agatattctg cgtactttac aaggaggagt gaactaatgg cgttacgaat
     2761 ttatgcccca gcttcaagtg caaatattag tgtgggtttt gactcattag gcgctgcaat
     2821 ctccccgatt gacggctctt tgctcggcga tgtagttcaa attgaagaga gtgaacaaca
     2881 atttgagcta gaaagtgcag gttattttgt acgtaaattg cccaaagaac cacaaaaaaa
     2941 cattgtctat caagcctatg tgctatttag cgaacgcttg aaattacgta acggcagtgt
     3001 aaaaaattta cgcttaacac ttgaaaaaaa tatgccgatt ggctcagggc taggctcaag
     3061 tgcctgctcg attgttgcag cattagtggc actcaataaa ttccacgatg aaccgttctc
     3121 caaaatggaa ttactggaaa tgatgggcga attggaaggc agaatttcag gctcaatcca
     3181 ctacgacaac gttgcccctt gctatttagg tggcctgcaa ttaatgacac aatcgctcgg
     3241 caatatttgc caacctctgc cattctttga tgaatggtat tgggtgcttg cctacccggg
     3301 cgtggaagta tcaaccgccg aagcccgtgc tattttgccg aaaagctata ctcgtcaaga
     3361 tatgattcag caagcccgct acttaggatc tttcgtccat gcttgccata cacatcaaga
     3421 tatcttggct gctacaatga tgaaagacct aattgctgag ccttaccgtg aagatttact
     3481 gccgaacttt ccggctgttc gtcaaggctg taaagactta ggtgcgttag cggttggtat
     3541 ttcaggttcc ggtccgacaa tgtttgcgat agcccctgat ctcgaacacg ctcaacgttt
     3601 agtgagctat ttagagaaag aatatctgca aaacaacgaa ggctttattc atatctgtaa
     3661 agtcgataat caaggagcaa gagagctgaa atagccctct cgaacaacca tatcactaaa
     3721 taacttaaag gactgactcc aataccactt ggagcagccc tttttgatta attttttgaa
     3781 aagtctatca cttataaaca atcgaaggct atgccttacg ccaaattgtg ccgtttgggg
     3841 tgtcttcgag gacaatattt atcgctttga gcttatctcg cacttcatcg gcaaccgccc
     3901 agtttttagc cgctttggct tcgttgcgtt gtttgattaa ggcttcgatt tctgccgttt
     3961 catcgtcatt cgcatcacct tttaaaaacg catcggggtc ttgttccagt aagcctagca
     4021 cgccggctaa ctcttttaaa cgaacggcta aaccgttggc ttttgccata tcttcggttt
     4081 tgagtttgtt caactcacgg gcaatttcaa ataacaccgc taatgccccc ggggtgttga
     4141 aatcatcgtc cattgcggtt ttaaaggctt ctacatagtg ttctccgatg gcttcgcccg
     4201 acacatcaca accacgcaga gcggtatata aacgctcaag agccgaacgg gctaaatcaa
     4261 ggttgtcaat gctgtaatct aataacgaac ggtaatgagc ggttaagaag aaataacgta
     4321 acgattcggc atcgtataag cctaacatat ggcgaatgct gaagaaattg cccaaagatt
     4381 tcgacatttt ttctttatca atggtaagca ttccagtgtg taaccaataa ttgacataat
     4441 cgccaccgtg agcacagcaa gattgtgcaa tttcgttttc gtgatgcggg aacattaaat
     4501 ccgagccacc gccgtgaata tcaaaatgtt cgcccaactc tttgctattc attgccgaac
     4561 attcaatatg ccagcccggt ctgccattgc cccacgggct ttcccagctt ggctcaccgg
     4621 ctttcgacat tttccacaag acaaaatcca tcgggttttt cttgatggat ttaatttcaa
     4681 cccttgcacc ggcttgtagt tgctctaaat tttggcggga taatgcacca tatttctgga
     4741 acgattccac atcaaacatc acatcgccat cggctgcgac ataagcgtgt ccgttgtgaa
     4801 tcagtttttc taccatcgca ataatttcag gaatatgctt ggtggctcgt ggctcaacgt
     4861 ccgggcgaag aatgtttaac gcatcaaaat ctttgtgcat ttcagcaatc attctatcca
     4921 ctaattcatc gcaagtttcg ttattttcta acgcacgttt gatgattttg tcgtccacat
     4981 cggtaatgtt acgcacatag cgtaaattgt agcctgaata acgtaaataa cgggcaatca
     5041 catcgaaaga gacaaaagtg cgaccgtgcc caaaatgaca aagatcgtaa accgtgacgc
     5101 cacagacgta cattcccacc tgatttgggt taatcggttt aaattcttct ttttcacgtt
     5161 ttaaggtgtt ataaattttg agcataaagt cctcttgtgt aacattcagt aacaaatggg
     5221 gctaagtata gcgaataagc ggtctgattt tgagattttt ttgcaatttg gcgtaaactt
     5281 tttaataata gcgagcgaaa tttattttcc attccatttt tatttataaa ggaacaaaga
     5341 atgattacat tacataccaa tttcggtgac attaaaatcg aattaaactt tgaaaaagcc
     5401 ccgattaccg ctaaaaactt tgaagattac gctaaagaag ggttttataa cggcacgatt
     5461 ttccaccgtg tgattgacgg ctttatgatt caaggtggcg gaatgacggc aggtatggct
     5521 gaaaaaccga caaaagatcc gattcaaaac gaagcgaaca acggtttaag caacaagcgt
     5581 ggcacaatcg caatggctcg cacttctgat ccgcattctg cttcgtcaca gtttttcatt
     5641 aatgtggcgg ataatacttt cttagattat cgctcaaaag agatgttcgg cagaagtgta
     5701 gtgcaagatt ggggctatgc agtatttggc gaagtggttg aaggtatgga cgtggtcgat
     5761 aaaatcaaag gcgtaaaaac cggcaataaa ggcttccacc aagacgtgcc ggttgaagat
     5821 gtggtgattg agtctgtaag cgttgcctaa ttccgcaaat tagcctacaa gcggttgatt
     5881 tttcacaatt ttttgcattt ctcagcgttg caaaaaagta gcagaaatcg actgcttgtt
     5941 tttatagaaa ttgatacagc caaataatca gcggcattgt aaacacacaa agtaaagtgg
     6001 ttacgccata aatcgcactg gcttttttgg catcttgccc gtaagccacc gccatttggg
     6061 tcgccgttgc agcagaaggg ctgatggtgg ctaagaatgt aatcattacc accgtttttc
     6121 cattctctat ccagctcgca aaaccgccga gtttaatcac cgctaacaaa atcagcggaa
     6181 taaaaatcaa gcggagaaaa gccaccaagt aaatgcgttt atcggtaata atctctttaa
     6241 atggaattgc tgcaattaac atccctgcca caagcattgc taaaggccct aacatatgcc
     6301 ctaaagttga gagcgtgttt gcaagcgtag cgggcagttt gatatgtagc aaaaacaaca
     6361 tcataccaat cacaatcgca ataatgttga tattaaagat catctttcta aacggcacat
     6421 tgcctttgcc acaaatcagc agccgtaagt gagaccaaaa caacacattt tgcaccacaa
     6481 taaaagccgt tgaataaatc acccattcat tgccgaacag ggaggcaaca atcggaataa
     6541 ttaagttgcc ggagttggaa taaatagcgg tggcttgttc tatgttgtct aatttcaacg
     6601 ctcttttgag cagccaacca agcagaatta acaagccgtt taataagacg gagattaaaa
     6661 atgccagata caagccgttt agtatttcgg tggtgtaatc tacttgaaaa gcagtaatca
     6721 gcattgccgg gctgataatg taaagtccga taaccgaaat cggataactg tgctccgatt
     6781 tgagtaattt agttttaacg atgaggtagc cgagcagcac catcagggtt aattgtagaa
     6841 ttttatcggc taataaaagg gctatttcca ttatcaatta agagcgagtc tctcttctcc
     6901 tgcataaatg gtcgctttgc ctttgcgggc aaaagcaagt aaggtaaatt gattttcacg
     6961 agccatattc actgccattt cagtggtcgc tgagatggca cacaacattt caatgccaac
     7021 ggctaagcat ttctgcacca tttcaaagct ggcacgactg gttacaaaca caaatccaat
     7081 cggaaaatca gctttagcat accaaccgat taatttatcc aacgccacgt gtctgcccac
     7141 atcttcacga atggcaagca gttttccatc cggggtaaaa aatgcagcgg cgtggcttgc
     7201 accggtttct ttcgataaga attgagcatt ttctagctgt aataaacaat cctctaaaat
     7261 cagtggattg acgttctgca agcggttatt tttcttaaca attttgcaac tttgattaac
     7321 ctgctctaat tgctctacac cgcaaatacc gcaacctgtt ctgcctgcca ttgagcggcg
     7381 tttatctttt aatgcaacaa attggcggct cgatatttca agctgaattt ctactccatt
     7441 ttcttgttca attacatcta aaccatataa ttccgatggg gcatttaaaa tcccttccgc
     7501 caatgagaat cctaaagcga agtcctccaa attagccggt gttgccatca ttacagtatg
     7561 agaaataccg ttatatacca aagcaaccgg cacttctgaa att
//
LOCUS       DANLRK010000121         7553 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.121, whole genome shotgun sequence.
ACCESSION   DANLRK010000121 DANLRK010000000
VERSION     DANLRK010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7553
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.121"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            <1..708
                     /locus_tag="PXA33_001890"
     CDS             <1..708
                     /locus_tag="PXA33_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005615381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDL5121199.1"
                     /translation="LPPFNIILLWLLALILFSLGYKYAAYFFALLGILILYIFSIPYT
                     SQKLGDSLVEAEPFTIEKYKNAQAIVVLGGGLRDSQELFGKLAITGIPLERMRYAAYL
                     HKETGLPILATGSSPNGTSEAKVMADEFKMFFNIETKWLENKAKTTKENAIFSREILE
                     KENIHKIILVTNQWHMKRAEMLFSQQGFEVMSASVGSGDTPDHYKLNFMHFIPQAGAM
                     NSNMLALKEWIGYWKEK"
     gene            complement(785..2983)
                     /gene="priA"
                     /locus_tag="PXA33_001891"
     CDS             complement(785..2983)
                     /gene="priA"
                     /locus_tag="PXA33_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HDL5121200.1"
                     /translation="MALNVPLHRYFDYLLPPAFNACQGGRVVVPFGRQTKIGIVVDFP
                     QTSDVPTEKLKPIKAALDSSPIFDSEMWQLLNWAARYYHAPIGEVLTGALPIKLRNGD
                     STERVQPNYFVVTEQGRKSLIIGENKKAKKQVERLTELAECAKYFEKPTACSPSAWKG
                     LLEKGYIEKIDVPFMAQAWQDSLGEFPLVNTNNRLILNKQQTLVVSRLNAQTTFASFL
                     LNGVTGSGKTEVYLQLIETVLKREEQVLLLVPEIGLTPQTVQRFKARFNVEIDVLHSN
                     MNETERLNAWLRAKNGESAIVIGTRSALFTQFQKLGVIILDEEHDSSFKQQDGWRYHA
                     RDLAVVRAKNANIPIVLGSATPSLESLHNVQNGKFIELTLTARAGNAQLANQQIIDLK
                     TQRVTAGLSERLLSMMKTQLEQGNQVMLFLNRRGFAPVLLCHECGWVCECEACNKPFT
                     YHQKQKVLRCHHCATQRVIPRQCGHCGSTHLITTGVGTEQLEQVLNQQFPHYQVTRID
                     RDSTARKGALENHLTAIREGKSQILIGTQMLAKGHHFPNVTLVAIVNVDSALFSTDFR
                     AEEKLAQLYIQVAGRAGRAEKKGEVVLQTHYPDHPLLKTLLEQGYRAFADEALKMRQL
                     MSLPPFSSQVLFKATGKDNQQTLQLLEQLADYFSQKIQQQNGQGFQLPPPFSAPMAKK
                     AGHYRWLLLIQHPLRGALQQLLDAFDQERESLNIPNNIRLNIDIDPQEIS"
     gene            complement(2995..3607)
                     /locus_tag="PXA33_001892"
                     /pseudo
     CDS             complement(2995..3607)
                     /locus_tag="PXA33_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005818858.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
     gene            complement(3600..4499)
                     /gene="rarD"
                     /locus_tag="PXA33_001893"
     CDS             complement(3600..4499)
                     /gene="rarD"
                     /locus_tag="PXA33_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HDL5121201.1"
                     /translation="MLKGILLSLSASVLFGCMYYLAIFLKPLAGVSIFGIRMLVTIPF
                     LFLALYLFKKQNEFWVFLQRLKQEPYLLLVILITASIVGGQMWLFLWAPNSGKAIEVS
                     MGYLLMPIIMVAFGKVVYKETLSLNKWLAIVVAFIGVASNILFTGKISIASLFVCTGY
                     PIYFYLRRKFGLSHLHSFVVEILYLLPVACYFISQTDMQYVETQNLNIYYFIALLGLI
                     SGTALISYTLASTILPFNLLGLLGYVEPLLMLVVSFLIGERLANESYLLMICLLISIS
                     FLVLDGMFALRQQRKRKLSVNNE"
     gene            complement(4726..5943)
                     /locus_tag="PXA33_001894"
     CDS             complement(4726..5943)
                     /locus_tag="PXA33_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDL5121202.1"
                     /translation="MDRFPKSDKLAQVRYDIRGPIHKEALRLEEEGHKILKLNIGNPA
                     PFGFEAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIVQYYQSKGMRGMDVNDVYIGN
                     GVSELITMSMQALLNDGDEILIPMPDYPLWTAASTLAGGKAVHYLCDEENEWFPDLED
                     IKSKITPRTKGILVINPNNPTGAVYSRQILLEIAELARQHNLIIFADEIYEKIVYDGA
                     VHHHIAALAPDVLTVTYNGLSKAYRVAGFRQGWMVLSGPKHQAKGFIEGLDMLSSMRL
                     CANTPMQHAIQTALGGYQSINEFVLPGGRLLEQRNKMHELLVQIPGISCVKAKGALYM
                     FPKIDTEMYGIKDDQKFIYDLLRQEKVLLVQGSGFNWKKPDHFRVVTLPYAHQIEEAI
                     GRLANFLKTYRQD"
     gene            6131..7435
                     /locus_tag="PXA33_001895"
     CDS             6131..7435
                     /locus_tag="PXA33_001895"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase"
                     /protein_id="HDL5121203.1"
                     /translation="MLIFHQLKQELTKSYSKVGREQGLVAIQASMPLLEANIPLLGWL
                     KTQKTSYPHFFLQYRDTDQTIATIGTLRQFYTLEAAQSFIQQHHFPLIGGLQFEGNTQ
                     FILPQFSLVKNQQNLTACFYFDSDNFEQQAVIFEQFLANFSQITELGLTDNPLVSMSA
                     TSHFEQWKANIEKAIVSIEQGDFQKVVLANAMRLHFEQPICAYSLLAQSQKTNLGCYH
                     FLWAESADVAFLGSTPERLYQRQGLHFSTEALAGTAAVTTSEVQTELNAQWLLSDPKN
                     IYENQLVVDDIENQLKDCVTEFAVSEAKIKRLHNVQHLRRQIQATLKPDTSDSDCLSK
                     IHPTAAVAGLPRQKAKGFITENEPFTRHWYAGTLGVMGNEEAEFCVTLRSALISQNSI
                     TLYAGAGIVSGSDPQSEWQEIKRKAQGMAKLLDVDLVTKGEL"
BASE COUNT         2285 a         1655 c         1484 g         2129 t
ORIGIN      
        1 ctcccgccat ttaatattat tttgctgtgg ttgttagcat taatcctctt ttctttaggc
       61 tataaatacg ctgcttactt ctttgccttg ttaggtattc tgattttata tatcttcagc
      121 attccctata cttcacagaa attaggggat tcattagtcg aagcagagcc atttaccata
      181 gagaaatata aaaatgctca agcgattgtg gtgctaggtg gcgggctacg agacagccaa
      241 gagttattcg gcaaactggc aattaccggt attccgctgg aaagaatgcg ttatgccgct
      301 tatctgcata aagaaaccgg cttaccgatt ttagcgaccg gaagcagccc aaatgggact
      361 tctgaggcaa aggttatggc agatgaattt aaaatgttct tcaatattga gaccaaatgg
      421 ctggaaaata aggcgaaaac caccaaagaa aatgcgattt tcagccgtga gattttagaa
      481 aaagagaata ttcataaaat cattcttgtt accaaccaat ggcatatgaa acgggctgaa
      541 atgctgttta gccaacaagg atttgaggtg atgtctgcca gcgtcggttc tggcgatacg
      601 cccgatcatt acaaactcaa ctttatgcac tttattccgc aagccggtgc aatgaatagc
      661 aatatgctgg cattaaaaga gtggattggt tattggaaag agaaataacc gaagataaat
      721 agcagcaagg caggttttca cctgccttct ttttgcaaaa aatcagtcaa aaacaaccgc
      781 ttgtctaact aatctcttgt gggtcaatgt cgatattcag cctgatatta ttcggaatgt
      841 ttaaactttc cctttcttga tcaaaggcat cgagtagctg ttgtaatgct cctcggagag
      901 ggtgttgaat cagcaataac cagcggtaat gtcctgcttt cttcgccatt ggggctgaaa
      961 atggtggagg gagctggaag ccttgcccgt tttgttgctg gattttttgg ctaaaataat
     1021 ctgctaattg ctcaagcagc tgtaaggttt gttgattatc tttgccggtc gctttgaaca
     1081 aaacttgcga ggaaaatggt ggcaaactca ttaattgcct catttttaac gcctcatcag
     1141 caaaagctcg gtagccttgt tccagcaagg ttttaagcag tgggtgatca gggtaatggg
     1201 tttgtagcac cacttcgcct tttttctccg ctcgccctgc tctgcctgcc acctgtatgt
     1261 aaagctgtgc taatttttct tccgcacgaa aatcagttga aaataacgct gaatccacat
     1321 taacaatcgc caccaacgtc acattcggga aatgatgccc ttttgccaac atttgcgtac
     1381 caatcaaaat ttggcttttg ccctctcgaa tagcggtcaa atggttttcc aatgcacctt
     1441 tgcgagcggt agaatctctg tctatacgag tcacttggta atgagggaat tgctgattta
     1501 gcacctgctc aagttgttct gtgccgacac cggtagttat taaatgggtt gaaccacaat
     1561 gtccgcattg gcggggaatt accctttggg tggcacaatg atggcaacgc aacacttttt
     1621 gcttttggtg gtaagtaaaa ggcttattac acgcctcgca ctcacaaacc caaccgcatt
     1681 cgtgacaaag taataccggg gcaaagccac ggcgatttaa aaacagcatc acttgattgc
     1741 cttgctctaa ctgggttttc atcattgaaa gcaagcgctc cgacaaaccg gcagttaccc
     1801 gttgcgtttt taaatcaata atctgctgat tggcaagttg tgcattgccg gcccgagcgg
     1861 ttagtgtcag ttcgataaat ttcccgtttt gcacattgtg caaactttca agacttggcg
     1921 ttgccgagcc cagcacaatc ggaatatttg catttttcgc cctaaccacc gccaaatccc
     1981 gtgcgtggta acgccaaccg tcttgttgct taaacgagct atcgtgttct tcatccaaga
     2041 taatcacgcc caatttttga aactgggtaa ataaagcaga gcgagtgccg attacaatcg
     2101 cactttctcc atttttcgcc cgcaaccacg catttaaccg ctcagtttcg ttcatattcg
     2161 agtgcaatac gtcaatttca acgttaaagc gagctttaaa gcgttgtacc gtttgcggtg
     2221 ttagcccgat ttcaggcaca agcagcagca cttgttcctc tcgcttaagc accgtttcaa
     2281 ttaactgcaa atacacttcg gttttgcccg agcctgttac gccattgagt aaaaagctcg
     2341 caaaagtggt ttgtgcattt aaacggctga ccactaaggt ttgttgttta tttaagatca
     2401 gccgattgtt agtatttact aacggaaatt cacctagtga gtcttgccac gcttgtgcca
     2461 taaacggcac atcaattttc tcgatatacc ctttttcgag taagcctttc caagcagatg
     2521 ggctacaagc ggtcggtttt tcaaaatatt ttgcacattc ggcaagctcg gttaaccgtt
     2581 ccacctgctt tttggctttt ttattttcac cgataatcag agatttccgc ccttgctccg
     2641 tcaccacaaa ataattcggt tgaactcgct cagtggaatc gccattgcgt aattttatcg
     2701 gtaaggctcc ggttagcact tcaccaatcg gggcgtggta ataacgagcc gcccagttca
     2761 gcagttgcca catttctgag tcaaaaatcg ggctagaatc caacgctgct ttaatcggtt
     2821 tgagtttttc ggttggcaca tcactagttt gcgggaaatc gaccacaatg ccgattttgg
     2881 tctggcgacc aaaaggcaca accacccgcc cgccttggca ggcattgaat gccggcggta
     2941 gcaaataatc aaaataacga tgtaacggca cattcagtgc cactctgatt aatctcactt
     3001 ctctaaacca attttgattg caagcccgat aaatatcgag ccgcagattt tatttaacca
     3061 atttccaaag gtttgattat gccgtaaaaa acgcccaaca ggtgcagcgg ctaagactaa
     3121 aatacttacc caaataatgc tggtggataa aaacgttagc cccaacagca taaaaggtat
     3181 tttccaaatt actggataca aactgcggca ataacgccaa gaaaaagagc aatactttcg
     3241 ggtttaatag attggtaatc aacccttgac ggaatatctt ccatagagga agtttgtcta
     3301 gattgatttt ctctaacacc aacggctctc gccacatttt aatgccgaga tagcagagat
     3361 aaattgcccc tgcgtatttc agaaaattaa ataaagtggg cgaatgtgcc acaatgcctg
     3421 caagcccgag tgaaaccgca agaatatggc ataaaatgcc tgctagaatg ccgaatgaag
     3481 acattaagcc cgactttttc ccctcagcaa tagtgcgagt taaaatatag atagtatccg
     3541 aacccggtgt aagatttagc agaatacaag cggtcaaaaa tccccaataa tttacaatat
     3601 cactcattat tcacactcaa tttacgttta cgctgctgac gaagagcaaa cataccatct
     3661 aatactaaaa atgaaatgga gatcaacaag caaatcatca acaagtaaga ttcatttgct
     3721 aacctttcgc caattaagaa agacaccacc aacatcaata gtggctcaac atagcctaat
     3781 aaaccaagca aattaaaggg taaaatcgtg ctggctagcg tatagctgat aagtgcagtg
     3841 ccactaatta aacccagtaa tgcaataaaa tagtaaatat tgaggttttg tgtttcgaca
     3901 tattgcatat cggtttgaga gataaaataa caagctacag ggagcaaata taaaatttct
     3961 accacaaagc tatgtaaatg gcttaagcca aactttcggc gaaggtaaaa atagattggg
     4021 taacctgtgc aaacaaataa acttgctatc gaaatttttc ccgtaaacaa aatattactc
     4081 gctacaccaa tgaaagcaac cacaatcgcc aaccatttat tcaaggaaag cgtttcttta
     4141 taaaccactt tgccaaatgc caccataata atcggcatta atagatagcc cattgaaacc
     4201 tcaatcgctt ttccgctatt gggagcccat aaaaacagcc acatctgccc gcccacaata
     4261 gatgccgtga ttaaaataac cagcaataaa taaggctctt gctttaggcg ttgaagaaat
     4321 acccaaaact cattttgttt tttaaataag tataaagcaa ggaaaagaaa aggaatcgta
     4381 accagcatac gaatgccgaa aatgctcaca cctgccagcg gttttagaaa gatcgccaaa
     4441 tagtacatac agccgaataa cactgaggct gaaagagata aaagaatgcc ttttaacata
     4501 gtgataagaa agtaattatt ttttaatttt tcaaatctcc cctacccctc ttttctaaag
     4561 aggattgtcg agttctaaat aaatgaataa tgttcggagg taggggcggg tccagtgccc
     4621 gcccgcaaat taatatcgtt agcggacagg cacaggacct gtccctactg atattataga
     4681 acttacaacc ctgcttttat aaagtggggt aaagggagat tcgacttagt cttgacgata
     4741 ggttttcaag aaattcgcaa ggcgaccaat cgcttcttca atttgatgtg cataaggcaa
     4801 agttaccaca cggaagtgat ccggtttttt ccagttaaaa ccggaacctt gcactaatag
     4861 cactttttct tgtcgcaaca aatcgtagat gaatttttgg tcatctttaa tgccgtacat
     4921 ttcggtatca attttcggga acatatataa cgcccctttc gcttttacac agctaatacc
     4981 cggaatttgt accaataatt cgtgcatttt atttcgttgt tccagcaaac gaccgcccgg
     5041 caacacaaat tcattgatac tttgataacc acctaatgcg gtttgaatgg cgtgttgcat
     5101 cggcgtattg gcacataaac gcatagaaga gagcatatcc aacccttcaa taaaaccttt
     5161 ggcttgatgc ttcggaccgc tcagcaccat ccagccctga cggaaaccgg caacacgata
     5221 cgccttggat aaaccgttat aagttacggt taatacgtcc ggtgcaagag cagcaatatg
     5281 gtgatgtacc gcaccatcat agacaatttt ttcataaatt tcgtcagcaa aaataattaa
     5341 gttatgctga cgggcaagtt ctgcaatttc cagcaaaatt tgacggctat acactgctcc
     5401 ggtcgggtta ttcgggttga taaccaaaat ccctttagtg cgaggcgtga ttttcgattt
     5461 aatatcttca agatcaggga accactcatt ttcttcatcg cacaggtaat gcactgcttt
     5521 accaccggct agggttgaag ctgctgtcca taacggataa tcaggcattg gaattagaat
     5581 ttcatcgcca tcatttaata acgcctgcat cgacattgta atcagttcag atacgccgtt
     5641 gccgatgtaa acatcgttta catccatacc acgcatacct tttgattgat aatattgcac
     5701 aatcgctttg cgggcagaat acaaaccttt tgaatcacaa tagccttgtg cggttggaag
     5761 attacgaatc acatccacta aaatttcatc gggtgcttca aagccaaaag gggctgggtt
     5821 accgatattt aattttaaaa ttttatgccc ttcttcttct aagcgaagtg cctctttatg
     5881 aattggccca cggatatcgt aacgaacctg agctaactta tctgatttgg gaaaacgatc
     5941 cattgtaaac ttcctataat gtattatcta attgtagtct catcccgctg ttcaaaaaat
     6001 cggtaattcg gaatgaattt gtaaacatta ctctaatttg ggaggaaaaa aaagaggaaa
     6061 tacgttaaaa tggagacctt tttttcaatc agctaaaaac aagcggtttc tttttctgat
     6121 ttttttgcaa atgcttattt ttcatcaatt aaaacaagaa cttacaaaaa gttactcaaa
     6181 agtgggaaga gagcaaggat tggtcgccat tcaagcctct atgccgctat tggaggcaaa
     6241 tattccgcta ctcggttggt tgaaaacaca aaaaacaagc taccctcact tttttctgca
     6301 atatcgtgat acagatcaga ccattgccac cattggcaca cttcgccaat tttatacgct
     6361 agaagctgca caatctttta ttcaacaaca tcatttcccg ttaatcggcg ggcttcaatt
     6421 tgaaggcaat acgcaattta ttttgcctca attttcattg gtaaaaaatc agcaaaattt
     6481 aaccgcttgc ttctattttg atagtgataa ctttgaacaa caagcggtta tttttgaaca
     6541 atttcttgca aatttcagcc aaattaccga attaggacta acggataatc cgctcgtttc
     6601 aatgagtgcc accagccatt ttgagcaatg gaaagccaat attgaaaaag cgattgtttc
     6661 tattgagcag ggcgattttc aaaaagtggt attggcaaat gcgatgagac ttcattttga
     6721 gcaacccatt tgtgcttaca gcttgctggc acaaagccaa aaaacaaatt taggttgtta
     6781 ccacttttta tgggcggaaa gtgctgatgt tgcctttctt ggctcaaccc ctgagcggct
     6841 atatcaacgc caagggctac acttttctac cgaagccctc gccggcaccg ctgcggttac
     6901 caccagtgaa gtgcaaaccg aactcaatgc acaatggctg ttaagtgatc ccaaaaatat
     6961 ctatgaaaat caattagtgg ttgatgacat tgaaaaccaa ctcaaagatt gcgtgaccga
     7021 atttgcagta tccgaggcaa aaattaagcg tttgcataat gtgcaacacc tacgccgcca
     7081 aattcaagca acgctcaaac cggacacctc agattcagat tgcttaagca aaatccaccc
     7141 aactgcggca gttgcagggc ttccccgcca aaaagcaaag ggttttatta ccgaaaatga
     7201 gccttttacc cgccattggt atgcaggtac attaggcgta atgggcaacg aggaagccga
     7261 attttgcgta acactccgtt ctgcattaat ttcacaaaat tcaattacgc tttatgccgg
     7321 tgccggcatt gtgtcaggct cagatccaca atcggaatgg caagaaatta aacgtaaggc
     7381 acaaggaatg gcaaaactgt tggatgttga tttagtaaca aaaggggaac tgtaagctag
     7441 gctttcagaa cttaatctct taattatttc tttctatatt ttcatttcca acttcccaac
     7501 aaaatatatt attgccacca aattagtagt gaatattcca taattaaaaa att
//
LOCUS       DANLRK010000122         7543 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.122, whole genome shotgun sequence.
ACCESSION   DANLRK010000122 DANLRK010000000
VERSION     DANLRK010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7543
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.122"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(18..1301)
                     /locus_tag="PXA33_001896"
     CDS             complement(18..1301)
                     /locus_tag="PXA33_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL5121204.1"
                     /translation="MTACHEPKRSIWQQIFTKNMLLCVYTGFCSGLPLFVLIQLMPAW
                     LTSANLDIKTIAAFTLTSLPYTWKFLWAALLDRYFPPFLGRRKSWIFISQIGLLVILA
                     SFGLFNPVQDISIIVTLSVLLAFLSATQDIVVDAFRREILSDNELGLGNSIHVNAYRI
                     AGLIPGGLSLFLADHYAWDTVFLITAAFLVPCLFVTLIIAAEPNHKPIDRSKPFYMAF
                     VDPFKEFFTRKGVAGAIGLILFIFLYKLGDSLATTLQTKFILDMGFTKSHIAGIVKIT
                     SLWCSIGGGIIGGVAMLKLGVNRALWLFGFVQLITILGFAYLASFEHFPTESIGAAEL
                     WKLGLVMAGEYLGVGLGTAAFVAFMARETNPAYTAMQLAIFTSLAALPSKMLGAYTGH
                     LVEMMGYYQFFWLCFFIGIPGMLMLFKVAPWGQAE"
     gene            1467..5183
                     /gene="metH"
                     /locus_tag="PXA33_001897"
     CDS             1467..5183
                     /gene="metH"
                     /locus_tag="PXA33_001897"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HDL5121205.1"
                     /translation="MNTQHRTELLQNALTERILILDGAMGTMIQKYKLTESDFRGERF
                     KNSTIDLKGNNDLLTLTQPLLISAIHEKYLAAGADIIETNTFSATTIAQADYELESIA
                     YELNLVGAKLARLAADKFSIPEKPRFVAGILGPTNRTCSISPDVNDPGFRNITFMQLV
                     EAYAEATRGLIEGGADLIMIETIFDTLNAKAAVFAIETVFDELGVKLPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAKPLTFGLNCALGPKELRPYVEMMSKISETFVSVHPNAG
                     LPNAFGGYDLGAEEMATYIKEWAELGWLNIVGGCCGTTPEHIQAFAEATKGVAPRKLP
                     EIPTAMRLSGLEPLTIDDNSLFVNVGERNNVTGSAKFKKLIKEEKFAEAIEIAISQVE
                     NGAQVIDVNMDEALLDSKKCMTRFLNILATEPEAAKVPIMIDSSKWEVIEAGLQTVQG
                     KPIVNSISLKEGEEKFIHQAKLCRRYGAAVVVMAFDEVGQADTEERKVEICTRAYRIL
                     VDQLGFPPEDIIFDPNIFAIATGIEEHNNYGVDFIQACERIKRDLPHAKISGGVSNVS
                     FSFRGNNVVREAIHAVFLYYAIKAGMDMGIVNAGQLAIYDDLDPELREAIEDAVLNHR
                     HDTTDRLLEISEKYRGVKVESADESAAEWRNLPVAERLKHALVKGITTYIIEDTEEAR
                     QQFASPLEVIEGPLMAGMDVVGDLFGDGKMFLPQVVKSARVMKQSVAYLEPYINATKQ
                     KGSSNGKMVIATVKGDVHDIGKNIVSVVLQCNNFEVIDLGVMVPADVIIETAIREKAD
                     LIGLSGLITPSLDEMEYFLGEMNRLKLDIPVLIGGATTSKEHTAIKLYPKYNYPVVYT
                     ANASRCVTVCATLMNPEAKAEFWEKTKAEYEEIQRKFAVRKPLRNGLSIEEARANRFD
                     GFSGEWADYVPPTPKQTGIIEYKNVPIATLRKFIDWSPFFMLWGLMGGYPDAFDYPEG
                     GEEARRVYNDAQRVLDEFEQNGKLNPSGIMGIFPAVREGDDIAILNEDRTACIGKSYH
                     LRQQSERGKNSKSPYNLCLSDFIADRESGQQDWFGMFAVCAGIEEHYLVESYKAAGDD
                     YNAILLQAVGDRLAEAMAEYLHFELRTKVWGYTVEDEDNGRLIREEYIGIRPAPGYPS
                     CPEHTEKQIIWDLLEVEQRIGMKLTESYAMWPAASVCGWYFTHPTSNYFTLGKIDEDQ
                     VLDYAKRKGWDELTAKKWLSVAMKQDVKSQAENI"
     gene            5291..7294
                     /locus_tag="PXA33_001898"
     CDS             5291..7294
                     /locus_tag="PXA33_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HDL5121206.1"
                     /translation="MIEKQNYHLVQLLKDSNYQLTQFSPAYIKKLEESIFLKSGKNGD
                     IPHIQCLVRKKDIKLTPEEAVRQLYLMNLHYEYDYPFERMELEYSVVFGREKKRADIV
                     IFDRDNPNAVYIMVELKKPKLKDGKEQLKSYCNATGAPIGVWTNGESISYYNRRDPNY
                     FKDIPDIPNAQQKLSDLLQERWTIQDLIDNDKIAKQRKSLKDLVIEMEDEVLANAGVD
                     VFEELFQLIFTKLYDEMESTRNKSRYLEFRNYGDTESELKAKIQSLFDRAKNKWEGVF
                     AESAKINLSPSHLAICVSSLEEVKLFNSNLDVVDEAFEYLINKSSKGEKGQYFTPRYV
                     IDMCVKMLDPKPEETVIDTAAGSCGFPVHSIFHVWEKQLKARGLERSHLFTAEEKLPE
                     QTDYVKEKVFAIDFDEKAVRVARTLNLIAGDGQTNVLHLNTLDYERWEDFTKEEEWND
                     VYGEGWKKLRKLRKTKNENRDFQFDVLMANPPFAGDIKETRILARYELGKNSKGKQQS
                     KVGRDILFIERNLDFLKDGGRMAIVLPQGRFNNSSDKYIRDFIAERCRILAVVGLHGN
                     VFKPHTGTKTSVLFVQKWHDELCPKVEDYPIFFATMQKSSKDNSGDKIYVKNADGTLK
                     LDNHQHLIVEHDLFNHDNSTQDGIAEAFIEFAKKEKLSFVGKD"
BASE COUNT         2334 a         1579 c         1735 g         1895 t
ORIGIN      
        1 cttattttta tgaatagcta ttcagcttgt ccccaaggag caaccttaaa caacattagc
       61 attcccggaa taccaataaa gaaacagagc cagaagaatt ggtaatagcc catcatttcc
      121 actaaatgcc ctgtgtatgc ccctaacatt ttgcttggta gggcagcaag gctggtgaaa
      181 atggcgagct gcattgcagt gtaagcggga ttagtttcac gagccataaa ggcaacgaaa
      241 gccgctgtgc ctaaacccac gcccagatat tcacctgcca tcactaagcc taatttccaa
      301 agctcggcag ctccaatgct ttcggtaggg aaatgctcaa aactggcaag gtaagcgaag
      361 cctaaaatag taattaattg tacaaatccg aacagccata aggctcggtt aacgcccaat
      421 ttcaacattg ccacgccacc aataataccg ccgccaatgc tgcaccaaag tgaagttatt
      481 ttcacaatgc cggcaatgtg cgatttagta aaacccatat ccaaaataaa tttggtttgt
      541 agcgtagttg caagggaatc gcctaattta tataggaaaa taaatagaat cagcccaatc
      601 gctcctgcca cgcctttacg ggtgaaaaac tctttgaacg gatcgacaaa cgccatataa
      661 aacggcttag aacggtcgat tggcttatga ttcggctcgg cagcgattat tagcgtaacg
      721 aataagcaag gcactaaaaa agcagcggta atcagaaata cggtatccca cgcatagtga
      781 tcggctaaaa ataatgaaag cccacccgga atcagccctg caatacgata agcattaacg
      841 tggatagagt tacctaaccc gagttcgtta tccgataaaa tttcacgccg aaaggcatcc
      901 accacaatgt cttgcgtggc ggaaagaaaa gctaatagca cagaaagagt aacaataatt
      961 gaaatatctt gcactgggtt gaacaagcca aagctggcta aaatcaccaa tagcccgatt
     1021 tgtgaaatga aaatccagct tttgcgtctg cctaaaaaag gtggaaagta gcgatctaac
     1081 aatgcagccc acaggaattt ccacgtgtag ggcaaagagg tcagggtgaa ggcggcaata
     1141 gttttaatat ctaaattggc actggttagc caagccggca ttagctgaat taacacaaac
     1201 aacggtaaac cggaacaaaa gccggtgtaa acacaaagca acatattttt agtaaaaatc
     1261 tgttgccaga tagagcgttt gggttcgtga caagcggtca tatttttcct acattttgca
     1321 aaataaaaag gggaagaact gagccaatat agcataattt taaaggcgac tctaaccctt
     1381 tccatttcgt cacagataag gtttgacagc cttgcgacat atttctatta ttccttttaa
     1441 tcaaactaaa aaactggaag cagagtatga acacgcagca tagaaccgaa ttacttcaaa
     1501 atgcccttac cgaacgcatt cttatcttag acggtgcgat gggaacgatg atccaaaaat
     1561 ataaactaac cgaatctgat ttccgaggtg aacgttttaa aaacagtacc atcgatctta
     1621 aaggcaataa cgacctactc acactcaccc agccactcct gatttctgct attcacgaga
     1681 aatatttagc cgccggtgcg gatattatcg aaactaatac ctttagtgcg acgaccattg
     1741 ctcaagcaga ttatgaactt gaaagtattg cttatgagct gaatcttgtg ggggcaaaac
     1801 ttgcccgtct tgccgccgat aaattctcca tccctgaaaa acctcgtttt gttgccggta
     1861 ttcttgggcc gactaaccgt acttgttcga tttcccctga tgttaatgac cccggtttcc
     1921 gtaacattac ctttatgcaa ctggtggaag cctatgccga ggcaacccgt ggtttgattg
     1981 aaggtggggc ggatctgatt atgatcgaaa ccattttcga taccctgaat gccaaagcgg
     2041 cggtgtttgc aattgaaacg gtgtttgatg aactgggcgt gaaattgccg ataatgatct
     2101 ccggcaccat taccgatgcc tcagggcgaa cgctgtcagg gcagaccacg gaggcgtttt
     2161 ataactcgct ccgccacgcc aaaccgctga cgttcggctt gaactgtgcg ctcgggccga
     2221 aggaactccg cccttatgta gagatgatgt cgaaaatcag tgagactttt gtttcggttc
     2281 acccgaatgc cggcttgcca aacgcttttg gcgggtacga tttaggggcg gaagagatgg
     2341 cgacttacat caaagagtgg gcagaactcg gctggctgaa tattgtcggc ggctgttgtg
     2401 gcaccacgcc ggagcatatc caagcctttg ccgaagcaac caaaggcgtt gcgcctcgta
     2461 aattacctga aattccaacc gctatgcgtt tgtcgggctt agaaccgctg acgattgacg
     2521 ataattcgct gtttgtgaat gtgggcgaac gcaataacgt caccggttcg gcaaaattta
     2581 agaagctgat taaagaagaa aaatttgcgg aagcgattga aattgccatc agccaagtgg
     2641 aaaacggggc gcaggtgatt gacgtgaata tggacgaggc gttgctcgat tccaaaaaat
     2701 gtatgacccg tttcctcaac attcttgcta ccgaacctga ggcggcaaaa gtgccgatta
     2761 tgattgactc ctcgaaatgg gaggtgatcg aggcgggctt gcaaacggtg cagggcaagc
     2821 cgatagtgaa ctccatttca ctcaaagagg gcgaagagaa atttattcac caagccaaac
     2881 tctgccgccg ctatggggct gcggtggtgg tgatggcatt tgacgaagtc gggcaagcgg
     2941 ataccgaaga acgcaaggta gaaatttgta cccgagctta tcggattttg gttgatcagc
     3001 tcggcttccc accggaagac attattttcg acccgaacat ttttgcgatt gccaccggta
     3061 ttgaagaaca caataactac ggcgtggatt ttatccaagc ctgtgagcgg attaaacgag
     3121 acttgccaca cgccaaaatt tccggcgggg tgtcgaatgt ctctttctct ttccgaggca
     3181 acaatgtggt gcgtgaggct attcacgccg tgttcctcta ctatgcgatc aaagccggaa
     3241 tggatatggg gatcgtgaac gcagggcagt tggcgattta tgacgacctc gaccctgaac
     3301 tgcgagaggc gattgaagat gcggtgctga accaccgtca tgacactacc gaccgcctgt
     3361 tggaaatttc cgaaaaatat cggggcgtaa aagtagaaag tgccgatgaa agtgccgccg
     3421 agtggcgaaa tttacctgtg gcagagcgtt taaaacacgc cctcgtaaaa ggcataacga
     3481 cctacattat tgaagatacc gaagaggctc gccagcaatt tgcctctccg ctggaagtga
     3541 ttgaagggcc gctcatggcg ggtatggacg tggtgggcga cctgttcgga gacggcaaaa
     3601 tgttcctgcc gcaagtggtg aaatcggctc gggtaatgaa acagtccgtt gcctatttag
     3661 agccgtacat caacgccacc aagcagaaag gatcgagcaa cggcaaaatg gtgatcgcca
     3721 ccgtgaaagg tgatgtacac gacatcggca aaaacatcgt tagcgtggtg ttgcagtgca
     3781 ataactttga agtgatagac ttaggcgtga tggtgcctgc cgatgtaatt atcgaaactg
     3841 ctattcggga gaaagccgat ctcattgggt taagcggact aattacccca tcgctggacg
     3901 aaatggagta ttttttaggt gaaatgaacc gcttgaagct cgatattccg gtgctgattg
     3961 gcggcgcaac gacctcaaaa gagcatacgg cgatcaaact ttatccgaaa tataactacc
     4021 ccgtagtcta taccgccaac gcttcacgtt gcgtaaccgt ctgtgcgacc ttgatgaacc
     4081 ctgaggcaaa agccgaattt tgggaaaaaa ccaaagccga atacgaagaa attcagcgta
     4141 aatttgccgt tcgcaaacca cttcgcaacg gtttatctat tgaggaggct cgagccaatc
     4201 gttttgacgg atttagtggc gaatgggcag actatgtgcc gccaacgcca aaacagaccg
     4261 gcattatcga gtacaaaaat gtaccgattg ccaccctacg caaatttatc gactggtcgc
     4321 cgttctttat gctctggggc ttaatgggcg gttatcctga tgcctttgat tatccggaag
     4381 gcggcgaaga ggcacgccgt gtctataacg atgcccaacg ggtgctggac gaatttgagc
     4441 aaaacggcaa gctcaaccca agtgggatta tgggcatttt ccctgccgtg cgtgagggcg
     4501 atgatattgc aattttaaac gaagatcgca ccgcttgtat cggcaaatcc taccacctcc
     4561 gccaacaatc cgaacgaggc aaaaacagca aaagcccgta caacctctgt ttaagcgatt
     4621 ttatcgccga ccgagaaagc ggacaacaag actggttcgg aatgttcgcc gtttgtgccg
     4681 gcattgaaga acactattta gttgaaagct acaaagcggc aggcgatgac tacaacgcca
     4741 ttttgctcca agccgttggc gaccgcttgg cagaagcaat ggcggaatac ctacactttg
     4801 agctacgtac caaagtctgg ggctacaccg tggaagacga agacaacgga cgcttaatcc
     4861 gtgaagaata catcggcatc cgccctgcgc ccggctaccc aagctgcccg gaacacaccg
     4921 agaaacagat aatttgggac ttgttagagg tggaacaacg catcggcatg aaactcaccg
     4981 aaagctatgc catgtggccg gcagcaagtg tctgcggctg gtacttcacc caccctacca
     5041 gcaactactt cactctcggc aagattgatg aagatcaggt gttggattac gccaaacgca
     5101 aaggctggga cgaactcacg gcgaagaaat ggttaagtgt ggcgatgaaa caagatgtca
     5161 agtcgcaggc tgaaaacatt taatgtagtg gcgggtttta acccgcctgt tacaagcggt
     5221 tggttttgag ataaaatatg caaaatttat ctctctttta tgcaaagaag ttatcatcga
     5281 gaacaaaaca atgattgaaa aacaaaatta tcacttagta caacttttaa aagacagtaa
     5341 ttatcaatta actcaatttt ctcctgcgta tataaaaaaa ttagaggaat ctattttcct
     5401 caaatcaggg aaaaatggag atattcctca tattcaatgt cttgttcgta aaaaggatat
     5461 taagctcaca cctgaagaag cagtacgcca actctattta atgaatttgc attatgaata
     5521 tgattatcca tttgaacgaa tggaattaga atattctgtt gtattcgggc gtgagaagaa
     5581 acgagcggat attgtcatct ttgatagaga taatcctaac gctgtctata ttatggtgga
     5641 gctgaaaaag ccgaaattaa aagatggtaa agaacaatta aaatcttatt gtaatgcgac
     5701 tggagcacct attggtgtat ggacaaatgg cgaaagtatt tcttactata atcgccgtga
     5761 tcctaattat tttaaagata ttcccgatat tccgaatgct caacaaaaac tttctgattt
     5821 attacaagaa cgttggacta ttcaagatct gatagataat gacaaaattg ccaaacaacg
     5881 taaatcattg aaagatcttg ttattgaaat ggaagatgag gtactggcga atgctggagt
     5941 tgatgttttt gaagagttat ttcaacttat ttttactaaa ctctatgatg aaatggaaag
     6001 tacacgcaat aaaagtcgtt atttagagtt tcgtaactat ggtgataccg aaagtgaatt
     6061 aaaggcaaaa attcaatctc tatttgatcg tgcaaaaaat aaatgggaag gcgtatttgc
     6121 tgaaagtgcc aaaatcaacc tttccccttc gcatttggcg atttgtgtat cttcattgga
     6181 agaagtaaag ctgtttaatt ctaaccttga tgtagtggat gaagcatttg agtacttaat
     6241 taataaatcc agtaaaggag agaaaggaca atactttacg cctcgttacg tcattgatat
     6301 gtgcgtaaaa atgctcgatc caaaacctga agaaacagtc attgataccg cagcaggaag
     6361 ctgtggtttt ccagttcatt ctatttttca cgtttgggaa aaacaactta aagcgagagg
     6421 attagaacgc tcacacttat ttactgctga agaaaaattg cctgaacaaa cagattatgt
     6481 gaaagaaaaa gtctttgcga ttgatttcga tgaaaaggca gtacgtgttg ctcgtaccct
     6541 aaacttaatc gcaggagatg ggcaaaccaa cgtactacat ttaaatacct tagattatga
     6601 acgttgggaa gattttacta aggaagaaga atggaatgat gtatatggag aaggctggaa
     6661 aaaattaaga aaattacgta aaactaaaaa tgaaaatcga gatttccaat ttgatgtgct
     6721 aatggcaaat ccgccttttg ccggtgatat taaagaaact cgtatattag ctcgctatga
     6781 attaggcaaa aatagcaaag gaaaacagca atccaaagtc ggacgagata ttttatttat
     6841 tgaacgcaat ttagatttct taaaagacgg tggacgaatg gcgattgtgc taccacaagg
     6901 gcgttttaac aattccagcg ataaatatat tcgtgatttt attgcagagc gttgccgcat
     6961 tttagctgtt gtgggcttac acggaaatgt gtttaaacca cataccggca caaaaacttc
     7021 cgtactattt gtgcaaaaat ggcacgatga actttgcccg aaagtagagg attacccgat
     7081 tttctttgcc acaatgcaga aatccagcaa agataattca ggcgataaaa tctatgttaa
     7141 aaacgctgat ggcacactca aattagacaa ccatcaacac ctaattgtgg agcacgatct
     7201 atttaatcac gataatagta cccaagacgg cattgcagaa gcctttattg aatttgctaa
     7261 aaaagaaaag ctctcttttg tgggaaagga ttaagcccat ttgaccctgc taaatatcag
     7321 gcattgttag atgggctaga ggtgagcgag gtttatttga gtaaggttcg ttctgcaagt
     7381 tattcggaac gaatggatag tactttcttt ttgaaaaaat acttaaacca tccgagtttt
     7441 aattttccat tatcgctgaa tgaggtttct ctgattaaaa gtggtacaac accaacagat
     7501 cgagatgata acttaaaaga gggggttgtc ttattaaaaa cca
//
LOCUS       DANLRK010000123         7530 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.123, whole genome shotgun sequence.
ACCESSION   DANLRK010000123 DANLRK010000000
VERSION     DANLRK010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7530
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.123"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            <1..1995
                     /locus_tag="PXA33_001899"
     CDS             <1..1995
                     /locus_tag="PXA33_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDL5121207.1"
                     /translation="EIEQTRAAYDRLKRSGVASQNELRRASEATKQRVRELNAELGKT
                     SFGDKAGSFGRGLMGAGVGIAAGAAMARQPARNQMEFDRQLAMVANTAFSDRDVKGRI
                     DGKRELLDAVQKAVSVGGGSKEEALSSLDALLASGAVSTETALNLLPTLQKGAVATGA
                     SANDMAQIAISSMQQFGIKEEDIGRVLDMAVAAGQAGSFELADMASWLPQQMAAAKQA
                     GLSGIEGFERLLIANQQARVTAGTSNEAGNNLVNLLGKITAKETNERFKNIEYKDPKT
                     GKTKAIDFAKSMEHYKGKGQDSLQAFMSIMDDVVGSDKRYQELQAKLKTAKGEEQKRL
                     FNELTNLVEGTAIGEIISDRQALMALLGIKNNVQLGEKVQKDIQNSEGAVETSHAVIR
                     DTNAHKVESLKNTTEFAQMKNFEKVNGVLGTLAELLNKYANEYPNLTQFLSGATDAVK
                     IFGAALAASSVLDLLTGKKDKGGLIGDVLDKSSKASGGKGKVGKLLSVGGSFASVAGV
                     ATMLHGDQAPMSEERKAELRDPNYQARKMYEYKKEILGSRFHSFFRSNETLEQYRKDV
                     SAYEAKQKNSNTMGAVQGVLSTIPSLQELRPKVDSVTQTLSTYQADFQAFGATISAGI
                     EAGLASQSHTIANQITVELDGSVVAEKVAEYQFNFNKRMA"
     gene            1995..3287
                     /locus_tag="PXA33_001900"
     CDS             1995..3287
                     /locus_tag="PXA33_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HDL5121208.1"
                     /translation="MSGWTMPIQQASFKGVRFDVLAVDDSFERAVVEHAYPFVNGADL
                     EDMGLNTQTIRLQAVFFGSSYYTDYIRLLSVLQQKGADVLVHPIRGRLPNMLLVSANL
                     HTDAENINYVALDLTFKEATEMQPIFAFEDSLISKIDKLLLDLENLFDDGLRFWDSVG
                     VFDLKSRLLGLWGALFASFEAVRNLFDLDKKKYSIASSTSQRTYLRDGSGALNQLKEM
                     VDSGIQGRADLTTLSFKSQLLNITDAIDNTQAIVSKVARDEGFNRSKTVKLLGSDVVE
                     LGIMLDLIATTKLTKVLTELVEEREEELLIGDLELINQIVREHWLNIINKLREQQKNT
                     QKGVPSDKTKVIYETAEKLINNIRNVAHQFTALVLAVINKKPPLTVRMVMDSGTIHKV
                     AHAFYGDYERADELLRLNPHIRQPNFIERGTLLNCYWE"
     gene            3290..4417
                     /locus_tag="PXA33_001901"
     CDS             3290..4417
                     /locus_tag="PXA33_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL5121209.1"
                     /translation="MENEIVVEIDGSQHKNWKSYDIDSDFLIPADSFNFELGKSSNIE
                     VLPNYSGKTAIVKINGKTVLTGIVDTTHHTISKSGRGFSLNGRDKAAILVDCSAPITN
                     VQGLSLLDAVKKIVTPLGIKEVQLKAEKNPTLSKVDIDVGESAWEAIMRCANSAGLHC
                     WFDPKGVLIVGGADYAQPPVATLYCVKESGDSRKNNFVDATLSYDVSQSYSEVTFLAQ
                     KHGKDSDNAKHDFKWVYKNEEMELYKPKTIVLGDVENLEALMKQAKKQVSDWQLESFN
                     LTIIVPDHKTKDGTLWQAGQRVHVVCEEYDLDGIFFLMGRRFRLSRTGGTTTELRLKL
                     DGVWTPDAYQAKAQEARKRKGKKGRKKKEPVEYVGSWELGK"
     gene            4419..5081
                     /locus_tag="PXA33_001902"
     CDS             4419..5081
                     /locus_tag="PXA33_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HDL5121210.1"
                     /translation="MMRKLAKQTKEVAKGVQDSVRAAFRGVLNLVKSGSDIQQVQVSA
                     LADETIQDLELMQHFGFTSVPPAGTQAVVIPLGGKTTHGIIVATENGSFRVKNLKNGE
                     TAIYDSSGSTIILKNGRVIDIECDSFNIKCKQYSVTASGGANFNTPKLETDQQFVSQG
                     QISGNGGMVVQGGSGASFTGSLTQQGGSISTDGDVVASGKSLVTHQHNEQGDGRPTSA
                     PI"
     gene            5200..5550
                     /locus_tag="PXA33_001903"
     CDS             5200..5550
                     /locus_tag="PXA33_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015702386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HDL5121211.1"
                     /translation="MDREISPLTRDYTSENITTLQNAVYIRLTTPKGSWWADGRVGSL
                     LHLIQREKDLSRVGLLAQQYAEEALQPIIDDGRAKSITVTHEQPKDGSLNLLISVIDN
                     RGVKFEFKHPVKLV"
     gene            5563..6624
                     /locus_tag="PXA33_001904"
     CDS             5563..6624
                     /locus_tag="PXA33_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HDL5121212.1"
                     /translation="MYQIPTLEDVRREILRDIQSLEPTADIEVDSDYFVRASALASCV
                     VGLYAHQNWIVRQYFPDTADSEYLEMHAKLRNLYRKNATYASGSLKVFGTAGSVINEG
                     LQVKFGERFYVTKSSDVIGDDGSATVRVISMATGASSNVKSESKAQLTAAPAGVSSDC
                     LLLPDVTGGTEVESDAALLARLLEQIRRPPSGGNKNDFRQWAESVDGVTSAFVYPLRR
                     GEGTVDIAITSGDTVPSDEIVRATQAYIDEVRPVTAKSVYVLKPVEKRVNFNIKVQLD
                     TDTTLDGVKAEIENALENYFLSLKPADTLIISQIEAVISDLVGVVDREIVQPTRNQTI
                     DAYREMGWFRLGDVTVELM"
     gene            6624..7214
                     /locus_tag="PXA33_001905"
     CDS             6624..7214
                     /locus_tag="PXA33_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HDL5121213.1"
                     /translation="MVTINSKHGKALGGLLPPVSYDPNGRWLALSLEVEGRELDRIDA
                     RAKTLIDAVDVSTGIFIDDWERVCGLTANSSLPLETRIERVIAKLNQLGGLSIGYITQ
                     VAKELGYCIKISEPQPFIAGLSRAGDMLWHKDIMWTFFVDICNPTDDYKRFRASVSMA
                     GDALMGRYKDPILESLLEEVKPAFSRVWVRYLGDIE"
     gene            7211..>7530
                     /locus_tag="PXA33_001906"
     CDS             7211..>7530
                     /locus_tag="PXA33_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015483985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL5121214.1"
                     /translation="MKQVMNPINTPTQRFKDGNPATGEYGTIVTAEFLNNVQDSVINT
                     QQELHSVLAEAGIEANDEQVNQVAKAIKKIAGDATRDNFNALANPDGYKHIGRCKSVA
                     ELRT"
BASE COUNT         2220 a         1395 c         1905 g         2010 t
ORIGIN      
        1 gaaattgaac aaactcgtgc agcctatgac cgcttaaaac gctcaggtgt tgcctcgcaa
       61 aatgagctac gccgtgcctc agaagcgact aagcaacgag tccgagagtt aaatgctgaa
      121 ttgggtaaaa ccagttttgg ggataaggca ggaagttttg ggcgtgggtt gatgggtgca
      181 ggggttggaa ttgctgcagg tgcagcaatg gctcgccagc ctgcaagaaa tcaaatggaa
      241 tttgatagac agcttgcaat ggtagcgaat accgcctttt cagatcgtga tgtgaaaggg
      301 cgtattgatg ggaaaagaga gttacttgat gccgtacaga aagcagtaag tgtaggcggt
      361 ggctcaaaag aagaagcgtt gagctcacta gacgctttac ttgcctcagg tgctgtttct
      421 accgaaaccg ctctcaattt actcccaacg ttgcagaaag gtgcggtcgc aacaggggca
      481 agtgcaaatg atatggctca aattgctatt tcctcaatgc aacaatttgg gattaaagaa
      541 gaggatattg gtcgagtctt agatatggca gttgccgctg ggcaagccgg tagctttgag
      601 cttgcagata tggcaagttg gttgcctcaa caaatggccg ctgcaaaaca agctggctta
      661 agcggcattg agggatttga gcgactttta attgcaaacc aacaggctcg tgtcactgcc
      721 ggtacaagta acgaggctgg taataattta gtgaatctgt tgggtaagat tactgctaaa
      781 gaaactaatg agcgttttaa gaatattgaa tataaagatc cgaaaactgg caaaaccaaa
      841 gcgattgatt ttgcaaaatc tatggagcat tacaagggaa aagggcaaga ttcattgcag
      901 gcttttatgt ctattatgga tgacgttgtc gggagtgata aacgctatca agaactacaa
      961 gctaaactta aaactgctaa aggcgaagag cagaaacgct tattcaatga attaacaaac
     1021 ttagttgaag gtacggcaat tggagaaatt atttctgatc gccaagcatt aatggctcta
     1081 ttagggataa agaataatgt tcaattaggt gaaaaagtac agaaagatat tcagaatagt
     1141 gaaggagctg ttgaaacctc tcacgctgtt attcgggata ctaatgctca taaagttgaa
     1201 tcattaaaaa atacaactga atttgcacaa atgaaaaact ttgaaaaagt aaatggagtg
     1261 ttaggcacat tagctgaatt actgaataaa tacgccaatg aataccctaa cttaactcaa
     1321 tttttaagtg gtgcaacaga tgccgttaaa atttttggag cagctcttgc tgcatcaagt
     1381 gtgcttgatt tattaaccgg taaaaaagac aagggcggac taattggtga tgttttggat
     1441 aaatcatcaa aagctagtgg aggtaaaggt aaggtaggta aattgctttc ggttggtggc
     1501 tcttttgcta gtgttgcagg cgttgccaca atgctacacg gcgaccaagc cccgatgagt
     1561 gaggagcgaa aagcagagtt aagagatcct aattatcaag ctcgtaaaat gtacgagtac
     1621 aaaaaagaga ttttaggtag ccgtttccac tcgttttttc gtagtaatga aacccttgag
     1681 caatatcgga aagatgtgag tgcttatgag gctaagcaaa agaatagcaa tactatggga
     1741 gctgtacaag gggttttaag taccattccg agcttacaag agttacgccc taaagttgat
     1801 tcggttactc aaaccctatc cacctatcaa gccgactttc aggcttttgg ggcaacgatt
     1861 tcagccggta ttgaggcagg gcttgcctcg caatctcata cgattgcaaa tcaaatcacc
     1921 gtggagctgg acggttcggt ggtggctgaa aaagtggctg aatatcagtt taattttaac
     1981 aagaggatgg cgtaatgagc ggttggacaa tgcctattca acaggcgagt tttaaaggtg
     2041 tgcgttttga cgtgcttgca gtggatgata gctttgagcg agcggtagtt gaacacgcct
     2101 acccttttgt aaatggggct gatttagagg atatggggct aaacacccaa actattcggc
     2161 tgcaagcggt cttttttggc tcaagctatt acacggacta tatccgctta ctttcagttt
     2221 tgcagcaaaa aggggctgat gtattagtcc accctattcg tgggcgttta ccgaatatgt
     2281 tactcgtttc ggcgaatcta cataccgatg ccgaaaatat caattatgtt gccttagatt
     2341 tgacctttaa agaggcaacc gaaatgcagc cgatttttgc ctttgaagat tcgctgattt
     2401 cgaaaatcga caagctgcta cttgatctcg aaaacctgtt tgatgatggc ttgcgttttt
     2461 gggatagcgt tggggtgttt gatctgaaat ctcgtttgct tgggctttgg ggggcgttgt
     2521 ttgcctcgtt tgaggcggtg cgtaacctgt ttgacttgga caagaaaaaa tacagcattg
     2581 cctcatcaac ctcacaacgc acctatttaa gagacggttc gggggcattg aatcagttaa
     2641 aagaaatggt ggatagcggt attcaggggc gtgctgattt aacgacactc tcgtttaaat
     2701 ctcagttact caatattact gatgcgatag ataacactca ggcgattgtg agcaaagtgg
     2761 ctagagatga gggttttaac cgctcaaaaa cggttaagtt acttggctct gatgtggttg
     2821 agcttggcat tatgcttgac cttattgcca cgactaagct caccaaagtc ttgactgagc
     2881 tggtagaaga gcgtgaggaa gagctgctaa ttggtgattt agagttaatt aaccaaattg
     2941 tgcgtgagca ttggctgaat attatcaaca aactgcgtga gcaacagaag aacactcaaa
     3001 aaggcgtgcc gagtgataag acaaaagtta tctacgagac cgcagaaaag ctgatcaaca
     3061 atatccgtaa tgttgcccat cagtttactg cacttgtgct tgcggtaatt aataaaaaac
     3121 cgcctttgac cgtgcgaatg gtaatggata gcggcacaat ccataaagta gctcacgcct
     3181 tttatgggga ttatgagcga gcggatgagc tattacgtct taatccgcat attcgccagc
     3241 caaactttat tgagcgaggc acgctgttaa attgttattg ggagtaaaaa tggaaaatga
     3301 aattgtggtg gagattgacg gctcgcagca taaaaactgg aaaagttatg atattgacag
     3361 cgatttttta attcctgctg atagttttaa ttttgagttg ggtaaatcta gcaatataga
     3421 ggttttacct aattattcgg gcaaaacggc aatagttaaa atcaatggca aaacggtgct
     3481 cactggcatt gtggatacca cacatcacac cattagcaaa agcgggcgtg ggtttagcct
     3541 aaatggacgt gataaagccg ctattttggt ggattgctct gccccgatta ccaatgtgca
     3601 agggctctct ttgcttgatg ctgttaaaaa aattgtaacc cctcttggga ttaaagaggt
     3661 gcaactgaaa gccgagaaaa acccgacttt aagcaaggtg gatattgatg tgggtgaatc
     3721 ggcgtgggag gcgattatgc ggtgtgccaa ctcggcaggt ttgcattgtt ggtttgaccc
     3781 caaaggcgtg ttaattgtcg gcggtgcgga ttacgctcaa ccgcccgttg ccacgttata
     3841 ttgcgtgaaa gagtcggggg atagccgtaa aaataacttt gttgatgcaa cactttctta
     3901 tgatgtgtcg caaagctatt cggaggtcac ttttcttgct caaaaacacg gcaaagattc
     3961 tgataatgcc aagcacgatt ttaaatgggt gtataaaaat gaggaaatgg agctttataa
     4021 accgaaaact atcgtgctgg gcgatgtgga gaacctcgaa gccttgatga aacaggcgaa
     4081 aaaacaggta tcagattggc agttggagtc atttaacctc acgattattg tacctgacca
     4141 taaaaccaaa gacggcacgt tgtggcaagc tgggcaacgt gtgcacgtgg tatgtgagga
     4201 gtatgattta gatggtattt tctttttaat ggggcgacgt tttcgactct ctcgcacggg
     4261 tggcacaacc accgaattgc ggttaaaact ggatggcgtg tggacacctg atgcttatca
     4321 agctaaagca caagaggctc gcaagcgtaa aggcaaaaaa ggacgtaaga aaaaagagcc
     4381 tgtagaatat gtaggctcgt gggagttggg aaaataatat gatgagaaaa ttagcaaaac
     4441 agactaaaga ggtggcaaaa ggtgtgcaag atagcgtgcg tgctgctttt agaggggtat
     4501 taaatttagt caagagtggc agtgatattc agcaggtaca ggtgtccgct ttagcggacg
     4561 aaactatcca agatttagag ctaatgcagc attttggttt cacttccgtg ccacctgccg
     4621 gcacgcaagc ggttgttatc cccttaggtg gtaaaaccac gcacggtatt attgtggcga
     4681 ctgaaaacgg ctcgtttagg gttaaaaatc taaaaaatgg tgagacggct atttatgatt
     4741 caagcggctc aactatcata ttaaaaaacg ggcgagtgat tgatattgag tgcgatagct
     4801 ttaacattaa atgcaagcaa tattccgtca ccgcaagcgg tggagcaaat tttaacaccc
     4861 cgaaactaga aaccgaccag cagtttgtgt ctcaagggca gatcagcggc aacggtggaa
     4921 tggtagttca aggcggaagt ggtgcaagtt tcactggctc attaacacaa caaggaggca
     4981 gtatctcaac tgatggtgat gtggtggcat cgggtaaatc actggttaca catcaacaca
     5041 acgaacaagg cgatggcaga cctacctctg ctccaattta acttacaagc taatttacaa
     5101 gcggtctaat tttgcaaatt ttttgtagaa aaagaccgct tgccatttgt ggaagtctct
     5161 ccccgttact cctgtcttta atgtctctac catagcaata tggacagaga aatcagcccg
     5221 ctcactcggg actatacaag tgaaaatata actacactgc aaaatgccgt gtatatcaga
     5281 ctaactactc cgaaaggctc gtggtgggca gatgggcgtg taggttctct gctccatctt
     5341 attcaacgtg agaaagattt aagccgtgtg gggctattag ctcaacagta cgccgaagag
     5401 gctttgcagc cgattattga tgacggacga gccaaaagca ttacggtgac acacgagcag
     5461 ccaaaagacg gctcgttaaa tcttttaatc tccgttattg ataatcgtgg ggttaaattt
     5521 gagtttaaac accctgtaaa actggtttag gtggttttta aaatgtatca aatcccaacg
     5581 cttgaagatg ttagaagaga aattctgcga gatattcaat ctttagagcc aactgctgat
     5641 attgaggtgg attcggatta ttttgttcgt gcctccgctt tggcaagctg tgtggtgggg
     5701 ctgtatgctc accaaaactg gattgttcgt cagtatttcc cagatacggc agactccgaa
     5761 tacctcgaaa tgcacgctaa attgcgaaat ctttaccgta aaaatgcgac ctatgcgagt
     5821 ggttcgttga aagtttttgg tactgctggc tcagtaatca atgagggctt gcaagttaaa
     5881 tttggcgagc gtttttatgt aaccaaaagc tcggacgtta ttggtgatga tggctctgca
     5941 acggtgcgag tgatttcaat ggcaacaggg gcatcaagca atgttaaatc tgaaagtaaa
     6001 gcacaactga ctgctgctcc tgctggcgta tcaagcgatt gcttgttatt gcctgatgtg
     6061 acaggcggga cggaagtgga aagcgatgcg gcattgctgg ctcggttatt agagcagatt
     6121 cgccgaccac cgtcaggcgg gaataaaaat gacttccgcc aatgggcaga aagcgttgat
     6181 ggcgtaacct ctgcgtttgt ttatccatta cggcgtggcg agggtacggt agatattgca
     6241 atcacctcag gtgataccgt gccaagtgat gaaatcgtgc gagcaactca agcttatatt
     6301 gatgaagtcc gccctgttac ggctaaatcg gtttatgtac ttaagccagt tgaaaaacgg
     6361 gtcaatttta acatcaaagt gcaacttgat acggatacga cgcttgatgg tgtaaaagcg
     6421 gaaatcgaaa acgctttaga gaattatttt ttatcactca aacctgctga caccttaatt
     6481 atttcgcaaa ttgaggcagt aattagtgat ttagtgggcg tagtagatcg tgagattgta
     6541 cagccaacca gaaaccaaac gattgatgcc taccgtgaaa tgggctggtt cagattgggt
     6601 gatgtaactg tggagttgat gtgatggtaa cgattaactc taaacacggc aaggcattgg
     6661 gcggtttatt accgcctgta tcgtatgacc caaacggcag atggctagcg ttatcccttg
     6721 aagttgaggg gagagagcta gaccgcattg atgcgagagc caagacttta atcgatgccg
     6781 ttgatgtatc aacaggcatt tttattgatg attgggagcg ggtttgtggc ttaacggcga
     6841 atagctcact accgcttgaa acccgaattg agcgtgtgat tgcaaagctg aatcagttgg
     6901 gtggattgtc gataggctat atcactcaag tggccaaaga gcttggatat tgcatcaaaa
     6961 ttagtgagcc acaacccttt attgctgggc taagccgagc tggggatatg ttatggcata
     7021 aagacattat gtggacattt tttgtggata tttgcaaccc cacagatgat tacaagcgtt
     7081 ttagagcaag tgtgtcaatg gctggcgatg cgttaatggg acgatacaaa gacccaattt
     7141 tagaaagtct acttgaagag gtaaaacctg catttagccg tgtgtgggta agatatttag
     7201 gagatattga atgaaacaag tgatgaatcc gattaatacc ccaacacagc gttttaaaga
     7261 tggcaaccct gccactggtg aatatggcac gattgtgact gctgaatttt tgaataacgt
     7321 ccaagattcg gtcattaaca cccaacaaga attgcacagt gtacttgctg aagcaggaat
     7381 cgaagcaaat gacgaacaag ttaatcaggt ggctaaagca atcaagaaaa ttgcgggcga
     7441 tgctactcgt gataatttta acgcattagc caaccctgat ggttacaaac acatcggtcg
     7501 ctgcaaatcg gttgcggagt tacgtactat
//
LOCUS       DANLRK010000124         7495 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.124, whole genome shotgun sequence.
ACCESSION   DANLRK010000124 DANLRK010000000
VERSION     DANLRK010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7495
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.124"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            172..840
                     /locus_tag="PXA33_001907"
     CDS             172..840
                     /locus_tag="PXA33_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL5121215.1"
                     /translation="MAHNGSGKSTLMRLLSGDITPTSGSILVDGLPLHQLSTKEAAKH
                     IAYLPQRLPEAADFLVQELVALGRFPYQKWLQKPTEFDVQIVEQAMESTSVTQFAKQP
                     VNSLSGGEKQRVWLAMCLAQQTPYLLLDEPLAALDIVYQVEVMQLIRKLVNEQGLTVI
                     IIIHDINLASQFCDEIIALKQGELCIQGSVDEIMQEQKLLEIFGLKLHLFNHPAGLHK
                     VAVL"
     gene            837..1718
                     /locus_tag="PXA33_001908"
     CDS             837..1718
                     /locus_tag="PXA33_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL5121216.1"
                     /translation="MMFRQYKRFIFLFILQISTAFAQPNIFTPDWSIASALTEMGNPP
                     IAMGDKRIYPLWSHRPIQPNSVLDIGARYQPNRELLAQLPVELVLSNFFYAHLASVYP
                     KEIPQIDVLFDGGNTEPQQHWHTYQKATRMIGTEIKNPNAAEAYLTKTEQTLAQWGKE
                     ILNSNPDIDYYSVVQFGSPRELRIYASNSMFRVAFDLMGLKQADLGVGDRWGNKLIPV
                     SDLAKLPERTCLIVISPFSKMTQADLENSYIWQRLGFGKNRCMKVLPPVWLFGGADSI
                     SHFAEFLHNAMTQGEKE"
     gene            1718..2533
                     /locus_tag="PXA33_001909"
     CDS             1718..2533
                     /locus_tag="PXA33_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDL5121217.1"
                     /translation="MKSSFLLIGCILLSLVTAVIRLNMVWEWEWQLLLINVDLLPINQ
                     AAVKAALLPEMTTSFLAGGLLAIASSGLQHIVRNNLASDSTLAVSSGAQLALMIAVIF
                     FPAAELFSSFWIALLGSLAAIVLVLFIAKASKLNPLTLILGGLIVNILFGAIASLLTL
                     FYFDFLFGVMVWGSGSLLQDGWATSITLAITVVVAFFIFVLLSRPLTILSLDDEQARR
                     LGAPVNLLRYLVIFVCAAITALVVSKIGVIGFIGFAVQVWLILPKYDIYYFAS"
     gene            2530..3666
                     /locus_tag="PXA33_001910"
     CDS             2530..3666
                     /locus_tag="PXA33_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDL5121218.1"
                     /translation="MIAFLAGGFLLFFTNNLIGIVSRIYDTLLPVGAFTATLGAPLLI
                     FLVLRQRKAQFEASNTAYFPVKRLNSWKLPILLLCAGVILFCLLQIIAPTMNGWAISI
                     DENLMSSYRLPRSLSAIATGAMLAVSGSLLQILTRNPMASPEVLGVSSGATIAAVLSF
                     LLFPTLSSISFLLFGVLGSLTVLALIMWLSRKLQPSALMITGVAIGALVSGLMAIIQL
                     SGSPRLTAVISWLSGSTYYANPNTVWILLSVAILLISATLLFIKPLRLISLGETVSYH
                     LGLEVKKVQMIVLLLIALMSAASTLAVGPMSFIGLMTPHLAKNLGAITPEKQIPISAL
                     LGAMLMLIADWLGRYLIFPYEIGAGIIASILGGIYFLLLLKQHK"
     gene            3694..4092
                     /locus_tag="PXA33_001911"
     CDS             3694..4092
                     /locus_tag="PXA33_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HDL5121219.1"
                     /translation="MNTLSKAIKLALYSSGIGFISTAYAETVTHLDTVEVSAETPNAS
                     SEETGRYAQKKAKGSNGMALSAKDTPQSVTAVTHQQIRDQNLNTIAKALEATHGVSVS
                     LLDRGRYSFSARGFGIDKVKVDGMDLKVSK"
     gene            4127..5830
                     /locus_tag="PXA33_001912"
     CDS             4127..5830
                     /locus_tag="PXA33_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HDL5121220.1"
                     /translation="MLYDRVEVVRGATGLTTGAGDPSASVNLVRKRANSISRYTILEA
                     GIGRYADFNTMIDHSQALNNSGSVRGRFIAEHKNGNTFIKNEKERYTTLYGNIEADLT
                     DTTKLGLGVSYQKEKRDAALWGGLPAFYSDGGETNWSRSKNASPEWAYWDNESVNYFA
                     DITQKLGEKWELALKGNYRVGKHNSELFYFSGSSLNRQDGLGWSPWPGKFKNDNTQSN
                     VQLDLTGSFSAWGLEHDAAIGIQYNRAHRNSYAADNSTFDGAFDFNNWNGIYARPNWG
                     SMSEKYYQNLKEFGVYVSTRLHLTDRLSMLLGSRISSYRTEGTFYGSTQNFHANDVWT
                     PYAGLTFDINPQNTVYVSYTDIFKPQSQVDINNKLLSPVTGETYEAGWKGSYFNDNLQ
                     TQISVFETRQDNLAQSAGINPNTLLAYYTASKGAKVRGFEVEASGNVTEKLKLSAGYT
                     QWKGDDASGNPINTTQPRKQFKLFATYDMNRLVQGLTVGAGVNWQSRIYTKHATYGEY
                     GQKAYTLVNLMARYQFNDNFSAQLNIDNLFDKKYRNALSFSQYSYGEPFYTRLNLRYE
                     F"
     gene            5942..6586
                     /gene="adk"
                     /locus_tag="PXA33_001913"
     CDS             5942..6586
                     /gene="adk"
                     /locus_tag="PXA33_001913"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005721186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HDL5121221.1"
                     /translation="MKVILLGAPGAGKGTQAQFIMNKFGIPQISTGDMFRAAIKESTE
                     LGKQAKALMDEGKLVPDELTVALVKDRIAQPDCANGFLLDGFPRTIPQADALKESGVK
                     IDLVLEFDVADEVIVERMSGRRVHQPSGRTYHVVYNPPKVEGKDDVTGEDLIIRQDDK
                     PETVLERLAIYHKQTKPLIAYYTAEAEAGNTRYERLDGTKPVEEVSAELAKILS"
     gene            complement(6648..7463)
                     /locus_tag="PXA33_001914"
     CDS             complement(6648..7463)
                     /locus_tag="PXA33_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HDL5121222.1"
                     /translation="MSYKVVFSLLFSLFLSTANANWTKINDVNYTWGPFNIYHITLFT
                     ESGTYVEGTRPLMLTLQYKKPWEGRDFAVSLARTWNNLGIKLPEKELDEVITHLRKTF
                     PDIKPEDTLHYIALEDRGYFILNDKVVSDVFNKAFNDAIVAIWLDPKMDISHQLLDPS
                     YKPRAEAESIKPQVEERVAENLQEPIVNTEVSKELKADSQAVEQAPNKIDIPVADKTQ
                     ASVVNEATQPVENKDKKEPEKLEEKPKEMKIENPEVFISPIYDDILMLTKYCV"
BASE COUNT         2194 a         1478 c         1481 g         2342 t
ORIGIN      
        1 agattttcat ctactcattt tcattttgat tgatttggtg gggtaatccc acctttttta
       61 tgctcttaaa acacaacact atgattcaaa taaattccat tagctactct gttgaacaaa
      121 accgtaaact gctaagcaat atttccctca atttaagcaa ggggcgttta tatggcgcat
      181 aacggctcgg gtaaatcaac gttaatgcgt ttactaagcg gagatataac gcctaccagc
      241 ggttcaattt tagtcgatgg attaccactt catcagcttt ctaccaaaga agcggcaaaa
      301 catattgcct atttaccaca acgtttacct gaagcggcgg atttcttagt gcaggaactg
      361 gttgctttag ggcgatttcc atatcaaaaa tggctacaaa aaccaacaga atttgatgtt
      421 caaattgttg agcaagcaat ggaaagcacc tccgttactc agtttgctaa acagcctgta
      481 aatagtctat caggcggaga aaaacaacga gtttggcttg ctatgtgctt agcccaacaa
      541 acaccctatt tactgctaga tgagccattg gctgcccttg atattgttta tcaagttgaa
      601 gtaatgcagc ttattcgcaa gctagtgaat gaacaaggtc taactgtcat tatcattatt
      661 cacgatatta atcttgcctc ccaattttgt gatgaaatta ttgcgttaaa gcaaggcgaa
      721 ctctgtattc aaggttctgt tgatgaaatt atgcaagaac agaaattact tgagatcttc
      781 gggctaaaac tgcacttgtt taaccaccct gcaggcttac ataaagtggc ggtattatga
      841 tgtttaggca atacaagcgg ttcatttttc tctttatttt gcaaatttct accgcatttg
      901 ctcaacctaa tatttttaca ccagattggt ctattgcctc tgcccttact gaaatgggaa
      961 atccgcccat tgcgatgggg gataagcgaa tttatccgct atggtcgcac cgccctattc
     1021 agcctaatag cgtgttagat attggagcgc gttatcagcc taatcgcgaa ttattagcac
     1081 agcttcctgt tgaattggtg ctatcaaact ttttctacgc tcacttggct tctgtttacc
     1141 cgaaagaaat tccacaaata gacgtgctat ttgatggtgg caatactgaa cctcagcaac
     1201 attggcatac ttaccaaaaa gccactcgta tgattggaac agagattaaa aaccctaatg
     1261 cggcagaagc atatttaacg aaaactgaac aaacgttggc tcaatggggg aaagaaatcc
     1321 ttaacagtaa tccagatatt gattactact ctgttgttca atttggctct ccgagagagt
     1381 tacgcatata tgcgtctaat tcaatgttcc gtgtagcctt tgatttgatg ggcttaaaac
     1441 aagctgattt aggtgtggga gatcgctggg gaaataaact tattcctgtg agtgatttag
     1501 ctaaattacc cgaacgcacc tgtttaattg tcatctcacc tttttctaaa atgacgcaag
     1561 cagatttaga aaatagctac atttggcaac gtttaggatt tggtaaaaac cgttgtatga
     1621 aagtattacc gcctgtttgg ctctttggtg gggctgattc catctcgcac tttgctgagt
     1681 ttttacacaa tgcaatgaca caaggagaaa aagagtaatg aaatcatcat tcttattgat
     1741 tggctgtatt cttttatcct tagtaacagc tgttatccgt ttaaatatgg tctgggagtg
     1801 ggaatggcag ttactactca taaatgttga tttattgcca attaatcaag ctgcggtaaa
     1861 agcagctctt ttacctgaaa tgacaacgtc atttttagca ggcggtttat tggctatagc
     1921 cagcagtggc ttacaacata ttgtcagaaa taatttagcg tcagatagca cacttgccgt
     1981 cagtagcggg gctcaacttg cattgatgat tgcggttatc tttttcccag ccgccgagct
     2041 tttttctagt ttctggattg cgttacttgg ctctttagct gctatcgtac tggttttatt
     2101 tattgctaaa gcctctaagc tcaatcccct tacccttatt ttagggggat tgattgttaa
     2161 tattttgttt ggagctattg cctctttact gacccttttc tactttgatt ttttatttgg
     2221 cgtgatggtt tggggaagtg gctcactttt acaagatggt tgggcaactt ctattacctt
     2281 agcgataacg gtagtagttg cattttttat ctttgtctta ctctctcgtc cactcacaat
     2341 tttaagttta gatgatgaac aagctcgccg cttaggcgcg cctgttaatt tactacgcta
     2401 cttagttatt tttgtttgtg ccgccattac agcattggtt gtaagcaaaa taggtgtgat
     2461 tggctttatt ggatttgcag tgcaagtttg gctcattttg ccaaaatacg acatttatta
     2521 cttcgcctct tgattgcttt tctagcgggc ggatttttac tcttttttac caataatctc
     2581 atcggcattg ttagcagaat ttacgacact ctactacctg ttggtgcatt taccgcaaca
     2641 ctaggcgcac cattattaat tttcttggta ttacgccaac gtaaagcaca atttgaagca
     2701 agcaatacag catacttccc cgttaaacgc ctcaattcgt ggaaactgcc aatattgttg
     2761 ttatgcgctg gagttatcct attttgtcta ttacaaatca ttgctccaac aatgaatggc
     2821 tgggctatta gcattgatga aaacctgatg agttcctacc gcttgcctcg tagtctaagt
     2881 gcgatagcaa caggggcaat gcttgcggta agtggtagcc tattacaaat cttaacgaga
     2941 aacccaatgg caagccctga agtattaggt gtgagttcag gggcaactat tgctgcagta
     3001 ctgtcattct tacttttccc aacattatct agcattagct tcttgttatt tggtgtatta
     3061 gggagtttga ctgttctcgc tcttattatg tggctctctc gtaaattaca accatcagcg
     3121 ttaatgatta caggtgttgc tattggagct ttagtcagtg gcttaatggc tattattcaa
     3181 ctaagcggca gccctcgttt aacggcagtt attagttggc tgtctggctc aacctattac
     3241 gctaatccga atacagtttg gattttatta tctgttgcaa tcctacttat cagtgccact
     3301 ttactgttta tcaaaccttt acgtttgatc tcacttggcg agaccgtttc ttaccattta
     3361 gggttagagg ttaaaaaagt acaaatgatt gtattacttt taattgccct aatgagtgcc
     3421 gcctctacac ttgcggtagg gccaatgagc ttcattgggc taatgacccc tcatttagct
     3481 aaaaatttag gtgcaattac accggagaaa cagatcccta tatcggcatt attaggggca
     3541 atgctaatgc tcattgccga ttggctcggg cgttatctaa ttttccctta tgagattggg
     3601 gcaggcatta ttgcctctat tctcgggggt atctatttct tactgttact caaacaacat
     3661 aaataatttt ttattcatta cttaggagaa attatgaaca cgctatctaa agcgataaaa
     3721 cttgccttat acagttctgg tatagggttt atctcaacag cttacgctga aacagttact
     3781 catcttgata ccgtagaagt aagtgccgaa acgccaaatg cctcatcgga agaaactgga
     3841 cgttatgcac agaaaaaagc caaaggctct aatggaatgg cattgagtgc gaaagacacg
     3901 ccacaaagcg taacggctgt aacgcatcaa caaatcagag atcaaaatct taatacgatt
     3961 gccaaagcac ttgaagcaac tcacggtgtt tctgtttctt tacttgacag aggacgctat
     4021 tctttcagtg ctcgtggttt tgggattgat aaagtcaagg tagatggtat ggatctcaaa
     4081 gtaagtaaat aaccaatgga caacgggcga aaatacaggc aacagcgtgt tatacgaccg
     4141 agtagaagtt gtgcgtggtg ctacaggctt aactactggg gcaggcgatc cttctgcaag
     4201 tgttaatctt gttcgtaaac gagccaatag catttcccgt tatactattt tagaagctgg
     4261 aatcgggcgt tatgctgatt tcaatacaat gattgaccat agccaagcat tgaataattc
     4321 aggatcagta agaggccgct ttattgcaga gcataaaaac ggtaatacgt tcattaaaaa
     4381 tgaaaaagaa cgttacacca cgctttatgg caatattgaa gcagacttaa ctgacactac
     4441 caaacttggg ttaggtgtaa gctatcaaaa agaaaaacgt gatgcagcac tttggggtgg
     4501 tttacccgca ttctatagcg acggtggtga aactaactgg agcagaagca aaaatgccag
     4561 tccggaatgg gcttactggg ataatgaatc cgtaaactat tttgccgata ttacacaaaa
     4621 actaggcgaa aaatgggaac ttgcattaaa aggcaattac cgtgtaggca aacataattc
     4681 agaacttttc tatttcagcg gctcatcact taatcgccaa gatggattag gttggtcgcc
     4741 ttggccgggt aaatttaaaa atgataatac acaatctaat gtgcagcttg atcttaccgg
     4801 ttcattctct gcttggggat tagaacacga tgcggcaatc ggtattcaat acaatcgagc
     4861 acatcgtaat tcctacgctg ccgataatag cacctttgac ggagctttcg actttaataa
     4921 ttggaatggc atttatgcta gaccaaactg gggaagtatg tctgaaaaat actaccaaaa
     4981 cttaaaagag ttcggggtgt atgtttctac ccgtttacat cttacagata gactttctat
     5041 gcttctaggt tctcgtattt catcgtaccg cacagaaggc actttctatg gttcaacaca
     5101 aaacttccac gctaacgatg tttggacacc ttatgcagga ttaacctttg atattaatcc
     5161 acaaaatacc gtttatgtaa gttataccga tattttcaaa ccacaaagtc aagtggacat
     5221 caataacaaa ctactctctc ctgttacggg cgaaacctac gaagcaggtt ggaaagggag
     5281 ttatttcaat gacaacttgc aaactcaaat tagcgtattt gaaacaagac aagataatct
     5341 agctcaatct gccggtatta accctaatac attattagcc tactacacag cctctaaagg
     5401 tgcgaaagtt cgaggctttg aagtagaagc atctggaaat gtaactgaaa aattaaaact
     5461 cagtgcagga tatactcagt ggaaaggcga tgatgcttca ggtaacccaa ttaacacaac
     5521 tcaaccacgc aaacaattta aactctttgc aacttacgat atgaaccgtt tagtgcaagg
     5581 tttaacggta ggagcaggtg taaactggca aagcagaatt tataccaaac acgctactta
     5641 tggcgaatat ggtcaaaaag cttacacttt agtgaatttg atggctcgtt atcaatttaa
     5701 tgacaacttc tcagcacagc ttaacattga taacttattc gataaaaaat atcgtaacgc
     5761 cctcagcttt agccaatata gctacggtga gccattctat acacgcttaa atttacgcta
     5821 tgaattctaa ttccgtataa caagcggtca aaaaattaaa aaaatttgca attttaggca
     5881 gtaatcggta aggttactgc ctaagtgatt ttattatttt tagtttaatt gaggttttca
     5941 aatgaaagtg attttattag gtgcgccggg tgcgggcaaa ggtacgcaag ctcagtttat
     6001 tatgaacaaa ttcggtattc cgcaaatttc aaccggcgat atgttccgtg cagcgattaa
     6061 agaaagcact gagcttggta aacaagcaaa agcattgatg gacgaaggca agttagtgcc
     6121 ggatgaatta acggtcgcct tagtaaaaga tcgtattgct caaccggatt gtgctaatgg
     6181 cttcttatta gacggtttcc cacgcaccat tccgcaagct gatgcgttaa aagagtcagg
     6241 tgtaaaaatt gatttagtgt tagagtttga tgtagctgat gaagtcattg ttgagcgtat
     6301 gagtggtcgt cgtgttcacc aaccttcagg gcgtacttac cacgtggtgt acaatccacc
     6361 gaaagtggaa ggcaaagatg atgtgactgg tgaggattta attatccgcc aagatgacaa
     6421 acctgaaacg gtattagagc gtttagcgat ttaccacaaa caaaccaaac cgttaatcgc
     6481 ctactacacc gcagaagcag aagccggcaa cactcgttat gagcgtttag atggaacaaa
     6541 accagtggaa gaagtaagtg ctgagttagc gaaaatttta tcttaaatgg attattaaaa
     6601 tgaacaagcg gtaatatttt tcgagaatat taccgcttgt tctttgctta aacacaatat
     6661 ttggtgagca ttaagatatc atcataaatc ggtgaaataa acacttccgg attttcaatc
     6721 ttcatctcct ttggtttttc ttctagcttt tctggctctt tcttatcttt attctctacc
     6781 ggttgagtcg cttcatttac cacactagct tgagttttat ctgctacagg tatatctatt
     6841 ttgtttggtg cttgctctac tgcttgagaa tccgctttca gctctttaga aacttcagta
     6901 tttacgatag gttcttgtaa gttttccgct actctttcct ctacttgtgg cttaatactt
     6961 tctgcctctg ctctcggttt ataagatgga tcaagtaatt gatggctaat atccattttc
     7021 ggatcaagcc aaattgctac aatagcatca ttgaaagctt tattaaacac atcagagact
     7081 actttatcat ttaaaataaa ataacctcta tcttctaatg caatatagtg caatgtatct
     7141 tcaggcttaa tatctgggaa agtttttctc aggtgggtaa taacctcatc taactctttt
     7201 tcaggtagct taatgcctaa attattccaa gttcttgcta aagaaaccgc aaaatcacgc
     7261 ccctcccacg gttttttata ttgtagagta agcattaaag gacgtgtacc ttctacataa
     7321 gtaccgctct cagtaaaaag cgtaatatgg taaatattaa atggccccca cgtataattc
     7381 acatcattaa tttttgtcca atttgcattt gctgttgata aaaatagaga aaacaacagc
     7441 gaaaagacca ctttatagct cattattttt gtgtccttaa atctaaaatt cctac
//
LOCUS       DANLRK010000125         7487 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.125, whole genome shotgun sequence.
ACCESSION   DANLRK010000125 DANLRK010000000
VERSION     DANLRK010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7487
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.125"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(78..1661)
                     /locus_tag="PXA33_001915"
     CDS             complement(78..1661)
                     /locus_tag="PXA33_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL5121223.1"
                     /translation="MKKQFEHNESRRGFMKLIAGVGAGMAFSGTLGTFTPKAFAAPAA
                     GSTIEAGIAYPISTGFDPLTSSGASSMAANLHIFEGLVDLHPATRKPYLALAAAEPEK
                     IDDVTYRITLRDGAKFHNGNPVTTEDVVYSFERVLDPAKASLFAQFIPFIDTVKKVDD
                     KVVEFKLKYPFALFKERLTIVKIVPKAVVEAGQAAFDANPVGTGPYKFVSATKDDRIV
                     FEAFADYNGGYPAQVEKMTWFLLSDDAARVTAQESGRVQAIESVPYLDAERLKRKGTV
                     ESVQSFGLLFLMFNCEKAPFNNPKVRQALHYGLDTQKLIDVVFLGNAKAATSYVQDTH
                     PDYVKANSQYDFDKAKAEALLAEAGVTELKFELLATDHSWVKECAPLILESWNSLKGV
                     KVSLKHLQSGALYGTHVDKGAYEVVIAPGDPSVFGNDLDLLLSWWYRGDVWPKRRFRW
                     SETAEYAEVQKLLDEAAKNPAASKEAWAKAINIIAEQVPLYPIIHRKLPTAWNDKALT
                     GFQPLPTTGMSFIGVGRAK"
     gene            1883..3040
                     /gene="hemW"
                     /locus_tag="PXA33_001916"
     CDS             1883..3040
                     /gene="hemW"
                     /locus_tag="PXA33_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HDL5121224.1"
                     /translation="MNLILPPLSLYIHIPWCVQKCPYCDFNSHAQKGLIPEQEYIEHL
                     LADLSQDLTAYRVAIGDRKLYSIFIGGGTPSLFSAEGIAYLLIEVEKRIAFEPNIEIT
                     LEANPGTAEAERFLGYAQGGVTRISMGIQSFEPEKLLKLGRIHDANEAKQAIKFAQNS
                     AKSGLQSFNIDLMHGLPNQSVKQALADLETGISLNPPHLSWYQLTIEPNTMFYYRKPT
                     LPDDDELWDIFEQGHQLLTNAGYEQYETSAYAKKGYQCRHNLNYWRFGDYLAIGCGAH
                     GKISYPTGEIYRFSKIKHPKGYMRGEYRYSQDLIELDDRPFEFFMNRFRLLEATPKQE
                     FEWFTGLDREVVRPTIDWALNQNYISETATHWQITQHGKLFLNELLEGFLE"
     gene            3183..3665
                     /locus_tag="PXA33_001917"
     CDS             3183..3665
                     /locus_tag="PXA33_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HDL5121225.1"
                     /translation="MIDKQIRNSTAEFLIFTQQNGAENITVRYEDNEIWLTQKLIAQL
                     FDCSTDNVSLHLKNIFNSRELDPNSVTEDFSVTAADVKNYRTKHYNFDAVISIGYRVN
                     SVRATQFRQWATKVLRNFAQKGYVIDRQRMENGTFLNEDYFEQLLAEIREIWLSERRS
                     "
     gene            3634..3831
                     /locus_tag="PXA33_001918"
     CDS             3634..3831
                     /locus_tag="PXA33_001918"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121226.1"
                     /translation="MKFGYLNGVLEGKGKMRFDEAKLHAESEFEKYRIVQDRLFESDF
                     DKVLKEMLRSENQYRPSFSTT"
     gene            3918..4385
                     /gene="bcp"
                     /locus_tag="PXA33_001919"
     CDS             3918..4385
                     /gene="bcp"
                     /locus_tag="PXA33_001919"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HDL5121227.1"
                     /translation="MNILQAGDKAPKFTLQNQADEPVSLSQFAGKKVLVYFYPKALTP
                     GCTTQACGLRDSKSELDELNVVVLGISTDLPKKLAQFVEKKALNFTLLSDPDHQVAEA
                     FGVWGEKKFMGKTYDGIHRISFLIDEQGGVEQVFDKFKTGEHHQMIVDYLRGR"
     gene            4385..4933
                     /gene="gmhB"
                     /locus_tag="PXA33_001920"
     CDS             4385..4933
                     /gene="gmhB"
                     /locus_tag="PXA33_001920"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HDL5121228.1"
                     /translation="MGKKAIFLDRDGTINIDHGYVHQIDDFQFIDGVGKALKQLQDKG
                     YLLVLVTNQSGIARGYFSEAQFMQLTEWMDWSLDEDYGVVLDGIYYCPHHPEGKGEYR
                     KDCDCRKPKAGMFTQAIQDLNIDPSQSYMVGDKLEDILAAEAAGVKTKVLVRTDKAVT
                     EEGQAKADLVLDSLVDLVKYIK"
     gene            4938..5777
                     /gene="queF"
                     /locus_tag="PXA33_001921"
     CDS             4938..5777
                     /gene="queF"
                     /locus_tag="PXA33_001921"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HDL5121229.1"
                     /translation="MNYNDKSLSALKLGQKTEYKSEYDASLLQPVPRKLNRDSLGITQ
                     EQPFNRGADVWTCYELSWLNQNGLPQVAIADVAIDFKSENLIESKSFKLYLNSFNQTK
                     FSSIEEVGKTIQADLARCASGQVSVKIYQLSHYTQQPIVDFAGDCIDEQDIQIDSYAF
                     STHYLENIAEDELVEETLVSHLLKSNCLITSQPDWGSVQIRYKGKKLNREKLLRYLVS
                     FREHNEFHEQCVERIFCDLMQFAEPEKLTVYARYTRRGGLDINPFRSNFEEVPENLRM
                     ARQ"
     gene            5969..7102
                     /locus_tag="PXA33_001922"
     CDS             5969..7102
                     /locus_tag="PXA33_001922"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HDL5121230.1"
                     /translation="MLIFMMNEYSPNYYIGVMSGTSLDGVDIALLDFAKNPPKMTACD
                     FFPMPEELRADISALLKTGETNLQKLGEIDHRLGLLYAETINAFLAKNQLKAEDIQAI
                     GCHGQTVWHSPDSEYPFTTQIGDMNLVAEQTGITTIADFRRKDMAVGGQGAPLVPAFH
                     QAIFSDPNRLTVVLNIGGISNISVLNPNSPTVGYDVGAGNTLMDSWIEQHQGKRYDKN
                     GEWARTGKLNTELLATLLDESFFAKAPPKSTGRELFNLTWLAKKLENLTACQPQDVQR
                     TLAEFTAQSIANELLRFESSLPRLLLVCGGGSRNPLLMERISALLPEWEVTTTTEFGL
                     DIDYVEAAAFAWLAYQRIHNLPSNMPSVTGAKKAVSLGVIYPA"
     gene            complement(7099..>7487)
                     /locus_tag="PXA33_001923"
     CDS             complement(7099..>7487)
                     /locus_tag="PXA33_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL5121231.1"
                     /translation="YIFTNLLDKPELVSVSFFVNNLIAVLLQSDFSRKLNLTVHKLIY
                     IAPILIILQIMPWFYALQWLSTITAFLYLVIFATITVYANTALSNMLVKLDNGKNQGL
                     MFGSSKLILAITTLGVMNVLPYIFLI"
BASE COUNT         2281 a         1428 c         1615 g         2163 t
ORIGIN      
        1 atcattttga tttttattta tgcaagcggt aaattttttg taaaatatta ccgcttgcat
       61 ttagctagcc gttacgacta tttagcacga ccaacaccga taaatgacat acctgttgtt
      121 ggtaatggtt ggaaacctgt taatgcctta tcattccacg cagttggcaa tttacggtga
      181 ataattgggt aaagaggcac ttgttcagcg ataatattga ttgctttcgc ccaggcttct
      241 ttagaagcag caggattttt ggccgcttca tcaagcagtt tttgtacttc tgcatattca
      301 gctgtttcag accagcggaa acgacgtttc ggccatacat caccacgata ccaccagctt
      361 aataataggt ctaagtcatt accgaaaact gatgggtcgc caggtgcaat cacaacttcg
      421 tatgcacctt tatcaacgtg tgtgccatat aatgcacctg attgtaagtg tttgagtgaa
      481 actttcacgc cttttaagct gttccacgat tcaagaatta atggcgcaca ttctttcacc
      541 caagagtgat cggttgccaa caattcaaat tttaactctg tcacacccgc ttctgctaat
      601 aacgcttcgg ctttcgcttt atcgaaatca tattgcgaat ttgctttcac ataatccggg
      661 tgagtatctt gcacgtaaga tgtagcagct tttgcattgc ctaagaacac cacatcaatt
      721 aatttttggg tatctaagcc ataatgtaac gcttggcgaa ctttcgggtt gttaaacggt
      781 gctttttcac agttaaacat taagaacagt aagccgaatg attgtactga ctcaacggta
      841 cctttacgtt ttaagcgttc tgcatctaag taaggcacag actcaattgc ttgaacacgg
      901 ccagattctt gagcggttac acgagcggca tcatcagaaa gtaagaacca agtcattttc
      961 tcgacttgtg ccgggtagcc accgttataa tctgcaaatg cttcgaaaac aatacgatca
     1021 tctttagttg cggatacaaa tttatatggg ccagtcccca ctggattagc atcaaatgca
     1081 gcttgaccgg cttctaccac tgcttttggt acgattttaa caatggttag gcgctctttg
     1141 aataacgcaa atggatattt caatttgaat tcaaccactt tgtcatcaac ttttttcaca
     1201 gtgtcaataa atggaatgaa ttgtgcgaaa agtgatgctt tagctgggtc taatacacgt
     1261 tcaaatgagt aaactacgtc ttcagtggta accggattac cattgtggaa tttagccccg
     1321 tcacgtaaag taattcgata ggttacatca tcaatttttt caggctctgc tgctgccaat
     1381 gctaaatatg gtttgcgagt ggctggatgt aagtccacga ggccttcaaa aatatggagg
     1441 tttgccgcca ttgaggatgc accgcttgag gtaagcgggt caaatccggt tgatatggga
     1501 taagctatac cggcctcaat cgttgagccg gcggcagggg ctgcaaatgc tttaggtgta
     1561 aatgtaccta aagtgccact aaatgccata ccggcaccga cacctgcaat tagtttcata
     1621 aaaccacggc gggattcgtt gtgttcaaac tgttttttca tcttaaagct cctttaagtt
     1681 tttatgatag gggcggtatt atttgctgaa gtgaatttat caaaaaatgc acttttttga
     1741 attgaattta tcatatcatt aatgtcaaag tggtaaagca agaattattg aatttgtgat
     1801 ctagctcacg aaaatctttg cttttcactt cacgtgcttt tcgttattat tcgccaattt
     1861 aatcataaat gaaaaattga cagtgaacct gattttaccg cctttaagcc tttatatcca
     1921 tatcccttgg tgcgtgcaaa aatgccctta ttgcgatttt aactcgcacg ctcaaaaagg
     1981 attaattccc gagcaagaat atatcgaaca tttacttgca gatttatccc aagatttgac
     2041 cgcttaccga gtggcaatag gtgatcgtaa attatattct atttttattg gcggcggcac
     2101 cccgagctta ttttcagcgg aagggatagc ttatttatta attgaagtag aaaaacggat
     2161 tgcattcgag ccaaatatcg aaattacact ggaagcgaac ccgggaaccg cagaggcaga
     2221 gcgattttta ggttacgcac aaggtggcgt aacccgcatt tcaatgggaa tccaaagttt
     2281 tgagccggaa aaattattga aattaggacg gattcacgat gcaaatgaag caaaacaagc
     2341 gattaaattt gcccaaaatt ctgcaaaatc gggcttgcaa agtttcaaca ttgatttaat
     2401 gcacggctta ccaaaccaat cggttaaaca agccttggcg gatttagaaa cagggatttc
     2461 actcaatcca ccgcatttat cgtggtatca gctcacgatt gagccgaata cgatgttcta
     2521 ttatcgtaag ccaaccttgc ctgatgatga cgagttatgg gatattttcg agcaagggca
     2581 tcaactttta acgaatgccg gctatgagca gtatgaaacc tcggcttatg ccaaaaaagg
     2641 ctaccaatgc agacacaatt taaattattg gcgatttggg gattatttgg cgattggttg
     2701 tggcgctcac ggcaaaattt catatccgac cggggaaatt tatcgcttta gcaaaatcaa
     2761 acacccgaaa ggttatatgc gaggtgaata tcgctacagc caagatttga ttgaattaga
     2821 cgaccgcccg tttgagtttt ttatgaaccg ctttagatta cttgaagcga caccaaaaca
     2881 agaatttgaa tggtttacag ggttagacag ggaagttgtt cgcccgacaa tcgattgggc
     2941 gttaaaccaa aactatattt ccgaaaccgc aacccattgg caaattaccc aacacggtaa
     3001 attgttttta aatgaattac tggaaggatt tttggaataa tttgcaaaaa aacgactaaa
     3061 aaaagaccgc attgtaatgc taaattacag gcgttttttg tgatctgcgt cgaaaaatcc
     3121 tcatttttta ctagacaaaa aaacagtatt tagtagcata gccttcacaa taaggagagc
     3181 ttatgatcga taaacaaatc cgtaatagta ccgccgaatt tttgattttt actcaacaaa
     3241 atggggcaga aaatattacc gttcgctatg aagataacga aatttggctt acgcaaaaat
     3301 taattgctca gctttttgat tgctcaacag ataatgtttc tttgcattta aaaaacattt
     3361 ttaatagtag ggaactcgat ccaaattcag ttaccgagga tttctcggta actgccgcag
     3421 atgtcaaaaa ttatcgtacc aaacactaca actttgatgc cgttatttcg ataggctata
     3481 gggtaaactc ggtgcgggcg acacaatttc gccaatgggc aactaaagta ttacggaatt
     3541 tcgcccaaaa aggctatgtg attgaccgcc aaagaatgga aaatggtaca tttttaaatg
     3601 aagattattt tgagcagttg ctggcagaaa ttcgtgaaat ttggttatct gaacggcgtt
     3661 cttgagggca aaggcaaaat gcgttttgat gaagcgaaat tgcacgcaga aagcgaattt
     3721 gagaaatacc gcattgttca agacagatta tttgaaagcg attttgacaa ggtattgaaa
     3781 gaaatgttgc gttcggaaaa tcaataccgt ccatcatttt caacaacata aaacaaccgc
     3841 attttgcaaa ttttttgcca agtttgaccg cttgccgtgt ataatgcaag cggttatttt
     3901 ttatggaaaa attacaaatg aatatcttac aagcaggcga taaagcccct aaatttaccc
     3961 ttcagaatca ggctgacgag ccggtttctc tctctcaatt tgccggcaaa aaagtactgg
     4021 tttattttta tccgaaagct cttacacccg gttgcactac gcaagcgtgc ggactgcgtg
     4081 acagcaaatc agaacttgat gagctcaatg tggttgttct cggaattagc accgatttac
     4141 cgaaaaaatt agctcaattt gtagagaaaa aagcacttaa ttttacctta ttatctgacc
     4201 cggatcacca agttgcagaa gcctttggtg tatggggcga aaagaaattt atgggaaaaa
     4261 cctatgacgg cattcatcgc atcagttttt taattgatga gcaggggggg gttgagcaag
     4321 tgtttgataa atttaaaacc ggcgaacatc accaaatgat cgtggattat ctacggggtc
     4381 gctaatggga aaaaaagcca tttttttaga ccgtgacggc acaattaata tcgatcacgg
     4441 ctatgtgcat caaattgatg attttcaatt tattgacggt gtcggcaagg cgttaaaaca
     4501 gctacaagac aaaggttatt tattggtgct ggtaaccaat caatctggca ttgcccgagg
     4561 ctattttagc gaagcacaat ttatgcaact aaccgaatgg atggactggt cgctcgatga
     4621 agattatggc gtggtactag acggtattta ctactgccca catcaccccg aaggcaaagg
     4681 cgaataccgt aaagattgtg attgccgtaa gcctaaagca ggtatgttta ctcaagcgat
     4741 tcaagattta aacatcgacc caagccaatc ttatatggtg ggcgataaat tagaagatat
     4801 ccttgccgca gaagccgccg gcgtgaaaac caaagtgctg gttcgcactg acaaagcagt
     4861 gacggaagag ggtcaggcta aagccgattt ggtgttagat agtttagttg atttagtaaa
     4921 atatattaaa taaagcaatg aattataacg ataaatccct ttccgccctg aaattagggc
     4981 aaaaaaccga gtacaaaagc gaatatgatg cctcgctttt gcaacctgtt cctcgtaaat
     5041 taaaccgtga tagtttaggc attacacaag agcagccttt taaccgaggg gctgatgtgt
     5101 ggacttgcta tgagctatct tggttgaacc aaaatgggct accgcaagta gcgattgcag
     5161 atgtcgccat tgattttaag agcgaaaact taattgaatc gaaaagtttc aagctctatt
     5221 tgaacagttt taaccaaacc aaatttagct caatagaaga agttgggaaa acgattcaag
     5281 cggatctggc tcgctgtgca agcggtcagg tttcggtaaa aatttaccaa ttatcacact
     5341 acacgcaaca gccgatagtt gattttgcag gcgattgcat tgatgagcaa gatattcaaa
     5401 tcgacagtta tgcgttttcg actcattatt tagagaacat tgcagaagat gagttggtgg
     5461 aagaaacctt agtcagccat ttgctcaaat ctaactgctt aattacgtca cagcctgact
     5521 ggggaagtgt gcaaattcgc tacaaaggca aaaagctaaa ccgtgaaaaa ctgttacgtt
     5581 atttagtgtc gttccgtgag cataatgagt tccacgaaca atgtgtggaa cgcattttct
     5641 gtgatttaat gcaatttgcc gagccggaaa aattaacagt ttacgcacgc tacaccagac
     5701 gaggcgggct agacatcaat ccgtttagat cgaattttga ggaagtgccg gaaaatctaa
     5761 gaatggcacg ccaatgattt tgttgttttt tgttcccgtc tgaaccgcct gagcgtgttt
     5821 gaattggtag gtaaatttac tctgagcgga gcgaatgtaa atttgccgtt aagccaattc
     5881 aaactaagcg aggatagcgg tgaagctcgg gttgcctttc ttttggttac ttttctttgg
     5941 caaagcaaag aaaagtaaca aatagcagat gttgattttt atgatgaatg aatatagccc
     6001 aaattactat ataggcgtaa tgtccggcac gagccttgat ggggtggata ttgctttgct
     6061 ggattttgca aaaaatccac cgaaaatgac cgcttgtgat ttttttccaa tgccggagga
     6121 attacgggcg gatatttctg cgttacttaa aaccggcgaa accaatctcc aaaaactggg
     6181 ggaaattgat caccgtttag gcttgcttta tgcagaaacg atcaatgcgt ttttagcgaa
     6241 aaatcagcta aaagcagaag atattcaagc cataggttgt cacgggcaga cggtgtggca
     6301 ttcaccggat tctgagtatc ccttcaccac ccaaattggc gatatgaatt tggtggcaga
     6361 gcaaaccggt attaccacta tcgctgattt tcggcgtaaa gatatggcag taggtgggca
     6421 aggggctccg cttgtgccgg catttcatca agctattttt agcgatccaa atcgtctaac
     6481 agtggtgctg aatattggcg gcatcagcaa tatttcggtg ttaaatccca attcccctac
     6541 agttggttat gatgtaggag ccggaaatac tttgatggat agttggatcg aacaacatca
     6601 aggcaaaaga tacgataaaa acggcgaatg ggcgagaacg ggcaagctca atactgaact
     6661 gttagcgacg ttattagacg agtcgttttt tgctaaagcc ccgcctaaaa gcactgggcg
     6721 ggagctgttt aatttaacgt ggcttgcaaa aaaattggaa aatttgaccg cttgtcagcc
     6781 acaagatgtg caacgcacgc tggcagaatt tactgctcaa agtattgcca atgagttatt
     6841 acgctttgaa agctctctgc ctcgcttgct tttagtttgt ggtggtgggt caagaaatcc
     6901 gttattaatg gaacggattt ctgctttact tcccgagtgg gaggttacaa ccactacaga
     6961 atttggttta gatattgatt atgtagaggc tgccgctttt gcttggttag cttaccagcg
     7021 aatccataat ttacccagta atatgcctag cgtgacagga gcaaaaaaag ccgtgagttt
     7081 gggcgtgatt taccctgctt agattaagaa aatgtaaggt aatacattca ttactccgag
     7141 agtggtaata gctaaaatta atttagagct tccaaacatt aagccttggt ttttaccatt
     7201 gtctaatttc actagcatat tagatagtgc cgtattcgca tacacggtaa tagtggcaaa
     7261 aatcactaaa tagagaaatg cagtgatggt tgaaagccat tggagtgcat aaaaccaagg
     7321 cattatttga agtataatca gtattggtgc aatataaata agtttatgta ctgtcagatt
     7381 taattttcgt gaaaaatcag attgcaataa cactgcaatt aaattattca caaagaatga
     7441 gacagagact aattctggtt tatccagaag attggtgaat atatagg
//
LOCUS       DANLRK010000126         7399 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.126, whole genome shotgun sequence.
ACCESSION   DANLRK010000126 DANLRK010000000
VERSION     DANLRK010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7399
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.126"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            <1..2551
                     /gene="topA"
                     /locus_tag="PXA33_001924"
     CDS             <1..2551
                     /gene="topA"
                     /locus_tag="PXA33_001924"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HDL5121232.1"
                     /translation="YLGSNYVVKSSVGHIRDLPKSGSDEKTEKAKPISTKGLTAAEKA
                     KIKAEKERVALVKRMGIDPYNGWKANYQILPDKEKVVSELKSLAKKADHIYLATDLDR
                     EGEAIAWHLREIIGGDDSRFSRVVFNEITKDAIKKAFDDPQPLNIDRVNSQQTRRFLD
                     RVVGFMVSPLLWKKVARGLSAGRVQSVAVKLLVEREREIKAFQSEEFWTILANTQINR
                     KKLPLELTTYKGKKFSASNEAEANVAVRALEQSDFIVSNIEAKETSSRAAAPFITSSL
                     QRAASTRLGFGVKKTMMLAQRLYEAGYITYMRTDSTNLSREALAMARGYIQDNFGANY
                     LPEKANVYRSKDNAQEAHEAIRPSDVNVKMADLKGMDKDAERLYDLIWRRFLACQMLP
                     ARYDSTAVTVSAGDYELKANGRVLRFDGWTKVLPVQGKNPEDQELPAVELHQKLTLDE
                     IVPSQHFTKPPARFNEASLVAELEERKIGRPSTYASIISTIQERGYVRTENKRFYVEK
                     IGEVVTDRLNESFKDLMNYDFTANMEEVLDQIAEGSKNWKEELNTFFKHFSEELSQAE
                     LDELEGGMKPNSLVPTDIDCPTCGRKMAIRTASTGVFLGCTGYALPPKERCKTTMNLI
                     PEAEFFNVLDSDSETKALMARKRCPKCDSAMDSYVIDAERKLHVCGNNPNCDGHIIEQ
                     GSFKIKGYDGPVVECDKCGSDMHLKLGRFGKYMGCTSCDNTRKILKNGEVAPPKEDPI
                     AFPELKCEKADAYFVLRDGASGVFMSAHNFPKVRESRAPKVVELAQYRERLPEKLQYL
                     ADAPQQDPEGNEAIIRFSRKEKRQYVTSEKNGKATKWIVDYLDGKWVERKK"
     gene            complement(2601..3539)
                     /gene="yvcK"
                     /locus_tag="PXA33_001925"
     CDS             complement(2601..3539)
                     /gene="yvcK"
                     /locus_tag="PXA33_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HDL5121233.1"
                     /translation="MENTDIIPFKPTRHHYLNQLNAVVALGGGHGLGRLLSALSFLKE
                     RLTGIVATTDNGGSTGRIRSQQGVIAWGDLRNCLNQIIVKPTIASRLFEYRFAGTGEL
                     AGHNLGNLILTALENMQIRPLEGINLVRELLHVRSQLIPMSEIPVHLIAQLHSGESVF
                     GEVQIDSLAETPQHLALDTMVVATPEAISAIQSAELILLGPGSFFTSIMPNLLVAGIA
                     NSIAQSKARVVFIDNIGQEHGPAGQLSLNQRIEWMENCIGQARINAVITTDDAGSELP
                     EHIQILRKNLSANDVYYRHDRNKLSKAIDELLAQNI"
     gene            complement(3553..4629)
                     /locus_tag="PXA33_001926"
     CDS             complement(3553..4629)
                     /locus_tag="PXA33_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL5121234.1"
                     /translation="MFQMLQDWYRKKFTDPQIVVLFSILLIGFGIIYFFSDLLMPLLV
                     ALVFAYLLEWPIRFLSSKLKLPRTLSVILVLGGFIALLSFLGVVLLPSLWNQAVTFIQ
                     DLPSMFNLLNAWLQALPEHYPELVDYATLDSIVNTAKSNILSMGESLLTLSINSIISL
                     VGLGIYTFLVPLMVFFLLKDKIVLMRSFSKVLPQNRRLATRVWFEMQQQIANYIRGKF
                     LEILIVGVVTYIIFLFFDLRYPLLLSVAVGISVLVPYIGAVLVSIPVMLIALFQFGLS
                     PDFYYLMLAFIISQLLDGNLLVPFLFSEAVNLHPLTIIIAVLIFGGLWGFWGVFFAIP
                     LATLVKAVLQALPSAQSDAIILEK"
     gene            complement(4660..5415)
                     /locus_tag="PXA33_001927"
     CDS             complement(4660..5415)
                     /locus_tag="PXA33_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL5121235.1"
                     /translation="MKKEEVGHHFLARLGKTRLRPGGKLATDWLIANGDFDKNKKVLE
                     VACNMGTTAIQLAQQFECQIVGIDLDEDALEKARKNIKENHVEHLIQVQRANATKLPF
                     DDESFDIVINEAMLTMLPLEAKEKAIREYFRVLKPNGFLLTHDIVIQDKDSDTLLAEL
                     RDAINITVSPLAKADWKSLFQRCGFRNVDTFSGEMSLLSPKGLIHDEGVLGAMKIIGN
                     ALKPENRETFNKMYKLFNDPDKKLGFIAVCSQK"
     gene            5567..5776
                     /locus_tag="PXA33_001928"
     CDS             5567..5776
                     /locus_tag="PXA33_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome alternative rescue factor ArfA"
                     /protein_id="HDL5121236.1"
                     /translation="MSKQKNDYLHQRGQIKESAVKALVTDPLFRQRVERNRKGKGSYR
                     RQEKHKKTIYGQKETPFKNIRLESF"
     gene            complement(5825..6547)
                     /gene="bioD"
                     /locus_tag="PXA33_001929"
     CDS             complement(5825..6547)
                     /gene="bioD"
                     /locus_tag="PXA33_001929"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HDL5121237.1"
                     /translation="MPSLFITGTDTNVGKTVVTRAIIQTLTKHSFPVIGYKPIACGGD
                     DSLPTEPTQYDYATEDNSDVLTILDSCPTNVSYRDINSYTFIHSSTPVFAALDAVHHI
                     QLEKLNTDLKRLENAYPNVVVEGTYGWLTPINKDYYFADWVMQNNMPVVLVVGIKEGC
                     VNHALLTANEILQRGVKLVSWVANRVNPGLRHYHELIELLTQKINAPLLGQIPYMGHP
                     EHKELSSYIQNPEPLLAYFSKP"
     gene            complement(6595..>7399)
                     /locus_tag="PXA33_001930"
     CDS             complement(6595..>7399)
                     /locus_tag="PXA33_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL5121238.1"
                     /translation="QLYCVNTSQLLAVSISVIGKINAEKDSITQLGNKNIFCNITSKL
                     SPLFHCPILLSEHFQLWLLAESTVGSLINQDNAIFLQLDDTVNLSVLLSGSLLSKQSK
                     MNVDNMLMPRFSHLSDEIFPELDEIHRYKLVNQVTFPAIVRLIDRYLPNNLKSQEQKI
                     SWLCQKIDENQPLALAILSHITDNLAYALMNLVNIFSSEKIMLNSPLIKIKEPLFTQI
                     SEKLHKNLLQTNLKIDLVTSQYDWNSPLIACSAIKQGIYDGNLIKDRIN"
BASE COUNT         2352 a         1356 c         1533 g         2158 t
ORIGIN      
        1 atatcttggt agtaattatg tggtgaaatc gagtgtgggg catattcgtg atttaccgaa
       61 aagtggtagt gatgaaaaaa cggaaaaagc gaaaccgatt tcaaccaaag ggctgaccgc
      121 tgccgaaaaa gctaaaatca aagcagaaaa agagcgtgtt gcattggtta aacgtatggg
      181 tatcgacccc tataatggct ggaaagctaa ctatcagatt ttgccggata aagagaaagt
      241 ggtttccgag ctaaaatcgt tggcaaagaa agcagatcat atctatctgg caaccgactt
      301 ggatagagaa ggggaggcga ttgcgtggca tttgcgtgaa atcattggtg gtgatgacag
      361 ccgttttagc cgagtggtat ttaatgaaat caccaaagat gcgatcaaaa aggcttttga
      421 cgatccgcaa ccgctcaata ttgaccgtgt aaattcccag caaacccgcc gctttttaga
      481 ccgtgtagtg ggctttatgg tgtcgccatt attatggaaa aaagtagctc gtggtttatc
      541 ggcagggcgt gtgcaatcgg ttgccgtaaa attactggtt gagcgtgagc gtgaaatcaa
      601 ggcgttccaa agcgaggagt tttggactat tcttgccaat acgcaaatta atcgtaaaaa
      661 attaccgctt gaattaacta cttataaagg taaaaaattc tcggcaagca atgaggctga
      721 agcaaatgtt gcagtgcgtg ctttggagca gagcgatttt attgtttcta atattgaagc
      781 caaagaaacc agctctcgag ctgctgcacc atttattacc tcatcgttac aacgtgccgc
      841 tagcactcgt ttaggttttg gggttaaaaa gacgatgatg ttagcccaac ggttatatga
      901 agcgggttac atcacctata tgcgtactga ttcaaccaac cttagccgtg aagcattagc
      961 aatggctcga ggttatattc aggataattt cggggcgaat tatttgccgg aaaaagcaaa
     1021 tgtgtatcgc agtaaagata atgcccaaga agctcacgaa gccattcgcc cgtcggatgt
     1081 gaatgtcaaa atggcggatt taaaaggaat ggataaagat gccgagcgtt tatacgattt
     1141 aatttggcgt cgtttcttgg cgtgtcaaat gttgcctgct cgttatgatt ctaccgcagt
     1201 cacagtgtct gccggagact atgaactcaa agcaaacggg cgtgtactgc gttttgacgg
     1261 ctggacgaaa gtactgccgg tgcaaggcaa aaatccggaa gatcaggaat taccggcggt
     1321 tgaactgcat caaaaattaa cgcttgatga aattgtgcca agtcagcatt ttactaaacc
     1381 gcctgcacgt tttaatgagg cgtctttggt tgctgagtta gaagagcgaa aaattggccg
     1441 cccttcaacc tatgcttcaa ttatttcgac gattcaagaa cgtggctatg ttcgcaccga
     1501 aaacaagcgt ttttatgtgg aaaaaatcgg tgaagtagtg acggatcgcc tcaacgaatc
     1561 attcaaagat ttaatgaatt atgatttcac tgccaatatg gaagaggtgc tcgaccaaat
     1621 tgcagagggg tctaaaaatt ggaaagaaga gttgaatact ttctttaaac atttctctga
     1681 agagttgagc caagcagaat tagatgaact tgaaggcgga atgaaaccaa acagccttgt
     1741 gccaaccgat attgattgcc cgacttgtgg acgaaaaatg gcaattcgca ccgcttccac
     1801 aggggtattt ttaggttgca ccggctatgc tttaccgcca aaagagcgtt gcaaaacgac
     1861 aatgaattta attccagagg cggaattttt caatgtgtta gattcggatt ctgaaaccaa
     1921 agccctaatg gctcgcaagc gttgcccgaa atgcgattcg gcaatggata gttatgtgat
     1981 tgatgctgaa cgtaaattac acgtttgtgg taataatcca aattgtgatg gtcatattat
     2041 tgagcaaggt tccttcaaaa ttaaaggcta tgacggacct gtagtggaat gcgataaatg
     2101 cggttcagat atgcacttaa aactgggtcg tttcggcaaa tatatgggct gcacaagctg
     2161 cgataacacc cgtaaaattc tgaaaaacgg ggaagttgct ccgccaaaag aagatcctat
     2221 tgcttttcct gaattaaaat gtgaaaaagc cgatgcctat tttgtattaa gagacggagc
     2281 aagtggggtg tttatgtcag ctcataactt cccgaaagtg cgtgaaagcc gtgcaccaaa
     2341 agtggtagag ttagctcaat atcgtgaacg tttgccggag aaattgcagt atcttgccga
     2401 tgctccgcag caagatccgg aaggaaacga ggcaattatc cgttttagtc gtaaagagaa
     2461 acgccaatat gtgacttctg agaaaaacgg caaagccacc aaatggattg ttgattatct
     2521 agacggtaaa tgggttgagc gtaagaaata aagcttaaaa gacaagcggt aaaattttct
     2581 aaaaatttta ccgcttgctt ttagatgttc tgagcgagta gttcatcaat tgctttactt
     2641 aatttattgc gatcgtgtcg gtaataaacg tcattcgcag ataagttttt tcgcaagatt
     2701 tgaatatgtt cgggaagttc agaaccggca tcatcagtcg taattaccgc attaattcta
     2761 gcctgtccga tacagttctc catccattca attcgctgat taagtgatag ttgcccagct
     2821 gggccatgtt cttgtccgat attgtcaata aatactactc gggctttgct ttgagcaata
     2881 gagtttgcta tgcctgccac cagcaaattc ggcataatac tggtaaaaaa gctacctggg
     2941 cctagcagta ttaactcagc actttgaata gctgaaatgg cttctggtgt agcaacaacc
     3001 atagtatcta acgctaaatg ttgtggtgtt tctgctaaag aatcaatttg cacttcacca
     3061 aatactgatt cgcctgaatg caactgtgca attaaatgca ccggtatttc agacattggg
     3121 ataagttgcg atcttacgtg caataattct cgcaccaaat taatcccttc tagcggtcgg
     3181 atttgcatat tttcaagggc ggtgagaatt aaattgccta gattatgtcc agcgagttcg
     3241 cctgtgccag caaaacgata ctcaaacaac cgagaagcta tcgttggttt aacgataatt
     3301 tgatttaaac agttgcgtaa atctccccaa gcaatcaccc cttgttgtga acgtattcta
     3361 ccggttgagc ctccattatc ggtagtggca acaatgccgg taagccgttc ctttaaaaaa
     3421 gagagcgcgg agagcaatcg acctaagccg tggcccccac ctagtgcaac aactgcattg
     3481 agttggttta aatagtgatg tcgggtaggt ttaaaaggaa taatatctgt attttccatc
     3541 ttgggatttc gtttattttt ctaaaataat ggcatctgac tgggctgaag gcaatgcttg
     3601 taatacagct ttaaccaaag tcgctaaagg aatcgcaaaa aacactcccc aaaagcccca
     3661 taaaccacca aagatgagta cggcaataat aattgtcaga gggtgtagat ttactgcttc
     3721 agaaaataaa aagggtacga gtaaatttcc gtctagcaac tgactaataa taaatgcgag
     3781 cattaaatag tagaaatccg gcgataaccc gaactggaat agagcaatta acattaccgg
     3841 aatgctgact aaaaccgcac cgatgtaggg aactaacact gaaatcccta ccgctacaga
     3901 aagtagtaac ggatagcgta aatcaaaaaa gaggaaaata atataagtga caaccccaac
     3961 aattagaatt tctaaaaatt tacctcgaat atagttggca atttgttgtt gcatttcaaa
     4021 ccatactcgt gttgcaagtc gacggttttg cggtaatact ttactaaaag aacgcattaa
     4081 aacgatttta tctttcagta agaaaaagac catgagtggg actaagaagg tgtaaatgcc
     4141 taagccaact aagctgatga tagagttaat cgagagtgtt agaagtgatt ctcccatact
     4201 caaaatatta cttttagcgg tattcacaat ggaatctagg gtcgcataat ctactaattc
     4261 agggtaatgc tccggtaggg cttgtaacca agcattgagc agattaaaca tcgaaggtaa
     4321 atcttgaata aaggtaactg cttgattcca aaggctgggt agtagtacta cgcctaaaaa
     4381 agataataaa gcgatgaaac ctcctagtac tagaatgaca ctcaaagttc gaggtaattt
     4441 gagtttgctc gacaaaaaac ggattggcca ttccaataaa taggcaaaca caagtgcaac
     4501 cagaaggggc ataagcagat cgctaaaaaa atagataata ccgaaaccga tcaatagaat
     4561 actaaacagc acaactattt gcgggtcggt aaattttttt cgataccaat cttgcaacat
     4621 ttgaaacata tggattgcct ttaaattatg cgatgaaaat tatttctggc tacagactgc
     4681 aatgaaccct agctttttgt ccgggtcatt aaatagtttg tacattttat taaaggtttc
     4741 gcgattttct ggttttaatg cattgccgat aattttcatg gcgcctaaaa caccttcatc
     4801 atgaatcaga cctttagggg aaagtagaga catttctccg gaaaaggtat caacattacg
     4861 gaaaccacaa cgttggaata atgatttcca gtctgcttta gcaagtggag agacagtgat
     4921 attgattgca tcacgcaact cggctaataa agtatcggag tctttatctt ggataacaat
     4981 atcgtgcgtt aataaaaagc catttggttt taatacacga aaatattcac gtattgcctt
     5041 ttctttcgct tctagaggaa gcattgtcag catggcttca ttaattacaa tatcaaaact
     5101 ttcgtcgtca aatggcagtt ttgttgcgtt ggcgcgctgt acttgaatta aatgctcaac
     5161 atggttttct ttgatatttt tacgtgcttt ttcaagcgca tcttcgtcta aatcaatacc
     5221 aacaatttgg cattcaaatt gttgagctaa ttggattgca gtagtcccca tgttacaggc
     5281 aacttcgagc acttttttat ttttatcaaa atccccgttt gcaattaacc aatcagttgc
     5341 taatttcccc ccggggcgta aacgagtttt gcctaaacga gctaaaaaat gatgaccaac
     5401 ttcttctttt ttcataaaaa cctctggatt tgcaaaatac gttaaatata tgaccgcttg
     5461 tattgtgatc tatgttcgtt attatactca attttttcat tttcttatat acaacatcaa
     5521 cagattttgc cagaatacaa cctcattttg atatgaggaa aatagaatga gtaagcaaaa
     5581 gaatgattac ctccaccagc gaggtcaaat taaagaaagt gcagtaaaag cattagtgac
     5641 tgatccactt tttagacaac gtgtagaaag aaatcgtaaa gggaaaggga gctatcgcag
     5701 acaagaaaaa cacaaaaaaa ctatctatgg gcaaaaagaa accccattta aaaatattcg
     5761 gttagagtct ttttaaatgg ggttttattt gtagcttatt gtagttttat aagcctgata
     5821 aacactatgg tttactgaaa taggccaaca acggttctgg attttgaata taagaagaaa
     5881 gttctttatg ctctgggtga cccatatagg gaatttgccc aagtaaaggc gcattaattt
     5941 tttgtgtcag tagttcaatt aattcgtgat aatgccgtag ccctggattt actctgtttg
     6001 cgacccaact tactaatttt acaccacgct gcaaaatttc attcgcagta agtagtgcat
     6061 ggttaacaca accttcctta attcccacca ctaaaacaac cggcatattg ttttgcatta
     6121 cccaatcagc aaaatagtaa tccttgttaa taggagttag ccagccatag gttccttcta
     6181 cgacaacatt tggataggca ttttctagtc gttttaaatc tgtgtttaat ttttccagtt
     6241 gaatatgatg aactgcatct aaagctgcaa atacaggggt actggaatga ataaaagtat
     6301 agctattaat atcacgatag ctaacattag ttggacagct atctagtatg gttaatacgt
     6361 ctgaattatc ttcagtagca taatcatatt gagtaggttc tgtgggtaat gaatcatcac
     6421 cgccacaagc aatgggttta tagccaatta caggaaaact atgttttgta agtgtctgta
     6481 tgatggctcg ggtaaccaca gttttcccta cgttggtgtc tgttccggta ataaataatg
     6541 agggcataaa tggctccttt cgaaaatatg gctggtagtt taaccaaaaa tagatcaatt
     6601 tattctgtct ttgatcaaat ttccgtcata aattccttgt tttattgctg aacaggcaat
     6661 taatggacta ttccaatcgt attggctggt aactaaatcg atttttaggt ttgtttgtaa
     6721 caaattttta tgcaattttt ctgaaatttg cgtaaataac ggctctttta ttttgatcaa
     6781 tggcgagttt agcataattt tttcactaga aaatatatta actaaattca ttaatgcata
     6841 agctaaatta tcggtaatat gcgataaaat agctaatgca agcggttgat tttcatcaat
     6901 tttttgacaa agccagctga ttttctgttc ctgacttttt aaattattag gcaaataacg
     6961 atcaatgagt ctcacaatag ctgggaacgt cacctgattg actaatttat agcgatgaat
     7021 ttcatctagt tcagggaata tttcatcact gagatggcta aatctaggca taagcatatt
     7081 atcaacattc atctttgatt gtttagacag taaactacca ctaagcaata cacttagatt
     7141 cacagtatca tctaattgta gaaaaatagc attatcttgg ttaattaatg agccaaccgt
     7201 agattctgca agcaaccaca gctgaaaatg ttcacttaat aaaataggac aatgaaacag
     7261 cggcgagagt ttagatgtaa tgttacagaa tatatttttg tttcccaatt gagtaatact
     7321 gtctttttct gcattaattt taccgatcac actaatagat acagccagga gctgactagt
     7381 attaacacaa tatagttgg
//
LOCUS       DANLRK010000127         7362 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.127, whole genome shotgun sequence.
ACCESSION   DANLRK010000127 DANLRK010000000
VERSION     DANLRK010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7362
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.127"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            20..2167
                     /locus_tag="PXA33_001931"
     CDS             20..2167
                     /locus_tag="PXA33_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HDL5121239.1"
                     /translation="MKRLRYSYLALILGSALPLSAYADGYLSNDNIRQFDARFNFFLA
                     NPLPAPIAKADGRGEAIENLVVKYASDYLNDIYLKEDVIRFRRWQWQDLYTSLDLKTK
                     QKPDYLNTYRLQAEAYFINKQYQEALSQLDQILRRNPNDVHAMAMSVVASRVLENPNT
                     EAERLLALYQISPSIAQKVQQYLHFTDTMLAASYGSEPQTTMIPDTIAVFGQSPNPDG
                     TPSKGLLQRLEKTKEMAEKFPNAKIILSGGPVRYIYAEADVMKKWLIENGVDESRLLL
                     DDVARDTPGNAVGIIDFMKEYGGRKVLGIGTILHLPRAMSVLKSYADSIGYELEIDSA
                     GGGSPPNEKNKKGEALYTFVNVARAMGLFTLGDFENLLAKEQAEKEQREALEKAQQNY
                     LEKLVAERDQAVKNAENSANELEVARQALETAKQAHQQAEEELNKELSKTQEQLKQST
                     EALSRLIIKQNNPFSEHYVVQLNLMEQATYDLYRRLAEIEHTQDWNLWLSDSLSKYRL
                     KNRSTEDYHLLHIGLDHQVSHNWSIGGVFGISQGQIENRTHKVKGTSAGIYAGYRPTS
                     GLFWTNFFNYSHFTNQSGVTKRDNNAYSLSSEIGRNIVMSHQWQITPKLQLGWAKAGS
                     ARMFWAGTGLRLGKVIELNAIKLKPYADISYRFRNRHIDTLDSLSDRHRNVISIGIQA
                     VGNRHYFAIEGSMLHGDALKRSYGLHANYHYQW"
     gene            2180..3298
                     /locus_tag="PXA33_001932"
     CDS             2180..3298
                     /locus_tag="PXA33_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDL5121240.1"
                     /translation="MKKIKTTLSALALSLSLISVNSWAIPADYLSQDNISQYDKAFNF
                     VLAKVLPAPKGKEDGRGAPIEHLVIQYAPDYVDDPYLKEDVLNFRRWYWQAMYASLDQ
                     KVKEKPTYLNTYRLQAEAYFVNKEYREALSQLDQILRQNPRDIHALAMTVLASRVLED
                     TEQETARLSALKQLAPEVAQRMTDFFAFTEQTLAASYGNEPQTAMIPDTIAVFGSTPN
                     KDGTPSPLLLDRLQKTKEMAQKFPQAKIIVSGGAVKTEFAEADVMAKWLSENGIEKDR
                     LLLDPVARDTPGNAVGIIKLMQTYNGKKVLGIGTLQHIPRATAVLKGYAQHIGYPIEM
                     DSVGGGKPATPGKAKIEALYTYVNVARSMGLFELGDFK"
     gene            complement(3359..4621)
                     /gene="rho"
                     /locus_tag="PXA33_001933"
     CDS             complement(3359..4621)
                     /gene="rho"
                     /locus_tag="PXA33_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HDL5121241.1"
                     /translation="MHLTQLKNTPVSKLVEMGENQMGLENLARLRKQDIVFAILKQHA
                     KSGEDIFGQGVLEILPDGFGFLRSSDSSYLAGPDDIYVSPSQIRRFNLQTGDKIEGKI
                     RPPKEGERYFALLKVDSVNDDKPEASRSKILFENLTPLHANSRLKMERGNGSKEDLTA
                     RILDLAAPIGKGQRALIVAPPKAGKTVLLQNIAQSITHNHPECELIVLLIDERPEEVT
                     EMQRTVRGEVIASTFDEPATRHVQVAEMVIEKAKRSVEHKKDVVILLDSITRLARAYN
                     TVTPVSGKILSGGVDANALHRPKRFFGAARNVEEGGSLTIIATALVDTGSKMDEVIFE
                     EFKGTGNMELHLSRRIAERRIFPAIDFNRSGTRKDDLLMAADELQKTWMLRKVLNPMD
                     EVDAIEWLIDKLMMAKTNEEFFEIMKRS"
     gene            complement(4807..5754)
                     /gene="cysK"
                     /locus_tag="PXA33_001934"
     CDS             complement(4807..5754)
                     /gene="cysK"
                     /locus_tag="PXA33_001934"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015939738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HDL5121242.1"
                     /translation="MTIYADNSYSIGNTPLVRLKNFGNGNIVVKVEGRNPSFSVKCRI
                     GANMVWQAEKDGILTKDKEIVDATSGNTGIALAYVAAARGYKLTLTMPETMSTERKRL
                     LRGLGVNLVLTEGAKGMKGAIAKAEEIAASDPDRYVTLKQFENPANPAIHQQTTGPEI
                     WDATEGKIDVLVAGVGTGGTITGVSRAIKQDKGKQIISVAVEPSESPVITQTLNGEEV
                     KPGPHKIQGIGAGFIPKNLDLSLLDRVEQVSSEEAITTARRLMAEEGILGGISSGAAV
                     AAADRIAKLPEFADKLIVVILPSASERYLSTLLFEGIEA"
     gene            complement(5928..>7362)
                     /gene="pepA"
                     /locus_tag="PXA33_001935"
     CDS             complement(5928..>7362)
                     /gene="pepA"
                     /locus_tag="PXA33_001935"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HDL5121243.1"
                     /translation="VYEPRRLCSAAEQLDKLSNGYISTLLRRGDLEGKAGQTLLLHNV
                     PNVLADRVLLVGCGKERELTERQYKQIIQKSVQTINETGSMEAVSFLTELHVKGRTPY
                     WNVRFAVEAIQEQLYAYNDFKSIKPEVRRELRRVIFNVAHRKDLPDAERALEHGKAVA
                     LGISHAKNVANCPPNVCNPHYLAELAKGLAYDYPAISTTIVDEKQMAELGMNAYLAVS
                     RASENEAFLSVIEYKGNPNADAKPIILVGKGMTFDSGGLSIKPAEAMDEMKYDMCGAA
                     TVYGTIKAIAEMKLPLNVIGLLAGCENAVDGKAYRPGDILTTMSGLTVEVLNTDAEGR
                     LVLCDALSYVERFEPELVIDIATLTGACMIALGNHNSGLMSPHNGLANELLAAAEQAD
                     DKAWRLPLGEEYQEQLKSNFADLANIGGRLGGACTAGQFLSNFTKKYTWAHLDIAGTA
                     WKSGAAKGATGRPVSLLTQFLINKAQV"
BASE COUNT         2113 a         1477 c         1609 g         2163 t
ORIGIN      
        1 aatataagga gaaatcataa tgaaacgctt gcgttattct tatcttgctt tgatattagg
       61 ttcagcctta cctttatcgg cttatgctga cggttattta agtaacgata atattcggca
      121 atttgatgcc cgttttaatt ttttcctcgc aaatccacta cctgcgccta ttgcaaaagc
      181 agatgggcgt ggtgaagcga ttgagaattt ggtggtgaaa tatgcatcag attatcttaa
      241 tgatatctat cttaaagagg atgttattcg ttttcgccgt tggcagtggc aagatctcta
      301 tacgtcattg gatttaaaaa cgaaacaaaa gcccgattat ttaaatactt accgattaca
      361 ggcggaagcg tactttatca ataagcaata ccaagaagca ttatctcaac tggatcaaat
      421 tctgcggcgt aaccctaatg atgttcatgc aatggcaatg agtgtggttg caagtcgggt
      481 attggaaaat cctaatactg aagccgagcg tttattagcg ctttatcaaa tctctccaag
      541 tattgcgcaa aaggtgcagc aatatctaca ttttacagac actatgcttg ctgccagcta
      601 tggtagtgag cctcaaacaa caatgatccc cgacaccata gcggtatttg ggcaatcgcc
      661 taacccagac ggtactccaa gcaagggatt attgcaacgt cttgagaaaa ccaaagaaat
      721 ggcagagaaa tttccgaatg caaaaattat tttaagtggt ggacctgttc gttatatcta
      781 tgcagaagcg gatgtaatga aaaaatggtt gatagagaat ggcgttgatg aaagcagact
      841 gttgcttgat gatgtggcac gagatactcc gggaaatgct gtcggtatca ttgattttat
      901 gaaggaatat ggaggtagaa aagtattggg gatcggtacg atcttacatt taccgagagc
      961 aatgtctgta ttgaaaagct atgcggattc cattggatat gaattggaaa ttgattctgc
     1021 cggaggcggt tctcctccta atgaaaaaaa taaaaaaggt gaagctcttt atacatttgt
     1081 taatgttgct cgtgcaatgg ggttatttac cttgggcgat tttgagaatt tgcttgccaa
     1141 agaacaagct gaaaaagaac aaagagaagc gttggaaaaa gcacagcaga attatttaga
     1201 aaaattggta gctgagcgag atcaagcagt aaaaaatgca gaaaattctg caaatgagct
     1261 ggaagtagcc agacaggcgt tagaaacagc aaaacaggct catcagcaag ctgaagagga
     1321 attgaataaa gaacttagta aaacgcaaga gcagttaaaa cagagtacgg aagcccttag
     1381 tcgtttaata ataaagcaga acaatccttt ttccgagcat tatgttgtac agcttaattt
     1441 gatggaacaa gcaacttatg atctgtaccg taggttagca gaaatagaac atacacaaga
     1501 ttggaattta tggctttcgg atagtctatc caaatatcgt ttaaaaaatc gttcaaccga
     1561 agactatcac ttattacata tcgggctaga tcatcaggtt tcccataatt ggtctattgg
     1621 tggagtattt ggtatcagtc aggggcaaat tgagaatagg acgcataaag tgaaagggac
     1681 atctgctggt atttatgctg gttatcgtcc tacaagtggt ctgttttgga caaatttttt
     1741 taattacagt cattttacta atcaatctgg tgtaacaaaa cgagataata atgcatacag
     1801 tctgagtagt gaaattggaa gaaatatcgt gatgagtcat caatggcaaa ttacgccaaa
     1861 gctgcaatta ggctgggcaa aagctggatc agcacgaatg ttttgggctg ggacagggct
     1921 acgtttagga aaagtgattg aactcaatgc aatcaaactt aaaccttatg ctgatattag
     1981 ctatcgtttt cgaaatcgtc atattgatac cttagatagt ttatctgatc gtcatcggaa
     2041 tgtaatcagt attgggatac aagcagttgg aaatcgccat tattttgcaa tcgaaggatc
     2101 aatgttacac ggggatgcat tgaagcgttc atacggatta cacgcaaatt atcactatca
     2161 atggtaaata ggagcctcta tgaaaaaaat caaaacaacc ctttctgccc ttgctttaag
     2221 cctttctttg ataagtgtaa atagctgggc gataccagct gattatttga gccaagacaa
     2281 tatcagtcaa tatgataagg cgtttaactt tgtattagca aaagtactgc ctgcaccaaa
     2341 aggtaaagaa gatggtcgag gagcgccgat tgagcattta gttattcaat atgcgccaga
     2401 ctatgttgat gacccttatc taaaagagga tgtgctgaat ttccgccgtt ggtattggca
     2461 agcgatgtat gcttcgcttg atcagaaagt taaagagaag ccgacctacc ttaacactta
     2521 ccgtttacaa gcagaggcgt atttcgtcaa taaagagtat cgggaagcac tttctcagct
     2581 cgaccaaatt ttgcgccaaa acccccgtga tattcacgct cttgcgatga cagtattagc
     2641 ttcaagggtg ctagaagata ctgaacaaga aacggctcgt ctttctgcac tgaaacagct
     2701 tgctcccgaa gtcgcacaac gaatgacaga cttttttgcc tttaccgaac aaacgctggc
     2761 ggcaagctac ggtaatgaac cacagacagc aatgatccct gatacgattg ccgtttttgg
     2821 ttctacgcca aacaaagacg gcacaccttc gccgctgttg ttagatcgat tacagaaaac
     2881 caaagagatg gcacaaaaat ttccgcaagc aaaaattatc gtcagtggcg gtgctgtgaa
     2941 aacggaattt gctgaggctg atgtgatggc aaaatggctt agcgaaaatg gtattgaaaa
     3001 agaccgtttg ctacttgatc ctgttgccag agataccccg ggaaatgcgg ttgggattat
     3061 caagctgatg caaacttata atggcaagaa agtattaggt atcggtacgt tacaacatat
     3121 ccctcgtgca acagcggttc tcaaaggtta tgcacagcac attggttatc cgattgagat
     3181 ggattccgtt ggtggtggca agccggcaac accgggaaaa gccaaaatcg aggcacttta
     3241 tacttatgta aatgtggcga gatcaatggg gttgtttgag ttgggggatt ttaagtagtt
     3301 actttattga cagctgctat tgataaaaat cccgcgttaa gcgggatttt tttatgttct
     3361 acgaacgttt cataatctcg aaaaactctt cgttagtttt tgccatcatt aatttgtcga
     3421 ttagccattc gatagcatct acttcgtcca ttgggttgag gactttgcgt aacatccaag
     3481 ttttttggag ttcgtctgct gccattaaga ggtcatcttt acgagtacct gagcggttga
     3541 agtcaatagc cgggaagata cggcgttcag caatacggcg tgagaggtgt aattccatat
     3601 tacccgtacc tttgaactcc tcgaaaatca cttcgtccat ttttgatccg gtatccacaa
     3661 gtgcggttgc gataatggtt aagctgccac cttcttccac attacgggct gcaccgaaga
     3721 aacgttttgg acgatgtaag gcgttagcgt ccacaccacc ggaaaggatt ttgccggaaa
     3781 ccggggtaac ggtgttgtag gcacgggcta aacgagtgat ggagtctaat aaaatcacca
     3841 catctttttt atgctctacc gagcgttttg ctttctcgat taccatctcc gcaacttgaa
     3901 cgtggcgggt tgccggttca tcaaaggttg aggcaattac ttcaccacgt acggtgcgtt
     3961 gcatctctgt tacttcttcc ggacgctcat caattaataa tacgataagc tcacattcag
     4021 gatgattatg ggtgatgctt tgtgcaatat tttgcagtaa cacggtttta ccagcttttg
     4081 gcggggcaac aattaacgct cgctgacctt taccgattgg ggcggctaaa tctaaaatac
     4141 gagcggttaa atcttctttt gatccattgc cacgctccat ttttaagcga gaattggcgt
     4201 gcagaggggt taagttttca aagagaattt tactgcgaga ggcttccggt ttgtcatcat
     4261 taactgaatc aactttcagt agggcaaaat agcgttctcc ctcttttggt gggcgaattt
     4321 taccttctat tttatcgcca gtttgtaagt taaaacggcg gatttggctt ggagaaacat
     4381 aaatatcatc agggccggct aaatatgagc tatctgatga gcggaggaaa ccgaagccgt
     4441 ccggtagaat ttcaagcacg ccttgcccga aaatgtcttc accgcttttt gcgtgttgtt
     4501 taagaatagc aaatacgatg tcttgtttac gtaaacgagc cagattttct aagcccattt
     4561 gattttcacc catttccaca agctttgaaa caggggtgtt tttaagttga gtaagatgca
     4621 taatttgaat gtaaaattgt gattaattaa gattggattt gttgatattg attagatacg
     4681 gccaaccgta aagatgtggc gaagaataac gcaataggag acaaattgca aattttttat
     4741 aaaaaataac cgcttgtttg caataaaaat gccgacatca gccggcattt ttatattaat
     4801 ttaatattac gcttcaatcc cttcaaacag tagcgtactt aaataacgct ctgaggctga
     4861 aggcagaatc actacaatta atttatcggc aaattcaggt agtttagcga tgcgatctgc
     4921 ggcagcaacg gcagcacctg atgaaatacc gcctaaaatg ccttcttctg ccattaaacg
     4981 gcgtgcggtt gtaatcgctt cttcgcttga aacttgttca acacggtcaa gcagtgataa
     5041 atctaagttt ttcggaataa agcctgcacc aatgccttga attttgtgcg ggcccggttt
     5101 gacttcttca ccgtttaaag tttgcgtaat gaccggcgat tcactcggtt ctaccgccac
     5161 tgaaataatt tgcttgcctt tatcttgctt aatagcacgg gaaacaccgg taatagtgcc
     5221 acctgttcct acgcctgcca ctaatacatc aattttacct tcagttgcat cccaaatttc
     5281 aggaccggta gtttgttggt gaattgccgg gtttgccggg ttttcaaact gttttaaggt
     5341 aacatagcga tctgggtcgg aagccgcgat ttcttcagct ttcgcaatcg ctcctttcat
     5401 tccttttgcc ccttcggtta agactaaatt tactcctaag ccacgcaata agcgtttacg
     5461 ctcggtactc attgtttccg gcattgtgag cgttaattta taaccacgag cggctgctac
     5521 ataagccaag gcaattccag tgttaccgct ggttgcatct acaatttctt tgtctttggt
     5581 tagaataccg tctttctctg cttgccacac catattcgca ccaatacggc atttcacgct
     5641 aaaacttggg ttacgccctt ccacttttac cacaatattg ccgttgccaa aattctttaa
     5701 acgcaccagc ggggtattgc cgattgagta tgagttgtct gcatagatag tcatagtgat
     5761 attccttaat aagtattgag attgggattt atcatactta tttttaatcg cttttctagt
     5821 agctggaatt tatttgatat aactaaaaag aatatagggg cgtatgaaga ggatcggtct
     5881 gattttcttt ttgcaaaaac tcgataaaat cagaccgctt gtgagagtta gacctgggct
     5941 ttattgatca aaaattgtgt tagtaaggat accggtctgc cggtcgcccc tttggctgcc
     6001 ccagatttcc aagccgtgcc ggcaatatct agatgcgccc aagtatattt ctttgtaaag
     6061 ttagagagga attgaccggc agtacaggct ccgcctaaac gtccgccgat attagcaaga
     6121 tcagcaaagt tagattttaa ctgttcttga tattcttccc ctaaaggtaa acgccacgct
     6181 ttgtcatcgg cttgctctgc ggcagctaag agttcgtttg ctaaaccgtt atgaggtgac
     6241 atcagcccgc tgttatgatt acctaaggca atcatgcaag caccagttag ggtagcaata
     6301 tcaatcacta attccggttc aaaacgttct acatagctga gagcatcaca tagcactaaa
     6361 cgtccttctg catcggtgtt gagtacctca acggttaaac cagacattgt agtgagaata
     6421 tcccccggtc gataggcttt gccatcaacc gcattttcac agccggcgag taagccgata
     6481 acattgagtg gtaatttcat ctcggcaatt gctttgattg taccgtaaac agtagcagct
     6541 ccgcacatat cgtatttcat ttcgtccatc gcctccgcag gtttaatgga taaaccgcca
     6601 gagtcaaagg tcattccttt gccgactaag ataatcggct tcgcatcagc attcgggttg
     6661 cctttatatt caatcactga aagaaaggcc tcattttcag aggctctgga aaccgctaag
     6721 taagcattca ttcccaattc tgccatctgt ttttcatcaa caatggtagt tgaaatggca
     6781 ggatagtcgt aggctaaacc cttagccaat tcggctaaat agtgaggatt acacacatta
     6841 ggcggacaat ttgctacatt tttagcgtgg ctgattccaa gtgctacggc ttttccgtgt
     6901 tctaaagctc gttcggcatc aggaagatct ttacggtgtg cgacattaaa aatcacacgg
     6961 cgtaattcac ggcgaacttc aggtttgatg cttttgaaat cgttataggc atatagttgt
     7021 tcttggattg cctctaccgc aaatcgcaca ttccaataag gtgttctacc tttaacgtgc
     7081 agttcggtta aaaagctgac agcttccatt gaaccggttt cattaatagt ttgtacactt
     7141 ttttgaataa tttgtttgta ttggcgttca gttaattcac gctcttttcc acagccgact
     7201 aataatactc gatcagcgag tacattcggc acattatgca agagtaaggt ttgccctgct
     7261 ttgccttcta aatctcctct tctcagtaat gtgctgatat agccgttact taatttatca
     7321 agctgttctg cggcagaaca taatcggcgg ggttcataga ca
//
LOCUS       DANLRK010000128         7327 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.128, whole genome shotgun sequence.
ACCESSION   DANLRK010000128 DANLRK010000000
VERSION     DANLRK010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7327
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.128"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            28..447
                     /locus_tag="PXA33_001936"
     CDS             28..447
                     /locus_tag="PXA33_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005704641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS sensor domain-containing protein"
                     /protein_id="HDL5121244.1"
                     /translation="MVPSNEAVLKAVHDTLVISTTDLQGVITYANDLFCKLTGFTREE
                     LIGQPHNIVRHPAVPKEVYKDMWDTIQAGKIWTGIVPNCGKGGVVYVVDTTVQPMFDE
                     TGKITGYISVRRVINDLMTNFEAVESAKEIFDDFYGE"
     gene            518..2074
                     /locus_tag="PXA33_001937"
     CDS             518..2074
                     /locus_tag="PXA33_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase flavoprotein subunit alpha"
                     /protein_id="HDL5121245.1"
                     /translation="MSLIPHIHIAYGSESGNAEKLAQQLAIQPFLLPYSPTLSTLNET
                     DLSQLSAESLLLIVTSSFGDGEPPENADEFAEKLENLTACNLQYAIFGLGDITYDKFC
                     GYSKQLDSLLQQKQAKAVIARVDADLNYQEIFKQWLPLVEQALHSTNKETIQHSLSVQ
                     AYGEEMAYQAEVLEIKHLANSSPAVYHIRLSLKESGIFYQAGDLIYVKVQQPDRLLNE
                     YAEWFNDQNAVALLRHKELRLLSKNVLRDIQKICGSNELKDLTKISNKKALEQYVYGR
                     DLLDVLRDFDPNKSVTLADLEPILSNLAARAYSISSCGKTHPDYVDLCIRHVSYHLNG
                     RDYQGTASGYLSELQAGDFVSIFANANPNFRLPETLTAPVVMIGSGTGIAPHIAFLQA
                     LEQQYQGVESYLFFGERYRDKDFLYQTELENYLAKGTLTQLFTAFSRDQAEKFYVQDA
                     LSQQAELVWHLIQQGAYFYICGSKAMSKAIDAELIKIADQIGGDPYIDDFNNIIAKLV
                     AEGRLMRDVY"
     gene            complement(2132..2389)
                     /gene="rpsQ"
                     /locus_tag="PXA33_001938"
     CDS             complement(2132..2389)
                     /gene="rpsQ"
                     /locus_tag="PXA33_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005759643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HDL5121246.1"
                     /translation="MTDKIRTVQGKVVSDKMDKSFVVAIERTVKHPLYGKFIRRTTKL
                     HVHDENNEAKLGDVVEIKECRPISKTKSWTLVRVVEKAVVA"
     gene            complement(2389..2580)
                     /gene="rpmC"
                     /locus_tag="PXA33_001939"
     CDS             complement(2389..2580)
                     /gene="rpmC"
                     /locus_tag="PXA33_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HDL5121247.1"
                     /translation="MKAQELRNKSVEELNGELVNLLGEQFKLRMQAATGQLQQTHQLK
                     QVRRSIAQVKTVLTEKAGE"
     gene            complement(2580..2990)
                     /gene="rplP"
                     /locus_tag="PXA33_001940"
     CDS             complement(2580..2990)
                     /gene="rplP"
                     /locus_tag="PXA33_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HDL5121248.1"
                     /translation="MLQPKRTKFRKVHKGRNRGIAGGTEVSFGSFGLKAIGRGRLTAR
                     QIEAARRAMSRAVKRQGKIWIRVFPDKPITEKPLEVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVSEEVARNAFALAAAKLPFKTTFVTKTVM"
     gene            complement(3004..3711)
                     /gene="rpsC"
                     /locus_tag="PXA33_001941"
     CDS             complement(3004..3711)
                     /gene="rpsC"
                     /locus_tag="PXA33_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HDL5121249.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTQDFADNLDGDFKVRKFLN
                     KELANASVSRITIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRNAVAKIAGVPAQIN
                     IAEVKKPELDAKLVADSIASQLERRVMFRRAMKKAVQNAMKLGAKGIKVEVSGRLGGA
                     EIARSEWYREGRVPLHTLRADIDYSTAEAHTTYGVIGVKVWIFKGEILGGMAAVIESE
                     KEPAAQPKKAPRGKGRK"
     gene            complement(3731..4063)
                     /gene="rplV"
                     /locus_tag="PXA33_001942"
     CDS             complement(3731..4063)
                     /gene="rplV"
                     /locus_tag="PXA33_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HDL5121250.1"
                     /translation="METIAKHRYARTSAQKARLVADLIRGKKVSVALEILTFTNKKAA
                     TLVKKVLESAIANAEHNDGADVDDLKVAKIFVDEGPSMKRVMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            complement(4074..4349)
                     /gene="rpsS"
                     /locus_tag="PXA33_001943"
     CDS             complement(4074..4349)
                     /gene="rpsS"
                     /locus_tag="PXA33_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HDL5121251.1"
                     /translation="MPRSLKKGPFLDLHLLKKVEKAVESGDKKPIKTWSRRSMIIPSM
                     IGLTIAVHNGRQHVPVYVSDEMIGHKLGEFAPTRTYRGHAADKKAKK"
     gene            complement(4380..5201)
                     /gene="rplB"
                     /locus_tag="PXA33_001944"
     CDS             complement(4380..5201)
                     /gene="rplB"
                     /locus_tag="PXA33_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HDL5121252.1"
                     /translation="MAIVKCKPTSAGRRHVVKVVNAELHKGKPFAALLDTKSKTGGRN
                     NLGRITTRHIGGGHKQHYRLIDFKRNKLDIPGVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLTAGDQIQAGASAPIKVGNALPMRNIPVGTTVHNVELKPGKGGQIARSAGA
                     YVQIIAREGNYVTLRLRSGEMRKVLAECTATIGEVGNSEHMLRVLGKAGANRWRGIRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRHNKRTDKFIVRRRGK"
     gene            complement(5220..5525)
                     /gene="rplW"
                     /locus_tag="PXA33_001945"
     CDS             complement(5220..5525)
                     /gene="rplW"
                     /locus_tag="PXA33_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005818317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HDL5121253.1"
                     /translation="MIQQERLLNVLKAPHISEKATNNAEKGNTIVFKVALDANKVEIA
                     NAVEELFEVKVDSVRTVVVKGKTKRRGAKVGRRSDWKKAYVTLQEGQSLDFVEGAAE"
     gene            complement(5522..6124)
                     /gene="rplD"
                     /locus_tag="PXA33_001946"
     CDS             complement(5522..6124)
                     /gene="rplD"
                     /locus_tag="PXA33_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HDL5121254.1"
                     /translation="MELQVVGANALTVSETTFGREFNEALIHQVVVAYAAGARQGSRA
                     QKTRAEVSGSGKKPWRQKGTGRARSGDIKSPIWRSGSVTFAAKPQDHSQKVNKRMYRG
                     AIKSILSELVRQDRLVVVEKFEIDAPKTKVLAQKLKDMALNDALIITANVDENLFLAA
                     RNLYKVDVRDVQGIDPVSLIAFDKVVVTVDAVKQIEEMLA"
     gene            complement(6140..6769)
                     /gene="rplC"
                     /locus_tag="PXA33_001947"
     CDS             complement(6140..6769)
                     /gene="rplC"
                     /locus_tag="PXA33_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HDL5121255.1"
                     /translation="MIGLIGRKVGMTRVFTEDGVSIPVTVIEIEANRVTQVKTLENDG
                     YSAIQVTTGSKKASRVTKPEAGHFVKAGVEAGRGLWEFRTTEGEEFTLGQEINVDIFT
                     DVKKVDVTGTSKGKGFQGGVKRWNFRTQDATHGNSLSHRVLGSIGQNQTPGRVFKGKK
                     MAGHLGAERVTVQSLEVVRVDVERKLLLVKGAVPGATNGDVIVKPAVKA"
     gene            complement(6795..7106)
                     /gene="rpsJ"
                     /locus_tag="PXA33_001948"
     CDS             complement(6795..7106)
                     /gene="rpsJ"
                     /locus_tag="PXA33_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HDL5121256.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQSTAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHKRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
BASE COUNT         2129 a         1562 c         1362 g         2274 t
ORIGIN      
        1 taatggagat tcaacacggc tgaacaaatt gttccttcaa atgaagctgt tttaaaagca
       61 gtccacgata ctttagttat ttcaacaact gatttacaag gtgttattac ttacgctaac
      121 gaccttttct gcaaattaac cggttttacc cgtgaagaat taattggaca accgcataat
      181 atcgtacgcc acccagctgt accaaaagaa gtttataaag atatgtggga tacaatccaa
      241 gccggtaaaa tctggacagg tatcgtacca aactgcggta aaggcggtgt agtttatgtg
      301 gttgatacca ccgttcaacc aatgtttgat gaaacaggca aaattacagg ctatatcagc
      361 gttcgccgtg taattaatga tttaatgacc aactttgaag cagtagaatc agcaaaagaa
      421 atctttgatg acttctacgg tgaataatta aaaaacaatg attgcgtgta aaagatgatc
      481 tcttttacac gcaatttttt attttcgagg agtttgtatg tctctcattc cccatatcca
      541 cattgcttat ggttcggagt ctggtaacgc agaaaagctt gcccaacaat tagctatcca
      601 acccttttta cttccctact cacccacact ttctacatta aacgaaaccg atttaagcca
      661 attatctgcc gagagtttat tgcttattgt aaccagctcc tttggtgatg gcgaaccacc
      721 tgaaaacgcc gatgaatttg cagaaaaatt ggaaaatttg accgcttgta atctgcaata
      781 tgcgatattc gggctaggtg atattactta tgataaattc tgtggctata gcaaacaact
      841 agattcatta ttacaacaaa aacaagctaa agctgtgatt gcaagggttg atgccgattt
      901 aaactatcaa gaaattttta aacaatggct acccctagtg gagcaggcat tacattcaac
      961 aaacaaagaa acgatccaac attccctttc tgttcaagct tacggcgagg aaatggcata
     1021 ccaagcagaa gtgttggaaa ttaagcattt ggcaaactca agccctgcgg tgtatcacat
     1081 tcgtttatca ttaaaagaga gcggtatttt ctatcaagca ggggatttaa tttatgtcaa
     1141 agtgcagcaa ccagacagac tattaaatga atatgcagaa tggtttaacg atcaaaatgc
     1201 agttgcatta ctacgccata aagaactccg actactaagt aaaaatgtac tgcgtgatat
     1261 tcaaaaaatt tgcggcagta atgaactcaa agatttaact aaaatttcca ataaaaaagc
     1321 attggaacaa tatgtctatg ggcgggattt attagacgta ttacgcgatt ttgacccaaa
     1381 caaaagcgtg acattagccg atttagagcc aattttatct aacttagccg cacgagccta
     1441 ttcaattagc tcttgcggta aaactcaccc tgattatgtt gatttatgta tccgccacgt
     1501 ttcgtatcat ttaaacggta gagattatca agggactgca agtgggtatt tatccgaatt
     1561 acaagcgggc gattttgtct cgatttttgc aaatgccaat ccgaatttcc gtttacccga
     1621 aacattaacc gctccggtgg tgatgattgg ctcaggtact ggcattgctc cgcatatcgc
     1681 atttttacaa gcgttagagc agcaatatca gggggttgaa agctatttat tcttcggcga
     1741 gcgttatcgt gataaagatt ttctgtatca aacggaactg gaaaattact tagcaaaagg
     1801 caccttaact cagctgttta ctgctttttc acgtgatcag gcagagaagt tttatgtgca
     1861 agatgcccta tcgcaacaag cagagttagt ttggcattta attcagcaag gggcttattt
     1921 ctatatttgt ggtagcaaag caatgagtaa agcaatagat gcggaactca tcaaaattgc
     1981 agatcaaatt ggaggcgatc cttatattga tgatttcaat aatattattg caaaattggt
     2041 cgcagaaggc cgattaatgc gagatgtata ttagcaaatg aaaaaaacta aaaacccagc
     2101 aaaagctggg tttttattta atcatagttc attaagcaac aactgctttc tcaactacac
     2161 gaactaaagt ccaagattta gtttttgaaa taggacgaca ttccttaatt tcaacaacat
     2221 cacctaattt agcttcattg ttttcatcgt gtacatgaag tttagtagta cgacggataa
     2281 acttaccgta taacggatgt ttaactgtac gttcgatagc aacaacaaat gatttatcca
     2341 ttttgtcgct aaccacttta ccttgtactg tacgaatttt atcagtcatt attcacccgc
     2401 cttttcggtt aatacagttt taacttgtgc aatactacga cgcacttgtt ttaactggtg
     2461 ggtttgttga agctgaccgg tggctgcttg catacgcaat ttgaattgtt cacctaaaag
     2521 gttcactaac tcaccattca gctcttcaac acttttatta cgtagttctt gagctttcat
     2581 tacatcaccg tcttagttac aaatgtggtt ttgaacggaa gtttagctgc cgctaatgca
     2641 aacgcattgc gtgcaacttc ttcagaaaca ccatccattt catagagaac tttacccggt
     2701 tggattaagg ctacccagta ctcaacgtta cctttacctt tacccatacg gacttctaat
     2761 ggtttttcag taattggttt gtctgggaac acacggatcc agattttacc ttgacgttta
     2821 accgcacggc tcatcgcacg acgagcagct tcaatttgac gagctgttaa acgaccacgg
     2881 ccaatagctt ttaaaccaaa tgaaccgaag ctcacttctg tgccacccgc gataccacgg
     2941 ttacggcctt tgtgtacttt acggaatttt gtgcgttttg gttgtaacat tcagtgattc
     3001 tccttattta cgacctttgc cacgtggcgc ctttttaggc tgtgccgctg gttctttttc
     3061 agattcaatc actgcagcca ttccaccaag aatttcacct ttgaagatcc acacttttac
     3121 gccgattacg ccgtaagtag tgtgagcttc agcagtacta taatcgatgt ctgcacgaag
     3181 cgtatgtaga ggtacacgac cttcacgata ccattctgag cgagcaattt ctgcaccacc
     3241 taaacgaccg ctaacttcaa ctttgatacc tttagcacct aatttcatcg cgttttgaac
     3301 tgcttttttc attgcacgac ggaacatcac acgacgttca agttgagaag cgatgctatc
     3361 tgcgactaat ttcgcatcta gctctggttt tttcacttca gcgatgttga tttgagctgg
     3421 aacgccagcg attttcgcaa ctgcgttgcg taatttttca acatcttcac cttttttacc
     3481 gataacgata cccgggcgag ctgtgtgaat agttacacga atgctttttg caggacgctc
     3541 aatagtgatg cgtgatacag aagcgttcgc taactctttg tttaaaaatt tacgtacttt
     3601 gaagtcgccg tctaagttat cagcgaaatc ttgtgtattc gcgaaccaag tagagttcca
     3661 aggtttaaca atacctaggc gaataccatt aggatgtact ttctgaccca tttgttattc
     3721 tcctacttaa ttaacgatct gacacaacca cagtgatgtg gcttgtacgt tttaaaatac
     3781 gatctgcacg accttttgca cgtggcatta cacgtttcat gcttggacct tcatcaacga
     3841 agattttagc aactttaaga tcatcgacat ctgcaccgtc attatgctct gcgtttgcaa
     3901 tagctgactc taatactttc ttaacaagcg tcgctgcttt tttgttagtg aaagttaaga
     3961 tttctaatgc tacagaaact tttttaccgc gaattaaatc agcaactaag cgagcttttt
     4021 gggcagaagt acgagcgtaa cgatgtttag caatagtttc catcttttac ctcttatttc
     4081 ttagctttct tatctgcggc gtgaccgcgg tatgtacgag tcggtgcaaa ttcacctaat
     4141 ttatgaccga tcatttcgtc ggaaacataa acaggaacgt gctgacgacc attatggact
     4201 gcgatggtca aaccaatcat tgatggaatg atcattgaac gacgggacca agttttaatt
     4261 ggttttttat ccccgctttc caccgccttc tctaccttct tcaacaagtg taggtcaagg
     4321 aaaggacctt tcttgaggga acgtggcatt ggtgtttacc ttttagttaa tgaattaaat
     4381 tatttgccac gacgacgtac gatgaattta tcagtacgtt tgttgtgacg agttttctta
     4441 cctttggttt gaacgcccca aggtgtaaca ggatgtttac cgaagttacg accttcacca
     4501 ccaccgtgtg ggtgatctac cgggttcatt gcagtaccac gaacagttgg acgaatacct
     4561 ctccaacggt ttgcacctgc tttaccaaga acgcgaagca tatgctctga gttacctact
     4621 tcaccaatgg ttgcagtaca ctcagctaat actttacgca tttcgcctga acgaagacgt
     4681 aaagttacat agttaccttc acgggcaatg atttgaacgt aagcaccagc agaacgtgca
     4741 atttgaccac ctttaccagg ttttaattca acgttgtgta cagttgtacc tactgggatg
     4801 ttacgcattg gtaatgcgtt acctacttta atcggagctg aagcacccgc ttgaatttga
     4861 tcaccagcag ttaaaccttt tggtgctaaa atatagcgac gttcaccatc tttataaagt
     4921 actaatgcga tattcgcaga acgatttgga tcgtattcta aacgctcaac aacacccgga
     4981 atgtctaatt tattacgttt aaaatcaatt aaacggtagt gttgtttgtg accgccacca
     5041 atgtgacgag ttgtgatacg acctaagttg ttacgaccac cagttttaga tttagtatct
     5101 aaaagtgctg caaaaggttt acctttgtga agctcagcgt ttacaacttt aacaacgtga
     5161 cgacgaccag cggaggtcgg cttacattta acgatagcca tttattttat ctcctctgat
     5221 tactctgccg caccttcaac gaagtcaagc gattgacctt cttgaagagt tacataagct
     5281 tttttccagt cactacgacg accaacttta gcaccacggc gtttagtttt acctttaact
     5341 actacggtac gaacagaatc aacttttact tcaaaaagtt cttcaactgc atttgcaatc
     5401 tcaactttgt ttgcatctaa tgcaactttg aatacgattg tattgccttt ttctgcgttg
     5461 tttgtcgctt tttcagagat atgtggtgct ttaagcacgt ttagcaaacg ttcttgttga
     5521 atcatgctaa catctcctca atttgtttta ctgcatctac ggttacaacc actttatcga
     5581 aagcaattaa gctaactggg tcaatacctt gcacatcacg cacatcaact ttatataagt
     5641 tgcgtgctgc taagaataaa ttctcatcta cgtttgcagt aatgataaga gcgtcattta
     5701 acgccatatc ttttaatttt tgtgctaata ctttagtttt tggtgcatcg atttcgaatt
     5761 tctcaacaac cactaaacgg tcttgacgaa ctaattcaga aagaatgctt ttgattgcac
     5821 cacggtacat tcttttgttc actttttggc tgtgatcttg tggtttcgca gcaaaagtta
     5881 cactacccga acgccaaatt ggtgatttaa tatcaccaga acgtgcacgg cctgtacctt
     5941 tttgacgcca aggtttttta cctgaaccag acacttcagc acgagttttt tgagcacgag
     6001 atccttgacg agcacctgct gcataagcaa caactacttg gtggattaat gcttcattga
     6061 actcacgtcc gaaggtagtt tcagaaacag ttagtgcgtt tgcacctaca acttgtaatt
     6121 ccatctctat ctcctagact tatgctttaa ctgccggttt tacgataaca tcaccattag
     6181 ttgcaccagg aacggcacct ttaactaata ataatttacg ctcaacatct acacgaacaa
     6241 cttcaagtga ttgaacggtt acacgctctg cacctaagtg tcctgccatt tttttacctt
     6301 taaacacacg acctggagtt tggttttgac caatagaacc aagtacacga tgtgataaag
     6361 agttaccgtg agttgcatct tgagtacgga agttccaacg tttaacacca ccttggaagc
     6421 ctttaccttt tgatgtacca gtaacatcga cttttttaac atcagtaaag atgtcaacat
     6481 tgatttcttg acctaaagtg aattcttcac cttcagtagt acgaaattcc cataaaccgc
     6541 gtccagcttc aacacctgct ttcacgaaat gacctgcttc tggtttagtt acacggctag
     6601 cttttttaga gccagtagta acttgaattg cagagtagcc atcgttttca agggttttaa
     6661 cttgagtaac acggttggct tcgatttcga taacggtaac tggaatagac acgccgtctt
     6721 cagtgaaaac gcgggtcata ccaactttac gaccgattaa accaatcatt gtaataacct
     6781 cttaaaattc ccaattaacc tagactaatc tgaacgtcaa cgccagcagc tagatctaaa
     6841 cgcattaatg catcaacagt tttttctgtt ggttcaacaa tatcaactaa acgtttgtga
     6901 gtacggattt catattggtc acgtgcatct ttattcacgt gtggagaaat caatacagtg
     6961 aaacgttctt tacgagttgg taaaggaatt ggaccacgaa cttgtgcacc agtacgttta
     7021 gctgtttcta cgatctccgc agtagattga tcaattaaac gatgatcaaa tgcttttaag
     7081 cggatacgga ttctttggtt ctgcattaga ccagagctcc aattaaaaaa tttagctaat
     7141 aaaaaaacca aactaccact taacaaatta gttaagggag ctcagttaaa cctatatagt
     7201 tccaaaatta ggaacattgt atacactaca atatctgtag tgccatttga taaatgatta
     7261 catcaaatgt ctttagatac ctaaagcttt gctatcatac tcgtttcatt tcccttatgc
     7321 aagtaat
//
LOCUS       DANLRK010000129         7294 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.129, whole genome shotgun sequence.
ACCESSION   DANLRK010000129 DANLRK010000000
VERSION     DANLRK010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7294
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.129"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(89..1024)
                     /gene="ftsX"
                     /locus_tag="PXA33_001949"
     CDS             complement(89..1024)
                     /gene="ftsX"
                     /locus_tag="PXA33_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HDL5121257.1"
                     /translation="MIRSFSKLGAQTNYSLRAVWQDLMQRKLGTFLTVLVIAVSLTIP
                     TVSYLLWKNTHEAATQFYPEPQLTVYLHKNLAEHDVNTVVDRIRAFETDKIESFNYIS
                     RQQSLDEFRSWSGFGEALDILDENPLPAVVTLKPKANFNNADTMVELRNGLQQIKGVQ
                     EVRLDNGWLEKLTALTWLIARIAVVCTLLMLLAVFLVIGNSVRTDVANSKAAIEVQQI
                     LGATDHFIARPFLYTGMIYGLFGSLLAVLFSAVTISYFTGVVKYVTDMFTVQFELNGL
                     DFSEAFFLVVLCVFIGWLSAKIATMRNIFRIGSRG"
     gene            complement(1034..1687)
                     /gene="ftsE"
                     /locus_tag="PXA33_001950"
     CDS             complement(1034..1687)
                     /gene="ftsE"
                     /locus_tag="PXA33_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HDL5121258.1"
                     /translation="MIKFTNVNKVYKGGKPALQGIDFHLPAGGMAYITGHSGAGKSTL
                     LKLIMGIERANGGQILFNGHDITRLAHHELPFLRRQIGMVHQDYRLLTDRTILDNVAL
                     PLIILGMNQAIVEREARIALERVGLADKANYLPLHLSGGEQQRVDIARAIVHRPTLLL
                     ADEPTGNLDEKLSFEIFRLFEEFNQAGTTVLIATHDTNIISKRPKPCLVLEQGHLKF"
     gene            complement(1680..3176)
                     /gene="ftsY"
                     /locus_tag="PXA33_001951"
     CDS             complement(1680..3176)
                     /gene="ftsY"
                     /locus_tag="PXA33_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HDL5121259.1"
                     /translation="MSEKKKGFWSWLGFGKKEEAEQKEIQQEQESIQPEQPVIVEPET
                     EKSFSEKLDELEDKIEEKVDQLEDYFEEKEEKIEQFAEQVEDTIEQKVEDFKEFVEEK
                     VEEAKAFFDDVEDKIEQTFEEQKGEQAVENLQNIATEQLDLNEQEQVTYQVEEAVAEE
                     VAETELVNNVQDTVEESTIQDEYQEKPSQGGFFSRLIQGLIKTKQNIGSGFRNFFRGK
                     KIDDELFEELEEQLLVADLGMPTTQKIINNLTQHATKQQLKDADLLYQQLKVELGEVL
                     KPVAQPLVLEAKKPYVILMVGVNGVGKTTTIGKLARKFQQEGKSVMLAAGDTFRAAAV
                     EQLQVWGERNNIPVVAQSTGSDSASVIFDAMQSAAAKGIDVLIADTAGRLQNKNNLMD
                     ELKKIVRVMKKYDETAPHEIMLTLDAGTGQNAISQAKLFNEAVGLTGITLTKLDGTAK
                     GGVIFAIADQFNIPIRFIGVGEKIEDLRPFNAEEFIEALFSHEDEQND"
     gene            3255..3845
                     /gene="rsmD"
                     /locus_tag="PXA33_001952"
     CDS             3255..3845
                     /gene="rsmD"
                     /locus_tag="PXA33_001952"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HDL5121260.1"
                     /translation="MKKNRNSPQSVQQMGEVRVIAGLWRGRKLPVLNAEGLRPTTDRV
                     KETLFNWLMHDIAHSRCLDCFAGSGSLGIEALSRQAQAVVFLEKFANAANQLKKNLQA
                     LKSEQGSVIHTDTLAYLAQKNNGEPFNIVFIDPPFHQGFVPQVLNLLAENNWLAPNAI
                     LYVETEKNHPPLELPQGWQILKEKAAGMVVSRLIQL"
     gene            3929..4243
                     /locus_tag="PXA33_001953"
     CDS             3929..4243
                     /locus_tag="PXA33_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HDL5121261.1"
                     /translation="MKKLTPDEAIDIAYDIFLEMAGENLDPADILLFNLQFEERGAVE
                     MVETSEDWDQEIGTLIDPDAFAEVWVGLVNEQDEMDDVFARFLISHDEENREYHVVWK
                     EK"
     gene            4256..4768
                     /gene="hpaC"
                     /locus_tag="PXA33_001954"
     CDS             4256..4768
                     /gene="hpaC"
                     /locus_tag="PXA33_001954"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     reductase component"
                     /protein_id="HDL5121262.1"
                     /translation="MANIDTNQPIEASLKFREAMAHLSSAVSIVTTNGVAGKAGLTVS
                     SVCSVTDSPPTLLFCINKNSHVHDIIQQNGKVCVNVLSHEQEDLAKHFAAILESTMEE
                     RFGWDIWNNGFDDQPVLRSAISALQGNIVDTHAVGTHTIFVVEVSHIDVEPNHSLVYF
                     ARQFRSVNID"
     gene            4886..5758
                     /gene="tehB"
                     /locus_tag="PXA33_001955"
     CDS             4886..5758
                     /gene="tehB"
                     /locus_tag="PXA33_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HDL5121263.1"
                     /translation="MENLICYKQMPVWTKATLPQMFQEKHNTKVGTWAKLTILKGSLK
                     YYELTEEGEIIKEDIFDANHQRPMVEPQLWHKVEALSDDLECQLAFYCKPEDYFAKKY
                     NLTATHSEVLNAVNYTKGGKALDLGCGRGRNALYLNLLGFDVTAVDHNQESIDFLNYM
                     IEKEQAQNITTAVYDINEATIGSQNGEYDLIISTVVMMFLNRERIPAIIENMQKNTKV
                     GGYNLIVCAMSTDDYPCPMPFSFTFKEGELADYYQGWELVKYNEDLGHLHKTDENGNR
                     IQLRFATMLAKKIS"
     gene            5896..6933
                     /gene="purM"
                     /locus_tag="PXA33_001956"
     CDS             5896..6933
                     /gene="purM"
                     /locus_tag="PXA33_001956"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HDL5121264.1"
                     /translation="MSNTQLSYKDAGVDIHAGNELVERIKPDVKRTRRPEVMGGLGGF
                     GALCALPTKYKEPILVSGTDGVGTKLRLAIDLNKHDTIGQDLVAMCVNDLVVQGAEPL
                     FFLDYYATGKLEVDVATNVVKGIADGCVISGCALVGGETAEMPGMYHEGDYDLAGFCV
                     GVVEKSKIIDGSTVKTGDALIALASSGPHSNGYSLIRKVLEVSRSNPATDLLEDKPLS
                     EHLLAPTKIYVKSILKLVEEVDVHAIAHLTGGGFWENIPRVLPESAKAVIDESSWQWP
                     AIFSWLQEKGNISRYEMYRTFNCGVGMVIALPERDVEKALTLLKESGENAWLIGKIED
                     LGTGSEQVEIR"
     gene            7011..7289
                     /locus_tag="PXA33_001957"
     CDS             7011..7289
                     /locus_tag="PXA33_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HDL5121265.1"
                     /translation="MQLFWPIYFVLMNLAALYLMYSDKQKAIKKEWRVPEITLLSFCL
                     LGGAIGVYLGMKYFRHKTQHWYFHVAVVLSTFAWLIGLPYFYLALSSY"
BASE COUNT         2131 a         1592 c         1414 g         2157 t
ORIGIN      
        1 agcaataata tattgcttat agtaaaaaac caagttccta ggaactggtt tttatctgca
       61 attatttctc taaaaacaac cgcttgtatt agcctctact accaatacgg aaaatatttc
      121 gcattgtcgc aatttttgcc gacagccagc caataaacac acaaagcacc actaagaaga
      181 aggcttcgct gaaatctaat ccgttcagtt caaattgtac ggtaaacata tcagtcacat
      241 acttcactac accggtgaaa tagctaatcg ttaccgcact aaacagcacc gctaacaggc
      301 tgccaaacaa gccgtaaatc atacctgtgt ataaaaacgg acgggcaata aagtgatcag
      361 ttgcccctaa aatttgttgg acttcaatcg ctgctttgct attggcaaca tcggttcgca
      421 cactgtttcc aatcactaaa aaaactgcca acagcattaa taaggtacac accaccgcaa
      481 tacgagcaat cagccaagtt agagcagtta atttttccag ccagccgtta tctaaacgaa
      541 cctcctgcac ccctttaatt tgttgcaaac cgttgcgtag ctccaccatc gtatcagcat
      601 tattaaaatt tgctttcggt tttaaggtaa cgaccgcagg caacgggttt tcatctaaaa
      661 tatctaacgc ttcaccaaag cctgaccaag aacgaaattc gtctaaactt tgttggcgag
      721 aaatatagtt aaatgactca attttatccg tctcaaaagc acgaatgcga tcaacaacgg
      781 tattcacatc gtgttctgcc aaatttttgt gcagataaac ggttaattgt ggctcagggt
      841 aaaattgtgt tgctgcctcg tgggtattct tccaaagtaa ataactcacg gttggaatcg
      901 taagtgagac cgcaatcacc agcaccgtca gaaaagtgcc gagcttgcgt tgcattaaat
      961 cttgccaaac cgctcttaaa gagtagttgg tttgagcacc taatttacta aatgaacgaa
     1021 tcataatatt tccttaaaat ttaagatgcc cttgctccag cactaaacac ggctttggac
     1081 gctttgaaat aatattggta tcgtgggtgg caatcagcac cgttgtgccg gcttgattaa
     1141 attcctcaaa caaacggaaa atttcaaacg agagcttttc atctaagttt ccggttggct
     1201 catcggctaa taacagcgtt gggcgatgaa caatggccct ggcaatatcg acacgttgtt
     1261 gctcaccgcc ggaaaggtgc aacggcagat agttcgcttt atctgccaag ccaacacgct
     1321 caagggcaat gcgggcttca cgctccacaa tcgcttggtt cattcccaaa ataatcaatg
     1381 gtaatgcgac attatccaaa atggttcggt cggttaataa gcgataatct tgatgcacca
     1441 tcccgatttg gcggcgtaaa aacggcaatt catgatgagc aagtcgggta atatcgtgtc
     1501 cgttaaacaa aatttgcccg ccattggcac gctcaatccc cataattaat tttaatagcg
     1561 tgcttttccc cgcaccggaa tgcccggtaa tatatgccat tccaccggca ggtaggtgga
     1621 aatcaatgcc ctgtaaggct ggctttccgc ccttataaac cttgttcaca ttagtgaatt
     1681 taatcattct gttcgtcctc gtggctaaat aaggcttcaa taaattcttc tgcattaaac
     1741 gggcgtagat cttcaatttt ctcgcccacc ccaataaagc ggatcggaat attgaattga
     1801 tcggcaatcg caaaaatcac gccacctttt gccgtaccgt ctaatttagt taaggtgata
     1861 ccggttaaac ccactgcctc attaaacaat ttcgcttggc tgatcgcatt ttgcccagtg
     1921 ccggcatcaa gcgtaagcat aatttcgtgc ggtgcggttt catcatattt tttcatcacc
     1981 cgcacaattt tcttcaactc gtccatcagg ttatttttgt tttgcaaacg ccctgcagta
     2041 tcagcgatta gcacgtcaat cccttttgcc gcagcggatt gcatcgcatc aaaaatcacc
     2101 gaggcagaat ccgaaccggt gctttgtgcc accaccggaa tgttatttcg ctctccccaa
     2161 acctgtagct gttcaactgc cgccgctcgg aacgtgtctc ctgctgctaa cattaccgat
     2221 ttgccttctt gttggaattt acgggcaagt ttgccaatgg tagtggtttt ccccacgcca
     2281 ttcacaccaa ccattaaaat gacatacggt tttttcgctt ccaacactaa cggttgagcc
     2341 accggtttta gcacttcacc cagttccact ttcaactgtt gatacaacaa atctgcatct
     2401 tttaattgtt gcttggtagc atgttgcgtt aaattgttga tgattttttg tgtggtcggc
     2461 atacctaaat ccgccacaag caactgctct tccagctctt caaataactc atcatcaatt
     2521 ttcttgccac ggaagaaatt acggaagccc gaaccaatat tctgcttggt tttaatcaag
     2581 ccttgaatta aacgactgaa gaaaccgcct tgactcggtt tttcttgata ttcatcttgg
     2641 atagtagatt cttcaactgt gtcttgaaca ttatttacta attcagtttc agcaacttct
     2701 tcagccacag cctcttcgac ttgataagta acttgctctt gttcgttgag atctaactgc
     2761 tcagttgcaa tattttgaag attttcgacc gcttgttcgc ctttttgttc ttcaaatgtt
     2821 tgttcgattt tatcttcaac atcatcaaag aacgctttcg cctcttcaac tttttcttca
     2881 acaaactctt tgaaatcttc gactttttgt tcaatggtat cttctacttg ctcagcaaat
     2941 tgctcaattt tttcttcttt ttcctcaaaa tagtcttcta actgatctac cttttcttca
     3001 attttatctt ccagctcatc taatttttca gaaaacgact tctcagtttc cggttcaaca
     3061 ataacaggct gttccggttg aatactctct tgttcttgtt gaatctcttt ctgttcagct
     3121 tcttctttct taccaaatcc aagccaagac caaaaccctt ttttcttctc tgacatattt
     3181 ttctctcatt tctcgtaaaa atagactaaa cttacatagt ctatcaaaaa atcccttatt
     3241 ttcctttagt gagtatgaaa aaaaatcgaa attcccccca atctgttcag caaatgggcg
     3301 aagtacgggt tatcgccgga ctgtggcgag gcagaaaatt acctgtacta aatgccgagg
     3361 ggcttcgccc gacaaccgat agagtaaaag aaacgctgtt taactggtta atgcacgaca
     3421 ttgctcactc acgctgtctt gattgctttg caggcagtgg ctctctgggc attgaagccc
     3481 tttcccgcca agcacaagcg gtcgtttttt tggaaaaatt tgcaaatgct gccaaccagt
     3541 taaagaaaaa tctccaagca ttaaaatccg aacaaggtag cgttattcac actgacaccc
     3601 tcgcctattt agcacaaaaa aataacggtg agccatttaa catcgtattt atcgatccgc
     3661 cttttcatca agggtttgta cctcaagtgc taaacttgct ggcagaaaat aactggctcg
     3721 ccccgaatgc gattttgtat gtggaaaccg agaaaaatca cccgccatta gagctaccgc
     3781 aaggctggca aattttaaag gaaaaagcag ccggaatggt ggtaagccgt ctgattcagc
     3841 tataatcttt ttgtttttta taactaaaaa atataaaata aaacttatct atctaacatt
     3901 ttattaacac tcaaattaga ataacgaaat gaaaaaatta acccctgatg aagccattga
     3961 tattgcctac gatattttct tagaaatggc aggcgaaaat ttagatcctg ccgatatttt
     4021 actgttcaac ctccaatttg aagagcgtgg tgcagtggaa atggtcgaaa cttctgaaga
     4081 ctgggatcaa gaaatcggga ctttaattga ccctgatgcc tttgccgaag tttgggttgg
     4141 cttagtcaat gaacaagatg aaatggacga tgtattcgcc cgtttcttaa tttcccacga
     4201 tgaagaaaac cgtgaatatc acgttgtttg gaaagaaaaa taaaaaggag aaaaaatggc
     4261 taatatcgac actaatcaac caatagaagc ctcgcttaaa tttcgtgaag caatggctca
     4321 cttatcttct gcggtgagca ttgtgacaac caatggcgta gccggcaaag cagggctgac
     4381 ggtttcgtct gtttgttccg ttaccgattc cccaccaacg ctcttatttt gcattaataa
     4441 aaacagccac gttcacgata ttatccaaca aaacggcaaa gtatgcgtta atgtgttaag
     4501 ccacgaacaa gaagacttag caaaacattt tgccgccatt ttagaaagta caatggaaga
     4561 acgctttggt tgggatattt ggaacaacgg ctttgacgat cagcctgtgt tacgcagtgc
     4621 tatttctgct ttacaaggca atattgttga tacgcacgca gtgggtacgc atactatttt
     4681 tgtggtagaa gtgagccata ttgacgtaga gccgaaccac agtttagttt attttgcccg
     4741 ccaatttaga agcgtgaaca ttgactaaaa tatatttaac agaaatttga tcaaatccga
     4801 cttttttagt cggatttttt atttcctgtt tttaaaacag tgctaaaatt ttgccgttta
     4861 tgtttgactg acaaaaggaa acaaaatgga aaatttaatt tgctacaaac aaatgccggt
     4921 gtggacaaaa gcaaccttac cacaaatgtt ccaagaaaag cacaacacca aagtcggaac
     4981 ttgggcaaaa ctcaccattc tcaaaggttc tctcaaatat tatgaattaa ccgaagaggg
     5041 agaaattatc aaggaagata tttttgatgc aaaccaccaa cgcccaatgg ttgagcctca
     5101 actttggcat aaggttgaag ctctttctga cgatttagaa tgccagttgg ctttctattg
     5161 caagccggaa gattattttg ctaaaaaata taatctgacc gctacccatt cagaagtgct
     5221 aaatgccgtc aattatacca aaggtggcaa agcgttagat ttaggttgcg ggcgaggacg
     5281 caatgcactt tatctgaatt tactgggttt tgatgtcact gctgttgatc ataatcaaga
     5341 aagcattgat tttctcaact atatgatcga aaaagaacaa gctcaaaaca ttactaccgc
     5401 tgtttatgat attaacgagg caactatcgg ctcgcaaaac ggtgaatatg acctgattat
     5461 ttccactgtt gtaatgatgt tcttaaaccg tgaacgcatt cctgccatta ttgagaatat
     5521 gcagaaaaac actaaagtcg gcggttataa cttaatcgta tgtgcaatga gtaccgatga
     5581 ctacccttgc ccgatgccgt tctcctttac cttcaaagaa ggcgagctcg ccgattatta
     5641 tcaaggttgg gaattagtca aatataacga agatttagga catttacaca aaactgatga
     5701 aaacggcaac cgcattcaac tacgttttgc gacaatgttg gcgaaaaaaa tcagctaaac
     5761 ttaaccgctt gcagggggaa ttagcttccc cctaattcat caaatccatt ctatttattt
     5821 tcactttttc attccgcaaa cgtttgcttg tgctataatg gcttcgtttt ttagtcattt
     5881 actgttgagg ttaaagtgag caacacacaa ctaagttaca aagatgccgg cgttgatatt
     5941 cacgcaggaa acgagttagt cgaacgaatc aagcctgatg ttaaacgcac ccgtcgccct
     6001 gaagtaatgg gcggattagg tggttttggt gctttatgtg ctttaccgac aaaatataaa
     6061 gagccgattt tagtttccgg taccgatggc gtcggcacaa aattacgctt agccattgac
     6121 ttaaacaaac acgatactat cgggcaagat ttggtagcga tgtgtgtgaa tgaccttgtc
     6181 gtgcaaggtg ctgaaccgct tttcttctta gattattacg ccaccggtaa attagaggtt
     6241 gatgtagcca ccaatgtcgt caaaggcatt gcagacggtt gcgtgatttc aggttgtgct
     6301 ttagtgggag gtgaaacggc tgaaatgccg ggaatgtacc acgaaggcga ttacgattta
     6361 gccggattct gtgtcggtgt ggtagaaaaa tctaaaatca ttgacggttc aacggtgaaa
     6421 accggtgatg ccttaattgc attagcgtcc agtggcccgc attccaacgg ctattcgtta
     6481 attcgtaaag tattggaagt cagccgatcc aatcctgcaa ccgatttatt ggaagacaaa
     6541 cccttaagcg aacacttatt agccccaact aaaatttatg taaaatcaat cctaaaatta
     6601 gttgaagaag ttgatgttca cgctatcgct cacttaaccg gtggcggttt ctgggaaaat
     6661 attccacgcg tattgccgga atctgccaaa gcggtgattg atgaatcgag ctggcaatgg
     6721 ccggcgatat ttagctggtt acaggaaaaa ggcaatatca gccgttatga aatgtaccgt
     6781 actttcaact gcggtgtcgg aatggtgata gccttacctg agagagatgt cgaaaaagcc
     6841 cttactcttc ttaaagagag tggtgaaaat gcgtggctta tcggtaaaat tgaagactta
     6901 ggcaccggta gcgagcaagt tgaaatccgc taatttgcaa aataaatgct aaaattgacc
     6961 gcttgtagta actgatctgc aaacggttaa atttttattg aggaatgcaa atgcaactat
     7021 tttggcctat ctactttgtc ttaatgaacc tagctgcctt atatttaatg tatagtgaca
     7081 aacaaaaagc gatcaaaaaa gaatggcgag tgccagaaat tactctcctc tctttttgcc
     7141 tcttaggtgg ggctatcggt gtttacctag gtatgaaata ctttcgccat aaaacccaac
     7201 attggtattt ccacgtcgct gttgtgcttt ctacttttgc ttggctgatt ggcttgcctt
     7261 acttttattt agctttatca tcatattaat ttat
//
LOCUS       DANLRK010000130         7273 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.130, whole genome shotgun sequence.
ACCESSION   DANLRK010000130 DANLRK010000000
VERSION     DANLRK010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7273
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.130"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            86..586
                     /locus_tag="PXA33_001958"
     CDS             86..586
                     /locus_tag="PXA33_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HDL5121266.1"
                     /translation="MQTINEIKEEIYPIFEPIIGTIRLKTYFSYYAVFKDGLMIALYQ
                     NGATYLRIAKKDIEYIRQYPKTCILCDEKIGIQSRKFHYIPQVLIDDTPKFSSLLFGI
                     IEELRTEKYKISQKRATQIRNLPNMNLKLERMLKKIGIHSIHDFTQEGYMSTFIKLIL
                     HRRLTV"
     gene            874..2268
                     /gene="fumC"
                     /locus_tag="PXA33_001959"
     CDS             874..2268
                     /gene="fumC"
                     /locus_tag="PXA33_001959"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005567406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HDL5121267.1"
                     /translation="MEFRIEKDTMGEVQVPADKYWAAQTERSRNNFKIGPEASMPAEI
                     IEAFGYLKKAAAYANHDLGVLAVEKRDLIAKACDEILANKLDDQFPLVIWQTGSGTQS
                     NMNVNEVVANRAHVLNGGKLGEKSIIHPNDDVNKSQSSNDTYPTAMHIAAYKKVVEHT
                     LPCVKRLQKTLAEKAEAFKDVVKIGRTHLMDATPLTLGQEFSAYAAQLAFGIKALENT
                     LPHLRELALGGTAVGTGLNTPQGYDVKVAEYIAKFTGLPFVTAQNKFEALATHDAIVE
                     THGALKQLAVSLYKIANDVRLLASGPRSGIGEILIPENEPGSSIMPGKVNPTQCEAMT
                     MVCAQVLGNDVTISFAGTQGHFQLNVYKPVMAYNFLQSAQLLGDACVSFDEHCAVGIE
                     PNYPRIKQQLDQSLMLVTALNTHIGYENAAKIAKTAHKNGTTLKEEAINLGLVTAEQF
                     DEWVRPEDMVGSLK"
     gene            2395..2505
                     /locus_tag="PXA33_001960"
     CDS             2395..2505
                     /locus_tag="PXA33_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005696979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDL5121268.1"
                     /translation="MKKLILIALVTTLGLSACGVKGPLYFPEQAPKHEQK"
     gene            2610..3860
                     /gene="lysA"
                     /locus_tag="PXA33_001961"
     CDS             2610..3860
                     /gene="lysA"
                     /locus_tag="PXA33_001961"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005620569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HDL5121269.1"
                     /translation="MNHFEYKNNQLFAEEVAIADIIEQYGTPAYVYSRSTLERHWHAF
                     NNAFGSHPHLVCFAVKSNSNIALLNVMARLGSGFDIVSQGELERVLAAGGEASKVVFS
                     GVAKSHSEIKRALEVGIRCFNIESVSELHRINQVAGELGKIAPISLRVNPDVDAHTHP
                     YISTGLKENKFGVSVTQAREVYRLAKTLPNVKITGMDCHIGSQLTELQPFLDATDRLI
                     ILMEQLREDGIELHHLDLGGGLGVPYNGEEPPHPTEYAKALLEKLKGYENLEIIIEPG
                     RAITANAGILVTKVEYLKQNEDRNFAIVDTGMNDMIRPALYEAYMQITEVDKTLNRDK
                     AVYDVVGPICETSDFLGKQRELAISEGDLIAIRSAGAYGAAMSSTYNSRPQAAEIMVD
                     GNQAHLIKARATFADLWRLEKLLP"
     gene            3874..4503
                     /locus_tag="PXA33_001962"
     CDS             3874..4503
                     /locus_tag="PXA33_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HDL5121270.1"
                     /translation="MKLYGLTGACSFVPHVALEWVKLRANQDYAFQAVSREFIKSAEY
                     LALNPRGNVPLLVDGDLALTQNQAIVHYLDELYPEAKLFGSKTARDKAKAARWLAFFN
                     SDVHKSFVPLFRLPSYAEGNETLTKTIRQQSAEQILEQLAFANAHLENHIFFGEEISV
                     ADAYLYIMLNWCRLLGLDFSHLSQLSAFMQRVEADQGVDNVREQEGLKG"
     gene            4574..6019
                     /gene="tldD"
                     /locus_tag="PXA33_001963"
     CDS             4574..6019
                     /gene="tldD"
                     /locus_tag="PXA33_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HDL5121271.1"
                     /translation="MLNQVSNSLLAPSGLELSHLTQVLDHFSGRHIDYADLYFQLSQD
                     ESWSLEDSIIKEGGFYIDRGFGVRAISGEKTGFAYADQITLKQLEQCAAAARSISPTS
                     GQLSVKNFKKTTACSRYPAINPLETISREQKVELLHLVDKTARMADPRVIQVNANLSA
                     VYEEMLVAATDGTLAADIRPLVRLSVSVLVEQNGKRERGSAGAGGRFGLDWFLAPSET
                     GFSRAESLTKEAVRQALVNLTAIPAPAGTMPIVLGAGWPGILLHEAVGHGLEGDFNRK
                     ESSLFSGKIGQMVTSELCTIVDDGTVPNMRGSITVDDEGVPSQRNVLIENGILKGYMQ
                     DKLNARLMGVAPTGNGRRESYAHLPMPRMTNTYLTEGNHEFEEMIESVEYGLYAPHFS
                     GGQVDITSGKFVFSTAEAYLIEKGKITKPVTGATLIGSGIEAMQQVSMVGKKMELDPG
                     IGTCGKEGQSVPVGVGQPTVKLDKITVGGRG"
     gene            6083..6964
                     /gene="prmA"
                     /locus_tag="PXA33_001964"
     CDS             6083..6964
                     /gene="prmA"
                     /locus_tag="PXA33_001964"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HDL5121272.1"
                     /translation="MAWVQIRLNSTDKQAEEISDFLTEIGAVSVTFMDSQDTPIFEPL
                     PGETRLWGNTDVVGLFDAETDMNLIVEALKTSKLVDENFAHKIEQIEDKDWEREWMDN
                     FHPMQFGKRLWICPSWREVPDPNAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLEGK
                     TVIDFGCGSGILAIAALKLGAKQAIGIDIDPQAILASQNNAEANGVAEKLQLFLAKDQ
                     PRELQADVVVANILAGPLKELAPNIITLVKPNGNLGLSGILATQAESVCEAYSEAFTL
                     DPVVEKEEWCRITGRKI"
     gene            complement(7139..7214)
                     /locus_tag="PXA33_001965"
     tRNA            complement(7139..7214)
                     /locus_tag="PXA33_001965"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7179..7181),aa:Trp,seq:cca)
BASE COUNT         2183 a         1607 c         1604 g         1879 t
ORIGIN      
        1 aaatctaaaa aatcctagtt gaaatttaat ccataagagt atttattatt aaataagttt
       61 catgacctta catcggagta catatatgca aacaatcaat gaaattaaag aagaaatcta
      121 ccctatattt gagcctatca ttggaacaat tcggctaaaa acctactttt cctactacgc
      181 agtttttaag gatggattga tgattgcatt gtatcaaaat ggagcaactt atttacgtat
      241 tgcgaaaaaa gatattgaat acattcgtca gtatcctaaa acctgtatct tatgcgatga
      301 aaaaattggt atacaaagta gaaaatttca ttatattccg caagttctca tagatgatac
      361 accaaaattt agctcgctac tctttggaat tatagaggaa ctccgaacgg agaagtacaa
      421 aataagccaa aagcgagcta cacaaatccg aaacctacca aatatgaatt taaagttaga
      481 aagaatgttg aaaaaaatag ggattcattc cattcatgat tttactcaag aagggtatat
      541 gtcaactttt atcaaactga ttctccaccg aagacttact gtttaagcta aacggagctt
      601 taaatcacca atatatttac accttcacag atcaacaaaa aagggaacta atgcaagaag
      661 caaatcaagc gctatataat tcaggcctaa gaaaaagatt taacgccctg tagttaaata
      721 atgtgatcca aatcataaaa tataaaaaaa ccaataagga attgcaattc gcagttcctt
      781 tctttttctt ttatatgcta gtatcacctt atacctctaa attattagtg cctactgatt
      841 ttcaattaat ccaacaattt aggagagcgc atcatggaat tccgtatcga aaaagacaca
      901 atgggcgaag tgcaagttcc tgccgataaa tactgggcgg cgcaaaccga gcgttcacgc
      961 aataacttca aaatcgggcc ggaggcatca atgccggcgg aaattatcga ggcgtttggc
     1021 tatctgaaaa aagcggctgc ctatgctaat cacgatttag gcgtactagc ggtcgaaaaa
     1081 cgggatttaa ttgcaaaagc ctgtgatgaa attttggcga acaaactcga tgatcaattc
     1141 ccgctggtga tttggcaaac cggctcgggt acgcagtcaa atatgaacgt gaatgaagtc
     1201 gtggcaaacc gtgcacacgt gttaaacggc ggcaagctgg gtgaaaaatc gattattcac
     1261 ccgaatgatg atgtgaataa atcccaatct tctaacgaca cctacccaac tgcaatgcac
     1321 atcgctgctt acaaaaaagt ggtggaacac accctacctt gtgtgaaacg tctgcaaaaa
     1381 accttagcgg aaaaagccga agcctttaaa gatgtggtca aaatagggcg tactcactta
     1441 atggatgcta ccccgctcac gctcggtcaa gagttctctg cctatgcagc acagttagca
     1501 ttcggcatca aagcattgga aaacaccctg ccacacctgc gtgagctggc actcggcggc
     1561 acagcagtcg gcaccggctt gaataccccg caaggttacg atgtgaaagt ggcggaatac
     1621 attgcgaaat tcaccggttt accgttcgtt accgcccaaa acaaatttga ggcattagcg
     1681 actcacgatg cgattgtcga aacccacggt gcattaaaac agcttgcggt gtcgctctac
     1741 aaaattgcca acgatgtccg tttattagcc tccggtcctc gttcaggcat tggcgaaatt
     1801 ctgattccgg aaaacgagcc gggttcatca attatgccgg gtaaagtaaa cccaacccaa
     1861 tgcgaagcaa tgacaatggt atgtgcccaa gtattaggca acgatgtaac catctctttt
     1921 gccggcacgc aagggcattt ccaactgaat gtctataaac cggtgatggc gtacaacttc
     1981 ctgcaatcgg ctcaactgct aggcgatgcg tgcgtgtcgt ttgacgaaca ctgtgcagtc
     2041 ggcattgaac cgaactaccc tcgcattaaa caacagctcg accaatcgct gatgttagtg
     2101 accgcattaa atactcacat tggttatgaa aatgcagcga aaatcgccaa aactgcccac
     2161 aaaaacggca caacactgaa agaagaggcg attaacttag gcttagtcac ggcagaacag
     2221 ttcgatgaat gggttcgtcc ggaagatatg gttggcagct tgaaataagc ccaacccttc
     2281 tcttgtaatc tgatttacac ctcaatctac cggtaagatt gaggtgtaat tttttatagc
     2341 gagggtactg attttgtttt agaatgaagc atcatttttt aagcggaaaa acctatgaaa
     2401 aaactcattt taatcgcatt agttactact ttaggcttaa gtgcttgtgg ggtcaaaggc
     2461 ccactctact tccctgagca agcccctaaa catgaacaaa aataagcatt gcaagcggtc
     2521 tgttttggct aaaattttgc aaatttttgt gaaaaaatga ccgcttgttg cctcttttct
     2581 ttcaaaatcc caaacacaac ggactttcaa tgaaccattt tgaatataaa aacaaccaac
     2641 tttttgctga agaagtcgct attgccgata ttatcgagca atacggcacg ccagcttatg
     2701 tttactccag atctaccctt gaacgccact ggcacgcatt caataatgcg tttggctcgc
     2761 acccacattt agtttgcttt gcagtcaaat ccaactctaa cattgccttg ttaaatgtaa
     2821 tggctcgtct cggttcaggc tttgatattg tctctcaagg cgagttggag cgtgtcttag
     2881 ctgccggcgg cgaagcctct aaagttgtct tttccggtgt ggcaaaatcg cacagcgaaa
     2941 tcaaacgagc attggaagtc gggattcgtt gctttaacat tgaatcggtt tccgaacttc
     3001 accgcattaa ccaagttgcc ggtgaactag gcaaaatcgc accgatttca ctgcgggtaa
     3061 acccggatgt ggacgcccac actcacccat acatttccac cggtttaaaa gaaaacaaat
     3121 tcggcgtgag cgttactcaa gcccgtgaag tgtatcgctt agccaaaacc ttaccgaatg
     3181 tgaaaattac cggtatggat tgtcacatcg gttcgcaatt aaccgaattg caaccttttt
     3241 tagatgcaac ggatcgctta atcatcttaa tggagcaact gcgtgaagac ggcattgagc
     3301 tacaccactt agacttaggt ggcggtttag gcgtgcctta caacggcgaa gagcctccgc
     3361 atccgaccga atatgccaaa gccttgctgg aaaagctcaa aggctatgaa aacttagaaa
     3421 tcattattga accggggcgt gccattaccg caaatgccgg tattttagtg acgaaggtgg
     3481 aatacttgaa acaaaatgaa gatcgtaatt ttgcgattgt cgataccggt atgaacgata
     3541 tgattcgccc ggctctgtat gaagcctata tgcaaattac ggaagtggat aaaaccttaa
     3601 accgtgacaa agctgtttac gatgtggtcg gtccgatttg cgaaacctcg gatttcttag
     3661 gcaaacaaag agaattggca atttctgaag gggatttaat cgctattcgt tctgccggtg
     3721 cttatggggc ggcgatgtct tcaacctaca actctcgccc acaagcggca gaaattatgg
     3781 ttgatggcaa ccaagcccat ttaattaaag cccgagccac ttttgctgat ttatggcgtt
     3841 tagaaaaatt attaccttaa tgaggaagaa aagatgaaat tatatggatt aaccggtgct
     3901 tgttcatttg taccacacgt tgccctagag tgggtgaaat tgcgtgccaa tcaagattat
     3961 gcgtttcaag ctgttagccg tgaatttatt aaatcggcgg aatatctggc tttaaaccct
     4021 cgtggtaacg taccattgtt ggttgatggc gacttggccc tcacgcaaaa ccaagctatc
     4081 gtccactatt tagacgagct ttatccggaa gctaaattat tcggcagcaa aaccgctcgt
     4141 gataaagcca aagcggctcg ttggttagct tttttcaatt cagatgtgca taaatcattc
     4201 gtgccactgt tccgcttgcc aagttatgcg gaaggcaatg aaacgctcac caaaactatt
     4261 cgccaacaat cggctgagca aattttagaa caactggctt ttgcgaatgc tcatttagaa
     4321 aatcatattt tctttggcga agaaatttcg gttgccgatg cgtatctcta cattatgctc
     4381 aactggtgtc gcctactagg gttggatttc tctcatcttt cccaactttc tgcctttatg
     4441 caacgagtgg aggcagacca aggcgtagat aatgtgcgtg agcaagaagg cttaaaaggt
     4501 taattcacca ttgttcattt aagacggtag attttatgtt aaaatctacc gtcttttatt
     4561 tttaggaatc accatgctaa atcaggtttc aaacagcttg cttgctccaa gtgggcttga
     4621 attatcgcac ttaactcaag tgttagatca cttctccggc cgtcatattg attatgccga
     4681 tctctacttc caactcagcc aagacgaaag ctggagcttg gaagacagca tcatcaaaga
     4741 aggcggtttt tatatcgacc gagggttcgg tgtgcgagcc atatcaggcg aaaaaaccgg
     4801 ctttgcctac gccgatcaaa tcacgttaaa acagcttgag caatgtgccg ctgccgcacg
     4861 ttcaatcagc ccaacaagcg gtcaattatc ggtaaaaaat ttcaaaaaaa cgaccgcttg
     4921 ttctcgctac ccggcaatca acccgctaga gaccatcagc cgtgaacaaa aggtcgaatt
     4981 gctccatttg gtggataaaa ccgctcgaat ggcagatccg agagtgattc aagtgaatgc
     5041 gaatctctca gcggtatatg aagaaatgtt agtggcagcg actgacggca cactggctgc
     5101 ggatattcgc ccgttagtgc gtttatctgt gtcggtactg gtggaacaaa atggcaagcg
     5161 tgaacgaggt tcagcaggtg ccggtggtcg ctttggttta gattggttct tagcaccaag
     5221 cgaaaccggc tttagccgtg cggaatcgct taccaaagaa gccgttcgcc aagccctcgt
     5281 taatctcaca gccattcctg caccggcagg cacgatgccg attgttctcg gtgctggctg
     5341 gccgggcatt ttgctccacg aagcagtcgg acacggcttg gagggtgatt ttaaccgtaa
     5401 ggaatcttcg ctcttcagtg gtaaaatcgg gcaaatggtc acctctgagc tttgcaccat
     5461 tgttgatgac ggtacggtgc cgaatatgcg aggctcaatc acggtagatg acgaaggcgt
     5521 gccttcgcaa cgtaatgtgt taattgaaaa cggcattcta aaaggctata tgcaagacaa
     5581 actcaatgcc cgcctgatgg gagttgcccc aaccggcaac ggcagacggg aatcttacgc
     5641 tcatttgccg atgccacgaa tgaccaacac ctacctcacc gaaggcaatc acgaatttga
     5701 agagatgatt gaatcggtcg aatacggttt atatgctcct cacttcagtg gtggacaagt
     5761 ggatattacc tccggcaaat ttgtgttctc caccgccgaa gcctacttaa tcgaaaaagg
     5821 caaaatcacc aaacctgtta ccggtgcaac cctgatcggc agcggcatag aagcaatgca
     5881 acaagtttca atggtcggca aaaaaatgga actcgacccc ggcatcggca cttgtggcaa
     5941 agaagggcaa tccgtaccag tcggcgtagg gcagcctaca gtgaagttgg ataaaattac
     6001 ggtgggggga agagggtaat ctctccccgt ttgcaaaata taaagaaaat atcaccgctt
     6061 gtagcgataa aaggaaaaca aaatggcttg ggtacaaatt cgtttaaaca gtacagataa
     6121 acaggctgag gaaatcagcg attttttaac cgagattgga gcggtgtcag tgacttttat
     6181 ggatagtcaa gataccccaa tttttgaacc tcttccgggg gaaacccgtc tttggggaaa
     6241 taccgatgtg gttgggcttt tcgatgcgga aactgatatg aatcttatcg tagaagcact
     6301 aaaaaccagc aaattggtgg acgaaaactt tgctcacaaa atcgagcaaa tcgaagacaa
     6361 agactgggaa cgggagtgga tggataattt ccacccaatg cagtttggca aacggttatg
     6421 gatctgccca agctggcgtg aagtaccaga cccgaatgca gtgaatgtta tgttagaccc
     6481 tggtttagca tttggcaccg gcacacaccc aacgacctcg ctttgcttac aatggttaga
     6541 tagcttagat ttagaaggca aaacggttat cgacttcggc tgtggctccg gtattttagc
     6601 tatcgctgca cttaaactcg gtgcaaaaca agccatcggg attgatatcg acccacaggc
     6661 gattttagct agtcaaaaca atgctgaggc gaacggcgta gcagaaaaac tacaactatt
     6721 cttagccaaa gatcagccaa gagagttaca agccgatgtc gttgttgcga atattctagc
     6781 cggcccactc aaagagttag caccgaatat catcacgctg gttaaaccaa acggcaattt
     6841 aggcttatcc ggcattcttg ctactcaggc tgaaagtgtg tgtgaggctt atagcgaagc
     6901 ctttacgcta gaccctgttg tagaaaaaga agaatggtgt cgtattacag gccgcaaaat
     6961 ctaatataag aagccctgtt ttaaacaggg cttacttttt gataaattct actactcttt
     7021 acctgtcttg ggaatcgttc tccctaaatt aattaacaca agcggtagaa ataacaggaa
     7081 aatttacaaa ttacataaat gaaaaaagcc gactgaaatc agtcggcttt ctaaataatg
     7141 gcaggggcgg agaggctcga actcccaaca cccggttttg gagaccggtg ctctaccaat
     7201 tgaactacgc ccctattgat aggtattcat tttgcactta taggggcgaa ttactattcg
     7261 cccctgcatt aaa
//
LOCUS       DANLRK010000131         7235 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.131, whole genome shotgun sequence.
ACCESSION   DANLRK010000131 DANLRK010000000
VERSION     DANLRK010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7235
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.131"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            75..980
                     /locus_tag="PXA33_001966"
     CDS             75..980
                     /locus_tag="PXA33_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDL5121273.1"
                     /translation="MSQKYSVAVIGLGAMGMGAAKSCIAAGLDTYGVDLNPNYLETLK
                     AAGAKKVGTSAVDFAAELDAVLLLVVNANQVNAVLFGENGLAQHLKQGTVVMVSSTIS
                     AQDAKNISQKLTDLGLVMLDAPVSGGAAKAAQGEMTVMASGSSEAFEKLKPVLDAVAG
                     KVYNIGEEVGLGATIKIIHQLLAGVHIAAGAEAMALAARAGIPLDLMYDVVTNAAGNS
                     WMFENRMKHVVEGDYTPLSMVDIFVKDLGLVNDTAKSLRFPLHLASTAYSMFTEASNA
                     GYGKEDDSAVIKIFSGIELPKKEGK"
     gene            982..2223
                     /locus_tag="PXA33_001967"
     CDS             982..2223
                     /locus_tag="PXA33_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005754893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HDL5121274.1"
                     /translation="MLGIIADDFTGASDIASFLTENGLKTVQMNGVPKTTLNSQVDAI
                     VISLKSRSNPVEEAIQQSLQALDWLKQNGCSQFYFKYCSTFDSTEKGNIGPVTDALMA
                     ALDTDFTVVTPALPVNGRTIFNGYLFVGDVLLNESGMRNHPITPMKDANLVRLMDAQS
                     QGKTGLVAYADVIQGAERVKARFAELKQQGYKYAVVDAADNSQLAVLAEAVADFQLVT
                     GGSGLGAYMAAYRSGGKKGTNAFVPSKGKTVVLSGSCSVMTNTQVAAYQQKAPSYFLE
                     AEKAIQDPNYVETLYQWVIANLDGDFAPMVYATVPPEKLKEIQTQFGGDKASHAIENT
                     FAKLAEKLQAAGVTNFITAGGETSSIVVQQLGFSGFHIGKQIAPGVPWLQAVEKPIFL
                     ALKSGNFGKEDFFSFAQEMFV"
     gene            2220..2852
                     /locus_tag="PXA33_001968"
     CDS             2220..2852
                     /locus_tag="PXA33_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014668373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HDL5121275.1"
                     /translation="MTEQEQKIEMVNLARSLFERGYTVGGAGNLSVRLDENRILVTPT
                     GSSLGRLEADRLSVLDMQGNVLSGDKPSKESVFHLAMYQKNPACQAIVHLHSTYLTAL
                     SCLENLDTDNAMRAFTPYYVMRVGKLPVIPYYRPGDINIARELGERALDGKAFLLANH
                     GVVVTGSDLRDAVDNTEELEETSKLYFILQGQKVRYLTDDEVNDLKNRGK"
     gene            2854..3630
                     /locus_tag="PXA33_001969"
     CDS             2854..3630
                     /locus_tag="PXA33_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005547735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase family protein"
                     /protein_id="HDL5121276.1"
                     /translation="MPKFAANLTMMFNEVPFLDRFEAAANAGFKYVEYLWPYDYPAAE
                     LKAKLEQYGLKQVLFNTAAGDVAQGEWGVSAIPGREADSHRDIDLALEYALALGCENV
                     HVMAAVVPEGEDREAYKQTFIKNVQYAADKFKPHGINVLLEALSPEVKPNYLLKSQFD
                     TLEIVEAVNRDNVFVQLDYFHAQNVDGNLSRLTDKLKGKFAHIQIASVPDRHEPDEGE
                     INYQYIFNKLDEIGYEGYVGCEYKPRAETTSGLGWFEQYK"
     gene            3683..5041
                     /locus_tag="PXA33_001970"
     CDS             3683..5041
                     /locus_tag="PXA33_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntT protein"
                     /protein_id="HDL5121277.1"
                     /translation="MSNEMLILIGLASVVALLVIMIKGKVHPFLALGLVSIAVALTAG
                     IPMSKVIPTLISGMGSTLGSVALIVGLGAMLGKIIEKSHGADVLANWLLQRFGEKNAP
                     FALGMTGFIFGIPVFVDVGFIVLLPIIFSVARRLGGNILVYSLPIGLAMLLVHVLVPP
                     HPGIVAGAQNLNADIGFVLGIGLVAALPAYLIGQKFIPFFTKNNFVKVVTSTDLLQHQ
                     KDLIKEKGIELPSFGKVLAMISFPLILIMLGTVTATTLPKEHIVREVFSMVGSSPFAL
                     FVAVCLSAYVLGFRYGWTKHKLEEVLESALAPIAGIILITGAGGMFGKVLDASGVGKA
                     LADVLANTGLPLLVLSFILSALLRAAQGSATVATITTTTILAPAAVAAGYNDLQLALL
                     TAAIGAGSMTLSHVNDSLFWVWTKFFNVPVSVGLRTWTFLTTIFGVIAFAVVGIIWML
                     FV"
     gene            5103..6443
                     /locus_tag="PXA33_001971"
     CDS             5103..6443
                     /locus_tag="PXA33_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HDL5121278.1"
                     /translation="MLKKTLLFTLLLSHSLYAEDMIQENKSANITLLAEITAQGQKIN
                     GVALEYPDNVLSGSDLRQLYQVKTALDSAEKENRSVLKAYVNNEPKVTALPQAGKFVI
                     IELDTADKNAATYSVKDANKAPMKFKSKDENGNIVETEKVQAVKIPEFFANRLIYSVE
                     QKGLLKLTSGTTLGKTEWTQTAEPDKIKTPYLDDFVAKQVSLDQSGNKLNYRLYSPLQ
                     KNGEKYPLVIFLHGSGQVGSDNLAHLLSSKGAIATLPYAPSFVLAPQYDQVFDPFDNA
                     AKGQKGGIHWQTENRINLVLKMIDETLKANPQIDTTRIYINGLSRGAEGGLNLLLARP
                     NFFAGALLMSGREAYTNEWVDGNATKTNLAAIKHIPIWFFHSKEDKVSPVKGSRINYR
                     ILKDELNAPNVKYTEFTFEQAGDNGIVNNNPHNTWDAVYNSPEVIGWLLQQKRH"
     gene            6455..7144
                     /locus_tag="PXA33_001972"
     CDS             6455..7144
                     /locus_tag="PXA33_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005577085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HDL5121279.1"
                     /translation="MFMYLSHPLDPKDLAWPGEPVVEVTRCTDVGEETPFSSFITKLP
                     NHCGTHMDAPRHFVKDGLSINELPIEYFCHKEVALIEIPKGEAEGITKEDLLPYVDIL
                     SKVSFAFLRTGFEKYRTENPAIYQNECPYISTGAGQYLSDTFPNLKGVGIDFLALGSP
                     SSRVPDAENPKNCHRAILGYYTGRFTTVIEDMHLAELPKNANIKQFFNAPWRIKGLDS
                     SQVTCIVELQQ"
BASE COUNT         2089 a         1469 c         1660 g         2017 t
ORIGIN      
        1 catcacagat tttatctttt ctattggtta atataccgcc atcaagttca ttaatttatt
       61 tgagggaata aacaatgagt caaaaatata gcgtagccgt catcggctta ggagcaatgg
      121 gtatgggagc agcaaaatct tgtatcgctg ccggattaga cacatacggt gtggatctta
      181 acccgaatta tctggaaacc ttaaaagcag caggtgctaa gaaagtcggc acaagtgcgg
      241 tcgattttgc cgctgaatta gatgcggtgt tgttgttggt ggttaatgcc aaccaagtga
      301 atgcggtgtt attcggtgaa aacggcttag cccaacattt gaaacaaggt acggtggtga
      361 tggtatcttc caccatttct gcacaagatg caaaaaatat ttcacagaag ttaacagatt
      421 taggcttggt gatgttagat gctccggttt cgggtggggc agcaaaagca gcacaaggtg
      481 aaatgactgt gatggcatca ggttccagcg aggcattcga gaaattaaaa ccggtattag
      541 atgcggttgc aggcaaagtg tataacattg gcgaagaagt cggcttgggt gcaacgataa
      601 aaattatcca tcaattactt gccggtgtgc atattgccgc aggtgcagaa gcaatggcat
      661 tggcggctcg tgccggtatt ccgcttgatt taatgtatga cgttgtaacc aatgcggcag
      721 gcaattcttg gatgtttgaa aaccgaatga aacacgttgt tgagggcgat tacaccccac
      781 tctctatggt ggatattttt gtgaaagatt tgggattggt gaacgacacc gcaaaatctt
      841 tgcgtttccc gcttcactta gccagcaccg cttattctat gtttaccgaa gccagcaacg
      901 caggttacgg caaggaagat gatagtgcgg tcattaaaat tttcagtggt atcgaattac
      961 ctaaaaaaga ggggaaataa gatgttaggc attattgcag acgatttcac cggtgcgagt
     1021 gacatcgcca gtttcttaac cgaaaacggg ctaaaaacgg tgcaaatgaa tggtgtgccg
     1081 aaaactacac ttaacagtca agttgatgcg attgtgatta gcctgaaatc tcgctctaac
     1141 cctgtggaag aagcaatcca acaatcatta caagccttag attggctgaa acaaaatggt
     1201 tgctcgcaat tctactttaa atattgttcc acttttgaca gcacggagaa aggcaatatc
     1261 gggccggtaa ccgatgccct aatggcggca ttagataccg attttaccgt agtcacaccg
     1321 gctctgcctg tcaatggcag aaccattttc aacggctatt tgtttgtggg cgatgtgcta
     1381 ctgaatgaat ctggtatgcg taaccacccg attaccccga tgaaagatgc caacttggtg
     1441 cgtttaatgg atgcacaatc tcaaggcaaa accggcttgg ttgcctatgc tgatgtgatt
     1501 caaggggcag agcgtgtgaa agcccgtttt gctgaattga aacagcaagg ctacaaatat
     1561 gcggtagtag atgctgccga taattcacaa cttgcggtgc ttgccgaagc ggttgccgat
     1621 tttcaattag tcacaggcgg ttcaggctta ggggcttata tggcggctta ccgttcaggc
     1681 ggcaaaaaag gcacgaatgc gtttgtacca agcaaaggca aaaccgttgt gctttcgggt
     1741 tcttgctcgg taatgaccaa tacccaagtg gcggcttacc agcaaaaagc cccaagttat
     1801 ttcttagaag cggaaaaagc cattcaggat ccgaactatg tagaaacgct ttatcaatgg
     1861 gtgattgcaa acctagacgg tgactttgcc ccaatggttt atgccaccgt tccccctgaa
     1921 aaattgaaag agattcaaac ccaattcggc ggcgataaag ccagccacgc cattgaaaat
     1981 acctttgcca aattagccga aaaattacaa gccgcaggcg taactaactt tattaccgca
     2041 ggcggtgaaa cttcgagcat tgtggttcag caacttggat tcagcggttt ccacatcggc
     2101 aaacaaattg caccgggcgt gccgtggtta caagcggtcg aaaaaccgat ttttcttgca
     2161 ttgaaatcgg gtaacttcgg taaagaagat ttcttcagtt ttgctcagga gatgtttgta
     2221 tgacagaaca agaacaaaag attgaaatgg tcaatcttgc ccgctcgctg tttgagcggg
     2281 gctataccgt tggcggtgcg ggcaacctct ccgtgcgttt agatgaaaat cgcattttag
     2341 ttaccccaac cggctcttcg ctcgggcgtt tagaggcaga ccgcctttcc gtattggata
     2401 tgcagggtaa tgttctttca ggagataaac cttcaaaaga atcggtattc cacttggcga
     2461 tgtatcagaa aaatccggct tgccaagcaa tagtgcatct gcattccact tatttaaccg
     2521 cactttcttg cttggaaaat ttggataccg acaacgctat gcgtgctttc accccttact
     2581 atgtgatgcg ggtaggaaaa ttgccggtca ttccttacta ccgtccgggc gacatcaaca
     2641 ttgcccgaga gctgggggaa cgagccttag acggcaaagc ctttttactg gcaaatcacg
     2701 gggtggtagt cacaggttca gacttgcgtg atgcggtgga taacacagaa gagttggaag
     2761 aaacctccaa gctctatttt attctacaag gtcaaaaagt ccgttatctc accgatgacg
     2821 aagtcaatga tctgaaaaac agaggaaaat aaaatgccta aatttgcagc aaaccttacg
     2881 atgatgttta acgaagtgcc gtttttagat cgttttgaag cggctgccaa cgctggattt
     2941 aaatatgtcg agtatttatg gccttatgat tatccggcag cagaattgaa agccaaactt
     3001 gagcaatatg gcttaaaaca ggtgttgttc aataccgcag ccggagatgt tgctcaaggt
     3061 gaatggggtg tttctgccat tccggggcgt gaagctgata gccatcgtga tattgattta
     3121 gcgttggaat atgccctcgc cttaggttgt gagaatgttc acgttatggc agcggtcgtg
     3181 ccagaaggcg aagatcgtga agcctataaa caaaccttta tcaaaaacgt gcaatatgcc
     3241 gccgataaat tcaaaccgca cggcattaac gtcttgcttg aagccctaag ccctgaagtg
     3301 aaaccgaatt atctgcttaa aagccagttt gatacgctag aaattgtgga agcggtaaac
     3361 cgagacaatg tgtttgtaca gcttgactac ttccacgcac agaatgtgga cggcaactta
     3421 tctcgcctaa ccgacaaact caagggtaaa tttgcccaca ttcaaattgc ctctgtgcca
     3481 gatcgccacg aaccggacga aggggaaatt aactaccaat atatcttcaa caaactcgat
     3541 gaaatcgggt atgaaggcta tgtaggttgc gagtataaac cacgagcgga aaccacctca
     3601 ggactaggtt ggtttgagca gtataaataa ccacgatgat tagcggataa atacgtgaat
     3661 acaacattta gaaggaaact ttatgtcaaa tgaaatgtta attttgattg gattagcaag
     3721 cgtagttgct cttctggtca ttatgattaa aggtaaagta cacccattct tagcattggg
     3781 gttagtgagt atcgcagttg ccttaactgc aggtattcca atgagtaaag tgatcccaac
     3841 cttgatttca ggtatgggaa gtactttagg tagcgttgca ctcatagtag gtttgggagc
     3901 aatgttaggg aaaatcattg aaaaatctca cggtgcggat gtattagcaa attggttgtt
     3961 acagcgtttt ggtgagaaaa acgctccctt tgcattaggt atgacaggtt ttatattcgg
     4021 tattcctgtt ttcgttgatg ttggttttat cgtattgctc ccgattattt ttagtgtagc
     4081 gagacgttta gggggaaata tccttgttta ttctttgcca atcgggcttg cgatgttact
     4141 tgttcacgtt ttagttccac ctcatccagg tattgtagcg ggtgctcaaa acttaaacgc
     4201 agatattggc tttgtattgg gaattggctt agtagcagcg ctacctgcat atttaatcgg
     4261 tcaaaaattt attccgttct ttacaaaaaa taatttcgta aaagtagtaa cttcaaccga
     4321 tcttttacag caccaaaaag atttgattaa agagaaaggc attgaactac cttcttttgg
     4381 taaagtgtta gcaatgattt ctttcccgct aatcctgatt atgttaggta cagtaacggc
     4441 aacaaccttg ccgaaagaac atatcgtgcg tgaagtattc agtatggtag gaagctctcc
     4501 gtttgcctta tttgtggctg tttgtctttc tgcttatgta ttaggtttta gatacggttg
     4561 gactaagcat aaattagaag aagtactgga gtctgcgtta gcaccgattg cggggattat
     4621 tttaatcacc ggtgcaggcg gtatgttcgg taaagtgtta gatgccagtg gcgtaggtaa
     4681 agcattagca gatgtattag ccaacacggg cttaccgtta ttggttcttt ctttcatact
     4741 ttctgcatta ttgcgtgcag cacaaggttc tgcaacggtg gcaactatta ctactacaac
     4801 tattctagct cctgcagctg tagcagcagg ttataatgat ctgcaattag cacttcttac
     4861 tgcagctatt ggtgcaggtt ctatgacatt atcccacgtg aatgattcac tcttctgggt
     4921 atggacgaaa ttcttcaatg ttcctgtatc cgtaggctta agaacttgga cgtttttaac
     4981 gactattttc ggtgtaatag cctttgccgt tgttggcatt atttggatgt tattcgttta
     5041 atatcactct gttactgctt aaaatccacc tcttatgagg tggatttttt taggagaaac
     5101 ctatgctgaa aaaaacactt ttatttacgc tattactttc tcactccctc tatgcagagg
     5161 atatgattca ggaaaataaa tcagcgaata ttacgttgct tgccgaaatc accgctcaag
     5221 gtcaaaaaat taacggcgtg gcattggaat atcctgataa tgtcttatca ggcagcgatc
     5281 ttcgccaact gtatcaggtc aaaacggcat tagatagtgc ggagaaagaa aaccgttcgg
     5341 tgctcaaagc ctatgtcaat aatgagccaa aagtgaccgc acttccgcaa gccggtaaat
     5401 ttgtgattat tgaattggat acggcagata aaaatgcggc aacttatagc gtaaaagatg
     5461 ctaataaagc gccgatgaaa tttaaatcca aagatgaaaa cggcaacatt gtagaaacag
     5521 agaaagtaca agcggtcaaa attccagagt tttttgcaaa tcgcctaatc tattcggtgg
     5581 aacaaaaagg cttgcttaaa ttaaccagcg gcacaactct aggcaagact gaatggacac
     5641 aaaccgctga acccgacaaa atcaaaaccc cttatctcga tgattttgtc gcaaaacaag
     5701 tgagcttaga ccagtctgga aataaactga actaccgtct ctattcgcct ttgcaaaaaa
     5761 atggtgaaaa atacccgctt gtgattttcc tacacggttc agggcaagtc ggttcggata
     5821 accttgctca cctgctttcc agtaaagggg cgattgccac cctgccttat gccccaagtt
     5881 ttgtacttgc tccgcaatac gaccaagtat tcgacccatt tgataatgcc gccaaagggc
     5941 aaaaaggcgg tattcactgg caaacggaaa atcgtatcaa tctcgtgtta aaaatgattg
     6001 atgaaaccct aaaagccaat cctcaaattg ataccacacg catttatatc aatggcttat
     6061 ctcgaggtgc tgaagggggc ttaaatctgt tgttggctcg cccgaatttc tttgccggag
     6121 cattattaat gagcggtcgt gaagcctaca ccaacgaatg ggtggacggc aatgccacca
     6181 aaacgaattt ggcggcaatt aaacatatac cgatttggtt tttccatagt aaagaggaca
     6241 aagtctcgcc ggtaaaaggt tctcgcatta actaccgcat tctcaaagat gagttaaatg
     6301 cccccaatgt gaaatatacc gaattcactt ttgaacaagc cggcgacaac ggcattgtca
     6361 ataataaccc tcataatact tgggacgctg tgtataacag ccctgaggtg attgggtggt
     6421 tacttcagca aaaacgccac taaggaggct cactgtgttt atgtatttgt ctcacccgtt
     6481 agatcctaaa gatttagctt ggccgggaga acctgttgtt gaggtgactc gctgcacaga
     6541 cgtaggtgaa gaaacgccat ttagcagttt tatcacgaaa ctaccgaatc attgcggcac
     6601 acatatggac gctcctcgcc actttgtgaa agacggttta agtatcaatg aattgccgat
     6661 agagtatttc tgccataaag aggtcgcttt aattgaaatt cccaaaggag aggcagaagg
     6721 gatcaccaaa gaagatttat tgccttatgt cgatattctc tccaaggtgt cttttgcctt
     6781 tttacgaacg gggtttgaga aatatagaac cgagaaccct gctatttatc aaaatgaatg
     6841 cccctatatt tcaaccggtg ccgggcagta tttatctgat actttcccga atttgaaagg
     6901 agtaggcatt gatttcttgg cactagggtc tccttcttcc cgagtgcctg atgcggaaaa
     6961 ccctaaaaat tgccatcgag cgattttagg ctactatacc ggcaggttta ccactgtgat
     7021 tgaagatatg cacttagcgg aactccctaa aaatgccaac attaagcagt tttttaatgc
     7081 accgtggcga attaaagggt tagattccag tcaagtgact tgtattgtgg aattacagca
     7141 ataaaccaca gatatattca ttttaggaga ggctatttac tatagtaagc ataactcaat
     7201 cattaattga aaataattat tatttgattt taatc
//
LOCUS       DANLRK010000132         7231 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.132, whole genome shotgun sequence.
ACCESSION   DANLRK010000132 DANLRK010000000
VERSION     DANLRK010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7231
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.132"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(8..1783)
                     /locus_tag="PXA33_001973"
     CDS             complement(8..1783)
                     /locus_tag="PXA33_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HDL5121280.1"
                     /translation="MKTPQNLILSTVLLSASFVAEANSSSLVQQARIDATQQQIDAAQ
                     QHRQQQYQQNQAEKFQPKADVRLETQINDTLTLSNHELPCYPIHQITLIDYSPDNSLT
                     TSQFQWAFEKAVKSFHLTLPHCFGGEGLAVLMKQIQNEIIGKGYVTTRVVTAEQDLRG
                     GKLVLTVIPGKIRNTLVVDSGKVPRFTKLHALTGFTFAEGDILNVRDIEQSLENLKRV
                     PTAEANIEILPSSSEKAEVGDSDLKISYSQAFPFRLNLGLDDAGSKSTGKLQTSATLS
                     IDNIFSANDLFYTSFTHSMKQKGDDNGRRASKNLTLYYSVPFGYWQLAFSHTHHRYHQ
                     EVFGAFDKSYLYAGESDTDKLTFSYLLYRDAKRKTSLSGSLWPRQSQNYIDGSEIEVQ
                     KRRMAGWEAGFSHKEYFPTATLELSANFKRGTAARGALSAPEEDYGEGTSRPKIITAS
                     ASLTKPFFWGKQPWQWQSSLNAQWNKTPLVPQDRFSIGGRYTIRGFDGELTLSGERGW
                     NWRNELSWQFNPNHAFYWAVDGGRVSGWATENQLGHHLMGTAIGFRGGVKGFSYDVFA
                     GRPMRKPEGFKTSDTVAGFNIGYNF"
     gene            1938..3023
                     /gene="queA"
                     /locus_tag="PXA33_001974"
     CDS             1938..3023
                     /gene="queA"
                     /locus_tag="PXA33_001974"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HDL5121281.1"
                     /translation="MLVSDFHFNLPDELIARYPTAERSASRLLHLNGETGEFADQQFV
                     DLYDHINEGDLLIFNNTRVIPARLYGRKPSGGKVEALVERVLDEHRCLAHVKASKAPK
                     EGAELIFGEDKLGEGKGFTATMVARHDTLFELVFNEEQPLFELLQQAGHMPLPPYIDR
                     PDEDADQERYQTVYSKVLGAVAAPTAGLHFDNAMLEKLKAKGVDTAFVTLHVGAGTFQ
                     PVRVETIEDHKMHAEYAEVSQEVVDKILATKAKGKRVIAIGTTSVRSVESAAQAAEKD
                     GKLIAPFFSDTAIFLYPGKTFKVIDALVTNFHLPESTLIMLVSAFAGYRNTMNAYAHA
                     VETKYRFFSYGDAMFINKNPNALSDVP"
     gene            complement(3050..3588)
                     /locus_tag="PXA33_001975"
                     /pseudo
     CDS             complement(3050..3588)
                     /locus_tag="PXA33_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015701733.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3601..5883)
                     /locus_tag="PXA33_001976"
     CDS             complement(3601..5883)
                     /locus_tag="PXA33_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HDL5121282.1"
                     /translation="MTLDRFCIALILFHLPILWLPKAFLFVGIVIGTLVLILSVYLRS
                     KLTAVLGLLVCIGYIQIIQVAKNAEKTTASKTPVQFEISKILKQTDYQTAIATLNTGE
                     KIYLNWQSKQPLLLNQFYQAELALRPISARSNIGNFDRQRWYFANHINMTATVRKVET
                     LAKTDYSLRTNWLNKVKAETDALPTQGFLLALAFGERAWLKTEHWGQFQQTATAHLIA
                     ISGLHIGLAMLFGVYFAKGVQWLCLKSELTALQAVGFSLYFARIVSFITAFGYSYLAG
                     FAVPTARALLAIAFVLLCQVLRRHYTPWQYWGRIVALLILIDPLSLLSDSFWLSILAV
                     ASLILWYQYFPLSRFLNDEYRKKITKFNRLWLSLLHLQIGIWLIFSPVQLYFFEGISA
                     FALLANLIIVPLYSFLLVPLILFTLLTDNLFSTWLLADYLAQFSLWLLDPFSNAWFAL
                     SHWQQWQLLSLNLLVLVLLYCKLYRLSYKKWLSAVAVSLLFNLSFYLPKLFNQTQTEW
                     IMFDVGQGLAMAFIYQQNKAVIYDTGSSWQANDGSRNSMAKMEILPYLKRNGIEVEAI
                     FLSHDDNDHSGGVVELLQAYPKARLISSSKVTYHNINPEPCIAGTSWQFGEWQLLAIY
                     PSHIAERAKNQDSCVILAKNDRLKILLTGDSGVEQERQFAHQLGKIDFLQVGHHGSKS
                     STGETLLSITQPAFAVISAGRWNPWKLPNKQIIERLNRRHINVLNTVETGMIRVKFYP
                     SSIEIEQARGAFSPWYKQFY"
     gene            complement(6025..7215)
                     /gene="sstT"
                     /locus_tag="PXA33_001977"
     CDS             complement(6025..7215)
                     /gene="sstT"
                     /locus_tag="PXA33_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005610059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HDL5121283.1"
                     /translation="MLGGNLVLRIAIGLVLGVLLAFINKEWATSVGVLGQFFVKSLRA
                     IAPILVFVLVLAAIANKEVGSDSKLKPILVLYVLGTFFAALTAVVLSFMFPTTLELTA
                     SPDGLTPPQGIGEILKTVIFNLVDNPLVALSNANFIGILAWSIGLGIAFRHGSASSKA
                     FLNDLANAVSFVVKVVIAFAPIGVFGLVAETVAINGIDAFEDYARLLVVLVGAMLFVA
                     LVLNPLLVYWKIRRNPYPLTFTCLRESGVTAFFTRSSAANIPVNMNLAKRLGVRDEIA
                     SVAIPLGASINMAGAAITVTVLTLAAAYTQGIQPDFATALLLSVVASICACGASGVAG
                     GSLLLIPLACSLFNIPNDIAAQVIGVGFVIGVIQDSVETALNSSTDVLFTAAVSYAED
                     QKSA"
BASE COUNT         2201 a         1559 c         1425 g         2046 t
ORIGIN      
        1 tcattcatta aaaattgtaa ccaatattaa aaccggcaac ggtgtctgag gttttaaacc
       61 cttccggttt tcgcatcggt ctaccggcaa acacgtcata actaaatcct ttaactccac
      121 cccgaaaccc aatggctgtt cccattaaat gatgtccgag ctgattttcc gttgcccaac
      181 cggacactcg accaccatct accgcccaat aaaaagcgtg gttcggatta aattgccagc
      241 tcaactcatt acgccagttc cagccccgct caccggaaag agtcagttca ccgtcaaatc
      301 cacgtatggt gtaacgcccg ccaatcgaaa aacggtcttg cggaaccaga ggggttttat
      361 tccattgggc atttaggcta ctttgccact gccatggctg cttgccccaa aaaaacggtt
      421 tcgtcaggct tgcacttgcc gtgatgattt tagggcgaga agtcccttcg ccataatctt
      481 cctcgggagc agataatgcc cctcgtgctg ccgtacctcg tttgaaatta gccgaaagct
      541 caagcgtagc ggtcgggaaa tattctttat gcgaaaaacc cgcttcccaa cctgccattc
      601 ggcgtttttg tacttcaatt tctgagccgt caatatagtt ttgcgactgg cgtggccaaa
      661 gtgaaccgga caagctcgtt ttacgtttgg catcacgata aagcaaataa gaaaaggtta
      721 atttatcggt atcactttca ccggcgtaga gatagctctt gtcaaaggca ccaaatactt
      781 cttgatgata acgatgatga gtgtgtgaaa atgctaactg ccaatagcca aacggcacgg
      841 aatagtaaag cgtgagattt ttactggcac gtctgccgtt atcatcacct ttttgtttca
      901 tcgaatgggt aaaagaggta tagaacaggt catttgccga aaaaatatta tctatagaca
      961 gagtggcgga ggtttgtaat ttgcctgtgg atttcgagcc ggcatcgtcc agcccaagat
     1021 ttaagcgaaa tggaaacgct tgcgaatagc tgatttttaa atcactgtct cccacctccg
     1081 ctttttcgct tgagctgggt aaaatctcaa tatttgcctc cgccgtcggc acccgtttta
     1141 gattttccag agactgctca atatctcgca cattcagaat atcgccttcg gcaaaagtaa
     1201 aaccggttaa ggcgtgcagc ttggtaaaac gcggtacttt gccgctatct accactaaag
     1261 tattgcgtat tttgcccgga atcacggtta aaactaattt tccgccacgt aaatcttgct
     1321 ccgcagtaac cactcgtgta gtgacataac ctttgccaat aatttcattt tggatttgct
     1381 tcatcaacac agctaaccct tcaccaccaa agcagtgagg taatgtcaaa tgaaatgatt
     1441 ttactgcttt ttcaaacgcc cattggaatt ggctggtagt cagagaattg tcaggagaat
     1501 agtcaatgag agtgatttga tgaatggggt aacaaggcaa ttcgtgattg gaaagagtaa
     1561 gtgtatcatt gatttgtgtc tcgagccgta catcagcttt cggctggaat ttttctgctt
     1621 gattttgttg gtattgctgt tggcgatgtt gctgggcagc atcaatttgt tgttgagttg
     1681 catcaatgcg tgcttgttgt acaaggcttg aagaatttgc ttcagcaaca aaagaagcac
     1741 ttaacaatac cgtgcttaaa atcagatttt gtggggtttt cataagatca aataagtaag
     1801 acattcaacg tttttagtct ataaaaattt atgttgtttt gcaaactccg ttttatggag
     1861 ctttatacat atctgacgac tttggttttt tatgctatta tctccgccat ttttatacta
     1921 tagagaatag aataaccgtg ttagtttcag attttcattt caacttaccc gatgagctga
     1981 ttgctcgcta cccgactgcc gaacgctctg ccagccgttt attacactta aatggcgaaa
     2041 caggcgaatt tgccgatcaa caatttgtgg atttatacga tcatatcaac gaaggcgatc
     2101 tattgatctt caataacact cgagtgatcc cggctcgcct ttacggtcga aaacctagtg
     2161 gcggcaaagt agaggcatta gtggaacggg tgctagatga acaccgctgc ttggctcacg
     2221 ttaaagcctc aaaagcaccg aaagaagggg ctgaattgat ctttggggaa gataagctag
     2281 gtgaaggcaa aggctttacg gcgacaatgg tagctcgcca cgatactctg tttgaattgg
     2341 tgtttaacga agagcaaccg ttattcgaat tgctacagca agctggtcat atgcctcttc
     2401 caccttatat cgaccgcccc gatgaagatg ctgaccaaga gcgttatcaa accgtttata
     2461 gcaaagtgtt aggtgcagtt gccgccccta ccgccggttt gcattttgac aatgcaatgc
     2521 ttgaaaaact aaaagcaaaa ggggtggata ctgccttcgt aacattgcac gttggtgccg
     2581 gcactttcca gccggtacgg gtagaaacca ttgaagatca taaaatgcac gccgaatatg
     2641 ccgaagtgag ccaagaagta gtcgataaaa ttctcgcaac gaaagccaaa ggaaaaagag
     2701 tgattgcaat tggtacgaca tcagtacgtt cggttgaaag tgccgcccaa gcggctgaaa
     2761 aagatgggaa attgattgct ccgttctttt ctgacaccgc tattttcctt tacccgggaa
     2821 aaacctttaa agtcattgat gcgttagtca ccaatttcca tttaccggaa tccacgctaa
     2881 ttatgttagt ctcagcattt gccggctata gaaatacgat gaatgcctac gcccacgcag
     2941 tagaaactaa ataccgtttt ttcagctatg gcgatgcgat gtttatcaat aaaaacccaa
     3001 atgcgttgag tgatgttcct taatttatga gggttataag ggaataacat tatgaacaaa
     3061 aacaatcttc caaccatttt aagggattgt tttcaatata ttcaataatt tggaagtacg
     3121 atgtttcatt acgaataatg tgttcataat aattcctttg ccaaatttca gaaatatcgg
     3181 tattttgacg aacccattta gttacgccaa ttttaaaacc acgcacaata gagccgattg
     3241 ttcgtgatgt tccccgtaag ggcgaaacat ttttcgccct tatacatggg gcaaatgatg
     3301 atttgcccct acagtttcga cgatttcaat aatgccatgt aaatgattag gcataatcac
     3361 aaagctatgc aagcttacat ttggaaaatg atcgggtata tcttcccaac attgacgtac
     3421 aatattccct atcttattaa gcctcatttc atcatcaaca atctcaccga gaaaatggtg
     3481 tttattttta caacaaatag taatgaaata gaatccttgt gatcggtaat cataatgctt
     3541 tagacgaatt gaacgcctcc tatgaatctc cggctgatat ttctgcattt ttcttattcc
     3601 ttaataaaac tgcttatacc aaggggaaaa tgcccccctt gcctgctcaa tttcaataga
     3661 acttgggtaa aattttactc ttatcattcc cgtttctacg gtatttaata cattaatatg
     3721 tcttctgttt aatctctcaa taatctgttt attcggtaat ttccatgggt tccatcttcc
     3781 agcagaaatc actgcaaatg ctggctgggt aatcgataat aaagtttcgc cagtgctgct
     3841 tttgctaccg tgatgtccaa cctgtagaaa atcaattttg ccgagctgat gggcaaattg
     3901 gcgttcttgt tccacgccag aatcccctgt cagtaatatc ttcaagcggt cgtttttggc
     3961 taaaataacg caagagtctt ggtttttagc tcgttctgca atgtgtgacg ggtaaattgc
     4021 caataattgc cattcgccaa attgccacga tgttccggca atacaaggct cgggatttat
     4081 attgtgatac gtcaccttgc ttgaactaat taaccttgct tttgggtatg cttgcaataa
     4141 ctccactacc ccacctgaat gatcattatc atcgtgactt agaaaaatcg cctctacctc
     4201 aatgccattt cgctttaaat acggcaagat ttccattttt gccattgaat tgcgactacc
     4261 atcatttgct tgccaactgg agccggtatc gtaaattacc gctttatttt gttgataaat
     4321 gaacgccatt gccagccctt gcccaacatc aaacataatc cattctgttt gggtttgatt
     4381 aaagagctta gggagataaa aactcagatt gaacaatagg gataccgcta ctgcacttaa
     4441 ccacttttta taagatagcc gatataattt gcaatacagc aatactaaaa ccaataaatt
     4501 cagtgaaagt aactgccact gttgccaatg gcttaatgca aaccacgcat tagaaaatgg
     4561 atctaacagc cataggctaa attgagcaag atagtccgcc aacagccacg ttgaaaacag
     4621 attgtcggtc aagagcgtaa ataaaataag cggtacaagc aaaaagctat acaacggcac
     4681 aataatcagg ttggcaagca gtgcaaaagc agagatgccc tcaaaaaagt agagttgaac
     4741 cggtgagaaa atcagccaaa tgccgatttg taagtgcaaa agcgataacc acaagcggtt
     4801 aaattttgtg atttttttac gatattcatc gtttaaaaat cggctaagag gaaaatattg
     4861 ataccataaa attaaacttg ctaccgctaa aattgagagc caaaaactat cagaaagcag
     4921 gctcaatggg tcaattaaaa tcagtaacgc cactattctc ccccaatatt gccaaggggt
     4981 atagtgtcgc cgcagaactt ggcaaagcaa gacaaacgca atcgccagca acgctctggc
     5041 ggtcggcacg gcaaaaccgg ctaaatagct gtaaccaaat gcggtgatga agctaactat
     5101 tcttgcaaaa taaagtgaaa atccgaccgc ttgtaatgcg gtaagctcgc ttttcaagca
     5161 aagccattgc acgcctttgg caaagtaaac accaaacaac attgccagcc cgatatgcaa
     5221 gcctgaaatg gcaattagat gggcagtagc ggtttgttgg aattgccccc agtgttctgt
     5281 tttcagccac gccctttcac caaaagccag agccaataaa aagccttgcg taggcaaagc
     5341 gtccgtttca gctttcactt tattcagcca atttgttcgc aagctataat cagttttggc
     5401 taatgtttcc accttgcgaa ccgttgctgt catattgata tgattggcaa aataccaacg
     5461 ctgtcggtca aaattaccga tattgctacg agctgaaata ggacgaagag ccagttcggc
     5521 ttgatagaat tgattaagta acaacggctg tttcgattgc cagttcaagt agatcttctc
     5581 gcctgtattt agtgtcgcaa tcgctgtttg ataatcggtt tgttttaaga ttttactgat
     5641 ttcaaattga acaggcgttt tactagcagt cgttttttcc gcattttttg caacttgaat
     5701 gatctgaata tagccaatac aaaccaataa gccgagtaca gccgttaatt ttgagcgtag
     5761 ataaacactc aatatcagca ctaaggttcc tattactata cccacaaata gaaacgcttt
     5821 aggcaaccat aaaataggca gatgaaatag aatcagggca atacaaaaac ggtctaaagt
     5881 cataattcgg cttacttttt aaagctgagt atgccgtttg tgcttaatat tttcagaata
     5941 aattttcaga ttttcgacac agttcgcaaa aatacttgaa aaaataaagg cagacttttt
     6001 agcctgcctt ataaagaaga gatattatgc tgatttttga tcttcagcgt aacttactgc
     6061 tgcagtgaat aatacgtctg ttgatgaatt taatgcggtt tcaaccgagt cttgaatcac
     6121 accaatcaca aaacccaccc caattacttg agcagcaata tcgtttggaa tattaaataa
     6181 actacaggct aatggaatca ataataatga accgcctgcc acacctgatg cgccacaagc
     6241 acagattgat gcaactacgc ttaatagtag tgctgttgcg aaatcaggtt gaataccttg
     6301 tgtataagct gccgctaggg ttaatacggt tacagtaatc gctgcacctg ccatattaat
     6361 acttgcacct aatggaatag ctacagaagc gatctcatca cgaacgccca aacgcttggc
     6421 taaattcata ttcaccggaa tgttggctgc tgaactgcgg gtaaagaatg ccgttacacc
     6481 actctctcgt aaacaggtaa atgtaagtgg gtatgggttg cggcgaattt tccaatacac
     6541 taataatgga tttagtacta atgccacaaa taacattgcg ccaactaaga ctactaataa
     6601 acgtgcgtaa tcttcaaacg catcaatacc attgatggca acagtttcag ctactaaacc
     6661 aaatacccca ataggcgcaa atgcaatcac aactttcaca acaaacgaaa ctgcgttagc
     6721 taaatcattt aagaatgctt ttgaacttgc cgaaccatgg cggaatgcaa tccctaaacc
     6781 gatagaccac gcaagaatac cgataaagtt tgcattactt aatgcgacta atggattatc
     6841 aactaagtta aaaataacgg tttttagaat ttcaccaatg ccttgtggcg gggttaaacc
     6901 gtctgggctt gcagtaagct ctaatgtagt tgggaacata aagcttaaaa caacagccgt
     6961 taatgccgca aaaaatgtac ctaatacata aagcaccaga atcggcttta atttgctatc
     7021 tgaaccaact tctttatttg caatcgcagc caaaactaat acaaatacta aaataggcgc
     7081 aatggcacgt agtgatttca caaaaaattg acctaataca ccaacgctgg tagcccattc
     7141 tttattaata aatgccagca atacccctag cactaaacca atggcgatac gtaatactaa
     7201 attaccgcca agaatttttg atgataatga t
//
LOCUS       DANLRK010000133         7116 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.133, whole genome shotgun sequence.
ACCESSION   DANLRK010000133 DANLRK010000000
VERSION     DANLRK010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7116
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.133"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            <1..573
                     /locus_tag="PXA33_001978"
     CDS             <1..573
                     /locus_tag="PXA33_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HDL5121284.1"
                     /translation="LNELKTSIVWQGRTYDPYPIEATGFERNSSGPSNRPTLTLSNLF
                     GLVTGIANQFDECIGAIVRRHQVYAQYLDAVNFAGGNAKADPNQEIISHFVIEQLSSL
                     TRETATFTLALPIETDNAKIPSRIIMADTCTWIYRSAECGYTGKQYFDEKDKATSDPK
                     ADKCSHCLNGCELRGNQRNFGGFVSVNKLG"
     gene            577..1308
                     /locus_tag="PXA33_001979"
     CDS             577..1308
                     /locus_tag="PXA33_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDL5121285.1"
                     /translation="MELEQQIIDYALQHEPHEMCGFVVFDGNKNRFIPCENQAEDKAN
                     YFEISDLDYIKAEEKGELVAVVHSHPEPNGKPILSVLDRKMQVQTGLDWWLVHNRQIH
                     KFRNVPHLIGREFKHGTMDCYTLYRDAYMLAGYEMDEFERQDDWWHTGQNLYLDNIQG
                     QGFERVETPQIGDVILMQVGADVPNHAAIYIGDQMVVHHSPNRLSKRDLYDGYWLRHT
                     HSIWRHKLADKLDFDGILNDIAVNN"
     gene            1577..2206
                     /locus_tag="PXA33_001980"
     CDS             1577..2206
                     /locus_tag="PXA33_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HDL5121286.1"
                     /translation="MITVKFYGHLKTFGTDFKILVKDIAEAIRALCSQLKGLREALRD
                     GVYKVRIGKQYLDPSALEKGLFYCLKKGQTIHFTPVVKGAKSGGIFQAVLGVALIGAA
                     FALGPIGLGLVGSATAMTVGAMGASMLLGGVSQMLTKMPKAPTMGNETEKESSTAFSN
                     LNNLVAQGKPVPLAYGLIRTGSLVISQGVETITIKENQPASNKKTGFRK"
     gene            2216..>7116
                     /locus_tag="PXA33_001981"
     CDS             2216..>7116
                     /locus_tag="PXA33_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121287.1"
                     /translation="MRIYGAKGGGGSSHTPIEAPETGRSKQIVNIVELLCEGEIEGLV
                     DGFKSIYLDGTQIQNDDGTYNFNNVSGQLNVGTQDQDVLDGYDSSQNEVNVGVEIKKK
                     NGAIVRTVTDERINRLRLTLGVRSLFHQNNQGDTNTTNVDLKITIGTRQYSHSFNGKY
                     SSQYLESVVFDNLPPVPFNISVERLTEDSNSQRLQNGTIWSSYTEIIDTEFTYPNSAV
                     AGISFDSEYFNNIPTRNYLIKAKKVKVPSNYDPVKRTYTGFWDGTFKVAWTNNPAWEI
                     YDLAPILSKMLGVEISFDKWALYDVSRYCDQLVPDGMGGQEPRFTCDVWLTEVKTAYD
                     LLNDFCSVFRAIPIWTGTEVSVIIDRPRDPVWTYTNANVVSGFERSYSARKSRHNAVQ
                     VTYSDKTNGYESAIEYVSDDEEIKKHGLNLSQITAFGCTSRGQAHRTGKWILETEKRE
                     KETITFTVGREGLMHLPGDIIRVADSHYAGTEIGGRVLAINGRKVTLDREISIDNASY
                     FTYINGEATHSTIKIQSVNGKEITLDSTPTGLETYGVWSLSTKQISSGLYRSISIVEN
                     ADGTNTITALQHEPQKEAIVDNSAHFVETARTLYKAPQINAVEVSTGYDGKLYITGDI
                     SSGDGKLTYDIKITKDGNLYQYKKGLSDPNIELSDLPNGDYTVIIYGKNAKGQIVTEK
                     TQTFTIDRPPAPTGVVVTGGLGQITLEWDWVNEVTQTEIFAAETDNFALAKKIAKVTA
                     RTYAHTLKGNKVVRYYWLRHTRGINVGPFYQQQGVRGQTAVDLDARLTELNTQLSRNI
                     VNEVFDVAAPARGLELVKTVANLTDKGTKLASSQVYNQADGKLYTWNGTAYSATVAAE
                     DVTGKLSKSKIDTSLISQLTGADNTANLARRLAETAQSNINQEITNRQDAVTAEANNR
                     TKAIQAESANLTKKIQAEATARGAAVTQLQNVDAQQAQLISAVTAKADNALSGLEEEK
                     TARANADKAESQAREALTARMGAAESNIVTIQRTVANNAQSISEVSQNLNAKIDNINI
                     GGRNLLRDSEFNAYNKWGSPQIEFAENANRRTIKVTSTGTNGPVGIVSSNRHSTSYFQ
                     QGETYTLSLFARGSKALDYLYLMRQDGNNVRLPVINVASETEFNHYKLTFKAPFTTQQ
                     GYVLIGFRQTSTEQFVEFHSVKLEKGNVATDWTPAPEDVESAVSAVSADLTSYKQTQA
                     ATDSAQAQQLNQLSVNLTKAETNFNAKITEEKKARVDADKVNADKLTDITSRVANAES
                     TITNFQSTKANKSEVASIAQQNLQSIWRTDAQSAVDALKIGGANLLVDSEYLTTARWG
                     GSSRVASSQYGDRRLTQVFVTQAGTGHFGVTQGTQKATTRIRQGETYTLSLNAQGTAG
                     FTRTGLNYVYLIREDGGNFRLPTLPLTASLSQRPKVTFTAPWTSNQVRLLIGANGIFE
                     ATDWFAFHSVKLEMGNVATGWTPTAKDIDDKVSAVQSNLTAYQAAQAKADQAKATQIS
                     GLTTRMGAAESNLTRTERAVTELNQTTVTTLRDLTARTKTTEGSLSRLETAKASKTEV
                     ASIAQSSLQSIWKADAKSAVDSLSIGARNLLIDSTYNQHINYNTKMSSITRTVYRGNM
                     LIRLGMLGGAGVAGIVQAPAAQV"
BASE COUNT         2213 a         1523 c         1618 g         1762 t
ORIGIN      
        1 ctaaatgaac tcaaaacaag tattgtctgg caaggtcgaa cttacgaccc ttaccccatc
       61 gaggcaacag gatttgagcg taacagcagc ggcccaagca atcgccctac tcttacgcta
      121 tcaaatctgt ttggcttagt tacgggtatt gctaatcagt ttgacgaatg tatcggtgcg
      181 attgttcgcc gtcatcaggt ttacgcccaa tatttagatg cagtaaactt tgctggaggt
      241 aatgccaaag ccgatcctaa tcaggaaatc atcagccatt ttgtgattga gcaactttca
      301 agcctcacac gagagactgc tacttttacg cttgcgttgc cgattgaaac cgataatgcc
      361 aaaatcccca gcagaattat tatggcggac acctgcactt ggatttaccg ctcggcggaa
      421 tgtggctaca ccggcaagca gtattttgat gaaaaagaca aggcaacatc agacccaaaa
      481 gccgataaat gcagccattg cctgaacggt tgtgaattgc gaggcaatca gcgtaatttt
      541 ggtggatttg tatcggtgaa taaattgggg taatgaatgg aattggaaca acaaataatc
      601 gactacgccc tccaacacga accgcacgaa atgtgcggct ttgttgtttt tgacggcaat
      661 aaaaatcgat ttatcccttg cgaaaaccaa gcggaagaca aagccaatta ctttgaaata
      721 tccgatcttg attacatcaa agcagaagaa aaaggcgaac tggtagcggt ggtacattca
      781 catcccgagc caaacggcaa gcctatatta tctgtgcttg atcgcaaaat gcaggtacag
      841 accggtttag attggtggct agtgcataat cggcaaatcc ataaattccg caatgtacca
      901 catttaatcg ggcgtgagtt taaacacggc acaatggatt gttacacgct ctaccgtgat
      961 gcctatatgc ttgccggtta cgagatggac gagttcgagc gacaagatga ctggtggcat
     1021 accgggcaaa atctttattt ggataatatc caagggcaag gttttgagcg agtggaaacc
     1081 ccacaaatcg gcgatgtgat tttaatgcaa gtaggtgctg atgtgccgaa ccacgctgcc
     1141 atttacattg gcgatcagat ggtagtccat catagcccca accgtttatc taaacgtgat
     1201 ttatacgacg gctactggtt acgtcatacc cacagcattt ggcggcataa actggcggat
     1261 aagttagatt ttgacggcat attgaatgat attgctgtaa ataactaaca aaaaatgacc
     1321 gcttgtaaca aaaacaaacc ccgaagcgtt tgcagcactt cggggttttt catatccact
     1381 tacctaacta agaggacata aattgaatga atgataaacg atttacattc aaatttctag
     1441 gagttcttat ggaagcaata aatattaccc caaaagaaat cagaaaaaca atgtggacag
     1501 ccgcaataat tctctttttg tttgcgttca tctggaaagc ccctgaattt atcactgcta
     1561 ttcgttggtg gtaactatga ttacagttaa attttacgga caccttaaaa cattcggcac
     1621 agattttaaa attttagtta aagacatcgc tgaggcaatt cgtgctctgt gttcacagct
     1681 taaaggattg cgtgaagcgt tgcgagacgg tgtttataaa gtccgtatcg gtaagcagta
     1741 tttagaccca tcagcccttg aaaaagggct tttttattgc ctgaagaaag ggcaaaccat
     1801 tcattttacc cctgttgtca aaggagcgaa aagcggtggt atttttcaag cggtgttagg
     1861 tgtggcgtta attggggctg catttgcgtt aggtcctatc ggcttaggat tggttggctc
     1921 tgctacagca atgacagttg gagcaatggg ggcatcaatg ttactaggag gagtttccca
     1981 aatgctcacc aaaatgccaa aagcccccac aatgggtaat gaaacagaaa aagaaagctc
     2041 aacggctttt tcaaatttaa ataacttggt ggctcaaggc aaacctgtgc cgttggctta
     2101 tggtttgatt cgtaccggct cgctggtgat atcgcaggga gttgagacga tcaccattaa
     2161 agaaaaccaa ccggcaagca acaagaaaac cggctttaga aaataaggga taattatgcg
     2221 tatttacgga gcaaaaggtg gtggcggcag tagccatacc ccgattgaag caccggaaac
     2281 cggtcgctca aaacagattg tgaatattgt ggaattgctg tgcgagggcg aaattgaagg
     2341 cttggttgat ggctttaaat cgatttacct agacggcacg cagatccaaa acgatgacgg
     2401 cacctataat tttaataatg tgagcggtca gctaaatgtc ggtacgcaag atcaagatgt
     2461 gttggacggt tacgatagct cacagaatga agtgaatgtg ggtgttgaga ttaagaaaaa
     2521 gaatggggct attgtccgca ccgtaactga tgaacgaatt aaccgcttgc gtttaacgtt
     2581 gggagtaagg tcattattcc accaaaataa tcaaggcgat accaatacca ccaatgttga
     2641 tttaaaaatc acgattggca cacggcaata ttcgcatagc tttaacggca aatacagctc
     2701 gcaatacctt gaatctgtgg tgtttgataa cctgccgcct gtgccattta atatctcagt
     2761 tgagcgtcta accgaggata gcaattcgca acgcttacaa aacggtacaa tttggagtag
     2821 ttacaccgaa attatcgata ccgagtttac ctaccctaat tcagcggttg ccggtatcag
     2881 ttttgattcg gagtatttta ataatatccc gacccgaaac tacctaatta aagcgaaaaa
     2941 agtcaaagtg ccaagcaatt atgatccagt taaacgcact tacaccggct tttgggacgg
     3001 cacatttaaa gttgcgtgga cgaacaaccc tgcttgggaa atttacgatt tagctccgat
     3061 tttaagcaaa atgcttgggg ttgaaatcag ctttgataaa tgggcgttgt atgacgtctc
     3121 tcgctattgc gatcagcttg tgccggacgg tatgggcgga caagagccac gttttacctg
     3181 tgatgtatgg ctcacggaag ttaaaactgc ctatgattta ttaaatgatt tctgctcggt
     3241 attccgtgcg attccgattt ggaccggcac ggaagtctcc gtgattatcg accgtcctcg
     3301 tgatccggtt tggacttata ccaacgctaa tgtagtcagc ggttttgaac gctcatattc
     3361 tgcccgcaaa tctcgccata atgcggtaca agtgacctat tcggataaaa cgaatggcta
     3421 tgaaagtgcg attgaatatg tctctgacga tgaagaaata aaaaagcacg gcttaaattt
     3481 aagtcaaatt accgcctttg gttgcacctc tcggggacaa gcacaccgta ccggtaaatg
     3541 gattttagaa accgaaaaac gagaaaagga aaccattacc tttaccgttg gacgtgaggg
     3601 cttaatgcac ttaccgggcg atattatccg tgtagccgat agccattatg ccggcacgga
     3661 aattggcgga cgagtgttgg cgattaatgg gcgaaaagtt acccttgacc gtgaaattag
     3721 cattgataac gccagttatt ttacttacat caacggtgag gcgacacact caaccatcaa
     3781 aatccaatct gtaaacggca aagaaattac gctggatagt acaccaaccg gcttagaaac
     3841 ctacggtgtt tggtcgttat cgactaaaca aatcagctct ggattatatc gctcaatctc
     3901 gattgtcgaa aatgcggacg gcacaaacac tatcacagcg ttacaacacg aaccgcagaa
     3961 agaggcaatt gttgataact ccgctcactt tgtggaaacg gctagaacac tctacaaagc
     4021 tccacaaatt aacgcagtgg aagtctcgac cggttatgac ggtaagctct acattaccgg
     4081 tgatattagc tccggtgatg gcaagctaac ctatgatatt aaaattacca aagatggcaa
     4141 tttatatcaa tacaaaaaag gcttaagcga tccgaacatt gaactaagtg atttacctaa
     4201 cggcgattat accgtcatta tttacggcaa aaacgccaaa gggcaaatcg tcaccgaaaa
     4261 aacacaaacc tttactattg atcgacctcc agctccgacc ggtgtagtag taacaggtgg
     4321 attagggcaa attaccctcg aatgggattg ggtaaatgag gttacgcaaa ccgagatttt
     4381 cgcggccgaa accgataatt tcgcccttgc aaaaaaaatc gcaaaagtga ccgctagaac
     4441 ctacgctcac acactaaaag gtaacaaggt tgtacgctat tattggctac gtcataccag
     4501 aggcattaat gtcggaccgt tttatcaaca acagggggta agaggacaaa cagcggttga
     4561 tttggatgct cgattaaccg aattaaacac tcagttatca cgcaatattg ttaatgaggt
     4621 ttttgatgtt gctgcaccgg caagaggttt agaattagtg aaaacagtcg ctaatttgac
     4681 cgacaaaggt actaaactgg cgtcatctca agtttacaat caggctgacg gcaagctcta
     4741 tacttggaac ggtacagcat actcagctac agtggctgcg gaagatgtta ccggcaaatt
     4801 atcaaaatct aaaattgata cgtcccttat ttcgcaatta acaggtgcag ataacaccgc
     4861 aaatttggca agaagattag cggaaacagc tcaaagcaac attaaccaag agattactaa
     4921 ccgacaagat gccgttactg ccgaagcaaa taaccgtact aaagccattc aagccgaaag
     4981 tgcgaattta acgaaaaaaa ttcaagctga agcaaccgct cgtggggctg ctgttacaca
     5041 gttacaaaat gttgatgctc aacaagcaca gttgatttct gctgtcacag caaaagctga
     5101 taatgctctt tcaggcttgg aagaagaaaa aacagcacga gcaaacgccg ataaagcgga
     5161 gtctcaagca cgtgaagcat taacagcaag aatgggagca gctgaaagca atatcgtaac
     5221 tattcaacgt acggtagcaa ataatgccca aagcattagt gaagtcagtc agaatttaaa
     5281 tgctaagatt gataatatta atatcggcgg tcggaattta ttaagagaca gcgagttcaa
     5341 tgcgtacaat aaatggggta gcccacaaat tgaatttgct gaaaatgcaa atcgccgaac
     5401 tatcaaagta acctcaacag gtactaacgg tcctgttggt attgtttcat ctaatcgcca
     5461 ttccacatct tatttccaac aaggagaaac ttatacactc tcattatttg ctcgaggaag
     5521 taaggcacta gactatctct atctaatgcg tcaagatggg aataacgtta gattaccagt
     5581 gattaacgtt gcctctgaaa ctgagtttaa tcattacaaa ctcacgttca aggctccttt
     5641 taccacgcaa caaggttatg tcttaatcgg ttttcggcaa accagcacag agcagtttgt
     5701 tgagtttcac agcgtcaaac ttgaaaaagg caacgtagcc acagactgga caccagcacc
     5761 ggaggatgtg gagagtgcgg ttagtgctgt ttcggctgat ttaacgagtt ataaacaaac
     5821 tcaagcggct accgattcag cacaagcaca gcaacttaat cagttatctg tgaacttgac
     5881 caaagcagag actaatttca acgccaaaat cacggaagaa aaaaaagctc gtgttgatgc
     5941 agataaagtg aatgcagata aactcacaga cataacaagc cgtgttgcaa atgcggaatc
     6001 aactattacc aatttccaat caaccaaagc gaataaaagt gaggtggcaa gtattgccca
     6061 gcagaactta cagagcattt ggcgcaccga tgcgcaaagt gcggtggatg ccttgaaaat
     6121 cggtggtgca aatctactgg ttgatagtga gtatttgacg acagcccgtt ggggcggtag
     6181 ctcacgagtg gcaagctcgc aatatggtga tcgtcgcttg actcaagtgt ttgtgaccca
     6241 agcaggcaca gggcattttg gtgtgactca aggcacacaa aaagccacta cgcgtattcg
     6301 tcagggtgaa acctatacct tgtcactaaa tgcgcagggg acggcaggtt ttacccgcac
     6361 agggttaaac tatgtgtacc ttatccgcga agatggtggc aattttagat tgccaacttt
     6421 gccgcttact gcatcattgt cccaacgccc taaagtgaca tttactgcgc cgtggacgag
     6481 caatcaagtt cggttactga ttggcgcaaa tggcatattt gaggcgacag actggttcgc
     6541 atttcacagc gttaagctcg aaatgggtaa tgttgccaca ggctggacac caaccgccaa
     6601 ggatattgac gacaaagtat cagcggttca gtccaacctc accgcttatc aggcggcaca
     6661 agctaaagca gatcaagcca aagctacgca gatttcgggg ctcacaaccc gaatgggggc
     6721 agcggaaagt aacctcactc gcaccgaacg ggcagtgacg gaacttaatc agacaacggt
     6781 gacgacactg cgtgatctaa ccgcgcgcac caaaacaacg gaaggttcgc tctctcgttt
     6841 ggaaaccgcc aaagcaagta aaactgaggt ggcaagtatt gctcaaagct ccttacagtc
     6901 aatttggaaa gctgatgcaa aatctgccgt tgattcactt tctatcggtg caaggaactt
     6961 actgatagac agtacttaca accaacacat caactacaat acaaaaatgt catcaatcac
     7021 acgcacagtc tatcgcggca atatgttgat acgactcgga atgcttggtg gcgcaggtgt
     7081 ggccggtatt gtacaagcac cagccgctca agtaag
//
LOCUS       DANLRK010000134         7025 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.134, whole genome shotgun sequence.
ACCESSION   DANLRK010000134 DANLRK010000000
VERSION     DANLRK010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7025
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.134"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(<1..617)
                     /locus_tag="PXA33_001982"
     CDS             complement(<1..617)
                     /locus_tag="PXA33_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL5121288.1"
                     /translation="MAEILFRSCLCCNAKRIFYLLTFYKGFETSQIATAFSLLVFTSQ
                     AFSLFAGSWGDSFGRYPIMMLGCLLDALAYILLLTTKNYPLLLLATFFFGLGSTLFST
                     NARAFLLANIQDTGDSYQLKTKAQGKFLKVSSLSAMVAPLLTIPFIFYRKAEWLIWIS
                     CCIEIIMLFVMFKTAPKTHECANGQCERFKFGALKGILNEQFIFV"
     gene            700..1161
                     /locus_tag="PXA33_001983"
     CDS             700..1161
                     /locus_tag="PXA33_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HDL5121289.1"
                     /translation="MRKIKLLHVILSLILGLSAAAIGSYPAIKEIGEERAKKHQDILD
                     LNRELYSAMVNRDFSALQTLLSDDFELYSSTGEVIDKQEWIKLIQKRVLEFDKVDLLS
                     TDFQGNQSSGLLRVNGEFFGIERDNVVVEMNIRTIVRNTERQIKCIVIKEV"
     gene            1174..2067
                     /gene="murQ"
                     /locus_tag="PXA33_001984"
     CDS             1174..2067
                     /gene="murQ"
                     /locus_tag="PXA33_001984"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HDL5121290.1"
                     /translation="MNALGQMITEQRNPNSMNIDRLSALDIVQVINQEDKQVAIAVEQ
                     CLPQIAQAVEKIVQAFEKGGRLVYVGAGTSGRLGVLDASECPPTYGVKPEMVVGLIAG
                     GERALRFPIEGAEDNREQGKVDLQAVNFSEKDVLVGIAASGRTPYVLGAMEYAKQLGA
                     TTVSIASNPNSAMAQIADIAIETVVGPEVLTGSSRMKSGTAQKLVLNMLTTASMILIG
                     KCYQNLMVDVQATNEKLKARAIRIVMQATECEREVAEDTLKLAENNAKLAIMMILGNL
                     DKQKAEDLLSQNQGRLQKALK"
     gene            complement(2151..3095)
                     /gene="prmB"
                     /locus_tag="PXA33_001985"
     CDS             complement(2151..3095)
                     /gene="prmB"
                     /locus_tag="PXA33_001985"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDL5121291.1"
                     /translation="MFKYNTELLDEISALPIVNDLVTIQDMMRWGYSYFNASDLYYGH
                     GYDNAWDEAQQLVLAGLYLPFDVPETLYSSRLTRVEKERIINLICERLGSRKPVAYLT
                     NSAWFCGGEYYVDERVIVPRSPIGELIKKGFKGIIKNEPHRILDMCTGSGCIAIACAE
                     RFPEAEVDAVDLSLDALDVAQINIERHQVSHRVFPICSDLFNNLPQDKYDLIVTNPPY
                     VDEEDLGDMPEEFHHEPALALGSGKDGLDLTKRILAQAADYLNDDGVLVCEVGNSMVH
                     LIEQFPSVPFNWLEFEHGGLGVFSLTKEELVKHRHLFA"
     gene            3188..3733
                     /gene="smrB"
                     /locus_tag="PXA33_001986"
     CDS             3188..3733
                     /gene="smrB"
                     /locus_tag="PXA33_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HDL5121292.1"
                     /translation="MNDTLLDDEFALFREAAKGTKKLTQNTFVPKAEPRKKLQELREL
                     KEKADTEFYFSDEYEPLLKEENEKVKYLREDVDPYILKQLRRGDFQPELFLDLHGLTR
                     EKAKKELAALILACEREKVYCASVMTGFGTRTLKDQIPRWLVQHPKVMALHQAPKEWG
                     GDASILILVEQPENPEKMFGR"
     gene            3797..4282
                     /locus_tag="PXA33_001987"
     CDS             3797..4282
                     /locus_tag="PXA33_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HDL5121293.1"
                     /translation="MSEQTFHQLTALLDSHRARYRVVGHPSAGKSEEVAKIRGTEIGQ
                     GAKALVCKVKGNGVKQAVLAILPADYQADLTKIAEHLGGLRASLASPAEVAELTDCVF
                     GAIPPFSFHPDLLLVAEPSLFERYAELAFNAGTLERSIILNTEDYQKIVNPVLVQFAK
                     S"
     gene            complement(4284..5033)
                     /locus_tag="PXA33_001988"
     CDS             complement(4284..5033)
                     /locus_tag="PXA33_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDL5121294.1"
                     /translation="MEKRFEINEQGLSIRCKLFYEKELQQIENVVIVLHGFGSSKDLK
                     SNTKFGERLSSKYKGYAALAFDFPCHGDDARKKLSVEESITYLQLVIDYAKTSLNAKN
                     LYAYGTSFGGYLTLKYLAEKGNPFRKIALRAPAVQIYHSLMNRLSEEERQKLAKGREI
                     LWGFDRQMKISQAFFDELTDITQNDYLDYAEQILILHGTEDEMVALSTSQQFADNNVI
                     ELLPIEGADHAFSNPKLMDIAIGKIVEFFAP"
     gene            5205..6068
                     /locus_tag="PXA33_001989"
     CDS             5205..6068
                     /locus_tag="PXA33_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA/RhaT family transporter"
                     /protein_id="HDL5121295.1"
                     /translation="MLQLILAILCSVTVGVLIKFARQKGIRIDQSIAVNYIVTTTMTF
                     LLLKPDYQGQSITDIIANNPTSYIFVILSITLPTIFLVQAKALEFAGIIRTDAAQRLS
                     LFLPILAAFTIFGEQVTQNKLIALLLAFVALGCLLWKGNQGMEKGGKIAIISLALVWV
                     GYGVNDILFKQIAKSGSAFPVTLSIAFIGAGCFMFIYLLLKRTQWHVPSVLTGLLLGV
                     LNFGNILFYIQAHQAMKDDPTLVFTGMNLGVICLGTFVGAFFFKEKIHKVNYLGVAIA
                     IVAIICLFYWR"
     gene            6192..6785
                     /locus_tag="PXA33_001990"
     CDS             6192..6785
                     /locus_tag="PXA33_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121296.1"
                     /translation="MKLKALSTLIASSMLYSQSFAVPPEKTAEEPQPFFNKTESGYDL
                     VFNDQNYRLIETELNGEKIQFRAFERIVYVNNPIEPDYQTINLYVPEAYYNNGEINGY
                     QADTAPIFLPNSVGGYMPAKAASYDKKRRDGSPGTILVALSKGYVVASVGARGRTLQK
                     EGKYTGKAPSAIIDSKSAAHKACRFESDHWHHSLCKI"
BASE COUNT         2160 a         1327 c         1541 g         1997 t
ORIGIN      
        1 tgtacaaaaa taaattgttc atttagtatt ccttttaatg caccaaattt aaagcgttcg
       61 cattgcccat ttgcacattc atgtgttttg ggtgcggttt taaacataac gaatagcata
      121 ataatttcaa tgcagcagct aatccaaata agccattctg ctttacggta gaaaataaat
      181 ggaatagtaa gtaatggtgc taccatcgct gacaggcttg ataccttcag aaatttacct
      241 tgagccttag ttttgagttg gtaactatct ccggtatctt gaatattggc taataagaat
      301 gctctcgcat tggtactaaa taaggtgcta ccgagcccga agaaaaaggt agcaagtaat
      361 agcagagggt aattttttgt tgtcagtaat aaaatgtaag ctagggcatc gagcaaacag
      421 ccaagcatca tgatagggta acgtccaaaa ctatcacccc aagaacctgc aaaaagggaa
      481 aatgcttggc tggtaaagac caacaaagaa aatgcagttg caatttggct ggtttcaaag
      541 cctttgtaga atgtaagtag ataaaaaata cgctttgcat tgcagcataa gcaagaccgg
      601 aaaagaattt ccgccataaa atgatatttt ggatattcat agataatatt taatgagaaa
      661 aatgatttct aatataacta aatttaataa ggaagcacaa tgagaaaaat caaattattg
      721 catgttattt tatctttaat attagggctt tctgcggcag cgattggaag ttatccggca
      781 ataaaagaga ttggcgagga gcgggcaaaa aaacatcaag atatattaga tttaaataga
      841 gaactttata gtgcaatggt gaaccgtgat ttttcggctt tgcaaacatt actaagcgat
      901 gattttgagc tttattcatc aacaggtgaa gtgattgata agcaagaatg gatcaaatta
      961 atccaaaaga gagtattgga atttgataag gttgatttgc tatctaccga ttttcagggg
     1021 aatcaatcat ctggattatt aagagtaaat ggtgagtttt ttgggataga gcgggataat
     1081 gtggtggtag aaatgaatat tcgaaccatt gtgagaaata cagaacgcca aattaaatgt
     1141 attgtgatta aagaggttta agtgtcgcaa ctattgaatg ccttagggca gatgattacc
     1201 gaacaacgta atccgaattc gatgaatatt gaccgccttt cagcgttaga tatagtccaa
     1261 gtcatcaacc aagaagacaa acaagtagcg attgcggtgg agcagtgcct gcctcaaatt
     1321 gcacaagcgg tggaaaaaat tgtgcaagcc tttgagaaag gtggacgatt ggtttatgtc
     1381 ggtgccggca cgagcggaag attaggggtg cttgatgctt cggaatgtcc gcccacttat
     1441 ggcgtaaaac ctgaaatggt ggttggatta attgccggtg gtgagcgggc attacgtttc
     1501 ccgattgaag gggcggaaga taatcgtgag caaggcaaag tagatttaca agcggtcaat
     1561 tttagcgaaa aagatgtatt ggtcgggatt gcagccagtg ggcgaacgcc ttatgtgtta
     1621 ggggcgatgg aatatgctaa gcaacttggt gcgaccacgg tgtcgattgc cagtaaccca
     1681 aattcggcaa tggcacaaat tgcggatatt gcgattgaaa ccgttgtcgg gccggaagtg
     1741 ctaaccggtt ccagccgtat gaaatccggc acagctcaaa aattggtatt gaatatgctc
     1801 actactgcgt cgatgatttt aatcggtaaa tgctaccaaa atttaatggt agatgtgcaa
     1861 gcaactaatg aaaaactcaa agctagagca attcggattg tgatgcaagc gacagaatgt
     1921 gaacgagaag tggctgaaga tacactaaaa ttagccgaaa ataacgctaa attggcaatt
     1981 atgatgattt taggtaactt agataagcag aaagccgaag atttattaag ccaaaatcaa
     2041 gggcgattac aaaaagcgtt aaaatagaag tatgtctgct ctccccttgt gggagagaga
     2101 cagatcaatc attcattcag aatgattgat cagagagagg ggaggcagat ttaagcaaat
     2161 aaatggcgat gtttcactag ttcttctttg gttaaactga acacccctaa gccaccgtgt
     2221 tcaaattcca gccagttaaa cggcacactg gggaattgtt cgattaaatg caccatactg
     2281 ttgccgactt cgcagactag cacgccatca tcatttaagt aatcagcggc ttgagcaaga
     2341 atccgcttgg ttaaatctaa cccatctttg cccgagccaa gagctaatgc cggctcgtga
     2401 tggaactctt cgggcatatc gcctaagtct tcttcatcaa cataaggcgg attagtcaca
     2461 attaagtcgt atttatcttg cggtaaattg ttaaacagat cggaacagat cgggaaaact
     2521 cggtgagaca cctgatgacg ctcaatatta atttgagcaa cgtctaaggc atcaagggaa
     2581 agatcaaccg catcgacttc tgcttccgga aaacgctcgg cacaggcaat cgcaatacaa
     2641 ccgctgccgg tacacatatc taaaattcga tgtggctcgt ttttgataat gcctttaaac
     2701 ccttttttga tcagctcacc aatcggtgaa cgtggcacga tcacacgctc atcgacataa
     2761 tattcaccgc cacaaaacca agccgagttg gtaaggtatg ctacaggttt gcgagagcct
     2821 aaacgctcac agattaaatt gatgatacgt tctttctcaa ctcgagttaa gcgagaggaa
     2881 taaagtgttt ccggtacatc aaacggcaaa taaagccctg ccaacactaa ttgttgtgcc
     2941 tcgtcccacg cattatcgta gccgtgcccg taataaagat cagaggcatt aaaatagcta
     3001 tacccccaac gcatcatatc ttgaatagtg actaaatcat tcacaatggg cagggcggaa
     3061 atttcatcaa gaagttcggt attatattta aacattggcg tgcctttttt cgtattgtta
     3121 aaatggctgt gttataccac attttgcttt gtgttagaat gtaaaaatga ataagaagga
     3181 aaagataatg aacgatacgt tattagacga tgaatttgca ttattcagag aggcagcaaa
     3241 aggcacgaaa aagctgacac aaaatacctt tgtgccgaaa gcggaacctc gcaagaaatt
     3301 gcaagaattg cgagaattaa aagaaaaagc tgatacagaa ttttatttct cggatgaata
     3361 tgagcctttg ctgaaagaag aaaatgaaaa agtgaaatat ctgcgagaag atgttgatcc
     3421 ttatattctc aaacagttac ggcgaggcga tttccaaccc gagctgtttt tagatctgca
     3481 tggtttaact cgagaaaaag cgaagaaaga gttggcagcg ttgattttag cctgtgagcg
     3541 ggaaaaggta tattgtgcca gtgtgatgac cggctttggc actcgcacgc tcaaagatca
     3601 aattccacgc tggttggttc agcacccaaa agtaatggcg ttgcaccagg ccccgaaaga
     3661 gtggggcgga gatgcctcta ttctaatttt ggttgagcag cctgaaaatc ctgaaaaaat
     3721 gtttgggcgt taaaattcgg ctgaattgcc aattttttat taaaaacgac cgcttgtgta
     3781 tcattaagga gaaatgatgt cagaacaaac tttccaccaa cttacagcat tattagattc
     3841 gcatcgagct cgctatcgag tggtaggaca tccttctgcc gggaaatcgg aagaagtggc
     3901 aaaaattcga ggaacagaaa tcgggcaagg ggcgaaagcc ttagtttgca aggtaaaagg
     3961 caatggggta aagcaagctg tgcttgctat attacctgct gattaccaag cggatttaac
     4021 aaaaattgca gagcatcttg gtgggttaag agcctcactt gccagcccgg ctgaagtggc
     4081 agaattgacc gattgtgtat ttggggcaat tccgccgttt agctttcacc ccgatttatt
     4141 attggtggct gagccaagtc tgtttgagcg ttatgctgaa ttagccttta atgccggtac
     4201 gttggaacgt tcgattattc tgaataccga ggattaccaa aagatagtta atcctgtgtt
     4261 ggttcaattc gccaaaagct aggttatggg gcgaaaaact ccacgatttt gccaatagcg
     4321 atatccatta atttagggtt agaaaaagcg tgatctgccc cttcaatcgg gagcagttca
     4381 atcacgttat tatcggcaaa ctgctgtgaa gtgcttaacg ccaccatttc atcttccgtg
     4441 ccgtgcagaa ttaaaatttg ctcggcataa tccaaataat cgttttgggt aatatcggtt
     4501 agctcatcaa aaaaggcttg gctgattttc atctggcgat caaaccccca caaaatttct
     4561 cgccctttgg caagtttttg ccgttcctct tctgacaagc ggttcattaa cgaatggtag
     4621 atttgtactg ccggtgcccg caatgcaatt ttccgaaacg gattgccttt ttccgccaga
     4681 tatttcaagg ttaaatagcc gccaaaacta gtgccgtagg catacagatt tttagcattc
     4741 aggctggttt tggcataatc aatcaccagt tgcagataag taatggattc ctcaaccgag
     4801 agttttttac gggcatcatc accgtggcaa gggaaatcaa aggcaagggc agcgtagcct
     4861 ttatattttg agctaaggcg ttccccaaat ttggtattcg atttcagatc tttagacgaa
     4921 ccaaagccgt gcaacacaat cactacattc tcgatctgct gcaattcttt ttcataaaac
     4981 agtttacagc gaatgctgag accctgttca ttaatctcaa agcgtttttc cattttatgt
     5041 taagtattga aattgaagat gacaaaattt tagcactatt tgagtgcaag cggtcggatc
     5101 tctcgcaaaa tttgcaaatt ttccggtaaa tttaaccgct tgtactagcc aaaatttaag
     5161 catatttata gaatacagcc ttcgttttta aaaggagaaa tgaaatgtta caacttattt
     5221 tagcgatttt atgtagcgtg actgtaggcg tgttgattaa attcgcccgc caaaaaggga
     5281 ttcgtatcga ccaaagtatt gcggtgaact atatcgtgac taccacgatg acgtttttat
     5341 tactaaaacc ggattaccaa gggcagtcga ttaccgatat tattgctaat aacccgactt
     5401 cctatatttt tgtgatttta agtatcacat tgcctactat tttcttggtg caagcaaaag
     5461 ccctagagtt tgccggtatt atccgaaccg atgcggcaca acgtttatcg ctgttcctgc
     5521 cgattttggc ggcattcacc atttttggtg agcaggttac gcaaaataaa ttaattgcac
     5581 ttctgttagc tttcgtggca ctcggttgct tattatggaa aggcaatcag ggaatggaaa
     5641 aaggcggaaa aattgcgatt atcagccttg ctctggtttg ggtaggctat ggcgtgaacg
     5701 atattttatt taaacagatt gccaaatcag gctctgcttt ccctgtcacc ctttcgattg
     5761 cctttatcgg tgcaggctgt tttatgttta tctatttgct acttaaacgc actcaatggc
     5821 acgttccaag tgtcttaacc ggcttattgc taggcgtgtt gaacttcggc aatattctgt
     5881 tctatattca agctcaccaa gccatgaaag atgacccaac tttagtcttc actggaatga
     5941 acttaggggt aatttgctta ggcactttcg ttggagcatt cttctttaaa gagaaaatcc
     6001 acaaagtgaa ctacttaggc gtggcgattg cgattgtggc gattatttgc ttgttttatt
     6061 ggcggtaaat atactttcca aaattaaggg cgaacataat gttcgccctt aatttttgag
     6121 taatatagtg taaaatattc aataaattcg accgcttgtt gtaccgattc atatttcata
     6181 aaggacaaaa aatgaagcta aaagccttat ctacactcat tgcctcttca atgctatata
     6241 gtcagagttt tgctgtaccg ccggaaaaaa cagctgaaga gccacagccc tttttcaata
     6301 aaaccgaatc tgggtatgat ttagtcttta atgatcaaaa ctatcgttta attgaaacgg
     6361 aattaaatgg cgagaaaatc caattccgtg cctttgagcg gattgtgtat gtgaacaatc
     6421 cgattgagcc agattaccaa acaattaatc tgtatgtgcc ggaagcttac tacaataatg
     6481 gcgaaatcaa tggctaccaa gcggataccg ccccgatctt cttgccaaat tcagtggggg
     6541 gctatatgcc ggcaaaagcg gcaagttatg ataaaaaaag acgtgacggt tcaccaggta
     6601 cgattttggt ggcgttatct aaaggctatg tggtggcaag tgtgggagca agaggacgca
     6661 cgctacaaaa agagggaaaa tacaccggca aagcaccttc tgccattatt gattcaaaat
     6721 ccgccgccca taaagcgtgt cggttcgagt ccgaccactg gcaccactca ctatgtaaaa
     6781 tttagtttta catagtttgc tgtaagcatt tgaattttga atcgtttgtg taggattgtt
     6841 caaatcatat tcgcaaatac acagttacta cgatgtatcc agccaataca tccattggga
     6901 ttttccgtcc gaatttcaga cgttctagat cttacacatt ctttcaagtt tgtaaagcct
     6961 gaaaatcatt ttttagattg attttatttg aaaaaatcta ttttttcttt taattcaaat
     7021 ttagt
//
LOCUS       DANLRK010000135         6939 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.135, whole genome shotgun sequence.
ACCESSION   DANLRK010000135 DANLRK010000000
VERSION     DANLRK010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6939
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.135"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(<1..509)
                     /gene="hemG"
                     /locus_tag="PXA33_001991"
     CDS             complement(<1..509)
                     /gene="hemG"
                     /locus_tag="PXA33_001991"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005613176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HDL5121297.1"
                     /translation="MKTLILYFTTDGQTKKIAERLADVITHEVELISLKDQAVDFAEK
                     IANADQIVIGASIRYGHFNPLVYQFVEQHYPALNAKKTAFYGVNLTARKDNRNTPETN
                     TYVRKFLAKVKWKPTHIEVIAGALLYLRYKPFDRLMIRFIMKITKGETDTSKEYEYTD
                     WQQVENFGK"
     gene            complement(510..1994)
                     /locus_tag="PXA33_001992"
     CDS             complement(510..1994)
                     /locus_tag="PXA33_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter"
                     /protein_id="HDL5121298.1"
                     /translation="MQFFSIIRIVGILVMCFSFTMLVPATVALIYGDGGGRAFLEAFA
                     LNFMVGTLLWWLCRDNKNELRSREGFLIVVLFWVVLGLLGAVPFIILENPDLNVAESI
                     FESFSGLTTTGATVITGLDSLPKAILFYRQFLQWLGGMGIIVLAVAIIPLLGIGGMQL
                     YRAEMPGPLKEQKMRPRIAETAKALWLIYLSLTILCAVAFWLAGMTVFDAISHSFSTV
                     SIGGFSTHDASIGYFNNAAISYITVFFLLLSACNFALHFAVIDRIRDAQSRKKGGILS
                     RLYWGDYEFRFFLMVQLGLFLVCTLVLVGYGYFDNTSVTIEQALFQSVSISTTAGFTT
                     NDFSQWSSFLPTLLVLSSFIGGCAGSTGGGLKVIRVLLLYLQSKREIHRYIHPNVIQP
                     IKLGNHVLSERVVDGIWAFFSAYFFVFVICWLATIACGMETFDALNAVIASLNNLGPA
                     LGSVSSNFTQVPDSAKLVLTFAMVCGRLEVFTLLVILSPVFWKD"
     gene            complement(1998..2609)
                     /locus_tag="PXA33_001993"
     CDS             complement(1998..2609)
                     /locus_tag="PXA33_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HDL5121299.1"
                     /translation="MEFFIPKADTIFEEEIKKSRFITYLRHTEGMEQAKAFWQELKIQ
                     HPHARHWCWATVAGLPNDSQQYGFSDDGEPSGTAGKPMLNYLLGSGLGEITAVVVRYY
                     GGIQLGTGGLVKAYGNGVQQALLQLERIRKVLRKNYRLQCEYEQFNTISHLISEKDIV
                     IVEQLFSDKVDLLLAFNPSEVEAFADELTQRFSGKLALIKEEI"
     gene            2724..3677
                     /gene="fmt"
                     /locus_tag="PXA33_001994"
     CDS             2724..3677
                     /gene="fmt"
                     /locus_tag="PXA33_001994"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HDL5121300.1"
                     /translation="MSKLNIIFAGTPDFAAQHLQALLDSEHNVIAVYTQPDKPAGRGK
                     KLQASPVKQLAEAHNIPVYQPKSLRKEEAQAELKALNADVMVVVAYGLILPEAVLNAP
                     KYGCLNVHGSLLPRWRGAAPIQRSIWAGDTETGVTIMLMDVGLDTGDMLHKVTTPIEP
                     NETSASLYAKLAELAPLALLEVLNGLANHTFKAEKQDEALSNYAEKLSKEEAKLDWQL
                     PAVQLERNIRAFNPWPFSFLTLTVDGVEQSIKIHQATVLPHQEKAAGTVINVDKTGIQ
                     IATQQGVLNITQLQPAGKKPMSVQDFLNGRADWFVSGTQLS"
     gene            3795..5168
                     /gene="modF"
                     /locus_tag="PXA33_001995"
     CDS             3795..5168
                     /gene="modF"
                     /locus_tag="PXA33_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HDL5121301.1"
                     /translation="MVVDGNGSGKTTFSLALQGKISLYSGDYGNSFQHIQLLSFEQQQ
                     KMLEQIFKDRNNDSVSPDDFGLTARQVILNGSPKEALCEEYAHKLHIQHLLERPFMHL
                     STGESRKVLFCQMLVSEPDLFILDEPFEGLDQQSVQYWQQLIAELQSKMALVLIVNRF
                     NDIPDAANHIELLDNLSLILQGERAEIEQQAVYSQLKFAEENVNVPLPDSAAPLLSLP
                     ENINPFELENVNIQYGEKKILDNLSWTVQPKQHWWIKGPNGAGKSTLLSIITGDHPQS
                     YANKVRLFGKQRGSGETIWDIKKNIGYVSSQLHMDYRVNCSAMEVILSGFFDSIGVYQ
                     QVPNALQLKAMEWLERLNLSNVAKKPFRSLSWGQQRLLLITRAMVKHPSILILDEPLQ
                     GLDGINRKLVKQFIEQLVTNSQTQLLFVSHQDSDAPNCITHLFEFVPKGETNEFGFEY
                     HQTALGA"
     gene            5169..5759
                     /locus_tag="PXA33_001996"
     CDS             5169..5759
                     /locus_tag="PXA33_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein phosphodiesterase"
                     /protein_id="HDL5121302.1"
                     /translation="MNFLTHSLISLEIDTQTKQQTLYGNFAGDFYKGLIPHLNLADNL
                     KSGVVLHRSIDKISDRPDSLTTQLLQGTFGRYKGIVSDIMIDHFLAKHFQRLFAKDLN
                     EVQAQIFQQIEQQRTIFPEQFVPMFNWLQSRKALTHYADLDFLEQVFLGMSSRVKKGE
                     ILRNAVTEIWQNYTLLEEAAIQEFFTVKDRATRELS"
     gene            5848..6888
                     /locus_tag="PXA33_001997"
     CDS             5848..6888
                     /locus_tag="PXA33_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDL5121303.1"
                     /translation="MQTTLSPQQIAKKHRTIEKKRSFLLLFFLLICLLSLIFDIMTGP
                     SLLPFNEVWKALFQLGEIEETTKVIVYELRLPMAIMALVVGAALGVGGAEIQTLLNNP
                     MASPYTLGLAAAAGFGASIVIAFGGFGLPTEISVPVGAFVMTLFSSAILFAFSSLGRF
                     SSAILVLVGIALLFLFQSLLSLVQFISAPEVSQQILFWLFGNLTKATWQTISIAFGVT
                     TVCILLLFKDAWKLTALRLGEARALSLGINLTWLRMKTLLIVSVMTATCISFVGIIGF
                     IGLVAPHIARMLLGEDQRFFLPGSMLVGAAFLSMSSVLSKSLVPGALFPVGIITALVG
                     VQFFFWIVLAKR"
BASE COUNT         2036 a         1576 c         1432 g         1895 t
ORIGIN      
        1 aatttgccaa aattctccac ctgctgccaa tcggtgtact catattcttt ggaggtatcg
       61 gtttcgcctt tggtaatttt cataataaag cgaatcatca atcggtcgaa tggcttatag
      121 cgtaggtaaa gtaatgcccc agcaattacc tcaatgtggg ttggtttcca tttcacttta
      181 gctaaaaatt tacgcacata agtattggtt tcaggcgtgt ttcgattatc tttgcgagcg
      241 gtcaaattca caccgtaaaa ggcagttttc ttggcgttta atgccggata atgctgttcc
      301 acaaactgat aaaccaacgg gttaaaatgc ccatagcgaa ttgaagcacc gatgacaatt
      361 tggtcggcat ttgcaatttt ttcggcaaaa tcgaccgctt gatcttttag cgaaatcagc
      421 tcgacttcgt gagtaatcac gtccgccaat cgttcggcaa tttttttggt ttgaccatcg
      481 gtcgtaaaat agagaattaa ggttttcatt taatctttcc aaaaaacagg gcttaaaatc
      541 accagcaagg taaacacttc cagacgtccg cataccattg caaaagtcag cactaacttg
      601 gcactgtccg gcacttgggt gaaattgctg ctcacgctcc ctaatgcagg acctagatta
      661 tttagtgagg caattaccgc atttagagca tcaaaggttt ccattccaca agcgatggta
      721 gcaagccagc agatcacgaa cacgaaaaag tacgccgaga aaaacgccca aataccatca
      781 accacccgtt ccgataaaac gtggttgcct aatttaatcg gttgaatcac attggggtga
      841 atgtagcggt ggatttcacg tttactttgt aaataaagca gcaacacccg aatcactttc
      901 aagccgccac cggttgagcc ggcacaacca ccgataaatg aagacagcac caacagcgtg
      961 ggtaaaaacg aactccattg gctaaaatca ttggtagtaa agccggccgt ggtggaaatg
     1021 gacaccgatt gaaacaacgc ttgctcaatc gtaacagagg tattatcgaa ataaccgtaa
     1081 cccactaaca ccaaggtaca aaccaagaaa agccctaact gcaccatcaa gaaaaaacga
     1141 aactcgtaat cgccccaata caagcggctt aaaatacccc cttttttgcg tgattgtgca
     1201 tctcgtatgc gatcaatcac cgcaaaatgc aatgcaaagt tgcaggcaga aagtagcagg
     1261 aaaaacacgg taatatagct aattgccgca ttattaaaat agccgatgct ggcatcgtgg
     1321 gtagaaaatc cgccaatcga aaccgtggaa aagctatgcg aaatggcatc aaacaccgtc
     1381 attccggcga gccaaaatgc gaccgcacac agaatcgtca ggctgagata aatcagccac
     1441 aacgcttttg ccgtttccgc aatgcgaggt cgcattttct gctctttgag cggtccgggc
     1501 atttccgcac gataaagttg cattccacca atgcctaaca gcggaataat cgcaaccgct
     1561 aacacaatga tccccattcc ccctagccat tgcaaaaatt ggcggtagaa taaaatcgct
     1621 ttgggcaagc tatctaagcc ggtgatgacg gttgcacccg ttgtagttaa gccggaaaag
     1681 gattcaaaaa tggattccgc cacgtttaaa tcggggtttt ccaaaataat aaaaggcaca
     1741 gccccgagca aacccagcac cacccaaaac agtactacaa tcaaaaagcc ctcacgggaa
     1801 cgtagctcgt ttttgttgtc acggcacagc caccaaagca gcgtaccgac cataaaattc
     1861 agagcgaagg cttctaaaaa cgcccgccca ccgccatcgc cataaattag ggcgaccgtt
     1921 gccggcacaa gcattgtgaa tgaaaaacac atcaccaaaa tgccaacaat acgaataatg
     1981 gaaaaaaatt gcatagacta gatttcttct tttattaatg cgagcttgcc cgaaaagcgt
     2041 tgggttaatt catctgcaaa ggcctcaacc tcgctcgggt taaatgccag caacaaatcc
     2101 actttatcgc taaaaagttg ctcgacaatc acaatatctt tctcgctaat gagatgagaa
     2161 atggtattaa actgctcata ttcacattgc aagcggtagt tttttcgcaa aactttgcga
     2221 atccgctcca actgcaataa cgcctgttgc accccattgc cataggcttt caccaagccg
     2281 ccggtgccaa gctggatacc accgtagtag cgaaccacca ccgctgtaat ttcccccaag
     2341 cctgacccaa gcaaataatt gagcattggc ttacctgctg taccagacgg ttctccatca
     2401 tccgaaaagc cgtattgttg cgaatcattc ggcaagcctg ccaccgttgc ccaacaccaa
     2461 tggcgtgcgt gcggatgctg aattttcagc tcctgccaaa atgccttggc ctgctccata
     2521 ccctcagtat ggcgaaggta ggtgataaat cggctttttt taatctcttc ttcaaaaatt
     2581 gtatccgctt tggggataaa aaattccatt gtttgattct cttatcttga atgtggcaat
     2641 gataccatat tcaagccact ttttatttgt gctgtttaat ggtttaataa gtctttcatt
     2701 gataacttga acgaataaaa aatatgtcaa aactcaatat tatttttgcc ggcacgcctg
     2761 attttgccgc tcaacattta caagccttat tagattccga gcataacgtg attgcggttt
     2821 atactcagcc ggataaacct gccgggcgag gcaaaaaact gcaagccagc ccggtaaaac
     2881 agctggccga agctcacaac attcctgttt accaacccaa atccctgcgt aaggaagagg
     2941 ctcaagctga actgaaagcc ctaaatgccg atgtgatggt ggtggtagct tacggcttaa
     3001 ttctacctga agcagtattg aatgctccta aatatggttg cctaaacgta cacggttcgc
     3061 tactacctcg ctggcgtgga gcagctccga ttcagcgttc gatttgggca ggtgatacag
     3121 aaaccggcgt aaccattatg ctaatggacg ttggcttaga caccggtgat atgttacata
     3181 aagtcacaac cccgattgag ccgaatgaaa cctccgcctc cttgtatgcc aaactggcag
     3241 aacttgcccc tctggcattg cttgaggtgt taaacggttt agccaaccac acattcaaag
     3301 cggaaaaaca agatgaagca ctgtccaatt acgccgagaa actttccaaa gaagaagcta
     3361 aattggattg gcaactccca gcagttcaac tggaacgcaa tatccgagct tttaaccctt
     3421 ggccgtttag ttttttaacc ttaaccgttg atggcgttga gcaaagtatc aaaattcacc
     3481 aagccacggt gttacctcac caagaaaaag cagccggcac ggtaattaat gtggataaaa
     3541 ccgggattca aattgccacc caacagggcg tgctgaatat tacccaactt caaccggcgg
     3601 gtaaaaaacc aatgtcggtg caagatttct taaacggtcg agcggattgg ttcgtcagcg
     3661 ggacacaact aagttaaaac gggaaatata tgcaaacaca gccttcactt acaataacca
     3721 atgcccgatt ttcactggtt aaacaacata aactcacgat tgacgcctta cagatccaac
     3781 ccaatgactt ttaggtggtc gtagacggaa acggttcagg caaaacaact ttttcactcg
     3841 ctttacaggg taaaatctcg ctctattccg gcgactatgg taattcgttc caacatattc
     3901 agctcctctc tttcgagcaa caacaaaaaa tgttggagca gattttcaaa gatcggaaca
     3961 acgatagtgt ctcgcccgat gatttcggct taaccgcacg acaagtaatt ttaaatggca
     4021 gtccaaaaga ggcgttatgt gaggaatatg cccacaaatt gcatattcaa catctgcttg
     4081 aacgcccgtt tatgcacctt tccaccggtg aaagccgcaa agtgctattt tgccaaatgt
     4141 tagtgagcga gccggattta ttcattttag acgaaccgtt tgaagggctg gatcagcagt
     4201 cggtgcaata ttggcaacaa ctgattgccg aactacaaag caaaatggcg ttagtgctga
     4261 ttgtaaatcg ttttaacgat attcctgatg ctgccaatca tattgagtta ttagataatt
     4321 tatcattaat tctgcaagga gagcgagcag aaattgaaca acaagcggtc tattctcagc
     4381 taaaatttgc agaagagaat gtcaatgtgc cactgcctga tagtgcagct ccgctactca
     4441 gcctgccgga aaacatcaat ccgtttgagt tggaaaatgt gaatattcaa tatggggaga
     4501 agaagatttt agataaccta agttggacgg ttcagcctaa acaacactgg tggattaaag
     4561 gcccgaatgg agctgggaaa tcgaccttgc tttcgattat caccggcgat cacccacaat
     4621 cttatgccaa taaagtccga ctatttggca aacaacgggg aagtggcgaa accatttggg
     4681 atattaagaa aaatatcggc tatgtcagca gccagttgca tatggattat cgggtaaatt
     4741 gctccgcaat ggaggtcatt ctttctggct tttttgacag catcggcgtg tatcaacaag
     4801 tgccgaatgc cttgcaatta aaagcaatgg aatggttgga acgcctcaat ttaagcaatg
     4861 ttgccaaaaa gccgtttcgc tctctctcgt gggggcaaca acgcttactt ttgattaccc
     4921 gagcaatggt gaaacaccca tcaattctga ttctagacga accgttacaa ggtttagacg
     4981 gcattaaccg caaattagtc aaacagttca ttgaacaatt agtgactaac agccaaacgc
     5041 aactactttt tgtttcgcac caagacagcg atgctccaaa ttgcattacg catttatttg
     5101 agtttgtacc gaaaggcgaa accaacgagt ttgggtttga gtatcaccaa actgcacttg
     5161 gagcataaat gaattttctt acccattcgc tgatttcact tgaaattgac acccaaacca
     5221 agcaacagac cctatatggc aactttgccg gtgatttcta taaaggttta attccgcatc
     5281 tcaatttagc ggataactta aaaagcggtg tggtgttaca ccgctcgatt gataagattt
     5341 cggaccgacc ggacagcctc acaacccaat tactccaagg cacattcggg cgttataaag
     5401 gcattgtgtc ggatattatg atcgaccatt ttctcgctaa acatttccaa cggttatttg
     5461 ctaaagattt aaatgaggta caagcacaga tctttcagca aatcgaacaa caacgcacga
     5521 tttttcctga acaatttgtg ccgatgttta actggttaca gtcacgaaaa gcattaactc
     5581 attatgccga tttagatttc ttagaacagg tattcttggg aatgtcatca agagttaaga
     5641 aaggtgagat tttaagaaat gctgtaaccg aaatttggca gaattatact cttttagaag
     5701 aagccgctat tcaggagttt tttactgtaa aagacagggc tactcgagaa cttagttgaa
     5761 tactttagtc ggtatcttgg atattcttga atttatcgat cttttctctt atcctgtctg
     5821 ataataattc tcaaacatta catttttatg caaacaacgc tctctcccca acaaatagct
     5881 aaaaagcacc gaactattga aaagaaacgc agttttttat tactcttttt cttactcatt
     5941 tgtttactca gcctcatttt cgatattatg acaggccctt ccctgcttcc gtttaatgaa
     6001 gtttggaaag ccttattcca attaggcgaa attgaagaaa ccaccaaagt gattgtctat
     6061 gagctacgct taccaatggc aataatggca ttggtggttg gagcagcact tggtgttggc
     6121 ggagctgaaa ttcaaacttt gcttaataat ccaatggcaa gcccttatac attgggttta
     6181 gcagctgcag ccggctttgg tgcttcgatt gtgattgcct ttggtggatt tggtttaccc
     6241 accgaaattt ctgtacctgt cggggctttt gtgatgacac tattttcttc cgccatttta
     6301 tttgcctttt cctctttagg acgttttagc tcggcaatat tagtgctggt aggaattgcc
     6361 ttattgtttt tatttcagtc tttactgtca ttggtgcaat ttatttctgc acctgaggta
     6421 tcccaacaaa ttctgttttg gctattcggt aatttaacta aagctacttg gcagactatc
     6481 tcaattgcct ttggcgtaac tacagtctgt attttattat tattcaaaga tgcgtggaaa
     6541 ttaactgcct tgcgtttggg cgaagctcga gcattgagct taggcattaa cttaacttgg
     6601 ttacgaatga aaacattgct gattgtgtct gtaatgacgg ccacctgcat tagctttgta
     6661 ggcattatcg gctttattgg cttagttgcc ccgcatattg cccgaatgtt gctaggcgaa
     6721 gaccaacgct ttttcctgcc cggttcaatg ttagtgggtg cggcattttt atcgatgtca
     6781 tccgtgctat caaaaagcct tgtgccgggc gcattatttc cggtcggtat tattacggca
     6841 ttagtcggtg tgcagttctt tttctggatt gtattggcga aacgataagg attttctatg
     6901 ctcaacttaa acgatgtaac tattaagcgt ggaaactta
//
LOCUS       DANLRK010000136         6937 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.136, whole genome shotgun sequence.
ACCESSION   DANLRK010000136 DANLRK010000000
VERSION     DANLRK010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6937
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.136"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(305..2653)
                     /locus_tag="PXA33_001998"
     CDS             complement(305..2653)
                     /locus_tag="PXA33_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005612269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HDL5121304.1"
                     /translation="MLISAQFSPLVWAENNDVAVLDEVSVVGSTPSISQGSEVTLLKV
                     SDKIIAGKEFKKRSATLGNALASELGVHSNPFGGGASKPIIRGQEGARIRILQNGSDV
                     IDMSNLSPDHAVVADSLLADQVEILRGSSTLLYASSSPVGIVNVVDKRIPTAIPEKGY
                     EVELNSRFDTAAKEKVGALGATFGIGNHIAVRVEGLSRHSDNYRVPGINLGERLNYVP
                     DTYNKSKVGTLGLSFVGERGYIGASYSKRRDNYGLPGHNHKFDFCTGHIYGNKRDKYA
                     YTYLYPHLIGEENIGSNPHFHCGTNHAEDGTHSHDNPFGHAHDHTHKGPWVDLESKRI
                     DVKAELRQPFKGVDKIKASYADADYYHDEKDAGVLATRYHKQLKKDQDYGKPVNIFKN
                     RGKNTRLEVYHAPLGGLTGVWGVQYQTQKSSMNAPKDREVKFPLVENTNKQFSLFGVE
                     QYMWDSVAVELAGRMEKQKIEIEYDRNEIKRLQEHYRISGGKQVEPDLSPYDETAYAY
                     SGTLNWFFHPDYQLSFTASHNERLPTPMELYYHGQHIATNSFEYGNKDLKKEQSNNVE
                     LGLGYQAERIGYKVSVYYNHFKNYIYNENLFRENQLFMRRYNQAKARFYGVEAEASYR
                     FNDQYQATIFGDMVRGWLTNLPPLKINSDYSVFKDYLPENVEKGKDYLLYRADQNTPR
                     TPPMRLGFRFNAEFTPNWSGDLELIRTFTQRRTSQLEYITEGNTMLNVGLSYSNKWKE
                     LDYKISLNGTNLLNQPVYIHTSYHQFVPQMGRNFMLGMEMKF"
     gene            2838..4295
                     /locus_tag="PXA33_001999"
     CDS             2838..4295
                     /locus_tag="PXA33_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121305.1"
                     /translation="MRSTFKLTLAALAVSIVLSGCKSSQGPSIVADSPPPKEITATDG
                     NDKEKTDNKEKKSAQPNKSDPQPDKPANAKDPVKPDAETNKPSKKDKEYTPPATEKID
                     NSSVQPDKMVPNPIENKENDSNKPNPKESDSDNNDKVTEGNKPNAPTETVNKDKEAEK
                     SKKENKEDSSNNTPNINFDGAKPIDTDSKGSPYRVINLNPRKSVVNASAMQKVDFDSF
                     TDESVENSPFVSSDTEKEYNNTLVFGLGAGDKDSYFKNLNQKGDKHLGIHSGVYEDAN
                     LEGNTKKINYLYVNQPYSSYGALFTDQKDSHLFNVRLTAGRDDVKENGDSYAEYGVFT
                     QSGKEAKWNDNLIGDATYKGQAIARVIRQVNGEQVAGIPQFDGDVTLNLHLSKEWNES
                     KLNGKIDSQSVGVITLKESEIANPTALRSNIGFGEEVIVENKENLVGDYAVDFTGPQL
                     EEAVGVVELENEAETFSENEVIKYNAVFGVSKAAK"
     gene            4359..5771
                     /locus_tag="PXA33_002000"
     CDS             4359..5771
                     /locus_tag="PXA33_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HDL5121306.1"
                     /translation="MKKIALLALFPMMLFARDPNTTQAYIEHTAQPTTTPQRVKIEKS
                     VPIFNETELRANQLLTEKLLIQAIYSRRLANMEKLTAIYRDFTHIDPVLLRYAQGKIA
                     LLKGDYAQAIQHFQTILAKQPKLNTVRIELAIALLYDQQLNQAQAQFEKAKSAEHLPP
                     KVHALIEQYQQHLAKKQEWKFSFSANYVRDGNINRTSKQPNIEQTGYVKQPNLLPQTA
                     HGVHYAFGLQRDFNLFDSHYVSFDNQTQGELYWDNHDYDEITNRTLFGYAYKKSDQTT
                     RLLPFYEKRWKHHRSERWTNGIRLEHAQWFSPHWQLATALEYGKKRYFDLPIYNGSYK
                     LASATLLWLRNPQQFFFVGTDFNQDKTQLKQYSSDTKGIRFGWQREWQALELSSRLTL
                     GYTDRRYKDTAILGGKLNLGRIRHDRVQSANLTLWKRDWHWLGLMPKLQFSYAKHRSN
                     LPSLYAYTDKNINLIIESRF"
     gene            complement(6021..6344)
                     /locus_tag="PXA33_002001"
     CDS             complement(6021..6344)
                     /locus_tag="PXA33_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin"
                     /protein_id="HDL5121307.1"
                     /translation="MQKAYQIYTHSEREKVTEAKLELGKVETRLRNEGKTKEEIAQHP
                     EYLQLKRDLNDKQQTFDNAYGTGSKTKRAVDAVTAALQGLAAKDSGQAAVGLASPYLN
                     EQIKK"
     gene            complement(6313..6840)
                     /locus_tag="PXA33_002002"
     CDS             complement(6313..6840)
                     /locus_tag="PXA33_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HDL5121308.1"
                     /translation="MRDKNKERQQTLERYQELYDQDPELRVIAANSEGDLDLLQETFF
                     VKGELTITDSTPKEKWNWLHMCNLNSYSPSPLSVVEFYIKHGVDVNAQDCYGMTPLHY
                     AMRAKNVDAAIALLNAGADPNIENKDQVIPLSMIGAVPKEIELIEHDISEVKETQELV
                     KGIGEIAEGLSDLHA"
BASE COUNT         2104 a         1527 c         1306 g         2000 t
ORIGIN      
        1 tcattaaagt aaacacaaga tagtgtgctt aaagcaaaac atcaaccaat aaatttttac
       61 tttttctctt gacagatgct aagttattta taaataacca tatttttaat aaagggataa
      121 agttaattgt cttatttaac aacaaacagg aaattttggt aagctccgaa aataacaaga
      181 aaggaaaaac agcactattt tatccaaatt accaatacct gagttttaaa caagcggtta
      241 tttttagccg tttttttgca aaatttgaag aaaaaataac cgcttgtctt gttatgtcga
      301 gaacttaaaa tttcatctcc attcccaaca taaaatttcg tcccatttgt ggtacaaatt
      361 ggtgatacga agtgtggata tacaccggct gattcagtaa gtttgtgccg tttagactga
      421 ttttgtaatc cagctctttc cacttattgc tatacgacaa cccgacattc aacatcgtat
      481 taccttcggt aatatattcc aactggctcg tacggcgttg ggtaaaggtg cggatcagct
      541 ctaaatcccc cgaccaattt ggggtaaatt ccgcattaaa gcgaaaacct aaacgcattg
      601 gcggtgtacg aggggtgttt tggtcagccc gatatagaag ataatctttc cctttttcca
      661 cattttccgg cagataatct ttgaacacac tataatcgct atttatcttc aatggtggta
      721 aatttgttag ccagcctcgt accatatcgc cgaaaatcgt ggcttggtat tggtcgttaa
      781 agcggtagct tgcctctgct tctaccccgt aaaaacgggc ttttgcctga ttataacggc
      841 gcataaatag ctgattttca cggaaaagat tttcattgta gatgtagttt ttaaagtgat
      901 tgtaataaac actcacttta tagccaatac gttcagcttg atagcccaag cccaactcca
      961 cattatttga ctgctctttt ttcaagtctt tattgccgta ttcaaacgaa ttagtagcaa
     1021 tatgctgccc gtgataatac agctccattg gcgttggcaa gcgttcattg tgcgaggcag
     1081 taaacgaaag ctgatagtca gggtggaaga accagttaag cgtgccggaa taggcataag
     1141 ccgtttcatc atagggtgaa agatctggct caacctgttt gccgccactg atacggtagt
     1201 gttcttgtaa ccgtttgatt tcattgcgat catactcaat ctcaatcttc tgcttttcca
     1261 ttcgtccggc aagctcaaca gcaacgctat cccacatata ctgctcaacc ccaaacaggc
     1321 tgaactgttt attggtattt tcaaccagcg ggaatttcac ttcgcgatct ttcggcgcat
     1381 tcatactgct tttttgcgtt tgatactgaa caccccatac accggttaag ccacctagcg
     1441 gtgcgtgata gacctccaag cgggtatttt tgccccgatt tttgaaaata ttcaccggct
     1501 tgccgtaatc ctgatctttt ttcagctgtt tatggtaacg ggtggcaagc acgcctgcat
     1561 ctttttcatc gtggtaataa tcggcatcag catagcttgc cttaatttta tctacgccct
     1621 taaacggctg gcgtagctcg gctttcacat caatccgttt tgattctaaa tccacccacg
     1681 ggcctttgtg agtgtgatcg tgggcgtgtc cgaacgggtt atcgtggctg tgtgtgccgt
     1741 cctcggcgtg gttggttcca cagtggaagt gcggattgct cccaatattt tcctcaccaa
     1801 tcaaatgcgg atagagatag gtataagcgt atttatcccg cttattaccg tagatatgac
     1861 cggtacagaa atcaaattta tggttatgcc ccggcaagcc ataattatcc cgccgtttgc
     1921 tgtatgaggc accaatatag cctcgttcac cgacaaagga taagcccaac gtgccgactt
     1981 tggatttatt gtaagtgtcc ggcacatagt ttaagcgctc gcctaagttg atccccggca
     2041 cacggtagtt atcactatga cgactcagcc cttcgactcg caccgcaatg tggttaccga
     2101 tgccaaaggt tgcccctaat gcgccgactt tctctttggc ggcggtatca aaacggctat
     2161 tgagttcaac ctcatatccc ttttcaggaa tagcggtcgg aatacgttta tccaccacat
     2221 ttacaattcc taccggcgaa ctgctggcat aaagcaaggt gctggaacca cgcaagattt
     2281 cgacctgatc ggcaagcagt gaatcagcaa caacggcgtg atcaggagat aggttcgaca
     2341 tatcaatcac gtctgaaccg ttttgcaaaa tgcgaattct cgccccttcc tgcccacgaa
     2401 taatcggctt actcgctccg ccaccaaacg ggttactatg cacgcctaat tctgacgcca
     2461 acgcattacc cagcgttgcc gaacgctttt taaattcctt acctgcaata attttatcac
     2521 tgacttttag caaggtaact tcactcccct gcgatatact tggagtgctg ccaacgaccg
     2581 aaacttcatc taacaccgct acatcgttat tttccgccca aacaagcggg ctaaattgag
     2641 ccgaaatcag caatgccaat cctgttttct taaacacaat ctttctcctt aaaaaaatta
     2701 aggataataa ggtactgcaa aatatgagtc tttacaataa taaccataaa aaaattatat
     2761 tttttaaatt ttccctattg ccaaacagga aatatatcca ttaaaatttt atggatttaa
     2821 tctgacggag acaaaatatg cgttctacat tcaaacttac tcttgcagct ttagctgttt
     2881 ctattgtttt aagtggctgt aaatctagcc aaggtccatc tattgttgca gacagccctc
     2941 cacctaagga aataacagct actgatggca acgataaaga gaaaaccgat aacaaagaga
     3001 aaaagtctgc gcaaccaaat aaatccgatc ctcaaccgga taaacctgct aacgcaaaag
     3061 acccagtaaa acctgatgcg gagacaaata aaccaagtaa aaaagataaa gaatatacac
     3121 cacctgctac tgaaaaaatc gataactcat cagtacagcc ggataaaatg gtgcctaatc
     3181 ctatcgagaa taaggaaaac gacagtaata aacctaaccc caaagaaagc gatagcgata
     3241 ataatgacaa agtcactgaa ggcaataaac caaatgcccc aacagaaaca gtaaataaag
     3301 ataaagaagc tgagaaaagt aaaaaggaga ataaagagga ctcaagcaat aatactccta
     3361 acattaattt tgacggggca aagcctatag atacagatag taaaggtagc ccgtaccgag
     3421 taataaacct aaatccgaga aaaagcgtag taaatgcaag tgctatgcaa aaagtagatt
     3481 tcgactcttt tacggatgaa tctgttgaaa attcaccatt tgtcagtagc gatactgaaa
     3541 aagaatacaa taatacactt gtttttgggc ttggtgccgg agataaagat tcttatttta
     3601 agaacttaaa tcaaaaaggg gataaacatt taggtattca tagtggcgtg tatgaagatg
     3661 cgaatttaga aggtaatact aaaaaaatta actatctcta tgtaaatcaa ccttattcga
     3721 gctatggtgc tttatttacg gaccaaaaag acagccattt attcaacgtt cgtctgaccg
     3781 ctggtagaga tgatgtaaaa gaaaatggag atagttatgc cgaatatggt gtattcacac
     3841 aatcaggaaa agaagctaaa tggaatgata acctgattgg tgatgccact tacaaaggtc
     3901 aagctattgc tcgcgttatt cgtcaggtaa atggggaaca agtcgcaggt attcctcaat
     3961 ttgatggtga tgttacatta aatttacacc tttccaaaga atggaatgaa agtaaactaa
     4021 acggcaaaat tgatagccaa agtgttgggg taattacact taaagaaagt gaaatagcta
     4081 accctaccgc actccgctca aatattggtt ttggagaaga agtcatagtt gagaataaag
     4141 agaatcttgt gggtgattac gctgtcgatt ttaccgggcc tcagcttgaa gaggcggtag
     4201 gcgttgttga attagaaaac gaagccgaga cattctctga aaatgaagtc attaaataca
     4261 atgcggtatt tggcgtaagc aaagcggcga aatagtattt tttgtatctt acaaagggct
     4321 gttaagccct tttattttta ttattttaag gataaaaaat gaaaaaaatt gccttactgg
     4381 cacttttccc aatgatgtta tttgcccgag atcctaatac tacacaggct tatattgagc
     4441 atactgcaca gccaactaca accccccaaa gggtcaagat cgaaaaatca gtgcctattt
     4501 ttaacgaaac tgaattacgt gctaatcaat tattgaccga aaagctactt atccaagcta
     4561 tttatagtcg taggcttgct aacatggaaa aattaactgc gatttatcgg gattttaccc
     4621 acattgaccc ggttttactg cgttatgcac aaggtaaaat tgccctactg aaaggcgatt
     4681 acgcacaggc aattcagcac ttccaaacca ttcttgccaa acaacccaaa ctaaacactg
     4741 taagaattga gctggcgatt gctctattgt acgatcagca acttaatcaa gcccaagcac
     4801 aatttgaaaa agccaaaagt gccgaacatc taccgcctaa agttcacgca ttaattgagc
     4861 aatatcagca acatctggca aaaaaacagg aatggaaatt tagtttttca gccaattatg
     4921 tgcgagacgg caacattaac cgaacctcga aacagccgaa cattgagcaa accggctatg
     4981 taaaacaacc taatttgttg ccccaaaccg cacatggtgt tcactatgcg tttggattac
     5041 agagagattt caacctgttc gactcacact atgtgagttt tgacaaccaa acccaaggtg
     5101 aactttattg ggataaccac gattatgatg aaattaccaa tcgcaccttg tttggatacg
     5161 cttacaaaaa atccgaccaa accacccgct tgttaccgtt ttatgaaaaa cgctggaaac
     5221 atcaccgttc ggaacgctgg acaaacggaa ttcgacttga acacgcacaa tggttttcac
     5281 cgcattggca actcgccacc gcattggaat acggcaaaaa acgttatttt gatttgccga
     5341 tttataacgg cagctacaaa cttgcctctg ccacattgct ttggttacgc aatccacaac
     5401 aatttttctt tgtcggcact gatttcaatc aagacaaaac tcagttaaaa caatatagct
     5461 ctgatactaa aggcatacgc tttggctggc aacgagaatg gcaagccctc gagctttcca
     5521 gtcgcctcac gcttggctat actgacagac gttacaaaga caccgctata ttgggaggaa
     5581 aacttaatct tggcagaatc cgccatgacc gtgtgcaaag tgctaacctc acgctttgga
     5641 aacgagattg gcactggctc ggcctgatgc cgaagctgca atttagttac gccaaacacc
     5701 gtagcaacct gccgagcctc tatgcctaca ccgataaaaa catcaattta attattgaaa
     5761 gtcggttcta gctccataaa aacaacaagc ggttaaaatt cctagaaatt ttgcaaattg
     5821 caaaaaatgt agaaaattta accgcttgta tctatactgc agcttctttt ccttcagtat
     5881 aaattaatat acaccgttgc aacaataatg gctgctccct gtcatcacct tgaactgaaa
     5941 attgctcaag gtggatatga ctatcgcagc cgcttccacc gcacccagta atgtatgggc
     6001 aagcaggttg gcttgggtat ctacttttta atttgttcgt tgagatatgg cgaagctaag
     6061 cctactgctg cttgtcctga atctttcgcc gctaaacctt gtaaggctgc cgtcaccgca
     6121 tccaccgcac gcttggtttt gctgcccgtg ccgtaggcgt tatcaaaagt ctgctgttta
     6181 tcgtttaaat cccgttttaa ttggagatat tcaggatgtt gagcgatttc ttcttttgtt
     6241 tttccctcat ttctcaaacg ggtttccact ttgcccaatt ccagcttcgc ttccgtcact
     6301 ttttcacgct cactatgcgt gtaaatctga taagccttct gcaatctcgc ctatgccctt
     6361 caccagctcc tgtgtttctt tcacctcact aatatcgtgt tctattaact ctatctcttt
     6421 aggaactgct ccaatcatag acaatggaat aacttgatct ttattttcaa tattcggatc
     6481 tgcccccgca tttaataatg ctatcgctgc atcaacattc tttgccctca ttgcataatg
     6541 taaaggggtc attccgtagc aatcttgggc attaacatct accccatgtt taatgtaaaa
     6601 ttcgactaca gaaagaggag atggtgaata actatttaag ttacacatat gtagccagtt
     6661 ccatttttct tttggtgtgc tatccgtaat agtcagttca ccttttacga aaaatgtttc
     6721 ttgtaaaaga tcgagatcgc cttctgagtt tgctgcaata actcttaatt caggatcttg
     6781 atcatataat tcttgataac gttctaatgt ctgctgacgt tctttattct tatctctcat
     6841 tttactttcc tatttgttgg aattttttca tgtcatcgat tattgattta atattatctt
     6901 tccccggcat ctcattctta tgactccgat tttcaga
//
LOCUS       DANLRK010000137         6843 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.137, whole genome shotgun sequence.
ACCESSION   DANLRK010000137 DANLRK010000000
VERSION     DANLRK010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6843
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.137"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            58..630
                     /locus_tag="PXA33_002003"
     CDS             58..630
                     /locus_tag="PXA33_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDL5121309.1"
                     /translation="MKKNLFATTFKPVFIASILSVFGGYSSIAVASLSSKPATPNQII
                     ASKAERLAKSSSVFTNRANLPTSSAHTANYSGRINSVYHNWVGTRYRLGGTTKAGIDC
                     SAFVREVMGKAFSKDMPRSTAEQRHVGHSISKSDLRPGDLVFFRKNNHVGVYIGNGKF
                     VHASSSRGVTTSSLSESYWSRNYTQSRRVI"
     gene            complement(697..1155)
                     /locus_tag="PXA33_002004"
     CDS             complement(697..1155)
                     /locus_tag="PXA33_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL5121310.1"
                     /translation="MASIRQISDQTAAYQPIPLYRDFNSSRSVKLDLNSLCIKRPRDT
                     FFIHVKNPNLIAWGIELDDLLIVEQTNQYLVNDLLVLEKDGEYKFYQFFNEIQNGEGI
                     AEKILFSLDVSESNLRITDWAEVKIAGVITNVVHQMRTRGAFTQAKQRVA"
     gene            complement(1329..1712)
                     /gene="grcA"
                     /locus_tag="PXA33_002005"
     CDS             complement(1329..1712)
                     /gene="grcA"
                     /locus_tag="PXA33_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HDL5121311.1"
                     /translation="MIKGVQITESSNSNLLNSFWLLDEAKNEARCLAAKAGFEEDQVV
                     AISELGDIAYREVPVNVAPTIKVEGGQHLNVNVLRRETLEDAIQNPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVITRTFTESL"
     gene            1871..2560
                     /gene="ung"
                     /locus_tag="PXA33_002006"
     CDS             1871..2560
                     /gene="ung"
                     /locus_tag="PXA33_002006"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDL5121312.1"
                     /translation="MKTWTEALGEEKQKAYFQQILQYVHNERLAGKVIYPPQNEVFSA
                     FALTEFSDVKVVILGQDPYHGPNQAHGLAFSVKPPVAPPPSLVNMYKELSEDIEGFQI
                     PPHGYLIDWAKQGVFMLNTSLTVEQGRAGSHSQIGWEIFTDKVIAQLNQHRDNLVFLL
                     WGSHAQRKGQFIDRNRHCVLASPHPSPLSAYRGFFGCKHFSKANNYLISQGISPINWQ
                     LSVDVSYSSTH"
     gene            2576..3496
                     /locus_tag="PXA33_002007"
     CDS             2576..3496
                     /locus_tag="PXA33_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HDL5121313.1"
                     /translation="MNFDLPIVSIAFVVLVLVALSSTIKIVPQGYHWTVERFGRYTKT
                     LSPGLNIVVPFIDRIGRKMNMMEQVLDIPSQEVISRDNASVAIDAVCFVQTVDARRAA
                     YEVNHLEQAIVNLTMTNMRTVLGSMDLDDMLSQRDLINGRLLSIVDEATNIWGVKVTR
                     IEIRDVRPPKELVAAMNAQMKAERNKRADILEAEGIRQAEILRAEGEKQSRILKAEGE
                     RQEAFLQAEARERAAEAEAKATQMVSEAIAKGDTTAINYFIAQKYTEALKEIGSSDNS
                     KVVLMPLEAGNLIGSVAGIAEILKSNKTSS"
     gene            3532..3969
                     /locus_tag="PXA33_002008"
     CDS             3532..3969
                     /locus_tag="PXA33_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HDL5121314.1"
                     /translation="MDWFFNWIGWSILGFVLLALELVLPGVFIMWWGFASLILAFIVA
                     LFTLGQALQIALFAIIATIFSLIWWKYQHGKDKKEDQSSSLNSREHAMLGAQGFVVEI
                     LPNGIARGKFGDTTWRVIGNNLQVADKIEVQKVEGITLFVRKQ"
     gene            complement(4110..6746)
                     /locus_tag="PXA33_002009"
     CDS             complement(4110..6746)
                     /locus_tag="PXA33_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HDL5121315.1"
                     /translation="MDEGFMVKHPRHSSHLHPLIHQLTTPEGEVRIYDASYPQFEVNP
                     VEKEDGIDLIPSLETHGAGVAGIIAAQADKTLGDGYSGGIAKGAELYVATKSYKRTLE
                     KVIQDAKKELENAKDEEDEKTPSLDQMAKNDLLASKEKEMAIERAEWASGLNKLLDNN
                     VFAINNSWNPFSISDDINVVDKFYQSIKQNKHNPLLQAIMRAKNSNTLLVFAAGNESK
                     KQPGVMALLPRYFPELEKNLISAVAVDKEQKIASYSNHCGASKNWCVAAPGDLHVLIG
                     VADEHKKPQYGLTKEQGTSFSAPAITASLAVLKERFDYLTATQIRDTLLTTATDLGEK
                     GVDNVYGWGLINLKKAVNGPTQFLNDETITVTRDDHWSNPLASQFKITKKGDKSLHLD
                     GENHLDTVAVEEGRLALNGKTKVKTISNHANLAVNGTEVEQNYSSSGQSQLEVLGKSG
                     LIANAQANIHLAGSLKIDDKLTEKTEAGDVSATVVQLKDKATYQGGFTQLVENENLAK
                     RGLIQDLYFKESEIIAKVNKPLTDEKADTNGQAGLALLNALRTTPIAYRRSWYNGWLQ
                     SALEQRKLDNLHYAVSNNIYADSLELLRSQNAKGLTQAQQHLFTAYHTPLQTTVWAEH
                     LNQKQSASSKHTDVKHHQSQLGVNHKLADKTVLSATLSQQKNRLEKPFAQATLKQTAL
                     NIGLRYHLDNAWFSEATLQFARQKYQQSRRFASHQLGTAETRGSTLGGEMRIGYQFMP
                     NQWIIEPSLGVQWIQTKMNGLNESGELATQTAAMRYRDVNIVPSVKLQRTFQLEQGSI
                     SPYIGLNYLHRLNGKITKITSNIAGKTLHSEATTKRNRQLNGEVGVKLHYKNWFTAMN
                     LDYSRVKSANLFGWKVNVGFSF"
BASE COUNT         1954 a         1266 c         1344 g         2279 t
ORIGIN      
        1 aagatttagt taaggaattt accgctgtaa ttgttctggc gagacgagac ataaattatg
       61 aaaaaaaatc tttttgcaac aaccttcaag cccgtattta ttgcttctat tttgtctgtt
      121 tttggagggt attcttccat tgctgtagca agtttaagtt ctaaaccggc cacaccaaat
      181 caaatcattg caagtaaagc agaaagatta gctaagtctt cttcagtatt taccaatcga
      241 gcaaatcttc caacttcttc cgctcataca gcgaattata gtggtcgtat taatagtgtt
      301 tatcataatt gggtagggac acgctatcgt ttaggcggca caacaaaagc aggaattgat
      361 tgttccgcct ttgtgcgtga agtaatgggc aaagcattta gtaaagatat gcctcgttca
      421 actgcagaac aacgccatgt cgggcattcc atttcaaaat ctgatttacg cccaggtgat
      481 ttagtctttt tccgtaagaa taaccatgta ggggtttata ttggtaatgg taaatttgta
      541 cacgctagtt caagtagagg ggtaaccacc agctcattgt cagaaagcta ctggtcaaga
      601 aactacacac aatctcgccg tgtaatttaa taaattacaa ttccaagatg aaaagaacac
      661 tcacttttgc gagtgttctt ttttaattaa aaaagtttat gctacgcgtt gcttagcttg
      721 tgtaaatgct ccacgagtac gcatttgatg aaccacatta gtaattacac ctgctatttt
      781 tacttcagcc caatcagtaa ttctaagatt tgattcgctc acatctaatg aaaataaaat
      841 tttttcagca ataccttccc cattctgaat ttcattaaag aattgataga atttatattc
      901 cccatctttt tcaagcacca atagatcatt aactaaatat tggttggtct gttctacaat
      961 caataaatcg tctagttcaa ttccccacgc gattaaattt gggtttttca cgtggataaa
     1021 aaaagtgtct cttgggcgtt tgatacaaag tgagttaaga tcgagcttta cagagcggct
     1081 gctattgaaa tcccgataaa gcggaatcgg ctgataagct gccgtttgat ctgaaatttg
     1141 tctaattgaa gccatagtaa aatcctctat ctgttttttt atacagggta ttttactggc
     1201 taaaatacca gtgtcaacca ttattttgga attttttgta aatttaacca gtgttttgac
     1261 cgcttgttat cctttaaaaa gcaataaaaa agcacccata gggtgcttct tatgcttaaa
     1321 agctgttatt ataggctttc agtgaatgta cgagtaataa catcgcgttg ttgctcaggg
     1381 gttaatgagt tgaaacgaac tgcataacct gaaacacgga tcgttaattg aggatatttc
     1441 tcagggtttt gaatcgcatc ttctaatgtt tcacggcgta aaacgtttac gtttaagtgc
     1501 tgaccacctt caactttaat cgttggtgca acattaacgg gtacttcacg gtatgcgata
     1561 tcacctaatt cgctgatagc aacaacttga tcttcttcaa atcctgcttt tgcagctaaa
     1621 caacgagctt catttttagc ttcgtctaat aaccagaaag agtttaataa gttgctgttt
     1681 gatgattctg tgatttgtac acctttaatc attttgatat ctcctctaag attgaaatta
     1741 taaaaaaatt ttaaaaattc aaatttattt gaagttattg tacccctttt tagataaaaa
     1801 gcgataaaaa tttaaccatt tttagacatg attgagttgg ttgggtaaaa aatatacact
     1861 tggtggtttt atgaaaactt ggacagaagc tttaggcgaa gaaaaacaaa aagcgtattt
     1921 tcaacaaatt ttacaatatg tacataacga acgattagcc ggcaaagtca tctatcctcc
     1981 gcaaaacgag gtgtttagtg cttttgcctt aaccgaattt agtgacgtga aagtagtaat
     2041 tttagggcaa gatccttatc acggcccaaa tcaagcccac ggattagcct tttcagttaa
     2101 accgccggtt gccccgccac cttctctggt caatatgtat aaagaattat ctgaagatat
     2161 tgaaggattt caaattccac cgcatggcta tttaattgac tgggcaaaac aaggggtatt
     2221 tatgttaaac accagcttaa cggttgagca aggtagagcc ggttcgcatt cacaaattgg
     2281 ttgggaaatt tttaccgata aagtgatcgc acaattaaat caacaccgtg ataatttagt
     2341 ctttctactt tggggaagcc acgctcagag aaaggggcaa tttatcgatc gtaatcgcca
     2401 ttgtgtgtta gcttctccgc acccatcacc actttccgca tatcgtggat tttttggttg
     2461 taaacatttt tctaaagcca ataactactt gatttctcaa ggaatatccc caattaattg
     2521 gcaattatca gtagacgtta gttattcttc aacacattag tttaaggagc aaacgatgaa
     2581 ttttgattta ccgattgtta gtattgcatt tgttgtgctt gttttagttg cattaagttc
     2641 gactattaaa attgtgccac agggctatca ttggacagta gaacgttttg gtcgttatac
     2701 taaaacgcta agcccggggt tgaacattgt cgtacctttt attgatcgta ttgggcgaaa
     2761 aatgaatatg atggagcaag ttttagatat tccatcgcaa gaggttattt cacgtgataa
     2821 tgcaagtgtt gcgattgatg ctgtatgttt tgttcaaaca gttgatgctc gccgagctgc
     2881 ctatgaagtg aatcatttag agcaagcaat tgttaattta accatgacaa atatgcgtac
     2941 cgtgctcggc tctatggatc tagatgatat gctctctcag cgtgatttaa ttaacggtag
     3001 attactttcc attgtcgatg aggcgactaa tatttgggga gtaaaagtca cccgtattga
     3061 gattcgggat gttcgtccac cgaaagagct ggttgcggca atgaacgctc aaatgaaagc
     3121 tgagcgtaat aaacgtgctg atattcttga agcagaaggg attcgacaag ctgaaattct
     3181 gcgggctgaa ggggaaaaac aatctcgtat tttaaaagca gaaggtgaaa gacaagaagc
     3241 attcttacag gcagaagctc gtgaacgagc cgcagaagcg gaagctaaag ccactcaaat
     3301 ggtttctgaa gcaattgcta aaggcgatac tacagcgatt aactatttta ttgcacaaaa
     3361 atacaccgaa gctttgaaag aaataggttc ttcagataac agtaaagttg tacttatgcc
     3421 gttagaagct ggaaatttga ttggttctgt tgctggtatt gctgaaattt taaaaagcaa
     3481 taaaacaagc tcatagccta aaaccggatt aattgagtat atggagggag tatggactgg
     3541 ttttttaatt ggattggctg gagcatatta ggctttgtac tacttgcatt agaattagta
     3601 ttaccgggtg tctttattat gtggtgggga tttgcctcgc ttattcttgc tttcatcgtt
     3661 gcacttttta cattagggca agcgttacaa attgcgttat ttgcgataat tgcgactatt
     3721 tttagtctta tttggtggaa atatcagcat ggaaaagaca agaaagagga tcagagcagt
     3781 tcccttaact ctcgagagca tgctatgcta ggagctcaag gctttgtggt tgaaatctta
     3841 cctaatggca tcgctcgagg aaaatttggt gatacgactt ggcgtgttat aggtaataat
     3901 ttacaagtag cagataagat tgaagtccag aaagtggagg gaattacctt atttgtccgt
     3961 aagcaataag ttttttctta tttagtcata gaacactaaa caaaaacctt gaacagttgt
     4021 tcaaggtttt ttctctcaag cggttgaaaa tagagtgaaa tttgcaaatt ttcaccctaa
     4081 attaaccgct tgtgtcccac cataataagt tagaaactaa agccaacatt tactttccaa
     4141 ccaaataggt ttgcagattt cactcgactg taatctaaat tcatcgcagt aaaccaattt
     4201 ttataatgca gtttcacccc gacttcgccg tttaattggc gattacgttt cgtcgttgct
     4261 tcactatgca gtgtttttcc tgcaatgtta ctggtaattt tggttatttt tccatttaat
     4321 ctatgcaagt aatttagccc gatataaggg gaaatagagc cttgctcaag ttggaaagtt
     4381 cgctgtaatt tcacgcttgg aacaatattc acatcacgat aacgcattgc agccgtttga
     4441 gtcgcaagtt cgccactttc atttaaacca ttcattttgg tttgaatcca ttgtacgcca
     4501 aggcttggtt caattatcca ttggtttggc ataaattgat aaccgatccg catttcacca
     4561 ccgagcgttg aacctctggt ttctgcagta cctagttgat ggctagcaaa tcgacggctt
     4621 tgttgatatt tctggcgagc aaattgcaat gtagcttcac taaaccaagc gttatctaaa
     4681 tgataacgta agccaatatt taatgctgtt tgttttaagg tagcttgtgc aaatggtttt
     4741 tctaagcggt ttttttgctg tgaaagtgtt gcactaagta ccgttttatc tgccagtttg
     4801 tggtttactc ccaattgtga ttggtggtgt ttaacatcgg tatgcttcga tgaggctgat
     4861 tgtttttgat tcaaatgttc agcccaaaca gtcgtttgca gtggtgtatg gtatgccgta
     4921 aacaaatgtt gctgtgcttg ggttaagcct ttggcatttt ggctacgtag caattcaagg
     4981 ctatctgcat aaatattgtt agatacagca tagtgcagat tatctaattt acgttgttcg
     5041 agtgcagatt gcagccaacc gttataccaa ctacggcgat aggcaatagg tgtagttctt
     5101 aacgcattta ataatgctaa accggcttgc ccgtttgtat cggctttctc atcagttagt
     5161 ggcttattca cttttgcgat aatttcgctc tctttgaaat agagatcttg gattaaccct
     5221 cgtttggcta aattctcgtt ttcgactaat tgtgtaaatc cgccttgata agttgcttta
     5281 tctttcaact gtactacggt cgctgaaaca tcacctgctt cggttttttc tgttaattta
     5341 tcatcaattt ttaacgagcc ggctaaatgt atattggctt gagcattggc aattagccca
     5401 gatttgccca acacttctaa ttggctttgt ccagacgaag aataattttg ctcaacttcg
     5461 gttccattga cagctaaatt tgcatggtta ctgattgttt tcaccttagt tttaccgttt
     5521 aaggccaatc taccttcttc aactgctacc gtatctaaat gattttcgcc gtctaaatgc
     5581 agtgatttgt cccctttttt tgtaatttta aattggctgg cgagtggatt cgaccaatgg
     5641 tcatcacgag ttacggtaat cgtttcatca tttaggaatt gagttgggcc attaacagct
     5701 tttttcaggt taatgagacc ccagccataa acattatcga cccctttttc acctaaatca
     5761 gtggcggtag tcaataaagt atcgcgaatt tgagtcgcag tcagataatc aaagcgttct
     5821 tttaatactg ctaatgaagc agttatagca ggtgctgaaa atgacgtacc ttgctctttt
     5881 gttaagccat attgtggttt tttatgttca tcagcaacgc caattaaaac atgtaaatcg
     5941 cccggtgctg ctacacacca gtttttactt gctccgcagt ggttagagta tgaagcaatt
     6001 ttttgttctt tatctaccgc aacggcagaa attaaatttt tctcaagctc gggaaagtaa
     6061 cgtggtagaa gagccattac gcctggctgt ttcttgctct cattaccagc agcaaagact
     6121 aataaggtgt tgctattttt ggctctcata atagcttgaa gtagcggatt gtgcttattt
     6181 tgcttgatag attgataaaa tttatctaca acattgatgt catcgctaat agaaaaagga
     6241 ttccaactat tattgattgc aaaaacatta ttatctagta atttattcag cccggatgcc
     6301 cattcagcac gttcaattgc catttctttt tctttgcttg ctaataagtc attctttgcc
     6361 atttgatcca gacttggcgt tttttcatcc tcttcgtctt ttgcattttc taattctttc
     6421 ttagcatctt gaataacttt ttctaaagtt cgtttatatg acttcgttgc aacataaagc
     6481 tcagctccct ttgcaatacc accactatag ccatcaccaa gagttttatc tgcttgagcg
     6541 gcaataatac cagctacacc tgcaccgtgt gtttcaagag aaggaataag atcaattcca
     6601 tcttcttttt ctacggggtt tacctcgaat tgagggtaac ttgcatcgta gattcttact
     6661 tcgccttcgg gtgttgttaa ttgatgaata agaggatgaa gatgtgaact atggcgaggg
     6721 tgtttaacca tgaagccctc atccattacg cctagtttaa cacccttgcc actatatttt
     6781 gagctgaggc tctcagataa ttgaatgata ggttgtggat tctctattga ttctgtagcg
     6841 tat
//
LOCUS       DANLRK010000138         6753 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.138, whole genome shotgun sequence.
ACCESSION   DANLRK010000138 DANLRK010000000
VERSION     DANLRK010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6753
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.138"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            76..1053
                     /gene="manX"
                     /locus_tag="PXA33_002010"
     CDS             76..1053
                     /gene="manX"
                     /locus_tag="PXA33_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005612905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HDL5121316.1"
                     /translation="MSIAIIIATHGVAAEQLLKTTEMLIGEQEDVAYIDFVPGENAET
                     IMGKYQALVEGPLSHCDQVLFLVDMWGGSPFNAANRFQDGKSGMDVVAGVNIPMLVNT
                     CMARDDVESLEELVEVALEAGSTGIRSLNHQPEVAEVQTATPAQETAAVQQPEIVDDG
                     RRMTIALARIDDRLIHGQVATVWTKESKVNRIIVVNEGVAKDSVRTTMLKSVAPLGVT
                     AHVVDVPKMIRVYNNPEYADDRVMLLFTNPSDVLALIEGGVPLNSINVGGISHKEGKQ
                     MVSHSVAVDQNDIDAFKAIDAKGVELEVRKVSSDSRQYMMDLLKKHNLI"
     gene            1065..1865
                     /locus_tag="PXA33_002011"
     CDS             1065..1865
                     /locus_tag="PXA33_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005716709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HDL5121317.1"
                     /translation="MELSALQIVLLFLVACISGMGSILDEWQTHRPLIACTMIGLVLG
                     DVTTGVIVGGSLELLALGWMNIGAAVAPDAALASIVSTILVIVGRQDISTGIAIAIPL
                     AAAGQVLTYVVRAITVGFQHAADKSIEDGNLSRLDWLHRGALLLQAMRIAIPALIVAL
                     TAGTDAVQTMLNAIPEVVTTGLKIAGGFIAVVGYAMVINMMRAGHLMPFFFAGFVIAG
                     FTDFNLVALGVLGTVIAMVYIHMHPKYNKSQQVVVAAAPKNDLDNRLD"
     gene            1879..2715
                     /locus_tag="PXA33_002012"
     CDS             1879..2715
                     /locus_tag="PXA33_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005564636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HDL5121318.1"
                     /translation="MTTEIKKVTKSDLNAVVRRSNLFQGSWNFERMQALGFAYAMVPV
                     IKRLYPDPNGPGRKEAIKRHSEFFNTQPFVGAPILGVTIAMEEERANGKEIDNAAING
                     IKVGLMGPLAGVGDPIFWGTVRPVFAALGAGLALKGELLGPILFFALFNIVRLAARYY
                     GVVYGYKKGLDVVQDMSGGLLQKLTEGASILGLFIMGALVQKWTSINVPLVLSTIQKQ
                     DGTIEVTTVQSILDSLMPGLLPLLLTFACMWLLRNRVNAIWIILGFFVIGILGAATGA
                     LA"
     gene            2808..3293
                     /locus_tag="PXA33_002013"
     CDS             2808..3293
                     /locus_tag="PXA33_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HDL5121319.1"
                     /translation="MTNTILILFILLGLVFAIYDQVFMHKLKGTTLLEIRLKKQKTID
                     TWLLIGLIVLSISYGVQNQIQPFTLYLLATCIILSVYLAFFRSPRLLLKQHGFFFANV
                     FFNYDKIYQVNIAEGKADEKILVIDLHSGRRLLAYVENAEDLQPVVDFFGGYKKESEQ
                     K"
     gene            complement(3379..4170)
                     /gene="tpiA"
                     /locus_tag="PXA33_002014"
     CDS             complement(3379..4170)
                     /gene="tpiA"
                     /locus_tag="PXA33_002014"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HDL5121320.1"
                     /translation="MARRPLVMGNWKLNGDKAFTKTLIADLKAELHGVEGCDVAIAPP
                     VMYLAHAEASLAGCGCGCSDKGLIALGSQNVDVNVKGAFTGDISPEMLKEFGAKYIII
                     GHSERRTYHKESDEFIAQKFKVLKDAGLVPVLCIGESEAENEAGKTEEVCARQIDAVI
                     NAHGVEAFNGAVIAYEPIWAIGTGKSATPAQAQAVHKFIRDHIAAKSQAVADQVIIQY
                     GGSVNDANAAELFTQPDIDGALVGGVSLKAPAFAVIVKAAAAAKA"
     gene            4330..5244
                     /locus_tag="PXA33_002015"
     CDS             4330..5244
                     /locus_tag="PXA33_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HDL5121321.1"
                     /translation="MKLNYLYSPPQAVGRLKAEYADFIVREELGYELAGEGEFVAVKI
                     RKTNASTLFVGEKLAKFVGISEREMSYAGLKDRHNRKIRVGSLAGNHFELLLRDVAES
                     EELIYRLQQLQAVGFPNYFTEQRFGRDGHNLTQALRWANGEISVKDRKKRSFYLSAAR
                     SEVFNLVVSQRINDCLTQKILENDYVQLAGSNSFFCVKSDEIAETQARLAQQDLLLTA
                     PLIGERSLELECNPAEKAIIEQHAQLVALMQKERMAAARRAMLCKPQNLEWQFEQEGL
                     RLKFFLDSGSYATALVRELILLTEESEA"
     gene            5415..6203
                     /gene="surE"
                     /locus_tag="PXA33_002016"
     CDS             5415..6203
                     /gene="surE"
                     /locus_tag="PXA33_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HDL5121322.1"
                     /translation="MRILISNDDGFHAVGIQTLAKALRDAGHIVTIIAPDRNRSAASS
                     CLTLVDPLRVHRFDEYNYAVIAGTPADCVHLALNGLFDGKFAEKFDLVVSGINHGANL
                     GDDVVYSGTVAAALEGRHLPLPSIAVSLVGRKHSSFLDGENHFGTAAQVVLDLLEKME
                     TQRFPSNHVLNVNVPNLPYSELKGTMITRLGERSPAAEIVKQKDPRNADVYWIGVTGE
                     PLDESFGTDFYALNRDCVSITPIQVDMTAHKTIATLKGIFNETV"
     gene            6190..>6753
                     /locus_tag="PXA33_002017"
     CDS             6190..>6753
                     /locus_tag="PXA33_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDL5121323.1"
                     /translation="MKLFETIYDKTMQWSKHRFAAFWLSFVSFIEAIFFPIPPDVMLI
                     PMSMAKPQNAFKYAVYTTLASVAGGVIGYFIGLYATDWVQGVIANWGMQANFDKAKLW
                     FETWGVAVVFLAGFSPIPYKVFTICAGVMQMAFFPFVITAAISRFARFILVAKLSAWG
                     GKKYEQKIRRSIELIGWGTIVLAVVAYI"
BASE COUNT         1877 a         1358 c         1491 g         2027 t
ORIGIN      
        1 attgtgtgaa ataattatca tattaaaatc tcgccccagt aaataaacaa cttaaaaaac
       61 cgcaaggagt aacaaatgag cattgctatt atcatagcaa cccatggtgt tgctgcagag
      121 caacttctca aaacaactga gatgttgatt ggtgaacaag aagatgttgc ttacattgac
      181 tttgttcccg gcgaaaatgc agaaacgatt atgggcaaat atcaagcatt ggttgaaggt
      241 ccactatcac actgtgatca agtgctgttt ttggtggata tgtggggagg aagcccattc
      301 aacgccgcaa accgattcca agatggaaaa tcgggtatgg atgttgttgc gggtgtgaat
      361 attcctatgt tagtgaacac ctgtatggct cgtgatgatg tggagtcgtt ggaagaattg
      421 gtggaagtcg cattagaagc cggctcaacc gggattcgtt cacttaatca ccaacctgaa
      481 gtggctgagg tgcaaaccgc tactcctgca caagaaactg cagctgttca acagcctgaa
      541 attgttgatg acggacgtcg tatgaccatt gcccttgccc gtattgatga ccgcttgatt
      601 cacggtcaag ttgcgacggt ttggacaaaa gaaagtaaag tcaatcgcat tattgtggta
      661 aacgaaggtg tagcaaaaga tagtgtacga actactatgc taaaaagtgt tgctcctctt
      721 ggcgtaacgg ctcacgttgt tgatgtacct aaaatgattc gtgtctataa caaccctgaa
      781 tatgccgatg atcgtgtaat gttactcttt accaacccaa gtgatgtctt agccttaatt
      841 gaaggcggtg taccactcaa tagcattaac gtgggcggta tttcgcataa agaagggaaa
      901 caaatggtta gccattctgt ggcggttgat caaaatgata ttgatgcctt caaagcaatt
      961 gatgcgaaag gcgttgaatt agaagtacgc aaagtctcaa gcgatagccg tcagtatatg
     1021 atggatttat tgaaaaaaca taacttaatc taaaggaaat tactatggaa ctttcagcat
     1081 tacaaattgt gcttttattc ctcgttgcct gtatctcagg tatgggctca attcttgatg
     1141 aatggcaaac ccaccgtccg ttaattgcct gtacgatgat aggcttagtg cttggtgatg
     1201 tgactaccgg cgttattgtt ggtggctcat tagagttatt agctttaggt tggatgaaca
     1261 tcggtgccgc tgttgcacca gatgcggcat tagcctctat tgtctcaacc attttagtca
     1321 ttgtaggtcg tcaagatatt tcaactggta tcgccatcgc tattccgctt gcagctgccg
     1381 gtcaagtatt aacctatgtt gtgcgtgcaa tcaccgtagg tttccaacac gcagcagata
     1441 aatcaattga agacggtaac ttaagccgtt tagactggtt acatcgtggt gctttattgc
     1501 ttcaagcaat gcgtattgcc attccggcat taattgtggc attaacagca ggtacagatg
     1561 cggttcaaac aatgttaaat gccattcctg aagtggttac aaccgggctg aaaattgccg
     1621 gtggcttcat cgccgtggtg ggttatgcga tggtgatcaa tatgatgcgt gcaggtcatt
     1681 taatgccgtt cttcttcgca ggctttgtaa ttgcaggctt taccgatttc aatcttgttg
     1741 cccttggggt attaggtacg gttattgcaa tggtttatat ccatatgcac cctaaatata
     1801 acaaaagcca acaggtagtg gtggcagctg caccaaaaaa tgatttagac aaccgtttag
     1861 actaagaggg catttaaaat gacaactgaa atcaaaaaag taacaaaaag cgatttgaat
     1921 gccgttgtac gtcgttcaaa cttattccaa ggttcgtgga actttgagcg tatgcaggct
     1981 cttggttttg cgtatgcgat ggtaccggtt atcaaacgtt tatatccgga tccaaatggc
     2041 ccgggtcgca aagaagcaat taaacgccat tctgagttct ttaacaccca gccattcgtc
     2101 ggtgcaccga ttttaggggt aaccattgct atggaagaag aacgtgccaa cggtaaagaa
     2161 atcgacaatg ccgcaattaa cggtattaaa gttggtttaa tgggaccatt agccggtgtg
     2221 ggtgacccaa ttttctgggg aactgttcgc cctgtatttg ccgcattagg agcaggctta
     2281 gccttaaaag gtgagctttt aggcccaatc ctgtttttcg ccttatttaa catagtgcgt
     2341 ttagccgcac gctattatgg cgtagtgtat ggctataaaa aagggttaga tgttgtacaa
     2401 gatatgagcg gcggtttatt gcaaaaatta accgaaggtg cttcaatctt aggtctgttt
     2461 attatggggg cattggtaca aaaatggacg agcattaacg ttccgcttgt gctttcaacc
     2521 attcaaaaac aagacggtac catcgaagtt accacagtgc aaagcattct cgacagctta
     2581 atgccgggcc tactcccatt attattaacc tttgcttgta tgtggttact tcgtaatcga
     2641 gtaaatgcta tctggattat cctcggtttc tttgttatcg gtatcctcgg cgctgcaacc
     2701 ggtgcattag cttaattgca aaattttctg tgaaaacgac cgcttgaact tgaacattca
     2761 agcggttttt tatacgcttt agcccaaata aattcacttg cttaattatg accaatacca
     2821 tccttattct ctttatttta cttggcttag tattcgccat ttatgaccaa gtttttatgc
     2881 acaaactcaa aggcacaacc ttactggaaa tccgtttaaa aaagcaaaaa acaattgata
     2941 cttggttatt gattggttta atcgtcttgt caattagcta tggcgtacaa aatcaaatcc
     3001 agccttttac actttacctg ctggcgactt gcattattct gtcggtctat ctcgcctttt
     3061 tccgttcccc tcgcttgttg ttaaaacagc acggtttttt ctttgcgaat gtctttttta
     3121 attacgacaa gatttatcaa gtcaatattg cagaaggaaa agcagacgaa aaaattttgg
     3181 taattgattt acatagcgga agaaggctgc ttgcctatgt tgaaaatgcg gaagatcttc
     3241 aaccggtagt ggattttttt ggcggttata aaaaggaaag tgagcagaaa tagtttgcga
     3301 acaaaacaca agcggtcatt tttccctgaa attttgcaaa aaactgagga aaaatgaccg
     3361 cttgtaaaat cggttaaatt aggctttagc agccgctgcc gctttaacga tcaccgcaaa
     3421 agcaggcgct ttaagtgaaa caccgcctac taatgcaccg tcaatatccg gttgagtgaa
     3481 taactctgct gcattggcat cgtttacaga gccaccgtat tgaatgatca cttggtcagc
     3541 cactgcttgc gatttagctg caatatggtc acgaataaat ttatgcactg cttgtgcttg
     3601 tgccggagtc gctgatttgc cggtaccgat tgcccaaatt ggttcgtaag caataactgc
     3661 accgttgaat gcttccacac cgtgcgcgtt gatgaccgca tcaatttgtc ttgcacacac
     3721 ttcttcagtt ttacctgctt cattttcggc ttcagattca ccgatacata atacaggcac
     3781 taagccggca tcttttaata ctttgaattt ttgagcaata aattcatcgc tttctttgtg
     3841 gtaagtacgg cgttctgaat gcccgataat aatatatttt gccccaaact cttttaacat
     3901 ttcaggtgaa atatcaccgg tgaatgcccc ttttacgttc acatcaacgt tttgtgaacc
     3961 aagtgcgatt aagcctttat cactgcaacc acagccacaa ccggcaagtg atgcttcagc
     4021 gtgtgccaaa tacatcaccg gtggagcgat tgctacatca cagccttcaa cgccatgtaa
     4081 ttctgctttt aaatctgcaa ttaaggtttt agtaaacgct ttatcaccgt ttaatttcca
     4141 gttacccatt acaagtggac gacgtgccat tttgtttctc ctatatgaag ttgaacttaa
     4201 atataaacgt tgcaaactat accaaatttt aatgtcgttt tgttgttttt attttgcttt
     4261 ttctgatcta gatcgaaagg tttaacgtat aatgccgaaa tcttattttt tatgttattc
     4321 gagaagtgaa tgaaactgaa ttatttatac tctcctcctc aagctgttgg gcgactaaaa
     4381 gccgaatatg cagactttat tgtgcgtgaa gaactgggct acgaactagc cggcgaaggg
     4441 gaatttgtgg cggttaaaat ccgcaaaacc aacgccagca cgctgtttgt gggggaaaag
     4501 ttagccaaat ttgtcggtat ttccgagcgt gaaatgagtt atgcaggctt aaaggaccgc
     4561 cacaaccgca aaattcgtgt tggttcgctc gccggcaatc attttgaatt attgctacgt
     4621 gatgtagcag aaagcgaaga gttgatttac cgtttgcaac agttgcaagc ggtcggattt
     4681 cccaattatt ttaccgaaca acgtttcggg cgagacggac ataacctgac tcaagccttg
     4741 cgttgggcaa acggggaaat tagcgtgaaa gatcgcaaaa aacgcagttt ctacctttcc
     4801 gctgcccgct cggaagtgtt taatttagtg gtttcacagc ggattaatga ctgcttaacc
     4861 caaaaaatct tggagaatga ttacgttcaa cttgccggct ctaacagctt tttctgcgtg
     4921 aaatcggacg aaattgctga aactcaagcc cgtttagcac agcaagatct acttctcacc
     4981 gctccgctga taggcgaaag atcgcttgaa ttagaatgca atcctgctga aaaagccatc
     5041 atagagcaac acgctcaatt ggttgcctta atgcaaaaag agcgtatggc agccgctcgg
     5101 cgagcaatgc tttgtaagcc acaaaatctg gaatggcaat ttgaacaaga agggctacgg
     5161 ttaaaattct ttttagattc aggcagttat gccactgcac tggtgcgaga gttaattctg
     5221 cttaccgaag agagcgaagc ttagtccata acacataaca agcggtaaga ttttaatcat
     5281 tttttgcaat ttgcaaaact ttaacaaaat cttaccgctt tttgcacttt tagactaaaa
     5341 aaactgtcga aaaattgtta gaattgcgat ctttgtattt agagtctatc aagatgtaag
     5401 atctaaagga aattatgcga attttaatta gtaatgacga tggttttcac gctgtcggca
     5461 ttcaaacttt agccaaagcc ttgcgagatg caggccatat cgtcaccatt attgcaccgg
     5521 atcgtaaccg cagtgcagct tcaagctgcc taaccttagt agatccactt cgggtgcatc
     5581 gttttgatga atataattat gcggtgatcg ccggcacacc tgccgattgc gtgcatttag
     5641 ccttaaacgg cttgtttgac ggcaaatttg cagaaaaatt tgatttggtg gtctccggca
     5701 ttaatcacgg ggcaaattta ggcgatgatg tggtttactc cggtacggta gcggcagcat
     5761 tggaaggccg ccacttgcca ttaccaagta ttgcagtatc actggttggg cgtaaacact
     5821 cttcgttttt agacggtgaa aatcattttg gaacggcagc acaggtagtg ttagatttat
     5881 tggaaaaaat ggaaacgcaa cgttttccat caaaccacgt tctcaatgtt aatgtgccga
     5941 atttacctta ttcagagcta aaaggcacaa tgatcacccg cttaggtgaa cgttcacccg
     6001 ctgcggaaat tgtgaagcaa aaagatcctc gtaatgctga tgtctattgg attggtgtaa
     6061 caggtgaacc actggatgaa agttttggta ctgattttta tgcattaaac cgagattgcg
     6121 tttctatcac accaattcaa gtggatatga ccgctcataa aacgatagca accttaaaag
     6181 gcatttttaa tgaaactgtt tgaaacaatt tatgacaaaa caatgcagtg gtctaagcac
     6241 cgcttcgctg ccttttggtt gagttttgtc agttttatcg aggcgatctt tttcccgatt
     6301 ccgccggatg tgatgttgat tccgatgtcg atggcaaaac cgcaaaatgc gtttaaatat
     6361 gcagtttaca ctacccttgc ttcggttgcc ggtggtgtta ttggttattt tattggctta
     6421 tatgctaccg attgggtgca aggcgtcatc gcaaattggg gaatgcaagc aaactttgat
     6481 aaagcaaaat tgtggtttga aacttggggt gtcgcggtag tatttttagc cggtttttcg
     6541 ccgattccct ataaggtatt taccatctgt gccggtgtaa tgcagatggc gttcttcccg
     6601 tttgtgatta ctgctgcgat ttctcgcttt gcccgtttta ttttggtagc aaaactctct
     6661 gcttggggtg gcaaaaaata tgaacaaaaa attcgccgct caattgaatt aattggttgg
     6721 ggaacaatcg ttttagccgt tgttgcctat ata
//
LOCUS       DANLRK010000139         6706 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.139, whole genome shotgun sequence.
ACCESSION   DANLRK010000139 DANLRK010000000
VERSION     DANLRK010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6706
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.139"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(169..1476)
                     /gene="serS"
                     /locus_tag="PXA33_002018"
     CDS             complement(169..1476)
                     /gene="serS"
                     /locus_tag="PXA33_002018"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HDL5121324.1"
                     /translation="MIDQNLLRTNLEDVATALKIKRNFILDVERVKTLEEQRKTLQVK
                     TETLQAERNTRSKNIGAAKARGEDISALLAEVDSMGNELDTAKVELDKVQAEIRELLL
                     SVPNLPADEVPLGKDDSENLEVLRWGEPRQFDFEVQDHVTLGENLNGLDFAAGVKLTA
                     SRFVVMKGKLARLHRALSQFMLDLHTEQHGYVETNVPFLVNHDTLYGTGQLPKFGEDL
                     FHTQPLTGQDPNEVQRPFGLIPTAEVPVTNLVRDEIIDEDSLPLRYTAHTPCFRAEAG
                     SYGRDTRGLIRMHQFEKVEMVQIVAPEKSMEALEELTGHAEKVLQLLGLPYRKVLLCT
                     GDMGFGAAKTYDLEVWLPAQNTYREISSCSNMWDFQARRMSARCKAKGDKKTRLVHTL
                     NGSGLAVGRTLVAVLENYQNADGSITVPEVLRPYMGGLEVITA"
     gene            complement(1679..2158)
                     /gene="smpB"
                     /locus_tag="PXA33_002019"
     CDS             complement(1679..2158)
                     /gene="smpB"
                     /locus_tag="PXA33_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HDL5121325.1"
                     /translation="MAKKPKVASNTIALNKRARHEYFIEDEIEAGLELQGWEVKALRA
                     GKGNISDSYVIFRHGEAFLFGAMITPLNMASTHIVSDPTRTRKLLLNKRELDSLFGKV
                     NREGYTIVATSMYWKNAWVKVKIGLAKGKKLHDKREDIKDREWQVAKQRIMKNANRG"
     gene            complement(2266..2589)
                     /locus_tag="PXA33_002020"
     CDS             complement(2266..2589)
                     /locus_tag="PXA33_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDL5121326.1"
                     /translation="MNNIDTKIRHTTEAQGNVFEDLGFPIDEAQKLKSASQQLIETKL
                     MLMNEMSNWIDKNNLKQSEAASILGVSRPRISDMVNGKLEKFTLDTLVTFVAKTGKTI
                     QLQLS"
     gene            complement(2582..2932)
                     /locus_tag="PXA33_002021"
     CDS             complement(2582..2932)
                     /locus_tag="PXA33_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL5121327.1"
                     /translation="MNTVISQRKPIHWVGSTLDDIREFPDNIKRELGFDLDLVQQGLL
                     PRDFKAMANLGSGVMEIRVKDPSGAFRLVYLAKFKDAIYCLHAFQKKTQKTAPKDIAV
                     IKARYAAVKELENE"
     gene            complement(2978..4387)
                     /locus_tag="PXA33_002022"
     CDS             complement(2978..4387)
                     /locus_tag="PXA33_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HDL5121328.1"
                     /translation="MFSKIHHKLNNFVQRGLDNHLRLAVTGLSRSGKTAFITSLVDQL
                     LHINKGGNSHLNLFAPARHGQILSVKRVEQGDLTIPRFEYDKNRQSLENQPPQWPEST
                     TGISEIRLAIRYQRQHSLLRHIKETGTLYLDIFDYPGEWLLDLPLLSLSYKEWSQTQQ
                     TIHKDERAELATPWLQAVKKLDLFAKTDENLLAEFSQQYTDYLLACKAQGMQYIQPGR
                     FVLPTENAKGAPVFQFFPLLDLSEAEWEKLEQSDRNSIFHTLKKRYEHYQKKIVKPFY
                     EDYFSQFDRQVILADCLTPLNYGYNEFMEMKLGLQQLFKHFHYGNRSLFHRLFSSNID
                     KLLFIATKADHITSDQVPNLESLMRQLVQEGGRHAEFDGIETGYLAISSIQATDAVKV
                     TQQDKIIKAIRGIRSSDRKQVTLYPGSVPNRIPDKNYWQFNRFEFDQFEPKQLDYSQA
                     IPHFRMDSVLQFLLADMFE"
     gene            4448..5299
                     /locus_tag="PXA33_002023"
     CDS             4448..5299
                     /locus_tag="PXA33_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HDL5121329.1"
                     /translation="MENQKQAEIELKIMLEQQNVALVENYLNQTSPFPVLAHQTELLG
                     NTYYDTPEQFFATQKMGLRVRSVNQQYEITLKTQGEIVGGLHIRPEYNLALPNSQPDF
                     QTLVEKYQLPFENSEQIAESLQAIFSTDFTRKTWLVQVGMSDIEVALDQGIIKNHFGE
                     EPICELEFEIKQGNLGDLFTLVEAMPKADGMWLSNLSKAQRGYLLGQAVKWEQKIATA
                     MDGENSEKLTQLLADFICVGNESLEVLVRFNQCTQQQFSNWQQARAFVKSKDYLLHNL
                     TNLKTMI"
     gene            complement(5336..5812)
                     /locus_tag="PXA33_002024"
     CDS             complement(5336..5812)
                     /locus_tag="PXA33_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121330.1"
                     /translation="MNKLWTVLALTITSHYSFAAAEKIVTKDLPAEGKPAVVLQVFDE
                     SEKIAKPIKGNKLFISKKQTRLCWHSINLPVQNKVTIAEAFYAPAAMKLASPGSKINS
                     SADKKNHTIITEINLANNQVLNRCWGFDKTDPVGKYKMEIQINDHIFKGLEFEIVK"
     gene            complement(5805..5969)
                     /locus_tag="PXA33_002025"
     CDS             complement(5805..5969)
                     /locus_tag="PXA33_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HDL5121331.1"
                     /translation="MDNSLLSLTHEQQQAAVEEIQKLMAQGISAGEAIQIIANQLRDK
                     YQILEKSNNE"
     gene            complement(5987..>6706)
                     /gene="truC"
                     /locus_tag="PXA33_002026"
     CDS             complement(5987..>6706)
                     /gene="truC"
                     /locus_tag="PXA33_002026"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HDL5121332.1"
                     /translation="DILYQDNSLIAINKPAGVLVHRSWLDKHETVFAMQTLRDQIGQH
                     VFPIHRLDRPTSGVLLFALNSETARIMSEQFEQHLVQKTYLAVVRGYLQGSAQIDYPL
                     KVILDKIADKFSQEKEAQAAITDYKGLATVEMPYPVGKHQTSRYSLVELSPKTGRKHQ
                     LRRHLKHLFHPIMGDSKYGDLHQNRALVEKSGVSRLMLHAHKLQFQHPKNLQKIEIIA
                     SLDEQWQRLFAFFDWNFTQYY"
BASE COUNT         1859 a         1295 c         1413 g         2139 t
ORIGIN      
        1 ccattattgc atttcgttta gggagaatga aaaatcctat tcgtttagat acagtgcaaa
       61 accgccgagt agttactgaa atttttatga attatcctga aaaagattag ggataagatt
      121 tattaaaaaa accaacccca tttctgagat tggtttcatt tattaatctt aagcggtaat
      181 tacttccaaa ccacccatat aaggtcttaa cacctcaggc acagtaatag agccatcggc
      241 attttggtag ttttctaaca cagcgaccag tgtgcgacca accgctaaac ctgagccgtt
      301 taaggtgtga accaagcgag ttttcttgtc gcctttagct ttacaacgtg cagacattcg
      361 gcgggcttgg aaatcccaca tatttgagca agaagaaatc tcacggtaag tgttttgtgc
      421 cggtagccaa acttctaagt cgtaggtttt tgctgcacca aagcccatat caccagtgca
      481 gagtagcact ttgcggtatg gcaagcctaa aagctgtaag actttttctg cgtgaccggt
      541 taattcttct aaggcttcca ttgatttttc aggggcgaca atttgcacca tttccacttt
      601 ttcaaattgg tgcatacgaa ttaaaccacg agtatcacgc ccataagaac cggcttctgc
      661 acggaaacaa ggggtgtggg cggtataacg cagtggtaag ctgtcttcat caatgatttc
      721 atctcgcact aagttcgtta ctggcacttc agcggttgga atcaaaccga atgggcgttg
      781 cacctcgttt ggatcttgcc ctgttaatgg ttgagtatgg aataaatctt cgccaaattt
      841 tggtagctga cctgtgccgt atagagtgtc gtgattgact aaaaatggca cattggtttc
      901 cacatagccg tgttgttcag tgtgtaaatc caacataaat tgagataatg cacggtgtaa
      961 gcgggcaagt ttacctttca ttaccacaaa acggctggcg gttaatttta caccggcagc
     1021 aaaatctaaa ccgtttaagt tttcgccaag ggtaacgtga tcttgaactt caaagtcgaa
     1081 ttgacgtggt tcgccccaac gtaatacttc taagttttcg ctatcgtctt taccgagtgg
     1141 tacttcatct gccggtaagt ttggcacaga aagcaacaac tcacggattt ctgcctgtac
     1201 tttatccaat tctacttttg ccgtgtctaa ttcgttgccc attgaatcta cttcggctaa
     1261 taaagctgaa atatcttcgc cacgagcttt tgccgcaccg atgtttttgg agcgagtgtt
     1321 gcgttctgct tgtaaggttt cggttttaac ttgtagcgtt ttacgctgtt cttcaagggt
     1381 ttttactcgc tctacatcta aaataaaatt acgtttgatt ttaagagctg tagcaacgtc
     1441 ttctaaattg gtacgaagta ggttttggtc gatcattgta tgttccttag tttaaatttg
     1501 tgtttcactc tctcccttta tgggagagag accgaaaaat atttcgttca gaaatatttt
     1561 tcagagagag ggcaagcgtt gtttttcctt gaacttttac aaatccccct ttccctgaaa
     1621 aattgctgaa cgcaattttt ctgtccctct cccacaaggg gagagggtaa ttattcattt
     1681 agccacgatt ggcgtttttc ataatacgct gtttagccac ttgccattca cggtctttaa
     1741 tatcttcacg tttatcgtgc agttttttcc ctttggctaa gccgattttt actttcaccc
     1801 acgcattttt ccaatacatt gaggtggcaa caatggtata accttcacga ttaactttac
     1861 cgaacagtga gtctaactca cgtttattca gtagaagttt gcgggtgcgg gtcgggtcgc
     1921 tgacaatatg ggttgatgcc atattcaaag gcgtaatcat tgccccgaat aagaaggctt
     1981 cgccgtggcg aaagatgaca tagctatcgc tgatattgcc ttttccggca cgcaatgctt
     2041 tgacttccca gccctgtagc tctaagccgg cttcaatttc atcttcaatg aaatattcgt
     2101 gtctggcacg tttatttaac gcaatcgtat ttgatgcaac tttaggtttt tttgccataa
     2161 tttgtgttta ttaataaaaa attagtcaca attttacttg aaataacctt gttagtgaat
     2221 gataaaagca acaagcggtt gaatttgagt aaaattttgc aaatatcagg ataattgcaa
     2281 ttggattgtt ttgccggttt ttgcaacaaa agtgactaag gtatctaggg taaacttttc
     2341 taacttgcca ttgaccatat cagaaattcg agggcggcta acacctagaa tagaagccgc
     2401 ttctgattgt ttgagattat ttttatcaat ccagttgctc atttcattca ttagcatcag
     2461 tttagtttca atcagttgct gactggcaga tttcaatttt tgagcttcat caatagggaa
     2521 accaagatct tcaaaaacat taccctgagc ttctgtagta tggcgaattt tagtatcaat
     2581 attattcatt ttctaactcc tttacggcag catagcgtgc tttaatgaca gcaatgtctt
     2641 taggggctgt tttttgcgtt ttcttttgga aagcgtgtaa acagtaaatt gcatctttaa
     2701 atttagctaa atacactaag cgaaaagccc ctgagggatc ttttactcga atttccatta
     2761 cgcctgaacc caaatttgcc atcgctttaa aatccctcgg taataagcct tgctgaacta
     2821 aatcaagatc aaaaccgagt tctcttttaa tgttatccgg aaattcacga atatcatcta
     2881 aggttgatcc aacccagtga attggttttc gttgggaaat tacagtattc ataatgtata
     2941 aaattttata cctttaatgg aaatatgcaa caagatttta ctcaaacata tccgccaata
     3001 aaaattgtag caccgaatcc attctgaagt gtggaatcgc ttggctataa tcaagttgct
     3061 tgggctcgaa ttggtcgaat tcaaaacggt taaattgcca gtaattttta tcgggtatgc
     3121 gatttggcac gctgccaggg tagagtgtca cttgttttct atcgctggaa cggatccctc
     3181 ttatcgcttt aatgatttta tcttgctgag tcactttcac cgcatcggtt gcttgaatcg
     3241 aggaaatggc taaatagccg gtttcgatac catcaaattc tgcgtgtctt cccccttctt
     3301 gcactaattg tcgcattagg ctttccaaat tcggcacttg gtcgctagta atgtgatctg
     3361 cctttgttgc gataaatagc aatttatcaa tgttggacga aaacagacgg tgaaatagcg
     3421 aacgattgcc ataatggaaa tgtttaaaca gttgctgtaa ccctaatttc atttccataa
     3481 attcgttata gccataattt aacggtgtga ggcagtctgc caaaatcact tggcggtcaa
     3541 attgagagaa gtaatcttca taaaacggtt tgacgatttt tttctggtaa tgctcatagc
     3601 gttttttgag ggtatggaaa atactgtttc tgtcgctttg ttccaacttt tcccactcag
     3661 cttcgcttaa atctaataac ggaaaaaatt ggaataccgg tgcacctttg gcattttcag
     3721 ttggcaacac aaatcgcccc ggctgaatat attgcattcc ttgagctttg caggcgagca
     3781 aataatcggt gtattgttgg ctgaattctg ccagtaaatt ttcatcggtt tttgcaaaaa
     3841 gatcgagttt tttaaccgct tgtaaccaag gtgtggcaag ctctgcacgc tcatctttat
     3901 gtatggtttg ttgcgtttgc gaccattctt tataggaaag ggagagcagg ggtaaatcta
     3961 acagccattc gcccgggtaa tcgaaaatat ccaaataaag cgtgccggtt tctttaatat
     4021 gacgtagcag agaatgttgc cgctggtagc gaatcgcaag gcgaatttcg ctaatgccag
     4081 tggtggattc aggccattga ggtggttgat tttcaaggct ttggcggttt ttatcatatt
     4141 caaagcgggg aatagttaaa tccccttgct caacgcgttt taccgataga atttgcccgt
     4201 ggcgtgcagg cgcaaacagg tttaaatgcg aattaccgcc tttgttgata tgcaaaagct
     4261 gatcaaccaa gctggtaata aatgccgttt tgccactacg gcttaagccg gtaacggcga
     4321 ggcgtaaatg gttgtcgagt ccacgctgga caaagttatt cagtttatgg tgaatttttg
     4381 aaaacatttt gcaaaattta ttgataaaat aaccgcttga ttatacaacg ttgcaggggc
     4441 gtgtgaaatg gaaaaccaaa aacaagcaga aatcgaactg aaaataatgt tggaacagca
     4501 gaatgtggcg ttagtggaaa actacttgaa tcaaacctcg ccattccctg tgcttgctca
     4561 tcaaacggaa ttgttaggca acacctatta tgatacgccc gagcaatttt ttgccacgca
     4621 aaaaatgggg ctgcgggtgc gttctgtgaa tcaacaatat gaaatcacgt taaaaacaca
     4681 aggggaaatt gtaggcggtt tgcatattcg tccggaatat aatttagctt tacccaatag
     4741 ccagccggat tttcaaacat tagtggaaaa ataccagttg ccgtttgaaa attcagagca
     4801 gattgcagaa agcttgcaag cgatattcag caccgatttc actcgtaaaa cttggttggt
     4861 gcaagtgggg atgtctgaca ttgaggtagc gttagatcag ggcatcatta aaaatcattt
     4921 tggtgaagag ccgatttgtg aattggagtt tgaaatcaag caaggaaatc taggcgactt
     4981 atttactttg gttgaggcaa tgccaaaagc ggatgggatg tggttgagta atttaagcaa
     5041 agcccagcgg ggttatttgc tcggacaagc ggttaaatgg gagcagaaaa ttgcaactgc
     5101 aatggatggt gaaaacagtg aaaaattaac ccaattatta gctgatttta tttgtgtcgg
     5161 caatgaaagt ttggaagtgc tggtacgttt taatcaatgc actcagcaac aatttagcaa
     5221 ctggcaacag gcgagagcct ttgtgaagag taaagattat ttgctgcaca atttaaccaa
     5281 tttaaaaaca atgatttagc aaaaagccac agggtgttac tctgtggctt ttaggttatt
     5341 ttacaatttc aaattctaaa cctttgaaaa tgtgatcgtt aatttgaatt tccattttgt
     5401 atttaccgac agggtcagtt ttatcaaaac cccagcaacg atttaatact tgattatttg
     5461 caaggtttat ttcagtaatg atagtatgat ttttcttgtc tgcagaagaa ttaattttag
     5521 agccgggaga agctaatttc attgcagcag gggcatagaa agcttctgca atagtaactt
     5581 tattttgtac aggaagatta atactgtgcc agcaaagcct tgtttgtttt ttgctaataa
     5641 agagcttatt tcccttaata ggttttgcaa ttttttcact ttcatcaaaa acctgtagca
     5701 cgactgcagg ttttccttct gccggtaaat ctttagtgac tattttttca gcggcagcaa
     5761 agctatagtg ggaagttatt gttagggcaa gcactgtcca taatttattc attgtttgat
     5821 ttctccaata tttgatattt gtcacgtaat tgattagcaa taatttgaat tgcctctccg
     5881 gcactgatgc cttgtgccat cagtttttga atttcttcta ctgcggcttg ttgttgctcg
     5941 tgtgtcaatg ataaaagtga attatccatt caatcctctt ttttgtctaa taatattgag
     6001 taaagttcca gtcaaaaaat gcaaagaggc gttgccattg ctcatcaagc gatgcaatta
     6061 tctctatttt ttgcaaattt ttcgggtgct gaaattgcaa tttatgggcg tgtaacatta
     6121 aacgacttac gcctgatttt tcaactaaag cacggttttg gtgtaagtcg ccatatttgc
     6181 tgtctcccat aatcgggtgg aacaggtgtt tcaggtggcg gcgaagctga tgtttccgcc
     6241 cggttttagg gctaagttca accaaactat aacgagaggt ttggtgcttg ccgacagggt
     6301 aaggcatttc aaccgttgct aagcctttat aatcagtaat cgccgcttga gcctcttttt
     6361 cctgagaaaa tttatccgca attttatcta aaatcacttt gagcggataa tcaatttgtg
     6421 ccgaaccttg taaatagcca cgcaccaccg ccaaataagt tttttgtact aggtgttgtt
     6481 caaactgctc gctcattatt cgggcggttt cgctgttgag agcaaacagc aatacacctg
     6541 aggttgggcg gtcaaggcgg tgaatcggaa aaacgtgttg cccgatttga tccctcaggg
     6601 tttgcattgc aaacaccgtt tcgtgtttat ctaaccaaga acgatgcacc aacactccgg
     6661 caggtttgtt gatggcgata agcgagttgt cttggtataa aatatc
//
LOCUS       DANLRK010000140         6704 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.140, whole genome shotgun sequence.
ACCESSION   DANLRK010000140 DANLRK010000000
VERSION     DANLRK010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6704
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.140"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(153..908)
                     /locus_tag="PXA33_002027"
     CDS             complement(153..908)
                     /locus_tag="PXA33_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005613463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HDL5121333.1"
                     /translation="MKLKSFKPMFMLLSVAFLTACSSSINPETGVRDDPLEGFNRAMW
                     KVNYDYLDPYVLKPVAKGWKEYVPSPVKTGLINVANNLDEPVSFVNRLIEGEGKKAMV
                     HFNRFWINTVFGLGGLIDWASLDEGLRLNNGQRGLGESLGAHGVPSGSYIMMPAYGAT
                     TPRQGIGSAVETGYSVLTYVGSPWSIAKFVVQGVDTRSKLLEQDALLEQAQDPYVTFR
                     EAYFQNLEFKVKDGNVEQSQQEQLSEEELKNID"
     gene            1063..1380
                     /gene="metJ"
                     /locus_tag="PXA33_002028"
     CDS             1063..1380
                     /gene="metJ"
                     /locus_tag="PXA33_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011608626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HDL5121334.1"
                     /translation="MADWNGEYISPYAEHGKKSEQVKKVTVSIPIKVLEILTNERTRR
                     QIKNLRHATNSELLCEAFLHAFTGQPLPNDEDLMKDRADEIPEAAKQKMCELGIDPDE
                     WQY"
     gene            1428..1658
                     /locus_tag="PXA33_002029"
     CDS             1428..1658
                     /locus_tag="PXA33_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF997 family protein"
                     /protein_id="HDL5121335.1"
                     /translation="MYQQLTTEARWAVLLTLIYLLGWVGFAYFSPQGRGIFGFPIWFE
                     LSCIFLPVLFSLVATIVVKKVYKNIDLEVNNE"
     gene            1651..3075
                     /gene="panF"
                     /locus_tag="PXA33_002030"
     CDS             1651..3075
                     /gene="panF"
                     /locus_tag="PXA33_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HDL5121336.1"
                     /translation="MNSEMILPLIAYLIFVFGVAFYAYRKRQGGSFISEYYIGGRSMS
                     GFVLAMTTAATYVGASSFIGGPGAAYKYGLGWVLLAMIQVPAVLLSLGALGKKFALLA
                     RKHNSVTINDMLLARYQNKFVVWIAGLALLLSFFAMMTVQFIGAGRLLETTLGIPYQT
                     AVMIFAITVGLYTFIGGFRAVVLTDTIQGLVMIVGTSILLAGVIYATGGVENAMHTLE
                     AINPQLTTPYGIDERPLDFTFMTSFWVLVCFGLIGLPHIAVRSMACKNSQALHRAMII
                     GTVVIAILMFGMHLSGVLGRAVVPDLKVPDQVIPTLMVQVLPPVVAGIFLAAPMAAIM
                     SSIDSMLIQSSSTLIKDLYLAIKPNAINNEKKIKLFSTITTLTFTVLLVFAALTPPEM
                     LIWLNLLSLGGLEATFLWVIVLGLYWEKANATGAIYSMLAGLSCYVLMTTFKIAIFSF
                     HAIVPSLLIGLIAFLIGNRFAPKV"
     gene            complement(3152..4342)
                     /gene="nhaA"
                     /locus_tag="PXA33_002031"
     CDS             complement(3152..4342)
                     /gene="nhaA"
                     /locus_tag="PXA33_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HDL5121337.1"
                     /translation="MEKLKEFLKSEASGGVLLLVFAFAAIVCANTSLSDFYFEFLQTI
                     VSVQFGEFALNKPLLLWVNDGLMAVFFTLVGLEVKKELFEGSLSSYKKAIFPAIAAVG
                     GMIVPAIIYWAINQNHPEYHNGWAIPMATDIAFAVGIVALLGTRVPLALKAFLLALAI
                     IDDLGAIVVIAIFYSQEISVQALTIAAIAIIGLVLLNRFKVTSLCAYLVVGLVLWTSV
                     LKSGVHATLAGVIIGFCVPLKGKHGERPAEELEHILAPWCAYMILPIFAFANAGVYLL
                     EMGMEQLSSPLPLGIALGLIIGKTLGVFGFSYLAVLFRLASLPQGVNFKQIFAIAVLC
                     GIGFTMSMFLASLAFDVSEAGESITALARLGILLGSAVSAILGYTLLRMATKQNNPEN
                     NATY"
     gene            4510..4938
                     /locus_tag="PXA33_002032"
     CDS             4510..4938
                     /locus_tag="PXA33_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDL5121338.1"
                     /translation="MTHDWLYWALASALFAALTAIFAKVGLQGVDSDFATFIRTVVII
                     IALIAFLTYSGKWQPLDSLSSKNWLFLILSGLATGLSWSAYFKALQMGNASQVAPIDK
                     FSLVLVTLFAVIFLGERPSGQDWLGIGLVAGGVLLLAFKR"
     gene            4944..5666
                     /locus_tag="PXA33_002033"
     CDS             4944..5666
                     /locus_tag="PXA33_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HDL5121339.1"
                     /translation="MQNFANIEIAFIHRKAPLPSDFSYPEMPGGLTEKQVQKWQSRRV
                     AFFLLTELFKKHHLDLDLLADMQRSPSGRTFVDSEQIDFNISHSGEWIAVIFSYSFAK
                     LAVGIDIEHPQKIRRYHDLICHYGNAEEQNVLLANHSPILSNLAERFYLSWCLREAIL
                     KSQGVGIVKLSEVKHLPLEKQIFSAHCPKGKLHFVSELPFYLSYFYQQPQNMLLSEPL
                     LYHWQNGQFQPIKCQSLVYDVN"
     gene            5676..6095
                     /gene="psiE"
                     /locus_tag="PXA33_002034"
     CDS             5676..6095
                     /gene="psiE"
                     /locus_tag="PXA33_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HDL5121340.1"
                     /translation="MPKKSLEEHHSSFGKIASETFQWIMNIALLVTGALLSYSLFAEA
                     YSLYELSFSDSEKFQIVEKIVIFFLYFEFLALIVKYFKSNYHFPLRYFLYIGITAMVR
                     LIIVDHSNALHTLLFALSILVMIIALYIVHTDRLHKS"
     gene            6104..>6704
                     /locus_tag="PXA33_002035"
     CDS             6104..>6704
                     /locus_tag="PXA33_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HDL5121341.1"
                     /translation="MKLTQYQHNDLQILNRETVYKGHFELQKITFRHKLFSGEMSGEV
                     VRELLIKGEAAALIAYDPTLDKVVLIEQVRIGAYDAGQKKSPWLLELVAGMVDKGNEE
                     PAEVAMREAKEEAGLEVTTVQHALSVWDSPGGQLERLHLFLGLIDSTQVKSGEVHGLL
                     EEENEDILVHVVSREQAYQWVEDGTIDNVIAVIGLQWLQL"
BASE COUNT         1936 a         1451 c         1361 g         1956 t
ORIGIN      
        1 aatatttctg aacgaaatat ttttcggtct caatactcgc tacgctcgct cctgtggagc
       61 cgttggcaaa gccaacgttc aaatgtaaac atttgtccca caaggggaga ggtgcgcttt
      121 agatgaggcg taccatccct cgttcacatc aattaatcaa tattcttcaa ctcttcttcc
      181 gaaagctgct cttgttggct ttgctcaaca ttgccatctt tcactttaaa ttcaaggttt
      241 tggaaataag cttcacggaa ggtaacataa gggtcttgag cttgctctaa caacgcatct
      301 tgttctaata atttagaacg ggtatctacc ccttgtacca caaatttagc gatagaccaa
      361 ggtgacccaa cataagtcag caccgaataa ccggtttcca ccgcactacc aataccttga
      421 cgaggtgtgg ttgctccata agccggcatc ataatatagg agccggaagg cacgccgtga
      481 gcgcctaaac tctcacctaa accacgttgt ccgttattta aacgtaaccc ctcatctaaa
      541 ctcgcccagt caattaaccc acctaaacca aacacagtgt tgatccagaa gcggttgaag
      601 tgaaccatcg cttttttacc ttctccttca attaagcggt ttacaaagct caccggctca
      661 tcaaggttat tagcaacatt aattaagcct gttttaaccg ggcttggtac atactctttc
      721 caacctttag ctaccggttt taacacataa gggtctaaat agtcgtaatt gactttccac
      781 atcgcacggt taaacccctc aagtgggtcg tctcgcacgc cggtttctgg gttaatgctt
      841 gatgaacaag cggttaaaaa tgccacactg agtagcataa acatcggttt gaacgacttg
      901 agtttcataa attgaacctt ttgcagagaa atttgtccat tgtagcgaag ttgataaaaa
      961 atacaaaaca aaaagccgtt ttgcctctag aaatctagac gtctagattg agatatagtt
     1021 cgcttttgat tacaatacag gcaacattta aggagcaaac aaatggcaga ctggaacggc
     1081 gaatatatta gcccttatgc cgaacacggc aaaaaaagtg aacaagtcaa aaaagtgacg
     1141 gtatccattc caattaaagt attggaaatt ttaactaatg agcgaacccg tcgccaaatc
     1201 aaaaatcttc gtcacgcaac caacagcgaa ttattgtgcg aggctttttt gcacgccttt
     1261 accggtcagc ctttgccgaa tgatgaagac ttgatgaaag acagggcaga tgaaatccct
     1321 gaagcggcaa aacaaaaaat gtgtgagctg ggaatagacc cggacgaatg gcaatattag
     1381 acacaccttg caactgcgag gttcttttta tgttttaagg agactttatg tatcaacaat
     1441 taacaacaga agcacgttgg gctgtgttgc ttacgctgat ttatctgctc ggctgggtag
     1501 gttttgctta cttttcaccg caaggcagag gcattttcgg ctttccgatt tggtttgaac
     1561 tttcttgcat tttcttaccc gtactattta gcttagttgc caccattgtc gtcaaaaaag
     1621 tgtataaaaa tatcgatctt gaggtgaata atgaatagtg aaatgatttt acccctgatt
     1681 gcttatttaa tttttgtgtt tggcgtagcg ttttatgctt accgtaaacg ccaaggcgga
     1741 agctttattt cggaatacta tatcggtggg cgttcaatgt cgggctttgt gttagcaatg
     1801 acaaccgccg ccacttatgt gggggcaagt tcctttatcg gtgggccggg tgctgcctat
     1861 aaatacggtt taggctgggt gttactggca atgattcaag tgcccgcagt gctactctct
     1921 cttggagcat taggcaaaaa atttgcctta cttgcccgca agcataatag tgtcaccatt
     1981 aacgatatgt tattagcccg ttaccaaaac aaatttgtgg tttggattgc aggactagcc
     2041 ttattacttt cgttttttgc gatgatgacg gtgcaattta tcggtgccgg tcgcttactt
     2101 gaaaccacac tcggaatccc ttaccaaaca gcggtaatga tttttgccat cacagtcggg
     2161 ctttatacct ttatcggcgg ttttagagcg gtggtgctaa ccgatactat tcaaggttta
     2221 gtaatgatag tcggcacaag cattttactc gctggtgtga tttatgccac aggcggagtg
     2281 gaaaatgcaa tgcacaccct tgaagccatt aacccacaac ttaccacccc ttacggcatt
     2341 gacgaacgcc cgcttgattt cacctttatg acttcatttt gggtattggt ttgctttggt
     2401 ttaatcggct taccgcatat tgcggtcaga agtatggcat gcaaaaacag ccaagccttg
     2461 caccgagcaa tgattatcgg cacggtagtc attgcaattc taatgtttgg aatgcacctt
     2521 tcaggcgtat taggcagagc ggttgtgcct gatctcaaag tgccggatca agtcattcca
     2581 acactaatgg tacaagtgct accaccggtg gttgccggta ttttccttgc cgccccaatg
     2641 gcagcgatta tgtcttcgat tgactcaatg ttgattcagt cgtcttcaac cttaattaaa
     2701 gatctctatc tggcaattaa gccgaatgcc attaataacg agaaaaaaat caaattgttc
     2761 tccactatta ctactcttac ttttacggta ttactcgtgt ttgccgccct aaccccgcct
     2821 gagatgttaa tttggctgaa cctactctct ttaggtggtt tagaagcgac tttcctatgg
     2881 gtaattgtac tagggttgta ttgggaaaag gcaaatgcca ccggtgctat ttactcaatg
     2941 ctggcaggct taagttgtta tgtattaatg actaccttta agattgctat ttttagcttc
     3001 cacgccattg tgccttcact gctaattggt ttgattgctt tcttaatcgg caaccgattt
     3061 gctcctaagg tgtaaaataa aataggcata gtggtatgga agttcactat gcctattttg
     3121 caaaatttcc gctcaattca accgcttgta gttaataagt ggcattattt tctggattat
     3181 tctgcttagt cgccattcta agtaacgtat aacctaaaat cgctgaaacc gcagagccga
     3241 gtaaaatacc taatcgagct agagcggtaa tactttcccc agcctcactt acatcaaagg
     3301 caagacttgc taagaacatc gacattgtaa aaccgatacc gcatagcacc gcaatggcaa
     3361 aaatttgctt aaaattaacc ccttgcggca ggctggcaag gcggaataat actgctaaat
     3421 aactaaaacc gaatacacct aaggttttgc cgataattaa gccgagcgca atacctaacg
     3481 gcaatggtga tgagagttgc tccattccca tttctaataa gtaaacgccg gcattagcga
     3541 aggcgaagat cggtaaaatc atataagcac accaaggggc aagaatatgt tctagctcct
     3601 ctgccggtcg ttccccatgt ttacctttca gcggtacgca gaagccgatg atcacgccgg
     3661 caagggtagc gtgaacccct gattttaaaa cggaagtcca tagcactaag cctactacca
     3721 aataagcaca gagtgaggtc actttaaagc ggttaagtag cactaagccg ataatcgcaa
     3781 tcgcggcaat agttaaggct tgcacactga tttcttgcga atagaaaatg gcgattacca
     3841 caatcgctcc tagatcatca ataatcgcta atgccaacaa aaatgccttt aaagccaatg
     3901 gcacacgagt gcctaataac gccacaatgc ccaccgcaaa ggcaatatca gttgccattg
     3961 gaattgccca accattatga tattcagggt ggttttgatt aatcgcccaa taaataatcg
     4021 ctggaacaat cataccacct actgctgcta tcgcaggaaa aatagctttc ttataacttg
     4081 aaagcgaacc ttcaaacaac tcttttttta cttccagccc caccaacgta aaaaacactg
     4141 ccattaagcc atcgttaacc cagagtaata gcggtttatt caaggcaaat tcaccaaact
     4201 gtacactaac aatcgtttgt aaaaactcaa aatagaaatc acttaaggag gtgttggcgc
     4261 acacaattgc tgcaaaagca aaaaccagaa gcaatacgcc tcctgaggct tcagatttta
     4321 aaaattcttt taatttttcc acttaacttt ctcctattaa tatttgttta ttgtattttc
     4381 atttctatac attccatctt ttaccaaaaa ttttgcgaag atcaaataga aaatttgata
     4441 aactagaact aactcgcatt ttattgctcc atatgattag aaattaacct aaaatattaa
     4501 aattttgtta tgacacacga ttggctctat tgggcattag cctctgcact ttttgcagca
     4561 ttaaccgcca tttttgccaa agttggctta caaggcgtag attctgattt cgccactttt
     4621 attcgcacgg tagttattat tattgcactt attgcctttc tcacttacag cggaaaatgg
     4681 cagccgttag atagcttgag cagcaaaaac tggctgtttt taatcttatc cggcttggca
     4741 acaggattgt cttggtcggc gtatttcaaa gcattgcaaa tgggtaatgc ttcccaagtc
     4801 gctccaatcg ataaattcag cttggtgtta gttacccttt ttgcagtaat tttcttaggc
     4861 gaacgcccaa gcggacaaga ttggcttggc attggcttag ttgctggtgg cgtactgtta
     4921 cttgccttca agcggtgatt tttatgcaaa attttgcaaa tattgaaatt gcgtttattc
     4981 atcggaaagc acctctgccg agtgatttct cctaccctga aatgcccggc ggtttaactg
     5041 aaaaacaggt gcaaaaatgg caaagccgcc gagtcgcctt ttttctgctg acggaattgt
     5101 ttaaaaaaca ccatctggat ttagatttat tagctgatat gcaacgctcc ccaagcggtc
     5161 gcacttttgt cgattctgag caaattgact ttaacatcag ccattcaggg gaatggatag
     5221 cggtcatttt ttcgtattct tttgcaaaat tagcggtcgg cattgatatt gaacacccac
     5281 aaaaaatcag acgttaccac gatttaatct gccattacgg aaacgctgaa gaacaaaatg
     5341 ttttattagc caaccactcc cccattctaa gcaacctcgc cgaacgcttt tatttgagct
     5401 ggtgcttgcg agaggcgata ttgaaatcgc aaggggtggg gattgtgaaa ctgtccgaag
     5461 taaaacattt accgttagaa aaacagatct tttccgccca ttgtccgaaa ggcaagctgc
     5521 attttgtgag cgaactgccg ttctatttaa gctacttcta tcaacaacct caaaatatgc
     5581 tattatctga acctttgcta taccattggc aaaacgggca gtttcagcct ataaagtgcc
     5641 aatcacttgt ttatgatgtt aattaaggat aacgaatgcc taaaaaatct ttagaagaac
     5701 accattccag ttttggcaaa attgcttccg aaacctttca gtggattatg aatattgcct
     5761 tgctagtaac aggagcattg ctttcttact cgctgtttgc tgaagcctat tcactctatg
     5821 aactttcctt tagcgacagt gagaaattcc aaattgtaga aaaaatagta attttctttc
     5881 tctattttga gtttcttgcg ttgattgtga aatatttcaa atcgaattat cactttccgt
     5941 tacgctattt cctctatatc gggattactg ccatggtgcg tttgattatt gtcgatcatt
     6001 ctaatgcgtt gcatacacta ttattcgcac tctctatttt ggtgatgatt atagcattat
     6061 atattgtaca caccgatcgt ttacataaaa gctaggaact aatatgaagc taacccaata
     6121 tcagcacaat gatttacaga ttctaaaccg agaaaccgtc tataaagggc attttgagct
     6181 acaaaaaatc actttccgcc ataagctgtt ttccggtgaa atgagcggtg aagtggtgcg
     6241 tgaactctta attaaaggcg aagcggcggc tttaattgcc tatgatccaa ccttagataa
     6301 ggtagtatta attgagcaag tgcggattgg agcttacgat gcaggacaga aaaaatcccc
     6361 ttggttgctg gaattggttg ccggtatggt agataaaggc aacgaagagc cggcagaagt
     6421 ggcaatgcgt gaagccaaag aagaagccgg cttggaagtc acaacagtgc aacacgcctt
     6481 aagcgtatgg gacagtcccg gtggacaact ggaacgcttg cacctgtttc tcggcttgat
     6541 tgacagcacc caagtgaaat ccggcgaggt acacggctta ctggaagaag aaaatgaaga
     6601 cattttggtt cacgttgtaa gccgtgaaca ggcatatcaa tgggtagaag acggcacaat
     6661 tgacaatgtg attgcggtaa ttgggctaca atggttgcaa ttaa
//
LOCUS       DANLRK010000141         6697 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.141, whole genome shotgun sequence.
ACCESSION   DANLRK010000141 DANLRK010000000
VERSION     DANLRK010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6697
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.141"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(243..854)
                     /locus_tag="PXA33_002036"
     CDS             complement(243..854)
                     /locus_tag="PXA33_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDL5121342.1"
                     /translation="MQLTFNDFQNYYNGKGDLTMFSFYGKKTWENGIYVGIDGSIGRA
                     NNKLSHQSQTIKLARNVYSLGLTVGKTLEVASFNLQPSFGMRYHHLTSANAQLENTKF
                     ETDRVDLLAIQAGLALNKTFIFNELKVKPELGSYFVDASHGTLKTKLNEFSLNQNIGR
                     YFKQEVGITLHYKGVSSSVHAGFTKGNTLQEQKFISLKVGYEW"
     gene            complement(903..1718)
                     /gene="cysE"
                     /locus_tag="PXA33_002037"
     CDS             complement(903..1718)
                     /gene="cysE"
                     /locus_tag="PXA33_002037"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HDL5121343.1"
                     /translation="MQQSEINQIWQAIRDEAKELANCEPMLASFFHATILKHHNLGDA
                     LSYILANKLENPIMPAIALKEIIEEAYRAEPQIIASAACDINAVRTRDPAVDKWSTPL
                     LYLKGFHALQSYRVTHYLWNQGRKALAVYLQNEISVAFDVDIHPAAKVGCGIMFDHAT
                     GIVVGETSVIENDVSILQGVTLGGTGKENGDRHPKIREGVMIGAGAKILGNIEIGRYS
                     KIGANSVVLQPVPDHATAAGVPARIIGKSSEQKPAFDMNQYFEDEQGLFGDGI"
     gene            complement(1734..2744)
                     /gene="gpsA"
                     /locus_tag="PXA33_002038"
     CDS             complement(1734..2744)
                     /gene="gpsA"
                     /locus_tag="PXA33_002038"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDL5121344.1"
                     /translation="MSEIYSAPITVLGAGSYGTALAIALSRNGHKTYLWGHNPDKMAV
                     LASERMNQDFLPDIVFPEALEIVADLATAVQKSKDLLIVVPSHVFSEVLAQIKPFLSQ
                     DHRIMWATKGLERDTGRLLQTVAKEILGEIYPLAVLSGPTFAKELAAGLPTAISLAST
                     DPVFADQMQKRSHCSKAFRVYLNNDIIGVQLGGAIKNVIAIGAGISDGMGFGANARTA
                     LITRGLAEISRLGVSLGADPKTFTGMAGLGDLVLTCTDNQSRNRRFGLALGQGKSAEV
                     AIAEIGQVVEGFYNTKEAYLLAQTQGVEMPIVEQIYQMLFCGKQASDVVKTLLGRERK
                     GE"
     gene            complement(2851..3351)
                     /gene="secB"
                     /locus_tag="PXA33_002039"
     CDS             complement(2851..3351)
                     /gene="secB"
                     /locus_tag="PXA33_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HDL5121345.1"
                     /translation="MSEENQVASTEEATQTPFELQIQRIYVKDVSFEAPNLPTIFQQE
                     WKPQLGFELDTETKEIGEDLYEVTLHITVQTTLEDSNDVAFICEVKQAGVFTIKGLEG
                     IQLAHCLASQCPNVLYPYARELISSLVNRGTFPALNLSPVNFDALFMDYLQRQEESAE
                     EPALAN"
     gene            complement(3372..3824)
                     /locus_tag="PXA33_002040"
     CDS             complement(3372..3824)
                     /locus_tag="PXA33_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDL5121346.1"
                     /translation="MEQLTFAQQLEQFFANHTLMVFAWVALLIAVIFSFYKSATQKYK
                     IVDNAQATQLINKEEAVVVDLRSDEEFRAGHIIDSIHVLPSDIKAAKINQIDKYKARP
                     VIVVDTNDFTSGGVAADLAKQGFSRVYVLKEGIGGWRAASLPTIKKHK"
     gene            complement(3940..5238)
                     /gene="tig"
                     /locus_tag="PXA33_002041"
     CDS             complement(3940..5238)
                     /gene="tig"
                     /locus_tag="PXA33_002041"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HDL5121347.1"
                     /translation="MSFSIETLEGLKRRVTINVPADKIEAAYKEQLKGYAKNARVDGF
                     RKGKVPHSIIEQRFGIAARQDVLSDEMQRAFFEAVIAEKINLAGRPTFTPNNYQPGQD
                     FSFTAEFEVFPEVELKGLENIEVEKPVVEIADADLDKMVDVLRKQQATWSESQEAAKA
                     ESRVTIDFVGSVDGEEFEGGKASDFVLFMGQGRMIPGFEDGIVGRKAGEQFDIDVTFP
                     EEYHAENLKGKAAKFAITLKKVEEMVLPELTEEFVKKFGNAKSVEELRAEIKKNMQRE
                     LKNAVTSRVKTQVINGLIAQNDVEVPAAAVEEEINVLRQQAVQRFGGKPEMAAQLPAE
                     LFEAEAKRRVQVGLLLSSIIGSNELKVDEKRVEEMIADIASAYEQPAEVVAHYAKNRQ
                     LTENIRNVVLEEQAVDAVLAKAKVTEKTMSFDEVMSQQAQ"
     gene            complement(5535..6677)
                     /gene="tuf"
                     /locus_tag="PXA33_002042"
     CDS             complement(5535..6677)
                     /gene="tuf"
                     /locus_tag="PXA33_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDL5121348.1"
                     /translation="MGTIGHVDHGKTTLTAAITTVLAKHFGGAARAFDQIDNAPEEKA
                     RGITINTSHVEYDTATRHYAHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQT
                     REHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGS
                     ALQALNGVAEWEEKILELANHLDTYIPEPERAIDKPFLLPIEDVFSISGRGTVVTGRV
                     ERGIIRTGDEVEIVGIKDTAKTTVTGVEMFRKLLDEGRAGENVGALLRGTKREEIERG
                     QVLAKPGSITPHTDFESEVYVLSKEEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGV
                     EMVMPGDNIKMTVSLIHPIAMDEGLRFAIREGGRTVGAGVVAKIIK"
BASE COUNT         1850 a         1495 c         1223 g         2129 t
ORIGIN      
        1 gcctaagcaa taaggcactt tgaattttag gagtatattt aggagtacaa ataaaatttg
       61 aattaaccaa aacccttatt tatcaatgct tgaagtgata caattcagat gaccccagca
      121 ccaaattata atcctatacg gttcaaccga tacctaaaaa gccttgaaaa tcaatgtttc
      181 aaggcttttt tagtaacatt atgtttgaac cgattcaagt gtagcttcag gttacataaa
      241 gcttaccact catatccaac tttgagtgaa atgaattttt gttcttgtaa tgtatttcct
      301 tttgtaaagc cagcatgtac cgaacttgat acccctttat agtggagtgt aatccctact
      361 tcttgcttaa aataacggcc tatattttga tttaaggaaa attcatttaa cttagtttta
      421 agtgttccgt ggcttgcatc aacaaaatag cttcctaatt caggttttac ctttaattca
      481 ttaaatataa aagttttatt taatgctaaa ccagcttgaa tggccaaaag atctacccta
      541 tcagtctcaa attttgtgtt ctctaattgg gcatttgcag atgttaaatg atgatagcgc
      601 ataccaaatg acggttgtag gttaaaactt gcaacttcca acgttttacc taccgttaat
      661 cctaaagaat atacatttct ggctagtttt atcgtctggc tttgatggct taatttgtta
      721 ttcgcacgcc caatgctacc atcaatccct acataaatgc cattttccca tgttttctta
      781 ccataaaaag aaaacatagt taaatcccct ttaccattat agtagttctg aaaatcatta
      841 aaggttaatt gcaacttgta cttagcaatg tctaaatcat aaattaaagg aataactctt
      901 acctaaatcc catcaccaaa caaaccttgc tcatcttcaa agtattgatt catatcaaaa
      961 gccggttttt gttcagagga tttgccgata atgcgagccg gtacacctgc tgctgtggcg
     1021 tgatcaggca caggttgtag caccactgaa tttgccccga ttttagaata gcgtccgatt
     1081 tcaatattac cgagaatttt tgcaccggct ccaatcatca cgccttcacg gattttcggg
     1141 tggcggtcgc cattttcttt ccctgtaccg cctagtgtta caccttgtaa aatcgagaca
     1201 tcattttcga tcacagaggt ttcacccacc acaataccgg tggcgtggtc aaacataatt
     1261 ccgcaaccga cctttgccgc aggatgaata tccacatcaa aggcaacaga aatttcattt
     1321 tgtaaataaa ccgctaatgc tttcctgcct tgattccaaa gataatgcgt cacccgatag
     1381 ctttgtaaag cgtggaagcc ttttaagtac agcagaggtg tactccattt gtccaccgca
     1441 ggatctctag ttctgaccgc attaatatca caagctgcac tagcaataat ttgtggctct
     1501 gctcgataag cttcctcaat aatttctttt aaggcgattg ccggcataat cgggttttct
     1561 aacttattcg ccaaaatata acttaacgca tcgcctagat tatggtgttt taaaatggta
     1621 gcgtggaaaa aacttgctaa cataggttca caattcgcca gctctttggc ttcatctcga
     1681 attgcttgcc aaatctgatt aatttcactc tgctgcattt tattctcctt tctttattcg
     1741 ccttttcgtt cacgccccaa caaggttttc accacatccg aagcctgttt gccacaaaat
     1801 aacatttggt aaatttgttc tacaatcggc atttcaacgc cctgtgtttg agcgagcaaa
     1861 taggcttctt tggtattata aaatccttct accacttgac cgatttccgc tatcgcgact
     1921 tctgccgatt ttccttgccc taacgccaaa ccaaatcggc ggtttcggga ttggttatcg
     1981 gtacacgtca gcactaaatc tccaagcccc gccattccgg taaaggtttt cggatcagcc
     2041 ccgagcgaaa cacccaaacg gctaatctcc gccagccctc gagtaatcaa ggcggtacga
     2101 gcattcgccc cgaatcccat tccgtctgaa ataccggcac caattgcaat cacattttta
     2161 atagccccac cgagttgcac accgataata tcattgttga ggtaaactcg gaacgctttg
     2221 gaacagtgac tccgtttttg catttgatcg gcaaaaacag gatcagttga ggcaagcgaa
     2281 atcgctgttg gcaagcctgc cgccagttct ttggcaaaag tcggaccgga aagcaccgca
     2341 agcgggtaaa tttcccccaa aatttcctta gctaccgttt gcaacaaacg cccggtatct
     2401 cgctccagcc ctttggttgc ccacattata cggtgatctt gggacaaaaa aggcttgatt
     2461 tgagccaata cttcagagaa tacatgactt ggcaccacaa tcagcagatc tttcgatttt
     2521 tgtaccgctg tagcaagatc tgcgacaatt tctaaggctt ccgggaatac aatatcaggc
     2581 agaaaatctt ggttcattcg ctctgatgcc aaaacggcca ttttgtcggg attatgcccc
     2641 cataaatagg ttttgtgacc atttcgagaa agggcaatgg ctaaagctgt tccataagaa
     2701 cctgcaccta ataccgtgat tggggcagaa tagatttcac tcatatttcc tctgttcggc
     2761 ttatttagaa aattgcaaaa aatagtagaa aaaataaccg cttgtaaaaa aataggtatg
     2821 ccttgcatac ctattttcta tctaaaatgc ttagttcgct aaagccggct cttctgcact
     2881 ttcttcttgg cgttggagat aatccataaa taacgcatca aagtttaccg gtgagagatt
     2941 taatgccggg aatgtaccac gattcacaag gcttgagatt agctcacgag cataaggata
     3001 aagcacattc ggacattgtg aagctaagca gtgagcaagt tgaatgcctt ctaaaccttt
     3061 aatagtaaac acacctgctt gttttacttc acaaatgaaa gcgacatcat tgctgtcttc
     3121 taacgtagtt tgcaccgtaa tgtgtaaggt aacttcgtat aaatcttcac caatttcttt
     3181 ggtttcagta tctaactcaa agcctaattg tggtttccac tcttgttgga aaatagtcgg
     3241 taaatttggt gcttcaaatg aaacatcttt tacataaatg cgttgaattt gaagctcaaa
     3301 aggggtttgt gtagcttctt ctgttgatgc aacttgattt tcttctgaca taaaatttcc
     3361 ttaataatag attatttatg ttttttaata gttggtaggc ttgctgcacg ccagccgcca
     3421 atcccttctt ttaatacata aactctgcta aagccttgtt tagcaagatc tgccgcaaca
     3481 ccacctgaag tgaagtcatt agtgtctact acaattaccg ggcgagcttt gtatttatca
     3541 atctgattga ttttagctgc tttaatgtct gacggtaaca cgtgaatgct atcaataata
     3601 tgaccggcac ggaactcttc atcagaacgt aaatcaacca ctactgcctc ttctttatta
     3661 atcagctgtg tagcctgtgc gttatcgaca attttatatt tttgcgttgc acttttataa
     3721 aagctaaaaa ttaccgcaat caataatgca acccaagcaa acaccatcaa ggtatgattg
     3781 gcaaaaaatt gctctaattg ttgagcaaaa gttagttgtt ccataattca atttccttaa
     3841 catcaaaaaa taagcggtcg ttttgatgca aatttatcac taaatttaac cgcttgtaaa
     3901 agtaataaaa ggtgagcaaa agcccacctt ataaggaaat tattgagctt gttgtgacat
     3961 tacttcgtca aatgacattg ttttttcagt cactttcgct ttcgctagca ctgcatcaac
     4021 cgcttgctct tctaatacca cattgcggat attttcagtt aattgacggt ttttcgcata
     4081 gtgagcaacc acttcagccg gttgttcgta agctgaagcg atatctgcga tcatctcttc
     4141 aacacgtttt tcatctactt ttaattcgtt agaaccgatg attgaagata ataataagcc
     4201 cacttgaaca cggcgtttag cttctgcttc aaataattca gccggtaatt gagctgccat
     4261 ttcaggctta ccaccaaaac gttgcaccgc ttgttggcgt aatacgttga tttcttcttc
     4321 aaccgcagca gccggcactt caacatcatt ttgagcaatt aagccgttga ttacttgagt
     4381 tttcacacgt gaagtgactg catttttaag ttcacgttgc atatttttct taatttctgc
     4441 gcgtaattct tccactgatt ttgcattacc gaattttttc acaaactctt cggttaattc
     4501 cggtaatacc atctcttcta cttttttcaa ggtaattgca aatttcgctg ctttgccttt
     4561 taagttttca gcgtggtatt cttctgggaa agtcacatca atgtcgaatt gctcacccgc
     4621 tttacgacct acgattccgt cttcaaaccc cgggatcata cgaccttgcc ccataaataa
     4681 cacgaagtct gatgctttac cgccttcgaa ttcttcacca tctactgaac caacgaaatc
     4741 aatagttaca cgagattcgg cttttgcagc ctcttggctt tctgaccaag tcgcttgttg
     4801 tttgcgtaat acatctacca ttttgtctaa atcagcgtct gcgatttcaa caaccggttt
     4861 ttctacttcg atattctcta aaccttttaa ttccacttct gggaatactt caaattccgc
     4921 agtaaagctg aagtcttgac ctggttggta gttgtttggc gtgaaagtag ggcgacctgc
     4981 caagttgatt ttttcagcaa tgacggcttc aaagaatgca cgttgcattt catcagataa
     5041 cacatcttga cgggctgcaa taccaaaacg ttgttcgata attgagtgtg gcactttacc
     5101 tttacggaag ccatctacac gagcattttt cgcatagcct tttaattgtt ctttgtatgc
     5161 tgcttcgatt ttatcagctg gtacgttaat agtaacacgg cgttttaagc cttctaatgt
     5221 ttcaattgaa aatgacattc ttaaacctcg gtttagggat ataaaaaaga tatacaaata
     5281 gacggttatt gtagcgttct atcaagaaac tgtcgagaag aaactcattc gcaaataaac
     5341 agttttgtaa aaaatcccta aaatctgact gcttgtcaat gggcttgcct acaagcggtc
     5401 ttttttcacc gatttttgca aatgaaatga tatgatatgg ctagcttaat cggcataaaa
     5461 gcaaaagagg catacctttc gatatgcctc tttctagttt ttatttagct tttcaattta
     5521 tcaaacaatc ttaattattt gatgatttta gctacaacgc ccgcacctac tgtacgacca
     5581 ccttcacgaa tcgcaaaacg taaaccttcg tccatcgcga ttgggtgaat taggctaaca
     5641 gtcattttga tgttgtcgcc cggcattacc atctctacac cttccggtaa ttcgattgta
     5701 cctgttacgt ccgttgtacg gaagtagaac tgtggacggt aacctttgaa gaatggagtg
     5761 tgacgaccac cttcttcttt tgataatacg taaacttctg attcgaagtc tgtgtgtgga
     5821 gtgattgaac ccggtttcgc taatacttga ccacgttcga tttcttcacg tttagtacca
     5881 cgtaataatg cacctacgtt ctcacccgca cgaccttcgt ctaataattt acggaacatc
     5941 tcaacacctg ttacggttgt tttcgctgta tctttgatac ctacgatttc aacttcatca
     6001 ccagtgcgga tgataccacg ctcaacacga cctgttacta ctgtaccacg acctgaaatt
     6061 gagaatacgt cttcgattgg taataagaat ggtttatcaa tcgcacgctc aggctctgga
     6121 atgtatgtgt ctaagtggtt tgctaattca aggattttct cttcccactc tgctacgccg
     6181 tttaacgctt gtaacgctga accacgtacg attggagtgt catcacctgg gaagtcgtat
     6241 tgtgaaagaa gttcacgcac ttccatctca actaattcta ataactcttc gtcatctacc
     6301 atgtcgcatt tgtttaagaa tacgatgatg tatggtacac ctacttggcg acctaataag
     6361 atgtgctcac gagtttgtgg cattggaccg tcagttgctg ctactactaa gatagcgccg
     6421 tccatttgtg ccgcaccggt aatcatgttt ttaacatagt ccgcgtgtcc cgggcagtca
     6481 acgtgtgcgt agtgacgagt tgctgtatcg tactcaacgt gtgaagtgtt gatggtgata
     6541 ccacgcgctt tttcttctgg cgcgttatcg atttggtcaa atgcacgagc tgcaccaccg
     6601 aagtgttttg ctaatacggt agtgattgct gctgttaaag ttgttttacc atggtcaacg
     6661 tggccgattg tacccacatt aacgtgcggt tttgtac
//
LOCUS       DANLRK010000142         6566 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.142, whole genome shotgun sequence.
ACCESSION   DANLRK010000142 DANLRK010000000
VERSION     DANLRK010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6566
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.142"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            84..1511
                     /gene="aspA"
                     /locus_tag="PXA33_002043"
     CDS             84..1511
                     /gene="aspA"
                     /locus_tag="PXA33_002043"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HDL5121349.1"
                     /translation="MSDVRIEHDLLGEREVPNSAYWGIHTLRAIENFNISNVTISDVP
                     EFVRGMVMVKKATALANGELGAIPKKIAKAIVAACDEVLVKGRCLDQFPSDVYQGGAG
                     TSVNMNTNEVIANLALEILGEAKGKYDIVNPMDHVNASQSTNDAYPTGFRIAVYNSIT
                     KLINKITYLQTAFENKATEFSDVLKMGRTQLQDAVPMTLGQEFKAFAVLLDEEVRNLK
                     RTAELLLEVNLGATAIGTGLNTPEGYSTLAVKYLAEVTQLPCSLSENLIEATSDCGAY
                     VMVHGALKRTAVKLSKVCNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPEV
                     VNQVCFKVIGNDTTVTFAAEAGQLQLNVMEPVIGQAMFESIELLSNACINLRDKCIDG
                     ITANRDICQNYVFSSIGIVTYLNPFIGHHNGDIVGKICATTGKSVREVVLERGLLTEE
                     QLDDILSIENLMNPTYKAKRFTEEE"
     gene            complement(1586..3604)
                     /gene="rep"
                     /locus_tag="PXA33_002044"
     CDS             complement(1586..3604)
                     /gene="rep"
                     /locus_tag="PXA33_002044"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HDL5121350.1"
                     /translation="MKLNSQQQQAVEYTKGACLVLAGAGSGKTRVIINKIAHLIAHCG
                     YSPKQIAAVTFTNKAAREMRERVDHSIGKENSKGLTISTFHTLGFEILKREYKLLGFK
                     SGMTLFDEQDQIALLKHLLPENVTEDKDLLKALIATISNWKNDLLSPERVLTRVRDER
                     ERVFSHFYQLYQNQLKAYNALDFDDLIMLPVLLFRQFPEAKSRWQQKIRYLLVDEYQD
                     TNTSQYELIKLIVGNEPNFTVVGDDDQSIYSWRGAKPENMQRLREDFDLQVIKLEQNY
                     RSSQRILHCANILIDNNDHLFSKRLFSELGQGEKLQVIEAKNEEHEAERIVGELIAHR
                     FSKKTKYKDYAILYRGNHQSRLLEKLLMQNRIPYKISGGTSFFARAEIKDMMAYLRLV
                     VNQDDDAAFLRIVNTPKREIGAATLEKLGILANEKQVSLFEAIFDFELIQRLTPKPYQ
                     ALQDFARWIVEIADQADRSDPIEAVKDLLAKIQYEAYLYEMAANPKVAEMQSRNVQTL
                     FEWVQAMLEGDEIEPPMNLVQVVNRLTLRDMLERGEEDDEADQVQLMTLHASKGLEFP
                     HVFLIGMEEGILPHQTSLDEDNVEEERRLAYVGITRAQQTLTFSLCKERRQYGEIIKP
                     EPSRFLLELPQDDLEWESEKPRMTEQEKEAKSSANIANLMAMLKANKK"
     gene            3852..4967
                     /gene="potA"
                     /locus_tag="PXA33_002045"
     CDS             3852..4967
                     /gene="potA"
                     /locus_tag="PXA33_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HDL5121351.1"
                     /translation="MEKVEQKAIIELKNVTKSYGSKTILKNLNLTINDGEFLTILGPS
                     GCGKTTALRLIAGFEDLTEGSIILDGQDVSNVPAEQRPVNTVFQSYALFPHMTIFENV
                     AFGLRMQKVPNAEIEPRVMEALRMVRLEDRAQQKPAQLSGGQQQRIAIARAVVNKPKV
                     LLLDESLSALDYKLRKEMQNELKALQRQLGITFIFVTHDQEEALTMSDRIIVMNAGKI
                     AQDGTPREIYEEPSNLFVARFIGEINVFDATVIERVDATNVKANVEGRICNIKVEDLE
                     VQPNQKLKVLLRPEDIVIEELDENESSKAIIGHIIDRNYKGMTLESSVRLDHNGMNVL
                     VSEFFNEDDPNIDHEVGQKVALTWYEGWEVVLNDEGE"
     gene            4954..5817
                     /gene="potB"
                     /locus_tag="PXA33_002046"
     CDS             4954..5817
                     /gene="potB"
                     /locus_tag="PXA33_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005698572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HDL5121352.1"
                     /translation="MKANNKFQNGAVATIFGWLLLFVLVPNVLVLIISFLTENRQSQY
                     FVDFAFSFDAYKSLFNDTYAAVLWNSLYMAGIATFFCLIIGYPFAFFIAKMPERIRPF
                     LLFLVVLPFWTNSLIRIYGIKIFLGVKGFLNESLMALGIISEPMRLLNSEIAIIIGLV
                     YILLPFMILPLYSSIEKIDNRLLEAAKDLGANAFQRFIKVTIPLTMPGIVAGCLLVLL
                     PAMGMFYVADLLGGGKTPLVGNIIKSLFLNTNQFALGSAVSIALTILMALMLYVYYRA
                     NKLLNKKVELE"
     gene            5817..>6566
                     /gene="potC"
                     /locus_tag="PXA33_002047"
     CDS             5817..>6566
                     /gene="potC"
                     /locus_tag="PXA33_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005822687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HDL5121353.1"
                     /translation="MKKMFRNLFMLVVFAYLYIPIIILVINSFNEDRFGLNWKGFTWN
                     WYERLFSNDTLVQATLNSLTIAFFAATFSTILGALTSIALYRYRFRGKKLVGGMLFVV
                     MMSPDIVMAVSMLVLFMILGIKLGFISLLIAHITFCLPYVVIAISSRLSDFDGKMLEA
                     AKDLGASEVTILRKIILPLALPSIISGWLLSFTISLDDVIVSSFVTSPSYEVLPLKIF
                     SLVKTGVTPEVNALATIMIIFSLTLVVLSQLV"
BASE COUNT         1904 a         1312 c         1305 g         2045 t
ORIGIN      
        1 atttgattta gatcgcatta tttgtctatt aaaatcgtta ttatctacca aaatcgattg
       61 tcatttattt taaggagatg aatatgtcag atgtgcgtat tgaacacgat ttacttggtg
      121 aaagagaagt acctaatagt gcttattggg gcattcatac attaagagcg attgagaatt
      181 ttaatatctc aaatgtcacc atttctgatg tacctgaatt tgtgcgtggc atggtaatgg
      241 taaaaaaagc tactgcgttg gcaaatggtg aattgggtgc aatcccgaaa aaaattgcca
      301 aagctattgt ggcagcctgt gatgaagtat tagtgaaagg acgctgtctc gatcaattcc
      361 cttcagatgt ttatcaaggt ggagccggca catcggtgaa tatgaatacc aatgaagtga
      421 ttgccaacct tgcattagaa attttgggcg aggcaaaagg taaatatgat attgtcaatc
      481 caatggatca cgttaatgca agccaatcca ccaatgatgc ctatccaacc ggtttccgca
      541 tcgcggttta taacagtatc actaaattaa tcaataaaat cacttaccta caaactgcat
      601 ttgaaaataa agccactgag ttttccgatg tactaaaaat gggaagaacg cagttacaag
      661 atgccgtgcc aatgacgctc ggtcaggaat ttaaagcttt tgccgtatta ttagatgaag
      721 aggttcgtaa ccttaaacgg acggcagaat tgcttcttga agtgaattta ggcgcaaccg
      781 ctatcggtac tggtctgaat acgccagagg gttactctac tcttgcagtt aaatatttag
      841 ccgaagtcac gcaactgcct tgctcactgt ctgaaaatct cattgaggca acatctgact
      901 gtggtgctta tgtaatggtt cacggtgcat taaaacgcac cgccgtcaaa ctttctaaag
      961 tgtgtaatga cttacgatta ctttcatcag gcccacgtgc cggcttaaat gaaattaatc
     1021 ttccggaatt acaagccggc tcatctatta tgccggcaaa agtaaaccct gttgtacctg
     1081 aagtcgtaaa ccaagtgtgc tttaaagtga ttggtaacga caccaccgtt acttttgcag
     1141 cagaggccgg tcaattacag cttaacgtaa tggagccggt tatcgggcaa gcaatgtttg
     1201 aatctatcga gctgctttcc aatgcatgta ttaatctacg tgataaatgt attgacggta
     1261 ttaccgctaa ccgtgacatc tgccaaaact atgtgttcag ctctattggt attgtgacct
     1321 acctcaatcc atttatcggc caccataatg gtgatattgt cggcaaaatt tgtgcaacca
     1381 ccggcaaaag tgtacgagaa gttgtgcttg agcgaggctt actcaccgaa gaacaactcg
     1441 atgatattct ctcgattgag aacttaatga atccgactta taaagccaaa cgctttaccg
     1501 aagaggaata atcattgttt acaagcggtc tgattctggc aaaaaattgc aaatttttac
     1561 caaaatcaga ccgcttgtta tcccattatt tcttatttgc tttcagcatc gccattaaat
     1621 ttgcaatatt tgcagaagat tttgcctctt tttcctgttc ggtcattctt ggcttttcac
     1681 tttcccactc caaatcatct tgtggcaatt ccagtaaaaa ccggctcggc tcaggtttga
     1741 tgatttcgcc atattggcga cgttctttac acagtgaaaa cgtgagcgtt tgttgggctc
     1801 gggtaatgcc gacataagcc aaacgccgct cttcttctac attgtcctca tcaaggctgg
     1861 tttggtgtgg cagaatcccc tcttccatac cgattaagaa aacgtgcgga aattccaacc
     1921 ctttcgaggc gtgaagagtc attagttgaa cttgatcggc ttcatcgtcc tcttcgccac
     1981 gttcgagcat atctcgcaag gttaaacggt ttaccacttg caccaaattc atcggaggct
     2041 ctatttcatc gccctctaac atcgcttgca cccattcaaa caaggtctgc acattacggc
     2101 tttgcatttc cgctactttt gggttcgccg ccatttcata caaataggct tcatactgaa
     2161 ttttcgccaa cagatctttt actgcttcaa tcggatcgga tctatcggct tgatcggcaa
     2221 tttccacaat ccagcgggca aaatcctgta aggcttggta aggctttggc gtgaggcgtt
     2281 gtatgagttc aaaatcaaaa atcgcctcaa acaaactgac ctgcttttca ttagcaagta
     2341 tgcctaattt ttctagcgtt gccgccccaa tttcccgttt cggcgtattc acaatacgca
     2401 agaaagctgc atcatcgtct tgattcacta ccaaccgcag atacgccatc atatctttga
     2461 tttccgctcg agcaaaaaat gacgtaccac ccgaaatttt atacggaatg cggttttgca
     2521 ttagcaattt ttccaacaag cgggattgat ggttgcctcg atacaaaatt gcataatctt
     2581 tgtatttggt ctttttcgag aaacgatggg caatcagctc gcccacaatg cgttccgctt
     2641 cgtgttcctc attttttgcc tcaatgacct gtaatttttc cccttgcccc agttctgaaa
     2701 acagccgctt gctaaacaag tgatcgttat tatcaatcag aatattggca caatgcagaa
     2761 tccgctggct ggaacggtaa ttttgctcaa gtttaatcac ttgcaaatca aaatcttccc
     2821 gtaaccgttg catattttca ggcttcgccc cacgccaaga gtaaattgat tggtcatcat
     2881 cgcccacgac cgtaaagttt ggctcgttgc cgacaatcag cttaattaac tcatattggc
     2941 tggtgttggt gtcttgatat tcatcaacta acaaatagcg aattttctgt tgccaacgag
     3001 attttgcttc gggaaattgg cggaacagca acaccggcaa cataatcaaa tcatcaaaat
     3061 ccaacgcatt ataagcctta agttgattct ggtagagctg ataaaaatgt gaaaacactc
     3121 gctcacgttc atcacgcaca cgagtaagta ccctttcagg cgaaagcaaa tcgtttttcc
     3181 agttagaaat agtggcaatt aaggctttca gcagatcctt atcttccgtc acattttcag
     3241 gcaataaatg tttcagcaaa gcaatttgat cctgctcatc aaacaaggtc atacccgatt
     3301 taaaaccgag caatttatat tcgcgtttta aaatttcaaa cccaagtgtg tgaaaagtcg
     3361 aaatggttaa acctttgctg ttttctttgc cgatagaatg atccacccgt tcacgcattt
     3421 cccgagcagc tttgttggta aaggttactg ctgcaatctg cttaggcgaa tagccacaat
     3481 gtgcaattaa atgtgcgatt ttattaataa tcacccgagt cttacccgag ccggcaccag
     3541 ccagcacaag gcaagcccct ttggtatatt ctactgcttg ttgttgttga gaattgagtt
     3601 tcattgaatc tattcaaaag taattttgac aactatttta acggaaagaa aacaagcggt
     3661 cgaattttca ggaaaatttg caaaaaactt tgaaaatccg accgcttgtt catcagagaa
     3721 aaatccccaa aacgcaaatt tttcctttca atcttgcccc tttagctata tcattataag
     3781 ttgatttttt aggggcaaat gcctgatttt ataactcctt ttctttttaa tttaagggaa
     3841 aaaaacagta gatggaaaaa gtagaacaga aagctatcat tgagcttaaa aatgtaacaa
     3901 aaagctacgg tagtaaaacg attcttaaaa atttaaattt aaccattaac gatggtgaat
     3961 ttttaacaat tttaggccct tccggttgtg gtaaaacaac tgcgttacgt ttaattgccg
     4021 gttttgaaga tttaactgaa ggttcaatta ttcttgatgg gcaagatgtt tctaacgttc
     4081 cggcggagca acgcccggta aacaccgtat tccaaagcta tgcccttttc ccacatatga
     4141 cgatttttga aaacgtggct ttcgggctgc gtatgcaaaa agtgccaaat gctgaaattg
     4201 agccacgtgt gatggaagca ttacgaatgg tgcgtttaga agaccgcgct caacaaaaac
     4261 cggctcagct ttccggtggt cagcaacaac gtattgcaat tgcccgtgcg gtggtaaata
     4321 aaccgaaagt attgttgtta gacgaatcgc tttctgcgtt agattataaa ttgcgtaaag
     4381 agatgcaaaa tgaattaaaa gcattgcaac gtcaattagg cattaccttt atttttgtga
     4441 cccacgatca ggaagaagcc ttaaccatgt ctgatcgtat tatcgtaatg aacgctggta
     4501 aaatcgctca agacggtaca ccacgtgaga tttacgaaga gccaagtaac ttatttgtag
     4561 ctcgtttcat cggcgaaatt aacgtttttg atgcaactgt aattgagcgt gttgatgcaa
     4621 ctaatgtaaa agcaaacgta gaaggtcgca tttgcaatat caaagtggaa gatttagagg
     4681 ttcagccgaa ccaaaaatta aaagtattgt tacgccctga agatattgtg attgaggagt
     4741 tagacgaaaa cgaaagctcg aaagcaatta tcggtcatat tatcgaccgt aactacaaag
     4801 gtatgacatt agagtctagc gtgagattag atcataacgg tatgaatgta ttggttagcg
     4861 agttcttcaa tgaagacgat ccgaacattg accacgaagt gggacaaaaa gttgctctta
     4921 cttggtatga aggctgggag gttgtgttaa acgatgaagg cgaataacaa attccaaaat
     4981 ggtgcggtcg caactatttt cggttggtta ttacttttcg tattagtgcc gaacgtactc
     5041 gttttaatca tcagcttctt aacagaaaac cgtcaaagtc agtattttgt ggactttgca
     5101 tttagttttg atgcttataa atcactgttt aatgacacct atgcagcagt attgtggaac
     5161 tcgctatata tggcgggcat tgccaccttt ttctgtttga tcatcggcta tccctttgca
     5221 ttttttattg ccaaaatgcc ggaaagaatt cgtccgtttt tactgttttt agtagttcta
     5281 ccgttttgga ctaactcact gattcgtatt tatggcatta agattttctt aggcgtaaaa
     5341 ggctttttaa atgaaagctt gatggcttta ggtataatca gcgagccaat gcgtttatta
     5401 aactcagaaa ttgcgattat tattggttta gtttacattc tattaccttt tatgattcta
     5461 ccgctttatt catcaatcga gaaaattgat aaccgcttac tagaagctgc aaaagactta
     5521 ggtgcaaacg ccttccagcg ttttattaaa gtgactattc cattaacaat gccgggcatt
     5581 gtcgcaggtt gtttattagt attacttccg gcaatgggta tgttctatgt ggcagattta
     5641 ttaggcggcg gtaaaacacc gttagtaggt aacattatta agagcttgtt cttaaatacc
     5701 aaccaatttg cattaggttc agcagtaagt attgcattaa ctatcttaat ggcattaatg
     5761 ctttatgttt actatcgtgc aaacaaatta ttaaataaaa aggtggagct tgaataatga
     5821 aaaagatgtt tagaaacctt tttatgttgg ttgtttttgc atatctctat attccaatta
     5881 tcattttagt gatcaactcc tttaatgaag accgctttgg attaaattgg aaaggcttta
     5941 cttggaattg gtatgagcgt ttattcagta atgatacgct tgtacaagca acattgaact
     6001 cattaactat tgcgttcttt gcggcaacat tctcaaccat tttaggggca ttaacctcta
     6061 ttgcgcttta ccgttaccgt ttccgtggta aaaagctagt gggcggaatg ttattcgtgg
     6121 taatgatgtc gcctgatatc gtaatggcgg tatctatgct cgtattattt atgatcttag
     6181 gtatcaagct aggctttatt tcgctattaa ttgcacacat tactttctgt ttgccttatg
     6241 tggtaattgc aatttcctca cgcctcagcg attttgacgg caaaatgcta gaagctgcaa
     6301 aagacctagg tgcaagtgaa gtgactattt tgcgtaaaat tattttacca ctggcattac
     6361 cttcgattat ttcaggctgg ttgttaagct ttacgatttc gttagatgac gttattgttt
     6421 catctttcgt gacaagccca agctacgaag ttttaccact gaaaattttc tcattggtta
     6481 aaacaggtgt aacacctgaa gtgaatgcac tagcaactat tatgattatt ttctcattaa
     6541 cattagtggt attaagccag ttagta
//
LOCUS       DANLRK010000143         6538 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.143, whole genome shotgun sequence.
ACCESSION   DANLRK010000143 DANLRK010000000
VERSION     DANLRK010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6538
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.143"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(47..122)
                     /locus_tag="PXA33_002048"
     tRNA            complement(47..122)
                     /locus_tag="PXA33_002048"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(87..89),aa:Asn,seq:gtt)
     gene            complement(238..987)
                     /gene="moeB"
                     /locus_tag="PXA33_002049"
     CDS             complement(238..987)
                     /gene="moeB"
                     /locus_tag="PXA33_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HDL5121354.1"
                     /translation="MELTDHEMLRYNRQIILKSVDFDGQEKLKASRVLIVGLGGLGCA
                     ASQYLASAGVGHLTLVDFDLVSLSNLQRQILHTDATIGQPKVESAKARLAAINPNIQI
                     ETINQRCSDEQLAKLIANVDLVLDCTDNVTVRNQLNLQCFKQKRPLVSGSAIRFEGQV
                     SVFCYQEQEPCYECLSRLFGDATLSCVEAGVIAPIVGIIGSIQALEAIKVLLNIGRTL
                     SGKLLMIDGLHFSVREMALPKQNNCPVCTSY"
     gene            complement(1086..2315)
                     /gene="moeA"
                     /locus_tag="PXA33_002050"
     CDS             complement(1086..2315)
                     /gene="moeA"
                     /locus_tag="PXA33_002050"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HDL5121355.1"
                     /translation="MALLALSDALDNMLARLSTPKLVEQLPLNECANRVLAEDIFSPI
                     NVPNFDNSAMDGYAVRISDLAQNLTLNVAGKAFAGNPFNGEIKAGECVRIMTGALVPK
                     GVDAVVMQEETTLNSDGSVTFNHIPKLGSNIRRIGEDVKQGALVLKKGSLLNVASLPL
                     LASLGIASVPVFVKLKVAILSTGDELTSVGEPLSEGKIYDTNRFAVRLMLEKLNCEIL
                     DFGILPDDPILFEQTFRQAQQQADVLITSGGVSVGEADFTKDVLEKLGEIGFWKIAMK
                     PGKPFAFGKLEQAWFFGLPGNPVSALVTFYQLAQPALAKLSGLSTDKIAKLTQNLTAC
                     SAGKLKKAVGRQDFQRGYYYANEKGELEVRAVGTQGSHIFSAFNESNCFIVLEAERGD
                     VDAGGKVTIQPFNSLMY"
     gene            complement(2337..2954)
                     /locus_tag="PXA33_002051"
     CDS             complement(2337..2954)
                     /locus_tag="PXA33_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006719828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HDL5121356.1"
                     /translation="MFDSLAVQFVVLWAVIDPIGSVPVYLAKTIGLPIEARRKIALNA
                     VLIAAGILIFFLIGGQVLLESMQIPLPAFQAAGGLVLLLFALTMIFGESKPEQEMRIS
                     SNLNELAVYPLAVPSIASPGAMMAIVLLTDNHRFHIGDQIITAGIMLLVLFITYLLLL
                     AANRIQHLIGNAGAAIISRVMGLILAAIAVNNLLIGVRDFFVQIS"
     gene            complement(3022..4233)
                     /locus_tag="PXA33_002052"
     CDS             complement(3022..4233)
                     /locus_tag="PXA33_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL5121357.1"
                     /translation="MISRQMQSLLKRLVAQFPAVLVTGARQVGKSTLLQHIGEGYQYI
                     TFDDPMLLELAKQESRLFFLNHQGKLILDEVQYVPEIFALLKLEIDKKQQNGLFLLSG
                     SQAFELMQNVSETLAGRIAILKLQGLSLREILGVKFDQPFLPTTDYLLAREQELKSPE
                     NIWQLIHKGDMPRLYAQEVDWEIYYRSYVATYIERDVRQLTNIASTTDFTRFMVSIAS
                     RSGELLNYSNIAQEVGVSNETIKRWVAILQTSGIIYLLQPYHNNHLKRAIKTPKVYFL
                     DTGLMAYLTKWLTPETIAQGAKSWQFFETFVVSQIIKSFHNQGIEPPIYFYRDTNQKE
                     IDLIIEYDRTLYPIEIKVTASPTKKMAKSFDLLNSVSSEWKLGQGAIINQYPTKLYLS
                     ENLVALPVSYL"
     gene            4430..6361
                     /locus_tag="PXA33_002053"
     CDS             4430..6361
                     /locus_tag="PXA33_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL5121358.1"
                     /translation="MIFFTDLILKRGHSVLIDNSSVTIHTGQKVGLVGKNGCGKSSLF
                     ALLKNELQPEGGDVSLPKNWAISWVNQETPALAISALDYVIQGDREYTHLMAELDIAN
                     EKNDGNRIAIIHTQLDTIDAWTIQSRAATLLNGLGFSTEQLQLPVKSFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLERWLSNYRGTLLLISHDRDFLDPIIDRVIH
                     IEHQKLNEYTGNYTSFEIQRATKIAQQNAAYQQQQRKVAHLQSFIDRFKAKATKAKQA
                     QSRIKALEKMELIAPAYADSPFEFEFRPPLALPSPLLMMEKVSAGYAEKTVLESVKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELPPQNGSIQLAKGVQLGYFAQHQVDTLRFDE
                     SPLWHLQKLAPEKTEQELRNYLGGFDFKGDKVVQAVKSFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLEMRQALVDALTYYEGSLVVVSHDRHLLRSTVNEFYLVHDRKVEKF
                     KGDLDDYQKWLNEQNALANEPKSVLAKDSANSTACSSTNENSAAQRKEQKRLEAERRQ
                     QTAPLRKKITQLEKELEKATEKLNKLEAMLTESDIYEAENKAKLTETLAKQLEAKRLV
                     EEIEMQWLEAQEEFETMLR"
BASE COUNT         2026 a         1470 c         1267 g         1775 t
ORIGIN      
        1 tataaaagga gacaaataaa acaagaacaa aagaaaaata aaatcttggc tcctcctgcg
       61 ggactcgaac ctgcgacata tggattaaca gtccaccgtt ctaccgactg aactaaggag
      121 gaattaacgg tgtaaataat aggctaaaac gttttagccg tcaaataaaa aaaataaact
      181 gtcgttcatt ttttacattt cttgacattt tttcgccaat aagtatgaga aaaatactta
      241 ataagaggta caaaccggac agttattttg cttaggtaat gccatttctc tgacagaaaa
      301 atgcaagccg tcaatcatta acaattttcc ggaaagagtt ctgccgatat taagtagcac
      361 tttaattgct tccaacgctt gaatactgcc tatgatgcca acaatcggag caatgacccc
      421 cgcttcaaca caacttaaag tcgcatcacc aaacaacctg cttaaacatt catagcacgg
      481 ttcttgttct tgataacaaa atacactcac ctgcccttca aatcgaattg ccgaaccgga
      541 aacaagcggt cgtttttgct taaaacattg caaatttaac tgattacgca cagtaacgtt
      601 atcggtacaa tccagcacta aatctacatt tgcaatcagc ttggctaact gctcatcgga
      661 acatcgctga ttgatagttt caatctgaat gttcggatta atcgccgcta atcgagcttt
      721 ggcagattcc acctttggct gcccgatagt cgcatcggta tgtaaaattt ggcgttgcag
      781 attagaaagt gagaccagat caaaatccac caaagtcaaa tgcccaacgc cggcagaagc
      841 taagtattgc gatgccgcac agcccaaacc acctaaaccg acaatcaaca ctcggctggc
      901 ttttaacttt tcctgcccgt caaaatccac gctttttagg ataatctggc gattataacg
      961 tagcatttcg tgatcggtaa gttccatttt tacacctaaa gagtaaacga tggcgcaagc
     1021 gtccacgctt gtgcctatat ttttattgct agcacaagcg tggacgcttg cgctatctta
     1081 attcattaat acatcagcga attaaacggc tgaatagtga cttttccccc agcatcaaca
     1141 tcgcctcgtt ctgcttctag cacaataaaa cagttacttt cattaaaagc gctgaaaata
     1201 tgtgagcctt gcgtgccaac tgcacgaact tctaactcac ctttttcatt ggcatagtaa
     1261 tagccccgtt ggaaatcttg gcgaccgacc gcttttttca attttccggc actgcaagcg
     1321 gttagatttt gggttaattt tgcaatttta tctgtcgata aacccgaaag tttggctaac
     1381 gccggttgcg ctaattggta gaaagtcact aatgcagaga cagggttgcc cggcagccca
     1441 aaaaaccaag cctgttctaa tttaccgaat gcaaaaggct tgcccggctt catcgcaatt
     1501 ttccagaaac cgatttcgcc taatttttcc agtacatctt tggtaaaatc tgcctctccc
     1561 acagatacgc cgccactggt aatcaacaca tcggcttgct gttgggcttg cctgaaagtt
     1621 tgctcaaaca gaattgggtc atcaggcaaa atcccaaaat ctaaaatctc gcagttgagt
     1681 ttttccagca ttaaacgcac cgcaaaacgg ttggtgtcgt aaatttttcc ctcactcaaa
     1741 ggctcgccaa ctgaggttaa ttcatcgccg gttgaaagaa ttgccacttt taattttaca
     1801 aaaacaggca cagaagcaat accgagtgaa gctagcaacg gtaaactggc tacattcaat
     1861 aatgagcctt ttttcaagac caatgccccc tgtttaacat cttccccaat gcggcgaatg
     1921 ttactgccta attttggaat atggttaaag gttacagagc catcggaatt taacgtagtt
     1981 tcctcttgca ttacaacggc atcaacgccc tttggcacta atgctcccgt cataatccgc
     2041 acgcattcgc ctgcctttat ttcgccgtta aacgggttac cggcaaaggc tttaccagcc
     2101 acattcaagg ttaaattttg tgctaaatct gaaatacgaa ccgcgtagcc gtccatcgcc
     2161 gaattatcaa aattcggtac attaatcggg gaaaaaatat cctctgccaa cactcggtta
     2221 gcacactcat ttaacggaag ttgttccacc aatttaggtg tcgataaacg agcaagcata
     2281 ttatctaacg catcactcaa agccaaaagt gccataattt cctacctatt caacgtttag
     2341 ctaatttgta caaagaaatc ccgtacgcca atcaacagat tattcaccgc aatcgcagcc
     2401 agaattaacc ccatcactcg gctgataatc gcagcccctg cattaccaat aaggtgctga
     2461 atacggtttg ccgctaatag caacaaatag gtaataaaga gtaccaaaag cataatgccg
     2521 gcggtgataa tttgatcgcc gatatgaaaa cggtgattgt cggttaataa tacgattgcc
     2581 atcatcgccc cgggagaagc aatcgacggt acagctagcg gataaaccgc caattcattc
     2641 agattactgc tgatccgcat ttcttgctcg ggcttgcttt cgccaaaaat catcgttaaa
     2701 gcaaataaca acagcactaa gccgcctgcc gcttggaatg caggtaacgg aatctgcatt
     2761 gattctaaca gcacttgccc accgataagg aagaaaatca aaataccggc ggcaatcaag
     2821 accgcattta gtgcaatttt acgccgtgct tcaatcggca aaccaatggt ttttgccaaa
     2881 taaacaggaa cagaaccaat cgggtcgatc accgcccata aaaccacaaa ttgtaccgct
     2941 aatgaatcaa acatattctc tcctgttatc ttgataattt gcaaaatttt agcataaaac
     3001 aaccgcttgc acggcttttt cttacagata gctcaccggt aacgctacca aattttcact
     3061 taaataaagt ttggtcggat attggttaat aatcgctccc tgccctaatt tccattcact
     3121 agaaactgag tttagcaagt caaaactttt tgccattttt ttggttgggc ttgccgtgac
     3181 cttaatttct atcggataaa gcgtgcgatc atattcaata attaagtcga tctctttctg
     3241 gttagtatca cggtaaaaat aaatcggcgg ctcaattcct tgattatgaa aggattttat
     3301 aatttggctt accacaaagg tttcaaagaa ctgccagctt tttgcccctt gagcaatagt
     3361 ttcgggggta agccatttgg ttaaatacgc cattaacccg gtgtctagaa aatacacttt
     3421 tggggtctta attgcccgtt tcaaatgatt gttgtggtat ggctggagca aatagataat
     3481 gccggaagtc tgcaaaatgg cgacccaacg tttaatcgtt tcattcgata ctccaacttc
     3541 ttgagcaata ttactgtaat tgagtagctc accactacga gaggcaatag agaccataaa
     3601 acgggtaaaa tccgttgtgc tggcaatatt ggttaattgg cgaacatctc gctcaatgta
     3661 ggtcgctaca taagagcgat aataaatctc ccaatccacc tcttgtgcat ataaacgagg
     3721 catatcgcct ttatggataa gctgccagat gttctccggt gattttaatt cttgctcccg
     3781 agctaacaaa tagtcggttg tcggtaaaaa aggttgatcg aacttcaccc ctaaaatttc
     3841 acgcaacgaa agcccttgta attttaaaat agcaatacga ccggcaagtg tttcactcac
     3901 attctgcatt aattcaaagg cttgcgaccc cgaaagtaaa aacaaaccgt tttgctgttt
     3961 tttatcaatt tctaacttta ataacgcaaa aatttcaggc acatactgta cttcgtctaa
     4021 aataagcttg ccttgatggt ttaaaaaaaa caggcgagat tcttgtttag ccagctccag
     4081 caacattggg tcatcaaaag taatatattg atagccctca ccaatatgtt gtaacaatgt
     4141 cgatttacca acctgtcttg cccctgtcac taaaacggca ggaaattgcg ccacgagtcg
     4201 ttttaacagc gattgcattt gccgagaaat catttttcct ccaaaatagc ctaaatcaag
     4261 tttaacctca tatataatgt tgaattaaac ttaatttaaa ttatttttta agtttaattc
     4321 aacacagaat attgaattaa actgaatttc ttattttagt tcacaaagca atggctttgc
     4381 ttttttatgc taaaatacgc aaaattttta acaaaataga ccgcttgcaa tgatcttttt
     4441 tacagactta attttaaaac gagggcattc cgttctcatt gataacagct ccgttaccat
     4501 tcacaccggg cagaaagtcg gtttggtggg aaaaaacggc tgtggcaaat cctctctttt
     4561 tgccttactg aaaaatgaat tacaacctga aggtggcgat gtatcgttgc cgaaaaactg
     4621 ggcgatctcg tgggtaaatc aagaaacgcc tgcgttagct atttcagcgt tagactatgt
     4681 gattcaagga gatcgggaat atacccatct tatggctgag ctagatatcg caaatgaaaa
     4741 gaatgacggc aaccgcattg cgattatcca tactcaactt gataccattg atgcttggac
     4801 aattcaatcc cgtgccgcta ccctgcttaa cggcttgggg tttagcaccg aacaactgca
     4861 actgccggtg aaatccttct ccggtggctg gcgtatgcgg ttaaatttgg cacaagctct
     4921 gatttgtcgt tccgatctgt tattgcttga cgagccaacc aaccacttag acttagatgc
     4981 ggttatttgg ttggagcgtt ggctgagtaa ctatcgtggc actttattgt taatttcgca
     5041 cgaccgtgat tttctcgatc cgattatcga tcgggtgatt catattgaac atcaaaagct
     5101 caatgaatac accggcaatt acacctcgtt tgagattcaa cgtgcgacca aaatcgccca
     5161 gcagaatgcc gcttatcagc aacagcaacg taaagttgct catttgcaga gttttatcga
     5221 tcgctttaaa gccaaagcga ctaaagcgaa acaggcacaa agccgaatta aagcgttgga
     5281 aaaaatggag cttattgccc cagcctatgc tgattcgccg tttgaatttg aatttcgccc
     5341 accgcttgca ctgcctagcc cgttattaat gatggaaaaa gtcagtgccg gctatgcgga
     5401 aaaaacggtg ttggaatcgg tcaaactgaa tttggttccc ggctctcgta ttggtttact
     5461 cgggagaaac ggtgcaggga aatccacgct gattaaatta ttggcgggcg aacttccgcc
     5521 ccaaaacggc tcaattcaac tggcaaaagg cgtgcaactc ggctatttcg cccaacatca
     5581 ggtggataca ctacgttttg atgaaagccc actttggcat ttacaaaaac tcgcaccgga
     5641 aaaaaccgag caggaattac gcaattattt aggcggtttc gattttaaag gcgataaagt
     5701 ggtacaagct gtgaaatcat tctccggcgg agaaaaggcc cgtttagtgc ttgccttaat
     5761 tgtatggcaa cgcccgaatt tactgctatt agatgagcca accaaccatt tagatttaga
     5821 aatgcgacaa gccctggttg atgcactgac ctattacgaa ggctcgctag tggtggtttc
     5881 gcacgaccgc catttattgc gtagcaccgt gaatgaattt tatttagtgc acgaccgcaa
     5941 agtggaaaaa tttaaaggtg atttagacga ttaccaaaaa tggctcaatg agcaaaacgc
     6001 cttagccaac gagccgaaat ccgtacttgc aaaagattcg gcaaattcga ccgcttgctc
     6061 tagcaccaat gaaaatagtg cagctcaacg caaagagcaa aaacgcttag aggcagagcg
     6121 ccgccaacaa accgccccat tgcggaaaaa aattactcaa ctggaaaaag agttagaaaa
     6181 agcaaccgaa aaattaaata aactcgaagc aatgcttacc gaaagcgaca tttatgaagc
     6241 cgaaaataaa gcaaaactaa ctgaaacact tgcgaaacag ttagaggcaa aaaggctggt
     6301 tgaagaaatt gaaatgcaat ggttggaggc tcaggaggag tttgagacca tgttacgcta
     6361 gggttctaca agatagcgca agcgtccacg cttgtgccat ttaagggaac tatacacctc
     6421 aaaaaaacaa tgatcattaa gctagctttt ttataaattt aaaaacctat agattaaata
     6481 aataatgata ttaatagtaa aatcacgagt gcaactacac tgacgataat aaaatcag
//
LOCUS       DANLRK010000144         6505 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.144, whole genome shotgun sequence.
ACCESSION   DANLRK010000144 DANLRK010000000
VERSION     DANLRK010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6505
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.144"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            107..2476
                     /gene="malP"
                     /locus_tag="PXA33_002054"
     CDS             107..2476
                     /gene="malP"
                     /locus_tag="PXA33_002054"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HDL5121359.1"
                     /translation="MTFNSIVEKYCRYFDVTDPKHFTLQQWYQVVAEGSLELIYSQPA
                     TKPTESRHVNYLSMEFLIGRLTGNNLMNLGYYEQIRDYLKQYQVELVDVLEQERDPAL
                     GNGGLGRLAACFLDSMATVGQNATGYGLHYQYGLFKQSFKEGMQKEAADTWARDSYPW
                     HRFNPSKTRHIGFGGKIKHIQADQYEWQPKLTIQGKAFDLPIVGYKNNIIQPLRLWQA
                     DSDQSFDFDAFNEGKFLSADKTIVNAAALTQVLYPNDNHQAGQKLRLMQQYFHCACSV
                     ADILDRHISEGYSLEDLAKRQVIQLNDTHPTLAIPELMRVLLDEHQFTWEQAWDICSN
                     TFAYTNHTLLPEALEQWDQRLFKQLLPRHYQIVEKINDIFHDKVQAEFGDDAKIWEKL
                     AVLFDYRVRMANLCVVTCFRVNGVAEIHSDLLVTDLFPEYHKLFPTKFCNVTNGITPR
                     RWIRQANPQLSDLLDRTLKQDWTHNLELLKGVEQYADDTGFREEYQRIKRNNKVVLAN
                     EIHKTLGLNVNPNAIFDVQIKRFHEYKRQHLNLLNIIATYQSLKANPNQDYTPRLFLF
                     SGKAAPGYYLAKNIIHAINNVADIINNDKQVNDRLQVAFLPDYRVSLAEKIIPAADVS
                     EQISMAGKEASGTGNMKLALNGALTLGTLDGANVEIADMVGEENVFIFGHTVESVREL
                     LAKGYQPKAYYNQDPILKNAVDFLATGKASNGDKEAFKLMLDSLLERDPFLVFADFDS
                     YRQAQEKIGVAYLDQETWLRSAILNTARLGAFSSDRSIKDYQQRIWLNR"
     gene            2558..4636
                     /gene="malQ"
                     /locus_tag="PXA33_002055"
     CDS             2558..4636
                     /gene="malQ"
                     /locus_tag="PXA33_002055"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HDL5121360.1"
                     /translation="MKITAKQYYRAGIMPYFFDERGVKKTASEKIKHSLFKAFNGNTQ
                     ARETPIPTVNIFYQHQPCFLPFHLADKKHPLTGTWQLQLENSNQIISGKIKARGITLP
                     QDLPLGYHQLQLKTVDKTFNCTIIIAPERCYQPQAVIEQKRLWGTFLQLYTLKSAHNW
                     GIGDFGDLKDFIQQIAPYQADFIGLNPIHSLFPANPNSASPYSPSSRRWLNIAYIDIN
                     QLAEFQQSDEAQTWFHSAEIQQQLSELRTQDWLNYEQIIPLKLKGLRFAFAKFQQNMT
                     ACAAPAFIEFVQKGGESLQVQATFDALHQHLSAQFADQWGWDFWEKAYQDYHSAAVQQ
                     FRETHKTDIEFYLWLQFVADQQLAECDAICRQQNMRIGMYRDLAVGVSPNGAETWNDK
                     QLYCLNASVGAPPDVLGPQGQNWGLTPMNPHVLKQQAYRPFIELVRANMSHCGALRLD
                     HIMSLLRLWWIPKGDSAVNGAYIRYPVDDLIAILALESQRHQCLIIGEDLGTVPKEIV
                     SKLKNAGILSYKIFYFEFDEQGKSRDLQHYPYQAMTTLSTHDLPTINGYWRGYDFELG
                     EKFGVYPNSNILAILKQDRVNAKAKILQRLQEHNVEIAPEISEQLNSPVNTAFIHCLQ
                     TYVANVSSGLFGFQPEDWLGMTEPVNIPGTSTQYANWRRRLSVNLEEIFTDKDIQALL
                     KEVNLIRKNQ"
     gene            complement(4701..6362)
                     /locus_tag="PXA33_002056"
     CDS             complement(4701..6362)
                     /locus_tag="PXA33_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HDL5121361.1"
                     /translation="MSEIALTVSLLSLVAVLGLWIGHIKIRGVGLGIGGVLFGGLFVS
                     HFMAQFDVKLDAHTLHFIQEFGLILFVYSIGIQVGPGFFASLKHSGLKLNGFAVLIVL
                     ISGILVILIHKFFNVPLPVILGIFSGAVTNTPSLGAGQQVLAELSAESVTEIMGMSYA
                     IAYPFGIIGILLSMWLIRILFRINIEAEAEAFNKQQNRSKDQLSTLNVRLTNPNIHGL
                     TLKQIPDFNLHEVAYSRLKRGNELSIPRVDTILQIGDILHLVGEESSLRKMQIILGEA
                     VDVSVSTKGTIYRSERAVVTNEKVFGKQIRQLMLKGKYDVVISRLNRAGVELVPTGDM
                     TLQFGDVLNLVGRQADIEMVMTIIGNAHSKLQQVQMLPIFIGIGLGVLLGSVPIYLPG
                     FPVALKLGLAGGPLVVALILARIGSFSKLYWFMPPSANLALREIGIVLFLAVVGLKSG
                     GRFLETLLSSEGVEWICYGAIITFVPLMIAGLIARMYNKVNYLTICGLLAGSMTDPPA
                     LAFANEIKEGNGAAALSYATVYPLVMFLRIMLPQVLAIMLWVSGG"
BASE COUNT         2093 a         1402 c         1213 g         1797 t
ORIGIN      
        1 ctcactactt ccttataatc ataccataca agatgattat tgggatgatt tgattttatt
       61 tggattttac ataatagcga cattcatctt taaccggggg tttcctatga catttaattc
      121 tattgtagaa aaatactgtc gctactttga tgtgacagat ccaaaacact ttaccttaca
      181 gcagtggtat caagttgttg ctgaaggttc ccttgagcta atttatagcc agcctgcaac
      241 caaaccgaca gaatcacgcc acgtgaacta cctctcaatg gagttcttaa tcggtcggtt
      301 aaccggcaat aatttaatga atttaggtta ttacgaacaa attcgtgact atcttaaaca
      361 atatcaagtt gaattagtag atgttttaga acaagagcgt gatccggcat tgggtaatgg
      421 cggattaggt cgtcttgctg cctgcttttt agattcgatg gcaactgtcg gacaaaatgc
      481 aaccggctat gggcttcatt atcaatatgg tctgtttaaa caaagcttca aagagggaat
      541 gcagaaagaa gcagcggata cttgggctcg tgacagctac ccgtggcatc gttttaatcc
      601 gtctaaaacc cgccatatcg ggtttggtgg aaaaatcaaa catatccaag ccgatcaata
      661 tgaatggcag cctaaattga ccattcaagg caaagccttt gacttgccga ttgtaggtta
      721 taaaaacaac atcattcaac cattacgttt atggcaagca gatagtgacc aatcttttga
      781 ttttgatgcc tttaatgagg gtaaattttt aagtgccgat aaaacgattg tcaatgcagc
      841 cgcactaact caagtgctgt atccaaacga caaccatcaa gccggacaaa aattacgctt
      901 aatgcagcaa tacttccatt gtgcctgctc ggttgccgat attttagatc gtcacattag
      961 cgaaggttat tcgttagaag atttagcaaa acgccaagtt attcaactca atgataccca
     1021 tccaacgctc gctatacctg aattgatgcg tgtactgtta gatgaacatc aattcacttg
     1081 ggagcaggct tgggatattt gcagcaatac ctttgcttac actaaccata ccttattacc
     1141 tgaagcactt gaacaatggg atcaacgcct atttaagcag ctattacctc gccattatca
     1201 aattgtggaa aaaatcaatg atattttcca cgacaaagta caggcagaat ttggtgatga
     1261 tgcaaaaatt tgggaaaaat tagccgtgct atttgattac cgtgtgcgta tggcaaacct
     1321 ctgcgttgtc acctgcttcc gtgtaaacgg cgttgcggaa attcactccg atctcttagt
     1381 gaccgatcta ttcccggaat accacaaatt attcccaaca aaattctgca atgttactaa
     1441 cggcatcaca cctcgccgct ggattcgaca agctaatcca caattaagtg atttattgga
     1501 tcgtacctta aaacaagatt ggacacacaa tctggaatta ttaaaaggcg ttgaacaata
     1561 tgcggatgac accggtttcc gtgaagaata tcaacgcatt aaacgcaata acaaagtggt
     1621 tttagccaat gaaatccata aaacattagg gttaaatgtc aatcctaatg cgatttttga
     1681 tgtgcaaatt aagcgtttcc acgaatataa acgccaacat ttgaatttgt taaacattat
     1741 tgcgacctat caatcgctaa aagctaaccc gaaccaagac tacacaccac gtctattcct
     1801 attctcgggc aaagctgcac cgggttatta tttagcaaaa aatattattc acgctattaa
     1861 taatgtggca gatattatta ataacgacaa gcaggtaaat gatcgactac aagtggcatt
     1921 cttaccggat tatcgagtca gtcttgcgga aaaaattatt ccagcagctg atgtatcaga
     1981 acagatttca atggcaggta aagaagcctc cggtaccggt aatatgaaat tggcactcaa
     2041 cggcgcatta accttaggta cgctagacgg tgccaatgtt gaaattgctg atatggtcgg
     2101 tgaggagaat gtctttatct tcggtcacac cgtagaaagc gtgagggaat tattagcaaa
     2161 aggctaccaa ccgaaagcct actataacca agatccgatt ctgaaaaatg cggttgattt
     2221 cttagccaca ggaaaagcct ctaacggaga taaagaggca ttcaagctga tgttagatag
     2281 cctgttggaa cgagatcctt tcttagtctt tgccgacttt gatagctatc gccaagcaca
     2341 agagaaaatt ggtgtagcct atttagatca agaaacttgg ctgcgtagtg caattttaaa
     2401 tactgctcgt ttgggtgcat ttagctcaga ccgctcaatt aaagattatc aacaacgtat
     2461 ttggttgaat agataataaa ttcgggcgac ttctacaata aaggaagtcg cctattttct
     2521 gctgtatttc atatgtattt atatatggag agaaatgatg aaaataactg ccaaacaata
     2581 ttatcgggct ggaattatgc cctatttttt cgatgagcgt ggtgtaaaaa aaacagcatc
     2641 tgaaaaaata aaacattcgt tattcaaagc ctttaacggc aatactcaag caagagaaac
     2701 acctatccct acggtcaaca ttttttacca gcaccaaccc tgttttttac cttttcattt
     2761 agcggataaa aaacatccgt taaccggtac ttggcagcta caactagaaa attcaaacca
     2821 aattatttcc ggcaaaatta aagcaagggg aattacatta cctcaagatt tgcccttagg
     2881 ttatcaccaa ttacagctaa aaacggtaga taaaaccttt aattgcacca ttattatcgc
     2941 tccggaacgc tgctaccaac cacaagccgt cattgaacaa aaaagattgt ggggaacctt
     3001 cctacaacta tacaccttaa aatcagccca caactggggg attggcgatt ttggcgattt
     3061 aaaagacttc attcaacaga ttgcccctta ccaagcagat tttatcggct tgaacccgat
     3121 tcactcactt tttccggcaa acccgaattc tgccagccca tacagtccat cctctcgccg
     3181 atggctgaat attgcttaca ttgatatcaa tcagctagcg gaatttcaac aaagtgatga
     3241 agcacaaact tggtttcatt ctgctgaaat ccaacaacaa ttatcagaat tacgcactca
     3301 ggattggttg aattacgagc aaatcattcc gttgaaattg aaagggctac gctttgcttt
     3361 cgcaaaattt cagcaaaata tgaccgcttg cgctgcccct gcttttatcg aatttgtaca
     3421 aaaaggtggt gaaagcttac aagttcaagc cacatttgat gcccttcatc agcatctatc
     3481 ggcacaattt gccgaccaat ggggctggga tttctgggag aaagcctacc aagactacca
     3541 ctccgcagcc gtgcagcaat ttagagaaac gcataaaacc gacatagaat tttacctttg
     3601 gttacaattc gttgccgatc aacagctagc agaatgtgat gctatctgcc gtcagcaaaa
     3661 tatgcgtatt ggtatgtatc gcgatctcgc cgttggcgtc agcccgaatg gtgcagaaac
     3721 gtggaatgac aaacaacttt attgcttaaa tgcctctgtc ggcgctccgc ctgatgtatt
     3781 aggtccgcaa ggacaaaact ggggcttaac cccaatgaat ccgcatgtat taaagcaaca
     3841 agcctaccgt ccgtttattg agttagtcag agctaatatg agtcattgcg gtgcattacg
     3901 ccttgaccat attatgtctt tactccgttt atggtggatc cccaaaggcg atagtgcggt
     3961 taatggtgct tatattcgtt atccagttga tgatttaatc gcaattttgg cacttgaaag
     4021 ccaacgccac caatgcctaa ttatcggtga agatttaggt actgtgccaa aagaaattgt
     4081 gagcaagctc aaaaatgcgg ggattttatc ttacaaaatt ttctattttg aatttgatga
     4141 acaagggaag agtcgagact tacagcatta cccttaccaa gcaatgacca cattaagcac
     4201 ccacgattta ccgactatca atggttattg gcgtggctat gattttgaat taggcgaaaa
     4261 attcggcgtc tatcctaatt cgaatatttt ggcaatcctt aagcaagatc gagtcaatgc
     4321 caaagccaaa atcttacaac gcttacaaga acataacgta gagatcgcac ctgaaattag
     4381 cgagcaactt aattctccgg tgaataccgc atttattcac tgtttacaaa cctatgttgc
     4441 aaatgtaagc agcggattat tcggcttcca acccgaagac tggttgggta tgactgagcc
     4501 ggttaacatt ccgggaacca gtacacaata tgcaaactgg cgacgtcggc ttagtgttaa
     4561 cctcgaggag atcttcacag ataaagatat tcaagcctta ttaaaagaag ttaatttaat
     4621 ccgtaaaaat caataaaagt tagataagaa aaaaggcgac tttccgtcgc cttttgggct
     4681 tatatgcttg atgtctatct ttatccacct gaaacccaca acataatcgc tagcacttga
     4741 ggtagcataa tacgcaaaaa cattactagt gggtaaacag ttgcatagga taacgctgca
     4801 gcgccatttc cctctttaat ttcattggca aaagcaagcg ctggaggatc agtcattgag
     4861 ccggcaagta atccacaaat cgttaaatag tttactttgt tatacattct ggcaatcaat
     4921 cctgctatca tcaatggaac aaaagtaata atcgccccat agcatatcca ctcaacgccc
     4981 tcactgctta atagcgtttc gagaaatcta ccgccagatt ttaaaccaac taccgctaaa
     5041 aacagtacaa taccgatttc acgcaatgca agattggcac ttggtggcat aaaccagtag
     5101 agtttactga aactaccaat tcttgcaaga ataagagcaa ccaccagtgg tccaccagcc
     5161 aagcccagct ttaacgcaac agggaaaccc ggtaaataaa tcggtacaga accaagtaat
     5221 actcccaaac cgatcccgat aaatattggc aacatttgga cttgttgcaa ctttgagtgc
     5281 gcattgccga taatcgtcat taccatttca atatcagctt gccgccctac taaattgaga
     5341 acatcaccaa attgtaacgt catatcacct gttggtacta attctacacc ggctcgattt
     5401 aaacgagaaa tgacaacatc gtatttgcct ttaagcatta gctgacgaat ttgtttcccg
     5461 aagacttttt cattcgtcac tacagcacgt tcagaacggt aaatcgtgcc tttggttgag
     5521 acagatacat ctactgcctc acctaaaata atctgcattt ttcgtaatga ggattcttca
     5581 cctactaagt gtaaaatatc tcctatctgt agaatcgtat ctacacgtgg aatagaaagc
     5641 tcattccccc gttttaagcg tgaataagct acttcgtgca aattaaaatc aggaatttgt
     5701 ttcaacgtta aaccgtgaat atttggatta gtcagacgga catttaaggt actaagttga
     5761 tctttacttc tattttgctg tttgttaaaa gcctctgctt ccgcctcaat attgattcga
     5821 aataaaatcc gaatcagcca cattgaaagt agaattccga taatgccgaa cgggtaagca
     5881 atcgcataac tcattcccat aatttcagtt acactctcag cgcttaactc agccaatacc
     5941 tgttgacctg ctcctaaaga aggggtattg gttacagccc cggagaaaat gcctaaaatc
     6001 acaggcaaag gaacattgaa gaatttatga ataagaataa ctaaaatacc gctaatcagt
     6061 acaatcagta ccgcaaaacc attgagtttt aagccagaat gttttagtga ggcaaagaat
     6121 ccaggtccta cttgaattcc aattgaataa acaaataaaa tcagcccgaa ctcttgaata
     6181 aaatgaagcg tatgggcatc gagcttcaca tcgaattgcg ccataaaatg ggagacaaac
     6241 agcccaccaa ataacactcc ccctatacct agccccacgc ctctgatttt aatatggcca
     6301 atccataagc ctagaacggc cactaaagat aataaactga cagtaagtgc aatttcgctc
     6361 attacagcct ctctttaata tgaaatacct caaaaatggt atccaattct accaaatctc
     6421 aaaaacgaaa aatgagatct atgtcaaaaa aataaaatat tgatagtatt actccaagat
     6481 gaacttcaaa aaagttcctg aattt
//
LOCUS       DANLRK010000145         6491 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.145, whole genome shotgun sequence.
ACCESSION   DANLRK010000145 DANLRK010000000
VERSION     DANLRK010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6491
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.145"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(<1..707)
                     /locus_tag="PXA33_002057"
     CDS             complement(<1..707)
                     /locus_tag="PXA33_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005712160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL5121362.1"
                     /translation="MSIYRRKANGPWWVDITTPNGKRIKRSTRTLVKREAQQYHDKLK
                     QEMWAEDKLEEKRKYIFEDALLHYVRAAEELKDKATKKRHAEYWLSKFAGRELSSLTA
                     QEIILNIPKKNANTKKPLSHSTQNKYVKSLSRVLNIAHKLGMLDAVPHIPKKKEPPIR
                     VRWITKEQAKQLIDKLSSDWMKSICKFALMTGARRTEILTMTWDKIDLVRKVAIVTND
                     VAKSGKARSLLLNQEAI"
     gene            complement(611..934)
                     /locus_tag="PXA33_002058"
     CDS             complement(611..934)
                     /locus_tag="PXA33_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005822951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL5121363.1"
                     /translation="MEHLTINQVAKELNCHPNTVRQHLADWGFFKMVGSEVWRINRED
                     LERAKKRAGIDPIRKRSDNDRGLSLSVEENEKCRSTDEKQTVRGGLISQPQTARELRE
                     ALARL"
     gene            complement(1015..1182)
                     /locus_tag="PXA33_002059"
     CDS             complement(1015..1182)
                     /locus_tag="PXA33_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HDL5121364.1"
                     /translation="MQFTYGSICSGIEATSIAWHGLGKPLWFSEIEPFPCAVLAHRFP
                     NVPNGNGMAVP"
     gene            complement(1190..1564)
                     /locus_tag="PXA33_002060"
     CDS             complement(1190..1564)
                     /locus_tag="PXA33_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121365.1"
                     /translation="MHIEDKIAWWLANGETGVSSKTMAFYLGYGIRPKIEGYPHDVSD
                     FRRCFLLLETVPFLRNRIEKMAELGKVWAALAKEWHTLEALYNEEECQIRCPKTYAKL
                     REILEANEENVVRIGNLSISIG"
     gene            1714..1941
                     /locus_tag="PXA33_002061"
                     /pseudo
     CDS             1714..1941
                     /locus_tag="PXA33_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005609911.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
     gene            1966..2361
                     /locus_tag="PXA33_002062"
     CDS             1966..2361
                     /locus_tag="PXA33_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL5121366.1"
                     /translation="MKIHTQIRKEILSLLEAKLTDIEHFYNGQPNFIDIDEQQLAISV
                     YLDEINRQELTLCDEQWTAQLNITIYLKSVDEAEDELDEWAEKIREVVETYSAFEHLE
                     GISLSQYQYEQDQNQRTWHSATLIFDVEY"
     gene            2393..3034
                     /locus_tag="PXA33_002063"
     CDS             2393..3034
                     /locus_tag="PXA33_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121367.1"
                     /translation="MAKTTKVQGTKFRIGIGREAQKAITAITLATATLTIATSGYKKG
                     DAIEITGCGQLDGIYPVLSVTGDQVKLCEEVNWTGKDLPANYTKAKAALVQYSDQFCA
                     VKNIEKSDDTLSTEDVTTVCSEGTETEPGEIEFGSIKLSFFHKPSTEMQTRLRKLFYD
                     KSTFAYKLELPDNHGTTYGEGFIEAGNGFSGEVKGKYEGSVSIKPSKRDYLLV"
     gene            3117..3518
                     /locus_tag="PXA33_002064"
     CDS             3117..3518
                     /locus_tag="PXA33_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121368.1"
                     /translation="MTLREKLLANKPKLQSIEINGETYYLREATVGDMNKQIFETRSW
                     LIQQAEQENVELPAEDDETFDEALNRFGEKYRLAQSVAYRLCDENGALLFNPLNIDDL
                     NAIAELDSKVIIDFNQAVSAPKDSASEESSK"
     gene            3563..3793
                     /locus_tag="PXA33_002065"
     CDS             3563..3793
                     /locus_tag="PXA33_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HDL5121369.1"
                     /translation="MPERHFAEYQLFYQEQPFGLWREDYRAAQLAHLTAMINRDPKGK
                     APELSEFMPFFNRADEVEANEDDGVADYLAKR"
     gene            3861..4127
                     /locus_tag="PXA33_002066"
     CDS             3861..4127
                     /locus_tag="PXA33_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HDL5121370.1"
                     /translation="MATINDAFSFRTNTEIKNTAFDVIKNYGMTPSQVFNMFLTEIAK
                     TKTIPLSLNYQPNLETKLAMQEAKSGKNEVYASLEAFHKAMLAE"
     gene            4131..4400
                     /locus_tag="PXA33_002067"
     CDS             4131..4400
                     /locus_tag="PXA33_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin, RelE/StbE family"
                     /protein_id="HDL5121371.1"
                     /translation="MLQISPTNAYKRDFKKIAAELVGSSEYVEVMYCLINQLPLAEKY
                     RDHPLQGEWQGFRDCHIKPDLVLIYAVEDNLLRLVRLGSHAELFG"
     gene            4475..4738
                     /locus_tag="PXA33_002068"
     CDS             4475..4738
                     /locus_tag="PXA33_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121372.1"
                     /translation="MKNVLLFIVHFFLIAMLSIIPILIILGAIERDPYYTAWIFLSVF
                     LGIGALLYFATIVSKLINKGKTQTLEEKRYAEYWNNVKVNIPK"
     gene            4798..5508
                     /locus_tag="PXA33_002069"
     CDS             4798..5508
                     /locus_tag="PXA33_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HDL5121373.1"
                     /translation="MSGLGKLTVTLELENAKFQSAMTKSDYEAQKFAKNFIQNMDRAR
                     NHAKEFADRSTQYLKNIEQAAKNLNKNSEFSFLSTIGGHYQSMSSGILSAAKAYTDIQ
                     NKMKLVSGSSAEAAKRLTDVFDIATKTSQSTEAVSGVYQTFAQNAQSLGLAQKDVAEL
                     TKTVSQAVAASGASSSAASNALTQFGQSLLMGKMKAQEFNSLITQTPTIIQAMAKGLG
                     MTMAEFKAAVYRAKSLLG"
     gene            5486..5776
                     /locus_tag="PXA33_002070"
                     /pseudo
     CDS             5486..5776
                     /locus_tag="PXA33_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484718.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            5766..6485
                     /locus_tag="PXA33_002071"
     CDS             5766..6485
                     /locus_tag="PXA33_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HDL5121374.1"
                     /translation="MKFSKYQLLVNKICFICGKKDCPQMKKSKDYKDFLDALEKGDVD
                     KADKIYNTKFSQFSKSFGHEMEKNLEKNRIPPIYQGVPFDSFDLYQHYSYGNGEALNL
                     SSVGLSNKIRPLVNKDKAFGKSNGSIESRFISQYKNEGRTSFSNAYDFTKEASGIADP
                     LWALGTATISGELKDIQIFPGGIARGNIHYSIRDRFTDPFDTFNFTKGEWNPNGTPYI
                     IQDSWIKSVRFSIDKKIKITP"
BASE COUNT         2068 a         1279 c         1251 g         1893 t
ORIGIN      
        1 ttaattgcct cttgattcag taatagcgat cgtgccttac ctgatttagc gacatcatta
       61 gtcacgattg ctacctttct tacaagatct atcttatccc aagtcattgt caaaatttct
      121 gtccgtctgg ctccagtcat caaagcaaac ttacagatag atttcatcca gtctgaactt
      181 aatttatcta ttagctgttt tgcttgttct ttggtgatcc agcgtacacg aattggcggc
      241 tctttctttt tcggtatgtg tgggactgca tccaacatac caagtttatg agcgatattt
      301 aatactctcg ataaagattt cacatactta ttttgcgtac tatgagatag aggctttttc
      361 gtatttgcat tctttttcgg gatatttagg ataatctcct gagctgttaa agagctgagt
      421 tcacgaccgg caaatttgga taaccagtat tcagcatgtc gcttttttgt ggctttatct
      481 tttaattctt cggccgcacg aacgtaatgg agcaaagcat cttcaaagat atattttctc
      541 ttttcctcga gtttatcctc tgcccacatt tcttgcttga gtttatcgtg atactgctga
      601 gcttcacgtt ttacaagcgt gcgagtgctt ctcttaattc tcttgccgtt tggggttgtg
      661 atatcaaccc accacggacc gtttgctttt cgtctgtaga tcgacatttt tcattctcct
      721 cgaccgacag agataaccct cggtcattat cagatcgttt tcttattggg tcaataccgg
      781 ctctcttttt tgctctttcc aaatcttctc ggttgatccg ccaaacttcc gagcctacca
      841 ttttaaaaaa tccccaatcc gccaaatgtt ggcggactgt atttggatgg caatttaatt
      901 cttttgccac ttgattaata gtaagatgtt ccattcatta ctttctccaa aataaatccc
      961 tctaaacgag ggattgtact aaataatcgt taagtctttg ccttatccac tgcatcaagg
     1021 cacagccata ccgtttccat ttggcacatt ggggaaacgg tgagctaata ccgcacaggg
     1081 gaatggctca atttcagaga accacaaagg cttacctaaa ccgtgccacg ctatacttgt
     1141 agcttcgata ccgctacaaa ttgagccgta ggtgaattgc attttttacc tatcctatcg
     1201 aaatactcaa attcccaata cggacaacat tttcttcgtt ggcttctagg atttctctca
     1261 acttagcata ggttttgggg catcttattt gacattcctc ttcgttgtag agagcctcta
     1321 atgtgtgcca ttcttttgca agtgcagccc aaactttccc aagttcagcc attttttcaa
     1381 tcctatttcg caaaaaaggg actgtctcca acaacagaaa gcaacgcctg aaatccgata
     1441 catcgtgtgg gtagccttct atttttggtc ttattccgta accaaggtaa aaagccattg
     1501 ttttactgct tacccctgtc tctccattgg caagccacca agcgattttg tcttctatgt
     1561 gcatttttcc tccaaaaatt aaccgcttgc ggaacaagtt ctacaagcgg ttgtcaagtg
     1621 ttcatttgtt caatgaacag ttcattgtga ataacaaaac cgcttgaatt acaagcggtc
     1681 tattttgttg cgtaaattta ctgttagcgt tgagccataa ccgactacga ccgctggccc
     1741 gaagatattg acggcgaggg ggacggcttc tcgcttgcct ccaaacggac gaccactttt
     1801 atgtcagccg gaatgacttt agtggagagt tcgcctggta aagatattgt cgatctcaaa
     1861 catatcccga aaagtagcca cgaagcccca ccgactaccg gtattttaag cctctacaat
     1921 cgaggcgacc gcagacgctt tagcgaaaca gttgataggt aagctatgaa aatccatacc
     1981 caaatccgaa aagaaatcct ttcattatta gaggcaaaat taacggacat cgagcatttt
     2041 tataacgggc aaccgaattt cattgatatt gacgaacagc aacttgccat ctctgtttat
     2101 cttgatgaga ttaaccgtca ggagcttacc ttatgcgatg aacagtggac agctcaactt
     2161 aacatcacga tttatctaaa atcagttgat gaggcagaag atgaattaga tgagtgggcc
     2221 gaaaaaatcc gtgaagtggt tgaaacatat tctgcgtttg aacatttaga gggcatcagc
     2281 ctttctcaat atcaatacga acaagatcaa aatcaaagaa cttggcactc agccacattg
     2341 atttttgatg ttgaatacta acaagcaaat aatccttata ggagaatcta caatggcaaa
     2401 aacaacaaaa gtccaaggca caaaatttag aattggcatt ggacgtgaag cccaaaaggc
     2461 aattacggct atcactttag ctacagcaac gcttactatt gccacatcag gctataaaaa
     2521 aggcgatgca atcgaaatta ccggttgtgg tcagttagac ggcatttatc ctgtattatc
     2581 tgtaacaggc gatcaagtca aattgtgcga agaagtaaac tggacgggta aagatttacc
     2641 ggctaattac accaaagcca aagcagcttt agtacaatac tcagaccaat tctgtgcggt
     2701 aaaaaacatt gaaaaatctg atgatacgtt aagtaccgaa gatgttacaa cggtctgctc
     2761 ggaaggtaca gaaaccgaac ccggagaaat tgaatttggc tcaattaaac tgagcttttt
     2821 tcacaagcca agtacggaaa tgcaaactcg cttacgcaaa ttgttttatg acaaatccac
     2881 ctttgcgtac aaattagaat tacctgataa ccacggcaca acctatggcg aaggtttcat
     2941 tgaggcgggt aacggcttta gtggcgaagt taaaggcaaa tatgaaggct cggtatcaat
     3001 taaaccaagc aaacgtgatt atttgctagt gtaattacaa aatattgctc caaatcgacc
     3061 gcttgtaata ttgcaagcgg tcttttttat cctaaatttt acaaaaggaa tcgacaatga
     3121 cactccgtga aaaactcctc gccaacaaac caaaattaca atctatcgaa atcaacggtg
     3181 agacctacta cctgcgtgaa gccactgttg gcgatatgaa taagcagatt tttgaaaccc
     3241 gaagctggct cattcaacaa gctgaacaag aaaatgttga attaccggca gaagatgatg
     3301 aaacctttga cgaagctctc aaccgttttg gcgaaaaata ccgccttgct caatcggttg
     3361 cctaccgttt atgtgacgaa aacggtgcat tactgtttaa cccacttaac attgacgatc
     3421 tcaatgcgat tgccgaatta gacagtaaag tgattatcga ctttaaccaa gccgtgtccg
     3481 ccccaaaaga ctcagcgagc gaagaaagtt ccaaataacc ctttcgctcg cactgggtaa
     3541 aacccttgaa gaaatcgaac aaatgcctga acgccacttt gccgaatatc agctttttta
     3601 tcaagagcaa ccttttgggc tttggcgtga ggactatcgt gctgctcaac ttgcccactt
     3661 aaccgcaatg atcaaccgtg acccaaaagg caaagcccct gaactctcgg aatttatgcc
     3721 gttttttaat cgggctgatg aagtagaagc aaatgaagat gatggcgttg cggattattt
     3781 ggcgaaaagg taaatctctt gtatttcatt gtaattctgt ctataattgg tgtaatacat
     3841 agaaatacag gagagataaa atggcgacta tcaatgatgc tttcagtttt agaacaaata
     3901 ccgaaataaa aaataccgca tttgatgtaa ttaaaaacta tggaatgaca ccctctcagg
     3961 tgtttaatat gtttttaacc gagattgcaa aaacgaaaac tattccgtta agtttgaatt
     4021 atcaacccaa tcttgaaaca aaattggcaa tgcaagaagc aaaatcaggt aaaaatgaag
     4081 tttatgcctc acttgaagca tttcataaag caatgttagc ggagtaaata atgctacaaa
     4141 tttcgccgac aaacgcatat aaaagagact ttaaaaagat tgcagccgaa ttagtcggta
     4201 gttcggaata tgtggaagta atgtattgcc taataaacca attaccattg gcggaaaaat
     4261 atagagatca cccactacaa ggtgaatggc aaggctttag agattgccat attaagcctg
     4321 atttagtgtt gatttacgct gttgaagata atctgctccg ccttgttcgt ttaggatcgc
     4381 acgctgaatt atttggataa tttctgtaaa aactaaccgc ttgcgatttt gtgctgtggg
     4441 cggtatagtt ggaatattaa caaaggaggg aaaaatgaaa aatgtacttc tatttatcgt
     4501 acactttttc ttaatcgcaa tgctctcaat tatcccaata ttaattattc ttggagcaat
     4561 cgaaagagat ccttactata ctgcgtggat tttccttagc gtatttttag gcattggagc
     4621 attgttatat tttgcaacca ttgtttctaa gttaattaac aagggaaaaa cacaaacatt
     4681 agaagaaaaa cgatatgcag aatattggaa taatgtaaaa gtcaatattc ccaaatagtc
     4741 taactgataa atgaccaaaa gctcgcttta tgcgggcttt tttattggag aaaaacaatg
     4801 agtggcttag gaaaattaac cgttacctta gagcttgaaa atgcaaaatt tcagtccgca
     4861 atgacgaaat ctgattatga ggcacaaaaa tttgccaaga actttatcca aaatatggat
     4921 agagctagaa atcacgccaa agaatttgcc gatcgctcaa cccaatattt aaaaaacatt
     4981 gagcaagcgg caaagaacct caataaaaat tctgaatttt catttttatc gactattggt
     5041 ggacattatc aatcaatgtc tagtggcata ttgagtgcgg caaaagccta tactgatatt
     5101 caaaataaaa tgaaattagt aagcggtagt tcagcagagg cagctaaacg attaactgat
     5161 gtgtttgata ttgcgactaa aacaagccaa agtacagagg ctgtttcagg tgtctatcaa
     5221 acatttgctc aaaatgccca atctctcgga ctggctcaaa aagatgtagc agaattaact
     5281 aaaacggtct ctcaagccgt tgctgcaagc ggcgcaagca gttctgcagc aagtaatgcc
     5341 ctaacccaat ttggtcaatc acttttaatg ggcaaaatga aagcccaaga atttaactcg
     5401 ctcattactc aaaccccaac cattattcaa gcaatggcaa aaggtttggg aatgacaatg
     5461 gcagaattta aagcagcggt ataccgggca aaatctttac ttggataaca ttcaagggca
     5521 aggttttgag agagtggata caccccaaat cggcgatgtg attttaatac aggtcggttc
     5581 tgatgtgccg aatcacgcag cgatttatat tggtgaacaa atggtaatcc accatagccc
     5641 gaaccgatta tctaagcgtg atttatatga cggttattgg ttacgccata cccacagtat
     5701 ttggcggcat aaactggcgg atagattgga ttttgacggc atatttaatg atataggaga
     5761 acaatatgaa atttagtaaa tatcaattat tagttaataa gatctgcttt atttgtggta
     5821 aaaaagattg tccacaaatg aaaaagagta aagactacaa agactttttg gatgctttag
     5881 aaaaagggga tgttgataaa gcagataaaa tatacaatac gaagttttca cagttttcta
     5941 aatcttttgg tcatgaaatg gaaaagaatt tagagaaaaa tcgtatacct cctatttatc
     6001 agggagttcc atttgattct tttgacttat atcagcatta ttcttatggt aatggagagg
     6061 cattaaatct ttcatccgtt ggattaagta ataaaattcg tcctctagtg aataaagata
     6121 aagcatttgg taaatccaat ggaagtatag aaagccgatt tatttcgcaa tataaaaatg
     6181 aaggacgcac atctttctcc aatgcttatg attttacaaa agaagcatcg ggtattgcag
     6241 atccgttatg ggcgttagga acagctacca ttagtggaga attgaaagat atacaaattt
     6301 ttcccggtgg tattgctagg ggaaatatcc attatagtat cagggacaga ttcactgacc
     6361 catttgatac attcaatttt actaaaggag aatggaaccc aaatggaaca ccttatatta
     6421 ttcaagatag ctggataaaa tcggtacgat tttcgataga caagaagata aaaataacac
     6481 cttaattatg a
//
LOCUS       DANLRK010000146         6460 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.146, whole genome shotgun sequence.
ACCESSION   DANLRK010000146 DANLRK010000000
VERSION     DANLRK010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6460
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.146"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            220..305
                     /locus_tag="PXA33_002072"
     tRNA            220..305
                     /locus_tag="PXA33_002072"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:254..256,aa:Leu,seq:caa)
     gene            399..1223
                     /locus_tag="PXA33_002073"
     CDS             399..1223
                     /locus_tag="PXA33_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDL5121375.1"
                     /translation="MKPLQNGTNKPFTITIASTKGGSAKSTNAANIGAFCAEHGLRTL
                     LIDTDTQPTLSSYYELEYQAPGGTYEFLHFKDVDPTHIISRTAIPNLDLIQSNDPSNK
                     ISPMLRDSPDGALRFSLLLQKIEGYDVIIIDTRGTRDITVDMSVLAADVLFCPILPHI
                     LSAKEFIRGTIGMYQDLETFTAFGFTLPPLKAMINCVDHTKDVKLVSDYLHTLFQNEF
                     DENKTLLEFSVPDRVAYREAATFSKPVYRQSQAEYETIRQLCALLMPQFAQSHFSE"
     gene            1235..2599
                     /gene="dnaB"
                     /locus_tag="PXA33_002074"
     CDS             1235..2599
                     /gene="dnaB"
                     /locus_tag="PXA33_002074"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HDL5121376.1"
                     /translation="MSNPNKPTTHLYSAEAEAAVIGGLLLDNTLFDDVIGKINSADFY
                     FGVHQALFKGITDLIEVGKPADILTLDEYLKQKNLLKEIGGFAYLAEVSKNTPSAANV
                     GAYADVVLLHSKHRQLLKLGQFIVDQTQTVKTPEKLESVIDEVEKKMTEFSLSDNTKS
                     ATDLSDIFASMLSRMELSAKNGDPVTGTPTGIQGIDEATTGGQPGDLIVIGARPSMGK
                     TAFSQTIAYHTLEKFESAPVFYHSMEMPADQILQRFLAMRARVGLQNIRQADRLDDEE
                     WAKLTAAMGYILEHWKNRLVIDDEGSLTPQKLRTKVRQNARKYGKPSAIFIDYIQLMQ
                     TSQRYENRNLEIANISRSLKLLAKEMDCPVYALSQLNRSLEQRANKRPINSDLRESGS
                     LEQDADVILFIYRDEIYDEDSAFKGTAEIIIGKQRNGPLTSVMTNFRSEFALFENRAS
                     TASH"
     gene            2608..4257
                     /locus_tag="PXA33_002075"
     CDS             2608..4257
                     /locus_tag="PXA33_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HDL5121377.1"
                     /translation="MTKDEKRLAAIAKGLNVSPISNTAPSYQTATPAHYSSDEEYITV
                     TLDKLRPYEHNPRKTRNPNFEMIKESIRRRGLDHKPNITRRPGEDFYIIADGGNTRIQ
                     ALKELFTETKDPKFWSISCQYKPWQGDTADSVEAELNLLIGHLIENDTRADLSFIEKA
                     LGILQAKEYYEKKLNKSLSARELATELEGDGYIIHYTLANKMERCVLYLYPHIPEVLF
                     KGLGHTQIDKLLAIRNNAEEVWAAHQFDTDITFESLWSENLSKFNEATPFQAKEFQSE
                     LITAMVEALDGKVSFETLYLDIDLDEQKFKKIAAKQQEIDNLVEKTVDQINTRQEQQA
                     AKFEPVIKNENKSVATESAKNVIDDVIDDNESDNEQNNAITDLSQLDAPLLDSQDNQT
                     EDYLSQIAHNFGLTPGMSIQEQREQRAESNGLTFACEGRQPVEDIWLIHPARQYRSEA
                     YSLALDIAESVGIDHLVEHVVKQPVDYTYAMKPTDSELSPSVLTLYSFLSMLQTAKHK
                     DENNCVLDSNFLFELDDITLVKLFRLIRVSRHIHQQGGEND"
     gene            4250..4807
                     /locus_tag="PXA33_002076"
     CDS             4250..4807
                     /locus_tag="PXA33_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2857 domain-containing protein"
                     /protein_id="HDL5121378.1"
                     /translation="MIKPITVNEALIGNALLNIKEGNIHPCLKMGFSEEQLKAINNLS
                     IDEIMDISNSVVSFAKVEINHETFWKLLAIAQTNTQQRQIIDRALLLGASIEMLHQYF
                     GLSTSEVSARRQLLGIEEKMGRKAAASDEESTHIWEIWQRYKQRMESLDSQEGLELLC
                     LIAEEGNMNLTVVWKLVSEWYSKHK"
     gene            4966..6151
                     /locus_tag="PXA33_002077"
                     /pseudo
     CDS             4966..6151
                     /locus_tag="PXA33_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252650.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2119 a         1247 c         1305 g         1789 t
ORIGIN      
        1 acaggtgatg tgcataacta tttttgtaaa gatgaattga gtgctgattt ttaaatcaac
       61 cgatttcaaa aggcattttt acgcttgcac aagcggtcat atttccctat aatcgtgcaa
      121 aaattaagcc agtgtggcga aatcggtaga cgcagcggat tcaaaatctt ttgcgtagac
      181 taataggaaa tactggcgat tgaataaaaa acactctcag cctgagtgat gaaattggta
      241 gacatgatgg attcaaaatc cattggtgaa taaccgtgtc ggttcgagtc cgacctcagg
      301 caccattcaa ctgcgttcct ttcaaataat actcactcta aatatacagt ttataatgcc
      361 aatactcctt aacttcaact ttgttaagag agtaaattat gaaaccatta caaaacggta
      421 caaacaaacc ttttaccatt actattgctt cgaccaaagg aggctctgcc aagagtacga
      481 atgccgctaa cattggtgca ttctgtgcag aacacggtct aagaaccctt cttattgata
      541 ccgatactca acccacatta agctcttatt atgagttgga atatcaagct cccggtggaa
      601 cttatgaatt tctgcatttt aaagatgtag atcctaccca cattatttct agaacggcta
      661 tcccaaatct tgacttgatc cagtctaatg acccgtcaaa taaaatcagc ccaatgctaa
      721 gagactcacc ggacggggct ttacgtttta gtttactgct ccagaaaatt gaaggctatg
      781 atgtcattat cattgatacc cgtggcactc gagatattac tgtagatatg tcggtattgg
      841 ctgctgatgt tcttttctgc ccgattcttc ctcatatctt gtcagctaaa gaatttattc
      901 gtggcacaat tggtatgtat caagatctag aaacttttac agcctttggt tttaccctgc
      961 caccgttaaa agcaatgatt aactgtgttg atcataccaa agatgtcaaa ttggtttccg
     1021 actacctgca tactctcttc caaaatgagt ttgatgaaaa caaaacatta ctggaatttt
     1081 ctgtgcctga tcgtgtggca taccgagaag cggcaacttt ttcaaaacct gtctatcgcc
     1141 agagccaagc tgaatatgaa accattcgtc agctttgtgc attactgatg ccacagtttg
     1201 cacaatcaca tttttctgag tagttggggg atttatgtct aacccaaata aaccaaccac
     1261 ccatctctat tcagcagaag ctgaagccgc tgtcatcggg ggattgttat tagataacac
     1321 tctctttgac gatgtcattg gcaagattaa ttcggctgac ttttattttg gtgtgcatca
     1381 agccttattc aaggggatta cggatttaat tgaggtgggg aaacctgcag atattctcac
     1441 attagatgaa tacctaaaac agaaaaatct gttgaaggaa atcggcggtt ttgcctatct
     1501 tgcagaagtt tcaaaaaaca ccccttcggc ggccaatgtg ggggcttatg ctgatgtggt
     1561 tctgttacac agtaaacacc ggcaactttt aaaactgggg cagtttattg tcgatcagac
     1621 acagactgtg aaaacaccgg aaaaacttga atcagttatt gatgaagttg agaaaaaaat
     1681 gactgaattc agcctttctg acaataccaa atctgcaaca gatttaagtg atatttttgc
     1741 cagtatgctc tctcgtatgg agctttcggc taaaaatggt gatcctgtga ctggaacacc
     1801 gacaggtatt caaggcatcg atgaagcgac taccggtggg caaccgggtg atttaattgt
     1861 gattggtgcc cgtccttcga tgggaaaaac cgcattttct caaactattg cctaccatac
     1921 acttgaaaaa ttcgagtcgg ctcctgtgtt ctatcacagt atggaaatgc cggcagatca
     1981 gattttacaa cgttttcttg ctatgcgtgc ccgagtcgga ttgcaaaata ttcgacaagc
     2041 agaccgcttg gacgatgagg aatgggcaaa attgacagct gcaatggggt atatccttga
     2101 acactggaaa aatcgcttag ttattgatga tgaaggtagt ctgactcctc aaaaattaag
     2161 aactaaggtt cgccaaaatg cccgtaagta tggaaaaccg tcagccattt ttattgacta
     2221 tattcagctt atgcagactt cgcaacgcta tgaaaaccgc aatttggaga ttgcgaatat
     2281 ttctcgctca cttaagttgt tagccaaaga gatggattgc cctgtttatg ctttatctca
     2341 gctgaaccgc tcattggaac agcgggccaa taaacgccct atcaactctg atttacgaga
     2401 atccggctca cttgagcaag atgccgatgt cattttattc atctatcgag atgaaattta
     2461 tgatgaagat tccgccttca aaggcactgc tgaaattatt atcggtaaac aacgaaacgg
     2521 accgctgact tcagtaatga ccaattttag aagtgagttt gcactttttg agaatagagc
     2581 aagcacagca tcacattagg acagattatg acaaaagatg aaaaaagatt agcagctata
     2641 gcgaaggggc taaatgtttc acctatttca aatacggcac cctcctatca gactgcgaca
     2701 ccggctcatt acagtagtga tgaggaatat attacggtga cattagataa gttacggccg
     2761 tatgaacata accctagaaa aacccgtaat cctaactttg agatgatcaa agagtcaatc
     2821 cggcgtcgtg ggttagatca taaacctaat attacccgcc gtccgggaga agatttttac
     2881 attatcgctg acggcggtaa tactcgtatt caggcactta aagaattgtt tactgagacc
     2941 aaagatccga aattctggtc tatcagttgc caatataagc cttggcaagg tgatactgcc
     3001 gacagtgtcg aagccgaact caatttactt atcgggcatt tgatcgaaaa tgatacccgt
     3061 gcggatttgt catttattga aaaagctctt ggtattttac aggcgaaaga atattacgag
     3121 aagaaattga ataaatcact ttcagcaagg gaattagcta ctgagttaga gggtgatggc
     3181 tatattatcc actatacgtt ggctaataaa atggagcgat gtgtacttta tctatatccc
     3241 catattccag aagttctatt taaaggttta gggcatactc aaatcgataa gttattagct
     3301 attcgtaata atgccgaaga ggtatgggca gcccatcaat ttgatactga tattactttt
     3361 gaatcgctct ggtcagaaaa tttatccaaa tttaatgaag ctacaccatt tcaagcaaaa
     3421 gaattccaaa gtgaattgat tacggcaatg gttgaagctt tagatggaaa agtgagtttt
     3481 gaaacacttt atctagatat tgacttagat gaacaaaaat tcaaaaaaat agcggctaaa
     3541 caacaagaaa ttgataattt agttgaaaaa acagtcgatc aaatcaatac cagacaggaa
     3601 caacaagcag caaaatttga accagttatc aaaaatgaaa ataaatctgt tgccacagaa
     3661 tcagctaaaa atgttattga tgatgtaata gatgataacg aaagtgacaa tgagcagaat
     3721 aacgccatta ctgacttatc tcagctagat gctccattgt tagatagcca agataatcaa
     3781 actgaggatt atctctccca aattgctcat aacttcggac taacaccggg aatgagcatt
     3841 caagaacagc gagagcaacg agcagaatcc aatgggctca ctttcgcttg cgaaggtcgc
     3901 cagccggttg aagatatttg gttgattcat cctgctcgtc aatatcgttc agaggcgtat
     3961 tcccttgcct tagatattgc agagagtgtg ggcattgacc acttggttga acatgttgtg
     4021 aaacagcctg tggattacac ttatgcaatg aaaccgacag atagtgaact ttcaccgtct
     4081 gttctaacac tctattcatt cctttcaatg ttacagacag ctaaacataa ggatgaaaat
     4141 aactgtgtcc tagattccaa cttcctgttt gagcttgatg atattacctt agtcaaactt
     4201 ttccgtctca ttcgggtcag tcgccatatt catcaacaag gaggagaaaa tgattaagcc
     4261 aattaccgta aatgaagcat tgattggtaa cgccctgctc aatattaaag aaggaaatat
     4321 ccatccttgt ttaaaaatgg gattttctga agagcaacta aaagcaatca ataatctcag
     4381 tattgatgaa attatggata ttagcaactc cgtagtgtct tttgcaaaag tagagattaa
     4441 ccacgagact ttttggaaat tactggcaat tgcacaaact aatactcaac agcgacaaat
     4501 tatagaccgg gctttattac tgggagcctc tattgagatg ctgcaccagt atttcggttt
     4561 atcgacatca gaggtttcag cacgccgtca attactcggc attgaagaga agatgggacg
     4621 gaaagccgca gcaagtgatg aggaatccac tcatatttgg gagatttggc aacgatataa
     4681 acaacgaatg gaaagcctag attcacaaga agggttagag cttctttgcc tgattgctga
     4741 ggaaggtaat atgaatctaa ctgttgtttg gaaactagtt tccgaatggt attcaaaaca
     4801 taagtaaaaa acaaatgccc cgaagttgga gctttggggc attcgatatt gggtcttgta
     4861 tatctatcct atgaattaca aggactttgc cgaagtcccg tgcattatag tcataggagg
     4921 gagaatagca agagaaaaac tcttttatcc tagtggagag aaacaatgtt tagcagtacg
     4981 aataagccaa cagggcatgg attactattt tttggcaacc aacacgaaac agtccctact
     5041 cgattattac aagatccctg ccttacacca agagcaaagt ttgcttggca gctcattaag
     5101 tgcaatgttc aggcctttca aggaggatta tttccctctt atgagacgct cagcaaaatg
     5161 ttatccgata gaccttatgc ggaggctaaa ttatcggaaa aagtcgtgac tcaaacactc
     5221 tatttactcc gtcttactcg ctggcttact ttatgtgaaa cagtgcgtga tgagcgaggt
     5281 tatgtacaag gtaatgtgta tatcctgcac gatgaacctg tgccaatttt agatgcaatt
     5341 caattaaata gcgactatct ctctttttta gataaatgca ccaaacataa agatagcctt
     5401 attaatggtg tggcaaatca tattgttgaa agtctaatga cagacaaaac caaatggcat
     5461 tatgtctctc atttagactg tttgcaaagt cgttatcaaa cataccagca acagcagctg
     5521 gtgaaagata aacagccatc gcaaacagaa caagaattaa gcaaaattca acaaaatatc
     5581 ctgacttcca ctatggaagt tagggatttt gatgaggaag tcaggaaaaa atcctcaaaa
     5641 aaccagactt ccaataagga agttagggct aaaaatgaag ataagtcatt gattttagac
     5701 ttacttccat taaggaagtc agatgtacag tactttatat attaataagt actgtactgg
     5761 ggaacctagc gaaattactt ggccacaaga aatcccacta acctcacttg aacaaaaact
     5821 cactgcacag gcaatgaaag gattcgatct cggtttatgc caagctattt tgctggaagc
     5881 acaacaacgc attattcaaa agcaggatgt gaaaaaaccg aaaggttatc tctatacgct
     5941 tatccagcgg gcgaagcagg gtgaatttaa accctactat tttgaattag gaaaaaatct
     6001 tccctcacaa aatcagacta acaataattc tgctcattct atacaaaaac tgactgagca
     6061 gcctactgag aaaccggtat taactccagc agacagagag caaatggcga gccgtatgca
     6121 gaagtttaga gctgaattac ttttacaata aggttaaaaa acaatcactg ttaaggggct
     6181 taacagcgag caaaaaacag acagcgtgta gggattacac agtgagtgaa aaataatcag
     6241 tgaagcggcc cgcttcaaag gctaaaaaat aatcactgta aacactgttt acaatgatta
     6301 aaaaacaatc agtgtagagg ggttctacag tgagtaaaaa ataatcagtg tagagggact
     6361 ctacaatgag caaaaaacaa tcagtgtgta gggggtacac agtgattaaa aaacaatcag
     6421 tgtagagggg ttctacagtg agtaaaaaat aatcagtgta
//
LOCUS       DANLRK010000147         6447 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.147, whole genome shotgun sequence.
ACCESSION   DANLRK010000147 DANLRK010000000
VERSION     DANLRK010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6447
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.147"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            469..2001
                     /locus_tag="PXA33_002078"
     CDS             469..2001
                     /locus_tag="PXA33_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HDL5121379.1"
                     /translation="MNLVDYSTSLWSVLPALIALILAIVTRKVIISLSIGILFGAFML
                     TNSNFLDALTYIKNSVVSLVYKGAEEGLNSNNVNIILFLLLLGVLTSLLSISGSNQAF
                     ANWAQKKVKGKRGAKLMAAGLVFFTFIDDYFHSLAVGAIARPVTDKFRVSRAKLAYIL
                     DSTAAPMCVLMPISSWGAYIITLIAGLLATHSITGYSPLGAFMTMSAMNFYAIFAMIM
                     VFFVAYFSFDIGSMARFERQAAHLAELDDEQIETKGHVRDLILPIIALIVATVSMMMK
                     TGADALAESGASFSILGAFENTTVGISLVVGGVAGLIVATICIITSGNISFKNYVRAY
                     GLGVKMMSGAITILFFAWTINGVVSDMQTGKYLSGLVAESIGAEFLPAILFVLAAAMA
                     FSTGTSWGTFGIMLPIGASMAVHSDPALIIPCLSAVMAGAVCGDHCSPVSDTTILSST
                     GAQCNHMDHVISQLPYALLVATASIIGYLVVGFTQSAVLGFIATAVVMAILILLFKEK
                     KH"
     gene            2227..2979
                     /locus_tag="PXA33_002079"
     CDS             2227..2979
                     /locus_tag="PXA33_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL5121380.1"
                     /translation="MKIALVTGVTAGFGLAICKLLLQSGYKVIGTGRRAERLEELHLQ
                     FGDKFLPLAFDIQQPDEVEKALNSRPKAWQQIDLLVNNAGLALGLEPAQKADLNDWYQ
                     MIDTNIKGLVTMTRLVLPEMVERKSGHIINIGSIAGTYPYPGGNVYGGTKAFVKQFSL
                     NLRADLAGTKVRVSNVEPGLCGGTEFSNVRFKGDDEKAAKVYENVQFVRPEDVANIVL
                     WLNQQPEHVNINRVEVMPVAQTSSALNVARNI"
     gene            2991..4181
                     /gene="trpB"
                     /locus_tag="PXA33_002080"
     CDS             2991..4181
                     /gene="trpB"
                     /locus_tag="PXA33_002080"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HDL5121381.1"
                     /translation="MSDTLLNPYFGEFGGMYVPEILVPVLKELEQAFVDAQQDPTFQA
                     EFADLLKNYAGRPTALTLCRNLTKGSKTKLYLKREDLLHGGAHKTNQVLGQILLAKRM
                     GKTRIIAETGAGQHGVATALACAMLGMPCVVYMGAKDVERQSPNVFRMRLMGAEVIPV
                     QKGSCSLKDACCEAMRDWAANYDTTHYLIGTAAGPHPFPTMVREFQKMIGEEIKRQIL
                     AKEDRLPDAVIAAVGGGSNAIGMFADFIEEQSVKLIGVEPAGKGIETGEHGAPLKHGT
                     TGIYFGMKSPIMQDKDGQIEESYSISAGLDFPSVGPQHAYLNATGRAEYVSITDAEAL
                     EAFQALAKNEGIIPALESSHALAYALKMIKQNPEKEQLLVVNLSGRGDKDIFTVDKIL
                     NGGE"
     gene            4183..4989
                     /gene="trpA"
                     /locus_tag="PXA33_002081"
     CDS             4183..4989
                     /gene="trpA"
                     /locus_tag="PXA33_002081"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HDL5121382.1"
                     /translation="MSRFDTLFTALKAKNEGAFVPFVTLCDPGFERSFEIIDTMIKNG
                     ADALELGFPFSDPLLDGPVIQAANKRALDGGYSTDSCFELIAKIRAKYPDIPIGLLLC
                     ANLIFVPTAEVFFQRCQQSGVDAVLIADLPVFAAQEFVPTAEKYGIQPVFICPPNADQ
                     NTIERIAQLTQGYTYLVSRAGVTSAENQAHASNLDNLVESLKQAGAAPILQGFGIAKP
                     EQVKEALRLGADGAISGSAIVKIIERNLADQANLLKELAEFVQVMKAATK"
     gene            5039..6445
                     /locus_tag="PXA33_002082"
     CDS             5039..6445
                     /locus_tag="PXA33_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HDL5121383.1"
                     /translation="MKLTKIVLSVVAVIGAVAVGGSWYTGKQVEEKYQELVVQANNQL
                     KQLSGEFGANIELKDVQIDRHFFISNAKYRLEVDLGNGKKLDFVGNDKLHHGPLPLNR
                     LAKLNLAPVMMSAESKLQAPEALKKLMGEHIGTGFSNISYSGDVEGHFDISPIKHSDE
                     TGHIETTPIKVQYSYDQKLKGVDSTVELESLKIKAEEFDFQIQGIVYDVQTENNESYP
                     NLTLGKGSGKINAIEFKSKDGKLSQIKDVVVKGDNLLKGDRLISTGDLEAATFNIEGV
                     EMGKLKMDMAYDLDAKLTNDITPLLSNPQTLENEKTGELLLQLLAKSFKFHINNFSLE
                     NSKGKVDLALLLNMAQFDPQNLGNMQAVLQALSTSKFTSNINRQYAEDIIRQVSIVTE
                     KLGEEEAKAFAKQQVDAVFLNAGVEQYGLRKVDDNNVKIELTIDNGKVNLNGRELTED
                     ELQMALFMIVMGAGSLGQ"
BASE COUNT         1940 a         1108 c         1439 g         1960 t
ORIGIN      
        1 cggatattca aaagactttt taaagctgac attttactct cctaaattaa tgttttatat
       61 aagtagcttt tatgctcctt ttatcatatc tcaaaaggca ttttaggtaa ataagcataa
      121 ataaaaaaat agatacttta tatctaaaga tattcgtgct atcttaaaca ctgtcctaca
      181 aattgtagag gtgcgaattc aataagtatt tcttctgagt ggaaaacgat gaaggggaag
      241 gaaaggtgaa tttgccgaaa tcaattaagc gtcatcttaa ttggttgggg tcgttgtcga
      301 aagaaacgac actgtcgtag taaatgctac ggagtgctac tgttagggtg ggtttcagat
      361 atttcacatt tgccttcctt tcatatttat ttttataaat atcccagtag atctcttgtt
      421 tggctattct tatttataca aattatacac agtcatttta gaggttttat gaatctagtt
      481 gattattcta cgtcactttg gtcggtattg ccggcactga ttgcgctaat attggcaatt
      541 gtgacacgca aggtgattat ttcacttagc atcggtattc tctttggtgc atttatgctt
      601 actaatagca attttcttga tgcacttact tatattaaaa atagcgttgt ttctttagtt
      661 tataaagggg ctgaagaagg attaaattca aataatgtta acattatttt atttttgctt
      721 ttattagggg tattaacgtc attattaagt atttcaggta gtaatcaggc gtttgcgaat
      781 tgggcgcaga aaaaggtaaa aggcaaacga ggcgcaaaat taatggcggc aggtttggtt
      841 ttctttacct ttattgatga ctatttccac agtttagcag taggtgcgat tgcccgccct
      901 gttacggata aattccgtgt ttctcgtgct aaattagctt atattttaga ttctaccgcc
      961 gctccaatgt gcgtgttaat gccaatttca agctgggggg cttatattat taccttaata
     1021 gctggcttat tggctacgca ttccatcaca ggctattcgc cactaggcgc atttatgaca
     1081 atgagcgcaa tgaactttta cgccattttt gcgatgatta tggtgttctt tgtggcatat
     1141 ttctcatttg atattggttc gatggcgcgt tttgaacgtc aagcagcgca tttagcagaa
     1201 ttagatgatg agcaaattga aacgaaagga cacgtgcgcg atttgatttt gccaattatt
     1261 gcacttattg ttgctacagt ctcaatgatg atgaaaacag gtgcagatgc actggccgaa
     1321 tcaggtgctt cattttctat actaggtgct tttgaaaata ctacggtagg catttcatta
     1381 gttgttggtg gtgtggcagg gcttatagtt gcaactattt gtatcattac aagcggtaat
     1441 atttctttta aaaattacgt ccgtgcttat ggcttaggtg tgaagatgat gtctggtgca
     1501 atcactattc tcttctttgc gtggacaata aacggcgtag taagtgatat gcaaacaggg
     1561 aaatatctat caggattggt tgcagaaagc attggagcag agtttttacc tgcaattcta
     1621 tttgtgttag ccgcagcaat ggcgttttca acaggaacta gctggggaac atttggtatt
     1681 atgttgccaa ttggagcctc aatggcagtg cattcagatc cagcattaat tattccttgt
     1741 ttatctgcag taatggctgg tgcggtttgt ggtgatcact gttctccagt atctgatacc
     1801 actattcttt cttcaacagg cgcacaatgt aatcatatgg atcatgtcat ttctcaatta
     1861 ccttatgctt tattagttgc aacagcttct attattggtt acttagtggt tggctttacc
     1921 caatcagcgg tattgggatt tattgctaca gctgttgtta tggcaatact cattttgctt
     1981 tttaaagaaa aaaagcatta atttccgaaa atgtgaaggg aatcacagaa tgagatttat
     2041 tgatgagata aaatagcact caagatatgg atatcgaatg ttgtgttata taaatcttaa
     2101 aataagatcc tcctagcatt agcgtacctt ttggtacgct tttttctttt atggcaatat
     2161 tatttcttgc aattttaatt tttttgtact actttaatag tacattttaa atttgagggt
     2221 gaaaagatga aaatagcatt agtcacagga gtaactgctg gatttgggtt ggcaatttgt
     2281 aaattattgc ttcaatcggg ttataaagta attggcacgg gcagacgggc agagcgttta
     2341 gaagaactgc atttacaatt tggcgataaa tttctaccgc ttgcatttga tattcaacag
     2401 cccgatgagg tggaaaaagc actgaatagc cgccctaaag cgtggcaaca aattgattta
     2461 ttagtaaaca atgcaggcct tgcactcggc ttagaaccgg cacagaaagc agatttgaac
     2521 gattggtatc aaatgatcga taccaatatt aaaggattgg taacgatgac acgcttagtt
     2581 ttgcctgaaa tggtggagcg gaaaagtggg catattatta atatcggctc gattgccggc
     2641 acttaccctt acccgggcgg taatgtatat ggtggtacta aagccttcgt gaaacagttt
     2701 agcctaaatt tgcgggcaga tttagccggc actaaagtgc gtgtttccaa tgttgagccg
     2761 gggctatgtg gcggtacgga attttcaaat gtacgcttta aaggcgatga tgaaaaagcg
     2821 gctaaagttt atgaaaacgt gcagtttgtg cgcccggaag atgtggcaaa tattgtgctt
     2881 tggctgaatc agcaacctga acacgttaat attaatcggg ttgaagtgat gccggtggct
     2941 caaacttctt ctgccttaaa tgtagcgaga aatatttaaa aggaaatatt atgtcagaca
     3001 cacttcttaa tccttatttt ggtgaatttg gtggtatgta tgtgccggaa attttagtgc
     3061 cggtgctaaa agaattagaa caagcctttg tagatgctca gcaagaccca acattccaag
     3121 ctgaatttgc cgatttactc aaaaactatg ccggtcgccc gactgcttta accctttgtc
     3181 gtaatttaac caaaggcagt aaaaccaaac tctatttaaa acgtgaagat cttttacacg
     3241 gtggggcaca taaaaccaac caagtgctag ggcaaatctt actcgctaaa cgaatgggta
     3301 aaacccgcat tattgccgaa acaggagcag gacagcacgg tgtggcaacc gcattagcgt
     3361 gtgcgatgtt aggaatgcct tgcgtggtgt atatgggcgc aaaagatgta gaacgccaat
     3421 cgccaaatgt attccgtatg cgtttaatgg gagcggaggt gattccggta caaaaaggct
     3481 cttgctcgct caaagatgcc tgttgcgagg caatgcggga ttgggcggca aattatgata
     3541 ctacccatta cctaatcggc actgcagccg gcccgcaccc gttcccgaca atggtgcgtg
     3601 aatttcagaa aatgattggc gaagagatca aacgtcaaat tttagcaaaa gaagaccgct
     3661 tgcctgatgc ggtgattgct gccgtaggcg gtggctcaaa tgctatcggt atgtttgcag
     3721 attttatcga agagcaatcc gtcaaattga tcggtgttga gccggcagga aaaggaattg
     3781 aaaccggtga acacggtgca ccgctcaaac acggcacaac ggggatctat ttcggtatga
     3841 aatcgccgat tatgcaggat aaagacgggc aaattgaaga gtcttactcc atttcagccg
     3901 gcttagattt cccttctgtc gggccgcaac acgcttattt aaatgcgaca gggcgagctg
     3961 aatatgtatc gattaccgat gcagaggctt tagaggcatt ccaagcatta gccaaaaatg
     4021 aggggattat tccggcgtta gaatcttccc acgccttagc ctatgcgtta aaaatgatta
     4081 agcagaaccc tgaaaaagag cagttattag tggtgaattt atcgggtcga ggcgataaag
     4141 acattttcac cgtggataaa attttaaatg gaggagagta aaatgagccg ctttgatacg
     4201 ctatttactg cattaaaagc aaaaaacgaa ggggcttttg tgccgtttgt gacattatgc
     4261 gatcctggtt ttgaacgcag ttttgagatt atcgacacga tgattaaaaa cggggctgat
     4321 gctttagagc taggctttcc gttttctgac cccctcttag acgggccggt gattcaagca
     4381 gcgaataaac gagctttaga tggtggctat agcactgata gctgttttga gctgattgcc
     4441 aaaattcgag ctaaatatcc agacattcct atcggtttac tactgtgtgc caatttaatt
     4501 tttgtgccaa ccgcagaagt atttttccaa cgttgccaac aaagtggagt tgatgcggtg
     4561 ttgattgctg atttgcctgt tttcgctgct caagagtttg tgccaaccgc tgaaaaatat
     4621 ggaattcagc cggtgtttat ttgcccgccg aatgctgatc aaaacaccat tgaacggatt
     4681 gctcaattaa cccaaggcta cacttattta gtttcccgtg ccggtgtaac tagtgctgaa
     4741 aaccaagctc acgcaagtaa tttggataat ttagtggaaa gtttaaaaca agccggagct
     4801 gcccctatct tacaaggttt tggcatagcc aaacctgaac aggtaaaaga ggcgttaagg
     4861 cttggggcag acggggcgat ttcaggctca gcgattgtga aaattattga acgtaattta
     4921 gctgaccaag ctaatttgct caaagaactt gccgaatttg tacaagtgat gaaagcggct
     4981 acgaagtaaa ctacaagcgg tcatttttta aatttttttg aaaataaaag gaaacagaat
     5041 gaaattaaca aaaattgttc tttcagttgt agcggtaatt ggtgctgttg cagttggcgg
     5101 aagttggtac acaggtaagc aggttgaaga aaaatatcaa gaattggttg ttcaagctaa
     5161 taaccaactt aaacaactga gtggcgaatt tggtgcgaat attgagctta aagatgtgca
     5221 gatcgatcgc catttcttta tctctaatgc gaaatatcgt ttagaagtgg atttgggtaa
     5281 tggtaagaaa ttggactttg ttggtaatga taaacttcat cacggtccgc taccgcttaa
     5341 tcgtttagcg aaacttaatt tagctcctgt aatgatgagt gcggaaagca aattacaagc
     5401 tcctgaagcc cttaaaaaat tgatgggtga acatattggc accggttttt ctaatatcag
     5461 ctattcgggc gatgtggaag ggcattttga tatttccccg attaaacata gtgatgaaac
     5521 aggtcatatt gaaaccacgc caatcaaagt gcaatatagc tacgaccaaa aattaaaagg
     5581 ggttgatagc actgttgagc tagaaagtct taagataaaa gcagaagaat ttgattttca
     5641 aattcaaggt atagtttatg atgttcaaac agaaaataat gaaagttatc ctaatttaac
     5701 tttaggcaaa ggtagtggaa aaattaacgc tattgagttt aagtctaaag atggcaaatt
     5761 atctcaaatt aaagatgttg tggtgaaggg cgataatcta ttaaaaggcg atcgtttaat
     5821 ttctaccgga gatttagagg cagccacttt caacattgag ggtgttgaaa tgggtaaatt
     5881 aaaaatggat atggcatacg atttagatgc taaattaacc aatgatatta cgcctttatt
     5941 atctaatcct caaacattag aaaatgaaaa aacaggcgag ttattattac aactattagc
     6001 aaaatcattt aaattccata ttaataattt ctcacttgaa aacagcaaag gcaaagttga
     6061 tttggcttta ttactgaata tggctcaatt tgacccacaa aatcttggta atatgcaagc
     6121 ggtattgcaa gccctttcaa caagtaaatt cacttcaaat atcaaccgtc aatatgcgga
     6181 agatattatt cgccaagtct caatagttac agaaaaatta ggcgaagaag aggcgaaagc
     6241 atttgccaag caacaagttg atgctgtttt tctcaatgcg ggagttgaac agtacggatt
     6301 aagaaaagta gatgacaaca atgtgaagat tgaattaact atagataacg gaaaagtgaa
     6361 tttaaacgga cgtgaactga ccgaagatga acttcaaatg gctctgttta tgattgtgat
     6421 gggagcaggc tcactcgggc aataatc
//
LOCUS       DANLRK010000148         6438 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.148, whole genome shotgun sequence.
ACCESSION   DANLRK010000148 DANLRK010000000
VERSION     DANLRK010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6438
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.148"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            49..2706
                     /gene="aceE"
                     /locus_tag="PXA33_002083"
     CDS             49..2706
                     /gene="aceE"
                     /locus_tag="PXA33_002083"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005628834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HDL5121384.1"
                     /translation="MSENLRNDVDPIETQDWLASLDSLIREEGLERAQFIIEQVISQA
                     RAGGVALPTGVTTDYVNTIPVSEQPAYPGDHKIERRIRSAVRWNAIAAVLRSQKKDLD
                     LGGHISTFQSAATMYEVCFNHFFKAPTEKDGGDLVFFQGHAAPGMYGRAFLEGRITAE
                     QMDNFRQEAFTDGLSSYPHPKLMPEFWQFSTVSMGLGPVNAIYQARFLKYLENRGLKD
                     TSAQKVYAFLGDGEMDEIESKGALSFAGREKLNNLIFTISCNLQRLDGPVNGNGKIVQ
                     ELEALFTGCGWEVIKVLWGSGWDKLLEKDTSGKLKQLMMEVLDGDYLTFKSKNGAYVR
                     EHFFGRYPETAALVADMTDDEIWALRRGAHDSEKLYAAYAKAQKSDKPVVILAHQVKG
                     YRIPEAESKNTAHQSKKMSLDSLKGFRDYFELPLTDEQVESLEYVRFPEGSEEYNYLH
                     GQRKALNGYVPVRKPKFTVDFKVPELSEFQALLDEQPRGISTTMAFSRVLNTLLKDKN
                     IGQQIVPIIADEARTFGMEGLFRQIGIYNPHGQNYVPSDRDLVAYYREAKDGQVLQEG
                     INELGATASWVAAATSYSVSNLPMIPFFIYYSMFGFQRVGDMMWLAGDQLARGFMIGG
                     TSGRTTLNGEGLQHEDGHSHIQAGVIPNCVTYDPAFAFEVAVIVQDGIRRMYGENQED
                     IFYYITTLNEITEQPAMPAGAEEGIRKGLYKFETVEGKGKGHVQLLSSGAIMRHVRAA
                     AQILANDYGITADVFSAPSFNELGRDGADVARWNLLHPTETPRVPYVAQVLQDLPTVA
                     STDYMKMYAEQIRAYVPSKHYHVLGTDGFGRSDSRANLREHFEVDERYVVVAALAQLA
                     KEGTVENKVVAEAITKFGLNVDRINPLYA"
     gene            2910..4820
                     /gene="aceF"
                     /locus_tag="PXA33_002084"
     CDS             2910..4820
                     /gene="aceF"
                     /locus_tag="PXA33_002084"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HDL5121385.1"
                     /translation="MSKQINVPDIGSDEVSVTEVMVKVGDTISVDQSIINVEGDKASM
                     EVPAPEAGVVKEVLVKVGDKVSTGSPMLILESADAAPAQEAPKAEAAPAAPVATTSAV
                     VEVNVPDIGGDEVNVTEIMVKVGDSVEVDQSIINVEGDKASMEVPAPVAGVVKEILIN
                     VGDKVSTGKLIMKFETASAATAQSTPVAEAAPAQASASAIKDVNVPDIGGDEVNVTEI
                     MVKVGDSVSEEQSLITVEGDKASMEVPAPFAGVVKEILVKSGDKVSTGSLIMKFEVAG
                     AAPAPAAAQAASAPAPAPQAAAPAKADAAPAGQNQSGLSQEQVVASAGYAHATPVIRR
                     LAREFGVNLDKVKGTGRKGRIVKEDIQAYVKTAVQVFEKQGGTAAAATGAANGAGLGL
                     LPWPKVDFSKFGEVEEVELSRINKISGANLHRNWVMIPHVTHFDRTDITELENFRKEQ
                     NKEAEKRKLDVKITPVVFIMKAVASALEAFPRFNSSISEDAQRLTLKKYINIGVAVDT
                     PNGLVVPVFKNVNKKGIIELSRELMEVSKKAREGKLTASDMQGGCFTISSLGGIGTTH
                     FTPIVNAPEVAILGVSKSEMQPIWNGKEFEPRLMLPLSLSFDHRVIDGADGARFLSYI
                     NSVLADIRRLVM"
     gene            5020..>6438
                     /gene="lpdA"
                     /locus_tag="PXA33_002085"
     CDS             5020..>6438
                     /gene="lpdA"
                     /locus_tag="PXA33_002085"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011272714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDL5121386.1"
                     /translation="MSKEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYSTLGGI
                     CLNVGCIPSKALLHVAKVIEEAKNAVHNGVTFGEPTIDLDQVRAGKEAVVSKLTGGLA
                     GMAKARKVTVVEGLAAFTGPNTLVARDRDGNPTTITFDNAIIAAGSRPIQLPFIPHED
                     PRVWDSTDALKLKEVPKKLLIMGGGIIGLEMGTVYNALGSEVEVVEMFDQVIPAADKD
                     VVAIYTKQIEKKFKLMLETKVTAVEAKDDGIYVSMEGKACNDTKRYDAVLVAIGRVPN
                     GKLIDAGKAGVEVDERGFIRVDKQMRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGMKHYFDPKVIPSIAYTEPEVAWVGKTEKECKQEGLNYEVAKFPWAASGRAIASEC
                     SEGMTKLIFDKDTHRVLGGAIVGSNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNAKAKK"
BASE COUNT         1958 a         1222 c         1501 g         1757 t
ORIGIN      
        1 aattttgtta tttatcacct caatcgttaa tttataagga agaatactat gtcagagaac
       61 ttaagaaatg acgtagatcc aatcgaaact caggattggt tagcatcact cgattcatta
      121 attcgtgaag aaggtttaga gcgcgctcaa tttatcattg aacaagttat tagccaagct
      181 cgtgcaggtg gcgtggcatt accaacaggc gtaacaacag attatgtcaa tactatccct
      241 gtttccgagc agccagctta tccgggtgat cataaaatcg aacgtcgtat tcgttctgcg
      301 gtacgttgga atgcgattgc ggcagtactt cgtagccaga agaaagatct tgacttaggt
      361 ggtcatattt caactttcca atctgcggca actatgtatg aagtgtgttt taaccacttt
      421 ttcaaagcac caaccgaaaa agatggtggt gatttagtgt tcttccaagg ccacgcagca
      481 ccgggtatgt atggtcgtgc gttcttagaa ggtcgtatta ccgcagaaca aatggataac
      541 ttccgtcaag aagcatttac agacggttta tcttcttacc cacaccctaa attaatgcct
      601 gaattttggc aattctctac cgtatctatg ggtttaggac cggtaaatgc tatttaccaa
      661 gctcgtttct taaaatattt agaaaaccgt ggtctaaaag atacttcggc tcaaaaagta
      721 tatgcattct taggcgatgg cgaaatggat gaaatcgaat ctaaaggtgc attatcattc
      781 gccggtcgtg aaaaattaaa taacttaatc ttcactatta gctgtaactt acaacgttta
      841 gacggtccgg ttaacggtaa cggcaaaatc gtgcaagaat tagaagcact ctttaccggt
      901 tgtggctggg aagtgattaa agtgttatgg ggtagtggtt gggataaact gcttgaaaaa
      961 gatacttcag gcaaacttaa acagttaatg atggaagtgc ttgatggtga ctatttaacc
     1021 ttcaaatcca aaaatggtgc ttacgttcgt gaacacttct tcggtcgtta ccctgaaact
     1081 gcagcattag ttgctgatat gacagacgat gaaatttggg cattacgccg tggtgcacac
     1141 gattctgaaa aactttatgc agcctatgca aaagcacaaa aatcggacaa accagttgta
     1201 attttagctc accaagtgaa aggctaccgt attcctgaag cggaaagtaa aaatactgct
     1261 caccaatcga aaaaaatgtc attagacagc ttaaaaggct tccgtgacta cttcgagcta
     1321 ccattaaccg atgagcaagt agaaagccta gagtatgtaa gattccctga aggttctgaa
     1381 gagtacaact acttacacgg acaacgtaaa gccttaaacg gttatgtgcc tgtacgtaaa
     1441 ccgaaattta cggttgattt caaagtgcct gaattaagcg aattccaagc attattggat
     1501 gagcaaccac gtggtatttc aaccacaatg gcgttctctc gtgtattgaa tactttattg
     1561 aaagataaaa atatcggtca gcaaatcgta cctattattg cggacgaagc ccgtactttc
     1621 ggtatggaag gtttattccg tcaaatcggt atttataacc cacatggtca aaactatgta
     1681 ccgtcagacc gtgatttagt agcttactac cgtgaagcaa aagatggcca agtgttacaa
     1741 gaaggtatca atgagttagg tgcaacagcc tcttgggtgg cagcagcgac ttcttattct
     1801 gttagcaact taccgatgat cccattcttc atctactact caatgtttgg tttccaacgt
     1861 gtaggcgata tgatgtggtt agccggtgac caattagctc gtggctttat gattggtggt
     1921 acttcaggtc gtacaacatt aaacggtgaa ggtttacaac acgaagatgg tcatagccat
     1981 attcaagcag gtgtaatccc gaactgcgtc acttatgacc ctgcatttgc ctttgaggtt
     2041 gcggttattg ttcaagacgg tattcgtcgt atgtatggtg aaaaccaaga agatatcttc
     2101 tactacatta ctaccttaaa tgaaattacc gaacagccgg caatgccggc aggtgcggaa
     2161 gaaggtatcc gtaaaggttt atataaattt gaaaccgttg agggtaaagg taaaggtcac
     2221 gtacaattat taagctcagg tgcaattatg cgtcacgtgc gtgcagctgc acaaattctc
     2281 gcgaatgact acggcattac agcagatgta ttctcagcac catcattcaa tgaattaggt
     2341 cgtgatggag cagatgtggc tcgttggaac ttattacacc caactgaaac accacgtgta
     2401 ccgtacgttg ctcaagtgtt acaagattta ccgactgttg cttcaaccga ctatatgaaa
     2461 atgtacgcag agcaaatccg tgcttatgta ccaagtaaac actatcacgt gttaggtaca
     2521 gacggtttcg gtcgttcaga cagccgtgca aacttacgtg aacacttcga agttgatgag
     2581 cgttatgtag tagttgcagc cctcgctcaa ttagcgaaag aaggtacagt tgaaaacaaa
     2641 gtggttgcgg aagctattac taaattcggc ttaaatgtag atcgtattaa cccattatat
     2701 gcgtaatttc taatactctc tcccttgatg ggagagagac agctaaaggt ttcgtttaga
     2761 aacttttagc agagagggta ttagttttat ttgcaaaaat gttgagaaaa tcaaccgctt
     2821 gcattccctt ctccctgatt tttcttctta caaagaaaaa tctgtccctc ccacaatggg
     2881 agagggggta actcaagaag gatttcaaaa tgtcaaaaca aattaatgtg ccggacatcg
     2941 gttcggatga agtcagtgtg actgaagtga tggtaaaagt tggtgatact atttcagtag
     3001 atcaatcaat tattaacgta gaaggcgata aagcctctat ggaagtgccg gctcctgaag
     3061 caggtgtggt aaaagaagtt ttagtgaaag tgggtgataa agtttcaacc ggttcaccaa
     3121 tgttaatttt agagtcagca gatgctgctc ctgctcaaga agcaccaaaa gcagaagcag
     3181 ctcctgctgc tccagtagca acaacttcag ccgttgttga agtaaatgta cctgatatcg
     3241 gtggcgatga agttaacgta accgaaatta tggttaaagt aggtgatagc gttgaagtgg
     3301 atcaatctat catcaacgtt gaaggtgata aagcctcaat ggaagtgcct gcaccggttg
     3361 ccggcgtagt aaaagaaatc ttaatcaatg tgggtgacaa agtttcaact ggtaaattga
     3421 ttatgaaatt tgaaacggct tccgcagcta cagcacaatc tacaccggtt gcggaagctg
     3481 ctccagctca agcttcggca tcagcgatta aagatgtaaa tgtaccggac atcggtggtg
     3541 atgaagtaaa cgtaactgaa attatggtta aagtgggtga ttctgtttca gaagaacaat
     3601 ctttaatcac ggttgaaggt gataaagctt caatggaagt tccggctcca tttgccggtg
     3661 tggtgaaaga aattttagtg aaatcaggtg ataaagtttc aaccggttca ttaattatga
     3721 aatttgaagt agcgggtgcg gcaccggctc cggcggctgc tcaagcggct tcagctcctg
     3781 ctcctgctcc acaagcagca gctccggcga aagctgacgc agctccggca ggtcaaaacc
     3841 aatcaggttt aagccaagaa caagtggtgg cttcagctgg ctatgcacac gcaacaccgg
     3901 taattcgccg tttagctcgt gagtttggtg taaacctaga caaagtaaaa ggtacaggtc
     3961 gtaaaggccg tatcgttaaa gaagatattc aagcatacgt taaaacggct gttcaagtat
     4021 ttgaaaaaca aggcggaaca gcagcggcag caacaggtgc ggcaaacggt gcaggcttag
     4081 gtttattacc gtggccgaaa gtagatttca gcaagtttgg tgaagttgaa gaagttgaat
     4141 taagccgtat caataagatt tccggtgcga acttacaccg taactgggta atgattcctc
     4201 acgttacaca tttcgatcgc acagacatta ccgagttaga aaacttccgt aaagagcaaa
     4261 ataaagaagc ggaaaaacgt aaattagacg tgaaaattac ccctgtggtc ttcattatga
     4321 aagcagtggc aagtgcatta gaagccttcc ctcgtttcaa cagctcaatt tctgaagatg
     4381 cacaacgttt aaccttgaaa aaatacatca atatcggggt tgcggttgat acgccaaacg
     4441 gtttagtggt gccggtgttc aaaaatgtga acaaaaaagg cattatcgaa ctttctcgcg
     4501 aattaatgga agtctcgaaa aaagcccgtg aaggtaaatt gacagcatct gatatgcaag
     4561 gtggctgttt cacaatttca agtttaggcg gtattggtac aacgcacttt acgccaatcg
     4621 tgaatgcacc ggaagtggct attttaggtg tgtctaaatc tgaaatgcaa ccgatttgga
     4681 atggcaaaga gtttgaacca cgcttaatgc ttccgctttc attatcattc gaccaccgtg
     4741 tgatcgatgg tgctgatggt gcgagattct taagctatat taatagcgtg ttggcagata
     4801 ttcgtcgttt agtgatgtaa ttcggtaaag ctcctcctaa aaaagtccct ctttagaaaa
     4861 gaggggtagg gggagatttg atagacgtaa tttacgaaga gaataaatta tttggtagaa
     4921 atttgtgttt gattgattgc tctctcactt cgctttatat tctgccaaat ctcccttacc
     4981 cctctttgct aaaggggggg aataaaaaga ggaaaaacta tgagcaaaga aattaaaacc
     5041 caagttgtcg tacttggtgc aggtccagca ggttactcag cggcattccg ttgtgccgac
     5101 ttaggcttag aaacagtaat tgttgagcgt tattcaacct taggtggtat ttgcttaaac
     5161 gtaggttgta tcccttctaa agcattatta cacgttgcaa aagtaattga agaagctaaa
     5221 aatgcagtac acaacggtgt gacctttggt gagccaacga ttgatttaga tcaggtacgt
     5281 gctggtaaag aagccgttgt ttcaaaatta accggtggct tagcaggaat ggctaaggct
     5341 cgtaaagtga cagtagtaga aggcttggct gcatttactg gtccgaatac gttagtcgct
     5401 cgtgatcgtg acggcaatcc tacaactatc acgttcgata atgcgattat cgcagcaggt
     5461 tctcgtccga ttcaactacc atttattcca cacgaagacc cacgagtttg ggattcaacc
     5521 gatgctctta aattaaaaga agtaccgaaa aaactcctta ttatgggtgg tggtattatc
     5581 ggtttagaaa tgggaacggt atataacgcg cttggttcag aagtggaagt ggttgaaatg
     5641 ttcgaccaag taattcctgc tgcagataaa gatgttgtag cgatttacac caaacaaatc
     5701 gagaaaaaat tcaagctaat gcttgaaacc aaagtaaccg cagtagaagc taaagatgac
     5761 ggtatctatg tatcaatgga aggtaaggct tgcaacgata ctaaacgtta tgatgcggta
     5821 ttagtcgcta tcggacgtgt gccaaacggt aaattaattg atgccggtaa agctggagtg
     5881 gaagtagatg agcgtggctt tattcgtgta gataaacaaa tgcgtaccaa tgtgccacac
     5941 atctttgcta ttggtgatat tgtcggccag ccgatgttag ctcacaaagg tgtacacgag
     6001 ggacacgtgg cagcagaagt gattgcagga atgaaacatt acttcgatcc gaaagtcatt
     6061 ccgtccatcg cttatactga accagaagtg gcttgggttg gtaaaactga gaaagagtgt
     6121 aaacaagaag gcttgaacta cgaagtggcg aaattccctt gggctgcttc aggtcgtgcg
     6181 attgcttctg aatgctcaga aggtatgacc aagttaattt ttgataaaga tactcaccgt
     6241 gtattaggtg gtgcgattgt tggtagcaat ggtggcgaat tattaggcga aatcggtctt
     6301 gcaatcgaaa tgggctgtga tgctgaagat atcgcattaa ccattcatgc tcacccaacg
     6361 ttacacgaat cggtaggatt agcggcagaa gtatttgaag gttcaattac agacttacca
     6421 aatgcgaaag ccaaaaag
//
LOCUS       DANLRK010000149         6142 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.149, whole genome shotgun sequence.
ACCESSION   DANLRK010000149 DANLRK010000000
VERSION     DANLRK010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6142
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.149"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            24..1940
                     /gene="iolC"
                     /locus_tag="PXA33_002086"
     CDS             24..1940
                     /gene="iolC"
                     /locus_tag="PXA33_002086"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HDL5121387.1"
                     /translation="MATVNHQDRPLDLICLGRVAVDFYGQQIGSRLEEMTTFSKYLGG
                     SSGNVAYGTAVQGLRSSMLARVGDEHMGRFLREELQSVGVDTSHLITDKERLTALVIL
                     GIKDKETFPLIFYRDNCADMAITKDDFSEAYIASSRCLAITGTHLSNPKTREAVLTAL
                     EYAGRNEVKRALDIDYRPVLWGLTSLGDGETRYIDSAEVTKSLQEVLHHFDLIVGTEE
                     EFHIAGGSTDTLTALKNVRKVSNAVLVCKRGALGCSVFEGELPNDLDGGLNVYGVRVE
                     VLNVLGAGDAFMSGLLRGYLNNEGWEQACHYANACGALVVSRHGCSPAMPTKRELDDY
                     LTRAESVPRPDLDERLNHLHRVTTRKKQWENLCIFAFDHRKQLVDMANKVGADLARIP
                     KLKQLLLKAMEQTATQSGLPQNQAGILADTTFGQDVLNEITGKDYWIARPIEEPSSRP
                     LELEYGDLGSQLASFPRDHVIKCLAFYHPNDESGLKARQDRKLKEVYQACCRTGHELL
                     LEIILPADMPQSEAFYNQAIAHFYQLGIKPEWWKLPDVSRAQWQAISATIEANDPDCR
                     GILILGLDAPLEVFKDTFEQAKGCEKVKGFAVGRTIFGEPSEKWLAGELDDNTLISAV
                     SEKYRTLIDLWQQR"
     gene            2229..3569
                     /locus_tag="PXA33_002087"
     CDS             2229..3569
                     /locus_tag="PXA33_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HDL5121388.1"
                     /translation="MSCNTSPKRFHASWESIKQHQVPEWYEDAKFGIFIHWGIYSVPA
                     FAPPTCQLGEIDIDEQWFCNNPYAEWYFNSINVKKGPTYEHHIKTYGESFAYNDFLPL
                     WKAEKWQPAEWAKLFAKAGAKYVVLTTKHHDGFCLYPSKYTDFNCMQAGPKRDLMGEL
                     TENVREQGLKMGAYYSGIIDWTYSSAPIFTESQNFSNACPTYEYADYAYKQVVELIDR
                     YKPDVLWNDIGWPKVGEHMLPHLFAHYYNSVQEGVVDDRWNKLWCDFTSKEYKHGVAS
                     RDKKWEMCRGMGLSFGYNQVEDESHLISVKDLISLLVETVADNGNLLLNIGPKADGTI
                     PQEQVERLLALGSWLEINGEGIYKSRCSQHQTEQIGDVTYRYTKVGADLYLYIDGLTE
                     AQISLPLSVYGELQALNTELKFTIENTAQGRVLHIQNYQPDWYVLGFKIADGEK"
     gene            3572..4912
                     /locus_tag="PXA33_002088"
     CDS             3572..4912
                     /locus_tag="PXA33_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL5121389.1"
                     /translation="MSVSQRFIDFMNAHVAPVARRMENQPHISAIRDGFIVVLPFLIV
                     GSFIMILLIPPFDENTQNAFGQAWWRFANWASPYGWNFFQMSFNAISLFTSASIAYNL
                     AKAYKREPLPAAFLSVMAFLLVAAPVKDGMMDIKFFGGIGLFSAIFIAIYSVEMTRLL
                     EFLKIKIRLPKEVPHAVAESLNIVIPILAILLTLYPFAIWVESATGRNIPQLIMDFMA
                     PLIAVSDSLGAICLFVIATHLLWFLGINGSLVLMQLWTPFLLQNMAANLAAFQAGEPL
                     PFIITNSFWDFYIVHGASGGVIALAFLLVRSKSAHLRSIGKIGLVPSFFSIGEPIVYG
                     VPMVVNPLFFIPLIFAPLANAVIAYLILDFNLIHRVYLMAPWTTPAPIGAYLVSAGDI
                     WAPILSIALIILDIVIYYPFFKMYEKVCVEKEKAQNLDEKAQQELLEKARMAAQ"
     gene            5050..5874
                     /locus_tag="PXA33_002089"
     CDS             5050..5874
                     /locus_tag="PXA33_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL5121390.1"
                     /translation="MNGPTQLAHFQEQIRKEYDGLSKRLKQVAQYVLDNGNSVVFDTV
                     STIAERADVPPSTLIRFANAFGFSGFNEIKQLYRQDMMEGTSRYTERVQLFRQIEPLD
                     EQNSELNHILDVFVQGNTQALQQLSTQVSEEQLAQTVDILHNAKRIFIVGLKRSFSIA
                     NYLNYALHHLDYDVFMIDGSGGMFDEQLSRIREGDAVIAVSFSPYANETLNVVTATAR
                     PGVKHIAITDSQLSPLLSFSDVSFIIKEAQVSGFRSQCATMTLAQTIAVSLALKKA"
     gene            complement(5918..>6142)
                     /locus_tag="PXA33_002090"
     CDS             complement(5918..>6142)
                     /locus_tag="PXA33_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIB"
                     /protein_id="HDL5121391.1"
                     /translation="ILEIINALGNKENIKQVEACLTRLRVVLHNNGLLNKSALKKLGA
                     VDVIKVGDTQQIIFGAKAGQYRDEINALIG"
BASE COUNT         1734 a         1312 c         1382 g         1714 t
ORIGIN      
        1 tttacttgtt gtaaggagcg gttatggcaa cagttaatca tcaagatcgt ccattggatc
       61 ttatctgttt agggcgtgtg gcggtggact tttatgggca acaaatcggt tcccgtttgg
      121 aagagatgac cacattttca aaatacctcg gcggctcgtc aggcaatgtg gcttacggta
      181 cggcggtgca aggtttacgt tcttcaatgc tggcaagggt cggtgacgaa catatggggc
      241 gttttctacg tgaagagcta caaagcgtag gcgtggacac cagccattta attaccgata
      301 aagaacgctt aactgcactg gttattctcg gcattaaaga taaagagact tttccgctga
      361 ttttctatcg tgataactgt gcggatatgg caatcaccaa agacgatttt agcgaagcct
      421 atattgcttc cagccgttgc ttagcaatta ccggcacgca cctgtcaaac cctaaaaccc
      481 gtgaagctgt gctgaccgca ctcgaatatg cgggtagaaa tgaggtaaaa cgagcgttag
      541 atattgatta tcgccctgtg ttatggggat taacctcact cggtgatggc gaaacccgtt
      601 atatagacag tgccgaagtc accaaatcgc tacaagaagt attgcaccat ttcgatctga
      661 ttgtcggcac agaggaagaa tttcatatcg caggcggttc gaccgatact ctcactgcac
      721 tcaaaaatgt gcgtaaagtc tctaatgccg tattagtgtg caaacgtggg gcattagggt
      781 gttcagtatt tgaaggcgag ctgccgaatg atttagacgg tggcttgaat gtgtacggcg
      841 tgcgtgtaga agtgctgaat gtgctgggtg caggcgatgc ttttatgtcg ggcttactgc
      901 gtggctactt aaataacgaa ggttgggaac aagcgtgcca ctatgccaat gcgtgcggag
      961 cgttagtggt atctcgtcac ggctgttcac cggcaatgcc gaccaaacgt gaacttgacg
     1021 attatttaac ccgtgcagag agtgtaccac gtccggattt agatgagcgt ttaaaccact
     1081 tacaccgtgt caccacccgc aaaaaacagt gggaaaatct ctgtattttt gcttttgatc
     1141 accgcaaaca gttggtggat atggcaaata aagtcggtgc agatcttgct cgcataccga
     1201 aactgaaaca gctgctatta aaagcgatgg aacaaaccgc aactcaatcc ggcttaccac
     1261 aaaatcaagc agggatccta gccgatacca ctttcgggca agatgtactt aatgagatta
     1321 ccggcaaaga ttactggatt gcccgcccga ttgaagagcc gtcttcccgc ccgctggaat
     1381 tagagtatgg agatttaggc tctcagctag catcattccc acgcgatcac gtcatcaaat
     1441 gcttggcatt ttaccacccg aatgatgaaa gcggtttaaa agcccgccaa gatcgcaagt
     1501 taaaagaggt gtatcaagcc tgttgccgta ccggtcacga gctattgttg gaaattattc
     1561 tgcctgctga tatgccacag agtgaggctt tctataacca agcgatagca catttctacc
     1621 aacttggcat taagccggaa tggtggaaat tgccggatgt ttctcgcgca caatggcaag
     1681 cgatttccgc caccattgag gcaaacgatc cggactgtcg tggtatttta attttaggtt
     1741 tagatgcccc gttagaggtg tttaaagaca cctttgaaca ggcaaaaggc tgtgagaaag
     1801 tgaaaggctt tgcagtcggc cgcaccattt tcggtgaacc gtccgaaaaa tggcttgccg
     1861 gtgaattgga cgacaatacc ttgatttctg cggtatcgga gaaatatcgc actttaatcg
     1921 atctctggca acaacgttaa tcatattgct ttaagtgaaa accccttagc ttatgatatt
     1981 aagcaagggg ttttgttttg caaaaaatcc ggctaaacag accgcttgct agccgtaaaa
     2041 atgcccgaat tttcctaaaa aagcgaatat cgggcattcc tcaatctgct tatcacaaaa
     2101 taagtgtgat gaagttcaca tttcatacat aaaccccccc ctttttatcg aaacaaattg
     2161 ttttattttt atcaaaatgg aaaatgtatt tcagtttagt tctatttaga ttcaatagga
     2221 gtctgtgtat gagttgcaac acttcaccaa aacgtttcca cgccagttgg gagtcgatca
     2281 aacagcacca agtaccagag tggtatgaag atgctaagtt tgggattttt attcactggg
     2341 ggatttactc cgtacccgcc tttgccccac cgacctgtca gttaggtgaa attgatattg
     2401 atgaacagtg gttctgtaat aacccttatg cagaatggta ttttaattcc atcaatgtca
     2461 aaaaaggccc gacctacgaa catcatatca aaacttatgg cgaatccttt gcctataacg
     2521 acttcctgcc actatggaaa gcggaaaaat ggcaaccagc agaatgggcg aaactgtttg
     2581 ccaaagctgg cgccaaatat gtggtattaa ccaccaaaca tcacgatggt ttctgtctct
     2641 atccaagtaa atataccgat ttcaactgta tgcaagccgg accgaagcgg gatttaatgg
     2701 gcgagctgac tgaaaatgtg cgggagcaag gcttaaaaat gggcgcttac tactcgggca
     2761 ttatcgattg gacttattcc tctgccccga ttttcaccga aagtcaaaac ttctcaaacg
     2821 cttgcccgac ttacgaatac gctgactacg cctataaaca agtggtagag ctgatcgacc
     2881 gctataaacc agatgtattg tggaatgata ttggctggcc aaaagtgggc gagcatatgt
     2941 taccacacct atttgctcat tattacaaca gcgttcaaga gggcgtagtg gacgaccgct
     3001 ggaacaaact atggtgcgat ttcacctcca aagaatacaa acacggcgtg gcttcccgtg
     3061 ataagaaatg ggaaatgtgc cgtggtatgg gcttatcgtt cggctataac caggtagaag
     3121 acgaaagcca tttgatttcg gtgaaagact tgatttccct attggtagaa accgtagccg
     3181 acaacggcaa tttattgctg aatatcggcc cgaaagcaga tggcactatt ccgcaagagc
     3241 aagttgaacg cttattagca ctggggagct ggctggaaat aaatggagag ggaatctata
     3301 aatcccgttg tagccaacac caaaccgagc aaatcggtga tgtaacctat cgttatacca
     3361 aagttggggc ggatctctat ctttacattg atggcttaac cgaagcccaa atcagcttgc
     3421 cactgtcagt ttatggtgag ctacaagcac tcaataccga gctgaaattt accattgaaa
     3481 acaccgcaca aggcagagtg ttacatattc aaaactacca gccggattgg tatgtattag
     3541 gctttaaaat tgcagatggg gagaaatagg tatgtccgtt tcacaacgtt ttattgactt
     3601 tatgaatgcc cacgttgctc cagtagcacg tcgaatggaa aatcaaccgc atatttccgc
     3661 tattcgagac ggttttattg tagtgttacc attcttaatt gtcggcagct ttattatgat
     3721 tttgctaatt ccgccatttg atgaaaatac ccaaaatgct ttcgggcaag cttggtggcg
     3781 ttttgcgaat tgggcaagcc cttacggttg gaatttcttc caaatgtcgt ttaatgcgat
     3841 ttcattattt acctctgcca gtatcgccta caaccttgcc aaagcctata aaagagagcc
     3901 cctgccagcg gcatttctct cggtaatggc atttttgtta gtggctgcac cggtcaaaga
     3961 cggaatgatg gatattaaat tctttggcgg tattgggcta ttctctgcga tttttattgc
     4021 catttattcg gtagaaatga cccgcttgtt ggagttctta aaaattaaaa tccgcttacc
     4081 gaaagaagtg ccgcacgctg tagcagaatc tctgaatatc gtgattccaa ttttagcgat
     4141 tttactgact ctctatccgt ttgccatttg ggtggaaagt gccacaggtc gtaatattcc
     4201 acaactaatt atggacttta tggcgccatt gattgctgtg agtgactcgc taggggcaat
     4261 ttgcttgttc gttattgcta cccatttgct ctggttctta ggcattaacg gctcattggt
     4321 actaatgcaa ctttggacac cattcctact gcaaaatatg gcggcaaact tagcagcatt
     4381 ccaagctggt gaaccgctcc cctttatcat caccaattcc ttctgggatt tctatatcgt
     4441 acacggggca agtggtggtg taatagcctt ggcattcctg ctggtacgca gtaaatcggc
     4501 acacttacgc tctatcggta aaatcggctt ggttccgtcc ttcttctcta tcggtgaacc
     4561 aattgtgtat ggtgtgccaa tggtggttaa tccgctgttc tttatcccgc tgattttcgc
     4621 accactagcg aatgcggtta ttgcctacct gattttagat tttaacctta ttcaccgtgt
     4681 ttacttgatg gcaccgtgga caacaccggc accgattggg gcatatctgg tttctgccgg
     4741 tgatatttgg gcaccaattt taagtattgc actgattatt ttggatattg tgatctacta
     4801 tccattcttc aaaatgtatg aaaaagtctg cgtcgagaaa gaaaaagcac aaaatctaga
     4861 tgaaaaagca caacaggagt tgctagaaaa agcacgaatg gcagcacaat aacacttatt
     4921 tctagcgtaa ttgagagggg ctaatcgaaa gattagccct tatttttgca aaaaattgac
     4981 aaaatttatc cgcttgtaaa caagaaaagc gtataatgaa ataaaaaatt caagcagaga
     5041 taatttgaaa tgaatggacc tactcaatta gcacattttc aagaacaaat ccgaaaagag
     5101 tatgacggac taagtaaacg tttaaaacaa gtggcacagt atgtgctgga taatggcaac
     5161 agcgtggtgt ttgatacggt ttctaccatt gctgaacgag ctgatgttcc cccttcaacc
     5221 ttaatccgtt ttgcgaatgc gtttggattt agcggtttta atgaaattaa acagctttat
     5281 cgccaagata tgatggaagg cacatcacgt tacaccgaac gtgtgcaact ttttcgtcaa
     5341 attgaaccgc ttgatgagca aaacagtgag ctcaatcata ttttagatgt gttcgtacag
     5401 ggtaatacgc aggcactgca acagttatcg acacaagtca gcgaagagca gttagcacaa
     5461 acggtagata ttttacacaa tgccaaacgc atttttattg tgggtttaaa acgctcattc
     5521 agcattgcga actacctaaa ctatgcgttg caccatttgg actatgatgt ctttatgatc
     5581 gatggttcgg gcggtatgtt tgacgagcaa ttaagccgaa tccgtgaagg tgatgcggtg
     5641 attgccgtga gcttttcccc gtatgcgaat gaaacactca acgtggtgac agcaactgcc
     5701 agaccggggg taaaacatat tgcgatcact gatagccaac ttagcccact cctttcattt
     5761 agcgatgtgt cgtttattat taaagaagca caggtgagtg gttttcgatc ccagtgtgcc
     5821 acaatgacgc tcgcacaaac gattgccgtt tcactggcgt tgaaaaaagc ctagattaag
     5881 aaagtgaaag acctgcgtaa atacaggtct ttgtctatta accgattaaa gcgttaattt
     5941 catcacgata ctgcccggct tttgcaccaa aaataatttg ttgcgtatca ccgactttaa
     6001 tgacatcaac cgccccaagc tttttcagtg ccgacttatt taataaaccg ttgttatgta
     6061 acaccacacg taaacgggtt aaacaagctt caacttgctt gatattctct ttgtttccta
     6121 aagcattaat aatttctaga at
//
LOCUS       DANLRK010000150         6116 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.150, whole genome shotgun sequence.
ACCESSION   DANLRK010000150 DANLRK010000000
VERSION     DANLRK010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6116
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.150"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            18..1031
                     /gene="cas1c"
                     /locus_tag="PXA33_002091"
     CDS             18..1031
                     /gene="cas1c"
                     /locus_tag="PXA33_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003762781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated endonuclease Cas1"
                     /protein_id="HDL5121392.1"
                     /translation="MRKLQNTLYITTQGSYLHKERETLVVEQDRKKVAQLPIHSIGHI
                     FCFGNVLVSPFLMGFCGENNVNLAFFTENGRYLGRLQGRQSGNVLLRRAQYKKSETNP
                     VPVARNIIAAKIQASKRVLQRQIRNHGENADLQSTITALNYSLQQLKTADNLDLIRGI
                     EGDAAARYFGVFSHLIKENSGFHFDGRNRRPPRDGVNALLSFLYSIVGKDISGALQGV
                     GLDPQIGFLHADRSGRDSLAQDILEEFRAWWVDRMVLSLINRGQVKPDDFVTEASGAV
                     TLKPEARKLLFQTLQAKKQEKIIHPFLEEEVEIGLLPYIQAMLLARHLRGDLAEYPPF
                     LMR"
     gene            1098..1391
                     /gene="cas2"
                     /locus_tag="PXA33_002092"
     CDS             1098..1391
                     /gene="cas2"
                     /locus_tag="PXA33_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005540845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HDL5121393.1"
                     /translation="MLMLITYDISFDDPEGQARLRRIAKHCLDYGVRAQYSVFECDVT
                     PAQWVTLKNKLLATYNPDCDSLRFYHLGSKWRNKVEHHGAKPSVDIFKDTLIL"
     repeat_region   1583..2685
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=1583..1602
                     /rpt_unit_seq="cagccacctacgcgtggctg"
     gene            2935..4410
                     /gene="rng"
                     /locus_tag="PXA33_002093"
     CDS             2935..4410
                     /gene="rng"
                     /locus_tag="PXA33_002093"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005610087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HDL5121394.1"
                     /translation="MSSTELLVNVTPSETRVALVENGILKELHIEREAKRGIVGNIYK
                     GRVTRVLPGMQSAFVDIGLEKAAFLHASDIVSHTECVDENEKKQFLVKDISELVREGQ
                     DIVVQVVKDPIGTKGARLTTDITLPSRYLVFMPENSHVGVSQRIESEEERARLKSLVE
                     PYCDELGGFIVRTAAEEATEESLKQDVEFLKRLWRKVLERKGKYSAKSMLYGELALAQ
                     RVLRDFIGTHINSVRIDSKLTYEQVREFLAEFMPELTDCVTLYTGQQTLFDAYGVEKA
                     IQKALDKRVDLKSGGYLIIEQTEAMTTIDINTGAFVGHRNLAHTIFNTNIEAIQAIAH
                     QLQLRNLGGIIIIDFIDMQEEEHRERVLQSLQEALKGDRVKTNVNGFTQLGLVEMTRK
                     RTRESLERILCCDCQACQGRGTVKTVETVCYEIMREIVRVHHLFKTENIHVYAAKDVA
                     EYLINEESHALLAELQAFIGKKIEIKLEPYYHQEQFDVVVM"
     gene            4564..6066
                     /gene="lysS"
                     /locus_tag="PXA33_002094"
     CDS             4564..6066
                     /gene="lysS"
                     /locus_tag="PXA33_002094"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HDL5121395.1"
                     /translation="MSEVENQELDLNGEMLARREKLNKIREQGNAFPNTFRRDALAKD
                     LHAQYDAVEGETLKEQNIQVKVAGRIMLKRVMGKASFFTIQDVSGAIQLYVARDNLSD
                     SVYEEKVSLWDLGDIVGVEGALFKTKTGELTVRCSEVQLLTKSLRPLPDKHHGLTDLE
                     TRYRQRYLDLISNEESRRTFMIRSQVVSGIRKFFLERDFIEVETPMLQVIPGGAAAKP
                     FITHHNALDVDMYLRIAPELYLKRLVVGGFERVFELNRNFRNEGVSVRHNPEFTMIEY
                     YQAYADYHDLMDNTEELLRQLALDILGTTDVPYGDYVFDFGKPFERITMHDAIVKYGN
                     GIQREDLDSFEKSVEIAKGLGIEIQKSWGLGSVVNAIFEEVAEHQLIQPTFLMAHPAE
                     ISPLARRNDENPEVTDRFELFIGGREIGNGFSELNDAEDQAERFDAQVAAKDAGDDEA
                     MFKDEDFIVALEHGLPPTAGEGLGIDRLVMIFANAPSIRDVILFPAMRQK"
BASE COUNT         1796 a         1130 c         1427 g         1763 t
ORIGIN      
        1 taaagggatt ttcaaccatg cgcaaactac aaaacaccct ctacattacc acccaaggta
       61 gctatttgca caaagagcga gaaaccttgg tggtggagca agatcggaag aaagtggcac
      121 agttgccgat tcattccatt gggcatattt tttgttttgg caatgtgtta gtgtcgccgt
      181 ttttaatggg attttgtggc gaaaataacg taaatttagc gttttttacc gaaaatgggc
      241 gttatcttgg gcggttgcag gggcggcaaa gtggtaatgt attgttacgc cgagcccagt
      301 ataaaaagtc ggagaccaac cctgttcctg tggctcgcaa catcattgca gccaaaattc
      361 aagcatctaa gcgggtgtta caacggcaaa ttcgtaatca cggcgaaaat gctgaccttc
      421 aatcgacaat taccgcatta aattacagcc tgcaacagtt gaaaacagca gacaatttgg
      481 atttgattcg tggcattgag ggcgatgctg cggctcgtta ttttggggtg ttttctcatc
      541 ttatcaaaga aaatagcggt ttccactttg acggacgtaa tcgccgccca cctcgtgacg
      601 gtgtgaatgc gttgctttca tttctttata gcattgtagg gaaagatatt agcggggcat
      661 tgcaaggcgt ggggcttgat ccccagattg gctttttgca tgcagatcgt tcggggcgag
      721 atagcttagc tcaagatatt ttggaggaat ttcgagcgtg gtgggtggac agaatggtgc
      781 tgtcgctgat caatcgaggg caggttaagc ctgatgattt tgtgacggaa gccagtggtg
      841 cggtaacgct caagcctgaa gccagaaaat tactatttca aaccttgcaa gcgaaaaaac
      901 aagaaaaaat catccaccca tttttagagg aagaagtgga aatcggcttg ttaccttata
      961 tccaagcgat gttattggct cgtcatttgc gtggcgattt ggcagaatat ccgccgtttt
     1021 taatgcgtta aaatctgcaa aattttgttg aaatttgacc gcttgtttat gttttattca
     1081 ttgaggaatt agcaactatg ctgatgttga ttacctacga catttcattt gatgaccccg
     1141 aagggcaggc tcgcctacgc cgtattgcta agcattgttt ggattatggt gtgcgggcgc
     1201 aatattctgt gtttgagtgt gatgttactc ctgctcagtg ggtaacattg aaaaataaat
     1261 tattggcaac ttacaaccca gactgtgata gtttgcgctt ttaccattta ggcagtaaat
     1321 ggaggaataa agttgagcac catggcgcaa aaccttcagt ggatattttt aaagatacgc
     1381 tgattttata aatcgctagc ctgtagttct cataaaaatg tagcaaggtt agcgaatcta
     1441 tattgttctt taataatcaa ggaattagaa aaaataaggc acaatatatt ggcgatatgc
     1501 tatactttat ccactctcta caaaacgtta gcgaataaag ggctaaaaag cctgatattt
     1561 caatacgtta tcttataggg accagccacc tacgcgtggc tgcgaattga aaccctataa
     1621 cgagcaatgg cattttgatc tacggtaaac cagccaccta cgcgtggctg cgaattgaaa
     1681 cttcttgaat acctccggag tcagttaaga taaggccagc cacctacgcg tggctgcgaa
     1741 ttgaaacctt tttcaagaat caagattaag agatccaaac aaccagccac ctacgcgtgg
     1801 ctgcgaattg aaaccaccgc tgctacttcg gttatcggag tagtttgcac ccagccacct
     1861 acgcgtggct gcgaattgaa acgtggcaca gtaacacgag cagatgctga taaacgccca
     1921 gccacctacg cgtggctgcg aattgaaacc agcagccgtt gttaccgtgg tgcaatgctt
     1981 gcgccagcca cctacgcgtg gctgcgaatt gaaactacac ttattgccat agggtagtag
     2041 taatctttta cccagccacc tacgcgtggc tgcgaattga aactgcgatt tttggatttt
     2101 tcgggcgata aatgatacca gccacctacg cgtggctgtg aattgaaact cgtggctggt
     2161 tatacaaaga aaagcattgt tgggcagcca cctacgcgtg gctgtgaatt gaaacttaaa
     2221 tctaaagcta aagctatttt attaattaaa gcagccacct acgcgtggct gtgaattgaa
     2281 actttaccgt ttaccgattg gattttaatg ctagaggcag ccacctacgc gtggctgtga
     2341 attgaaaccc taaattgccg taaaaatctg ccattagcta cgcgcagcca cctacgcgtg
     2401 gctgtgaatt gaaacctcat aacgaatcca atcctgttcg gttaatcccc gcagccacct
     2461 acgcgtggct gtgaattgaa acaggggcgt gtaccgcaat caattttaat tataaccaag
     2521 cagccaccta cgcgtggctg tgaattgaaa ctttatctgt tgcggtcaat tgtatatctt
     2581 gaatttgcag ccacctacgc gtggctgtga attgaaacct cgtcttgaat tggaaaatca
     2641 agggcgacaa taggcagcca ccttcgcgtg gctgtgtatt gaaacggtgt acccacaaat
     2701 cctatgtttt cgccattggc tgctaccttc gcatcaatat atattgaaat atcaatggca
     2761 aatttgtatg ttgttaatta gactgattgc atctgctatg tatcatagta atgacgaaaa
     2821 tattctattt tgcaaaaaat aatcagaaat tacccgcttg ttcgaactga gtttttgttt
     2881 tctaagatgt gaaatgatac actttagata gttttaaaat atagagacag taagatgagt
     2941 agtacggaat tattagtgaa tgttacccca agcgaaacaa gggtagcgtt ggtagaaaac
     3001 ggaatattga aagaattaca tattgagcgg gaagctaaac gcgggattgt tggtaatatt
     3061 tataagggaa gggtgacaag ggtattacca ggtatgcaat ctgcttttgt ggatatcggc
     3121 ttggagaagg ctgctttttt gcacgcttct gacatcgttt cgcatacgga atgtgttgat
     3181 gaaaatgaaa agaagcaatt tctggttaaa gatatttctg aattggtgcg tgaagggcaa
     3241 gatatagttg tacaagtagt gaaagatcca attggtacta aaggagctag gctcactact
     3301 gatattacgt taccatctcg ttatttagtg tttatgccag aaaatagcca tgtgggagta
     3361 tctcagcgga ttgaaagtga agaagagcgt gctcgtttaa aaagcttggt tgagccttat
     3421 tgtgatgaac ttgggggctt tattgtacgt accgctgcag aagaggcaac tgaagaaagt
     3481 ttaaagcaag atgttgaatt tttaaaacgg ttgtggcgta aagtattaga acgaaaaggg
     3541 aaatattccg caaaatcaat gctttatgga gagcttgcgc ttgctcaaag ggtactgcgt
     3601 gattttattg gtacacatat caattcggtt agaatcgatt caaagctaac ttatgagcaa
     3661 gtgcgagaat ttttagctga gtttatgcca gaattaacgg attgtgtcac cctctatacc
     3721 ggtcagcaaa ctttatttga tgcctatggt gtagaaaagg caatccaaaa ggcattggat
     3781 aaacgggttg atttaaaatc ggggggctat ttaattattg agcaaactga ggcgatgacg
     3841 actattgata ttaatactgg tgcttttgtc ggacatcgta accttgcaca tacgattttc
     3901 aacacaaata ttgaagcaat acaggcaatt gcacaccagc tacagttgcg taatttaggt
     3961 ggtattatca ttattgattt tattgatatg caagaggaag aacatcgtga gcgcgtttta
     4021 caatcgttac aagaggcatt aaaaggcgat cgtgttaaaa ccaatgtgaa tggctttact
     4081 cagcttggct tggtggagat gaccagaaaa cgtacaaggg agagtctaga gcgaattctt
     4141 tgttgcgatt gccaagcttg ccaagggcgt ggcacagtga aaactgtaga gacggtttgc
     4201 tatgaaatta tgcgtgaaat tgtgcgtgta catcatttgt ttaagacaga aaatattcac
     4261 gtttatgcag ctaaagatgt tgctgaatat ttaattaacg aggaaagcca cgcattattg
     4321 gctgagcttc aggcgtttat cggtaaaaaa attgagatta aacttgagcc atattatcat
     4381 caagagcagt ttgatgtggt cgtgatgtaa tctattactt ggttgaggct aaatagttag
     4441 cctcaatatc gttagttaca aaaaatttag aaaatctgac cgcttgtttc ctatccaaat
     4501 tcctcaaaat aacgtataat cgtccagttt tatttatcct tttttgaaat ctaaggtaat
     4561 tcaatgtctg aagtagaaaa tcaagaatta gatctaaacg gtgaaatgtt agcccgccgt
     4621 gaaaaattaa acaaaatccg tgagcagggt aacgcatttc caaatacatt ccgtcgtgat
     4681 gcgttagcca aagatctgca cgctcaatat gatgcagtgg aaggtgaaac cttaaaagaa
     4741 cagaatattc aagtgaaagt tgccggtcgt attatgttaa aacgagtaat gggtaaggca
     4801 tctttcttta ccattcaaga tgtgagcggt gcgattcagc tttatgtagc tcgtgataac
     4861 ttaagtgaca gcgtttacga agaaaaagtc agcctttggg atttaggcga cattgtgggc
     4921 gtcgaaggtg cgttatttaa aaccaaaacc ggtgaattaa cggttcgttg ctctgaagtt
     4981 caacttttaa ccaagtcttt acgcccgttg ccggataaac atcacggttt aacggattta
     5041 gaaactcgtt atcgtcagcg ttatttagac ttgatttcta atgaagaatc tcgccgtact
     5101 tttatgatcc gctcgcaagt agtttcaggc attcgcaaat tcttcttaga aagagacttt
     5161 attgaagttg aaacgccaat gttgcaagtg atcccgggcg gtgcggcagc gaaaccgttt
     5221 atcactcatc ataatgcgtt agatgtggat atgtatctgc gtattgctcc agagctttac
     5281 ttaaaacgtt tagttgtggg tggttttgag cgagtatttg agcttaaccg taacttccgt
     5341 aacgaagggg tttcagtacg ccacaatcct gaatttacaa tgattgagta ctaccaagcc
     5401 tatgcggatt atcacgattt aatggataat accgaagaac tgttacgcca attagcctta
     5461 gatattcttg gcacaactga cgtaccttat ggtgattatg tattcgattt cggtaaaccg
     5521 tttgagcgta ttacgatgca cgatgcgatt gtgaaatatg gtaacggcat tcaacgtgaa
     5581 gatttagaca gcttcgagaa aagtgtggaa attgcgaaag gcttaggcat tgaaattcaa
     5641 aaatcttggg gtttaggctc ggtagtcaat gcgatttttg aagaagtggc agaacatcaa
     5701 ttaattcagc caactttctt gatggctcac ccggcagaaa tttcaccatt agctcgccgt
     5761 aatgatgaaa acccagaggt aactgatcgt tttgagttat ttatcggtgg tcgtgaaatt
     5821 ggtaatggtt tctcagaatt aaacgatgca gaagaccaag cagagcgttt tgatgctcaa
     5881 gttgcggcga aagatgccgg tgatgatgaa gcaatgttta aagatgaaga ctttatcgtg
     5941 gcattggaac acggtctgcc gccaacagcg ggtgaaggtt taggaattga ccgcttagta
     6001 atgattttcg ctaacgcccc atcaatccgt gatgtaattt tattcccagc gatgcgtcag
     6061 aagtaatcag aaatagaaca aaagcacact tcggtgtgct tttttgtatt acaagc
//
LOCUS       DANLRK010000151         6110 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.151, whole genome shotgun sequence.
ACCESSION   DANLRK010000151 DANLRK010000000
VERSION     DANLRK010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6110
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.151"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            149..361
                     /locus_tag="PXA33_002095"
     CDS             149..361
                     /locus_tag="PXA33_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL5121396.1"
                     /translation="MSAITGTVKWFNATKGFGFITPDQGGKDVFVHFSAIEANGGYRT
                     LEDGAKVQFEIQDSERGASAVNVKTI"
     gene            complement(421..1575)
                     /locus_tag="PXA33_002096"
     CDS             complement(421..1575)
                     /locus_tag="PXA33_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HDL5121397.1"
                     /translation="MMKVSPFQWAAFNYFGFFCAYGVLLPFLPVWLKHHGYSTEMIGL
                     FAAVGYLFRFAGSMLASQRVKTVSQLIPTARSLTWLNIAAAAALLFSGDFIWLLFPVL
                     MLFQMFNSGAMPIADSIASIWQHQIGLDYGRARLFGSMAFVVGSLSAGYLIGLLGENS
                     VVWIIIGFLVFLGIGQLATPKVGFEEQGNKANTSSVTYRQLLKEPETLKMLIAVSLIS
                     ASHAAYYTYSTIHWSNASISTAMTSLFWGLAVVAEILFFLVSKRLFKSWKISHLMILA
                     AVFAVIRWAIMGSTTEIVLIIVMQMLHSITFGAAHIAMIRYISMQSAERVTKLQGLYF
                     GLASCAFMAVFTFIAGMVYQHSPSLTFWLMAIFTLPAIFIVPKRFEVQIN"
     gene            complement(1572..2390)
                     /locus_tag="PXA33_002097"
     CDS             complement(1572..2390)
                     /locus_tag="PXA33_002097"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HDL5121398.1"
                     /translation="MNEKKLAFIGTGNMAYAIIQGLLKSGYSAEQIIACNKSNLARRE
                     ELQAVGIATNFANREAVEQADVIVLAVKPQMMAQVCAEFADVDFSNKWVISVAAGISV
                     QRLETLLPSAKNIIRTMPNTPSLISEGMTGLFAKKSVNPTACQFAEQLMSAVGKCYWV
                     ETEEQINHIIAITGSSPAYFFRFMEAMQQSAVQMGFSEQDARTLVQSVALGAAKLVEA
                     NPTTPLSTLRENVTSKGGTTAKALEVFEQHDLAKTVDDAMWAAIHRAREMEQSL"
     gene            2467..3375
                     /gene="rdgC"
                     /locus_tag="PXA33_002098"
     CDS             2467..3375
                     /gene="rdgC"
                     /locus_tag="PXA33_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HDL5121399.1"
                     /translation="MVWFKNVMIYKLTGELALESNSLNELQQETRFTPCSQHDMSKFG
                     WATPLTGSDLLHFSQANQFLLISHKEDKLLPANVVKAETEQRIAELEEKEARKLKKTE
                     KQAVKDQVVSALLPRAFSKHQFTALWLDLDNQLVYVDAASSKRAEDTLALLRKTLGSL
                     PVVPISFTLPPAEVMTNWIAKGHTPSWLNLLEEAELKSFDTDSIIRCKRQDLETEEIA
                     RHLQAGKFITKLAIEWEGHFNCVLNEDATLSRLKFADEVREKNDDILKEDVAQRFDAD
                     FLLMTEELKLFTEKMIAEFGGIKSRI"
     gene            3396..4001
                     /locus_tag="PXA33_002099"
     CDS             3396..4001
                     /locus_tag="PXA33_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase family hydrolase"
                     /protein_id="HDL5121400.1"
                     /translation="MLDQTIIDQYDALIFDMDGTLIDTMPSHAKAWEKVGEVLGYPIN
                     PKPMYELSGSTTFVIAREIMQRSNIPEHYFEQVVQLKREFGIEMVLANATLLPAFEII
                     KANVGKKPMAIGTGSHRAMVELLDQKFNLRQYVSVIVDSDDVSNHKPAPETFLKCAEK
                     LGIAPHRCLVFEDADLGVQAALNGNMNVFDVRTNTITHSTR"
     gene            4017..4808
                     /locus_tag="PXA33_002100"
     CDS             4017..4808
                     /locus_tag="PXA33_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HDL5121401.1"
                     /translation="MKTEWIKHLIQQLTTLSKAIENYFTSGSFGVKFYPFLMHPCFRK
                     SCAFLIFIIAMVSSFSGLFYLVAEHITILFSERSLTTYFHHSTLELMIPVGTMIDEKI
                     SELSPLSVVMRSMFVLQAVVFFFIYAIGITHIAHNKLRVIGLLLSLCFAIGCSLVAGI
                     QPSSRGEFGIYNLGASITFLIGNLTLIIAGFDLYHPSMQFFKRFSIGAGVIGAICILI
                     TIFLPTLFSPLIERAGIYTIMLWEILAGFAIIKRLLKLPSFRQQG"
     gene            4812..5954
                     /gene="argE"
                     /locus_tag="PXA33_002101"
     CDS             4812..5954
                     /gene="argE"
                     /locus_tag="PXA33_002101"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016533842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HDL5121402.1"
                     /translation="MKQIPFIQRYAQLIELPTISSLVAEEDLSNRRLIELLATWLADF
                     GFKTEILAVEGSRNKYNLLATYGEGEGGLLLAGHTDTVPFDEGKWRFNPFQLTEKAGK
                     LYGLGTADMKGFFAFVVEVVSQLDLTQIKKPIRILATADEETTMLGARTFAQHSHIRP
                     DCAIIGEPTSLKPIRAHKGHIGESVRITGRSGHSSDPAKGINAIELMHQSIGYLMKMR
                     DELREKYHNPLFQVTHPTMNFGNIHGGDAINRICACCELQFDMRPLPNMAVQDLYAMV
                     NEHLKPMLEQYGDLIEIRHLHDGIPGYECEHSAQIVQVVEKLLGEKCDAVNYCTEAPF
                     IQQLCPTLVLGPGSIEQAHQPDEFLEMKYIEPTKELLTKLIYHFCG"
BASE COUNT         1795 a         1401 c         1298 g         1616 t
ORIGIN      
        1 agtggtgtaa tggctcaagc tgttcgtgcg ttaattaaag ttgaatcttg ttagttattt
       61 tcctcaaggc aagtgattga tagatacgtc tgtaatatcc cgtttagcta tcttttatca
      121 ctttttgtat ttatataagg aaaattttat gtcagcaatt accggcacag taaaatggtt
      181 caacgccacc aaaggttttg gttttatcac ccctgatcaa ggtggcaaag atgtattcgt
      241 acacttctct gctatcgaag caaacggcgg ctaccgcaca ttagaagacg gtgctaaagt
      301 acaatttgaa atccaagatt cagagcgtgg agcttcagcc gtaaacgtga aaacaatcta
      361 atttcagcaa attcaatgaa tttcgataaa aatgccgaca acttgtcggc atttttgcta
      421 tcaattaatc tgtacttcaa accttttcgg cacaataaaa atggcaggta gggtaaaaat
      481 tgccatcaac caaaatgtca ggcttgggga atgttgatag accattccgg caataaaggt
      541 aaatactgcc ataaaagcac agcttgccaa gccaaaatag agaccttgta atttcgtcac
      601 acgctctgcc gattgcatcg aaatatagcg aatcatcgca atatgagccg ccccgaaggt
      661 aatagaatgt agcatttgca ttacaataat cagcacgatc tctgtagtac tacccattat
      721 tgcccaacga atcactgcaa aaacggcagc taaaatcatt aaatggctga ttttccacga
      781 tttaaacaag cgtttagaga ccaaaaagaa caaaatttcc gccaccaccg ccaagcccca
      841 aaacaggcta gtcattgcgg ttgaaatact ggcatttgac caatgaatgg tgctgtaagt
      901 gtaataagcg gcgtgcgagg ctgaaattaa cgacaccgcg atcaacattt ttaaggtttc
      961 cggctctttc aataattggc gataagttac gcttgaagta ttggctttat taccttgttc
     1021 ttcaaacccg actttgggcg tagcaagctg ccctattcct aaaaaaacca agaaaccgat
     1081 gataatccac accaccgaat tttcaccaag caagccgata agatagccgg cagaaagtga
     1141 gcctaccaca aacgccattg agccgaataa ccttgctctg ccgtaatcca gcccgatctg
     1201 atgttgccaa attgaggcaa tgctatcggc tatcggcatt gcccccgaat tgaacatttg
     1261 aaacaacatt aacaccggaa aaagtagcca aatgaaatcc cctgaaaaca gcaacgccgc
     1321 cgcagccgca atattcagcc aagttaaact gcgggcggtg ggaatgagtt ggctcacggt
     1381 ttttacccgt tgtgaggcaa gcatactgcc ggcaaagcgg aacaaatagc caacggcggc
     1441 aaataagccg atcatttcag tcgaatagcc gtgatgttta agccacactg gcaaaaacgg
     1501 caacaacacg ccataggcac aaaaaaagcc aaagtaatta aatgccgccc attggaacgg
     1561 ggaaactttc atcataacga ttgctccatt tctctggctc gatgaattgc cgcccacatt
     1621 gcatcatcaa cggttttagc aagatcgtgc tgctcaaaca cctcaagggc tttcgccgtt
     1681 gtgcctccct ttgaggtcac attttcccgc aaggtcgaaa gtggcgtagt cgggttcgct
     1741 tccaccaatt tcgccgcccc caatgcgaca gattgtacaa gcgttcttgc gtcctgctcg
     1801 gaaaagccca tttgcaccgc actttgctgc attgcctcca taaaacggaa gaaatacgcc
     1861 gggcttgagc cggttatcgc aataatgtgg ttgatttgct cctcggtttc cacccagtaa
     1921 catttaccaa cggctgacat caactgttcg gcaaattggc aagcggtcgg atttaccgat
     1981 ttttttgcaa ataatccggt cattccttcg ctgattaaag acggggtgtt tggcatcgtg
     2041 cgaatgatat ttttcgcact cggcaacagc gtttccaagc gttgcacaga aatacctgct
     2101 gctactgaaa tcacccattt gttggaaaaa tcaacatcgg caaactcggc acacacttgt
     2161 gccatcatct gtggttttac cgccagcacg atcacgtcag cctgctctac cgcctcacga
     2221 tttgcaaaat ttgtcgcaat tccgaccgct tgtaactcct ctcggcgagc caaattactt
     2281 ttattgcacg caatgatttg ttcggcagaa taaccgcttt ttaataagcc ttgaataatc
     2341 gcatacgcca tatttccggt gccgataaag gcaagttttt tctcgttcat tcgcaaattc
     2401 tctactgtgt tgggttgagg tctgttaaaa taacactatt ctacacccga taaaggatat
     2461 gcaaaaatgg tttggttcaa aaatgtgatg atctataaat taaccggcga gctggcgttg
     2521 gaaagcaata gccttaacga gttacagcaa gaaacccgct ttaccccttg cagccagcac
     2581 gatatgagca aattcggctg ggctacgcca cttaccggct cggatttact gcatttctca
     2641 caagcgaacc aatttttgct gatttcacac aaagaagata aattattacc tgccaatgtg
     2701 gtgaaagccg aaaccgagca acgcattgca gaactggaag aaaaagaggc tcgtaagctg
     2761 aaaaaaaccg aaaaacaagc cgtgaaagat caggtcgttt ctgcgttatt gcctcgagca
     2821 ttcagcaaac atcaattcac cgctctttgg ttggatttag ataatcagtt ggtttatgtg
     2881 gatgcagcct cgagcaaacg ggcggaagat acgctggcgt tactgcgaaa aacacttggc
     2941 tcgttgccgg ttgtgccgat ttcctttacc ctaccgccgg cagaggtaat gacgaattgg
     3001 atcgccaaag ggcatacgcc aagttggttg aatttgttgg aagaggcaga actgaaatct
     3061 ttcgataccg acagcattat tcgctgtaaa cgccaagatt tagagacgga agaaatcgcc
     3121 cggcacttgc aagcgggcaa atttatcaca aaacttgcga ttgaatggga agggcatttc
     3181 aattgcgtgt taaatgaaga cgccacgctt tcacgcctga agtttgccga tgaagtgcga
     3241 gagaaaaatg acgatattct caaagaagat gtggctcaac gctttgatgc cgatttctta
     3301 ttaatgaccg aagagctgaa acttttcacc gaaaaaatga ttgccgaatt tggcggaatt
     3361 aaatccagaa tttagaaaaa aatttaagga aagcaatgtt agatcaaacc attatcgacc
     3421 aatatgacgc cttgattttt gatatggacg gcacattaat tgacacgatg ccaagccacg
     3481 ccaaggcgtg ggaaaaggtg ggcgaagtgt taggctaccc aatcaatcca aagccaatgt
     3541 acgaactgag tggctcaacc acttttgtga ttgcccgaga aataatgcaa cgctccaata
     3601 tccccgagca ttattttgag caagtggtac aactaaaacg ggaatttggg attgaaatgg
     3661 tgttagccaa tgccactctg ttgcctgcct ttgagattat taaagctaac gttggcaaaa
     3721 aaccaatggc aatcggcaca ggctcacacc gtgcaatggt ggaattatta gaccaaaaat
     3781 ttaacttacg tcaatatgtt tcggttattg tagattctga tgatgtaagc aaccataagc
     3841 cagcccctga aaccttttta aaatgtgcgg aaaaacttgg cattgcccca caccgctgtt
     3901 tggtgtttga agatgccgat ttaggcgtgc aagccgccct caacggcaat atgaacgtgt
     3961 ttgatgtacg tacgaatacg ataactcact caacccgtta aaaatgatag gtttatatga
     4021 aaacagaatg gattaaacat ttaattcaac aattaaccac gctttccaaa gccatcgaaa
     4081 actactttac cagcggttca tttggggtga aattctaccc ttttctaatg cacccttgtt
     4141 ttcgtaaaag ctgtgccttt ttgattttta ttattgctat ggtttcaagt tttagcgggc
     4201 tattttattt ggtggcggaa cacatcacga ttttatttag cgaaagatcg ctcaccactt
     4261 acttccacca tagcaccctt gaattgatga ttccagtcgg cacaatgata gatgaaaaaa
     4321 tcagcgaatt atcaccactt tcggtggtaa tgcgttcaat gtttgtgcta caagcagtcg
     4381 tttttttctt tatttatgca atcggcatca cccacattgc tcacaacaaa ttgcgggtca
     4441 tcggcttatt gctttcgcta tgctttgcga ttggctgctc actagttgcc ggtattcagc
     4501 caagcagtcg tggcgaattc ggcatttaca atttaggggc aagcattacg tttttaatcg
     4561 gcaatttaac gctgatcatc gcaggcttcg atctctacca cccttcaatg caatttttta
     4621 aacgctttag cattggtgcg ggcgttatcg gggcaatctg tattttaatc acgattttcc
     4681 taccaaccct gtttagcccg ttaattgaac gagcaggtat ttataccatt atgctgtggg
     4741 aaattttagc cggttttgcc atcattaaac gcctgttgaa attgccctca ttcagacaac
     4801 aaggataacc aatgaaacag attcccttta ttcaacgcta tgctcaactg attgagctac
     4861 cgaccatttc cagccttgtt gccgaagaag atttatcgaa ccgccgtttg attgaattgc
     4921 tcgccacttg gctggcagat ttcggcttta aaaccgagat tttagcggta gaaggcagcc
     4981 gtaataaata caatttactt gcgacctatg gcgagggtga gggcggctta ttattggcag
     5041 ggcataccga caccgtgccg tttgatgaag gaaaatggcg cttcaatcca ttccaattaa
     5101 ccgaaaaagc aggcaaactc tacggcttag gcacggcaga tatgaaaggc tttttcgcct
     5161 ttgtggtgga ggttgtgagc caactcgacc taacccaaat caaaaagccg attcgcattc
     5221 ttgccaccgc tgatgaagaa accacaatgc tgggggcaag aacctttgct cagcacagcc
     5281 acattcgccc cgattgtgcg attattggcg agccgacctc gctcaaaccg attcgagccc
     5341 acaaagggca tattggcgaa tcggttcgca ttaccggcag aagcggacac tccagcgacc
     5401 ccgccaaagg cattaatgcg attgaattaa tgcaccaatc tatcggttat ttaatgaaaa
     5461 tgcgtgatga actgcgagag aaataccaca atccgctgtt ccaagtcacc cacccgacaa
     5521 tgaacttcgg caatattcac ggcggcgatg ccattaaccg catttgtgcc tgttgcgaat
     5581 tgcaatttga tatgcgacca ctaccgaata tggcggtgca agatttgtat gcaatggtaa
     5641 acgagcattt aaaaccgatg ctggaacaat atggcgattt aattgaaatc cgccatttac
     5701 acgatggaat tccgggctac gaatgcgaac actcggcaca aatcgtgcaa gtggttgaaa
     5761 agctactggg cgaaaaatgc gatgccgtca attactgcac cgaagcccca ttcattcaac
     5821 aactctgccc aacgctggta ctaggccccg gctccattga acaagcccac cagcccgatg
     5881 agtttttgga aatgaaatat atcgaaccga caaaagagtt attgactaaa ttgatttatc
     5941 atttttgtgg ataaatcaac agaaacaagc gctcattttc gccatacttt ttgcaaaaga
     6001 atcggcaaaa atgaccgctt gatatttagt attgagtgac ctataaactt ccatacttca
     6061 tcattgattt aaggagattc tgttagatag gtattaatct ctagctaggt
//
LOCUS       DANLRK010000152         6087 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.152, whole genome shotgun sequence.
ACCESSION   DANLRK010000152 DANLRK010000000
VERSION     DANLRK010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6087
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.152"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            <1..186
                     /gene="zapB"
                     /locus_tag="PXA33_002102"
     CDS             <1..186
                     /gene="zapB"
                     /locus_tag="PXA33_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011201404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapB"
                     /protein_id="HDL5121403.1"
                     /translation="KIKQAVETIQLLQLEVEELKGKNDAAKQENDTLRSEYEQLKVEQ
                     QNFQDRLRSLLGQIDNV"
     gene            242..823
                     /locus_tag="PXA33_002103"
     CDS             242..823
                     /locus_tag="PXA33_002103"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014064645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HDL5121404.1"
                     /translation="MAKLYFYYSSMNAGKSTTLLQSSYNYQERGMNTLVYTAAIDDRF
                     GVGKVNSRIGISQEANLFTADTNLFAEIKALHSNKALHCILIDEAQFLTKTQVYQLSD
                     VVDKLKIPVLCYGLRTDFQAELFEGSKYLLAWADELEELKTICDCGKKAHFVVRLNEK
                     GEAIKDGEQIQIGGNDRYLSVCRYHYKQKLGKL"
     gene            complement(914..2032)
                     /locus_tag="PXA33_002104"
     CDS             complement(914..2032)
                     /locus_tag="PXA33_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HDL5121405.1"
                     /translation="MTTTYFHLMAKPSSFHCNIQCEYCFYLSKEQTIPPEKSQFMNDE
                     TLQNYIRHYIEANQSQRVDFVWQGGEPTLLGLDFYKQAVKYQQKFANGKQITNAIQTN
                     GIALNRQWASFFKENHFLVGLSIDGLSDIHDRYRISTNGKPTFERVKSAVDLLKEVGV
                     EFNTLTVVNDRNWQRGRETYQALKALGSRYMQFIPVVESHKGELQPFSVPPQGYGQFL
                     VDVFHEWLPDRGQIFVIEFDNLLGQWLGYPSNNCVFRPTCGSSLVVEACGDVYSCDHF
                     VYPDYHIGNLNQQQLAEIVSSAAQRQFGQNKQAKLSQACLNCKFRILCHGGCPKHRIV
                     PQANSENHNYLCPSYQQFFAETSSVMRQMSEQIRFAGK"
     gene            complement(2122..3570)
                     /locus_tag="PXA33_002105"
     CDS             complement(2122..3570)
                     /locus_tag="PXA33_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005822114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HDL5121406.1"
                     /translation="MNNIIYILLDQVRKDMLGTYGHQVVKTPNLDRLAKDGIRFNNAF
                     TPASVCGPARTSLFTGLMPSSHGIIKNGEKGGTGEIPQQNPNIAGVEGYNSFVIGKWH
                     VGTKSVPEDYGIAGHNFDGYGYPGSQVYKNLVFNQPPTHSNRYREWLAEKGYDAPEVS
                     RAYFGDNPHLRVQELCGLLSGSREQTIEWFIIDEAKKYIQASLDEKKPFFAWINFWGP
                     HTPCVVPEPYYSMYDPSQVVLDESFFKPLEGKPNHFRTISQMWGMWEASEDRWKEVIS
                     KFWGYITLIDDAIGELFRFLEENNLYENTFLVATADHGDAMGAHKMIEKGEFMFETTY
                     NIPLIIKDPHSTRVNEVDDNLVYLHDLTSTVYDVANQPIPTAFQGESILPITRQSQNN
                     DRKGLLCQLAGHFVYFEQRMWRRKDYKLVFNATDRCELYDVRKDPEEMHNLFYDEAYQ
                     AVKKEMLEEMRQEMKRLNDPLENWVYRIIDEI"
     gene            complement(3717..5414)
                     /locus_tag="PXA33_002106"
     CDS             complement(3717..5414)
                     /locus_tag="PXA33_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HDL5121407.1"
                     /translation="MLLTIITFLVVTGLVGYISWLKTRNDDLSTSKGYFLAGRGLSAT
                     VIGCSMVLTSLSTEQLIGVNANSYAGNFSIIAWTVPAVIPLCFLALYLLPKYIRNGYT
                     TIPEFFENRFDRQTRLIMSGLFLVFYLFIVIPTALYTGAIAFNKIFNLETAFGLSYGA
                     AITYTVIAIGIVGAIYAIFGGLKAVAVSDTWNAVLLVIGALLVPVFALIYLGNGSMAE
                     GVKIITTTHVEKWNAVGSATDAVPWPTIFTGIMVVHFFYWTTNQAIVQRALGARDLKA
                     GQKGILIAALFLLLLPLILNLPGLLSFHILGEGLKPIDVSYPELVNKTFPTVLQGFFI
                     AALFGAILSTFNSFLNSAATIYCKDLLPSISKKQFTDAELISYAKKVSAVMAVITMIF
                     GPLLMFGTDGIFLLTKRFAGFVNIPIVALFVIGLFNKTVSGKAARIALLAHVILYFCI
                     VWVFDVKINFVYVQAGLFVFDVALMLILGIFLKRATPYEDNKINHANVDLTSWEYAPL
                     AVASIVLGLFTLYAFLSPLGLASPDGNPAMVLAVYAVLQAVVCVVFFAKCKGKKAVN"
     gene            complement(5558..6079)
                     /locus_tag="PXA33_002107"
     CDS             complement(5558..6079)
                     /locus_tag="PXA33_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDL5121408.1"
                     /translation="MSCQLSVMSDLEKGVVIYSQGYHAREFYFLKSGLIGLYHMLENG
                     KESLVRVYKANDYFGFRTFFGDNRYHCSARVLQPAELIRITPLNFIEFWSNNSALIIS
                     LLKQLSTELQDAEHRLSMVSYQKTQERVMNSIHYLHQRYPNYHWTNREIAEFAGCETE
                     TAIRLRRELKKIS"
BASE COUNT         1691 a         1363 c         1356 g         1677 t
ORIGIN      
        1 aaaattaaac aagctgttga aactattcaa ttacttcaat tagaagttga agaattaaaa
       61 ggtaaaaacg atgcagcgaa acaagaaaac gacaccttgc gcagcgaata tgaacaatta
      121 aaagtggaac aacaaaactt ccaagatcgt ttacgttcac ttttaggtca aatcgacaac
      181 gtttaattct attttcagca caaacggcta aatttttagc cgttttttat ttgttacagc
      241 tatggcaaaa ctctattttt actactcctc aatgaatgca ggcaaatcta ctactctgct
      301 tcaatcttct tataattacc aagaacgtgg aatgaacact ttagtttata ccgccgctat
      361 tgatgaccgt ttcggtgtgg ggaaagtcaa ctctcgtatc ggtatttcac aagaagccaa
      421 tttatttacc gctgatacca atctatttgc ggaaattaag gcattacact caaacaaagc
      481 gttacactgt atcttgattg atgaggctca gtttttaact aaaacacagg tttatcaatt
      541 aagtgatgtg gtcgataaac tcaaaattcc ggtgctttgc tacgggctac gcaccgattt
      601 ccaagctgaa ttatttgaag gtagcaaata tttgttggct tgggcagatg agctggaaga
      661 actcaaaacc atctgtgatt gtggcaaaaa agcccatttt gtcgttcgat taaatgaaaa
      721 aggcgaagcc attaaagacg gtgaacaaat acaaatcggc ggcaatgata gatatctctc
      781 tgtttgccgt tatcattaca aacaaaagtt aggtaagcta taagctacaa gaagcagcaa
      841 gcggttaaat tttcgggaat ttttgcaaat tttcagcgaa atttaaccgc ttgtttgtgt
      901 tatttacctc tctttacttt cctgcaaacc gaatttgctc actcatttgt ctcatcacac
      961 tcgaagtctc ggcaaaaaac tgttggtaag aggggcaaag gtagttgtgg ttttcgctgt
     1021 ttgcttgggg gacgatgcgg tgtttggggc agccgccgtg gcaaagtatg cggaatttgc
     1081 agtttaggca ggcttgcgag agttttgcct gtttattctg cccgaattgg cgttgggcgg
     1141 ctgatgagac gatctctgcc agctgctgtt ggttgaggtt gccgatgtgg taatcgggat
     1201 agacgaaatg gtcgcaactg tacacatcgc cacaggcttc caccaccagc gagctgccac
     1261 aggtcgggcg gaatacacag ttgttggagg gatagccaag ccattgcccg agcaggttat
     1321 caaactcgat cacaaaaatc tgcccacggt caggcagcca ttcgtgaaaa acatccacta
     1381 aaaattgccc gtagccttgg ggcggaacgg aaaagggttg taattccccc ttgtggcttt
     1441 ccaccaccgg aatgaactgc atataacggc taccgagggc tttgagggct tgataggttt
     1501 ctctgccccg ttgccagttg cggtcgttca ccaccgtgag ggtgttaaat tccacgccaa
     1561 cctctttcaa taaatcgacc gcacttttca cccgttcaaa ggtcggttta ccgttggtgg
     1621 aaatgcggta gcggtcgtga atgtcgctca gtccgtcaat ggataagccc accagaaaat
     1681 gattctcttt gaaaaagctc gcccactggc ggtttagggc aatgccgttg gtttggattg
     1741 cgttggtgat ctgcttgccg tttgcaaatt tttgttgata tttgaccgct tgtttgtaga
     1801 aatctaagcc cagcagggtc ggctcgcccc cctgccacac aaaatccacc cgttggcttt
     1861 ggttggcttc aatgtaatga cgaatgtagt tttgcagcgt ttcatcgttc ataaactgcg
     1921 atttttctgg cggaatggtc tgttccttgc tcagataaaa acagtactcg cactgaatat
     1981 tacagtgaaa actgctgggt tttgccatta ggtggaagta ggtggtggtc attgtatctc
     2041 taaattgtag gggcggactt tatgttcgcc cattgtttac attaattttc gggcgggtat
     2101 aaaacccgcc tctacaaaca attatatctc atcaataatc cgatacaccc aattttccag
     2161 tgggtcgttg aggcgtttca tttcctgtcg catttcttcc aacatctctt ttttcacggc
     2221 ttggtaggct tcgtcataaa agaggttgtg catctcttcg gggtcttttc tgacatcgta
     2281 gagttcgcag cggtcggtgg cgttgaagac caatttgtaa tccttacgcc gccacattcg
     2341 ctgttcaaaa tagacaaaat gccctgccag ttggcacaac aagcctttgc ggtcgttgtt
     2401 ttggctttgt ctggttatcg gcagaatact ctcgccttgg aaagcggtcg ggatcggttg
     2461 atttgccaca tcatacacgg tggaagtgag atcgtgcaaa taaacgaggt tgtcatcgac
     2521 ttcgttcacc cttgtagagt gcggatcttt aataatcagc ggaatgttgt aggtggtttc
     2581 aaacataaac tcgccttttt cgatcatctt atgcgccccc atcgcatcgc cgtggtcggc
     2641 ggtagcaacg aggaaggtat tttcatagag attgttctct tccaagaaac ggaacagctc
     2701 gccaatggca tcgtcaatca gcgtgatgta gccccagaat ttagaaatca cctctttcca
     2761 acgatcttcg ctcgcctccc acattcccca catttgtgaa atggtgcgga agtggttcgg
     2821 tttgccctca agcggtttga agaaactctc gtccaatacc acttggcttg ggtcatacat
     2881 tgaataatac ggctccggca ctacacacgg agtgtgcggc ccccagaagt tgatccacgc
     2941 aaagaacggt tttttctcat caagggaggc ttgaatgtat tttttcgcct cgtcaatgat
     3001 aaaccactct attgtctgct cgcgactgcc ggaaagcaaa ccacagagtt cctgcacacg
     3061 caggtgtgga ttgtcgccaa aataggctcg actgacttcg ggtgcgtcat agcccttttc
     3121 cgccagccat tcacgataac ggttggagtg tgttggcggc tgattaaaca cgaggttttt
     3181 atacacttgg ctgcccgggt agccatagcc gtcaaagtta tgaccggcga tgccataatc
     3241 ttcgggaacg gattttgtac caacgtgcca tttgccgatc acaaaggagt tatagccctc
     3301 cacgccggca atattagggt tttgttgtgg aatttcgcct gtgccgcctt tttcgccgtt
     3361 tttgataatc ccgtggctgg acggcattaa gccggtaaac agcgaggtgc gagccggccc
     3421 gcagaccgaa gccggcgtaa aggcgttgtt aaaccgaatg ccgtctttcg ccaagcgatc
     3481 caaattcggg gtttttacca cctgatgccc gtaagtgccg agcatatctt tacgcacttg
     3541 atctaacaaa atatagatga tgttattcat aatgacctct ttaaggcttg ttaataaccg
     3601 ataaattatc aagtttcgga gatctgttcc cctctttagc aaagagggga tttttgatct
     3661 cgagcctttt ccgttcttgg tatttttata ctaatgagga gaaggctcac attcatttaa
     3721 ttcaccgcct ttttgccttt acattttgca aaaaacacga cacaaaccac cgcttgtagc
     3781 acagcataca ccgccagcac cattgccgga ttgccgtctg gtgaggctaa accgagcgga
     3841 gagaggaaag cgtatagcgt gaataagccc aacacgatag aagccactgc cagtggggca
     3901 tattcccagc tggttaagtc cacattggcg tggttgattt tgttgtcttc gtatggagtc
     3961 gcacgtttta ggaaaattcc taaaatcagc attaacgcca catcgaacac gaacaagcca
     4021 gcttgcacat acacgaagtt gatttttaca tcaaacaccc aaacgatgca gaagtagaga
     4081 ataacgtgtg cgagtaaggc aatgcgtgcg gctttgcctg agacggtttt gttgaataag
     4141 ccgatgacaa acagtgccac aatcggaatg ttcacaaaac cggcgaaacg tttggtcagc
     4201 aagaaaatac cgtctgtgcc gaacatcagt aacggcccaa agatcatcgt aatcaccgcc
     4261 attaccgcag agactttttt cgcataactt atcagttccg catctgtgaa ctgttttttg
     4321 ctgatagacg gcagtaagtc tttacagtaa atggttgccg ctgagtttaa gaacgagttg
     4381 aaggtactca aaatcgcccc gaacagtgcg gcaataaaga agccttgtag cacggttggg
     4441 aaagttttat tcactaattc agggtaagac acatcaatcg gtttcaagcc ctcacctaaa
     4501 atatggaaac tgagtaagcc cggtaagttt aagataagcg gtagcaataa taagaacagt
     4561 gccgcaatta agatcccttt ttgaccggct tttaagtctc tcgcccccaa ggcacgttgc
     4621 acaatggctt ggttggtcgt ccagtagaag aaatgcacca ccataatacc ggtgaaaatg
     4681 gtcggccaag gcactgcatc ggtggcacta cccaccgcat tccatttttc aacgtgagta
     4741 gtggtgatga ttttcacccc ttctgccata ctgccattac ctaaatagat aagtgcaaac
     4801 accggcacta ataatgcccc aatgactaac aagaccgcat tccaagtgtc tgacaccgcc
     4861 accgctttta agccaccgaa aatggcataa atcgccccaa caataccaat cgcaatcacc
     4921 gtataggtaa tcgctgcccc atagcttaag ccgaaggcag tttcaaggtt gaagatttta
     4981 ttgaaggcaa tcgcaccggt gtaaagtgca gtcggaatga cgatgaaaag atagaacacc
     5041 aaaaacaagc ccgacataat caaacgggtt tggcggtcga aacggttttc aaagaactcc
     5101 ggaatggtgg tataaccgtt gcggatatat ttcggcaaca gataaagtgc caagaagcaa
     5161 agcggaatta ccgccggcac agtccacgca ataattgaga aattaccggc ataagagttg
     5221 gcattcacgc cgataagttg ctcggtagaa agggaggtta agaccatcga acagccgata
     5281 accgtagcac tcaagccacg ccctgccaag aaataacctt tagatgtcga aagatcgtcg
     5341 tttctggttt ttagccaaga gatatacccg actaatccag ttacaaccag aaaggtaatt
     5401 attgttaata acatattctt cacctcaata tgactatata aatgatatat cccattagag
     5461 acggcaatag cataaatctt atttttaacg atagaatatg atgtttgtca taatagatat
     5521 gtgatggtga tcacaactat gaataattta tgacaattta actgattttc tttaattctc
     5581 gccttaatcg aatggctgtt tcggtttcac aacctgcaaa ttcggcgatt tcacggttag
     5641 tccagtgata attgggataa cgttggtgta aataatgaat actgttcatt acccgctctt
     5701 gcgttttttg ataggaaacc attgataaac gatgctctgc gtcttgtaat tctgtcgaga
     5761 gctgttttaa cagagagata attaaggcgg aattattcga ccaaaactca atgaaattta
     5821 agggggtaat acgtatcaat tctgccggct gtaacacccg agccgagcaa tggtagcgat
     5881 tatcgccaaa aaaggttcta aacccaaaat aatcgttggc tttatatacc ctcaccaagc
     5941 tctctttacc attttcaagc atatggtaca gccctattaa tccgcttttt aaaaaataaa
     6001 attctctggc gtgataaccc tgagaataaa tgacgactcc tttttctaga tcagacatta
     6061 cacttaattg gcaagacatc aattgtt
//
LOCUS       DANLRK010000153         6064 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.153, whole genome shotgun sequence.
ACCESSION   DANLRK010000153 DANLRK010000000
VERSION     DANLRK010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6064
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.153"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(<1..195)
                     /locus_tag="PXA33_002108"
     CDS             complement(<1..195)
                     /locus_tag="PXA33_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121409.1"
                     /translation="MSTTLNEINITELDAGPEIKSLSLNDAVNSFLNDSACPICGKNC
                     QYWQRLKRSLNRKENQGKGDK"
     gene            complement(229..1515)
                     /gene="purD"
                     /locus_tag="PXA33_002109"
     CDS             complement(229..1515)
                     /gene="purD"
                     /locus_tag="PXA33_002109"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012771675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HDL5121410.1"
                     /translation="MNILIIGNGGREHALAWKVRQSPLADKVFVAPGNAGTAMENGIE
                     NVAISATDVPALVTFAKQNSVGLTIVGPEAPLVVGVVDAFRAHGLTIFGPTQAAAQLE
                     GSKAFTKDFLARHQIPTAEYQNFTEIEPALAYLKQKGAPIVIKADGLAAGKGVIVAMT
                     LQEAEDAVRDMLSGNAFGEAGSRVVIEEFLDGEEASFIVMVDGKNVEPMATSQDHKRV
                     GEGDSGLNTGGMGAYSPAPVVTKAIHQRVMDEIIYPTVQGMASENTPYTGFLYAGLMI
                     MPNGQPKVIEFNCRFGDPETQPIMMRLESDLVELCLKACEGKLDSVQSKWCEQAALGI
                     VLAAAGYPGDYRKGDEIIGIPTSTSNQKVFLAGVEQKEGKLLTNGGRVLCATALGNSV
                     FEAQQQALQLAEQIQWQGKFYRRDIGYRAVARKQAK"
     gene            1749..2123
                     /gene="rpsF"
                     /locus_tag="PXA33_002110"
     CDS             1749..2123
                     /gene="rpsF"
                     /locus_tag="PXA33_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011199651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HDL5121411.1"
                     /translation="MRHYEIVFMVHPDQSEQVPAMIERYTASVKEAGGQVHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQSVIDELETNFRYNDAVLRNLIVHTKAAVTEASPM
                     VKAKESKVAEAVAEVESEEAGE"
     gene            2098..2472
                     /gene="priB"
                     /locus_tag="PXA33_002111"
     CDS             2098..2472
                     /gene="priB"
                     /locus_tag="PXA33_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HDL5121412.1"
                     /translation="MNQRKQASNLPIDNCLILSGTVSSLVKQSLNPLGVPHYSFWLEH
                     RSIQTEVNLERQAWCKIQVVLAGNQFSYLTQQIKLGESVRIKGFIHTHKDFNGLNQLV
                     LHTEQIEFIDQEKPNGTLFPSS"
     gene            2447..2674
                     /gene="rpsR"
                     /locus_tag="PXA33_002112"
     CDS             2447..2674
                     /gene="rpsR"
                     /locus_tag="PXA33_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HDL5121413.1"
                     /translation="MARYFRRRKFCRFTAENVVEIDYKDIATLKNYISESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLALLPYTDNHQ"
     gene            2690..3139
                     /gene="rplI"
                     /locus_tag="PXA33_002113"
     CDS             2690..3139
                     /gene="rplI"
                     /locus_tag="PXA33_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HDL5121414.1"
                     /translation="MQVILLDKVAHLGSVGDQVTVKSGFARNFLIPQGKAVMATAANI
                     AHFEARRAELEAKAAAALATAQERAAKIAALAAVSVSATAGDDGRLFGSVSAKDIAEA
                     LTAAGVGVAKSEVRLGEGPLRTTGEHQVKIHLHPEVNATVAVNVVAE"
     gene            complement(3203..3979)
                     /gene="dnaQ"
                     /locus_tag="PXA33_002114"
     CDS             complement(3203..3979)
                     /gene="dnaQ"
                     /locus_tag="PXA33_002114"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HDL5121415.1"
                     /translation="MTEEKIIRQVVLDTETTGMNMAGGPPQIGHNIIEIGAVEVINRR
                     LTGRTYHVYIKPPREVDEEAIAVHGITNEFLQDKPVFAEIAEEFLDFIKGAELIIHNA
                     PFDVAFMDQEFSYLPNPPAKTAEMCTVTDSLQMARRMYPGKRNNLDALCDRLGIDNSK
                     RVLHGALLDAEILADVFLMMTGGQISLLGEEETEVVSEVIDIEEEFSAIVLNADEAII
                     LKANDEEEESHLALLKLIEKKSKGNCLWTKALTDTAEISH"
     gene            complement(4067..5737)
                     /gene="ettA"
                     /locus_tag="PXA33_002115"
     CDS             complement(4067..5737)
                     /gene="ettA"
                     /locus_tag="PXA33_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011608528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HDL5121416.1"
                     /translation="MSTQFVYTMHRVGKVVPPKRHILKDISLSFFPGAKIGVLGLNGA
                     GKSTLLRIMAGVDKEFEGEARPQPGIKIGYLPQEPKLAPQQTVREAVEEAVAEVKNAL
                     TQLDEVYAMYADENADFDKLAAKQAELEAIIQAHDGHNLQNQLERAADALRLPDWDAK
                     IEHLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDYEGTVVAITH
                     DRYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKEKRLAQEQAAESARQKSIEKELEW
                     VRQNPKGRQAKSKARMARFEELNSGEYQKRNETNELFIPPGPRLGDKVIEVQNLTKSY
                     GDRTLIDDLSFAIPKGAIVGIIGANGAGKSTLFRMLSGKEQPDSGSIVLGETVVMASV
                     DQFRDAMDDKKTVWEEVSNGQDILTIGNFEIPSRAYVGRFNFKGVDQQKRVGELSGGE
                     RGRLHLAKLLQRGGNVLLLDEPTNDLDVETLRALENAILEFPGCAMVISHDRWFLDRI
                     ATHILDYGDEGKVTFYEGNFSDYEEWKKKTFGEAATQPHRVKYKRIAK"
BASE COUNT         1592 a         1400 c         1278 g         1794 t
ORIGIN      
        1 tttatctccc ttaccttgat tttcttttct atttaaagat ctttttagtc tttgccaata
       61 ttgacagttt tttccacata tagggcacgc agagtcattt agaaaagagt ttactgcatc
      121 gtttaatgat agtgatttaa tttcggggcc tgcatcgagt tcagtgatat taatttcatt
      181 aagtgttgta ctcataataa atttttcctt gttttaagag tttataaatt atttcgcttg
      241 ttttcttgcc actgctcggt agccaatatc tcggcgatag aatttgcctt gccattgaat
      301 ttgttcggca agttgtaagg cttgttgttg agcttcaaat acactgttgc caagtgcggt
      361 ggcacaaagt acacgtccac cgttggtgag cagttttcct tctttttgct caacccctgc
      421 taagaaaact ttttgattgg aggtagaggt cggaatgcct atgatttcat cgcctttacg
      481 gtaatcgccc gggtagcctg cggcagctaa aacaatgcct aatgcggctt gttcacacca
      541 ttttgattgc acgctatcca atttgccttc gcacgctttt aagcacaatt caacgaggtc
      601 tgattccaaa cgcatcataa tcggctgagt ttcagggtct ccaaaacggc agttaaattc
      661 aatcactttc ggctgaccgt ttggcataat cattaagccg gcatagagaa aaccggtgta
      721 aggggtattt tcgcttgcca ttccttgcac ggtcgggtaa ataatttcgt ccattacacg
      781 ttggtggatt gcctttgtta ccaccggagc aggggagtaa gcccccattc cgccggtatt
      841 taagccgcta tcgccctcgc caacacgttt gtggtcttgt gaggttgcca ttggttcgac
      901 atttttgccg tccaccatca caataaaact ggcctcttct ccgtctaaaa attcctcgat
      961 caccacacgg ctgccggctt caccaaaggc gttgcctgac agcatatcac gcactgcatc
     1021 ttccgcctct tgtaaagtca ttgcaacaat tacgcctttg cctgcggcta aaccgtctgc
     1081 tttgattaca atcggtgcac ctttttgttt taagtaggca agtgccggtt caatttcggt
     1141 gaaattttgg tattctgcgg ttggaatctg atggcgagcc aagaaatctt tggtaaatgc
     1201 tttagaacct tctaattgag cagcggcttg ggtcgggccg aaaatggtta aaccgtgggc
     1261 tcgaaaagca tcaaccacgc caactactaa cggtgcttca gggccgacaa tcgtcaaacc
     1321 tacgctattt tgtttagcga atgtaactaa ggcaggcacg tcagtggcgg aaatagctac
     1381 attttcaatg ccgttttcca tcgctgtgcc ggcattgccg ggggcgacaa agactttgtc
     1441 tgcgagcggc gattgacgaa ctttccaagc aagagcgtgt tcacgcccac cgttgccgat
     1501 aattaaaata ttcattggga ttccttgcaa aaatgataaa gcaaacgttt gcgtattgta
     1561 cttgatttgt ttatctgctg acaagcagtc gtttttctcg gattttttgc aaaatacgaa
     1621 attgttgctt gcaaaatagg cttttttcag tataatcctg ccgttcagtc acattcgttt
     1681 tttaatcctt gcttccacaa gttggtgact ggctaaagtt ggaggctgct aacccgtaag
     1741 gatagtcaat gcgtcactac gaaatcgttt ttatggttca cccggaccaa agcgaacaag
     1801 taccagcaat gattgagcgc tacacagcgt ctgtaaaaga agctggcggt caagttcatc
     1861 gcttagaaga ttggggtcgt cgtcaattag catacccaat caacaaactt cacaaagcac
     1921 actatgtgtt aatgaatgta gaagcacctc aaagtgtaat cgacgagtta gaaactaact
     1981 tccgttataa cgatgcagtt cttcgtaact taatcgttca cactaaagcg gcagtaactg
     2041 aagcgtcacc aatggtgaaa gcgaaagaaa gcaaagttgc tgaagcggta gctgaagttg
     2101 aatcagagga agcaggcgag taatttgccg attgataatt gtttaatctt aagcggaacg
     2161 gtttccagct tggttaaaca aagcctaaac ccccttggtg tgcctcatta cagtttttgg
     2221 ttagaacatc gttcaatcca aaccgaagtg aatttagaac gccaagcgtg gtgcaaaatc
     2281 caagtggttt tggcaggcaa tcaatttagt tatttaaccc aacaaattaa gctcggtgag
     2341 agcgtgagaa ttaaaggttt tatccatact cacaaagact ttaacggttt aaatcagtta
     2401 gtcttacaca ccgaacaaat tgaatttata gatcaggaga agccaaatgg cacgttattt
     2461 ccgtcgtcgt aagttctgcc gtttcacagc ggaaaatgtt gttgaaatcg attacaaaga
     2521 tatcgctaca ttaaagaact acatttctga aagcggcaag attgttccta gccgtattac
     2581 tggtactcgt gcgaagtatc aacgtcaatt agctcgtgca attaaacgcg ctcgctacct
     2641 tgcgttactt ccatatactg ataaccacca ataatcagga gagagtacaa tgcaagttat
     2701 tttattagac aaagttgctc acttaggttc tgtgggcgac caagtaaccg ttaaatctgg
     2761 ttttgctcgt aacttcttaa tcccacaagg taaagcggtt atggcaactg cggcaaacat
     2821 tgctcacttt gaagcacgtc gtgcagaatt agaagcaaaa gctgcagcag cattagcaac
     2881 tgcacaagaa cgtgcagcga aaattgcagc attagcagca gtatcagtat ctgcaactgc
     2941 aggtgatgat ggtcgtttat tcggttctgt atcagctaaa gacatcgctg aagcattaac
     3001 tgcagcaggt gttggagttg ctaaatctga agttcgttta ggtgaaggtc cacttcgtac
     3061 aaccggcgaa caccaagtta aaattcactt acaccctgaa gtgaatgcaa ctgtagcagt
     3121 aaacgttgtt gctgaataat cagtagaaat agaaaaccca gcgaaagctg ggttttttgt
     3181 ttgctttatt tattggttag aatcagtgag atatttctgc tgtgtcggtt aatgcttttg
     3241 tccataggca atttcctttg gattttttct caatcagttt cagaagtgct aaatgcgact
     3301 cttcctcttc atcgtttgct ttaagaataa ttgcctcatc ggcatttaac acaattgcag
     3361 aaaattcttc ttcaatatca ataacttcac ttaccacttc agtttcttct tcgcctaata
     3421 atgaaatctg accgccggtc atcattagga atacatcagc taagatctcc gcatccagta
     3481 acgccccgtg tagtacacgc ttgctattat caatgccgag cctgtcgcaa agtgcatcta
     3541 aattattccg tttgccgggg tacattcttc gtgccatttg caggctatcg gttacggtac
     3601 acatttctgc ggtttttgcc ggtggattag gcagataaga aaattcttga tccataaacg
     3661 ccacatcgaa gggggcattg tgaataatta attccgcacc tttgatgaaa tccaaaaact
     3721 cctcggcaat ttcagcaaaa acaggtttat cttgcaaaaa ttcattggtg atgccgtgaa
     3781 ctgcaatcgc ctcttcatct acctcacgcg gtggcttgat ataaacgtga taagttctgc
     3841 cggttaaacg gcggttaatg acttcaaccg caccgatctc aataatatta tgcccaatct
     3901 gtggcgggcc acctgccata ttcattcctg tggtttcagt atccagcact acttggcgga
     3961 taattttttc ttctgtcata ttcaatttaa aattgtaggg gtgaaatatt tttatccctt
     4021 aattctgcat ttgattaggg gcgaaacata tttcgcccct acattgttac ttcgcaatcc
     4081 gtttatattt cactcggtgc ggttgggtgg cggcttcacc gaaggttttc ttcttccact
     4141 cttcatagtc ggagaagtta ccctcgtaga acgtcacttt gccttcatcg ccgtagtcta
     4201 aaatatgggt ggcgatacgg tctaagaacc agcggtcgtg cgaaattacc attgcacagc
     4261 ccgggaactc taagatcgca ttttccaacg cacgcagggt ttctacgtct aagtcgttgg
     4321 tcggctcgtc cagtaacaac acgttaccgc cacgttgtag tagcttcgcc aagtgtaaac
     4381 gaccacgttc accgcctgaa agctcgccca cacgtttttg ctgatctacg cctttgaagt
     4441 tgaaacgccc cacataagca cggcttggga tttcaaagtt accaatcgtg agaatatcct
     4501 gcccgttcga cacctcttcc cacacggttt tcttatcgtc catcgcatca cggaactgat
     4561 cgaccgatgc cattaccacc gtttcaccaa gcacgattga accagaatcc ggctgttctt
     4621 taccagaaag catacggaac aaggttgatt tacccgcacc atttgccccg ataatcccga
     4681 caatcgcccc cttcggaata gcgaacgaca agtcgtcaat caaagtgcga tcgccgtaag
     4741 atttagtcag gttttgtacc tcgatcactt tatcacctaa gcgtggaccc ggtggaataa
     4801 agagttcgtt agtctcgtta cgtttttgat attcgcctga gttaagctct tcaaagcgtg
     4861 ccatacgagc cttacttttc gcttggcgac cttttggatt ttggcgtacc cattccaact
     4921 ctttctcaat cgatttttga cgagccgatt ccgccgcttg ctcttgtgcc aagcgttttt
     4981 ctttctgctc caaccaagaa gagtagttac cttcccaagg aatgccttca ccacggtcaa
     5041 gctctaagat ccagcccgca acgttgtcta agaagtaacg gtcgtgggta attgccacta
     5101 cggtgccttc gtagtcgtgg aggaaacgct ccaaccacgc caccgattcc gcatctaagt
     5161 ggttggtcgg ctcatctaac aataacatat ccggtttttc cagcaacaga cggcaaagtg
     5221 ccacacggcg gcgttcaccc cctgataaat gctcgatttt tgcgtcccaa tccggcagac
     5281 gtaacgcatc ggcagcacgc tctaactggt tttgaaggtt atggccatcg tgggcttgaa
     5341 taatcgcctc taattccgcc tgttttgccg ctaatttgtc gaaatcggca ttctcatcag
     5401 catacatcgc atagacttca tcaagctggg ttaaggcgtt tttcacttcc gcaaccgctt
     5461 cttccaccgc ttcacgcacg gtttgctgtg gggcaagttt cggttcttgt ggaagatagc
     5521 cgattttaat tcccggttgt ggacgagcct cgccctcgaa ctctttatct acgcccgcca
     5581 taatgcgtaa aagcgttgat ttacccgccc cgtttaagcc gagcaccccg attttcgccc
     5641 ccgggaaaaa gctcagggaa atatctttaa gaatgtgacg cttcggcggc acgactttgc
     5701 cgacacggtg catcgtatat acaaattgag ttgacattgg ttgtattcct cacaaaaaaa
     5761 ttcgccttat tttacttgaa tttacagcca aaatataggg tggttttggc ttttggtaaa
     5821 gtaacctttt ccaatagaga tgttatttga ggctgcatag tactatgcat ttctcttacc
     5881 ttgacaggta agagaaacaa atcctgaagg aaaaaaattt taaggaagtt attctatgca
     5941 atatgtatgt ccaaaaacac tcaaattatt caacagcatt gttggttgtt ataagggaga
     6001 agattaattc tctacctcat caaggcttga ttttagattt aatatccatt tctaaagatg
     6061 attt
//
LOCUS       DANLRK010000154         6053 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.154, whole genome shotgun sequence.
ACCESSION   DANLRK010000154 DANLRK010000000
VERSION     DANLRK010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6053
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.154"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            86..1564
                     /gene="fruB"
                     /locus_tag="PXA33_002116"
     CDS             86..1564
                     /gene="fruB"
                     /locus_tag="PXA33_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HDL5121417.1"
                     /translation="MLNLSAKNIRLNGSASNKEEVIRFVAAELVAKGNVAAGYEVGML
                     NRETQTSTFLGNGIAIPHGTLDTRDLVKETGVQIIQLPQGVEWGEGNTAYVVIGIAAK
                     SDEHLTLLRQLTTVLSDEEAAATLAKTQNLDEFIAVLSGKKNLPALSQELISLNIESS
                     SPITLSAINAAKLQEQGYVSQAFISDVVANKALHLGNNLFVVDSAKGNQANGIAIARN
                     TQGQTLLTVAETDNALQPLLTQLLKPEVRSQFAHFSAEQMLGLLKGESVASTPVTNVE
                     ASGNQVVGTFTIRNDNGLHARPAAVLVQTVKPFSSKISVENLDRNTAPASAKSAMKVV
                     ALGASQGHRLRFVAEGEDAQQAIEALQQAIINGLGESVSFVPAVADTIESIAVSAIPA
                     EETATAVDANTKEATFVIKNEHGLHARPSAVLVNEVKKFNAKIEVQNVDKNSPLVSAK
                     SLMKIVALGVTKGTTLRFVASGDDAEVALAAIGAAIEAGLGE"
     gene            1578..2522
                     /gene="fruK"
                     /locus_tag="PXA33_002117"
     CDS             1578..2522
                     /gene="fruK"
                     /locus_tag="PXA33_002117"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDL5121418.1"
                     /translation="MRIATVTLNPAFDLVGKLQRIEIGEVNTVETLGLYPAGKGINVA
                     KVLADLGTKLSVTGFLGEENQGDFVNAFKHNDLADEFYRINGKTRINVKITETEADVT
                     DLNFLGFEISESDWEKFVTLSHQWESKFDLVAVCGSLPRGVTPEKFASWLESLHRQGL
                     KVVLDTSNAALTAGLTAHPWLVKPNRRELEVLVGHSLNSIEEIISAAKQLRSRGIENV
                     IISMGEQGSLWLNHEGVLQARPPRCENVVSTVGAGDSMVASLIYGLSQGWSKEQTLRF
                     ASATSALAVVQSNVGIADRAALDDIIKQTDITVISENH"
     gene            2548..4212
                     /locus_tag="PXA33_002118"
     CDS             2548..4212
                     /locus_tag="PXA33_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL5121419.1"
                     /translation="MNISFVFPQTLGNARRFLVNEMLSAAAKQQGHNVVAANQADFVV
                     LFDNAIPQEAAGKQGAILDLEQAFAQPEASLKQAVENAQTFANATATTAVSAVSSGVK
                     NIVAVTACPTGVAHTFMSAEAIENYAKAQGWNIKVETRGQVGAGNPISAEEVAAADLV
                     FVAADIDVDLEKFKGKPMYRTSTGLALKKTTQEFEKAFAQAKIYEGGVAKADNAEAPT
                     SEKKGLYKHLMTGVSHMLPLVVAGGLLIAISFMFGIEAFKDETIAGGLPKALMDIGGG
                     AAFHLMIAVFAGYVAFSIADRPGLAVGLIGGMLATTAGAGILGGIVAGFLAGYTVKFL
                     NGAIQLPASLTSLKPILILPLLGSAIVGLLMIYVINPPVKALMDALVEWLNTMGQTNA
                     IILGIILGAMMCTDMGGPVNKAAYTFGVGLIASQQYMPMAAVMAAGMVPPIGMAIATL
                     IARSKFNTNQRDAGKASFVLGLCFISEGALPFVAADPVRVIASSILGGATAGAISMAL
                     GITLQAPHGGLFVIPFVSQPLMYLAAIAIGSLVTGVVYAAIKQKAE"
     gene            4379..4897
                     /locus_tag="PXA33_002119"
     CDS             4379..4897
                     /locus_tag="PXA33_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lhr family helicase"
                     /protein_id="HDL5121420.1"
                     /translation="MILLRGDTMQFIEFTDPDSGISYQYSEFTIANVAFIINFCSDAD
                     VISTLSALGKDITNYINTYSCCTIKFMAKEHLENSGSNIDIYAPAANHQFKRKEIIAL
                     QETLERLLFEHYVRFTPESYLFIAERDSLNRMYQRMCVPRCDFMQSFQVVYPLGVNQD
                     CFILITPKGNLK"
     gene            complement(5050..5913)
                     /gene="rarD"
                     /locus_tag="PXA33_002120"
     CDS             complement(5050..5913)
                     /gene="rarD"
                     /locus_tag="PXA33_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HDL5121421.1"
                     /translation="MLKGILASLAANVLFGAVYYFAILLRPLEGEAMFGFRIVVVIPF
                     ILLAIILFKQQSAFADLLQKIRQNPPLVLVILLLALNTGVQLWLFLWAPNHGQALQVS
                     FGYLLLPIVAVALGKMVFKEHFTRLKWLSLLFAVIGVGSSLFFNSTISWATWVAGLGY
                     PLYITLRRYFQINTLATFFVELLLVLPVALYYLSQVEMTPIVAENPQIYFYLGLLGLV
                     SGSAFILYILSSNLLPINVLGLLGYVEPLVMLLISFAIGEKLDANSYILMVCLLVSIG
                     LLSWDSFRSKV"
BASE COUNT         1856 a         1324 c         1281 g         1592 t
ORIGIN      
        1 ttggctataa cggtttagca aaatatagag taaactaacc ataacattac agtttaaaaa
       61 tcaactttaa atccaaggag ctaaaatgct taatttatcg gcaaaaaaca tccgtttaaa
      121 tggttcagca agcaacaaag aagaagttat ccgttttgtt gccgccgaat tagtcgccaa
      181 gggcaatgta gcagccggct atgaagtcgg tatgcttaat cgtgaaaccc aaacctctac
      241 ttttttaggt aacggaattg ctattcccca cggcacgctt gatacccgtg atttagtcaa
      301 agaaaccggc gtgcaaatta tccaacttcc gcaaggcgtg gaatggggcg aaggcaacac
      361 tgcttatgta gttatcggga ttgcggctaa atcggacgaa cacttaacct tactccgtca
      421 actcaccact gtattaagtg atgaagaggc tgctgccaca ttagcgaaaa cgcaaaattt
      481 agatgaattt attgccgtat taagcggtaa gaaaaacctt cctgccttat ctcaagagct
      541 gatttctctg aatatcgaat cttcaagccc tatcacttta tcagccatta acgccgcaaa
      601 actacaagag caaggctatg tttcccaagc attcatcagc gatgtggttg ccaacaaagc
      661 gttacattta ggcaataatc tatttgtggt ggattccgcc aaaggcaacc aagctaacgg
      721 tatcgctatt gcccgtaata cgcaaggtca aaccctacta actgtggcag aaaccgacaa
      781 tgctctacag ccactattaa ctcaattatt aaaaccagaa gtaagaagcc agtttgctca
      841 tttctctgcg gagcaaatgc ttggtttatt aaaaggcgaa agcgtagctt caacccctgt
      901 aaccaatgtc gaagcgagtg gcaaccaagt ggttggcaca ttcaccattc gtaacgacaa
      961 cggcttacac gcccgcccgg ctgcggtgtt agtacaaacg gtgaaaccgt tctcctcaaa
     1021 aatcagcgtt gaaaatttag accgcaacac tgcaccggca agtgcgaaaa gtgcaatgaa
     1081 agtagtagca ctaggggcaa gccaagggca tcgtttacgc tttgtggcag agggagaaga
     1141 cgcccaacaa gcgattgagg cattacaaca agcaattatt aacggcttag gcgaaagtgt
     1201 ttcattcgta ccggcggtgg cagatacgat tgaaagcatt gctgtttcag caattcctgc
     1261 ggaagaaact gcgactgctg tcgatgccaa tacgaaagaa gcaacttttg taattaaaaa
     1321 cgaacacggt ttacacgctc gcccaagtgc ggtgttggtg aatgaagtga aaaaattcaa
     1381 cgccaaaatt gaagtgcaaa atgttgataa aaattcaccg cttgtaagtg ccaaaagctt
     1441 aatgaaaatt gttgcccttg gcgtaaccaa aggcacgacg ctacgctttg tggcaagcgg
     1501 tgatgatgcc gaagtagcac ttgcggcaat cggtgctgcg attgaagcag gtttaggcga
     1561 ataagcgagg aatcattatg cgtattgcaa ccgttaccct caaccctgcc tttgatttag
     1621 tcggcaaact tcaacgtatt gaaatcggcg aagtgaacac cgtagaaacc cttggtttat
     1681 accctgccgg caaaggtatt aacgttgcta aagttttagc tgacttaggc acaaaacttt
     1741 cggttaccgg ctttttaggc gaagaaaatc aaggcgattt cgtaaatgcc tttaaacaca
     1801 atgatttggc tgatgaattt taccgtatta acggtaaaac ccgcattaat gtcaaaatca
     1861 ctgaaaccga ggctgatgta actgatctga actttctcgg ctttgaaatt tcggaaagcg
     1921 attgggaaaa attcgtcaca ttatctcatc agtgggaaag caaatttgac ttagttgcag
     1981 tgtgtggatc tctccctcgt ggcgtaacgc cggaaaaatt tgcttcttgg ttggaatctt
     2041 tacaccgcca aggcttaaaa gtggtgttag ataccagcaa tgcggctctc accgccggct
     2101 taaccgctca cccttggtta gtaaaaccaa accgccgaga gctggaagtg ctggtcgggc
     2161 attcactcaa ctcaatcgaa gagattatta gtgcagctaa acaattacgt tcccgtggta
     2221 ttgaaaatgt aattatatca atgggcgaac aaggctctct ttggctaaat cacgaaggcg
     2281 tgttacaagc ccgcccacca cgttgcgaaa atgtagttag cactgtaggt gctggtgatt
     2341 caatggttgc cagcttgatc tacggcttat ctcaaggttg gagcaaagag cagaccttgc
     2401 gttttgcctc agctacttct gctctagctg ttgttcaaag taacgttggg attgcagacc
     2461 gtgcggcgtt agacgatatt attaaacaaa ccgacattac cgtgatttca gaaaatcatt
     2521 aaacctcaat caaaggaaca aaccaatatg aatatttctt ttgtatttcc tcaaacacta
     2581 ggcaacgcac gccgtttctt agtgaatgaa atgttatctg ccgctgccaa gcaacaagga
     2641 cacaatgttg tggcggcaaa ccaagctgat tttgtggtgt tattcgataa cgcaattcca
     2701 caagaagctg ccggcaagca aggggcaatt ttggacttag aacaagcctt tgctcaacct
     2761 gaagccagtc tcaaacaagc ggtcgaaaat gcacaaactt ttgcaaatgc taccgcaacc
     2821 actgcggttt cagcggtttc atctggtgtg aaaaatatcg ttgctgttac cgcttgccca
     2881 actggcgtgg ctcatacttt tatgtctgcg gaagcaatcg aaaattatgc caaagcacag
     2941 ggttggaaca tcaaggttga aacccgtggt caagtaggag caggaaatcc aatttccgcc
     3001 gaagaagtcg cagcggcaga tttagtcttt gtagctgccg atattgatgt cgatttggaa
     3061 aaattcaaag gcaagccgat gtatcgcacc tcaaccggtt tagcattgaa gaaaaccact
     3121 caagaatttg aaaaagcctt tgctcaagcg aaaatttacg aaggtggtgt ggcaaaagcg
     3181 gataatgctg aagctccaac ttctgagaaa aaagggttat ataaacactt gatgaccggt
     3241 gtatcacata tgttaccgct ggtggtagca ggtggcttgc tcatcgctat ttcctttatg
     3301 ttcggaattg aagcctttaa agatgaaacc atcgcaggcg gcttgccgaa agccttaatg
     3361 gatattggtg gcggtgcggc attccactta atgattgcgg tattcgcagg ctatgtagcg
     3421 ttctcaattg cagaccgtcc ggggttagct gtcggtctta tcggcggtat gcttgctacc
     3481 accgcaggag cagggatttt aggtggtatc gtagccggtt tcttagccgg ttataccgta
     3541 aaattcttaa atggtgcgat tcagctaccg gcaagtttaa cctcacttaa acctattttg
     3601 attttaccac tcttaggctc agcgattgtc ggcttactga tgatttatgt catcaaccca
     3661 ccggtgaaag cattaatgga tgccttagtg gaatggttaa acacaatggg gcaaaccaat
     3721 gcaattattt taggtattat tctcggtgcg atgatgtgta ccgatatggg tggtccggtg
     3781 aataaagcgg cttatacctt tggtgtgggt ttaattgctt cacaacaata tatgccaatg
     3841 gcagcggtga tggcggcagg tatggttcca ccaattggta tggcgattgc gaccctgatt
     3901 gctcgtagca aattcaacac caaccaacgt gatgcaggta aagcttcatt cgtgttaggt
     3961 ttatgcttta tttcagaagg ggcattaccg ttcgtggcgg cagaccctgt gcgtgtaatt
     4021 gccagctcaa tcttaggcgg tgcaaccgcc ggtgcaattt caatggcact aggcattacc
     4081 ctacaagccc cacacggcgg tttattcgtg attccattcg tttcacaacc gttaatgtac
     4141 ttagctgcta ttgccatcgg ctcattagtc accggcgtag tctatgccgc tatcaaacaa
     4201 aaagcagaat aaagcacaaa cacaaataca aacaaaaacg gagcgaaacg ctccgttttt
     4261 atatcatatt ggtacttact gaattctatt tcatcgaaat atcaagtcta aaatattatt
     4321 tttgcgatcg agatcgaaaa aatcattttc atcactttac gaataatatc aactcgttat
     4381 gattttgcta cgaggagata ctatgcagtt tattgaattt accgatcctg atagtggaat
     4441 ttcttatcaa tacagtgaat ttactattgc taatgtcgca tttatcatta atttctgctc
     4501 agatgccgat gttatatcga ctttgtccgc attaggcaaa gatatcacaa actatataaa
     4561 cacctattcc tgttgcacaa tcaaatttat ggcgaaagag catctagaaa atagcggttc
     4621 taatattgac atttatgccc ccgcagcgaa tcatcagttc aaacgtaaag aaataattgc
     4681 tttacaagaa acccttgagc gtttattatt tgagcattat gtacgtttta caccagaaag
     4741 ctatctcttc attgccgaga gagattcact taaccgaatg tatcagcgta tgtgtgtgcc
     4801 acgatgtgat tttatgcaat ctttccaagt ggtttatccc ttgggcgtta atcaagattg
     4861 tttcatttta attacgccaa aaggaaatct aaaatgaatc aacaacgtcc agtttccgtt
     4921 cgtttaaagc aagaaggctt aatcgctttt aagcaagcct atcagcgagc aaaaaaagca
     4981 ggtctattga aaccggtcaa ataatcttta aaccgcttgc tctacaagcg atttattttt
     5041 ccttaattat cacaccttgc tacgaaaact atcccaactc aataatccaa tcgaaaccag
     5101 caaacagacc atcaaaatat aggaattggc atcgagtttt tcgccgatgg caaaagaaat
     5161 cagcaacatc accaacggtt caacatagcc cagtaaacct aatacattaa tcggtaataa
     5221 attgctcgag aggatataaa gaataaaagc actgccactt accagcccca gcaatccgag
     5281 gtagaaataa atttgcggat tttctgccac tattggcgtc atttccactt ggctgagata
     5341 ataaagtgca acgggtaata ccagtaacaa ctccacaaaa aaagtagcta acgtgttgat
     5401 ttgaaaataa cggcgaaggg taatataaag cggataaccc aacccagcta cccaagtcgc
     5461 ccacgaaatc gtgctgttaa aaaacaaact actgccaaca ccgatgactg cgaaaagtag
     5521 agaaagccat ttcaggcggg taaaatgttc tttaaacacc atttttccta gagccaccgc
     5581 cacaatcggt aaaagcaagt aaccaaacga cacctgcaac gcctgcccgt gattaggagc
     5641 ccataaaaac agccaaagct gcacgccggt gttgagagca aggagcaaaa tgaccaatac
     5701 aagcggtgga ttttgtcgaa ttttttgcaa aagatccgca aaagccgatt gctgtttaaa
     5761 caatataatc gccagcaaaa taaacggaat gactaccact atccgaaagc cgaacatcgc
     5821 ctctccctct aacgggcgaa gcaatatcgc aaaatagtaa actgctccaa acagcacatt
     5881 ggctgcaagc gaagctaaaa ttcctttgag catttggctt tcctcttatc aacaatcaaa
     5941 caacaagcgg ttgactttga acaaaaattt acaaatctta tctacgcccc atagtatgaa
     6001 aaaatttgta agtaagctag ctatattcga tttttcccga tacaatgctc cca
//
LOCUS       DANLRK010000155         6006 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.155, whole genome shotgun sequence.
ACCESSION   DANLRK010000155 DANLRK010000000
VERSION     DANLRK010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6006
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.155"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            20..1009
                     /gene="dusB"
                     /locus_tag="PXA33_002121"
     CDS             20..1009
                     /gene="dusB"
                     /locus_tag="PXA33_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HDL5121422.1"
                     /translation="MQIGQYAIKNRIFLAPMAGITDQPFRRLCSQLGAGLTFSEMMST
                     NPDVWHTEKSRLRLAHHDEIGLNAVQIAGSDPVEMAKAAKINVEYGADIIDINMGCPA
                     KKVNKNMAGSALLRNPKLVAQILDSVVNAVDVPVTLKIRTGWDQENRNCLQIAKIAEQ
                     AGIKALTIHGRTRSCLFNGEAEYESIKAVKEAISIPVIANGDIDSAEKAKFVLDYTKA
                     DAIMIGRASFGNPWIFKEINDFFETGLSSPLSIDKKYQLMFKHIEDLHQFYGEEKGYR
                     IARKHVGWYTEKLNPESNFKRTFNTLSSTKEQLYALEDFVKSIHLESQLRKVG"
     gene            1014..1310
                     /gene="fis"
                     /locus_tag="PXA33_002122"
     CDS             1014..1310
                     /gene="fis"
                     /locus_tag="PXA33_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011199710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HDL5121423.1"
                     /translation="MLEQQPTQNPLTVTMLNAQAQQVNKPLRDNVKAALKNYLSQLNG
                     EDPTELYELVLSEIEHPMLDMVMQYTRGNQTRAATMLGINRGTLRKKLKKYGMG"
     gene            complement(1415..4201)
                     /locus_tag="PXA33_002123"
     CDS             complement(1415..4201)
                     /locus_tag="PXA33_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoferrin/transferrin family TonB-dependent
                     receptor"
                     /protein_id="HDL5121424.1"
                     /translation="MKYHHFRYSPVALTVLFALSHSYGAATENKKIEENNDLAVLDEV
                     IVTESHYAHERQNEVTGLGKVVKNYHEMSKNQILGIRDLTRYDPGISVVEQGRGASSG
                     YAIRGVDKNRVSLLVDGLPQAQHYHTLGSDANGGAINEIEYENIRSIELSKGASSAEY
                     GSGALGGAIGFRTKDAQDIIKEGQHWGLDSKTSYASKNSHFLQSIAAAGEAGGFEALV
                     IATHRHGKETKIHSEANKLKHNIRRITGFENRYDLYPNSAQNAPGGSFFIVEDTCPTL
                     DCTPRARVKLNRDNFPVRTFPEYTPEERKQLEQIPYRTEQLSAQEYTGKDRIAPNPLD
                     YKSNSVFMKFGYHFNSSHYLGAILEDTKQRYDIRDMQTPAYYTKDDINLSLRNYVYEG
                     DNILDGLVFKPRIPYGLRYSHVKFFDERHHKRRLGFTYKYKPENNRWLDSIKLSADKQ
                     DIELYSRLHRLHCSDYPVVDKNCRPTLDKSWSMYRTERNNYQEKHRVIHLEFDKALNA
                     GQGVFKQTHKLNLGLGFDRFNSLMDHGDMTAQYTKGGYTSYRGRGRLDNPYIYRRDPR
                     SIETVSLCNNTRGDILNCEPRKIKGDSHFVSFRDLVISEYVDLGLGVRFDQHRFKSDD
                     PWTLSRTYRNWSWNGGITLKPTEFVSLSYRISNGFRVPAFYELYGKRDHIGLKDNEYV
                     QRAQRSHQLEPEKSTNHEIGVSFKGQFGYLDVSYFRNNYKNMIATACKRIIQKSHCFY
                     NYHNIQDVALNGINLVAKFDLHGILSMLPDGFYSSVAYNRVKVKERKLTDSRLDSVND
                     PILDAIQPARYVLGFGYDHPEEKWGIGITTTYSKAKNADEVAGTRHHGIHRVDLGGKL
                     TGSWYTHDITGYINYKNYTLRGGIYNVTNRKYSTWESVRQSGVNAVNQDRGSNYTRFA
                     APGRNFSLAFEMKF"
     gene            complement(4295..>6006)
                     /locus_tag="PXA33_002124"
     CDS             complement(4295..>6006)
                     /locus_tag="PXA33_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HDL5121425.1"
                     /translation="LLAACSSNGGSFDVQSAKVESQTQTTPKKPSLQDDNSNARRTVS
                     ASETEALLQPGFGFSAKIPRRNLFPQGKEDVAPIGDIKEITGDLTKIPYEEEVKAYGS
                     SADGFSHTHDRNHKLYTRDFNFVRSGYVLHSGAKPEIKPKEILRTGAHGYVYYLGIEP
                     AKAIPTQKATYKGYWDFTTDARKGRDSKYFSNSAGINIGATPENSYDINVDDSEKPMG
                     HTGEFTADFANKTLTGTLVRNGYVSRSKEQKITTIYDIDAKIKGNRFSGKANAKNTDD
                     PYFGKSSTTLEGGFFGGKAQELAGKFLADDKSVFVVFAGKRDAKKDDSESAFDAFKIK
                     LKDLNKSEMDTFGNATHLIINNKQIPLIAEGKKSFAEMKFDDLVTRTIDDKTYRVSVC
                     CNNLDYVKFGTYSEGNNSDTAHQYLVGERTAVADLPTGTVKYRGTWDGVMYSKSGSAG
                     AESPSNSESGTRSLFDVDFVNKKINGKLIANDGVEERPMLTLEGNLKGNGFAGTAKTG
                     NSGFNLDPKSTNGGTVVHINTQFEGGFYGPKATELGGIVHSAETDKDRVSITFGGKRQ
                     IEK"
BASE COUNT         1820 a         1256 c         1071 g         1859 t
ORIGIN      
        1 tgttaataaa aggttaaaaa tgcaaatagg acaatatgca atcaaaaatc gtattttcct
       61 tgcacctatg gctggaatca cagatcagcc ttttcgccgc ttatgtagcc aacttggagc
      121 aggtttaaca ttttcagaga tgatgtctac caatcctgat gtatggcata ctgagaaatc
      181 acgattgcgg ttagcacacc acgatgaaat aggcttaaat gccgtacaaa ttgcaggttc
      241 tgatcctgtt gaaatggcaa aggctgcaaa gataaatgtt gagtatggtg ccgatattat
      301 tgatattaat atgggttgcc cggcaaaaaa ggtgaataaa aatatggcag gctctgctct
      361 tttacgtaac cccaaattag tcgctcaaat attagattct gtagtcaatg ccgttgatgt
      421 ccctgtgaca ctaaaaatta gaacaggttg ggatcaggaa aatagaaatt gtctgcaaat
      481 tgcgaaaatt gcagaacaag ccggcataaa agctctcact attcacgggc gtactcgaag
      541 ctgtttattt aatggcgaag ctgaatatga aagtattaag gcagtaaaag aagcaatttc
      601 tatcccagtg atagcaaacg gagacattga ctctgcggaa aaagcaaagt ttgttctcga
      661 ctataccaaa gccgatgcaa tcatgattgg gcgcgctagc tttggaaatc cttggatatt
      721 taaggaaatt aatgattttt ttgaaactgg attatcttca ccattatcaa ttgataaaaa
      781 ataccaatta atgtttaaac atattgagga tttgcaccaa ttttatggtg aagaaaaagg
      841 ttatcgtatt gcacgaaaac atgttggttg gtataccgaa aaattaaatc cggaatcaaa
      901 ttttaaacgc acttttaata cactaagttc cacaaaagag caactttatg cattagaaga
      961 ttttgttaaa tcgattcatt tagaatctca attaagaaaa gttggataaa aaaatgttag
     1021 agcaacaacc tacacaaaac ccattaacag taacaatgtt aaatgcacaa gcgcagcagg
     1081 ttaataaacc acttcgcgat aatgtgaaag cagcattaaa aaactattta tctcaattaa
     1141 atggtgaaga tccaacagaa ttatacgaat tagtattatc tgaaatcgaa cacccaatgt
     1201 tagatatggt gatgcaatat actcgcggca atcaaactcg tgcagcaaca atgctaggca
     1261 ttaaccgtgg cacattacgc aaaaaattaa aaaaatacgg tatgggttaa tctaaccgac
     1321 cgttaaaaca cgattttaat tcgtatgtaa ttagttagag ccaacaaaaa acgctgaata
     1381 tactattcag cgtttttcta ttttattttg ccgtctaaaa cttcatttca aatgctaaac
     1441 tgaaatttct ccccggagcg gcaaatcgag tgtaattgct accccggtct tggtttactg
     1501 cattcacacc ggattggcgc actgattccc aagtggaata tttacgatta gtcacattat
     1561 aaattcctcc acgtaaggtg tagtttttat aattgatgta accggtaata tcatgggtgt
     1621 accaagaacc ggtcagtttg ccacctaaat caacgcgatg tatgccgtga tgacgtgtgc
     1681 ctgccacctc atcggcgttt ttggctttag aataggtggt agtaatgcca attccccatt
     1741 tttcttctgg gtgatcgtag ccgaatccaa gcacatagcg tgctggctga atcgcatcta
     1801 gaataggatc gtttacgcta tcgagtcttg agtcggttag tttccgctct tttactttta
     1861 cacggttata agcaactgat gaataaaaac catctggcag catagataaa ataccgtgta
     1921 agtcaaattt agcgactaaa tttatcccgt ttagtgctac atcttgaata ttatggtagt
     1981 tatagaaaca gtgtgatttt tgtattattc ttttacatgc tgtcgcaatc atatttttat
     2041 agttattacg gaaatagctc acatcaaggt aaccaaattg acctttaaag ctaactccaa
     2101 tctcatgatt agtcgatttt tctggctcta actggtggct acgttgcgcg cgttgcacat
     2161 attcgttatc tttaagccca atatgatcac gtttaccata aagttcatag aatgcaggca
     2221 ctctaaaacc gtttgaaatg cgataagaaa gcgatacaaa ctctgttggt ttaagcgtaa
     2281 tcccaccatt ccaagaccaa tttcgataag ttcggctaag tgtccacgga tcatcagatt
     2341 taaatcgatg ttgatcaaaa cgcaccccta atcccaaatc cacatactcg cttatcacta
     2401 gatcgcggaa gctaacaaaa tggctatcgc ctttaatttt acgcggttca cagtttaaga
     2461 tgtcgccgcg tgtattatta cacaaagata ccgtttcaat actgcgtgga tcgcggcgat
     2521 aaatatatgg attatctaaa cgccctctac cgcggtagct ggtataaccg cctttggtat
     2581 attgggcagt catatcccca tgatccataa gcgaattaaa tcgatcaaag cccaacccta
     2641 aattcagttt gtgggtttgc ttaaatacgc cttgaccagc atttagcgct ttatcaaatt
     2701 ctaaatgaat gacacgatgc ttttcttggt aattattacg ctcagttcga tacatagacc
     2761 aagatttatc caaagtcggg cggcaatttt tatctaccac aggataatcg ctacaatgca
     2821 agcgatgtag ccggctatat agttcaatat cttgtttatc cgcactgagt ttaatgctat
     2881 ccaaccagcg attattctct ggtttatatt tataggtgaa tcctaaacga cgtttgtggt
     2941 gacgttcatc aaaaaacttc acatggctat agcgcaaccc ataagggatc cttggcttga
     3001 acactaagcc atctaaaata ttatcccctt cataaacata gttcctaagt gataagttaa
     3061 tatcgtcttt tgtatagtaa gctggcgttt gcatatcacg gatatcgtag cgttgttttg
     3121 tatcttctaa gattgcgcca agataatgag acgagttaaa gtgatagcca aacttcataa
     3181 aaacagaatt actcttgtaa tctaaagggt ttggtgcaat gcgatcttta ccggtatatt
     3241 cttgggctga gagctgctca gtgcgataag gaatctgctc aagctgtttg cgctcttcag
     3301 gcgtatattc cggaaatgtt ctcactggga aattatcgcg gttcaactta acccttgcac
     3361 gaggagtaca atctaatgtt gggcaagtat cttccacaat aaaaaaagat cctccaggag
     3421 cattctgtgc ggaatttggg taaagatcgt agcgattttc aaagccggtt atacgccgaa
     3481 tattatgttt taatttattt gcctcggaat gaattttggt ctctttaccg tgtcggtgag
     3541 ttgcaataac aagtgcttca aaaccacccg cctcaccagc cgctgcgata gactgtaaaa
     3601 aatggctatt tttgctggca taagaggtct tactatctaa gccccaatgc tgcccctctt
     3661 taataatatc ctgcgcatct ttagtacgaa aaccaatagc accacctagc gcaccagagc
     3721 catattccgc agaacttgct cctttgctta actcaattga acgaatgttt tcatactcaa
     3781 tctcattaat tgcaccacca ttagcatctg aacctagcgt atgataatgc tgcgcttgtg
     3841 gtagcccatc aacaagtaag ctgacacggt ttttatctac acctcgaatg gcatagccac
     3901 tacttgcacc gcgaccttgt tccaccaccg aaataccagg gtcatagcga gttaaatcac
     3961 gaataccaag aatttgattt ttactcattt cgtgataatt tttcactact ttccccaagc
     4021 cagttacttc gttttgacgt tcgtgagcat aatggctctc tgtcacaata acttcatcca
     4081 gaacagctag atcgttattt tcttctattt ttttattttc agtcgcagca ccgtatgaat
     4141 gagaaagagc aaataacact gttaaggcaa caggtgaata gcgaaaatga tgatatttca
     4201 ttatcattag atagggtctc ctttatttaa attttgcaaa tttttataaa aaattgcccg
     4261 cttgctatct acaaccagca aaggggaatt atgattattt ttctatttga cgttttccgc
     4321 cgaatgtaat actgactcta tctttatccg tttctgcact atgtacaata ccacctaatt
     4381 ccgtcgcctt agggccataa aagccccctt caaattgagt atttatatgc actaccgtgc
     4441 caccattcgt acttttcgga tcaagattaa aaccagaatt gcccgttttg gctgtgcctg
     4501 caaaaccatt ccctttcaga ttgccttcaa gtgtcagcat tgggcgttct tcaacaccat
     4561 cattagcaat cagcttgcca ttaatttttt tattgacaaa atctacatcg aatagtgaac
     4621 gagtaccact ttcgctgtta cttggcgatt cagcccctgc cgagccagat ttactgtaca
     4681 ttaccccgtc ccaagtacct cgatatttta ctgtccctgt tggcaaatct gccacagctg
     4741 tacgttctcc tactaaatat tggtgagcag tatcactatt atttccctcg ctataagtcc
     4801 caaatttgac ataatctaaa ttattacagc agactgaaac tcgatacgtt ttatcatcaa
     4861 tagtacgggt aaccaaatca tcaaatttca tctcggcaaa gctttttttg ccttccgcaa
     4921 taagtggaat ctgcttattg ttaataatca aatgtgtcgc attcccgaaa gtatccatct
     4981 cagatttatt taaatcttta agtttaattt tgaaagcatc aaaggcagat tcactatcat
     5041 cttttttagc atctcgtttg ccagcaaaaa caacaaatac cgacttatca tcagctaaga
     5101 atttaccggc aagttcttga gccttcccac caaaaaatcc accttcaagt gtcgtagagc
     5161 ttttaccaaa ataaggatca tcggtatttt ttgcgtttgc tttaccagaa aagcgattac
     5221 ctttaatttt tgcatcaata tcgtaaattg ttgtaatttt ttgctctttg ctacgactaa
     5281 catacccatt acgaaccaat gttccagtta aagttttatt agcaaaatca gccgtaaatt
     5341 ctcctgtatg ccccattggt ttttcagaat catcaacatt aatatcgtaa ctattttccg
     5401 gtgtggcgcc aatattgatg cctgcggaat tagaaaaata tttactatct ctcccttttc
     5461 tcgcatcggt agtaaaatcc caatatcctt tataagtagc tttttgagta ggtattgctt
     5521 ttgccggctc tatacctaaa tagtaaacat acccatgtgc acctgttctc aaaatttctt
     5581 taggctttat ttcaggtttt gcaccagaat gcagaacata gccggaacga acaaaattaa
     5641 aatctctagt atacaactta tgatttctat catgagtatg gctaaatcca tcagcactac
     5701 taccgtacgc tttaacctct tcttcatacg gaatttttgt cagatctcca gtaatctctt
     5761 ttatatcacc aataggggct acatcttcct tcccctgcgg aaagagatta cgacgcggaa
     5821 ttttggctga aaaaccaaac cccggctgca ataaagcttc agtttcagaa gcgcttactg
     5881 tacgtcttgc gttactatta tcatcttgta aacttggctt tttgggggta gtttgcgttt
     5941 gagattcaac tttggcagat tgaacatcaa agcttccacc atttgaggaa caagcagcaa
     6001 gtagcg
//
LOCUS       DANLRK010000156         5949 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.156, whole genome shotgun sequence.
ACCESSION   DANLRK010000156 DANLRK010000000
VERSION     DANLRK010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5949
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.156"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            18..1835
                     /locus_tag="PXA33_002125"
     CDS             18..1835
                     /locus_tag="PXA33_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme anaerobic degradation radical SAM
                     methyltransferase ChuW/HutW"
                     /protein_id="HDL5121426.1"
                     /translation="MIIKDTHWTSQQASPNAFPERQALMPIWGGKNIERNQWQSLWLE
                     QAPNALLQDGLAYFHIPFCASHCIFCGFYRNAWKENYSQIYTDRVISELRYESSLWRG
                     QGKIHAVYFGGGTPTALHTEDLVRLIRACYRYLPLAEDCEFTIEGRISHFDIDKAKAC
                     IEAGVNRISIGIQTFNSEIRKRLGRKHSGEEAQTYLKALSELNAVIVADLIFGLPNQT
                     DDIWQNDIEIATSLPISGLDIYAFNNYPFLPINRLIEHGKLPAPAGFDTQSLHYAYAV
                     EKLKAQGWQQVSNNHFAFPNRGERNRYNTLVKSNMPCLAFGSGAGGNFGGLSYQVQSD
                     LKTYLDSPLDQKSLSFLSKHGEHKALLGVIQHSMELGYLDTTPFEQNAKAEKLLQQWE
                     ALDLLKREKNQVYLNTSGRYWSPTLIRKLMLTLPQSSKDNNMITPLTKELENKLRTNL
                     KENPGQILEMLAGMNQCSLESVINCLPKEMVMQTGGERFVEIMQAIAGWNEAVTFIIH
                     TPDAIAEVTAKLPQGKIGRGFYNFEHGVDGGIHGHLRYENCAAIYLLDRPFMGKRTVA
                     LTFINQSGEAMFKIFVGRDEQGQLRENQIQAMRKLIGGC"
     gene            1838..2455
                     /locus_tag="PXA33_002126"
     CDS             1838..2455
                     /locus_tag="PXA33_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL5121427.1"
                     /translation="MILVCGANGLLGRAICDKLPREKTIAVLRKDAMDTFFSERNIAV
                     EIADLLDLESCNALLQKTQPEILITVVGGKNEQGIRSDKTANINLIKAVEKYSPTTKV
                     VLVTSVGCAEQWDLISPLAQQALGEALKAKTEAEAYLQQTALNWLIIRPGGLNNDEVN
                     DEYQIVRDLPNERKMYVSRKSVATGIVRLLDSTENHQIFSIINRG"
     gene            complement(2539..3150)
                     /locus_tag="PXA33_002127"
     CDS             complement(2539..3150)
                     /locus_tag="PXA33_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentahemic C cytochrome"
                     /protein_id="HDL5121428.1"
                     /translation="MKSLSKIAFCFLAFIMPTFCFAGSNSYTTETIPLFADENLTQKI
                     GQLEAGAEIKLVQTTAKAAEIELNLWRKSKGFGRIWYHSFSKNITDAVFEKSFISNNP
                     LLEIVESKEDPNTGLVWQKVKMNVWVENAEFTQDLTNFWANTKNTYKTECSVCHKQRD
                     PKMHDANEWIAVFNGMVGFTDMDEATQKKVLRYLQMNAASSSQ"
     gene            3345..4253
                     /gene="glyQ"
                     /locus_tag="PXA33_002128"
     CDS             3345..4253
                     /gene="glyQ"
                     /locus_tag="PXA33_002128"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017806840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HDL5121429.1"
                     /translation="MSTKFNVKTFQGMILALQDYWASKGCTIVQPFDLEVGAGTSHPM
                     TCLRALGPEPMAFAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLDS
                     LKMLGFDPTQNDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPV
                     TGEITYGLERLAMYIQGVDSVYDLVWSDGELGKTTYGDVFHQNEVEQSTYNFEYADVD
                     FLFQCFEQYEKEAQYLLGLEKPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILR
                     IRALTKGVAEAYYASREALGFPGCKK"
     gene            4333..4593
                     /locus_tag="PXA33_002129"
     CDS             4333..4593
                     /locus_tag="PXA33_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121430.1"
                     /translation="MKPILYFADWCPDTAPFVAELDRLGIEYEPCDITKGGSTLKPFL
                     KLRDTHSAFDHAKANGYIGIPALLIEGEKVVLDLAELEGIFG"
     gene            4636..5670
                     /locus_tag="PXA33_002130"
     CDS             4636..5670
                     /locus_tag="PXA33_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HDL5121431.1"
                     /translation="MKTVIADNSAEFVNEISQLIQSSKQRMTVAVNAELTLLYWHIGK
                     RINDYILQGERAEYGQEVVKNLAQSLTEQFGKGWSKRHLNYTMQFATTFPNLEIVHAL
                     RAQLSWTHFKQIIHIDDPTKRGFYATMAAQERWSTRTLEDRIGSLLFERTAISKKPDE
                     TIIAELSLLREKGEYNQSMVLKDPYILDFLDLNDRYMERDLEDAILREIEQFLLELGA
                     GFTFVARQKRIQIDNDDFYIDLLFYNRKLKRLIAIELKTESFKHSHKSQMELYLAWLA
                     KYEQEADENPPLGMILCTGKKQEQVELLDLKNSDIHIAEYMTVLPSKAVLEQRLHLAV
                     ERAKERFLLK"
BASE COUNT         1830 a         1119 c         1324 g         1676 t
ORIGIN      
        1 actattttgt ttcgataatg attataaaag atacacactg gacatctcaa caagcctctc
       61 caaatgcttt cccagaacga caggcactta tgcctatttg gggtgggaaa aatattgaac
      121 gcaatcaatg gcaatcttta tggctggaac aagcaccaaa tgcgttatta caggatggtt
      181 tagcttattt ccatattccg ttttgcgcta gtcattgtat tttttgtggt ttttatcgca
      241 atgcttggaa ggaaaattat agccagattt atactgatcg tgtgattagt gaacttcgat
      301 atgaatcttc actatggcga ggacaaggta agattcatgc cgtttatttt ggtggaggaa
      361 cgccaacagc gttacataca gaagatttag tacgattgat tcgagcttgt tatcgttatt
      421 tgccactcgc tgaagactgt gaatttacta ttgagggtag aattagccat tttgatatag
      481 ataaggctaa ggcttgtatt gaagcaggcg tgaaccgtat ttctattggt attcagactt
      541 ttaactccga aattcgcaaa cgtttagggc gcaaacatag tggcgaagag gctcaaactt
      601 atttaaaagc cttatcggaa ttaaatgctg tgattgttgc tgatttaatt tttggcttgc
      661 caaaccaaac agatgatatc tggcaaaacg atattgaaat tgcgactagt ttaccgatct
      721 caggcttaga tatttacgcc tttaataatt atcctttcct gcctattaat cgcttaattg
      781 aacatggaaa attacctgcg cccgcagggt ttgacaccca aagcttacac tatgcttatg
      841 cagtagagaa gttaaaagca caaggttggc agcaagtgag caataaccac tttgcttttc
      901 caaatcgagg tgaacgcaat cgctataata ctttggttaa atcgaatatg ccttgtttgg
      961 catttggctc cggtgcgggg ggaaattttg gtgggctgag ttaccaagta caatctgatt
     1021 taaagaccta cttagattcg cctcttgatc agaaaagcct ttcttttttg agtaaacatg
     1081 gtgaacataa agcactgctt ggtgtgatac aacatagcat ggaattgggt tacttagata
     1141 caacgccttt tgaacaaaat gcgaaagctg aaaaattatt gcaacaatgg gaagcgcttg
     1201 atttactgaa gcgtgaaaaa aatcaagtat atctcaatac cagtggtcgt tactggtcac
     1261 caacattaat tcgtaaacta atgttaacac ttcctcaatc aagtaaggat aacaacatga
     1321 taacgccatt aactaaagaa ttagaaaata aattgcggac aaatttaaaa gaaaacccag
     1381 ggcaaatttt agaaatgctg gcaggaatga atcaatgtag cttggaatcg gtaattaact
     1441 gtctgcccaa agaaatggta atgcaaacag gtggtgagcg ttttgtggaa attatgcagg
     1501 caattgctgg ttggaatgaa gctgtgactt ttattattca tacgcctgat gcgattgcag
     1561 aggtaactgc taaattgcca caaggtaaaa ttgggcgagg tttttataat tttgaacatg
     1621 gtgtagatgg aggaattcac ggtcatttac gctatgaaaa ttgtgctgct atttacttac
     1681 ttgatcgccc atttatggga aaaagaacgg tggcgttaac ttttatcaat caaagcggtg
     1741 aggcgatgtt taagattttt gttgggcgtg atgaacaagg tcaattacga gaaaatcaga
     1801 ttcaagccat gcggaaatta attggaggat gctagtcatg attttggttt gtggggcaaa
     1861 tgggttgtta ggacgagcaa tctgtgacaa attacctaga gagaaaacga tagccgtctt
     1921 acgaaaagat gctatggata cgtttttcag cgaacgaaat atagcagtag aaatagcaga
     1981 tttattagat ttggaatcct gcaacgcctt gttacaaaaa acacaaccgg aaatcttgat
     2041 tacggtagta ggaggaaaaa atgaacaagg tattcgtagc gacaaaacag caaatattaa
     2101 tttgataaaa gcagttgaaa aatattcacc aactacgaaa gtagttttag ttactagtgt
     2161 tgggtgtgca gaacaatggg atttaattag cccattagca cagcaagctt taggtgaagc
     2221 actaaaagcc aagacggaag ctgaagctta tttacagcaa acagcattaa attggctgat
     2281 tattcgaccc ggtgggttaa ataatgatga agtaaatgat gagtatcaga ttgtgcgtga
     2341 tttacctaat gaacggaaaa tgtatgtaag ccgtaaatct gtcgcaacag gcatagtgcg
     2401 tttactggat agtacggaaa atcatcaaat cttctctatt attaataggg gatagtatat
     2461 ttcaacgaag gcaagcgggc atattttatg gttattttgc aactataaaa cagaccgctt
     2521 gtgagatctt tttctatttt attgagaact ggatgccgca ttcatctgca agtagcgaag
     2581 cacctttttc tgggtagctt cgtccatatc ggtaaaaccg accattccgt taaatactgc
     2641 aatccattcg tttgcatcat gcattttagg atcacgttgt ttatggcaca cgctgcattc
     2701 tgttttatag gtatttttgg tgttcgccca aaagttagtg agatcttggg taaattcggc
     2761 attttccacc cacacgttca ttttcacttt ttgccatacc aagccggtgt tcggatcttc
     2821 cttgctctct acaatctcta acagtgggtt gttgctgata aaagatttct caaataccgc
     2881 atcggtaata tttttactga aagaatgata ccaaatccgc ccgaaacctt tgctttttcg
     2941 ccataaattc agctcaattt cagcggcttt tgcggttgtt tgcaccaatt taatttctgc
     3001 tccggcttct agctgcccga ttttctgtgt caaattttca tcggcaaata aagggattgt
     3061 ttccgtggtg taactgtttg aaccggcaaa acaaaatgtt ggcatgatga atgccaagaa
     3121 acagaaagcg attttcgata atgatttcat cataaacccc acaaattaga tgagaaatta
     3181 ggttatatgt tacacaaatt gagcaaaaaa gcgttgattt acatcataaa actgatttta
     3241 tgtcgatttc ttaatttttt gcaaaaaaat cccaaaaaat gactgcttac aagctataat
     3301 aaccagtatt tttcgaccat ttttcaaatt caagagaaaa taaaatgagt acaaaattca
     3361 acgtaaaaac ctttcaagga atgattttag ccctgcaaga ctactgggca agcaaaggtt
     3421 gcaccattgt gcagccgttc gatttagaag tgggggcagg cacctcgcac ccaatgacct
     3481 gcctgcgtgc gcttggccct gagccaatgg cgtttgccta tgtgcaacca tctcgccgcc
     3541 cgacagacgg tcgctacggc gaaaatccga accgtttaca gcactactac cagttccaag
     3601 tggtgattaa accttcgccg gataacattc aagaacttta cctcgacagc ctgaaaatgc
     3661 tcggtttcga cccaacccaa aacgacattc gttttgtgga agacaactgg gaaaacccaa
     3721 cgcttggggc gtggggctta ggttgggaag tgtggctgaa cggaatggaa gtgacccaat
     3781 tcacctactt ccagcaagtc ggcggcttgg aatgtaaacc ggtgacaggc gaaatcacct
     3841 acggcttaga gcgtttggcg atgtacattc aaggcgtgga cagcgtgtac gacctcgtgt
     3901 ggtctgacgg cgaactcggt aaaaccacct acggcgacgt attccaccaa aacgaagtgg
     3961 agcaatccac ctacaacttt gaatacgctg atgtggactt cctgttccaa tgcttcgagc
     4021 agtacgaaaa agaagcccaa tacctgttag gcctagaaaa accattgcca ttgccggcat
     4081 acgaacgcat tttaaaagcc gctcacagct ttaacttatt agatgctcgt aaagcgatct
     4141 cggtaactga acgccaacgc tacattctac gcattcgtgc cttaaccaaa ggcgttgctg
     4201 aagcgtacta tgccagccgt gaagcgttgg ggttcccggg gtgtaaaaaa taatcgtagg
     4261 gacgccacgc ttggcgtccg tcaataaatt acaaaacgga cacgaagcat cgtgtcccta
     4321 caaggattta cgatgaaacc gattttatac ttcgccgatt ggtgtccgga taccgcccca
     4381 tttgtggcag aactagaccg cttgggcatt gaatatgagc cgtgcgacat caccaaagga
     4441 ggctcaacac ttaaaccatt tttgaaactg cgagacacgc attctgcctt tgaccacgcc
     4501 aaagccaacg gctatattgg cattccggca ttgctgatcg aaggcgaaaa agtcgtgctg
     4561 gatttggctg aattagaggg tattttcggc taacaagcgg ttgaatttgg cggaaatgtt
     4621 gcaaaggagc caataatgaa gacagtaata gcagataatt cggctgaatt tgtgaatgaa
     4681 atttcacaac ttattcaatc gagtaagcaa cgaatgaccg ttgcggttaa tgcagaatta
     4741 accttgcttt attggcatat cggaaaacgg atcaatgact atattttgca gggtgaacgt
     4801 gcggaatatg ggcaagaagt ggtgaaaaat cttgctcaaa gtctgacgga gcaatttggt
     4861 aaaggttgga gtaaacgcca tttaaattat acaatgcaat ttgctacaac tttccctaat
     4921 ttggaaattg tgcacgcact gcgtgcacaa ttaagctgga cacattttaa gcagattatt
     4981 catattgatg atccaacaaa gcgagggttc tatgcaacaa tggctgcaca ggagcgttgg
     5041 agtacaagga cattagaaga tcgcattggc tcattattgt ttgaacgcac ggcaatttcc
     5101 aaaaagcccg atgaaaccat tattgctgaa ttgagtttgc tacgtgaaaa aggcgaatat
     5161 aatcagtcaa tggtgttgaa agatccctat attttggatt ttctcgacct taacgaccgt
     5221 tatatggaac gtgatctcga agatgcgatt ctacgagaga tcgaacagtt tttactggaa
     5281 cttggggcgg gttttacctt tgtcgcccgc caaaaaagga ttcagattga taatgatgat
     5341 ttctatatcg atctgctgtt ttataaccgt aaactcaaac gcctgattgc gattgaacta
     5401 aaaaccgaaa gcttcaaaca ttcccacaaa agtcaaatgg aactctattt ggcgtggctg
     5461 gcgaaatacg agcaagaagc agacgaaaac ccaccgcttg gaatgattct ctgcaccggt
     5521 aaaaagcagg agcaagtgga attgttggat ttgaaaaatt ccgatattca tattgcggaa
     5581 tatatgaccg tgttgccgag taaagcggta ttggaacaac ggttgcattt ggcggtggaa
     5641 agggcgaaag agcggttttt actaaaatag caagcggtta aaatcagcgg aaatgttgca
     5701 aaatgaaagt atgatggaaa cgatttggtt gcatattccg ttggtgggtg gttagagatt
     5761 ttcaaatctc ccccctcccc ctctttggct aaagaggggg cgatcattta gtgagtaatt
     5821 ttaattcatc tgactattta caatcttaat gaagaaagat gaattataaa tttacgaaga
     5881 tcaaattccc ctttttagca aagaggggct aggggagatt tggcagaagt gaaaacgtag
     5941 tgagagatt
//
LOCUS       DANLRK010000157         5820 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.157, whole genome shotgun sequence.
ACCESSION   DANLRK010000157 DANLRK010000000
VERSION     DANLRK010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5820
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.157"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(<1..399)
                     /locus_tag="PXA33_002131"
     CDS             complement(<1..399)
                     /locus_tag="PXA33_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014668079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMP-N-acetylneuraminate-beta-galactosamide-
                     alpha-2, 3-sialyltransferase"
                     /protein_id="HDL5121432.1"
                     /translation="MNLIICCTPLQVLIAEKIIEMHPNERFYGVMLSTVKNAKFDFYQ
                     ARLAQKCEQFFAMQQHSERIQLLKEIIYLKRTFGGKKFNKVFLANINELEIQFLLSAI
                     SFQELNTFDDGTANIVQNSIFYQAENIGLNR"
     gene            complement(396..1601)
                     /locus_tag="PXA33_002132"
     CDS             complement(396..1601)
                     /locus_tag="PXA33_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005694190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HDL5121433.1"
                     /translation="MKAVKDSVIYLIGELASKSIPFLLLPYFSRKLGVAGYGELSYYQ
                     TFAALFVIFIGLSQDGAVARYFYFYGKRSLNLVLSTGYLYTLCATLLILLGCWYFQSE
                     IIAYVAIGAMFQSFLNVQLSVRQCQKQALAYTTIQLMSAIFSAVITVAMMEYFKTDLV
                     EKRILAILMSNGLVFLIAYFLYAKRAKTKKFSLARHKTALLYLFGFGIPLILHHSSIF
                     LKGQLDRVFIYHKFSEEDLGLYAMGAQIAMILAIVVQALNKATLPYFYEALKQKKINV
                     SHIHKWALYALFIVPLPAIVMWFIPEELVVWILGSQFVGTKYYIILFLIATMLVVPYL
                     ILVNYLFFYGKNKLISLCSILSTIFYLVALFALTFSKIEYIPFASIIGAISIIPILYI
                     MTAKVGKTV"
     gene            complement(1611..2795)
                     /gene="neuC"
                     /locus_tag="PXA33_002133"
     CDS             complement(1611..2795)
                     /gene="neuC"
                     /locus_tag="PXA33_002133"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HDL5121434.1"
                     /translation="MKNIAYITGSRAEYGIVKRLLQKLAQDPTVNFSLVVTAMHLDPQ
                     YGNTVTVIEQDGFEIAARIPLTLNSENNQTIIHSMAECLDRFGEHFQQHRYDAVIVLG
                     DRYEMLAVATAAAMHNIPLIHLHGGEQTLGNYDEFIRHCITKMSKLHLTSTEEYRQRV
                     IQMGELPENVVNTGSLGAENSLDLVLPTQAELVERLGIPASPYFLVVFHPETLTDVSV
                     LEQIEALLNAFEQFKDKYQFVFIGSNSDTNSEQIYARFKAYTDENQFKFFTSVKPEEY
                     LALIKYSNGLIGNSSSGLIEAPSCGVGTLNIGNRQQGRVRDESVIDVESNEQAIIDGI
                     EKLISADFQHRLPSMKNPYFQDNAMEKAYNAIKIFLADSHHTQPKGFYDVAFSQAVKK
                     SE"
     gene            complement(2796..4055)
                     /locus_tag="PXA33_002134"
     CDS             complement(2796..4055)
                     /locus_tag="PXA33_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase"
                     /protein_id="HDL5121435.1"
                     /translation="MKKIAIITARAGSKGLPNKNVLLANGKPLMAYSIEAAIESGQFE
                     KIIVSTDSQEYIDLFSHYPIEFIKRAEHLASDKASSFVVIEDVLNQYRSVEFDYFMLL
                     QPTSPLRSGEHIKESCEKFEKNFEQFDFLVSVSDAHKPTTLTRMIEEDECLKHFQLDY
                     SNYARQLHHPEYSPNGAIFTAKPEAYLKQKHFYGAKCLAYFMDKDVSIDIDDRQDFEY
                     FYFILQQRNKEKILLETIKRSILMKKENFKEVKEIALIGHSIIEYWDIEHLNGKAVNN
                     LGIAGISTKQYSDLILDEGLISALPKDIILMFGTNDIVYAGWSDEKILADINHLIAQL
                     TAIRGDINLYFLEITPTAFRVDRNNNDIRLLNQYLKDNLAVKWISLDQAFSDKYGKLD
                     LSYSDDGLHLNAKGYEKLTAILEKELN"
     gene            complement(4115..5152)
                     /gene="neuB"
                     /locus_tag="PXA33_002135"
     CDS             complement(4115..5152)
                     /gene="neuB"
                     /locus_tag="PXA33_002135"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HDL5121436.1"
                     /translation="MVNVFIVAEIGCNHNGDPALAKKMVDVAKECGVDAVKFQTFKAD
                     KLISKYAPKAEYQKVTTGTADSQLEMTRKLELPYEEFVKLEAYARSLGLEVFSTPFDF
                     ESIDFLASQQQKVWKIPSGELTNLPYLEKIARLPIEGKTIVISTGMATIEETKASLKV
                     LEDNGVAKKDITILHCNTEYPTPYEDVNLNAFHQLKQEFGDYSLGFSDHSAGYFAGLA
                     AVPYGITFIEKHFTLDKNFEGPDHKASVTPEELKLLCEGIRAVEKSLGSSEKLVTNSE
                     AKNKIVARKSIIAARPIKKGEIFTTENITTKRPGNGISPMFWYDVLGKEAQQDFEEDQ
                     LIQDSRFENQM"
     gene            complement(5152..5796)
                     /locus_tag="PXA33_002136"
     CDS             complement(5152..5796)
                     /locus_tag="PXA33_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDL5121437.1"
                     /translation="MQQKSKREKLILIGAGGYAKSVLDSLDHEQYEFCGLIDNFKPEG
                     SVHLGYPILASTIDCFSKRDNYHYFICIGNNSYRLEKFSKLQKYKCRIINVVDKTALV
                     SKNSTLGIGVFVGKMAIVNSGVTVGDNVIINTKSLVEHGCFIGSHCNISTNTTLNGDV
                     IVEDHAFIGSSSVVNGQLRVGESALVGSGAVVIRNVEPRTVVAGVPAKYIKDIK"
BASE COUNT         1846 a         1199 c          954 g         1821 t
ORIGIN      
        1 tcgatttaac ccaatatttt cagcttgata gaaaatactg ttttgtacaa tattagctgt
       61 gccatcatca aaggtattga gttcttgaaa tgaaatggca cttaacagaa attgaatttc
      121 taactcattg atatttgcta aaaagacttt attaaacttc ttaccgccaa aagtacgttt
      181 taaataaata atttctttta ataattgaat acgctcgcta tgttgttgca tcgcaaaaaa
      241 ttgttcgcac ttttgggcta atctagcttg gtaaaaatca aatttcgcgt tttttaccgt
      301 agaaagcatc accccataga atcgctcatt agggtgcatt tcaatgattt tttcagcaat
      361 caacacttgc aagggcgtgc aacaaataat taaattcata cggtttttcc tacttttgcc
      421 gtcattatat aaagaatcgg aataatgctg attgcaccaa tgatgctggc aaaaggaata
      481 tattcaattt tagaaaaagt taaagcaaat agtgcaacta aataaaaaat agtagataaa
      541 atcgagcaaa gcgaaattag cttatttttg ccataaaaaa acaaataatt gactaaaatg
      601 aggtaaggca caaccaacat tgtcgcaatt aaaaagagaa taatgtagta ttttgttcct
      661 acaaattggc tacccagaat ccataccact aattcttccg gaataaacca catcacaata
      721 gcaggtaatg gtacaataaa taaggcatac aacgcccatt tgtggatatg gctgacatta
      781 atttttttct gctttaaggc ttcataaaaa taaggcagtg ttgctttatt taaggcttga
      841 accacaatgg ctagaatcat cgcaatttga gcccccattg catataaacc taaatcctct
      901 tcgctaaatt tatggtaaat aaatactctg tctaactgtc cttttaagaa aatactggaa
      961 tggtgcaaaa tcaacggaat accaaagccg aataaataca ataaagcagt tttatggcgg
     1021 gcaaggctaa atttcttcgt ttttgctctt ttagcataca aaaagtaagc gattagaaag
     1081 actaagccat tactcatcaa aatcgctaaa atccgctttt ccactaaatc ggtcttaaaa
     1141 tactccatca ttgcaacggt aataactgcc gaaaaaatcg ccgacattaa ttgaatcgtt
     1201 gtataagcaa gtgcctgttt ttgacactgc cttacactaa gctgtacatt caaaaacgac
     1261 tggaacatcg ccccaatcgc tacataagca ataatttcag actggaaata ccagcaaccc
     1321 aacaggataa gcaaagttgc acaaagagta tataaatacc ctgtactcaa gactaaattt
     1381 agtgaacgct taccataaaa atagaaatag cgagcaaccg caccgtcttg gctaagcccg
     1441 ataaaaatta caaataaagc agcaaaggtt tggtagtagg aaagctcccc ataacctgcc
     1501 acgccgagtt tgcgtgaaaa gtagggtaag agcaagaaag gaattgactt tgacgccaat
     1561 tccccaatta aataaatcac actgtcttta actgctttca tagttgcaaa ttattctgat
     1621 tttttgaccg cttgcgagaa agcaacatca taaaatcctt tcggttgagt atggtggctg
     1681 tctgctaaga atattttgat tgcattgtaa gccttttcca tcgcgttgtc ttgaaaatat
     1741 ggatttttca tactcggtaa acggtgttgg aaatcggctg aaatcaattt ttcgatacca
     1801 tctatgatag cctgttcatt actctctaca tcaattacgc tctcatcccg tactcgccct
     1861 tgctggcgat taccgatatt tagcgtacca acaccacaac tcggtgcttc aatcaagcct
     1921 gatgagctat taccaatcaa cccatttgaa tatttaatca acgctaaata ctcttccggt
     1981 ttgactgagg tgaaaaactt aaattgattt tcatcggtgt aagccttaaa acgtgcataa
     2041 atttgctctg aattggtgtc tgaatttgag ccgataaaga cgaattggta tttgtcttta
     2101 aactgctcaa aagcattgag taacgcctca atttgctcca acacggaaac atcggttaag
     2161 gtttccgggt ggaagaccac taaaaaataa ggcgatgccg gaatccctaa tctctcgact
     2221 aattctgctt gggtcggcaa cactaaatcc aaactattct ccgcccctag agaaccagta
     2281 ttcaccacat tttcgggcag ctcgcccatt tgaatcacac gctgtctata ttcttcagtt
     2341 gaagttaaat gcaatttcga cattttggta atacaatggc gaataaattc atcgtaatta
     2401 cctaatgttt gctcaccacc gtgcagatga atcagcggaa tattatgcat cgccgcagcc
     2461 gttgccaccg ccaacatctc gtaacgatcg cctagcacaa tcaccgcatc ataacgatgt
     2521 tgctggaaat gttcgccaaa tcgatctaaa cactctgcca ttgaatgaat aatggtctga
     2581 ttattttcac tgtttaaggt aagcggaata cgagctgcaa tctcaaagcc atcttgctca
     2641 atgaccgtca ccgtattgcc atattgcgga tccaaatgca tcgcagtaac aaccagagag
     2701 aaattcacag tcggatcttg tgctaatttt tgtaataaac gctttacaat cccgtattcg
     2761 gcacgagatc cggtaatata ggcaatattt ttcatttagt tgagctcttt ttccaaaata
     2821 gcggttaatt tttcataacc ttttgcattt aaatgtaatc cgtcatcgct atagcttaaa
     2881 tccaatttgc catatttatc gctaaatgcc tgatctaagc taatccattt caccgctaaa
     2941 ttatctttaa gatattgatt taacaagcga atatcgttgt tattacgatc gactctaaat
     3001 gcagttgggg taatttccaa gaaatagaga ttaatatctc cacgaattgc tgttaattgt
     3061 gcaattaaat gattaatatc tgctagaatt ttttcatcac tccagccagc atatacaata
     3121 tcattagtac cgaacattaa aataatgtct ttaggtaaag cagaaattaa accttcatcc
     3181 aaaatcagat cactgtattg ttttgttgaa atacctgcaa ttcctaaatt attaactgcc
     3241 ttaccgttta aatgttcaat atcccaatat tcaatgatcg aatgcccgat taaggctatc
     3301 tctttcactt ctttaaagtt ctcttttttc attaaaatcg aacgtttaat ggtttcaagt
     3361 aagatttttt ctttattgcg ttgttgcaga ataaagtaga agtactcgaa atcttggcga
     3421 tcatcaatat caatagaaac gtctttatcc ataaaatagg ctagacattt cgcaccatag
     3481 aagtgttttt gtttaagata ggcttctggc ttagctgtaa aaatagctcc attcggtgaa
     3541 tattcaggat gatgcaattg gcgtgcatag ttagaataat ccaactgaaa atgctttagg
     3601 cactcgtctt cctcaatcat acgagtcaat gtcgtaggtt tatgggcatc agaaacagat
     3661 actaagaaat caaattgttc gaaatttttc tcaaattttt cacacgattc cttaatatgc
     3721 tctcctgatc tcaacggcga agttggttga agtagcataa aatagtcaaa ctctactgat
     3781 ctatattgat ttaacacatc ttcaatcacg acaaaagaac tcgccttatc actggctaaa
     3841 tgctcggcac gtttaataaa ttcaattgga taatgtgaga acaaatcaat gtattcttgg
     3901 gaatcagtac taacgataat tttctcaaat tgccccgact caatcgcagc ttcaatagaa
     3961 taggccatta acggcttacc gttggctaat aatacatttt tattcggcaa gccttttgaa
     4021 ccggctcggg ctgtaataat tgcaattttt ttcataaatt tgaccgcttg ttaaacacaa
     4081 aatgatggag ctttcatccc atcactatat tatattacat ctgattttca aaacgagaat
     4141 cttgaattaa ttgatcttct tcaaaatcct gttgtgcttc tttacctagc acatcatacc
     4201 aaaacatcgg gctaatgcca ttaccggggc gtttagtcgt aatattttcg gttgtaaaaa
     4261 tttcgccttt tttaattgga cgtgccgcaa taattgattt tcttgccaca attttatttt
     4321 ttgcttccga attagtgact aatttctcgg aagaacccag cgatttttcc accgcacgaa
     4381 tgccttcaca cagtaatttc agctcttcag gtgtaaccga agctttatga tccggacctt
     4441 caaagttttt atctaaggta aaatgttttt caatgaaggt aatgccgtaa ggtactgccg
     4501 ccaaccctgc aaaataaccg gcagagtggt cagaaaaacc taaactatag tcgccaaact
     4561 cctgttttag ctgatggaac gcatttaaat tcacatcttc ataaggtgtc gggtactcgg
     4621 tattacaatg taaaatcgta atatcttttt tcgctacacc gttatcttcc aatactttta
     4681 atgaggcttt tgtttcttca atggttgcca taccggtaga aataacgatc gtcttgcctt
     4741 caatcggaag tcgtgcaatt ttttctaaat acggcaaatt ggttaattca ccagacggaa
     4801 ttttccacac tttttgttgt tggcttgcca agaaatcaat cgactcgaaa tcgaatggcg
     4861 tagagaatac ctctaacccc aatgaacgag cgtaagcctc taatttcaca aactcctcat
     4921 aaggtaactc taatttgcgg gtcatttcta attgggaatc agcggtgccg gtcgttactt
     4981 tttgatactc ggctttaggg gcatatttag agatcaactt atccgcttta aaggtttgga
     5041 atttcaccgc atcgacacca cactctttcg ctacatcaac cattttcttt gctaacgcag
     5101 gatcgccgtt atggttacaa ccgatttctg ccacaataaa tacatttacc attattttat
     5161 atccttaata tattttgctg gaacgcctgc tacaactgtg cgaggctcca catttctaat
     5221 taccactgca ccggagccaa ccaatgcact ttcacccact cttagctgtc cgttcacaac
     5281 agaggaacta ccaataaaag catgatcttc gacaattaca tcgccattta gtgtcgtatt
     5341 ggttgaaata ttgcaatgac tgccgataaa acaaccatgc tcaaccagcg atttagtgtt
     5401 aatgatcaca ttatcgccta cagttacgcc actgtttaca attgccattt ttccaacaaa
     5461 cactccaatg cctagagtgg aatttttgga caccaatgcg gttttatcta ccacattaat
     5521 tattctacac ttatattttt gtagcttaga gaatttttct aaacggtaac tattatttcc
     5581 aatacaaata aaatagtgat aattatctcg tttagaaaaa caatcaattg ttgaagctag
     5641 aataggataa cctaagtgca cactaccttc aggcttaaag ttatcgataa gtccacaaaa
     5701 ttcatattgt tcgtgatcaa gtgaatctaa cactgatttg gcatagccac cggcaccgat
     5761 caaaatcaat ttttctcttt tagacttttg ttgcattgct atcacccatg atgaatttca
//
LOCUS       DANLRK010000158         5792 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.158, whole genome shotgun sequence.
ACCESSION   DANLRK010000158 DANLRK010000000
VERSION     DANLRK010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5792
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.158"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            25..498
                     /gene="folK"
                     /locus_tag="PXA33_002137"
     CDS             25..498
                     /gene="folK"
                     /locus_tag="PXA33_002137"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HDL5121438.1"
                     /translation="MQTVYIALGSNLENPLEQLKQAVEKLKKFANEFEISPFYGSKPV
                     GPQDQPDYVNAVAKFSTDLSPTALLDKLQSIENEQGRVRVRRWGERTLDLDIILYGNQ
                     QIQTERLNVPHIEMKNREFVIVPMFDLTPDLVLPTGEKLADIYAQFKDHQMIIFS"
     gene            complement(495..689)
                     /locus_tag="PXA33_002138"
     CDS             complement(495..689)
                     /locus_tag="PXA33_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HDL5121439.1"
                     /translation="MKPATFYFAIFLLNLTACTPKVQLETPSEGITINMNVVVDHRID
                     VKMDEKSRAVITKSENKTKN"
     gene            complement(707..2482)
                     /locus_tag="PXA33_002139"
     CDS             complement(707..2482)
                     /locus_tag="PXA33_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH domain-containing protein"
                     /protein_id="HDL5121440.1"
                     /translation="MPLKTAIRISLLSQNSWGKGNIVISNSDGEITEKGLNLPLRLTG
                     NIKHGNFILYSSVPLDFQGNFDDLSVKFLPSSLLRLTGKERYLTIEDLRFPLAGIKID
                     KHGIHGRLHAILRGQSPDFNNIEFHLDGQAQNFKAGALDFFQDPKDANAVKDSWKWRI
                     WGASILKAFNSKVNLSGRGQWHKQLVRLEELKGDLATVKLADTTISKITLNNTQAIRF
                     NVKKFTLDGAVMLQSPEIAFSYGGVLPKPRVNLAFDGEVEKLRFKGAVNTTELGPLKL
                     FARRQLSQDASQIIGKLYWPEQSAQGFQPLFPFRSQWVINNGTIRGETAFSAGSKNGL
                     IAGGHLAIRNGGLSLPNGEAKGIEFSLPYRYQNGRFHFGAKKPLDVKIAQIKLGDLAL
                     DNASMKLNGYYPYTKAKPLNLNQLSFDLFGGKLNVKRFALPQTELAYLNLERIDFEQI
                     LQFMQYQQIDLKGKANAILPFWLSGKPCYICDGLLTQAETSYLTFTPELLSEMQKSGY
                     TEQILLHLVDKSTINDFRSLINVGPKGDLVLDGKLKLSSNQNKSKVNLNYNHRENLFD
                     LWHLINYGSQFEQKLENYLYQKLER"
     gene            complement(2668..3414)
                     /locus_tag="PXA33_002140"
     CDS             complement(2668..3414)
                     /locus_tag="PXA33_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121441.1"
                     /translation="MKRALKFFGIATVLSVVAIYYFLMPSHYLATPINWILPKAWKVQ
                     LPQGLNLNWQGSSLAQFQLSYRDCPLLSADKTDLGWIEKNHLSVDKLDIDYRCLALFP
                     QNEHSNSDTSLNALLAVIPDGEATIHALNWKNLPTDFNPRLQRLFEQTAIKLSLSKGE
                     IQATAAQQAVKFAANFANGRLNADLSYQPNEKEQHNLRISAEIEDNFTQLPPTISAEY
                     DWQLSYEIIANKALQKGALFSVGKKMPHKS"
     gene            complement(3418..4233)
                     /locus_tag="PXA33_002141"
     CDS             complement(3418..4233)
                     /locus_tag="PXA33_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121442.1"
                     /translation="MAHDHDSPSKLEISTHAALPMVLMLFIEMALNFYSAPKNSIFIS
                     PYLLAFFVTSLISFLVLWKGEICRGQASRLSFILPFLLIFAVGNFLYSIGFTPKHNPM
                     AVASIAAVFACLIYFRLPEDQTLYNTLMYCGLAISSIGIIQYLAIYWFELPSLFNGIR
                     ANNFAQVLLGILLAGWYLMLAKSRLDVFLKLLVKTALVVLILNYCWTAFVLYQHLQIM
                     PDMPLLSFIIYFAAQFVILLMLAWLLLGKNIKNPTAWTVATLLAILYPFANVV"
     gene            4323..4730
                     /gene="gloA"
                     /locus_tag="PXA33_002142"
     CDS             4323..4730
                     /gene="gloA"
                     /locus_tag="PXA33_002142"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HDL5121443.1"
                     /translation="MRILHTMLRVGDLERSIKFYTDVLGMRLLRRSENEQYKYSLAFL
                     GYADESESAVIELTYNWGVDKYELGTAYGHIALGVDDIYKTIEDVRAAGGKITREPGP
                     VLGGTTVIAFAEDPDGYKIEFIENKDAKAALGN"
     gene            4815..5462
                     /gene="rnt"
                     /locus_tag="PXA33_002143"
     CDS             4815..5462
                     /gene="rnt"
                     /locus_tag="PXA33_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010130043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HDL5121444.1"
                     /translation="MEQTTEQIDYKLLKHRFRGYLPVVIDVETAGLNAQTDALLELAA
                     ITLKMDEQGYLVPDQQCHFHIAPFEGANINPESLKFNGIDIDNPLRGAVAENIAIPEM
                     FKMVRKAMKEQGCQRAVIVAHNAAFDQGFLQAAIKRINAKRDPFHPFAMFDTATLAGF
                     MYGQTVLVKACQVAKIPFDGKQAHSALYDTERTAELFCAMVNRLKDLGGFPPLSD"
BASE COUNT         1714 a         1103 c         1256 g         1719 t
ORIGIN      
        1 ttgttatatt tgtaagtata ttttatgcaa accgtctata tcgccttagg atcaaacctt
       61 gagaatccgc ttgagcagct taaacaagcg gtcgaaaaat taaaaaaatt tgcaaatgag
      121 tttgaaatca gcccgttcta cggctcaaag cctgtcggac cgcaagatca gcctgattat
      181 gtcaatgcgg tagcaaaatt tagtaccgac ctatcgccca ctgcattgct ggataaattg
      241 caaagcattg aaaatgagca gggaagagtg cgtgtacgcc gttgggggga gagaacttta
      301 gatctggata ttattttgta cggcaatcag caaattcaaa ccgagcgttt gaacgtgccg
      361 catatcgaga tgaaaaatcg ggaatttgtg attgtgccga tgtttgattt aacgcctgat
      421 ttagtactac cgacagggga aaaattagcc gatatttatg cacaatttaa agatcatcaa
      481 atgattatct tcagctaatt tttcgttttg ttctcacttt tggtgatcac cgctcgggat
      541 ttctcgtcca tcttcacatc aatgcggtga tcaacgacca cattcatatt aatcgtaatg
      601 ccttcacttg gcgtttcaag ctgtactttc ggcgtacaag cggtcaaatt cagtaaaaat
      661 attgcaaaat aaaatgtggc cggtttcata tttattccat tctttatcat cgttccagtt
      721 tttgatagag gtaattttct aatttttgtt caaattgtga gccatagtta attaagtgcc
      781 acaaatcaaa cagattttct cggtgattat agtttaaatt cacttttgat ttattttgat
      841 tggaagataa ttttaatttt ccgtctaata ctaaatcgcc tttcggcccg acattaatca
      901 gactgcgaaa atcgttaatc gtgcttttat ctactaaatg taacaaaatt tgctcggtgt
      961 aaccactttt ctgcatttct gacaacaatt caggggtaaa ggttaaatag gaagtttccg
     1021 cctgtgtcag taagccatcg caaatatagc aaggttttcc gcttaaccaa aacggcaaga
     1081 tagcgttggc tttgcctttg agatcaattt gctgatattg cataaattgc aaaatttgct
     1141 caaagtcaat ccgctctaaa ttcaggtaag ccagttcggt ttgtggtaag gcaaagcgtt
     1201 ttacattcag tttaccgcca aacaaatcaa aggataattg atttaaattc aatggcttag
     1261 ctttggtata agggtagtaa ccgttgagtt tcattgaagc gttatctaat gctaaatccc
     1321 ccagtttgat ttgggcaatt tttacatcaa gcggtttttt ggctccgaaa tgaaagcgtc
     1381 cgttttggta gcggtaggga agcgaaaatt caatcccttt tgcttcgcca ttaggtaaag
     1441 ataaaccgcc gttacgaatg gctaaatgcc caccggcaat cagcccgttt ttactgccgg
     1501 cactaaacgc cgtttcgccc cgaattgtac cgttgttgat cacccattgg ctacgaaacg
     1561 gaaaaagcgg ctggaagcct tgagcagatt gctccggcca ataaagtttg ccgataattt
     1621 ggctggcatc ttggcttaat tggcggcggg cgaacagttt gagtgggccg agttcggtgg
     1681 tattcaccgc tcctttaaaa cgtagttttt cgacttcacc atcaaaggcg agattcactc
     1741 gtggtttggg tagcacaccg ccataactga aggcaatctc aggtgattgc aacattaccg
     1801 ctccgtcaag ggtgaatttt ttgacattaa atcgaatagc ttgcgtattg ttaagcgtga
     1861 ttttagaaat ggttgtatct gctaacttta cggtggctaa atcgcctttg agttcttcca
     1921 accgcacaag ctgtttatgc cattgccctc tgccgcttaa attcacttta ctgttgaagg
     1981 ctttgaggat agatgctccc caaattcgcc atttccaact gtcttttact gcgttagcat
     2041 ctttaggatc ttggaaaaaa tctaaggctc cagccttaaa attttgtgct tgtccgtcta
     2101 aatggaactc gatattgtta aaatcagggc tttgccctct taaaattgcg tgcagtcgtc
     2161 cgtgaatacc gtgcttgtca attttaattc cggcaagtgg aaaacgtaaa tcttcaatgg
     2221 ttaaataccg ctcttttccg gtcaaacgaa gtagggagct aggcaaaaat tttacgctta
     2281 aatcgtcaaa attaccttga aaatccagtg gcacgctact gtaaagaata aaattgccgt
     2341 gtttgatatt acccgtcaaa cgaagcggta aattcagccc tttttcggtt atttctccgt
     2401 ctgaattgga gattacaata ttgcctttac cccaactgtt ttgggaaagc aggctgatac
     2461 gaatcgccgt tttgagtggc aaaaagccgt tttggttgaa tgatttcggt gtgagcttgg
     2521 tgctaacaaa cccttgcagt ggaaattgtt gggtaaagtg ccaatcaaac cgcccttgtt
     2581 gaatttctag gctttcccca tcaaacagaa aagggaaatc cagtttgtta ttttcacttt
     2641 cggcaagttc aacctgccag ttgccttcta acttttgtgc ggcatttttt tgccaactga
     2701 gaatagagcg cccttttgca aggctttatt ggcgatgatt tcataagata attgccaatc
     2761 atattcagcg gaaatagtgg gcggcagttg ggtaaaattg tcctcaattt ctgctgaaat
     2821 cctaagatta tgctgttctt tttcattcgg ttgatatgat aaatcggcat tcaaccgccc
     2881 atttgcaaaa tttgctgcaa atttgaccgc ttgttgagcg gcagttgcct gaatttcacc
     2941 ttttgaaagt gagagtttaa tagctgtttg ctcaaaaagc cgctgaagtc ttgggttgaa
     3001 atcggtaggc aaatttttcc agttaagggc gtggatagtc gcttcgccat caggaatgac
     3061 cgccagcaat gcgttgagtg aagtgtcgga attgctgtgt tcattttgtg gaaacaaggc
     3121 aaggcagcgg taatcaatat ccaatttatc gacagaaaga tggtttttct caatccaacc
     3181 caaatcggtt ttatcggcac ttagtagcgg gcaatctcga taagagagtt gaaattgagc
     3241 aagtgatgag ccttgccaat tcaggtttaa gccttgtggg agttgaactt tccacgcttt
     3301 cggcaaaatc cagttaatcg gcgtggcaag atagtggctg ggcattaaaa aatagtagat
     3361 tgccacaaca gatagaacgg ttgcaatgcc gaaaaatttt aatgcccgtt tcataaatca
     3421 gactacatta gcgaaagggt ataaaatcgc taacagcgta gcaactgtcc aagcggtcgg
     3481 atttttgatg ttttttccaa gtaataacca cgccaacatc aataaaatca caaattgtgc
     3541 tgcaaaatag atgataaatg aaagcaatgg catatccggc ataatttgta gatgttgata
     3601 aagcacaaaa gctgtccagc aataattgag aatcagcacc acaagggcgg ttttcaccaa
     3661 cagtttgagg aaaacatcta aacggctttt cgctaacatt aaataccagc cggctaataa
     3721 aatgcctaac agcacttggg cgaaattgtt ggctcgaatc ccgttaaaca atgaaggcag
     3781 ttcaaaccaa taaatggcta aatattggat aataccaata cttgatatag ccaaaccaca
     3841 atacatcaat gtgttatata aggtttgatc ttccggcagg cggaagtaga ttaagcaggc
     3901 aaaaactgca gcaatgcttg ccaccgccat tgggttatgc tttggcgtaa agcctatgga
     3961 ataaaggaaa ttccctacgg cgaaaataag taaaaagggt aaaataaagc ttaaacggct
     4021 ggcttgccct cggcagattt cgcctttcca tagcactaaa aaactgatta atgaggtcac
     4081 gaaaaacgcc aataagtaag gagaaataaa aattgaattt ttcggtgcag agtagaagtt
     4141 aagagccatt tcaataaaca gcattaatac cattggcagt gcagcgtggg ttgaaatttc
     4201 aagttttgac ggcgaatcgt gatcgtgagc catagataat cccaaaaggt ataaagaaaa
     4261 tgattttaag ccaagattat gccataatag gggtcgtttt ttaacttcag ggggaagaaa
     4321 atatgcgaat tttacataca atgttacgag ttggagattt agagcgctca attaagtttt
     4381 ataccgatgt gttaggtatg cgtctgttac gtagaagcga gaacgagcag tataagtatt
     4441 cgttagcatt tttaggctat gccgatgaaa gcgaaagtgc ggtgattgag ctgacttata
     4501 actggggtgt ggataaatat gagctaggca cggcttatgg ccatattgcg ttaggcgtag
     4561 atgatattta caaaaccatt gaagatgtgc gtgcggcagg cggtaaaatt acccgtgagc
     4621 cgggcccggt attgggtgga acaactgtga ttgcctttgc tgaagatccg gacggttata
     4681 aaatcgaatt tattgagaat aaagatgcga aagcagcgtt aggaaactaa tttttgcgaa
     4741 tatgaatatt caagcggtcg gatttgtaaa aaattttgca aatcagaccg cttgtatttt
     4801 aggttgatag ataaatggaa cagacaacgg aacaaataga ttataaatta ttaaagcatc
     4861 gttttcgtgg ctatttaccg gttgtgattg atgtggaaac agcaggctta aatgcccaaa
     4921 ctgatgcctt attggaattg gcggcaatta ctttaaaaat ggacgagcaa ggttatctag
     4981 tgccggatca acaatgccat tttcatattg cgccatttga aggggcgaat attaaccctg
     5041 agtcattaaa attcaacggc attgatattg acaaccctct aagaggagcg gttgcggaga
     5101 atattgcgat tccggaaatg ttcaaaatgg ttcgcaaagc aatgaaggag caaggctgcc
     5161 aacgggcggt gattgttgcc cacaatgcag cgttcgatca gggcttttta caggctgcga
     5221 ttaaacgaat taatgccaag cgagatccct ttcacccttt cgcgatgttt gacaccgcca
     5281 cccttgccgg ttttatgtat gggcaaaccg tgttggtaaa agcctgccag gtggcaaaaa
     5341 tcccgtttga cggtaagcaa gcgcattctg cgttgtatga taccgagcgc acggcagaac
     5401 ttttctgtgc aatggtcaat cgtttaaaag atttaggtgg tttcccacca ttatcggatt
     5461 aacgttattc atttcattta ttaacacaat ttctcgtatt gtttctataa attatcctgg
     5521 gagtgatgat agttagtggc aagtaccatt ctgcaataaa tccagccctg tagagttgta
     5581 gaaaaaataa aaaactatct atcttcagtt aagattacaa gcggttggtt ttttaaccaa
     5641 atttggcaaa aatgtgacaa aatagaccgc ttgtattcaa taacaataga ctattttttg
     5701 gctataagca ctttctgtat acccgctatt tcgaccctcc ttatttttta tctaatctaa
     5761 aaacaagcgt ttccaccttg aaatgattaa ca
//
LOCUS       DANLRK010000159         5788 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.159, whole genome shotgun sequence.
ACCESSION   DANLRK010000159 DANLRK010000000
VERSION     DANLRK010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5788
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.159"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            <1..492
                     /locus_tag="PXA33_002144"
     CDS             <1..492
                     /locus_tag="PXA33_002144"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012602.1,HMM:NF016750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDL5121445.1"
                     /translation="AAPREYKLLRAKETRQTCTYCAVGCGMIMYSLGGEAMNSKGKLI
                     HIEGDSDHPVSRGALCPKGAGALDYVNSDRRVHYPEVREAGSSEWKRISWHEAIERIA
                     RHMKNDRDENFVTHNEAGLEVNRWMTAGFLAGSACSNETGIVTQKFIRSLGMIFTDNQ
                     ASI"
     gene            541..2976
                     /gene="fdnG"
                     /locus_tag="PXA33_002145"
     CDS             541..2976
                     /gene="fdnG"
                     /locus_tag="PXA33_002145"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007243156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HDL5121446.1"
                     /translation="MTNHWVDIKNSDLVIVMGGNAAEAHPVGFRWAVEAKKQNGAKLM
                     VVDPRFNRSAAVADIYIPLRPGTDIAFLLGAVNYLLKNDKIQHEYVKHYTNATFLLNE
                     GFKFEDGLFTGYDEATRTYKDKSTWAYQLDEQGQPKRDMTMQDPRCVINMLKDHVSRY
                     TPEMVERITGTPQKDFLIFCEEIAKTSAPDKAATFLYALGWTQHTVGAQNIRSMAIIQ
                     LLCGNIGVSGGGVNALRGHSNVQGITDLGLFPHMLPGYIRLPTEADATLESYLNAIAP
                     KTLVAGQVNYGQNNPKFAVSMLKTFYGDKATKENEFGYHYLPKYPKGGTDHFRFIEQM
                     YQGKVNGYFCQGMNPIASYPNSQKLIKALSNLKYLVIFDPLITDTSEFWKNHGEFNNV
                     NPSEIQTEVFRLPTTCFAEEDGSIANSGRWLQWHWKAADAPHEAKPDVDILSEIRAEL
                     IHLYHKEGGKAPLEPLEAMTWNYMNPLEPKPEEIAKENNGYALEDLKDADGNIIVKKG
                     ELLSSFTQMRDDGTTSGGCWIYCGQWTPKGNQMANRDNSDPSGLGNTLGWAFSWPVNR
                     RILYNRASADLSGKPWNPKRQLVKWNGKNWNYVDVADYGTAPPNSPVTPFIMQPEGVS
                     GLFVRERMQDGPFPEHYEPFETPIGTNPLHPNVISSPVARILPSDKEDLGTSDEFPYV
                     GTTYRLTEHFHYWTKNALINVIAQPEQFVEIGEKLAEEKGIKNGDKVRVWSKRGEIKA
                     VAVVTKRIRSLVSDGKPIHTIGVPIHWGFAATTGAKKGYFANNLTVRTGEPNSNTPES
                     KCMLLNIEKIVGA"
     gene            2979..3893
                     /gene="fdxH"
                     /locus_tag="PXA33_002146"
     CDS             2979..3893
                     /gene="fdxH"
                     /locus_tag="PXA33_002146"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit beta"
                     /protein_id="HDL5121447.1"
                     /translation="MSTVQTQNIIKISATSALTPAPQARSHQVEVAKLIDVTTCIGCK
                     ACQVGCSEWNDIRSDVNANCVGVYDNPADLNAKAWTVMRFNEVEENDRLEWLIRKDGC
                     MHCAEPGCLKSCPSPGAIIQYANGIVDFQSDKCIGCGYCIAGCPFNIPRMNPDDNRVY
                     KCTLCVDRVNVGQEPACVKTCPTGAIRFGSKEEMKFYAEQRIADLKSRGYENAGLYDP
                     EGVGGTHVMYVLHHADKPELYSGLPKDPKIDTTVTLWKDVLKPVAAVALGGLALAEVA
                     HYITVGPNVEEDLEEHHHSENAEGGKHE"
     gene            3886..4563
                     /locus_tag="PXA33_002147"
     CDS             3886..4563
                     /locus_tag="PXA33_002147"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HDL5121448.1"
                     /translation="MSKKFEITNDTKIVRHKPLARVSHWFLVISFFMTMFTGVAFFFP
                     DFAWLTEILGTPQIARAVHPFTGIIMFIAFIIMALIYWHHNIPEKNDIRWAKGIVEVL
                     KGNEHKVSDNGKYNLGQKLLFWTLILAMFTLLATGIIMWRQFFSHYFSIPMLRIAILL
                     HSASAFMLFTGILVHMYMAFWVKGSIRGMVEGWVTVRWAKKHHPRWYREEVLPELERQ
                     VAEQNNK"
     gene            4619..5536
                     /gene="fdhE"
                     /locus_tag="PXA33_002148"
     CDS             4619..5536
                     /gene="fdhE"
                     /locus_tag="PXA33_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005622482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HDL5121449.1"
                     /translation="MSIKILPQDDIKQAASSFQQPELLFANPKNLYLRRAKRLRELAK
                     QNPFGEYMEFSANLVDIQLALLESQPIADYSQKITACVERTQGETPLNAQNFQRTDEW
                     RTLLLAIIEKFKPYANDTVLATIEVLEKAAKSELDTLADNLLNERYELVGADKAVFIW
                     AALSLYWVQLAQQLPRNSRAEIGHKHLCPVCNSAPVSSVIHFGEAQGLRYLHCSLCES
                     EWHVVRTKCTNCDETGKLDYWSLDTVEAAVKAESCGDCHSYLKVLYQDKDVNVEPVAD
                     DLATLFLDSEMEQKGLSRSGINPFLFQVE"
     gene            complement(5606..>5788)
                     /locus_tag="PXA33_002149"
     CDS             complement(5606..>5788)
                     /locus_tag="PXA33_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121450.1"
                     /translation="LKNKEKDEEVSNFSMEELRVGAQRPLDDSVIQYFKSQCRYAFMS
                     DREIIANRCEVKKIAD"
BASE COUNT         1705 a         1266 c         1325 g         1492 t
ORIGIN      
        1 gctgcaccgc gtgagtataa gctcttaaga gcaaaagaaa ctcgacaaac ctgtacttac
       61 tgcgcggtag gctgtggcat gattatgtac agcttaggcg gcgaagcaat gaacagcaaa
      121 ggaaagttaa ttcatattga gggtgattct gaccatccag taagccgtgg ggcgttatgt
      181 ccgaaaggtg caggggcgtt ggactatgtt aatagtgacc gccgtgtgca ttatccggaa
      241 gtgcgtgaag ccggttcaag tgagtggaaa cgcatttctt ggcacgaggc aattgaacgt
      301 attgcccgcc atatgaaaaa tgaccgtgat gagaacttcg tcacccacaa tgaagcaggg
      361 ctggaagtaa accgttggat gacggcaggt ttcttggcag gctcggcgtg tagtaacgaa
      421 accggtatcg tcacccagaa gtttatccgc tctctcggta tgatttttac cgacaaccaa
      481 gcgagtatct gacacggacc aacggtagca agtcttgctc catcatttgg tcgcggtgcg
      541 atgaccaacc actgggttga tattaaaaac tccgatttag tgatcgttat gggcggtaac
      601 gcagccgaag ctcaccctgt gggtttccgt tgggcagttg aagcgaaaaa acagaacggt
      661 gcgaagttaa tggtggtaga tccacgcttt aaccgtagtg ctgcggtggc ggatatttat
      721 ataccattgc gtccgggtac ggacattgcg ttcttgttag gtgcggtaaa ctacctgctt
      781 aaaaacgaca aaattcaaca cgagtatgtg aagcattaca ccaatgccac attcttactc
      841 aacgaagggt ttaaattcga agacgggctt ttcaccggct acgatgaagc cactcgtacc
      901 tataaagaca aatccacttg ggcttatcag ttagatgaac agggtcagcc gaaacgggat
      961 atgacaatgc aagatccacg ttgtgtgatc aatatgctca aagaccacgt ttcccgctat
     1021 acgcctgaaa tggtagaacg cattaccggt acgccacaaa aagatttcct gattttctgt
     1081 gaagagattg cgaaaacctc agcgccggat aaagccgcca ccttcctcta tgccttaggt
     1141 tggacacaac acaccgtggg cgcacaaaac attcgctcaa tggcgatcat tcagttgctg
     1201 tgcggtaaca ttggtgtctc cggcggtggg gtaaacgcac tgcgtggtca ctctaatgta
     1261 caagggatta ccgacctagg cttgttccca catatgttgc cgggctatat ccgcctgcca
     1321 accgaagcgg acgcaacctt agaaagctac ttaaatgcga ttgcaccgaa aaccttagtg
     1381 gcagggcaag taaactacgg gcaaaacaac ccgaaattcg ctgtgagtat gctcaaaacc
     1441 ttctacggcg ataaggcaac caaagagaac gagttcggct atcactattt accgaaatac
     1501 ccgaaaggcg gtacagatca cttccgcttt atcgaacaga tgtatcaagg caaagtgaac
     1561 ggctatttct gccaaggtat gaacccgatt gcctcttacc ctaactcgca gaagttaatt
     1621 aaagcgttga gcaatttgaa atatttagtc atttttgacc cgcttattac cgatacttcc
     1681 gagttctgga aaaatcacgg tgagttcaac aatgtgaacc cgtctgaaat tcaaaccgaa
     1741 gtgttccgct tgccgaccac ttgctttgcg gaagaagatg gttcaattgc gaactccggt
     1801 cgctggttac aatggcactg gaaagcagca gacgcaccac acgaggcaaa accggacgtg
     1861 gatattctgt ctgaaatccg tgccgaactg atccaccttt atcataaaga aggcggaaaa
     1921 gcaccgcttg aaccgttaga agcgatgacg tggaactata tgaacccgtt agaaccgaag
     1981 ccggaagaga tcgccaaaga gaacaacggt tatgcattgg aagatctgaa agatgcagac
     2041 ggcaacatca tcgtgaaaaa aggcgaacta ttatccagct ttacacaaat gcgtgatgac
     2101 ggtacaacct ccggtggttg ttggatctac tgcggtcaat ggactccgaa aggtaaccaa
     2161 atggcgaacc gtgataactc cgacccatca ggcttaggta acaccttagg ctgggcattc
     2221 tcgtggccgg taaaccgccg tattctatac aaccgtgcat cggcagattt aagcggtaaa
     2281 ccgtggaacc cgaaacgcca gctagttaaa tggaacggta aaaactggaa ctatgtggac
     2341 gttgccgatt acggtacagc accaccaaat agccctgtga caccatttat tatgcaaccg
     2401 gaaggggtat ccggcttgtt cgtgcgtgaa cgtatgcaag acggtccatt ccctgagcat
     2461 tatgaaccgt ttgaaacgcc aatcggcacg aacccgttac acccgaatgt aatcagcagt
     2521 ccggtggcac gcattctgcc gagcgataaa gaggatttag gcaccagcga tgagttccca
     2581 tatgtcggta caacttaccg cttaaccgaa cacttccact actggacgaa aaatgccctg
     2641 attaacgtca ttgctcaacc ggagcaattc gtggaaatcg gcgagaaact tgccgaagag
     2701 aaaggcatta aaaatggcga caaagtacgt gtttggtcta aacgtggcga aattaaagcc
     2761 gttgccgtgg tgactaaacg tatccgttca ttggtctctg acggcaaacc aatccacact
     2821 atcggtgtgc cgatccactg gggctttgcg gctacaaccg gtgcgaaaaa aggctatttt
     2881 gccaataact taaccgtgcg aacaggtgaa ccaaactcaa atacacctga atcgaaatgt
     2941 atgttattaa acattgagaa aattgtgggg gcttaacaat gagtacagtt caaactcaaa
     3001 acattattaa aatctccgca acctcggcgc tgacaccggc gccgcaggct cgttcgcacc
     3061 aagtggaagt agcgaaactg atcgatgtca ccacttgtat cggctgtaaa gcctgtcaag
     3121 tgggctgttc agaatggaat gacattcgct ccgatgtaaa tgcaaactgt gtcggcgtgt
     3181 acgacaaccc tgcggatcta aatgcgaaag cgtggacagt aatgcgcttt aacgaagtag
     3241 aagaaaacga ccgtttagaa tggttgatcc gtaaagacgg ctgtatgcac tgtgccgagc
     3301 cgggttgctt gaaatcttgc ccgtcaccgg gtgcgattat ccaatacgcc aacggtattg
     3361 ttgatttcca atccgacaaa tgtatcggtt gtggctactg tatcgcaggt tgtccgttca
     3421 acattcctcg tatgaatccg gacgataacc gtgtttacaa atgcaccctg tgtgtggatc
     3481 gtgtcaatgt tggtcaagaa ccggcttgcg tgaaaacctg cccgaccggt gcaatccgct
     3541 tcggtagcaa agaagagatg aagttctatg cagaacaacg cattgctgat ctgaaatctc
     3601 gtggctatga aaatgccggc ttgtacgacc ctgaaggcgt tggcggtacg cacgtaatgt
     3661 atgtattaca tcatgccgac aaaccggagc tttattcagg cttaccgaaa gatccgaaga
     3721 ttgataccac agtaacctta tggaaagacg ttttaaaacc tgtggcggca gtagcgttgg
     3781 gtggtttagc tttagcagaa gttgcacact atattaccgt tgggccaaac gttgaggaag
     3841 atcttgaaga acaccaccat agcgaaaatg cagaaggagg caaacatgag taagaaattt
     3901 gaaatcacga acgataccaa aatcgttcgt cataagccct tagctcgtgt tagccactgg
     3961 ttcttagtaa tctctttttt tatgacgatg tttaccggtg ttgccttctt cttccctgac
     4021 tttgcttggc ttaccgagat tttaggtact ccacagattg cccgtgcagt ccacccattt
     4081 accgggatta taatgtttat tgcatttatt attatggcgt taatatattg gcaccataat
     4141 atccctgaga aaaatgatat tcgttgggca aaaggcattg ttgaagtact aaaaggtaat
     4201 gagcataagg tttccgataa tggaaaatac aatttgggtc aaaaattact cttttggact
     4261 cttattcttg cgatgttcac tcttttagcc acagggatta ttatgtggag acaatttttc
     4321 tctcactact tctcgattcc tatgctaaga attgcaattt tacttcattc agcaagtgca
     4381 tttatgctat ttaccggaat tttagtacat atgtatatgg cattctgggt aaaaggctca
     4441 atcagaggta tggttgaggg ttgggttacc gttcgctggg caaaaaaaca ccacccaaga
     4501 tggtaccgtg aagaagtgct acctgaatta gaaagacaag tagctgagca aaataataaa
     4561 taacctcaag tgcggtatta ttaccgcact tttattttat tggtttaaaa agaatattat
     4621 gagtatcaaa atactacctc aagatgatat caaacaagca gctagttcat ttcagcaacc
     4681 cgagctctta tttgctaatc ccaaaaatct gtatttacgc cgcgctaagc ggctacgtga
     4741 attagccaag caaaacccat tcggtgaata tatggagttt tctgctaacc tcgtggacat
     4801 tcaattagca ttacttgaaa gccagccaat tgcagattat tctcaaaaaa taaccgcttg
     4861 tgtagagcgc acgcaaggag aaacaccgct aaatgcacag aattttcagc gtactgatga
     4921 atggcggaca ctactcttag ccattattga gaaattcaag ccttatgcaa atgatactgt
     4981 tcttgcaaca attgaagtat tggaaaaggc agctaagtca gagctagaca cacttgctga
     5041 taatttgcta aatgaacgat acgaacttgt gggcgcagat aaagctgtct ttatttgggc
     5101 tgctctctca ctttactggg tacaattagc tcaacaatta ccacgtaatt ctcgtgcaga
     5161 aataggccat aaacatttat gtccggtatg taactcagca cctgtaagca gtgtgattca
     5221 tttcggtgaa gctcaagggt tacgttatct acactgttct ttatgtgaaa gtgaatggca
     5281 tgtagtacgt acaaagtgta caaactgtga tgaaaccggc aaactggatt attggagctt
     5341 agatacagta gaagcagcag ttaaagctga aagttgtggc gactgccatt cctacttaaa
     5401 agtactctat caagataaag acgtgaatgt ggaacctgtt gctgatgatt tagcaactct
     5461 attcttagat tccgagatgg aacaaaaagg tctttcaaga agtgggataa atccattttt
     5521 atttcaagta gagtaactca tttacaacaa aaacggagca taacgctccg ttttttatta
     5581 catttaactc atacactata cttttttaat cagctatttt tttaacttca cagcggttag
     5641 caataatctc ccgatctgac ataaatgcgt atcggcattg agatttaaaa tattgaatca
     5701 cagaatcatc aagaggtcgt tgtgctccaa cacgtaattc ctccatagaa aaattactaa
     5761 cctcttcatc tttctcttta tttttcaa
//
LOCUS       DANLRK010000160         5757 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.160, whole genome shotgun sequence.
ACCESSION   DANLRK010000160 DANLRK010000000
VERSION     DANLRK010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5757
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.160"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            136..1254
                     /gene="malK"
                     /locus_tag="PXA33_002150"
     CDS             136..1254
                     /gene="malK"
                     /locus_tag="PXA33_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005552148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HDL5121451.1"
                     /translation="MANVSLRNVGKSYGDVHISKDINLEINEGEFVVFVGPSGCGKST
                     LLRMIAGLEDITTGELYIGGKLMNEVEPSKRGIGMVFQSYALYPHLDVAENMSFGLKL
                     AGVSKAERDQRVNQVAEILQLAHLLDRKPKALSGGQRQRVAIGRTLVSQPEVFLLDEP
                     LSNLDAALRVQMRVEISKLHKKLNRTMIYVTHDQVEAMTLADKIVVLNAGGIAQVGKP
                     LELYHYPANRFVAGFIGSPKMNFLPVKVIAVEPERVKIELPDANHHNFWIPVSGEGVN
                     VGDNLSLGIRPEHLVPSDQTQVTLHGIVQVVELLGNETQIHLEIPEIKQPTLIYRQND
                     VVLVNEGDDLNIGILPERCHLFKEDGTACKRLFVEKGV"
     gene            1352..2641
                     /locus_tag="PXA33_002151"
     CDS             1352..2641
                     /locus_tag="PXA33_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin"
                     /protein_id="HDL5121452.1"
                     /translation="MKKTVLAVAISGAMFAASASAVDFHGYARSGIGWTSGGGEQSAF
                     TVNGGGSKYRLGNESDTYAELKLGQELFKSGEKSIYFDTNLAYGGTLHNNDWTPTSPA
                     LRELNVQFKNFADSLPGATLWAGKRFYQRHDVHMNDFYYWDISGPGAGVENIDLGFGK
                     LSLAVTRDTERGGANTFGYTTVYKLDPSTGKIKLDKQRNKADVYNDIFDVRLAGIELW
                     KDGSLELGFDYGNAHAKKGAALEKGATKNGYMVTAEYTQGNFFGGFNKFTAQYATDSM
                     TSWNNGHAQGSKADNKGNMLRLINQGVVQASDKVEVMYALIYEKTKLDNKQGKTWYSA
                     GIRPMYKWNDTMSTLLEVGYDRIKDQATGKKNDLVKYTVAQQWQAGNSIWARPAIRVF
                     GTYARWNDKFNTKARTDAGYKAKDGEFITGVQFEAWW"
     gene            2722..3618
                     /gene="malM"
                     /locus_tag="PXA33_002152"
     CDS             2722..3618
                     /gene="malM"
                     /locus_tag="PXA33_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HDL5121453.1"
                     /translation="MKKTLLSTAIFFAHLLVVSPSIANGSAGKSIHINSAELAQIQWQ
                     DVPFSQTVKTDLSEQHKQAFTTAFAGIASPVAAYRIPANQGTLEIEIESPVMDKNVFV
                     PTAVVLDSQFNVASTYPSSEFKLLEERGLKGSRLGAELNLTPAMNQDYIYLLIYTTQQ
                     DLAKTTMMPHPAKTYAKATGKQPPAINDIEVAHSLKGQIHINVTGSNGTKFIGLPTEI
                     FSSNKAATPAAKTAIPLPIKENPVAVKTAVDKDTEAYFNQAVMKAIKAKDINKAMNLV
                     NEAEKLGLTSPRQIFLKNVSSN"
     gene            complement(3778..5646)
                     /locus_tag="PXA33_002153"
     CDS             complement(3778..5646)
                     /locus_tag="PXA33_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HDL5121454.1"
                     /translation="MSGLVSPELIKTVALLATSVTIVPLFKRLGLGSVLGYLVAGCLI
                     GPSGIGLFQDPAAVVHMAELGVVMFLFIIGLEMHPELLWAMRKAIFGRGFLQVAICGT
                     LLTLAGIYLLGFSKEVAFIAGMGFTLSSTAIVMQVLEDKGISSTPKGQRIVATLLFED
                     LAIVPLLASVAFLAPNQVDAESSTNWSAIGLSLVAVLILVASGKWLMNPIFRVISKAK
                     VREMMTAAALLVVLGSALLMEISGLSMAMGAFLAGVMLSESSFRHQLEADIEPFRGLL
                     LGLFFMGVGMSLDLSLVLNNWLWLLVIVAVYVIGKGLGIFIAASITRLTLNESIVRTT
                     IMAHGGEFAFVLFSAAATAGILTPDTHATLTAAVIVSMLLSPLLILIKQGVRKKKAKN
                     ESTNMTGVELPEDLEANVLVIGFGRFNQIVCQALLAKGLSVSVIDSNTDHIRAAARFG
                     FKLYYGDGARLDVLRASGIANANCVIVGVGNPERTEKIVELIKSEYPLVPVFARTYDR
                     QSAVDLVKKHHVDYHIRETFESALALSQAAIERLGSSEEEAKQIISYVRRLDAERFNE
                     ELLHGFSAEVIRKYWVPTPWVNPQHEGSALNEETADILEELGETVEVIKVDITDKN"
BASE COUNT         1835 a         1134 c         1203 g         1585 t
ORIGIN      
        1 tgatttcttc tttagataaa aaggcgacta ttcagcctaa aaaaaagtat tttaggatga
       61 tttcttcttt agataaaaag gcgactattc agcctaaata aaaccaacaa gcaaaagcag
      121 aaaagaggtt tagttatggc gaatgtatca ttacgtaacg tgggaaaatc gtatggtgat
      181 gttcatatct ctaaggatat taatttagaa attaacgaag gagaattcgt tgttttcgtt
      241 ggaccgtcag gttgtggcaa atctacgcta ttacgaatga tagcaggttt ggaagatatt
      301 acgacaggag aactctatat cggtggaaaa ctgatgaatg aagtagagcc ttcaaaacgt
      361 ggtatcggta tggtgttcca atcctatgct ctctatccac atttagacgt tgccgaaaat
      421 atgtcttttg gcttaaagct ggcaggtgta agtaaagcag agcgggatca acgagttaac
      481 caagttgccg aaatcttaca gcttgcccat ttattagatc gaaaacctaa agcactttcc
      541 ggcggacaac gccaacgggt ggctattggt agaacactgg tttcccaacc ggaagtattt
      601 ttactcgatg aaccactttc caaccttgat gccgcattgc gtgtacaaat gcgggtagaa
      661 atttccaaat tacataaaaa actaaaccgc acaatgattt atgttacgca cgatcaagtt
      721 gaagcaatga cattagcgga caaaatcgtg gtattaaatg ccggcggtat tgctcaagta
      781 ggtaaaccgt tagaacttta tcattacccg gcaaatcgct ttgtagcagg gtttatcggc
      841 tcgcctaaaa tgaacttttt accggtaaaa gtgattgcgg tagagccgga acgggtaaaa
      901 attgagttac ctgatgcgaa tcatcataat ttttggattc cggtttccgg cgaaggggtg
      961 aatgtgggag ataacttatc tttaggtatc cgcccggaac atttagttcc ttcggatcaa
     1021 acccaagtga ccttacacgg tattgtgcaa gttgtggaat tgctcggaaa tgaaacacaa
     1081 atccaccttg aaatccctga aatcaaacaa ccaaccttaa tttatcgtca aaacgatgtg
     1141 gtattagtta atgaaggcga cgatctgaat atcggtatat tgccggaacg ctgccattta
     1201 tttaaggaag acggtacagc ttgcaaacgt ttgtttgttg aaaaaggggt ataacaattt
     1261 cagtttatta ctgattttag agggtatttt caatcttcta atcaattaat aattaaccta
     1321 agtaaaatca aatcttaaga gaggttctat tatgaaaaaa acagtcctag cagttgcaat
     1381 cagtggtgca atgttcgcag ccagtgcatc agcggttgat ttccacggat atgcacgttc
     1441 aggtatcggt tggacatcgg ggggggggga gcaatccgca ttcacggtaa atggtggtgg
     1501 ttcaaaatac cgtttaggta acgaatctga tacctatgca gaattgaaat taggtcagga
     1561 attattcaaa agtggtgaga aatctatcta ctttgataca aaccttgctt acggcggtac
     1621 gcttcacaat aatgattgga caccaacaag tccagcacta cgtgaattaa atgtacagtt
     1681 caaaaacttt gcagatagtt taccaggtgc caccttatgg gctggtaaac gtttttacca
     1741 acgccatgat gtacatatga atgacttcta ttactgggat atttcaggtc cgggtgcagg
     1801 ggttgaaaat attgatttag gcttcggaaa actctcttta gcggttactc gtgatacaga
     1861 gcgtggaggg gcgaatactt ttggatatac tacagtttat aaattagatc ctagcacagg
     1921 taaaattaaa ttagataagc agcgtaataa agccgatgtt tacaacgata tttttgatgt
     1981 tcggttagcc ggtattgagc tttggaaaga tggttcgtta gagttaggct ttgattacgg
     2041 taatgcacat gctaagaaag gtgcagccct tgaaaaagga gcaaccaaaa acggttatat
     2101 ggtaacagcg gaatacactc aaggtaactt ctttggtggt ttcaataaat ttactgcaca
     2161 atatgcaacc gactctatga cttcttggaa taatgggcac gcacagggtt cgaaagcaga
     2221 taataaaggt aatatgcttc gtttaatcaa ccaaggggta gtgcaagcga gtgataaagt
     2281 tgaagtaatg tatgccttaa tctacgaaaa aacaaaacta gataataaac aaggtaaaac
     2341 ttggtactca gcaggtattc gcccaatgta caaatggaat gatacaatga gtacattatt
     2401 agaagttggt tatgatcgca ttaaagatca agcaacaggc aagaaaaatg acttggtaaa
     2461 atacaccgtt gcacaacaat ggcaggctgg taatagcatt tgggctcgcc ctgcaatccg
     2521 tgtgtttggt acttatgcac gttggaatga taaattcaac acgaaagctc gtaccgatgc
     2581 cggctataaa gccaaagacg gcgagtttat caccggtgtt caatttgaag cttggtggta
     2641 atcatcaaac ctttgtgtgg gtaggattac ctacccacta tttcagcact ttatcctatt
     2701 tcacgatttt taggggttaa tatgaaaaaa acgcttcttt cgacagcaat attcttcgcc
     2761 catcttttgg tggtgtctcc gagtatagca aatggctcgg caggtaagtc tattcatatt
     2821 aactcggctg aattagctca aattcaatgg caagatgtgc cattttcaca aaccgtaaaa
     2881 acagatctaa gcgagcaaca caaacaagca tttaccacag cgtttgccgg tattgccagc
     2941 ccggttgcag cttatcgtat tccggcaaat caaggcacat tggaaattga aatcgaaagc
     3001 ccggtgatgg acaaaaatgt ttttgtaccc acggcagtcg tgttggatag tcaatttaat
     3061 gtggcatcaa cttacccctc ttctgagttt aaattattgg aagagcgagg actcaaaggt
     3121 agccgtttag gggcagaact taatttaacg ccggcaatga accaagatta tatttacttg
     3181 ctcatttata ccacgcagca agatttagcc aaaaccacga tgatgccgca tccggcaaaa
     3241 acctatgcaa aagcaaccgg taaacaacca ccagcaatta acgatattga agtagcacac
     3301 agtttaaaag ggcaaattca catcaacgtt accggctcga acggcactaa atttattgga
     3361 ttaccgactg aaattttcag ctcaaacaaa gcagcaacgc cggcagctaa aacggctata
     3421 ccgttgccga taaaagaaaa tccagttgca gtaaaaacag cagtagataa agatacggaa
     3481 gcctatttta atcaggcggt aatgaaagcg attaaagcaa aagatattaa taaagcgatg
     3541 aatttggtta acgaggcaga aaaattaggg ctgacttctc cacgccaaat tttcttaaaa
     3601 aatgtaagtt caaattgatt tattgttttt ttcatagata ttctccttgt catcgtgcga
     3661 tggtttatta ccattaacac cctatcagat tatgccgata gggtgctttt ttacgagtga
     3721 ttatgcgata tgtataggaa ggcttatact tgaataagga ttcaaagtat aaattcttta
     3781 atttttatca gtgatatcca cttttataac ttctactgtc tcaccaagct cttctaaaat
     3841 atcagccgtt tcttcattaa gtgcactacc ttcgtgttgt ggatttaccc aaggtgttgg
     3901 tacccaatat ttgcggataa cttctgcgga gaaaccgtgt aataattcct cattaaagcg
     3961 ttctgcatct aatcggcgaa catagctgat aatttgtttt gcctcttcct cgctagaacc
     4021 taagcgttca atggcggctt ggcttaatgc gagggctgat tcaaaggttt ctcgaatgtg
     4081 ataatcaacg tggtgctttt tgactaaatc aaccgcactt tgtctatcat aggttctcgc
     4141 aaatacaggt actaaggggt attcagattt aatcagctct acgattttct ccgttctttc
     4201 cggattgcct acgccaacaa ttacgcagtt tgcattggcg atgccagatg ccctaagtac
     4261 atctagtctc gctccatcac catagtaaag tttaaaacca aaacgagcag cagcacgaat
     4321 atgatcagta tttgaatcaa taaccgatac acttagtcct ttggctaata aggcttggca
     4381 gactatttgg ttaaagcgtc caaaaccgat gactaataca ttggcttcta aatcttccgg
     4441 taattcaacc cctgtcatat tagtggattc atttttcgct tttttctttc tcactccttg
     4501 cttgattaaa atgagaagtg gggaaagcaa cattgagaca attaccgcag cggttaaggt
     4561 agcgtgagta tctggggtta aaatcccagc ggttgcggcg gctgagaata gcacaaaggc
     4621 aaattcacca ccgtgagcca taatggtagt tcgcacaata gattcattca gtgttaatcg
     4681 ggtgatagag gcagcaatga agattccaag gcctttaccg ataacataaa ctgctacaat
     4741 caccaataac catagccaat tattgaggac gaggctgagg tctaatgaca ttcctacgcc
     4801 cataaagaat aagcctaaaa gtaaaccacg gaagggttca atatcagctt ctagttgatg
     4861 gcggaaagaa gactccgaaa gcattactcc ggcaaggaat gcccccattg ccattgaaag
     4921 cccactgatt tccattaaaa gagcggagcc taataccaca agtaaggcag cggcagtcat
     4981 catttctcgt actttggctt tagaaataac acggaaaata gggttcatca gccattttcc
     5041 actcgcaacc aaaataagaa cggcaactag gctaagacca atagcagacc aattagtact
     5101 ggattcagca tctacttggt taggagcaag aaaggcaacc gaggctaata atggcacaat
     5161 cgctaaatct tcaaatagta aagttgcaac aatacgttgc ccttttggcg tggaacttat
     5221 ccctttatct tccagcactt gcattacaat ggcagtagaa gatagcgtaa aacccatacc
     5281 ggcaataaat gccacttctt tggaaaaacc taataagtaa atgccggcta aggttaataa
     5341 agtaccgcaa atcgcaactt gtaagaaacc tcgcccgaag atggctttac gcatcgccca
     5401 aagcagttcc gggtgcattt ccaaaccaat aataaacaaa aacataacca cgccaagttc
     5461 tgccatatgc acaacagcgg cagggtcttg gaacaatcca atgcccgatg gcccgattaa
     5521 acatcctgcg actaaataac ccaatacgct tcctaaacca aggcgtttaa atagcggtac
     5581 aatagtgacg ctggttgcaa gtaatgcgac tgttttaatt aattccggac taactaatcc
     5641 tgacatagaa ttcccacctg tataatataa ataagattaa tgatgttttt gtgctaaata
     5701 atctaaatgc tggcggcttt gaaacggcac aatacataac tcaccgtttg gtgaaat
//
LOCUS       DANLRK010000161         5660 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.161, whole genome shotgun sequence.
ACCESSION   DANLRK010000161 DANLRK010000000
VERSION     DANLRK010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5660
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.161"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(37..573)
                     /gene="hpt"
                     /locus_tag="PXA33_002154"
     CDS             complement(37..573)
                     /gene="hpt"
                     /locus_tag="PXA33_002154"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HDL5121455.1"
                     /translation="MEKHHIETLHSKEEVLNRINELAKEINQHYHQKQCQNLVVVGLL
                     RGSFMFMADLVRKLELPVEVDFLTASSYGSSTESSRDVKILKDLDGEIQGKDVLIVED
                     IIDTGFTLSKVGEILKLREPNSLAICTLLDKPCRREVDVPVKWVGFEIPDEFVVGYGI
                     DYAQKYRHLDYIAKVVIE"
     gene            complement(612..1961)
                     /gene="pmbA"
                     /locus_tag="PXA33_002155"
     CDS             complement(612..1961)
                     /gene="pmbA"
                     /locus_tag="PXA33_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HDL5121456.1"
                     /translation="MSITDKQTLLQQEQELRQAVEFALNFAKQAGAEAEVGVTKVAGL
                     SVSSRLEQVENIEFNNDGSLGISVYLGKRKGNASTSDLQPESIQKTVEAALAIAKYTS
                     EDACTGLADKELLAFEAEDLELYHQADISVDQAVELAIEAEHYALNADEKIVNSDGAT
                     FNSHSGVRVYGNTHGMLQSYLSSRYSLSCSVISAYEDQLERDYEYTIFREFDKLQAPK
                     WVGEQAAIKAVDRLNPQRIKTGEMSVIFYNDVATGLVGHLAGAISGGALYRKSSFLLD
                     KLGEQILPEWFAISERPHLLRQLASSAFDSEGVITQNREIITDGVLQTYLMTSYSGRK
                     MGLKSTGHAGGIHNWLVKPNRQGGLNTLLKEMGTGLLVTEMLGSAINSVTGEYSRGAA
                     GFWVENGEIQYPVAEITIAGQLQDMYKNLVAVGDDIEHRSNIQTGSILLEKMMVSGE"
     gene            2060..2590
                     /locus_tag="PXA33_002156"
     CDS             2060..2590
                     /locus_tag="PXA33_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HDL5121457.1"
                     /translation="MAKKRSKNEIDWTDEEEEIIWVSKSEIKRDSEHLKKIGAELIEL
                     TPQNLEKIPLDDDLKEAIRQAQGFKMEARRRQIQFIGKLLRNRDQEPIQEALDKVKNR
                     HNQQQALLHKLELVRNQLIAMGDASLDNLLTEYPQLDRQHLRNLIRGAIKEREANKPA
                     KNYREIYQYLKTEIVE"
     gene            2596..2967
                     /locus_tag="PXA33_002157"
     CDS             2596..2967
                     /locus_tag="PXA33_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HDL5121458.1"
                     /translation="MEYLSTILKAHIGLAYISLILLLTRGFLSSKMVDWRQYKILRIA
                     PHAVDTFLLITGIAAVAIFLAYDFFTLAQFSWLLPKLLFLVLYIVFATKAFKKGQPFS
                     LKFYLLAVVSFLLMMLTATFH"
     gene            2980..3693
                     /locus_tag="PXA33_002158"
     CDS             2980..3693
                     /locus_tag="PXA33_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HDL5121459.1"
                     /translation="MIGPFVNAGAIILGGVLGACLGSHLPERIRTTLPLVFGLCCFGL
                     GMMLTASVKYMSAVALALILGTIIGELIYLENRIGKVAGLARGLIDRLFPPVHSLSQE
                     EFLEKFIALLVLFCVSGTGIFGAIKEGMTGDPSILYIKSILDFFTAMIFAASLGYAIA
                     AIAVPLIIVQGILILLATVIMPLTTANMMADFSATGGLLLFATGFRICGIKNFPVANM
                     LPALLLAMPMSYFWEWLVH"
     gene            3696..4259
                     /locus_tag="PXA33_002159"
     CDS             3696..4259
                     /locus_tag="PXA33_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HDL5121460.1"
                     /translation="MKKRIIVGISGASGFQYGYKVLELLKPFSEIETHLVISKGAELT
                     RTLETDYTREALHDLADQIHSLNNVGASIASGSFKTEGMVIAPCSMRTLAAIANGLSD
                     NLLTRAADVVLKERRKLVLMVREAPLNLAHIDNMRKVTEMGGIIFPPIPAFYQKPTSL
                     DEMVTHSVSHALSLLDIDIPNTPHWGE"
     gene            4322..4762
                     /locus_tag="PXA33_002160"
     CDS             4322..4762
                     /locus_tag="PXA33_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HDL5121461.1"
                     /translation="MHNPIPTHILDFIQTNHVVNIAAHTEQDFWAANCFYAFDQENVR
                     LIILTAKKTRHAGLMLENPHIVGTICGQIEKINEIEGVQFSATAECLEDKQAEKAALQ
                     IYYQKHPLARLKPSDVWVLSFNTIKHTGNKLMFAKKTIWERNII"
     gene            complement(4766..5641)
                     /locus_tag="PXA33_002161"
     CDS             complement(4766..5641)
                     /locus_tag="PXA33_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL5121462.1"
                     /translation="MMKTILIDEANQIKGFSHLYRLRSDLMLKYTEISVTETVEQEPA
                     EFEAGLRLVILLSGRSEIDFNSEVFELDASLKPQAAFLPLNRAEMGRKIFRKGQLQKE
                     LVIFLYPKWLIESQLNLASYHHFKQHLKAHQIILTQSMLKLIHDIVSEQEMQPFGLLE
                     KEGNLLALLASAFSQLTQAPRLKKDRVQQLTELLESGKFDGWSLKQIAIYFHTNVTTL
                     QNEFQHYHKTSIFAYLRRSKLEQAYKLLLQGQSITQAAEFAGYANSENFSTAFKRTFG
                     ITPSQIKRDKAKGII"
BASE COUNT         1632 a         1195 c         1061 g         1772 t
ORIGIN      
        1 attagtcgtg aaaatctaag tagtgattga gtttttttat tcaatcacta ctttagcaat
       61 ataatctaaa tgacgatatt tttgggcata atcaatccca taacccacca caaattcatc
      121 ggggatctca aatcccaccc attttacggg cacatcaact tcgcggcggc aaggtttatc
      181 taataaagta caaatagcaa gtgaatttgg ctcacgtaac ttgagaatct cccctacctt
      241 gcttaaggtg aagccagtat caataatatc ttctacaatc aatacatctt taccttgaat
      301 ttcgccatct aaatctttta gaattttgac atcacggctg gattccgtgc tgctgccata
      361 gcttgatgcg gttagaaaat ccacttctac aggtaactct agctttctta ctaaatctgc
      421 cataaacata aacgagcctc gcaataaacc aaccactaca aggttttggc actgtttttg
      481 gtgatagtgt tgattgattt cttttgctaa ttcattgatt cggtttaata cttcttcttt
      541 tgaatgtagc gtttcgatat gatgtttttc cattgatttc tccaaataaa attgattaca
      601 agcggccgtt tttactcgcc tgaaaccatc attttttcta ataagattga acctgtttgg
      661 atatttgagc ggtgttcaat atcatcgcct accgccacta aatttttata catatcttgc
      721 agttgccctg caatggtaat ttccgctaca gggtattgaa tttcaccgtt ttctacccaa
      781 aatcctgctg cacctcgaga gtattcccct gttacgctat taatcgcaga acctagcatt
      841 tccgttacta ataatcctgt tcccatttcc ttaagtaacg tatttaaacc accttgacga
      901 tttggtttaa ctaaccagtt atgaatacca cctgcgtgtc ctgtactttt taaccccatt
      961 ttacgccctg aatagctagt cattaaatac gtttgtagca cgccatcagt aataatttca
     1021 cggttttggg taattacacc ttcactatca aacgcagaag aggctaattg acgcaacaaa
     1081 tgcggacgct ctgaaatcgc aaaccattca ggcagaatct gttcgcctaa tttatctaat
     1141 aagaaacttg atttgcggta taatgcaccg ccactaatag ctccggccaa atgccccact
     1201 aaaccggttg ccacatcgtt atagaaaatc acagacattt cgccggtctt aattcgttgc
     1261 ggattcaatc tatctaccgc tttaattgca gcttgctccc ctacccattt cggtgcttgt
     1321 aatttatcaa actcacggaa aatggtgtat tcataatcac gctcaagctg atcttcataa
     1381 gctgaaatca cgctacaaga gagtgaatat cggctcgaca ggtaactctg caacattccg
     1441 tgcgtattgc cataaacacg cacgccactg tgagaattga aagttgcccc gtcactattc
     1501 acaatttttt cgtctgcatt taaggcataa tgctccgctt caatcgccaa ttcgactgct
     1561 tgatcaactg aaatatccgc ttggtggtac aattccaaat cttctgcctc aaaggcaagc
     1621 aattctttat cagcaagccc ggtacaagca tcttctgagg tatattttgc aatcgcaaga
     1681 gctgcttcaa ccgttttttg aatcgactct ggctgaagat cggaggtgga agcattacct
     1741 ttgcgtttgc ctaaataaac cgaaataccg agagaaccat cgttattgaa ctcgatgttt
     1801 tcaacttgct ctaaacggct tgaaaccgaa agcccggcaa ctttagtcac gcctacttcc
     1861 gcctctgccc cagcctgttt tgcaaaattt aaggcaaatt cgaccgcttg tcgtagctcc
     1921 tgttcttgtt gtaacaaggt ttgtttatct gtaattgaca ttttgttcca ttgattgatt
     1981 gcattgatga atatattcta actcaaattt ctgttagaat acggcatttt ttagagttta
     2041 tgtaatgaaa ggataaagaa tggctaaaaa acggagcaaa aacgaaattg actggacaga
     2101 tgaggaagaa gaaatcatct gggtcagcaa aagtgagata aaacgagatt ctgaacactt
     2161 aaaaaaaatt ggggctgaat tgatcgaatt gaccccacaa aatttggaaa aaattccact
     2221 agacgatgat ttaaaagagg caattcgcca agctcaaggc tttaaaatgg aggctcgccg
     2281 ccgtcaaatt caatttatcg gcaaattatt acgcaaccgt gaccaggagc cgattcaaga
     2341 agccttagat aaagtgaaaa atcgccataa tcagcaacaa gccctattac ataaattgga
     2401 attagtgcgt aatcaactga ttgcaatggg cgatgcatca ttagataatt tattgacgga
     2461 atatcctcaa ttagaccgcc aacatttgcg taatttaatt cgtggggcaa ttaaagagcg
     2521 tgaagccaat aaaccggcaa aaaattaccg tgaaatttat caatatttaa aaactgaaat
     2581 cgtggagtga ttattatgga gtacctatca accattctta aagctcatat cggcttagct
     2641 tatattagtt taattttatt attaacccgt ggatttttat cgtcaaagat ggtggattgg
     2701 cgacaatata aaattttgcg cattgcccca cacgcagttg ataccttctt acttatcacc
     2761 ggtattgctg ctgtcgctat tttcttagct tatgattttt tcaccttagc acaattcagt
     2821 tggttgctac caaagttact cttcttagta ctttatattg tatttgcgac aaaagcgttc
     2881 aaaaaaggac aaccgttttc gctcaaattt tatctgttag ccgtagtctc attcttatta
     2941 atgatgctaa ctgccacttt tcactaaata agggaaacta tgatcgggcc ttttgtgaat
     3001 gccggtgcaa ttattttagg gggcgtgctg ggtgcttgtt tagggagtca cctgcctgaa
     3061 cgtattcgca ctacgttgcc gctggtattc ggcttatgct gtttcggctt aggtatgatg
     3121 ctgactgcct cagtaaaata tatgtctgcg gttgccttag cattgatttt aggcacgatc
     3181 atcggtgaac tgatttattt agaaaacaga atcggtaaag ttgccggttt agctcgtggt
     3241 ttaattgacc gcctattccc accggtgcat agtctttccc aagaagagtt tttagagaaa
     3301 tttattgctc tgcttgtact gttttgtgtg agcggtaccg gcatttttgg tgcaataaag
     3361 gaaggaatga caggcgatcc aagtattctt tacattaaat cgattctaga tttcttcacc
     3421 gcaatgattt tcgctgcttc gttaggctat gccattgcgg cgattgcagt gccgttgatt
     3481 attgtgcaag ggattttaat tctactggca accgtgatta tgcctttaac cactgccaat
     3541 atgatggcag atttctccgc aaccggcggt ttactgctat ttgcaaccgg ctttcgcatt
     3601 tgtggaatta aaaactttcc ggtcgcaaat atgctaccag cactactcct tgcgatgccg
     3661 atgtcttatt tctgggaatg gttggtacat taatgatgaa aaaacgtatt attgtcggca
     3721 ttagtggggc aagtggtttt caatatggct ataaagtgct tgaactgctg aaaccgtttt
     3781 cagaaattga aacccacctt gtaatcagca aaggagctga actcactcgt actctcgaaa
     3841 cggattatac ccgagaagct cttcatgatt tagctgatca aatacattcg ctaaacaatg
     3901 tcggagcaag cattgccagt ggttcattta aaaccgaagg tatggtcatc gccccgtgtt
     3961 ctatgcgaac ccttgccgct attgccaacg gtttgagcga taatttgctg acccgtgcgg
     4021 cagatgtggt gttgaaagaa cggcgtaaat tggtattaat ggtgagagaa gctccgctca
     4081 accttgctca tattgacaat atgcgaaaag tcacagaaat gggcggcatt attttcccac
     4141 ccattccggc gttttaccaa aagccaacca gccttgacga gatggtcacc cacagcgtaa
     4201 gccatgccct ttccctgttg gatattgaca ttccaaatac cccacattgg ggagaataat
     4261 tcggcttatc ttttgctttt tgaaatgcta gaatacctga taaaaactat caggtatttt
     4321 tatgcacaat ccaatcccga cccatatttt agatttcatt caaactaacc acgttgtcaa
     4381 tattgcagct cataccgaac aggatttttg ggcagcaaat tgcttttatg cctttgacca
     4441 agaaaatgtt cgcttgatta tcttaaccgc caagaaaacc agacacgctg gcttgatgct
     4501 tgaaaaccca catattgtcg gcacaatttg tgggcaaatt gagaagatca acgaaattga
     4561 aggtgtccaa ttctccgcta ccgccgaatg tttagaagat aaacaagccg aaaaagccgc
     4621 tttgcaaatt tattaccaaa aacacccgct tgcacggctt aagcctagcg atgtttgggt
     4681 gttatcattc aataccatca agcacaccgg taataaactt atgtttgcga aaaagacgat
     4741 ttgggagaga aatataattt gaagctcaaa taatgccttt agccttatcc cgtttgattt
     4801 ggcttggtgt tatcccaaaa gttcgcttaa atgccgttga aaaattctcc gaattggcat
     4861 atccggcaaa ttcagctgct tgtgtaatac tctgcccttg taatagtaat ttataggctt
     4921 gttctaattt agaacgcctt aaatacgcaa aaatagaggt tttgtggtaa tgctgaaatt
     4981 cattttgtaa tgtggtcaca ttggtatgga aatatattgc aatctgcttc aaactccagc
     5041 cgtcaaattt accgctctca agcaattctg ttaactgttg cacccgatct ttttttaacc
     5101 ggggagcctg tgtcagctga gaaaaagcac ttgctaataa agctaataga ttaccttctt
     5161 tctcaagtaa cccaaatggt tgcatttcct gctctgacac aatatcgtga ataagtttta
     5221 acatactctg agtcaaaata atttggtggg cttttaaatg ctgtttaaag tgatgatagc
     5281 ttgccaaatt taactggctt tctattagcc atttagggta gagaaagata acaagctctt
     5341 tttgaagttg ccctttccta aaaattttac gccccatttc agctctattt agcggtaaaa
     5401 atgcggcttg aggttttaaa cttgcatcta attcaaatac ttccgaatta aaatcaattt
     5461 ccgatcgacc actcaacaat ataaccaatc ttagtccggc ttcaaattcc gcaggttctt
     5521 gctctacggt ttcagtaact gaaatctctg tatatttcag cataagatct gaacgtagtc
     5581 ggtataaatg tgaaaaacct tttatttggt tagcttcatc aatcaagatt gttttcatca
     5641 ttaaaaactt tttttcgcat
//
LOCUS       DANLRK010000162         5638 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.162, whole genome shotgun sequence.
ACCESSION   DANLRK010000162 DANLRK010000000
VERSION     DANLRK010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5638
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.162"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            43..732
                     /locus_tag="PXA33_002162"
     CDS             43..732
                     /locus_tag="PXA33_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDL5121463.1"
                     /translation="MSTLSRDSILSQIQNGLIASCQPVDDGPMDKPEIVAAMAAASVI
                     GGAKGIRIEGVDNLKAARAAVNVPIIGIVKRDLPDSPVRITPFLEDIDALAEAGADII
                     AVDGTFRTRPVSIEEAVKRIHEKGCLAMADCSTLEEGLHCQQLGFDIVGSTMSGYTGG
                     EMPDEPDYQLVKDLKAAGCNVMAEGRYNTPELAKTAIEIGADYVTVGSALTRLEHIVS
                     WFADSVKSAQK"
     gene            742..1644
                     /locus_tag="PXA33_002163"
     CDS             742..1644
                     /locus_tag="PXA33_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HDL5121464.1"
                     /translation="MRCLAIDIGGTKIATAIVQHNQVSQRQQIQTPTDKPQADQAGSL
                     HQAIGEIMQHYQGQFDFVAVASTGIINKGVLTALNPKNLGGLAEFPLKQSIEKHTDKP
                     IALLNDVQAAVCAEYLNEDQQAVKNFVFITVSTGVGGGIILNGELQIGSNGIAGHIGH
                     TLADPNGPLCGCGRVGCVEAVAAGRAIEAVSSQWEKPCSPKEVFERFRQGNPQAVELV
                     QKSAKAIANLIADLKISLDIQKVVLGGSVGLAEGYLPQVSNYLAEMPSVYHCVLENAL
                     SGQDAGLIGAAWWAENQLNQGFTL"
     gene            1669..2547
                     /gene="nanA"
                     /locus_tag="PXA33_002164"
     CDS             1669..2547
                     /gene="nanA"
                     /locus_tag="PXA33_002164"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005718701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HDL5121465.1"
                     /translation="MKNLKGIFSALLVSFNEDGTINEKGLRQIIRHNIDKMKVDGLYV
                     GGSTGENFMLSTEEKKEIFRIAKDEAKDEIALIAQVGSVNLKEAVELGKYATELGYDS
                     LSAVTPFYYKFSFPEIKNFYETIIRETGNKMIVYSIPFLTGVNIGVSQFAELFENEKI
                     IGVKFTAGDFYLLERLRKAFPNHLIYAGFDEMMLPATVLGVDGAIGSTFNVNGIRARQ
                     IFELAQQGKIKEAFEIQNVTNDLIEGILGNGLYQTIKGLLELEGVQAGYCREPMTKEQ
                     TPAQKEVTKQLKAKFL"
     gene            complement(2608..2814)
                     /locus_tag="PXA33_002165"
     CDS             complement(2608..2814)
                     /locus_tag="PXA33_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121466.1"
                     /translation="MDNKQTNGFNDIIVKADITQNIWSTGFYDIGRELKSENTRQEKV
                     RLKYNGDTLHRGRKSRMASLIASR"
     gene            complement(2772..3410)
                     /locus_tag="PXA33_002166"
     CDS             complement(2772..3410)
                     /locus_tag="PXA33_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121467.1"
                     /translation="MKKILLLVLLLTLATQADEGNNTQVCAEQQHQIIAHYFKISQEK
                     LSARTLASACKTAPNNSTETLVSYAVIEPKYSKENYQNDENTPKWTLFVARVNDNAIL
                     QSWEQDMEIDASIDVNADSLKIDTARYILNDKVRAFGVRFNNAARGPSAPEYSANDHL
                     MLFVPEKEKLKLVFDYPMTFWSILDDKETKTETQTWYCKWITNRPTASMILL"
     gene            complement(3652..4371)
                     /locus_tag="PXA33_002167"
     CDS             complement(3652..4371)
                     /locus_tag="PXA33_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL5121468.1"
                     /translation="MSKPNIYDESFSERMKWIAKNSFKDNYSEFARAVGVAQASLSRW
                     VKGEADPSRSNLVKIAEVSNVSLEWLATGKESKPQSENTSIVAREFERLEKMADDIVS
                     MINSFRTINVSAGFGSFNEGVTKPDGQEPYADSLLQSLGVKAHNCGVFWANGTSMHPT
                     IADSDQMLVDFSKREPRGDDKIYLVQNGESVWVKRIRKEWDYIELISDNESYRPIQIT
                     AEEAQNLQIIGQVVHIGHSLV"
     gene            4548..4826
                     /locus_tag="PXA33_002168"
     CDS             4548..4826
                     /locus_tag="PXA33_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL5121469.1"
                     /translation="MSVLNDVKKTAQRDWHKADILAELRKKGWSLRSLAKAGNVSHNT
                     LKSVLDKPYPKMERLVANAIGVAPEVIWAGRTAERIERNRRPTLTNKF"
     gene            4871..>5638
                     /locus_tag="PXA33_002169"
     CDS             4871..>5638
                     /locus_tag="PXA33_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL5121470.1"
                     /translation="MSELSLKTHYSVAELLSFKLSSLPSAHKNVLEKAIRENWQSQKR
                     KGRGGGVEYELISLPTEVQQEIRTKLLKLLPAEISKGELSVVRQNIDLEQITDKQLST
                     ADARIMVVRWFLMQEVQLGLSRTKTLDQVIAAVASSEIPAEICKAIMAGNSKAGGKLK
                     LSKRTLHSWVLAYEAGENSAERLKQMIPLKTQKRAVPERCGWLQAFLPFYQTFSNVAL
                     TQAYAQFAMQYEGEDLPTESQVRYALKQLPDYVVQQGR"
BASE COUNT         1679 a         1146 c         1238 g         1575 t
ORIGIN      
        1 ctagtccatt gactaccttt tgaacataaa aaaggaactg ttatgtcaac actatctcgt
       61 gattctattt taagtcaaat tcaaaacggg ctgattgcct cctgccaacc ggttgatgac
      121 ggccccatgg ataaacctga aattgtcgcc gcaatggcag cagcctctgt gattggtggt
      181 gctaaaggca ttcgcattga aggtgtcgat aatttaaaag ccgctcgtgc ggcagtgaat
      241 gtgccgatta tcggcattgt aaaacgtgat ttgccagatt cccctgtgcg tattacgcca
      301 tttttagaag atattgatgc cttagccgaa gccggtgccg atattattgc ggtggacggc
      361 acattccgca cccgcccggt cagcattgaa gaggcggtaa aacgcattca cgaaaaaggt
      421 tgcttggcaa tggcggattg ctccaccctt gaagaaggtt tacattgcca acaattaggc
      481 tttgatattg tgggtagtac aatgtctggc tacaccggtg gcgaaatgcc tgatgagccg
      541 gactatcaat tagtgaaaga tttaaaagct gccggctgta atgttatggc ggaaggtcgc
      601 tacaacacgc ctgaactggc aaaaacggcg attgaaatcg gggcggatta cgttaccgtt
      661 ggatcggcat tgacccgctt agagcatatt gtgagctggt ttgccgactc tgtaaaatcg
      721 gctcaaaaat aggagctgct tatgcgttgt ttagcaatag acatcggtgg cacgaaaatt
      781 gccacggcaa ttgtgcaaca caatcaagtg tcacaacgcc aacaaattca gaccccgact
      841 gataaaccac aagctgatca ggcaggcagt ttgcatcaag cgattggtga gataatgcaa
      901 cattatcaag ggcaatttga ttttgtggca gttgcctcaa caggcattat caataaaggg
      961 gtgcttaccg cattaaatcc taaaaattta ggcggattgg cagaatttcc gttgaaacaa
     1021 agcattgaaa aacataccga taagccgatt gcattattaa atgacgtgca agcggcggtg
     1081 tgtgccgaat atttaaatga agaccaacaa gcggtcaaaa attttgtatt tattaccgtt
     1141 tccaccggcg taggcggcgg cattatttta aacggcgaac tgcaaatcgg cagtaacggc
     1201 attgcaggac atatcgggca tactctagcc gatccgaacg ggcctctctg tggttgtgga
     1261 cgagtcggct gtgtcgaagc agtcgcagca gggcgtgcaa tagaggcagt ttccagccaa
     1321 tgggaaaagc cttgttcacc gaaagaggtg ttcgagcgat tccgccaagg caacccacaa
     1381 gcggtcgaat tagtacaaaa atctgcaaaa gcgattgcta atttaattgc agatttaaaa
     1441 atcagtttag atatacaaaa agtggtgtta ggtggcagtg tgggcttagc ggaagggtat
     1501 ttaccgcagg tgtccaacta tttagctgaa atgccgagcg tatatcattg cgtattggaa
     1561 aatgctctat ccgggcaaga tgccggctta atcggtgcgg cttggtgggc ggaaaaccaa
     1621 cttaatcaag gttttactct ttaacaaatt ctaactccgg agatccaaat gaaaaactta
     1681 aaaggtattt ttagtgcgtt actcgtctct ttcaacgaag acggcaccat caacgaaaaa
     1741 ggcttacgcc aaattatccg ccacaacatc gacaaaatga aagtggacgg tttatatgtc
     1801 ggcggttcaa ccggtgaaaa ctttatgctt tctaccgaag agaaaaaaga aattttccgc
     1861 attgccaaag acgaagcgaa agatgaaatc gcccttatcg cccaagtcgg tagcgtaaac
     1921 ttaaaagagg cggtagaact gggtaaatat gccacagaat taggctacga cagcttatct
     1981 gcggtcacac cgttctacta caaattcagc ttcccggaaa tcaaaaattt ctacgaaacc
     2041 attattcgtg aaaccggcaa caaaatgatt gtttactcca ttccgttctt aaccggcgta
     2101 aacatcggtg tgagccaatt cgctgaatta tttgaaaacg aaaaaatcat cggtgtaaaa
     2161 ttcactgccg gcgatttcta cctgttagag cgtttacgca aagcgttccc gaaccactta
     2221 atctacgcag gtttcgatga aatgatgcta ccggcaacgg tattaggtgt ggacggtgca
     2281 atcggctcaa ccttcaacgt aaacggtatt cgtgcaagac aaatctttga attagcccaa
     2341 caaggcaaaa tcaaagaagc atttgaaatc caaaatgtaa ccaacgattt aatcgaaggc
     2401 attttaggca acggtttata ccaaaccatc aaaggcttat tggagttaga aggtgtgcaa
     2461 gcaggttact gccgtgagcc gatgacgaaa gaacaaactc cggcacaaaa agaagtgacc
     2521 aaacagttaa aagctaaatt cttgtaattt aagttgcaaa aggctaaacg aaaatcaccg
     2581 cttgtagtaa caagcggtga ttttttctca acgacttgca attaatgaag ccattctgct
     2641 ttttctacca cggtgtaacg tgtcaccatt atattttaat cgcacttttt cttggcgagt
     2701 attttctgat ttcaactctc tacctatatc ataaaaccct gtactccaga tattttgagt
     2761 aatatccgct tttacaataa tatcattgaa gccgttggtc tgtttgttat ccatttgcaa
     2821 taccaagttt gtgtttccgt tttggtttct ttatcatcta aaatagacca aaaagtcatc
     2881 gggtaatcaa aaacaagttt taacttttct ttttccggca caaataacat caaatgatca
     2941 tttgcagaat actcaggagc acttggacca cgagcagcat tattaaacct cacgccgaaa
     3001 gctcttactt tgtcgtttag aatataacgg gcggtatcaa ttttaagact atctgcattc
     3061 acatcgatag aggcatcgat ctccatatcc tgctcccacg attgcaatat cgcattatca
     3121 ttcacacgag caacaaaaag cgtccactta ggtgtgtttt cgtcgttttg ataattttct
     3181 ttgctgtatt taggctctat taccgcataa ctcactaagg tttccgttga attgttagga
     3241 gctgttttac aggcagaagc taaagtacga gcagaaagtt tctcttgaga aatcttaaag
     3301 taatgagcaa taatttgatg ttgttgctca gcacaaacct gtgtgttatt accttcatct
     3361 gcttgagttg caagcgtaag taaaagaact aagaggagta tctttttcat atagatttat
     3421 ccgtatagaa agataactca ttctatgggg tactgactct ttttgcatag agagaaagaa
     3481 aaaaatttgg tcggaaaaat aacggcgagc ctagcgaaaa caagggttcg ccgtaatttt
     3541 atgggggaag ctatgtaaaa aacaaaatga gcaggattag aaaaaaatga gcagtttttg
     3601 cgatattttt tgcataggtt tgatcggttt taaaactatt ttaaatctgt tttaaactaa
     3661 agagtgtccg atgtggacta cctgcccgat gatttggagg ttttgagctt cttcggcggt
     3721 aatctgtatt gggcggtagg actcgttatc gctaattaat tcaatatagt cccattcttt
     3781 tctaatccgt ttaacccata cgctttcccc attctgcaca agataaatct tatcatcacc
     3841 acgaggctct cgtttgctga aatctaccag catttggtca ctgtctgcaa tggtagggtg
     3901 cattgatgtt ccatttgccc aaaacacgcc gcagttgtgg gcttttacgc cgaggctttg
     3961 cagcaggctg tcggcgtaag gttcttgccc gtctggcttc gttacgccct cgttaaagct
     4021 gccaaagcct gctgaaacgt tgattgttct gaagctgtta atcattgaaa caatatcatc
     4081 agccattttt tctagtcgct caaactctct tgccacaata ctggtattct ctgattgtgg
     4141 tttgctttcc ttacctgttg ccagccattc aaggctcaca tttgacacct ctgctatttt
     4201 tacaagattt gaacgagatg gatctgcctc tccttttacc cacctagata gtgatgcttg
     4261 ggctaccccc actgctcttg caaactcact gtaattgtcc ttaaagctat tttttgcaat
     4321 ccatttcatt ctttctgaga agctctcatc gtaaatgttt ggtttactca tcttttacgt
     4381 ttccattaat aaagttttac tcgtaaaaca tactcataaa ccattgattt ttaagttatt
     4441 tatgaataaa gcaaaaatat tttagatgtt tttatgtttt acggttgatt tgtttaaagt
     4501 tttacggtta aatacacgca tcaaatagat aaaaagaggt gtatcgaatg agtgtattaa
     4561 acgatgttaa aaaaaccgca cagagagatt ggcataaagc cgatattctt gcggaacttc
     4621 gtaaaaaagg ttggtcactt cgttctttgg ctaaagcagg caatgttagc cataacacgc
     4681 tgaagtctgt tttagacaag ccttacccca aaatggaacg cttagttgca aatgccatag
     4741 gggttgcccc tgaagtgatt tgggctgggc gaacagcaga acgcattgaa cgtaatcggc
     4801 gaccaacttt aactaataag ttttaatcat aaatgatctt taacgtaaaa gatataggag
     4861 aagaacattt atgagcgaat taagtttaaa aacgcactat tcagtggctg aattgttaag
     4921 ttttaagtta tctagcctac caagtgctca taaaaatgtg cttgagaaag caataaggga
     4981 aaactggcaa tctcaaaaac gcaaaggcag aggcggcggg gttgaatatg aattaatcag
     5041 tttacccacc gaagtccaac aggaaatccg caccaagtta ctcaaacttc ttcctgctga
     5101 aatcagcaaa ggcgaattga gtgtagtgcg acaaaacatt gatttggagc agataaccga
     5161 taaacaactt tctacggcag atgctcgcat tatggtggtg cggtggttct tgatgcagga
     5221 agtgcagttg gggctaagcc gcacaaaaac gctagaccaa gtcattgcag cggtggcaag
     5281 cagtgaaatt cctgctgaaa tttgcaaagc gattatggcg ggcaatagta aagccggtgg
     5341 aaagttaaag ctatctaaac gcaccttgca tagttgggtg ctggcgtatg aggcaggcga
     5401 aaattcagcg gaacggttaa aacagatgat accgctgaaa acacaaaaac gtgccgtgcc
     5461 cgagcggtgt ggttggttgc aagcctttct gccgttttat caaaccttta gcaatgtggc
     5521 actcacacag gcttatgctc aatttgcaat gcagtatgag ggcgaagatt tacctacaga
     5581 gagccaagtt cggtatgcct tgaagcagtt gcccgattat gtggtgcaac aagggcgg
//
LOCUS       DANLRK010000163         5606 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.163, whole genome shotgun sequence.
ACCESSION   DANLRK010000163 DANLRK010000000
VERSION     DANLRK010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5606
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.163"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            233..991
                     /locus_tag="PXA33_002170"
     CDS             233..991
                     /locus_tag="PXA33_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03761 family integrating conjugative element
                     protein"
                     /protein_id="HDL5121471.1"
                     /translation="MTTPLDLQLGPLRSEITFTLHTQYAHKLWMGRPMIRNEEGKVTQ
                     SSIISVPNCFAMLTQIQRAASEDDPYADDYLIQFEESVINYRKEIQKLTNDIVTLYAK
                     MLPEGISIERCANISPISYPIYVNSQLGYQLLYLLGDFDSLCRSVMTAAHIAIINRAE
                     AQDWIEAGARLIRKCFGIVERYKNSGITRRDYQENNARYQAAVKRMGYTLSDAVLTGE
                     HRAEFAPFIKQNGIVEEEQPVETHITTQANESQE"
     gene            1000..1509
                     /locus_tag="PXA33_002171"
     CDS             1000..1509
                     /locus_tag="PXA33_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3158 family protein"
                     /protein_id="HDL5121472.1"
                     /translation="MNLISDEIYRQLVKDSGLNASLKQLFSHFDTVSDYAVLQEQIQQ
                     ARAYFMNAQDGMVQYVRSEFSPLAVYMIKDKASSSGGTFLRWRSMQNARTGGTVWQPI
                     VDDESVPVDVRQKVVAVEKDRILINMQISVFNHILRQLVDCTEKLKEVDNAVAKSGLY
                     PLKNPVCNP"
     gene            1615..1815
                     /locus_tag="PXA33_002172"
     CDS             1615..1815
                     /locus_tag="PXA33_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121473.1"
                     /translation="MANVKMKFSVEIDGEVIIQHEIEEQAEKRSYFSQERKLAKEIIN
                     KMNEFYSDRWIDAKPWIASDDK"
     gene            complement(1931..2134)
                     /locus_tag="PXA33_002173"
     CDS             complement(1931..2134)
                     /locus_tag="PXA33_002173"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121474.1"
                     /translation="MELRKFPHHIIFIEQAKYGLQNYLLFELLTRTGQAVYFKHPIFH
                     TPNKEMRIVFVQDHKFLSLWQNK"
     gene            2243..2761
                     /locus_tag="PXA33_002174"
     CDS             2243..2761
                     /locus_tag="PXA33_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemophilus-specific protein"
                     /protein_id="HDL5121475.1"
                     /translation="MRYIKYSLLLISTMIMSGCAELSAINDKVGDWAGQMNQTLYGSK
                     ESSVEQHNGRQVYIKTRNGKYVYQDSGTLQLKQNIDEVFLKVRREFGFRPTNADSDAR
                     KVYHTVQGTFYHVSGYFKSDVDNDGVDLGENYLEVIIEKGSKGTVNVEWKATGSTRWL
                     DSTKQRLLNTLK"
     gene            2922..4973
                     /locus_tag="PXA33_002175"
     CDS             2922..4973
                     /locus_tag="PXA33_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDL5121476.1"
                     /translation="MKLFICEKPSQARDLASVLGATSKGDGLLATNDKSIIVTWGFGH
                     LVEQYQPEDYDEVWKRWAFETLPIIPAQWKMAPKKESKKQYNVVIGLIKKASLVVIAT
                     DADREGEMIARELLDLAGYRGQIQRCWLSALDDASIRKALQTLKSGKETEALYYAGMG
                     RSRSDWLIGMNFSRLFTLMAQAKGYSGKPLSVGRVQSPTLALVVNRDREIANFVPKAH
                     YSLAVQLATASDETFVAGLSIPEQYLDESGLCLDSRVIQQAEAQIKQVGIAKVITVET
                     KREKKAPPLLYALSDLQGECNRLFGFGAQQVLDIAQSLYEEHKITTYPRTDCGYLPES
                     QLTEVPMVIRSLVAADSELQTLLPRLNLQQKSRAWDDKKITAHHGIIPTIKKADLSKL
                     SEEEMKVYDLIRRRYLAQFLPHLETDKTIATLQSSGHTLIARGNVIVSQGWRILFGKN
                     ADEDVGSDDEKQGLPALTANQQCRVTHSEVRTLQTKPPAHYTEGTLLEAMKNAARFVT
                     DPRLKQRLRETEGLGTEATRAGLIQGLIDKGFLIKKKKSLMASAEAIALIDSLPDLLK
                     NPGLTALWEQALNQIAEGSMTLDDFMQRQETFVRQLIGNCMQQGMSLGNIEIRKCPEC
                     GKPMRKINHAKGTFWGCTGYPDCQHKEADKKVKSTSNKSTKKNSSLNVLDQLNKLRSQ
                     L"
BASE COUNT         1822 a         1051 c         1147 g         1586 t
ORIGIN      
        1 tagaggggtt ctacagtgag taaaaaataa tcagtgtaga gggactctac aatgagcaaa
       61 aaacaatcag tgtgtaggga ctctacaatg agcaaaaaac aatcagtgtg tagggggtac
      121 acactgatta aaaaataaac agagtagaca gtgtctacaa aggttaaaaa aatagacaat
      181 ggaaagcccc tttccaaagg ctaaaaaaca atcaaataaa caaggagcat atatgaccac
      241 accattagac ttacaattag gtccacttcg tagtgagatc acgtttacgc tacataccca
      301 atatgcccat aaattatgga tggggagacc aatgattcgt aatgaggaag gcaaagtaac
      361 tcaatcctcc attatcagcg taccaaattg ctttgcaatg ctaacccaaa ttcaacgggc
      421 tgcatcagaa gatgatcctt atgctgatga ttaccttatt caattcgagg aaagtgtgat
      481 caattatcgg aaggaaattc aaaaactcac caacgatatt gtcacgctct acgccaaaat
      541 gttgccggag ggaatttcta ttgaacgctg tgccaatatt tccccgattt cttacccgat
      601 ttatgttaat tcacagttag gctatcagtt gctttactta cttggtgatt ttgacagtct
      661 ttgtcgctca gtaatgacag ctgctcatat tgccattatc aatcgtgcag aagctcaaga
      721 ttggattgaa gcaggtgcaa gattaattcg taaatgtttt gggattgttg aacgttataa
      781 aaattccggt attactcgcc gagactacca agaaaataat gctcgctatc aagctgctgt
      841 aaaacgaatg ggctatacat tgtcagatgc tgtacttacc ggtgagcatc gtgctgaatt
      901 tgctcctttc atcaagcaaa acggcattgt tgaagaagaa caacccgtag aaacccatat
      961 cacgacacaa gcaaatgaat ctcaagaata aggagtgata tgaacctcat ttctgatgaa
     1021 atttatcggc aattagtcaa agactccggg ttaaatgcat ctctgaaaca gctatttagc
     1081 cattttgata ctgtctcaga ctatgctgta ttgcaggagc aaatacagca agcacgggct
     1141 tattttatga atgcacagga tggtatggtt cagtatgttc gtagcgaatt ctctcccctt
     1201 gcggtctata tgattaaaga taaggcttct tcttccggtg gtacgttttt acgctggcgc
     1261 agtatgcaga atgccagaac cggtggcaca gtttggcaac cgattgtaga tgatgaatca
     1321 gtcccggtcg atgtgagaca aaaagttgtc gcagtggaaa aagacagaat tttgatcaat
     1381 atgcagattt ccgtgtttaa ccatattttg cgacagttgg ttgattgtac tgaaaaacta
     1441 aaagaagtgg ataatgcggt tgcaaaatca ggtttgtatc ccctgaaaaa tccagtttgt
     1501 aatccctgaa aaagtaaggt ttgtaaagcc tgcattaaaa ggccttttaa aggtaagggg
     1561 acaagccccc ttacctttca cgaagaaaat gcctttatta tttcggagta aacaatggca
     1621 aatgtaaaaa tgaaattcag tgttgaaata gatggtgaag ttatcattca acatgagatt
     1681 gaagagcaag ccgaaaaacg ctcttacttt tcgcaagagc gtaaattggc caaagagatt
     1741 ataaataaaa tgaatgaatt ttattctgac agatggattg atgcaaaacc ttggattgct
     1801 tctgacgata aataaaagca atgagcatca tcgattacag gtttcataaa ttgatttcgc
     1861 taaggtattt aacttaatag caataaaatc ttcctgagtg taggacgatt tatcccattc
     1921 tttaataagg ttatttgttt tgccataggg ataaaaattt atgatcttga acaaagacaa
     1981 ttctcatctc tttgttaggg gtatggaata tcggatgctt aaagtaaacc gcttgtcctg
     2041 ttcttgttaa tagttcaaag agtaagtaat tctgtaatcc gtatttagct tgctcaataa
     2101 agataatgtg gtgaggaaat tttcttaatt ccataatagt ccctagcatt ttctctctta
     2161 ctacctaaaa gaattttaag tagattttaa ccgcactttt gcaaaaaatg aatgcagata
     2221 acccgcttgt aaggagaatt ccatgcgtta catcaaatat agcttacttc tcatttctac
     2281 tatgattatg tcaggttgtg ccgaactttc tgcaattaat gataaggttg gagattgggc
     2341 aggacagatg aaccagactc tctatggttc taaagaaagc tcagttgaac agcataatgg
     2401 gcgacaggtt tatattaaaa ctcgaaatgg caagtatgtc tatcaagatt ccggcacatt
     2461 acagttgaaa cagaatattg atgaagtctt cctaaaagtt cgccgagaat ttggcttccg
     2521 tccaaccaat gcagatagcg atgcacgtaa ggtttatcat acggtacaag gtacctttta
     2581 ccacgtttcc ggttatttca aatctgatgt agataacgac ggcgtggatt taggtgaaaa
     2641 ctacttagag gttatcattg aaaaaggcag taaaggcacg gtgaatgttg aatggaaagc
     2701 cacaggtagt acaagatggc tagatagtac caaacaacgt ttacttaata cattaaaata
     2761 agaactcata tcgctcctag aacctcttcc cagtggaaac actgggattt ttttatcgtt
     2821 ctcatctctc aatttattgc attctacaag ataataagtc gttgaatgct atttaatcag
     2881 cacattacta ttgcctatcc gtattgattt tggaaaatag tatgaaactg tttatttgtg
     2941 aaaagccctc tcaagcccga gatcttgcaa gtgtattagg tgctacatcg aaaggggatg
     3001 gactattggc aactaatgat aagtctatta ttgtaacttg gggatttggt catcttgttg
     3061 agcaatacca acccgaggat tatgatgaag tttggaaacg ctgggcattt gaaacattgc
     3121 cgatcattcc tgctcaatgg aaaatggctc ctaaaaaaga gtccaagaaa caatacaatg
     3181 tagtcatcgg attaattaag aaagcctctt tggttgtgat tgcaaccgat gcagacagag
     3241 aaggtgaaat gattgctcgt gaactgttag atttagccgg ataccgagga caaatacagc
     3301 gttgctggct atcagctctc gatgatgcca gtatccgaaa agctctccaa acactcaaat
     3361 caggtaaaga aaccgaggca ctttactacg ctggtatggg acgtagccgc tcggactggc
     3421 tgattggaat gaacttctcc cgtctattta ccttaatggc ccaagcaaaa ggctattccg
     3481 gtaagccatt aagcgttggt cgagtgcaaa gcccaacact agctcttgtg gtcaatcgag
     3541 acagagagat tgcaaatttc gtgcctaaag ctcattactc cttagcagtt caattagcaa
     3601 cagcgtctga tgaaaccttt gttgccgggc tttcaatacc tgaacagtat ttagatgaat
     3661 cagggctttg ccttgatagt cgggtcatac aacaagctga agctcaaatt aaacaagtag
     3721 gcatagccaa agtgataacg gttgaaacca aacgagagaa aaaggcaccg ccgttgcttt
     3781 atgcattaag tgatttacaa ggggaatgta accgcttgtt tggttttggg gctcaacaag
     3841 tacttgatat tgctcaatca ctttatgagg agcataaaat tactacttat ccccgtaccg
     3901 attgcggcta tctacctgaa tctcagctta ctgaagtacc gatggtaatt cgttcattag
     3961 tcgccgctga ttcagagtta caaactctat tgcctcgatt aaatctacaa caaaaaagtc
     4021 gggcttggga tgataaaaaa attactgctc accacggtat tatccccact atcaaaaaag
     4081 cagatttaag caagttaagt gaggaggaga tgaaggtcta tgatctcatt cgccgtcgct
     4141 atctcgctca attcttaccg cacttagaaa cagataaaac tatagccacc ttgcagtcct
     4201 ctggacacac tctgattgct agaggaaatg tgatcgtttc tcaaggttgg cgtatcctgt
     4261 ttggtaaaaa tgcagatgaa gatgttggta gtgatgatga gaaacaaggc ttacctgcat
     4321 tgacggcaaa tcagcaatgc cgtgttacac attcagaagt ccggacatta caaaccaaac
     4381 caccggcaca ctatactgaa ggaacactac tcgaagcaat gaaaaatgca gctcgctttg
     4441 tgactgatcc aagacttaaa caacggttgc gtgaaacgga gggattgggt acagaggcaa
     4501 ctcgtgccgg attgattcaa gggctcattg acaaaggttt tttaattaag aagaaaaaat
     4561 ccctaatggc ttctgctgaa gcgattgccc ttattgatag cctaccggat ttattaaaaa
     4621 atcccggttt aacggctctt tgggaacagg cacttaatca aattgctgaa ggcagtatga
     4681 cccttgatga ctttatgcaa cgacaagaaa cctttgtacg acaacttatc ggaaattgta
     4741 tgcaacaagg tatgtcattg ggtaatattg aaattcgaaa atgcccggag tgtggtaagc
     4801 caatgcggaa aatcaatcac gctaaaggta cattctgggg ttgtacagga tatccggatt
     4861 gccaacataa agaagcagat aaaaaagtca aatcgacttc aaataaatct accaagaaaa
     4921 atagtagctt aaatgtgcta gatcagctga ataaattaag aagccagtta taaatctaag
     4981 cagattatgg tattatcaaa gtgcaaagaa cccgtttaat agattgtcgg ttatcaatct
     5041 ataccagccg aaatgtctcc cagcattctg aaagggaact ctgtaaaatt acaggaatgc
     5101 cacctaaaca ataaatggat aatgccagcc actttatccg ttgtattttg tcacatgcgt
     5161 gattgcaatg tcataatatt acccgacttg gggatattat gacattgcaa tcattgctca
     5221 cttttcttag cctgagccca caggtgaata aagggtgccg tttgtgtcct cgatgatgcc
     5281 acagatatgc cgattaaaga ccacaaggta atcgggtaaa ttacgaacga aaatatcaac
     5341 attgtcacga aggtgctgct attttcaata tagtaactta tattggtcta acttaaattt
     5401 catttattct tcaaaagtac aacaccgttg gtaagtgatt tatcaacggt gttgtcattt
     5461 ccaattctct aaaatcaaaa ttcaattctc aattaacttt atgattcaat atgactaaat
     5521 tatcctcaat ttaagacgct gtatataaca gcacttaata agtcgcataa ctcctgagaa
     5581 gcgacggcta tccttagagg tagttg
//
LOCUS       DANLRK010000164         5564 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.164, whole genome shotgun sequence.
ACCESSION   DANLRK010000164 DANLRK010000000
VERSION     DANLRK010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5564
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.164"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(80..955)
                     /locus_tag="PXA33_002176"
     CDS             complement(80..955)
                     /locus_tag="PXA33_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDL5121477.1"
                     /translation="MWIWFTLLAAVAQAGRNAFQKQLSVNVPVLGVTLARFFYAVPLA
                     SGYLYFLYQYDDNLNIPHFPPLFFVYAIGAGLAQILATSLMVQLFHLKNYAIGVGLAK
                     SEAVWAALLGVLFFGVTISGIGWLGVVLGGVAIFLMTGSGNLRQLSWKTLFLGIASGL
                     CFALTSLWVREASLQLNSHYLLSAAWVLFSVISFEAVILVIYLALLQKETLIKFFRFP
                     KLGMLTSLFSFLGSFGWFNAMSLNHVAFVKTLGQVEIFFILGISYFVFKERLKIQDFI
                     GLILVVIAAILVVLG"
     gene            1063..1899
                     /locus_tag="PXA33_002177"
     CDS             1063..1899
                     /locus_tag="PXA33_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HDL5121478.1"
                     /translation="MLPHIYIGTGGYADTDLIGTLYPFGTAKEDFLFVYSQHYDTLEI
                     NSTFHAPIGQKAFEGMVQKAEGRLNFSVKLHQDFSHQRTATLDQAQLFLNALAPFTAE
                     GLLANLFVQFPTQFERMPLNRRYLAELCQWFADYPLAIEFRHPSWHIPAVFDTFAKSS
                     NLIWCNVDYPQNIGLPAFHFFANQRTAYLRLHGRNPNWWKGQSAAERHDYRYSDEELK
                     GLAELLFSRRTEFDRLYLYFENTTKSHAFYNIQTLKGFLAEKGFQIKPVPENLLGQQS
                     LF"
     gene            complement(1974..4826)
                     /locus_tag="PXA33_002178"
     CDS             complement(1974..4826)
                     /locus_tag="PXA33_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HDL5121479.1"
                     /translation="MVQAVSFSHFFAIHYFYFFLHKTKGQCTAKPLFSILLFHLFPKN
                     SAKFLLFFYFLVLMVLYSNEVANGKLLITGGTNLNGTLSAEGTSEIILSGRPTPHAYD
                     KVANKEVLIEGDWINRSFNATIFAAKDNGKLEISRNVANINGNFNLTDNATAQIGFTQ
                     GTSQACIRSDRTGVATCNINATLSETDLNSWERTKVAGNVSLADNSTFSLGSKADLTG
                     SITAQESTKVQLNDGSIANLTGLSTTGIFNGANGSTVNLSGVWTTLTNTVVDVLNVAN
                     GSQIHLNTPTATKQNTLTANTLTGNGIFKFVADLTKTASDIVTIKDTASGQFSIEIST
                     NGSAHSAQKIKLFETTNEEDFSLNLANEVRSGEYRYHLLKEGNVYYLNPEKEEEPKIT
                     QKGPSETADPLPEYTGDIEHSEKGPGETADPLPEYTGDIEHSEKGSGETAEPLPKYNG
                     NAHLIASELTAQTNAVLGVTTVLDHVIHSEGTQKQNTLTANTLTGNGIFKFVADLTKT
                     ASDIVTIKDTASGQFSIEISTNGSAHSAQKIKLFETTNEEDFSLNLANEVHSGEYRYH
                     LLKEGNVYYLNPEKEEEPKITQKGPSETADPLPEYTGDIEYSEKGPGETADPLPEYTG
                     DIEHSEKGPGETADPLPEYTGDIEHSEKGSGETAEPLPKYNGNAHLIASELTAQTNAV
                     LGVTTALDHVIHSEGTQNWKVWSKTDYQTTEHRSAGYGFNQDANVIHLGLEKALDNQV
                     TLGMILSQSKARNNFDHYYSGKGRLTMLSMYAKKTWQNGVFVAIDTGFGKASNRLTYQ
                     ANTVKLDRSVFVTGLSIGKAWESANVNIIPSFSARYHHLSSAGNQLVDAKIETNAVDL
                     LALQAGLSINKTLELNGLQIKPEIGSYFVDASHGKLRTRFNNLQIEQQMGRYFKQEAG
                     ISANYRNINAGIQAGFLTGNTLNKQRYISFKVTYEW"
     gene            complement(4874..5551)
                     /locus_tag="PXA33_002179"
     CDS             complement(4874..5551)
                     /locus_tag="PXA33_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL5121480.1"
                     /translation="MNSIIRKEWFTASEVAGMGNLPEQPSNVTRRATKNNWLKRQIEG
                     KRGVAFEYHYSSLPISVQQELGFAQTAVKEEPRTLVAAQTADEMERVPFYNVQASAGF
                     GAFNSEVYAPDDYIGLSKRWLDVRGFYLSYLAFITASGDSMYPTISDGDMLLVNRATT
                     QPKDGKIYVFRQGEQLWVKRVQGIMGGIRLISDNKELYSPIDVIFDETLDFEVIGQVV
                     YIGHEMI"
BASE COUNT         1565 a         1193 c         1105 g         1701 t
ORIGIN      
        1 ccacggataa atgatttatt ggttttgcgt gggcttacgc ccaccctaca aagctagata
       61 aaaaacgtaa aaatccgatc tagcccagca ccaccaagat tgcggcaatc actactaaaa
      121 tcagccctat aaaatcttga attttaagcc gttctttaaa cacaaaatag gaaataccga
      181 gaataaagaa aatttccact tgtccaagcg tttttacaaa ggcaacgtgg ttcaggctca
      241 ttgcattgaa ccaaccgaat gagccgagaa atgaaaacag gctggtcaac atacctaatt
      301 ttggaaaacg aaagaattta atcagtgtct ctttttggag aagagctagg tatataacca
      361 atatcactgc ctcaaagcta atcacgctaa aaagtaccca cgccgctgaa agcaaatagt
      421 ggctgttgag ttgcaggctt gcctctcgca cccaaagtga tgtgagggcg aaacataaac
      481 cgctggctat gccgagaaag agtgttttcc acgaaagttg gcgtagatta ccgctgccgg
      541 tcattaagaa aatcgccact ccacctagta ccacgccaag ccagccaatg ccactgatgg
      601 ttacaccaaa aaataacacg cccagtaaag ccgcccatac tgcctcgctt ttcgccagcc
      661 cgacacctat tgcataattt tttaagtgaa aaagttgcac cattagcgaa gttgccagaa
      721 tttgagccaa cccagccccg atagcatata caaaaaacag cggtggaaaa tggggaatgt
      781 tgaggttatc gtcatattgg tataaaaaat agagataacc gcttgccaac ggcacagcat
      841 agaaaaagcg ggcgagcgtt acccccaata caggcacatt gacgcttaat tgcttttgaa
      901 aggcattgcg accggcttga gccacggctg ccagcagagt aaaccaaatc cacataatga
      961 tgatgaaata agaaaagata gccaaatctt actcttttat tctcagtttg caagcggtta
     1021 aaaaaccatt aaaatttgca aaaaattgaa agaataaaca caatgctccc tcatatttat
     1081 attggcaccg gcggctatgc cgacacagat ttaatcggca cgctttaccc attcggcact
     1141 gcaaaagagg attttctctt tgtctatagc caacattacg acacgctgga aattaacagc
     1201 acattccacg cccccatcgg gcagaaagcc tttgaaggaa tggtgcagaa agcggaaggt
     1261 cggctgaatt tttcggtgaa actgcatcaa gatttcagcc atcaacgcac ggcgacttta
     1321 gatcaagcac aattatttct taatgcgtta gctccgttca ccgctgaagg gttattggca
     1381 aatttatttg ttcagttccc aactcaattt gaacgtatgc cacttaaccg ccgttacctt
     1441 gccgagttgt gccaatggtt tgcggattat ccgttagcga ttgaatttcg ccacccaagc
     1501 tggcatattc cggcggtgtt tgataccttt gcgaaaagct ctaatctgat ttggtgtaat
     1561 gtggattacc cacaaaatat cggcttaccg gcatttcact tttttgccaa ccaacgtact
     1621 gcttatttac gcttacatgg tcgtaacccg aattggtgga aagggcagag tgcggcagaa
     1681 cgccacgatt accgctattc agacgaggaa ttaaaaggct tggcagaatt gctttttagc
     1741 cgcagaacgg aatttgacag gctctatctc tactttgaaa acaccactaa aagccacgcc
     1801 ttttataata ttcaaaccct aaaaggcttt ttggcggaaa aaggatttca aatcaaacct
     1861 gtgccggaga atttgcttgg gcagcagagt ttgttttgat gggtaattta cattgttgag
     1921 gagtaaataa agggccatgc catttagtaa tggatagccc ttaatctcaa tatttaccat
     1981 tcatatgtaa ctttgaacga gatataacgc tgtttattta gcgtattccc cgttaagaaa
     2041 cctgcctgaa ttcctgcgtt tatattgcga taattagcgg atatacctgc ttcttgttta
     2101 aaataacgcc ccatttgctg ctctatttgt aaattattga aacgtgttct taatttccca
     2161 tggcttgcat caacaaaata gctacctatt tccggtttta tttgcaggcc atttagttca
     2221 agcgttttat taattgacag accagcttga agagcgagaa gatcaacagc gtttgtttcg
     2281 atttttgcat ctacaagttg attacctgct gaagataaat gatgataacg ggcgctaaat
     2341 gatgggatta tattcacgtt tgcagattcc catgctttac cgatactaag ccctgttaca
     2401 aatactgaac gatctaattt tactgtattt gcttgataag ttaagcgatt gcttgctttc
     2461 ccaaaaccgg tatctatcgc aacaaaaaca ccattttgcc atgttttctt cgcatacatt
     2521 gatagcattg ttaagcgacc tttaccgcta taataatggt caaaattatt tctcgctttt
     2581 gattgagata atatcatacc aagtgtgacc tgattatcta aggctttctc taatcctaga
     2641 tggataacgt tcgcatcttg gttaaatcca tatccggcag agcgatgctc tgtcgtttga
     2701 taatctgttt tagaccatac tttccaattt tgggtccctt ccgaatgaat tacatgatct
     2761 aatgctgtag ttacacctaa tacagcatta gtttgcgcag ttaactcgga tgcgatcaaa
     2821 tgtgcatttc cattgtattt aggtaatggc tcagcggttt cacccgatcc tttttctgaa
     2881 tgctcaatgt ctcctgtgta ttcaggtaac ggatcagcag tttcacccgg ccctttttcc
     2941 gaatgctcaa tgtctcctgt gtattcaggt aacggatcag cagtttcacc cggccctttt
     3001 tcagaatact caatatcccc ggtgtattca ggtaatgggt cagcggtttc acttggccct
     3061 ttttgagtaa ttttaggttc ttcctctttc tctggattga ggtaatacac attcccttct
     3121 ttgagtaaat ggtagcgata ttcgcctgag tgcacttcat ttgctaaatt aagagaaaag
     3181 tcttcttcat ttgttgtttc aaataattta atcttttggg ctgaatgggc gctaccattt
     3241 gtgctaattt ctatcgaaaa ttgaccgctt gcggtgtctt taattgtgac tatatcgctc
     3301 gctgttttcg tcagatcagc gacaaattta aatatgccgt tccctgtgag ggtattagcg
     3361 gtaagcgtat tctgtttttg ggtcccttcc gaatgaatta catgatctaa tactgtagtt
     3421 acacctaata cagcattagt ttgcgcagtt aactcggatg cgatcaaatg tgcatttcca
     3481 ttgtatttag gtaatggctc agcggtttca cccgatcctt tttctgaatg ctcaatgtct
     3541 cctgtgtatt caggtaacgg atcagcagtt tcacccggcc ctttttcaga atgctcaata
     3601 tccccggtgt attcaggtaa tgggtcagcg gtttcacttg gccctttttg agtaatttta
     3661 ggttcttcct ctttctctgg attgaggtaa tacacattcc cttctttgag taaatggtag
     3721 cgatattcgc ctgagcgcac ttcatttgct aaattaagag aaaagtcttc ttcatttgtt
     3781 gtttcaaata atttaatctt ttgggctgaa tgggcgctac catttgtgct aatttctatc
     3841 gaaaattgac cgcttgcggt gtctttaatt gtgactatat cgctcgctgt tttcgtcaga
     3901 tcagcgacaa atttaaagat gccgttccct gtgagggtat tagcggtaag cgtattctgt
     3961 tttgttgcag ttggagtgtt taaatggatt tggctaccat ttgcgacatt caagacatct
     4021 acaacagtgt tggttaaggt tgtccatacc ccagaaagat taacggttga accattggcg
     4081 ccattaaata ttcctgttgt actcaagccg gttaaattag caatacttcc atcattaagt
     4141 tgaaccttag tggactcctg agctgtaatt gagccagtta agtctgcttt actgcctaac
     4201 gaaaatgttg aattgtcagc taaagagaca ttgccagcca ctttggttct ctcccagctg
     4261 tttaaatcag tctctgataa agtagcgtta atattacagg ttgctacgcc tgtgcggtca
     4321 gagcgaatac atgcttgtga ggtaccttgg gtaaaaccaa tttgagcggt agcattatct
     4381 gttaaattaa aattaccgtt aatattggca acattacggc tgatttcaag cttgccattg
     4441 tctttagctg caaagattgt tgcgttgaac gatcggttga tccagtcgcc ttcaatcagg
     4501 acttctttat tcgctacttt atcataggca tgaggtgttg gacggcccga taaaatgatt
     4561 tcgcttgtac cttctgcaga aagcgtaccg ttgaggttcg ttcccccagt gataagcaat
     4621 ttgccgtttg ctacttcatt actgtataga accattaaaa ctaagaaata aaagaataat
     4681 aggaatttgg cggaattttt cgggaataag tgaaacagaa gtattgaaaa caggggcttt
     4741 gcggtgcatt gcccctttgt tttatgtaga aaaaagtaaa aataatgaat tgcgaaaaaa
     4801 tggctaaaac taaccgcttg taccattaaa acaacgaaaa agtaaaaatg atttaagtag
     4861 ctttaaatgg cgtttaaatc atctcgtgtc caatgtacac aacctgcccg ataacctcaa
     4921 aatctaacgt ttcatcaaaa atcacatcaa tagggctgta tagctcttta ttatcgctga
     4981 ttaagcgaat accgcccata atgccttgca cacgtttaac ccaaagctgc tcgccttggc
     5041 ggaacacata aattttaccg tctttaggct gggtggtggc tcggtttacc aatagcatat
     5101 cgccatcact gatggttggg tacattgaat cacctgaagc ggtaataaat gcaagatagc
     5161 tcaaatagaa gccacgcaca tctagccatc gtttacttag cccaatatag tcatcagggg
     5221 catacacctc gctattgaaa gcaccaaagc ctgctgaagc ctgtacatta taaaacggca
     5281 cacgttccat ttcatctgca gtttgggctg ccactaatgt tctaggctct tcttttactg
     5341 ccgtttgagc aaagcctaac tcttgctgca cgcttattgg gagagagctg tagtggtatt
     5401 caaaagcaac cccccttttt ccctctattt gtcttttgag ccaattattt ttggtagctc
     5461 ttcttgttac atttgatggt tgttcaggta aatttcccat tccagctact tcagatgccg
     5521 taaaccattc ctttctaatt atagaattca taaaacccct tttg
//
LOCUS       DANLRK010000165         5564 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.165, whole genome shotgun sequence.
ACCESSION   DANLRK010000165 DANLRK010000000
VERSION     DANLRK010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5564
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.165"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            33..464
                     /locus_tag="PXA33_002180"
     CDS             33..464
                     /locus_tag="PXA33_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDL5121481.1"
                     /translation="MFLSGFLSATLLPGNSEIIFTTLASQHLITYGSLSSFSLFNLLL
                     VATLGNSLGSITTYAIGLLLPRPQNTTNRYSQWALAKSEKYGVLVLLFSWLPIVGDIF
                     CGIAGWLRFSLWQSILFIVIGKLLRYLFLLATLYSVLPHIL"
     gene            complement(518..1387)
                     /gene="htpX"
                     /locus_tag="PXA33_002181"
     CDS             complement(518..1387)
                     /gene="htpX"
                     /locus_tag="PXA33_002181"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HDL5121482.1"
                     /translation="MAKRVILFLLTNLAITFVLGIVLNLIFKVTGIQGQSTAGILVLS
                     LVFGFSGSLISLFMSKSMALRSVGAEVITQPRNEAEQWLFNTVQRQAQQANIPMPDIA
                     IYHSADVNAFATGATKSNSLVAVSTGLLNSMTKDEAEAVVAHEVAHIANGDMVTMTLL
                     QGVLNTFVIFLARIISTMAASSRDENGNSSTNTLVFWVVDIVLQMVFGVLATMIAMWF
                     SRQREFRADAGSAQLVGKEKMIAALQRLQRVHEPQEMEGSLAAFMINGSRGKELFMSH
                     PPLEKRIEALRNL"
     gene            complement(1475..2557)
                     /gene="dinB"
                     /locus_tag="PXA33_002182"
     CDS             complement(1475..2557)
                     /gene="dinB"
                     /locus_tag="PXA33_002182"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HDL5121483.1"
                     /translation="MSIQAKHKRKIIHIDMDCFYAAVEMRENPALVGKPVAVGGNANQ
                     RGVLTTCNYEARKFGLHSAMPTATALKKCPNLILLPVNMALYKSVSAQIHEIFHRYTD
                     VIEALSLDEAYLDVTECERYFGSATWIAQAIRNDIFNELHLTASAGVAPLKFLAKITS
                     DQNKPNGQCVITPDDVQNFVKNLPLAKIPGVGKVTNEKLKQLGFETCGDIQQAEKAVI
                     YRAFGKFGQRLWDFSHGIDERAVEPYRERKSLAVENTLLHDISTLEQAEKIVADLFDK
                     LVFRLQRNWGDTPLTEFKKLGIKLKFDDFSQTTLERTTDGFAYERFGELLQQIFTRRK
                     ERKVRLIGLSVHFPEKKVNKQLNLWE"
     gene            2680..3591
                     /locus_tag="PXA33_002183"
     CDS             2680..3591
                     /locus_tag="PXA33_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine exporter LysO family protein"
                     /protein_id="HDL5121484.1"
                     /translation="MLYGLAIVLIPLFLGYLITLKNHRHMAIVNRVVSLCLYLILLVM
                     GISLGQIDNILTELPQIGGTAFSLVVILLLSNIIGLVIYDKLQPMKRDKVNQDQLPSR
                     LHLLIDSFKLIGITLLGGIIGYFTKGVVDFPLHASTYVLEVMIFGVGIQLRNSGIPLR
                     EVFFNKRGIYTSIVMVASSLIGGVISAFTLDLSLAKGLTFASAFGWYSLSSVLVNDAW
                     GPIYGSIAFFNDISREILCLFLVPLFMRQFPSTAVGIGGATSLDCMLPIIQKSGGIQI
                     VPLAISFGFIVNLAAPLLLAVFIGLEG"
     gene            3697..5463
                     /gene="aspS"
                     /locus_tag="PXA33_002184"
     CDS             3697..5463
                     /gene="aspS"
                     /locus_tag="PXA33_002184"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HDL5121485.1"
                     /translation="MMRSHYCGALNRSHLGQTVTLSGWVHRVRNLGRFIFMQIRDREG
                     IVQVFFDEKDEALFKQASALRSEACVQIQGEVIARDESQINKEMATGEIEVLVKNLVV
                     YNNAEVLPLDFNQNNTEEQRLKYRYLDLRRPEMAEKLKTRAKITSFVRRFMDDNGFLD
                     IETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFR
                     DEDLRADRQPEFTQIDVETSFMTAEEVRALMEAMIHGLWKETLGVDLGKFPMMTWNEA
                     MTRFGSDKPDLRNPLEMVDVADILKSVDFKVFSGPANDPKGRVVALRVPNGAALTRKN
                     IDDYTQFVGIYGAKGLAWLKVNDMNTGLEGIQSPVAKFLNEEVLKALVKRLNLQKDDI
                     VFFGADNWHVATNAIGALRLKVGNDLGLTDTTAWKPLWVIDFPMFERDDEGNLSAMHH
                     PFTSPKELSPEELVQDPENAVANAYDMVINGYEVGGGSVRIFSQQMQQTVFSILGINE
                     EEQKEKFGFLLDALKFGTPPHAGLAFGLDRLTMLITGTDNIRDVIAFPKTTAAACLMT
                     EAPSYANPKALEELAIRTIPQE"
BASE COUNT         1564 a         1211 c         1137 g         1652 t
ORIGIN      
        1 tattcttttc cgaacaaaat caactcttta ctatgtttct gagtggattt ctaagcgcta
       61 ccctcttacc cggaaattct gaaattatct tcactacctt agcaagccaa catctcatca
      121 cttatggctc tttatcctca ttttcactgt tcaatttact cttggttgca accttaggta
      181 atagtttagg tagcataacc acttatgcga tagggctatt actccctcgc ccacagaata
      241 ctaccaatcg ttattcacaa tgggcattag ccaaaagtga aaaatatggc gtgttagtct
      301 tgctttttag ttggttaccg attgtaggcg atattttctg tggcattgcc ggctggctac
      361 ggtttagcct ttggcaaagc attctcttta ttgtgattgg gaaattgtta cgttatctgt
      421 ttttgctggc aactctctat tcggtgttac cgcatatttt atagaaaaca aaagcggctg
      481 ttttgcaaaa aattttacaa aaacaaccgc ttgtgcttta taagtttctt aacgcttcaa
      541 tacgtttttc taacggaggg tggctcataa acaactcttt accacggcta ccgttaatca
      601 taaaggcagc aagagagcct tccatttctt gcggttcgtg aacacgttgc aagcgttgta
      661 atgccgcaat cattttctct ttaccgacta attgagcaga gcctgcatct gcacgaaatt
      721 cacgttggcg agagaaccac attgcaatca ttgttgctaa cacaccaaac accatctgta
      781 acacaatatc tactacccag aacaccagcg tattggtgct tgaattgcca ttttcgtcac
      841 gtgaacttgc cgccattgtc gaaataatac gagctaagaa aatgacgaaa gtatttaaca
      901 cgccttgtaa taaagtcatt gtaaccatat cgccattcgc aatgtgcgcc acttcgtgtg
      961 ctactaccgc ttcagcttcg tctttagtca ttgaattcag caagccggtg ctgactgcaa
     1021 ctaaagaatt acttttcgtt gctcctgtag cgaatgcatt tacatcagcc gaatggtaaa
     1081 tcgcaatgtc cggcattggg atattcgctt gctgagcctg acgttgtaca gtgttgaata
     1141 accattgctc cgcttcgtta cgaggctgtg taatcacctc tgccccaacc gaacgcaatg
     1201 ccattgattt cgacataaat agcgaaatta aagacccact gaaaccgaag actaacgaaa
     1261 gcaccaaaat accggcagtg ctttgccctt gaatgccggt cactttaaaa ataaggttta
     1321 atacgatccc taaaacaaag gtaatcgcta agttagttaa taaaaataga atgacacgtt
     1381 ttgccataat gttttcaatc tccaaaaata tagatgcaat gattgcacga ataagataat
     1441 gactttagcc ttaatttcaa gtcattattt acaattattc ccacaaattt aactgtttat
     1501 tcactttttt ctcgggaaag tgaacactta gaccgattaa tcgcaccttt cgttcctttc
     1561 ttcgggtaaa aatttgctgt agcaactcgc caaatcgctc atacgcaaag ccatcggtgg
     1621 ttcgttcaag cgtggtttgg ctaaaatcat caaacttgag tttaatgcct aactttttaa
     1681 actcagttaa tggcgtatcg ccccagtttc gttgcaaccg aaaaaccagt ttgtcgaata
     1741 aatctgccac aatcttttcc gcttgctcaa gggttgaaat atcgtgcaac agcgtatttt
     1801 ccaccgccag cgattttctt tcacgataag gctctaccgc tcgttcatca ataccgtggc
     1861 taaaatccca aagccgttgc ccgaatttgc caaacgctcg gtaaataacc gctttttctg
     1921 cttgctgaat atcaccacag gtttcaaagc caagctgttt taatttttca ttggttactt
     1981 tccccacacc cggaattttt gcaagcggta gatttttcac aaaattttgc acatcatcag
     2041 gcgtaatcac acattgtccg ttaggtttgt tttgatcgga ggtaattttt gctaaaaatt
     2101 taagcggagc aactcctgcc gaggcagtta aatgcaattc attgaaaata tcattgcgga
     2161 ttgcttgagc aatccaagtg gcggagccaa aatagcgttc gcattcggtt acatctaaat
     2221 aggcttcatc aagcgacaag gcttcaatca catccgtata gcgatgaaaa atttcgtgaa
     2281 tttgagcgga aaccgattta tacaacgcca tattcaccgg caataaaatt aaattcgggc
     2341 atttcttcaa cgcagttgcc gttggcattg cactgtgtaa gccgaatttt cgagcctcgt
     2401 aattacaggt tgtcagcaca cctcgttgat tagcattgcc gcccaccgcc accggcttac
     2461 cgaccaacgc cgggttttcc cgcatttcga ccgctgcata aaaacaatcc atatcaatat
     2521 ggataatttt tcgcttatgt ttagcttgaa tcgacatttg caatattttc cccaaaaacg
     2581 accgcttgca acacaaataa ctgtatataa aaacagtttt attttatgcc cttattgtta
     2641 gaatttcaag tttaaaaata gcgataaaaa ggataaaaaa tgctctacgg tttagccatt
     2701 gtgctgattc cactttttct cggttactta ataaccctca aaaaccaccg ccatatggca
     2761 atagtaaaca gagtggtcag cctctgcctt tacttgattt tattagtgat ggggatttct
     2821 ctcggacaaa ttgataacat tctcaccgaa ctgccacaga ttggcggtac ggcgtttagt
     2881 ttagtggtga ttttactcct ttcaaacatt atcggcttgg tgatttacga caaattacaa
     2941 ccgatgaaaa gagacaaagt caatcaagat caacttccct cccgcctgca tttgttgatt
     3001 gattccttta aattaatcgg cattacctta ttaggcggga tcatcggcta tttcaccaaa
     3061 ggcgtggtgg atttccctct gcacgccagc acctatgtgt tagaagtgat gattttcggg
     3121 gtggggattc aactgcgaaa cagcggtatt cccctgcgtg aagtgttctt taacaagcgg
     3181 gggatttata cctcgattgt aatggtggca agttccctga ttggtggcgt gatttcggca
     3241 ttcaccttag atttatccct tgctaaaggc ttaacctttg cctctgcatt cggttggtat
     3301 tccctttcaa gcgtattggt aaacgatgct tggggcccga tttacggcag tattgccttt
     3361 tttaacgaca tctcgcgtga aattttatgc ctgtttttag taccactgtt tatgcgtcaa
     3421 tttccgtcca ccgcagtcgg cattggcgga gcgacttcat tagattgtat gctccctatt
     3481 attcaaaaat cgggcggtat tcaaattgtg ccgctggcaa tcagcttcgg ttttattgtt
     3541 aatttagccg ctccattgtt attagccgtt tttattgggc tggaaggata aatcaccgct
     3601 tgtttgcttt tatttatcca tcttcgctga attttccgct ataatcctta agttttcccc
     3661 gaaacttggg atatttattt tcaaaaggat aattttatga tgcgttctca ctactgcggt
     3721 gcattaaacc gctcacacct tggacaaaca gttaccttaa gcggttgggt tcatcgtgtt
     3781 cgtaaccttg gtcgttttat ttttatgcaa attcgtgatc gtgaaggcat tgtgcaggtg
     3841 tttttcgatg agaaagatga agcacttttc aagcaagcat cggccttacg ttctgaggcg
     3901 tgcgtgcaaa ttcaaggtga agtgattgcc cgtgatgaat cgcaaatcaa taaagagatg
     3961 gcaaccggtg aaattgaagt gttagtaaaa aatttagtgg tgtataacaa cgctgaagta
     4021 ttgccgttag acttcaatca aaacaatacc gaagaacagc gtttaaaata ccgctatttg
     4081 gatttacgcc gccctgaaat ggcagaaaag ctcaaaaccc gtgctaaaat caccagtttt
     4141 gtccgccgtt ttatggacga taacggtttc ttagacattg aaaccccgat gctgaccaaa
     4201 gctactcctg aaggtgcgag agattacctc gtgccaagcc gtgtgcataa aggcaaattc
     4261 tatgctctgc cacaatcacc tcagctattt aaacagttgc taatgatgtc gggcttcgac
     4321 cgttattacc aaatcgtgaa atgtttccgt gatgaagatt tgcgtgccga ccgccagccg
     4381 gaatttaccc aaatagacgt ggaaaccagc tttatgactg ccgaagaagt gcgtgcatta
     4441 atggaagcga tgatccacgg cttatggaaa gagaccttag gcgttgatct cggtaaattc
     4501 ccaatgatga cgtggaatga agcaatgacc cgtttcggtt cagacaaacc ggatttgcgt
     4561 aacccactgg aaatggttga tgtggcagat attttaaaat cggttgattt caaagtattt
     4621 agcggtccgg cgaatgatcc gaaagggcgt gtagtggcac ttcgtgtgcc aaacggtgca
     4681 gctctaaccc gtaaaaatat tgatgactac acccaatttg tcggcattta tggggcaaaa
     4741 ggcttggctt ggttaaaagt gaatgatatg aacacaggtt tagaaggcat tcaaagcccg
     4801 gttgcaaaat tcttaaatga agaagtgcta aaagccttag ttaaacgctt aaacttgcaa
     4861 aaagacgaca ttgtattctt cggtgcggat aactggcacg ttgcgactaa cgccatcggt
     4921 gcgttacgct taaaagtggg gaacgattta ggcttaaccg ataccacagc gtggaaaccg
     4981 ttatgggtga ttgatttccc aatgtttgaa cgtgatgatg aaggcaactt atcggcaatg
     5041 caccacccgt tcacttcacc gaaagaatta tcgccggaag aattagtgca agatccggaa
     5101 aatgcggtgg caaacgctta cgatatggta attaacggct acgaagtggg cggtggctcg
     5161 gtacgtattt tcagccagca gatgcaacaa accgtgttca gcattttagg cattaacgaa
     5221 gaagagcaaa aagagaaatt cggtttctta ttagatgcgt taaaattcgg tactccgcca
     5281 cacgcaggct tagccttcgg tttagaccgc ttaacgatgt tgattacagg aactgataac
     5341 attcgtgatg tcatcgcttt cccgaaaacc accgctgcag cgtgtttaat gacagaagca
     5401 ccaagctatg cgaatccgaa agcgttggaa gagttagcaa tccgtacaat tccacaagag
     5461 taatcggtag gggcgaaaca tttttcgccc tttttattta acaagcggtc gaaaaagtga
     5521 aaaactttgc aaaagtaata atcgcaaaat tattctctaa ataa
//
LOCUS       DANLRK010000166         5541 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.166, whole genome shotgun sequence.
ACCESSION   DANLRK010000166 DANLRK010000000
VERSION     DANLRK010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5541
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.166"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(<1..225)
                     /locus_tag="PXA33_002185"
     CDS             complement(<1..225)
                     /locus_tag="PXA33_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121486.1"
                     /translation="MSLLKKAQTFISIFNDVRTKNEAQRQSSNNLNVALKNAAEMRAE
                     MNGFSLKPQKRSTYFADHYGCGIHAVETYDN"
     gene            complement(295..846)
                     /locus_tag="PXA33_002186"
     CDS             complement(295..846)
                     /locus_tag="PXA33_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121487.1"
                     /translation="MCNQKTLSLYGLVKELGIEKTQLFLEQAFSCSRNKDVESFLLNK
                     AIRFETALAAATHLIINEQGDILAYFTLSFKEIEVEVSKSQLKKLTAGLSNDSHLRVL
                     LIGQIGKNEKVIDNPAKLPDILNHIYAQLYKAQRVLGGRVVILECENTPKLLELYKQN
                     KFQILNTIGSENGLKTLFFIPDF"
     gene            complement(807..983)
                     /locus_tag="PXA33_002187"
     CDS             complement(807..983)
                     /locus_tag="PXA33_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121488.1"
                     /translation="MATSSFDKRFVVKDPKAVKVLIEALQSPIKVVLPKKDLEKEEKE
                     ALCAIKKRLASMGL"
     gene            complement(1183..1437)
                     /locus_tag="PXA33_002188"
     CDS             complement(1183..1437)
                     /locus_tag="PXA33_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121489.1"
                     /translation="MICHADPEYVKYNYAGLWEKGSDGTFFCNYQQFMPEVEAKESLR
                     KQEPELDDRYNEEEYSEEEYPDENDASDENDNYDETYNVF"
     gene            1676..1930
                     /locus_tag="PXA33_002189"
     CDS             1676..1930
                     /locus_tag="PXA33_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121490.1"
                     /translation="MTNQQLLAIAQEYRERYQDNHPCMLSSGYVRIYQGEAYGWCAEK
                     GEANTECPEALLVSLTGDIFEAVGGDKLKGAKKWQPYKKE"
     gene            1934..2080
                     /locus_tag="PXA33_002190"
     CDS             1934..2080
                     /locus_tag="PXA33_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121491.1"
                     /translation="MDTTQKRVALFIIGLSVVMWLRKHNCCTVIFVLLLAALAFSYTM
                     EHLY"
     gene            2096..2302
                     /locus_tag="PXA33_002191"
     CDS             2096..2302
                     /locus_tag="PXA33_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121492.1"
                     /translation="MVKNVVHEEILHNRAELQQAVEKEEFCAKCDNSVVFHLRDNYHE
                     FTLGLDTILQCLHFAEEKGAIRIP"
     gene            2299..3075
                     /locus_tag="PXA33_002192"
     CDS             2299..3075
                     /locus_tag="PXA33_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121493.1"
                     /translation="MNIEYSLNHCQGDGVAFYGRLGTDDLIQLFNHLNPQKRKQKMFA
                     NLLTHIESWETHDDIGFEIIRNRFGYRYSHWNTMELYAKTADQLVFFNDSDARRNWYF
                     PTSKVYKYKGLWDEFIQDLEQYIKGTSREMESVGYQIIEASPYQTETVFKFNTENYLV
                     ELKRKPSEFYSELVDWAFGDMCDFEATIEQICQGQLCYADFEATVTDKKSEIQLGLAR
                     LSGISYSPNDRSFSGYRQELIGEAIASARHNAARFAYLHA"
     gene            3190..3602
                     /locus_tag="PXA33_002193"
                     /pseudo
     CDS             3190..3602
                     /locus_tag="PXA33_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484619.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemophilus-specific protein"
     gene            3616..3771
                     /locus_tag="PXA33_002194"
     CDS             3616..3771
                     /locus_tag="PXA33_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121494.1"
                     /translation="METRIVKVIYDDGDYFFTRINADEQAIRNYYAIGSSIGFDDIRK
                     IIAVEIL"
     gene            3851..4825
                     /locus_tag="PXA33_002195"
     CDS             3851..4825
                     /locus_tag="PXA33_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1738 domain-containing protein"
                     /protein_id="HDL5121495.1"
                     /translation="MNTQPKFDLYQHITDRIVVALEQGTAPWLKPWSHPECNMALPRN
                     AVSNRLYSGINTLLLWIATAEHGYKQCKWITAKKANELGGYIRKGEKATVVMNYRPDE
                     REKLDEEGNIVYDEEGNPEMERHFFINKHNLFNLDQCENLPVELYDPVSEPKQPKSEP
                     NQYDIFAEIRQIIKGMDLQVEMKPSNRAFYQPGEDKVVMPEMKQFANAQAFHSVLLHE
                     MTHATGANNRLNREGITSGKAKFGNKVYAFEELIAEMGGAFLCAYLGFDTVPQNAAYI
                     ESWIRVLKEDKRAIVRASGKAREACQYMLDALNVAQLADKYDVFEEAA"
     gene            4912..5109
                     /locus_tag="PXA33_002196"
     CDS             4912..5109
                     /locus_tag="PXA33_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121496.1"
                     /translation="MTKSTEIPRGHDNWVITGRDNDGKWHKERFLYYSRREAIQQFRR
                     KHNCVGKHSVIESITPQFGWY"
     gene            5186..5428
                     /locus_tag="PXA33_002197"
     CDS             5186..5428
                     /locus_tag="PXA33_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121497.1"
                     /translation="MFNLNLFKRPIELSTLESWSKFCDDVGKVAFIAIPPMIYSNYDL
                     CIKIGNISALLAITYGFLLSGRTIRQFIEKEKSRWD"
BASE COUNT         1686 a          955 c         1138 g         1762 t
ORIGIN      
        1 attatcatag gtttctactg catgaatacc acaaccataa tggtcagcaa agtaagtaga
       61 acgcttttga ggcttaaggg agaacccatt catctcagct ctcatttcag cagcattttt
      121 caaggcaaca tttaaattgt ttgaagactg gcgttgagct tcatttttag ttcgcacgtc
      181 attaaaaata gaaataaacg tttgtgcttt tttaagtaga gacattgttc tttctcccat
      241 atatgacttc ctgacaaagc tggcgacttt cttctaatga aatattagct gtagttaaaa
      301 atcaggaata aagaaaagtg tttttagacc attctcactt ccaatggtat tgagaatttg
      361 aaatttattt tgtttatata gttctaataa tttaggcgta ttttcacatt ctaatataac
      421 gactcgccca cctaaaactc gttgtgcttt ataaagttgt gcatagatat gatttagaat
      481 atctggcaat ttggctggat tgtcaattac tttttcattt ttgccgattt gaccgataag
      541 caatactctt aaatgtgaat cattacttaa accagcagtt agctttttta attggctttt
      601 gcttacttcc acctcaattt ctttaaaaga gagggtaaag taagccaaaa tatcgccctg
      661 ttcattaatg attaaatgtg ttgctgctgc taatgcggtt tcaaatctga ttgctttatt
      721 aagtagaaaa ctttcaacat ctttatttct actgcaagaa aatgcttgct ccaaaaagag
      781 ttgtgttttt tcaataccta gttcttttac aagcccatag aggctaagcg ttttttgatt
      841 gcacatagtg cttctttctc ttctttttct aagtcttttt taggtaaaac tactttgatt
      901 gggctttgta atgcctcaat taataccttg acagcttttg gatctttgac tacaaagcgt
      961 ttatcaaatg atgatgttgc cattatagaa ctccttaaat atttaaatta ccatggttac
     1021 tcatggctgt aataatactg tatttaggcc ttgtgggtgg ttttcgatta catagcctta
     1081 tgcttgttag catagaaata ctttcaatta ccacaatata tagactatat ttggaaatgc
     1141 aagccttttc aatcacggat tcctccggtt tccatataca tactagaaaa cattataagt
     1201 ttcatcgtag ttatcgtttt catcgctggc atcattttca tcaggatatt cttcttcgct
     1261 atattcctct tcgttatatc tatcatcaag ttctggttct tgtttcctta gtgattcttt
     1321 agcttcaact tcaggcataa attgctggta attgcagaaa aaagtgccat cgctgccttt
     1381 ttcccaaagt ccggcgtagt tgtattttac atattcagga tcagcgtggc atatcatccc
     1441 taattgaggg gtggcataag tgagcgacat cgaaatgcta ctaaggataa gcgtgaggag
     1501 ttttggtttc acagcaaaat agggttaggt aaatttactt cagtgtatgg aaaaacatag
     1561 gaatgttgag cagaaactaa aaatgatttg aatatagttt ttggtttcta tttataaaaa
     1621 tgttgccaga atactaagaa cgttttcagg tttattatgt tattggagac tttatatgac
     1681 taatcagcaa ttactagcta ttgctcaaga gtatcgtgag cgttatcaag ataaccatcc
     1741 ctgtatgttg agtagcggct atgttcgtat ttaccaaggt gaggcttatg gttggtgtgc
     1801 tgaaaaagga gaggcaaata cagaatgccc tgaggcctta ttggtgagtt taaccggcga
     1861 tatttttgaa gcagtcggcg gagataagct gaaaggtgcc aaaaaatggc agccttacaa
     1921 gaaagaatga cagatggata cgacacaaaa acgtgtggct ttattcatca ttggattatc
     1981 tgttgtgatg tggctaagaa agcataactg ctgtacagtg atatttgtat tgttgttggc
     2041 agcgttagca tttagctaca cgatggaaca tttatattag ctggggaact gaattatggt
     2101 aaagaatgtg gttcatgaag agatattaca taaccgtgca gaattacagc aagcggttga
     2161 aaaagaagaa ttttgtgcaa aatgtgataa ctcagtagta ttccatttac gtgataatta
     2221 tcacgaattt acgcttgggt tagatactat attgcagtgt ctgcattttg ctgaagagaa
     2281 gggagctatc cggatacctt gaatattgag tattcactca atcattgcca aggagatgga
     2341 gtcgcatttt atggtcgact tggtacagat gatctaatcc agcttttcaa tcatctcaat
     2401 ccacagaaac gtaaacagaa aatgtttgct aatttgctaa cgcacattga atcttgggaa
     2461 acccacgatg atattggttt tgaaattata cgaaatcgct ttgggtatcg ttactcgcat
     2521 tggaatacga tggagcttta tgctaaaact gcagatcagt tagtgttttt caatgatagt
     2581 gatgctcgaa gaaactggta ttttccgact tcaaaagtgt ataaatacaa aggcttgtgg
     2641 gatgagttta ttcaggattt agaacagtat attaagggta cttcccgtga aatggaatcg
     2701 gtcggttatc aaattattga agcctcgccg tatcaaacgg aaaccgtttt caaatttaat
     2761 acggaaaact atctagtaga actgaaacgt aaaccttctg agttttattc agagcttgtt
     2821 gattgggcat ttggtgatat gtgtgatttt gaagcaacta tagagcagat ttgtcaaggg
     2881 caactctgtt atgctgattt tgaagcaact gttaccgata agaaaagtga aattcagtta
     2941 ggtcttgctc gcttgagtgg aataagttac tcgccgaatg accgttcgtt tagtggttat
     3001 cgacaagagc ttattggtga ggcgattgcc tctgctcgcc ataatgcagc acgttttgcc
     3061 tacttgcacg cctaatttgc agatttgtac ttcaatttga ccgcttgtag tggttaattt
     3121 tcaccccgtg gggaaatatt ttccctattg ggcaagatgt ttctccactt tttaatttgg
     3181 agaaatgtga tgaaaaatga acacaattta gagcaactta aatatatttg ggataaccgc
     3241 aaccttgaaa ataaagccta tcaatacctg tttcacattt gcattgagcc gatgaaaaaa
     3301 ggagattgga atcgttatac gatttggctt aattttcacg gcaacttaat caacgtgtca
     3361 gacgttgtat atcgagttgt ggaatctcaa aagctcaagg atgacatcaa aatgtggtat
     3421 gacaataata ccattgtcgt ccgataaaag ttgaaacaac aggcttcatc tcatatttac
     3481 tgcaccagtt ctatcagggg atagcctata ttttactgga acagggaatg attactgaaa
     3541 ctgaagcagc cgagaaaagt tataacttca gagctgcaaa ttgttttcaa cgtttgcttt
     3601 aatggaggtg agataatgga aactcgcatt gttaaagtta tttacgatga tggtgattat
     3661 ttctttaccc gaatcaatgc tgatgaacag gctatccgta attactacgc cataggcagt
     3721 tcaataggtt ttgatgatat aagaaaaatt atcgctgttg aaattttata aaccacccat
     3781 aggggaaact atttccccat agggtgatag cgtttcccca tttttctacc cgaactttgg
     3841 agaaacactt atgaacactc aacctaaatt tgatttatac caacacatca ctgaccgaat
     3901 tgttgtagca ctggaacaag gtacagcccc gtggttaaaa ccgtggagcc atccggaatg
     3961 caatatggca ttacctcgca atgcggtatc taaccgcttg tattccggta tcaatacact
     4021 cttactctgg attgctactg ctgaacatgg ttacaaacag tgcaagtgga ttacagcaaa
     4081 aaaagccaat gaactcggcg gatatatccg taaaggggaa aaagctactg tggtgatgaa
     4141 ctaccgtcct gatgagagag agaaactcga tgaggaaggg aatattgtgt atgacgagga
     4201 aggaaatccg gaaatggaac gacacttttt cattaataag cacaatctgt tcaatctgga
     4261 ccagtgtgaa aatttaccgg tggaactgta cgatcctgta tcagaaccga aacaacctaa
     4321 atctgagcca aaccaatacg atatttttgc tgagattcgg cagattatca aaggaatgga
     4381 tttgcaggtg gaaatgaagc ctagcaatcg ggcattttat caaccaggtg aagataaggt
     4441 cgtaatgccg gagatgaagc agtttgcaaa tgctcaagcg tttcatagtg tattgttaca
     4501 cgaaatgact cacgcaacgg gggcaaataa tcggcttaat cgggaaggta ttacttccgg
     4561 aaaggctaaa tttggaaaca aagtctatgc ttttgaggag ttgattgctg aaatgggcgg
     4621 tgcattttta tgtgcctacc ttggttttga tactgtgcct caaaatgcgg cgtatattga
     4681 aagttggatt cgagtattga aagaagataa acgggcgatt gtccgtgctt ccggaaaagc
     4741 tcgtgaagcc tgccaataca tgttagacgc attaaacgtc gcacagttgg ctgacaaata
     4801 cgatgtattt gaagaagctg cttaacaaat ttgccgaaag gcatatctca aggggagacc
     4861 tatccctttg ggcatagtgt ttccctattt tttattaaat ggagaaaagc tatgacaaaa
     4921 tcaactgaaa tacctcgtgg acatgataac tgggttatta caggcagaga caatgatggg
     4981 aaatggcata aagaacgttt tctttattat tctcgccgtg aagccatcca acagtttcgt
     5041 cgaaaacaca actgcgtagg taaacatagt gttattgaat caataacacc tcaatttggc
     5101 tggtattaac cggctttccc gtggggagat actctccctt cggagcattg tgtctccctt
     5161 tttattcact taactagagg acacaatgtt caatctaaac ttatttaaac gccctattga
     5221 gctttcaacg cttgaatctt ggtctaaatt ctgtgatgat gtcggtaaag ttgcatttat
     5281 tgcgatacct ccgatgattt atagtaatta tgatctatgt atcaaaatag gaaacataag
     5341 tgctttattg gcaattacct atggattctt attatcaggc aggactatcc gtcaatttat
     5401 tgagaaggag aaatcaagat gggattaaca atcggcttat ggatcattgc ttttataggc
     5461 tttgcactgg ttttttgggc aaatagctta ctcaaagagc aagatgaacg ccttaaacag
     5521 ctaaaaaaat agctcaactt t
//
LOCUS       DANLRK010000167         5507 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.167, whole genome shotgun sequence.
ACCESSION   DANLRK010000167 DANLRK010000000
VERSION     DANLRK010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5507
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.167"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            161..1294
                     /gene="carA"
                     /locus_tag="PXA33_002198"
     CDS             161..1294
                     /gene="carA"
                     /locus_tag="PXA33_002198"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HDL5121498.1"
                     /translation="MFKPAILVLTDGSVFSGKSIGADGHTIGEVVFNTAMTGYQEILT
                     DPSYTKQIVTLTYPHIGNTGINLEDKESPNVCASGLIIRDLPLLHSNFRASSSLSDYL
                     IQQNIVAIADIDTRRLTRILREKGAQAGCILVGDATEENIANALAFANSFGSMAGKDL
                     AQSVTCNELYKWTDGEWQLGKGYVPQQQTEFHVVAYDYGVKHNILRMLAERGCRLTVV
                     PAKTSAEAVLALNPDGIFLSNGPGDPEPCDYAITAIRKLLETQKPIFGICLGHQLLGL
                     AAGGKTKKMVFGHHGANHPVQDLDSQKVLITSQNHGFEVDESSLPSNVRVTHRSLFDG
                     TVQGIELTDRPAFSFQGHPEASPGPNDVAYLFDRFVSEMKKVK"
     gene            1397..4603
                     /gene="carB"
                     /locus_tag="PXA33_002199"
     CDS             1397..4603
                     /gene="carB"
                     /locus_tag="PXA33_002199"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HDL5121499.1"
                     /translation="MPKRTDINTILIIGAGPIVIGQACEFDYSGAQACKALREEGYKV
                     VLVNSNPATIMTDPNMADVTYIEPIEWKTVEKIIEKERPDAILPTMGGQTALNCALDL
                     SKNGVLKKYNVELIGATEDAIDKAEDRGRFKEAMTKIGLNTPKSFVCHSFEEALAAQE
                     QVGFPTLIRPSFTMGGSGGGIAYNRDEFIAICERGFEASPTHELLIEQSVLGWKEYEM
                     EVVRDRADNCIIVCSIENFDPMGVHTGDSITVAPAQTLTDKEYQIMRNASLAVLREIG
                     VDTGGSNVQFAINPANGEMIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLNE
                     LRNDITGGLIPASFEPTLDYVVTKIPRFAFEKFANADDRLTTQMKSVGEVMAMGRTFQ
                     ESLQKALRGLETGICGFNLLSEEPEKIRRELANPGPNRILYVADAFGAGFSLEEVHHY
                     SKIDPWFLVQIQDLVLEELALEKKQFSDLDANEIRRLKRKGFSDKRIAQLTKVSEKMV
                     RDYRHTYAILPVYKRVDTCAAEFASDTAYLYSTYEEECEAKPSDRKKVMILGGGPNRI
                     GQGIEFDYCCVHASLALREAGYETIMVNCNPETVSTDFDTSDRLYFEPLTLEDVLEIV
                     RVENPWGVIVHYGGQTPLKLANALSDAGVNIIGTSADSIDAAEDRERFQQILNTLGLK
                     QPANRTARNALEAVALANEVGYPLVVRPSYVLGGRAMQIVYNDEELNRYMREAVSVSN
                     DSPILLDHFLNNAIEVDVDCICDGENVLIGGIMQHVEQAGIHSGDSACSLPAYSLSGH
                     VLDEIRKQTNAMAFALGVKGLMNVQFAVQNEVVYVLEVNPRASRTVPFVSKATGRPLA
                     KIAARVMAGETLADLNALEEIIPSKFFVKEAVFPFIKFPGVDTILGPEMRSTGEVMGI
                     GNTFAEAFFKAQLGAGERIPKVGKVFMSVIEEHKPALIYIAKRFQEQGYGLCATFGTA
                     NFLRQHGIAVQTVNKVREGRPHIVDAIKNGEIALVINTVSGEPEVIADSHSIRRTALQ
                     QRVPIYTTLAGADAISYGVKHINEFDVYSVQELEQKAN"
     gene            complement(4678..>5507)
                     /locus_tag="PXA33_002200"
     CDS             complement(4678..>5507)
                     /locus_tag="PXA33_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HDL5121500.1"
                     /translation="TSGHFFAKSDRLYNVEDSLQENGSARESLAYSARIEIGLKTFLD
                     NGGFKAFTDNFQNLNGIKQLPGLAVQRLMEQGYGFGGEGGWKTAALVREVKVMGYGLP
                     NGSSFMEDYTYDLDEQNQVVLGAHMLEVCPSIAKEKSTLAIRPLGIGGKADPVRLIFS
                     SKAGKAVNATVVDMGDRFRMVVADLDAIASPNPMPNLPVGHAFWKLQPNFYVGTQAWI
                     LAGGAHHSVFSLDIDADMLRMFAEYFGIEFIHINQNTELPQLKNELRWNDLAYKFTK"
BASE COUNT         1584 a         1255 c         1233 g         1435 t
ORIGIN      
        1 tgcatatatt ttcacttccg agtagaaaat gaaataaaaa aagaagaaag aaataggcaa
       61 aataactaat ttttacttga atgcaaacgt ttgcttgatt aaacttagtc gcctacttct
      121 aaggctacac agataaaatt tgatgattag gagtaaaaca atgttcaaac cagcaattct
      181 ggttctcact gacggttctg tcttttccgg caaatccatt ggtgctgacg gtcacactat
      241 cggtgaagtg gtatttaaca ctgcgatgac cggttatcaa gaaatcctta ccgatccttc
      301 ttatactaaa caaatagtca ccctgactta cccccacatt ggcaataccg gcattaattt
      361 agaagataaa gaatccccca atgtttgtgc ctccggctta attattcgtg atcttcctct
      421 tctccatagc aacttccgtg cgtcctcctc actttcagat tatctcattc aacaaaacat
      481 tgtcgctatt gccgatattg atacccgccg tttaacccga attttacgcg aaaaaggggc
      541 acaagccggt tgtattttag taggcgatgc cacagaagag aatattgcta acgctttggc
      601 atttgccaac agtttcggtt ctatggctgg gaaagatttg gctcaaagtg tcacctgcaa
      661 tgaattatat aaatggacag atggtgaatg gcaattaggt aaaggttatg taccgcaaca
      721 acaaaccgag ttccacgtag ttgcctatga ttacggtgta aaacacaaca ttttgcgaat
      781 gcttgccgaa cgaggctgtc gcttaaccgt tgtgccggca aaaacctctg ctgaagctgt
      841 gcttgcgtta aatccggacg gtattttcct ttctaacggc ccgggggacc cggagccgtg
      901 cgattatgcc attaccgcaa tccgaaaatt attggaaacg caaaaaccca ttttcggcat
      961 ttgcttaggg catcaacttt taggtttagc ggcaggcggt aaaaccaaaa aaatggtgtt
     1021 tggtcatcac ggagctaatc acccggtaca agatttagac agccaaaaag tgctcatcac
     1081 cagccaaaac cacggttttg aagtagatga atccagctta ccatctaatg tgcgagtgac
     1141 tcaccgctca ctgtttgacg gcacggtgca ggggattgaa ctcactgata gaccggcgtt
     1201 ttcgttccaa ggtcacccgg aagccagccc ggggccgaat gatgttgcct atttatttga
     1261 ccgatttgtc agtgagatga aaaaagtgaa gtaaggacgt acctatgtgt gtccatcaag
     1321 cggtcgaaat ttgcaaaaaa cttaccaaaa atgaccgctt gtagcctgaa ataaacgaga
     1381 taaaagagag cccaaaatgc caaaaagaac agacataaac actatcctaa ttatcggtgc
     1441 cggtccgatt gtgatcggtc aagcgtgtga atttgactac tccggagccc aagcgtgcaa
     1501 agcgttgcgt gaagaagggt ataaagtggt gttggtgaac tccaaccctg caaccattat
     1561 gaccgacccg aatatggcag atgtgactta cattgagccg attgaatgga aaacggttga
     1621 aaaaatcatt gaaaaagaac gccctgatgc cattttaccg actatgggcg ggcagacagc
     1681 gttgaactgt gccttagatc tttcgaaaaa cggtgtgctg aaaaaatata atgtagagct
     1741 gatcggagct accgaagacg cgattgataa agccgaagat cgtggtcgtt ttaaagaagc
     1801 aatgactaaa atcggcttga atacgccaaa atcattcgtt tgccacagtt ttgaagaggc
     1861 cttagcagca caagagcagg ttggctttcc aaccctgatt cgcccctctt tcacgatggg
     1921 aggctccggc ggtggtattg cttataatcg agatgaattt atcgccatct gcgaacgtgg
     1981 ttttgaagcc tcgcctactc acgaattact gattgaacaa tccgtactag gctggaaaga
     2041 gtatgaaatg gaagttgtgc gtgaccgtgc cgataactgc attatcgtct gctcgattga
     2101 aaacttcgac ccgatgggtg tacacaccgg cgactccatt accgttgctc cggcacaaac
     2161 gctaaccgat aaagagtatc aaattatgcg taacgcttcg ctggcggtat tgcgtgagat
     2221 cggcgtggac accggcggtt caaatgtgca atttgcgatt aaccctgcaa acggcgagat
     2281 gatcgtcatt gaaatgaacc ctcgtgtgag ccgttcttct gcactcgcat ctaaagccac
     2341 cggcttcccg attgccaaag ttgcggcgaa attggctgta ggctacacac tcaacgaatt
     2401 gcgtaacgac atcaccggag ggctgattcc ggcttcattc gagccgactt tagattatgt
     2461 tgtgaccaaa attccacgct tcgcctttga aaaatttgcc aatgcagacg accgattaac
     2521 cactcaaatg aaatcggtag gcgaagtaat ggcaatggga agaaccttcc aagagagttt
     2581 acaaaaagcc ttgcgaggct tggaaaccgg catttgcggc tttaacttgc tttccgaaga
     2641 gcctgaaaaa atccgccgtg aacttgccaa ccccggacca aaccgtattt tgtatgttgc
     2701 cgatgccttt ggagcaggct tttcattgga agaagttcac cactattcta aaatcgaccc
     2761 gtggttcttg gtgcaaattc aagacttagt acttgaagaa ttagcgttag agaaaaaaca
     2821 gtttagcgat ctagatgcta acgaaatccg ccgcttaaaa cgcaaaggct tctcagacaa
     2881 acgtatcgca cagcttacca aagtcagcga aaaaatggtg cgggattacc gccatactta
     2941 cgctattcta ccggtgtata aacgggtaga tacctgtgca gccgagtttg cctcagatac
     3001 ggcttacctc tattctactt acgaggaaga gtgtgaagcc aaaccgtctg atcgcaaaaa
     3061 agtgatgatt ttaggcggcg gaccaaaccg tatcgggcag gggattgaat ttgactactg
     3121 ctgcgttcac gcctcgcttg ctctgcgtga ggcaggttat gaaaccatta tggtgaactg
     3181 caaccctgaa accgtttcca ccgatttcga cacctccgac cgcctctatt tcgagccgct
     3241 aacattagaa gatgtactgg aaattgtgcg tgtagaaaac ccgtggggcg tgattgtgca
     3301 ttacggcggt caaacaccac tgaaattagc gaatgcctta tctgatgccg gtgtaaacat
     3361 tatcgggact tccgccgaca gcattgatgc cgccgaagac cgtgaacgtt tccaacaaat
     3421 tctaaacact ctcggtttaa aacagccggc aaatcgcacc gcccgtaatg cgttggaagc
     3481 ggtggcattg gcaaatgaag taggctatcc gctggtggtt cgcccatctt atgtgctagg
     3541 tggtcgggca atgcaaattg tgtataacga tgaagagcta aaccgctata tgcgtgaagc
     3601 ggtatcggtt tccaacgaca gcccgatttt actcgaccac ttcctcaata atgcgattga
     3661 agttgatgtg gattgcattt gtgacggcga aaatgtactc attggcggca ttatgcaaca
     3721 cgttgagcaa gccggtattc acagtggcga tagtgcctgc tcactacctg cctattcgtt
     3781 aagcggtcat gttttggacg aaattcgcaa acaaaccaac gcaatggcgt ttgctctcgg
     3841 tgtgaaaggc ttgatgaacg tacaatttgc agtacaaaat gaggtcgttt atgtgttgga
     3901 agttaaccct cgagccagcc gtactgtacc gtttgtcagc aaagctaccg gtcgcccact
     3961 ggcaaaaatc gctgctcgcg tgatggctgg tgaaaccctt gccgacttaa atgcattaga
     4021 ggaaattatc ccaagcaaat tcttcgttaa agaagcggta ttcccattca tcaaattccc
     4081 gggtgtggat accattctcg ggcctgaaat gcgttctacc ggcgaagtga tgggcatagg
     4141 taataccttt gctgaagcgt tcttcaaagc acaactgggt gccggtgaac gaattccaaa
     4201 agtcggcaaa gtgtttatgt cggttattga agagcataaa cctgctttaa tttacattgc
     4261 caaacgtttc caagaacaag gctacggttt atgtgccacc ttcggcactg caaacttcct
     4321 acggcaacac ggaattgcag tccaaacggt aaacaaagtt cgtgaagggc gaccgcatat
     4381 tgtcgatgcg atcaaaaacg gcgaaattgc gttggtaatc aacaccgtca gcggcgagcc
     4441 tgaagtgatt gccgacagcc actctatccg ccgtaccgcc ttgcaacaac gtgtgcctat
     4501 ttacaccacc cttgcaggtg ctgatgctat ctcctacggc gtaaaacata tcaatgagtt
     4561 tgatgtgtat tcagtgcagg aattagaaca aaaggctaac taaaataaag ctaaaatgac
     4621 aagcggtcag attttgcaaa ttttttacaa attctgaccg cttgtgtttg tagaggatta
     4681 tttagtaaat ttatatgcta aatcattcca gcgtaattca tttttgagtt gtggtagctc
     4741 ggtattttgg ttgatatgga taaactcaat accaaaatat tcagcgaaca ttcgtagcat
     4801 atcggcatca atatctaaac taaatacact atgatgagca ccgccggcta gaatccatgc
     4861 ttgagtacct acataaaaat ttggttgaag cttccaaaaa gcatgcccaa ctggtaaatt
     4921 tggcattgga tttggtgagg cgatagcgtc taaatccgca acaaccattc taaaacggtc
     4981 tcccatatct acaacggttg cattcactgc ttttcccgcc ttactactga aaattaagcg
     5041 aacaggatct gccttaccgc caatacctag cggtcgaatg gctaatgttg atttctcttt
     5101 ggcaatagac ggacaaactt caagcatatg agcccccaat accacttggt tttgttcatc
     5161 taaatcatag gtgtagtctt ccataaagga agaaccgttt ggtaagccat aacccatcac
     5221 tttcacttca cgcacaagag ctgcggtttt ccaacctcct tcaccaccaa atccgtagcc
     5281 ttgttccatt aaacgctgaa cagccaagcc cggtaattgt ttaatcccat ttagattttg
     5341 gaaattatca gtaaaagctt taaatccacc attatctaag aatgttttta agccgatttc
     5401 aatacgagca gaatatgcga gagattctct tgctgaacca ttttcttgca agctatcctc
     5461 aacattatac aagcggtcag attttgcaaa aaaatgaccg cttgttg
//
LOCUS       DANLRK010000168         5496 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.168, whole genome shotgun sequence.
ACCESSION   DANLRK010000168 DANLRK010000000
VERSION     DANLRK010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5496
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.168"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            34..2100
                     /gene="glyS"
                     /locus_tag="PXA33_002201"
     CDS             34..2100
                     /gene="glyS"
                     /locus_tag="PXA33_002201"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005693270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HDL5121501.1"
                     /translation="MTTQNFLAEIGTEELPPKALKKLATAFAENVENELNQAGLSFEK
                     VEWFAAPRRLAVKVLSLAESQPSKEVEKRGPAVSAAFDAEGKPTKAAEGWAKGCGITV
                     EQAERIATDKGEWLVHRAVIEGQPTKNLLVDIIAKSLANLPIPKTMRWGDKTEQFVRP
                     VHTVTLLFGAELIEGEILGVKSGRTIRGHRFLGEREFQIENADQYPQLLKDRGSVVAD
                     FNERKALILAKSQEKATALGGVADIEEDLLDEVTSLVEYPNVLTAKFEERFLAVPAEA
                     LVYTMKGDQKYFPIYSKDGKLLPHFIFVSNINPEDPSKIIEGNEKVVRPRLTDAEFFF
                     KTDLKQKLEDQLPRLETVLFQQQLGTLRDKTARIEALAGEIAKQIGADETKAKRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEDEEVAVALNEQYMPRFAGDNLPNSLVAC
                     SVALADKFDTLTGIFGIGQAPKGSADPFALRRAALGSLRIIVEKNLPLDLTDLVAKSA
                     QLFGDKLTNKDVVEDVVDFMLGRFRAWYESEGIAVDVIQAVLARRPTKPADFDARVRA
                     VSHFRTLDSAEALAAANKRVSNILAKADIAIGDIDVSACVEPAEKALAEAVIALKAEV
                     QPLIAQGDYTAVLDKLANLRQPVDAFFDGVMVNAEDQKLRQNRLAILSTLQGLFLQVA
                     DISLLQ"
     gene            complement(2205..3506)
                     /locus_tag="PXA33_002202"
     CDS             complement(2205..3506)
                     /locus_tag="PXA33_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HDL5121502.1"
                     /translation="MNIHCEKLVQSILHAVNHEVVPALGCTEPISLALASAIARQYLG
                     HLPERIEAKVSPNLMKNGMGVAVPGTATVGLPMAAAIGAIGGEPDGGLEVLKNITPEQ
                     VKQAKAMLNTDKVSVSIFETEHILYSEATLFHQANRVCVRIAEHHTNVIYIEKNGQTI
                     LSKPCAVNNDNTTEIFASLSAKDIFNFSMQIELEKIRFIEQAAQLNSALSQEGLRADY
                     GLHIGRTLQKQIGLGLISDDLLNRIVIETTSASDARMGGASLPAMSNSGSGNQGITAT
                     MPIVVVARHLNVTDEQRIRALFLSHLMAIYIHSKLPKLSALCAVTTAAMGSCAGIAWL
                     LTGKFENISMAISSMIGDISGIICDGASNSCAMKVSTSVTSSYKSILMSMDNSQVTGN
                     EGIVEHDIDRSINNLCSIASRSMQYTDRQVIEIMVNKPKLK"
     gene            complement(3520..4797)
                     /locus_tag="PXA33_002203"
     CDS             complement(3520..4797)
                     /locus_tag="PXA33_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121503.1"
                     /translation="MDTQKLSPAEFKQATKFDSTDFGWIIMSIGMAIGAGIVFLPVQV
                     GLMGMWVFLLSSIIGYPAMYLFQRLFINTLAESPECKDYPTVISGYLGKNWGIFLGVL
                     YFIMLVIWMFVYSTAITNDSASYLLTFGITNTLLSDNPFYGLVLICILVALSSRGEKL
                     LFKISTFMVLTKLFVVAALGLAMIGMWQLHNIGAMPPFGKLIKDAIITLPFTLTSILF
                     LQTLSPMVISYRAKEQSREVARYKALRAMNIAFAILFITVFFYAVSFTLAMGREEALK
                     AYEQNVSALAIAAQFFPGSWATYVGVALNIFAVMTAFFGVYLGFREATQGIVMNLLLR
                     KFPAEKINPNYVQKGIMLFSILLAWSAIVLNAPVLSFTSICSPIFGLVGCLIPAYLVY
                     KVPALFKYKGLALNIIIITGILLCISPFLTFLE"
     gene            complement(4826..5494)
                     /locus_tag="PXA33_002204"
     CDS             complement(4826..5494)
                     /locus_tag="PXA33_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HDL5121504.1"
                     /translation="MPDKLEYLHDLDKEIMLSYFSLSEAIAGLLGEFCEVVIHSLNAY
                     EKSVIKIINGHHTNRKIGSPITDKGLQLLKCYQKDQSNTELVYFSRLNNGNLVKSTTH
                     IIIGHEGKPIGLFCINLNLSTPLDKTVSSFMNYDPIPPHYKSENFITHPKELIEETFL
                     KAVTHIEQHTTKHSKLYKKSIIFYLFENGIFELKEAVPIVAEKLSLTSHAIYKHLREF
                     KNNT"
BASE COUNT         1631 a         1105 c         1242 g         1518 t
ORIGIN      
        1 agaacattta tataaaaatt ggtaatttaa acaatgacaa cacaaaactt ccttgccgag
       61 atcggcacag aagagctgcc accgaaggca ctcaaaaaat tagcgaccgc atttgcggag
      121 aatgtagaaa acgaattaaa ccaagcaggt ctttcgtttg agaaagtgga atggtttgct
      181 gctcctcgcc gtttggcggt gaaggtgttg agccttgcgg aaagccagcc gagtaaagag
      241 gttgagaaac gagggccggc ggtgtcggca gcgtttgatg ccgaaggcaa gccgactaag
      301 gcagcggaag gctgggcgaa gggctgcggg attaccgtag agcaagctga gcgtattgcc
      361 accgacaaag gcgaatggtt ggtgcatcgt gccgtgattg aaggtcagcc gactaaaaat
      421 ctgttggtcg atattatcgc caaatcgctg gcgaacttgc cgattccaaa aacgatgcgt
      481 tggggcgata aaaccgagca gttcgtccgc cctgtgcata ccgtgacact tttattcggg
      541 gcggagttga tcgaaggtga gatcttaggc gtgaaaagcg gccgtactat tcgtggtcac
      601 cgtttcttag gtgaacgtga gttccaaatt gagaacgccg atcaatatcc gcaactgttg
      661 aaagatcgtg gatctgtggt agcagatttc aatgagcgta aagccttaat tcttgcaaaa
      721 tcacaggaaa aagcaaccgc tttgggtggc gttgccgata ttgaagaaga tttgctggac
      781 gaagtgacct cgttggtgga atacccgaac gtactcaccg ccaagtttga agagcgtttc
      841 cttgctgtgc cggcggaagc cttagtttac accatgaaag gcgaccaaaa atacttcccg
      901 atttattcaa aagacggcaa attgttaccg cactttattt ttgtgtcgaa catcaatccg
      961 gaagacccaa gcaaaatcat cgagggtaac gaaaaagtgg ttcgcccacg tttgaccgat
     1021 gcggagttct tcttcaaaac cgacttaaaa cagaagttgg aagaccaact gccacgcctt
     1081 gaaaccgtgt tgttccagca acagcttggc acattgcgtg ataaaaccgc tcgcattgaa
     1141 gcgttggcag gtgaaattgc gaagcaaatc ggggcagatg aaaccaaagc gaaacgtgca
     1201 ggtctcctct caaaatgtga cctgatgacc aatatggtgt ttgaatttac tgatacccaa
     1261 ggcgtgatgg gtatgcacta tgcccgccac gacggtgaag atgaagaagt ggcggtggcg
     1321 ttaaacgagc agtatatgcc acgttttgct ggcgataact tgccaaattc attggtggct
     1381 tgctcggtgg cgttggcgga caaatttgac acgctaacag ggattttcgg cattggtcaa
     1441 gccccgaaag gcagtgccga cccgttcgcc ctacgccgtg cggcacttgg ctcgttgcgg
     1501 attattgtgg agaaaaattt accgcttgat ttaaccgatt tagtggcgaa atctgctcaa
     1561 ttatttggcg ataaactcac caacaaagac gtggttgagg acgtggtgga ctttatgctc
     1621 ggccgcttcc gtgcgtggta tgagagcgaa ggcattgcgg tggacgtgat ccaagccgtt
     1681 ctcgcccgcc gtccgaccaa gcctgccgac ttcgatgccc gtgtgcgtgc cgtgtctcac
     1741 ttccgcaccc ttgacagtgc cgaagcctta gccgcagcga ataaacgggt gagtaacatt
     1801 cttgccaaag ccgatattgc aattggcgac attgatgttt cagcttgcgt agagccagca
     1861 gaaaaagcat tagccgaggc ggttattgcg ttgaaagccg aagttcaacc gctgattgca
     1921 cagggcgact acaccgctgt gctggataaa ttagcgaacc tccgccaacc ggttgatgcc
     1981 ttctttgacg gagtaatggt aaatgcagaa gaccaaaaac tgcgtcaaaa ccgcctcgcc
     2041 attttaagca cgctacaagg cttgttcctg caagtggcgg atatttcgct gttgcaataa
     2101 caataagttt gcaataattg caaaatttta atgaaaatag accgcttgca gtaattgtga
     2161 tctgcaagcg gtcttttttg cttgaaattt taccaaaggc gggattattt aagttttggt
     2221 ttattcacca taatctcgat gacttggcgg tctgtgtatt gcatactacg agaggcaatg
     2281 ctgcataagt tattaattga acgatcaata tcgtgttcga caattccttc gttgcctgtt
     2341 acttggctat tatccataga catcagaatg gatttatagc ttgatgttac gctggtggag
     2401 actttcatcg cacaactgtt tgaagcacca tcacagataa taccgctaat atcgccgatc
     2461 atactactaa ttgccatact gatattctca aatttccctg tgagcagcca cgctatgccg
     2521 gcacagcttc ccatagccgc ggttgttacg gcacagagtg cagataattt cggtaacttg
     2581 ctgtgaatat aaattgccat gaggtgtgat aaaaagagtg ctctaatgcg ttgctcatca
     2641 gtgacattta aatggcgggc aacgacaaca atcggcatag ttgctgtaat tccttgatta
     2701 cctgaacccg aattgctcat agcaggtaaa cttgcaccgc ccattctagc atcagaggca
     2761 gaggtggttt ctataacaat gcgattaagc aaatcatcac tgattagacc tagaccaatc
     2821 tgtttttgca atgtccttcc gatatgtagc ccataatcgg cacgtaatcc ttcttgtgag
     2881 agtgcggaat ttaattgtgc tgcttgctca ataaagcgaa ttttttccaa ttcgatttgc
     2941 atagaaaaat taaatatatc ttttgccgaa agtgaagcaa agatctcagt agtattatcg
     3001 ttattgacgg cacaaggttt gcttaaaata gtttgcccgt ttttttcaat gtaaatgaca
     3061 ttggtatggt gttcagcaat acgaacacat actcgattcg cttggtgaaa tagtgttgct
     3121 tcagaatata gaatatgttc tgtttcgaaa atagagacag acactttatc ggtatttaac
     3181 atagctttag cttgcttaac ctgttctggc gtaatatttt ttaatacttc taatccacca
     3241 tcaggctccc ctccgattgc tccaatagct gccgccatag gcaatccgac ggttgcagtt
     3301 cccggtactg ctactcccat cccatttttc atcaagttag gggaaacttt tgcctcaata
     3361 cgttctggta aatgacctaa atattggcgt gcaatggcgg atgcaagggc gagagaaata
     3421 ggttctgtac agcctaatgc agggacaact tcatggttga ctgcatgtaa aatggattgt
     3481 actaattttt cgcagtgaat attcatagat aaaaccttat tactctaaaa atgtgaggaa
     3541 tggagaaata cataataaaa tgccggtaat gataataata ttgagtgcta gccctttata
     3601 tttgaataat gcaggcactt tatagactaa ataggccgga atcaagcacc caactaagcc
     3661 aaaaatagga ctacagattg aggtaaagct taaaaccggt gcatttaata caatcgcact
     3721 ccaagccagc agaatggaaa ataacataat ccctttctgc acataattag gattgatttt
     3781 ttcagccggg aattttctta ataataggtt catcacaatg ccttgagtgg cttctctaaa
     3841 acctaaatat accccgaaaa aggctgtcat aacggcaaaa atatttaatg caacacctac
     3901 ataggttgcc caactaccag ggaaaaattg ggctgcaatt gctaatgcgg aaacattttg
     3961 ctcgtatgct ttgagcgctt cttctctccc cattgcaagt gtaaaagaga cagcgtaaaa
     4021 aaaaacggta ataaataaga tagcaaatgc aatattcatc gctcgtaggg ctttatagcg
     4081 cgcaacttca cgagactgtt ctttagcacg gtaagaaatt accattgggc tgagagtttg
     4141 caagaagagt atagaggtta gggtgaaagg aagggtaatg atggcatctt ttataagttt
     4201 gccgaaagga ggcattgcac cgatattatg gagttgccac attccaatca ttgcaagccc
     4261 taaggcggca acgacaaata gcttagttaa taccataaat gtagatattt taaacagtag
     4321 cttttcgcct cgagaagata aggcaactaa tatacagatt agcactaagc cataaaatgg
     4381 attatcagag agtagtgtat tggtaatgcc gaaggtaaga agataggagg cactgtcgtt
     4441 tgtaattgcg gtagaataaa caaacatcca aatcactaac ataataaaat aaagtacacc
     4501 taaaaagatt ccccagtttt tgcccaggta gccactaata acggtggggt aatctttgca
     4561 ttccggagat tctgctaaag tattaatgaa cagacgctga aaaaggtaca tcgccgggta
     4621 tccgataata gaagaaagta gaaaaaccca catgcccata agtcctactt gtaccggtaa
     4681 aaagacgatg cctgccccga tagccatacc aatactcata atgatccagc cgaagtcggt
     4741 agaatcaaat tttgttgctt gtttaaattc ggcaggcgag agtttttgag tatccataga
     4801 gtctccttaa atcgtaacag tataattaag tgttattttt gaattctctt aaatgtttat
     4861 aaattgcgtg gctggttagg gagagttttt ctgccacaat aggtactgcc tcttttagct
     4921 caaagatgcc attttcaaac aaatagaaga taatggattt cttatagagt tttgaatgtt
     4981 tggtagtgtg ttgctcaatg tgagtaactg cttttagaaa ggtttcttca ataagttctt
     5041 ttgggtgagt aataaagttt tcacttttat aatgaggcgg aataggatcg taattcataa
     5101 aagagctaac ggttttatct aatggtgtcg ataaatttaa attaatacaa aataatccga
     5161 taggtttgcc ctcatgcccg ataataatat gcgtggtgga cttaactaag ttaccgttat
     5221 ttaaccgtga aaagtaaacc agctcagtat tagattgatc tttttggtaa cattttagta
     5281 actgtaatcc tttatctgta ataggcgaac ctatttttcg gttagtatga tgtccgttaa
     5341 tgattttaat aacggatttt tcataggcat tcaatgaatg aataaccacc tcacagaatt
     5401 ctcctaataa ccctgctatt gcttcactta atgagaaata agacagcata atttctttgt
     5461 ctaaatcatg aagatactct aatttatctg gcataa
//
LOCUS       DANLRK010000169         5438 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.169, whole genome shotgun sequence.
ACCESSION   DANLRK010000169 DANLRK010000000
VERSION     DANLRK010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5438
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.169"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            85..603
                     /gene="hutZ"
                     /locus_tag="PXA33_002205"
     CDS             85..603
                     /gene="hutZ"
                     /locus_tag="PXA33_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme utilization protein HutZ"
                     /protein_id="HDL5121505.1"
                     /translation="MTTNRQEVLQNRLGPEIEELKQQIKTIMLATIDKDGVPNVSYAP
                     FVINNGEYQVLITTIARHARNLQEVPKVSLMLIEDESKSRQIFARRRLTFDATVRMIE
                     RTDEEWNNSIAALKARHGDLVDELSNMQDFKLFSFKPTAGLFVKGFGKAFDVGVDDLI
                     NVVHLDQGHQTA"
     gene            753..3365
                     /gene="pepN"
                     /locus_tag="PXA33_002206"
     CDS             753..3365
                     /gene="pepN"
                     /locus_tag="PXA33_002206"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HDL5121506.1"
                     /translation="MQPKAKLRKDYRAPDFTITDIYLDFQLDPDRTFVTSKLTVVRKN
                     PEATTLRLDGHSFDFLSLKYNGEPFSAFQKDDESLTLNLADVPAERFELEIETALNPA
                     QNTSLQGLYKSGDGFCTQCEAEGFRQITYMLDRPDVLAKYTTKITASKSKYPYLLSNG
                     NHIAQGDLHDGRHWVEWQDPFFKPSYLFALVAGDFDLLQDKFITKSGREVTLEIYVDR
                     GNLDRAPWAMESLKRSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKF
                     VLAKPETATDTDYLDIEAVIAHEYFHNWTGNRITCRDWFQLSLKEGLTVFRDQEFTSD
                     LWSRSAKRIEDVRLLRTVQFAEDASPMAHPIRPEKVIEMNNFYTVTVYEKGAEIIRMI
                     HTLLGEEKFQQGMQLYVARHDGSAATCEDFVKAMEDASGVNLEQFRRWYSQSGTPELT
                     ISDEYDETRKAYRLHISQMTPATHDQMDKLNLHIPLKVALYGEQDGKRIPLQYELLTV
                     SDVLDVTAEHQTFEFHNVTQRPVPALLCDFSAPVRLDYNYTTEQLLTLLKHAENDFVR
                     WDAAQMLYTNELRENLSRYQQHQPLSFSEGLVNALTFVLDNSASLPELTALTLTLPKE
                     TEFAESFKTIDPQGIAVVREFMQHSIAHALQDKLLAVYHQNQCGEYQVVAEDIAKRAL
                     RNACLSYLAFTDLGNALVHKHYNNADNMTDTLAALQAATKAQLSCRDALLADFEQKWK
                     HDGLVMDKWFMLQATRPDDDVLSIVQNLMEHPSFNFNNPNRLRSLVGAFCGQNPKAFH
                     AIDGSGYRFLVDTLIKLNESNPQVAARLIEPLIKLSRYDNQRQTLMRRGLERLRELDN
                     LARDLFEKIEKALD"
     gene            complement(3425..3697)
                     /locus_tag="PXA33_002207"
     CDS             complement(3425..3697)
                     /locus_tag="PXA33_002207"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HDL5121507.1"
                     /translation="MVAKLFSVYGRVQGVAFRFFTLQEANRLGIKGYVRNRDDGSVEV
                     VAEADEQTIAQLENWLQQGSPAAKVERVIVSDYLGSERFKRFEVRY"
     gene            complement(3751..5385)
                     /locus_tag="PXA33_002208"
     CDS             complement(3751..5385)
                     /locus_tag="PXA33_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005700110.1"
                     /note="is involved in the transport of the murein peptide
                     L-alanyl-gamma-D-glutamyl-meso-diaminopimelate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HDL5121508.1"
                     /translation="MQSKFSLSLLTTSLVLGLSANVFAAKVPEGTTLAEKQEITINNG
                     FEPSSFDPHKIEGVPESQVAYQLFEGLVTKDSDGNIQPGVAESWENTPDYKTWTFKLR
                     QDAKWSNGDAVTAHDFVFAWQRLVNPLTASPYSSYLTYLNVENAQDIIDGKKKPEELA
                     VKAIDDHTFQVQLSQPVPYAVELATHSSLLPVHKATVEKHGDAWVQKGNLVGNGAYTL
                     ADHVINEKIVFERNKNYWNDKETVINKATFLAIANAATDVSRYRAGDLDMTNYALPPE
                     QFATLKKEIPNEVFTARTLSTYYYEINHKRAPFDNVKVREALNLALDRTIITDKVLGQ
                     GQTPTYVFTPPYIHEGEKIQQPAYSKDSMADRKAKALKLLEEAGYSKANPLKFTILYN
                     TNDNHKKIAIAVSSLWKQGTDGIVDVKLENQEWKTFLDTRRNANYDVSRAGWSADYNQ
                     ATTFVNYFLSNSSNNTAFYKSQAYDDAVAESYKATDAAGRAAAYAKAEEQLAKDYAII
                     PIYNYVNPRLVKPYVKGYEGKDPQDDILLRNLYIVK"
BASE COUNT         1583 a         1171 c         1085 g         1599 t
ORIGIN      
        1 tagtcaataa tgattctcat atggattgaa agttttcact ctttatagta atctaaaaaa
       61 acactattta aattgaggta ttttatgaca accaaccgtc aagaggtctt acaaaaccgt
      121 ttaggtccag aaattgaaga attaaaacaa caaatcaaaa ctataatgct tgcaaccata
      181 gataaagatg gtgtaccgaa tgtgagctat gccccttttg taattaataa cggcgaatat
      241 caagtcttaa tcactactat tgcccgtcac gctcgtaatt tacaagaggt gccaaaagtc
      301 tctttaatgc taattgagga tgaaagtaag agccgtcaaa tttttgcccg tcgtcgctta
      361 acctttgacg caacagtgcg aatgattgaa cgtactgatg aggaatggaa taatagcatt
      421 gcagcactta aagctcgtca cggcgattta gttgatgaac tgtcaaatat gcaagatttc
      481 aagctcttta gctttaagcc aacggcaggc ttatttgtaa aaggatttgg taaagccttt
      541 gatgttggtg ttgatgactt aattaatgtc gttcatttag accaaggcca ccaaactgcc
      601 taattgcgaa ataaactcac ggctataaat aatcctattt atagccgttt ttattgcgac
      661 aagcggtcaa attttttgga aaatttaccg ctattttagt acaataacga cactattcta
      721 tgagctaaac gcaactaaaa aaaggaaatc ctatgcaacc taaagcgaaa ctcagaaaag
      781 attatcgagc tcccgatttc actattactg atatttatct tgacttccaa cttgacccag
      841 atcgcacttt tgtcacctca aaactaactg tcgtgcgtaa aaatcctgag gcgacaacac
      901 ttcgtttaga cggtcatagt ttcgatttcc tttcgcttaa atataacggc gagccgtttt
      961 ccgcctttca aaaagatgat gaatctttaa ccttaaattt agccgatgtg cctgccgagc
     1021 ggttcgagct tgaaattgaa accgcattaa atccggcaca aaacacctcg ttacaaggtt
     1081 tgtataaatc gggcgacggg ttttgcaccc agtgtgaggc tgaaggtttc cgccagatta
     1141 cctatatgct cgaccgccct gatgtacttg caaaatatac gacaaaaatc accgctagta
     1201 agagcaaata cccgtatctg ctttctaacg gcaaccacat tgcccaaggc gacttgcacg
     1261 atggcagaca ctgggtggaa tggcaagatc cgttctttaa accaagctat ttatttgcct
     1321 tggttgcggg ggattttgac ttactccaag ataaattcat caccaaaagc ggacgtgaag
     1381 tcacgcttga aatttatgtc gatcgtggaa atttagaccg tgcaccttgg gcgatggaga
     1441 gcttaaaacg ttcaatgaaa tgggatgaag agcgtttcgg tttggaatac gatttagata
     1501 tttatatgat tgttgccgtt gatttcttca atatgggagc aatggaaaac aaagggttaa
     1561 atattttcaa ttccaaattt gtattggcaa aaccggaaac cgcaaccgat acggattatt
     1621 tagatattga agcggtgatt gcccacgaat acttccataa ttggacgggc aaccgcatta
     1681 cctgccgtga ttggttccaa ctaagcttaa aagagggctt aaccgtcttc cgtgatcagg
     1741 aatttacctc cgatttatgg tctcgttcgg caaaacggat tgaagatgtg cgtttactgc
     1801 gtaccgtaca atttgccgaa gatgccagcc cgatggctca cccgattcgc ccggaaaaag
     1861 tgattgaaat gaataatttc tacaccgtga ctgtgtatga aaaaggggcg gaaattattc
     1921 gtatgattca caccctgtta ggcgaagaaa aattccagca aggtatgcaa ctttatgtgg
     1981 caagacacga tggttctgcg gcaacttgtg aagatttcgt taaagcaatg gaagatgctt
     2041 caggcgttaa tcttgaacaa ttccgccgtt ggtatagcca atccggcacg cctgagctca
     2101 ccatttccga tgaatatgat gaaacccgca aggcataccg cttgcatatt tcacaaatga
     2161 caccggcaac ccacgatcag atggataaac tcaatctgca tattccgctt aaagtagcgt
     2221 tatatggtga acaggacggt aaacgcattc cgttacaata tgaattattg accgtaagcg
     2281 atgtgttaga tgtaaccgca gaacaccaaa cctttgagtt tcataatgta acacaacgcc
     2341 cggtgcctgc attactgtgt gatttctctg caccggtacg gttagattac aactatacca
     2401 ccgagcagct tttaacctta ctcaaacacg ctgaaaatga ttttgtacgc tgggatgcag
     2461 ctcaaatgct ctataccaat gaactgcgtg aaaatttaag ccgttatcag caacatcaac
     2521 cactcagctt ctcagaaggg ttggtgaatg ccctaacgtt cgtgttggat aactcagcaa
     2581 gcctgccgga actaactgca ttaaccctca cattgccaaa agaaaccgag tttgcagaat
     2641 cgttcaaaac cattgaccca caaggtattg cggttgtgcg tgaatttatg cagcattcca
     2701 ttgctcacgc cttgcaagat aagttattag cggtatatca ccaaaaccaa tgcggggaat
     2761 atcaagtcgt ggctgaagat attgctaaac gagccttgcg taatgcttgc ttaagctatt
     2821 tagcctttac tgatttaggc aacgcgttgg tgcataagca ttacaataat gccgataata
     2881 tgactgatac gcttgctgca ttacaagccg caaccaaagc ccagctaagt tgccgtgatg
     2941 cattattagc cgattttgag caaaaatgga aacatgatgg tttagtgatg gataaatggt
     3001 ttatgctaca agccacccga ccggacgacg atgtgttatc catcgtgcaa aatttaatgg
     3061 aacacccaag ttttaacttt aataacccga accgtttacg ttcattagta ggtgcattct
     3121 gcggtcaaaa cccgaaagcc ttccacgcca ttgacggttc aggctatcgt ttcttggtag
     3181 atacgctgat taaacttaac gaaagtaacc cacaagttgc tgctcgactt attgagccgt
     3241 taattaaact ctcacgctac gataaccaac gccaaacctt gatgcgtcgt ggtctagaac
     3301 gcctgcgtga gctggataat ttagcacgag atctgtttga gaaaattgaa aaagcgttag
     3361 attaaaccaa gcaatacaag cggtgtcgtt tgcaaatttt tttacaaatg acaccgcttg
     3421 ttagttaata ccttacctca aaccttttaa acctttctga gccaagataa tcgctcacaa
     3481 ttacccgttc cacctttgca gcaggtgagc cttgttgtaa ccaattttcc agttgagcga
     3541 ttgtctgttc atccgcttca gctaccactt ctaccgagcc gtcatcacga tttctgacat
     3601 agccttttat gcctaatcga ttcgcttcct gcaaggtaaa aaaccgaaaa gcgacacctt
     3661 gaacccgtcc gtaaaccgag aataattttg cgaccattct tcccccataa aaataggctg
     3721 cttatttttc aataaacagc ccataatgtc ttatttcacg atgtagaggt tacgcagtaa
     3781 aatatcatct tgcggatctt taccctcata gcctttcacg taaggtttca ccaaacgtgg
     3841 gtttacatag ttataaatcg gaataatcgc ataatctttg gctaactgct cctctgcttt
     3901 tgcataagca gctgcacgac cagctgcgtc tgtcgctttg taagattctg ctaccgcatc
     3961 atcataagct tggctcttat aaaacgcagt attgttgctg gagttcgata agaaatagtt
     4021 cacaaaggtg gttgcttggt tatagtctgc cgaccaacct gcacgagaaa catcataatt
     4081 tgcattgcgg cgggtatcta agaatgtttt ccactcttgg ttttctaatt tcacatcaac
     4141 aatgccgtct gtgccttgtt tccataatga tgaaactgca atcgcaattt tcttgtggtt
     4201 gtcgttggta ttataaagaa tagtaaattt taacgggttc gctttactgt aaccggcttc
     4261 ttccaataat ttcaacgctt tggcttttct atctgccatt gaatctttgg aataagcagg
     4321 ttgctggatt ttttcaccct cgtgaatata aggcggtgta aatacataag ttggtgtttg
     4381 accttgtcct agcactttat cggtaataat ggtgcgatct aaagctaagt ttaacgcctc
     4441 acgcactttc acattatcaa acggtgctcg tttatggtta atttcatagt aataagtaga
     4501 aagggttctt gccgtaaaca cttcgtttgg aatctctttt ttcaaggttg caaactgctc
     4561 cggtggaaga gcatagttgg tcatatccaa atcaccggca cggtaacgtg aaacatcagt
     4621 tgcggcatta gcaatggcta agaaagtcgc tttattaatc acggtttctt tatcattcca
     4681 ataattttta ttacgctcaa acacgatttt ttcattaata acgtgatcgg ctaaggtata
     4741 agccccgttt ccaaccagat tgcctttttg cacccacgca tcgccgtgtt tctcaacggt
     4801 tgctttgtgt acaggtaata aagaagagtg tgttgccagc tcaactgcat acgggacagg
     4861 ttggcttaac tgcacctgga aagtatgatc atcaatcgct ttaactgcta gttcctccgg
     4921 ttttttcttg ccgtcaataa tatcttgagc attttctaca ttgagataag ttaaatagct
     4981 tgaataaggt gaagccgtaa gtggattaac caaacgctgc caagcaaata cgaaatcgtg
     5041 agcagttacg gcatcaccgt ttgaccattt agcgtcttgg cgtaatttga acgtccacgt
     5101 tttgtaatcc ggtgtatttt cccaactttc tgccacaccc ggctgaatat tgccgtcaga
     5161 atctttcgtt accaatcctt caaataactg ataagcaact tgcgattccg gcacgccctc
     5221 aattttatgt ggatcaaaac ttgatggctc aaaaccatta ttaatcgtaa tttcttgttt
     5281 ttctgcaagc gttgttcctt caggcacttt ggctgcaaac acatttgccg ataagcctaa
     5341 cactaatgag gttgttaata aggaaagcga gaattttgac tgcatatttg atctccattg
     5401 atgaatattg gcttgcaacc agccgatttg ttctaatt
//
LOCUS       DANLRK010000170         5228 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.170, whole genome shotgun sequence.
ACCESSION   DANLRK010000170 DANLRK010000000
VERSION     DANLRK010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5228
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.170"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            54..200
                     /locus_tag="PXA33_002209"
     CDS             54..200
                     /locus_tag="PXA33_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121509.1"
                     /translation="MLAEKPKKRWRFWYAFVNGLFFDKKHCYAAYKAEVYRKQYPPEF
                     TEIT"
     gene            307..1488
                     /locus_tag="PXA33_002210"
     CDS             307..1488
                     /locus_tag="PXA33_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath protein"
                     /protein_id="HDL5121510.1"
                     /translation="MSILDTYLHGVEVVEVNAGGVTISTAATSVIGVVCTGDQADAET
                     FPLNTPVLITNPLNYLEKAGSTGTLRRTLNSIGSIVKTPTVIVRVAESDDSDTLTANI
                     VGTQENGKFTGIKALLTAQSTVFVKPKLLCVPQHDNQAVATELLSVAKKLNAFAFISD
                     NGATTKEQAYTYRQNFSQREGMMIFGDWKSYNTDKKAYDTDYAVARACALQAYIDKTV
                     GWHKNISNVELDGVTGITKAVEFDINESSTEANYLNEKGITICLNHNGFRYWGSRTLA
                     TDTRWAFQQSVRTAQIIKETIGAGLAWAVDMPLTPLRVKTMLEAINNKLRSWASGDDP
                     RILGARVWVAEEITADIIKSGKFVIKYDYHWIPSLESLGLEQRVNDEYVVDLVNTLKA
                     L"
     gene            1497..2003
                     /locus_tag="PXA33_002211"
     CDS             1497..2003
                     /locus_tag="PXA33_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HDL5121511.1"
                     /translation="MGLPAKLKNFNFSVDGTSYLGETTEVTQPKLAMQLEDYRAGGMI
                     APVGVNMGLEKLELEFKIGGHEEDLLKLFGGSISGNAFRFNGAYQHDDDDSVDAVELV
                     CRGRIAEIDEGSSKAGDDTEHSYKASLTYYKKTVNGVDIIEIDTLNQIYIVDGKDRLA
                     EIRKAMGL"
     gene            2082..2396
                     /locus_tag="PXA33_002212"
     CDS             2082..2396
                     /locus_tag="PXA33_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HDL5121512.1"
                     /translation="MKKVTLKQGILRGEKRITEIEVRKPLTKQLRGTNLTSLMQLSVD
                     EWCIVLPRITTPKLDKADFATMSAADLLKLSGKALDLMSEDFDEADEEDSEEQGKGEI
                     LA"
     gene            2420..2536
                     /locus_tag="PXA33_002213"
     CDS             2420..2536
                     /locus_tag="PXA33_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HDL5121513.1"
                     /translation="MADIATVFHWTPNTFDEMTIVELGQWREKARLRNGVES"
     gene            2600..2947
                     /locus_tag="PXA33_002214"
     CDS             2600..2947
                     /locus_tag="PXA33_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121514.1"
                     /translation="MEIKTVTFSKVITAFIFHIITLFVFTQLNEQAQATFMFFWWFFI
                     SLRFILVVGIMCVGIEELKIQEYRQTKAYACFSFISNFALCLQFVYFEHLIAAILFLI
                     SYIIYHSQTVTIK"
     gene            2949..3386
                     /locus_tag="PXA33_002215"
     CDS             2949..3386
                     /locus_tag="PXA33_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL5121515.1"
                     /translation="MLQQFAMMSLGYFVFMRLTIPYQDTSREMNWSHVQNDVVGAMPK
                     SQFTGKAGETMEISAELRPEITGGKMSILALEMMAEQGSAYPLISGSTFMVLGWFVID
                     RISEQETTFFADGTPRAISFSMSLKRVDDSLLANIIDEVAGFI"
     gene            3386..4624
                     /locus_tag="PXA33_002216"
     CDS             3386..4624
                     /locus_tag="PXA33_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HDL5121516.1"
                     /translation="MDFLTELTNHNHRTPAVSVTVRPKPSKDNEGEKAKDISTLITHR
                     LIQLTLTDNRGFEADQLDLELDDTDGLLALPSRGAILSVGLGWQNSPLTYKGEYTVDE
                     LTHDGPPDKVTIRARSADLRGTLTNRHERSFHRTTIGKIVKQIAEENKLKPMVGKEFE
                     NEEVKHIDQTNESSINLLQRLAEQFDAIATVKNGNLIFIKAGNATTASGKLLPLFRIT
                     RSSGDSHSFSIAEGDNYKAVKAYWHNTQTGKRGEVTWDENSQVKKVTKPTMRKKTKVK
                     RGADGKPIKGKDGKSIKETVFVKGKGRQVNAVVQSKPIESDSEAIKTLPHTYATAQSA
                     INACKRNFEKLQRGVATFSLTLAEGNAELIPELPVQVSGFKAEIDSNEWIISQVTHSL
                     NKGGGFTTALEMELKPKSED"
     gene            complement(4807..>5228)
                     /locus_tag="PXA33_002217"
     CDS             complement(4807..>5228)
                     /locus_tag="PXA33_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121517.1"
                     /translation="FVLSVDDLPFKYNKADEAMHIIANNIIRKKTAVANAILALNEVD
                     SRKSSGVQLCDLLLGAILSSYQNDITSERKKELTKWIALHLGWDKLTYDTLQEEKKFN
                     IWYFYDPTKGPRIVRTRETKLKYPLLKKKEQTFQPAR"
BASE COUNT         1558 a         1106 c         1184 g         1380 t
ORIGIN      
        1 caatttaaac aagctccgct accttttcca gcagccgttg ttaccgtggt gcaatgcttg
       61 cggaaaagcc gaaaaagcgt tggcgttttt ggtatgcgtt tgtgaatggc ttgttttttg
      121 ataaaaagca ttgctatgca gcctataaag cggaggtgta tcgaaaacag tatccaccgg
      181 agtttaccga aatcacttaa tcctgttaaa ccccatttca cagcccgaac cgctcgcaac
      241 ccgagcggtt attttttaaa gtttacccca acattcaacc ctaacctttc ccaattaagg
      301 aatactatgt caattttaga tacctatcta cacggtgttg aggtggttga ggtcaatgca
      361 ggtggtgtca ccatttccac cgctgctact tcggttatcg gagtagtttg caccggagac
      421 caagccgatg ccgagacttt tccgctgaat acgcccgttt taattactaa cccgttaaat
      481 taccttgaaa aagcaggtag caccggcacg ttacgccgca cacttaattc gattggctca
      541 atcgttaaaa cgccaacggt gatcgtgcga gttgccgagt cggacgatag cgacacctta
      601 actgcaaata ttgtcggcac gcaggaaaac ggcaaattta ccggtattaa ggcgttatta
      661 accgctcaat caaccgtgtt tgttaagccg aaattgcttt gtgtaccaca gcacgataat
      721 caagccgttg cgaccgaact attaagcgtg gctaaaaagc tgaatgcgtt tgcgtttatt
      781 tcagacaacg gtgcaaccac caaagaacag gcatatacct atcgccagaa tttctctcag
      841 cgtgaaggaa tgatgatctt cggtgactgg aaatcttata acactgataa aaaagcctat
      901 gataccgatt atgcagtggc ccgagcttgt gcgttgcaag cctatatcga caaaactgtc
      961 ggctggcata aaaacatctc aaacgtagag ctagacggtg taaccggtat cactaaagcg
     1021 gtagagtttg atattaacga gagctcaacc gaggcaaact acctgaacga aaaaggcatt
     1081 accatttgct taaaccataa cggtttccgc tattggggtt cccgcactct tgccaccgat
     1141 acccgctggg cgtttcagca gtcggtgcgg acggctcaaa tcattaaaga gacaatcgga
     1201 gcaggcttag cttgggcggt ggatatgcca ctcacaccgt tgcgtgtgaa aacaatgcta
     1261 gaggcgatta acaacaagct acgctcttgg gcatcgggtg atgaccctcg cattttaggt
     1321 gctcgtgtgt gggtagctga agagatcacc gcagatatta tcaaatcagg caaatttgtt
     1381 attaaatacg attaccattg gattccgtcc ctcgaaagcc taggcttaga gcaacgggtc
     1441 aatgatgaat atgtagtgga tttagtcaat acacttaaag cgttatagag gtaaaaatgg
     1501 gattaccggc aaaacttaag aattttaatt tttcggtgga cggcacaagc tatttgggcg
     1561 aaaccaccga agtcacacag ccgaaactgg ctatgcaact tgaagactac cgagcaggcg
     1621 gaatgattgc ccctgtgggt gtcaatatgg gtttagagaa actggagctt gagttcaaaa
     1681 tcggcggaca cgaagaggat ttgctcaagc tatttggtgg ctcaatcagt ggtaatgcgt
     1741 tccgtttcaa cggtgcgtac cagcacgacg atgacgattc agtcgatgcg gtagagttag
     1801 tctgccgtgg gcgaatcgct gaaattgacg aaggctcaag caaagccggt gatgacaccg
     1861 agcatagcta taaagcctcg ctaacctatt acaaaaaaac ggtgaacggt gtcgatatta
     1921 tcgaaatcga caccctcaac caaatttaca ttgtcgatgg caaagaccgc ttagcggaaa
     1981 ttcgtaaggc aatgggttta tagattttcc cctaaatttt cccaaagccc cttccccttt
     2041 tttcaagggg cttttaatga taaataatta aggattaaac aatgaaaaaa gtaacattaa
     2101 aacaaggcat tttgcgtggt gaaaaacgta ttaccgagat tgaggtgcgt aagccattaa
     2161 ccaaacaact gcgtggcaca aatcttacct cattaatgca gttaagcgtt gatgagtggt
     2221 gtattgtact gccacgcatt accacgccaa aattagacaa agccgatttt gccacaatgt
     2281 cggcagcgga tttgctaaaa ctctccggca aagccttaga tttaatgagc gaagacttcg
     2341 atgaggcaga cgaagaggac agcgaagaac agggaaaggg cgagatttta gcctaattcc
     2401 acagtttgtt gatgacgcta ttgctgatat tgctaccgtg tttcattgga cacccaacac
     2461 ctttgatgaa atgacgattg tcgagctagg gcaatggcga gaaaaggccc gcttacgaaa
     2521 tggggtggaa agttaaaaca agcggtcgtt tttcgcaaaa aatttgcaaa actcgaccgc
     2581 ttgctataag gattttaaaa tggaaattaa aacagttaca ttcagtaaag tgattacagc
     2641 attcattttt catatcatta cgttatttgt ttttactcaa cttaatgagc aagctcaagc
     2701 tacctttatg ttcttttggt ggttcttcat ttctctgcga ttcatccttg tagttggaat
     2761 aatgtgtgtt ggtattgaag aacttaaaat tcaggaatac cgacaaacaa aagcttatgc
     2821 ttgttttagt ttcatatcca attttgcgtt atgcttgcag tttgtttact ttgagcatct
     2881 tattgcggca attttatttt tgatttctta catcatatat catagccaaa ctgtaacgat
     2941 taaataatat gctccaacaa ttcgcaatga tgtcgctagg ctattttgtg tttatgcgat
     3001 tgacaatccc ttatcaagac accagccgag aaatgaactg gtcgcacgtt caaaatgatg
     3061 tggttggggc aatgccaaaa agccaattta ccggcaaggc aggggagaca atggagattt
     3121 cagccgagct acgccctgaa attaccgggg gcaaaatgtc aatccttgcc cttgagatga
     3181 tggcagagca aggctcggct tatccgttga ttagtggttc aacctttatg gtgctaggct
     3241 ggtttgtgat tgatcgaatc agcgagcagg aaaccacctt ttttgcagac ggcacgcccc
     3301 gggcaattag cttttcgatg tcgcttaaac gggttgatga tagtctgctc gccaatatta
     3361 ttgatgaggt cgcagggttt atttaatgga ttttttaaca gagctaacca atcataacca
     3421 ccgcacaccg gctgtttcgg tgacggttcg cccaaagcca agcaaagata atgagggcga
     3481 aaaagccaaa gatatttcca ccctgatcac gcaccgctta attcaactca ccttaaccga
     3541 caaccgaggc tttgaggcag accagctgga tttagagctg gacgacactg atggcttact
     3601 cgctttgcca agtcgaggag cgattttatc ggtcgggtta ggctggcaaa acagccccct
     3661 gacctacaag ggcgaataca cggtggacga gctaacccac gatggcccac ctgataaagt
     3721 cacgattcgg gcaagaagtg ctgatttgcg aggcacgctg accaatcgcc acgagcggag
     3781 ctttcaccgc accaccattg gtaaaattgt gaaacagatc gccgaagaga acaaactcaa
     3841 gccgatggtg ggcaaggagt ttgaaaacga agaggtgaaa cacattgacc aaacaaacga
     3901 aagctctatt aatctcttgc aacgcctcgc agaacaattt gatgccattg ctaccgtgaa
     3961 aaacggcaat ttgattttca ttaaagccgg aaacgccaca accgcaagcg gtaaactgtt
     4021 acccctgttt cgcattaccc gcagttcggg ggattcccat agcttttcga ttgccgaagg
     4081 ggataactac aaagcggtta aagcctattg gcacaacacg caaaccggca agcgaggcga
     4141 ggtgacgtgg gacgaaaaca gccaagttaa aaaagtgact aagcccacaa tgcggaaaaa
     4201 gactaaagta aaacgtgggg cagacggcaa gccgataaaa ggcaaagacg gcaagagcat
     4261 taaagagacc gtctttgtaa aaggcaaagg caggcaagtt aatgcagtgg tgcaaagtaa
     4321 accgattgaa agtgatagcg aggcaattaa aaccctaccc catacttacg ctaccgcaca
     4381 atcagcgatt aatgcttgta aacggaattt tgaaaaacta caacgaggtg tggcaacttt
     4441 tagcctaact cttgccgagg gcaatgcgga gcttatcccc gaattaccgg ttcaggtttc
     4501 aggctttaag gcggagattg atagcaacga gtggataatt tcgcaggtta cgcacagttt
     4561 aaataaaggt ggtgggttta cgacagcgtt agagatggag ttgaagccga agtctgaaga
     4621 ctaacgctat ttttataaaa tcactataaa aaagccttgc atctctgatc ttgaacaagt
     4681 aatattaatc aaagttacag atttatttac ataaaacaca aagaatcata ggggcggcta
     4741 ctttgtgtga ttccgcccag tgaggaaatt gggcgttgtt ttacacatat aagtccttga
     4801 ggacactcac cgtgcaggct ggaaggtctg ctctttcttt tttaatagtg ggtatttaag
     4861 ttttgtttct ctcgttctta ctattctagg tccttttgta gggtcgtaaa aataccaaat
     4921 attaaatttc ttttcctcct gtaaagtatc ataagttaat ttatcccaac ctaggtgcaa
     4981 agcaatccat tttgttagtt cttttttacg ttccgatgta atatcatttt gatagctact
     5041 taatattgct cctaataata aatcgcatag ttgaacgcca gatgatttac gagaatctac
     5101 ttcgtttaat gctagaatag catttgctac ggcagtcttt ttacgaatta tattattagc
     5161 gataatgtgc attgcctcat cagctttatt gtatttaaaa ggtagatcat caacagataa
     5221 aacaaacc
//
LOCUS       DANLRK010000171         4989 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.171, whole genome shotgun sequence.
ACCESSION   DANLRK010000171 DANLRK010000000
VERSION     DANLRK010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4989
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.171"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(181..891)
                     /locus_tag="PXA33_002218"
     CDS             complement(181..891)
                     /locus_tag="PXA33_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4189 domain-containing protein"
                     /protein_id="HDL5121518.1"
                     /translation="MFMVIQLALYNLQTYEVGRNATTIFMTTDKVYTGGSTWTYLFAD
                     ADNKITSVREMNGFGDVEKEFKCGEYKEAVTIASKEDLAKRKTQPRLWSSIYMSPEQN
                     MMWVTDNTSKNLENAKQDAFKQCEKNGGRNCQMILSFSNMCLAFAQGYKDGQGFEDFG
                     YGIVSSLAEENALENCNQKGGSSCLLSAPAVCAIPCDILSNNKCMYDQPQIIIPGKNN
                     NQPINPPANGNGEHWLSN"
     gene            complement(860..1006)
                     /locus_tag="PXA33_002219"
     CDS             complement(860..1006)
                     /locus_tag="PXA33_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121519.1"
                     /translation="MIKPLDSVYWRGKDVSEFFARKRLPDTAVQCVKDNELSYVYGYS
                     TSFV"
     gene            complement(1124..2341)
                     /gene="fabB"
                     /locus_tag="PXA33_002220"
     CDS             complement(1124..2341)
                     /gene="fabB"
                     /locus_tag="PXA33_002220"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HDL5121520.1"
                     /translation="MKRVVITGLGVISSIGNNKEEVLESLKAGKSGIEFVPEFAEVGM
                     RSQVAGTIKLNPAELIDRKIYRFMGDAAAYAYLSMKEAIEDSGLTEEQVSNDRTGLVI
                     GAGTGSAHSQLVACDAVRGPRGVKAVGPYAVTKTMASSVSACLATPYKIRGVNYSISS
                     ACATSAHCIGHAMELIQLGKQDVVFAGGAEELSWECATEFDAMGAVSTKYNETPTKAS
                     RAYDANRDGFVIAGGGAVVVVEELEHALARGAKIYAEIVGYGATSDGYDMVAPSGEGA
                     ERCMRQAMATVNGEIEYINVHGTSTPVGDVKELGAIRNVFGDKTPAISSTKSMTGHSL
                     GAAGAHEAIYSLLMLDNNFIAPSINIETLDEQAQGMNIVTERQDKALTTVMSNSFGFG
                     GTNACLVFQRYSK"
     gene            2602..>4989
                     /locus_tag="PXA33_002221"
     CDS             2602..>4989
                     /locus_tag="PXA33_002221"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HDL5121521.1"
                     /translation="MKTLTLTANKITAVEGAAIGANQGSFAQKLNSTKARIDQLNAYR
                     IERAINANNHHFMHVFSLIPLLIHLNHPVLPAYVENAPQGIWHFQLSDYQKQFLVSYR
                     FDEAISADHLGPHPSFDALYSMGSTGTITQTSCSDLDLWLCYPNHFTIEQYKLMELKI
                     NKLQEWAKTFQIDINIYLMNPEQFKSQTYNSSVTDEHSGSAQHFFLLDEFYRSAILLA
                     GKRLLWLHLHKNEYQNVHNNPEIDLTEWIDFGDFSSLSTSEFFGASLWQLYKGINNPY
                     KSAIKILLLECYAHTYPKTKLISKEFKKKLLSDNALEYHFDPYLAMLELVTEHLRSRK
                     EWVKLDRLRVCFYAKAVEGEINNNWRSRDLDELVNQWSWSEKQIQLLQNRQHWKIKQV
                     MNHENMLIDHLLQSYRNLIHFARKFHINPSIMTNDTDILMRKLYSVFEALPGKISLIN
                     HKISSNLAEKAITFIEAKVGSALQPGWYLVNQAPKSRYDSENRYVHYNKSPIKLIAWG
                     YFNGIINAATNLHTVSPNLDIEKLRQFITDLRLSFPIQAPEVTNDDMHHPNEIRNLVI
                     ALNLVQDPTEHIDELPKKVYPSDLFNFGKAQKTLVGSVSIIYRNVWNEIRTQHFDGED
                     AILKALKLVSNRIYQGNSSPQSVNVFCYSKYLRSELRDFVADLVHKCITIQTGCLLQE
                     NEFSTLKVAGKTWQFLFHKKTFSIKQIDEQQAVENLNSFAKTKQNAKSENEKKKEFPQ
                     QISEFASEGFLQFFFEDNVDNSFNVYVVDERNNVESYYNCFGNKEEKIRKINKLQN"
BASE COUNT         1638 a          991 c          812 g         1548 t
ORIGIN      
        1 catttataaa aatgatttaa taagaattaa aaactaagat agaatataaa aatggtttta
       61 aataaaatta aaaataaatt gtaaattttt atttaccaag atttttagca aagttttcac
      121 taaaaatgac cgcttgcagg caataaaaaa gtgcgtttcc gcacttttta ctaaaatgat
      181 ttaattactc aaccaatgct caccgttgcc attagctggt ggattaatcg gttggttatt
      241 attcttacct ggaataataa tttgaggctg atcgtacata catttgttgt tcgacaatat
      301 atcacaggga atagcacata cggcaggggc agaaagaaga caacttgaac cacctttttg
      361 attacaattc tctaatgcat tttcttcagc aagactacta acgataccat acccaaaatc
      421 ctcgaagcct tgaccatctt tataaccttg tgcaaaagca aggcacatat tactaaacga
      481 taagatcatt tggcaattac gaccaccgtt cttttcacac tgtttaaacg catcttgctt
      541 cgcattttct aaatttttag acgtattatc agtcacccac atcatatttt gttcaggtga
      601 catataaatt gatgaccata aacgaggctg tgttttacgc tttgctaagt cctctttact
      661 ggcaatggtt accgcttctt tatattcacc acatttaaac tctttttcga catcaccaaa
      721 accattcatt tcacgaacag aagtgatttt attatcagcg tcagcaaaaa gatatgtcca
      781 agtcgatcca cctgtataaa ccttatctgt tgtcataaat atagtggttg cattacgacc
      841 aacttcataa gtttgcaaat tatacaaagc tagttgaata accataaaca tagcttaatt
      901 cattatcttt tacgcattga accgctgtat caggtaaacg tttacgtgca aaaaattcag
      961 atacatcttt accacgccaa taaacactat ctagtggctt aatcattgta gaacaagcgg
     1021 tcaaaaataa tgacaaaata acaaatagca aagaacttat tttgaaattt ataataatta
     1081 cctcaaaaga aaaaagagca agctttcact tgctcttttt atgttactta gaatagcgtt
     1141 ggaacactaa acacgcatta gtaccaccaa agccgaagct gtttgacatc acggttgtta
     1201 atgctttgtc ttggcgttca gtcacaatat tcataccttg tgcttgttca tcaagggttt
     1261 caatattaat gcttggtgca ataaaattat tatctaacat taataatgaa taaattgcct
     1321 cgtgcgcacc tgctgcacct aatgagtgtc cggtcattga tttggttgat gaaattgctg
     1381 gcgttttatc accaaagaca ttacggatcg cccctaactc ttttacatca cccactggag
     1441 tagaagtgcc gtgtacgtta atatactcga tttcaccatt tacagttgcc atcgcctgtc
     1501 tcatacaacg ctcagcacct tcaccacttg gcgcgaccat atcgtaaccg tctgaggttg
     1561 ccccatagcc cacaatttca gcatagattt tggcaccacg agcaagagcg tgttctaact
     1621 cttccaccac aaccactgca ccaccaccgg caatcacaaa accgtcacga ttagcatcat
     1681 aagcacggct agcttttgtt ggtgtttcat tatatttagt tgaaacagca cccattgcat
     1741 caaactcggt tgcacattcc caagaaagtt cttctgcacc acctgcaaac accacatctt
     1801 gtttacctaa ttggattaac tccatcgcgt gtccaataca gtgtgctgaa gtcgcacaag
     1861 ctgagctgat tgaatagttt acaccacgaa ttttataagg tgttgccaag catgctgaca
     1921 cacttgatgc cattgttttg gttactgcgt aaggcccaac ggctttcaca ccacgtgggc
     1981 cacgtactgc atcacaagct acaagttgac tgtgcgccga gcctgtacca gctccgatca
     2041 ccaaaccggt acgatcgttt gaaacttgtt cttcggttaa gcctgagtct tcgattgctt
     2101 ctttcattga aagataagcg taagccgcag catctcccat aaaacgatag attttacggt
     2161 caattaactc agccggattt aatttaatcg tacctgccac ttggctacgc atacccactt
     2221 cagcaaactc aggcacaaat tcaatgcctg atttacctgc ttttagtgat tccaacactt
     2281 cttctttgtt gttaccgata ctggaaatta cacctaatcc tgtaataacg actcttttca
     2341 ttttgtttcc ttcatcattt tgatctgaaa aatacttgat tcagcttaca cttgttcact
     2401 caaataagct actaaaactt gattatttta cacaatttca ttactggtct gaactctttc
     2461 tatcaagata gcggttgaat ttcagacatt ttttgcaaaa tagggaatga aatttaattt
     2521 cccgattttt taccctatta cataataatc acttttccgc taaaatgcta aacagttcac
     2581 actttagtaa ggaaagctga cgtgaaaaca ttaaccctga cagccaacaa aataacagcc
     2641 gtagaaggcg ctgccattgg ggcgaaccag gggagcttcg cccaaaaatt aaactctact
     2701 aaagcacgaa ttgatcaact taatgcatac cgaatagaac gagctatcaa tgctaataat
     2761 catcatttta tgcatgtttt ctctctcatt ccattgctta ttcatcttaa tcaccctgtg
     2821 ttaccggctt atgttgaaaa tgcaccacaa gggatttggc attttcaact ctctgattat
     2881 caaaaacaat ttctggtttc ttaccgtttt gatgaagcaa tctctgccga tcatttgggc
     2941 ccccatccca gctttgatgc tctttattcg atgggtagca ccggtactat tacccaaaca
     3001 agctgttctg atttagattt atggctatgc tatccgaacc attttacaat tgaacaatat
     3061 aagttaatgg agctcaaaat taataaattg caagaatggg caaagacatt ccaaatcgac
     3121 atcaatattt atttaatgaa cccagaacaa tttaagagcc aaacttataa cagtagcgta
     3181 accgatgaac acagtggttc agcacagcat ttttttctat tagatgaatt ttatcgttca
     3241 gctattttac ttgccggtaa gcgtttactc tggttgcatt tacataaaaa tgaataccaa
     3301 aatgttcaca ataaccctga aattgactta acggaatgga ttgatttcgg cgatttttcc
     3361 agcctttcaa ccagtgaatt ttttggtgca agcttatggc agctctacaa aggaattaat
     3421 aacccttata aatcggcaat taaaatttta ttgctcgaat gctacgctca cacttatcct
     3481 aaaacaaagc tcatctccaa agaatttaag aaaaaactac taagtgacaa tgctttggaa
     3541 tatcactttg atccctactt agccatgctt gaattggtaa cagaacactt aagatcacgc
     3601 aaagagtggg tgaaattaga ccgcttgcga gtctgttttt atgcaaaagc ggttgaggga
     3661 gaaataaata acaactggag aagtcgtgat ttagacgagc tggttaacca atggtcttgg
     3721 tcagaaaaac aaattcaatt acttcaaaac cgtcaacatt ggaaaattaa gcaagtgatg
     3781 aatcatgaga atatgctgat tgatcattta ctacaaagtt atcgaaattt aattcatttt
     3841 gctcgaaaat ttcatattaa tccgagcatt atgacaaatg atactgatat tttaatgcga
     3901 aaactttatt ctgtatttga agcacttcca ggaaaaatat ctttaattaa tcataaaata
     3961 tcgtccaatt tagcagaaaa agcaattaca tttattgaag ctaaagttgg atctgcgtta
     4021 caacccggtt ggtatttagt taatcaggca cctaaaagtc gttatgactc tgaaaatcgt
     4081 tatgtccatt ataataaaag cccgattaag cttattgctt gggggtattt taacggcatt
     4141 attaacgcag caactaattt acatactgtt agccctaatt tagatatcga gaaattgcga
     4201 caatttatca ccgacttgcg cttatctttc ccaattcaag cgccggaagt gaccaatgat
     4261 gacatgcatc accctaatga aattcgcaat ttggttatag cgcttaattt agtacaagat
     4321 ccaaccgaac atatcgatga actaccaaaa aaagtttatc caagcgatct gtttaatttt
     4381 ggtaaggcac aaaaaacgtt agttggtagt gtgagcatta tttatcgaaa tgtatggaat
     4441 gaaattcgaa ctcaacattt tgacggtgaa gatgctattt taaaagcact aaaattggta
     4501 tccaatcgaa tttatcaagg aaattcttca cctcaatcag ttaatgtttt ttgctatagt
     4561 aaatatctac gtagtgagtt acgtgatttt gtggcagatt tagttcataa atgtattaca
     4621 attcagactg gttgtttact gcaagagaat gaatttagca ctttaaaagt agcaggaaaa
     4681 acctggcagt ttctttttca taaaaaaaca tttagcatta aacaaattga tgaacaacaa
     4741 gcggttgaaa atttaaattc ttttgcaaaa accaagcaaa atgctaaaag cgaaaatgaa
     4801 aagaaaaaag aatttccgca gcaaattagt gaatttgcca gtgaaggctt tttacagttt
     4861 ttctttgaag acaatgtcga taatagtttt aatgtatatg ttgtagatga acgtaataat
     4921 gtcgaaagtt actataactg tttcggcaat aaagaagaga aaatcaggaa aatcaataaa
     4981 ttacaaaat
//
LOCUS       DANLRK010000172         4946 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.172, whole genome shotgun sequence.
ACCESSION   DANLRK010000172 DANLRK010000000
VERSION     DANLRK010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4946
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.172"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            <1..583
                     /locus_tag="PXA33_002222"
     CDS             <1..583
                     /locus_tag="PXA33_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDL5121522.1"
                     /translation="YTTIMQRISGAKQIDSLTVKIKDDIESQTAEKSIAELLVAKHGK
                     KDFFIMNSDTIKQTITSTTNTMTLLISSIALISLIVGGIGVMNIMLVSVTERTREIGV
                     RMAIGAKQRNILQQFLIEAVVICLIGGVIGILLASAIIWAFNTLGTNFKMMLSPESVI
                     IAVLCSTLIGVVFGYIPARNAAQLNPITALAQE"
     gene            667..1437
                     /locus_tag="PXA33_002223"
     CDS             667..1437
                     /locus_tag="PXA33_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HDL5121523.1"
                     /translation="MTITNTELEQILNTKLNSSAINDYAPNGLQVEGKREIKKIITGV
                     TATLPLIEKAIEKNADAILVHHGYFWKSENPCIRGMKGKRIKQLLVNDINLFGYHLPL
                     DIHSELGNNAQLAKHLGVKNLQGLEDRPNSIPMYGELENPISANELKQKLEKTLQRTV
                     ILCDEFVHSPQKMIKTIGICSGGGQGYIDLAFEKGCDAFISGEISEQTTHSAREQGIY
                     YFACGHHATERDGVKALGEWLAVEYGLDVEFIDIDNPA"
     gene            complement(1518..2648)
                     /gene="nrdB"
                     /locus_tag="PXA33_002224"
     CDS             complement(1518..2648)
                     /gene="nrdB"
                     /locus_tag="PXA33_002224"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HDL5121524.1"
                     /translation="MAYTTFSQHKNDQLKEPMFFGQNVNVARYDQQKYEIFEKLIEKQ
                     LSFFWRPEEVDVSQDRIDYAQLPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLVSI
                     PELETWIETWTFSETIHSRSYTHIIRNIVNDPSIVFDDIVTNEEIIKRAKDISAYYDD
                     LIRDSQLYSLYGEGSYQVDGKTCEVSLRSLKRQLYLCMMSVNALEAIRFYVSFACSFA
                     FAERQLMEGNAKIIKFIARDEALHLTGTQHILNIMAYGNDDPEMAEIARECRQEAYEL
                     FLSAAEQEKAWAEYLFKDGSMIGLNRDILVQYIEYITNIRMQAVGLPLPFKARSNPIP
                     WINAWLVSDNVQVAPQEVEVSSYLVGQIDAKVDSRDFGDFEL"
     gene            complement(2675..4945)
                     /gene="nrdA"
                     /locus_tag="PXA33_002225"
     CDS             complement(2675..4945)
                     /gene="nrdA"
                     /locus_tag="PXA33_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018356743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HDL5121525.1"
                     /translation="MNKSLMVTKRNGTREQLNLDKIHRVITWAAEGLENVSVSQVELR
                     SHIQFYEGIRTSDIHETIIKAAADLITKDTPDYQYLAARLAIFHLRKKAYGQFEPPHL
                     YEHVKKLVALGKYDADLLSDYTPEEWETMNGFIDHWRDMTFSYAAVKQLEGKYLVQNR
                     VSGEIYESAQFLYLLVAACLFSKYPTASRLDYIRRFYDATSTFKISLPTPIMAGVRTP
                     TRQFSSCVLIECDDSLDSINATSAAIVKYVSQRAGIGINAGAIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATVYFPIWHLEAESLMVLKNNRGVEENRARHMD
                     YGVQINKMMYQRLIKGGEISLFSPSDVPGLYEAFFADQAKFEQLYLQYEQDPSIRQRK
                     VKAVELFSLLMQERASTGRIYIQNVDHCNTHSPFNPRVAPIRQSNLCLEIALPTKPLA
                     HFYDEQGEIALCTLSAFNLGTLSNLDELEELADLVVRALDALLDYQDYPVLAARNSSL
                     NRRALGVGVINYAYYLAKNQVRYSDGSANNLTHRTFEAIQYYLLKASMNLAKEQGACA
                     YFDQTTYAKGLLPIDTYKKEIDELTQEPLHYDWEQLRQDILQFGLRNSTLTALMPSET
                     SSQISNATNGIEPPRGYISVKASKDGILKQVVPEYERLKAYYELLWDIPNMDGYLHLV
                     GIMQKFVDQAISANTNYDPNRFAEAKVPMKVLLKDLLTAYKYGLKTLYYQNTRDGAED
                     VQDDLDEGCAGGACKI"
BASE COUNT         1529 a         1040 c          916 g         1461 t
ORIGIN      
        1 ttataccact attatgcaac gcatttcagg tgcaaaacag atagattcac tcacggttaa
       61 aattaaagat gatattgaaa gccaaactgc cgaaaaaagc attgccgaat tgttagtagc
      121 taaacacggc aaaaaagatt tctttattat gaatagtgat acgatcaaac aaaccattac
      181 cagtaccacc aatacaatga ctttgctgat ctcctctatc gcccttattt cgctgattgt
      241 cggtggtatc ggggtaatga atattatgct cgtttccgtc accgaacgca cccgagaaat
      301 tggtgtccga atggcgattg gcgcaaaaca acgcaatatt ttgcaacaat ttttaattga
      361 ggcggtggtt atttgcctaa taggtggtgt gattggcatt ttactggcaa gcgctattat
      421 ttgggcattt aataccttag gtacgaattt taaaatgatg ctttcaccgg aatcggtgat
      481 tattgccgtg ctctgctcta cacttatcgg tgtggtattt ggctacattc cggcaagaaa
      541 cgccgcccaa ttaaacccaa ttactgctct ggcacaagag taaatgcgat aactatctgc
      601 tacactgtag gggcaaaaaa ttttttgccc ctacatccat tattatgcca agtttaagga
      661 gataaaatga caataaccaa tactgaacta gaacaaattc tgaacaccaa actcaacagc
      721 tccgccatta acgactatgc cccaaatggc ttgcaagttg aagggaaaag agagatcaaa
      781 aaaattatta ccggtgtgac agctacactt cccctgattg aaaaagcgat tgagaaaaac
      841 gccgatgcaa ttttagtcca ccacggttat ttttggaaaa gtgaaaaccc gtgcattcga
      901 ggaatgaaag gtaaacgcat taaacagctt ttagtgaatg acatcaatct ctttggttat
      961 cacctaccac tggatattca ctccgaacta ggtaacaacg cccagcttgc taagcattta
     1021 ggagtaaaaa atctacaagg tttagaagac agacctaatt caattccaat gtacggtgaa
     1081 ttagaaaatc cgatttccgc caatgaactg aaacaaaaac ttgaaaaaac gttacaacgc
     1141 accgttattt tatgtgatga atttgttcat agcccacaaa aaatgattaa aacaataggg
     1201 atttgctccg gcggaggaca aggctatatt gatttagcct ttgaaaaagg ctgtgatgcc
     1261 tttattagtg gcgaaatttc cgaacagacc actcactctg cccgtgaaca aggaatctat
     1321 tattttgctt gcggtcatca cgccacggaa cgagatggcg taaaagcctt aggtgaatgg
     1381 ttagccgttg aatatggttt agatgttgaa tttattgata ttgataaccc tgcataaaaa
     1441 atacaagcgg ttgaatttcc tgaaaatttt gcaatttgca aaaaatcact aaaaatcaac
     1501 cgcttgtata tcatagtcta aagttcaaaa tcgccaaaat cacggctatc tactttagca
     1561 tcaatctggc caaccaaata agaactgact tccacttctt gtggtgctac ctgtacatta
     1621 tcggaaacca accacgcatt gatccacgga atggggttgg aacgtgcctt aaatggtaat
     1681 ggtagtccaa cagcctgcat ccgaatatta gtgatatatt caatatattg caccaaaata
     1741 tcccgattta gccctatcat cgaaccgtct ttaaacaggt attccgccca cgctttttct
     1801 tgttcagctg cacttaaaaa aagctcgtag gcctcttggc gacattcacg tgcaatttct
     1861 gccatttcag gatcatcatt gccataggcc ataatattca atatatgctg agtgccggta
     1921 agatgtaatg cttcatcacg tgcaataaat ttgatgattt tggcatttcc ttccataagc
     1981 tgccgttccg caaaggcaaa tgagcaagca aaagagacat aaaaacgaat tgcttcaagc
     2041 gcattcacac tcatcataca gagataaagt tgtcttttta gactacgcaa actgacctca
     2101 caggtttttc catcaacctg ataactgcct tcaccataca aactatagag ttggctgtca
     2161 cgaatcaaat catcataata ggcggaaata tctttggcac gcttaataat ttcttcgttt
     2221 gtgacaatat catcaaatac tattgatgga tcattgacaa tattacggat gatatgtgtg
     2281 taagaacgac tgtgaattgt ttcgctaaat gtccaagttt caatccaggt ttcaagctcg
     2341 gggatagaca ctaatggcaa aagcgccaca ttcgggctac gcccttgaat ggaatccaac
     2401 aaggtttggt attttaaatt gctgataaaa atgtgctttt catgttcagg cagttgggca
     2461 tagtcaatgc ggtcttgtga cacatccact tcttccggtc gccagaaaaa tgagagttgt
     2521 ttttcaatca gtttttcaaa aatttcgtat ttttgttgat cataacgcgc cacattcaca
     2581 ttttgtccaa aaaacatggg ttcttttagt tgatcatttt tatgttgtga aaacgtagta
     2641 tatgccatgt tattctcctt tatttctgtt cttattaaat tttacaagca ccaccagcac
     2701 agccttcatc taagtcgtct tgcacatctt ctgctccatc acgggtattt tgatagtaaa
     2761 gggtttttaa cccgtattta tacgccgtta atagatcttt taataatact ttcatcggta
     2821 ctttagcttc tgcaaagcgg tttgggtcgt aatttgtatt cgccgaaatc gcttggtcaa
     2881 caaatttctg cataatccca accaaatgca aatatccgtc catattagga atatcccaaa
     2941 gcagttcata gtatgctttt aaacgttcat actccggcac aacctgcttt aaaataccgt
     3001 cttttgatgc tttaacacta atatagccac gcggaggttc aattccattg gtggcattcg
     3061 aaatttgcga cgaagtctcc gatggcatta aggcagttaa agtggaattg cgcaagccaa
     3121 attgcagaat atcctgacgt agttgttccc aatcataatg aagcggttct tgagttaatt
     3181 catcaatttc ttttttataa gtatcgatag ggagtaaccc tttggcataa gtggtttgat
     3241 caaaataagc acacgcccct tgttctttgg ctaaattcat cgatgctttt aataaataat
     3301 actgaatcgc ttcaaaggta cggtgagtta aattattagc actgccatcg gaataacgca
     3361 cttggttttt agctaaatag taagcatagt taatcacgcc tacacctaat gcgcgacgat
     3421 ttaatgagct gttacgtgca gctaataccg gataatcttg ataatctaat agggcatcta
     3481 gcgcacgaac cactaaatca gccaattctt ctaactcatc taaattgctg agtgtgccta
     3541 aattaaatgc cgataatgta cataatgcga tttccccttg ttcatcataa aaatgtgcca
     3601 gtggcttagt tggcaaagca atttctaagc agaggttcga ttggcgtatc ggtgcgacac
     3661 gaggattaaa tgggctatga gtattacaat gatcgacatt ctgaatataa attcgccctg
     3721 ttgaagcccg ctcttgcatc agtaatgaaa acaattctac agctttaact ttacgttgcc
     3781 gaatgctcgg atcttgctca tattgcaaat aaagttgctc aaatttagct tgatcagcaa
     3841 aaaacgcttc atataacccc ggcacatctg atggactaaa taaactaatc tcaccgcctt
     3901 taattaaacg ttgatacatc attttattaa tctgtacccc gtaatccata tggcgagcac
     3961 ggttttcttc cacaccacgg ttatttttta ataccattaa gctttctgct tccaaatgcc
     4021 aaatcggaaa atacacggta gccgctccac cgcgaacacc accttgagag caagacttta
     4081 ctgcagtttg aaaatgttta taaaagggaa tacagccggt atgaaatgct tctccaccac
     4141 gaatggggct acctaatgcc cgaattgcgc cggcattaat accgatccca gcacgctgtg
     4201 aaacatattt aacgattgca gccgaggttg cattgatgga atctaagcta tcatcacact
     4261 caattaacac acaggaacta aattgacgag taggtgtacg cactcctgcc ataatcggtg
     4321 ttggcaaaga aattttgaaa gtggaagtcg catcataaaa acggcgaata taatctaagc
     4381 ggcttgctgt tggatactta gaaaataaac aggcagctac gagtaaatac aaaaattgag
     4441 ccgattcata aatctctccg ctaacacgat tctgcactaa atattttcct tccaactgct
     4501 ttactgctgc ataggaaaac gtcatatccc gccagtgatc aataaatcca ttcattgtct
     4561 cccattcttc gggcgtatag tcgctcagca agtctgcatc atatttacct agcgcaacta
     4621 attttttaac gtgctcatat aaatgcggtg gctcaaattg tccataagct tttttgcgta
     4681 agtggaaaat cgccaagcgt gcggctaaat attgataatc tggcgtatcc ttagtgatta
     4741 gatcagctgc agccttgata atagtttcat gaatatctga agtgcgaata ccttcataaa
     4801 attgaatgtg agaacggagt tctacttgtg aaacagaaac attctctaac ccttctgccg
     4861 cccaagtaat cacacgatga attttatcta gattgagttg ctcgcgcgtg ccattacgct
     4921 ttgttaccat taaactttta ttcata
//
LOCUS       DANLRK010000173         4906 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.173, whole genome shotgun sequence.
ACCESSION   DANLRK010000173 DANLRK010000000
VERSION     DANLRK010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4906
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.173"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            46..375
                     /gene="trxA"
                     /locus_tag="PXA33_002226"
     CDS             46..375
                     /gene="trxA"
                     /locus_tag="PXA33_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018357021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HDL5121526.1"
                     /translation="MSNVIHTTDATFEQDVLKSDVPVLLDFWAPWCGPCRMIGPVLDD
                     LSVDAQFDGKVKIVKMNVDENPSVPAQFGVRSIPFLLLFKNGEVIAQQVGAIPHAQMA
                     EFIKQAL"
     gene            546..1769
                     /gene="hflK"
                     /locus_tag="PXA33_002227"
     CDS             546..1769
                     /gene="hflK"
                     /locus_tag="PXA33_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HDL5121527.1"
                     /translation="MSWNESGNQQDPWGKPGQKKAEPKPEQQEQGSQQEPTNNQRNEQ
                     QPPDLEEVFSSLLKKMGGGKGPNNSSNQPSASLGKFLPVVLGLAAVVWVGSGFYTVQE
                     AERGVVTRLGKLNDIVLPGLNWKPTFIDSVTRVNVERVSELNTSGSMLTQDENMVQVE
                     MTVQYRVEDPAKYLFSVSNPDDSLKQATDSALRYVIGHMTMDEILTTGRATVRERTWS
                     TLRDIIKTYDMGLLVTDVNFQYARPPEEVKAAFDDAIKAQEDEQRLIREAEAYARGEE
                     PIARGQAQRTIEQAQAYKEAVVLNAKGEVERLSQLLPEYKASPELTRERLYIQTMEKV
                     MKNTPKVVMDSSGNNLNVLPFDKLMNSSSVIKAEQPAAAQVVTPQVVQPQVQPRTQIQ
                     PQQAEQQEPTRKGRF"
     gene            1772..2659
                     /gene="hflC"
                     /locus_tag="PXA33_002228"
     CDS             1772..2659
                     /gene="hflC"
                     /locus_tag="PXA33_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HDL5121528.1"
                     /translation="MRRLLVPILAVVAFVVLQAITIVNEGERGIMLRFNKVHRDSDQK
                     VVVYEPGIHFKVPFIDSLKVLDARIQTLDGQEDRFVTVEKKDLLVDSYVKWRISDFGQ
                     FYTSTGGDYQKAADLLRRKVGDRLRSEIGSRTIKDIVSGSRGELMAGAQKALNAGEDG
                     AERLGIEVVDVRVKQINLPNEVSSSIYQRMRAERDAVAREHRSQGNEKAEVIRAEVDK
                     KVVLILANANKTAQALRGEGDAQAAKLYSEKFGNEPEFYSFIRSLKAYEDSFAEGQNN
                     MMLLKPNSEFLRFMQAPTK"
     gene            2833..4131
                     /gene="purA"
                     /locus_tag="PXA33_002229"
     CDS             2833..4131
                     /gene="purA"
                     /locus_tag="PXA33_002229"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010869263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HDL5121529.1"
                     /translation="MGKSVAILGAQWGDEGKGKIVDLLTDRVKYVVRYQGGHNAGHTL
                     IINGEKTVLRLIPSGILRDNVTCLIGNGVVLSPEALMKEMGELEERGINVRERLKISE
                     ACPLILPYHVAMDHAREAALGKNKIGTTGRGIGPAYEDKVARRGLRVSDLFDKEHFAA
                     KLKDILDYYNFQLVNYYKVEAVDYQKTLDDVFAIADVITGMVADVTTLLHEARENGDN
                     ILFEGAQGTMLDIDHGTYPFVTSSNTTAGGVATGSGYGPRHLDYVLGIIKAYCTRVGS
                     GPFTTELFDDVGAEIARKGNEFGAVTGRPRRCGWFDAVAVRRAVQINSISGFCMTKLD
                     VLDGFKELKICVAYKMPNGKIVEYAPLAAKDWEGVEPIYETMPGWSENTFRVTKREDL
                     PQAALDYIKRIEELVGVPVDILSTGPDRVETMILRDPFAA"
     gene            complement(4291..4361)
                     /locus_tag="PXA33_002230"
     tRNA            complement(4291..4361)
                     /locus_tag="PXA33_002230"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4331..4333),aa:Arg,seq:tct)
     gene            complement(4510..4803)
                     /locus_tag="PXA33_002231"
     CDS             complement(4510..4803)
                     /locus_tag="PXA33_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121530.1"
                     /translation="MTTFNKILNPMYSTIASYSTQDDGSLNAKYVVGTGDDTDGEVTN
                     FVIITSEYKYIDAQSAKEITDAPLTKEDIGKTPTQIMLGRIYKYLKETGQIVV"
BASE COUNT         1500 a          873 c         1123 g         1410 t
ORIGIN      
        1 cagttagggc gtttcttttt ttatttaata tttaaggata aacttatgag caatgtcatt
       61 catacaacag atgctacatt tgaacaagat gttttaaaat cagatgtacc ggtattatta
      121 gacttctggg cgccttggtg tggtccttgc cgtatgatcg gccccgtact tgacgattta
      181 tctgttgatg cacaatttga tggtaaagta aaaattgtca aaatgaacgt agatgaaaat
      241 ccatctgtac cagcacaatt tggcgtacgc agcattccat ttttactatt attcaaaaat
      301 ggtgaagtga ttgcccagca agttggtgca attccgcacg cccaaatggc tgaatttatc
      361 aaacaagcat tataatttta agagcaatac tagggcgaat ttatcgccct atttttttgc
      421 aaataaatag tgaaaaaaga ccgcttgttt cagataaacc tagtcaaaag cttactaaat
      481 aggtacaatc actaacttgc attctaagct ttaaagtgcg attcgatttt attttggaga
      541 tatttatgtc gtggaatgaa tcaggcaatc agcaagatcc ttggggtaaa ccggggcaga
      601 aaaaagctga gccaaagcct gagcaacaag aacagggttc acaacaagag ccgaccaata
      661 atcaaagaaa tgaacaacag ccccctgatt tagaagaagt atttagcagt ttattaaaaa
      721 aaatgggggg tggaaaaggg cctaataata gttcaaatca acctagtgca agcttaggga
      781 aatttctacc tgtggtttta ggtcttgcag cagtagtttg ggttggctca ggtttttaca
      841 ctgtgcaaga ggctgaacgt ggtgtggtta ctcgtttagg taagctgaat gatatcgtgt
      901 tgccagggtt gaattggaaa ccgactttta ttgattcggt tacaagagta aacgtagaac
      961 gtgtttcaga gctgaacacc agtggctcaa tgctgactca agatgaaaat atggtgcagg
     1021 tagaaatgac ggttcagtat cgtgtggaag acccggctaa atatctgttt agtgtgagca
     1081 accctgatga cagcttaaaa caagcaacag acagtgcctt gcgttatgtg attggtcata
     1141 tgacgatgga tgaaatttta accacagggc gtgcaaccgt gcgtgaaaga acgtggtcga
     1201 cattgcgtga tattatcaaa acctatgata tgggtttatt ggtaacagac gtaaacttcc
     1261 aatatgcacg accgcctgaa gaggtgaaag ccgcctttga tgatgcaatc aaagcacaag
     1321 aagatgagca acgcttaatt cgtgaagcgg aagcctatgc aagaggtgaa gagccgattg
     1381 cacgtggcca ggcacagcgt acgattgagc aagctcaagc ttacaaagaa gcagttgtgt
     1441 taaatgcgaa aggggaagtg gagcgtttga gtcaattatt acctgaatat aaagcctctc
     1501 cagagctgac tcgtgaacgt ttatatatcc agacaatgga aaaagtcatg aaaaatacgc
     1561 cgaaagtggt gatggattca agtggtaata atttgaatgt attgcctttc gataagctaa
     1621 tgaattcttc atcggtaata aaagcggagc agcctgcggc ggcacaagta gttacaccac
     1681 aagttgttca gccgcaagta cagccaagaa cacaaattca gccgcaacaa gcagaacaac
     1741 aagaaccaac tcgtaagggg agattttaat catgcgtaga ttattagtac ctatacttgc
     1801 tgtagttgct tttgttgtgc tacaggcaat caccattgta aacgagggcg agcgtggcat
     1861 tatgttacgt tttaacaaag ttcatcgtga tagcgatcaa aaagtggtgg tatatgaacc
     1921 ggggattcac tttaaagtac catttatcga tagtttaaag gtattagatg ctcgtattca
     1981 aactctagat gggcaagaag accgttttgt aacagtcgag aaaaaagatt tattagtgga
     2041 ctcctatgta aaatggcgaa tcagtgattt tggccaattt tatacctcta ctggggggga
     2101 ttaccaaaaa gcggcggatt tattacgccg taaggtaggg gatcgtctgc gttccgagat
     2161 tggttctcga accattaaag atattgtatc cggctctcgt ggtgagttaa tggcaggagc
     2221 ccaaaaagcg ctgaatgccg gtgaagatgg agcagagcgt ttaggaattg aagtggttga
     2281 tgtgcgagtg aagcaaatca acttaccaaa tgaagtttct tcttcaattt accaacgtat
     2341 gcgtgccgag cgtgatgcgg ttgctcgtga acaccgctca caaggtaatg agaaagcaga
     2401 ggtaattcgt gccgaagtgg ataaaaaagt ggtgctgatt ttggcaaatg cgaacaaaac
     2461 tgcccaagcc ttgcgaggtg aaggtgacgc tcaagctgca aaactctatt cagagaaatt
     2521 tggtaacgag ccggaatttt acagttttat tcgtagttta aaagcctatg aagatagctt
     2581 tgccgaaggt caaaataata tgatgttatt aaagccaaac agcgaattct tacgctttat
     2641 gcaagcaccg acaaaataat tgtatctata aaacccgatc ttgtatcggg tttttaattt
     2701 tcatccctat tctgatcata acaagcggta aattttggct gattttttgc aaaaaatttg
     2761 aaaaaattga ccgcttgcga tatgaagtta taatgggttt cgttattcat cctaactaaa
     2821 agaggcattt ctatgggtaa aagtgttgct attcttggcg ctcagtgggg cgatgaggga
     2881 aaaggcaaaa ttgttgattt attaacagat cgtgtgaaat atgtagtacg ttatcaaggt
     2941 ggacataatg cagggcatac cttaatcatt aatggtgaaa aaaccgttct gcgtttaatt
     3001 ccatcgggta ttttgcgtga taacgtgact tgtttaatcg gtaatggtgt agtgctttct
     3061 ccagaggcat taatgaaaga gatgggggag ctagaagaac gaggcattaa tgtgcgtgag
     3121 cgtttaaaaa tttctgaagc ttgcccgtta attctgcctt atcacgttgc gatggatcac
     3181 gctcgagaag cggcacttgg taaaaacaaa attggtacaa caggacgtgg tattggccca
     3241 gcttatgaag ataaagtcgc tcgccgtggt ttacgtgtca gcgatttatt cgacaaagaa
     3301 cattttgccg caaaattaaa agatatttta gattactaca acttccaatt agtcaattat
     3361 tacaaagttg aagcggtaga ttatcaaaaa accttagatg atgtgtttgc gattgcggat
     3421 gtgattaccg gaatggttgc cgatgtgaca actttacttc acgaagcacg tgaaaacggc
     3481 gacaatattt tatttgaagg ggctcaaggc acgatgttag atatcgacca cggtacttac
     3541 ccgttcgtaa ccagctcgaa tacgacagca ggcggtgtgg caaccggatc aggctacggt
     3601 cctcgtcatt tagattatgt attagggatt atcaaagcct actgtacccg tgtaggtagt
     3661 ggaccgttca ccaccgaact atttgacgat gtaggtgctg aaattgctcg caaaggtaat
     3721 gagttcggag cggtaaccgg tcgccctcgc cgttgtggct ggtttgatgc cgtagccgtt
     3781 cgccgagcag tgcaaatcaa ctccatttca ggcttctgta tgactaaact cgatgtttta
     3841 gacggattca aagagctaaa aatctgcgtt gcctacaaaa tgccaaacgg aaaaattgtc
     3901 gaatatgccc ctcttgcagc aaaagactgg gaaggtgttg aaccaattta tgaaacaatg
     3961 ccgggttggt ctgaaaatac tttccgtgtg acaaaacgtg aagatttacc acaagccgca
     4021 ttagattaca ttaaacgtat cgaagagctg gtcggtgtgc cggtagatat tctttctaca
     4081 ggtccggatc gtgtcgaaac gatgattttg cgtgatcctt ttgcagctta ataaaagata
     4141 aaataaagcc atttagtgag atagagctag atggcttttt tgtttgaacc agtaaacttt
     4201 actttggcac aattttgtca caatacagaa aatagttctt tagcgtgagt ttttgaggct
     4261 gtgtaagtat ttgaatttaa aagaatttaa tggcacgccc taaaggattc gaacctttga
     4321 cccacgcctt agaagggcga taggtatttt ataaaaggcg tttattttaa ctaataaaaa
     4381 caaagactta tagagataac ccctctatat tacctaccat tatttcacat ctttttacat
     4441 taaataacac catttacggt acatttatgg aacacgttgg tgatgagcta gtcttcaaaa
     4501 gttactaaat taaactacaa tctgccccgt ctcttttaag tacttataga tacgccctaa
     4561 cataatctgc gttggcgttt tgccaatatc ctcttttgtt agcggtgcat cagtaatctc
     4621 cttggcggac tgtgcatcaa tgtatttgta ctcacttgtg atgattacaa agtttgttac
     4681 ttctccatcg gtatcatcgc ctgtaccgac cacatattta gcattgagcg agccgtcatc
     4741 ttgcgttgag tagcttgcga tggtggaata cattgggttt aagattttgt taaaagttgt
     4801 cataattttt ccttttgtgg attgttgata aaaaaagacc gcttgtagtc atacaaacgg
     4861 cttagtagga agagtaaatt gctttacata atataacctt ttattt
//
LOCUS       DANLRK010000174         4863 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.174, whole genome shotgun sequence.
ACCESSION   DANLRK010000174 DANLRK010000000
VERSION     DANLRK010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4863
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.174"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(69..635)
                     /locus_tag="PXA33_002232"
     CDS             complement(69..635)
                     /locus_tag="PXA33_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121531.1"
                     /translation="MEGLIMGLQLIPHFPELAISRRTYLHDMPMYCFVSKNWIFSAKD
                     RAKREALGNLLACGGEMMKSFIFKRSLFSEIWFEDILLEDEEKELIRILYNKPEPEYQ
                     VEEKIDWEAREARRKQADYDKWQSYDERNPFHYQIFPTDTIAYAQQQVEKFIEDVDNF
                     LEWFYDDEVKRYILERNEIYQKKFDLVN"
     gene            complement(1168..1494)
                     /locus_tag="PXA33_002233"
     CDS             complement(1168..1494)
                     /locus_tag="PXA33_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL5121532.1"
                     /translation="MLHSKGKILPKITLNEIETKERFAIALRKEMHAQYWTGKMVANA
                     LDVSEKTVKDWVAGKRLPSSLDLLALMRISSCVRQLVWDISKSEECYQDAEMLKQICF
                     VLLSNL"
     gene            1542..3173
                     /locus_tag="PXA33_002234"
     CDS             1542..3173
                     /locus_tag="PXA33_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL5121533.1"
                     /translation="MVTRTKNQPKNQAVSYIRMSTEHQEFSPDLQRCFIENYAKENGL
                     EIIREYIDEGKSGLTAEHRPQFLSMIQLVQSGQADFDHILVYDVSRWGRFMNIDESGH
                     YELICTYQGIKVHYCAEPFKGNDIASQIYKTMKRLSVGDYCRELGVKVFNGQKNLIQH
                     GFRQGGVAGFGLRRRLIDCQGNPKFDLQRGDRKSLQTDRVILVAGPKEEQEIVRQIYH
                     DFVYQHKTEQQIADSLNAQGIVTDRNTAWTKGVVHQILINEKYVGHNVWNKRSASKTR
                     EYKGKNPVEEWVRAENVFEAIVPQALFNAAQTIIHQRSARLSDDEMLEQLKLLLKEKG
                     KLSGLIIDEAENCPSSSAYSSRFGSLLNTYTLINYEPERDYHYVEINRLLRQQHKNFV
                     QQTLDTIIELGGVVLSDDVTDLVHINNEFTASIVLSRCRLTPSGSKRWLIRFDTSLNP
                     DITIAVRINESATEILDYYLLPTTEKVNEKLRLAESNPAELEIYRHDNLDRFFIMVER
                     ILVKEFIYAKRHHSNSSYRQNSGTQSQNAQSESLC"
     gene            3088..3981
                     /locus_tag="PXA33_002235"
     CDS             3088..3981
                     /locus_tag="PXA33_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HDL5121534.1"
                     /translation="MQNDTIQILPIDKIRVLNPRTRNQKVFADLVENIATIGLKRPIT
                     VTPSVDSAEYYDLLCGQGRLEACQVLGELFIPCRVVNANSEESYLISLVENIARRQHS
                     YMELLSGIKILNDRGYKPVDIARKIGFSKSHVNSILHLINAGEERLINAVEQGILPIT
                     IAISISRSDDQEVQKQLAELYTNGTLKSTDIAKIRNLIHRRNLAGKKANTGISGSIRS
                     SIYNPKTVVNIYKEETERQKMMIKQADYDEKQLSIILSCLNKLFEDKYFQLVLKSEHL
                     DDMPKDLSQRMLKQREGNIYA"
     gene            3974..4837
                     /locus_tag="PXA33_002236"
     CDS             3974..4837
                     /locus_tag="PXA33_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HDL5121535.1"
                     /translation="MPKHITYAFHNQLISLPIDEIIPLKKMDKNFEKSTKFQSILASI
                     REIGIVEPVVVYPEENNQYILLDGHSRVHALKKLGVDKVLCMISTDDEGFTYNKQINR
                     LTAVQENKMLMKTIERGVSDELIARTLNINLKALRQKMNMLNGITREVVDILANKQVS
                     KEIFRILRKMKPERQIEVAQMMIASNKFTLTYANMMLLSSRKEELVESHKSKTTNDDL
                     TDLSIMQKELERLKENYKISEEKLADLKMGLVVAKGYVKKLLNNAAVVDLLENDQSEI
                     YLTLKEVCDIQ"
BASE COUNT         1608 a          928 c          938 g         1389 t
ORIGIN      
        1 acaaatatca agtgtaaaaa aatataaaag tcggcaataa cattatttta taataactaa
       61 tgtgaattct agttcactaa atcgaatttc ttttggtaaa tctcattcct ttctaatata
      121 tagcgtttta cctcatcatc gtaaaaccat tcaagaaagt tatcaacatc ttcaatgaac
      181 ttttctacct gttgctgtgc atatgctatc gtatcggtag ggaagatttg ataatgaaac
      241 ggattgcgtt catcataact ctgccatttg tcataatcgg cttgtttacg ccgcgcttct
      301 cttgcctccc agtcgatttt ttcttccact tgatattctg gctctggctt attatacaaa
      361 atacggatga gttccttttc ttcatcctca agcagaatat cttcaaacca gatttcagaa
      421 aataggctgc gcttgaaaat gaaagacttc atcatttccc ctccacacgc caataagttt
      481 cccaatgctt cccgtttcgc tctatctttt gccgaaaata tccagttttt cgatacaaaa
      541 cagtacattg gcatgtcatg caaataggtt cttcgtgaaa tggctaattc aggaaaatga
      601 ggaataagct gtaaccccat tattaagcct tccatactgc acacgatctt acttaattgt
      661 ccgtaatcgc gtaaaaaagc aatgtcagtt tttatcaacc acaacgatag gctgactaat
      721 atgaacataa agatgagata atagatcggt tttaaggcta aggcgcgccg acaaagccac
      781 attaagagtg gaatcgtaac gagatcaaag aaaaatgatg agtctatgat gatttcctta
      841 tgtgagttga tgaataatca ggcatgaagg aaaccttaaa aaggaaaagt cccttcatta
      901 ccacttttta aaacctttta aggcttaaaa atcaaaagtc gtaatgaagg gactattgtc
      961 cgtgggcaaa aaggtattag ttataggggg ctttgtcaag cgaaaattta ctgtgcattt
     1021 tctcgccaaa actgatttaa gccttgataa aatctcgaaa gtaccttgtc aagaaaaatc
     1081 tttttagtgg tttgattgct agttttttga gcaaaactat gctgtaatgc agtaaaaata
     1141 cagtttctat ctgtgtttgg aatccagtta caaattggag agtaatacaa agcatatttg
     1201 cttgagcatc tcagcatctt ggtagcattc ttctgatttt gaaatatccc aaacaagctg
     1261 ccgtacacat gatgaaattc tcattaacgc caataaatct aggctacttg gcaatctttt
     1321 ccctgcaacc caatctttaa ctgttttctc actcacatct aatgcattgg cgaccatttt
     1381 ccccgtccaa tattgagcat gcatttcctt tcgtaacgca attgcaaacc gctctttcgt
     1441 ttcaatctca ttgagtgtaa ttttgggtaa gattttaccc tttgagtgca acatatttcc
     1501 tcccgattcc tttatattgg tgtagataag gagtcattag aatggttaca cgtacaaaga
     1561 atcaacctaa aaatcaggcg gtttcctaca ttcgaatgtc aacggaacac caagaatttt
     1621 caccagacct tcaacgttgc tttatcgaaa attatgctaa agaaaatggg ctagaaatca
     1681 ttagagaata cattgatgaa ggaaaaagtg ggctaaccgc agaacaccgc ccacaattcc
     1741 tttcaatgat acaactcgta caaagtggac aagccgactt cgatcatatt ttggtgtatg
     1801 acgtgagtcg ttggggtcgg tttatgaata ttgatgaatc ggggcattat gaactgattt
     1861 gtacctacca aggcattaaa gtccactatt gcgccgagcc attcaaaggg aatgatattg
     1921 cctctcaaat atataaaacc atgaaacgtt tgtcggtagg agactactgt cgtgaactcg
     1981 gagtgaaagt atttaacggt caaaaaaatc tgatccaaca tggatttaga caaggtgggg
     2041 tagctggatt tggattgcgt aggagattga ttgactgtca aggaaaccct aaatttgatc
     2101 tccaacgtgg agatagaaag agcctacaaa ccgacagagt gattttggtt gcaggcccaa
     2161 aggaagagca ggaaattgtc cgacaaattt accatgattt tgtttatcaa cataaaacgg
     2221 aacaacaaat tgctgatagc ttaaatgcac aaggcattgt tactgataga aatacagcat
     2281 ggacgaaagg cgtagtacat caaatcctta ttaacgaaaa gtatgtcggc cacaatgttt
     2341 ggaataaacg tagtgcatca aaaacacgcg aatataaagg taaaaacccc gtagaagagt
     2401 gggtgcgagc tgagaatgta tttgaagcta tcgtgccaca agccttattt aatgcagccc
     2461 aaaccattat tcatcaacgc tccgcccgat tatctgacga tgaaatgtta gagcaactca
     2521 agctactttt gaaagagaaa gggaagctgt ctggcttgat cattgatgaa gccgaaaatt
     2581 gtccatcaag tagcgcgtac agtagccgat ttggaagcct acttaacaca tatactttaa
     2641 ttaactatga acccgaacgg gattaccact atgtagagat caaccgatta ctccgtcagc
     2701 agcataaaaa tttcgtacaa caaacacttg ataccattat tgaattgggg ggagtggttt
     2761 tgagcgatga tgtaaccgat ttagtccaca tcaataatga atttaccgcc tcaatcgtat
     2821 taagtcgttg tcgcctaacg ccatcaggaa gcaaaagatg gttgatccga tttgatacca
     2881 gtttaaatcc cgacataaca attgcagtgc gcatcaatga gagtgccaca gaaattcttg
     2941 attactacct gcttcccaca actgaaaagg taaatgaaaa actacgattg gctgagagta
     3001 atcctgccga acttgaaatc taccgccatg ataatttaga tcgctttttt attatggttg
     3061 aacgtatttt agtcaaggag tttatctatg caaaacgaca ccattcaaat tcttcctatc
     3121 gacaaaattc gggtactcaa tcccagaacg cgcaatcaga aagtctttgc tgatctggtc
     3181 gaaaacattg caacgattgg tctcaagaga cctattaccg taacgccatc tgtcgatagt
     3241 gctgaatatt atgatttgtt atgcggtcaa ggtcggcttg aggcttgtca ggtgcttggt
     3301 gagctattca taccttgtcg cgttgtgaac gcaaatagtg aagaaagcta cttgatcagc
     3361 cttgtggaaa atattgccag acgtcagcat tcctatatgg agctgctttc aggcatcaaa
     3421 atactcaatg atagaggtta taagcccgtt gatattgctc gcaaaatcgg tttctcaaaa
     3481 agccatgtca acagtatctt acacctcatc aatgctgggg aagagcgcct tatcaatgcg
     3541 gtagagcaag gaattttacc cattaccatc gctatttcga tttctcgttc agacgatcag
     3601 gaagttcaaa aacaactcgc ggaactttac accaatggta cattgaaaag caccgatatt
     3661 gccaaaatac gtaacctgat ccaccgtaga aatcttgctg gtaagaaagc caatacaggt
     3721 atttctggaa gtattagatc atctatttat aatcccaaaa ctgtggtgaa tatctacaaa
     3781 gaggaaacgg aaagacagaa aatgatgatc aagcaagcgg actatgatga aaaacaactc
     3841 tctattatct tgtcttgctt aaataaactc tttgaggaca aatactttca actcgtcctt
     3901 aaatcagaac atttagacga tatgccaaaa gatttaagcc aacgaatgtt aaaacaacga
     3961 gagggaaata tttatgccta aacatattac ttatgccttt cataatcagt taatttcgct
     4021 tccaattgat gagattattc cattaaaaaa gatggataaa aattttgaga aaagcactaa
     4081 gttccaaagc attctggctt caattcgtga aatcgggatt gttgaacctg tggtcgtcta
     4141 tccagaagaa aacaaccaat atattttgtt agatggccat tctcgtgtac atgcactcaa
     4201 aaagctaggt gtggataagg tgctttgtat gatttctacc gatgatgaag gctttactta
     4261 caacaagcaa attaaccgct taaccgccgt tcaggaaaat aaaatgctga tgaaaaccat
     4321 tgagcgcggg gtttcggatg aactgatcgc aagaacactc aatatcaatc taaaagcact
     4381 acggcagaaa atgaatatgc ttaatgggat cacaagagag gttgttgata tattggccaa
     4441 taagcaggtc agtaaagaga tatttcggat tttgcgcaag atgaagcctg aacgccaaat
     4501 tgaagtggca caaatgatga tcgccagtaa taagtttaca ttgacatacg ccaatatgat
     4561 gttgctcagt tcacgcaagg aggaattagt cgaaagccac aagtctaaaa ccacgaatga
     4621 tgatttaacc gatctttcca ttatgcaaaa agagctagag cgacttaaag agaactataa
     4681 aatttccgaa gagaaattgg cggacttaaa aatgggattg gttgtggcga aagggtatgt
     4741 caaaaaacta ttgaataatg ctgccgtggt agatttgctt gaaaatgatc agagtgaaat
     4801 ctatcttact cttaaagaag tatgtgatat tcaataatat aatatctagc taataacctt
     4861 aat
//
LOCUS       DANLRK010000175         4808 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.175, whole genome shotgun sequence.
ACCESSION   DANLRK010000175 DANLRK010000000
VERSION     DANLRK010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4808
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.175"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            7..465
                     /locus_tag="PXA33_002237"
     CDS             7..465
                     /locus_tag="PXA33_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HDL5121536.1"
                     /translation="MLLSVTVNAAPVELHQFDSPKQESDYRALIQELRCPQCQNNNIA
                     DSNATIATDMRAKTLELLKQGKSKDEVVDYMIERYGNFVTYDPPLTPATIMLWLLPLI
                     LIALGLGVVLRRKKNKPQAVNFEQNTAKPTTHLAQDEQERLNQILNKEEK"
     gene            462..1373
                     /gene="ccmI"
                     /locus_tag="PXA33_002238"
     CDS             462..1373
                     /gene="ccmI"
                     /locus_tag="PXA33_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HDL5121537.1"
                     /translation="MNFWLAIIFVTLIVCGVAFYPLLKKNKQIDNTKRDSLNKAFYFD
                     RLKEVENEANEGVIDDPEQTKLELQKNLLDDIPHSTQSATSEKLTLGKSWFVALILFV
                     GGISFAIYSNVGSWFSNSMLDTTHQKLDSFYERLKTEETNPLSQEEMNQFAMALRVDL
                     QSNPNDDKKWYMLGQIGMALDDGQLAHDAFSKAAQLKPDNLDYKLSHAQLLLFSEAQE
                     DKKQGETLLKEIIRQDHTNFNALSLLAFHSFEKEDFKMAAMTWGMMLKLLPDEDPSKV
                     TVERSFNMALSNLSEAEKQELQKGEKK"
     gene            1370..1975
                     /gene="mog"
                     /locus_tag="PXA33_002239"
     CDS             1370..1975
                     /gene="mog"
                     /locus_tag="PXA33_002239"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HDL5121538.1"
                     /translation="MTACKPLSKEKLKIGLVSVSDRASQGVYSDQGIPELRAWLESAL
                     TEPFELETRLIPDEQAIIEQTLIELVDAHACHLVLTTGGTGPAKRDVTPDATLAVAHR
                     EMPGFGEQMRQVSLHFVPTAILSRQVGVIRHDSLILNLPGQPKAIKETLEGVKDEQGK
                     VLVKGIFSAVPYCLQLLSEIYVETHAAICESFRPKSARREN"
     gene            1985..2323
                     /gene="glnB"
                     /locus_tag="PXA33_002240"
     CDS             1985..2323
                     /gene="glnB"
                     /locus_tag="PXA33_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005654045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HDL5121539.1"
                     /translation="MKKIEAIIKPFKLDDVREALTDVGVSGMSITEIKGFGRQKGHTE
                     LYRGAEYAIDFLPKVKVEIVIPDELEEQCIEAIMETAQTGKIGDGKIFVYDVGRVIRI
                     RTGEENEEAI"
     gene            2404..2883
                     /gene="lspA"
                     /locus_tag="PXA33_002241"
     CDS             2404..2883
                     /gene="lspA"
                     /locus_tag="PXA33_002241"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005724521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HDL5121540.1"
                     /translation="MKTGLKFLWVSLIVIIIDLWSKYIVVQHFEFGESINILPIFNFT
                     YARNYGAAFSFLADHSGWQKYFFLGLAVVISSALVVALWKNQAVKKLENIAYALIIGG
                     AIGNAIDRAYNGYVVDFLHFYWDIYHYPVFNIADIAICVGAGLLILEAFITKKEPKK"
     gene            2894..3838
                     /gene="ispH"
                     /locus_tag="PXA33_002242"
                     /gene_synonym="lytB"
     CDS             2894..3838
                     /gene="ispH"
                     /locus_tag="PXA33_002242"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HDL5121541.1"
                     /translation="MKILLANPRGFCAGVDRAISIVELALEIHGAPIYVRHEVVHNKF
                     VVDGLKAKGAIFVEELHEVPDDAIVIFSAHGVSQVVRQEAKNRGLKVFDATCPLVTKV
                     HMQVARASKKGTKAILIGHEGHPEVIGTMGQYNNQEGGIYLVESVEDIANLPVSNNDE
                     LTFMTQTTLSIDDTIGVIEALKEKYPAIQGPRKNDICYATTNRQHAVRELAEQSQLVL
                     VVGSKNSSNSNRLAELASRMGVMSKLIDGPQDIDPTWLEGIETIGITAGASAPEVLVQ
                     SVISHLKTLGVTEVETLVGNEEDVVFEVPKELRIKEVK"
     gene            3838..4086
                     /locus_tag="PXA33_002243"
     CDS             3838..4086
                     /locus_tag="PXA33_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HDL5121542.1"
                     /translation="MTEINRFKLEWACRRGMRELDKMIMPFYQNHFNELNETQQQAFV
                     EMLNYSDPELFRWVMLQAPAPTEEIAELIRLMRDKIET"
     gene            complement(4293..>4808)
                     /locus_tag="PXA33_002244"
     CDS             complement(4293..>4808)
                     /locus_tag="PXA33_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL5121543.1"
                     /translation="SANQYIFNSDWFTHNVPSLASIFEYVKPRRILEIGSFEGRSSVF
                     FIEEALKYQSQVEIHCIDSWEGGAEHQGVWDMGEIEQRFIHNVTLAQQKFQNVVLYKH
                     RGYSHQKMIELLANGYINYFDYIYIDGSHEAPDVLFDALLAHRLVRKGGVISFDDYLW
                     SPDETGKQRHY"
BASE COUNT         1512 a          972 c         1041 g         1283 t
ORIGIN      
        1 ctaataatgc ttctaagtgt aacagttaat gctgctccgg tagaactgca ccaattcgac
       61 agcccgaaac aagagtcgga ctatcgtgcc ttaattcaag aactgcgttg cccgcagtgt
      121 caaaataata atatcgccga ttccaatgcg actattgcca ccgatatgcg ggcaaaaacc
      181 ttagaattgc tcaaacaagg caaaagcaaa gacgaagtgg tggattatat gattgaacgc
      241 tacggcaatt tcgtcactta cgacccgcct ctcacaccgg caaccattat gttatggctg
      301 ttgccgttaa ttttaattgc cttagggctg ggggtggtgt tgcgtagaaa gaaaaacaaa
      361 ccacaagcgg tcaattttga gcaaaatact gcaaaaccga ccactcatct cgctcaagat
      421 gagcaagaac gtcttaacca aattctcaat aaggaagaaa aatgaatttc tggctggcaa
      481 ttattttcgt tacactgatt gtatgtggcg tggcgtttta tccgttactt aaaaagaaca
      541 agcaaatcga taacaccaag cgagacagct taaacaaagc cttctatttt gaccgcttaa
      601 aagaagtgga aaacgaagcg aatgaagggg tgattgatga ccctgaacaa accaaattag
      661 agctacaaaa aaacttgctt gatgatattc cacacagcac acaaagtgcc acaagtgaaa
      721 aattaacgct cggcaagtcg tggtttgtgg cattgatttt atttgtcggc ggtatcagct
      781 ttgcgattta cagcaatgtc ggttcttggt tctccaactc aatgttagac accactcacc
      841 aaaaattaga cagcttctat gagcgtttaa aaaccgaaga gaccaatccg ctctcacaag
      901 aggaaatgaa ccaatttgcg atggcattac gggtagattt acaaagtaat ccgaatgacg
      961 acaaaaaatg gtatatgctc ggtcaaatcg ggatggcgtt agatgatggt cagcttgcac
     1021 acgatgcctt tagcaaagcg gctcaactca agccggataa cttagattac aaactcagcc
     1081 acgcccaact gctgctcttt tccgaagcac aggaagacaa aaaacagggt gaaaccttgt
     1141 taaaagagat tatccgccaa gaccacacaa actttaatgc gttaagttta ttggcgttcc
     1201 actcctttga aaaagaagat ttcaaaatgg cggcaatgac gtggggaatg atgttaaaac
     1261 tactgcctga tgaagatcct agcaaagtaa cggtagaacg cagttttaat atggcgttat
     1321 ccaatttaag tgaggcggaa aaacaagaat tgcaaaaagg tgagaaaaaa tgaccgcttg
     1381 taaaccactt agcaaagaga agttgaaaat cggcttggtg tcggtttcag accgagcctc
     1441 gcagggggtt tattcagacc aaggcattcc cgaattaagg gcgtggcttg aatctgcctt
     1501 aaccgagccg tttgagctgg aaacccgctt aattcccgat gagcaagcca ttattgagca
     1561 aaccttaatc gagttagtcg atgctcacgc ttgccatttg gtattaacca ccggcggcac
     1621 cggcccggca aaacgtgatg tcaccccaga tgccaccctt gctgtcgccc accgagaaat
     1681 gcccggcttt ggcgaacaga tgcgacaagt gagccttcac ttcgtgccaa ccgccattct
     1741 ctctcgccaa gtgggagtaa ttcgccacga cagtctgatt ttaaatctgc cggggcaacc
     1801 gaaagcaatt aaagaaacgc tggaaggtgt gaaagatgaa cagggcaaag tattggttaa
     1861 aggtattttt agtgccgtgc catactgttt acaactttta agcgaaattt atgtcgaaac
     1921 ccacgcagct atttgcgaaa gtttcagacc caaatcggca agacgagaaa attaaggcta
     1981 aaaaatgaaa aaaatagaag caattattaa accttttaaa ttagacgatg tgcgtgaagc
     2041 cttaaccgat gtgggtgtca gcggaatgag tattaccgaa atcaaagggt ttggcagaca
     2101 aaaaggacat accgagcttt atcgtggggc ggaatatgcg attgattttc taccgaaagt
     2161 gaaagtcgaa atcgtgattc ccgacgagtt ggaagaacag tgcattgaag caattatgga
     2221 aaccgcccaa accggcaaaa tcggcgatgg caagattttt gtgtatgatg tcgggcgggt
     2281 gatccgcatt cgtaccggcg aagaaaatga agaagcgatt tagttttgca aaaaatccgg
     2341 caaaaacgac cgcttgcagg gggatttcca tctactcttt ttacattaaa ttttggatat
     2401 taaatgaaaa caggcttaaa atttctttgg gtgagtttaa ttgtaatcat cattgacctt
     2461 tggtcaaaat acattgtagt gcaacatttt gaatttggtg aaagcattaa tattttgccg
     2521 atttttaact tcacttacgc ccgaaactac ggggctgcct ttagtttcct tgccgaccat
     2581 tccggctggc aaaaatattt ctttttaggg ctggcggtag tgatttccag tgctttagtg
     2641 gtggctcttt ggaagaatca agcggtcaaa aaattagaaa atattgcgta tgccttaatt
     2701 attggtggag cgattgggaa tgccattgac cgtgcttaca acggctatgt agttgatttc
     2761 cttcattttt attgggatat ttatcactac cctgtgttta atattgccga tattgcgatt
     2821 tgtgtgggtg caggcttact gattttagaa gcctttataa ccaaaaaaga accgaaaaaa
     2881 taagagtgat taaatgaaaa ttttattagc aaacccacgc ggtttttgtg ccggcgtgga
     2941 tcgagccatt tcgattgttg aattagcctt agaaattcac ggtgcaccta tctatgttcg
     3001 ccacgaagtg gtacataaca aatttgtggt ggatggcttg aaagccaaag gggctatttt
     3061 tgttgaggag ctacacgaag tgccggacga tgctatcgtg atcttctcag cccacggggt
     3121 gtcgcaagta gttcgccaag aagccaaaaa tcgtggctta aaagtgtttg atgccacttg
     3181 cccactcgtg accaaagtgc atatgcaggt tgcccgagcc agtaaaaaag ggacaaaagc
     3241 aattttaatc ggtcacgaag gtcaccctga agttatcggc acaatgggac aatacaataa
     3301 ccaagaaggt ggcatttatt tggtagaatc ggtggaagat attgccaact tgccggtttc
     3361 caacaacgat gaattaactt ttatgacgca aaccacactt tccattgacg ataccattgg
     3421 cgtgattgag gcgttaaaag agaaatatcc ggcaattcaa ggccctcgca aaaatgatat
     3481 ttgttatgct accaccaatc gccaacacgc agtgcgggaa ctggcagaac aatcacaatt
     3541 agtgttagtg gtaggttcaa aaaactcctc gaactcaaat cgtttggcgg aactcgcctc
     3601 tcgaatgggt gtgatgtcaa aactgattga tggtccgcaa gatattgatc caacttggtt
     3661 ggagggtatt gaaactatcg gtattaccgc cggtgcttcc gctcctgaag tgttggtgca
     3721 atcagtaatt tcgcatttaa aaacccttgg cgtaaccgaa gttgaaacct tagtcggcaa
     3781 cgaagaagac gtggtgtttg aagtcccgaa agagttacgc atcaaagagg taaaataatg
     3841 acagaaatca accgctttaa attagaatgg gcgtgccgac gtggtatgcg tgagttagac
     3901 aaaatgatta tgccgtttta ccaaaatcat tttaatgaac tcaatgaaac ccaacagcaa
     3961 gctttcgttg aaatgctcaa ttacagcgac ccggaactgt ttcgctgggt aatgctgcaa
     4021 gcacctgccc caaccgaaga aatcgctgaa ttgattaggt taatgcgaga taaaattgag
     4081 acctagagct aatagattaa cctcttcttt ggggtaaata tatagctact taccccaaag
     4141 aagactaaat tcattcaact tttacaaaat aaccctaaac tagaaaagtt aatcttccaa
     4201 tttaataata tgttgctgat aagctattaa accttgtatt acatgcactt tctgctgata
     4261 ggtattgaca tagtgatcaa cagcgggttt tactaataat gacgctgttt acctgtctca
     4321 tctggtgacc aaaggtaatc atcaaaacta ataacgcctc cttttctcac taaacgatgt
     4381 gcaagtaaag catcgaacaa cacatctggc gcttcgtgag atccatctat gtaaatatag
     4441 tcaaaataat ttatatatcc atttgcgaga agttcaatca ttttttggtg agaatagcca
     4501 cggtgcttat ataacacaac attttggaat ttctgttgcg caagagtgac attatggata
     4561 aatcgttgtt ctatttctcc catgtcccaa actccttgat gctctgctcc cccttcccaa
     4621 ctatcaatac aatgaatttc aacttgtgat tgatatttca atgcttcttc aatgaaaaag
     4681 acagatgatc taccttcaaa tgatccaatt tctaaaatcc tccgaggttt tacatattca
     4741 aaaatagaag ctaatgaagg aacattatga gtaaaccaat cagagttaaa aatatattgg
     4801 ttggctga
//
LOCUS       DANLRK010000176         4561 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.176, whole genome shotgun sequence.
ACCESSION   DANLRK010000176 DANLRK010000000
VERSION     DANLRK010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4561
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.176"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            139..4035
                     /gene="purL"
                     /locus_tag="PXA33_002245"
                     /gene_synonym="purI"
     CDS             139..4035
                     /gene="purL"
                     /locus_tag="PXA33_002245"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HDL5121544.1"
                     /translation="MTIQTFRGSPALSAFRIQQLLQKFQQNQLPVKSVYAEFLHFVAL
                     NRPLVADEENKIKELLHYGPTLEEDEPTGFCLIVTPRIGTISSWSSKATDIAHNCGLA
                     AVERIERGTAYYLEFSETPNESQLTTLKGLLHDRMMETVLDNESQAEQLFAQQEPKPF
                     TSVDILGGGRKALEEANINLGLALADDEIDYLVENFTQLGRNPNDIELYMFAQANSEH
                     CRHKIFNADWTIDGVKQEKSLFKMIKNTFEKTPDYVLSAYKDNAAVMEGSTVGRFFPD
                     QDGQYRYHQEDAHILMKVETHNHPTAISPFPGAATGSGGEIRDEGATGRGAKPKAGLT
                     GFSVSNLVIPKFEQPWENPLSKPNRIASALDIMIDGPLGGAAFNNEFGRPALLGYFRT
                     YEEKVNSFAGEEIRGYHKPIMLAGGIGNIRAEHVQKGEIPVGAKLIVLGGPAMNIGLG
                     GGAASSMASGKSKEDLDFASVQRENPEMERRCQEVIDRCWQMGAENPILFIHDVGAGG
                     LSNAMPELVHDGECGGKFDLRNILCDEKGMSPLEIWCNESQERYVLAVSPEKLDIFTA
                     LCERERAPFAVIGEATKEKHLTLADPHFDNKPIDLPMNVLLGKTPKMQRDVLSKTVKN
                     PPLATADIDLKEAFHRVLRLPAVAEKTFLITIGDRSVTGMVAQDQMVGPWQIPVADCA
                     VTTASLDSYHGEAMSIGERSPVALLDFAASARLAVAESITNIAATNIGDIKRIKLSAN
                     WMSAAGHEGEDAGLYEAVKAVGEELCPELGITIPVGKDSMSMKTTWEENGEQKSVTAP
                     LSLVISAFARVEDVRKTVTPQLRTDKGDTRLLLIDLGEGNNRLGATALAQVYKQLGDK
                     PADVVNVARLKGFFNAMQTLVAQGKLLAYHDRSDGGLITTLAEMAFAGNCGISVEIDR
                     LVDDLAVLFNEELGAVIQVRTEELSEVQAVLNAENLANLTFDIGTAVESDSFEITRNG
                     TTVFSQKRSELRGIWAELTHQMQRLRDNPECADQEFAAKKDPNNKGLSAFLTYDVNED
                     IAAPYINKGVKPSVAILREQGVNSHYEMAAVFDRAGFNAIDVHMSDLISGRRNLNDFN
                     AMVACGGFSYGDVLGAGGGWAKSILFNPMLRDQFSKFFANPNTLALGVCNGCQMVSNL
                     AEIIPGTENWPRFVRNKSERFEARAAMVKINDTNSLWFNGMAGSHMPIAVSHGEGQVE
                     FKNPQQLANLQQQNLVIAQYVDSHLNPTEQYPANPNGSALGITAIGNTDGRVAIMMPH
                     PERVFRAVSNSWCPEDWTEGGAWMRLFRNARVALKY"
     gene            4390..4561
                     /locus_tag="PXA33_002246"
                     /pseudo
     CDS             4390..4561
                     /locus_tag="PXA33_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248253.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
BASE COUNT         1280 a         1032 c         1121 g         1128 t
ORIGIN      
        1 ttcattagta ttgaacaaat ttatttgata ctaatgaata aatgtatcta acagcaaacg
       61 tctgacaaat atagcaatcg tttgcgcaat cgtttataat cggtttagtt tttcatctta
      121 aataggaaaa tccaaagaat gacaatccaa accttccgtg gctcgcctgc cttatctgct
      181 tttcgcattc aacaacttct gcaaaaattc caacaaaatc aattacctgt aaaatcagtt
      241 tatgcggagt ttttgcattt tgtggcatta aatcgaccgc ttgtagccga tgaagaaaac
      301 aaaatcaaag agttattgca ctacggtcca acgctggaag aagacgagcc gacagggttt
      361 tgcctgattg ttacccctcg tatcggcacg atttcctcgt ggtcgtctaa ggcaaccgac
      421 atcgcccata actgcggttt ggcagcagta gagcgaattg agcgtggcac ggcatattat
      481 ttagaatttt ctgaaacgcc gaacgaaagt caacttacga cgttgaaagg cttattacac
      541 gaccgtatga tggaaacggt gctggataac gaaagccaag ccgaacagct tttcgctcag
      601 caagagccga agccgtttac ttcggtggat attttaggcg gtggtcgcaa ggcactggaa
      661 gaagcgaata tcaacttagg tttagcgttg gcggacgatg aaattgatta cttggtggaa
      721 aactttaccc aacttgggcg taaccccaat gatattgaac tctatatgtt cgcccaagcc
      781 aactcggagc attgccgcca caaaattttt aatgcggatt ggacgattga tggggtaaaa
      841 caggaaaaat ccctgtttaa gatgattaaa aacacctttg agaaaacccc ggactatgtg
      901 ctttccgcct acaaagacaa tgcggcggtg atggagggct caacggttgg gcgtttcttc
      961 cccgaccaag atggacaata tcgctatcat caagaagatg ctcatatttt aatgaaagta
     1021 gaaactcaca accacccaac cgccatctcg ccattcccgg gagcggcgac cggttcgggc
     1081 ggtgaaattc gtgatgaagg ggcgacaggg cgtggggcga aaccgaaagc gggcttgacg
     1141 ggtttttcgg tctctaactt agtgatccca aaatttgaac agccgtggga aaacccgctt
     1201 tccaagccga atcgcattgc ctccgcactg gatattatga ttgatggccc actaggcgga
     1261 gcggcattta acaatgaatt tggtcgtcct gcgttattgg gctacttccg tacttacgaa
     1321 gaaaaagtga acagcttcgc aggcgaagaa attcgtggct atcacaagcc gattatgtta
     1381 gcaggcggta tcggcaacat tcgtgccgaa cacgttcaaa aaggcgaaat cccggtggga
     1441 gcgaagttga tcgtgcttgg tggcccggca atgaacatcg gcttaggcgg tggggcggca
     1501 tcgtcaatgg catcggggaa atcgaaagaa gatttggatt ttgcctcggt tcagcgggaa
     1561 aaccctgaaa tggaacgccg ttgccaagag gtgatcgacc gttgctggca gatgggggcg
     1621 gagaacccaa ttttatttat tcacgacgtg ggggctggcg gtttatcaaa tgcgatgcct
     1681 gaattggttc acgacggcga atgtggcggt aagtttgatt tgcgtaacat cttgtgcgat
     1741 gaaaaaggaa tgtcgccact tgaaatttgg tgtaacgaat cgcaagagcg ttatgtgctt
     1801 gccgtttccc ctgaaaaact cgacattttc acagcattgt gcgaaaggga aagagcccca
     1861 tttgcggtga ttggcgaggc aacgaaagag aagcatttaa cccttgctga ccctcatttt
     1921 gacaataaac cgattgattt gccgatgaat gtgttgctcg gcaaaacgcc aaaaatgcag
     1981 cgagatgttt tgtcaaaaac tgtgaaaaat ccaccgcttg caaccgctga catcgacttg
     2041 aaagaggcgt tccaccgtgt gctacgcttg cctgcggtgg ctgaaaaaac cttcctgatt
     2101 accatcggcg atcgctcagt aacgggaatg gtggcacaag atcagatggt cggcccgtgg
     2161 caaattcctg tggcagattg tgccgtaacc accgcttcat tagacagcta tcacggtgaa
     2221 gcaatgtcaa ttggcgagcg atcacctgtt gctttgcttg actttgctgc ctcagctcgc
     2281 ctcgccgtgg cagaaagcat caccaatatt gccgccacta acattggcga catcaaacgc
     2341 attaaactct cggcgaattg gatgtcggcg gcagggcacg agggcgaaga cgcagggctt
     2401 tacgaggcag taaaagcggt gggcgaagag ctttgccctg aattaggcat aaccattccg
     2461 gtaggcaaag actcaatgtc gatgaaaacc acttgggagg aaaacggcga acagaaatct
     2521 gttactgctc cgctttcgct cgtgatttcc gctttcgctc gtgtggaaga tgtgcgtaaa
     2581 accgttaccc cacaacttcg caccgacaaa ggcgataccc gcttgttgct gatcgacctg
     2641 ggcgaaggca acaaccgctt gggtgcaacc gcacttgctc aagtttacaa acaacttggc
     2701 gacaaacctg ccgatgtggt gaatgtggct cgcttaaaag gcttcttcaa cgcaatgcaa
     2761 acccttgtcg cacaaggaaa attgcttgcc taccacgacc gctcggacgg tggtttaatc
     2821 accacactgg cggaaatggc gtttgcaggg aattgcggaa tttcggtgga aatcgaccgc
     2881 ttggtggacg atttagccgt gttattcaac gaagaactgg gggcagtaat ccaagttcgc
     2941 accgaagaat tgagcgaggt gcaagcggtg ttaaatgccg aaaatcttgc aaatctcacc
     3001 tttgatattg gtacagcggt tgaaagcgac agctttgaaa ttacccgcaa cggcacaaca
     3061 gtatttagcc aaaaacgttc cgaactgcgt ggcatttggg cggaactcac tcatcaaatg
     3121 cagcgtttgc gagacaaccc tgaatgtgca gaccaagaat ttgcagcgaa aaaagacccg
     3181 aataacaaag gcttatccgc atttttaact tacgatgtga acgaagacat cgccgcccca
     3241 tacatcaaca aaggcgtaaa accgagtgtg gcgattttgc gtgagcaagg tgtaaacagc
     3301 cactacgaaa tggcagccgt ctttgaccgt gccggtttca acgccattga cgtgcatatg
     3361 tcagacttga tttcaggcag acgcaacctc aacgacttca acgcaatggt ggcgtgcgga
     3421 ggcttctctt acggcgatgt cttaggtgca ggcggcggct gggcgaaatc gattctgttc
     3481 aacccaatgc tgcgtgacca attcagcaaa ttcttcgcca acccgaacac gctggcatta
     3541 ggggtatgta atggttgcca aatggtctcc aatctagcgg aaattattcc aggcacagaa
     3601 aactggccac gcttcgtgcg taataaatcc gaacgttttg aagcccgtgc tgcaatggtg
     3661 aaaatcaacg acaccaactc actttggttc aacggaatgg caggcagcca tatgccgatt
     3721 gcggtttcac acggtgaagg acaagttgag ttcaaaaatc cacagcaatt agcgaacttg
     3781 caacagcaaa atctcgtgat cgcacaatat gtggatagcc acctcaaccc aaccgaacaa
     3841 tacccagcca acccgaacgg atcggcgttg ggtatcaccg ctatcggtaa tacagacgga
     3901 cgagtcgcta ttatgatgcc acaccctgaa cgtgtgttcc gtgcggtcag taactcttgg
     3961 tgcccagaag attggacgga aggtggagcg tggatgcggt tgtttagaaa tgcgagagtg
     4021 gcgttgaagt attaaaaatc taccttcgta gtgatctgcc cccatagggg gcgattaaaa
     4081 ttcgaattca tagaacccga atttttatat atctgataaa cgaagttgtt tatccttcgt
     4141 gcttttcctg atatttcaag tactttctta tcatttctgc attgacatct acggtgtcta
     4201 cgcagtagcc tcttgccaca aaagtgggtt gccccacaat ttatgcttac gtaaataggg
     4261 aaacttattg aaaaatctta ttggaccctg ttcacccttt taaatgcccc attacgtctt
     4321 atacagaaaa tgaacgtgat ttatttgaac ggtcaattct accttaatag aaagtggttt
     4381 ttagttagca atcaaaatca cattaacgga attgagaatt tggagagtca ggcaaaatgc
     4441 tatttgcgta aatttaacgg cgttcctaaa gtacatgaat tctatttgaa agaatgcaga
     4501 tggcgtttta atcacagtaa cttaaaatat caagttttct ttttaaaaca attggttaaa
     4561 t
//
LOCUS       DANLRK010000177         4550 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.177, whole genome shotgun sequence.
ACCESSION   DANLRK010000177 DANLRK010000000
VERSION     DANLRK010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4550
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.177"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(218..1621)
                     /locus_tag="PXA33_002247"
     CDS             complement(218..1621)
                     /locus_tag="PXA33_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDL5121545.1"
                     /translation="MLTYTFNTQEGTALYEQLYRFLKNDIESNILIAGDKLPSKRAFA
                     KHLGISVMTVETAYQQLVAEGYVNTQPKKGFFVNALNLPEKKEAISVQLQKISSEQYE
                     KNKKWQADLTNRQTLAENFPFSVWTKLVREVLKTHQSELMERAESGGVWTLKFAIANH
                     LRAFRGMIVNPTQIIIGAGTEYLYGLLIQLLGLDKHYALTEPSYDKLRRIYHSYRLST
                     ATVSMPFSIDELNSKNVDVIHTSPSHHFPTGHVMPIAKRYELLAWVAQKAERYIVEDD
                     YDSEFRFVGQPIPALKSIDMLGRVIYMNTFSKTLSSTVRIAYMVLPPELLNRFQQELG
                     FYASTVSNFEQYILAEFIQQGYFEKHINRMRAYYQKKRDKLLKAFKQGNLAEKIAIKE
                     EHSGLHFIVQLNTTLTERQILQAAEEQGIKFVALSAYYQDKSQIQQNAFIIGYSNLKD
                     DQINYVAESLKKILDNE"
     gene            1832..2704
                     /gene="pdxS"
                     /locus_tag="PXA33_002248"
     CDS             1832..2704
                     /gene="pdxS"
                     /locus_tag="PXA33_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HDL5121546.1"
                     /translation="MTNRYDLNRQLAQMLKGGVIMDVTTPEQACIAEEAGACAVMALE
                     RIPADIRAAGGVSRMSDPKMIKSIQAAVSIPVMAKVRIGHVTEAQILQAIEIDYIDES
                     EVLSPADDTFHINKRKFEVPFVCGAKNLGEALRRINEGAAMIRSKGEPGTGNVVQAVH
                     HLRKMKQEIARLVSHTTDELYHVAKELQVPFDLVQYVHQYQKLPVVTFAAGGVATPAD
                     AALMMQLGAEGVFVGSGIFKSGDPAKRARAIVQAVTNYNDPKLLAELSEDLGEAMVGI
                     NEQEIELLMAARGV"
     gene            2704..3276
                     /gene="pdxT"
                     /locus_tag="PXA33_002249"
     CDS             2704..3276
                     /gene="pdxT"
                     /locus_tag="PXA33_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HDL5121547.1"
                     /translation="MKIAILALQGAFSEHTDKLQQLGVESMEIRQLADLNQDFDGLIL
                     PGGESTVQGKLLRELGLLEPLRQKILNGLPTLGTCAGLILLAENLVNDDKKHLATLPV
                     TVKRNAYGRQLGSFLVEEEVKYLGKIPLTFIRAPYIESTGKDVEILAKISGKIVGVKY
                     QNQIGISFHPEVNNDLSFHRYFVEMCRKNK"
     gene            3425..4375
                     /gene="tal"
                     /locus_tag="PXA33_002250"
     CDS             3425..4375
                     /gene="tal"
                     /locus_tag="PXA33_002250"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HDL5121548.1"
                     /translation="MSQLDTLSKMTVIVADTGDIEAIKLYKPKDATTNPSLILSASAL
                     PQYAPLIEEAINYAKAQSSDKDQQLVDAEDKLAVNIGLEILKLVPGRISTEVDARFSY
                     DTEKTIAKARKLIGLYNDAGISNDRILIKIASTWQGIKAAEQLEKEGINCNLTLLFSQ
                     AQARACAEAGVYLISPFVGRILDWYKAHEGKEYAPAEDPGVISVTNIYNYYKQHGYDT
                     IVMGASFRNVGEIIELAGCDRLTISPPLLKELQESDEKLVCKLDYKGEIKPRPEKMTE
                     AEFYWEHNQEPMAVEKLAEGIRKFAIDQEKLEAMLAEKLQ"
BASE COUNT         1464 a          820 c          882 g         1384 t
ORIGIN      
        1 attgtctgat aaattgttaa agaacaaaaa gaaacgacgc actggaaatc aattttaaat
       61 cgttcacaac agtgcgtcgt tgtgtgccat gcattatacg caaaacaaaa tgccttgcaa
      121 ggagaatttg gtaatttttt gcaaaaatca ctaaaataat taccacttgc gtgtttttaa
      181 ggcaatttgc taaaaattaa agcagttttg atacttgcta ttcgttgtct aaaatttttt
      241 tcaggctctc tgctacataa ttaatctgat catcttttaa attagagtac ccaattataa
      301 aagcattttg ctgaatttga gatttatctt gataataagc agacaatgcg acaaatttta
      361 taccttgttc ttctgctgcc tgcaaaattt gcctttctgt taatgttgtg tttagctgta
      421 caatgaaatg caagcctgaa tgttcttctt ttattgcaat cttttctgct aaattgcctt
      481 gcttgaaagc ttttagtaat ttatccctct ttttttgata atacgctcgc attcgattga
      541 tatgcttttc aaaatagcct tgttgaataa attctgccaa aatatattgc tcaaaattgg
      601 aaacggtaga agcataaaac cctaattcct gctgaaaacg attcagtaat tctggtggca
      661 aaaccatata ggcaattcgt acagttgaag agagcgtttt agaaaaagta ttcatataga
      721 taactcgccc gagcatatca atagatttca atgcaggaat tggttgtcct acaaaacgaa
      781 attcgctgtc gtagtcgtct tctacaatat atctttcagc tttttgtgca acccaagcta
      841 acaactcata acgtttggca atcggcatta cgtgtcctgt cggaaaatgg tgtgatggcg
      901 aggtatgtat cacgtccaca ttctttgaat ttaactcatc gattgaaaat ggcatcgata
      961 ctgtggctgt tgataagcga taactgtgat aaattctgcg caatttatcg taactgggct
     1021 cagttagtgc ataatgttta tctaagccta gaagctgaat gagcaaaccg taaagatact
     1081 ccgtacccgc tccaataata atctgtgttg gattaacgat cattcctcta aaggctcgaa
     1141 gatgattggc tatcgcaaat tttaatgtcc atacgccccc actctcagca cgctccatta
     1201 gctcactttg gtgtgttttc agcacttcgc gtactaattt tgtccaaacc gaaaacggaa
     1261 aattttctgc taaagtctgt cgattagtta aatctgcctg ccatttttta tttttctcat
     1321 actgttctga actgattttt tgtagctgaa ctgatatggc ttcctttttt tctggtaaat
     1381 ttaaagcatt cacaaaaaag ccttttttag gctgagtatt cacataacct tctgctacta
     1441 actgttggta agctgtttcc actgtcatta ctgaaatgcc taaatgcttt gcaaaagccc
     1501 gtttagacgg cagtttatcc cctgctataa gtatgttgct ttcaatgtcg ttttttagaa
     1561 aacggtaaag ttgctcatag agagcggtgc cttcttgtgt gttaaaagtg taggtaagca
     1621 taatctgaca tgataagaaa aacataaaat ggtcatttta aacagttcag attaaattac
     1681 aatacatgct gtttcctcac tcaaattggc tgagtgtatt taaattggta gatgaacact
     1741 cgcatccagc gaatataaaa ataaacttaa tgccgagcta atcgaaacta cgaaaagttc
     1801 aattattcaa caattagaag gaaaaagtac aatgactaat cgctatgatt taaacagaca
     1861 acttgctcaa atgttaaaag gcggtgtaat tatggatgta actacccctg agcaagcctg
     1921 cattgcggaa gaagctggag cttgtgcagt aatggcacta gagagaattc ctgcagatat
     1981 tcgtgcagct ggtggcgttt ctcgtatgag tgatcccaag atgataaaga gtatccaagc
     2041 agcggtatcc attcctgtaa tggcgaaagt acgtattgga cacgttacag aagcacaaat
     2101 cctgcaagcg atcgaaattg actatattga cgaaagtgag gtgctttctc ctgcagatga
     2161 taccttccac attaacaagc gtaaatttga agtgccattt gtatgtggag caaaaaattt
     2221 aggcgaagcc ctacgtcgta ttaatgaagg cgctgctatg atccgtagta agggcgagcc
     2281 aggaacaggc aatgtggtgc aagcagtaca tcatcttcgc aaaatgaaac aagaaattgc
     2341 ccgcttggtt tcacatacca cagatgaact ttatcacgta gctaaagagc tccaagtacc
     2401 atttgattta gtacaatatg tacatcagta ccaaaaattg cctgttgtca cctttgctgc
     2461 tggaggtgtt gcaacccctg ccgatgctgc attgatgatg cagttaggtg cagaaggagt
     2521 cttcgttggc tcaggtattt tcaagtcagg cgatcctgct aaacgagcgc gggccattgt
     2581 acaagctgtt accaactaca acgatcctaa attacttgcg gagctttctg aagatttagg
     2641 tgaagcaatg gtaggaatta atgagcaaga gattgaattg ctaatggcag caagaggtgt
     2701 ctaatgaaaa ttgctattct tgctctgcaa ggtgcattta gcgagcatac tgataaactc
     2761 cagcaactag gggttgaaag tatggaaatt cgtcaacttg ctgatttaaa ccaagatttt
     2821 gatggtctga ttttacctgg tggcgaatcg actgttcaag gtaaattatt acgtgaatta
     2881 ggcttgcttg agccgcttcg tcaaaaaatt ttaaatggct tgccaacgct tggaacttgt
     2941 gcgggtttaa ttttgcttgc ggaaaatttg gtaaacgacg ataaaaaaca tttggctact
     3001 ttgcctgtaa cagtgaaacg taatgcttac ggaaggcaat taggctcgtt tttagtagaa
     3061 gaagaagtta aatatctcgg taaaattcct ctcacattca ttcgtgcgcc ttatatagaa
     3121 agcactggaa aagatgtaga aattttagcc aagataagcg gtaagattgt tggagtaaaa
     3181 taccaaaatc agataggtat atccttccat cccgaagtga ataatgattt aagttttcac
     3241 cgctattttg tggaaatgtg ccgaaaaaat aagtaaaaga aaaggggcta ttgcctctta
     3301 ttttttatac ttttttagaa aaagtagttt tgaataaaaa aatactaaaa ctctatttcc
     3361 cttatgttat tattatagta acttttacta ctatttattt cacaatcaaa aaaggaatca
     3421 gactatgagc caacttgaca ccttaagcaa aatgaccgtt atcgttgctg acactggtga
     3481 tattgaagca atcaaacttt ataagccaaa agatgcaaca acaaacccgt ctttaatttt
     3541 aagtgcatcg gcattaccgc aatatgctcc attaattgaa gaagcgatta attacgcaaa
     3601 agcacaaagt agcgataaag atcaacagtt agttgatgct gaagataaat tagcggtaaa
     3661 tattggttta gaaatcttaa aacttgtacc tggacgtatt tctacagaag ttgatgctcg
     3721 tttttcttat gatactgaaa aaactatcgc taaagcacgt aaattaattg gattatataa
     3781 cgatgcaggc atcagtaatg atcgcatttt aattaaaatt gcctctactt ggcaaggtat
     3841 aaaggcagca gaacagttag aaaaagaagg cattaactgt aacttaacgt tattattctc
     3901 tcaagctcaa gctcgtgcgt gtgcagaagc aggggtttac ttaatctctc cattcgttgg
     3961 tcgtatttta gactggtaca aagctcacga aggtaaagaa tatgctccag ctgaagatcc
     4021 aggtgtaatt tcagtaacca atatatacaa ttactacaaa caacacggtt atgacactat
     4081 tgtaatgggt gcaagtttcc gtaatgtagg agagatcatt gagttagctg gttgcgaccg
     4141 tttaaccatc tcaccaccgt tattaaaaga gctacaagag agcgatgaaa aattagtttg
     4201 caagctagat tacaaaggtg aaatcaagcc tcgtccagaa aaaatgacag aagcagaatt
     4261 ctactgggag cataaccaag agccaatggc agtagaaaaa ttagctgaag gcattcgtaa
     4321 atttgcgatt gaccaagaga agttagaagc aatgcttgct gaaaaacttc aatagacaac
     4381 gcataaaaaa accggcatta acgataatta atgccggttt taatttctat aaatgattat
     4441 ttaaactcat aacgtctaat cgtttttaga tcgctatctt catttttaat ttcatcggca
     4501 agatgaacac gagcaccaac cgctgcatgt gcatcatact cacgagttag
//
LOCUS       DANLRK010000178         4455 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.178, whole genome shotgun sequence.
ACCESSION   DANLRK010000178 DANLRK010000000
VERSION     DANLRK010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4455
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.178"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            52..1320
                     /locus_tag="PXA33_002251"
     CDS             52..1320
                     /locus_tag="PXA33_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HDL5121549.1"
                     /translation="MTQQIEHSTWQSKFAAMGPGILMASAAVGGSHIIASTQSGAIYG
                     WQLAIIILLANLFKYPFFRFGVQYTLASGKTLLEGYKEKGKFYLIIFFILNVFAAMIN
                     TAAVAIVSAAILKFIFSALGIPLDLSIPMAGTIVIVVTWGILLLGQYKFLDTLSKWIM
                     IALTISTVSAVIIAALKGGVEIAPDFVEPSPWNLAALVFIVALMGWMPAPIEISAINS
                     MWVIAKKRLSKVNYEDGIFDFNVGYIGTAILAIVFLALGALVQYGSGEKVEQAGVAYI
                     AQLIKMYAFAIGDWSKLLIAFIAFMCMFGTTITVIDGYSRANAEALRLISGRKQHSQT
                     ELSIWMTVTALIGILIISQFMSDVAKMLQFAMISSFVSTPVFAWLNFSLVLKGEHRAK
                     GWLFWLAVIGLIYLTSFTLLFLAQQFKLIG"
     gene            complement(1383..3440)
                     /gene="ligA"
                     /locus_tag="PXA33_002252"
     CDS             complement(1383..3440)
                     /gene="ligA"
                     /locus_tag="PXA33_002252"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HDL5121550.1"
                     /translation="MSLFNQTSLFEENATPELVEKIETIRQTLRQYEYEYHVLDNPSV
                     PDAEYDRLMNELKNLEWQHPELISADSPTQRVGAKPLAGFAQVTHEIPMLSLDNAFSD
                     EDLDGFLRRMESYITASPSQLEFCCEPKLDGLAVSILYENGILTRAATRGDGTTGEEI
                     TANIRTIRNIPLKLNTTNPPARLEVRGEVFMPQSGFNELNEKALAKGEKTFANPRNAA
                     AGSLRQLDPKITRQRPLVLNAYGIGVYESDDELPATHFERLQWLKSLGIPVNNEIRLV
                     QGKAALLAYYAEIQAKRPTLGYDIDGTVLKVNDIALQEQLGFISRSSRWAIAYKFPAQ
                     EEMTILKEVDFQVGRTGAITPVAKLEPVFVAGVTVSNATLHNGDEIERLGIVIGDTVI
                     IRRAGDVIPQITGVMLERRPENAKKIEFPTACPICHSAVTRVEGEAVARCTGGLFCEA
                     QRKEALKHFVSRKAMDIDGVGEKLIEQLMARELIETPADLFKLDHTTLMRLERMGEKS
                     AQNALNSIEKAKNTTLPRFLFSLGIREVGEATALNLANHFGSLEAIRQATFEQLKEVQ
                     DVGEVVANRIVSFWQEPHNVEVVEDLIAQGVRWENVVKAEIVDNPLKDKNVVLTGTLT
                     QLTRDEAKALLQSLGCKVSGSVSSKTDYLIAGEKAGSKLAKAQELGIQILSEQQFIEL
                     TQH"
     gene            complement(3505..4407)
                     /gene="zipA"
                     /locus_tag="PXA33_002253"
     CDS             complement(3505..4407)
                     /gene="zipA"
                     /locus_tag="PXA33_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HDL5121551.1"
                     /translation="MELHIIFFILAGLLIAVLIGFSLWSARREKSNIFSNTFSTRPIA
                     VPPQNNKMSNSIPTSLQPQNEDYQAMSVPEENPQQIQQEVESSLQNIKISLPGGNPQQ
                     QPEQIQPIQSVAEPTVSTYGQKISNQQHIQYNTPDFANPMVNTVVEETPQAVAIEPQE
                     TQSAEEENPFVTLYLYAPQNEAFRGDFVVHYLEEVGLRFGEHQIFHRHQHIDDSLSPI
                     IFSAANLMNPGTFDLNNISNFATAGLVLFMQLPTEGNDVANMKLLISNAEHLAASLGG
                     FLYDDNQQPFGEESRQRYMQRVMH"
BASE COUNT         1148 a          890 c          993 g         1424 t
ORIGIN      
        1 aagtagaatg aaaccatctt gttttgatag taatgtgata aggaaagcta tatgacacaa
       61 caaatagaac attcaacttg gcaaagtaaa tttgctgcaa tggggccggg gattttgatg
      121 gcatctgcgg cagtgggtgg ttcgcatatt attgcctcta cacaatctgg tgcgatttat
      181 ggctggcagc tggcgattat tattctgtta gccaatttat ttaaatatcc gtttttccgc
      241 tttggtgtgc aatatacctt agctagcggt aaaactttat tggaaggcta caaagaaaaa
      301 ggaaagtttt atcttattat tttctttatc cttaacgttt ttgctgcaat gattaacaca
      361 gctgcggtgg cgattgtttc agcagcgatt ctaaaattta ttttttctgc tttaggtatt
      421 ccgttagatt taagcattcc aatggcagga actattgtga ttgtggtcac ttggggtatt
      481 ttacttctag ggcaatataa attcttagat acattatcta aatggattat gattgcttta
      541 accatctcta cggtatctgc cgtgatcatt gctgctttaa aaggtggcgt tgaaattgct
      601 ccagatttcg tagagccttc gccgtggaat ttagcagcac ttgtgttcat cgttgcatta
      661 atgggctgga tgcctgcacc aattgaaatt tctgccatta actcaatgtg ggttattgcg
      721 aaaaaacgtt taagtaaagt gaattatgag gacggtattt ttgattttaa tgtgggttat
      781 atcggcacgg caattttggc gattgttttt ttagcattag gtgcgttagt tcagtacggt
      841 tcaggtgaga aagtagaaca agccggtgtt gcctatattg ctcaattaat caaaatgtat
      901 gcttttgcga ttggtgactg gtctaagctt ttaatcgcat ttattgcatt tatgtgtatg
      961 ttcggcacaa cgatcactgt tattgatgga tattcacgag cgaatgcaga agcgttgcgt
     1021 ttaattagcg ggcgtaagca acattcacaa acagaattaa gtatttggat gacggtaact
     1081 gcactcatcg gcattttaat tattagccaa tttatgagtg atgtggcgaa aatgttacaa
     1141 tttgcaatga tttcatcatt tgtttctacc cctgtttttg cgtggttaaa tttctcttta
     1201 gtgctaaaag gtgagcatcg tgcaaaaggc tggttatttt ggttggcagt tatcggctta
     1261 atttacttaa ctagctttac gttactgttt ttagctcagc agtttaaatt gattggttaa
     1321 tgcttttaag cattcaatag atagaataag gggcagattt caatcatctg cccctttgtt
     1381 tgttaatgtt gagtaagctc aataaattgt tgctcgctta aaatctgaat tcccaactct
     1441 tgagcttttg ccagcttgga accggctttt tcgccggcga ttaagtaatc ggttttgctg
     1501 gaaaccgagc cggagacttt acagcccaaa ctttgtaata gggctttggc ttcatcacgg
     1561 gtgagctggg ttagcgtgcc ggttaagacc acgtttttat cttttaacgg gttatctaca
     1621 atttcggctt tcacaacgtt ttcccaacgt acaccttgag cgatcaaatc ttccaccact
     1681 tccacattat gcggttcttg ccagaagctg acaatacggt tggcaacaac ctcgcccaca
     1741 tcttgcactt cttttaattg ctcaaaagtg gcttggcgga tcgcctctaa gctaccaaaa
     1801 tggttggcta agttgagagc ggttgcctcg cccacttcac gaatacctaa cgagaataaa
     1861 aagcgaggaa gagtagtgtt ttttgctttt tcgatgctgt tgagagcatt ttgagccgat
     1921 ttttccccca ttcgctctaa tcgcattagg gtagtgtgat cgagcttaaa cagatcggca
     1981 ggcgtttcaa tcagctctcg agccattaac tgctcgatta gtttttcgcc tacgccgtca
     2041 atatccattg ccttgcgtga aacgaaatgt ttcaaggctt ctttacgttg agcttcgcag
     2101 aataaaccac cggtacagcg tgctactgcc tcgccttcta cccgagtaac agcggagtga
     2161 cagataggac aagcggtcgg aaattcaatt ttttttgcat tttccgggcg gcgttctaac
     2221 atcacgcctg taatttgcgg aattacatcg cccgctcggc gaataattac cgtatcgcca
     2281 atcacaatac caagtcgctc gatttcatca ccgttgtgta gcgttgcgtt acttaccgtt
     2341 acgccagcga caaaaacagg ctctaatttc gccacagggg tgattgcccc tgttcttccc
     2401 acttggaagt ccacttcttt gaggattgtc atttcttctt gagccggaaa tttatacgca
     2461 atcgcccaac gagaggagcg agagataaac ccgagttgct cttgtagggc aatgtcattt
     2521 actttcaata ctgtgccatc aatatcatag cctaatgttg ggcgtttagc ctgaatctcc
     2581 gcataataag ccaagagagc agctttgcct tgaaccaagc ggatttcatt atttacagga
     2641 ataccaagcg atttcagcca ttgcaaacgt tcgaaatggg tggctggcag ttcgtcatcg
     2701 ctttcataca ccccaattcc gtaggcattc agcactaacg gacgctggcg ggtgattttc
     2761 ggatctaact ggcgaagcga acctgctgcg gcattgcgag ggttggcaaa ggttttttcg
     2821 cctttagcta aggctttctc attcagctca ttaaagcctg attgtggcat aaagacttcg
     2881 cctcgcacct ctaatcgagc aggtggatta gtcgtgttga gctttaacgg aatattgcga
     2941 atggtgcgga tattagcggt aatttcttcg ccggttgtgc catcgccacg ggttgcagct
     3001 cgggtaagta taccgttttc gtataaaata ctcactgcca agccgtctaa tttaggttcg
     3061 caacaaaatt ccagttgcga aggagaggcg gtgatatagc tttccatacg gcgtaaaaag
     3121 ccatctaagt cttcatcgga gaaggcatta tccaacgaaa gcatagggat ttcgtgggtg
     3181 acttgagcaa agcctgccag cggtttggca ccaacccgtt gagtcggcga gtcggcacta
     3241 atcagttccg ggtgctgcca ttccagattt ttcagctcgt tcattaaacg gtcgtattcg
     3301 gcatcgggta cgcttgggtt atctaaaacg tgatattcat actcgtattg acgcaatgtt
     3361 tggcgaatag tttcgatttt ctcgactaac tcgggcgtgg cattctcttc aaataaagag
     3421 gtttggttaa ataaagacat tggtaaaatt ctctacaaaa aagaccgctt gtacaagcgg
     3481 tcggatttat taattttttc gcaattagtg catcacacgt tgcatataac gttggcggga
     3541 ttcttcgcca aaaggctgtt gattatcgtc atataagaat ccccctaacg atgctgctaa
     3601 gtgttcagcg ttactgatta acaacttcat attagccaca tcattgcctt cggttggaag
     3661 ttgcataaat agcactaaac cagctgtcgc aaagtttgag atattattta aatcaaaggt
     3721 tcctgggttc attaaatttg ctgcactgaa aataatcgga cttaatgaat catcaatatg
     3781 ttgatggcgg tggaaaattt ggtgttcgcc aaaacgtaag ccaacttctt ctaaataatg
     3841 tactacaaaa tcaccgcgga aagcttcatt ttgtggtgcg taaagataga gggttacaaa
     3901 tggattttct tcctctgccg attgggtttc ttgaggttcg atagctactg cttgaggtgt
     3961 ttcttccacc actgtgttca ccattgggtt tgcaaaatcc ggcgtattgt attggatatg
     4021 ttgctgatta gaaattttct gaccgtaagt gctaaccgtt ggttctgcta ccgattgtat
     4081 cggctgaatt tgctcaggct gttgctgagg atttccgccc ggcaaactga ttttgatatt
     4141 ttgcagcgag ctttcaactt cctgttgtat ctgttgcgga ttttcttccg gcacagacat
     4201 tgcttgataa tcctcatttt gtggctgtaa tgaggttggg atgctattgc tcattttatt
     4261 attctgtggt ggcacagcaa taggtctggt cgagaatgta tttgaaaaaa tatttgattt
     4321 ttcacgacga gcggaccaaa ggctaaatcc aattagtaca gcaatcagta gcccggcaag
     4381 aataaaaaaa ataatatgca gttccattgt gtcctcttta tcaaataatc aataaaaaat
     4441 gtattttcat atttg
//
LOCUS       DANLRK010000179         4451 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.179, whole genome shotgun sequence.
ACCESSION   DANLRK010000179 DANLRK010000000
VERSION     DANLRK010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4451
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.179"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(56..1195)
                     /gene="dnaJ"
                     /locus_tag="PXA33_002254"
     CDS             complement(56..1195)
                     /gene="dnaJ"
                     /locus_tag="PXA33_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015939959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HDL5121552.1"
                     /translation="MAKKDYYEVLGLSKGASEKDIKRAYKRLAAKHHPDKNQGSKESE
                     EKFKEITEAYDVLTDSEKKAMYDQYGHAAFEQGGAGAGGFGGFGGGGFGGFEDIFSEM
                     FGGGFGGGGRRQRVVRGDDLRYDIEITLEEAVKGCKKDIRIQTLAECDTCHGTGAEAG
                     SKVETCSHCHGSGRVRRQQGFFVTEAVCPSCHGTGKRIEKPCRSCHGDGRVQKAKNLS
                     VTIPAGVDTGNQLRLSREGAAGENGAPAGDLYVVIHVKEHDIFERDGSNLYCEVPISF
                     TLAALGGEIEVPTLDGKLKLKIPAETQTGKLFRVRGKGVTSPRGGYAGDLICKVVVET
                     PVNLTEEQKALLRQFEESLEGQGKHRPKHEGFFDGVKKFFDNLGK"
     gene            complement(1418..1720)
                     /locus_tag="PXA33_002255"
     CDS             complement(1418..1720)
                     /locus_tag="PXA33_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121553.1"
                     /translation="MLKLYPTTENVQPPFYKIYDHKSGETVIATETENSNTAILMDDF
                     VWFEQIPTTEALLADTWSVIENRSKQGLLKRAYTKDFEKHLADGKVEIYIAVNPHC"
     gene            complement(1862..3760)
                     /gene="dnaK"
                     /locus_tag="PXA33_002256"
     CDS             complement(1862..3760)
                     /gene="dnaK"
                     /locus_tag="PXA33_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005670448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HDL5121554.1"
                     /translation="MGKIIGIDLGTTNSCVAVMDGDKPRVIENAEGARTTPSIIAYTD
                     KETLVGQPAKRQAITNPKNTLFAIKRLIGRRFTDAEVQRDIEIMPFEISKADNGDAWV
                     TVKGDKLAPPQISAEILKKMKKTAEDFLGEPVTEAVITVPAYFNDAQRQATKDAGRIA
                     GLDVKRIINEPTAAALAYGLDSKKENQTIAVYDLGGGTFDISIIEIDNFDGEQTFEVR
                     ATNGDTHLGGEDFDNRVINYLVEEFQKEQGVDLRNDPMAMQRVKEAAEKAKIELSSAQ
                     ETEVNLPYITADATGPKHLNIKVTRAKLESLVEDLVNRSLEPLKTALADAGLSVGDIN
                     DVILVGGQTRMPLVQKKVADFFGKEPRKDVNPDEAVAIGAAVQGGVLSGSVTDVLLLD
                     VTPLSLGIETMGGVMTTLIEKNTTIPTKKSQVFSTAEDNQSAVTIHVLQGERKRAADN
                     KSLGQFNLEGINPAPRGMPQIEVTFDIDANGIINVSAKDKNTGKEQQIKIQASSGLSD
                     AEVEQMVRDAEANAEADKKFEELVQARNQADGIAHATRKQITEAGDALNADDKAKIEA
                     AISELETAAKGEDKAEIEAKIEALIKASEPLMQAAQAKAQGGEQPQQSQKDDGVVDAE
                     FEEVKDNK"
     gene            4019..4357
                     /locus_tag="PXA33_002257"
     CDS             4019..4357
                     /locus_tag="PXA33_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL5121555.1"
                     /translation="MKLITTIIQPSKLDQVQDALSEIGIQGMTITEVKGFGRQYGHTE
                     QELSQMSRIDFRPKIKVEILVSNYQTDEVIDAILNASYSGQVGDGKIYVANIEQVIRT
                     RTGETDDEAV"
BASE COUNT         1136 a         1025 c          885 g         1405 t
ORIGIN      
        1 caaagaatag ataataaaaa agtacatatt taagaaatat gtacttttta tgattttatt
       61 tgcctaagtt atcaaagaat ttcttcacac catcaaagaa accttcgtgt ttcggacgat
      121 gtttaccttg tccttctaaa ctctcttcaa attgtcgtaa tagtgctttc tgttcttcgg
      181 ttaaattcac aggcgtttcg accaccactt tgcagattaa atcccctgcg tagccaccac
      241 gagggcttgt tacccctttg cctcgtacac ggaatagttt accggtttga gtttctgccg
      301 gaattttgag ttttaatttg ccgtctaaag ttggcacttc aatttcaccg cctaaagctg
      361 caagggtaaa gctaatcggc acttcacagt ataaattcga accgtcacgc tcaaaaatat
      421 cgtgttcttt aacgtgaatc accacatata aatcgcctgc cggtgcgcca ttttcgcctg
      481 cagctccctc tctcgataag cgaagttgat taccggtatc cacacctgcc ggaatggtaa
      541 cagagaggtt tttcgctttc tgtacacgtc catcgccgtg gcaagaacgg cacggttttt
      601 caatgcgttt acctgtaccg tgacaacttg gacaaactgc ctcagtcaca aagaaacctt
      661 gctgacggcg aactctgccg gagccgtgac aatgcgaaca ggtttccact ttgctccctg
      721 cttccgcccc tgtaccatga caagtatcac attcagctaa ggtttgaatg cgaatatctt
      781 ttttacagcc tttcacagct tcttcaaggg tgatctcgat gtcgtaacgt aaatcatcgc
      841 cacgtaccac acgttggcga cgtccaccgc caccaaagcc accgccaaac atttcgctga
      901 agatgtcttc aaaaccaccg aaaccaccgc cgccaaagcc accgaatccg cctgcaccgg
      961 caccgccttg ttcaaaggcg gcgtgaccgt attggtcgta catcgctttt ttctcgctgt
     1021 cggtcagcac gtcataggct tcggtaatct ctttaaattt ctcttccgac tctttactgc
     1081 cttggttttt atccgggtga tgctttgccg ccaaacgttt atatgctcgt ttgatgtctt
     1141 tctcgcttgc ccctttagac agtccaagga cttcgtaata atctttcttt gccattgttg
     1201 ttccgtttgt aaatttttct gtgtttttga ccgcttgccc tcgccccttg tgggagaggg
     1261 gtaggatggt taattccccc tctctctgaa aaatatttct gaacgaaata tttttcggtc
     1321 tctctcccac aaggggagag agttttatat aattaaatgg gatctagcca agatggctca
     1381 agatgacaca agcgtggacg cttgcgctat caaaagccta acaatgcgga ttcaccgcaa
     1441 tataaatttc tactttgccg tctgctaaat gtttttcaaa atctttggtg taagctcgtt
     1501 tgagcaaacc ttgtttggaa cggttttcaa tcacagacca agtgtctgcc aaaagagctt
     1561 cggtagttgg aatttgctca aaccaaacaa aatcgtccat taaaatcgcg gtattgctgt
     1621 tttccgtttc tgtggcaatg accgtttcgc cacttttatg atcgtatatt ttataaaacg
     1681 gtggctgtac attttccgtt gtagggtaga gttttagcat ttgtaaaatt tcctttgaaa
     1741 agcaccgctt gcacagcccc aaaggggctg aattatgtgt aacctagcac ggctcgtgct
     1801 aggtggtata tatctacaca aaaggggcga aagatatttc gcccctacaa gttacgcaag
     1861 attatttatt atctttcact tcttcaaatt ccgcatcgac cacgccgtca tctttttgag
     1921 attgttgtgg ttgctcgccg ccttgtgctt ttgcttgtgc tgcttgcatt aacggttctg
     1981 aagctttgat taacgcttcg attttcgctt cgatctcggc tttatcttcg cctttagctg
     2041 cggtttctaa ctcagaaata gccgcttcga tcttcgcttt atcgtctgcg tttaacgcat
     2101 cgcctgcttc ggtaatttgc ttacgggttg cgtgagcaat accgtctgct tggttacgag
     2161 cttgaactaa ctcttcaaat tttttgtctg cttccgcatt cgcttctgca tcacgcacca
     2221 tttgttcaac ttcagcatcg cttaagcctg aagatgcttg aattttgatt tgttgctctt
     2281 tgccagtgtt tttatctttt gcagagacat tgataatacc gttagcatca atatcaaagg
     2341 ttacttcaat ttgtggcata ccgcgtggag cagggttaat accttcaagg ttgaattgac
     2401 ctaaagattt attatccgct gcacgtttac gctcaccttg taacacgtgg atagttaccg
     2461 cagattggtt atcttccgct gttgagaaca cttgtgattt cttggttgga atcgtagtgt
     2521 ttttctcaat taaggtagtc attacaccac ccattgtttc gataccaagt gaaagcggag
     2581 taacgtctaa taacaatacg tctgttaccg aaccacttaa cacaccgcct tgaaccgccg
     2641 caccgattgc cacagcttcg tccgggttaa cgtctttacg cggttctttg ccaaagaagt
     2701 ctgccacttt tttctgtact aacggcatac gggtttgacc accgactaaa atcacatcgt
     2761 tgatgtcgcc tactgataaa ccggcatcag ctaaagcggt ttttaacggc tctaacgaac
     2821 ggtttactaa atcttcaact aaagattcca atttcgcacg ggttacttta atgtttaagt
     2881 gtttcgggcc ggttgcatct gccgtgatgt atggaaggtt cacttcagtt tcttgtgcag
     2941 aagaaagttc gattttcgct ttttccgctg cttctttcac acgttgcatt gccattgggt
     3001 cgttgcgtaa atcaacgcct tgttcttttt ggaattcttc cactaagtag ttgattacac
     3061 ggttatcgaa gtcttcacca cctaggtgag tatcgccgtt ggttgcacgc acttcaaaag
     3121 tttgctcgcc gtcaaagtta tcaatttcga taatcgaaat atcgaatgta ccaccaccta
     3181 agtcgtaaac agcaatcgtt tggttctctt tcttagaatc taaaccataa gcaagcgctg
     3241 ccgctgttgg ttcgttgatg atacgtttta catctaaacc tgcaatacga ccggcatctt
     3301 tagttgcctg acgttgtgca tcgttaaagt atgcaggcac agtaataacc gcttcggtta
     3361 ccggttcgcc caagaaatct tctgcggttt tcttcatttt tttcaagatt tcagcagaga
     3421 tttgcggcgg tgctaattta tcgcctttta cggttaccca cgcatcgccg ttatctgctt
     3481 tgctgatttc aaacggcata atttcaatat cacgttgcac ttcagcatca gtaaaacgac
     3541 ggccgattaa acgcttgatt gcaaataaag tatttttcgg gttagtaatt gcctgacgtt
     3601 ttgccggctg accaactaag gtttctttat cggtgtaagc gatgatagat ggcgttgttc
     3661 ttgccccttc cgcattttca attacacgag gtttgtcgcc gtccattacc gcaacacaag
     3721 aatttgttgt acctaagtca ataccaatga ttttacccat ttttatgatt cctataataa
     3781 taaattcagt ttgaataaga aacgggattg catttcccgt tcagacgaaa tcataaatag
     3841 ggacgaaaat gcactgttca agggcaaaaa taaaaaaatt gcaaaatttt tggaaaaagt
     3901 aaccgcttgt atgtggggga gatttggcag aatatttcac ggagaaaata cctcctacct
     3961 tgcatacccc tcaatagctt tttatactta cggcaactca aacagagaag gatataatat
     4021 gaaactgatt actaccatta tccaaccttc aaaattagac caagtgcaag atgcactttc
     4081 agaaatcggt attcaaggaa tgactattac cgaagtaaaa ggctttggac gccaatatgg
     4141 gcataccgaa caagagctta gccaaatgag ccgcattgac tttcgtccta aaattaaggt
     4201 ggagatattg gtttctaatt accaaaccga tgaagtgatc gatgccattc tcaatgcatc
     4261 ctattctggg caagtgggtg atggcaaaat ttatgtagcg aatattgaac aagttatccg
     4321 cacccgtacc ggcgaaactg acgatgaagc agtctagcca ttgctagcag agaagctttt
     4381 tattatcgtg ttttagagtt atattttcaa ccttactaaa gcaaattttc taatttggtg
     4441 tgaattaaat g
//
LOCUS       DANLRK010000180         4448 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.180, whole genome shotgun sequence.
ACCESSION   DANLRK010000180 DANLRK010000000
VERSION     DANLRK010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4448
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.180"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            <1..2063
                     /locus_tag="PXA33_002258"
     CDS             <1..2063
                     /locus_tag="PXA33_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein"
                     /protein_id="HDL5121556.1"
                     /translation="IIKIPNLKGFLPHCEIRRRPLVGKTFIAQKRPNQIIGWGLRPSA
                     VGSRAYAQKHQLPYIALEDGFLRSLGLGVKGYPPFSLVYDDIGIYYDTTCPSRLEKLL
                     LEKPLQDQEQIKLAERALSQILTYGLSKYNHALDFSEIPSAKEKIVLVIDQTFGDMAV
                     KYGQASEVDFNKMLETAISENPEAQIWVKTHPDVLSGKKKGYLTDLPQYTDRVRIITE
                     DVNPASLIKLCEKVYCVTSQMGFEALLLGKKVVTFGVPWFAGWGVTDDRNEAVQLLVQ
                     QNRRKDRSLLQLFYAAYFDYSRYINPNTGEKGSIFDVIDYLYKAKRLNQRLSGNLYCI
                     GMSLWKQAVIKPFFNLPQCRLHFVRSLNSLKKRPLADNAKILVWSNGKADVLQFAQQN
                     NIPLLRMEDGFIRSVGLGANLVAPLSLVVDDLGIYFNAENTSRLEQILLSAEHFSETD
                     LSYAETLRQKLVASHIGKYNVGGGDVKFNAQGKKIILVPGQVEDDASIKSGSPQIKRN
                     LDLLKKVRELNPSAYIIYKPHPDVVSGNRVGQIPTAETRRFADQMVDNANILDCILIA
                     DEVHTMTSLAGFEALLRGKKVHCYGLPFYAGWGLTEDRLKSARRGKSLTLSQLIAATL
                     IYYPLYVDPISRQFIDANQAIHILSQQKRNLTNQRIKRSWVSKQYGKLQHLYRAFKYA
                     SHTK"
     gene            2086..3318
                     /locus_tag="PXA33_002259"
     CDS             2086..3318
                     /locus_tag="PXA33_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis protein"
                     /protein_id="HDL5121557.1"
                     /translation="MEHYLDELIINSQRILLLQGPIGSFFTEFARWLESEQKEVFKLN
                     FNGGDEYFYPNNSPNTFPYYDSLDNFEAYLIQFCQTHQIDNILCFGDNRYYHKVAKNV
                     CLKLKLVFWAFEEGYFRPEYITLEKWGVNAFSPIPKNHTFFLPYAELPVVSEPEKVSK
                     GFRCMAGKAIPYYWNAYKKRKAYAKYQHHRFLNLKYYLNLWTRSGIKRICYWLHDKGF
                     AKRVEKGEFGEFFIVPLQVYDDTQVRIHSEQKSVERFLREVLNSFAKFAPAHLNLIIK
                     HHPMDRGFIDYKMVIYEFLEQYPQLNGRVFYIHDVPLPVFLRYGKGMVTLNSTSGLSA
                     LLHNMPVKTLGRANYDFEGLTDKQPLDTFWANPQPPNSEVFKGYRQFHLHKTHINGNF
                     YHKSILRYPYNWENEAHR"
     gene            3399..>4448
                     /locus_tag="PXA33_002260"
     CDS             3399..>4448
                     /locus_tag="PXA33_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL5121558.1"
                     /translation="MAIYAIWNNKGGVGKSYLTFQIACEYAKQYPAKKVLVVDLCPQA
                     NSSSMLLGGIEKGEQELAKIHSQVNKNTISGYISERLVSPYRSINSATKYVTQVSTIN
                     EYVPKNVYLVVGDEELEIQSSGILHASQAVHVPDSWRLVHLWVRDLVSDIQNAWDNDD
                     NVVFIDCNPSFTIYTELALAASDRLIIPFSADGSSKRAVKSVLSLVYGVKRHSGDVSS
                     HFYTESQKSKLALPQIYMYVGNRLTINAKASATAFKTVVEEIGDEIYNIWKTNANLFC
                     IHPAGVGTPTNKSSFRKMFQYEVRDANTASVVSGALGIPISRLSSGKRDVLGKNVMVN
                     QSQLDAQVPNIQNLVQ"
BASE COUNT         1434 a          704 c          902 g         1408 t
ORIGIN      
        1 aaattataaa aatccctaat ttaaaaggtt ttttacctca ttgtgaaatt aggcgaagac
       61 ctcttgtggg aaaaaccttt attgctcaaa aaagaccgaa ccaaatcatc ggctgggggc
      121 ttcgtccttc agcggtggga tcaagagctt atgcacaaaa acatcaactt ccctatattg
      181 cattagaaga cggcttttta cgctcactag ggctgggtgt aaaaggctat ccgccatttt
      241 ctttagttta tgatgatata ggtatttatt acgataccac ctgtccttct cgtttagaaa
      301 agttattgct tgaaaaacct ttacaggatc aagagcaaat caagcttgca gagagagcat
      361 tatctcaaat cttaacttac ggactttcta aatataatca cgcactagat tttagtgaaa
      421 tcccctctgc caaagaaaaa atcgtgttag ttattgacca aacctttggt gatatggcgg
      481 tgaaatatgg tcaagcgagt gaagtggatt tcaataaaat gttggaaacg gcaatcagtg
      541 agaatccaga ggctcaaatt tgggtaaaaa ctcaccccga tgtattaagt ggcaagaaga
      601 aaggctattt gaccgattta cctcaatata ctgatagggt cagaattatt actgaagatg
      661 tgaatccggc aagtttgatt aaattgtgtg agaaagtcta ttgcgtaact tcgcaaatgg
      721 ggtttgaggc attattatta ggtaaaaaag tggttacctt tggtgtaccg tggtttgccg
      781 gttggggggt aacagatgat cgtaacgaag cggttcaact attagttcaa caaaatagac
      841 ggaaagatcg ttcattactg caactttttt atgctgcgta ttttgattac agccgttata
      901 ttaatccgaa taccggtgag aaaggctcta tttttgatgt catcgattat ctatataaag
      961 ccaaaagatt aaatcagcgc ttaagtggga atctatactg tattggtatg tcgctatgga
     1021 aacaggcggt aattaagccg ttttttaatt tgccacaatg tcgattacat tttgttcgtt
     1081 cgctaaattc tttaaaaaaa cgaccgcttg cagataatgc aaaaatttta gtttggagca
     1141 atggcaaagc ggatgtcttg caatttgcac agcagaataa tattccattg ttaagaatgg
     1201 aagatggatt cattcgctct gtcggactag gcgctaattt agttgcaccg ctttctttag
     1261 tagtcgatga tttaggcatt tattttaatg cagagaacac ttcacgatta gaacaaattt
     1321 tattgagtgc agagcatttt tctgaaactg atttgtctta tgctgaaaca ttaaggcaga
     1381 aattggtggc tagccatatt ggaaaatata atgtgggcgg tggcgatgta aaatttaatg
     1441 ctcaaggtaa aaaaataatt cttgtaccgg ggcaggtaga agatgacgct tcgatcaaat
     1501 caggttctcc acaaattaaa agaaatctgg atttactgaa aaaagtgagg gaattaaacc
     1561 ctagtgccta tattatttat aagcctcacc ctgatgtggt aagtggtaat cgagtcggtc
     1621 agattcctac ggcagaaaca cgcaggtttg cggatcagat ggtggataat gccaatattt
     1681 tagattgtat tttaattgca gatgaagtgc atacaatgac atctttagcc ggtttcgaag
     1741 cattattaag aggtaaaaaa gtgcattgct acggattacc tttttatgct ggttggggat
     1801 tgacggaaga tagattgaag tcggcacgca gaggtaaatc tttgacatta tctcagttaa
     1861 ttgccgcaac attgatatat taccctctgt atgttgatcc tataagcagg cagtttattg
     1921 atgccaatca agcgattcat atattgtcac aacagaaacg aaacctaacc aatcaaagga
     1981 ttaaacgttc ttgggtgagt aaacaatatg gaaaattaca gcatctttat cgtgcgttta
     2041 aatatgcttc ccacactaaa taagcgtaga gggtaggaaa ttaagatgga acattattta
     2101 gatgagctaa tcatcaatag ccagagaatt ttattgttac aaggcccgat aggctctttt
     2161 ttcactgagt ttgcccgttg gttagaaagt gagcaaaaag aggtcttcaa gttaaatttt
     2221 aatggcggag atgaatattt ctatccaaat aatagcccga atacttttcc ttactatgat
     2281 agcttagata attttgaagc ctatttaatt cagttttgcc aaactcatca aatagataat
     2341 atcttatgtt ttggcgataa tcgttattat cataaagtgg ctaaaaacgt ttgcttgaag
     2401 ttaaagttgg tcttttgggc gtttgaagaa gggtacttta ggccagaata tattacgttg
     2461 gaaaaatggg gtgtaaatgc tttttctcca atccctaaaa atcacacttt ctttttgcct
     2521 tatgccgagt taccagtagt cagtgaacca gaaaaagtat ctaaaggctt tagatgtatg
     2581 gcgggaaaag caatcccgta ctattggaat gcctataaaa aacgtaaagc atacgcaaaa
     2641 tatcagcatc atcgttttct gaatctcaaa tactacctta atttatggac aaggtcggga
     2701 attaagcgaa tatgttattg gttacatgat aaaggctttg ccaagcgggt agaaaaagga
     2761 gagtttggtg aatttttcat tgtgcctcta caagtttatg atgacactca agtgaggatt
     2821 catagtgaac aaaaaagtgt agagcgtttt ttaagggaag ttctgaattc ctttgccaaa
     2881 tttgcaccgg cacatctcaa tttaattatt aagcatcacc cgatggatag aggatttata
     2941 gattataaaa tggtgattta tgaattctta gagcaatatc cccaattaaa cggtagagtt
     3001 ttttatattc acgatgtgcc gttacctgtc tttttacgtt atgggaaagg aatggtgact
     3061 ttaaattcaa ccagtgggct ttctgcattg ctccataata tgccggtcaa aaccttaggt
     3121 agagccaatt atgattttga gggattaacc gataaacagc cattagatac tttttgggct
     3181 aatcctcagc cacctaattc ggaagtattt aaaggctaca gacaatttca tttacataaa
     3241 acgcatatta acggtaattt ttaccataaa tcgattttgc gttatcctta taattgggaa
     3301 aatgaagcac acaggtgatg gaaaatatta aaataagtaa tatcttatag ctaaattatt
     3361 gtgttattat tacacctatt gttaaaagag ggggcattat ggctatttat gcaatatgga
     3421 ataataaagg tggggttggt aaaagttatt taacttttca aatcgcttgt gagtatgcaa
     3481 aacaataccc agcaaaaaaa gttctcgttg ttgatttatg tcctcaagca aattcatcat
     3541 ctatgctatt aggcggcatt gaaaaaggtg agcaagagtt agcaaaaatt cattctcaag
     3601 taaacaagaa tactatttca ggatatattt cagaaagatt agtaagtcct taccgttcta
     3661 ttaatagtgc aacgaaatat gtaactcaag ttagcactat aaatgaatat gtaccaaaaa
     3721 atgtttatct tgttgttggt gatgaggaat tagaaatcca atcatctggt attttacatg
     3781 cttcccaagc tgttcatgtt cctgattctt ggcggctagt gcatctttgg gtgcgtgatt
     3841 tagtttctga tatacagaac gcttgggata atgatgataa cgttgtattt attgattgca
     3901 atccaagttt cactatttat actgaattag cgttggctgc ttcagataga ttgatcatcc
     3961 ctttttccgc tgatggttca tcaaaacgag ctgttaagtc tgtattatct ctagtatatg
     4021 gggttaaaag gcactctggg gatgtcagct ctcattttta tacagaatct caaaagagta
     4081 agttagcatt acctcaaatt tatatgtatg taggtaatag attaacgata aatgccaaag
     4141 catcagcaac cgcatttaaa acagttgtag aggaaatagg tgatgaaatc tataatatat
     4201 ggaaaactaa tgctaatttg ttctgtattc acccagcagg tgttggtact cctacaaata
     4261 aatcaagctt cagaaaaatg tttcagtatg aggtgagaga tgcaaataca gcatctgttg
     4321 tttcaggtgc tttaggtatt ccaattagtc gcttatcatc aggaaaaaga gatgttttag
     4381 gtaaaaatgt aatggtgaat cagtcacaat tagatgctca agttcctaac attcaaaatc
     4441 ttgttcag
//
LOCUS       DANLRK010000181         4436 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.181, whole genome shotgun sequence.
ACCESSION   DANLRK010000181 DANLRK010000000
VERSION     DANLRK010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4436
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.181"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(24..1289)
                     /locus_tag="PXA33_002261"
     CDS             complement(24..1289)
                     /locus_tag="PXA33_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis protein HemY"
                     /protein_id="HDL5121559.1"
                     /translation="MFRTLFLMLLLLVGLISGPYLAGNQGYVRIETATTVIEMSIVML
                     VVFFVLAMAVVYLLEWIVTKICRMSNGAYNWFSTRKHKKAQQETLEGLMRMSEGNYSK
                     AEKLFSKNAKHADEPVLNLIKAAEAAQQNGDDLAANKYLIKATEIAGPNNVAVELVRT
                     RILLQQGKLPAARTAIDSLLELAPHSDEVLRLAIQIYKESRAYVALDRLLVEIGQRNF
                     LTAEEYAELEYFVDDGLQDERLQEEGQEGLLSWWENQPNRRRKSVYSRVGVIKRLIDS
                     DDHQSAQEIALETLKKFEDEQLQGLFVQLTRLQIDSDSKLLKVLAKRADKANVKYTDD
                     YARALGYIYTRDGQYQKAKSQFELLLAHDECVADDRIMALHVAEQMGDTLMANRIREE
                     NLKQVNMATQSKVNEPLALPQTATESNSL"
     gene            complement(1308..2618)
                     /locus_tag="PXA33_002262"
     CDS             complement(1308..2618)
                     /locus_tag="PXA33_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HDL5121560.1"
                     /translation="MSRSKKVTEHLEHSAEKVVEEAQQAVDSAGSFAKKEIEEDVVNE
                     AIEKDIQTEMQKAPKEQVVVQKSGGKGLALVALLVALAVGGAGHYMANKKFAEVESQI
                     QAVSAKANQPTTQTMVEMPNFENEKAQIAELVTEYQKALERIKALETAQNGYTQQISS
                     LQLQLQKLNNTSGSDNTFWLLSEADFLLNNAARKVVLDNDIETAKNLLLEADQVLSKA
                     PNTIHIREAIKTDLNTLTALHQIDQNALMQRVANLANKLDDLPILESEQASNSKQDGQ
                     VSDSLADWEKNLEKSAASFLDHFIRVSKRNAADEKAFVAPNQEIYLRENIRLRLQIAI
                     LAVPRQQSELYKKSLQTVGSWVRSYFETSNENVQHFLKELDELAEQTIYVDAPTSLQS
                     LKVIAQQLNKTPEKIEKVEIKAEKELEQAEPVKAEQDAMPLSAQ"
     gene            complement(2630..3394)
                     /locus_tag="PXA33_002263"
     CDS             complement(2630..3394)
                     /locus_tag="PXA33_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HDL5121561.1"
                     /translation="MNVLVTRLDSRGQELVDMLNEQQIFAIHQPLFTIEAGKELPLLP
                     SAISRLKSDDYVFVVSPNAVDYAAKTLVDTGFHFRSDLKYFAVGQRTAKYLTEKAEQA
                     VIYPIEFENSEGVLALPEMQNLTDKTILILRADSGRELLAETAVLRGATIQYLECYRR
                     EPVTDDIPEKISLCKRLGVDTIVITSSEILRSLYEQAKADCRAWLFECDLVVVSRRIA
                     KIAKQMGWQEDKIRVSDKADNVTLMNTLLFKVGKSN"
     gene            complement(3394..4329)
                     /gene="hemC"
                     /locus_tag="PXA33_002264"
     CDS             complement(3394..4329)
                     /gene="hemC"
                     /locus_tag="PXA33_002264"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HDL5121562.1"
                     /translation="MKDILRIATRQSPLALWQANFVKSELEKHFPQLSVELVTMVTKG
                     DIILDTPLAKIGGKGLFVKELELALLENRADIAVHSMKDVPMSFPEGLGLAVICERED
                     PRDAFVSNNYHSLEDLPKGAVVGTSSLRRQCQLMAKYPHLEVKSLRGNVGTRLSKLDN
                     GEYDAIILASAGLIRLGLQERIRSYISVEQSLPAAGQGAVGIETRANDERILNYIAKL
                     NHSSTACCVMAERAMNTRLQGGCQVPIGGYATLEGDELTLNALVGSLDGSQIIRASAK
                     GHKQEAEQLGILVAEQLLAQGADKILAAVYNENVQ"
BASE COUNT         1243 a         1025 c          714 g         1454 t
ORIGIN      
        1 caagcctgct atttttttaa ttattataat gagttagact cagtggctgt ttgcggtaag
       61 gctaatggct cattaacttt agactgagta gccatattca cctgtttgag attttcctca
      121 cgaatacggt ttgccattaa tgtatccccc atctgctctg ccacgtgcag tgccataata
      181 cgatcatcag caacacattc atcatgagcc aataacaatt caaactgaga cttcgctttt
      241 tggtattgcc catctcgtgt ataaatatag ccaagcgctc tagcataatc atcggtatat
      301 ttaacattcg ctttatctgc ccgttttgct aatactttta gtagcttact gtcagaatca
      361 atttgtaaac gggttaattg cacaaataaa ccttgtaatt gctcatcttc aaatttcttc
      421 aacgtttcta aagcaatttc ttgtgcagat tgatgatcgt cactatcaat taaacgcttg
      481 atgacaccga cacgactata caccgattta cgacgacgat ttggttggtt ttcccaccaa
      541 gaaagcaagc cttcctgccc ttcttcctgt aaacgctcat cttgtaaacc atcatcaacg
      601 aaatactcta actcagcata ctcttctgca gttaagaaat tacgttggcc gatttcaact
      661 aataatctat ctaacgcaac ataagctctg gattctttat aaatttgaat cgctaagcgt
      721 aatacctcat cgctatgagg tgcaagctct agtaggctat caatcgcagt tcgggcagcc
      781 ggtaattttc cttgttgtaa taaaattcgg gttcgtacca actccactgc aacattattc
      841 ggtccagcta tttcagttgc cttaattaaa tatttatttg cagctaaatc atcaccattt
      901 tgctgagcag cttccgccgc tttaattaaa ttcagtacag gctcatctgc atgttttgca
      961 tttttactaa agagtttttc cgcttttgag taattccctt cactcatacg cattaaacct
     1021 tccagtgttt cttgctgagc ttttttatgc ttacgagttg agaaccagtt ataagctcca
     1081 ttactcatac ggcatatttt agtgacaatc cattcaagta aataaactac cgccattgcc
     1141 aacacaaaaa aaaccacaag cattacaatg ctcatttcaa tcacggtagt tgctgtttca
     1201 attctgacat aaccttgatt tccggctaaa taaggcccag agattagtcc aactaataat
     1261 aaaagcatca aaaagagcgt tctaaacata atctattctc ctttttatta ttgtgctgaa
     1321 agaggcatcg catcttgctc tgcttttaca ggctctgctt gctctaattc tttttcagct
     1381 tttatttcga ctttctctat tttttcaggg gttttattaa gttgctgagc aatcaccttt
     1441 aagctttgta atgaagttgg agcatccaca taaatagttt gttctgccaa ctcatctagc
     1501 tctttcaaga aatgttgcac attttcattt gaagtttcaa aataacttct tacccaagag
     1561 cctaccgttt gtaacgattt tttataaagt tcgctttgct ggcgaggaac agctaaaatc
     1621 gcaatttgta aacgcaagcg aatgttttca cgcaaataaa tttcttgatt cggtgcaaca
     1681 aaggctttct catctgctgc attacgtttc gatacacgaa taaaatgatc taaaaatgaa
     1741 gcagcacttt tttctaaatt tttctcccaa tccgcaagag aatcacttac ttgcccatct
     1801 tgtttgctat ttgaagcttg ttcactttcc aaaatcggca aatcatctaa tttattcgct
     1861 aaatttgcga cacgctgcat taaggcattt tgatcgatct gatgcaatgc ggttaatgta
     1921 tttaaatcag ttttgattgc ctcacgaatg tgaatcgtat tcggtgcttt tgataaaact
     1981 tgatctgcct ctaacaataa atttttcgct gtttcaatgt cattatctaa caccacttta
     2041 cgagccgcat tattcaatag gaaatctgcc tcagataata gccagaatgt gttatccgaa
     2101 ccagaggtat tatttagctt ttgcagttga agctgcaaac tactaatttg ctgggtataa
     2161 ccattttggg ctgtttctaa agctttaatc cgttctaaag ctttttgata ttccgtgacc
     2221 aattcggcaa tttgtgcttt ttcattttcg aaatttggca tctcaaccat agtttgagta
     2281 gtaggctgat ttgctttagc agatactgcc tgaatttgag attcaacctc tgcaaacttt
     2341 ttatttgcca tataatgacc agctccgcca accgctaaag ccactaatag tgcaactaat
     2401 gccaagccct ttccgcccga tttttgaaca accacttgtt cttttggcgc tttttgcatt
     2461 tctgtttgaa tatctttctc tatagcttcg ttcactacat cttcctcaat ctcttttttt
     2521 gcaaaagatc ccgctgaatc gaccgcttgt tgtgcctctt ctaccacctt ttcagctgaa
     2581 tgctctaaat gttcagttac ttttttagat cttgacatac tcgccacctc tagttagatt
     2641 tgcccacttt aaataataat gtattcatca aagttacatt atcggcttta tccgataccc
     2701 taatcttatc ttcttgccag cccatttgtt tagctatctt tgcaatccgc cggctcacta
     2761 caaccagatc acattcaaat aaccatgctc tacaatctgc tttcgcttgt tcataaagtg
     2821 atcttaaaat ttcactactg gtgatcacaa tagtatctac tcccaagcgt ttacataagc
     2881 tgattttttc gggaatatca tcggtaaccg gctctctacg ataacactcc aaatattgaa
     2941 tagttgcccc acgcaagact gcagtttcag caagtagctc acgcccagaa tcggctctta
     3001 gaattaaaat agttttatca gttagattct gcatttcagg taatgccaaa accccctcgc
     3061 tattttcaaa ttctatcgga taaatgaccg cttgttcagc tttttctgtt aaatatttcg
     3121 cagtccgttg ccctaccgca aaatatttca aatctgaacg aaaatggaac ccggtatcta
     3181 ctaatgtttt tgcagcatag tccactgcat tcggcgaaac aacaaataca taatcgtctg
     3241 attttagccg agaaatagcc gaaggaagca aaggtaattc tttaccggct tcaatcgtaa
     3301 atagcggttg atgaatagca aaaatttgct gttcattcaa catatctacc aattcttgcc
     3361 cccgactatc caggcgtgtg accaacacat tcattattgt acgttttcgt tataaaccgc
     3421 agctaaaatt ttatctgcac cttgagcaag caactgttcc gccaccaaaa tgcctaattg
     3481 ctccgcctct tgcttatgtc cttttgccga agcccgaata atttgtgagc catctaacga
     3541 tccaactaac gcattaagcg ttaattcatc gccctctaat gtagcataac caccaatcgg
     3601 cacttggcaa ccgccttgca aacgggtatt cattgcacgt tctgccatca cgcaacaagc
     3661 ggtcgaacta tggtttaatt ttgcaatgta gttcaaaatc cgttcatcat tggctcgagt
     3721 ctcaatccct actgctcctt gaccagctgc gggtaatgat tgttccacag aaatatagct
     3781 acggattcgc tcttgcaagc ctaaacgaat taagccggca gaggctaaaa taatcgcatc
     3841 atactcgcca ttatctaatt tgcttaagcg agtgccgaca ttgcctcgca atgatttaac
     3901 ctctaaatgc ggatatttcg ccattaattg gcactgacgg cgtaagctcg atgtaccgac
     3961 cacagcacct ttcggcaaat cttctaacga atggtagttg ttcgacacaa acgcatcacg
     4021 gggatcttca cgttcacaaa tcaccgcaag ccccaagcct tcgggaaagc tcatcggcac
     4081 atctttcatt gaatgcaccg caatatcagc tcgattttct aaaagggcta attctaactc
     4141 tttcacaaac aagccttttc cgccaatttt ggctaatggg gtgtctaaaa taatatcgcc
     4201 tttcgttacc atcgtaacca gttcgacaga taattgcgga aaatgttttt ctaattcgct
     4261 ttttacaaaa tttgcctgcc ataaggctaa cgggctttga cgggttgcaa tgcgtaaaat
     4321 atctttcata aattctaaaa ttaaaacatt gagttaatga attatcctac cactttttag
     4381 ctgaactgcc aaaaataatc gtggatttcg ctttgcattt aatcaaaaat ccgact
//
LOCUS       DANLRK010000182         4355 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.182, whole genome shotgun sequence.
ACCESSION   DANLRK010000182 DANLRK010000000
VERSION     DANLRK010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4355
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.182"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(81..698)
                     /locus_tag="PXA33_002265"
     CDS             complement(81..698)
                     /locus_tag="PXA33_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3164 family protein"
                     /protein_id="HDL5121563.1"
                     /translation="MSKVTIGNEIYWKDADGNLKPEALVKEIDKERDELVRQFVSKAQ
                     ALSKALGDFKQSVFDDVGAFVSLSADKYGVKIGGAKGNITLFTYDGEYKLQLAVQENI
                     RFDERIHAAKALIDECLHDWSEGAKPELKALIDNAFEVDKEGNLSTAKILSLRRVEID
                     DARWNQAMTAISDSVQVIGSKGYIRFYKRDENGKYQPISLDMAGV"
     gene            complement(711..902)
                     /locus_tag="PXA33_002266"
     CDS             complement(711..902)
                     /locus_tag="PXA33_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121564.1"
                     /translation="MKKRRMIYALYVGEENVADGTAEELAERLGVEAKTVRFMSSPAH
                     NKRVKYNGKAIRTVKVGLE"
     gene            complement(905..1225)
                     /locus_tag="PXA33_002267"
     CDS             complement(905..1225)
                     /locus_tag="PXA33_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121565.1"
                     /translation="MQKVYSEKAGEMMNPLNAFIHDSLVKLEIATLECEAMELEVERV
                     EFEAKSPPRLVLRDNCKTQDLVKNGLASLFGMKTKGGRRYDSYQMQVRGVKCVWESER
                     VTKV"
     gene            complement(1236..2117)
                     /locus_tag="PXA33_002268"
     CDS             complement(1236..2117)
                     /locus_tag="PXA33_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005606441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL5121566.1"
                     /translation="MKNQELRALMDSKGYQQKQVAQLLGVSVATVSLYLKGDYNGNVA
                     EMDRKVDELIERDKAKVVEAKYNAAFVPTLAARRGMEVMQFAHIEGEINVIFGAAGLG
                     KTQMLKEYERRNSSATLIEVDPSCTPKVLLRKIAEAVGANARGVNNELLESIVNKLKG
                     SERLLMIDEAELLSTRSLEFIRRIHDLTSVGVVLAGMPRLLVNLKGKNNELAQLYSRV
                     GFACDLGNALSEEDLGLLAESALGTNEFNAPLIKACHGNARRLSKLMRGVVRSSEINQ
                     TPISKDLVEQYSKMLIS"
     gene            complement(2121..4058)
                     /locus_tag="PXA33_002269"
     CDS             complement(2121..4058)
                     /locus_tag="PXA33_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL5121567.1"
                     /translation="MKDWFSAKELAGISGLSKYPTNVTRQAKKEQWATRPLKGVKGGG
                     FEYHINSLPTEVQTEIRSRFMSAVVEAKPKKLPVVRAEVDVGNLTTKQREIADARMAL
                     VAYVLELEGSMSRIKAITYLCNLAKQGEMPPHLAELVAVANAKKTAKRTLSVRTLNGW
                     VVDYCKAENAEQRLKLLAPQVRQETKPEEIWWLSAFLGVYRQKNGICLTEAYREFEVE
                     WAYQYADNPLLLAQCPSLSQVQRAMNKLPLYVKEYGRRTGSHYKQLLSYVKRDWSVLR
                     ANDVWIGDGHSLKLKVAHPIHGKPFTPELTMIVDGAGRKVVGWSLALSENAFAVADAL
                     RHAISLHGVPAIYYSDNGGGEKNKFLDAEITGMLPRLGIRHETGIAGNPQGRGIIERL
                     NKTIGMTIARQFETGYATGADPETVRKTLYAVNSLANAKGELTPLQRKAQGKLPTWQQ
                     LIEVIQSVIDWYNNEHIHSEIRTTPARKYQQMLHAEDVVMLSPIELRDMSRPEFIRKP
                     DRGWVSWNNNHYFNLKLLDFDREEVVIGIDIHNAESVQVRTKDGRFICEAIWNGNTRE
                     AFPVAMVEQQRKERHKRRANLKQQQLDEINAELNPVLTIEQKNDFSLLATARKPKKEV
                     EPIFLTRAEKEDYEKKLAVGG"
     gene            complement(4073..4300)
                     /locus_tag="PXA33_002270"
     CDS             complement(4073..4300)
                     /locus_tag="PXA33_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL5121568.1"
                     /translation="MNKKKRAQDMHRADIRAELMKKGITFSQLGIENGLSKTTVRNAL
                     DKPYKNGEEIIAKALGKTPAEIWPSRYPDVA"
BASE COUNT         1008 a         1166 c          807 g         1374 t
ORIGIN      
        1 tattttctat tgaacaaacc ttgtctgtat taaccacaac aggtttatcc gtataattaa
       61 cattaaaagt aataaacatt ctaaaccccc gccatatcta aactaatcgg ctgatacttt
      121 ccgttttcat cacgtttata aaaacggata taacctttac taccgatcac ttgtacgcta
      181 tctgaaatcg ctgtcattgc ttgattccat cttgcatcat caatctcaac acggcggaga
      241 gagagaattt ttgctgtaga taaattacct tccttatcca cctcaaacgc attatcaatt
      301 aaggctttaa gctccggttt tgccccttcc gaccaatcgt gcaagcattc atcaatcaag
      361 gcttttgctg cgtgaatacg ctcatcaaag cgaatattct cttgcactgc taactgcaac
      421 ttatattcac cgtcataagt aaatagtgtg atattgcctt ttgcaccgcc aatttttact
      481 ccatatttgt cagccgatag gctgacaaaa gcccccacgt catcaaatac ggattgttta
      541 aaatcaccta acgctttgct taacgcttgt gctttgctta caaattggcg aactaattca
      601 tcacgttctt tgtcgatttc ttttactaat gcctccggct ttaaattgcc atcagcatct
      661 ttccagtaaa tttcattacc gattgttact ttactcataa tacttcctct ctattccaac
      721 ccgactttca cggtgcggat tgctttaccg ttatatttca ctcttttgtt gtgagcaggg
      781 gacgacataa accgcactgt tttcgcctcc acccccaacc gttctgctaa ctcttccgcc
      841 gtaccgtcag ccacattctc ttcgccaaca tacagggcat aaatcattcg ccgttttttc
      901 atttctacac cttcgtaaca cgttcagact cccacacgca tttcaccccc cgcacctgca
      961 tttgatagct gtcataccgt ctgccgcctt tggttttcat tccaaacagg cttgccaagc
     1021 cgttcttgac taaatcctgt gttttgcagt tatcccgtaa gaccaagcgg ggtgggcttt
     1081 tcgcttcaaa ctccactcgt tcaacttcca actccatcgc ctcacattcc aaagtggcaa
     1141 tttctaactt cacaaggctg tcgtggataa acgcatttaa ggggttcatc atttcgcctg
     1201 ctttttcgct gtaaaccttt tgcataactt gctccttagc taatcaacat cttgctatat
     1261 tgctcaacca aatctttact aattggtgtt tggttaatct cgctagaccg caccacgcca
     1321 cgcattaact tacttaatcg gcgggcattg ccgtgacaag ctttaattag cggggcatta
     1381 aattcatttg tacctaacgc actttcggct aatagcccca aatcttcctc gctcaaggca
     1441 tttcccaaat cacaagcaaa acctactcgg ctataaagct gtgccagctc attattttta
     1501 cctttaagat taaccaacaa tctcggcata ccggctaaca ccacgcccac gctggttaaa
     1561 tcgtggattc ggcggataaa ctccagcgag cgggtcgaaa gtaattcagc ttcatcaatc
     1621 attagcaacc tttcagagcc ttttaacttg ttcacaatgc tttctaatag ttcgttattt
     1681 acccctctgg catttgcccc caccgcctcg gcaatcttgc ggagcaagac tttaggtgtg
     1741 caactcggat caacctcaat taatgttgca gagctgtttc tgcgttcgta ttctttgagc
     1801 atttgggttt tacctaagcc ggctgcccca aaaatcacat taatttcgcc ttcaatatgg
     1861 gcaaactgca ttacctccat tcctctgcgt gccgccaaag ttggcacaaa agcagcgtta
     1921 tatttcgctt ccaccacttt tgccttatca cgctcaatca gctcgtctac tttgcgatcc
     1981 atttcagcca cattgccgtt ataatcgcct ttgagataca gactgactgt ggcaacggat
     2041 acacctaaaa gctgtgccac ttgtttttgt tgatagcctt tgctatccat taaggctcta
     2101 agttcttggt ttttcatcgt ttagcctccc actgctaact ttttttcgta atcctctttt
     2161 tctgctcggg tcaaaaaaat cggttctact tcttttttcg gttttcttgc cgttgccagc
     2221 aagctaaaat cgtttttctg ctcaatggtt aaaaccgggt ttaattccgc attaatctca
     2281 tcaagctgtt gctgtttaag gttggctcgg cgtttatggc gttctttacg ctgctgttct
     2341 accatcgcca ccgggaatgc ctctcgggtg ttaccgttcc aaattgcctc gcaaataaac
     2401 ctgccgtctt tggtacgcac ctgcacgctc tcggcattgt gaatatcaat cccgatcacc
     2461 acttcttcac ggtcaaaatc cagtaatttc aagttaaaat agtggttgtt attccaactt
     2521 acccaacctc gatccggctt acggataaat tccggtcggc tcatatcccg caactcaata
     2581 gggcttaaca tcaccacatc ttctgcgtgc aacatctgct gatatttgcg tgccggcgtg
     2641 gtacggattt ccgagtgaat atgctcgttg ttgtaccagt caatcaccga ttggattact
     2701 tcaatcaact gttgccacgt tggcaatttg ccttgggctt tacgttgtag tggggttaat
     2761 tcccctttgg catttgccag tgagttcacc gcatacaggg ttttgcgaac ggtttccgga
     2821 tctgcaccgg ttgcatagcc ggtttcaaac tgtcgggcaa tcgtcatacc gatggtttta
     2881 tttaagcgtt caataatgcc tcgcccctgc ggattgcccg caatccctgt ttcgtgtcgg
     2941 ataccgaggc ggggcaacat ccccgtgatt tccgcatcca aaaatttatt cttttcacca
     3001 ccaccgttat ccgagtagta gattgccggc acaccgtgta ggctaatggc gtggcgtagt
     3061 gcatcagcca ccgcaaaggc gttctcactg agtgccagcg accagcccac cacttttcta
     3121 ccagcaccat cgacaatcat tgtcagctca ggggtaaacg gcttaccgtg tatcgggtgg
     3181 gctactttga gcttcagtga gtgaccgtca ccaatccata catcatttgc tcgaagtacc
     3241 gaccaatccc gcttcacata gctcagtaac tgcttgtaat gcgagccggt gcgtctgccg
     3301 tactctttca catacaacgg caacttattc atcgctcgct gtacttggct taggctgggg
     3361 cattgtgcca acagtagcgg gttgtctgca tactgatacg cccattccac ctcaaattct
     3421 cggtaggctt cggtgaggca aataccgttt ttctgccgat atacgcctaa aaaagcactt
     3481 agccaccaaa tttcttccgg cttggtctcc tgccgtacct gtggggcaag cagtttcaac
     3541 cgttgctcgg cgttctctgc cttgcagtaa tccaccaccc agccgtttaa ggttcgcacc
     3601 gagagtgtgc gttttgccgt ctttttagca tttgctacgg caaccagctc cgccaagtgc
     3661 ggtggcattt cgccttgttt ggcaaggttg caaaggtagg ttatcgcttt aatccggctc
     3721 attgagcctt ccaactccaa cacatacgcc accagtgcca ttcgtgcatc agcaatttct
     3781 cgctgtttgg tggtcaggtt tcccacatcc acctctgcac gcactaccgg taactttttc
     3841 ggcttggctt ccaccaccgc cgacataaag cgggagcgga tttcggtttg aacttcggtg
     3901 ggtaaagagt taatgtgata ttcaaaacca cctcctttaa cccctttcaa tggacgagtt
     3961 gcccattgtt cttttttggc ttgtctagtt acatttgttg ggtattttga taagccacta
     4021 atacctgcta attcttttgc actaaaccaa tctttcataa cagctatcct tgttatgcaa
     4081 catcagggta acgactaggc catatttcgg ctggcgtttt tcctaatgct tttgcgataa
     4141 tttcttcacc gtttttataa ggtttatcaa gagcatttct tactgttgtt ttggaaagcc
     4201 cattctcaat ccctaactga gaaaatgtta ttcctttctt catcaattca gctcgaatat
     4261 cagctctatg catatcttga gctcttttct ttttattcat tttgtgagat cctccacatt
     4321 tagtttattt gctacgcttt agcgtacttg attaa
//
LOCUS       DANLRK010000183         4268 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.183, whole genome shotgun sequence.
ACCESSION   DANLRK010000183 DANLRK010000000
VERSION     DANLRK010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4268
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.183"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(66..374)
                     /locus_tag="PXA33_002271"
     CDS             complement(66..374)
                     /locus_tag="PXA33_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HDL5121569.1"
                     /translation="MKKYIATMLLAFGLVHSSYAQEKTAQIETKQAEKTVVLHIPEMY
                     CQLCVYLVNKELRAVEGVISTKASMKERKATVVVKESVDNQALIQAVGNLKYTAKVIE
                     "
     gene            complement(376..744)
                     /locus_tag="PXA33_002272"
     CDS             complement(376..744)
                     /locus_tag="PXA33_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121570.1"
                     /translation="MNSSLKSYNHKFITMCVTAIVSAVTSTFCCIGPLVYLVFGISSP
                     WLMELNQLEFLQLPMLLISLAAFGYGFWLLNFSNKIICTKYFSRRTLVILYWVVSVVV
                     MFFLTYPYVLPYILEMWSGE"
     gene            complement(719..1825)
                     /locus_tag="PXA33_002273"
     CDS             complement(719..1825)
                     /locus_tag="PXA33_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005647785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HDL5121571.1"
                     /translation="MNKLIASLVASSCATAFAAPVQQNNFHTTTHTYEFTQSYDLVVP
                     QGSKGTTNLWIPLPSDTDYQTVKSIEFEGSYKNAYITENNAYGAKTLFATWDTDAPKR
                     DLKVKMVIQTQDREPMAQGALANYQMPEKITYSVDVLEYLKPTAHIKTDGIVKEYADK
                     IVGNEQNPLKKAELIHKWIVDNMERDNSVLGCGDGDVEKILTTKVLKGKCTDINSVFV
                     ALARASNIPAREVFGIRLGQAVKMGQYSKTAFGSSKDGVANESGGQHCRAEFYLAGFG
                     WVPVDSADVAKMRLAEKKDVDDADVQAVSKYLFGNWEMNWVGFNHGRDFDLYPQPELT
                     PLNNFGYPYAEVGGDPLNSFEAKEFGYEFISKEL"
     gene            complement(2065..3108)
                     /locus_tag="PXA33_002274"
     CDS             complement(2065..3108)
                     /locus_tag="PXA33_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL5121572.1"
                     /translation="MQPINIAIAGAGFSSRVFHIPFLKQDARFNIVKVYERTSNNAAE
                     WLPNAETVRDFNALLTPEVDLVLITTPNQTHYAMVKESLLAGKHVLVEKPLVATVAEA
                     EELAELAKRQGKVLYVYQNRRWDSHIATAKSLMEQNLLGELVDCEIRFDRYAKEKNVK
                     VWKESGELGTGLVYDLGVHLIDQALYLFGKPEAVYADVRYQHEGALVDDNFDIQLYYA
                     NGFKVVLKASRYAREPSPTFVLHGKLGSYLKQTPDNQEDLLKNGVQPIGKDWNIEEKE
                     NWGILHTEVEGNVVRQPYRNAEVSYQNLFDDLYQAIAHNAEPIIKLEDVIFVLKVIES
                     VFESAKLGQKIYL"
     gene            complement(3212..3496)
                     /locus_tag="PXA33_002275"
     CDS             complement(3212..3496)
                     /locus_tag="PXA33_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121573.1"
                     /translation="MRYWLISLSILLSGCVVPVTPTGQAGIPMPIIIGVSDPLDSKEV
                     HICTLKPFTQEYRSEHSSRGRAKLNVQKQCRANHSEMFCEEKDIVCKTYD"
     gene            complement(3526..3711)
                     /locus_tag="PXA33_002276"
     CDS             complement(3526..3711)
                     /locus_tag="PXA33_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HDL5121574.1"
                     /translation="MKCRQATELISQSCDEKLRLQQHMRLQLHLLVCPKCRNFNKNTK
                     KLGKILRAYCNDKDAPF"
     gene            complement(3708..4229)
                     /locus_tag="PXA33_002277"
     CDS             complement(3708..4229)
                     /locus_tag="PXA33_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL5121575.1"
                     /translation="MVKFATLQLADKDLAEDVVQDALTNAYKHSASFRREAALKTWIF
                     AILKNKILDLLRTRKRHIPVSSLCEEEESPSAFFDRHDHWLEHQDVTEWRGVAQSTYQ
                     KEFWAIFDICLNKLPAQQARVFMMREHLEMSSAEICQECEISLSNLNVLLHRARLRLQ
                     ECLSRNWFREESK"
BASE COUNT         1241 a          931 c          802 g         1294 t
ORIGIN      
        1 attagatggg gaaaaaagga aggctaaatt aaaaaagctc aaacaataat gtttgagctt
       61 tggttttatt caatgacttt tgcagtatat ttgaggtttc cgaccgcttg tatcagggct
      121 tggttatcca cactttcttt taccaccaca gtcgcttttc gctctttcat tgaggctttg
      181 gtggaaatca caccttctac tgctcgtagt tctttattga ctaaataaac gcagagctgg
      241 cagtacattt ccggaatatg gaggacgacg gttttctctg cttgtttcgt ttcaatttga
      301 gctgtttttt cttgtgcgta gcttgaatga accaaaccaa aagccaacag catcgttgcg
      361 atatattttt tcattttatt ctccgctcca catttccaaa atgtaaggca atacataagg
      421 gtaagttaaa aagaacatca ccaccacaga aactacccaa tacaaaatga ccagggttct
      481 gcgggagaag tatttggtgc agatgatttt gttggaaaaa ttcagcaacc agaagccata
      541 gccaaacgca gcgagcgaaa tcagcaacat cggcagttgt agaaactcca gttggtttag
      601 ctccatcaac caaggggaag agatgccaaa cactaaataa accagtggcc caatacaaca
      661 gaatgtggag gtcaccgccg acactatggc ggtcacgcac attgtgataa atttatggtt
      721 ataactcttt agagatgaat tcatagccaa attctttcgc ctcaaacgag tttaacgggt
      781 caccacctac ctcagcataa gggtagccaa agttattcag tggtgtaagt tccggttgtg
      841 gatataaatc gaaatcacgt ccgtgattga agcctaccca gttcatttcc cagttgccga
      901 ataagtattt cgagaccgct tgaacatcag catcatccac gtcttttttc tcggctaaac
      961 gcattttagc gacatcggca gaatctacag gcacccagcc gaagcctgct aaatagaact
     1021 ctgcacggca gtgctgcccg ccgctttcat tcgccacgcc atcttttgag ctaccaaatg
     1081 cggttttgga atattgcccc atttttaccg cttgccctaa acgaatacca aacacttcac
     1141 gagcaggaat gttagaggca cgagcaagtg ctacaaacac cgagttgatg tcggtacatt
     1201 taccttttag cactttggtg gtgagaattt tctccacatc gccatcgcca cagcctagca
     1261 ccgagttatc acgttccata ttatcaacaa tccacttgtg gataagttcc gcttttttca
     1321 acggattttg ctcgttaccc acaattttat cggcatattc tttcacaata ccgtctgttt
     1381 taatgtgagc cgttggtttt aaatactcca atacatcaac agaataagtg attttctccg
     1441 gcatttggta atttgccaac gccccttgag ccataggctc acgatcttgg gtttggatca
     1501 ccattttcac ttttagatca cgctttggtg catcagtatc ccaagtggca aacaaggttt
     1561 ttgcaccata agcgttattt tctgtgatat aggcgttttt gtaacttccc tcaaactcta
     1621 tcgattttac cgtttgataa tcggtatcag acggcaacgg aatccacaaa ttagttgttc
     1681 ctttcgagcc ttgtggcaca actaaatcat aagattgcgt aaattcgtaa gtgtgggtag
     1741 tggtgtggaa attgttctgt tgaacaggag cagcaaaagc cgtcgcacag cttgaagcca
     1801 ccaaagaggc aataagttta ttcattgaaa tgatccttat tagagtgaaa aaacagttaa
     1861 aacttaacat attaagtatt ctaactacgg attgaggttg catattagcc aatcgagatg
     1921 ggaaaagcaa tgggggagat gattttttag atagtgcaag cgtccacgct tgtactagaa
     1981 acaaagctaa aataatcaat aggcacaagc gtggacgctt gcgccatcag agagaataac
     2041 aagcggtcaa aaatcctcaa aattttacaa atagattttt tgccctaatt ttgcactttc
     2101 aaatacgctt tcgatcactt ttagcacaaa aatgacgtct tcgagtttga taattggctc
     2161 agcattgtga gcgatggctt ggtacaaatc atcaaacaag ttttgataac tgacttcggc
     2221 atttcgatag ggctggcgaa ccacattacc ttccacttca gtgtgtaaaa ttccccagtt
     2281 ctccttttct tcgatattcc aatctttgcc aataggttgt acgccatttt tcagcaaatc
     2341 ttcttgatta tcgggagttt gtttcagata agagcctaat tttccgtgta atacaaatgt
     2401 cgggcttggt tcacgagcat atcggctggc tttcagcacc actttaaaac cgttagcata
     2461 atagagctga atatcaaagt tatcatcaac caatgcccct tcgtgctgat aacgcacatc
     2521 agcatacacc gcctccggct tcccgaaaag atacaaagct tggtcaatta aatgtacgcc
     2581 taaatcatac actaaacctg taccaagctc gcctgattct ttccatactt taacattttt
     2641 ctctttcgca taacggtcaa aacgaatttc gcaatctacc aactctccca gtaaattttg
     2701 ctccattagc gattttgccg tcgcaatatg gctgtcccaa cggcggtttt ggtacacata
     2761 aagcaccttt ccttgccgct ttgcaagttc cgccaactct tccgcctcag ccactgttgc
     2821 cactaacggt ttttcaacta aaacgtgttt gcctgcaagc aatgactctt ttaccattgc
     2881 ataatgggtt tgattcggtg tggtaatcag cactaaatcc acttctggcg ttaaaagagc
     2941 gttgaaatcc cgcactgttt ctgcattcgg caaccattct gccgcattgt tagaggttcg
     3001 ttcatatacc tttacaatat taaaacgagc atcttgtttc aaaaacggga tatgaaaaac
     3061 tcggctggaa aagccggcac ctgcgatggc aatattgatg ggttgcatag acaatcctca
     3121 taaaattttg atgttatctg cttaaataaa caagcggtca aattttagga aaattttgca
     3181 aaaaacagat aaaatacgac cgcttgttta actaatcata ggttttgcaa acaatatctt
     3241 tttcttcaca aaacatttca ctgtggtttg cacggcattg cttttgtaca tttaatttgg
     3301 ctctgcctcg gctactatgt tcactacgat attcctgagt aaatggcttt agagtacaaa
     3361 tgtgaacctc tttggaatca agtggatctg agacaccaat tataatcggc atagggatac
     3421 cggcttgccc cgtaggagta accggcacga cacagccact taatagtatg cttaaactta
     3481 ttaaccaata acgcatattt ttctcctttt aaaatgtata ataaatcaga acggagcatc
     3541 tttatcgtta cagtaagctc ttaatatctt gcctaatttc ttggtatttt tattaaaatt
     3601 tcggcattta gggcatacca aaagatgcaa ctgcaaacgc atatgttgtt gcaaacgaag
     3661 tttctcatca caagactgtg aaataagttc tgttgcttgc ctgcatttca ttttgattcc
     3721 tctctaaacc aattgcgaga taaacactct tgtaaacgta aacgagcacg atgaagtaat
     3781 acatttaagt tagagagaga aatttcacat tcttggcaaa tttccgcact actcatctcc
     3841 aaatgttcac gcatcataaa tactcgtgct tgctgggcgg gtaatttatt taaacaaata
     3901 tcaaaaattg cccaaaattc tttttggtaa gttgattgag ctacccccct ccattcagtt
     3961 acatcttgat gttccaacca atgatcgtgg cgatcgaaaa aggcacttgg tgattcttct
     4021 tcctcacata gagaactgac tggaatatgt cgttttcgtg ttctgagtaa atctaaaatt
     4081 ttgttcttta aaatagcaaa aatccaagtt ttaagtgcag cttctcgccg aaaagaggct
     4141 gaatgcttat aggcattggt taaggcatcc tgtacaacat cctcagctaa atctttatcg
     4201 gctagttgta gggtggcaaa tttaaccatt tgctttctaa tctcttctat ctgtgtagaa
     4261 gatagact
//
LOCUS       DANLRK010000184         4259 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.184, whole genome shotgun sequence.
ACCESSION   DANLRK010000184 DANLRK010000000
VERSION     DANLRK010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4259
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.184"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            17..517
                     /locus_tag="PXA33_002278"
     CDS             17..517
                     /locus_tag="PXA33_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL5121576.1"
                     /translation="MKKSLFVLSLISFALVGCSSNSGESTSAVETTEANATWQSADSI
                     QTAPMPASMNQPIATQPTYQQPTYSAPQPISAAPAPMPVSSGSDSSQVETVGNCQVVR
                     DANNAPIYSQITKGCYTESHYTVGKSDTLYLIGYLTGTNANQIAQLNGLNTGSPLKVG
                     QVLRVR"
     gene            530..730
                     /locus_tag="PXA33_002279"
     CDS             530..730
                     /locus_tag="PXA33_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121577.1"
                     /translation="MNRTLLAFAISCAFLTACSSPTQPQVEDPNMLPAGTLQPIEGSG
                     ASQDSYSWPSDIQSTSMPDSMK"
     gene            749..2029
                     /locus_tag="PXA33_002280"
     CDS             749..2029
                     /locus_tag="PXA33_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HDL5121578.1"
                     /translation="MKKQFILLPLVSAVLAACSSNNPAPVVNASGNNELSPGVMQPVD
                     VSAGAINSSGGWQSDIQSAPMPSSMNTTPVQTMPQPVSVPQPVATVPQPITNPVITEQ
                     PPQPTTTTKIVKKTKTVEKKVDQNFEIPRDANNAPMYNQIQKGFYDGSTYTVRKGDTM
                     FLIAYIVGKDVKEIAALNNMSEPYQLSVGQKLKTGKSATETITVEEKVTVPVEPQITY
                     QQGANGTTYASGGNITGPVKAGTANADVTPVNNGIKATTATAPAVTASVGTVNATEDR
                     TASTIRAIDGTASGAVQSSNTVSAPASSIKWQWPTSGRVISGFSSAEGGNKGIDIAGN
                     KGQDVRAAAAGKVVYAGNALQGYGNLIIIKHTDDFLSAYAHNNTIEVDEQETVKAGQK
                     IATLGSTGTNTNKLHFEIRYKGKSVDPARYLPRK"
     gene            2270..2461
                     /locus_tag="PXA33_002281"
     CDS             2270..2461
                     /locus_tag="PXA33_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121579.1"
                     /translation="MDPTEPSHRIYILLSEDRFQAEVFSTKLSESSMLLEAVKGGYIS
                     KDGKVRLLKKAEGWKIYYE"
     gene            2454..3020
                     /locus_tag="PXA33_002282"
     CDS             2454..3020
                     /locus_tag="PXA33_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HDL5121580.1"
                     /translation="MSETKIRCDWCKSSEIYMKYHDEEWGKPEFDSLKLFEKICLEGQ
                     QAGLSWITVLKKREAYRQAFHQFNPEKIAKMGEEEIDLLMQNTSLIHHRAKLEAIIKN
                     AKAYITMQQNGEDFSRFIWSFVNYQPQINDVPTLEAVPAHTETSKAMSKALKKRGFVF
                     VGETTCYAFMQSMGLVNDHLNNCVFKHK"
     gene            complement(3067..3297)
                     /gene="acpP"
                     /locus_tag="PXA33_002283"
     CDS             complement(3067..3297)
                     /gene="acpP"
                     /locus_tag="PXA33_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005725021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDL5121581.1"
                     /translation="MSIEERVKKIIVDQLGAKAEDVKPEASFIEDLGADSLDTVELVM
                     ALEEEFDIEIPDEEAEKITTVQSAIDYVQNNQ"
     gene            complement(3484..4161)
                     /gene="rpe"
                     /locus_tag="PXA33_002284"
     CDS             complement(3484..4161)
                     /gene="rpe"
                     /locus_tag="PXA33_002284"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDL5121582.1"
                     /translation="MSKNPYLIAPSILSADLARLGDDVAEVLKAGADLIHFDVMDNHY
                     VPNLTFGPAICKALRDYGIQCPIDVHLMVKPVERLIPDFAKAGADYITFHPEATEHID
                     RNLQLIRDLGCKSGLVFNPATPLSYLDYVMDKVDVVLLMSVNPGFGGQSFIPSTLDKL
                     KEVRRRIDESGYNIRLEVDGGVKINNIADIAAAGADMFVAGSAIFDQPDYQAVIAQMR
                     DELRKVG"
BASE COUNT         1353 a          924 c          814 g         1168 t
ORIGIN      
        1 ttttaaggat ataaaaatga aaaaatcact ttttgtttta tctctgattt cttttgcttt
       61 agtgggttgt tcaagcaatt cgggtgaatc cacttctgcg gtagaaacaa cagaagcaaa
      121 tgcaacttgg caatcagcag atagcattca aactgcacca atgcctgcct cgatgaatca
      181 acctattgcc actcagccaa cctatcagca gccgacttac tctgcaccac aaccaatttc
      241 ggcagcacca gcaccaatgc ctgtttcaag tggtagtgat agctctcaag ttgaaacagt
      301 agggaactgc caagtggtgc gtgatgcgaa taatgctcct atctattcac aaattaccaa
      361 aggctgctac acagaaagtc actatacggt aggtaaaagt gatacgcttt atttaatcgg
      421 ttatctaacc ggcaccaatg ccaatcaaat tgcgcagcta aatggtttaa ataccggctc
      481 tccactgaaa gtcgggcaag tcttacgtgt tcgttaatag gcagagaaaa tgaacagaac
      541 ccttttagca tttgcaattt cttgtgcatt tttgaccgct tgtagctcgc ctactcagcc
      601 acaagttgaa gatccaaata tgttacctgc aggcacatta caaccgattg aaggctcagg
      661 cgcaagccaa gacagttaca gttggccgtc tgatattcaa tcaacgtcaa tgcctgacag
      721 tatgaaataa ttttaggaca atcatttaat gaaaaaacaa tttattcttc tccctttagt
      781 ctctgctgtt ttggcagcct gttcttcaaa caatccagca ccggtggtga atgcttccgg
      841 taataacgaa ttaagcccgg gcgtgatgca gcctgttgat gtgagtgcag gtgcaattaa
      901 ttcaagcggt ggctggcagt ctgatattca atctgcacca atgccaagct cgatgaatac
      961 cactccggtg caaacaatgc cccagccggt ttcagtgcca caacctgtgg cgactgtacc
     1021 gcagccaatt accaatccgg tgattaccga acaaccacca cagccgacaa ccacgaccaa
     1081 aatcgtgaaa aaaacgaaaa cggtcgagaa aaaagtggat caaaattttg agatcccgcg
     1141 tgatgccaac aatgccccaa tgtacaacca aattcaaaaa gggttctacg atggctctac
     1201 ctataccgta cgcaaaggcg acacaatgtt tttgattgcc tatattgtgg gtaaagatgt
     1261 aaaagaaatc gccgctctta ataatatgag cgaaccttac caattaagcg tagggcaaaa
     1321 actcaaaacc ggcaaatctg caacggaaac tattaccgtg gaagaaaaag tcaccgtacc
     1381 ggttgaacca caaattacct accaacaagg tgcaaacggc acaacctatg cttctggcgg
     1441 taatattact gggccggtaa aagcaggcac agccaacgct gatgtcacgc cggttaataa
     1501 cggtattaaa gcaacaactg caactgctcc ggcagtaact gcttctgtcg gtacagtaaa
     1561 tgcaacagag gaccgtaccg cctccaccat tcgtgctata gatggcacag caagtggtgc
     1621 cgtacaaagt agtaatactg tttctgcacc agcttcatca attaaatggc aatggccaac
     1681 cagcgggcgt gtaatttcag gcttctcctc agcagaagga ggtaataaag gcattgatat
     1741 tgccggcaat aaaggacaag atgtacgtgc tgccgccgca ggtaaagtgg tatatgccgg
     1801 taatgccttg cagggttacg gtaacttaat catcatcaaa cataccgatg atttcttaag
     1861 tgcttatgca cacaacaata ctattgaagt tgatgagcaa gaaacggtga aagccggtca
     1921 gaaaatcgcg actttaggta gtacaggaac caatacgaat aaattacatt ttgaaattcg
     1981 ttacaaagga aaatctgtcg atccggctcg ttatttacca agaaaataag agtaactttt
     2041 tagcataatc cgaccgcttg tgtactacaa gcggtcaatt tttaggaaaa ttatgcaaat
     2101 gaaaaaatat atcgcacaca tgataagttc gttactgctg atagcttgtt cggactaccg
     2161 agcaacacca actagcccaa gcgatgctta cacttgctct ccttcttttc gctactcttt
     2221 cttaaaaaaa acgtgcatac ctttattcag tatagccgac attcaattat tggatccaac
     2281 cgagccttca catcgcattt atattttgtt atctgaagat cgatttcaag ccgaagtctt
     2341 ctcaacgaag ctctctgaat cttctatgtt attggaggcg gttaaaggag gctatatttc
     2401 aaaagacgga aaagttcgtt tactcaaaaa agctgaaggt tggaaaattt actatgagtg
     2461 agactaaaat acgttgtgat tggtgtaaaa gcagtgaaat ttatatgaaa tatcacgacg
     2521 aagaatgggg aaaaccggag tttgacagcc tcaaactgtt tgagaaaatc tgccttgaag
     2581 ggcaacaagc cggactttct tggattacgg tgttgaaaaa acgggaagcc taccgccaag
     2641 cattccatca gtttaatcct gaaaaaattg ccaaaatggg tgaagaagaa attgatttgt
     2701 taatgcaaaa cactagctta atccaccacc gagcaaagtt agaagccatt ataaaaaatg
     2761 cgaaagctta tatcacaatg cagcaaaatg gcgaagactt cagtcggttt atttggtctt
     2821 ttgtgaatta tcagccgcaa attaatgatg taccaacact tgaagctgtt cctgctcata
     2881 cagaaacctc aaaagcaatg tcaaaagcgt tgaaaaaacg tgggtttgtg tttgtaggcg
     2941 agactacttg ctatgctttt atgcagtcaa tggggctggt taatgaccat ttgaataatt
     3001 gcgtgttcaa acataaataa acacaaaaaa ttaggcgacc atttggtcgc ctaacattaa
     3061 ttcagattat tgattgtttt gaacgtaatc aattgctgat tgaactgtag taattttttc
     3121 agcttcttca tctggaattt cgatatcgaa ttcttcttct aaagccatta ctaattcaac
     3181 agtgtctaaa gaatctgcac ctaaatcttc aatgaatgaa gcttctggtt ttacatcttc
     3241 tgctttcgcg ccaagttgat caacaatgat tttttttacg cgttcttcaa tgctcatttt
     3301 gtttttccta tagtgaatat cgcataagtg cgagagttaa atagtgtaat cgaaataagt
     3361 ttaaatacaa ccttttataa aaaggtcaaa ccagactatt cctatacacc agaatatatc
     3421 tgtttttcca ttctatcatt atgtttgtgt ttaatctata gagataaatc aaatttcatt
     3481 tgattaccct actttccgta attcatctcg catttgtgca atcactgctt gataatctgg
     3541 ctggtcaaaa atcgcagaac ctgccacaaa catatctgct cctgcggctg caatgtcggc
     3601 aatgttattt attttcacac caccatcaac ctctaagcga atgttataac cgctctcatc
     3661 aatacgacga cggacttctt tcaatttatc taaggtggac ggaataaatg attgtccgcc
     3721 aaagcccgga ttcacagaca tcaataaaac cacgtcaact ttatccatca cataatctaa
     3781 atagctcaat ggcgttgccg ggttaaacac caagcctgat ttacaaccta aatcacggat
     3841 aagctgtaaa tttcgatcta tatgttccgt tgcttcaggg tggaatgtaa tataatccgc
     3901 tccggctttg gcaaaatccg gaatcaaacg ctcaaccggt tttaccatta aatgcacatc
     3961 aatcgggcat tgaatgccgt aatcacgtaa ggctttgcag atcgccggcc cgaaggtcaa
     4021 attcggcacg tagtggttgt ccattacatc aaagtgaatt aagtctgcac cggcttttaa
     4081 cacctctgcc acatcatcac ccaagcgagc taaatcggca gataaaatag acggtgcaat
     4141 taaataagga ttcttactca tatttcctca ctatcaaaaa ataaatctac gttagattac
     4201 cataatttta acagcgaaaa gagtctctct tcctaaaaat ccgttttttt acaaaacac
//
LOCUS       DANLRK010000185         4223 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.185, whole genome shotgun sequence.
ACCESSION   DANLRK010000185 DANLRK010000000
VERSION     DANLRK010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4223
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.185"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            <1..486
                     /locus_tag="PXA33_002285"
     CDS             <1..486
                     /locus_tag="PXA33_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121583.1"
                     /translation="STFGSVNIESEIIKNGICLNFARQLISAKHPETKKRISLHLKIL
                     DFDKGRNTYLAPADLFILDEHLNEFILGNGGDEIEKISKNNKTQFSSTIRENQINFIR
                     ALLYMDYGIETPQEVKNAINSGELGRKIEKFRRENPNIENDKNFKFPTSVTLSNWYSK
                     A"
     gene            564..815
                     /locus_tag="PXA33_002286"
     CDS             564..815
                     /locus_tag="PXA33_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL5121584.1"
                     /translation="MQLQKTISTFTQPNHRGIYVMTYSEQSYLNVKDVCEMLQLNRST
                     IYRQIKQGIFPKPTKIGKSSRWKFADIKAFLEPKPQEAN"
     gene            815..1195
                     /locus_tag="PXA33_002287"
     CDS             815..1195
                     /locus_tag="PXA33_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL5121585.1"
                     /translation="MAGLSIICPFCGNTARIRGSSRPSLLSYQAQLTCSNCGQLKADF
                     VGQLINIKRAVFIECDEANQWDKPENELIKEGKIQGKDNAQRLKELRGSQQDLFAKVP
                     GQLPPEKITPAEKIAKRANLSKTH"
     gene            1506..2567
                     /locus_tag="PXA33_002288"
     CDS             1506..2567
                     /locus_tag="PXA33_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HDL5121586.1"
                     /translation="MKDLSPNQKQYTPFSAIMQKNLMDLLTKSARSPYSFSTVAKSTA
                     GRENPELHNTANSSTPLTRAIFIRSLHTPKERRNKACSSMVACSGQGSPLAVFGSPFV
                     AVFSPRYTLSPCTVRSVADSPLKLTNGLQKMNPSQITGKNRLNSFLFNNNQNDYSLLF
                     TNAKQSTPISAKISKNQDLCLTTLNRSPYASRAFAKSKAECGNSKEHQANNSTPLTRA
                     FFVRSSRTPKECQNKACSSMVACSGQGFALDCLPDVAVFHPVTRYRPTLWKMLAIAPK
                     NQHQELSKMKAFAYSFLCAIRTPETDTYQETVVRIISDSEQNARFQLTADYRLLLPRP
                     VAKIRLNSTAYTVKGGIYA"
     gene            2560..2724
                     /locus_tag="PXA33_002289"
     CDS             2560..2724
                     /locus_tag="PXA33_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121587.1"
                     /translation="MLNYSDIEQIRYFMDQAEEALDNNEEFKAVAELNTAQQFILALL
                     MNYEQGGENE"
     gene            2717..3349
                     /locus_tag="PXA33_002290"
     CDS             2717..3349
                     /locus_tag="PXA33_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121588.1"
                     /translation="MNNTTGQATQRNWQEIIANLQRLSESGESTIEIAKRYEAFSKTA
                     EQIQALVNNNGSEAEKEAFFAPVERFYLGLLETETRLKDAIAKDEENAQKLKEQKAAR
                     EAEQKRWDNLPFYAKDKFSDLICIESRETLEDKFFAISCMFDALAVPNLLEIGLREEG
                     KDSVIDRLAEMSKLYLLFVDEQFSLLANEIQSGKYGKLDLNGSQNKGDLC"
     gene            3343..3561
                     /locus_tag="PXA33_002291"
     CDS             3343..3561
                     /locus_tag="PXA33_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121589.1"
                     /translation="MLTHRITLPDGTEIEFFAETDKETFAEIQRDLEGLKLPTFAEFV
                     AQERKKHRAKQAVKKARKIAKLARRKNR"
     gene            3703..4188
                     /locus_tag="PXA33_002292"
     CDS             3703..4188
                     /locus_tag="PXA33_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121590.1"
                     /translation="METALQLAQHLTGWTEEENSQAILKNFLDWKETFGEKTREETKI
                     VETLLDWINENEASFIEYPFNQEQRTPPKVAGYRILANNSTKTNEYFFIYPKQFKETL
                     AEFPLLMACTKLHEAGILHKPPRPEKGYEFQYTIAPKITGGSKKRAYKIVPIALEEEN
                     E"
BASE COUNT         1533 a          869 c          804 g         1017 t
ORIGIN      
        1 agtacttttg gtagtgtaaa tattgaatct gaaatcatta aaaatggaat ttgtttaaat
       61 tttgcaagac aattaatatc agcaaagcac ccagaaacaa aaaaaagaat aagtttacat
      121 ctaaaaatac tggattttga caaaggcaga aatacttatt tagcacctgc agatttattt
      181 attcttgatg aacatctaaa tgagttcata ttaggtaacg gcggagatga aatagaaaaa
      241 atatcaaaga ataacaaaac tcaattcagc tcaacaatac gagaaaacca aatcaatttt
      301 attagagcct tgctttatat ggattatggg atagaaacgc ctcaagaagt aaaaaacgcc
      361 attaattcag gggaactagg cagaaaaata gagaaattta ggagagaaaa ccccaatata
      421 gagaacgata aaaatttcaa attccctaca agtgtaaccc ttagcaactg gtattcaaaa
      481 gcctaaaata ttggaaattt ccgaatctac tttggaaatt tccaaacttc taaaacctac
      541 tcacctcaca atctccccca acaatgcaac tacaaaagac aataagcaca ttcacgcaac
      601 caaaccacag ggggatttat gtaatgacct attcagaaca aagctattta aacgtgaaag
      661 atgtatgcga aatgctacaa ctcaaccgct caactattta tcgacaaatt aagcaaggga
      721 ttttccctaa gccaactaag atcggaaaat caagccggtg gaaatttgcc gacatcaaag
      781 catttttaga gccaaaacca caggaggcga actaatggca gggctttcta ttatttgccc
      841 gttttgtggc aataccgcac gaattagagg cagttctcgc cctagccttt taagttatca
      901 agcacagcta acttgttcaa attgcggaca attaaaagcc gactttgtag ggcagctaat
      961 caacatcaaa cgggccgtat ttattgagtg tgatgaagca aatcaatggg ataaacccga
     1021 aaatgaacta atcaaggagg gaaaaattca aggcaaagac aatgctcaac gcctcaaaga
     1081 actaagaggc tcacagcaag atttatttgc aaaggtgccg gggcaactac cgcccgagaa
     1141 gatcactccg gcggagaaaa tcgccaagcg ggcaaacctt tcaaaaacgc attaaacagg
     1201 gggataaatg gcaacaaaaa agccccgcaa ggcaaaccat acggagctat taaaatcgca
     1261 taatgcgaaa gatatgcaaa gaaattaaag caggcttgac gatttctgaa ggtgttagat
     1321 tctttctaaa gtctttaggg ataggtttat ttctagcatt gtttttttat tttttgccgt
     1381 atttagcaga atttataatc gccgtaaaaa aacaactagc aaaaaaatcg gcttactttc
     1441 aatgataaaa gcaagccgaa atgacaatcc aaatttttaa ctttaaataa agagtacaag
     1501 aagaaatgaa agatttatct ccaaatcaga aacaatatac gccattttcg gctataatgc
     1561 aaaaaaattt aatggattta ttgacaaaat ccgcccgttc gccttattct ttctctaccg
     1621 ttgcaaaatc aacggcaggg cgtgagaacc ctgaactaca taatacggcg aacagtagca
     1681 cgcctttaac ccgtgctatt tttattcgta gcttacacac acctaaagaa cgccgaaata
     1741 aggcgtgttc gtctatggta gcgtgtagcg ggcaaggttc gcccttggct gtttttggtt
     1801 cgccgtttgt agcagttttc tcaccccgtt acacgctatc gccctgcacc gtgagaagtg
     1861 tagccgatag tcctttaaaa ttaacaaacg gactacaaaa aatgaaccct agccaaatta
     1921 caggcaaaaa ccgcctaaac tcttttctat ttaacaataa tcaaaatgat tattcgctat
     1981 tatttactaa tgcaaaacaa tcaaccccga tttctgctaa aataagcaaa aatcaagatt
     2041 tatgcttgac aaccctcaat cgttcgcctt atgctagccg tgcttttgca aaatcaaaag
     2101 ccgagtgtgg aaactcgaaa gaacatcagg cgaacaatag cacgccttta actcgtgctt
     2161 tttttgttcg tagctcacgc acacccaaag aatgccaaaa taaggcgtgt tcgtctatgg
     2221 tagcgtgtag cgggcaaggt ttcgcccttg actgcttacc tgatgttgca gttttccacc
     2281 ccgttacacg ctatcgccct acattgtgga aaatgttagc gatagcccct aaaaatcaac
     2341 atcaggagct atcaaaaatg aaagcattcg cttattcatt cctttgtgct attcgcacac
     2401 ccgaaacaga tacttatcaa gaaaccgtag tacgcattat ttcagacagc gaacaaaacg
     2461 cccgttttca gcttacagcc gattatcgct tactactccc ccgccctgtt gcaaaaatcc
     2521 gcctaaattc taccgcttac acggtgaaag gggggattta tgcttaatta ttccgatatt
     2581 gaacaaatcc gctattttat ggatcaagcc gaagaggctt tagacaataa tgaagagttc
     2641 aaagccgtag cagaacttaa cacagcacaa caatttattc tggctttatt aatgaactac
     2701 gaacaagggg gtgaaaatga ataatacaac aggacaagct acgcaaagaa actggcaaga
     2761 aattatcgct aatttgcaga gactcagcga atccggggaa agcacaatcg aaatcgctaa
     2821 acgctatgag gcattttcta aaaccgccga gcaaatccaa gccctagtga ataacaacgg
     2881 cagcgaagcc gaaaaagagg cgttttttgc ccctgttgag cgtttttatc ttggcttact
     2941 agaaaccgaa acacgcttaa aagatgccat agccaaagac gaagaaaacg cccaaaaatt
     3001 gaaagagcaa aaagcagcta gagaggcaga gcaaaaaaga tgggacaacc tacctttcta
     3061 cgccaaagac aaattcagtg atttaatttg cattgaaagc cgagaaacgc ttgaagataa
     3121 attctttgcg atctcttgta tgttcgatgc cctagccgta cctaatctat tagaaatagg
     3181 cttgcgtgaa gaaggcaaag acagtgttat agaccgatta gccgaaatga gcaaactcta
     3241 tttactgttt gtagatgagc aattttcact actggcaaac gaaattcaaa gcggtaaata
     3301 tggcaagtta gacctaaacg gctcacagaa taagggggat ttatgctaac gcaccgcatt
     3361 acactccctg acggcacaga aattgaattt tttgcagaaa ccgacaaaga aacatttgca
     3421 gaaattcaaa gggacttaga gggcttgaaa ttgccaacct ttgccgagtt tgttgcccaa
     3481 gagcggaaaa aacaccgagc aaaacaagcg gtcaaaaaag cccgaaaaat tgcaaaatta
     3541 gcaagacgga aaaaccgcta gcacctatca aaaagactta ttattgcaga aaatcacgct
     3601 ttaataaaaa cgacctataa agcaatccgc aagaaatggt taagtttaac tgaaaatatg
     3661 agcggacagg ttcagcgggt agcgtgcgat agattcgcaa taatggaaac cgccttgcag
     3721 ttagcccaac atttaacagg ctggacagag gaagagaaca gccaagcgat tttaaaaaac
     3781 ttcctagact ggaaagaaac tttcggcgaa aaaacaagag aagaaactaa aatagtagaa
     3841 accttgctag attggataaa tgaaaatgag gcgagtttta tcgagtaccc attcaaccaa
     3901 gagcaaagaa caccgcctaa agttgccggc tatagaatac ttgcgaataa cagcactaaa
     3961 acaaatgagt actttttcat ttaccctaag caatttaaag aaacgctggc agaatttccg
     4021 ctattgatgg cttgcaccaa gttacacgag gcagggattt tacataagcc accacgccca
     4081 gaaaaaggct atgaatttca gtacacaata gcccctaaaa taacaggagg gagcaaaaaa
     4141 agagcttata aaatcgtacc gatagcattg gaagaagaaa acgagtaatt acagcaaaag
     4201 caagagtttc aattcttgct ttt
//
LOCUS       DANLRK010000186         4185 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.186, whole genome shotgun sequence.
ACCESSION   DANLRK010000186 DANLRK010000000
VERSION     DANLRK010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4185
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.186"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            6..1214
                     /locus_tag="PXA33_002293"
     CDS             6..1214
                     /locus_tag="PXA33_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HDL5121591.1"
                     /translation="MSKNLILILNCGSSSLKFAILDPKTGDEKLSGLAEAFHLEDARI
                     KWKLNGEKGNADLGAGAAHSEALTFIAEKLLSDELKAGIGAIGHRIVHGGEKFTSSVV
                     ITDEVIKGIEDATQFAPLHNPAHLIGIKEAFRIFPELKDKNVAVFDTAFHQTMPEHAF
                     LYALPYTFYKENGIRRYGFHGTSHFYITSQVAELAGKSVEQTNAIICHLGNGGSVSVV
                     RNGKCIDTSMGLTPLEGLVMGTRSGDLDPAIISFLYNNLGMSMDEIDTTLVKKSGLLG
                     LTGVTSDCRYAEDNYNDVSKPEAKRALDVYSYRLAKYIGSYMAILGDDHLDAIAFTGG
                     IGENSGLVRELTLNHLKLFGIKLDQERNLAARFGKDGVITADESTFKAVVIPTNEELV
                     IAQDTAKLAL"
     gene            1267..3402
                     /gene="pta"
                     /locus_tag="PXA33_002294"
     CDS             1267..3402
                     /gene="pta"
                     /locus_tag="PXA33_002294"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005542370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HDL5121592.1"
                     /translation="MSRTIILIPISTGVGLTSVSLGLVHALEQKGSKVGFLKPISQPI
                     SGEDTLDRSTTIIRAEQTTEVGEPFMLSEAETLIGQNQTDVLLEKVVERHQKLSKNNE
                     IVIVEGLIPTRKNSYANSINYEMAQALDAEIVLVAAPASDTPAQLKERVEAAASQFGG
                     RNNPNLLGVIINKFNAPVDDSGRTRPDLTEIFDSFQHSNNNVSEVEHLFAKSPIKLLG
                     CISWKAELIATRAIDVAKHLGASIINEGEIKARRIRGVTFCARNLPNMVDHFKAGSLL
                     VTSADRPEVLVAASLAVMNGIEIGAILLTGGYKIDSSIAKLCQQAFETGVPVFRIEGN
                     TWQTALSLQSFSLEVPVDDKERIAAIKEYMASKFNVGFIDEVSKAATRARRLSPAAFR
                     YQLTEYARQAKKRIVLPEGDEPRTVKAAALCAERGIAECVLLANPTDVQRVAEAQGVV
                     LGKGVTIIDPASIRENYVDRLVELRKAKGMTEVVAREQLLDTVVLGTMMLEANEVDGL
                     VSGAVHTTANTIRPPMQIIKTAPGSSIVSSIFFMLLPDQVLVYGDCAVNPDPTAEQLA
                     EIAIQSAESAKAFGIDPRVAMISYSTGTSGSGADVEKVKEATRIAQEKRPDLIIDGPL
                     QYDAAIMEDVARSKAPNSPVAGKATVFVFPDLNTGNTTYKAVQRSADLVSIGPMLQGM
                     RKPVNDLSRGALVDDIVYTIALTAIQATQ"
     gene            3516..>4185
                     /gene="can"
                     /locus_tag="PXA33_002295"
     CDS             3516..>4185
                     /gene="can"
                     /locus_tag="PXA33_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HDL5121593.1"
                     /translation="MEKIERLFANNHAWATKMKDEQSDYFKELAEHQKPTYLWIGCSD
                     SRVPAEKLTGLGPGELFVHRNVANMVIHTDLNCLSVVQYAVDVLEIEHIIICGHTNCG
                     GINAAIAQKELGLINNWLLHIRDIWFKHSHLLGKLPYEERSNVLTRLNVAEQVYNLGQ
                     SSIIKTAWEQGKSLSLHGWVYDVTDGFLIDQGVMATSRETLEITYRNAIAKITTEANE
                     KIVLN"
BASE COUNT         1303 a          782 c          861 g         1239 t
ORIGIN      
        1 tgaccatgtc taaaaactta atcttaatcc ttaactgcgg tagttcatct cttaaatttg
       61 ctattttaga tcctaaaaca ggtgatgaaa aattatctgg acttgctgaa gcttttcatt
      121 tagaagatgc acgtatcaag tggaagttaa acggggagaa aggtaatgct gatttaggtg
      181 caggtgccgc acatagtgaa gcacttacct ttattgctga aaaattgtta tctgacgaat
      241 taaaagcagg tatcggcgcg attggtcatc gtattgttca tggcggtgaa aaattcacct
      301 catcagttgt aattaccgat gaagtgatta aaggtattga agatgcaacg caatttgcac
      361 cattacacaa ccctgcgcat ttaattggta ttaaagaagc attccgcatt ttccctgaat
      421 taaaagacaa aaacgtagct gtatttgaca ctgcattcca tcagacaatg ccggaacacg
      481 cattcttata tgcacttcca tatacattct ataaagaaaa tggtattcgt cgctacggtt
      541 tccatggcac aagccacttc tatatcacct cacaagttgc agaattagca ggtaagtctg
      601 ttgagcaaac caatgccatt atttgccact taggtaacgg tggttctgtt tctgttgtac
      661 gcaatggtaa atgtattgat acctcaatgg gtttaactcc attagaaggt ttagtaatgg
      721 gtactcgctc tggtgactta gaccctgcga ttatctcttt cttatacaat aacttaggta
      781 tgtcaatgga cgaaattgat actactctag taaaaaaatc tggtctttta ggtttaactg
      841 gcgtaactag tgactgtcgt tatgcggaag ataattacaa tgacgtatca aaaccggaag
      901 caaaacgtgc tttagatgta tatagctatc gtttagctaa atatattggt tcatatatgg
      961 caattttagg cgatgaccac ttagatgcta tcgcatttac aggtggtatc ggtgaaaatt
     1021 caggcttagt acgtgaatta accttaaatc acttaaaatt atttggcatt aagttagatc
     1081 aagagcgtaa ccttgctgcc cgttttggta aagatggagt cattacagcg gacgagtcaa
     1141 catttaaagc tgttgttatc ccaactaatg aagaactcgt gattgcacaa gataccgcta
     1201 aattagcatt ataatcatag ggggcagata ttctgccctt tcactttctt ttaatttgga
     1261 aatattatgt ctagaacgat cattcttatc cctatttcaa ctggtgttgg tttaaccagt
     1321 gttagcttag gtttagtaca tgctttggag caaaaaggtt ctaaagtagg atttttaaaa
     1381 ccgatttctc aacctatcag cggtgaagat acgttagacc gctcaacaac gattatcaga
     1441 gcagaacaaa caacagaggt tggtgagccg tttatgttaa gcgaagctga aacattaatc
     1501 ggtcaaaacc aaacggatgt attgttagaa aaagtggtgg aacgccacca aaaattatca
     1561 aaaaataatg aaattgtcat tgttgaaggt ttaattccaa cgcgtaaaaa ctcatacgct
     1621 aacagcatta actatgaaat ggctcaagcc cttgatgctg aaattgtatt agttgcagca
     1681 ccggcctcag atacaccagc tcaattaaaa gagcgagttg aagctgccgc ttcacaattt
     1741 ggtggacgta acaacccaaa tttactgggc gttattatca ataaattcaa tgcaccagta
     1801 gatgattcag gtagaacccg tccagatttg actgaaattt ttgattcatt tcaacactca
     1861 aataataatg tttcagaagt cgagcatctt tttgctaaaa gtccaattaa gctgttaggc
     1921 tgtattagct ggaaagcaga acttattgct acacgagcaa ttgatgttgc aaaacattta
     1981 ggcgcttcaa ttattaatga aggcgaaata aaggctcgtc ggattcgcgg tgtgacattc
     2041 tgtgcacgta acttaccaaa tatggtcgat cattttaaag ccggtagttt attagttacc
     2101 tctgcggatc gtcctgaagt gttagttgct gcatcgttgg cggtaatgaa tggcatcgaa
     2161 attggtgcta ttttactaac cggtggttat aaaattgata gctcaattgc aaaactttgc
     2221 caacaagcat ttgaaaccgg tgtgcctgta ttccgtattg aaggtaacac ttggcaaacc
     2281 gcattaagcc tacaaagctt cagcttagaa gtgcctgtag atgataaaga gcgtattgct
     2341 gcaattaaag aatatatggc aagcaaattt aatgtaggtt tcattgatga ggtttctaaa
     2401 gccgctactc gtgcaagacg tttatcacct gcagcattcc gttaccaatt aaccgaatat
     2461 gcccgtcaag cgaaaaaacg cattgtttta cctgaaggag atgagcctcg tactgtgaaa
     2521 gcggctgcat tatgtgctga gcgtggcatt gcagaatgtg tgttattagc taacccaaca
     2581 gacgtacaac gtgttgctga agcacaaggc gtggtattag gtaaaggcgt aacgattatt
     2641 gaccctgcga gcattcgtga aaattatgtt gatcgcttag ttgaattgcg taaagcaaaa
     2701 ggaatgactg aagttgttgc tcgtgagcaa cttttagata cagttgtatt gggtacaatg
     2761 atgcttgaag ctaatgaagt ggacggttta gtctcaggcg cagttcacac aaccgctaac
     2821 actattcgcc caccaatgca gatcattaaa actgcaccgg gaagctctat tgtatcttct
     2881 atcttcttta tgctactacc tgatcaagta ttggtatatg gtgactgtgc ggtaaaccct
     2941 gacccaactg cagaacagct agcggaaatt gcgattcaat cagcagaatc tgccaaagcc
     3001 tttggtattg accctcgtgt tgcaatgatc tcctactcta ccggtacatc aggttcaggt
     3061 gcggatgtag aaaaagtgaa agaagcaact cgtattgccc aagaaaaacg tccagattta
     3121 atcattgatg gcccattaca atatgatgcg gcgattatgg aagatgttgc ccgctcaaaa
     3181 gcaccaaatt cacctgtagc aggtaaagca actgtatttg tcttcccgga tttaaatact
     3241 ggtaatacca cctataaagc ggtacaacgt tcggcagatt tagtctctat cggcccaatg
     3301 ttacaaggta tgcgtaaacc tgtaaatgac ttgtcccgtg gggctttagt tgacgacatc
     3361 gtttatacta ttgcgttaac tgcgattcaa gcaacgcaat aattccctcc caaatgaaac
     3421 caaatgccct taaaactaag ggcattttta ttttttacct acccgacttt tcaatttaat
     3481 catataatca agattagtgt taaaaaggag aatttatgga aaaaattgaa cgcttatttg
     3541 ccaataacca cgcctgggca actaagatga aagatgaaca atcggattat tttaaagaat
     3601 tagcggaaca tcaaaagcca acctatttat ggattggctg ttccgatagt cgtgtgcctg
     3661 ctgaaaaact cacagggtta ggccccggag aattatttgt tcatcgaaat gtagcaaata
     3721 tggtgattca cactgatctt aattgtctgt cggttgttca atatgcggta gatgtattag
     3781 aaattgagca tattattatc tgtggtcaca ctaattgtgg tggtatcaat gcggcaattg
     3841 cacaaaaaga acttggttta attaataact ggttgttaca tattcgtgat atctggttta
     3901 aacacagcca tttactaggc aaacttccct atgaagagcg atcaaatgtc ttgacccgat
     3961 taaatgttgc agaacaagtt tataatttag gtcagtcctc tattattaaa accgcttggg
     4021 aacagggtaa atccctctcg ctacacggtt gggtatacga tgttacagat ggtttcttga
     4081 ttgaccaagg tgttatggct actagccgag aaacattaga aattacctat cgtaatgcaa
     4141 tagctaaaat aacaaccgaa gcaaacgaaa aaatagtttt aaacg
//
LOCUS       DANLRK010000187         4172 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.187, whole genome shotgun sequence.
ACCESSION   DANLRK010000187 DANLRK010000000
VERSION     DANLRK010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4172
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.187"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(<1..1083)
                     /locus_tag="PXA33_002296"
     CDS             complement(<1..1083)
                     /locus_tag="PXA33_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL5121594.1"
                     /translation="MGIMSKSISIFNHKGGIAKTTTAFNVGWSLANQGYQVLLVDLDS
                     QCNLTGLVLGYDQCKEDSDLALFYNNRHHLTMEGIVEALINGQSPDNYLSGNVGQITQ
                     TLNPNLFLLPGHLNVSELDSQISVSLKIAAGVPATRNIPGNLPTLIKKVAEQNNIDYI
                     LYDLSPNVGGLNEVILMSSDYFIVPTSPDFFCWQAVGSLAKNIRKWHNELKAFKELNG
                     FNQSSYAIKNKPQFLGTIQQRYRPRKGQPAKSFEKWIERIRNEVITNFVPVLKTIDCA
                     IDDTAIYAALKSTNSDLEPFDLAHIADFNSLIAISQELRVPVFSLTKEQIKNSGQFGH
                     ALNTMDESKENFDQEFQSLAERIIQLT"
     gene            complement(1148..1579)
                     /locus_tag="PXA33_002297"
     CDS             complement(1148..1579)
                     /locus_tag="PXA33_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121595.1"
                     /translation="MKKMAIVALSAFFSMNAFANNNVVYSCTTTDNQTLKVTKEGGNY
                     VYSHGNTTFKNPVKEALKNPASEIAGGSQFTTISLELRNAGKSYIVGHIEADPKSPFE
                     ASVQIQDIKTGNDITSFECRSDKPIRHNFDRKLMRKSGFAA"
     gene            complement(1583..3685)
                     /locus_tag="PXA33_002298"
     CDS             complement(1583..3685)
                     /locus_tag="PXA33_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HDL5121596.1"
                     /translation="MKKAKSLKFLTACIMLTTMSGYANSNTVNVGNSISNFIENTTGY
                     RTGNLGVSGNNALTEYYPVLSDYMHYSIGGGSAISAPASRGRPNQYGIGIGWKSNLTC
                     GNFDIKLSIKNQLNGITEGFKDLYGNIIESATGAVASLPAMIIQRANPQLYDILSNGL
                     YQGKLDFNNFKTSCEAMSNQLADLAMDSKLAKMAGLHRAAEIAETEPDAMAAKKKMDK
                     KDAEKGIPWVGGEQRGGKNQKPMAIVADIVGAGYNLALGRGPLEKGDIPIASCDGMLC
                     TEWKNPSEAAAYASEVIGETFLDACETCEQSSSGAKAGKGIAHFIEQTTIEKATKLEA
                     ALNHLDSLTPEQLNSLSTSTVSVTKGVLEAIAASPDAALLGARLSQELAISKEMEKLL
                     LIRRAMLAGMREPNVIKSSLVEEAERNLKLLDREIEQVKLEMELQRSINGNTALAAIT
                     QQMNEQALAIDDNAMGDGNRRFANLSNQAGANNLNTSGIANSAQNAVGDRYIAIPKSD
                     GSGMSDFYGAYKASPTNIVSNVNVGSYTQIAQMNGNANEQATALIKQFEGYLSHGKWD
                     VNANRAGYGSDTYTTADGMVHRVQKGTYVSREDAERDLARRMPQFMNVARGNVGADVW
                     AKLTPQAQAALTSVSYNYGSLSKLESVQKAAAVSAQSGDMTALANSIRNLQGHNNGVN
                     ARRRNQEADYIINAPKGN"
BASE COUNT         1158 a          824 c          798 g         1392 t
ORIGIN      
        1 agtcagttgt ataattcgtt ctgctaatga ttgaaattct tggtcaaaat tttctttact
       61 ttcatccatt gtattcaaag catgtccaaa ttgacctgag tttttaattt gctcttttgt
      121 taaggaaaat acgggaactc gtagctcttg gctaatagca atcaacgaat taaagtcggc
      181 tatgtgagcc aaatcaaatg gctctaagtc agaattcgtt gatttaagag cagcataaat
      241 agctgtatca tcaatagcac aatcaatagt ttttaatacc ggcacaaaat tcgtaatgac
      301 ttcatttcta attcgctcaa tccatttttc aaatgatttg gccggttgcc cttttcttgg
      361 tcgataacgt tgctgaatag tacctaaaaa ttgtggctta tttttaattg cataagatga
      421 ttggttaaaa ccattaagtt ctttaaatgc ttttaactca ttatgccatt tgcgaatatt
      481 tttagcgagc gaaccaactg cttgccagca gaaaaaatca ggtgatgtag gtacaataaa
      541 gtaatcacta gacattagga taacttcatt taatccaccc acatttggac taagatcata
      601 gagtatgtaa tcaatattgt tttgttcagc tactttctta attaaagtcg gaagattgcc
      661 gggtatattt cttgttgctg gaacaccggc agcaattttt aatgatacac taatttgcga
      721 atcaagctcg gaaacattaa ggtgaccagg taacaagaac agatttggat tcagggtttg
      781 agtgatttgg ccaacatttc cactaaggta gttatcaggg ctttgtccgt ttatcagtgc
      841 ttcaacaata ccttccatag tcaaatgatg tctattattg taaaaaagcg ctaaatcact
      901 atcttctttg cattgatcgt agccaagtac cagaccggtt aggttacatt gtgaatccaa
      961 atcgactaac aatacttgat agccttgatt tgctaaactc caaccaacat taaatgcagt
     1021 tgtagtttta gcaatacctc ctttatgatt aaaaatactg atagacttac tcataatacc
     1081 cataattcta tgaaataatt aacccaccga actcatttga gaatggtggg ttaatgcttt
     1141 gctttagcta ggctgcaaat ccagattttc tcatcagttt gcgatcaaag ttgtggcgaa
     1201 taggcttatc ggaacgacat tcaaatgatg taatatcatt tcctgtttta atatcctgaa
     1261 tctgaacact tgcttcaaaa ggtgatttag ggtctgcttc tatatgacct acaatataag
     1321 attttcctgc atttcttaat tcgagcgaga ttgttgtgaa ttgtgaacca cctgcaatct
     1381 ccgatgccgg atttttgaga gcttctttta caggattttt gaatgtggta ttaccgtgag
     1441 agtacacata gtttcccccc tctttggtaa cttttaacgt ttggttatct gtagttgtgc
     1501 aagaatacac tacattgtta tttgcaaaag cattcattga gaaaaatgct gataaagcta
     1561 caattgccat ttttttcata ggttagtttc cttttggtgc gttgataatg taatcggctt
     1621 cctggttacg tcttcgagca ttaacaccat tattatgccc ttgtaaattt cggattgagt
     1681 tagctaaagc cgtcatatca cctgattgtg cggagacggc agccgctttt tgaacacttt
     1741 ctagtttaga taatgaccca tagttgtatg ataccgacgt taatgctgct tgtgcttgag
     1801 gagtcaattt agcccaaacg tcggcaccaa cattacccct tgcaacattc ataaattgag
     1861 gcattcttcg tgctaaatca cgttcagcat cttcccggct tacataagtg cctttttgaa
     1921 cccgatgaac cataccatct gcagtagtat aggtgtcaga gccatatccg gcacggttag
     1981 catttacatc ccatttcccg tggcttaaat atccttcaaa ctgtttgata agagccgttg
     2041 cttgttcatt cgcattgccg ttcatttggg caatttgggt atagctccct acattcacgt
     2101 tactgacaat gtttgttggg gaggctttat atgctccata aaaatcactc atgcctgagc
     2161 cgtctgattt ggggatagca atatatctat cgccaacggc attttgtgca gaattagcaa
     2221 tgccactggt gttgagatta tttgctccgg cttgattgct taaattggca aagcgacggt
     2281 ttccatcacc catcgcatta tcatcaatag caagagcttg ttcgttcatc tgttgagtaa
     2341 ttgcagctaa tgctgtatta ccgttgattg aacgttgcag ttccatttcg agtttaactt
     2401 gctcaatttc tctgtcaagt aatttgaggt ttctttctgc ttcttctact aaggaagatt
     2461 ttataacatt tggctcacgc ataccggcta acattgctcg gcgaatcagt agcaattttt
     2521 ccatttcttt agaaatagct aattcttgcg ataaccttgc acccagcaag gctgcatccg
     2581 gcgaagcagc aatggcttct aatacgcctt ttgttacact gaccgttgat gtactcagtg
     2641 aattgagttg ttccggcgtt aaactatcta agtgattcag tgccgcttct agctttgtgg
     2701 ctttttcaat agttgtttgt tcaataaaat gggcgattcc tttcccggct ttagctccac
     2761 ttgaactttg ttcacaagtt tcacaggcat caaggaatgt ttcaccaatc acttcacttg
     2821 cataggcagc agcctcgctt ggatttttcc attccgtaca aagcatacca tcacagcttg
     2881 caataggtat atctcctttt tctaatggtc ctcgtcctaa agcgaggtta tatccggctc
     2941 caacaatgtc agcaacgata gccattggtt tttgattttt accaccacgt tgttcgccac
     3001 ctacccaagg gatgcctttt tcagcatctt ttttatccat tttcttcttg gctgccattg
     3061 catcaggctc tgtttcagca atttcagcgg ctcggtgtaa tccggccatc tttgctaatt
     3121 tactgtccat tgctaaatca gcaagttggt tagacatggc ttcgcaactg gttttgaagt
     3181 tgttgaagtc aagttttcct tgatatagac cattagaaag aatgtcatac agttgaggat
     3241 ttgctcgttg gataatcatc gccgggaggc tggcaactgc accggttgct gactcaataa
     3301 tattgccata aagatcttta aaaccttcag taataccatt aagctggttt ttaatggaaa
     3361 gtttaatatc aaagttgcca caagttaaat tcgatttcca gccaatgcca atcccatatt
     3421 gatttggtct gccacgggaa gctggtgcgg aaatggcact acctcctcca atggagtagt
     3481 gcatataatc agaaaggaca gggtagtatt ccgtgagagc gttattacca gaaacgccta
     3541 gattaccagt cctatagcct gttgtatttt caataaaatt actaatgcta ttgccaacat
     3601 taacggtatt actatttgcg tatccgctca tggttgtgag cattatacaa gcggttagaa
     3661 atttgagact ttttgctttt ttcatttatt tatcctaata gtgctgataa tttaatgagt
     3721 atgtgtactt ttccagtcca catgacctaa aaaaacttgc ccttgttttt tacagcaaga
     3781 atagggcctc cataatgccc agctgtaatt ctcagtattg gagaaactat ctgaatgggt
     3841 agcccaatca ccactgtgcg gaaagacttt agggtaggat tcggaagtaa ataccttaac
     3901 tgttacatct gagggtttat tagcaggact agcacttgcc atagctgaaa gcagtagtag
     3961 tggtaaatag taaaatgtct tagcttgcat aaatgctcca caaatagagt aattcacgtt
     4021 taccttaaaa gatgatttgg ttagaagaaa tcggaaattg ttttttggtt ttggagagtt
     4081 tttgttagta tataagttaa tgtaacaagg gagagtggat gtatgagcaa tagaatacct
     4141 aaagcctgtt atgcttaccc aaaatcgccg aa
//
LOCUS       DANLRK010000188         4169 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.188, whole genome shotgun sequence.
ACCESSION   DANLRK010000188 DANLRK010000000
VERSION     DANLRK010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4169
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.188"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            143..2077
                     /gene="iolD"
                     /locus_tag="PXA33_002299"
     CDS             143..2077
                     /gene="iolD"
                     /locus_tag="PXA33_002299"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HDL5121597.1"
                     /translation="MQTTRLTVAQALVQFLDNQYLDVDGKEIKFVHGVAAIFGHGNVL
                     GIGQALAQNAGGLTLYQGRNEQGMAHLAMGFAKQNLRQRIIACTSSVGPGAANMVTAA
                     ATATANRIPLLLLPGDVFATRQPDPVLQQIEQPYDLSISTNDAFRAVSKYWDRVQRPE
                     QLMSACINAFRVLTDPAETGAVTIALPQDVQAESFDFPNSFLQKRVHRLERTPPTTAM
                     IKQAVTLIQTKKKPLIVCGGGVRYSQAGQILQKFAKQFGIPFAETQAGKSAVVSDHAL
                     NVGGVGETGCLAANRLAKEADLVIGIGTRYTDFTTSSKWIFQNPNVQFLNINVARFDA
                     YKLDGVQLVADAKTALEALSQAIPTDYQAQWENEISQAKADFADEIQRLRAVQYHPDF
                     TPEINDNLDHKAVHNEFIQLTGSQLTQTQVLGIVNETIDNNAIIVAAAGSLPGDLQRT
                     WLSKGEETYHLEYGYSCMGYEVSAAVGVKIARPEQEVYALLGDGSYLMAHTELVTSIQ
                     EGKKINVVLFDNMANGCINNLQIGNGMDSFGTEFRFRNGETNQLNGGLVPIDFAKNAE
                     AYGCKTYRVTNEAELRAALDDAKKQTVSTLIDIKVLPKTMAHGYGCWWHVGVAEVSES
                     ERIQQARQRSEEQLAKARKY"
     gene            2204..3100
                     /gene="iolE"
                     /locus_tag="PXA33_002300"
     CDS             2204..3100
                     /gene="iolE"
                     /locus_tag="PXA33_002300"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011817366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HDL5121598.1"
                     /translation="MKAENIQLGIAPIGWTNDDLPELGAENTFEQCVSEMALAGYTGC
                     EVGNKYPRDVAVLKHKLDVRGIQICNAWFSTFFVDGKREETIAEFIKHRDFLHAMGAK
                     VIGCSEQSRSIQGTTKSVFKEKPIFNDEDWKRMAEGYNELAKLAAEKGMKVCLHHHMG
                     TGIQTPEEVDRYMAEVNDDVYLLFDSGHLYYSEGCQKAMLGVLEKYIDRIIHVHLKDV
                     RDEVMAEVKAKDLSFLEGVKKGTFTVPGDGVIDFRPIFDILEKHDYKGWMVVEAEQDP
                     ALANPFEYALKGRKYIRETAGV"
     gene            3102..4112
                     /gene="iolG"
                     /locus_tag="PXA33_002301"
     CDS             3102..4112
                     /gene="iolG"
                     /locus_tag="PXA33_002301"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005537901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HDL5121599.1"
                     /translation="MLKVGIIGAGRIGRVHSESISKYVKGAEIKAISDVKITEELTAW
                     AKSMGIPEVYDDYKKILQDPEIDAVLVCSSTNTHAPISIEAAQAGKHVFCEKPVDADP
                     AKIREVLSAVEKAGVKFQVGFNRRFDHNFKAIKDRVAAGDIGEPHVIRVTSRDPEAPP
                     IEYVKVSGGMFFDMTIHDFDMIRYLSGSEVEEVFAVGTVLVNPEIGKAGDIDTAVITL
                     KLKNGAIGVIDNSRKAAYGYDQRAEVFGSKGAIHITNDTGSTAVFSGENGVVAEKPKY
                     FFLERYMQSFADEISCFVDSVVNDKPTLVNGNDGLQPVLIALAAKKSLEENRPVKLSE
                     VA"
BASE COUNT         1218 a          910 c         1022 g         1019 t
ORIGIN      
        1 ggcaataact tcgcagaaat gagaactgga tcacatcttt ttttcgattt ccttgctatt
       61 ataaatgaaa taaatttttc atttgtaaaa atcagtagaa aaccgaccgc ttgcaaaagc
      121 gatgtaagcc agaggaggct ttatgcagac tactcgttta acggttgcac aggcgttagt
      181 gcaatttttg gataatcaat atttagacgt ggacggcaaa gagattaaat tcgttcacgg
      241 tgttgccgct attttcggcc acggcaatgt gctgggaata ggtcaggcgc ttgctcaaaa
      301 tgccggtggt ttaacgctct accaaggcag aaatgagcaa ggtatggcac atttggcgat
      361 gggctttgct aagcaaaatc tgcgtcaaag aattattgcc tgtacttcct cggtcgggcc
      421 gggggcggca aatatggtta cggctgcggc aacggcaaca gcgaaccgta ttcctcttct
      481 tttattaccg ggcgatgtgt ttgcgacccg ccagccggat ccggtactgc aacaaatcga
      541 acaaccttac gatttaagca ttagcactaa cgatgcgttc cgtgccgtca gtaaatattg
      601 ggatagagtg caacgccctg agcagttaat gagtgcttgt atcaatgcgt ttcgtgtgct
      661 gaccgaccct gctgaaaccg gtgctgtcac cattgctctg ccgcaagatg tacaagcgga
      721 gagttttgat ttcccgaaca gcttcctgca aaaacgagtg catcgcttag agcgtacacc
      781 gccgacaaca gcgatgatta agcaagcggt aacattaatt caaaccaaga aaaaaccgct
      841 gattgtctgc ggtggcggtg ttcgctactc gcaagcgggt caaattctgc aaaaatttgc
      901 taaacagttc ggcattccat ttgctgaaac ccaagcgggt aaaagtgcgg tggtgtccga
      961 tcacgcattg aatgtaggcg gagtgggaga aaccggttgt ttagcggcaa accgtctggc
     1021 aaaagaagcg gatttagtga ttggcattgg tacacgctac accgacttca cgacttcttc
     1081 caaatggatt ttccaaaacc caaacgtgca gtttttgaac attaatgtgg cacgttttga
     1141 tgcgtacaaa ttagacggcg tgcaactggt tgccgatgca aaaactgcat tagaggcgtt
     1201 aagccaagcc attcctactg attatcaagc tcaatgggag aatgaaattt cacaggcgaa
     1261 agcggatttt gctgatgaaa ttcaacgcct gcgcgctgtg caatatcacc ctgatttcac
     1321 ccctgaaatt aacgataatc ttgatcataa agcggttcac aatgaattta ttcagctcac
     1381 cggttcccag ctcacccaaa cccaagtgct gggtattgtg aatgaaacca ttgataacaa
     1441 tgcgattatt gttgccgccg ccggaagttt gccgggtgat ttacaacgca cttggttaag
     1501 taaaggtgaa gagacttacc accttgaata tggctactcc tgtatgggct atgaagtgag
     1561 tgcggcagtc ggcgtgaaaa ttgcccgccc cgaacaagag gtctatgcct tgctggggga
     1621 cggctcttac ttaatggcac acaccgaatt agtgacctca attcaagagg gcaaaaaaat
     1681 caacgtggtg ctgttcgaca atatggcaaa cggctgtatc aacaacctgc aaatcggcaa
     1741 cggtatggac agtttcggta cggaattccg cttccgcaat ggcgaaacta accaactcaa
     1801 cggcggttta gtgccgattg attttgccaa aaacgccgaa gcatacggct gcaaaaccta
     1861 tcgggtaact aacgaagccg aattgcgtgc cgcccttgat gatgcgaaaa aacagaccgt
     1921 ttctacctta atcgatatta aagtgttgcc gaaaacaatg gctcacggct atggctgctg
     1981 gtggcacgtt ggtgtcgcgg aagtgtcaga gagcgagcgt attcaacaag cccgccaacg
     2041 ctccgaagag cagttagcta aggctcggaa gtattagtta aaaaaacaac tccccacttt
     2101 gagctacgag gggagaatat caagcggtca aatttgcaaa atttttgata aaaataaccg
     2161 cttgcatcca aatttatctc tataaatcaa acgaggtttc tctatgaaag ctgaaaacat
     2221 tcaattaggc attgccccaa tcggttggac gaacgatgac ctgccggaac tgggagcgga
     2281 aaacactttc gagcagtgtg tgagtgagat ggcacttgcc ggctacacgg gctgtgaagt
     2341 gggtaataaa tatccgcgtg atgttgccgt gttaaaacac aaactggacg tgcgtggcat
     2401 tcaaatctgt aatgcgtggt tcagcacctt ctttgtggac ggtaaacggg aagagaccat
     2461 tgccgagttc atcaaacatc gtgatttctt gcatgcaatg ggggcaaaag tgatcggctg
     2521 ttccgaacag agccgtagta ttcaaggcac aaccaaatct gtgttcaaag aaaaaccgat
     2581 tttcaacgat gaagattgga aacgtatggc agaaggctat aacgaattgg cgaaacttgc
     2641 cgccgaaaaa ggaatgaaag tctgcctgca tcaccatatg ggaaccggta ttcaaacacc
     2701 cgaagaggtc gatcgctata tggcagaagt gaatgacgat gtctatctgt tgttcgattc
     2761 agggcatttg tactattccg aaggctgcca aaaagcgatg ctcggcgtac tggaaaaata
     2821 tatcgaccgt attatccacg ttcacttaaa agatgtgcgt gatgaagtca tggcggaagt
     2881 aaaagcaaaa gatttaagct ttttagaagg cgtgaaaaaa ggcaccttta ctgtaccggg
     2941 cgatggtgtc attgatttcc gtccgatttt cgatatttta gaaaaacacg actacaaagg
     3001 ctggatggtt gtagaagccg aacaagatcc ggcacttgcc aacccgtttg aatacgccct
     3061 caaaggcaga aaatatatcc gtgaaaccgc aggagtttaa tatgttaaaa gtaggtatta
     3121 ttggtgcagg ccgtattgga cgtgtgcatt ctgaaagtat tagcaaatat gtcaaaggtg
     3181 ctgaaatcaa agcaatctct gatgtaaaaa ttaccgaaga attgaccgct tgggcaaaat
     3241 cgatgggtat tccggaagtt tatgatgact ataaaaaaat cctgcaagat ccggaaattg
     3301 atgctgtatt ggtctgctct tccaccaaca ctcacgctcc gatttcgatt gaggcagcac
     3361 aggctggcaa acacgttttc tgtgaaaaac cggtagatgc agacccagct aaaatccgtg
     3421 aagtattgag tgcagtcgaa aaagccggtg taaaattcca agtgggcttt aaccgccgct
     3481 tcgaccacaa cttcaaggcg attaaagacc gtgtggcggc aggcgacatc ggcgaaccgc
     3541 acgttatccg agtaacctct cgtgatccgg aagccccgcc aatcgaatat gtgaaagtct
     3601 ccggcggaat gttctttgat atgaccattc acgacttcga tatgattcgt tatttatccg
     3661 gcagcgaagt ggaagaagtg tttgcagtcg gcaccgtatt agtaaaccct gaaatcggca
     3721 aagcgggcga tattgatact gccgttatca cgttgaaact gaaaaacgga gcaatcggcg
     3781 taatcgacaa cagtcgcaaa gctgcctacg gctacgacca acgtgcggaa gtgttcggct
     3841 cgaaaggggc aatccatatc actaatgata ccggctcaac tgccgtattc tcaggtgaaa
     3901 acggcgtggt ggcggaaaaa ccaaaatact tcttcttaga acgttatatg caatctttcg
     3961 ccgatgaaat cagctgtttc gtagattctg tggtaaacga caaaccaacg ttagtaaacg
     4021 gcaatgacgg cttacaaccg gtacttattg ccttagctgc gaaaaaatca ctggaagaaa
     4081 accgaccagt gaaattgagt gaagtggcgt aattgatcat aaatacaagc ggtcggtttt
     4141 actcgctaca ctcgcacttc gtgccatcg
//
LOCUS       DANLRK010000189         4141 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.189, whole genome shotgun sequence.
ACCESSION   DANLRK010000189 DANLRK010000000
VERSION     DANLRK010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4141
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.189"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            84..725
                     /gene="sodA"
                     /locus_tag="PXA33_002302"
     CDS             84..725
                     /gene="sodA"
                     /locus_tag="PXA33_002302"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005708524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HDL5121600.1"
                     /translation="MAYTLPELGYAYDALEPHFDAKTMEIHHSKHHQAYINNANAALE
                     AHPELLEKCPGALIKDLSQVPAEKRIAVRNNVGGHVNHTLFWKGLKTGTTLQGALKEA
                     IERDFGSVEAFQSEFEKAATTRVGSGWAWLVLEEGKLAVVSTANQDNPLMGKEVAGVS
                     GYPILVLDVWEHAYYLNYQNRRPDFIKAFWNVVNWDEAARRFEEKVATCGCAK"
     gene            complement(788..1912)
                     /gene="alr"
                     /locus_tag="PXA33_002303"
     CDS             complement(788..1912)
                     /gene="alr"
                     /locus_tag="PXA33_002303"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDL5121601.1"
                     /translation="MKPATATIDSQALRHNIRLIKSFAPQQKLLAMIKANAYGQGLLP
                     AAGTLADMVDGFGVARLREALEIQETGYTGKIVLVEGFFDREELLKTLSRKFDTVIHN
                     LEQLELLEQVAKEWQAEQQKGFWTRKAKIYFPINIWLKIDTGMHRLGIHPEQVDHFVE
                     RLKNCALVNSISFVSHFSRADELDCGYTEKQIAVFEQATQKYQDHDRSISASSGILYW
                     KQAHYDWVRPGIIMHGISPHYTPITDLGFKPVMTLSSSLIAVRTHKAGEPVGYGGAWV
                     SPKDTKLGVTAMGYGDGYPRNAPEGTPVLINGRIVPIVGRVSMDMLTVDLGAESQDKV
                     GDKAIFWGEQLLIEDVAKHIGVISYELITKLTPRVIFEYK"
     gene            2042..4003
                     /gene="recD"
                     /locus_tag="PXA33_002304"
     CDS             2042..4003
                     /gene="recD"
                     /locus_tag="PXA33_002304"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HDL5121602.1"
                     /translation="MLNLLLELKQEKAISDLNYQFARFIDNKQKPYGYSPLEQDLAVL
                     AAALVSYQVGLGHTALRLENLSLSSLFELNSKPQTYHLLEQILQKIEQIPPLEWQSVL
                     KNHIAFSYSPETIKPMLFQNSLLYFYRYWQAEHRIAGYLQQAVDFSEKFANTELNQSI
                     LNQLFTGKAQTIDWQKIAVATALEKQFSLISGGPGTGKTTTVVKLLLGLQLKQQALQK
                     PYLQIALAAPTGKAAARMKESLESKLAEEKGLSEELKRSIPTEAVTIHRLLGARPLTE
                     KTKYHTKNPWHYDLVVLDEASMIDLSMMEKVVQALKPNSRLIMLGDKDQLASVEAGSI
                     MGELGSFIDNGYSQSHCDYLKAVTDYQIEAGNALPIGDCLSHLKHSYRFGEKAWIGEL
                     ANAVNDQKINLSWEIFDKYKNSDKLENFCYPETKETTDKSSWIEKSVQMVVDKAVELY
                     RDYLQAVQKREKNPDSVSVKAIFEKFQKVRILSALRVSELGVERLNMAIAEGLRKASL
                     VKFNHSRDRYIGKPILMTENMPINSIASGDVGIILPDENGESRVYFDSLQENREHHSL
                     PLGRVSNYEAAYIMTVHKSQGSEFEHTVLVLPHSISPVLTKELIYTAITRAKDKFTLF
                     GSEKVWKYSVGAKTERQSGLKEQIRGKFS"
BASE COUNT         1287 a          801 c          889 g         1164 t
ORIGIN      
        1 gtgcattttt tgtgtgtttc ttgataaaat gtactcggag tttaactctg attaacctat
       61 ttactatttg agaggaatta actatggcat atacattacc agaattaggc tacgcttatg
      121 atgcgttaga gccacatttc gatgctaaaa caatggaaat ccaccattct aaacatcacc
      181 aagcttacat caacaatgca aatgcagcat tagaagctca ccctgaatta ttagaaaaat
      241 gtccgggtgc attaatcaaa gatttgagcc aagtgcctgc agaaaaacgt attgcagtgc
      301 gtaacaacgt aggtggtcat gtaaaccaca ctttattctg gaaaggttta aaaacaggca
      361 ctaccttaca aggtgcatta aaagaagcga ttgagcgtga tttcggttca gttgaagctt
      421 tccaatcaga atttgaaaaa gcagcgacta ctcgtgttgg ttcaggctgg gcgtggttag
      481 tattagaaga gggtaaatta gccgttgttt caaccgctaa ccaggacaac ccactaatgg
      541 gtaaagaagt ggcaggcgtt tcaggctatc cgattttagt cttagacgtt tgggaacacg
      601 cttactactt aaactaccaa aaccgccgtc cggatttcat taaagcattc tggaacgttg
      661 taaactggga cgaagccgct cgccgttttg aagaaaaagt agcaacctgc ggttgtgcga
      721 aataatattt tcgatcttga ataagataca agcggtaaaa tttgttgaaa attttaccgc
      781 ttgttgtcta tttatattca aaaatcacac gaggagtgag tttagtaatc agctcatagc
      841 taatcacgcc aatgtgtttc gccacatctt caattaatag ttgctctccc caaaaaatgg
      901 ctttatcgcc cactttatct tggctctctg cacctaaatc gaccgtcagc atatccatcg
      961 aaactcgacc gacaatcggc actattctac cgttaatcaa cacaggggta ccttcaggag
     1021 cattgcgagg gtagccatcg ccgtagccca ttgcagtcac gccgagtttg gtatctttag
     1081 ggcttaccca agcaccacca tagcctacag gctcgccggc tttatgggta cgcacagcga
     1141 tcagggaaga ggagagcgtc attaccggct taaaacctaa atcggtaatc ggcgtatagt
     1201 ggggagaaat gccgtgcata ataatacccg ggcgaaccca atcataatga gcctgtttcc
     1261 aataaagaat accgcttgag gcggaaatac tgcggtcgtg atcctgatat ttttgcgtag
     1321 cttgctcaaa caccgcaatt tgtttttcgg tatagccaca atcgagttca tcggcacggc
     1381 taaaatgact cacaaagctg attgaattga ccaaggcaca atttttcaaa cgttcaacaa
     1441 agtgatcgac ttgctccggg tgaatgccta agcggtgcat tccggtgtcg atttttagcc
     1501 aaatgttaat cgggaagtag attttcgctt tacgagtcca aaagcctttt tgttgctctg
     1561 cctgccattc ttttgctact tgctcgagta attccagttg ttcgaggtta tgaatgacgg
     1621 tatcaaactt gcgggaaagg gttttgagta gctcttctcg gtcaaaaaaa ccttcaacta
     1681 acacaatttt gcctgtgtat ccggtttctt gaatttctaa ggcttcacgc aaacgggcaa
     1741 ctccaaagcc atcaaccata tctgctaatg tgccggcagc cggcaataag ccttgcccgt
     1801 aagcattggc tttgatcatc gccaataatt tttgctgtgg agcgaaagat ttgatcaggc
     1861 gaatattatg gcggagggct tgactatcaa tggttgctgt tgctggtttc atacttatct
     1921 acatagttaa aatcacaaga aaggctaaat tatacgccta aaataaaaaa tcccttagaa
     1981 aataatgcaa aaaagattag tattatcact taatgttagg tggtcgattt aaggtttgat
     2041 tatgctgaat ttattattgg aacttaaaca agaaaaggcg atttctgact taaattatca
     2101 atttgcccgt tttattgata ataagcagaa accctatggg tattcgcctt tggagcaaga
     2161 tttagctgtg ttagccgctg cgttggtttc ttaccaagtg ggacttgggc atacggcatt
     2221 acgtttggaa aatctcagtc tttcttctct ttttgaatta aatagcaagc cacaaactta
     2281 tcatttgctc gaacaaattt tgcaaaaaat tgagcaaatt ccaccgcttg aatggcagag
     2341 tgtgctgaaa aatcatatcg cttttagcta ttctcccgaa acaattaaac cgatgttatt
     2401 ccaaaacagc ttgctctatt tttatcgcta ctggcaagcg gagcatcgca ttgccggtta
     2461 tttacaacaa gcggttgatt tttcggaaaa atttgcaaat accgaattaa accaaagcat
     2521 tctcaaccaa ctattcactg ggaaagctca aactatcgac tggcaaaaga ttgccgttgc
     2581 aacagcatta gaaaagcagt ttagcttaat ttcaggtggg ccgggaaccg gaaaaacaac
     2641 tacggtagta aaattattac tcgggctgca attaaaacag caagcactac agaaaccata
     2701 tttgcaaatc gctctggctg caccaaccgg aaaagcagca gcaagaatga aagagtcgct
     2761 ggaaagcaag ttggcggaag aaaaggggtt atctgaggag ttaaaacgct ctattccaac
     2821 tgaagcagtg actattcatc gcttgcttgg tgcgagacca ttaactgaga aaaccaaata
     2881 tcataccaaa aatccgtggc actatgattt ggtcgtatta gatgaggctt caatgattga
     2941 tttatcaatg atggagaaag ttgttcaagc gctcaaacca aactctcgct tgataatgct
     3001 gggagataaa gatcagcttg cttctgtgga agctggttca attatggggg aacttggctc
     3061 ttttattgat aatggttata gccaatccca ttgcgattac ttaaaagccg ttaccgacta
     3121 ccaaattgaa gccggtaatg cgttgccgat tggtgattgt ttatctcact taaaacatag
     3181 ctatcgattt ggcgaaaaag cctggattgg agagcttgca aatgcagtaa atgatcaaaa
     3241 aattaacctt tcgtgggaga ttttcgacaa atataaaaac agtgataagt tagaaaattt
     3301 ctgttatccg gaaacgaaag agacaacgga taaatcaagc tggattgaaa aaagtgtgca
     3361 aatggtggtg gataaagcgg ttgaacttta tcgtgattat ttacaagcgg ttcaaaaacg
     3421 ggagaaaaat ccagattcag tgagtgtgaa agcgattttt gagaaatttc aaaaagtccg
     3481 aattttatca gccttaaggg taagtgaact gggggttgaa cgtcttaata tggcgattgc
     3541 ggaaggcttg cggaaagcta gcttagtgaa atttaatcat agccgagacc gctatatcgg
     3601 aaaaccgatt ttaatgacag aaaatatgcc gataaatagc atagccagcg gtgatgtcgg
     3661 catcattttg ccggacgaaa atggagaaag ccgagtttat tttgattcct tacaggaaaa
     3721 tagagaacac catagtttgc ctctaggtag ggtttcaaat tatgaagcag cttatattat
     3781 gactgtgcat aaatcgcaag gttctgaatt tgaacatacg gtattggtgt tgccccattc
     3841 gatttccccg gtgctgacga aagagttgat ttacaccgca atcacaaggg caaaagataa
     3901 atttacctta tttggtagcg aaaaagtttg gaaatacagt gttggtgcca aaaccgaaag
     3961 acaaagtggg ctaaaagaac aaataagagg caagtttagc taaattaata ctcgttttgc
     4021 cgtaaaaata cgcaagcggt catttttttc aaatttttta caaaaaccgc ttgcgttgtt
     4081 tttgtttttc cctataatac gcagcacaca acgacgcact gttgtgaacg atttaaaatt
     4141 g
//
LOCUS       DANLRK010000190         4130 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.190, whole genome shotgun sequence.
ACCESSION   DANLRK010000190 DANLRK010000000
VERSION     DANLRK010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4130
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.190"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            53..1054
                     /gene="pdxA"
                     /locus_tag="PXA33_002305"
     CDS             53..1054
                     /gene="pdxA"
                     /locus_tag="PXA33_002305"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HDL5121603.1"
                     /translation="MKPILGITMGDAAGIGPEVIIKALANQRIYELAHPVVIGDRKII
                     QRALPIVHSELKLRILNNLDNFVAEYGVIDILDLNNLPEDLPFAQVDPRAGKAAYEYI
                     EEAVNLAMGGKIHAIVTAPLNKEALHAGGKPFPGHTEILATLSNTKDYSMMLTSEKLN
                     VIHVTTHVQLRKACDLITKERVATVIDLAEENARILGFKEPRIAVAGLNPHSGENGMF
                     GDEDQKEIVPAIQEAQAKGFNVTGPVPPDTLFHRAANLNEFDIVVVMYHDQGHIPIKL
                     LGFDTGVNVTVGLPFIRTSVDHGTAFPIAGKGTADSRSMTEALYLGAKMANIKYATH"
     gene            1065..2063
                     /locus_tag="PXA33_002306"
     CDS             1065..2063
                     /locus_tag="PXA33_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HDL5121604.1"
                     /translation="MSTQTSKSIVPIYDGMNKIPGGAMVIPLILGSIIGTFFPEFLNL
                     GSFTTALFKSSAGPLIALLIFATGMQITVRSSGPVLAHTGVVLLFKTIIPALLVVILG
                     LWVGNDGVMGVSLLAMLTVVANSNGGIWLAFTGKYGDYRDKGAYVASAVNDGPFFVLL
                     FLGVSGLADIPLSLMLAAVIPLIIGMVIGNLDPKWTELMKPTGAIVIPFFAFALGTGI
                     NLQSIITGGATGIILGIVASLFTGFLVFIGYKTILRRGNQSGIGFAAGTTAGNAIITP
                     EIVAQADPTLAPFVQTATAQIAAAVLVSAILAPLFAAWVLKRQGGLEQDTIKGENL"
     gene            2060..3286
                     /locus_tag="PXA33_002307"
     CDS             2060..3286
                     /locus_tag="PXA33_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HDL5121605.1"
                     /translation="MKETLLVIADDLTGANDTAVMFAESGFDTVLKTKVSALAQIHPD
                     KAQVISVSTDSRAIGEKAKELTQIAISNAIQNSIGQIYLKIDSTMRGSVKYQIEGAIK
                     AWAGLYPDVKAIICPAYPEMGRTIEAGHLYVNGVPVNETASGKDPICPVHSSSMQKLL
                     PDAQVIPCDTVDNLQASIENSEYKQIVIDAKTHEDLSMIAQVINHIGNKIIPVGSAGL
                     AQKLKNIQNQQQPERNLKLGRALIVVTSIHETSQLQVDEYISTLGGKSIVFNPSPSQL
                     INYSQSEHSLKLQLNALIRFSRDHVIIRANPAKVVNAKSANITETSKEISQHLADLSK
                     FCLDNEKFDSLILFGGDGAAALLDQMNVTEMNVQYAVVPGVPLCSIEKGNYSGITVMT
                     KSGGFGNNTLLSNIMN"
     gene            3394..>4130
                     /locus_tag="PXA33_002308"
     CDS             3394..>4130
                     /locus_tag="PXA33_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL5121606.1"
                     /translation="MSKNRLEQILLFLKNHNIATVEQLVKVTEASPATIRRDLIKLDE
                     EGVVIRTHGGVSLNQFIPFQPTTNEKQFKQIVEKERIAEYAVSLISPGNSILLDAGTT
                     TLCIAKKIAHMPLRVITTDLHIALLLSEYKQIELIITGGRVDNSSQSCIGNHSLTLLE
                     NINPDIAFVSCNSWSLEKGVTAPTEEKANLKRMLQQNAKQKVLVADSSKYGKYSLFRA
                     TQLDNFTQIITDKGLPIEYSQALSSND"
BASE COUNT         1351 a          733 c          771 g         1275 t
ORIGIN      
        1 tcatataagc tatacttcgt tcaaaatgat taatttcaat caaggagtaa atatgaaacc
       61 tatattggga attacgatgg gagatgccgc cggtatcgga ccagaagtta ttattaaagc
      121 attagctaat caacggattt atgagttagc acaccctgtt gtgatcggtg atcgaaaaat
      181 tatacaacga gccttaccta ttgttcattc agaattgaaa ttaagaattc tcaataatct
      241 agataacttt gtagccgaat atggcgttat tgatatttta gatttgaata acctgcctga
      301 agatttgcct tttgctcaag ttgatccgcg tgctggaaaa gcagcttatg aatatattga
      361 agaggcagtt aatttagcaa tgggaggaaa aatccacgct attgttacag caccactgaa
      421 taaagaagcc ctacacgcag gtggtaaacc atttcctggc catacagaga tccttgcaac
      481 cctcagtaat actaaagatt actcaatgat gctaaccagt gaaaaactca atgttattca
      541 cgttactact cacgtccaac ttcgtaaagc ctgtgattta attaccaaag aacgagttgc
      601 tacagttatt gatttagcag aagaaaatgc tcgcattctt gggtttaaag aacctcgtat
      661 tgcagtcgct ggtttaaatc ctcattcagg tgaaaatgga atgtttggcg atgaagatca
      721 aaaagaaatt gttcccgcaa ttcaagaggc tcaagccaaa gggtttaatg ttacagggcc
      781 tgtccctccg gatactttat ttcaccgtgc agctaaccta aatgaattcg atattgttgt
      841 tgtgatgtat cacgatcaag ggcatatccc tatcaaatta ttaggctttg atacaggtgt
      901 aaacgttact gtcggtcttc catttattcg aacatctgtc gatcacggaa ctgctttccc
      961 aatagcaggc aaaggaacag cagatagtcg tagtatgact gaagcactct atttgggtgc
     1021 aaaaatggca aatatcaaat atgcgacaca ctaaggagat taatatgtct actcaaacat
     1081 ctaaatccat cgtcccgata tatgatggta tgaataaaat tcctggcggt gcaatggtaa
     1141 taccgctaat tcttggttct attattggta cattctttcc tgaattttta aatttaggtt
     1201 catttacgac ggctttattc aaatcatcag caggaccact tattgcacta ttaattttcg
     1261 ctacaggtat gcaaattacc gtgagaagtt ctggacctgt acttgcccat accggtgttg
     1321 tattactatt taaaactatt attcccgctc ttttagttgt gattttagga ttatgggtag
     1381 gtaatgatgg tgttatgggc gtttctttat tagctatgct aactgttgtt gcaaattcaa
     1441 atggtggtat ttggttagcg tttacaggga aatatggtga ttatagagat aaaggagctt
     1501 atgtcgcttc ggctgttaat gatggacctt tctttgtttt actattttta ggtgtatcag
     1561 gattagcaga tattcctctt tcattaatgc ttgcagctgt tattcctctt attattggta
     1621 tggttattgg taacttagat cctaaatgga cagagttaat gaaaccaaca ggtgcaatag
     1681 tcattccttt ctttgctttt gcattaggaa caggaatcaa tctgcaatct attattactg
     1741 gaggagcaac aggaattatt ttaggtatcg tagcttctct ctttacaggt tttcttgtat
     1801 ttatcggtta caaaacaatt ttaagacgtg gtaatcaatc aggaatcggg tttgcagcag
     1861 gaactactgc tggaaatgcc attattacac ctgaaattgt tgctcaagca gaccctacat
     1921 tagcaccttt tgttcaaact gcgactgcac agattgccgc agctgtatta gtttcagcaa
     1981 tcttagctcc attatttgca gcgtgggttt taaaacgtca aggtggctta gaacaagaca
     2041 ctataaaagg agaaaatcta tgaaagagac actcttagtt attgctgatg accttactgg
     2101 tgcaaatgat accgcggtta tgtttgctga atcaggattt gacactgtct taaaaactaa
     2161 agtttcggca ttagcccaaa ttcatcctga taaagcccaa gtaatttctg tttctacaga
     2221 ttcaagagca attggagaga aagcaaaaga gctgactcaa attgcaatta gcaatgctat
     2281 tcaaaatagt atcgggcaaa tttaccttaa aattgattcc acaatgagag gctctgttaa
     2341 atatcagatt gaaggtgcta ttaaagcttg ggctggttta tatcccgatg ttaaagcaat
     2401 tatttgtcct gcatatccag aaatgggaag aactatagaa gctggtcatt tatatgttaa
     2461 tggtgttcct gtaaatgaaa ctgcttcagg aaaagatcct atctgccctg tgcattcatc
     2521 ttctatgcaa aaactattgc ctgatgctca agtaatcccg tgtgatacgg tcgataattt
     2581 gcaggcatct attgaaaata gtgaatataa acagatcgtt attgatgcta aaacgcacga
     2641 agatctctca atgattgccc aagtcataaa tcacataggt aataaaatta ttcctgttgg
     2701 ctcagccgga ttggcacaga agttgaaaaa tatccaaaat caacaacaac cagagcgaaa
     2761 tcttaaatta ggtagagcat taattgtcgt aacctcaatt cacgaaacaa gccaactgca
     2821 agttgatgaa tatatttcaa ctttaggagg aaaatctatt gtatttaatc cttcgccttc
     2881 acagttgatt aactactcgc aatcagaaca ttcactgaaa ctacaattaa acgctctaat
     2941 acgttttagt agagatcatg ttattattcg ggcaaaccct gcaaaagttg taaatgcaaa
     3001 atctgccaat atcacggaaa cttctaaaga aatttctcaa catttagctg atttaagtaa
     3061 attctgcctt gataatgaaa aatttgatag ccttatatta tttggtggag atggtgcagc
     3121 agcattatta gatcaaatga atgtaacaga gatgaatgta caatatgcgg tagtaccggg
     3181 cgttccttta tgctccattg aaaagggcaa ctactcaggc atcactgtaa tgactaaatc
     3241 tggaggattt ggaaacaaca cactcttaag caatataatg aactagtaac aacaaagaac
     3301 ctaaagcaac aatgttttag gttctttaat tttaactatc ttatttttaa aaaattactt
     3361 atagtaaaag cagttaaaaa ttagagagtt tatatgtcta aaaaccgtct agaacaaatt
     3421 ttactattcc taaaaaacca caatattgct accgtagagc aactggtaaa agtgactgag
     3481 gcttctcctg caactatccg ccgtgattta ataaaattag atgaagaagg cgtcgtgatt
     3541 cgcacacacg gaggggtcag cttaaaccaa tttataccgt ttcaaccaac aacaaatgag
     3601 aagcaattta agcaaattgt cgaaaaagaa cgcatagctg aatatgccgt ttcgcttatt
     3661 tcaccgggaa attctatttt attagatgct ggcactacaa cgctatgcat tgcgaaaaaa
     3721 atcgctcata tgccacttcg agtgattaca actgatctac atattgcttt attactctct
     3781 gaatataagc aaatagaatt aattattaca gggggacgag tagataatag tagtcaatct
     3841 tgtattggta atcatagttt aactttatta gaaaatatta atcctgatat tgcttttgtg
     3901 agctgtaatt cttggagctt agaaaaagga gttacagcac ctacagaaga gaaagctaat
     3961 ttaaaaagaa tgctccaaca aaacgccaaa caaaaagtac tggttgccga tagcagcaaa
     4021 tatggaaaat actctttatt tcgtgcaact caactcgata actttacaca aattatcaca
     4081 gataaaggac tacctattga atatagccaa gccttgtcaa gtaatgattt
//
LOCUS       DANLRK010000191         4000 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.192, whole genome shotgun sequence.
ACCESSION   DANLRK010000191 DANLRK010000000
VERSION     DANLRK010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4000
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.192"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            86..289
                     /locus_tag="PXA33_002309"
     CDS             86..289
                     /locus_tag="PXA33_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDL5121607.1"
                     /translation="MFTQTLLAQRTGLSRVTVRKIMNGDPTVAIGYYLMVLGVLGLEQ
                     DFTDVGKDDELGRKLQDIELLRK"
     gene            289..1530
                     /locus_tag="PXA33_002310"
     CDS             289..1530
                     /locus_tag="PXA33_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HDL5121608.1"
                     /translation="MMNKRIYVFADWETFSQPELVGVLSSDVVRGKENFRFSYDNEWL
                     SSPYVQQIDPNLYLYPGEQYSTDTQNFHIFLDSCPDRWGRLLMKRREAALASQENRKP
                     KLLIESDYLLGVHDMNRMGALRFKTELNGPFLDNNTRLAAPPLSSLRELEFAAIQLES
                     SDNVDDPDYLKWLHMLISPGSSLGGARPKASVIDELNQLWIAKFPSRYDDYDIALWEY
                     LAYRLAIQAKIEMATCRIERFNQEHHTFLTQRFDRHCGKRRHFTSALTQLGYYDGDYE
                     ASYLEIAQFLTESGSNTRIDLAQLWRRIVFNIAISNTDDHLRNHGFIYAQEGWILSPA
                     YDLNPVPLSQGLHLNITDSDNRLDYQLAFDVADFFQLSKQQAVQIYDEVLKEINNWQN
                     VAKEIGIPRQEQEQMKFAFNV"
     gene            complement(1934..3031)
                     /locus_tag="PXA33_002311"
     CDS             complement(1934..3031)
                     /locus_tag="PXA33_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HDL5121609.1"
                     /translation="MFYSSNPLIKHKTGLLNLAEELGNISQACKVMGMSRDTFYRYQQ
                     AVEQGGVEALLNQNRRVPNIKNRVDESTEKAVVQFALDNPAFGQVRVSNELRKQGIFV
                     SAGGFCLCRWFLSLQVVFVSAGGVRSIWLRHHLANFKQRLTALEKLVAEQGIILSEPQ
                     IQALERKKEDEIACGEIETAHPGYLGSQDTFYVGNIKGVGRIYQQTFIDTYSKVAFAK
                     LYTMKTAITAADMLNDKVLPFFESQRLPMLRVLTDRGSEYCGKVENHDYELYLAINDI
                     EHTKTKVKQPQTNGICERFHKTILQEFYQVAFRKRIYMDLEALQADLDEWLMYYNHHR
                     THQGKMCCGRTPMETLVDGKRIWAEKNLSSN"
     gene            3361..>4000
                     /locus_tag="PXA33_002312"
     CDS             3361..>4000
                     /locus_tag="PXA33_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HDL5121610.1"
                     /translation="MYLMYVDESGSTTDPTQKYFVLSGIAVFERNTHWIEKKMNVIAS
                     QFCQYSEGSDPYFYELHGSPMRSGKSEWRGIPMSERINAIQDCLKLVQSNNIRIFAAV
                     IEQGYSSGQDTITECFEQISSRFDMYLSRLHAQGNTQRGISIFDKSSTEKSIQALART
                     FKNSGHTYGKLRNFSEVPLFLDSKSSRLIQLADLVSYAIFRHYEHNDSSFYDI"
BASE COUNT         1252 a          755 c          755 g         1238 t
ORIGIN      
        1 aacaaaatca ccaactctgt ttcctaagta ccaaaatgcc ttgcttcaac ttggagaaaa
       61 tattaggcta gcctcaaaaa ggagaatgtt tactcaaaca ttattggctc aaagaactgg
      121 gcttagccgt gtaacagtga gaaaaattat gaatggtgat ccaactgttg cgattggata
      181 ctacctaatg gttttaggtg ttctcggtct agaacaagat ttcactgatg ttggaaagga
      241 tgatgagtta ggacgtaaat tgcaagatat tgaattatta aggaaataat gatgaataaa
      301 cgaatttatg tgtttgcaga ttgggaaact ttttcacagc ctgaattagt tggggtgctt
      361 tcttctgatg tagtgagagg caaagaaaat ttccgtttta gctacgataa cgagtggtta
      421 agttcaccct atgtacagca gattgatcct aacttatacc tttaccctgg agagcaatac
      481 agtacagata cacaaaattt tcatatcttt ttagattctt gccctgatag atggggtaga
      541 ttgttaatga aacgtcggga ggcagctcta gctagtcaag agaatcgtaa acctaagtta
      601 ctaatagaaa gtgattacct tttaggcgta cacgatatga atagaatggg ggctttacgt
      661 tttaaaactg aattgaatgg tccatttttg gacaataata ctcgtcttgc agcaccacca
      721 ctgtcttcct taagagaatt agaatttgca gcaatacaac ttgaaagcag tgataatgtg
      781 gacgatccgg attatttaaa atggcttcat atgttgatat caccagggtc gtcactaggc
      841 ggagctagac caaaagcttc ggtaatagat gaactaaatc aactatggat tgccaaattt
      901 ccaagtcggt acgatgatta tgatattgct ctttgggaat atttagctta tcgactggct
      961 attcaagcca aaattgaaat ggctacttgt agaattgaac gctttaacca agaacaccat
     1021 acttttttga cacaaagatt tgatcgacat tgtggaaaac gtcgccattt tacgtcagca
     1081 ttaacccagc tgggttatta tgacggagac tatgaggcaa gctaccttga gattgctcaa
     1141 tttttaacag aaagtggttc aaatactcga attgacttgg ctcaactatg gagaagaatt
     1201 gtattcaata ttgctatttc gaatactgat gatcatttgc gtaaccacgg atttatctac
     1261 gctcaagagg ggtggatatt atctcccgca tacgatttaa acccagtacc actatctcaa
     1321 ggattacatt taaacataac agacagcgat aaccgattgg attatcagct cgcctttgat
     1381 gttgctgact tttttcaatt atctaagcaa caagccgtac agatttatga tgaagtactg
     1441 aaagaaatta ataattggca aaatgtcgca aaagaaattg gtattccacg ccaagagcaa
     1501 gagcagatga aatttgcatt taatgtatag taattgactt gtggaaccaa tcatcgtagt
     1561 ctatgttcta aaaaagtagc gttaccttaa taacgcaatg agttgttggc tcattgtcaa
     1621 tctgatatta atctaggatg taaaggccta gcggtggcag gcaccagaaa gacctgatac
     1681 taatctaggg tataaagacc tagcggtggc agacaccaga acaaaccaac atcttataaa
     1741 accattagta acacttgact tttttctcaa ccatatccgc aaggaagcgt agccttaaaa
     1801 acgcaaatgt aggaaactaa tctaggcata tataatgcct agcagatggc agacatcaga
     1861 aaaactaaca tttactgtag aagatcaaac ctaatctgac agtcccccgt ttaaaattac
     1921 cgtgtctgtc agattaattt gagcttaaat tcttttctgc ccaaatgcgt tttccatcaa
     1981 ctaatgtctc catcggtgtt cttccacagc acatttttcc ttgatgggtt cgatgatggt
     2041 tataatacat taaccattca tctaagtcag cttgcaatgc ctctaaatcc atataaatcc
     2101 gttttctgaa tgccacttgg tagaactcct gcaaaatcgt tttgtgaaaa cgctcacaga
     2161 tgccgtttgt ttgtggctgt ttcactttgg tttttgtgtg ctctatgtca ttgatagcca
     2221 aataaagctc ataatcgtga ttctcaactt tcccacaata ctcactgcct cggtctgtca
     2281 gcactcgtaa cataggtaac ctctggcttt caaagaatgg taatactttg tcatttagca
     2341 tatcagcagc agtgatagcc gtcttcattg tatacagttt tgcaaatgcc actttactat
     2401 aagtatcaat aaacgtttgc tgataaatac gacccacacc tttaatattg ccgacataaa
     2461 aggtatcttg tgaccctaaa tagccggggt gtgccgtttc aatttcacca caggcaatct
     2521 catcttcttt cttccgctcc aaggcttgta tctgtggctc actcagaata atgccttgct
     2581 ctgccaccaa tttttcgagg gcagttaagc gttgcttaaa attagctaag tgatgtctca
     2641 accaaatgga acgtacacca cctgcagaga caaaaaccac ctgcagagac aaaaaccacc
     2701 tgcagagaca aaaaccacct gcagagacaa aaatcccctg tttacgcagt tcattactca
     2761 ctcgaacttg accaaaagca ggattatcta aagcgaattg tacaaccgct ttttctgtgg
     2821 attcatccac acgattttta atgttgggaa cacgtcgatt ttgatttagt aatgcctcaa
     2881 caccgccttg ttcgacagcc tgttgatagc gataaaaagt atctcgactc atccccatta
     2941 ccttacaggc ttgtgagata ttacccagtt cttctgctaa atttaataaa ccggtcttgt
     3001 gtttaataag tggattgcta gaataaaaca tgagagtttc ctttttgttt agattgattt
     3061 ttgacactca tattctaaac gggaaactct cactttttag agtgaattgt cagatcaagt
     3121 ctgatcttct acagacgatc caggtcttac acattctttc aagtttgtaa agcctgaaaa
     3181 ttatttttta tacttgattt ttgtcaaaaa gtctctacac tatatggcag ggtcagtttt
     3241 cgctgtttaa cggtgtttaa tgagattttc attactggct ctccttaata gaaatttatg
     3301 gaagagctat ctgatagagt aatctagcga tagctctttt tcattgaaca aagaggctat
     3361 atgtatctta tgtatgttga tgagtctggt tctacgacag atcctactca aaaatatttt
     3421 gtattatcag gtatcgctgt atttgaacga aatactcatt ggattgagaa aaaaatgaat
     3481 gtgatagcct ctcaattctg tcaatattct gaagggagtg atccatattt ctatgagtta
     3541 catggttcac caatgagatc tggaaagagt gaatggagag gcattccaat gagtgagcgt
     3601 attaatgcca ttcaagattg cttgaaatta gttcaaagta ataatattcg tatatttgca
     3661 gcagttatag agcaagggta ttctagtggt caggatacta ttacagaatg ttttgagcaa
     3721 atatcatctc gatttgatat gtatctgagc cgtttacatg cccaaggtaa tacccaaaga
     3781 ggcatttcta tctttgataa atcatcgact gagaagtcaa tccaagcact tgcaagaaca
     3841 ttcaaaaata gtgggcatac ttatgggaaa cttagaaatt tctcagaagt tccattattt
     3901 cttgactcaa agtcatcgag attaattcag ctagcggatc tagtttccta tgcgatattt
     3961 agacactatg aacacaatga tagctcattt tacgatatta
//
LOCUS       DANLRK010000192         3980 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.193, whole genome shotgun sequence.
ACCESSION   DANLRK010000192 DANLRK010000000
VERSION     DANLRK010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3980
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.193"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            43..1518
                     /gene="mepM"
                     /locus_tag="PXA33_002313"
     CDS             43..1518
                     /gene="mepM"
                     /locus_tag="PXA33_002313"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HDL5121611.1"
                     /translation="MAVFLLAVFSLFVDIVLALKDQSSINNTSSQEDIIAIDKQEKYQ
                     SINTNLAENNTLHQEQHSSFINYVTDLLTQSALVIEHDDEIVDDQTATSYEDELVGQD
                     DEVEEGIETVENQVPGEKLPEEAEKALEDLLDVADQALRIQNQFSYTVARGDKLKDVL
                     EQSGLTASVAKNLIKQHSALAQLEPGQQFYWVLDNDGELEYMNWLVSEKEERVYERKE
                     NGKFSFQRFEKKGEWKLDVVRGTIQNSFAVSLKAVGLSDRQINQLAVGLQSQIATNKL
                     KKGDRFAILIKREYIDGKVTDLGNVEGIHVISSKKSYYAIQADNGRYYSRYGETLSGG
                     FARHPLLFAARVTSSFNPKRRHPITGRISPHKGVDYGVPIGTPIIAPSDGVVEHVAYQ
                     ARGAGRYIRIKHGHITTVYMHLSKTLVKPGQSVKKGERIALSGNTGGSTGPHLHYEFH
                     INGRPVNPITVKLPGSNSGMGNKERQAFLNKAKNVESRLKM"
     gene            1528..2211
                     /gene="dsbC"
                     /locus_tag="PXA33_002314"
     CDS             1528..2211
                     /gene="dsbC"
                     /locus_tag="PXA33_002314"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HDL5121612.1"
                     /translation="MKKNLITLGLMCLSTITFASDAKLKSQLEKIGATDVKISDSPLT
                     GFKMATSNEGVVYISQDGRYVIQGKVFELKNGKAIDITNQGLLAELNSYTNEMIVYPA
                     KNEKHVITVFMDITCHYCHLLHTKIKEYNDLGITIRYLAFPRGGMNTQTAKQMEAIWT
                     SADKVKALNDAEDGKLPTQIKTPDIVKKHYQLGIKFGVTGTPNMVTSEGELIGGYVEP
                     KELAKMLSE"
     gene            2296..>3980
                     /gene="recJ"
                     /locus_tag="PXA33_002315"
     CDS             2296..>3980
                     /gene="recJ"
                     /locus_tag="PXA33_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HDL5121613.1"
                     /translation="MEKIIKRRPLLTTEIVSTNPLLNRIYQSRGIKNTQELEHNLKNL
                     HRPQQLANIEQAVDLLLQAFREKSRIIIVGDFDADGATSTSLALIALRQLGFDKVDYL
                     IPDRFSQGYGLSVAVAEMVLAKGADLVLTVDNGISSTEGIALLKSHQIQVLVTDHHLP
                     PESLPNADALVNPNLAFCEFPSKSLAGVGVIFYVMMALRSRMREQHLFENKPEPNFAE
                     LLDLVALGTVADVVPLDQNNRILVHQGLQRIRSGHCRLGIKALAEVGKRDLSTLQAAD
                     LGFAIAPRLNAAGRLENMSLGVELLICENMELARRLALDLDSLNQTRKEFEQEMKAEA
                     LSICANLPSLAQSEQAHGIVLYQADWHQGVIGILASRIKDQFNRPVIAFAQESEESEY
                     LKGSARSINGVHMRDLLEHIDMRHPDLIEKFGGHAMAAGLTIHQSKLDLFKQIFDQSI
                     NAIIEPEQLQGIIYTDGELNALEFTLANAEMIRQGGPWGQHFPEPSFEGEFSILQQKL
                     LAGKHLKLMLQSEKGQLFDAIWFNVDVRAFPDLSIKKARLVYRLDINEFRGEKS"
BASE COUNT         1331 a          642 c          876 g         1131 t
ORIGIN      
        1 attttagcga gagatcgtaa gcgacataaa aaacatttta agattgcggt tttcttatta
       61 gcggtattta gtctatttgt tgatattgtt ctcgcactaa aagatcaatc ctcaatcaat
      121 aatacatctt cacaggaaga tattattgcc attgataaac aggaaaaata tcagagtatc
      181 aacactaatt tggcggaaaa taatactctc catcaggagc aacattccag ttttattaat
      241 tatgtgactg atttactgac tcagtcggca ttagtaatcg aacatgacga cgaaattgtt
      301 gatgatcaaa cagcgacttc ttatgaagat gaattagttg ggcaagacga tgaagtagaa
      361 gaagggattg aaacggttga aaatcaagtt cctggggaaa aactcccaga agaagctgaa
      421 aaggcattag aagatttact tgatgtggct gatcaagcac tacgaattca aaaccaattc
      481 agttataccg tagctcgagg tgataaactt aaagatgtat tagagcaatc aggcttaact
      541 gcatccgttg cgaaaaattt aattaagcaa cattcggcat tagcacaatt agaaccgggt
      601 caacaatttt attgggtatt agataatgac ggtgaattag aatatatgaa ctggctggtt
      661 tccgaaaaag aggaacgggt ttatgagcga aaagaaaatg gtaaattttc ttttcaaaga
      721 tttgagaaaa aaggggaatg gaagttagat gttgtaagag gaactattca gaatagtttt
      781 gcggttagct taaaagccgt tggcttatca gaccgccaaa ttaaccagct agcggttgga
      841 ttgcagtcac aaattgcgac gaataaatta aaaaaagggg ataggtttgc aattctgatt
      901 aagcgggaat atattgacgg caaagtaacg gatttaggaa atgtggaagg aatccatgta
      961 attagcagta aaaaaagtta ttatgctatt caagcagata atggacgcta ttatagtcga
     1021 tatggtgaaa cattaagtgg aggatttgct cgtcatccat tattatttgc agcaagagta
     1081 acttcttctt tcaaccccaa acgcagacac cctattacag ggagaattag tccgcataaa
     1141 ggggttgact atggcgtgcc aataggtact ccgatcatcg caccaagtga tggtgtcgta
     1201 gagcatgtgg catatcaagc ccgtggagca ggacgttata ttcggataaa acacggacat
     1261 attacgactg tttatatgca cctcagcaaa acgttggtaa aaccagggca gagtgttaaa
     1321 aaaggggagc ggatagcatt atcgggtaat acaggcggtt caaccgggcc tcatttacac
     1381 tatgaattcc atattaatgg tcgaccggtc aacccgatta cggtaaaatt gcctggctca
     1441 aattcaggta tgggtaataa agaacgtcaa gcattcttaa ataaagcgaa gaatgtagaa
     1501 agtagattaa aaatgtaagg attctcaatg aaaaaaaatc taatcacttt aggattaatg
     1561 tgtttatcaa ccatcacgtt tgcttcagat gcaaaactaa aaagccagct tgaaaaaatt
     1621 ggagccacag atgttaaaat ttcagattct ccattaacag gctttaaaat ggcgacttca
     1681 aatgaaggtg ttgtatatat cagccaagac ggtcgttatg taattcaagg taaagtattt
     1741 gagctaaaaa atggtaaagc gattgatatt actaatcaag gattgttggc agaattgaat
     1801 tcatacacta atgaaatgat cgtatatcca gcgaaaaatg agaaacatgt gattacggtg
     1861 tttatggata ttacttgcca ttattgccat ttactacata ctaaaatcaa agaatataat
     1921 gatttaggta ttaccattcg ctatttggca ttcccgcgtg gtggaatgaa tacccaaaca
     1981 gcaaaacaaa tggaagctat ttggacatca gcagacaaag taaaagcact gaatgatgct
     2041 gaagatggta aattaccaac ccaaataaaa acgccggata ttgtgaaaaa acattaccaa
     2101 ttagggatta aatttggtgt gacaggtacc ccaaatatgg taacttcaga aggtgagttg
     2161 attggtggtt atgtagagcc taaagaatta gcgaaaatgt taagtgagta attcattaac
     2221 ttgaatacaa gcggtctttt tttgtaaaat ttttgcaaaa gaagaccgct tgttgtttca
     2281 ttaaagaaga gtattgtgga aaaaataatt aaaaggcgcc cattattaac aacggaaata
     2341 gtctctacaa acccgttatt aaatcgaatc tatcaaagtc gaggcataaa gaatacgcaa
     2401 gaactagagc ataatttaaa gaatttgcat agaccacagc aacttgccaa tattgagcaa
     2461 gccgttgact tgctattaca agcctttcgt gaaaaaagcc ggatcattat tgtgggtgat
     2521 tttgatgcag atggtgcaac cagtacatcg cttgccctta ttgcgttacg ccaattgggt
     2581 ttcgataagg tagattattt aattccggat cgtttttctc aggggtacgg attgagtgta
     2641 gcggttgctg aaatggtatt agcaaaaggg gcggatttag tcttaacggt agataatgga
     2701 atttcttcga ctgaaggcat tgctctgcta aaaagccatc agattcaagt tttggttacc
     2761 gatcatcatt taccgccaga gagcctaccc aatgcagatg cactggtaaa ccccaattta
     2821 gctttttgtg aatttccgtc taaatcttta gccggggtag gcgtgatctt ctatgtaatg
     2881 atggcattgc gttctcgtat gagagaacaa catttatttg aaaataaacc agagccaaat
     2941 tttgctgaat tactggattt agtcgcttta ggcacagtgg ctgatgtagt gccgttagat
     3001 caaaataatc ggatattggt gcatcaaggg ttacagcgaa tccgttcagg gcattgccgt
     3061 ttagggataa aagctttagc ggaggtgggc aagcgagatc tttctacttt gcaagcagca
     3121 gatttgggtt ttgctatcgc accacgtttg aatgcagctg gtcggttaga aaatatgtct
     3181 ttaggggttg agttactaat ttgtgaaaat atggaattag ctcgccggct tgcgcttgat
     3241 cttgattctc ttaatcaaac ccgaaaagag tttgaacaag aaatgaaagc agaagcactg
     3301 tcgatttgtg cgaatttgcc aagtcttgcg cagtctgaac aggcacacgg aatagtgtta
     3361 tatcaagcag attggcacca aggtgtaatt gggatcttag cttctcgcat taaagatcaa
     3421 tttaaccgtc ctgtcattgc ttttgcacaa gaaagtgagg aaagtgaata tctaaaaggt
     3481 tctgctcgct cgatcaacgg agtacatatg cgagatttac tggaacatat cgatatgcga
     3541 catccagact tgattgaaaa atttggtgga cacgcaatgg cggctggttt gactatccat
     3601 caatcaaaat tagatttatt taaacagata tttgaccaat ctattaatgc aattatcgag
     3661 cctgagcaat tacaaggcat catttatacc gatggtgagt taaatgcact agagtttact
     3721 ttagccaatg ccgagatgat taggcaaggt gggccttggg gacaacattt tccagaacct
     3781 agttttgaag gggagtttag cattctccaa caaaaattac tcgcaggtaa acatttaaaa
     3841 ttaatgttgc aatcagagaa agggcaacta tttgatgcaa tttggtttaa tgtggatgta
     3901 agggcgtttc ctgacttatc gatcaaaaag gcaagattag tttataggtt ggatattaat
     3961 gaatttcgag gagagaaatc
//
LOCUS       DANLRK010000193         3969 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.194, whole genome shotgun sequence.
ACCESSION   DANLRK010000193 DANLRK010000000
VERSION     DANLRK010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3969
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.194"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(118..1758)
                     /gene="pgi"
                     /locus_tag="PXA33_002316"
     CDS             complement(118..1758)
                     /gene="pgi"
                     /locus_tag="PXA33_002316"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013527751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HDL5121614.1"
                     /translation="MQKINPVQTFAWNALEQHKADNLSIPQLFAEDPNRFDKYSLRFE
                     DQLLVDFSKNAINEKTLTLLRQLANECGLESAKNAMFSGKKINRTENRAVLHVALRNR
                     ANTPIAVDGKDVMPEVNAVLAKMKDFCERVISGEWKGYTGKTITDVINIGIGGSDLGP
                     YMVTEALRPYKNHLNMHFVSNVDGTHIAEVLRKVNPETTLVLVASKTFTTQETMTNAL
                     SARKWLLDSAKDESAVAKHFVALSTNAKEVEKFGIDTANMFEFWDWVGGRYSLWSAIG
                     LSIALSLGFENFEQLLAGAHAMDKHFLITPIEKNIPTTLALVGIWNNNFLGAESEAIL
                     PYDQYMHRFAAYFQQGNMESNGKFVGRDGKPVTHQTGPIIWGEPGTNGQHAFYQLIHQ
                     GTKLIPCDFIAPAQTHNPLSDHHNKLLSNFFAQTEALAFGKSKETVEKEFLDAGKTLE
                     EVAEIVPFKVFSGNKPTNSILVQKITPYSLGALIAMYEHKIFVQGVLFNIYSFDQWGV
                     ELGKQLANRILPELETDEKINSHDSSTNGLINQFKAWR"
     gene            complement(1791..2039)
                     /locus_tag="PXA33_002317"
     CDS             complement(1791..2039)
                     /locus_tag="PXA33_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HDL5121615.1"
                     /translation="MLTPKNLEAIAQQLHNALPQSLKNVGNDLDEKFKQILQAQLARL
                     DVVTREEFDVQSQVLLRTREKLTELEKRLDELTQRTDE"
     regulatory      2163..2207
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            2211..2879
                     /gene="queC"
                     /locus_tag="PXA33_002318"
     CDS             2211..2879
                     /gene="queC"
                     /locus_tag="PXA33_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HDL5121616.1"
                     /translation="MTHQKPKAVVIFSGGQDSTTCLFQAIAEFGKENVEVVTFQYGQR
                     HSIELEKARWISNDLGVKQTLIDTSVIKAITTNALMDNNAEIQQQGSTPNTFVDGRNA
                     LFLLYTAIYAKGQGIQTIFTGVCETDFSGYPDCRDVFVKSMNVTLNLAMDYNFNIRTP
                     LMYLTKKETWALADKLGAFDYIRDHTHTCYLGVEGGCHDCPSCVLREKGLNEYLAERE
                     VSNV"
     gene            2872..3294
                     /gene="queD"
                     /locus_tag="PXA33_002319"
     CDS             2872..3294
                     /gene="queD"
                     /locus_tag="PXA33_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HDL5121617.1"
                     /translation="MFKIAKEFSFDMAHMLDGHDGKCQNLHGHTYKLQVEVAGELFQE
                     GAKRGMVMDYSDLKAVVKQHILEPMDHAFIYDLQSERESQVAQLLIDLNSKVYGIPTR
                     TTAEEMAKYMFEKLEKVGLPVSLIRLWETPTSYCEYSK"
     gene            3294..3920
                     /locus_tag="PXA33_002320"
     CDS             3294..3920
                     /locus_tag="PXA33_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HDL5121618.1"
                     /translation="MAELISNPRFPIVEIFESLQGEGFNTGMPAIFVRFGKCNLTCPW
                     CDTNYNQFEQWTLSDILAKVRSYSAKNVIITGGEPTIQPNLSLLLEQFKKEGYFLAIE
                     TNGLKEVPAQIDYIATSPKRMYQEKYQRRCIEFANEVRIVVDGEVQGFCEQIESQIKA
                     EHYYLSPCEVEGKMNLLETITQIGLLNQRPNKPKWQLSIQTHKIVGIE"
BASE COUNT         1189 a          740 c          844 g         1196 t
ORIGIN      
        1 agaatataga aaaaactgct tgtgtagaaa tggcgcttag ttcagtcctc agctaaaatt
       61 ttagaaaaaa agcatagaga ttttttacct ctatgctttt tattgtattg actatattta
      121 acgccaagcc ttgaattggt taattaaacc atttgtggag ctgtcgtggc tgttgatttt
      181 ttcatcggtt tcgagttcgg gtaaaatgcg gtttgcaagc tgtttgccta actccacgcc
      241 ccattggtcg aaactgtaaa tgttgaacag cacgccttgc acgaagattt tgtgttcata
      301 cattgcaatt aatgccccta agctgtacgg ggtgattttt tgcaccaaga tagagttggt
      361 cggtttgttt ccgctgaaca ctttaaacgg cacgatttca gctacttctt ccaacgtttt
      421 acctgcatct aaaaactctt tttcaactgt ttcttttgat ttaccgaaag ctagtgcttc
      481 agtttgagca aagaagttag aaagcagttt gttgtgatga tcgcttaacg gattgtgagt
      541 ttgtgccggt gcgataaaat cacaaggaat taattttgtg ccttgatgaa tgagttgata
      601 gaatgcgtgc tgaccgttag ttcccggttc gccccaaata atcggacctg tttggtgagt
      661 aaccggtttg ccgtcacgcc ccacaaattt accgtttgat tccatattgc cttgttggaa
      721 ataagccgca aagcggtgca tatattggtc gtaaggtaaa atcgcttctg attcagcacc
      781 taagaaattg ttattccaaa tgccgactaa agctaacgta gttggaatgt ttttctcaat
      841 cggcgtgatt aagaaatgct tatccattgc gtgagcacca gcaagtagct gctcgaagtt
      901 ctcaaaacct aatgaaaggg caatcgaaag cccgatggca gaccataatg agtaacgacc
      961 gccaacccaa tcccagaatt caaacatatt agcggtatca ataccgaatt tttccacttc
     1021 tttggcattg gttgaaagtg ccacgaaatg ttttgctacc gcagattcat ctttggcaga
     1081 atcaagtagc catttgcgag ctgaaagggc attggtcatc gtttcttgag tggtaaaggt
     1141 ttttgaagcc actaacacta acgtggtttc aggattaact ttacgcaaca cttcagcaat
     1201 atgcgtgccg tccacgttag acacaaagtg catattaagg tgatttttat aagggcgtaa
     1261 tgcctctgtt accatataag gacctagatc cgagccacca ataccgatat tgatcacatc
     1321 agtaatcgtt ttgccggtat agcctttcca ttcgccggaa ataacacgct cgcagaaatc
     1381 tttcattttc gctaacacgg cattcacttc cggcattaca tctttgccat cgactgcgat
     1441 tggtgtattg gcacggttac gcagtgcaac gtgcaatacg gcacggtttt cagtgcggtt
     1501 gatttttttg ccggagaaca tcgcattttt tgctgattcc aaaccacatt catttgccag
     1561 ttggcgaagt aaggttaagg ttttttcgtt gatcgcattt tttgaaaaat cgactaataa
     1621 ttgatcttca aaacggaggg aatatttgtc gaaacggttt ggatcttcgg caaaaagttg
     1681 aggaatagaa aggttgtctg ctttatgttg ctctaaggca ttccaagcaa aagtttgcac
     1741 agggttaatt ttttgcatct gagtttcctt atactaaatt aaaaaatcga ttattcatct
     1801 gttctttgag tcagttcatc aagacgtttt tctaattcgg ttaatttttc acgagtgcgg
     1861 agtaacactt gagattgaac atcaaattct tcacgagtga caacatcaag acgggctaat
     1921 tgggcttgaa ggatttgctt aaatttttca tctagatcat taccgacatt ttttaagctt
     1981 tgcggtaagg cgttatggag ttgttgagca atcgcttcta agttcttcgg cgtgagcata
     2041 tttaaattcc ttactataaa tgagtatccc aattttattg aattttgggt ttaagagaaa
     2101 gcaaattctt tgtaaaatat cacctttcgc aaaatacaag caaaaaagac cgcttgtatt
     2161 ttttatttgt ggttcgtaaa cctcccacac aaaaaaacta aggacacaaa atgactcatc
     2221 aaaaaccaaa agccgttgtg attttttccg gcggtcaaga ctctaccact tgtttattcc
     2281 aagctattgc tgaatttggc aaagaaaatg tggaagtcgt tacctttcaa tatgggcaac
     2341 gccattcgat tgaacttgaa aaagcacgct ggatttctaa cgatttaggc gtcaaacaaa
     2401 cgctgattga tacttcggtg attaaagcga ttactaccaa tgcgttaatg gataataatg
     2461 ctgaaattca acaacaaggc agcacaccca atacctttgt ggatgggcgt aatgccttat
     2521 ttctgcttta taccgccatt tatgccaaag ggcagggcat tcaaactatt tttaccggcg
     2581 tgtgtgagac cgattttagt ggttaccctg attgccgaga tgtatttgta aaatcaatga
     2641 atgtaacgct aaatttagcg atggactata atttcaacat tcgcacgcca ttgatgtatt
     2701 taactaaaaa agaaacttgg gcattagcag ataagctggg tgcttttgat tatattcgag
     2761 accataccca cacctgttat ttaggcgttg aaggtggttg ccacgactgc ccgagttgcg
     2821 tattgcgaga aaaaggcttg aatgaatatt tagcagaaag ggaggtaagc aatgtttaaa
     2881 attgctaaag aatttagttt tgatatggct catatgctag atggacacga tggcaaatgc
     2941 caaaacttac acggacacac ctacaaattg caagttgagg tggcaggtga gctatttcaa
     3001 gagggagcta aaagaggaat ggtaatggac tattcggatc taaaagcagt ggtaaaacaa
     3061 catattttag agccgatgga tcacgctttt atttacgact tacagagtga gcgtgaaagc
     3121 caagtagcac aattattaat tgatttaaac tccaaggttt atggtatccc aactcgtacc
     3181 acagccgaag aaatggcgaa atatatgttt gaaaaattag agaaagtggg cttaccggtt
     3241 agcctaattc gcttgtggga aacgcctact tcttattgtg agtacagcaa ataatggcgg
     3301 agttaatttc aaaccctcgt tttccgattg ttgagatctt tgaaagctta caaggggaag
     3361 gctttaatac cggaatgccg gctatttttg tacgttttgg caaatgcaac ctcacttgcc
     3421 cgtggtgcga taccaattac aatcaatttg agcaatggac gttaagcgat attcttgcca
     3481 aagtaagaag ctattctgcg aaaaatgtga ttattaccgg aggcgagcca acgattcagc
     3541 ctaatttatc gttattatta gaacaattta aaaaagaggg atatttttta gcgatcgaaa
     3601 ccaacggctt aaaagaggtt ccggcacaaa ttgattatat tgcgaccagc ccaaagcgaa
     3661 tgtaccaaga aaagtatcaa cgccgttgca ttgaatttgc gaatgaagtc cgtatcgtcg
     3721 ttgatggaga agtacagggt ttctgtgagc aaatagaaag ccaaattaaa gctgagcatt
     3781 actatctttc accttgtgag gtagagggaa aaatgaactt gctggaaacc atcacccaga
     3841 ttgggttatt aaatcagcgt ccaaataagc ctaaatggca gctaagtatc caaacgcata
     3901 aaattgtggg gattgaataa gtagaagtta gaagatgatt tgtaaaagat cagacttgat
     3961 ctgacaatt
//
LOCUS       DANLRK010000194         3949 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.195, whole genome shotgun sequence.
ACCESSION   DANLRK010000194 DANLRK010000000
VERSION     DANLRK010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3949
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.195"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(334..1428)
                     /locus_tag="PXA33_002321"
     CDS             complement(334..1428)
                     /locus_tag="PXA33_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HDL5121619.1"
                     /translation="MKKTTLAILASALIANSALAITIYDHKETGTQIEFIGSARIKWE
                     STSDKTSPVNGKATRNHVNHAVDNNGSRFGFRLTQQVGHGFYALGRVEWRMRGDSSSQ
                     HNFDHIYDHQLYAGIGHKQYGELTYGNMTNIADEVKQTDLPNTLSLSDGLLTGSSRRT
                     LQYVYKGIEGLKLGAFYGGNSQRGNNGLDLVNKRKDIWGAAAIYNYKIDDNQSLKLAT
                     GATRERSEQSIGNYERIALAFGTAYTYGKTTLGLDLERRETEDQNAIGHKRIEKEVRT
                     VLLHRLTDQWNAYTMYAYKENKLNAVGLANDSKVKRNQFMLGTEYYIVPKHLKGFVEW
                     QATRAKHYTNDVKTAKSRNYTTVVGLRAYW"
     gene            1562..2740
                     /gene="lpxB"
                     /locus_tag="PXA33_002322"
     CDS             1562..2740
                     /gene="lpxB"
                     /locus_tag="PXA33_002322"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HDL5121620.1"
                     /translation="MKQQFPVIGLVAGEVSGDILGAGLIKALKVHYPNARFIGVAGSR
                     MIEAGCQSLFDMEELSVMGLAEVVKHLPRLLKRRKQVIDEMLKLKPDVFIGIDAPDFN
                     LTVEEKLKASGIKTIHYVSPSVWAWRQKRVFKIARAANLVLAFLPFEKAFYDKFNVPC
                     RFIGHTMADAIPLEPNRTEACLALNIDESKRYMAILVGSRGSEIQFLAEPFLKAAQLL
                     KARFPDLQFLVPMVSQKRMEQFNAIKQQVAPELELNIIQGKARQAMIASECTLLASGT
                     AALEAMLCKSPMVVGYRMKPLTYWLAKKLVKTDYISLPNLLAQAPLVPELIQEACNPE
                     NLAQELSLFLADDEKNQQHKALLKQQFMQLHQSIQCNADEQAAQAVIDLLNGSTSGQI
                     "
     gene            2767..3819
                     /gene="sohB"
                     /locus_tag="PXA33_002323"
     CDS             2767..3819
                     /gene="sohB"
                     /locus_tag="PXA33_002323"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HDL5121621.1"
                     /translation="MWKEVLLNYGIFLLELLTVFGIIAVVVMLILESKKQNENGSISI
                     TNLSEKYKEQQKSLSGFFLSEEELKHQEKQEKKVAKEKAKAEKKRLKAGEECVESPSR
                     LFVLNFNGDMMAHEVNSLRREIDAVISLANPEKDEVLLKLESPGGVVHGYGLAASQLQ
                     RLRDRNIYLTAAVDKVAASGGYMMACVANKIVSAPFAVIGSVGVVAQVPNIHRLLKKH
                     DIDVDVMTAGEYKRTVTLVGENTEKGKQKFQQELEETHSLFKQFVARHRPQLDIEQVA
                     TGEHWFGQQAIELNLVDEIATSDDLLLNAIKEKEVIELKYKEKKKLAQKLGSQFEQSA
                     ENLLAKILHKSRSTLM"
BASE COUNT         1174 a          681 c          855 g         1239 t
ORIGIN      
        1 gactgtcaga ttaggtttga tcttctacat ataataaccc ctcatcactt tttgcagttt
       61 aacctctttt gcaacagata atttaaattt aggcgttttg gctgctcagt tattgccatt
      121 atgattatat tagttcacac taaaatttta gtgttgtttc ttaaagctga agatgctaat
      181 aggtgagtaa gcggttttta ccactttgaa gataaatgca atagcttctt gtttgaagga
      241 ttagttgtat ttaatcataa aaaatctgca ccttaatcag ttggttagcc caacttttag
      301 gtgcagatca ttctagtacg cttttcagtt ttattaccaa tatgctcgta agcctacaac
      361 agtcgtataa ttgcgagatt ttgcggtttt tacatcatta gtatagtgtt tagcacgagt
      421 tgcttgccat tcaacaaagc cttttaagtg ttttggaaca atgtaatatt ccgttcccaa
      481 cataaattgg tttcttttaa ctttgctatc atttgccaag cctacagcat taagtttgtt
      541 ttctttataa gcatacattg tgtaagcatt ccattgatcg gttaaacgat ggagtaacac
      601 agtgcgaact tctttttcga tacgtttgtg accgatagca ttttgatctt ccgtttcacg
      661 gcgttctaaa tctaagccaa gggtagtttt tccgtaggta taagctgtac cgaaggcaag
      721 agcaatgcgt tcataattgc cgatagattg ctcagaacgc tcacgggttg cacctgttgc
      781 taattttaag ctttggttat catcaatttt atagttataa attgctgctg caccccaaat
      841 atctttacgt ttattgacta aatctaagcc attgtttcca cgttggctat ttccgccata
      901 gaatgctccg agttttaatc cttcgatgcc tttgtaaaca tattgaagcg tacgtcgtga
      961 tgaaccggta agtaagccat cacttaagct gagtgtattt ggtaagtcag tttgttttac
     1021 ttcatcggca atattagtca tattgccgta tgttaattca ccgtattgtt tatgcccgat
     1081 tcctgcatag agttgatgat cataaatatg atcgaagtta tgttgtgagc ttgaatcgcc
     1141 acgcatacgc cattccacac gacctaaagc atagaatccg tgacccactt gctgggttaa
     1201 acggaaacca aaacgagagc cgttgttatc cactgcgtga ttaacgtggt tgcgggtagc
     1261 tttaccattt accggtgagg ttttatctga ggtgctttcc cacttaatac gagcagaacc
     1321 aataaattca atttgtgtac cggtttcttt atgatcgtaa atcgtaatag ctagagctga
     1381 gtttgcgatt aaggctgatg ctaaaatcgc taatgttgtt tttttcataa aaatcactcc
     1441 atatgaatag ataaagggag gtagtgtatg tgaatattca ttgctagaca ataatgtttt
     1501 gttagtttat atgatgattg gttataatag agtcacttac agatcttcaa taatatttaa
     1561 tatgaaacaa caattccccg ttattggctt agttgctggt gaggtctctg gcgatattct
     1621 tggtgccggg ttaattaagg cgttaaaagt acattacccg aatgctcgat ttatcggtgt
     1681 tgcaggctct cgaatgattg aggctggctg ccagtctttg tttgatatgg aagagctttc
     1741 tgtaatgggg ctagcagagg tggttaaaca tttacctcgt ttacttaagc gtcgtaaaca
     1801 ggttattgat gagatgctta aattgaaacc agatgtattt atcggtattg atgcaccgga
     1861 ttttaattta accgttgaag aaaaactcaa agcgagtggg attaaaacaa tccattatgt
     1921 tagcccttcg gtgtgggctt ggcgacaaaa gcgggtgttt aaaattgcca gagcagctaa
     1981 tttagtgttg gcatttctgc catttgagaa agcgttctat gataaattca atgtgccttg
     2041 tcgttttatc gggcatacga tggcagatgc gatcccactc gaaccaaatc gtacagaggc
     2101 atgtttagcg ttaaatattg atgaatccaa gcgatatatg gcaattttgg tgggaagccg
     2161 tggcagtgaa attcagtttt tggctgagcc ttttttgaag gctgctcagt tgttaaaagc
     2221 ccgttttccg gatttacagt ttttagtgcc aatggtcagc caaaagcgaa tggagcaatt
     2281 taatgcaatt aaacagcaag tagctcccga gttggaactg aatattattc aaggcaaggc
     2341 tcgtcaggct atgattgcgt cagaatgtac tcttttggca tcgggaacag cagcactaga
     2401 ggcaatgttg tgcaaatctc cgatggtagt tggttataga atgaaaccgc tgacttattg
     2461 gttagcaaaa aaattagtga aaaccgatta tatctcctta ccgaatttat tggctcaagc
     2521 cccattggta ccagaattga tccaagaagc atgcaacccg gaaaatttag ctcaagagtt
     2581 atctctattt ttagctgatg atgagaaaaa ccaacaacat aaagcattgc taaaacagca
     2641 gtttatgcag ctacatcaat caattcaatg taatgctgat gagcaagctg cccaagcggt
     2701 tatcgatctc ctaaacggct cgacaagcgg tcaaatttag ttagtttttt gtaaaaagga
     2761 aaatttatgt ggaaagaagt attacttaat tatggtattt ttttattaga gttattaact
     2821 gtatttggca ttattgcagt ggtggtgatg ctgattttgg aatcgaaaaa gcagaatgaa
     2881 aacggctcaa tttcaattac caatttatct gagaaatata aagagcagca aaaatctctt
     2941 tccggctttt ttctaagtga ggaagaatta aaacatcagg aaaagcagga gaaaaaagta
     3001 gcaaaagaga aagctaaagc agaaaaaaaa cgcttaaaag caggcgaaga atgtgtagaa
     3061 agcccttccc gtttatttgt attgaatttt aatggcgata tgatggctca cgaagtaaat
     3121 tccctacgcc gtgaaattga tgcggtaatt agcctcgcga atccggaaaa agatgaagtt
     3181 ttacttaaac ttgaaagtcc gggcggagtg gtgcacggtt atggattggc tgcttcgcag
     3241 ttacaacgtt tgcgtgatcg caatatttat ttaaccgcag cggtagataa agtagctgcc
     3301 agcggtggtt atatgatggc gtgtgtggcg aataaaattg tctctgcccc atttgcggtg
     3361 attggctcag tgggggtggt ggctcaggtg ccgaatatcc accgcttact gaaaaaacac
     3421 gatattgatg tggatgtgat gactgcaggt gaatataaac gcacggtgac gctggtggga
     3481 gaaaataccg aaaaagggaa acagaaattc cagcaagaac tggaagaaac gcactctctg
     3541 tttaagcaat ttgtggctcg acaccgccca caactggata ttgagcaggt tgcaacaggc
     3601 gagcattggt ttggtcagca agcgattgag ctaaatttgg ttgatgagat tgcaaccagt
     3661 gatgatctgc tcctcaatgc aattaaggaa aaagaagtga tcgagctaaa atataaagag
     3721 aagaaaaaac ttgctcagaa attgggttct caattcgagc aatcagcaga gaacctgctc
     3781 gctaagattt tacataaaag tcgttcaacg ctgatgtagc gtatatatta ttaaaggact
     3841 atcgtttata tagcccctta ttttatcagt tagtaataga atgaagtatt tgttcactat
     3901 ttaaatgacc tggaatactt ttctttaaat tgagtgaaga atctaacaa
//
LOCUS       DANLRK010000195         3898 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.196, whole genome shotgun sequence.
ACCESSION   DANLRK010000195 DANLRK010000000
VERSION     DANLRK010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3898
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.196"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(126..2690)
                     /locus_tag="PXA33_002324"
     CDS             complement(126..2690)
                     /locus_tag="PXA33_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HDL5121622.1"
                     /translation="MRIAKIIFSALLSLLVLGGVIGGLLYMHFKSDLPDVATLKNVEL
                     QQPMQIFTLDGKLIGEVGEERRIPVKLDDIPPMLVKAILATEDARFYEHKGIDPKGIM
                     RAIWRTSQGDTQGASTITQQLAKNFFLTPERSLERKAKEAILALEIEQVLSKNEILEL
                     YLNKIYLGYRSHGVAAAAKTYFNKSLKDLTLSEIAIIAGLPKAPSTMNPLYSVKRAED
                     RRNVVLGRMLETGDITKEEYEKAKAEPVVANYYGATLEFRADYVTEMVRQEIVKRYGE
                     NIAYNKGLKVYATVLSADQKAAQDALRENLIDYDRRHGWRGAEKLWTGSSAWDDEKII
                     DHLSKLPKSEPFTPAVVMQADKSKYTILLANGDISVLKRTNASFAPKGLKAGEQIWVR
                     QNKNREWALGQIPEVNSALVSINSENGAIESIVGGFSFEQSRFNRATQSLVQVGSAIK
                     PFIYTAAMNKGLSLSTTISDSPIVIKKKGQKEWRPKNADGVYGGPLRTRVALGKSKNM
                     VAIRILQMAGIDYVADYLQRFGFNRNQYVATESLALGAASFTPLEMARAYAVFNNGGY
                     LIEPYIIDRIIDAQGYELYQANPVVACNSCDSPVVYPEPKYFESVKIHDEAQVGVQTI
                     ANSSNNDDDDVVEDVGDSEPELQANNSEKNAPSLMAESSHHIKGLRYAPHVISDELAF
                     LMRSALATAITGEPEYGWRGTSYKMLNTIKRADVGGKTGTTNNAKATWYAGFGANIST
                     VVYVGFDDNKRVLGRGASGSSTALPAWTNYMKVALSDKPVEKSTLPKNIIEVNIDPNS
                     GFLGNGRKEYFIKGTEPTKRYIVERAYTEKAPQKTESAPLRLGLPPPGVLKSGELF"
     gene            2814..3512
                     /locus_tag="PXA33_002325"
     CDS             2814..3512
                     /locus_tag="PXA33_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComA"
                     /protein_id="HDL5121623.1"
                     /translation="MKLLKAHSKTPSNRIILGLSEDEQRYCLVKNKAEKFTVFWQAKP
                     ASIEELIGNLTPQEQAMMTLVRPIPYQYIWRKTVFMANTLNEAQLHRKVIHILKNEQP
                     LALDLLNIDYQRFPSSNDNLDKVVIYAVRKSYTENLSQFNSILDCELHCYARAIIHLT
                     QSQNNQPAPCFSFKQKYFQFTETELLILQTEPEDCIHLSDLKIDHLEIECEQEKQLYC
                     LALGASLWNGKVLI"
     gene            3488..>3898
                     /locus_tag="PXA33_002326"
     CDS             3488..>3898
                     /locus_tag="PXA33_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121624.1"
                     /translation="MEWQGIDLSANRKNNWFAGSDKGIKNVIYTLLLSALISLALIFL
                     YLQKNQEIQPLAQQLELVKTDILNIENKIISLNSNHTNTLPFAEPKKIAQLLAIIDNL
                     PLKNGGIETIQIYLETDLYLKIIGNLDNQSDFQSL"
BASE COUNT         1208 a          852 c          639 g         1199 t
ORIGIN      
        1 tcatttattg actaaggaat atccaaatat aactcacaaa tcgcccaaaa taaaattggc
       61 acttattatc gcaagaaatc cccctatttt tcaactttta agagcattat ccttgtaata
      121 aattattaga ataattcacc gctttttagc acccctggtg gaggtaatcc taaacgtaac
      181 ggcgcagatt ctgttttttg aggcgctttt tcagtataag ctctctctac aatataacgt
      241 tttgtaggct cagtaccctt aataaaatac tctttacgtc catttcctaa gaaaccacta
      301 ttagggtcaa tattaacctc aataatattt tttggaaggg tacttttttc gacaggctta
      361 tctgacaatg caactttcat ataatttgtc caagctggta aagcagtact agaacctgaa
      421 gcacctctac ctaatactct tttattatca tcaaagccaa cgtacacaac agtagaaata
      481 ttagcaccaa atcccgcata ccaagttgcc ttggcattat tggttgtacc agtcttcccg
      541 cccacatccg ctcgttttat cgtattgagc attttatagc ttgttcctcg ccagccatat
      601 tcaggctcac cagtaattgc tgtagctaaa gcactacgca ttaaaaaagc gagttcatca
      661 ctaataacat gaggagcgta acgtaaccct ttgatatgat gagaagattc tgccattaag
      721 ctcggcgcat ttttctcgct attgttcgct tgtaattcag gttctgaatc gccaacatct
      781 tccacaacat cgtcgtcatc attattcgag gaattcgcta tagtttgtac accgacttga
      841 gcttcatcat gaattttgac tgattcaaaa tatttaggct cagggtaaac aactgggcta
      901 tcgcaactat tacaagctac tacaggattt gcttgatata actcgtagcc ttgggcatca
      961 ataatacggt caatgatata tggttcaatc aaataacctc cattattaaa tactgcataa
     1021 gctcgagcca tttctaatgg cgtaaaagat gcagcaccca atgcaagaga ttctgttgca
     1081 acatactgat ttcggttaaa gccaaaacgt tgtaagtagt ctgcaacata atcaatccct
     1141 gccatttgta atatacgaat agccaccata tttttcgatt tacctaatgc aacacgagtt
     1201 ctcaacgggc cgccataaac cccatcggca tttttaggtc gccactcttt ttgccctttc
     1261 tttttaatga caataggaga atcgctaatt gttgttgaaa gacttagccc tttattcatt
     1321 gccgctgtgt aaataaacgg tttaatcgca gagcctacct gaactaatga ctgagtcgca
     1381 cgattgaaac ggctttgttc aaagctaaag cctccaacga tagattcaat ggcaccgttt
     1441 tcactattaa ttgacactag ggctgaattt acctccggaa tttgtcctaa agcccactcc
     1501 ctatttttat tttgacgtac ccaaatttgt tcacctgctt ttaacccttt aggtgcaaaa
     1561 gaagcatttg tacgttttaa aacagagata tcaccatttg ccaataaaat tgtatatttt
     1621 gatttatctg cttgcattac aacagctggt gtaaacggct ctgattttgg cagcttactt
     1681 aaatgatcga taattttctc atcgtcccaa gcagaactac ctgtccataa tttttcagcg
     1741 cctcgccaac catgacggcg gtcataatca atcaaatttt cacgtaacgc atcttgtgct
     1801 gctttttggt ctgctgaaag taccgtggcg tacactttta agcctttgtt ataagcaata
     1861 ttttctccat agcgtttcac aatttcttga cgaaccattt ctgtcacata atccgctcga
     1921 aattctaatg tcgcaccata gtagttagct actactggct ctgctttagc tttctcatat
     1981 tcctcttttg taatatctcc ggtttctagc attctcccaa gcactacatt acgacggtct
     2041 tccgcccgtt ttactgaata aagtggattc atcgttgaag gcgcttttgg aagccctgca
     2101 ataattgcta tttcagaaag ggttaaatct ttcaaagatt tattaaaata agttttagcc
     2161 gcagcggcaa caccgtgaga acgataccct aggtaaattt tgtttaaata cagctccaga
     2221 atctcatttt tactgagaac ctgttcaatt tctaaagcta aaattgcctc tttggcctta
     2281 cgctctaaac tacgttcagg tgttaagaaa aagtttttcg ccagctgctg ggtgattgta
     2341 cttgcgcctt gagtatctcc ctggctagtg cgccatattg cacgcataat acctttagga
     2401 tcgatccctt tatgctcgta aaaacgtgca tcttcggtag caagaatcgc ttttactaac
     2461 atcggtggaa tatcatcaag cttgaccgga atacggcgtt cttctcctac ttcaccaatt
     2521 aatttaccat ctaaggtaaa aatctgcata ggctgctgta actctacatt tttcagcgtt
     2581 gccacatcag gcaaatcaga tttgaaatgc atgtacagta aaccaccaat taccccacct
     2641 aaaaccaaga gtgaaagtaa agcactaaat attatttttg cgatccgcat cgaaaaaatc
     2701 tcttttggtt aatgacgaaa cataaattta aagccaatat taggtcacta agtataagct
     2761 aaaaacgctt acttcgcata actaattgca aaactttcct tactatactt actatgaaat
     2821 tacttaaagc acattcaaaa acaccttcaa accgtatcat cttaggctta agtgaagatg
     2881 aacaacgtta ctgcttagtc aaaaataaag cagaaaaatt caccgttttt tggcaagcca
     2941 agcctgcgag catcgaagag cttatcggta atctcacccc acaagaacaa gcaatgatga
     3001 cgctggttcg cccgatccct taccaataca tttggcgaaa aaccgttttt atggctaaca
     3061 ccctcaatga agctcaatta caccgtaagg taattcacat tttaaaaaat gagcagccac
     3121 ttgcattgga tctactcaat attgactatc agcgatttcc aagcagcaac gataatctgg
     3181 ataaagtggt tatctacgcc gtgcgaaaaa gttataccga gaatttgagc caatttaaca
     3241 gcatcttaga ttgtgagctg cattgctatg cacgggctat aattcattta acacaatccc
     3301 aaaataatca gcctgcgccc tgtttttcat ttaaacagaa gtacttccaa tttaccgaga
     3361 cggaattact gatattgcaa acagaacctg aggattgtat tcatctatct gatctcaaaa
     3421 ttgaccatct tgaaatcgag tgtgagcaag agaaacagct atattgcctc gccttaggag
     3481 caagtttatg gaatggcaag gtattgattt aagtgctaat cgaaaaaata actggtttgc
     3541 cggcagtgat aaaggcatca aaaacgtgat ttatacactt ttattatctg ccctcattag
     3601 cttggctctg atttttctct atttgcaaaa aaatcaagaa attcaaccgc ttgcacagca
     3661 attagagctg gtaaaaaccg atatattaaa tattgaaaat aaaattatca gcttgaatag
     3721 taatcataca aacaccttgc cctttgccga gcctaaaaaa atagcacaac tactggcaat
     3781 aatagataac ttaccgctca aaaatggggg aattgaaacg atacaaattt atcttgaaac
     3841 cgatctctat ttaaaaataa tcggtaattt agataatcaa tctgattttc aaagtcta
//
LOCUS       DANLRK010000196         3845 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.197, whole genome shotgun sequence.
ACCESSION   DANLRK010000196 DANLRK010000000
VERSION     DANLRK010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3845
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.197"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            254..586
                     /locus_tag="PXA33_002327"
     CDS             254..586
                     /locus_tag="PXA33_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF413 domain-containing protein"
                     /protein_id="HDL5121625.1"
                     /translation="MADSFSVTRRFFDDKNYPRGFSRHGDYTIRESQTLEQFGQACLA
                     LESGERKPATQEEKRFVAVMKGEEVAETDIEKAWLKYRTLTSKTKRIYTLSGNAGSDA
                     GDDFSAAE"
     gene            651..1742
                     /gene="trmA"
                     /locus_tag="PXA33_002328"
     CDS             651..1742
                     /gene="trmA"
                     /locus_tag="PXA33_002328"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HDL5121626.1"
                     /translation="MTLPIEQYSDLLAKKTENLTALLKPFNPPAIEVFASKPSHFRMR
                     AEFRVWHEEGELYHIMFNPETKARYRVDSFPIASELINHMMTALLAEIKGNELLTRKL
                     FQIDYLSTLSGEIAVSMLYHKSLNEEWVEQANALKSRLEQQGFKVQIIGRANKQKIAL
                     DCDYVEEILPVHGKNYVYRQVENSFTQPNAEMNIKMLEWARNCTKNSEGDLLELYCGN
                     GNFSIALAENFRKVLATEISKSSVQSAQYNIEKNGIVNLQIIRMSAEEFTQAMNGVRA
                     FYRLKGIDLNAYECNTIFVDPPRAGLDQATLNMVQAYERILYISCNPETLAENLQQLS
                     QTHRIEKAALFDQFPYTHHIESGVWLIRK"
     gene            1834..1923
                     /locus_tag="PXA33_002329"
     tRNA            1834..1923
                     /locus_tag="PXA33_002329"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1868..1870,aa:Ser,seq:tga)
     gene            2079..2450
                     /locus_tag="PXA33_002330"
     CDS             2079..2450
                     /locus_tag="PXA33_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HDL5121627.1"
                     /translation="MKYLYIICGFICIGLGIIGAVLPGLPTTPFLLLALFCFTKGSER
                     IRQWFIQTKLYKKYLQEYDEKRAMTMKQKITILAISFPFTLFAFFALPHIGGKIALVV
                     LVIIQYWYFFCKMKTLEPENA"
     gene            complement(2447..3358)
                     /gene="xerC"
                     /locus_tag="PXA33_002331"
     CDS             complement(2447..3358)
                     /gene="xerC"
                     /locus_tag="PXA33_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HDL5121628.1"
                     /translation="MTTSNPLFQQTRPYWDFLRIEKQVSPHTLSNYQRQLMAVSELFS
                     QAGIENWEEVESSSVRWMLSQSHKQGLSAKSIGLRLVALRQWFVYLIKIGRMSANPTL
                     GVKSPKVGKHLPKNIDAEQVGQLLAIESDEPSDIRDIAMMELMYSSGLRLSELQGLDL
                     GDMDLSAREVRVQGKGSKERIVPIGSQALKALNRWLEMRLQFKPQDNAVFLNKRGGRL
                     SHRSIQLAMKKWGERQGLESHLHPHKLRHSFATHMLEASGDLRAVQELLGHSSLSTTQ
                     IYTHLDFQHLAKIYDSAHPRARRKQED"
     gene            complement(3425..3715)
                     /locus_tag="PXA33_002332"
     CDS             complement(3425..3715)
                     /locus_tag="PXA33_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5377 domain-containing protein"
                     /protein_id="HDL5121629.1"
                     /translation="MSVKTEVLFSNHWNVRVSDPGFEGSPSHYFETVYLTLEAFINGE
                     NVHYEFTRKVEQEVKTHRSFTDVNELFEFLGEYLSPVALGNVGVRIGQLGLA"
BASE COUNT         1149 a          841 c          737 g         1118 t
ORIGIN      
        1 cacagtatat ctccaaatat taaagttaat caaaactcaa tacatttagg taatatacct
       61 taacaaatat aaaaaatata cctcagactg cattgtctat tttaaaagga tcaatatttc
      121 tccttaaaga gttttttgca aaaaatttgc caaatttgac cgctttttta cattaaggca
      181 ttgatttatt cgctgttttt gtttaatgta tgccgatttt attttcccta atttcaaaca
      241 acgaggcatt attatggcag acagtttcag cgttacgcgc cgcttttttg acgataaaaa
      301 ttatccacgt ggtttctctc gccacggcga ttacaccatt cgtgaatcac aaactttaga
      361 acaattcggt caagcttgct tggctttaga aagtggtgaa cgtaagccgg caactcaaga
      421 agaaaaacgt ttcgttgcgg taatgaaagg cgaagaggta gcagaaactg acattgaaaa
      481 agcgtggtta aaataccgca cattaactag taaaacaaaa cgcatctaca ccctttcagg
      541 caatgcaggt agcgatgccg gcgatgactt cagtgctgct gaataatttc taatttaaaa
      601 ccacgaagta attcgtggtt tgttttttca tttgctattt gtggtaaaaa atgactcttc
      661 ctatcgaaca atattccgat cttcttgcaa aaaaaaccga aaatttgacc gctttactca
      721 agccgtttaa cccgcctgcg attgaggtat ttgcctcaaa gccaagccat ttccgtatgc
      781 gggcagaatt tcgagtgtgg cacgaggaag gcgagctgta ccacattatg ttcaaccctg
      841 aaaccaaagc tcgttatcgt gtggatagct tccctattgc cagtgagctg attaaccata
      901 tgatgaccgc attattggcg gaaattaaag gcaatgaact actgactcgc aagctgttcc
      961 agatcgatta tttaagcacc ttaagcggtg aaattgcagt ctcaatgctt taccacaaaa
     1021 gcctgaatga agaatgggtt gaacaggcaa acgccttaaa aagccgttta gagcaacaag
     1081 gtttcaaagt gcaaattatc gggcgtgcca ataaacaaaa aatcgcactg gattgcgatt
     1141 atgtggaaga aattctaccg gtacacggca aaaattatgt gtatcgccaa gtggaaaaca
     1201 gctttaccca gccgaatgcg gaaatgaata ttaaaatgct ggaatgggcg agaaattgca
     1261 caaaaaatag tgaaggcgat ttgcttgagc tttactgcgg caacggcaat ttttcgattg
     1321 ccttggcgga aaacttccgc aaagtgctgg caaccgagat ctcaaaatct tcagtgcaat
     1381 cggctcaata caatattgaa aaaaatggca ttgttaacct acaaattatc cgaatgtcgg
     1441 cagaagaatt tacccaagcg atgaacggtg tgcgagcctt ttatcgctta aaagggattg
     1501 atttaaacgc ctacgaatgc aataccattt ttgttgatcc acctcgtgca gggttagatc
     1561 aagctacatt aaatatggtg caagcgtatg agcggattct ctatatctct tgcaatccgg
     1621 aaacccttgc cgagaattta caacaattaa gccaaaccca tcggattgaa aaagccgctc
     1681 tttttgacca attcccttac acccaccata ttgaaagtgg cgtttggtta attagaaaat
     1741 aaattgtgct gaaaaaaaga acgaataagc agcttttaag atttattact tgacgatgtt
     1801 cgcaataatc tctattattg caacgttttc aacggaggaa tggcagagcg gttgaatgca
     1861 ccggtcttga aaaccggcga gggttaacgc cctccgtgag ttcgaatctc acttcctccg
     1921 ccagtcttta aacctgttga gcaatcagca ggttttttta tttcatttgc aagaaaatca
     1981 gataaaacca ccgcttgaat ccatcgctat aaactggttg aaatcagcct aaagttgctt
     2041 taggataatt gcgattcatt atcaataact taattattat gaaatacctt tatattatct
     2101 gtggttttat ttgtattggt ttaggcatca tcggggcagt attaccgggc ttgccgacca
     2161 caccgttctt acttttggct ctgttctgct ttactaaagg ctcggagaga atccgccagt
     2221 ggtttattca aaccaaatta tacaaaaaat atctgcaaga atatgatgag aaacgggcaa
     2281 tgacgatgaa gcaaaaaatc acgattttag cgatttcgtt tccgtttaca ctatttgctt
     2341 tctttgcact gccccatatc ggtgggaaaa ttgcgttagt agtgttagtg ataattcaat
     2401 attggtattt cttctgcaaa atgaaaaccc ttgaaccgga gaatgcctaa tcttcctgct
     2461 tccgccttgc tcttgggtgg gcagagtcat aaattttagc taagtgttgg aaatccagat
     2521 gcgtataaat ttgagtggtg gacaggctac tatgacccaa taattcctga accgctcgca
     2581 aatcaccgct tgcctctaac atatgagtcg caaaagagtg acgtaattta tgtggatgca
     2641 gatggctttc cagcccttgt cgctcacccc atttcttcat tgccaactga atggaacgat
     2701 gagataaccg cccacctcgc ttattcagaa acaccgcatt atcttgcggc ttaaattgca
     2761 aacgcatttc taaccagcga tttaacgctt ttaacgcttg cgagccaatc ggcacaattc
     2821 gctctttgct acccttacct tgcacccgta cttctcgtgc cgataaatcc atatcgccta
     2881 aatccagccc ttgtaattcc gataaacgca agccggaact atacatcagc tccatcatcg
     2941 caatatctcg aatatcgctc ggctcatcac tctcaatcgc cagcaactgc ccgacctgct
     3001 ccgcatcaat atttttcggc aaatgcttac ctactttcgg cgatttcacc cctaaagtcg
     3061 ggtttgccga cattcgcccg attttaatca aatagacaaa ccattgccgt aacgccacca
     3121 agcgtaagcc gatacttttc gcactcaacc cttgcttatg gctttggctt aacatccaac
     3181 gcaccgagct actttcgacc tcttcccaat tttcaatgcc tgcttgcgaa aaaagctcgc
     3241 tcaccgccat taattggcgt tgataattgc ttaaggtatg cgggctaact tgcttttcaa
     3301 tgcgtaagaa atcccaatag gggcgggttt gttggaaaag agggttggaa gtggtcataa
     3361 acgtaataac aagcggttaa tttttaggaa aatgttgcaa gggcaaaaga tttgcccttt
     3421 gagattaggc tagccccaac tgaccgatac gcacgccaac attaccaagt gcaacagggc
     3481 ttaaatattc gccgagaaat tcaaacaatt cattcacatc agtaaaactg cgatgagttt
     3541 tcacttcttg ctccacttta cgagtaaatt cataatgtac attttcgccg ttaataaagg
     3601 cttccagcgt taaatatacg gtttcaaaat agtggcttgg cgagccttca aagcccggat
     3661 cactcactcg aacattccaa tgattactaa ataaaacttc ggtttttact gacattgtgt
     3721 tttccttctt cctataagta cgtacattat tttaatcaag atagattgaa tttgccactt
     3781 ttcctagtca gaatcgaggc aaatcttatt tggtttgtcc tcttatatct ttccctcaaa
     3841 atatg
//
LOCUS       DANLRK010000197         3815 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.198, whole genome shotgun sequence.
ACCESSION   DANLRK010000197 DANLRK010000000
VERSION     DANLRK010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3815
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.198"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            <1..1606
                     /gene="pckA"
                     /locus_tag="PXA33_002333"
     CDS             <1..1606
                     /gene="pckA"
                     /locus_tag="PXA33_002333"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HDL5121630.1"
                     /translation="LNRIEQELADLGITNVKEIVRNPSYEQLFEEEMKSDLKGYEKGV
                     LTNLGAVAVDTGIFTGRSPKDKYIVLDETTKDTVWWTSDAAKNDNKPMTQSTWQSLKT
                     LVTDELSNKRLFIIDAFCGASPEHRINVRIITQVAWQAHFVKNMFIRPTEEELKDFKP
                     DFVVMNGSQVTNPNWKEQGLNSENFVAFNITEGVQIIGGTWYGGEMKKGMFSMMNYFL
                     PLKGVASMHCSANVGEEGDVAVFFGLSGTGKTTLSTDPKRKLIGDDEHGWDENGVFNY
                     EGGCYAKTINLSIENEPDIYRAIRRDALLENVVVREDGTIDFDDKSKTENTRVSYPIY
                     HIDNIAVPSKAGHAKKVIFLTADAFGVLPPVSKLTPEQTKYYFLSGFTAKLAGTERGI
                     TEPTPTFSACFGAAFLSLHPTQYAEVLVKRMEAAGAEAYLVNTGWNGTGKRISIKDTR
                     GIIDAILDGSIEKAEMSTLPIFNLAIPTALPGVETHILDPRNTYEDKAQWQAKAEDLA
                     GRFVKNFEKYATNEEGKALIAAGPKL"
     gene            1699..2700
                     /gene="bioB"
                     /locus_tag="PXA33_002334"
     CDS             1699..2700
                     /gene="bioB"
                     /locus_tag="PXA33_002334"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HDL5121631.1"
                     /translation="MTTFQLRLKAKDELTPHPTAQYWRKCQVEGLFDLPFMDLVFRAA
                     QVHRENFDANAIQLSTLLSIKTGGCPEDCEYCPQSARYDTGIEKQTMLDVETIVEKAK
                     IAKERGASRFCMGAAWRGPRPKDIKVVSEIINAVKSLGLETCGTFGMLEEGMAEELKQ
                     AGLDYYNHNLDTDPERYNKIIHTRNHEDRMDTLGKVRNAGLKVCCGGIVGMNETRPER
                     AGLIASLANLDPQPESVPINQLIKVEGTPLTNAEDLDWTEFVRTIAVARITMPQSFVR
                     LSAGRTTMPEAMQALCFMAGANSIFYGDKLLTTENPEEDHDRQLMDKLDLYPLQYIA"
     gene            2860..2957
                     /gene="ffs"
                     /locus_tag="PXA33_002335"
     ncRNA           2860..2957
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PXA33_002335"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            2992..3699
                     /gene="gloB"
                     /locus_tag="PXA33_002336"
     CDS             2992..3699
                     /gene="gloB"
                     /locus_tag="PXA33_002336"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HDL5121632.1"
                     /translation="MLQITPIPALKDNYIWAIRNGQDVIIIDPSEHQPALNFIAKNAL
                     NLTAILITHNHHDHTDGVSGIVEQYPNIPVYGPQEVAEFANVIVSPEQHISLFGYDVR
                     IIESGGHTAGHISYLFGYDYLFCGDALFSGGCGRVFTGDYQAQFDALQRFKNLPNSVK
                     AYPAHEYTKSNLKFAEAVLPPSCVLSEYQEQVDLLRSQNKPTLPTTIGLEMQVNPFMQ
                     AVDLAEFIALRQQKDNF"
BASE COUNT         1181 a          741 c          835 g         1058 t
ORIGIN      
        1 gttaaatcgt attgaacaag aacttgcaga cttaggcatt acaaatgtaa aagaaatcgt
       61 gcgtaaccct agctatgagc aactgtttga agaagagatg aaatcggatc ttaaaggtta
      121 tgaaaaaggc gtcctaacaa atttaggcgc agtagcggta gataccggca ttttcacggg
      181 gcgttcgccg aaagataaat atatcgtatt agatgaaacg acaaaagata ccgtctggtg
      241 gacatctgat gcagctaaaa atgataataa accgatgact caatctactt ggcaaagttt
      301 aaaaacctta gtgaccgatg agttatctaa taagcgttta tttattattg atgctttctg
      361 tggtgcaagc cctgagcatc gcatcaatgt gcgtattatt acccaagttg cgtggcaagc
      421 tcactttgtg aaaaatatgt ttattcgtcc aacagaagaa gaattaaaag atttcaaacc
      481 ggattttgtg gtaatgaacg gttcccaagt aactaacccg aactggaaag agcaaggctt
      541 aaattcagaa aactttgtgg catttaatat cacagaaggt gttcagatca ttggcggtac
      601 ttggtacggt ggtgagatga aaaaaggtat gttctctatg atgaactact tcttaccatt
      661 aaaaggtgtc gcttcaatgc actgttcagc caatgtgggc gaagaaggtg atgtggcggt
      721 tttctttggt ttatcaggaa ccggtaaaac gacactatca accgatccga aacgcaaatt
      781 gattggtgac gatgaacacg gttgggacga aaacggcgta tttaactatg aaggtggttg
      841 ctatgcgaaa accatcaacc tttctattga aaacgagccg gatatttatc gtgcaatccg
      901 ccgtgatgcg ttattagaaa acgtggttgt gcgtgaagat ggcactattg attttgatga
      961 taaatcaaaa acagaaaata cccgtgtgag ctatccgatt tatcatattg ataatattgc
     1021 cgtgccgtca aaagcaggac acgctaaaaa agtgattttc ttaacggcag atgcattcgg
     1081 cgtattgcca ccggtgtcta aattaacgcc tgaacaaact aaatactact tcttatcagg
     1141 ctttaccgca aaattagcag gtacagagcg tggtattact gagccaaccc caactttctc
     1201 tgcttgtttc ggtgcggcgt tcttatcact ccacccgaca caatatgctg aagtgttagt
     1261 gaaacgtatg gaagcagccg gtgcggaagc ctatttagtg aataccggtt ggaacggcac
     1321 aggtaaacgt atttcgatta aagatacacg gggtattatt gatgccatct tagacggctc
     1381 aattgagaaa gcggaaatga gtacattacc aatcttcaat ttagcaattc caactgcgtt
     1441 accgggtgta gaaacccaca ttttagaccc tcgtaacact tatgaagaca aagcacaatg
     1501 gcaagctaaa gcagaagatt tagctggcag atttgtgaaa aactttgaaa aatatgcgac
     1561 caacgaggaa ggcaaagcat taattgctgc tggtccgaag ttataagttg taattataag
     1621 ttataatcgc taaaaaatta gtttaggact aaaccttgaa tgaggtttag tccttttgta
     1681 taaaggagaa gtacaataat gacaacattc caattaaggc tgaaagccaa agatgagctt
     1741 accccacacc caacagcaca atattggcga aaatgccaag tggaaggact atttgatctg
     1801 ccgtttatgg atttagtgtt tagagcggca caagttcacc gtgaaaattt tgatgctaat
     1861 gcgatccagc tttcaacgtt actctcaatt aaaacaggag gctgtccgga agattgcgaa
     1921 tattgccccc aatctgcccg ctatgatacg ggaatagaaa agcaaacaat gcttgatgta
     1981 gagacgattg tagaaaaagc taaaattgcc aaagagcgtg gagcaagccg cttttgtatg
     2041 ggggctgcgt ggcgaggacc taggcctaaa gatattaaag tagtctcaga aatcatcaat
     2101 gcggtgaaat cattggggct agaaacctgt ggtactttcg gtatgcttga agagggaatg
     2161 gcagaagaat taaagcaagc agggctggat tactacaacc ataatttaga taccgaccct
     2221 gaacgctata acaaaattat ccatacccga aatcacgaag atcgaatgga cactttaggt
     2281 aaagttcgca atgccggctt gaaagtttgc tgtggcggta ttgtgggaat gaacgaaacc
     2341 cgccccgagc gagcaggcct aattgcaagc ctcgctaacc tcgatcctca acctgaaagc
     2401 gtaccgatta atcagttgat taaagtagaa ggtacaccgc ttaccaatgc cgaagattta
     2461 gactggacag aatttgttcg taccattgcg gtagcaagaa ttactatgcc tcaaagcttt
     2521 gtccgacttt ctgctggcag aactactatg ccggaggcaa tgcaagccct ctgttttatg
     2581 gcaggagcaa attctatttt ctatggcgat aaattactta ctacagaaaa tccggaagaa
     2641 gatcatgatc gccaattaat ggataaatta gatttatatc cgctacaata tatagcctga
     2701 gtgccaatga ttttcgcctg aatgaaaatt aaagaatcaa tcgttatttt acgcaaattt
     2761 ctaacataaa ataaccgctt gcaaaattcg ttcttgtctt atttttaaat ttatctataa
     2821 tgtgaggcct tacgcaagct tgcgtaattc gatggggcgt tattggttcc tcgcaatggt
     2881 gttcagctta cttgatcagg ttcggaagaa agcagtctgg gttgaaattt ccgtgtgccg
     2941 agggaggctg gtaatgcccc accctttttt cagttctatc aggagaacgt tatgctccag
     3001 atcaccccca ttccggcatt aaaagataat tacatttggg caatccgtaa cgggcaagat
     3061 gtgattatta ttgatccatc agaacatcag ccagcattaa actttattgc aaaaaatgcg
     3121 ttaaatttaa ccgctatctt gattacccat aatcaccacg accatactga tggggtgagt
     3181 ggtattgttg agcaatatcc gaatatccct gtttatggtc cgcaagaagt ggctgaattt
     3241 gccaatgtga ttgtctcgcc tgaacaacat atcagcctgt ttggttatga tgtaaggatt
     3301 attgaaagtg gtgggcatac cgcagggcat atcagttatt tatttggcta tgattatctg
     3361 ttttgtggcg atgcgttatt ctccggcggt tgcgggcggg ttttcaccgg tgattaccaa
     3421 gcccaatttg atgcgttaca acgctttaaa aatctgccta actcggttaa agcctacccg
     3481 gctcacgaat acaccaagag caatttgaaa tttgcagaag cggttctacc accaagctgt
     3541 gtattatcgg aatatcaaga acaggttgat cttttacgtt cgcaaaacaa accgactttg
     3601 ccaaccacta ttggcttgga aatgcaggtg aacccgttta tgcaagcggt cgatttagcc
     3661 gaatttattg cacttcgtca gcaaaaagac aatttttaac ctcatatttc taattaagca
     3721 ttaaagcctc tcactttaat gctttttggt taaataaaac ctttgcaaac atcgcttgtc
     3781 aattccactc tggcttatcc tatggtatag ttacg
//
LOCUS       DANLRK010000198         3798 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.199, whole genome shotgun sequence.
ACCESSION   DANLRK010000198 DANLRK010000000
VERSION     DANLRK010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3798
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.199"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(<1..329)
                     /locus_tag="PXA33_002337"
     CDS             complement(<1..329)
                     /locus_tag="PXA33_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121633.1"
                     /translation="MTLETFNQQKAKFHEQEKAVLAIQTELDKAKNILEALQNEKAEF
                     VARQKTNLANIGTLSADEYVEIKNKNSGLQARIEYYQALIVDLENKCYAEQEILFQQQ
                     NELKQIRR"
     gene            complement(339..545)
                     /locus_tag="PXA33_002338"
     CDS             complement(339..545)
                     /locus_tag="PXA33_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HDL5121634.1"
                     /translation="MENTPAKQRFMKIDEVSAVTTLSETQIYRKMKAGDFPQSISIGA
                     NSKVWLESEIQQWIQRKIEQHRNQ"
     gene            complement(1015..2229)
                     /locus_tag="PXA33_002339"
     CDS             complement(1015..2229)
                     /locus_tag="PXA33_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL5121635.1"
                     /translation="MTATKKPLSDTLLKNLKYSPNAKPIADSNGLFILAQKKAKVWIY
                     SYISPKTGKRSTKNRIGSYPQMSLAEARLKRDELNTLVLQGLDPFEHLAKMQREAKEK
                     QESIQSFAYKWADWKLSKGKCKPKTMEKCLQRLENHLFPRFEGYTLEDFTLTDTINRL
                     TDLDRIMPDTLHRIAGNLIEILDYAVLCGRINFNPISVIKKAFSSVSTTHQPAIMPEE
                     LAEFMQDLQKSNRTPQIKLLVEWQLLNILRPFEAVAVQWSDIDWETKTLNIPADRMKG
                     GKNPHSVPLTKQAIAILEEMKKYNGHHKHIFTHRTDRNKPANSQTVNNAIKRLSGGKY
                     KGLLVAHGLRSIASTYLHERFTTETLVIEACLSHTNKDTVMSAYHRKNWLDRRRGIMQ
                     EWADFVEQCKSI"
     gene            complement(2432..2507)
                     /locus_tag="PXA33_002340"
     tRNA            complement(2432..2507)
                     /locus_tag="PXA33_002340"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2472..2474),aa:Ile,seq:cat)
     gene            complement(2601..3338)
                     /gene="pgeF"
                     /locus_tag="PXA33_002341"
     CDS             complement(2601..3338)
                     /gene="pgeF"
                     /locus_tag="PXA33_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HDL5121636.1"
                     /translation="MDKIIPNWNVPKHIHALTTLRTGGVSKPPFDSFNLGDHVNDEPQ
                     DIAQNRALLVEKFQLPQAPLFLTQTHSTRVIELPYSGDNIKADAIYTNQPNQVCLVMT
                     ADCLPVLFASQDGSEIAAAHAGWRGLCDGILEATVEKFNCPPHEISAWLGPAIGPNAF
                     QVGSEVADQFCAFDPRAKEALIEDSTTSGKFLGNLYQIATQRLNKLGITAISGGEYCT
                     YSQPELFFSYRRDKQTGRMATLIWRTE"
     gene            complement(3370..3756)
                     /locus_tag="PXA33_002342"
     CDS             complement(3370..3756)
                     /locus_tag="PXA33_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015432850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDL5121637.1"
                     /translation="MTVIHTENAPAAIGPYVQAVDLGNMVLTSGQIPVNPETGEIPSD
                     IVQQTRQSLNNVKAIIEQAGLTVADIVKTTVFVKDLNDFAKVNAEYQAFFQENEHPNF
                     PARSCVEVARLPKDVGIEIEAIAVRR"
BASE COUNT          947 a          730 c          815 g         1306 t
ORIGIN      
        1 cgtctgattt gttttagttc attctgctgt tggaataata tttcttgctc tgcgtagcat
       61 ttattttcta aatcaacaat caacgcttga taatattcta tgcgtgcttg taaaccacta
      121 tttttatttt tgatttcaac atattcatct gctgaaagcg tgccgatatt ggctaaattc
      181 gttttttgcc gtgcgacaaa ttccgctttt tcgttttgca atgcctcaag aatatttttc
      241 gctttgtcta attcggtctg aatagctaaa acggcttttt cttgttcgtg gaatttagct
      301 ttttgttggt taaatgtttc aagtgtcata atttgccctt attggttgcg gtgttgttca
      361 attttgcgtt gaatccattg ctggatttca ctttctagcc atactttgct atttgcccca
      421 atactgatac tttggggaaa atcgccggct ttcattttgc gataaatctg cgtttctgaa
      481 agcgtagtta cggctgaaac ttcatctatt ttcataaatc gctgttttgc tggtgtgttc
      541 tccattttgt tttactccgt aaaaatttgc tttggcgtgc tattgcctgc taacgtgtga
      601 aagaataaat catcttggat tttttgctat ttgttagggc tgtgaaagga gattttacag
      661 attagagggg cttaattctg atttggaaaa cttgcaagcg gttatttttc tctgattttt
      721 tgcaagtttt cagcggttct ttcatcgttc tgtcatcgag tttttaaaag tggtgtttct
      781 gcctgcgtaa atttgcgttt tcttgcgtaa aattgcgtaa taaaaaacgg ctatatgaaa
      841 gtttttcacg ctatacgggg caatgtttgg gcttaatttt gcatttgcgt agaaatcgag
      901 actttaagtg tgcgggcgag gcgtgggata gacggcacgg gctgggagct gataggaaaa
      961 aatgaaaatt tttcatcaag caaaaagaaa ggcggattgc tccgcctttg tcatttaaat
     1021 gctcttgcat tgttctacaa aatccgccca ttcctgcatt attccacgtc tgcggtctag
     1081 ccagttttta cggtggtatg cactcattac ggtgtctttg ttcgtgtggg ataaacaagc
     1141 ctcaattact agcgtttcag tagtaaaacg ttcgtgtaaa taggtgctgg caatgcttct
     1201 taaaccgtga gccactaaaa gccctttata ttttccaccg cttaagcgtt ttattgcatt
     1261 attcacggtt tggctattag ccggtttgtt tcggtctgtt cggtgtgtaa aaatatgttt
     1321 atggtgtccg ttatatttct tcatttcctc taggattgcg atagcttgtt ttgttagcgg
     1381 tacagaatgc ggattttttc cgcctttcat tctatctgcc ggaatgttga gcgtcttagt
     1441 ttcccaatct atatcgctcc attgcaccgc tacggcttca aacggtctaa gaatattcaa
     1501 gagttgccat tcaactaata atttaatttg aggcgttcta tttgatttct gcaagtcttg
     1561 cataaattcg gctaactctt cgggcattat tgctggctgg tgtgtcgtgc ttacgctgga
     1621 aaacgccttt ttaatgacgc tgattgggtt aaaattaatt ctcccacaca ataccgcata
     1681 atcaagaatt tctattaaat tccctgctat tctgtgcaaa gtgtcgggca ttatccgatc
     1741 taagtccgtt aagcggttga ttgtatcggt taaggtaaaa tcttccagcg tatagccttc
     1801 aaaacgtggg aataaatgat tttctaaccg ctgtaagcat ttttccattg ttttaggctt
     1861 gcattttccc tttgatagct tccaatcagc ccatttatag gcgaaagatt gaatagattc
     1921 ttgcttttcc ttagcttctc tttgcatttt ggctaaatgc tcgaaagggt ctaagccttg
     1981 taaaacaagg gtatttagtt cgtctctctt taagcgtgct tcggctaagc tcatttgcgg
     2041 atagctaccg attctatttt ttgtgcttcg cttgcctgtt ttggggctga tatagctata
     2101 aatccatact ttcgcttttt tctgtgcaag aataaatagc ccgttgctgt ctgcgatagg
     2161 cttcgcattt ggggaatatt tgagattttt tagtaatgtg tctgaaagtg gttttttagt
     2221 tgcagtcatt tcttctaatc cttgatatat aagggtttat cgaagtttat gatccctaaa
     2281 ttttcccgtt ttcaaaatag ggatcatttt agggatcata aacactaaga tacaatgcaa
     2341 ctaaagaaga caataagaga ctataaaaga atctaagttc ttgattttat agtaaatttg
     2401 agacaatgaa agacaatatg caacgcttag atggtccccc cagccggact tgaaccagcg
     2461 accaagcgat tatgagtcgc ctgctctaac cactgagcta tggggggatt tgaaaagtgg
     2521 ttggaattat agagaaaaac cgagtagtcg tctagaggca gaagattact cggttaatta
     2581 ttgaacgaga gaaaataggg ttattctgtt cgccaaatta aggttgccat tcgcccggtt
     2641 tgtttgtctc ggcggtagga aaaaaacagt tctggttggc tgtaggtgca atattcaccg
     2701 cctgaaatgg cggtaatgcc taatttattc agacgttggg tggcaatttg gtaaagattt
     2761 cctaaaaatt taccgcttgt agtgctgtct tcaataaggg cttctttcgc tcttgggtca
     2821 aaagcacaga attggtctgc cacttcactc ccgacttgaa aagcattcgg tccgatagcc
     2881 ggccctagcc aagctgatat ttcgtgtggc gggcagttga atttctctac cgtcgcctct
     2941 aatatgccat cgcataaccc acgccagccg gcgtgtgccg ccgcaatttc cgacccgtct
     3001 tggctggcaa ataataccgg caaacaatct gcagtcatca ctaagcagac ttggtttggt
     3061 tgattggtgt aaatcgcatc ggctttaata ttatcacctg aatagggaag ttcaatcact
     3121 cgggtgctgt gagtttgggt taaaaacagc ggagcttgtg gtaattgaaa tttctcaacc
     3181 aataaagcac gattttgagc gatatcttgt ggctcgtcat taacgtgatc acctaagttg
     3241 aaactgtcaa aaggcggttt gcttactccg cctgttctta aggtcgtcag tgcgtgaata
     3301 tgtttaggca cattccaatt tggaataatt ttatccattt gcaaaaaatc tcaagaaaat
     3361 gaccgcttgt tatcggcgta ctgcaatcgc ttcgatctca atgccaacat cttttggtaa
     3421 acgagccact tctacgcaag aacgagccgg aaaattaggg tgttcgtttt cttggaagaa
     3481 ggcttggtat tccgcattta cctttgcgaa gtcgttaaga tctttgacaa atacggtggt
     3541 ctttacaata tcggcaacgg ttaagccggc ttgttcgata atggctttca cgttgttcag
     3601 agattggcgg gtttgttgca caatatcact cgggatttcg ccggtttcag gattcacggg
     3661 aatttgccca gaagttaaaa ccatattgcc taaatcaact gcttgcacat aaggcccaat
     3721 cgctgccggt gcattttctg tgtggataac tgtcatcttg tgttcctctt aatgaaaaat
     3781 aaacggatta atgataat
//
LOCUS       DANLRK010000199         3783 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.200, whole genome shotgun sequence.
ACCESSION   DANLRK010000199 DANLRK010000000
VERSION     DANLRK010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3783
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.200"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            1..2042
                     /locus_tag="PXA33_002343"
                     /pseudo
     CDS             1..2042
                     /locus_tag="PXA33_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249230.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
     gene            2086..3522
                     /locus_tag="PXA33_002344"
     CDS             2086..3522
                     /locus_tag="PXA33_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3327 domain-containing protein"
                     /protein_id="HDL5121638.1"
                     /translation="MKKTTACILTFLVSSVLYANNITIELKQNDFVQGKLIQKAQQPL
                     SLSIIFPDQSSRLLAQNVFGEQDFMFKSELIGTATFSILANGATVSDQDFTLTIERHI
                     PISAQIAPPKVIENTQLEELSQKIAKNPEKQTALINDFWDKAKQAGTPLIEPLDEQQS
                     RVTFLWRGATKNARIWGGVSTEHDFMQRFGETDIWYKSYIVPNDTLVEYKLAPDVPTL
                     PVDENTQRRALLATAQADPLNKTAYFEKIGQNIHNTDKFNYASLLKLPNAPTQSFLTE
                     APNIAKGKLQQLIFESATLGNKHRLFVYLPDGFSAEKNYAVLYLFDGVEYTERVPTKT
                     ILDNMIAQKVILPTVAVFIENPSSESRRVELPANPIFTQVLAKELVPFVEKQLNIKTK
                     ERIIGGSSYGGLSSAYTVLNYPNVFSKALILSGSFWWQPKGTPSEQSHYIAQQFIQKE
                     KLPLCFFISAGFYESGKSAICKRSNYFA"
     gene            complement(3664..3750)
                     /locus_tag="PXA33_002345"
                     /pseudo
     CDS             complement(3664..3750)
                     /locus_tag="PXA33_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558044.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         1236 a          771 c          712 g         1064 t
ORIGIN      
        1 ctttagcgat tgtagctcct caagtattag cagaaacaac ctctcaagca gaactaaatg
       61 ctattgaggt caaagagaaa ttggcagcta ccaccggcta tattcctgtt tatacgactg
      121 taggaacgaa aacattaacc tctttggctc gtacaccttt tagtatcaat gttgtctctg
      181 atgatttaat gcatgaccaa gctgttcgtt ctgttaccga ttcagtggct tatacctccg
      241 gtatgatggg tggctatcgt ggtaacaatt cgcttatcga aatctccatt cgaggcattg
      301 gcaataaaag tgatggcagt acaattccga cctatttaaa cggttctcgc taccaaactg
      361 catttgaaat cgatccgttc ttcttagaaa gtatcaatgt gattaaaggt ccaaactcca
      421 ttttatacgg gcaagctaat cccggtggcg taatggatat tatcaccaaa aaagccggca
      481 gtaacccaca tcgccatttg caatttgtgg tgggaaacca tcaacgatac caaatcgggc
      541 tggatattga agaaaatctc tccaatacct tatccgctcg tttaatcggt caattagaac
      601 gaacaaactg gaatacctaa tttgtaaaag aaaaaggcgg tgcttttgct ccaagcattt
      661 tatggaaacc gactacaaat acagaactta atgtttacgc ttactatgca aaaaaaccga
      721 aagccggtga tcgtaacttt ttaccgaaag aaggaacgat taatagcgta aacgggaagg
      781 aactacccta tgatatgttc ctcagcgatc tgaactatca cggtttaaaa gccgaacaaa
      841 tgcaagtagg ttacagtttc tcacataaat ttacgccaaa cctaacgttc cgccaaaatg
      901 caagttacca taatggcgaa gagaatttca aaaatctagt ttattggggc gatgtcggtt
      961 cggttttaga acgtagagct cgccattggg atgtcaaaac ccaagaaatc aacttagata
     1021 accaattaga atgggtattt agcacagcaa atataaatca taccttgtta gccggtattg
     1081 attataaacg agtgcgtaaa gatcttgata actatatggg gattgcccct gattttgatt
     1141 ggcagaaccc tgtttatggc gtgaccgtta ctgagcctag cttattttca agtgaagtga
     1201 agcatttaaa acaacttggg gtttatctac aagatcaaat agaaatcggt aattttgact
     1261 tcctgttagg cggtcgttac gatcgagcaa aaaacagcaa ccaagatcgt ttgaacagta
     1321 aaacagagaa taaccaagaa ggaaaattta cttggcgtac aggcgtagtt tacaactttg
     1381 aaaacggtat cgctccgtat atcagctaca gcacttcttt tgtgccatcg gctgaaaaag
     1441 atgctcaagg aaatttctta aaaccaacaa ctgctgggca atgggaagtg ggaatgaaat
     1501 accaacctac ccaagacacc ttactgacct tagcaggatt taaaattaca caaaaagatt
     1561 tagccaatta tcaatggcaa acccgctctt atgagcaaat tggtgaagtg caaaccaaag
     1621 gaattgaagt gagtttaaat cagcaattat ccgataaatt ttcaatagcg gcttcttatg
     1681 cttacttaaa aaaagagatt actgcagata aagatggcac gacgattgga aaaacgcagt
     1741 ggggagtgcc tcgccatcaa gcatctcttt gggcaaaata taaattcttt gataagctga
     1801 ccgccggtgt aggtgtacgc tatatgggaa cgacttttgg cgataaccaa aatactttta
     1861 ccgtaccggc atacacactt tacgatatga tgctagctta tgattttatg cctaacttaa
     1921 acttacagct caacgcacaa aacctgacga ataaaaaata tgtcgcaagc tgtgccaata
     1981 atttttcctg tttctatgga aaagatcgca atttaagtgc aacgttaaac tatcattttt
     2041 aatctcaaca gccctcaaat gagggcttaa acttttgcaa aaaatatgaa aaaaacgacc
     2101 gcttgtattc ttactttttt ggtttcatct gtgctttatg ctaacaatat aaccattgag
     2161 cttaaacaga atgattttgt gcaaggcaaa ttgattcaga aggctcaaca gccactttcc
     2221 ttgtcgatta tttttcctga ccaaagctct cgcctgctgg ctcaaaatgt ctttggtgaa
     2281 caagatttta tgtttaaatc agaactaatc ggcacagcta ctttttctat tctagcgaat
     2341 ggtgcaaccg tttcagacca agattttacc ttaacgattg agcggcatat tccaatctcg
     2401 gcacaaattg ctccacctaa agtaattgaa aatacacagc ttgaagaact cagccaaaaa
     2461 attgcaaaaa atccagaaaa acagaccgct ttaattaacg atttttggga taaagcaaaa
     2521 caagccggca caccattgat tgaaccattg gacgaacagc aaagccgtgt aactttttta
     2581 tggcgtggag caacgaaaaa tgcccgtatt tggggcggtg tgagtactga acacgatttt
     2641 atgcaacgct ttggtgaaac ggatatatgg tataaaagtt atatagtgcc caatgacact
     2701 ttagtggaat acaaattagc tccagatgtg ccgacattgc ccgttgatga gaatacccaa
     2761 cgcagagcgt tactggctac ggctcaagcc gatccactca ataaaacagc atacttcgag
     2821 aaaatcggac aaaatattca taataccgat aaattcaact acgcttcgct acttaaattg
     2881 ccaaacgcac ctactcaatc ttttttgact gaagctccaa atattgcaaa aggtaaactg
     2941 cagcagttga tttttgagag tgcaacactc ggcaataaac acagattatt cgtctatctg
     3001 cctgacggtt ttagtgcaga aaaaaactat gcggttctct atttatttga tggtgttgaa
     3061 tataccgaac gagtgccaac taaaaccatt ttagataata tgatcgctca gaaagtgatt
     3121 ctgcctactg tagcggtgtt tatcgaaaat ccatcgagtg aatcaagacg tgtcgagctg
     3181 cctgccaatc ccatatttac gcaagtattg gcaaaagaac ttgtaccttt tgttgaaaaa
     3241 cagctcaata taaaaacaaa ggagcgaatt attggcggct ctagctatgg cgggctatcc
     3301 tctgcttaca ccgtactcaa ctatcctaat gtatttagta aagcattgat tctatccggt
     3361 tcattttggt ggcaacccaa aggcacacct agtgaacaaa gccactatat tgctcaacaa
     3421 tttatccaaa aagaaaaatt accactctgt tttttcatca gtgcaggttt ttatgaaagc
     3481 gggaaatcgg ctatttgtaa gcggtcgaat tattttgcat agaattacac tcaattttgc
     3541 atagataaaa attaaaaact atgcaaaata attgtagaag atcaaaccta atctgacagt
     3601 cccccgtttt aaattaccgt gtctgtcaga ttaatttgag cttaaattct tttccaccca
     3661 aacgcgtcta ttttgattca gcaatgcttc aacaccacct tgctcaaccg cttgttgata
     3721 acgatagaat gtatctcggc tcattcccat tactttgcag gcttgagaaa tattacccag
     3781 ttc
//
LOCUS       DANLRK010000200         3736 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.201, whole genome shotgun sequence.
ACCESSION   DANLRK010000200 DANLRK010000000
VERSION     DANLRK010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3736
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.201"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(48..821)
                     /locus_tag="PXA33_002346"
     CDS             complement(48..821)
                     /locus_tag="PXA33_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HDL5121639.1"
                     /translation="MNLSNALKLIKYNELDVLEVNHNKFKAKIALQGAQLLSWQPYNA
                     EQDVLWLSEIEPFRIGSAIRGGIPICYPWFGSIKEPAHGTARIQEWRLVEHTCSDENV
                     GLVFALENKAKMEMMLGETCELYFTHLDPEPAQLALHSYFNIGDIEKVEVQGLPTRCF
                     DKLTDQEVEVPSPRKISENVDCIYPAQAVNFIQDFANQRTIKVEHINATETVLWNPWH
                     NTVSAMTETAYQNMVCVETARINTLLQQNETLGVRVLVR"
     gene            970..>3736
                     /locus_tag="PXA33_002347"
     CDS             970..>3736
                     /locus_tag="PXA33_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S6"
                     /protein_id="HDL5121640.1"
                     /translation="MKTKTFTRSYLASFVTIVLSLPAVASVVRNDVDYQYFRDFAENK
                     GPFSVGSMNIDIKDNNGQLVGTMLHNLPMVDFSAMVRGGYSTLIAPQYLVSVAHNTGY
                     KNVQFGAAGYNPDSHHYTYKIVDRNDYEKVQGGLHPDYHTPRLNKLVTEVVPAAVTNA
                     GTSIKPYLNEERFPMFLRAGSGTQALRGKESNKTTGIAGAYEYLTGGTTLQLSKSSPD
                     HWLDYSSNLYQVSYGPLSTYALPGDSGSGSYAYDMNEKRWVLVGVLNFYNGMDNQFNR
                     SAIIRKDFHEKKFAEDIAGTINNTVQNAQFNWTAQGKSSSLSQSNNVQKLNVDLKDSS
                     IANQNTSLPQENHGKTINFNGKDATIVLKQDIDQGAGALNLNANLTIRPETDQTWQGA
                     GIIVGKDKKVNWQVKNPQGDRLSKLGEGTLYVNGRGQNLGDISVGDGIVILNQQADHQ
                     GRKQAFNTVGIVSGRPTVVLGSADQVNPDNIYFGFRGGRLDLNGNSIAFKRIQNSDKH
                     ARIVNHNRDHISTLIIQGQDPLTSNDLIWGKWASNSPADIYEYTNPYQNKRKDYFRLK
                     GNSRVYYPTNATSNDHWEFLSSNREQAIQKILDAKNLRQRYDTFNGFIGEDASNKTNG
                     ILNVVFDTKTEVNTEQDKLKNIYTMSGGFNLNGELTLKGGTLLLSGHPTPHAYDIKNK
                     HDVVRENDWQDSHFTAKNITVNKMAQLYIGRNVNEVNSHFTATDKAKLNLGFINRSTP
                     SCYDSEYTGTTHCEVQAVISDNIFANLATTAIKGNVKLQNHSQLNLGKANLTGSVQAD
                     QTTHITLANHSHWLNNGTSQIGHLTMEKGSILSLNDKFATTEIPVRFNKMIIQGNLKG
                     NGRINYTANLAKGESDHLQVDGIAEGNFVLAVRNSTTEANPKSSLNLLSLKNSNQEGN
                     KASISLEN"
BASE COUNT         1266 a          736 c          660 g         1074 t
ORIGIN      
        1 aatttgtaaa caatattact aattcagctg cttgctctca ttaaacacta tcgcactaaa
       61 accctcactc ctagcgtttc attttgttga agtagagtat ttatccgagc ggtttccaca
      121 cataccatat tttgatacgc cgtttctgtc atcgcactaa cggtattatg ccaaggattc
      181 caaagtacgg tttcggtcgc attaatatgc tcaactttaa tggtgcgctg atttgcaaaa
      241 tcttgaataa aattcaccgc ttgtgccgga taaatacaat ccacattttc acttatcttg
      301 cgaggtgatg gcacttcaac ttcttgatcg gttagcttat caaaacagcg agttggcaag
      361 ccttgcactt ccactttttc aatatcacca atattaaaat aagagtgcaa tgccagttgt
      421 gctggttctg gatctaaatg ggtaaaatat aactcacaag tttcgcctaa catcatttcc
      481 attttcgctt tattctctaa ggcaaatacc aaaccgacat tttcatcaga acaagtatgc
      541 tcaactaaac gccattcttg aatgcgagct gtgccgtggg caggctcttt tattgaaccg
      601 aaccacggat aacaaattgg tataccgcca cgaatcgcag agccaattct aaacggttct
      661 atctcactta accacagcac atcttgctct gcattatacg gctgccaact tagcaattgt
      721 gctccttgta atgcaatttt agctttgaat ttattgtgat taacttctag cacgtctaat
      781 tcattatatt tgattaattt taatgcatta ctcaagttca taatttattc ctttgatgac
      841 taaaataatt gacagcaaac actacttagt aattttatat aaaataaggg aagacatctt
      901 gccaaactat cagttataag acagaatagc cagaattttt tattacttat aaggataaac
      961 ttctcattta tgaagaccaa aacatttact cgttcttatc ttgcttcttt tgtaacaatc
     1021 gtattaagtt tacctgctgt agcatctgtt gtacgtaatg atgtggacta tcaatacttc
     1081 cgcgattttg ccgaaaataa aggaccattt tcagttggtt caatgaatat tgatattaaa
     1141 gacaacaatg gacaacttgt aggcacgatg cttcataatt taccaatggt tgattttagt
     1201 gctatggtaa gaggtggata ttctacttta attgcaccac aatatttagt tagtgttgca
     1261 cataatactg gatataaaaa tgttcaattt ggtgctgcag gttataaccc tgattcacat
     1321 cactatactt ataaaattgt tgaccgcaat gattatgaaa aggttcaagg agggttgcac
     1381 ccagactatc atactcctcg attaaataaa ttagtaacag aagttgtgcc tgccgcagtc
     1441 accaatgcag gtacatctat taaaccctac ttaaatgaag aacgcttccc tatgtttctt
     1501 cgtgctggtt cagggacaca agcgctaaga ggaaaagaaa gtaataaaac aactggaatc
     1561 gctggtgctt atgaatatct tactggcggt accacattac aattatctaa aagctcccct
     1621 gatcactggt tagattattc aagtaacctt tatcaagtaa gctatggacc actttcaacc
     1681 tatgcactac ctggtgatag tggttcaggt tcttacgcct atgatatgaa cgaaaaacga
     1741 tgggtattag ttggtgtgct caatttctat aatggtatgg ataatcaatt caaccgctct
     1801 gcgattatcc gtaaagattt ccacgagaaa aaatttgccg aagatattgc aggaacaatc
     1861 aataataccg tacaaaatgc acaattcaat tggactgctc aaggtaaatc cagctctctt
     1921 agtcaatcaa ataatgtgca aaaactcaac gttgatctaa aagatagtag cattgcaaac
     1981 caaaacactt ctctgccaca agaaaatcac ggtaaaacca ttaattttaa tggtaaagat
     2041 gcaactattg tactaaaaca ggatattgac caaggtgcag gtgcattaaa tctgaacgct
     2101 aatctcacta ttcgtcctga aacagaccaa acttggcaag gtgcaggtat tatcgtcggt
     2161 aaagataaaa aagtgaattg gcaagtaaaa aatccacaag gcgatcgttt atctaaactc
     2221 ggggaaggaa cactctatgt aaatggacgt ggacagaatc ttggcgatat cagtgtaggt
     2281 gatggtattg taatacttaa ccagcaagcc gatcaccaag gaagaaaaca ggcctttaat
     2341 acagtaggaa tcgtaagtgg tcgccccact gttgtgctag gtagtgcaga tcaagttaat
     2401 cccgataata tttactttgg atttcgcgga ggtcgtttag acctaaacgg taacagcatc
     2461 gcctttaaac gtattcaaaa cagcgataaa catgctcgta ttgtaaacca caatcgcgat
     2521 cacatttcta ccttaataat acaaggccaa gatcctctca ctagtaatga tcttatatgg
     2581 ggaaaatggg caagtaatag cccagcagac atttacgaat ataccaatcc ttatcaaaat
     2641 aaacgcaaag attacttccg tctgaaaggt aattcgagag tatattatcc aacgaatgct
     2701 acaagtaacg atcactggga atttctttcc agtaaccgcg agcaagcaat acagaaaatc
     2761 ctagatgcca aaaacttaag acagcgctat gacacgttta atggttttat aggggaagat
     2821 gcttccaata aaactaatgg gatattaaat gtcgtgtttg atacaaaaac agaagtaaat
     2881 acagaacaag ataaattaaa gaatatctac acaatgtcgg gaggatttaa ccttaatggt
     2941 gaactcaccc ttaaaggtgg tacattgttg ctttctggtc acccaacgcc acacgcttat
     3001 gatattaaga ataagcatga tgttgtgcgt gaaaacgatt ggcaagacag ccattttact
     3061 gctaaaaata tcacggtaaa taaaatggca caactctata tcgggagaaa tgtcaatgaa
     3121 gtaaatagtc actttactgc gactgataaa gccaaactca atttaggatt tattaatcgt
     3181 tcaacgccaa gttgctatga ttctgaatac acaggcacta cacattgtga agtgcaagcg
     3241 gtcatttccg ataatatttt tgcaaatcta gcaacaaccg ccattaaagg taatgttaaa
     3301 ttacaaaacc atagccaatt aaatttaggc aaagcaaacc tcactggttc tgtacaagct
     3361 gatcaaacaa ctcatatcac tttagcaaat cacagtcact ggttaaacaa tggtacgagc
     3421 cagattgggc atcttacaat ggaaaaaggg tcgatcctta gcctaaacga taaatttgct
     3481 accacggaaa tcccagtccg attcaacaag atgatcatcc aaggtaatct aaaaggtaat
     3541 ggacgaatta actataccgc aaatttagcc aagggcgaat ctgatcatct ccaagttgac
     3601 ggtattgctg aaggaaattt tgtccttgcc gttagaaata gcacaactga agcaaatcca
     3661 aaaagctcat taaacctact aagcttaaaa aatagcaacc aagaaggcaa taaagcttct
     3721 atttctctag aaaata
//
LOCUS       DANLRK010000201         3724 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.202, whole genome shotgun sequence.
ACCESSION   DANLRK010000201 DANLRK010000000
VERSION     DANLRK010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3724
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.202"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            <1..573
                     /gene="nfuA"
                     /locus_tag="PXA33_002348"
     CDS             <1..573
                     /gene="nfuA"
                     /locus_tag="PXA33_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010944401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HDL5121641.1"
                     /translation="SISDAAQAHFARLLEQQEEGTNIRIFVVNPGTPGAECGVSYCPP
                     NAVEETDTEFKFNRFSAFVDDISLPFLDEAEIDYVTDPMGSQLTLKAPNAKMRKVADD
                     APFIERLDYVIQTQVNPQLASHGGKVTLIEITEDKYAVLQFGGGCNGCSMVDVTLKEG
                     IEKQLLAMFPDELVGVKDITEHQHGEHSYY"
     gene            complement(741..2378)
                     /gene="pyrG"
                     /locus_tag="PXA33_002349"
     CDS             complement(741..2378)
                     /gene="pyrG"
                     /locus_tag="PXA33_002349"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HDL5121642.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLASILEARGLNVTIMKLDPY
                     INVDPGTMSPTQHGEVFVTEDGAETDLDLGHYERFIRSKMSKANNFTSGKIYSEVLRK
                     ERRGDYLGATIQVIPHITNEIKARVLEGGKGRDVAIVEVGGTVGDIESLPFLEALRQL
                     AVDIGREKTLFMHLTLVPYIPTAGEVKTKPTQHSVKELLSIGIQPDVLICRSDRAIPE
                     NEKKKIALFCNVPERAVISLKDVDSIYRIPELLKSQGLDSFVCDRFRLDCKEADLSEW
                     EQVLYCQANPTGEVTIGMVGKYVELPDAYKSVNEALKHAGLTHRLTVNIKYIDSQDIE
                     TKGTELLAGLDGILVPGGFGYRGVEGKIRTAQYARENKIPYLGICLGMQIALIEYARN
                     VAGLTEANSSEFDKDCSQPVIGLITEWQDAEGNVETRSDESDLGGTMRLGSQQCHLIE
                     GSKARELYGAETIVERHRHRYEVNNKLLPQIEAAGLKVSGLSADRKLVEIIEVPNHPW
                     FIAAQFHPEFTSTPRDGHPLFAGFVKAAKDHQESRKA"
     gene            2642..3691
                     /gene="ansB"
                     /locus_tag="PXA33_002350"
     CDS             2642..3691
                     /gene="ansB"
                     /locus_tag="PXA33_002350"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HDL5121643.1"
                     /translation="MKIKKLLLPTLLSIATMPIQAADLPNITILATGGTIAGSQKNTA
                     TSAYQAGQLNIETLIEAVPDIKKLANIKGEQIVKIGSQDMSDEVWLKLAKAINQQCPN
                     TDGFVITHGTDTMEETVYFLDMTVKCEKPVVLVGAMRPATEKSADGVLNLYNSVVVAK
                     DSKSSGRGVLVAMNDIVLGARDVTKTSTTSVQTFNSPNFGALGYIHNSKVDYERSPES
                     KHTVNTPFKVDELTELPKVGIIYAYSNMPTEPLQALLDASYQGIVVAGVGNGNMNAAN
                     LALLEEAAKKGITVVRSSRVPTGYTTRDAEIDDSKYGFIASGTLNPQKARVLLQLALT
                     QTNDIKTIQQYFEDF"
BASE COUNT         1145 a          818 c          722 g         1039 t
ORIGIN      
        1 tcaatatcag atgcagcaca agcccatttt gccagactat tagaacagca agaagaggga
       61 actaatatcc gtatttttgt ggtcaatccc ggcacaccgg gggcagaatg cggtgtctct
      121 tactgcccac ccaatgcagt tgaagagacg gataccgaat ttaaatttaa ccgtttctct
      181 gcatttgtcg atgatattag cttaccgttt ttagacgaag cggaaattga ttatgtaacc
      241 gacccaatgg gttcacaact taccctcaaa gcccctaacg ccaaaatgcg taaagtggct
      301 gatgatgcac cgtttatcga gcgtttagat tatgtgattc aaacccaagt caatccacaa
      361 cttgccagcc acggcggtaa agtgacctta attgaaatca ccgaagataa atatgccgtg
      421 ctacaattcg gcggtggttg taacggttgc tcaatggtgg atgtgacctt aaaagaggga
      481 attgagaaac agcttctggc aatgttccct gatgaattag ttggtgtgaa agatattacc
      541 gaacaccaac acggtgaaca ttcttactat taaaaatact aaaagggata aatcattgat
      601 ttatcccttt cttataatta atgagcaagc ggtcaaattt actcgctttc gctcgcactt
      661 cgtgccatca gcaaagccga tgttcaaacg caagcgtttg tacaaaaaaa tgagtaaatt
      721 cgaccgcttg ttaagtttga ttaagcctta cgactctctt ggtgatcttt cgcagctttc
      781 acaaaacctg caaataatgg atgaccatca cgcggagttg aggtaaattc cggatggaat
      841 tgtgctgcta taaaccaagg gtgatttggc acttcaataa tttccactaa tttacgatct
      901 gccgataaac cgctcacttt taagcctgcc gcttcaattt gtggtaacag cttattgttt
      961 acttcgtaac ggtggcgatg acgttcaaca atcgtttccg caccatataa ctcacgagct
     1021 tttgagcctt caattaaatg gcattgttgt gagcctaaac gcattgtacc acctaaatct
     1081 gactcatcgc tgcgggtttc cacattccct tccgcatctt gccattcggt aattaagcca
     1141 atcaccggct gtgagcaatc tttatcaaat tcagaagaat ttgcctcggt taaacctgcc
     1201 acattacggg catattcaat tagggcgatc tgcataccta aacagatacc taagtaagga
     1261 atcttgtttt cacgggcata ttgtgcggtt cgaattttgc cttccacgcc acgatagcca
     1321 aagccgcccg gcacaagaat gccgtctaag cctgccaata actctgtgcc tttggtttca
     1381 atatcttgtg aatcaatgta tttaatattc actgttaaac gatgcgttaa acctgcgtgt
     1441 ttcaaagcct cattcaccga tttatacgca tcaggtaatt ccacatattt tccgaccata
     1501 ccgatagtca cttcgccggt tgggtttgct tggcagtata gcacttgttc ccactctgaa
     1561 agatcggctt ctttgcaatc taaacggaaa cgatcgcaca caaaggaatc taacccttgc
     1621 gatttcaata attccggaat gcgataaatc gaatctacat ctttaagcga aataaccgca
     1681 cgctccggca cattacagaa aagggcgatt tttttcttct cattttccgg aatagcacga
     1741 tctgaacggc aaattaacac atccggctga ataccgattg aaagcaactc ttttactgaa
     1801 tgctgagtcg gcttagtttt cacttcccct gcggttggaa tataaggcac taaggttaaa
     1861 tgcataaaca aggttttttc acgaccgata tccaccgcta attggcggag tgcttctaag
     1921 aatggcagtg attcaatatc ccccaccgtg ccacctactt ctacaatggc gacatcacgc
     1981 cctttaccgc cttctaacac acgggcttta atctcgttgg taatatgcgg aataacctga
     2041 atggttgcac ctaaatagtc gccacggcgt tctttacgca acacttcaga gtaaatttta
     2101 ccgctggtaa agttgttggc tttgctcatt tttgaacgga taaaacgctc gtagtgacct
     2161 aaatccaaat cggtttccgc accgtcttcg gtcacaaata cctccccgtg ctgagttgga
     2221 ctcattgtac ccggatcgac attgatataa ggatcaagct tcataatggt tacatttaaa
     2281 ccacgagctt ccagaataga tgctaaagag gctgccgcaa tacccttccc taaagaagat
     2341 acaacaccgc ctgtgacgaa aatataattc gttgtcattg tgattgcctt tgctgattga
     2401 ttaggttgga aattcagaat atcagtttac ttcgtaaaaa agaaactatc aggacgggaa
     2461 agtagtttac atcaaattac atttttttgc taatggaaaa tcggcaagca gtcagattaa
     2521 ttcttttttc aataatgccg actaatctta attaatacac tattatttgc gatagatcag
     2581 aattaaccta catgtttata gatatcataa tacctgtttt tctaatactg gagacgatac
     2641 aatgaaaatc aaaaaattac tactgccaac tctgttaagc attgctacta tgccaattca
     2701 agcagccgac ctacctaata ttacaattct tgcaactgga gggacaattg ccggtagcca
     2761 aaaaaatacg gcaacctcag cttatcaagc tgggcagcta aatattgaaa cactcataga
     2821 agccgtcccg gatattaaaa aattagcaaa tatcaaagga gaacaaatcg ttaaaatcgg
     2881 atctcaggat atgtctgatg aagtatggct aaaactggca aaagcaatta atcaacaatg
     2941 cccaaacaca gatggcttcg ttattacgca cggcacagac actatggagg aaaccgtata
     3001 cttccttgat atgactgtaa aatgcgaaaa acccgtagta ttagtggggg ccatgcgacc
     3061 agcaactgaa aaaagtgccg acggcgtatt aaatttatat aactcagttg ttgtcgccaa
     3121 agattctaaa tctagtggca gaggggtgtt ggttgcaatg aatgatattg tattgggcgc
     3181 tcgcgatgtt acaaaaacca gtactacatc agtgcaaaca tttaactcac caaactttgg
     3241 cgcactaggc tatatccata atagcaaagt tgattatgag cgctctcctg aaagtaaaca
     3301 caccgtaaac acccctttca aagtagatga attaacggaa ttgccaaaag tcggcattat
     3361 ttatgcttat tcaaatatgc caaccgaacc gcttcaagca ttattagatg cgagttatca
     3421 aggcatcgtg gttgcaggag ttggcaatgg caatatgaat gcggctaatt tggctttact
     3481 tgaagaagca gctaaaaaag gaattaccgt ggttcgctcg tctcgagtgc caaccggata
     3541 tactacccga gatgcagaaa ttgatgatag caagtatggt tttattgctt ccggcacatt
     3601 aaatcctcaa aaagctcgag tattactgca gctcgcctta acacaaacaa atgatataaa
     3661 aactattcaa caatattttg aagatttcta aaaagtaaat aacaagcggt ctattttgca
     3721 aaac
//
LOCUS       DANLRK010000202         3668 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.203, whole genome shotgun sequence.
ACCESSION   DANLRK010000202 DANLRK010000000
VERSION     DANLRK010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3668
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.203"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            178..1101
                     /gene="oppB"
                     /locus_tag="PXA33_002351"
     CDS             178..1101
                     /gene="oppB"
                     /locus_tag="PXA33_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HDL5121644.1"
                     /translation="MLKFIFKRLLEAIPTLLILITFSFFLMRLAPGSPFTSEKAYPPK
                     VMANIEAKYHLNEPLYKQYFLYMENLSKGDFGPSFKYKDQSVNNLIASAFPVSIKLGI
                     TAFIFAVLIGLTAGTIAALKQNSKWDYLVMGFSMTGIIMPSFVFAPLLVLMFAIYLKW
                     LPAGGWNGGQLYYMVLPVLSLTISYVAGIARITRGSMIEVLHSNFIRTAKAKGLPMRK
                     IIFKHALRPALLPVITYLGPAFVGIITGSMVIESVFGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGTLTILFNAIVDILYAVIDPKIRYS"
     gene            1116..2015
                     /gene="oppC"
                     /locus_tag="PXA33_002352"
     CDS             1116..2015
                     /gene="oppC"
                     /locus_tag="PXA33_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HDL5121645.1"
                     /translation="MQNSSKNQPLVEVTSTIQGRSLWQDARRRFFRNKAAVASLIILC
                     CIILFITFAPMLMPFSYEDTDWNMMGIAPDFESQHYFGTDSSGRDLLVRVAIGGRISL
                     MVGIAGALIAVLIGTIYGAISGYFGGKVDMVMMRFLEILSSFPFMFFVILLVTFFGQN
                     IFLIFIAIGMVAWLNLARIVRGQTLSLKNKEFVEAAIVCGVSRRQIIWRHIVPNVLGL
                     VVVYASLEVPSLILFESFLSFLGLGTQEPMSSWGALLSDGAAQMETSPWLLAFPAFFL
                     CLTLFCFNFIGDGLRDALDPKDK"
     gene            2025..3620
                     /locus_tag="PXA33_002353"
     CDS             2025..3620
                     /locus_tag="PXA33_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL5121646.1"
                     /translation="MELLAVNNLDVYLKTDEELVHAVRNVSFSIEKGQTLAIVGESGS
                     GKSVTSMAIMQLLPKNIVSFGERSSIIFEEQNLLTLSEKAMQTVRGDRIGMIFQEPMT
                     SLNPYMPIGKQVAETVQTHNPQISNAEAEKMVLETLQKVKIPDAEKKMICYPHEFSGG
                     QLQRIMIAMAIINKPDLLIADEPTTALDVTTQAEILDLMHDLQRDMGMAIILISHDLR
                     LVHKYSDLVCVMQNGEIIERGETETVFTNPQHPYTVELLTPIPTNLKGELPPNAPTLL
                     QGEHIKVDYILKRSLFGKPKKVFNALKDISLHLKTGETLGIVGESGSGKSTLGRAIMQ
                     ILDYRGNITFAGKTLANLNKAERQALKKDMQMVFQDPFNSLSPRLTVGEIIAEGLTVH
                     CPQMSKTERREKVMKMLEEVNLNPAMINRYPHEFSGGQRQRIAIARAIILEPKFVLLD
                     EPTSALDRSTQITVIELLNRLQKKYGLSYIFISHDLAVIKALSDRVIVMSQGEIVESG
                     DVTQIFENPQQEYTKRLILASNL"
BASE COUNT         1019 a          732 c          791 g         1126 t
ORIGIN      
        1 aaatcagcac tttaaagagt attaaagtgc cgattttttt acaaaaataa gctaaaaaaa
       61 caaccgctta agaataatga attcttataa aaatagccat aactaatcaa tatctttaca
      121 aagccgttaa tttacgctat taattgccta ccaatataac taaaaagagt ggtgcttatg
      181 ctgaagttta tttttaaacg tcttcttgag gcgattccaa ccctactaat tctgattacc
      241 ttttcctttt tcctgatgcg gcttgccccg ggtagcccat ttaccagtga gaaagcctat
      301 ccgccgaaag taatggcaaa tatcgaggca aaatatcacc tgaatgaacc gctgtataag
      361 cagtattttc tttatatgga aaatctgtca aaaggcgatt tcggtccatc ttttaaatat
      421 aaagatcaat cggtaaataa tctgattgct tctgcgtttc ctgtttcgat aaaactgggc
      481 attactgcct ttatttttgc ggtgcttatc ggtttaaccg caggaactat cgctgcactt
      541 aagcaaaata gtaaatggga ttatttagtg atgggctttt caatgacggg tattattatg
      601 ccgagctttg tctttgctcc gctgttggta ctcatgtttg cgatttatct caaatggttg
      661 cctgccggtg gttggaacgg tggtcagctc tactatatgg ttttacctgt tctctcttta
      721 accattagct atgttgccgg cattgcccgt attactcgtg gttcaatgat tgaggtgttg
      781 cactctaatt ttattcgcac cgctaaggca aaaggcttgc caatgcgtaa aattattttc
      841 aaacacgctt tgcgtccggc attgttgcct gtgatcactt atttaggccc ggcttttgtt
      901 ggcattatta ccggctcgat ggtaattgaa agcgtgtttg gcttaccggg aatcgggcag
      961 ttgttcgtaa acggagcatt aaatcgtgac tattcattag tattaagcct aaccattttg
     1021 gtaggtacgc tcaccatttt atttaatgcg attgttgata ttttatatgc ggtcatcgac
     1081 ccgaaaattc gttattcata ggagtaaagc ggaaaatgca aaattcttca aaaaatcaac
     1141 cgcttgttga ggttacctca accattcaag gacgaagttt atggcaagat gcacgccgcc
     1201 gttttttccg aaataaggcg gctgtggcaa gtttgattat tctttgttgt attattcttt
     1261 ttattacctt tgccccaatg ctaatgccgt ttagctatga agataccgat tggaatatga
     1321 tggggattgc tccggatttt gaatctcaac actatttcgg tactgattct tcagggcgtg
     1381 atttgctggt tcgagttgca atcggtggac gcatttcgtt aatggtaggg attgccggtg
     1441 cattaattgc ggtgcttatc ggtacaatct atggagcaat ttccggttat ttcggcggta
     1501 aagtcgatat ggtgatgatg cgttttctgg aaattttaag ctcattccca tttatgttct
     1561 tcgtaatttt gctggttacg ttcttcgggc agaatatttt cctgattttc attgcaatcg
     1621 gtatggtagc gtggctgaac cttgcccgta ttgtacgtgg tcaaactctt tcgcttaaaa
     1681 ataaagagtt tgtggaagca gcgattgtgt gtggcgtttc ccgccgtcaa attatttggc
     1741 gacacattgt gcctaacgtt ttaggcttag tggtggttta tgcctccctt gaagtaccat
     1801 ctctgatttt gtttgaatcg ttcttaagtt tcttagggct tggtacacaa gagccgatga
     1861 gcagttgggg ggcattatta agcgacggag cggcacaaat ggaaacctcg ccttggctac
     1921 tggctttccc tgcctttttc ttatgtttaa cactgttttg ttttaatttt attggtgatg
     1981 gcttgcgtga tgccctcgat ccaaaagata aatagaggtt tagaatggaa ttattagcag
     2041 taaataatct cgatgtttat ttaaaaacgg acgaagaatt agttcacgca gtgcgtaatg
     2101 tctcgttctc gattgaaaaa gggcaaaccc ttgcgattgt gggggaatcg ggttcgggta
     2161 aatccgttac ttcaatggca attatgcagt tattgcctaa aaatatcgtc tcattcggtg
     2221 aacgctcaag catcattttt gaagaacaaa acttgcttac tctatctgaa aaagcaatgc
     2281 aaacagttcg aggtgatcgc atcggtatga ttttccaaga gccaatgacc tcactcaatc
     2341 cttatatgcc gattggcaag caggttgctg agacggttca aacccataat ccgcaaatca
     2401 gcaatgccga agcggaaaaa atggtgttgg aaactttgca aaaagtaaaa attcccgatg
     2461 cggaaaagaa aatgatttgc tatccgcacg agttttccgg cggtcagcta caacgaatta
     2521 tgattgcaat ggcaattatc aacaaacccg atttattgat tgccgatgag ccgaccaccg
     2581 ccctggatgt cacaacccaa gcggaaattt tggatttaat gcacgactta cagcgtgata
     2641 tgggaatggc gattatctta atttcccacg atttacgctt ggtgcataaa tacagtgact
     2701 tagtctgcgt aatgcaaaac ggcgaaatta ttgagcgagg cgaaacggaa actgtgttca
     2761 ccaatccgca acatccttat acggtggagc tacttacccc gattccaact aatttaaaag
     2821 gggaattacc gccaaatgca ccaaccttgc ttcagggtga gcatattaag gtagattata
     2881 ttttaaaacg ctcgctattc ggtaagccga aaaaggtgtt taatgcgtta aaagatattt
     2941 cattgcactt gaaaaccggc gaaacgctcg gcattgtagg cgaatccggc tccgggaaat
     3001 ctacccttgg gcgtgccatt atgcagattt tagattatcg cggcaatatt acatttgcag
     3061 gtaagacact ggctaatctg aataaagccg aacgtcaagc cctgaaaaaa gatatgcaaa
     3121 tggtgttcca agacccgttc aactcacttt ctcctcgctt aacagttggg gaaattattg
     3181 ctgagggctt aaccgtacat tgccctcaaa tgagcaaaac cgaacggcgg gaaaaagtaa
     3241 tgaaaatgtt ggaagaggtt aatctcaacc ctgcaatgat taaccgctat ccgcacgaat
     3301 tttcgggcgg acaacgccag cggattgcga ttgcaagggc aatcatttta gagccgaaat
     3361 ttgtattgct tgatgagcca acctcagcac ttgaccgctc tactcagatc actgtaattg
     3421 aattgcttaa ccgcttacag aaaaaatacg gcttaagtta tattttcatc agccacgatt
     3481 tggcggtcat caaggcactt agcgaccgag tgattgtaat gagccaaggt gagattgtag
     3541 aaagcggtga tgtcactcaa atttttgaaa atccacaaca agaatacacc aagcggttga
     3601 ttttagcctc taatttgtaa ccccttccac aaggtcttat tctaccaaaa aacaagacct
     3661 tgtcattt
//
LOCUS       DANLRK010000203         3617 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.204, whole genome shotgun sequence.
ACCESSION   DANLRK010000203 DANLRK010000000
VERSION     DANLRK010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3617
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.204"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(71..1573)
                     /gene="ppx"
                     /locus_tag="PXA33_002354"
     CDS             complement(71..1573)
                     /gene="ppx"
                     /locus_tag="PXA33_002354"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HDL5121647.1"
                     /translation="MSSNIAPREFAAIDLGSNSFHMIVARIVNGSIQVLSRLKRRVRL
                     ADGLDENLVLSQDAIDRGIACLALFAKRLEGFSAENVKVVGTYTLRRAINNQAFLEQA
                     KAVFPYPISIISGQQEARLIYSGVSHTQPETGRKLVVDIGGGSTEMTIGDDFTPIRAE
                     SRHMGCVSFAKRFFPQGELTIERFDSAYQLAMEKIEDLMWEYKELGWDYALGSSGTIK
                     TVHQVLIANGYRDGLITESRLQKIIDICLEFKSLNEIKLRGLLDERADVLVPGLAILL
                     ALFHTFRIDVLRYSNGALREGVMYGLEKSFQVGDIRQRTAEALAEQFALDKQQAKRVE
                     HTALILFDQIKTWKNRRQVQDLRSMLKWASLLHEIGIVVNHNNVHRHSAYLIANRDLP
                     GFDFEQQTLLSVLMRYHLKGFKRSDIRSINRYQCRDVLTLVRVFRLAVLLNRSRQATV
                     VPKQLNLVIENNAWQLTFEQGFLANNPLISIDLEEEQKILASTNLQFAVS"
     gene            1769..2098
                     /locus_tag="PXA33_002355"
     CDS             1769..2098
                     /locus_tag="PXA33_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HDL5121648.1"
                     /translation="MSEVNKSFHDMLEYVHLYRLKNKLHRETADNDRKIRDNQKRVLL
                     LDNLTQYITDEMSIEEVRAIISNMRDDYENRVDDYMIRNAELSKQRREIRQKMASHKQ
                     APTNKKA"
     gene            complement(2162..3592)
                     /locus_tag="PXA33_002356"
     CDS             complement(2162..3592)
                     /locus_tag="PXA33_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIBC"
                     /protein_id="HDL5121649.1"
                     /translation="MSTLTYLQKIGRALMVPVAALPAAALLMGIGYWIDPDGWGTNSQ
                     LAALLIKSGAAIIDNMSILFAVGVAFGMSKEKDGAAALSGLVGYLVITTLLSPGTVAQ
                     LKGIAVDQVPAAFGKINNQFIGILVGVISAEIYNRFYQVELPKALSFFSGKRLVPIVV
                     SFILLYVWPVIFGALVSFGESIKDMGALGAGIYAFFNRLLIPVGLHHALNSVFWFDVA
                     GINDIPNFLGGAKSLAEGKAVIGVTGMYQAGFFPMMMFGLPAAALAIYHSAKPAKKMQ
                     VASIMFAAALASFFTGITEPLEFAFMFVAPVLYVVHALLTGLSVYIAASMEWIAGFGF
                     SAGLVDLVLSTRNPLAVQWYMLLAQGIVFAVIYYVLFRVVIQAFNLKTPGREEDSEVQ
                     TVNTVSRSEQAAKFVEALGGKENITSIDACITRLRLTLNDTAKADETKLKALGSKGNI
                     KLGENGYQVILGPEAEFVADAMKKLN"
BASE COUNT         1158 a          839 c          635 g          985 t
ORIGIN      
        1 tttgaccgct tgttcgcaaa agggaatgca ataaacgaaa aaggaagcct tcgcttcctt
       61 catttaaatg ttaagagacc gcaaactgaa gatttgtact cgccaatatt ttttgctctt
      121 cttctaaatc tattgaaatc agtggattat tcgctaagaa gccctgttca aaggtaagtt
      181 gccaagcatt attttcaatc accaaattca actgtttcgg cactacagtc gcttggcgag
      241 aacggtttaa tagcaccgct agccggaaaa cccgtaccaa cgttaataca tctctacatt
      301 ggtaacggtt gatactacga atatccgagc gtttgaatcc tttaaggtgg tatcgcatta
      361 ataccgatag aagggtttgc tgttcaaaat caaatcccgg taaatctcgg tttgcaatca
      421 aataagcaga atgacgatgc acattattgt ggtttacaac aataccaatc tcgtgtaata
      481 aacttgccca tttcagcatt gaacgcagat cttgcacttg gcggcggttc ttccaagttt
      541 tgatttggtc aaataaaatc aacgccgtat gctccaccct ttttgcttgc tgtttatcta
      601 aagcaaattg ctcagctaac gcttctgcag tccgttggcg aatatcccca acttggaaac
      661 ttttctccaa accatacatt accccttctc ttaaagcccc gttggaataa cgtaatacat
      721 caattctaaa agtgtgaaaa agtgctaaaa gaatagctaa gcccggaact agcacatctg
      781 ctcgttcatc aagtaagcct cgtagcttaa tctcatttaa cgatttaaac tctaggcaaa
      841 tatcaataat tttctgtagg cggctttctg taattaagcc atcacgatag ccattggcaa
      901 tcagcacttg atggacggtt ttaatcgtac cggaagaacc taaagcatag tcccaaccta
      961 actctttgta ctcccacatt aaatcttcga ttttttccat tgcaagctga taggcactat
     1021 caaaacgctc aatcgttaat tccccctgcg ggaaaaagcg ttttgcaaaa ctcacacaac
     1081 ccatatgacg actttccgct cgaatcgggg taaagtcatc accaatagtc atttccgttg
     1141 aaccaccgcc aatatctacc actaattttc tgccagtttc gggttgggta tggctaacgc
     1201 ccgaataaat cagcctcgcc tcttgttgtc cggaaataat ggaaatcggg taaggaaata
     1261 ctgctttcgc ttgctccaga aaagcttggt tattgattgc tctacgtaag gtataagtgc
     1321 caaccacctt cacattctct gcagagaacc cttctaaacg ttttgcaaat aaagctaaac
     1381 aagcaatgcc tctatcaatc gcatcttggc ttaatactaa gttttcatca agcccatctg
     1441 ccagccgtac acgccgtttc aaacgggaaa gcacttgaat ggaaccattt acgatacggg
     1501 ctacaatcat atgaaagctg ttcgagccta aatcaatagc tgcaaactct ctaggagcaa
     1561 tatttgatga cataatgaaa ccctgtacca ataactgaaa taagtaagat attaccattt
     1621 ttgagcccta gatatcacta caaatttagc gttattcgcg agaattaatt taaatcataa
     1681 aatgtgataa atttttttga cgaattgacg gaaaaagaat aggatttacg agcttactta
     1741 attcaacact acatcaaggg agattgatat gtctgaagtg aataaatcat tccacgatat
     1801 gctggaatat gtgcatcttt atcgtctaaa aaacaaactg caccgtgaaa cggcggataa
     1861 tgatcgcaaa attcgagata atcagaagcg tgtactttta ttagataacc taacgcaata
     1921 tattaccgac gaaatgtcta tcgaggaagt tcgagcgatt atttccaata tgcgtgatga
     1981 ctatgaaaat cgagtagatg attatatgat ccgtaatgcg gaactttcca aacaacgccg
     2041 tgaaatccgc caaaaaatgg cctctcacaa acaagcacca accaataaaa aagcataaaa
     2101 agccaagcgg tctgatttgc aaaaaaatat gcaaatcaga ccgcttgtga ttaagcagag
     2161 attaattcag ctttttcatt gcatctgcca caaattctgc ttccggtccc aaaatcactt
     2221 ggtagccatt ctcgcctaat ttgatattac cttttgaccc taacgctttc aatttggttt
     2281 cgtctgcttt cgccgtatca tttaaggtta ggcgtaagcg ggtaatacaa gcatcaatgc
     2341 tggtaatatt ctctttacca cctaatgctt caacaaattt ggcagcttgt tcgctacgag
     2401 aaacagtatt cactgtttgt acttcgctat cttcctcacg ccccggcgtt tttaaattaa
     2461 aggcttggat cactactcgg aacaatacat agtaaattac tgcgaatacg ataccttggg
     2521 ccaataacat ataccactgc actgccagcg gatttcgtgt cgataacact aaatcgacca
     2581 accctgcact aaaaccgaaa ccggcaatcc attccattga ggctgcaata taaacagata
     2641 atccggttaa taatgcgtga accacataaa gcacaggtgc aacaaacata aaggcaaatt
     2701 caagcggttc cgtaatgccg gtgaagaacg aagctaaagc agcagcaaac ataatagagg
     2761 ctacttgcat tttcttagcc ggtttcgcac tgtggtaaat agctaatgct gccgcaggta
     2821 agccaaacat catcatcggg aagaaaccag cttgatacat acctgttact cctatcactg
     2881 ctttgccctc agccaatgat tttgccccac ctaagaagtt tggaatatca ttaatgcctg
     2941 caacatcaaa ccagaaaacc gagtttaatg cgtggtgtaa tccaaccgga attaataaac
     3001 gattaaagaa agcataaata cctgccccta acgcccccat atctttgata gattccccaa
     3061 aagagactaa agcaccaaaa ataaccggcc agacataaag caagataaat gacaccacaa
     3121 tcggcaccaa tcgtttaccg ctaaagaaag acaatgcttt cggtaactct acttggtaga
     3181 aacggttata aatttcggct gaaataaccc ctactaagat cccgataaat tggttgttga
     3241 ttttaccgaa tgctgcaggc acttgatcaa ctgcgatccc ttttagctga gcaacggtcc
     3301 ccggagacaa caaagtggta attactaaat acccgactaa accggataat gctgctgccc
     3361 catctttttc ttttgacata ccaaatgcca cacccacggc aaatagaata gacatattat
     3421 caataattgc cgccccagat ttaatcagca atgccgctaa ttggctattt gttccccagc
     3481 cgtccggatc aatccaatag cctataccca tcaataatgc tgctgctggt aatgcagcaa
     3541 ccggcaccat taatgcacgc ccaatttttt gtaaataggt taatgtactc ataaaagcct
     3601 ccaaaaatac ctcataa
//
LOCUS       DANLRK010000204         3528 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.205, whole genome shotgun sequence.
ACCESSION   DANLRK010000204 DANLRK010000000
VERSION     DANLRK010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3528
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.205"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            <1..353
                     /locus_tag="PXA33_002357"
     CDS             <1..353
                     /locus_tag="PXA33_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDL5121650.1"
                     /translation="NKADFQRVINAAGYSMKGLKAAYINEAAFRQEIWCATILFPLGL
                     ILGETNIEKALLVGTVLLVLVTELLNSAVESVVDRIGADFHELSGRAKDIGSAAVFMA
                     MVLLAITWILILFC"
     gene            390..2033
                     /locus_tag="PXA33_002358"
     CDS             390..2033
                     /locus_tag="PXA33_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HDL5121651.1"
                     /translation="MRLKKTLLSVALLSATSAMAYETGKTYRFTVLHTNDIHGHFWQS
                     DKGEYGLAAQKTLVDNIRKEVAEKGGSVLMLNAGDANTGVPESDMQNARPDIEGMNAI
                     GYDAMVLGNHEFDYPLQILSMQEKWAKFPFISANVINKKTDKPIVKPYVMLERGGLKI
                     AVIGLTTEDTAKLGNPDVTENAFFKNPIETAKNTLAEINQKEKPDVRIALTHMGYYFD
                     GKHGTNAPGDVTMARTLEKGAFDLIIGGHTHDTVCIDEKGQFKAKYTPGEECKPDFQN
                     GTWIVQAGEWGKFVGRADFEFKDGKATLVKYELIPVNLKKTIKKEDGTKEYRLYQPEI
                     KADEATYTLLKKYQDEGDKKLNIEVGRVKGLLDGKREHIRFQQTNLGRLIAQSQMERV
                     KADIGIMNSGGIRTSLEEGVITYKHILTVQPFGNMIATVDLKGQELLDTLTTIALKEV
                     DTGAYPQFAGISMVVDRTAKTISDVKIGGKPFDKEKTYKVSLPDYVAAGGDGYPLLKK
                     HPSYVNTGFIDAEMLKKFFEENKIIEADKYDPKNQIIFK"
     gene            complement(2240..3109)
                     /locus_tag="PXA33_002359"
     CDS             complement(2240..3109)
                     /locus_tag="PXA33_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL5121652.1"
                     /translation="MSSRGKILNTISSLQNSLTKTEKKIAAAILSQPELLSQCSLSEV
                     AKQLDVGEATFIRFCRTLGFKGYTDFKLELAIELATQNQDNRVLLDTDVSESDTSKDI
                     AEKLKVSLDNVIEETINLLDFNVLEKVVEELRKAKRIFLFGVGSSGLTAEDAKHKLMR
                     IGLQTDAVTNNHFMYMQAALVKEGDLVIGISHSGYSEEIVKSLRFSRANKATTVAITH
                     NLRSPVTEEANYVLINGNRQGHMQGDSIGTKMSQLFVLDLIYTLLVKAEPENALKQKQ
                     KTLNVILEQRVKG"
     gene            complement(3119..>3528)
                     /locus_tag="PXA33_002360"
     CDS             complement(3119..>3528)
                     /locus_tag="PXA33_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HDL5121653.1"
                     /translation="ARLCKKLKSLDLVNLELGWQELENGIRMNVMSFETTPAEGKKAE
                     LHRKFIDIQLLIDGEEMIEYGLSQPDLSKYDEYRDEDDYQLTAEIDDKNNLILKPNMF
                     AIFLPYEPHKPGNSVNGNKTLKKLVVKVPVELI"
BASE COUNT         1129 a          682 c          642 g         1075 t
ORIGIN      
        1 ccaataaagc agattttcaa cgtgtgatca atgcagctgg ctactctatg aaggggttaa
       61 aagccgctta tattaatgaa gccgcctttc gccaagaaat ttggtgcgct actattttat
      121 tccctctagg gcttattcta ggcgaaacca atattgaaaa agcattatta gtaggcacag
      181 ttttacttgt tttagttaca gaactactca atagtgcggt tgaatctgtt gttgaccgca
      241 ttggggctga tttccacgag ctttctggta gagctaaaga tattggctct gcagctgtat
      301 ttatggcgat ggttttactc gctataactt ggatattaat tttgttttgt taactctatt
      361 tttcaaccaa cactcttact gaggtattta tgcgcttaaa aaaaacatta ctttcggtag
      421 cactacttag tgcaacatca gcaatggcct acgagacagg taaaacatat cgttttacgg
      481 ttttacatac taacgatata cacggtcact tttggcaaag tgataaaggc gaatacggtc
      541 tcgctgctca aaaaacattg gttgataata ttcgcaaaga ggtcgcagaa aaaggtggtt
      601 cggtattaat gctgaatgca ggtgatgcga atacaggcgt gccggagtca gatatgcaaa
      661 atgcacgtcc tgatattgaa ggaatgaatg cgattggtta cgatgcaatg gttctaggta
      721 atcacgaatt tgattaccca ctgcaaattc tctctatgca agaaaaatgg gctaaattcc
      781 cctttatttc ggccaatgtc attaataaaa aaacggataa acccattgta aaaccttacg
      841 tgatgttaga acgaggcgga ctaaaaattg ctgttatcgg attaacaacc gaagataccg
      901 caaaattagg taatccagat gtaacggaaa atgccttttt caaaaaccct attgaaacag
      961 caaaaaacac attagctgaa attaatcaaa aagaaaaacc cgacgtacgt atcgctttaa
     1021 cccatatggg ttactatttt gacggtaaac atggtactaa tgcaccaggt gatgtaacaa
     1081 tggcccgtac cttagaaaaa ggagcgttcg atttaatcat tggcggtcat acccacgata
     1141 ctgtttgtat tgatgaaaaa ggtcaattca aagccaaata tacgccgggt gaggaatgta
     1201 aaccagactt ccaaaacggt acttggattg tgcaagccgg cgaatggggc aaatttgttg
     1261 gacgggctga ttttgagttt aaagatggaa aagctacctt ggtaaaatat gaattaattc
     1321 cggtcaactt aaaaaaaacc attaaaaaag aagacggtac taaagaatac cgactttacc
     1381 agcctgaaat caaagccgat gaagcaacct ataccttatt gaaaaaatat caagatgagg
     1441 gcgataaaaa gcttaatatt gaagttggtc gtgtgaaagg gttattagac ggcaaacgtg
     1501 agcatattcg cttccaacaa accaatctcg gtcgtttaat tgcacaatcg caaatggaaa
     1561 gggtaaaagc cgatatcggg attatgaact ccggcggtat tcgtacctcc cttgaagaag
     1621 gtgtaataac ttataaacat attctgactg ttcaaccttt cggtaatatg attgcaacgg
     1681 tggatttaaa aggacaagaa ttgttagata ccctcaccac tattgctctg aaagaagttg
     1741 atactggcgc ttatcctcaa tttgccggca tttcaatggt agtcgaccgt acagcaaaaa
     1801 ctatttctga tgttaaaatc ggtggcaaac cttttgataa agagaaaact tataaagttt
     1861 ctctgcctga ctatgttgcg gctggtggcg atggctaccc attactgaaa aaacacccta
     1921 gctatgtcaa tacaggcttt attgatgccg aaatgctgaa aaaattcttc gaagaaaata
     1981 aaattattga ggcagataaa tacgatccaa aaaaccagat cattttcaaa tagagctata
     2041 gaaaatgaaa ctcactctgg cagtctctgc tcaaaagagc tgtggctgcc tgtagattgt
     2101 tttcttactt tcaagtgtta taggaaatcg ataaaaaatc cctaccaaat taattcatca
     2161 ccatatgatg aaaattaaaa aacctctggc aatactttgc caaaagtctt tgacttttat
     2221 agggtaatat cactgcttat tagcctttaa cacgttgttc caaaatcaca ttcagtgttt
     2281 tttgtttctg tttcaacgca ttttcgggtt cggctttaac tagtaaggta taaattaaat
     2341 caagaacgaa aagttgtgac attttagtac cgatagaatc tccttgcata tgcccctgtc
     2401 gattaccatt aattaacaca taattagcct cttccgtgac gggggaacgt aaattgtgtg
     2461 taatcgcaac cgttgtcgct ttatttgcac gagaaaaacg tagtgatttc acaatttctt
     2521 cagaatagcc tgaatgacta atccctataa ctaaatcccc ctctttcacc aatgccgcct
     2581 gcatatacat aaaatgatta tttgtgactg catcggtttg tagcccaata cgcattaatt
     2641 tatgttttgc atcttctgcg gttaacccag aagaacccac tccaaataaa aagatccgtt
     2701 ttgccttacg taattcttca acaacttttt ccaacacgtt aaagtcaagc aaattaatag
     2761 tctcttcaat aacgttatct aaactcactt tgagcttctc agcaatatct ttcgatgtat
     2821 ccgattctga aacatccgta tcaagcaata ctctattatc ttgattttgc gtagccaact
     2881 ctattgcaag ctctaattta aagtcggtat aacctttaaa acctaatgta cgacaaaaac
     2941 gaataaaagt tgcttcacca acatctaatt gtttggccac ttcagataat gaacattggc
     3001 taagtaattc cggctgagat aaaatagcag cagctatttt cttctctgtt ttggttaaac
     3061 tattttgtag cgagctaatt gtattaagaa tttttccccg actagacata gtagcctcct
     3121 aaatcaattc aacaggtact ttcaccacca gttttttcaa tgttttgttg ccattcaccg
     3181 aatttccagg cttatgtggt tcataaggga ggaaaatagc gaacatattt ggcttgagga
     3241 tcaagttatt tttatcatca atctcagctg ttaattggta gtcatcttca tctcgatatt
     3301 catcatactt agataaatct ggctggctta acccgtattc aatcatttct tcgccgtcta
     3361 tcaataattg aatatcgata aattttcggt gtaactctgc ttttttcccc tcagctggcg
     3421 tagtttcaaa tgacattaca ttcatgcgaa taccattttc taattcttgc cagcctaatt
     3481 ccaaattaac taaatctaaa ctttttaatt ttttacaaag gcgagcga
//
LOCUS       DANLRK010000205         3518 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.206, whole genome shotgun sequence.
ACCESSION   DANLRK010000205 DANLRK010000000
VERSION     DANLRK010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3518
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.206"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            <1..549
                     /locus_tag="PXA33_002361"
     CDS             <1..549
                     /locus_tag="PXA33_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HDL5121654.1"
                     /translation="LAVGGYSLTKYNDLMRAEEDINSVWGNVESAYQRRADLIPNLVN
                     TVKGQANFEKETLTSVIEARAKATAVTIDPSNATEEQMAKFQEAQQGVNSALSRLLVS
                     VEKYPELKAHEAFLNLQAQLEGTENRINVERNNFNEKVKEYNKQVREFPTKLAAMIMG
                     FKAKPQFKSEAGSDKAPTVNFN"
     gene            631..1089
                     /locus_tag="PXA33_002362"
     CDS             631..1089
                     /locus_tag="PXA33_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HDL5121655.1"
                     /translation="MGLFSFLSNKLPIDTQRIEQAIADLEQKTSAELRVVVERKAKIG
                     KTDNAAMLRASELFDELKMRETAEHNGVLIYLSFKPHYAAIIGDEGIHQKVDNDFWQK
                     IYATMCLDCQQGDYTQAICDAIKMVEAPLAHHFPYQADDKNELSNEVVVK"
     gene            1086..1943
                     /locus_tag="PXA33_002363"
     CDS             1086..1943
                     /locus_tag="PXA33_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HDL5121656.1"
                     /translation="MMKSLCKFLSIFNRLLLVCLALFSLNATAVTYPEPPNPFYYITD
                     YTKNTLSQQEWRTLEDALIANCAKTSSQIAVVIIPSTEGEAVSTYAHTLFNKWGIGRS
                     KNNENNGILLLIAKNDRKLFIATGRGLEGALPDAIASSIIRNDITPFFKQEQYAAGIA
                     HGLSAIMAAINGEYAPYASYGEEMQEFDDIDGLLFFLFAGIVIFLIFRPRGNSTYISP
                     SSMDQLGQILRESQRRGGGFGGGLGGGFDRGSGGFGGGFGGGFGGSRGSGDSFGGGSS
                     GGGGAGGSW"
     gene            complement(2058..2537)
                     /gene="ispF"
                     /locus_tag="PXA33_002364"
     CDS             complement(2058..2537)
                     /gene="ispF"
                     /locus_tag="PXA33_002364"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HDL5121657.1"
                     /translation="MIRIGHGFDVHAFGEDRPLMIAGVEVPYHTGFIAHSDGDVALHA
                     LTDALLGAAALGDIGKLFPDTDMQYKNADSRGLLIEAYRQVQEQGYQVGNVDVTIIAQ
                     APKMRPHIDKMRQVIADDLQCDISQVNVKATTTEKLGFTGRGEGIACEAVALLLKRA"
     gene            complement(2539..3222)
                     /gene="ispD"
                     /locus_tag="PXA33_002365"
     CDS             complement(2539..3222)
                     /gene="ispD"
                     /locus_tag="PXA33_002365"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HDL5121658.1"
                     /translation="MKRKIIAVIPASGVGSRMQAEKPKQYLQLKGKTILEHTVSIFLE
                     HPHVEKVIVAVARKDPFYSTLELLKSPKIQLVFGGETRADSVLNALQEINTENAWALV
                     HDAARPCLKRSDLDKLLQINDPQGGILAIPAVDTMKRANGTKIAHTEDRSTLWHALTP
                     QFFPAQALKQALSSAFANKLQVTDEASAMELAGYQPQLVSGRSDNIKITRPEDLALAE
                     FYLTHNLEN"
     gene            complement(3240..3518)
                     /gene="ftsB"
                     /locus_tag="PXA33_002366"
     CDS             complement(3240..3518)
                     /gene="ftsB"
                     /locus_tag="PXA33_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HDL5121659.1"
                     /translation="MRLLITFFALLLGFFQYSFWFGKNGWSDYQEVQEEIALLKVENT
                     KLTSRNKLIEAEIYDLKNGVDALEERARLEREMVKENEMFYRIVPKNK"
BASE COUNT         1019 a          750 c          730 g         1019 t
ORIGIN      
        1 cttgctgttg gcggttattc tttaacaaaa tacaacgatt taatgcgtgc agaagaagac
       61 atcaactccg tatggggaaa tgttgaatcg gcttaccaac gccgtgcgga tttaattcca
      121 aacttagtga ataccgtaaa aggtcaggct aactttgaga aagaaacttt aacctctgtg
      181 attgaagctc gtgcaaaagc aactgcggta accattgacc caagcaatgc aactgaagag
      241 caaatggcta aattccaaga agcccaacaa ggtgtaaact ctgctctttc acgcttattg
      301 gtttcagttg agaaataccc tgaattaaaa gcccacgaag cgtttttaaa tctacaagct
      361 caattagaag gtacggaaaa ccgcattaac gttgaacgca ataactttaa cgaaaaagtg
      421 aaagagtaca acaagcaagt gcgtgaattc ccgactaaat tagcggcaat gattatgggc
      481 tttaaagcaa aaccgcaatt taaatctgaa gcaggttctg ataaagcacc aaccgtaaac
      541 ttcaactaat tttagtttca atttattcat attaaaagca ataagctctt tcaaatccgg
      601 tgcttattgc tttattttta aggggaacaa atgggattat tttcttttct ctccaacaaa
      661 ttaccgatag atacgcaacg cattgaacaa gcaattgccg atttagagca aaaaacctct
      721 gccgaattgc gtgtggtggt ggaacgaaaa gccaaaatcg gcaaaaccga caacgccgct
      781 atgttgcgag caagtgagct tttcgatgaa ttaaaaatgc gtgaaacggc tgaacataac
      841 ggtgtgttga tctacctttc attcaagcct cactacgctg cgattattgg cgatgaaggc
      901 attcatcaaa aagtcgataa cgatttttgg caaaaaatct atgccacaat gtgtttggac
      961 tgccaacaag gcgattacac ccaagccatt tgtgatgcaa tcaaaatggt ggaagctccg
     1021 ctcgctcacc atttccctta tcaggctgat gataaaaacg agctttcaaa cgaggtggta
     1081 gtcaaatgat gaaatccctt tgcaaatttc tatctatttt caaccgcttg ttattggtgt
     1141 gcttggctct gtttagccta aatgccacag cggtgaccta cccagagccg cctaatccgt
     1201 tttattatat tacggactac accaaaaaca ccttgagcca acaagaatgg cggacattag
     1261 aagatgcctt aattgctaac tgtgccaaga caagttcgca aattgccgtg gtgattattc
     1321 caagcacgga aggcgaggca gtttcaacct atgctcatac cctgtttaac aaatggggaa
     1381 tcggacggag caaaaataac gagaacaatg gtatattgtt attaattgcc aaaaatgatc
     1441 gcaaactctt tattgccacc ggacgtgggc ttgaaggagc gttacctgat gcgattgcat
     1501 cgagcattat ccgcaatgac atcacgccat tcttcaaaca agagcaatat gcagccggta
     1561 tcgcccatgg cttatccgcc attatggcag ccattaacgg ggaatatgcc ccttatgcaa
     1621 gttatggcga agaaatgcaa gaatttgatg acatagacgg cttactcttt ttcttgtttg
     1681 ccggcattgt gattttccta attttccgcc cacgaggaaa cagtacctat attagtccca
     1741 gctcaatgga tcaactagga caaatactaa gagaaagcca acgcagaggt gggggctttg
     1801 gcggtggtct cggaggtggc tttgatcgag gctcaggtgg atttggcgga ggcttcggtg
     1861 gtggttttgg cggaagcaga ggcagtggcg acagcttcgg tggtggttca tcaggcggtg
     1921 gaggcgctgg aggaagctgg tagtttccgc ccaatttaaa acaatacaag cggtcatatt
     1981 tctcaaaaaa attgcgtatt tttcgctaaa cgcacaaaac gctaatgaaa ataagaccgc
     2041 ttcctcactt ctctaagcta tgcccttttt agtaacaatg ctacggcttc acaagctatg
     2101 ccttcacctc gcccggtaaa ccctaatttt tctgttgtag tggcttttac attcacttgt
     2161 gatatatcgc attgtagatc gtctgcgatc acctgtctca ttttatcaat atgcggtcgc
     2221 atcttcggag cttgagcaat aatggttaca tcgacgttac cgacttgata gccctgttct
     2281 tgcacttgtc ggtaagcttc aatcaacaaa ccacgactat cggcattttt gtattgcata
     2341 tcagtatcag ggaataattt gccaatatca cctaaagctg ctgcgccaag caacgcatct
     2401 gttaaagcgt gcagcgctac atctccgtca ctatgcgcaa tgaagcctgt atgataaggc
     2461 acttcaaccc cagcaatcat cagtggtcta tcttcgccaa aagcgtgaac atcaaaaccg
     2521 tgtccaatac gtatcatttt agttctccaa attatgtgta aggtaaaatt ccgctaaggc
     2581 taaatcttca gggcgagtga ttttgatatt atcacttcta cctgaaacca attgtggctg
     2641 gtagcctgca agctccattg cagatgcttc gtctgttacc tgaagcttat tggcaaaagc
     2701 agaggataat gcttgtttta atgcttgagc aggaaaaaat tgtggggtta aagcgtgcca
     2761 taaggttgag cgatcttctg tgtgtgcgat tttcgtgccg tttgctcttt tcatcgtatc
     2821 taccgctgga atggcgagaa tgccaccttg cggatcgtta atttgcaaaa gtttatctaa
     2881 atctgaccgc ttgaggcaag ggcgagccgc atcgtgaact aatgcccaag cattttcagt
     2941 gttgatttcc tgtaaggcat ttagcactga atctgctctc gtttctcctc caaaaacaag
     3001 ttggatctta ggggatttta ggagttccaa tgtggaataa aaagggtctt ttcttgccac
     3061 cgctacaatc actttttcaa catgagggtg ttcaagaaaa atagacactg tatgctctaa
     3121 gatagtctta ccctttaatt gtaaatattg ttttggtttt tctgcttgca tccggctacc
     3181 aactcctgac gccggaatta ctgcaataat ttttcgcttc ataatatatc aattatgtat
     3241 tatttgtttt ttggtacaat tcggtagaac atctcgttct cttttaccat ttctcgttca
     3301 agtcttgctc tctcttcaag tgcatctacg ccgttcttta aatcgtaaat ttcggcttca
     3361 attagtttat tgcgtgaagt aagctttgtg ttttctactt ttaataaagc aatttcttct
     3421 tgcacttctt gataatcact ccaaccattt tttccaaacc aaaagctata ttggaaaaag
     3481 ccgagtaata gggcaaaaaa agtaattaat aaacgcat
//
LOCUS       DANLRK010000206         3483 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.207, whole genome shotgun sequence.
ACCESSION   DANLRK010000206 DANLRK010000000
VERSION     DANLRK010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3483
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.207"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            368..745
                     /locus_tag="PXA33_002367"
     CDS             368..745
                     /locus_tag="PXA33_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HDL5121660.1"
                     /translation="MDKSQGVVSVNIRGGSGFGRANNAVGPNYMGMLAGKYQFNDSTW
                     IGALFGHSWRKISQNYKIGGGRKVTDSSIDLSKIDDLDKESSTTSPFNAAHLKQKPMS
                     RMAKLEYGDKYQTITLLPKSGSG"
     gene            1475..2251
                     /locus_tag="PXA33_002368"
     CDS             1475..2251
                     /locus_tag="PXA33_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HDL5121661.1"
                     /translation="MNYSATLSAYIHDLFTPFISYSQTHRAPNIKEIFFSNLGDYGVN
                     TALQPEQAKTTQFGINGFKERVFSEEDKLGFKVLFYNTHLKNYIYNVQHTAVGSAPSL
                     FIFHKNYEKPVNIKGIELEFSYDIGSFYGNLAYARQNTNQPVSFTDASSRVDAASHYG
                     FKTQGFGASKISALPRDYGSLELGTRWFDQRLQIGGVAKYYGKSKRIAIDEFNYTYYP
                     GTHIVKETHRLEETVNKQPIIVDFYVSYEPIKSLILKLEL"
     gene            2855..3349
                     /locus_tag="PXA33_002369"
     CDS             2855..3349
                     /locus_tag="PXA33_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL5121662.1"
                     /translation="MSRKYKFHEKSGAYFVSFATVYWVGVFVREIYFQVIVDALDYCR
                     KHKGMILYAYCIMPTHIHLLFQAENQNPSDLIRDFKTFTAKILIQRIKDNLQENRREW
                     LLWMFSRAAERRCNVKQYQFWQHHNQPIEIYSQKFFDEKLNYIHQNPVVSGFVSEPWE
                     WKYN"
BASE COUNT         1176 a          561 c          646 g         1100 t
ORIGIN      
        1 atattgaaca tgattaatat tttcgtcttg tgctatacta gactcgtgca ataaaggcac
       61 acttctttct tcagtgtctt tagcgttgcg atttaaattt aattacattt tttataggaa
      121 agacaataga aatgaagatc aaaaaatatg tatttcacct gacttataca acacttatct
      181 gccccttacc tagggtattc gcagaggaag gttatatgtt agatacggtt gaggttaaag
      241 cccaagcaat aaaaaaacag atgaagtttt cattaaaaca ggggcaataa gcacccgaga
      301 taaaatcttg gaatcaaacc aaagcttaga gactattgtt cgttctatgc ctggcgcatt
      361 tacacaaatg gataaatctc aaggtgtggt ttctgttaat attcgaggag gttctggatt
      421 tgggcgagct aataatgcag taggcccaaa ttatatggga atgctcgctg gcaaatacca
      481 attcaatgac tctacttgga taggagcatt atttgggcat agttggcgca aaatctctca
      541 aaattataaa ataggaggcg gacgaaaagt tacggatagc tcgattgacc ttagtaaaat
      601 tgatgatcta gataaagaaa gttctaccac ctcaccgttc aatgcagctc atttaaaaca
      661 aaaaccaatg agtcgtatgg ctaaattgga atatggcgat aaataccaaa ccatcacact
      721 attaccaaag tcaggtagcg ggtagacatt taaaaaacca aaacaaccag ttaaattatc
      781 gttttcactt gcccgagtca ccttggttag atgttcaagt ggcctatgcg gaaaatcaag
      841 gcaaacaaaa ctacaacaaa gggagctgaa ttaataataa atatttaatt caaggtttgg
      901 caattaacaa caaagcgaca accttaaatt tgagtaacac ctttaaagtg tctccgttta
      961 aagatggaga atgggaagca atattaggtt ttaatcgttt aaagaaccgc tatagtaaaa
     1021 atagacatcc tgcggaatta aactataact tagagaatgg cgaaaccagc gaaaacaacg
     1081 attctgtagg aatgacagcc gttcgtaaat cgctttatac ttcaactttc cagcctgatg
     1141 gcgaacagaa gttcgatact ttttatctcg acaatcactt taactatggt atatggggat
     1201 tggatataaa cgcaaatatt aataccgctc ggtttattgg tcagcgtttt aaatattttc
     1261 cttttgcgat tagagaattg gagtcagata ttagacaggc aagtagaaat aggcagtttg
     1321 agcatttaag gtcattacaa tctcaattag acaacttgca aaaaaacatt gtacatatat
     1381 tccagatgag gattttccta atgatcctga attgggagag tttaactgtc ctgatgtcaa
     1441 ttttcctcat acagcaacag gaaaacataa acgaattaat tattcagcaa ccttgtctgc
     1501 ttatattcac gatttattca cgccctttat tagttattct caaacacatc gagcccctaa
     1561 tattaaagaa atattcttct ctaatttagg tgactatggt gtaaataccg cactacaacc
     1621 agagcaagcg aaaacaacac agtttggtat taatggattc aaagaacggg tatttagtga
     1681 agaagataaa ttaggattta aagtgctatt ttataacact cacttaaaaa actatattta
     1741 taatgtacaa cataccgctg ttggtagtgc tcctagttta ttcattttcc acaaaaacta
     1801 tgaaaaacca gtaaatatca aagggataga attagaattt agttatgata taggctcttt
     1861 ctatggcaac ttagcttatg ctcgtcaaaa tacgaatcaa cccgtatcct ttacagatgc
     1921 aagttcaagg gtagatgccg cttctcatta tggttttaaa actcaaggtt ttggtgccag
     1981 caaaatttca gcgttaccaa gagactatgg ctcattggag ttagggacaa gatggtttga
     2041 tcaaagacta caaattggcg gagtggcaaa atattacgga aaaagtaaac gtattgcgat
     2101 agatgagttt aactatactt attatcctgg aacacatatt gtgaaagaaa ctcatcggct
     2161 tgaagaaacc gtaaataaac aacctattat tgttgatttt tacgtgagct acgaaccaat
     2221 caagtcgctt atattaaagt tagaactata aaatgtcttt gacaaaaagt acattgatcc
     2281 tcttgatgct aataatgatg cagcaagtca gagggtttac aattctgata tcagtgatga
     2341 acctatttat acgctcaata attatgcaag agggagaact atagtgttta gcacaagtta
     2401 taaattctaa atggggttgt cctagataag ctagcttaaa tttcctttaa ctaattattt
     2461 ggcatagtga ctaaaattga gtctgcacag taggttaaaa ccttataata taaggattta
     2521 acccagatgg tgcaagcgtc cacgcttgtg ccagttaatt cctgttctga aagtatttca
     2581 gtcataagat aaaagcctaa atcagccaag taaaatttgg tttatttggg cttttgtttt
     2641 tggcacaagc gtggacgctt gcgtcatcat tttatttatc catccattgc tatcatttca
     2701 gtatttcttt ggcttatcac aagcggttaa attttatcaa cactttgcaa gttttaatga
     2761 tttttgcgat ctagatcgaa aaagttatat tggcttgtgg aatttaattt tgaatagggt
     2821 atttttctta ttccttttta agttgagaaa ggctatgagc cgaaaatata aattccacga
     2881 aaagagcggt gcttattttg tcagttttgc aactgtttat tgggttggtg tgtttgtgag
     2941 agagatttat tttcaggtaa ttgttgatgc cttagattac tgcagaaaac ataagggaat
     3001 gattttgtat gcctattgta ttatgccaac tcatatccat ttattatttc aagcagaaaa
     3061 tcaaaatcct tctgatttaa tacgagattt caaaacattc accgctaaaa tacttattca
     3121 gagaattaaa gataatttgc aagaaaaccg tcgggaatgg ttgctgtgga tgttttctcg
     3181 ggcggctgaa cgacgttgta atgtaaaaca atatcaattc tggcaacatc ataaccaacc
     3241 tattgaaatc tattcgcaga aattctttga tgaaaagtta aattatattc atcaaaatcc
     3301 agtggtaagt ggttttgtgt ctgagccttg ggaatggaaa tataattgaa ataaactgct
     3361 tgtatttttg cgatctctgt cgaaaaattg tgaattttta cttgtctgtc ggagaatttt
     3421 gcgtaaattc tgcttattat gttttagtta aaggtattct atgagtagat aacccaaatg
     3481 aaa
//
LOCUS       DANLRK010000207         3476 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.208, whole genome shotgun sequence.
ACCESSION   DANLRK010000207 DANLRK010000000
VERSION     DANLRK010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3476
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.208"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(9..1469)
                     /gene="xseA"
                     /locus_tag="PXA33_002370"
     CDS             complement(9..1469)
                     /gene="xseA"
                     /locus_tag="PXA33_002370"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HDL5121663.1"
                     /translation="MSNILTVTQLNYSVRHLLEMEMSHIWLTGEISNFSQPVSGHWYL
                     TLKDDKAQVRCAMFKMKNVRVNFRPQNGMQVLVRASVTLYEPRGDYQLIIESMQPAGE
                     GLLQQQFEQLKAKLSAQGLFAQEHKKPIPTFVKTVGIITSSSGAALQDILHILQRRDP
                     SLSVVIYPALVQGKEATQDIVNMIELANRRNECDVLIVGRGGGSLEDLWCFNEESVAY
                     AIYHSQIPIISAVGHETDVTIADFVADLRAPTPSAAAELVSRDQNELKRQLQHQFDKV
                     NLAFDRVWNHKFNRFQQLPLRLNAQHPSKQIQSQQQRFDYMEYRLEQAIYALVAAKKQ
                     VGKELNVRLIHQHPQKQIERQSQMLKQLELKLHWQMEQQLSKKQQKWKNITQRFERNP
                     LPYQLYQQQTMVAKMSQRLAYSIEKIMLKEQRHFQELCTKLDGLSPLKILTRGYSITQ
                     TKDGKTLTSTENIERGEAIITQLKTGKIISQVVDFL"
     gene            complement(1620..1696)
                     /locus_tag="PXA33_002371"
     tRNA            complement(1620..1696)
                     /locus_tag="PXA33_002371"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1660..1662),aa:Met,seq:cat)
     gene            1906..3012
                     /gene="mltA"
                     /locus_tag="PXA33_002372"
     CDS             1906..3012
                     /gene="mltA"
                     /locus_tag="PXA33_002372"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HDL5121664.1"
                     /translation="MNWKAYKKAIAAASVVAIATVLVACSSSGQKGKTTSYDAEKAKF
                     GAKYNGRHFMPYGTHFQAGRLGLNAAPVNQLDFLQQLSNVRAYSSLADSYAIAYGKIS
                     RWISSGGNINDLAKYGISTRLMRGEDGYQNVLMTGYYSPVIKARRTPQGQYQHPIYAM
                     PSSKRFSRAEIYAGALQGQGLELAYSNSMLDNFLLGVQGSGYVDFGDGRLNYFAYAGQ
                     NGFKYASVGRLLVEDGEIPKEKMSVQAIREWGQRNPSRVQGLLERNPSYVFFKNDPTG
                     QVKGSAGVPLVPLASVASDRSIVPSGSVLLVEMPLIDSKGNWTGKHEMRLMVALDVGG
                     AVKGHHFDLYQGIGDKAGHIAGLMKHYGRVWVLN"
     gene            3025..3474
                     /locus_tag="PXA33_002373"
     CDS             3025..3474
                     /locus_tag="PXA33_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121665.1"
                     /translation="MLKKLSAIFLLLPSAVLANNLQNITASTEPKYKISEIDVRILIR
                     QLNNIEQCIYPELAKPGYQQIYANWNLAENLTMQYFEYQLLKELLGEENQKLMQNDTL
                     STEYFHLLHSRLNHQKANVDPEKCDTFKPRYKEIYKSMENALTKKNQ"
BASE COUNT          996 a          667 c          749 g         1064 t
ORIGIN      
        1 aatttcccct acaaaaaatc cactacttga cttattattt ttcctgtttt tagttgggtg
       61 ataatagcct caccacgttc aatattttct gttgaagtaa gcgttttacc gtctttagtt
      121 tgggtaattg agtagcctct ggtcaagatt ttcaatgggc taagtccatc aagtttagtg
      181 cataactctt gaaaatgtcg ctgctctttc agcattattt tttcgataga ataagccaag
      241 cgttgcgaca tttttgccac catcgtttgt tgctgataaa gttgataagg tagcgggttg
      301 cgttcaaacc gttgcgtaat gtttttccat ttttgctgtt ttttgctgag ttgctgctcc
      361 atttgccaat ggagtttcag ctccaattgc ttaagcattt gggattggcg ttcaatctgc
      421 ttttgcggat gttggtgaat taaacggaca tttagctctt tccccacctg ctttttggct
      481 gcaacaagtg cataaattgc ctgctcgagg cggtattcca tataatcaaa acgttgctgt
      541 tgcgattgaa tttgtttgct tggatgctga gcattcaggc gtaaggggag ttgttggaaa
      601 cggttaaatt tatggttcca aactcggtca aaggctaaat tcactttatc aaactgatgt
      661 tgtaattggc gtttcaattc attttgatct ctgctgacta attccgcagc agccgaaggc
      721 gttggtgcgc gcaaatctgc aacaaaatca gcaatggtca catcggtttc gtgtcccacc
      781 gcactgataa tcgggatttg tgaatgataa atggcataag cgacactctc ttcgttgaaa
      841 caccataaat cctctaatga accgccacct cgcccgacaa ttaatacatc acactcattg
      901 cggcggtttg ccagttcaat catattcaca atatcttgcg ttgcttcttt gccttgcacg
      961 agagccgggt aaataaccac tgataagctc ggatctcgtc gttgcaaaat atgtaaaata
     1021 tcttgtaatg cggctccgct tgaggaggta ataataccaa cggttttaac aaaagtagga
     1081 attggctttt tatgctcttg agcaaacaag ccttgagctg aaagttttgc tttaagttgc
     1141 tcaaattgct gctgtaacaa gccttcgccc gccggttgca tactctcaat aatgagctga
     1201 tagtcacctc gtggctcata gagtgtaaca cttgcccgca ccagcacctg cataccgttt
     1261 tgtgggcgaa aattcactct tacatttttc attttaaaca tcgcacaacg cacttgtgct
     1321 ttgtcatctt ttaaagtgag ataccaatgc cccgaaactg gttggctgaa atttgagatt
     1381 tcgccggtta gccaaatatg gctcatttcc atttcgagca aatggcggac ggaatagttg
     1441 agttgggtaa cagttaaaat attgctcata ataatcaaaa aagcgtaaat gtagtagaag
     1501 tgtaacgaga aatgaaagcg agaggaagct tttgcagtca acaagcggtt attttttgct
     1561 gttttttggc aaataaaacg aaaaccctcg atgtgattca ctatcgaggg tttggaatgt
     1621 ggttgcgggg gccggatttg aaccagcgac cttcgggtta tgagcccgac gagctaccaa
     1681 gctgctccac cccgcgtctg atgaggctat aatatatcaa tttgacataa tgacaagtaa
     1741 aatcatacaa aattgctcta agtgatgtaa attacaacag atatagtatc aaaatacgct
     1801 tattgttgta aaaacaatca aattatattc tggttttatt ttgtcgctgt ttttgctatt
     1861 cttctgttta tttttcacat tttattaacg atataaggat attcgatgaa ttggaaagca
     1921 tataaaaaag ctattgcggc agctagcgtg gtagcgattg caacggtatt agtggcgtgt
     1981 tcgtcttcag gccaaaaagg caaaacaacc tcttatgatg cggaaaaagc taaatttgga
     2041 gcaaaatata atggtcgtca ctttatgcct tatggcacac attttcaagc tggacgttta
     2101 ggattaaatg ctgctcctgt taatcaacta gactttttac agcagctttc taatgtcaga
     2161 gcctatagta gcttggcaga ttcttatgct atcgcttacg gaaaaatcag tcgttggatc
     2221 tcatcgggcg gtaatattaa tgatttagca aaatatggta ttagtactcg tttaatgcgt
     2281 ggcgaagatg gctaccagaa tgtgttaatg acagggtatt actcgccggt tatcaaagcc
     2341 cgtagaacgc ctcaggggca ataccaacac ccgatttatg cgatgccgtc tagcaaacgt
     2401 ttttcacggg cggaaattta cgcaggagca ttgcaaggtc agggcttaga actggcttat
     2461 agtaattcaa tgctcgataa cttcttatta ggtgttcaag gtagcggtta cgttgacttt
     2521 ggtgatggtc gcttaaacta ttttgcttat gcagggcaaa atggctttaa atatgccagc
     2581 gttgggcgtt tattagtaga agatggcgaa attccgaaag agaaaatgtc agtgcaggct
     2641 atccgtgaat gggggcaacg taaccctagc cgtgttcaag gtttgttgga gcgtaatcca
     2701 tcgtatgttt tctttaaaaa cgatcctacc ggtcaggtaa aaggctctgc cggtgtgccg
     2761 ttagtaccac ttgcatctgt tgcatcagat cgtagcattg tgccatcagg tagcgtgttg
     2821 ttggtcgaaa tgccgttgat tgatagcaaa ggaaattgga ccggcaaaca cgaaatgcgt
     2881 ttaatggtgg ctcttgatgt tggcggtgca gtgaaaggcc atcacttcga tttatatcaa
     2941 gggattggcg ataaagcagg ccatatcgca ggtttaatga aacattacgg ccgtgtttgg
     3001 gtattaaatt aatacagggt aactatgtta aaaaaactat ctgccatatt cttacttcta
     3061 ccgagcgccg tgcttgcaaa taatttgcaa aatataaccg cttcaactga gcctaaatat
     3121 aaaatctcag aaatagatgt cagaattttg atacgccagc tcaataatat tgagcaatgt
     3181 atctatcctg agttagctaa acccggttac cagcaaatct acgcaaattg gaatctggct
     3241 gaaaatttaa cgatgcaata ttttgaatat cagcttctaa aagagttgct cggagaggaa
     3301 aatcagaaac tgatgcaaaa tgatacgctt tctaccgagt atttccattt actgcattct
     3361 cgtttaaatc accaaaaagc aaatgtagat cccgaaaaat gcgatacatt taaacctaga
     3421 tataaagaga tttataaaag tatggaaaat gcactcacta agaaaaatca atagta
//
LOCUS       DANLRK010000208         3476 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.209, whole genome shotgun sequence.
ACCESSION   DANLRK010000208 DANLRK010000000
VERSION     DANLRK010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3476
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.209"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(23..145)
                     /locus_tag="PXA33_002374"
     CDS             complement(23..145)
                     /locus_tag="PXA33_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entericidin A/B family lipoprotein"
                     /protein_id="HDL5121666.1"
                     /translation="MKKLVLTILAAATFLTACNTVDGVGEDVEKAGDKIQQAAQ"
     gene            complement(225..1202)
                     /gene="rluD"
                     /locus_tag="PXA33_002375"
     CDS             complement(225..1202)
                     /gene="rluD"
                     /locus_tag="PXA33_002375"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HDL5121667.1"
                     /translation="MTQQKILTAEITADLLGARLDQALAQLFPDYSRSRIKVWIESDL
                     VKVNGNIVNKAREKVFGGETVEITAEIEEEIRFEPQDIPLNIVYEDDDIIVINKPKDL
                     VVHPGAGNPDGTVLNALLHYYPPIAEVPRAGIVHRLDKDTTGLMVVAKNIPAQTHLVT
                     ALQKRRITREYEAIASGIMTQGGKVDEPMARHPTKRTAMAVHPMGKPAVTHYRIMERF
                     RNYTRLRLRLETGRTHQIRVHMAHIAHPLLGDQLYGGRPRPPKGASDSFLSVLRGFQR
                     QALHATMLRLEHPITGEMMEWHAPLPDDFIELIQALKADYELYKDQLDY"
     gene            1343..2122
                     /locus_tag="PXA33_002376"
     CDS             1343..2122
                     /locus_tag="PXA33_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HDL5121668.1"
                     /translation="MRKFKSLATLVLAGLFVAGCSNSNKELEQSNVQDLYGKGQTYLQ
                     DGDYNSAIRYLEAVGTKGGQYSGLGEQTQLSLIYAQYKVGEYYKALDAAERFARSYPN
                     AANMDYVFYLAGLSNARLSDNFIQDFFGVNRASRAVDNVRNAYGNFQTIVQRYPQSQY
                     AQDAQNWMNYLFNRLAEHELAVVKFYDERNAYVAVVNRIEEMLRFYPNTLATKQALPY
                     LKEAYKQMNIPDAEQKTELLIQEYESKELPNPEKLKYGEQF"
     gene            2195..3226
                     /gene="tsaD"
                     /locus_tag="PXA33_002377"
     CDS             2195..3226
                     /gene="tsaD"
                     /locus_tag="PXA33_002377"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HDL5121669.1"
                     /translation="MRILGIETSCDETGVAIYDEDKGLVANQLYSQIDMHADYGGVVP
                     ELASRDHIRKTLPLIQEALKEANLQPSDIDGIAYTAGPGLVGALLVGSTIARSLAYAW
                     NVPALGVHHMEGHLLAPMLEENAPEFPFVALLISGGHTQLVKVDGVGQYELLGESIDD
                     AAGEAFDKTGKLLGLDYPAGVAMSKLAESGTPNRFKFPRPMTDRPGLDFSFSGLKTFA
                     ANTIKANLNENGELDEQTKCDIAHAFQQAVVDTILIKCKRALEQTGYKRLVMAGGVSA
                     NKQLRADLAEMMKKLKGEVFYPRPQFCTDNGAMIAYTGFLRLKNDEQTDLSISVKPRW
                     AMTELPPIN"
     gene            3308..>3476
                     /locus_tag="PXA33_002378"
     CDS             3308..>3476
                     /locus_tag="PXA33_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HDL5121670.1"
                     /translation="MNNYVISLTSAQERRKHIEAEFGKQNIPFQFFDAITPDLIKEKA
                     KAFNIDISNTNL"
BASE COUNT         1049 a          740 c          675 g         1012 t
ORIGIN      
        1 tcttagaatt cgaagctttt tgttattgtg cagcctgctg aattttatcg cccgcttttt
       61 caacatcctc accaacacca tcaacggtat tacacgcagt caagaaagtt gcagctgcta
      121 aaatagttaa tactaatttt ttcatagaaa gctccttttt tgttgtttac ttatgaaaac
      181 gatttcaata tagcaacttt acattattta agcaacacct aaacttaata atctaattga
      241 tctttataaa gctcataatc cgcttttagt gcctgaatca attcaataaa atcatcaggt
      301 aacggggcgt gccactccat catttcacca gtaatcggat gctctaatct caacattgtg
      361 gcgtgtaagg cttggcgttg gaagcctcgt aacaccgata aaaacgagtc actcgcacct
      421 ttaggtgggc gaggtctacc gccatagagc tgatcaccta ataatgggtg agcaatatgc
      481 gccatatgta cccgaatttg gtgagtacgc cctgtttcta atcttaaacg taaacgtgtg
      541 taattacgaa aacgctccat aatacgataa tgcgtaaccg ccggttttcc cattgggtga
      601 acagccattg ccgtacgttt agttgggtgg cgagccattg gctcatcaac ttttccgccc
      661 tgtgtcataa tgccgctggc aatcgcttca tattcacggg taatacgacg tttttgtaat
      721 gcggttacta agtgagtctg tgccggaatg tttttagcca ctaccattaa gcctgtcgtg
      781 tctttatcca atcggtgaac aatccctgct cgaggcactt ctgcaatcgg tggataataa
      841 tgtagcaaag cattcagcac tgtaccgtcc ggattaccag cccctgggtg aaccactaaa
      901 tccttcggtt tattaatgac gataatatcg tcatcttcat acacaatatt tagaggaata
      961 tcttgaggct caaaacgaat ttcctcttct atttcagcgg taatttcaac agtttcacca
     1021 ccaaatactt tttcccttgc tttattcacg atattgccat taactttcac tagatcactt
     1081 tcaatccaca ctttaatgcg tgaacgagaa tagtccggaa aaagttgtgc cagagcctga
     1141 tctaggcgag cacctagtaa atctgcagta atttctgctg ttaaaatctt ttgttgagtc
     1201 attaaaccat aaatcctaat tagtcatttg cttacttgcg tgatgttata tagaatagcg
     1261 agatgaactt cataagttat tcttagtcag aggacattgt acattaactt ttcgtcctag
     1321 acacttttta taggtaaaac atatgcgtaa atttaaatct cttgccacat tagtattagc
     1381 cggactgttt gttgcaggtt gttcaaattc aaataaagaa ttagaacaat ccaacgttca
     1441 agatctttat ggcaaaggtc agacctattt acaagatgga gattataatt ctgccattcg
     1501 ctacttagaa gcggttggaa ccaaaggagg ccaatattca ggacttggtg agcaaaccca
     1561 actaagttta atttatgctc aatataaagt tggcgaatac tataaagcac ttgatgccgc
     1621 agaacgtttt gctcgctctt acccgaatgc agctaatatg gattacgtat tctatctagc
     1681 aggattatca aatgcacgtc taagtgataa ttttattcaa gacttctttg gtgtaaatcg
     1741 cgcctctcga gccgtagata atgttcgtaa tgcttacggt aacttccaaa ctattgtaca
     1801 gcgctaccct caaagccaat atgcacaaga tgctcaaaat tggatgaatt acttatttaa
     1861 ccgtttagcg gaacacgaat tagccgttgt taaattttat gatgaacgta atgcctatgt
     1921 ggctgtagta aaccgtatcg aagaaatgtt gcgtttttac ccgaatacac tggcaaccaa
     1981 acaagcattg ccatacttaa aagaagcata taagcaaatg aatatcccag atgcagagca
     2041 aaaaacggaa ctcttgatcc aagaatatga aagcaaagag ctaccgaatc ctgaaaaact
     2101 gaagtatggc gaacaattct agtcgtacag agaataatgt gaggggcgtt cttcgcccct
     2161 tttggttttc taacttattt tgacttctcc aactatgcga attttaggta ttgaaacctc
     2221 ttgtgatgaa accggtgttg ccatttatga tgaagacaaa ggcttagtgg caaaccagct
     2281 ttatagccaa attgatatgc acgccgatta cggtggcgta gtccctgaac tggcttctcg
     2341 agaccatatc cgtaaaacgt tgccactaat tcaagaagcc ttaaaagagg ccaatctgca
     2401 accctcggat attgacggca ttgcctatac tgccggccca ggcttggtcg gggctttatt
     2461 ggtcggctca accattgccc gttcgctggc ttatgcttgg aatgttccgg cattgggcgt
     2521 tcaccatatg gaagggcatt tacttgcccc aatgttggaa gaaaatgccc ctgaatttcc
     2581 gtttgtggca ttattgattt caggtggaca cacccaactg gtaaaagttg acggcgttgg
     2641 gcaatacgaa ctactcgggg aatcaattga tgatgctgcc ggtgaagcct ttgacaaaac
     2701 aggcaaacta ctcggtttgg attaccctgc cggtgtagcg atgtcaaaat tagccgaatc
     2761 cggcacgcca aatcgtttta aattccctcg tccaatgacc gacagaccgg gactggattt
     2821 cagtttctcc ggtttaaaaa cctttgctgc gaatacgatt aaagccaatc ttaatgaaaa
     2881 tggtgaactc gatgagcaaa ccaaatgcga tattgcccac gcattccaac aagccgtggt
     2941 tgatactatt ttaattaaat gcaagcgagc gttagagcaa accggctata aacgcttagt
     3001 aatggcaggc ggcgtaagtg ccaataaaca attacgagca gaccttgcgg aaatgatgaa
     3061 aaaattaaaa ggcgaagtat tctaccctcg cccacaattt tgcactgaca acggcgcaat
     3121 gattgcctac actggctttc ttcgcttaaa aaacgatgaa caaaccgact taagcattag
     3181 cgtaaaaccc cgctgggcta tgaccgaatt accaccgatt aattaacctt tcaagcggtg
     3241 aaatttcttg ttaattttgc aaaaatttaa tcaaaaataa ccgcttgcta tatgatagat
     3301 taaatttatg aataattatg taattagcct aacctccgca caggagcgta gaaaacatat
     3361 tgaagctgaa ttcggcaaac aaaatattcc ttttcaattt ttcgatgcga ttacgccgga
     3421 tttaattaaa gaaaaagcca aagcatttaa tattgacatt tcaaatacaa acttaa
//
LOCUS       DANLRK010000209         3420 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.210, whole genome shotgun sequence.
ACCESSION   DANLRK010000209 DANLRK010000000
VERSION     DANLRK010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3420
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.210"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            17..1951
                     /gene="cpdB"
                     /locus_tag="PXA33_002379"
     CDS             17..1951
                     /gene="cpdB"
                     /locus_tag="PXA33_002379"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HDL5121671.1"
                     /translation="MLVLGTQPSAFAQTAKNKAGLRVIATTDIHGFLTDFDYYKDAPT
                     EKFGFTRTASLIKQARAEVKNSVLVDNGDLIQGNPISDYQSTKGAKEGKPEPSIMALN
                     AMQYEVGTLGNHEFNYGLSYLDNAIKQAKFPIVNANIVKPGTDEPFFTPYVIQQKEIV
                     DEKGNKHTIKIGYIGFVPPQVMIWDKANLEGKVEARDIVKTAQKYVPIVKKEGADIVV
                     ALAHTGPSDEPYKEGAENCAFYLADVKGIDAVIFGHSHRLFPNKEFANSPNADIAKGT
                     MKGVPESMAGYWGNNISVIDLSLAENDGKWGVVDGRAELRPIYNTEEKKATVENHPEI
                     VALLKETHEATRQFVSQPIGKATDNMYSYLALVQDDPTIQIVNQAQKAYVENVVKGLP
                     ELAGLPILSAGAPFKAGGRKNDPTGYTEVDKGELTFRNAADLYLYPNTLVVVKATGAE
                     LKEWLECSAGMFKQIDVASDKPQYLLDWTGFRTYNYDVIDGVSYQFDITQPARYDGEC
                     KLINPNSHRVVNLTFDGKPVDPKQEFLIATNNYRAYGAKFPGTGDKHIVFAAPDENRQ
                     VLANYITTESKAHGQVSPKADNNWRIAPISSNAKLDIRFETSPTDKAKAFIKENAQYP
                     MEYIENDETGFAVYRIDLTK"
     gene            complement(2024..>3420)
                     /locus_tag="PXA33_002380"
     CDS             complement(2024..>3420)
                     /locus_tag="PXA33_002380"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HDL5121672.1"
                     /translation="QYPEGSQFLYSTFTPRSNIYAPFVTRVVSFGFQAFILKYLIHYF
                     NDNFFSRSQHDVVSEYTQFISDTLNIEDSGEHIAQLHQLGYLPIRIKAIPEGKSVAVK
                     VPLMTIENTHSSFFWLTNYLETLINVSLWQPITSASIAFAYRKALAEFSEQTCDDNSH
                     LPFQAHDFSMRGMSSLESAESSGAGHLTSFLGTDTIPAISFVTAYYGSQHLIGTSIPA
                     SEHSVMSSHGVDELPTFRYLMAKYPNNMLSIVADTTDFWHNITVNLPLLKQEILARPE
                     SAKLVIRPDSGDFFTIICGNNSSPVEHERKGLIECLWDIFGGSINQKGYKVLNPHIGA
                     IYGDGVTYDKMIRILEGLTAKGFASSNIVFGVGAQTYQRNTRDTLGFAIKATSITING
                     VEKAIFKAPKTDNGLKKSQKGRVKVISYDHYVDELTALDDFSDDLLEVVFENGKLVKS
                     TNFDEIRQNIAQQF"
BASE COUNT         1137 a          653 c          700 g          930 t
ORIGIN      
        1 gcttggtgca actgcaatgc ttgtattggg gacacaaccg tctgcatttg cacaaacagc
       61 caaaaataaa gctggcttgc gtgtcatcgc cactacggat attcacggtt tcttaactga
      121 ttttgattat tacaaagatg ctccaacaga aaagtttggt tttacccgta cggcaagcct
      181 aattaaacaa gctagagcag aagttaaaaa cagtgtgtta gtcgataatg gtgacttaat
      241 tcaaggtaat cctatttctg actaccagtc aaccaaaggt gcaaaagagg gcaaacctga
      301 accttcgatt atggcattaa atgcgatgca atatgaagtc ggaacgctgg gaaatcatga
      361 atttaactac ggtttaagct atttagataa tgctattaag caagcaaaat tccctattgt
      421 caatgcaaac attgtgaagc cggggacaga tgaaccattc tttacacctt atgttattca
      481 acaaaaagaa attgtagatg aaaaagggaa taagcatacc attaagattg gttatatcgg
      541 ttttgtacca ccacaagtga tgatttggga taaagctaat cttgaaggaa aagttgaagc
      601 tcgagatatt gtgaagaccg cacaaaaata tgtgcctata gtaaaaaaag aaggggcaga
      661 tattgtcgtt gcattggcac ataccggccc ttcagacgaa ccctataaag aaggcgctga
      721 gaactgtgcg ttttatctag ctgatgtaaa aggtattgat gcagttattt tcgggcattc
      781 acatcgcctg ttcccaaata aagaatttgc aaattcacca aatgccgata ttgcaaaagg
      841 aacaatgaaa ggcgtaccgg aaagtatggc tggctactgg ggaaataaca tcagtgtgat
      901 tgatttaagc ttggccgaaa acgatggtaa atggggcgtt gtggacggta gagcagagtt
      961 gcgtccaatt tataatacgg aagagaaaaa agcaaccgtt gagaaccacc ctgaaatcgt
     1021 tgcattatta aaagaaaccc atgaagcaac ccgtcaattt gtgtctcaac ctatcggtaa
     1081 agcaactgat aatatgtata gttaccttgc gttagttcaa gatgacccta ccattcaaat
     1141 tgtgaaccaa gctcaaaaag cgtatgtaga aaatgtggta aaaggcttgc cggagcttgc
     1201 aggattacca attctaagtg ccggagcacc gtttaaagcc ggtggacgta aaaatgaccc
     1261 gacaggctat accgaagtcg ataaaggcga attaactttc cgcaatgcgg cagacttata
     1321 cctttaccca aataccttag tggtggttaa agcaacagga gcagaattaa aagagtggtt
     1381 agagtgcagt gcaggaatgt tcaaacaaat tgatgtcgct tcggataaac cgcaatattt
     1441 actggattgg acaggtttcc gcacctataa ctacgatgta attgacggtg tcagctatca
     1501 atttgatata acccaaccgg ctcgttatga cggcgagtgc aaattgatca accctaattc
     1561 gcaccgtgtg gtgaacttaa cctttgacgg caaaccggtt gatcctaaac aagaattctt
     1621 aattgccacc aacaattacc gtgcttatgg agcaaaattc ccgggaacag gcgataaaca
     1681 tattgtgttc gccgcaccgg acgaaaaccg ccaagtgtta gcgaattata ttaccaccga
     1741 aagtaaagct cacgggcagg taagtccgaa agcggataac aactggcgta ttgcaccaat
     1801 tagctcaaat gcaaaattgg atattcgttt tgaaacctct ccaacagata aagctaaagc
     1861 tttcattaag gaaaatgcac aatatccgat ggagtatatc gaaaacgatg aaaccggctt
     1921 cgcagtttac cgtattgatt taaccaaata atcagagcta aatacaagcg gtctaatttg
     1981 caaaaaaatt aacaaatttg accgcttgtt tcattccatt tttctaaaat tgttgtgcta
     2041 tattttgccg aatttcatca aaattggtcg atttgaccaa cttaccattt tcaaaaacca
     2101 cctctaaaag atcatctgaa aaatcatcca gtgcggttag ctcgtcaaca taatgatcat
     2161 aagagattac ttttactcgc cctttttgcg atttttttaa gccgttatcg gttttaggag
     2221 ctttgaaaat tgccttttcg acaccgttaa ttgtaattga agtcgctttg attgcaaaac
     2281 ccagcgtatc acgagtattg cgttgataag tttgagcccc aacgccaaac acaatattgc
     2341 tggaagcaaa accttttgcc gttaagccct ctaaaatccg tatcatttta tcataagtga
     2401 cgccgtcacc ataaattgcc ccaatatgcg gattaagcac tttataacct ttttggttaa
     2461 tactaccacc aaaaatatcc cacaaacact caatcagacc tttgcgttcg tgttcaacgg
     2521 gggaggaatt gttaccgcaa ataattgtaa aaaaatcacc gctatcaggg cggataacca
     2581 attttgctga ttccggtcga gctaaaattt cttgttttaa taatggcaaa ttaacggtga
     2641 tgttatgcca gaaatcggtt gtatctgcca caatagatag catattattt ggatattttg
     2701 ccattaaata acggaaagtc ggcagctcat caacaccgtg tgaactcatc accgaatgtt
     2761 ctgatgccgg aatagatgta ccgattaaat gttgagaacc ataataggct gtcacgaatg
     2821 aaattgcagg aatggtatct gtgcctaaaa atgaggttag atgtccggca ccggagcttt
     2881 ctgctgattc taaggaactc attccacgca tagagaaatc gtgtgcctgg aagggtagat
     2941 ggctattatc atcacaggtt tgctctgaaa attcggctag agccttacga taagcaaatg
     3001 caatggaagc tgatgtaata ggctgccata atgacacatt aatcagggtt tccaaataat
     3061 tagtcagcca aaaaaagctt gagtgagtgt tttcaattgt cattaaagga actttcactg
     3121 caacagattt tccttctgga atagctttaa ttctgatagg taagtaaccg agctgatgca
     3181 attgtgcaat atgttcgcca gagtcctcaa tattcaatgt gtcagaaata aattgagtat
     3241 attcagaaac aacgtcatgt tgtgagcgag aaaaaaaatt atcgttaaaa taatgaatca
     3301 agtattttaa aataaatgcc tgaaagccaa atgatacaac acgtgtaaca aatggagcat
     3361 aaatattact acgaggtgtg aatgtactat ataaaaactg ggaaccttca ggatattgaa
//
LOCUS       DANLRK010000210         3405 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.211, whole genome shotgun sequence.
ACCESSION   DANLRK010000210 DANLRK010000000
VERSION     DANLRK010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3405
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.211"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            23..2473
                     /locus_tag="PXA33_002381"
     CDS             23..2473
                     /locus_tag="PXA33_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014390755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HDL5121673.1"
                     /translation="MTVEVGGVVITADLEFENLLRDTQKVDKAMDDIGKSTLKAEKGL
                     KGLETQATKTAIAVNQATNKMKNIRGVAGQLGYQIQDISIMLQMGSNASTVIAQQGSQ
                     ILSIFGPMGAVAGAILAISGAIGGALLPSLFDSTDATELLAAAQKNLSEVVTQTESGV
                     SALSAKIIKLAKVSEDAAKAKIAVAMSDAKIAIKAAGDSSQKAFEQFDGFFGSFTNSS
                     QSIRNAIDELKRYESKGKDVGAVIKELGGTYGGAIAEVNALDSVTSEMSKTLGITQSE
                     ALGLIKLLAQLEQDKSPDNIKLLAIALSDLSDKNGWANEELNKLTRTINDNSVSALDA
                     ETSVRLLESAIKDLQNAIQNSQGALDGNVAKMNQLADAAERKAATIGKTERETAKYAA
                     TLLATTDEAKKNLDSTLSRIDASYDQIEAYQAEQKALQDKKTADAKAAREAEAAAKKS
                     AQAKKQVVEQLRQMATQYEIAVLRQKGMHLEAVKLEAGMRLGAAATKEQREEAEKLAQ
                     SIYGATAASQNFNAIQAQASPVTALDQRHQQQLAQIEEYKILYPQKIMEAEAARAAIE
                     EQYRQQRMEAQWEEWKQASDGARMFGDAVDAVGSSATSTITGLLNGTMSVRDAFASIA
                     NTILNSVVQSLVEMGMAQVKQMIMGQTAAKASMAAQMVQAKALAASFAPAASMVSLAT
                     QGANAIPAQAAITSTMSMAQVAAVTGRKLGGPVSARQMYRVGENNQPEIFKASNGMQY
                     MIPGQSGRVFSNKQSQGQGGGESKTVAVIVNQTNHFSSDEASANQAAVAKALSNQIKA
                     SVRLELQSQMRAGGVLAR"
     gene            2477..2806
                     /locus_tag="PXA33_002382"
     CDS             2477..2806
                     /locus_tag="PXA33_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL5121674.1"
                     /translation="MQTFNFKVEQDYTVQNEPSVVILKFADGYEQTAPKGLNHNLKKY
                     QGITVKANKAEAVKIQQFLNAHGGYKKFNWRDRTSDETVTVRCRSWSSTKLGAVIEFT
                     LNFDEVI"
     gene            2806..3276
                     /locus_tag="PXA33_002383"
     CDS             2806..3276
                     /locus_tag="PXA33_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HDL5121675.1"
                     /translation="MPKAPPKKMASELIKLEQDALIELWEIDLTHIYSSSNPNQRGEI
                     FRFHNGVSQTQQNIWWQGNEYQAYPIAADGFEISGQGPSNRPTLTVSNLYGVVTVLVE
                     DFGQPVEAKVSSEETVDGNIQMTVELLKKMDQIADQRYRRNQLNDLRTGGTLSK"
     gene            3284..>3405
                     /locus_tag="PXA33_002384"
     CDS             3284..>3405
                     /locus_tag="PXA33_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HDL5121676.1"
                     /translation="MPISISNQMKLDLAKLEQNAMLDLYEVDLRKLQDKNGNAG"
BASE COUNT         1158 a          671 c          804 g          772 t
ORIGIN      
        1 aaataatttt aaggaattgt ttatgacggt cgaagttggc ggagttgtaa ttaccgcaga
       61 tctagaattt gaaaatctgc taagagatac tcaaaaagtt gataaagcga tggatgacat
      121 cggaaaatca acattaaaag cagaaaaagg gctgaaaggg ctagaaacgc aggcaaccaa
      181 aacggcgata gcagtaaatc aagccacaaa caaaatgaaa aatatccgtg gagtagcagg
      241 tcaacttggt tatcaaattc aagacatctc aataatgctt caaatgggtt ctaacgcttc
      301 aaccgttatt gctcagcaag ggtcgcaaat cttaagtatt ttcggtccaa tgggagctgt
      361 tgcaggggcg atcttagcta ttagtggtgc tattggtggg gcgttactcc ctagtttgtt
      421 tgattcaact gatgcaactg agcttttggc agcagctcaa aaaaatctca gcgaagtagt
      481 tactcaaacg gaaagtggtg tttccgccct ttctgcgaaa atcattaaat tagcaaaagt
      541 aagcgaagat gcagcaaaag caaaaattgc agtagctatg agtgatgcaa aaatagctat
      601 taaggcagca ggggattcat cacaaaaggc atttgagcaa tttgacggct tttttggatc
      661 gtttacaaat tcaagtcaaa gtattcggaa cgcaattgac gaattaaaac gctatgaatc
      721 aaaaggtaaa gatgttggtg ccgtaataaa agaacttgga ggcacatatg gtggtgcaat
      781 tgccgaagtt aatgcgctgg attctgtgac aagcgaaatg tctaaaacgc ttggtattac
      841 tcaatctgag gctcttggtt tgataaaact gttggctcaa ttagagcaag acaaaagccc
      901 tgataatatt aaattactcg cgatagcttt atctgactta agcgataaaa atgggtgggc
      961 gaacgaagag ctaaataagc taaccagaac gataaacgat aattcagtat ctgctttaga
     1021 tgccgaaaca tcagtaagac tcttagagtc tgctataaaa gacttgcaaa atgcaatcca
     1081 aaactcccaa ggtgcattgg atggcaatgt agcaaaaatg aatcaacttg cagatgcggc
     1141 agaaagaaaa gctgcaacga taggcaagac tgagcgtgaa actgctaaat atgcagctac
     1201 gctattagct acaaccgatg aagctaaaaa aaatcttgat tccacattaa gtaggattga
     1261 cgcatcatac gatcaaatcg aagcctatca agccgaacaa aaagccttac aagataaaaa
     1321 gaccgcagat gcaaaagctg ccagagaagc tgaagcggct gcaaaaaaat ccgctcaagc
     1381 caagaagcaa gttgtcgaac agttgcgaca aatggctaca cagtacgaga ttgctgtttt
     1441 gaggcaaaag ggaatgcatc tcgaagccgt aaaattagaa gctggtatgc gtttaggagc
     1501 tgcggcaaca aaagagcaac gagaagaagc tgaaaaacta gcacaaagta tttatggagc
     1561 aaccgcagca agtcagaact ttaacgctat acaagcccaa gcatctcctg ttactgcatt
     1621 agaccaacga catcaacaac agctagctca gatcgaagaa tataaaatct tgtatccaca
     1681 aaaaataatg gaagcagaag ctgcgagagc tgcaatcgaa gaacaatacc gccagcaacg
     1741 aatggaagct caatgggagg agtggaagca agcgagcgac ggggcgagaa tgtttggtga
     1801 tgcggttgat gctgttggtt caagtgcaac aagcacaatt acggggctac taaatggcac
     1861 aatgtccgtg cgtgatgctt tcgcatcaat tgcaaatacg atcttaaata gtgtagtaca
     1921 aagccttgtc gaaatgggta tggctcaagt taagcaaatg attatggggc aaactgctgc
     1981 caaggcttct atggcggcgc aaatggtgca agctaaagcg ttagctgctt catttgcacc
     2041 cgcggcctct atggtttctc ttgccacaca gggggcgaat gctattcccg ctcaagctgc
     2101 gatcacgtca actatgagta tggctcaagt cgccgcggtg acaggtcgta aacttggcgg
     2161 ccctgtatca gctagacaga tgtatcgtgt cggcgagaac aatcagcccg aaatcttcaa
     2221 agcgagcaat gggatgcaat atatgatccc cggtcaaagc ggtcgtgtat tcagcaacaa
     2281 gcagtcgcaa ggtcaaggtg gaggcgaaag caaaacagta gctgtcatcg taaatcaaac
     2341 caatcacttt agcagcgatg aagccagtgc aaatcaagcg gctgttgcaa aagcattgag
     2401 caatcaaatc aaggcttccg tgagattgga attgcagtca caaatgcgag ctggtggagt
     2461 tctagcgagg taatcaatgc aaacatttaa tttcaaggtt gagcaagact acactgttca
     2521 aaatgagccc agtgtcgtca tactaaaatt tgccgatggt tacgagcaaa ccgctcccaa
     2581 agggttaaac cacaatctga aaaaatacca aggcattacc gtaaaagcga acaaagcaga
     2641 agcggtgaag attcagcaat ttctcaatgc tcacggtggg tataagaaat tcaactggcg
     2701 cgatagaaca agcgatgaga cggtaacggt tcgttgcaga agctggagct ccactaaatt
     2761 aggtgcagtc attgagttta cacttaattt cgatgaggtg atttaatgcc aaaagccccc
     2821 cccaaaaaaa tggcatcaga gttgataaaa ctcgaacaag atgccctgat tgagctgtgg
     2881 gagatagatc tcactcacat ctactctagc tcaaacccaa atcagcgcgg agagatattc
     2941 cgctttcaca atggcgtgag ccaaacacaa cagaacatct ggtggcaagg caatgaatac
     3001 caagcatacc cgattgccgc cgatggtttt gagatttcgg gtcaaggacc gtcaaatcgt
     3061 ccaacactca ctgtctctaa cctgtacggt gttgtcacgg tattagtaga agatttcggg
     3121 caacccgtag aggcgaaagt cagcagtgaa gagactgtag atggcaatat ccaaatgacg
     3181 gtggaactgc tgaaaaaaat ggatcaaatc gccgatcaac gctaccgtag aaatcagtta
     3241 aatgatttaa gaacaggtgg aacattatct aaataggtga agtatgccaa ttagtatttc
     3301 taaccaaatg aaactagatc ttgccaaact tgagcaaaat gcaatgctgg acttgtacga
     3361 ggttgatttg cgtaaattgc aagacaaaaa cggcaatgct ggtag
//
LOCUS       DANLRK010000211         3394 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.212, whole genome shotgun sequence.
ACCESSION   DANLRK010000211 DANLRK010000000
VERSION     DANLRK010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3394
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.212"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            42..491
                     /locus_tag="PXA33_002385"
     CDS             42..491
                     /locus_tag="PXA33_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014930674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL5121677.1"
                     /translation="MFHFDKQNPHVLFADNRQIETTMKDSGKVRHLEIKPDVLHDFTN
                     MPYENESFWHVVFDPPHLVQGGENSWLVKKYGKLPCNWREVLKKGFDECMRVLKPNGT
                     LIFKWNETQIPVSEILKVIDCQPIYGHKSGRLNQTHWLAFVKIEQSL"
     gene            517..792
                     /locus_tag="PXA33_002386"
     CDS             517..792
                     /locus_tag="PXA33_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HDL5121678.1"
                     /translation="MEQPTEFLTKDELTDLTGYRQKKRQIEWLRERHYIIQDENKYKP
                     LVLYKDVYGHPRQQYPEAQSTGKWKSPAYLMGVKNGKTTQTTESLLT"
     gene            752..1807
                     /locus_tag="PXA33_002387"
     CDS             752..1807
                     /locus_tag="PXA33_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL5121679.1"
                     /translation="MGRPRKQQNRYLPENLRPKTVTRKSGKTVIYWLYRTHGKTEISL
                     GTNRNEAFIKAAQLNIERESQTARITFGLVAKRYRDEVIPQKKASTAQTDLCKIKNLL
                     LFFNDAPLEEITPQHIREYMIWRKDTPGSANNEFSMFSHIWQYAREWGYTNLPSPNIG
                     MKRYPIRKREIYVENNIFQMVYEVADQDMRDLMDLAYITGQRPVDLVNIRKEHIFDGY
                     LHIVQQKTQAKLRIEIVGKLADIIQDRLEKATGTHLFSTKKGEKLTARKLGKRFTYIR
                     EQAVKNNPEYANEIMAFQFRDLRAKSGTDKALSLGEEAARQQLGHTSVQMTKTYIRKA
                     PIVTPIKESVPSSKHQS"
     gene            complement(1934..3007)
                     /locus_tag="PXA33_002388"
     CDS             complement(1934..3007)
                     /locus_tag="PXA33_002388"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HDL5121680.1"
                     /translation="MAQQITLFEDVPLPLTLGGELSPITVAYQTYGRLNAEKSNAVLL
                     CHALTGDAKPYYDSPTEKGWWQDFIGKGLAFDTDKYFFICSNVLGGCKGTTGPASINP
                     KTNKPYGSLFPIITVQDIVNVQKALLDYLEIPQLHAVVGGSFGGMQATQWGISYPDKV
                     RNIINLCSSLTLSAEAIGFNHVMRQAIINDPHFNGGNYYDGIAPDNGLKIARMLGMLT
                     YRTDIQLAKAFGRETKQQGQIWGDHFQVESYLSYQGTKFLGRFDANTYLRLLRALDLY
                     DPALGYENEETALKRIKANYTFVAVTSDQLFKQVDVYKSKQKLEQAGVNLEFHEFHSD
                     FGHDAFLVDYDFFEPMIKAGLAK"
BASE COUNT         1125 a          651 c          673 g          945 t
ORIGIN      
        1 tgcaaacaaa accgatatta gatgcctgtt gtggttcccg tatgtttcat tttgacaagc
       61 aaaatccaca cgttttattt gctgataatc gacaaataga aaccacaatg aaagacagtg
      121 gcaaagtccg ccatttagaa atcaaacctg atgttttaca cgacttcacg aatatgccat
      181 acgaaaacga gagtttttgg cacgttgttt ttgatccgcc acatttagtt caaggtggag
      241 aaaatagctg gcttgtgaag aagtacggca aattaccttg caactggcga gaagtcttga
      301 agaaaggctt tgacgaatgt atgcgagtgc taaaaccaaa cggcacgctt attttcaagt
      361 ggaatgaaac acaaatcccc gtttcagaaa ttttaaaagt tattgattgt cagccgattt
      421 atggacacaa aagcggacgc ttgaaccaaa cacattggct ggcgtttgtg aaaattgagc
      481 aatccctcta aattgagggg gttatttgga gagaatatgg aacaaccgac tgaattttta
      541 acgaaagacg aacttactga tttaacaggc tatcgtcaaa agaaaaggca aatcgaatgg
      601 ttgagggagc gtcactatat aattcaagat gaaaacaagt ataagccatt agtgctatac
      661 aaagatgtct atgggcaccc ccgccaacaa tatccagaag ctcaatccac tgggaaatgg
      721 aaatcacctg cttatttaat gggagtgaaa aatgggaaga ccacgcaaac aacagaatcg
      781 ctacttacct gagaatttac gtccaaaaac agttacacga aaatcaggca aaactgtcat
      841 ttattggcta tatcgcacgc acggaaaaac ggaaatctct ctaggcacaa atagaaatga
      901 ggcttttatt aaggctgctc aactcaatat agaaagagaa agccaaactg ccagaattac
      961 cttcgggctt gtagccaaac gttatcgaga tgaagttata cctcagaaaa aagcgagcac
     1021 agcacaaaca gatttgtgca agataaaaaa tctactgcta ttctttaatg acgctcctct
     1081 cgaagaaatc acccctcaac atatccgaga gtatatgatt tggcgaaaag acacacctgg
     1141 atctgctaat aacgaatttt caatgttcag tcatatttgg caatacgctc gagagtgggg
     1201 ttatacaaac ttaccaagcc ctaatatcgg aatgaaacgc taccctatca gaaagcgtga
     1261 aatttacgtt gagaacaata tatttcagat ggtatatgaa gtggcagatc aggatatgag
     1321 agacttaatg gatctcgcct atatcaccgg acaacgtcct gtcgatttgg tcaatatccg
     1381 gaaagaacac atttttgatg gttatctcca tattgttcag caaaaaacac aagccaaact
     1441 acgtattgag atagtgggaa aactggctga tattatccaa gataggctgg agaaagctac
     1501 tggaacacac ttattcagca ctaagaaagg ggaaaagcta accgctagaa aactaggtaa
     1561 aaggttcact tatattcggg aacaagcggt caagaataac ccggaatacg caaatgaaat
     1621 aatggcattc caatttcggg atctaagagc taaatcagga acagataaag ccctttctct
     1681 aggtgaagaa gccgctcgcc aacaactagg tcatactagc gttcaaatga ccaaaacata
     1741 cattcgaaaa gccccaatag taacccctat caaagaaagt gttccctcct caaaacacca
     1801 gtcttaaaag acagtttcgg aacattttgc attttttcgg aacgtttaca taaaaatcac
     1861 ttataataga ttgaaagata agtaattttt gttactcgtt attactgata atatttttaa
     1921 taaaaaatgc aatttatttt gctaatccgg ctttaatcat cggttcaaaa aagtcataat
     1981 caaccaaaaa ggcatcgtgt ccgaaatcgg agtgaaactc gtggaactcc aaatttacgc
     2041 ctgcctgttc taatttttgt ttgctcttat acacgtcaac ttgcttgaat agttggtcgc
     2101 ttgttaccgc tacaaaagtg tagttggctt taatacgttt tagggcggtt tcttcatttt
     2161 catagccaag agccgggtcg tataaatcta atgctcggag taaacggagg taagtgttgg
     2221 catcgaaacg acctaaaaat ttcgtgcctt gatagcttaa ataggattcc acttggaaat
     2281 ggtcgcccca aatttgtcct tgctgtttgg tttctcgtcc aaaggcttta gcaagttgaa
     2341 tatcagtacg gtaagtcagc ataccgagca tacgggcgat ttttaagccg ttatcaggtg
     2401 caatgccatc ataatagttc ccaccgttaa agtgtggatc gttaataata gcttggcgca
     2461 taacgtggtt aaaaccgata gcctcggcac ttagggtaag tgacgaacaa agatttataa
     2521 tgttgcgaac tttatcaggg taactaatac cccactgtgt tgcttgcatt ccgccgaaag
     2581 atccccctac cactgcgtga agttgaggga tttctaaata gtccaataat gctttttgta
     2641 cattaacaat atcttgcact gtaataattg gaaacaaact accgtaaggt ttattggttt
     2701 tagggttaat cgaagctggg ccggttgtgc ctttacaacc gcctaataca ttactgcaaa
     2761 taaagaaata tttatcggta tcaaacgcaa gccctttgcc gatgaaatct tgccaccagc
     2821 ctttctcggt tggtgagtca tagtaaggct tagcatcacc ggtaagggcg tggcaaagca
     2881 agaccgcatt gcttttctct gcatttaatc tgccgtaagt ctggtaagcc actgtgatag
     2941 gggacagttc gcctcctaat gtgagtggta aaggtacgtc ctcaaaaagg gtaatctgct
     3001 gagccataaa aatcctgttt gcaaaaaatt ctgctttttt gaccgcttgt tgtaatgacc
     3061 gttaaattag cggtgttcat ttcaattgtc aagaattttt agacgtctaa acgtctagat
     3121 ttttaaacgg ctaaaatcaa aattttattg ctgagaagat atttattact taacaaataa
     3181 catgaaatag aataaattta ttgaacgatt agactatttt gtttgtctct aatttaccca
     3241 tcttaaatag ggtagcaaaa gtaaaggatc tcatttgaac caaacgcgtg ataggttaga
     3301 aaaattgcca cttttccata aaatgcagca gtttattatt aaggcattga aaataattgc
     3361 aattttcccc ttattgggaa tagctatatt atta
//
LOCUS       DANLRK010000212         3365 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.213, whole genome shotgun sequence.
ACCESSION   DANLRK010000212 DANLRK010000000
VERSION     DANLRK010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3365
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.213"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            77..865
                     /locus_tag="PXA33_002389"
     CDS             77..865
                     /locus_tag="PXA33_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121681.1"
                     /translation="MFLSHFKQVLNANIEADGKDWTIIDVFGGSGLLAHTAKREKPLA
                     RVIYNDFDNYAERLNHIKETNQLRQEIYQIVDEIIPKNKRISNEIKAKIINKINDFEG
                     FKDLKCLSSWLLFSGEQVATLDELFKHDFWHCVRQSDYPEATGYLDDIEVVSESFHQL
                     LPRFHDKEKVLLILDPPYLCTRQESYKQENYFDLIDFLRLIDLTKPPYIFFSSTKSEF
                     IRFIHYMVQNRKENWQAFEGAERIVVNASASYNGKYEDNMVYKF"
     gene            complement(902..3340)
                     /locus_tag="PXA33_002390"
     CDS             complement(902..3340)
                     /locus_tag="PXA33_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family igA-specific metalloendopeptidase"
                     /protein_id="HDL5121682.1"
                     /translation="MKHTFKLSLLATSIAISLSPTLLASIVRGDVDYQYFRDLAENKG
                     KFFVGATNIPVIDKTGKNIGTFLQVTPTKQVEVESNISPKPNVSLSECFDNESLPGCY
                     EFLYGGGDEDSNDLPVFETVTGETMSIPMIDFSSITRQTGIATLVNPQYVISVNHNQG
                     YRDIQFGDSSSEKADDHHYNYSVVRRNNYMPDPNKERTDLGKGIEKRERNDEGEKLIL
                     DGSGNPTPRWDYHAPRLSKLVTEVAPANEIERTTENVQDFYSVFSDPFIFPMFIRAGS
                     GRQAVVNNERDRLNNGRIEVGNGPFLTGGSVLPVTNGDPSVPGSDFNSRILVAKTENN
                     TINDVFKDHGYGPLTTLGLPGDSGSALFGYDVRTKKWVVLGVYSDYFSENNTLGGDVY
                     KSYWNYHHPHYVRALEKENNAGAINANGARLTWTPSGNTSSIVGGNAPLTVNLADTSL
                     PTLQGHNGNTGDNPWNPQLQHGKTFHILGENNTLSLTENINQGAGAIHFHGNTTVEGM
                     KAGITWLGAGVDIDKDKNVIWKISNPAGDRLSKIGQGTLTINGKGENKGSVSVGDGTV
                     ILNQQADENNKKSAFSELGIVSGRPTVILNSSDQMDPNNIYFGYRGGRLDVNGNDLTF
                     RRIQNSDEGAMVVNHNTAQTANITLTGIVSPTIDQVKVQAITSPDQVSRHLDKDIFTY
                     QRFPHLPTNFIRLKKPLSVAEITAIFNARKVFIDLDFATGLIRENEYFEVLKVTDESA
                     AKQQILAEKIKAAEGTVQGFNGYFGETDPSKTNGRLNITMNSNCSEPTKLRNATTKTT
                     KENRSFTFLILMVF"
BASE COUNT         1000 a          628 c          664 g         1073 t
ORIGIN      
        1 caagcaagca agcaagcatt gtgagataga gaaagtattt aaacaagctc ctttaccctt
       61 ttcagggcaa aaacggatgt ttttatccca tttcaaacag gtgttaaatg caaatattga
      121 agcagacggg aaagattgga ctattataga tgtttttggt gggagtgggt tgcttgcaca
      181 taccgcaaag cgtgagaaac cgttagcaag agtgatttat aatgattttg ataattacgc
      241 cgaacggcta aaccatatca aagagacaaa tcaacttcgc caagaaatct accaaattgt
      301 tgatgaaatt ataccaaaaa ataagagaat tagcaatgaa attaaagcta aaatcatcaa
      361 taaaatcaat gattttgagg ggtttaaaga cctcaaatgc ttaagttctt ggttattgtt
      421 tagcggtgag caagttgcaa ctttagatga gttatttaag cacgattttt ggcactgtgt
      481 gcggcaaagt gactatcctg aagcaacggg ttatcttgat gatattgagg tggttagtga
      541 gtcctttcat caattattgc cgagatttca cgacaaagag aaagtattat tgattcttga
      601 cccaccttat ctttgcacaa gacaggaaag ctataagcag gaaaattact ttgatttaat
      661 tgatttctta cggttgatag accttactaa accaccttat atcttcttta gctcaaccaa
      721 aagcgagttt attcgcttta ttcactatat ggttcaaaac agaaaagaaa actggcaagc
      781 gtttgaggga gcggaaagaa tagtggttaa tgcctcggca agttataacg gcaaatatga
      841 ggataatatg gtgtataagt tctaaaaatt taaacgccct ttgatagtga tttaaagggc
      901 gttaaaaaac cattaaaatt aagaaagtaa agctcctgtt ttctttcgta gttttcgtgg
      961 ttgcgtttcg cagttttgtt ggctcgctac aattactgtt cattgtaatg tttaatcgac
     1021 cattggtttt gctaggatca gtttcaccaa aataaccgtt aaaaccttgg acggtgcctt
     1081 ctgctgcttt gattttttca gcaagtattt gttgctttgc agcactttca tctgttacct
     1141 tgagtacttc aaagtattcg ttttcacgaa tgagaccagt agcaaagtct aaatcgatga
     1201 aaactttacg cgcattaaaa atagctgtga tctctgcaac gcttaaaggt tttttcaaac
     1261 ggataaaatt agttggcaag tgtggaaaac gttgatatgt gaaaatatct ttgtccaaat
     1321 gacgagaaac ctgatctgga ctagtaattg cttgaacttt cacttggtca attgttggac
     1381 taactatgcc ggttagtgta atgtttgctg tttgggctgt attatgattg acaaccatcg
     1441 ctccttcatc gctattctga atacggcgga acgtgagatc attgccgttg acatctaagc
     1501 gcccaccacg ataaccaaaa tagatattgt taggatccat ttgatctgat gaattgagga
     1561 ttacggtagg tctgccactc acaataccta gctctgaaaa tgcagatttt ttgttatttt
     1621 catccgcttg ttgatttaat ataacagtgc cgtcaccaac gctaacggag cctttgtttt
     1681 ctcctttgcc gttgatagtt agtgtccctt gtccgatttt tgataatctg tcaccagcag
     1741 ggttactgat tttccagatc acatttttat ctttatcaat atcaacaccg gctcctaacc
     1801 aagtaatgcc tgctttcata ccttccacag tggtattacc atgaaaatgg attgctcctg
     1861 caccttggtt gatattctct gtgagactta gcgtattgtt ttcacccaaa atatggaatg
     1921 ttttaccgtg ttggagttga ggattccaag ggttatcgcc tgtattacca ttatgacctt
     1981 gaagtgttgg taatgaagta tctgctaaat taactgtaag aggagcattc cctcctacga
     2041 ttgagctggt attgccagaa ggtgtccaag tgagtctagc accatttgca ttaatagcgc
     2101 ctgcattatt ttctttttct aaagcacgta cataatgtgg gtggtgatag ttccagtaag
     2161 atttataaac atctccccca agcgtattat tctctgagaa atagtcacta tatacaccta
     2221 acacaaccca ttttttggta cgaacatcat aaccaaaaag agcggagcca ctatcgccag
     2281 gtaaaccgag agttgttaag ggaccgtagc cgtggtcttt aaatacatcg tttatggtat
     2341 tattttcagt ttttgctact aaaatacggc tgttaaaatc gctgcctgga acgcttggat
     2401 cgccattagt aacaggcaga acgctacctc ctgtcaaaaa aggaccgtta ccgacttcaa
     2461 tacgcccgtt gtttaagcga tctctttcgt tgttaaccac tgcttgtcta ccagaaccgg
     2521 cacgaataaa catggggaaa ataaaagggt cgctaaatac actataaaaa tcttgtacat
     2581 tttcggtagt gcgttcaatc tcattagctg gagcgacttc agttactaat ttcgataacc
     2641 taggtgcgtg gtagtcccag cggggcgttg ggttacctga accatctaaa atcagttttt
     2701 ctccttcgtc attacgttca cgtttttcta tgcctttccc gagatctgtt ctttctttat
     2761 ttggatcagg catatagtta ttacggcgca ccacactata attgtaatga tggtcgtcgg
     2821 ctttttcgct agatgaatca ccaaattgaa tgtcccgata gccttggttg tggtttacac
     2881 taataacgta ttgtggatta actaatgttg caatccctgt ttgacgagta atggaagaga
     2941 agtcaatcat tggaatagac atagtctcac ctgttactgt ttcaaaaaca ggtagatcat
     3001 tagagtcttc atcacctcca ccatagagaa attcataaca accgggtaaa ctttcattat
     3061 caaagcattc agataggctt acattcggct taggtgaaat atttgattcc acttcaacct
     3121 gcttagttgg tgtaacctgt aaaaaagtac cgatgttttt gcccgtttta tcaataactg
     3181 gaatattggt tgccccaaca aaaaatttgc ctttattttc tgctaaatca cgaaaatatt
     3241 gataatcgac atcaccacgt acaatagatg ccagtaatgt tggtgaaaga gaaatagcaa
     3301 ttgatgttgc tagaagagag agcttgaaag tatgcttcat tattatttga aacctgtata
     3361 aatat
//
LOCUS       DANLRK010000213         3364 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.214, whole genome shotgun sequence.
ACCESSION   DANLRK010000213 DANLRK010000000
VERSION     DANLRK010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3364
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.214"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(<1..271)
                     /locus_tag="PXA33_002391"
     CDS             complement(<1..271)
                     /locus_tag="PXA33_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL5121683.1"
                     /translation="MIITMKDIAKEANVSLGTVSNVLNGKSNVSLAKIEKVQAAVEKL
                     GYRKNIQASTLKSGTSNKIAIILPNITELKYACLYENLDLICTQND"
     gene            complement(433..1773)
                     /gene="gdhA"
                     /locus_tag="PXA33_002392"
     CDS             complement(433..1773)
                     /gene="gdhA"
                     /locus_tag="PXA33_002392"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HDL5121684.1"
                     /translation="MSDLNALFQKIKQRDPNQAPFHQAVEEVFGSLAPFLAKNPKYTQ
                     QGLLGRIVEPERVISFRVTWVDDKGQVQVNRGYRVQMNSAIGPYKGGIRFHPTVDLGV
                     LKFLAFEQVFKNALTTLPMGGGKGGSDFDPKGKSDAEVMRFCQAFMSELYRHIGADTD
                     VPAGDIGVGGREIGYMFGQYKKLRNEFTSVLTGKSLTWGGSLIRPEATGYGAVYFAES
                     MLATKGQQIEGKSVVISGSGNVAQYAAEKVIQKGGKVLTVSDSNGYVLFPESGMTEAQ
                     LQALLELKNERRERLSVYATEQGLKYFDGQKPWGVKCDIALPCATQNELDTEDAKELV
                     KNGCICVAEGANMPTTLGGVEVFIGAKILYAPGKAANAGGVATSGLEMSQNAIRLSWS
                     REEVDQRLFNIMKAIHENCVENGTEADGFVNYVNGANIAGFKKVANAMLEQGIV"
     gene            complement(1966..2307)
                     /gene="bamE"
                     /locus_tag="PXA33_002393"
     CDS             complement(1966..2307)
                     /gene="bamE"
                     /locus_tag="PXA33_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HDL5121685.1"
                     /translation="MKMKSLLAAMLLALGVTACSTVEKVVYRIDVPQGNYLEQDKIAQ
                     LKVGMTAEQVEYLLGTPMLKNIFETHRWDYVFIKREGYKDPEQRNLFIYFDAKGLVSK
                     IDDSNANAEQK"
     gene            complement(2374..>3364)
                     /gene="yejK"
                     /locus_tag="PXA33_002394"
     CDS             complement(2374..>3364)
                     /gene="yejK"
                     /locus_tag="PXA33_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HDL5121686.1"
                     /translation="VSEIVLHQLQKTEGENPELNTFLRENLLAISPEVDQMMLQLHQA
                     YQSKAKAYGIFKPESVFAQQLNRLLEQETDFLPFSHSCAKMLAAELIKYPFADGGTFI
                     LCRYTFLATEYLFIALIDSRNSMLVDEQLEIKRTQYLEIAQYDIACRINLTELKLDAT
                     SNRYLTFVKGRVGRKIADFFMDFLSAEEGFNPQLQNQTLLQAVSDYCEQGELSAPQTR
                     EVKKQVFEYCKGQINSGEEIALNELSNALPTLNEIDFAQFTQEQEYGLEENIPPMRNA
                     LKTLTKFSGSGKGVTISFNAELLGERLIWDELNDTLTIKGLPANLRDQLERNK"
BASE COUNT          990 a          731 c          568 g         1075 t
ORIGIN      
        1 aatcattttg cgtacaaatt aaatcaagat tttcatataa acaggcatat ttcagctctg
       61 taatattggg caatataata gcaattttat tacttgtgcc actttttaac gtagaggctt
      121 gaatattttt acgataaccc aacttttcaa cggcagcttg tactttttct attttagcta
      181 agctaacatt actttttccg tttaatacat tcgatactgt tcctaacgaa acatttgctt
      241 cctttgcgat atctttcatc gtaatgatca tatactgccc tttcacatac tgctaaaact
      301 gagattggca tagattttac cataattggc aaatgaaata cgatttcaat ttagcttgtc
      361 tctagctaca acctaaaata aaaaaactgc ctcgatatat cgaggcagtt cttgaataat
      421 agggataata aattaaacaa taccctgttc taacatcgca tttgccactt ttttgaaacc
      481 agcaatattc gcaccgttta catagtttac gaaaccatct gcttcagtac cattttctac
      541 gcagttttcg tgaattgctt tcataatgtt gaataaacgc tgatctactt cttcacgact
      601 ccaagataga cgaattgcat tttggctcat ttctaaacct gaggttgcca caccacctgc
      661 gttagccgct ttacccggag cataaaggat tttcgcaccg ataaagactt caacaccacc
      721 taatgtggtt ggcatatttg caccttctgc cacacaaata cagccgtttt tcactaattc
      781 tttagcatct tcggtatcta attcgttttg ggttgcacaa ggtaaagcga tgtcacattt
      841 cacaccccac ggtttttgac cgtcaaagta ttttaagcct tgttctgttg catacacaga
      901 taagcgttca cggcgttcgt ttttcaactc taataatgct tgtaattgtg cctctgtcat
      961 accggattca gggaataaga cataaccgtt agaatctgaa accgttaata ctttaccgcc
     1021 tttttgaatc actttttcag ctgcatattg tgccacgtta cccgaaccgg aaattaccac
     1081 tgattttcct tcgatctgct gacctttagt cgctaacata gactctgcaa aatacaccgc
     1141 accataacca gttgcttccg gacgaattaa gctaccaccc caagtcagcg atttaccggt
     1201 taatactgaa gtaaattcat tgcgtaattt tttgtattga ccgaacatat agccgatttc
     1261 acgacctccc acgccgatat cgcctgcagg aacgtcagta tcagcaccaa tgtggcgata
     1321 taattcgctc ataaacgctt ggcagaaacg cattacttcg gcatcagatt tgcctttcgg
     1381 gtcaaagtct gaaccacctt taccaccgcc cattggcaat gtcgttaacg catttttgaa
     1441 tacttgctca aaggctaaga attttaatac gcctaaatca acggttgggt ggaaacgaat
     1501 accgcctttg tatggaccga ttgctgagtt catttgtaca cggtaaccac ggtttacttg
     1561 cacttgacct ttatcatcaa cccaagtgac acggaagcta attacacgct caggctcaac
     1621 aatacgccct aataaacctt gctgggtata tttcggattt ttagctaaga aaggggcaag
     1681 gctaccgaac acttcttcaa ccgcttgatg gaaaggggct tggttcggat cacgttgttt
     1741 gattttttgg aaaagggcgt ttaaatcgct catattatac tcctgaataa attgatgatg
     1801 ttgtgtattt aagatgagcg aagtctagca agcaatttta gctttgaaaa gatagattag
     1861 aaaaatttat cattaaaata agaaaaatta agtttaaatg cttatttttt attcaaaaaa
     1921 ataagcccac ttttaaaagt gggcttttcg aaccaacaaa tcgtcctatt tttgttctgc
     1981 attcgcattg ctgtcatcaa tttttgatac taacccttta gcatcaaaat aaataaacag
     2041 attgcgttgt tcaggatctt tataaccttc acgtttaata aacacataat cccaacggtg
     2101 ggtttcaaaa atgttcttta acattggtgt accaagcaaa tattcaactt gttccgctgt
     2161 cataccgact ttaagttgag caattttatc ttgttctaag taattgcctt gcggtacatc
     2221 aatacggtac accactttct caacggttga acaagccgtt acacccagtg caagcaacat
     2281 tgcagctaaa agagatttca ttttcataat tgaccttttg ataatccaaa attcagataa
     2341 cgagataata cctaaactga taaaaatttt ccattatttg ttacgttcca gctggtcacg
     2401 cagatttgcc ggtaatcctt tgattgtcag tgtgtcgttt agttcatccc aaattaaacg
     2461 ctctcctaat aattctgcgt taaagctaat cgttactcct ttgcccgaac cggaaaattt
     2521 cgtcagggtt ttaagggcat ttcgcatagg cggaatattc tcttccaagc cgtattcttg
     2581 ctcttgcgta aattgggcaa aatcgatttc attcagtgtt ggcaatgcgt tggaaagctc
     2641 atttaacgca atttcctctc cactatttat ctgcccttta caatattcaa acacctgctt
     2701 tttcacttct cgagtttgag gggcagataa ctccccttgc tcacaataat cgctcaccgc
     2761 ttgcaacaag gtttgatttt gcaattgtgg attaaaccct tcttccgcac ttaaaaaatc
     2821 cataaagaaa tcggcaattt tacgccctac tctgcctttc acaaaagtta aatagcgatt
     2881 tgacgttgcg tcaagtttca gttcagttaa attaatgcgg caggcaatat catattgtgc
     2941 aatttcgaga tattgcgtgc gtttaatctc taactgctca tcaaccagca ttgaattacg
     3001 gctatcaatc aacgcaataa acaaatattc ggtagctaaa aaagtgtaac gacacaaaat
     3061 aaaagtgcca ccatcggcaa acggatattt aatcagctct gctgcaagca ttttggcaca
     3121 gctatggcta aaaggtaaaa aatcggtttc ttgctccaac aagcggttga gctgttgagc
     3181 gaaaaccgac tccggtttaa atataccgta agcctttgct ttactttgat aggcttgatg
     3241 taattgcaac atcatttgat cgacttcagg ggaaattgcc aataaatttt ctcgcaaaaa
     3301 cgtattaagc tccgggttct cgccctctgt tttttgtaat tgatgtaaca cgatttcaga
     3361 aaca
//
LOCUS       DANLRK010000214         3299 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.215, whole genome shotgun sequence.
ACCESSION   DANLRK010000214 DANLRK010000000
VERSION     DANLRK010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.215"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            132..1454
                     /gene="pepP"
                     /locus_tag="PXA33_002395"
     CDS             132..1454
                     /gene="pepP"
                     /locus_tag="PXA33_002395"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HDL5121687.1"
                     /translation="MDLAYLAKMPQEEFTARRERVLEQMQDNSALLVFTESEKRRNSD
                     SEYLFRPDSYFWYLTGLAEPKSAVLLVKSEGKTESIIFVRKKDPLMETWNGRRLGIEE
                     APKTLQLNAAFDIDDIETVLAKKLENLTACYCALGIQEWGDNILFATFEKMKVNRQKV
                     PTTLIDWQPMLSEMRLIKSEFEIALIQQACHISSMAHIRAMKQARPNRYEWEIEGEIQ
                     HEFSRFGARFPAYNSIVAGGENACILHYNENDQVLKGGDLLLIDAGAEFAYYAGDITR
                     TFPINGKFSEPQKELYELTLTMQKEAINLLVPNSSIKAANDKAVQILTQGLVRLGILN
                     GDVESLIEQKAYRQFYMHGLGHWLGLDVHDVGDYGTERDRPLQIGMVLTVEPGIYIPK
                     DADVPEQYKGIGIRIEDNLLITEYGNKNLSSGCPKEIADVEQLMQSLN"
     gene            1570..1755
                     /locus_tag="PXA33_002396"
     CDS             1570..1755
                     /locus_tag="PXA33_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1508 domain-containing protein"
                     /protein_id="HDL5121688.1"
                     /translation="MYFEIYKDAKEEFRWRLKAGNHEVIATNQAYTTKQGCQKGIEAV
                     KKVTPETEVKDLTKAEA"
     gene            1884..3200
                     /gene="argA"
                     /locus_tag="PXA33_002397"
     CDS             1884..3200
                     /gene="argA"
                     /locus_tag="PXA33_002397"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HDL5121689.1"
                     /translation="MQNTELALAQWFRQSTPYVNMHRGKTFVIMLDGDVIESPNFVNI
                     INDISLLHSLNIKLVIVFGARPQINQLLKQNHIEPEYYHNIRVTNPQSLEYVKQAVGK
                     VHYDFFARLSLRLPHSEVINVVSGNMILAQPIGVVDGVDYTLSGKIRRINIESIKQQL
                     AQNSIVLLGPIAPSVTGEMFNLPFEDIATQVAIKLRADKLIGFCEEQGILDENGKPIP
                     DYLPQDGETLLQQLIERGEYHTPKARFLQAAITVCREGVKRSHLLSYKEDGSLLQELF
                     SRDGIGTQFSMESSEQVRIANSSDIPGLLDLIRPLEQQGILVKRSREQLEMEITNYTI
                     IERDGVVIACAALNPYPEEKMAEMACVAVHPDYRDSSRGDILLENIQKRAVQLGMEKL
                     FVLTTRTTQWFQERGFRLAGAQDLPRYKRENYNYQRRSKVLILPLW"
BASE COUNT         1037 a          576 c          743 g          943 t
ORIGIN      
        1 ttaatctgtt ttgtttttgc aatcaagatc tcaaatataa tctttcatct gttttggtta
       61 ggctgaattt atgctgcgtt tgtgctaaaa taaggcaggt ttcgatatag atataataaa
      121 aaggagaaat aatggatctc gcttatttgg ctaaaatgcc acaagaagaa tttaccgcac
      181 gccgtgagcg tgtgcttgag caaatgcagg ataattctgc gttattggtt tttaccgaat
      241 cggaaaaacg ccgtaatagt gactctgaat acttgtttag acctgatagt tacttttggt
      301 atttaaccgg tttggcagaa cctaaatcag ccgtattgct ggtaaaaagc gaaggtaaaa
      361 cggaaagtat tatttttgtg cgtaaaaaag accctttaat ggaaacctgg aatggcagac
      421 gtttagggat tgaggaagct ccaaaaacgt tacagcttaa cgcagctttt gatattgatg
      481 acattgaaac cgttcttgca aaaaaattag aaaatctgac cgcttgttat tgtgcgttag
      541 ggatacaaga gtggggcgat aatattttgt ttgcaacatt tgaaaaaatg aaagtcaatc
      601 ggcaaaaagt gccgacaacc ttgattgatt ggcagccaat gttatcggaa atgcggttga
      661 ttaaatcaga atttgaaatt gccttaattc agcaggcgtg tcatatttct tcaatggcac
      721 atattcgagc gatgaaacaa gctcgcccga atcgttatga gtgggaaatt gagggggaaa
      781 ttcaacacga atttagccgt tttggagcaa ggtttccggc atataattca atcgttgccg
      841 gcggtgagaa tgcctgtatt cttcactaca atgaaaatga tcaggtgctt aaaggcggcg
      901 atttattact gattgatgca ggggcggaat ttgcgtatta cgccggcgat attacccgaa
      961 ccttccctat taatgggaaa ttctccgaac cgcaaaaaga gctgtacgaa ttaaccttaa
     1021 ctatgcaaaa agaggcgatt aatcttcttg tgccaaacag ctcgattaaa gcggcaaacg
     1081 ataaggctgt acagattctc acgcagggct tggttcgctt aggaatttta aacggtgatg
     1141 tggaaagcct gattgagcaa aaagcctatc gccaatttta tatgcacgga ttagggcatt
     1201 ggctagggct agatgttcac gatgtggggg attacggcac ggagcgggat cgtcctttgc
     1261 agattggtat ggtgttaact gttgaacccg gtatctatat tcctaaagat gcggatgtac
     1321 cggagcaata taaaggtatt gggattcgga ttgaagataa tttgctgatt accgaatacg
     1381 gcaacaaaaa cttaagcagc ggttgcccta aagaaattgc ggatgttgag cagttgatgc
     1441 aatcgttaaa ttaattcgca aatttttgta aaaaaatgac cgcttgcttt gatattttgg
     1501 ggaatttagc ttaagctaac ccgacacaaa cacagtgttt gggcaatcac aacacgaagg
     1561 agtgtttata tgtattttga aatttacaaa gatgcaaaag aagaatttcg ttggcgttta
     1621 aaagcgggta accacgaagt tattgcaacc aaccaagctt atacgactaa gcaaggttgt
     1681 caaaaaggca ttgaagcagt taaaaaagtt acacctgaaa cagaagtaaa agatttaacc
     1741 aaagctgaag cataatctat aaagccctga tattatttca gggctttgtt atttatgcaa
     1801 atataatgct taattattca atttaaggct tgcggatttt tctaaaaaag ataatatgcg
     1861 tttatattat aaagaggaaa tatatgcaaa acacagaact cgcattagct caatggttta
     1921 ggcaatctac accttatgtt aatatgcaca gaggcaaaac ctttgtgatt atgcttgatg
     1981 gcgatgttat cgaaagcccg aattttgtca atattatcaa tgacatcagt ctcctgcaca
     2041 gcctgaatat caaattagtg attgtatttg gggcaagacc gcaaattaac caattgttaa
     2101 aacaaaatca tattgagccg gagtattacc acaatatccg tgtgactaat ccacaatcgc
     2161 tagaatatgt gaaacaagcg gtagggaaag ttcattatga tttctttgcc cgcctttctt
     2221 tgcgtttacc ccattctgaa gtgattaatg tagtcagtgg taatatgatt ttagctcagc
     2281 cgattggcgt ggtggatggg gtagattata cactaagcgg taaaatccgt cgtattaata
     2341 ttgagagcat taagcagcaa ctggcacaaa attcgattgt attacttggt ccgattgccc
     2401 cgtctgttac cggtgaaatg tttaatctgc cgtttgaaga tattgccaca caggttgcaa
     2461 ttaaacttcg tgccgataaa ttaatcggtt tttgtgaaga gcaggggatt ttagatgaaa
     2521 acggcaagcc aattccagat tatcttccgc aagatggcga aaccttgtta cagcagttaa
     2581 tcgagcgtgg cgaatatcac accccgaaag ctcggttttt gcaggcggcg atcacggtat
     2641 gccgagaggg agtgaaacgc tcgcatttat taagctataa agaagatggc tcattgcttc
     2701 aagagttatt ctctcgtgat ggtattggca cacagttctc aatggaaagc tcggaacagg
     2761 tacgtattgc taattccagt gatattccgg gattattgga tttaatccgc ccacttgaac
     2821 agcagggcat tttagtcaaa cgctctcgag aacaattaga aatggaaatc acaaactaca
     2881 ccattattga gcgtgatggt gtggtgattg cttgtgcggc acttaacccg tatccggaag
     2941 aaaaaatggc ggaaatggcg tgtgtggcgg ttcaccctga ttatcgtgat tcctcaagag
     3001 gggatattct actggaaaat attcaaaaac gggcagtgca actgggaatg gagaaactct
     3061 ttgtgctgac tacaagaacc acacaatggt tccaagagcg aggatttagg ctggcaggtg
     3121 cccaagattt acctcgctat aaacgggaaa attacaatta tcaacgtcgt tctaaagtat
     3181 taattttacc actttggtaa aatattcaat gtttttgacc gcttgttacc aagagaacta
     3241 taaaaggaga actatgaaaa aacatcaacc catcaaaggg gctatggcaa ttattactg
//
LOCUS       DANLRK010000215         3271 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.216, whole genome shotgun sequence.
ACCESSION   DANLRK010000215 DANLRK010000000
VERSION     DANLRK010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3271
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.216"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            89..625
                     /gene="proQ"
                     /locus_tag="PXA33_002398"
     CDS             89..625
                     /gene="proQ"
                     /locus_tag="PXA33_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HDL5121690.1"
                     /translation="MSEQIEQKVEQTQNKTNPSTKEVIAYLAEKFPLCFTLEGEVKPL
                     KVGLFQELAEALKDDEKISKTLLRQVLRNYTNSWRYLAASQANVARVGLQGEEVGVVT
                     EDEATHAAEALAAAKAVVAERKAQERKAQRKEYFKQKAREENAKKRAEFKAKKAIDNL
                     PKASEEKLAALADKFGRK"
     gene            711..2723
                     /gene="prc"
                     /locus_tag="PXA33_002399"
     CDS             711..2723
                     /gene="prc"
                     /locus_tag="PXA33_002399"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HDL5121691.1"
                     /translation="MKLTRLNKIFALALGLMIGNAFAVQPDIKESAIVMPKPNEQHSL
                     STKRVTARLTQSHYHKFQLNDEFAGKIFNRYVDWLDPSHNTFLQADIDEMRSKYATTL
                     DDDLYAGKLDAAFAMYELMSKRRYERYSYALSLLDKEPDLKGNDEIEIDREKAAFPKS
                     VEEANTLWEQRVKNDIINLYLKDKKWPEIKKTLTKRYNFAIKRLTQVKADDILQTYLN
                     SFSREIDPHTSYLSPRAAKAFQESMNLSLEGIGATLTQEDDVTSIRALVPGAPAAKSK
                     KLVVGDKIVGVGQGERGEIEDVIGWRLDNVVDKIKGKKGTKVRLEIEPEKGGKTKIIT
                     LVRDKVRIEDSAAKLTVEKVDGKNVGVIKIPTFYIGLTSDVRKLLTQIEKKNVEGLII
                     DLRENGGGSLTEVIELTGLFIKDGPVVQVQDAFNRIKVHEDPESKSVYDGKVMVMINR
                     HSASASEIFAAAMQDYNRAIIVGQQTFGKGTVQQSRSLNFVYDLDQEPLGFIQYTIQK
                     FYRINGGSTQLKGVEADIKFPEIINAAKTGESFEDNALPWDKIPAANYKEAGNARNAV
                     AALTAKHQERIAKNPEFIVLNENIAIRKERDERKFLSLNLEERLKENKADESKRLKDI
                     NARFAREGKKALKDIDDLPKDYEAPDFFLKEAEQMMLDWSEFDKKS"
     gene            2814..>3271
                     /locus_tag="PXA33_002400"
     CDS             2814..>3271
                     /locus_tag="PXA33_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HDL5121692.1"
                     /translation="MKTFKLLPIMVAFSGMALAETATVAQVASEPVVQDNILPQLSFA
                     AQQARAAELVKQADYALEQEQIRQEQARKFAEAEARIKQEIGNNALLLSQATTKVYLD
                     SEFATMWNDKAAEKAFLKEYALFAVSGVSSKSAKALQQILNQPEGLGRDI"
BASE COUNT         1136 a          509 c          717 g          909 t
ORIGIN      
        1 atatggacga agccttataa atcaaatata gtatccagtt agattttcac ttatctaatt
       61 attagatttt tattaaatag gacttattat gtcagagcaa attgagcaaa aagtagaaca
      121 aactcagaat aaaaccaatc caagtaccaa ggaagtgatt gcttatttag cggagaaatt
      181 tccgctttgt ttcacgcttg aaggtgaagt aaaaccattg aaagttgggt tgtttcagga
      241 attagctgaa gcattaaaag atgatgagaa aatcagtaaa acgttattgc gccaagtatt
      301 gcggaattac actaattctt ggcgttactt ggctgccagc caagctaatg ttgctcgtgt
      361 cggcttacag ggtgaagaag ttggcgtggt aacagaagac gaagctactc acgccgcaga
      421 agctttggct gctgcgaaag cggtggttgc agaacgtaaa gcacaagagc gtaaagcaca
      481 acgtaaagag tacttcaaac agaaagcacg tgaagaaaat gcgaaaaagc gtgcagaatt
      541 taaggcgaaa aaagcaatag ataatttacc taaagcttca gaagaaaaat tagctgcatt
      601 agcagataaa ttcggtcgta aatagttttc aagtcgcttt gtgcttacaa gcggtcggat
      661 tttagtaaaa ttttgcaaaa aaatgaccgc ttgattattt ggaaaagaag atgaaattaa
      721 caagattaaa taaaattttt gccctagccc taggtttaat gataggaaac gcttttgccg
      781 ttcagccgga cattaaagaa agtgcgattg tgatgccaaa acctaatgag cagcatagct
      841 tatcaaccaa gcgtgtaaca gcccgattaa ctcaatcgca ttatcataaa tttcaactga
      901 atgatgagtt tgcagggaag atttttaacc gctatgtcga ttggcttgat ccttctcata
      961 atacttttct tcaagccgat attgatgaaa tgcgtagtaa atatgctaca acattagatg
     1021 atgatttata tgctggtaaa ttagatgcag catttgcaat gtatgagtta atgtctaaac
     1081 gtcgttatga gcgttatagc tatgccttat ctttactaga taaagagcct gacttaaaag
     1141 ggaatgatga aattgagatt gaccgtgaga aggccgcatt tccaaaaagt gtagaagagg
     1201 caaatacact ttgggagcag cgagttaaaa acgatattat caatttatat cttaaagata
     1261 aaaaatggcc tgaaattaag aaaacactca ctaaacgtta taactttgcg attaagcgtt
     1321 taactcaagt taaggctgat gatattttac aaacttactt aaattcattc tctcgtgaga
     1381 tcgatcccca cactagctat ttgtctcctc gtgcggcaaa agcgttccaa gagagcatga
     1441 atctgtcgct agaaggaatt ggtgcaacgc taacccaaga agatgatgta accagcatta
     1501 gagcattagt gccaggagcc cctgccgcta aaagtaagaa gctggtagtc ggcgataaaa
     1561 ttgtcggtgt aggtcaaggt gagagaggtg agattgaaga tgttatcggt tggcgtttgg
     1621 ataatgtagt cgataaaatc aaaggtaaaa aaggcactaa agttcgttta gaaattgagc
     1681 ctgaaaaagg tggaaaaact aaaattatta cccttgtgcg tgataaagtt cgtattgagg
     1741 atagtgctgc taaattaacg gtagaaaaag tagatggaaa aaatgtaggg gtaattaaga
     1801 tccctacttt ttatatcggt ttgacttcag atgtacgtaa attacttact caaatagaga
     1861 aaaaaaatgt tgaaggattg atcattgatt tacgtgaaaa cggtggtggt tcgttaactg
     1921 aagtgattga attaactggt ttatttatta aagatgggcc tgtagtgcaa gtacaagacg
     1981 cattcaatcg tatcaaagta cacgaagatc cagaatcaaa atcagtttat gatggaaaag
     2041 tgatggtgat gattaatcgc catagtgctt cagcctctga aatcttcgct gcggcaatgc
     2101 aagattacaa ccgtgcaatt attgtagggc aacaaacctt tggtaaggga actgttcaac
     2161 agagccgttc attgaatttt gtatatgatt tggatcaaga gcctcttggt tttatccaat
     2221 atacgattca aaaattctac cgtatcaatg gtggaagtac ccagctaaaa ggggttgagg
     2281 cggatattaa attcccggaa atcattaatg ctgcaaaaac aggtgaaagt tttgaagata
     2341 atgcgcttcc ttgggataaa attccagcag ctaattataa agaggctggg aatgctcgta
     2401 acgcggttgc tgcattaaca gcgaaacatc aagagcgtat tgctaaaaac ccagagttta
     2461 tagtattaaa cgagaatatt gcgattcgaa aagaacgtga tgagcgtaag tttttatcgt
     2521 taaatttaga agaacgcctg aaagaaaata aagcagatga aagcaagcgc ttaaaagata
     2581 ttaatgctcg ttttgcccgt gaaggcaaaa aagcattaaa agatattgat gatttaccaa
     2641 aagactatga agcaccggac tttttcttaa aagaagctga acagatgatg cttgattggt
     2701 cagaatttga taaaaaatca taaatgtgtt tataatgcgc acataacgta ccgcttagct
     2761 ccgctaagcg gtttattttt ttataaattt gcaaatatta agaggaagag tgggtgaaaa
     2821 cctttaaatt attacctatt atggtggcgt tttctggcat ggctttggct gaaacagcca
     2881 cagttgctca ggtggcttct gagcctgttg tacaagataa tattttacca caattaagtt
     2941 ttgcagctca gcaggctcgt gctgcagaat tggtaaaaca ggccgattat gcgttagaac
     3001 aggaacaaat tcgtcaagag caagcaagaa aatttgcaga agcggaagca agaattaagc
     3061 aagaaatagg taataatgcc ttattgctga gccaagcaac aactaaggtt tatttagaca
     3121 gtgaatttgc gacaatgtgg aatgataaag ccgcagaaaa agcatttttg aaagaatatg
     3181 cattatttgc agtaagtggg gtatcctcta aatcagctaa ggcattgcag caaatcttaa
     3241 atcaaccgga aggtttaggt agagatattt t
//
LOCUS       DANLRK010000216         3264 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.217, whole genome shotgun sequence.
ACCESSION   DANLRK010000216 DANLRK010000000
VERSION     DANLRK010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.217"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            <1..521
                     /locus_tag="PXA33_002401"
     CDS             <1..521
                     /locus_tag="PXA33_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PcfJ domain-containing protein"
                     /protein_id="HDL5121693.1"
                     /translation="FQDIEDYMIHEGYLHGIPQTWKHLVSHSEQWHQHILEEQRRDQH
                     ILEEQRRENDEAHQALLSTSWSSPIDICYINEIEFQPLTTGQMLFDEGQRMRHCIFTY
                     IDICSEGRYLAFSVKIANESATLGLIKHRGNWKIEQFRYACNIAVCEPMILAAAQKLC
                     KLLNKQSALPQN"
     gene            617..1249
                     /locus_tag="PXA33_002402"
     CDS             617..1249
                     /locus_tag="PXA33_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121694.1"
                     /translation="MKTKISLIAVFMLGLSGCVHQQNTAQNSDEITPMPDSLLKPQMQ
                     PLPPQDRIVSKDIYHEQTTSNTEVVRDGRYTLISIAPEEGQKYLLEQVVSVKVPNTKN
                     RFTANVQQGLRTTLTNTGLELCFPPIGEQGNAVQTLFTRPLPKVHYQFGPMKLYEALQ
                     MIAGPAYELTLNDITRTICFKARESSFQNPHKPAVAIQRQQLKPRSLRNN"
     gene            1261..1998
                     /locus_tag="PXA33_002403"
     CDS             1261..1998
                     /locus_tag="PXA33_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03759 family integrating conjugative element
                     protein"
                     /protein_id="HDL5121695.1"
                     /translation="MKRNTINIWLAVLSSVTTNALAQTTSIESSTQTLSQQQSQHQQS
                     QLSDSLKQAKEWGLTIEEWNRYTELMKGERGMWSPNLDPLTALGIEARTAEEREKYAR
                     MLAKKYYERVSKELDFDKVYRQEFEKLYPNELPFEVEPHISQSIGRVIYFTRFDNCDS
                     CKSDLSKILSYANSQTPLDIYIVGQNISDNDIRKWAAENNIDPIKVSKKLITLNHDKG
                     YWFQYSLGKMPAAYQIKQDGEWQSLVY"
     gene            1977..2741
                     /locus_tag="PXA33_002404"
     CDS             1977..2741
                     /locus_tag="PXA33_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HDL5121696.1"
                     /translation="MAISRLLIVITLFAVGISDAKSKPFTGFGTEIDSYIRIAAQRYQ
                     ISEPMLRGLIKMEDGWYGNISPTGATGVGQFTVGTWNWLSETERGKQLGMRLITGKNK
                     GTYADPRRDKYTNTLATALLARWHIEQFHLRRIPATDENLYMAHNIGLDSLHRAMMGK
                     ATKEDILNMRRNGMKSWMTVRDFLNYQKGRFLAHKQEANFMLVSKQGHIHTGVSAFVY
                     PTSEMGKSVKWVEPDNKIIWVEPKSAMIWVEPTNTP"
     gene            2750..3256
                     /locus_tag="PXA33_002405"
     CDS             2750..3256
                     /locus_tag="PXA33_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HDL5121697.1"
                     /translation="MQHKIKMLLLSLCISHSVWGDLTVIADFGGESAVRFYEALQPEH
                     DENAPNYPNSIPASVSEADILPVVSHKLSPGIIETKQFDLTGIQAIFLIGADNTSAQW
                     LQQNREKLITLNATGLVVNVRTLAELNQLREIVPEIIMLPIPGDDLAERIGIAHYPAL
                     ITETGVSQ"
BASE COUNT         1116 a          611 c          634 g          903 t
ORIGIN      
        1 tttttcaaga tattgaagat tacatgatac atgagggata tttacatggt atacctcaga
       61 cttggaaaca tttggtttca catagcgaac aatggcatca acacatatta gaagagcaac
      121 gaagagatca acacatatta gaagagcaac gaagagaaaa tgatgaggct caccaagcac
      181 ttttatctac atcttggtca tctccaattg atatttgcta tatcaatgaa attgaatttc
      241 aacctttgac gacgggacaa atgttatttg atgaaggaca acgtatgagg cattgtattt
      301 ttacatacat agatatctgc tcagagggga gatacttagc tttctccgta aaaatagcca
      361 atgaatcagc tactttagga ttaataaaac atagaggaaa ctggaaaatt gaacaatttc
      421 gttatgcctg taatattgca gtttgtgagc ctatgatttt agcagcagct caaaaactat
      481 gtaaattact taacaaacaa tcagccttac ctcaaaatta attttatctc ccaatcaaca
      541 atagaataaa tcattcaaaa tcagcaatga cttcgccata gttggcttag attcctttca
      601 cacttggaga gattaaatga aaacaaaaat atccttaatt gctgttttta tgcttggttt
      661 aagtggttgt gttcatcaac aaaatactgc tcaaaacagt gatgaaatca cacctatgcc
      721 tgattcatta ctaaaacctc aaatgcaacc attacctcca caagaccgta tcgtgtccaa
      781 agatatctac cacgaacaaa ctacctcgaa taccgaagta gtacgtgacg gacgttacac
      841 cttaatcagt attgctcctg aggaaggaca aaaatatctg cttgaacaag tggtatcggt
      901 aaaagtgcct aatactaaaa atagattcac agccaatgtt cagcaaggcc tccgtacaac
      961 attaaccaat acagggttag aactgtgctt tccaccaata ggtgaacagg gcaatgcagt
     1021 tcaaacgctt tttactcgcc cacttccgaa agtccattac caattcggtc cgatgaaatt
     1081 atatgaagct ctacaaatga tcgcgggacc ggcttatgaa ttaacactta atgatattac
     1141 tcgtacgatt tgctttaaag ctcgggaaag ctcattccaa aatcctcata agcctgctgt
     1201 tgctattcaa cggcaacaat taaaaccgag gtcattgagg aataattgag ggaaactcca
     1261 atgaaacgaa atactatcaa tatatggtta gccgtactct cttcagtgac tacaaatgct
     1321 cttgctcaaa ctacatcaat agaaagtagc acacagacct tatcgcaaca gcaaagccaa
     1381 catcaacaat cacaattaag tgatagccta aaacaggcaa aagagtgggg attaaccatt
     1441 gaagaatgga atcgctacac cgaattaatg aaaggtgagc gtggtatgtg gtcgccaaac
     1501 ttagatcctc ttacagcatt aggtattgaa gcaagaactg ctgaggagag agaaaaatat
     1561 gctcgtatgt tagcaaaaaa atactatgaa cgtgtgtcca aagaactgga ttttgacaaa
     1621 gtctatcgtc aggagtttga gaaactgtat ccaaatgaac ttccttttga agtagaacca
     1681 cacatttcac aatccatagg tcgagtgatt tattttacac gttttgataa ttgcgattct
     1741 tgtaaaagtg atctcagtaa aatcctctct tatgccaata gccaaacacc gcttgatatc
     1801 tatattgtag ggcaaaacat ttctgataat gatatccgca aatgggctgc tgagaataat
     1861 attgatccga ttaaagtgag taagaaactg attacactta atcatgataa gggttattgg
     1921 ttccaatatt cattaggtaa aatgccggca gcgtatcaaa taaaacagga tggtgaatgg
     1981 caatctctcg tttattaatc gttatcacgc tatttgcggt cgggataagt gatgctaagt
     2041 caaaaccttt taccggattt ggtacagaaa ttgatagcta tatccgtata gcagctcagc
     2101 gttatcaaat tagcgaaccg atgctaagag ggctaataaa aatggaagat ggttggtatg
     2161 gcaatatctc acctactggt gccacaggtg tagggcaatt tactgtggga acgtggaatt
     2221 ggctatctga aacagaacgt ggtaagcagc ttggtatgcg tttaattacc ggtaagaata
     2281 aaggtactta tgctgatcct cgcagagata aatacaccaa tacgttagca acagcgttat
     2341 tagctcgatg gcatattgaa caattccatt tacgtcgcat tccggcaaca gatgaaaatt
     2401 tatatatggc tcataacatt ggtttagata gtctgcatcg agcaatgatg ggaaaagcta
     2461 ccaaagaaga tatcttaaat atgcgtcgca atggaatgaa aagttggatg acagtaagag
     2521 atttcctcaa ttatcaaaaa ggacgctttt tagcacataa acaagaagcc aattttatgt
     2581 tggtctcaaa acaggggcat attcatactg gtgtttccgc ctttgtttat cctacaagtg
     2641 aaatgggaaa atcagtaaag tgggttgagc cagacaacaa aataatttgg gttgaaccta
     2701 aaagtgcaat gatttgggta gaacctacaa atacgccata gaggtaaaaa tgcaacataa
     2761 gataaaaatg ctattgctca gtttatgtat atctcattcc gtttggggcg atttaacggt
     2821 aatcgctgat ttcggcggtg aaagtgcggt acgtttttac gaagctttac aaccggaaca
     2881 cgatgagaat gcaccgaact atccgaattc tattccagct tcggttagcg aagctgatat
     2941 tctacctgta gtctctcata aactgtctcc tggtattatt gaaacaaaac aatttgattt
     3001 aacaggaata caagcgattt ttttaattgg agcagataat acctcagctc aatggttaca
     3061 gcagaatcga gaaaagttga tcacgttaaa tgccactggg cttgttgtga atgtccgaac
     3121 attagcagag ctgaatcaat tacgagaaat tgtgccagaa ataataatgc taccaatccc
     3181 cggagacgac cttgcagaac gaattggtat cgcacattac cctgccctga ttactgaaac
     3241 cggagtttca caataatgtc agct
//
LOCUS       DANLRK010000217         3238 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.218, whole genome shotgun sequence.
ACCESSION   DANLRK010000217 DANLRK010000000
VERSION     DANLRK010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3238
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.218"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(246..1841)
                     /locus_tag="PXA33_002406"
     CDS             complement(246..1841)
                     /locus_tag="PXA33_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL5121698.1"
                     /translation="MRLSQLSLSVLLLSASSLVAAAPVNTLINCVATPPMKLSPAITN
                     DANDFNASSQQIYNRLLAFKAGELTVEPSLAERWEISDDGLSYTFHLRKDVKFHSNKQ
                     FTPTRTLNADDVVFSFQRQMDLNHPYHKVSDGTYFYYHWMNLPKILKTVEKVDDYTVK
                     ITLQQPNAPFLTTLAMDFLSIYSKEYADKLLAEGKPELLDQAPIGTGPFVFQAYQTDQ
                     AVRYVANPDYFQGKAKFEKLIFSITPDAGTRYAKLKAGECDVIDFPNISDIAQMKQDP
                     KVNLLQRDGLNLAYIGLNSTKPALNNAKVRQALHYATDKKAIVEAVYQGGGTVASNPF
                     PDSVLGYNPNLPQYEFNLEKAKALLTEAGFADGFETEIWVQPVVRPSNPNPRRTAEII
                     QADWAKIGVKAKLVTHEWADFNKRTREGEFSTGTYGWTSRNGDPDNFLFPLLSKSNIP
                     GTNYGRWTDEKFEQLLAQAVQTQNRDERAKLYQQAVEIANEQTPFIPLAHAINYVPLS
                     KRVQGFVQNPFGYTPFYGVSLSE"
     gene            1990..2430
                     /gene="mioC"
                     /locus_tag="PXA33_002407"
     CDS             1990..2430
                     /gene="mioC"
                     /locus_tag="PXA33_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HDL5121699.1"
                     /translation="MTSICIITGSTLGGAEYVADHINEVLSEQGFEVELFNQATLADI
                     EGKTNLIIVTSTHGAGELPENIQQLFDDLANSSADFSSMKFGVIGLGSSDYDTFCNAV
                     NIVEEALKAKGASQVCESLRIDVMNNFDHDGSAEEWLPSFVEQL"
     gene            2442..2609
                     /locus_tag="PXA33_002408"
     CDS             2442..2609
                     /locus_tag="PXA33_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HDL5121700.1"
                     /translation="MNKISANKAKWIKIAIILIYMFSPVDILPEAILGPFGLVDDAAA
                     LMLLIKTILEK"
     gene            complement(2698..3171)
                     /gene="asnC"
                     /locus_tag="PXA33_002409"
     CDS             complement(2698..3171)
                     /gene="asnC"
                     /locus_tag="PXA33_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HDL5121701.1"
                     /translation="MYSKNTTKSEIDKLDQKILRWLIKDARTPYAEMAKVFGVSAATI
                     HVRVEKMRQAGIIESTKIVVDERKVGYDVCCFIGIILKSAKDYDKVIAKLSEFNEVVE
                     AYYTTGNYSIFIKVMTHTIEELHSVLAGKIQSIDEIQSTETLISLQNPISRDILP"
BASE COUNT          973 a          662 c          624 g          979 t
ORIGIN      
        1 caatttaaac aagctccgct accttttaca ggtcaaaagc ggatgttttt aaaacacttt
       61 taatgatgat ttaaagggcg tttgttttca gtttattttg cataactttt gtgattacgg
      121 ctatgcaaaa tgatttgcaa aactatgcaa aataaatcga ccgcttacaa ttctaaaaga
      181 aaagcagtct attctctacc acattttgca aaaaagtggc aaaaatagac cgcttgtatg
      241 gcatatcact ccgataaact caccccataa aatggcgtgt agccaaacgg attttgcaca
      301 aaaccttgca cccgtttgct gagtggtaca taattgatag cgtgagccag cggaataaat
      361 ggcgtttgtt cgttggcaat ttccaccgct tgttgataga gcttcgcccg ctcgtcacgg
      421 ttttgggttt gtacagcttg agctaataac tgctcaaatt tctcatccgt ccaacgccca
      481 taatttgttc ccggaatatt agatttgctt aataaaggga agaggaaatt atcaggatca
      541 ccattacggc ttgtccaacc ataagtgccg gtagagaact cgccttcacg ggtgcgtttg
      601 ttaaaatccg cccattcgtg ggtcactaat ttcgctttca ccccgatttt cgcccaatcc
      661 gcctgaataa tttcagccgt tcgacgtggg tttgggttag aagggcgcac taccggttgt
      721 acccaaattt ccgtttcaaa tccatcggca aagccggctt ctgttaatag ggctttggct
      781 ttttcaaggt taaattcata ttgcggtaaa tttggattat agcccaacac cgaatcaggg
      841 aacggattac ttgccaccgt gccaccgcct tgataaacag cctcgacaat cgctttctta
      901 tcggttgcat aatgcaaggc ttggcgaact ttggcgttat ttaatgccgg tttggtgctg
      961 tttaagccga tataagctaa attcagcccg tctctctgta acaaattcac cttcggatct
     1021 tgtttcattt gggcaatatc ggaaatattc gggaaatcaa tcacatcgca ttcacccgct
     1081 ttcagtttgg cataacgagt gcccgcatca ggggtaatac taaaaatcag tttctcaaat
     1141 tttgctttac cttggaaata atccgggttc gcaacataac gcaccgcttg atcggtttga
     1201 taggcttgga acacaaaagg ccctgtgccg atcggggctt gatccaataa ctccggcttg
     1261 ccttccgcca gcaatttatc cgcatattct tttgaataaa tcgacaagaa atccatcgct
     1321 aaggttgtca gaaaaggagc attaggttgc tgtaaggtga ttttaacagt gtaatcgtcc
     1381 actttttcca ctgtttttaa aatcttcggc aaattcatcc aatggtaata gaaataagta
     1441 ccgtcagaga ccttatggta agggtggttt aaatccatct ggcgttggaa cgagaacact
     1501 acatcatccg cattcagcgt tcgagttggc gtaaattgtt tattggaatg aaatttcacg
     1561 tctttacgta aatggaatgt gtaacttaag ccatcgtcac tgatttccca acgttccgcc
     1621 aaactcggct caaccgttag ttcaccggct ttaaacgcta ataagcgatt ataaatttgt
     1681 tgtgaactgg cgttgaaatc attggcatca ttagtaattg caggactaag tttcatcggt
     1741 ggagttgcca cgcaattaat caacgtattt accggagctg ctgccaccaa actgctggca
     1801 cttaagagta aaacagaaag agataattga gaaagacgca taaagactcc ttaacatttt
     1861 ctactaactt actcggcatt aacgtatttt caaaataact aaaggttata atttataatc
     1921 agaataatca gttgcaaacc aatgattctt ggctagaata ttggcagttt tttaaacgag
     1981 agatttacta tgacatcaat ttgtatcatc accggcagca ccttaggcgg tgcggaatat
     2041 gttgccgatc acattaatga ggtattaagc gaacaaggtt ttgaagtgga actgtttaac
     2101 caagccaccc ttgccgatat tgagggcaaa accaatctga ttatcgttac ctctacccac
     2161 ggagccggcg agttacccga aaatatccaa cagctatttg acgatttagc aaatagctcg
     2221 gctgatttca gcagtatgaa atttggtgta atcggtttag gtagctccga ctatgacacc
     2281 ttctgcaatg cagtaaatat tgttgaagaa gctctcaaag cgaaaggggc aagccaagtt
     2341 tgcgaatcac tgagaattga tgtaatgaat aatttcgacc acgatggcag tgctgaagaa
     2401 tggttaccat catttgttga acaattataa cttaagtatc aatgaacaaa atcagtgcaa
     2461 ataaggcaaa gtggattaaa atcgctatta tcttaatcta tatgttcagc cctgtagata
     2521 ttctacccga agcgatttta ggcccattcg gtttggttga tgatgccgcc gcattaatgc
     2581 tattgattaa gactattttg gaaaaatagg catagtagac aaaatagttg caaaaaacca
     2641 aaacaaaacg accgcttgta acccaataca agtggtctta tcttttactt aattctttta
     2701 agggagaata tctcttgaaa taggattttg aagtgaaata agcgtttctg ttgactgaat
     2761 ttcatcaata gactgaattt ttcctgctaa taccgaatgc aactcttcga tggtatgggt
     2821 cattacttta ataaaaatag aataattacc agtggtatag tacgcctcaa caacttcatt
     2881 aaattcacta agtttagcaa ttactttatc ataatctttg gctgatttta aaataatgcc
     2941 aataaagcaa cacacgtcat acccaacctt acgctcgtcc actacgattt tggtagattc
     3001 aataatgccg gcttggcgca ttttttcaac acggacatgg atagttgcgg cactcacacc
     3061 aaaaactttt gccatttccg cataaggcgt gcgagcatct ttaattagcc aacgtaaaat
     3121 tttttgatcc agcttatcaa tttccgattt agtcgtgttt ttactgtaca ttttttctcc
     3181 tcaaaatcaa actaattaga taagatttaa aaatattgca aatattttaa atgaaatt
//
LOCUS       DANLRK010000218         3238 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.219, whole genome shotgun sequence.
ACCESSION   DANLRK010000218 DANLRK010000000
VERSION     DANLRK010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3238
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.219"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(<1..595)
                     /locus_tag="PXA33_002410"
     CDS             complement(<1..595)
                     /locus_tag="PXA33_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL5121702.1"
                     /translation="MQERITQQNERLSAYMDGQNVDTAFVETLTNSPELQQKWASYHT
                     IRSVMQGDEIILGRDFSAKMEALLENEEIESQANVEKPKGLLLKLKRWGTPIMQAGIA
                     ASVCLVAVLGVNSMNASDEIAQTQQPVLQTLPFSNSVEAVSYNAPVQDQPTEEQLELQ
                     QRKINALLENHELQRRTNNVKNLTLSEEEKQKAQTSSN"
     gene            complement(641..1213)
                     /gene="rpoE"
                     /locus_tag="PXA33_002411"
     CDS             complement(641..1213)
                     /gene="rpoE"
                     /locus_tag="PXA33_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HDL5121703.1"
                     /translation="MSEQITDQELVEKAQKGDKKAFNLLVVRYQNRVAGLLTRYVARD
                     DIPDIVQESFIKAYRSLESFRGESAFYTWLYRIAVNTAKNHLTSLGRRPPKEDILAED
                     AESYDVGAQLRESDTPENLILSEELKKVVFDTIENLPDELKTAIRLRELEGLSYEEIA
                     EVMECPVGTVRSRIFRAREAIDAKIKPLMQ"
     gene            complement(1435..2979)
                     /gene="leuA"
                     /locus_tag="PXA33_002412"
     CDS             complement(1435..2979)
                     /gene="leuA"
                     /locus_tag="PXA33_002412"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HDL5121704.1"
                     /translation="MSNNNIIIFDTTLRDGEQSLKASLTVKEKLQIALALERLGVDVM
                     EVGFPVSSAGDFESVQTIARHIKNSRVAALSRAVAKDIDAAYEALKVAEAFRIHTFIA
                     SSALHVEAKLKRSFDDVVEMAVAAVKRARNYTDDVEFSCEDAGRTGIDNICRIVEAAI
                     NAGATTVNIPDTVGFCLPNEYGNIIHQVMNRVPNIDKAIVSVHCHNDLGMATANSLTA
                     VQNGARQIECTINGIGERAGNTALEEVVMAIKTRQEFMGVDTRINTQEIHRVSQMVSQ
                     LCNMPIQPNKAIVGSNAFAHSSGIHQDGMLKNKNTYEIMSPETIGLKKEKLNLTARSG
                     RAAVKGHMADMGYTETDYDLDKLYEAFLQLADKKGQVFDYDLEALAFIDMQQGDEDRL
                     KLDVITTQLISGLPASAFVQVELDGVRQSATSQGGNGPVEATYNAIMNIVGMELKMSH
                     YNLTAKGEGAEALGQVDIVVEYEGRKFHGVGLATDIVESSARALIHAINAIYRSQKVA
                     DLKAKK"
BASE COUNT          879 a          712 c          578 g         1069 t
ORIGIN      
        1 tattggaaga agtttgtgct ttttgctttt cttcctcaga taaggttaaa tttttaacgt
       61 tattagtacg gcgttgtaac tcatggtttt ctaataacgc attaatctta cgctgttgaa
      121 gctctaactg ttcttctgtt ggttgatctt gaactggcgc attataactt accgcttcaa
      181 ctgaattgct aaatggcaat gtttgtaata ctggttgttg cgtttgagct atttcatcac
      241 tcgcattcat tgaattaacc cctaataccg caactaagca cactgaagct gcaataccgg
      301 cttgcataat tggagttccc caacgcttga gcttgagcaa taagccttta ggtttctcta
      361 catttgcttg agattcaatt tcttcatttt ccagtaatgc ttccatttta gcagagaaat
      421 ctctccctaa aatgatctca tcaccttgca tcacactacg aatagtgtga taacttgccc
      481 atttttgctg taattcaggg ctgttagtta atgtttctac aaatgctgta tcaacatttt
      541 gcccatccat ataagctgaa aggcgttcat tttgttgagt gatgcgttct tgcataattg
      601 actccaatga tttaaatctc ttctaaaata atctttctat ctattgcata agtggcttga
      661 ttttagcatc aatcgcttcc cttgctcgga aaatacggga acgtacagta ccaaccggac
      721 attccatcac ttctgcgatt tcttcgtaac ttagcccttc caattctctt aaacgaatgg
      781 cggttttaag ctcatcaggt aaattttcaa tcgtatcaaa cacgactttc tttaattcct
      841 cggataatat caaattctct ggcgtatcac tttcacgaag ttgcgcgcct acatcataac
      901 tttcagcgtc ttcggcaaga atatcttctt ttggcggtct tctgcctaaa gaggttaaat
      961 gatttttcgc agtattcact gcaatgcgat aaagccacgt ataaaaagca ctttcgccac
     1021 ggaaagactc taaagaacga taggctttaa taaaagattc ttgcacaatg tccggaatat
     1081 catctcttgc aacataacga gtcagcaaac cggctactct attttgataa cgtacaacaa
     1141 gtaaattaaa tgctttttta tcaccttttt gtgctttttc gactaattct tggtcggtga
     1201 tctgttcgct caactgcgga ctctcctaaa tatcaatatg tcattttcca ttgcacactt
     1261 tgttagtgtg tgctttatga caaaagttca aaaaatttga aaaattgtgt aattttttac
     1321 attcctagat aaaacctact taataaacta agcaagcggt cggattttag cgaaattttg
     1381 caaaaatttc aaaaaatccg accgcttgtc tatgttatgc tttcgctcta taagttattt
     1441 cttcgctttt aaatctgcca ctttttgtga acggtaaatc gcattaatgg cgtgaattaa
     1501 agctcgggct gaagattcca caatatcggt tgccaaaccc acaccgtgga attttcgacc
     1561 ttcatattcc actacaatat ccacttgccc taatgcttct gctccttcgc ctttcgcagt
     1621 cagattatag tgcgacattt ttagctccat tcccacaatg ttcataatgg cattgtaagt
     1681 tgcctcaact ggaccatttc ccccttgaga tgtagcactt tgacgaacgc cgtctaattc
     1741 cacctgtaca aaagccgaag ccggtaaacc gctgattagt tgggtggtaa ttacatctaa
     1801 ttttaagcgg tcttcatcgc cttgctgcat atcaatgaag gcaagagctt ctaagtcata
     1861 atcaaacact tgcccttttt tatcagcaag ttgcaagaag gcctcataaa gtttatctaa
     1921 atcgtaatcc gtttccgtat aacccatatc agccatatgc cctttcaccg ctgctctacc
     1981 tgaacgggca gttaaattca gcttctcttt tttcaagccg atggtttccg gcgacataat
     2041 ttcataagta tttttatttt tcagcatacc gtcttggtga atacctgaag agtgagcaaa
     2101 ggcattgcta ccgacaatcg ctttgttcgg ctgaatcggc atattgcaaa gctggctgac
     2161 catttggctg acacggtgaa tttcttgggt attaatgcgg gtatcaaccc ccataaattc
     2221 ttggcgggtt ttaattgcca ttaccacttc ttcaagagcg gtgttaccgg cacgttcgcc
     2281 aataccgttg atagtacact caatctgtct cgctccgttt tgcaccgctg tcaatgagtt
     2341 ggcggtagcc atacctaaat cgttatggca gtgaacagag acaatcgctt tatcgatatt
     2401 cggaacacgg ttcatcactt ggtggataat attgccgtat tcattcggta aacagaaacc
     2461 gactgtatcc ggaatattca ctgtggttgc accggcatta atggcagctt ctacaatgcg
     2521 gcagatgtta tcaattccag tacgcccagc atcttcacag gaaaactcaa catcatcagt
     2581 ataatttctg gcgcgtttta ccgctgcaac cgccatttcg actacatcat caaatgaacg
     2641 ttttaattta gcctcaacgt gtaatgccga gctggcaata aaggtatgga tacggaatgc
     2701 ttctgctact tttaaggctt cataagctgc atcaatgtct tttgcaaccg ctcgggaaag
     2761 cgccgccacc cggctgtttt tgatatgacg agcgatggtt tgtactgatt caaaatcgcc
     2821 cgctgaagaa actggaaaac cgacttccat cacatcaacc cctaaacgtt ctaaagcaag
     2881 ggcaatttgc aatttttctt ttacggttaa acttgctttt aatgattgct caccatcacg
     2941 taaagtcgta tcaaaaataa taatgttatt gttgctcata gaagcctctt tttaggtgtt
     3001 tttatttgat aaaaaaagcc cgcattttaa ggtgcgggca agaatgtgga tagtccattt
     3061 actttttcca atcaatcttt atcctcgcac aaaatacgat aacagagata atcggagtaa
     3121 ggtaaatttt tccatgttgt gttgtatgtt tatgtttgca ttgttattca gtagttgagg
     3181 tgtatattat ccttatccaa atcaatgtca atagcatttt tctcttcacg caaccgtt
//
LOCUS       DANLRK010000219         3171 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.220, whole genome shotgun sequence.
ACCESSION   DANLRK010000219 DANLRK010000000
VERSION     DANLRK010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3171
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.220"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(<1..129)
                     /gene="rpmG"
                     /locus_tag="PXA33_002413"
     CDS             complement(<1..129)
                     /gene="rpmG"
                     /locus_tag="PXA33_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005624479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDL5121705.1"
                     /translation="MAAKGAREKIRLVSTAETGHFYTTDKNKRNMPEKMEIKKFDPV"
     gene            complement(141..377)
                     /gene="rpmB"
                     /locus_tag="PXA33_002414"
     CDS             complement(141..377)
                     /gene="rpmB"
                     /locus_tag="PXA33_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004997628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HDL5121706.1"
                     /translation="MSRVCQVTGKRPAVGNNRSHAMNATKRRFLPNLHTHRFWVESEN
                     RFVTLRLTAKGMRIIDKKGIDAVLAEIRARGEKI"
     gene            complement(609..1346)
                     /gene="radC"
                     /locus_tag="PXA33_002415"
     CDS             complement(609..1346)
                     /gene="radC"
                     /locus_tag="PXA33_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HDL5121707.1"
                     /translation="MEEKMNDFFDEDSQEPTMMPREKLLTQGAASLTDSELLAIFLRT
                     GTKAVPVLALAESVLMAFGSLRQLLNANIDDFCKIYGLGKTKYIQLQASKEMTKRYLA
                     QQMEFSEMIQAPYMAIMYFQTELEEEEREVFMVLFLDNQNRLIYKEKMFFGTINQTAV
                     HPREIIKRALKYNAAAIIVAHNHPSGSCLPSESDRSLTKKIEMACELVDIRFVDHIIV
                     GKGDYFSFEEEKLEMKEHSFLQISDRK"
     gene            1496..2698
                     /gene="coaBC"
                     /locus_tag="PXA33_002416"
     CDS             1496..2698
                     /gene="coaBC"
                     /locus_tag="PXA33_002416"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HDL5121708.1"
                     /translation="MLQNKKILVGISGGIAAYKTIEFIRRLKKANAEVRVVLTPAAEA
                     FVTPLTLQAISGNAVSTSLLDPQAELAMGHIELAKWADLIVIAPASADFIARLRMGMG
                     NDLLSTLCLATASPILLAPAMNQQMYKQIAVQENLSVLVNRGVQLIGPNSGFQACGDV
                     GAGRMSEPEEIYQAVLAHFSNNQDLEGLNVTITAGPTREAIDPVRYISNHSSGKMGYA
                     IANAFAERGAKVTLISGPVNLPTPKNVERINVESAVEMEQQAVKFAQKSAIFIGCAAV
                     ADYRMAAVSDQKIKKTDANDELTLKLVKNPDIIANVANLAENRPFVVGFAAETQNVAE
                     YAKSKLERKNLDMICANDVSGGQVFGQDQNALHLFWKNGEKTLPLADKSKLAESLVQE
                     IVKHYREK"
     gene            2698..3015
                     /locus_tag="PXA33_002417"
     CDS             2698..3015
                     /locus_tag="PXA33_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121709.1"
                     /translation="MKLLVSHFHQGEGKFPTFSAGTAVRLGSECVNYPNWFACEIDGW
                     KTFVPSHFISEDKLICDYNPTELVAAQGERVEFLELHYQWAIVKRENEIGWLPCEILR
                     VIG"
     gene            3012..>3171
                     /locus_tag="PXA33_002418"
     CDS             3012..>3171
                     /locus_tag="PXA33_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121710.1"
                     /translation="MKIMQLTSNQYINDIKHILLSARQKAYQAVNSAMVEAYWKIGER
                     IVQEEKNMV"
BASE COUNT          935 a          664 c          669 g          903 t
ORIGIN      
        1 aactggatca aattttttga tttccatttt ttcaggcata ttacgtttgt ttttatctgt
       61 tgtgtagaag tgaccagttt ctgctgtaga aactaaacgg attttctcac gagcgccttt
      121 agctgccatt ttttagctcc ttagattttt tcgccacgag cacggatttc agctaatact
      181 gcatcaatgc cttttttatc aataatacgc atacctttcg cagttaaacg taaggttacg
      241 aaacggtttt cgctctcaac ccagaaacgg tgagtgtgaa ggtttggtaa gaaacgacgt
      301 ttagtcgcat tcattgcgtg tgagcggttg ttaccaactg ctggacgctt gccggttact
      361 tggcaaactc ttgacatttg aatttctcca ataattttaa gcttaaaaat ggttcgggcg
      421 atctcacaaa aggcgttgct caaccaaccc ctccgatttt gattgcctcg tcaggcatta
      481 acccgaccac agtctatgta ataaagactg ccgattatac cgatttcatt tgttatattc
      541 aagtagaatt tatatgcgta tcttttactg tttcaggaga aatgttaaag gttacataaa
      601 gcaagcggtc attttctatc agaaatttgc aaaaatgaat gctccttcat ctctaatttc
      661 tcttcctcga acgaaaaata gtcccctttg ccgacaataa tatgatccac aaagcgaata
      721 tcaaccaatt cgcacgccat ttcgatcttt ttagttaaac ttctgtccga ctcactcggc
      781 agacaagaac ctgaggggtg attatgagcc acaataatcg ccgcggcatt atattttaac
      841 gctcttttaa taatttctcg ggggtgaaca gcagtttgat taatggtgcc gaagaacatt
      901 ttttctttat aaatcaatcg gttttggttg tccaaaaaca gcaccataaa aacttcccgt
      961 tcttcttcct ccaactcggt ttggaaatac ataatcgcca tataaggggc ttgaatcatc
     1021 tcgctaaatt ccatttgttg agccagatag cgtttggtca tctctttaga ggcttgtaac
     1081 tgaatgtatt tggttttgcc taaaccgtaa attttgcaaa aatcatcaat atttgcattc
     1141 agtaattggc gtaacgagcc aaatgccatc aacacactct ctgccaatgc cagcacaggc
     1201 acagctttcg taccagtccg taaaaatatc gctaataatt cggaatcggt taatgaggct
     1261 gccccctgtg ttaataattt ctcacgcggc atcattgttg gctcttgaga atcttcatca
     1321 aaaaaatcgt tcattttttc ctccatcaat ttagttaagc ataaaagagc caacctttgc
     1381 agacaaaggg atttattcaa tttttcgagc aagatcgcaa aaatagtgaa gttgtgagca
     1441 actccttatt ccgctaaaat atgcaaaata aatccacgct ttaaggatag taaagatgtt
     1501 acaaaacaaa aaaattttag tcggcatttc cggtgggatt gcagcctaca aaaccattga
     1561 atttatccgc cgcttaaaaa aggcaaatgc ggaagtgcgt gtagtgctaa ccccagcagc
     1621 agaagccttt gtaacgcctt tgaccttaca agcgatttcc ggcaatgcgg tttccacttc
     1681 actactcgac ccacaagccg aactggcgat ggggcatatt gagctggcaa aatgggcgga
     1741 tttaatcgtt atcgccccgg caagtgccga ttttattgct cgcctgcgta tgggaatggg
     1801 caacgattta ctttccactc tctgccttgc gactgcaagc ccgattttgc ttgcaccggc
     1861 aatgaatcaa caaatgtaca aacagattgc ggtgcaagaa aatctgtctg ttttagtcaa
     1921 tcgtggcgtt cagctcatcg gcccgaatag tggcttccaa gcctgtggtg atgtgggggc
     1981 tggcagaatg tctgagcctg aagaaattta ccaagcggtg ttggctcatt tctccaacaa
     2041 tcaagattta gagggcttaa atgtaaccat taccgccgga ccgactcgtg aggcgattga
     2101 ccctgtgcgt tatatcagca accacagctc cggcaaaatg ggctacgcga ttgctaatgc
     2161 ctttgccgaa cgtggagcaa aggttacgtt gattagcggt ccggtgaatt taccaacgcc
     2221 gaaaaatgtg gaacgaatta atgtggaatc tgcggttgag atggagcaac aagcggtcaa
     2281 atttgcccaa aaatctgcga tttttattgg ctgtgcagcg gttgccgatt accgaatggc
     2341 ggcagtctct gaccaaaaaa ttaagaaaac cgatgccaat gatgaactca cgctaaaatt
     2401 ggtaaaaaac cccgatatta tcgctaacgt cgccaatctt gccgaaaacc gcccttttgt
     2461 ggttggtttt gccgcagaaa cgcaaaatgt ggcggagtac gctaaatcga aactcgaacg
     2521 caaaaatctg gatatgattt gtgccaatga tgtctcaggc ggacaagtgt tcgggcagga
     2581 tcaaaatgcg ttgcatttgt tctggaaaaa tggtgaaaaa acgcttcctc tggcggataa
     2641 aagcaaattg gcagaaagtt tggtacagga aattgtaaaa cattatcgag aaaaataatg
     2701 aaattactgg ttagccattt tcatcaaggc gaagggaaat ttcctacatt ctcagctggt
     2761 actgccgttc gattagggag tgaatgtgta aactacccaa actggtttgc ctgtgaaatt
     2821 gacggttgga aaacctttgt gccaagccat tttattagcg aagataaatt gatatgcgat
     2881 tataacccaa cggaacttgt tgcagcacaa ggcgagagag tagaattcct tgaactacat
     2941 taccaatggg caattgtcaa aagagaaaac gaaattggct ggctaccttg tgaaatctta
     3001 agggtaatag gatgaaaatt atgcaactta cgtcaaatca gtatatcaac gacattaaac
     3061 atattctttt atctgctcgc caaaaggcgt atcaagccgt gaattcggca atggtcgaag
     3121 cctattggaa aatcggtgaa cgaattgtgc aagaagagaa aaatatggtc a
//
LOCUS       DANLRK010000220         3147 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.221, whole genome shotgun sequence.
ACCESSION   DANLRK010000220 DANLRK010000000
VERSION     DANLRK010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3147
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.221"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            6..842
                     /locus_tag="PXA33_002419"
     CDS             6..842
                     /locus_tag="PXA33_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012621753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDL5121711.1"
                     /translation="MLFACINTLIPKLTSISPIDSNVIALVQYLAAFIFLVPSMNNIG
                     FFSSLKTEHFGQHCFRIFLSAIGIQCWTMALANQLPIWQGIALLMTSPLFVTIGSGLF
                     LKEKVDKKRWIATAMGFVGAMIILEPWSDHFEMIALLPIAAAFFWAGYSLMVKKLSRY
                     DSPTTMVAYLFILITPFNLLIALTNLSPSGFSLPSWSDFGFLILLGFFTALAQLAVAK
                     AYSVADASYIQPFDFIKLPLNVLAGWLVFNWVPPGKLWLGAAIIIAATIYITHAETKQ
                     SN"
     gene            complement(921..1412)
                     /gene="ilvN"
                     /locus_tag="PXA33_002420"
     CDS             complement(921..1412)
                     /gene="ilvN"
                     /locus_tag="PXA33_002420"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005647229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HDL5121712.1"
                     /translation="MRRTLAVLLENESGALSRVVALFSQRGFNIESLTVAPTDDPSLS
                     RMTIVAQGEEQVLEQIEKQLHKLIDVFKVSNLSAYEHVEREVLLVKVRATGSSRDELK
                     RMVDIYRGQIVDLTPKLYTIQLSGSTEKLNAFIEAVKEETTIVEIVRSGVISLSRGDK
                     NAL"
     gene            complement(1405..3126)
                     /locus_tag="PXA33_002421"
     CDS             complement(1405..3126)
                     /locus_tag="PXA33_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HDL5121713.1"
                     /translation="MKKLSGAEMIVQSLKDEGVEYVFGYPGGSVLDIYDAIHTLGLNH
                     VLVRHEQGAVHMADGYARSTGKVGCVLVTSGPGATNTITGILTAYTDSVPLVILSGQV
                     PSSLIGTDAFQESDMIGISRPVVKHSFLVKNTEEIPEIIKKAFYIASTGRPGPVVIDI
                     PKDVVNPLNKYPYEYPQEVSMRSYNPTVQGHKGQIKKALKALLVAKRPVLYVGGGVIS
                     AEGSPEITEFAQKLNLPVTCSLMGLGAYPASDKQYLGMLGMHGTFEANNAMHESDLIL
                     GIGVRFDDRTTNNLAKYCPNAKVIHVDIDPASISKTVQAYIPIVGSAKDVMEEFLSLL
                     GEENLAKNQVDLTAWWQQIDAWKARNCLAFEENSDVIKPQQVIRMIHKLTNGEAYVAS
                     DVGQHQMFAALHYGFDKPRRWINSGGAGTMGFGLPAAMGVKFAHPDATVVCVTGDGSI
                     QMNIQELSTAKQYDTPIVIVSLNNRFLGMVKQWQDIIYSGRHSQVYMNSLPNFAKLAE
                     AYGHVGIQIDHPSELEAKLTEAFSIKDKLVFVDVLVDETEHVYPMQVRGGAMNEMMLS
                     KTERTDA"
BASE COUNT          869 a          689 c          591 g          998 t
ORIGIN      
        1 ccggtgtgtt atttgcctgt atcaatacct taattcccaa attaacgtcc atttcaccga
       61 ttgactcgaa cgtgattgca ttggtgcaat atttagctgc ctttattttc ctcgttccca
      121 gcatgaataa tatcggtttc ttctcttcat taaaaaccga gcattttggg caacactgtt
      181 tccgtatttt tctatctgcg attggtattc aatgttggac aatggcactc gctaaccaat
      241 tacctatttg gcagggaatt gcgttattga tgacttctcc attgtttgtg accatcggct
      301 ccggtctgtt tttgaaagag aaagtggata aaaagcgttg gattgcaacc gcaatggggt
      361 ttgttggggc gatgattatt ctagagcctt ggagcgatca ttttgaaatg attgcgttac
      421 tgccgattgc cgcggctttt ttctgggcag gttattcgct aatggtgaaa aaactctctc
      481 gttacgatag cccaactacg atggtcgctt acctgtttat tctaattacg ccttttaatt
      541 tgttgattgc actcacgaac cttagcccta gtgggtttag tctgccttct tggagcgatt
      601 tcggcttttt aatcttactt ggtttcttta cggcgttagc gcaactggcg gtagccaaag
      661 cctacagtgt agcagatgct tcctacattc agccgtttga ttttattaaa ttacctttga
      721 acgtattagc cggttggctt gtatttaact gggtgccacc gggtaaatta tggctaggag
      781 ctgcgattat tattgctgcg accatttata ttactcacgc tgaaactaaa cagagtaatt
      841 agcaacaaat tcacgctaca taaaaatccc tttatctcaa agataaaggg atttgcaaat
      901 tttaagctaa aattgaccgc ttacagagcg tttttatcgc cacgagatag actaatcacg
      961 ccagagcgaa caatctctac aatcgttgtt tcttctttaa ctgcctcaat aaatgcattg
     1021 agcttctcgg tagaaccgga taattgaatg gtgtaaagtt tcggtgttaa atctacgatt
     1081 tgcccacggt aaatatctac catacgtttc agttcatcac gagatgaacc tgtagctcgc
     1141 acttttacca ataacacttc acgctcaacg tgttcataag cacttaagtt actcacttta
     1201 aacacatcaa tgagtttgtg tagttgcttt tcgatttgtt ctaacacctg ttcttcgcct
     1261 tgcgcgacaa ttgtcatacg ggaaaggctt ggatcatcag taggggcaac ggtcaagctt
     1321 tcaatgttaa aaccacgctg cgagaaaagg gcaactacac gagataatgc acctgattca
     1381 ttttctaata atacggctaa tgttctacgc atctgttctc tccgttttac ttaacatcat
     1441 ttcattcatt gctcctccac gcacttgcat tgggtaaaca tgttccgttt catcgaccaa
     1501 tacatcaaca aatacaagct tatctttaat tgagaatgcc tcagttaatt ttgcctccaa
     1561 ttcagaaggg tggtcgattt gaatcccaac gtggccataa gcctctgcta atttagcaaa
     1621 gttaggtaga gaattcatat acacctgtga atggcggcca gagtaaataa tatcttgcca
     1681 ctgtttcacc atacctaaga aacggttatt taagctgaca atcacaatcg gtgtgtcgta
     1741 ttgctttgcg gtggaaagtt cctggatatt catttggata ctaccatcac cggttacaca
     1801 aacaacagtg gcatcagggt gggcaaattt tacccccatc gcagcaggta agccaaagcc
     1861 cattgtgcct gcaccgcctg aattaatcca acgacgtggt ttatcgaacc cataatgcaa
     1921 tgcagcaaac atttgatgtt gtccaacatc tgaggcaaca taagcctccc cattggtcaa
     1981 cttgtggatc attctaatca cttgttgtgg cttaatcaca tcactgtttt cttcaaaggc
     2041 taagcaatta cgagctttcc aagcatcaat ttgttgccac caagcggtta aatctacctg
     2101 attttttgca agattttcct cacctaataa agataaaaat tcttccatta catccttagc
     2161 actgcctaca attggaatat aagcttgtac tgttttggaa attgaggctg gatcaatatc
     2221 cacgtggatc acttttgcat tcgggcaata ttttgctaag ttatttgttg tacgatcatc
     2281 aaaacgtacc ccaataccta aaatcaaatc actttcgtgc attgcattat ttgcctcaaa
     2341 agtgccgtgc atgcctaaca tacctaaata ttgcttatca gaggcaggat aagcacctaa
     2401 tcccattaat gaacaggtta caggtagatt tagcttttgt gcaaactctg taatttctgg
     2461 cgaaccctct gcactaatta caccaccgcc tacataaagc actggacgtt ttgctactaa
     2521 tagtgctttt aatgcttttt taatttgccc tttatgccct tgaactgttg ggttataaga
     2581 acgcattgat acttcttgcg gatattcata gggatattta tttaatggat tgactacatc
     2641 tttaggaata tcaataacta ccgggcctgg tcgaccggtt gatgcaatat aaaaggcttt
     2701 tttgataatt tcaggaattt cttctgtatt ttttactaag aaactgtgtt ttacaaccgg
     2761 acgagaaatg ccgatcatat cactttcttg gaatgcatct gtcccaatta aacttgatgg
     2821 aacttggccc gacagtatta ctaacggaac agagtcggta taagcagtta aaataccagt
     2881 aattgtattc gttgctcccg gacctgaagt cactagcaca cagccgactt tacctgttga
     2941 acgggcatag ccatcagcca tatgtacagc accttgctcg tgtcgaacta aaacgtgatt
     3001 caatccaagt gtatgaattg catcataaat atcgagtaca gagccacccg gatagccgaa
     3061 aacgtattct acgccttcat cctttaagga ctgtacaatc atttctgcac cagaaagttt
     3121 tttcattgaa tcctccaaca aaatagc
//
LOCUS       DANLRK010000221         3130 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.222, whole genome shotgun sequence.
ACCESSION   DANLRK010000221 DANLRK010000000
VERSION     DANLRK010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3130
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.222"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            110..2149
                     /gene="malQ"
                     /locus_tag="PXA33_002422"
     CDS             110..2149
                     /gene="malQ"
                     /locus_tag="PXA33_002422"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HDL5121714.1"
                     /translation="MSNKQNTAHYFYSPNIKEYASPSAFEKIERLLNTDNDLQKNNQI
                     LPLVKVITQPNAEQQAVEISLNFTNVAKWQIVLENGEVRNGEIKAEQSVLTLPADLPL
                     GYHQLSISTENDEESCSLIVTPPKAFQPKPLEERKKLWGAFLQLYTLRSDNNWGIGDF
                     GDLKQFLANISDYGADFVGLNPIHALFPADPDAASPYSPSFRLWQNIIYIDVSAVEAF
                     QQSEQAQQWFHTKETQQQLAELRAKDFVDYSQVAQLKLKALQFAFEHFSQSSQQQSQT
                     AFDAFIEQAGEPLKIQATFDALHHWISSEFGEKGGWTNWAEEYQSYYSPKVAEFQISQ
                     ATQIRFYMWLQFVANQQLAECNRFAQSLSMPIGFYRDLAVGVTQSGAETWADKALFVQ
                     EASIGAPPDMLAPNGQSWGLSPMHPHILQSRGYQPFIDLVRANMKHCGALRIDHILGF
                     ARMWWVANGESAKEGIYVRYPLEDLLSILALESQRHQCLVIAEALGIVPEGILDSLEQ
                     KGIFAYNVFYFEKAENAFKPLENYPYQAMTTLSTHDLPTIQGYWKGYDFELGEKFDTY
                     PSKAVLRKLKEERSYDRLNIRNAVEKVIALEPNEVGVTIKFTHQLQRYVAHTHSALFG
                     TQPEDWLNMLEPVNIPGTSTEYPNWRRKLSATTEQIFANKDIQQLLKDINEIRKG"
     gene            complement(2217..2948)
                     /locus_tag="PXA33_002423"
     CDS             complement(2217..2948)
                     /locus_tag="PXA33_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HDL5121715.1"
                     /translation="MGCSSFSTKLKQEFFSGWTTFEASWLIVFLAIQIGIFIYQPDTW
                     IATIAAITGILCVVFVGKGKISNYLFGLISVSLYAYISYTFQLYGEMMLNLLVYVPVQ
                     FIGFYFWRKNMTSENTVNNAGVEEVIAKALTAKQWVIVAITTIIGTFLYIELLKYLGS
                     ALAILDGATVVISIVAQILMVLRYREQWALWIIVNIMTISLWTAMYFQNGETSLPLLV
                     MYVMYLCNSIYGYYNWIKLHRKHQQ"
BASE COUNT         1024 a          650 c          587 g          869 t
ORIGIN      
        1 tttttttaac atcatatcac aaagacaaac ctgttgccta ctcttcttac tcatattaat
       61 cttcacaaga tcgttatttt aacaaataca gtaaaggaaa aaagtcataa tgtcaaacaa
      121 acaaaataca gctcactatt tttactcccc aaatattaaa gagtatgcca gcccctctgc
      181 ttttgagaaa atagagcggc ttttaaatac cgataacgat ttgcaaaaaa ataatcaaat
      241 tctaccgctt gttaaagtga ttactcagcc aaatgccgag caacaagcgg tcgaaatttc
      301 gctgaatttt acaaatgtag caaagtggca aattgtgctt gaaaatggtg aggttcggaa
      361 cggtgaaatt aaggcagagc aatcagttct aaccctgcct gccgatttgc ctttaggcta
      421 tcatcagctt tctatttcaa ctgaaaatga tgaagaatct tgctcgctga ttgtaacacc
      481 gccgaaagct ttccagccta agccattgga agaaaggaaa aagctctggg gtgcattttt
      541 acagctttat actttgcgtt ccgataacaa ctggggaatt ggcgattttg gcgatctcaa
      601 acaattcctt gccaacattt ctgattatgg tgcagatttt gtgggcttaa accctattca
      661 cgccctcttt ccggcagatc ctgatgcggc aagcccttac agcccgtcct ttcgactttg
      721 gcaaaatatt atttatattg atgtttcagc agtagaagcc tttcaacaat ctgaacaggc
      781 tcaacaatgg tttcacacca aagaaactca acagcaactt gccgaattgc gagccaaaga
      841 ttttgtcgat tacagccaag tagcgcaact gaaattaaag gctctgcagt ttgcttttga
      901 gcatttttca cagtcatctc aacaacaatc gcaaacagcg ttcgatgcct ttattgagca
      961 agcaggcgag ccattaaaaa tccaagcaac gttcgatgct ttgcaccact ggatttcatc
     1021 tgaatttggc gaaaaaggcg gctggacaaa ctgggcggag gaatatcaaa gttactattc
     1081 gcctaaagta gctgaatttc aaatatcgca ggcaacgcag atacgttttt atatgtggtt
     1141 gcaatttgtg gcaaaccagc aattagcaga atgcaaccgg tttgctcaat cactttcaat
     1201 gccgattggt ttttaccgcg atttagccgt tggtgttact caaagcggag cggaaacttg
     1261 ggcggataaa gccctatttg tgcaagaggc gtcaatcggt gcaccacctg atatgcttgc
     1321 cccgaacgga cagagctggg gactttcgcc aatgcacccg catattctac aaagccgagg
     1381 ttatcagccg tttattgatt tagtgcgtgc taatatgaaa cattgtgggg cattgcgaat
     1441 cgaccatatt ctcggcttcg cccgtatgtg gtgggtggcg aacggtgaat cggcaaaaga
     1501 agggatttat gtccgttatc cgctcgaaga tttgctctct attttggcat tagaaagcca
     1561 acgtcaccaa tgtttagtga ttgcagaggc acttggcatt gtgcctgaag gcattttgga
     1621 ctctctcgag caaaagggca tttttgccta caatgttttt tattttgaaa aggcagaaaa
     1681 cgcgtttaaa ccgctggaaa attatcctta ccaagcgatg acaactctaa gcacccacga
     1741 tttaccgact attcaaggct attggaaagg ctatgatttt gagctaggcg aaaaatttga
     1801 cacttaccca agcaaagccg tattgagaaa gctgaaagaa gagcgttcgt acgaccgctt
     1861 gaatatccgt aatgcggtgg aaaaagtgat agcactcgag ccaaatgaag tgggagtaac
     1921 aataaaattt acccaccaat tacaacgcta tgtggctcac actcacagtg cattattcgg
     1981 tacacagccc gaagattggc tcaatatgtt agagccggtg aacattccgg gaaccagcac
     2041 cgaataccct aactggagaa gaaaattatc cgcaaccacc gagcagattt ttgcaaataa
     2101 agatattcaa caactgctaa aagatataaa cgaaattcgt aaaggataat aacaagcggt
     2161 catttttagg taaattttta caaaaaatga ccgcttgtaa tttgtattta ttcgctctat
     2221 tgctgatgtt ttctgtgaag tttaatccag ttgtaataac cgtaaataga gttacataaa
     2281 tacatcacat acatcaccaa cagcggcaag ctggtttcgc cgttttggaa gtacattgcc
     2341 gtccataaag aaatggtcat aatattcacg ataatccaca atgcccattg ctcacggtag
     2401 cgaagtacca tcaaaatttg tgccacaatc gaaataacca ctgttgcacc atctaatata
     2461 gctaacgcac tgcctaaata tttaagcaat tcaatataaa gaaatgtgcc gataatggtg
     2521 gtaatggcga caatcaccca ctgttttgcg gttaaagctt tagcaattac ctcttccaca
     2581 ccagcattat ttaccgtgtt ttcgctggtc atatttttac gccagaaata gaaaccgata
     2641 aactgcaccg gcacatatac aagtaaattc agcatcattt caccatataa ctgaaaggta
     2701 taagagatat aagcataaag cgaaacggaa attaagccga atagatagtt actgattttt
     2761 cctttaccga caaataccac gcataaaata cctgttattg ccgcaatagt tgcaatccaa
     2821 gtatcgggtt gataaataaa aataccgatt tgaatcgcta aaaaaacaat caaccaactt
     2881 gcctcaaaag tagtccaacc actaaaaaac tcttgtttta acttagttga aaaagatgaa
     2941 cagcccatat ttcctccata taaagtgaag tattcttttc aaattctaag ttatttaaat
     3001 taagaaatca aaatacagag taccttttga taaatatacg aaacatttat tatattaata
     3061 aacctatatt taattggcat ttaactaaat cagtgttttt taaaactaaa aaacatacca
     3121 aaaaccaaat
//
LOCUS       DANLRK010000222         3114 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.223, whole genome shotgun sequence.
ACCESSION   DANLRK010000222 DANLRK010000000
VERSION     DANLRK010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3114
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.223"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(8..295)
                     /gene="ybgE"
                     /locus_tag="PXA33_002424"
     CDS             complement(8..295)
                     /gene="ybgE"
                     /locus_tag="PXA33_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HDL5121716.1"
                     /translation="MIHKLYNLTAKGLLKALSFILAAVLFATIWVNSTAFALSFGGKT
                     PYLAMLVFYGMAILWVHGIGFEIRAAIWKVIFLPLLGYLIVIPALWILLVK"
     gene            complement(295..393)
                     /locus_tag="PXA33_002425"
     CDS             complement(295..393)
                     /locus_tag="PXA33_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CydX/CbdX family cytochrome bd oxidase small
                     subunit"
                     /protein_id="HDL5121717.1"
                     /translation="MFYVTWVLGVLLAIFFATVITISIEKTGKFDE"
     gene            complement(408..1541)
                     /gene="cydB"
                     /locus_tag="PXA33_002426"
     CDS             complement(408..1541)
                     /gene="cydB"
                     /locus_tag="PXA33_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HDL5121718.1"
                     /translation="MIDYEILRFIWWILIAVLLIGFAITDGFDMGVLNLLPIIGKSNV
                     ERRIMINTIAPHWDGNQVWLLTAGGAIFAAWPTVYATSFSGFYIAMVLVLAALFFRPV
                     GFEYRAKIDNPKWRSAWDWGLFIGGFVPSLVFGVAFGNLLQGVPFEFNNINQSQYTGS
                     FFGLLNPFALVCGIISLMMLTTQGATWLQMKTTDELHNNSRKVAQVTASIVLVAFILA
                     GVWLYFKDGFVITSELDHNAQSLLTNKTVAVQAGAWFRNYAEMPVLWVVPALVVVGAL
                     LTIIASKANRSGLAFFSSSLMIVGVILTAAVSMFPFIMPSITHPEMSLTVWDATASHT
                     TLTVMLFVAGIFVPLVLAYTIWSYIKMYGRLDASYLEENKAAY"
     gene            complement(1554..3104)
                     /locus_tag="PXA33_002427"
     CDS             complement(1554..3104)
                     /locus_tag="PXA33_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HDL5121719.1"
                     /translation="MLDVVELSRLQFALTALYHFLFVPLTLGLSFVLVVMETLYVTTG
                     KEVYKDMTKFWGKLFGINFALGVTTGITMEFQFGMNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWERLSKAKHLLATYCVAFGSNFSALWILVANGWMQNPIASEFN
                     FETMRMELSSFSELVLNPVTQSKFLHTVTAGYVCGAIFVLGISSYYILKGRDLGFAKR
                     SFSVGASFGLIAIISVLIMGDESGYEIGKAQPTKLAAMEGEFETQPAPAAWNAFVIPN
                     TAEMKNEFSIHIPYAAGIIATRSLDKEIKGLKDIRVINEQRVRTGMVAYGLLEKLRSG
                     NYTEADKEAFKAVQKDLGFGLLLKQYTPNVVDATEEQIQKAADNTIPNVGPTFWAFRV
                     MMLSGFLMLILITTAFIQNMRGKVESSRFLLRGLLFGLPLPWIAIESGWFLAEYGRQP
                     WAIYEMLPVGVSNSALTTGDLWFTIGLLCGLYTLFLVVEMYLMFKYGRLGPSALKTGR
                     YYFEQSTK"
BASE COUNT         1108 a          648 c          505 g          853 t
ORIGIN      
        1 aatgcctcta tttaacaagt aaaatccata atgccggaat aacaataagg taacctagaa
       61 gtggtaaaaa aatcactttc caaatagcag ctcggatttc aaatccaatt ccatgtaccc
      121 ataaaatagc cataccataa aataccaaca ttgctaaata cggcgttttt ccaccaaaag
      181 aaagagcgaa agcggtcgaa tttacccaaa tagttgcaaa taatacagcc gctaaaataa
      241 aagaaagggc ttttaacaag ccctttgctg ttaagttata gagtttatga atcattactc
      301 gtcaaactta cctgtttttt caatgctgat cgtgatgact gttgcaaaga agattgcaag
      361 caatacacct aagacccaag ttacataaaa catattgtgt ctccttatta gtaagctgct
      421 ttattttctt ctaaataact agcatctaaa cgaccataca ttttgatata tgaccaaata
      481 gtatatgcta atactaacgg tacaaagata ccagcaacaa ataacattac agttaatgtt
      541 gtgtggctag cagtagcgtc ccaaaccgtt aagctcattt ctggatgagt aattgacggc
      601 ataatgaatg ggaacattga tactgcagca gttaaaatca ccccaacaat catcaatgat
      661 gatgagaaga atgctaaacc tgaacgattt gcttttgagg caataatagt cagtaatgca
      721 cctactacaa ctaatgcagg aacaacccat aatacaggca tttcagcata atttctaaac
      781 catgcaccag cttgaaccgc tacagtttta ttcgtcaata gagattgtgc attatgatct
      841 aactcgcttg tgatcacaaa accatcttta aaatataacc atacacctgc aagaataaat
      901 gccactaata caatagaagc tgtcacttgt gccacttttc ttgagttgtt atgtagctca
      961 tcagtagttt tcatttgtaa ccaagttgca ccctgtgtgg ttaacatcat taagctgata
     1021 ataccgcaca ctaatgcaaa tggatttaat aaaccaaaga atgaacccgt atattgtgat
     1081 tggttaatat tattaaattc aaatggtaca ccttgtaata agttaccgaa tgcaacaccg
     1141 aatactaatg atggaacgaa accaccaata aataatcccc aatcccaagc actacgccat
     1201 ttcggattat caatttttgc acgatactca aaacctacag ggcggaaaaa taatgctgca
     1261 agcacaagca ccattgcaat atagaaaccc gagaacgaag ttgcatagac tgttggccat
     1321 gcagcgaaga tcgcaccacc agcggtcaat aaccatactt ggttaccatc ccagtgaggg
     1381 gcaattgtgt tgatcataat acgacgttca acattacttt taccaatgat aggtaaaagg
     1441 tttaatacgc ccatatcaaa accatcagta attgcaaagc caattaacaa taccgcaatt
     1501 aagatccacc aaataaagcg gagaatttca taatcgatca tttctaactc ctattattta
     1561 gttgattgtt caaaataata acggcctgtt tttaatgcac tagggccaag acgaccatat
     1621 ttgaacatta aatacatttc tacaactaag aatagtgtat ataaaccaca caataagccg
     1681 attgtaaacc ataaatcacc tgttgttaat gcagagtttg acacaccgac aggtaacatt
     1741 tcatagatag cccaaggctg acggccatat tcggcaagga accaaccaga ttcaatcgca
     1801 atccaaggta acggaagacc gaataacaag cctcttaata agaaacggct actttctact
     1861 ttaccacgca tattttgaat aaatgctgtc gtaatcaaga taagcatcaa gaagccagat
     1921 aacatcatta cacggaatgc ccagaatgta gggcctacat ttggaattgt attatctgct
     1981 gctttttgaa tttgttcttc agtcgcatca accacattag gtgtatattg ttttaacaat
     2041 aacccaaagc ctaaatcttt ttgtacagct ttaaatgctt ctttatccgc ctctgtataa
     2101 ttgccagaac gtaatttttc taatagtccg taggctacca taccggtacg aacacgttgt
     2161 tcattaatta cacgaatatc ttttaggcct ttaatttctt tatctaaaga acgggttgca
     2221 ataataccag ctgcataagg aatatgaatt gagaattcat ttttcatttc cgcagtattt
     2281 ggaatcacaa aagcattcca agcagctgga gcaggctgag tttcgaattc accttccatt
     2341 gcagctaatt tagtcggctg agctttaccg atttcataac cagattcatc acccataatc
     2401 agtacagaga tgatcgcaat taaaccaaaa cttgcaccta ctgagaatga acgttttgca
     2461 aagcctaagt cacgaccttt taagatatag tatgaactaa tacctaatac aaaaatagca
     2521 ccacaaacat agcctgcggt tacagtatgt aagaatttag attgtgtaac tgggtttaat
     2581 acaagttcag agaaacttga aagctccatt cgcatggttt caaagttgaa ttcagaagca
     2641 atcgggtttt gcatccaacc attagcaact aaaatccata gtgcagagaa gttagaacca
     2701 aacgcaacac agtaagttgc taataaatgt ttcgctttcg ataaacgttc ccagccaaag
     2761 aagaacaagc ccacaaaggt agattctaag aagaacgcca ttaaaccttc aatcgctaat
     2821 ggagcgccaa aaatatcacc tacataatga gaataatatg accagttcat accaaactgg
     2881 aattccattg taataccagt tgttacccct agggcaaagt taataccaaa taatttaccc
     2941 cagaatttag tcatatcttt atatacttct ttacccgtag ttacatacag ggtttccatc
     3001 accacaagga cgaaagaaag acctaatgta agcggtacga ataagaagtg atagagtgca
     3061 gttagagcaa actgcaaacg tgagagttca actacatcta acatctcaaa cctc
//
LOCUS       DANLRK010000223         3095 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.224, whole genome shotgun sequence.
ACCESSION   DANLRK010000223 DANLRK010000000
VERSION     DANLRK010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3095
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.224"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            171..776
                     /locus_tag="PXA33_002428"
     CDS             171..776
                     /locus_tag="PXA33_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HDL5121720.1"
                     /translation="MSEPNHAEQIRRLDNIIRLGTIAEVDLTTATARVNSGGITTDFL
                     PWLTFRAGTSQTWSAPTVGEQCVVLSVSGEFTTGVILFGIYTQNAPSQSADEHLFRFS
                     DGAEISYNTASGQLTVKNCKVVIVQATESITLDTPTVKATKNVVIGGNLSVKGTTSSQ
                     GAISTQGAVTAKGDVSGAGISLGSHHHIEQGDGRPTSGAKP"
     gene            776..1111
                     /locus_tag="PXA33_002429"
     CDS             776..1111
                     /locus_tag="PXA33_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HDL5121721.1"
                     /translation="MNRETGEQLEDEVEHIKQSIKDILLTAKGSRVMRRTYGSNLYKL
                     TDKPIAASLIMQLSAACVMALQQWEPRIAITRFKVEFAEDNYNKLICTLDFTLKHRKI
                     SIKGMRFTL"
     gene            1108..2022
                     /locus_tag="PXA33_002430"
     CDS             1108..2022
                     /locus_tag="PXA33_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate assembly protein"
                     /protein_id="HDL5121722.1"
                     /translation="MSEIVDLSKLPAPKVLEELDYETLLAERKAKFLSLYPESERAVM
                     AARLALESEPITKLLQENCYLQLLERQRINSAAQATMLAYATGTDLDVIAANFNVERL
                     VVQAEDLTASPPIVEELEPDDFRQRVQLKIESVSVAGPRKAYTFHALSANGKIADVSV
                     ISPQPAYVTVTLLSRDGNGAVASEIINAAKIALNDEEVRPIADRVTVQGASIVEYEIN
                     AVLHLYRGPEKEPILKAAKANLEAYINQTKRIGRDVTHSGIHAALHVAGVQNVEILQP
                     RTDLVLTPYQAGYCVNYTLNVEVSDEYS"
     gene            2009..2554
                     /locus_tag="PXA33_002431"
     CDS             2009..2554
                     /locus_tag="PXA33_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016533149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HDL5121723.1"
                     /translation="MNTVKALLPPGSSLLEKRAAECLQQAVQNPIKFANLINPHKCPV
                     KFLPYLAWALSVDYWEEHWSEELKRETIIRSFEEHRKKGTASAVRRIVESLGYAFEIR
                     EWFAEKRQRQPGTFRVFVELKDKGISEKIYQELERLIEDAKPVSRHITELAVSATSKG
                     KANIFSGAHSGEIITIYPNKR"
     gene            2557..>3095
                     /locus_tag="PXA33_002432"
     CDS             2557..>3095
                     /locus_tag="PXA33_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015981653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL5121724.1"
                     /translation="MEQKYKTILTHHGERVIVEALANKIPVPLKEMAIGDGNGSSITP
                     SASQTTLVREVYRAEITDLLEDPQNRHQMIAELLIPENVGGFIVREIGLFDEQGGLVA
                     VANCPENYKPVLEQGSGKVQYYRMILQVSSSDAVTLSINNNIVYATRTEFNEFVNNLS
                     SPDGYKHIGRCKSVAELRT"
BASE COUNT          952 a          670 c          715 g          758 t
ORIGIN      
        1 ttagctatca ttcacagtag ccgactttac ccacgcccat aatcagtaag cgacctaatc
       61 ggtcgctttt tctttgcctg ttaaaaccca tttcacacct cgcatttata cccaaagcct
      121 ttcgcctttt gcaaaatatc ctcatttttt aaccgcttgt aattgtaaaa atgagtgagc
      181 caaaccacgc cgaacaaatc cgccgtttag acaatattat ccgacttggc actattgccg
      241 aggtggattt aaccacagcg actgcacggg tcaattcggg cggtattacg accgatttct
      301 taccgtggct gacttttcgt gccggtacat cgcaaacgtg gtcagctcca acagttggcg
      361 agcaatgcgt ggtgttaagc gtgagtggtg aatttaccac aggcgtgatc ttatttggga
      421 tttacaccca aaacgccccg agccaatccg ccgatgagca tctattccgc ttttcggacg
      481 gtgcagaaat cagctacaac accgcaagcg gtcaattaac cgtaaaaaat tgcaaagtgg
      541 tgattgtgca ggcgacagaa tctatcacgc tcgacacgcc cacggttaag gccacaaaaa
      601 atgtcgtgat tgggggcaac ctatccgtga aaggcacaac ctcatcgcaa ggtgcaatca
      661 gcacacaagg ggcggtaaca gcaaaaggcg atgtatcagg ggcgggaatc agtttaggca
      721 gccaccatca cattgagcaa ggtgacggca gaccaactag cggagcaaaa ccataatgaa
      781 ccgagaaaca ggcgaacagc ttgaagacga agtagagcat atcaagcagt ctatcaaaga
      841 tattttgctg acggcaaaag gcagtcgggt aatgcggcga acctatggca gtaatctcta
      901 caagctaact gataaaccga ttgccgcaag tctgattatg caactctccg ctgcgtgcgt
      961 gatggcgttg cagcaatggg agccacgtat agcgatcacc cgatttaaag tggaatttgc
     1021 cgaagataac tacaataagc tgatttgcac cttagatttt acccttaagc accgaaaaat
     1081 cagcatcaaa ggaatgcgtt ttacactatg agcgaaattg tcgatttatc caaactcccc
     1141 gccccgaaag tactggaaga attggactat gaaaccttac tggcagagcg gaaagccaag
     1201 tttttatccc tctacccaga aagcgaacga gcggtaatgg ctgcacggtt agcgttggag
     1261 tctgagccta tcaccaagtt actacaagaa aattgctatt tgcagctact cgaacgccaa
     1321 cgcattaata gtgcggctca agccacaatg cttgcttatg ctaccggtac ggatttagat
     1381 gtcatcgcag ccaactttaa tgttgagcgg cttgtagtgc aagctgaaga cttaactgca
     1441 agcccgccta tcgtcgaaga gttagagcct gatgatttcc gtcagcgtgt gcagttaaaa
     1501 attgaaagtg tttcggttgc cggtccacgg aaggcttaca cctttcacgc cctgtctgct
     1561 aacggaaaaa ttgccgatgt gtccgtcata tcaccacagc cagcttatgt aacggtaacg
     1621 ctgctttctc gtgatggcaa cggagccgta gcctcagaaa tcatcaatgc ggccaaaatt
     1681 gctctcaatg atgaagaggt tagaccgatt gccgatagag taaccgtgca aggggcaagc
     1741 attgtggaat atgaaattaa tgcggtgttg catctctacc gtggacctga gaaagagcct
     1801 attttaaaag cggcaaaagc taacttagag gcttacatca accaaacaaa acgcattgga
     1861 cgagatgtga ctcactcagg tatccacgcc gccttgcacg ttgccggtgt gcaaaatgtg
     1921 gaaattttgc agccgagaac tgatttggtg ctgacacctt accaagccgg ttattgtgtc
     1981 aattacacgc taaatgttga ggtgagcgat gaatacagtt aaagcattat tgccacccgg
     2041 ttcatcactt ttggaaaaac gagctgccga atgtttgcaa caagcggtac aaaatccgat
     2101 taaatttgca aatttgatta atccacacaa atgccccgtg aaatttttgc cctatcttgc
     2161 gtgggcctta tcggtggatt attgggaaga gcattggagt gaagaattaa aaagagaaac
     2221 cattattcga tcttttgaag aacaccgaaa gaaaggcaca gcaagtgcag tgcgaagaat
     2281 tgtggaatct ctcggctatg cttttgaaat ccgtgagtgg tttgcggaaa aacggcaacg
     2341 gcagcccggg acgtttaggg tttttgttga gttaaaagat aaagggatta gcgaaaaaat
     2401 ctaccaagaa ttagaaaggt tgattgaaga tgcaaaaccg gtatcaaggc acatcacaga
     2461 attagcagta tcagccacct caaaaggcaa agcaaatatt tttagtggag cacacagtgg
     2521 cgaaattatt acgatttatc ctaataagag gtaaaaatgg aacaaaaata taaaacaata
     2581 ttaacccatc acggtgagcg agtgatcgtt gaggctcttg caaataagat accagtgcca
     2641 cttaaggaaa tggctatagg agatggtaac gggtcatcaa taactccaag tgcatctcaa
     2701 accacacttg taagagaagt ttatcgtgcg gaaattacag atttgctaga agacccgcaa
     2761 aatcgccatc agatgattgc tgaattacta atcccagaaa atgtaggtgg atttattgtc
     2821 cgagaaatag ggttatttga tgaacaaggt ggtttggttg ctgtagcaaa ttgccctgaa
     2881 aattataagc ctgtattaga acaaggcagt ggtaaagtcc aatattaccg aatgatttta
     2941 caagttagta gtagtgatgc ggtcacctta tcgattaaca ataatattgt ttacgccaca
     3001 agaactgagt ttaacgaatt tgtaaataac ttgagtagtc ctgatggcta taaacacatc
     3061 ggacgctgca aatctgtagc agaactccgc actat
//
LOCUS       DANLRK010000224         3014 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.225, whole genome shotgun sequence.
ACCESSION   DANLRK010000224 DANLRK010000000
VERSION     DANLRK010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3014
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.225"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            132..722
                     /locus_tag="PXA33_002433"
     CDS             132..722
                     /locus_tag="PXA33_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121725.1"
                     /translation="MTTQITTAQINDLLKSHDIQGYANEWQGRRIYINLSAKNNESRG
                     DRNYQLYIDLATGELVSKLGKGLTTREFDAAVKAFENAFNAKDTAENTSPAESKETEQ
                     ENKQETTKLVITKPTLKGSEKQIAWADEIINLFIAKIGDTSIPVTATQEQITQAQAMV
                     DNFFAMTNAGKWIEKFKGLNDSSEPKQFWLSVKYGW"
     gene            792..1310
                     /locus_tag="PXA33_002434"
     CDS             792..1310
                     /locus_tag="PXA33_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121726.1"
                     /translation="MTTQVTPQIMRIIGQIVAATYGDDVPTNVQTIILRYPIRGIGFI
                     SSRRELSINNGEIARLMDKIPGDLEDPKDGMPFDCQGAFWLGYYQYCKLSNDVKNYTS
                     KELSIIGESLYGTQWQSNLARDLRLSDARRVREWVAGERKIPFGVWADLTELVKAKKA
                     NLSSILKKLTID"
     gene            complement(1457..1696)
                     /locus_tag="PXA33_002435"
                     /pseudo
     CDS             complement(1457..1696)
                     /locus_tag="PXA33_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249173.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1857..2150)
                     /locus_tag="PXA33_002436"
     CDS             complement(1857..2150)
                     /locus_tag="PXA33_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121727.1"
                     /translation="MTTFNKILNPMYSTIASYSTQDDGSLNAKYVVGTGDDTDGEVTN
                     FVIITSEYKYIDAQSAKAITDAPLTKEDIGKTPTQIMLGRIYKHLKETGQIVV"
     gene            complement(2265..2864)
                     /locus_tag="PXA33_002437"
     CDS             complement(2265..2864)
                     /locus_tag="PXA33_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121728.1"
                     /translation="MSYGLSTFNINENFRSLSLQRKGRANFSNKIATIQAASDEIIVI
                     KYSDGQVCLLYRENGIATLATENATFAEYLIFSTNVPKSGGYGIEVYNSAGNVVFSSN
                     HKFLRPIEYVDLNPSKNVFRFTGQANRQYGIILSNFGFDFVINYDNGYSTSRSAKVSG
                     NFIEFGFCKYHYSGLYKTGMSYNSKAMYFNALVVDITGY"
BASE COUNT         1006 a          564 c          547 g          897 t
ORIGIN      
        1 cataaatttt ttaaaaaatt tgcaaaaaaa tttaaaaaaa ctcttgcaat aaaattatta
       61 tgcgcctata attaggctca taaagcaagg ctagggagcc agctaataac catcagccaa
      121 agggggctaa aatgacaact caaattacaa ccgcacaaat caacgactta ttaaaatctc
      181 acgatatcca aggctacgcc aacgaatggc aaggtcgccg catttacatt aacctttcag
      241 caaaaaataa cgaaagcaga ggtgatcgga attatcagtt gtacattgac ttagccacag
      301 gtgagttagt gagcaaactt ggcaaaggat taacaacaag agagttcgac gctgccgtaa
      361 aagcatttga gaacgctttt aatgctaaag acaccgctga aaatacatcg ccagcagaaa
      421 gcaaagaaac agagcaagag aacaaacaag aaacaactaa gcttgtaatc acaaaaccaa
      481 cattaaaagg ctctgaaaaa caaatcgctt gggcagacga aatcatcaat ctttttattg
      541 cgaagattgg tgatacctct atcccagtaa cagcaacgca agaacaaatt actcaagctc
      601 aagcaatggt agataatttt tttgcaatga caaacgctgg aaagtggatc gaaaaattta
      661 aaggtctaaa tgatagtagc gaaccaaagc aattttggtt atccgtaaaa tatggatggt
      721 agattgagat aacaaataag ccgtaagagc aaaggcttac ggctaaaaca aacagataac
      781 tggagattat tatgactaca caagtaacac cacaaattat gcgcattatc gggcaaattg
      841 tcgctgctac ttatggtgac gatgtgccaa caaacgtaca gacaataatc ttgagatacc
      901 ctataagagg gatagggttt atctctagtc gcagagagct atctattaac aatggagaga
      961 tagcaagatt aatggataaa ataccaggcg acctcgagga ccctaaagac gggatgcctt
     1021 ttgattgtca gggggcgttt tggcttggat actatcaata ctgcaaatta agtaacgatg
     1081 taaaaaatta cacatcaaaa gagctatcca tcattggcga gtcattgtat ggcactcaat
     1141 ggcagtctaa tctagcgaga gatttgagat tatctgatgc tcgccgtgtc cgcgaatggg
     1201 tggctggtga acgtaaaatc cctttcgggg tttgggctga tttgaccgaa ttggttaaag
     1261 ccaaaaaagc taacttatca tcaattctta aaaaattaac aatagattag cactgacttt
     1321 aaagcccctc tcggggctta attatagaaa ataaaatcac aatggctaat tttgccaaat
     1381 ttgcctgtta aaacgtgcga aaaattacaa aaaacggcta gagctatagc cgttttgagt
     1441 gcagatgtta acagagttaa gctctagcac gtcctgggcc ttgatactgc acaccatcag
     1501 ttaaatgatc ttgattttcg gttgctgcta cagcagattc tgatcttaca tttaaacttt
     1561 gtgactgttg taatgcatca ataagtttat ttaccactgt tccgagtgtt ttaatttgct
     1621 cggtttgctc attaatagtc tgctttaact cttgcagttt taatgattgc tcatccacaa
     1681 ccactaatcg acggttagct caatttgagg ctctgtgctg ccctctacta tggcgtgtaa
     1741 atccagccta ataccctcta aatttacagg ctcgccattt gtttgcctaa aaaaaaggcg
     1801 ataacttacg tcatcgcctc tgaaaaactt aaatttatgc ttttgcattt agacccctac
     1861 actacaatct gccccgtctc ttttaagtgc ttatagatac gacctaacat aatctgcgtt
     1921 ggcgttttgc caatatcctc ttttgttagc ggtgcatctg taatcgcttt ggctgattga
     1981 gcatcaatat atttgtactc acttgtgatg attacaaagt ttgttacctc gccatcggta
     2041 tcatcacctg tgccaacaac atacttagca ttgagtgagc cgtcatcttg agttgagtaa
     2101 cttgcgatag ttgagtacat tgggtttaag attttgttaa aagttgtcat aatttttcct
     2161 tttgtggatt gttgataaaa aaagaccgct tgtattgcta caagcggcga tgaatgtgta
     2221 atcagaattt tagttcgcct ctattaaatg aaggcgttta atgtttaata gcctgttata
     2281 tctacaacca gagcattaaa atacattgct tttgaattat aactcattcc cgtcttatac
     2341 aaaccagaat aatggtattt acagaaccca aattcaataa aattaccact tactttggca
     2401 gatctagatg ttgaatatcc gttatcgtag ttaatgacaa aatcaaagcc aaaattggat
     2461 aagataatgc catattgcct atttgcttgc cctgtaaatc taaaaacatt ttttgatgga
     2521 tttagatcga catattcaat aggtcttaaa aatttgtggt ttgatgaaaa tacaacattt
     2581 cctgctgagt tataaacctc tatgccgtaa cctccgcttt tcggtacatt tgtactaaaa
     2641 atgagatatt cagcaaatgt tgcgttttct gtagctaatg tggcaatacc attttctcga
     2701 tatagcaaac atacttgccc atcagaatat ttaatgacaa taatttcatc tgacgctgcc
     2761 tgaatagttg caattttatt agaaaagtta gctcgtcctt ttctttgtaa ggataaactt
     2821 ctgaaatttt cattgatgtt aaaagtagat agtccgtaac tcatttaata tgctccatat
     2881 acaatctgaa tataatctga atcatcagcc ggtagactaa aaggaaaaat acaaacaact
     2941 tctcctgaaa tcgttttatc gttgatcgaa acattttcta aatgaataag attgggaata
     3001 ttcgttttat aacc
//
LOCUS       DANLRK010000225         2943 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.226, whole genome shotgun sequence.
ACCESSION   DANLRK010000225 DANLRK010000000
VERSION     DANLRK010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2943
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.226"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            245..1363
                     /locus_tag="PXA33_002438"
     CDS             245..1363
                     /locus_tag="PXA33_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HDL5121729.1"
                     /translation="MKKTLVALAVTAFAASASATTVYDAEGSKVDFDGSLRIVLDRTN
                     SKELDNHNVVKTKKDAHTGIRNTGSRFGVRVKHDLAQDFYALGRVEFRFNKNTSDDGF
                     GDLYTKRAYVGLGSKQYGELTFGRQVTIADDLSQSVDYNYGINPKGDYIQTDGNQVIR
                     YNYRGVENLHVSANYNFAQDRKDGKVLANKLQNAYGIGALYTVDNWDLRAAYGHTNYK
                     TATDSTKHRKDGVLGSVGYKFGDLKLSVDTGYSREKGLAVTDDNGVTTFYNGVKSNAF
                     YVSPNFEYQVVPASKVYGNYLYERVKSKSNGSTYAAGKEKTHGFLLGVDYQFHKQVVA
                     FVEGSYTRTKDYDKVTNGYEYSGKSTNKAIGVGMRVYW"
     gene            complement(1516..2880)
                     /gene="glmU"
                     /locus_tag="PXA33_002439"
     CDS             complement(1516..2880)
                     /gene="glmU"
                     /locus_tag="PXA33_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HDL5121730.1"
                     /translation="MTQLSVVILAAGKGTRMYSDLPKVLHPVAGKPMVKHVIDTAKQL
                     DAQQIHLIYGHGADLLKARLSEEPVNWVFQAEQLGTGHAMQQAAPFFADDENIVMLYG
                     DAPLITKATLERLIAAKPENGIALLTVELENPTGYGRIIRENGSVVAIVEQKDANEAQ
                     LKVKEVNTGVMVASGASFKKWLKNLNNNNAQGEYYITDVIAMANQEGFKVQAVQATEF
                     MEVEGANNRLQLAALERFYQKREAEKLLLAGVTIIDPSRFDLRGTVTHGKDVQIDVNV
                     ILEGEIKLGNNVKIGAGCVLKNCEIGDNVEIKPYSVIEDSIVGAKSAIGPFSRLRPGA
                     ELAEETHIGNFVEIKKATIGKGSKVNHLTYVGDAEIGKECNIGAGVITCNYDGANKFK
                     TIIGDNVFVGSDSQLIAPVTIASGSTIGAGATVTKDIAENELVISRVPQRHIQGWQRP
                     TKKK"
BASE COUNT          852 a          533 c          578 g          980 t
ORIGIN      
        1 ctgttaaaaa tgagtggatt ttctccaaat gaaataatgt aagtataacc atttgatttt
       61 aaaagaaaaa atgccgtctt tgttttgggg tttgaactat tgacattgag ttttcttttt
      121 gcaattattc tggcgaaaac aatgttaggc gttttgctga tacattgtat cttggataat
      181 tactcttaca ccttaatgag ggtaattact tatcatataa catataaaat ctaaggtgaa
      241 aattatgaaa aaaacattag ttgcattagc agtaaccgcg ttcgcagcgt cagcatcagc
      301 aaccaccgtt tacgatgcag aaggttcaaa agtagatttt gatggttctt tacgtattgt
      361 tcttgataga acaaattcta aagagctaga caatcataat gttgttaaaa ctaaaaaaga
      421 tgcacacaca ggaatccgta acactggttc acgttttggt gtaagagtaa aacatgattt
      481 agctcaagat ttctatgcat taggtcgtgt tgagttccgt tttaacaaaa ataccagtga
      541 tgatggtttt ggtgatttat atactaaacg tgcttatgtt ggtttaggta gtaagcaata
      601 tggtgaatta acttttggtc gtcaagtaac gattgctgat gatttaagtc aatcagtaga
      661 ttacaactac ggtattaatc caaaaggtga ttatattcaa accgatggta accaagttat
      721 tcgttataac taccgtggtg ttgaaaactt acatgtaagt gcaaactata actttgcaca
      781 agatcgtaaa gatggtaaag tattagcaaa taaacttcaa aatgcatacg gcattggtgc
      841 attatacact gtagataact gggatttacg tgcagcttat ggtcatacaa actacaaaac
      901 agctactgat agcactaagc accgtaaaga tggtgtatta ggttctgttg gttataaatt
      961 tggtgactta aaattaagtg ttgatacagg ttattctcgt gagaagggtt tagctgttac
     1021 ggatgataat ggtgtaacta cattctataa tggtgttaaa tcaaacgctt tctatgtatc
     1081 tccaaacttt gaatatcaag tagtgcctgc atctaaagta tatggtaact acttatatga
     1141 gcgtgtgaaa tctaaatcaa atggttctac atatgctgct ggtaaagaaa aaactcatgg
     1201 tttcttactt ggtgttgatt accaattcca taaacaagta gttgcttttg ttgagggttc
     1261 ttatactcgt actaaagact atgataaagt aactaatggt tatgaatact caggcaaatc
     1321 aactaacaaa gctatcggtg ttggtatgcg tgtatactgg taatcaattg attttctaaa
     1381 ttgataaacc cctaacaata tttattgtta ggggttttgt tttaaatata ttgttcatta
     1441 tcctttttaa agtgtatggc tttgctgtta ttggcaaagc caatttttaa atataataac
     1501 ttttgaccac tggcactatt tctttttagt cgggcgttgc cagccttgaa tatggcgttg
     1561 tggtacacgg ctaattacca gttcattttc cgcaatgtct ttagtgactg ttgcaccggc
     1621 accaatggtt gaaccgctgg caatcgtgac cggggcaatg agttggctgt ctgagccgac
     1681 aaatacgtta tcgccaataa tggttttgaa tttattcgca ccatcatagt tacaagtgat
     1741 tacacctgct ccgatgttac attctttgcc gatttccgca tcgcctacat aagtgagatg
     1801 atttactttt gaacctttgc cgatggtagc ttttttgatt tccacaaagt taccgatatg
     1861 ggtttcttcc gctaattctg cccccgggcg taagcgagag aatggaccga tagcggattt
     1921 tgccccgaca atcgaatctt caatcacaga ataaggttta atttcaacat tatcgccaat
     1981 ttcgcagttt ttcagtacgc agcctgcacc aatttttaca ttgttaccga gtttgatttc
     2041 accttctaga atgacattca catcgatctg aacatctttt ccgtgggtga cagtgccacg
     2101 aagatcaaaa cggcttgggt cgataatagt tacaccagcc aataacagtt tttccgcctc
     2161 acgtttttgg tagaaccgtt caagtgcagc aagttgcaaa cggttgtttg ccccttctac
     2221 ttccataaat tcagttgcct gaaccgcttg tactttaaag ccttcttggt ttgccattgc
     2281 gatcacatca gtgatgtaat attcaccttg agcattgttg ttatttaggt ttttcaacca
     2341 ttttttgaag ctcgcaccgc ttgctaccat cacgccggta ttcacttctt tgactttaag
     2401 ctgtgcttca ttggcatctt tttgctcaac aatcgctacc acagaaccat tttcacgaat
     2461 aattcgtccg tagcctgtcg ggttttccaa ttcaacggtt agtaatgcaa taccattttc
     2521 cggtttggct gcgattaaac gctctaaagt ggcttttgtg attagggggg catcaccata
     2581 aagcattaca atgttttcat catcagcgaa aaaaggagct gcttgttgca ttgcatgtcc
     2641 tgtgcctaat tgttcggctt ggaataccca gtttacaggc tcttcgctta aacgtgcttt
     2701 taacagatcg gcaccgtgtc cgtaaattaa atgaatttgc tgggcatcta gctgtttggc
     2761 agtatcaata acgtgtttta ccatgggttt accggcaact gggtgtaaca ctttgggtaa
     2821 atctgaatac atacgagtgc ctttaccggc agctaaaata acaacgctca gttgagtcat
     2881 atagaatcct tatttgacta aatagatgaa ggcttatagt aacgtgtaaa ttgctataag
     2941 tga
//
LOCUS       DANLRK010000226         2910 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.227, whole genome shotgun sequence.
ACCESSION   DANLRK010000226 DANLRK010000000
VERSION     DANLRK010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2910
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.227"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            26..823
                     /locus_tag="PXA33_002440"
     CDS             26..823
                     /locus_tag="PXA33_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_157140410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL5121731.1"
                     /translation="MRYGDNTTFKRSCVIQKRVIHALLMRELITRYGRKNIGFLWLFI
                     EPLLLTLVITVAWKYIKADKFSTLNIIAFMITGYPIAMMWRNASNRAQGAISANLSLL
                     YHRNVRVLDTIFTRVLLEVTGASIAQILLMTIFIAIGWIEMPNDIFYMLCAWILMAFF
                     ALGLGLIICTLAHYIEPFGKIWGTFSFFLLPLSGAFFFVHNLPLTAQNIALWFPMIHG
                     TEMFRHGYFGETVITYESISYLIVSDIILLFIGLLCVRSFSKGIEPR"
     gene            820..1467
                     /locus_tag="PXA33_002441"
     CDS             820..1467
                     /locus_tag="PXA33_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015702020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL5121732.1"
                     /translation="MISIRNVSKQYRTNNGWKTILKDINFELKKGEKVGILGRNGAGK
                     STLIRLMSGVEPPTTGTIERQMSISWPLAFSGAFQGSLTGMDNLRFVSRIYDADFDYV
                     KSFTEEFSELGEYLYEPVKSYSSGMKARLAFALSLSIEFDCYLIDEVIAVGDARFAAK
                     CKHELFEKRKDRSLILVSHSPASIKEYCDSAMVLEEGIMHTFDSIDNAYNFYNKT"
     gene            complement(1545..2174)
                     /locus_tag="PXA33_002442"
     CDS             complement(1545..2174)
                     /locus_tag="PXA33_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HDL5121733.1"
                     /translation="MDFSKILSQVLDTAKDAATNGLIKGNSKNDQIAKIGGGAVAIGL
                     ISMLFGRNGGSSLAKLGSLAAIGSLAYQAYQKYQAQNSSVSELSQNNFENVDENVASK
                     VILQAMIAAAASDGAITEEEKAAIVSQVGDEAEVIEWIRQEMDTPATVEEIARQVGND
                     QALATQVYLASRAVCRDLERKEIVFLANLADALGLAEALVEQLEKDAGF"
     gene            complement(2246..2719)
                     /locus_tag="PXA33_002443"
     CDS             complement(2246..2719)
                     /locus_tag="PXA33_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HDL5121734.1"
                     /translation="MLEKITACPCQTGKRYAECCQPFHLKQTFASTAEQLMRSRYSAY
                     TLVNIPYIVETTVPSQQKLLDQTAMKEWAENTKWVGLSVLNHLPSLSRIHSQVEFKAF
                     FDTDEGIQGHHETSLFVQIDERWFFVDPTVPLPHQKQPCVCGSGKKFKHCCGNYL"
BASE COUNT          897 a          515 c          532 g          966 t
ORIGIN      
        1 caagtattag agagcataaa aactaatgcg atatggtgat aatacaacct ttaaacgttc
       61 ctgtgtgatt caaaaacggg tgattcacgc ccttttaatg agggaattaa ttactcgtta
      121 tgggcgtaaa aatattggct tcttatggct ttttatcgaa ccattattgt taacgttagt
      181 gattactgtt gcttggaaat atataaaagc agataagttt tcaactttaa atattatcgc
      241 ttttatgatt acaggctacc ctatcgcaat gatgtggaga aatgcctcta atagagctca
      301 aggagcaata tcagcaaact taagtttgct ttaccacaga aatgtgagag tgctagatac
      361 gatatttact cgagttttat tagaagttac tggcgcatct atcgcccaaa ttctcttaat
      421 gactatattt atcgctattg gttggataga aatgcctaac gatattttct atatgctctg
      481 tgcttggata ttaatggcat tttttgcctt aggtttaggt ttaattatct gtactttagc
      541 acattatatt gaaccttttg gtaagatttg gggaacattc agcttctttc tacttcctct
      601 ttctggtgca ttcttctttg tccataattt accgctaact gctcaaaata ttgccttatg
      661 gtttccaatg attcatggta cagaaatgtt tagacatggt tattttggtg aaacggtaat
      721 tacctatgag agcattagtt atcttatagt aagtgatatt attcttctat ttataggact
      781 gctttgtgtg agaagtttta gtaaagggat agaaccaaga tgattagtat tagaaatgta
      841 agtaagcaat atcgaactaa taacggttgg aaaaccatat taaaagatat aaattttgag
      901 ctgaaaaaag gcgaaaaagt ggggatttta gggcgcaatg gtgcaggaaa atcaacctta
      961 atccgtttaa tgagcggagt agaacctcca acaacaggta cgattgaacg tcaaatgtcg
     1021 atttcttggc cactggcatt tagtggtgca ttccagggta gtttgaccgg aatggataat
     1081 cttcgtttcg tcagccgaat ttatgatgct gattttgact atgtaaaatc tttcaccgaa
     1141 gaattttcag aactcggtga atatttatat gaaccggtaa aaagctattc ttctggtatg
     1201 aaggcccgtt tagcctttgc attatcgctt tctattgaat ttgattgtta cttaattgat
     1261 gaagtgattg cagtaggcga tgctcgattc gctgccaaat gtaagcacga gctttttgaa
     1321 aaacggaaag accgttcatt gattttagtt tctcatagcc cagcctcaat taaagaatat
     1381 tgcgattctg caatggtttt agaagagggt atcatgcata cttttgattc tatagataac
     1441 gcttataatt tctacaataa aacataaaag aacaagcggt tatatttcac aagtttttcg
     1501 caaaaaactc tcaaaatata accgcttgtt actaaaactg aaaattagaa acctgcgtct
     1561 ttttctaatt gttcaactaa tgcctcagct aaacctaaag catcagctaa attagctaag
     1621 aaaacgattt ctttgcgttc taaatctctg catactgcac gggaagctaa gtaaacttgt
     1681 gtagccaatg cttggtcatt acctacttgg cgggcaatct cttcaactgt agccggagtg
     1741 tccatttctt gacgaatcca ctcaatgact tcagcctcat ctccaacttg ggaaacaata
     1801 gctgcttttt cctcttcagt aatagcacca tctgaagcag cagcggcaat cattgcttgt
     1861 aaaatcactt tacttgccac attttcatct acattttcaa aattattttg gctaagttct
     1921 gaaaccgatg aattttgcgc ttgatacttt tggtatgctt gataagcaag gctaccgata
     1981 gcggctaaag agcctaattt tgctaaactt gaaccgccat tacgcccaaa taacattgaa
     2041 attaaaccaa tagcgacagc gccgccacca attttagcga tttgatcgtt tttagaatta
     2101 cctttaatta atccgtttgt agcggcatct tttgctgtat ctaatacttg acttaaaatc
     2161 ttactgaaat ccatttttgt tcctcttggt aagtaaatga aatttactca ttagtcctta
     2221 gattcaaaca agagttcaat tcaaattaaa gataatttcc acaacagtgc ttaaacttct
     2281 ttccggagcc acatacacaa ggttgttttt gatgaggcaa aggtacggta ggatccacaa
     2341 aaaaccatcg ctcatcaatt tgaacaaaaa gagaggtttc gtggtgtccc tgtatcccct
     2401 catctgtatc aaaaaaggct ttaaattcaa cctgactatg tattctcgaa agagaaggta
     2461 agtgatttag cacggataat cctacccatt ttgtattttc agcccactct ttcattgcag
     2521 tctgatcaag taatttctgc tgacttggca ctgtcgtttc cacaatatag ggaatattaa
     2581 ccaaagtata agcactgtaa cgggagcgca ttaattgctc tgccgttgat gcaaatgtct
     2641 gttttaaatg gaaaggttgg caacattcag cgtagcgttt gcctgtttgg caaggacaag
     2701 cggtgatttt ttctaacatt tttacatttc cgcataaaaa taaagatgtg aaaagtttac
     2761 gctataaata aagaaaaata aattttataa aaaagcgtga catacccctt gtggataact
     2821 tatgcaaaaa tcgtgaattt tttgggtatt cattgcttta aaaagccttt attctataga
     2881 ttgtggataa cttaagattt tatccacagg
//
LOCUS       DANLRK010000227         2867 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.228, whole genome shotgun sequence.
ACCESSION   DANLRK010000227 DANLRK010000000
VERSION     DANLRK010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2867
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.228"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            <1..467
                     /locus_tag="PXA33_002444"
     CDS             <1..467
                     /locus_tag="PXA33_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121735.1"
                     /translation="GELHDYEFEHQQVAELNNFLKSLIANKIKSVEANFEVKESKKVE
                     GSYRIILDGKSLTESERSTVMDTFHFNSAQNPSITYQAEGNLVKLANREELLQKFRFK
                     QALEANVRYYKMKSGLQNTAEGALSIVMLPVAVVAVLPFYMFWVGACATYGC"
     gene            478..963
                     /locus_tag="PXA33_002445"
     CDS             478..963
                     /locus_tag="PXA33_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HDL5121736.1"
                     /translation="MYSLYLKILSECDWCCDPKTLSTYQQEWLLHEGSLTQKLYDVAT
                     NFNVEVVQEKWIAKNSEKTTACNEDVWSREVLLKDGEKNWIFAQTLVPRSTIENIAQN
                     VLELGNQPIGLWLFSQNPTRTKLEWTQTENGLFARRGVYEIRGYSLEIRELFLETFPY
                     L"
     gene            complement(1001..1870)
                     /locus_tag="PXA33_002446"
     CDS             complement(1001..1870)
                     /locus_tag="PXA33_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDL5121737.1"
                     /translation="MKNINTQAVGWIGLGQMGEPMAKHLLNSGVNVGVYNRDPAKAEK
                     VVEAGGTLYPSVLELVKAYDVIFLMIADYAAVQQVLSDEVLAELNGKVVVNMSTISPS
                     QNQAVEALLAQHGAEFVEAPVSGSSKVAEAGKLLVLAAGKEETVELLKPLFAAFSTTT
                     FYYGEVGKAGGIKLMINSLLGIFIQAYGEALNFADQYGIPKEKVIEMISGSFMNSPIF
                     QAKVPMYQANEFPPAFMMKHMTKDFNLASEEIAKLGKSYPLIEQATKTYNQANDSGLS
                     EVDMAAIYQFLAK"
     gene            2000..2791
                     /locus_tag="PXA33_002447"
     CDS             2000..2791
                     /locus_tag="PXA33_002447"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HDL5121738.1"
                     /translation="MKPTILLYDSGMGGLTIYDEIRKVLPHAHYLYCFDNAFFPYSEK
                     SEAVLIERAVKIVQKIAEKQPLDMVVVACNTASTVVLPALREHFNFPIVGTVPAIKPA
                     AAISETKVIGLLATKGTVARPYVDELIRKYAQDCTVEKIGTTDLVEIVEEKQRSGKVD
                     MNRLQKVVEAWQGKPELDTVILGCTHFPLVKAELQQLLPDVKYFVDPGVGIAKRVLSL
                     LEERKFQQKADFENQAFCTKQSSAFNIKQDVMQNWGFNNLSVIDI"
BASE COUNT          870 a          544 c          599 g          854 t
ORIGIN      
        1 tgggtgaact gcacgattat gaatttgagc atcagcaagt tgcggaactg aataattttc
       61 tcaaaagcct aattgctaat aaaatcaaat ctgttgaggc aaacttcgag gttaaggaat
      121 ccaaaaaagt ggaaggtagt tatagaatta ttttagatgg gaaatcactt acagaaagtg
      181 aaagaagtac ggtgatggat acctttcatt ttaattcagc tcaaaatcca tctattactt
      241 atcaggcgga agggaacttg gtgaaattag ccaatagaga agagcttttg caaaaattcc
      301 gatttaaaca agcgcttgag gcgaatgtgc gttattacaa aatgaaaagt ggtcttcaaa
      361 atacggctga aggagcgtta agtattgtaa tgttgcctgt tgctgtagta gctgttttac
      421 cattctatat gttttgggtt ggtgcttgtg ccacttacgg atgttaaata actttttatg
      481 tattctctat acctgaaaat tttatcggaa tgcgattggt gttgtgatcc aaaaacactt
      541 tctacctacc agcaagaatg gcttctgcac gaagggtcac ttacccaaaa attgtatgat
      601 gtcgcaacca atttcaacgt ggaagtagta caagaaaagt ggattgcaaa aaacagtgaa
      661 aaaacgaccg cttgtaatga agatgtttgg tcgcgagaag tgttactgaa agatggtgaa
      721 aaaaattgga tttttgccca aactcttgtg ccaagatcaa caatcgaaaa tatcgctcaa
      781 aacgtgttgg aattgggcaa tcagccgatt ggtttatggc tattttctca aaatcctact
      841 cgcaccaaat tagaatggac acaaacagag aacggtttat tcgctcggcg tggggtgtat
      901 gaaattagag ggtattctct ggaaattcga gagctttttt tagaaacctt tccttattta
      961 taaaaaaatc ggttggtaat gacaccaacc gattttcatc ttattttgcc aagaactgat
     1021 aaattgcagc catatccact tcgctcaagc ctgaatcatt ggcttgattg taggttttag
     1081 tggcttgctc aataagcggg taagatttgc ctaatttcgc aatttcttcg cttgccaaat
     1141 taaaatcttt ggtcatatgt ttcatcataa acgcaggcgg aaattcatta gcttggtaca
     1201 tcggcacttt tgcttggaag atcgggctgt tcataaacga gcctgaaatc atttcgatta
     1261 ctttctcttt cggaatgcca tattggtcgg cgaagtttaa cgcctcgcca taggcttgaa
     1321 tgaagatgcc cagtagcgag ttgatcatca atttaatccc accggcttta cccacttcac
     1381 cataatagaa agtggtggta ctaaaggcag cgaaaagcgg tttgagcaac tcaacggttt
     1441 cttctttacc ggcagccagt accaatagtt tacctgcttc tgccactttg cttgaaccgg
     1501 aaaccggagc ttcaacaaat tctgcaccgt gttgggctag tagagcttct accgcctggt
     1561 tttggcttgg ggaaatggtg ctcatattca ccactacttt gccgtttaat tcagccaaca
     1621 cttcatcgct taacacctgt tgcacggcgg catagtctgc aatcatcaag aaaatcacat
     1681 cgtaggcttt caccaattcc aacacagaag gatataatgt gccgcctgct tcaactacct
     1741 tttctgcttt tgctgggtcg cggttgtaaa cgcccacgtt cactccgcta tttagcaaat
     1801 gttttgccat cggttcgccc atttgcccta aaccaatcca accgactgct tgtgtattaa
     1861 tgtttttcat tatttttctc cttactgaat aaagcaaatt gattattgcg attttccctc
     1921 atcaagtcaa atagagagaa cttgaatata gcaatggaaa agagtagcat tttagcactt
     1981 acttttttga acaataccaa tgaaaccaac aattctttta tacgattccg gaatgggcgg
     2041 attgacgatt tacgatgaaa tccgaaaagt gttaccgcac gctcactatt tatattgctt
     2101 tgataacgct tttttcccat attccgagaa gtcggaagca gttttaattg agcgagcggt
     2161 caaaattgtg caaaaaattg cagaaaaaca accgcttgat atggttgttg ttgcttgtaa
     2221 tacggcaagc acagtggttc tgccggcatt gcgtgagcat tttaattttc cgatagtagg
     2281 aactgtaccg gcaattaaac cggcagcggc aatttccgaa actaaagtca tcggcttgct
     2341 cgccactaaa ggcacggttg ctcgccctta tgttgatgaa ttgataagaa aatatgctca
     2401 ggattgtacg gtagaaaaaa ttggcacaac cgatttggtt gaaattgttg aagaaaaaca
     2461 acgaagcggt aaagtggata tgaaccgact acaaaaagtg gtcgaggctt ggcaaggtaa
     2521 gccggaattg gatacggtta tactcggttg tacccatttt cccttagtca aagctgaatt
     2581 acagcaatta ttgcccgatg tgaaatattt tgttgatccg ggtgtaggta ttgccaaacg
     2641 ggtgctaagc cttttggaag aacgcaaatt tcaacaaaaa gccgattttg aaaatcaagc
     2701 attctgcact aaacaaagtt ctgcatttaa tatcaaacaa gacgttatgc agaattgggg
     2761 atttaataat ctttcggtga ttgatattta aacaaaacaa tctgttttga tgaaaaaagg
     2821 ttcagttgag caaaaacttt aaaaaagggc ttgcactcaa ttttaaa
//
LOCUS       DANLRK010000228         2847 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.229, whole genome shotgun sequence.
ACCESSION   DANLRK010000228 DANLRK010000000
VERSION     DANLRK010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2847
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.229"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(<1..671)
                     /locus_tag="PXA33_002448"
     CDS             complement(<1..671)
                     /locus_tag="PXA33_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraI domain-containing protein"
                     /protein_id="HDL5121739.1"
                     /translation="MRSMFKKFFGKNKSQSAVKSDTIFSIEQDNEGWLYPEKASQLLN
                     TELRQKYLGLLWQQVSMNRQMFEVLYQQPIERYAEMVQLLPASESHHHSHIGGMLDHG
                     LEVIAFSAKVRQSYILPPNVAPEEQSKQRDAWTAAAIYLALVHDIGKVVVDIEITLKD
                     GSRWFAWNGQPPQPYKFKYIKGRDYHLHPVLGGFLANQLIPKSAFDWLGQYPEVISSL
                     MYAMSG"
     gene            complement(775..1239)
                     /locus_tag="PXA33_002449"
     CDS             complement(775..1239)
                     /locus_tag="PXA33_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC family protein"
                     /protein_id="HDL5121740.1"
                     /translation="MMNTQQPKFDLYQHITDRIIAALEQGTAPWLKPWSHPECNMALP
                     RNAVSNRLYSGINTLLLWIATAEHGYKQCKWITAKKANELGGYIRKGEKATVVMNYRP
                     NEREKLDEEGNIVYDEEGNPEMEQHFFIAKHNLFNLDQCENLPAELYDPVIP"
     gene            1448..1711
                     /locus_tag="PXA33_002450"
     CDS             1448..1711
                     /locus_tag="PXA33_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme-like superfamily protein"
                     /protein_id="HDL5121741.1"
                     /translation="MRPVTRNNRGTSFDPRHNKYINTLATALLARWHIEQFSERNIPV
                     NDENLYMAHNIGLDGFHRALLGQSTRKDIENMRKNGMKPWMSI"
     gene            complement(1795..2820)
                     /locus_tag="PXA33_002451"
     CDS             complement(1795..2820)
                     /locus_tag="PXA33_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03752 family integrating conjugative element
                     protein"
                     /protein_id="HDL5121742.1"
                     /translation="MTAGDTPHDTLKTLVGALKDTREEARKANERGDRFEQEVLRLQA
                     REAEVDSRIAGEVESRVESRLAAAWDGFNRQIEELKGRVQSGNQQQKEAEAIPVGGAS
                     SGFGTESAGQTGNVDNNGIRWVDPDDMVVTDEQGKPVTGNYSGKTKTGFPSPFKAEGE
                     SSNTGTALNNNAGTENMTGNTQIQQIKRTPYYTIPENSTLMGSTAMTALLGRVPIQGA
                     VTDPFPFKVLIGRENLIANGIDLPDVEGAIVSGTSTGDWTLSCVRSEVTSITFVFADG
                     RISNGRASGESRAVKRNQASNLTPFCLTVSLSGFNDIPHSDGNPGFLTMSAVDYVDVY
                     LMSHLAR"
BASE COUNT          794 a          640 c          555 g          858 t
ORIGIN      
        1 ccagacattg catacatcag tgaagaaatg acttctggat actgtcccag ccaatcaaac
       61 gcagacttgg gaatgagctg atttgcaagg aatcctccta gcacgggatg aagatggtag
      121 tctcgacctt tgatgtattt gaatttataa ggttgaggtg gctgtccatt ccaagcaaac
      181 caacgagagc cgtctttaag agtgatttca atatcaacca caactttgcc aatatcatga
      241 accaaggcaa gataaattgc ggcagcagtc caagcatctc gttgctttga ttgttcttcg
      301 ggagctacat tcgggggcaa aatatagctt tgacgaactt tagctgaaaa agcaataact
      361 tctaaaccgt gatcaagcat tccaccgata tgtgaatgat ggtgtgattc agaagctggt
      421 aatagttgaa ccatctcagc gtaacgttct attggctgct gataaagtac ctcaaacatt
      481 tgacggttca ttgatacctg ttgccaaagt agcccaagat atttttgtcg taattctgta
      541 ttaagtaatt gagaggcttt ttccgggtat aaccaccctt cattatcttg ctcaatagaa
      601 aaaatagtat cggatttgac cgcactttga gatttatttt taccaaaaaa ttttttgaac
      661 attgaacgca ttggaagaga ccgaaaatat gaatgctgta ttgaatgaaa gccgtttagc
      721 cctttgccct tttgggtaag acctttcccc ttggaaaggt tactcttatc ccccttaggg
      781 gattacagga tcgtacagtt ccgccggtaa attttcacac tggtctaaat tgaacagatt
      841 gtgcttagca atgaaaaagt gttgttccat ttccggattt ccttcctcgt catacacaat
      901 atttccctcc tcatcgagtt tttctctctc attaggacgg tagttcatca caacagtagc
      961 tttttcccct ttacggatat atccgccgag ttcattggct tttttcgccg taatccactt
     1021 acactgtttg tagccgtgtt cagcggtagc aatccaaagc aataacgtat tgataccgga
     1081 atacagacgg ttagaaaccg cattgcgagg taatgccata ttgcattccg gatggctcca
     1141 cggttttaac cacggagctg taccttgttc caatgcagca atgattctat cagtaatatg
     1201 ttggtataaa tcaaatttag gttgttgagt gttcatcatg tgtttctcca aagtttgggt
     1261 aaaaaaatgg ggaaacacgg ttaccctatg gggaacttgt ttccccgatg ggtagtttat
     1321 aaaatttcaa tggccacaat ttttctagaa tcatcaaagc caacagaact gcctatggcg
     1381 tagtaattac gaatagtctg ttcatcggca ttgattagca gatacctatc gaggaagaga
     1441 gattgggatg agaccggtaa cgagaaataa tcgtggaacc agttttgacc ctcgtcacaa
     1501 taaatacatc aatacattgg caaccgcatt attggcacgt tggcatattg agcaattctc
     1561 tgagcgtaat atcccagtca atgatgaaaa tctctatatg gctcacaata ttggcttaga
     1621 tggtttccat cgagcgttat tagggcaatc tactcgtaaa gacattgaga atatgcgtaa
     1681 gaatgggatg aaaccttgga tgtccattta gctctgtttt caaatgacct aatccagttt
     1741 gttccggaga gagtaccgaa attttgcact ctttcatccg tggtgtatca aacttcaacg
     1801 tgctaaatga gacattaaat atacatcaac ataatctacc gcactcatcg ttaagaaacc
     1861 gggattccca tcagaatgag gaatatcgtt aaagcccgat aaggagacgg ttaaacaaaa
     1921 tggtgttaaa ttacttgctt gatttcgttt cacagctctg ctttctccac tcgctcgccc
     1981 attcgaaata cgaccgtctg caaacacaaa ggtaatgctc gttacctcag aacgaacgca
     2041 agatagcgtc caatctccgg ttgatgtacc ggatacaatc gccccctcaa catcaggtaa
     2101 atcaatgcca ttggcaatta aattttctcg cccgattaat actttaaacg gaaacggatc
     2161 tgtcacagca ccttgaatag gtacacggcc taacaatgcc gtcatcgcag tagaacccat
     2221 taaagtcgaa ttttcgggaa tagtgtaata aggtgtacgc tttatttgct gtatctgagt
     2281 attaccagtc atattttctg tcccagcatt attatttaat gctgtcccag tatttgaact
     2341 ttccccctct gctttaaaag ggcttggaaa gccggttttt gttttcccgg aataattgcc
     2401 tgtaaccggt ttaccttgtt catcggttac caccatatca tcaggatcga cccagcgaat
     2461 gccattgtta tctacattcc ctgtttgtcc tgctgattca gtcccaaagc cagaagaagc
     2521 ccctcctaca gggattgcct ctgcttcttt ctgctgctga ttaccacttt gaacccgacc
     2581 tttcagttct tcgatttggc gattaaatcc atcccaagca gcagccaaac ggctttctac
     2641 acgactttct acttcacccg caatacgaga atccacttcg gcttctcttg cttgtaagcg
     2701 taatacctct tgctcaaagc gatcaccacg ctcatttgct ttacgagctt cttcacgagt
     2761 atcttttaat gctcctacca acgtttttaa tgtatcgtga ggcgtatctc ctgcagtaat
     2821 acctaatgta ttcgcctcct catcact
//
LOCUS       DANLRK010000229         2753 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.230, whole genome shotgun sequence.
ACCESSION   DANLRK010000229 DANLRK010000000
VERSION     DANLRK010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.230"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            23..1090
                     /gene="hisC"
                     /locus_tag="PXA33_002452"
     CDS             23..1090
                     /gene="hisC"
                     /locus_tag="PXA33_002452"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HDL5121743.1"
                     /translation="MTISQLSRKNIQALTPYQSARRLGGSGDVWLNANEYALSPNFDL
                     TDRTFNRYPEPQPQAVIEGYARYAGVAPENVLVSRGGDESIELIIRAFCEANDSILYC
                     PPTYGMYAVSADTCGIATKTVPLTADFQLDLAQIKANLEGVKVVFVCSPNNPTGNLIK
                     RSDLFELLQITAGKAIVVVDEAYIEFCPEARLVSELANFPHLAIIRTLSKAFALAGLR
                     CGFTLANAELIGVLQKVIAPYPLPVPVSDIAAQALSPQGIEQMKTRVAEVITLRAELQ
                     KNLENLPLVEQVFDSEANYLLFKCQDGQKVFKALWEQGIILRDQHKALGLQNCIRITV
                     GAREENLRVVEAITALSQFQR"
     gene            1275..2363
                     /gene="hisB"
                     /locus_tag="PXA33_002453"
     CDS             1275..2363
                     /gene="hisB"
                     /locus_tag="PXA33_002453"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018355873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HDL5121744.1"
                     /translation="MQPTLFIDRDGTLIDEPKTDFQIDSLEKLKFEKNVIPALLKLKG
                     KYRFVMVTNQDGLGTSSFPQADFDKPHNAMMALFNSQGIEFDDVLICPHKPEDGCDCR
                     KPHTKLLQKYIDRQLFDPANSFVIGDRATDVQLAENLGIRALQYHPEKLNWDLIAEKL
                     LGEAVTNIGERPKRFAEVIRKTKETDIKVQVWLDETGNNEISTGVGFFDHMLDQIATH
                     GGFRMNITTKGDLWIDEHHTVEDTALALGTALKQAIGDKRGIARFGFVLPMDESRAEC
                     AMDLSGRPFIKFKADFKRDKVGDFSTELTEHFFQSIAFTMLATLHIKAKGDNDHHKIE
                     SLFKVFGRTLRQCIRIEGNELPSSKGVL"
     gene            2458..2742
                     /locus_tag="PXA33_002454"
     CDS             2458..2742
                     /locus_tag="PXA33_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121745.1"
                     /translation="MIREVIDFIKNNKWYVLLVVLLLGLYFYQFHGGLSSDSEKWDHF
                     GSYIGGIFSAVTLLSVLHGMQLERKRFKEQKEEFENDKRVLEKRRRKEDF"
BASE COUNT          793 a          637 c          662 g          661 t
ORIGIN      
        1 ataaattata caggaacaca atatgacaat ttcacaactt tcccgtaaaa acattcaagc
       61 tctcacccct taccaatcgg cacggcgttt ggggggtagt ggcgatgtgt ggctgaatgc
      121 caatgaatat gccctttcgc caaactttga tttaaccgac cgcactttta accgctaccc
      181 tgaacctcaa ccgcaggcgg tgattgaagg ttacgcccga tatgcaggcg ttgcacctga
      241 aaacgtgctg gtttcccgtg ggggcgatga gagcattgag ctgattatcc gagccttttg
      301 cgaggcaaac gacagcattc tctactgccc accaacctac ggaatgtacg ccgtgagtgc
      361 cgacacctgt ggcattgcga ccaaaactgt gcctttaacc gccgatttcc agttggattt
      421 agcccaaatc aaagctaacc tcgagggcgt gaaagtggtg tttgtgtgca gcccgaacaa
      481 cccgacaggc aacttaatca agcggtcgga tttgttcgaa cttttgcaaa tcaccgcagg
      541 caaagcgatt gtggtggtgg acgaagccta catcgaattt tgccctgaag ctcgcttggt
      601 aagcgagctg gcgaatttcc cacatttggc gattatccgc accctatcca aagcctttgc
      661 cttggcgggt ttgcgttgtg gctttacctt ggcaaacgct gagctgatcg gcgtgttaca
      721 aaaagtgatt gccccgtatc cgctgcctgt gcctgtttcc gacatcgccg cccaagccct
      781 ctccccacaa ggcattgagc agatgaaaac ccgtgtggcg gaagtcatca ccctgcgtgc
      841 cgaattgcaa aaaaatctgg aaaacttacc gcttgtagaa caagtttttg acagcgaagc
      901 caactacctc ctattcaaat gccaagacgg acaaaaagtc ttcaaagcac tttgggagca
      961 aggcattatt ttgcgtgacc aacacaaagc gttaggattg caaaattgca ttcggattac
     1021 ggtgggggcg agagaggaga atttgagggt ggttgaggcg attacagcac tgagccaatt
     1081 tcagagatag aatttaacga gcagtatttt aagagacatt tatagcctag cacggcatag
     1141 ccgtactagg ttacgcataa gtcagtccct ttgggactga atacagagag gcaaagtctc
     1201 tcactaatgc ttaaccccat atgccttggc gtgtggggag agtaatgaat aagatacaga
     1261 tacaggagaa accaatgcaa cccacccttt ttatcgaccg tgacggcacg ttaattgatg
     1321 agccaaaaac cgattttcaa atcgacagct tggaaaagct gaaattcgag aaaaacgtga
     1381 ttcctgccct gctcaaactc aagggcaaat accgctttgt gatggttacg aaccaagacg
     1441 gcttaggcac gagttcgttc ccacaggcag atttcgacaa accgcacaat gcaatgatgg
     1501 cgttgttcaa ctcgcaaggg attgaatttg atgatgtgct gatttgtcca cacaagcccg
     1561 aagacggttg cgattgccgc aaaccgcaca ccaaactgct acaaaaatat atcgaccgcc
     1621 aactgttcga cccagccaac agctttgtga ttggcgaccg tgctaccgat gtgcaactgg
     1681 cggaaaacct tggcattcgg gcgttgcaat atcaccctga aaaactgaac tgggatttga
     1741 ttgctgaaaa actactgggc gaagccgtca ccaacattgg cgagcgacca aagcgctttg
     1801 ccgaagttat ccgtaaaacc aaagagaccg acatcaaagt gcaagtgtgg ctggacgaaa
     1861 caggcaacaa cgaaatcagc accggcgtgg gctttttcga ccatatgctt gaccaaattg
     1921 ccacccacgg cggttttaga atgaacatca ccaccaaagg cgacttgtgg attgacgaac
     1981 atcacacggt ggaagacacc gccctcgccc tcggcaccgc attaaaacaa gcgattggcg
     2041 acaaacgcgg catcgcccgt ttcggcttcg tgttgccgat ggacgaaagc cgtgcggaat
     2101 gtgcgatgga cttatcaggc agacctttca tcaagttcaa agcggatttc aaacgcgaca
     2161 aagtgggcga tttcagcacc gagctcaccg aacacttctt ccaatccatc gccttcacga
     2221 tgctcgccac cctgcacatc aaggcaaaag gcgacaacga ccaccacaaa atcgaaagcc
     2281 tgttcaaagt cttcggcaga acactcagac agtgcattcg tattgaaggg aatgaactac
     2341 caagcagtaa aggtgtatta taatcaccgt aggggcgggt tttaacccgc ccgaaagtgc
     2401 ggttgaaatt tgcaaagttt ttggggattt tgaccgcttg ttataaagga tatattgatg
     2461 attagagaag ttattgactt tataaagaat aataaatggt atgttctgtt agttgtattg
     2521 ttattaggat tatattttta tcagtttcac gggggattat cctcagatag tgaaaaatgg
     2581 gatcattttg gtagctatat tggtggtata ttttctgctg ttactttatt gagtgttttg
     2641 catggaatgc agttggaacg taaaagattt aaggagcaaa aagaagaatt tgaaaatgac
     2701 aaaagagtat tagaaaagag aagaagaaaa gaagattttt agcgtacatt ttt
//
LOCUS       DANLRK010000230         2749 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.231, whole genome shotgun sequence.
ACCESSION   DANLRK010000230 DANLRK010000000
VERSION     DANLRK010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.231"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            22..834
                     /locus_tag="PXA33_002455"
     CDS             22..834
                     /locus_tag="PXA33_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL5121746.1"
                     /translation="MGRPELERAVLSVQNQTYPCKHYVFVDGEKYHQQAADILKNFPS
                     VIVTYLPMNTGADGWVNSSINAIAPFLVKEDIICYLDDDNWFEPTHVESGVHALLEMQ
                     SDYAYSLRNFYDLDGEFLCIDTIESLGEYNCVYQEPLECNYQLGGVKGVLSINLNDCQ
                     HIDTNCYFIRSEIARLISTAWYTGIHNDRNVYKKLQELALKGNCTKKVTVNYTLDTRK
                     YYGSVFNELLEEPFLLSEYDAYQFTNEVIKFHSEESLKAWGGCLIWEQNNGS"
     gene            966..1664
                     /gene="aphA"
                     /locus_tag="PXA33_002456"
     CDS             966..1664
                     /gene="aphA"
                     /locus_tag="PXA33_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HDL5121747.1"
                     /translation="MTAATMFASLIAFSAQAGGPKVAYTQPGIDMRTHEAQQAPIHWV
                     SIEQIKESIKDLPPMAVSFDIDDTVAATSGCFHYGKQKYSPDDFSYLKNQDFWDEINA
                     GCDKYSLPKQVAINLIKMHQERGDQVYLITGRTAGKNEQVTTIMQKYLGIKNMQPVNF
                     MGGKAHSTKYDKTPAIIEHKVQIHYGDSDDDILAAREAGIRGIRILRAANSNYTPFPQ
                     AGGYGEEVVVNSSY"
     gene            1845..2606
                     /gene="udp"
                     /locus_tag="PXA33_002457"
     CDS             1845..2606
                     /gene="udp"
                     /locus_tag="PXA33_002457"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HDL5121748.1"
                     /translation="MSEVFHLGLTKAMLKGAKIAIVPGDPARSERIAKKMDNPEFLAS
                     TREFTSWLGYLDGEPVVVCSTGIGGPSVSICVEELAQLGVRTFLRIGTTGAIQEHINV
                     GDILITTGAVRLDGASQHFAPMEYPAVANFECTNALYAAAVESGVTPYVGITASSDTF
                     YPGQERYDTFTGKIYRHFQGSLKQWQDLNVMNFEMESATLFTMCSALGLRAGMVAGVI
                     VNRTQQEIPNEATIHDTEHKAVSVVVEAARKLIKG"
BASE COUNT          870 a          442 c          572 g          865 t
ORIGIN      
        1 gtagctgtta tcagttcaac aattggtcgt cctgaattag aaagagctgt tttaagcgta
       61 caaaatcaga cttatccttg taaacattat gtttttgttg atggagagaa atatcaccaa
      121 caagcggctg atattttaaa gaattttcca agtgttattg ttacttatct cccaatgaat
      181 accggggctg atggttgggt aaatagcagc attaatgcaa ttgcaccatt tttagtgaaa
      241 gaagatatta tttgttatct ggatgatgat aattggtttg aacctactca cgttgaatct
      301 ggtgtgcatg ctttattaga aatgcaatct gattatgctt actctttacg taatttttat
      361 gatttagatg gcgagttttt gtgtattgat acgattgaat cgttgggaga atataattgc
      421 gtttatcaag aaccattaga atgtaattat caattaggtg gtgttaaagg tgttttatca
      481 ataaatctga atgattgtca gcatattgat acgaattgct attttattcg tagtgagata
      541 gctcgcctta tcagtacggc ttggtacaca ggtattcata atgatagaaa tgtatataaa
      601 aaattacaag aattggcact aaaaggaaat tgtacaaaga aagttacggt taattatact
      661 cttgatacac gtaaatatta tggatcagta tttaatgaat tattagagga gccattttta
      721 ttaagcgaat atgatgccta tcaatttacg aatgaggtga ttaaatttca ttcagaagaa
      781 agccttaaag cttggggagg ttgcttgatt tgggaacaga acaatggctc ataagcgtta
      841 agcgtaatcg tttgcttgtg atcatattca caaaataagg tttttgtatt agctaagagt
      901 gcctaataag attaaaatag gtatggttaa tcatatggag gtattttata aaatttacat
      961 taaaaatgac ggcagcgaca atgtttgcat ctcttattgc tttttcagca caagcaggcg
     1021 gaccaaaagt agcttatacc cagccgggaa ttgatatgcg aacacatgaa gcacagcaag
     1081 cgccaattca ttgggtatct attgagcaaa ttaaagaaag catcaaagat ttacccccta
     1141 tggcggtaag ttttgatatt gatgatactg ttgctgcaac tagcggttgc ttccactacg
     1201 gaaagcaaaa atattcgcca gacgatttca gctacttaaa aaatcaagat ttttgggacg
     1261 aaattaatgc tggctgtgat aaatattctt tgccaaagca agttgctatt aatttaataa
     1321 aaatgcacca agaacgtggt gatcaagttt atttaattac ggggcgtacc gcaggtaaaa
     1381 atgaacaagt tacaacgatt atgcaaaagt atcttggtat taaaaatatg caaccggtaa
     1441 attttatggg ggggaaagca cattcaacaa aatatgataa aacccctgcg attattgaac
     1501 ataaggtgca aattcactat ggtgacagtg atgatgatat cttagctgcc cgtgaagccg
     1561 gtattcgagg tattcgtata cttcgtgcag caaattctaa ttatacgcca ttccctcagg
     1621 ccggaggata tggtgaagaa gttgttgtca attcaagtta ttaatttctt agtctgaaat
     1681 atggccacca ttgcgtggcc atcatttttt atttatataa tgacgaaacc gtttaccttt
     1741 gatctagttc acaaaatcaa actttgcttg caaaaaattg tcgtaaatta accgcttgct
     1801 aagatagaat ggctcatatc cagttagata aaaaggagat aaaaatgtct gaagtatttc
     1861 atttaggttt aaccaaagcc atgctcaaag gtgcaaaaat tgcaatcgtt ccgggcgatc
     1921 cggctagaag tgaacgtatt gccaaaaaaa tggacaaccc agagttttta gcctcaaccc
     1981 gtgaatttac ctcttggtta ggttatttag atggcgaacc ggtggtggtt tgttcaaccg
     2041 gtattggcgg accgtcggtg tcgatttgtg tggaagagtt agcacagtta ggtgttcgca
     2101 cattcttacg cattggcaca acaggtgcaa ttcaagagca tattaatgta ggcgatattt
     2161 taattaccac cggagcggtg cgtttagatg gtgcgagcca acattttgca ccaatggaat
     2221 atcctgcagt agcgaatttt gaatgtacta atgctcttta tgcagcagca gtggaatcag
     2281 gcgttacacc ttatgtgggg attacagctt catcagacac tttctacccg gggcaagaac
     2341 gttacgacac tttcaccggc aagatctatc gtcatttcca aggttcatta aaacagtggc
     2401 aagatctcaa tgttatgaat tttgaaatgg aatccgccac tctctttacg atgtgttcgg
     2461 cattaggctt gcgagcaggt atggttgctg gtgtaattgt aaaccgcact caacaagaga
     2521 ttccgaatga agcaaccatt cacgacaccg aacataaagc cgtttcggta gtagtggaag
     2581 cggcaagaaa attaattaaa ggctaatctt taaatttaaa agcaaaaaag gtaaattgtc
     2641 tttgttacaa tttacctttt ttctttttag ggagctttct atgaaaaaat ggttgatgtt
     2701 aattacatta attttcggca ttaccgcttg tttttccagt ttagaattt
//
LOCUS       DANLRK010000231         2701 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.232, whole genome shotgun sequence.
ACCESSION   DANLRK010000231 DANLRK010000000
VERSION     DANLRK010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.232"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            <1..1333
                     /locus_tag="PXA33_002458"
     CDS             <1..1333
                     /locus_tag="PXA33_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDL5121749.1"
                     /translation="MNATSVSSIPKKESVTNVPTLDKKETEQNLTQLQKDFSAHQLEN
                     QKAKQSMINAQSELRRLNSQLNVLQKYVNSRRLGYYTQQAVLEQISIIQNKIKQTQTI
                     FNDANATVKLTDQKLEEAKLALGSVNDLVLIKASAPAMQATNQDTSMMNIIQADWISQ
                     YANTALSELSAQANSALQISNSLDRQLFKQSDKFNVWSSVEHQKTEHKSDLYRPYKQQ
                     TNLTQLGIQMPIDNGLMFGVALSKNHANAEFNEGVNGKSNLLMASLYGKWQSQQGTFI
                     SLDGSYGKAKNQLYLFGENHFTRRISSIGANIGHQFDLAGVQIQPTIGARYYHFSGQD
                     YTLGGAKISSPNTHFMTYQAGLKASKTFSLDDWKVEPSITTHYVDASNKRLAVNVNNN
                     NINQRFGRYLKTEAGIRFEHNQWQFDLHAGFINGNEIKKQHFAGVKLGYSW"
     gene            complement(1388..2482)
                     /gene="mobA"
                     /locus_tag="PXA33_002459"
     CDS             complement(1388..2482)
                     /gene="mobA"
                     /locus_tag="PXA33_002459"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HDL5121750.1"
                     /translation="MSKTIEERISAVILAGGLARRMNGVEKGLQLFEGKPLISHILAR
                     LSPQVSDIWLNVNRSIEQYQQLYPAFSYYQDSLPDFQGPLSGMLAGFEQIESDYLLFV
                     PCDTPFMPELLLQKLKTALRINNVQIAYAHDGERPHPTFALIHRSVQEDLKAYLGSNQ
                     QQLLAFFQSQKSVAVDFSEQKSAFTNVNTLEDLSRVSQFPVKNLAITGYSGTGKTTLL
                     EKLIPKLTACGIRVGLIKHSHHNVDVDKKGKDSYRLREAGANPTMIVCDERWALMTET
                     KQAVEFSQLIAKFNPQEVDLVFVEGFKHETLPKIQLHRKGIEKPLPELDQWTIATATD
                     YPLERENWLDINNIGGIADFIKNWLENKAS"
     gene            2569..>2701
                     /locus_tag="PXA33_002460"
     CDS             2569..>2701
                     /locus_tag="PXA33_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1040 family protein"
                     /protein_id="HDL5121751.1"
                     /translation="MKCERLEELLELLGEHWRKNPDLHLIDILQQLSVEVGEPDNFKA
                     "
BASE COUNT          883 a          556 c          495 g          767 t
ORIGIN      
        1 gatgaatgct acatctgtat cctctattcc aaaaaaggaa tctgttacta atgttcctac
       61 tttagataag aaagaaactg aacaaaatct tactcaacta caaaaagatt tttcagcaca
      121 ccaattagaa aatcaaaaag caaaacaatc tatgataaat gctcaatctg agctaagacg
      181 actcaattca caactgaatg tattgcaaaa atatgtgaat tctcgtcgct taggttacta
      241 tactcagcag gcagttttag aacaaattag cattattcaa aataaaatta aacaaacaca
      301 aacaatattt aatgacgcta atgcaactgt aaaactcaca gatcaaaagc tagaagaagc
      361 caaattagct ctaggctctg taaacgatct tgtattaata aaagcctctg ctccagcaat
      421 gcaagcaact aatcaagata cgagtatgat gaatattatt caagcagatt ggataagcca
      481 atacgctaac acagcacttt ctgaactctc ggcacaggct aattctgctc tgcaaatcag
      541 taatagctta gatcgccaac tcttcaaaca aagcgataaa ttcaacgtat ggagcagcgt
      601 cgaacatcag aaaaccgagc ataaatcaga tttataccgc ccgtataaac aacaaaccaa
      661 cctgacccaa ctgggcatac aaatgccgat agataacggt ttaatgtttg gagttgcatt
      721 atctaaaaac cacgctaacg cggaatttaa cgagggtgta aacggtaaat cgaatctact
      781 aatggcaagc ctatatggta agtggcaatc tcaacaaggc acttttatca gccttgatgg
      841 cagctacggt aaagcaaaaa accaactcta cctatttggt gaaaaccact ttacccgccg
      901 aatttcctct attggtgcta acattggaca tcaatttgac ctcgcaggag ttcaaattca
      961 gccaacaata ggagcaagat actaccattt cagcggccaa gactatacac taggaggagc
     1021 gaaaatcagc tcaccaaata cccactttat gacatatcaa gcgggtctaa aagctagtaa
     1081 aaccttttca ttggatgact ggaaagttga accaagcatt acaacccact atgtggatgc
     1141 aagtaacaaa cgcttagcag taaacgtcaa taataacaat atcaaccaac gtttcggtcg
     1201 ctatctgaaa accgaagctg gtatccgatt tgaacataac caatggcaat tcgacttaca
     1261 cgctggtttc attaacggca acgaaatcaa aaaacaacat tttgctggtg ttaagctagg
     1321 atatagctgg taagaaaaag cccctagaac tatttctagg ggctttttct actcttaaaa
     1381 agagtatcta acttgcctta ttttctaacc aattcttaat aaaatctgct attcccccaa
     1441 tattattaat atccaaccaa ttttctctct caagcgggta atccgttgcg gtggcaatcg
     1501 tccattggtc tagctctggc aatggttttt caatcccttt gcggtggagc tgaatttttg
     1561 gcaaggtttc gtgtttaaag ccttctacga aaacgagatc aacctcttgc gggttgaatt
     1621 ttgcaattaa ttgagaaaat tcgaccgctt gtttggtttc tgtcattaat gcccaacgtt
     1681 catcgcacac aatcattgtc gggtttgctc ctgcttcacg taggcggtag ctgtctttgc
     1741 cttttttatc tacatcaacg ttatggtggg aatgtttaat cagcccaacc cgaataccgc
     1801 aagcggtcaa ttttgggatt aatttttcca ataaggtggt tttacctgtg ccgctgtagc
     1861 ctgtaattgc aaggtttttt acagggaatt ggcttactcg gcttaaatct tccaatgtat
     1921 tcacgttggt gaatgccgat ttttgttcgg aaaaatcaac tgccacgctt ttttgtgatt
     1981 ggaagaacgc aagcaattgc tgttggtttg agcctaaata ggctttcaaa tcttcttgta
     2041 ccgaacgatg aatcaacgca aaagtgggat gtggacgctc accatcatgg gcatatgcaa
     2101 tttgtacgtt atttatccgc aaagcagttt ttaatttttg caataacagc tcaggcataa
     2161 agggcgtatc gcaaggcaca aaaagcaaat agtcactttc aatttgctca aatcctgcca
     2221 gcattccact gagtggacct tgaaaatcag gcaagctatc ttgatagtaa gaaaaagcag
     2281 gataaagctg ctgatattgc tcaatagaac gattgacatt aagccaaatg tcactaactt
     2341 gtggtgaaag tcgggctaaa atgtgtgaaa taagcggttt tccttcaaaa agttgtaacc
     2401 ctttttctac cccattcatt cgacgagcta aaccgcccgc taaaatcacg gctgaaatgc
     2461 gttcttctat tgttttactc acagctttat cccttaaaat tttgcaaaaa taagagtaaa
     2521 agtatattat ttcgtttaat ttccaacaag ttttttaagg ggtaaaagat gaaatgcgaa
     2581 cgtttagaag agttattaga gttacttggc gaacattggc gtaaaaatcc tgacttacac
     2641 ctcattgata ttttgcagca gctttcagtt gaagtgggcg agcctgataa tttcaaagcg
     2701 t
//
LOCUS       DANLRK010000232         2678 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.233, whole genome shotgun sequence.
ACCESSION   DANLRK010000232 DANLRK010000000
VERSION     DANLRK010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.233"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            139..579
                     /locus_tag="PXA33_002461"
     CDS             139..579
                     /locus_tag="PXA33_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HDL5121752.1"
                     /translation="MTACKNGLGFLVLRLFLAYEFFEAGLSKLNGENWFASIQESFPF
                     PFNVLPADLSWAIASGAEILLPILLTLGLFTRFSALGLMILTAVAWYAVHAGNGYNVC
                     NNGYKMALIYLVMLVPLLLQGAGSLSLDFLLQKKSPTKRWLKFL"
     gene            605..892
                     /locus_tag="PXA33_002462"
     CDS             605..892
                     /locus_tag="PXA33_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121753.1"
                     /translation="MKKATLMALAGALTMATATVNAETKSVKADACMEVKDGKCVKTK
                     ATEGKCGEGKCGATSSEPKATEGKCGEGKCGATAPKAAEGKCGEGKCGASK"
     gene            993..1916
                     /locus_tag="PXA33_002463"
     CDS             993..1916
                     /locus_tag="PXA33_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 family protein"
                     /protein_id="HDL5121754.1"
                     /translation="MLKFTGAGLGYRRDLADGFLSLTDNNIINFMEVAPENWLKMGGQ
                     ARYNFDKVAEKIPVIIHGLSLSLGGQAPLDMTLLKGIKNMQQQYNSFFFSDHLSYCEC
                     EGHLYDLLPMPFTDEAVHHVANRIKQVQDYLGMQISLENTSYYLHSPTSTMNEVEFLN
                     AIAQEADCGIHLDVNNIYVNGVNHGLLDPFVFIDQVDKSRVNYIHIAGHDEDHGAAED
                     VKMTEGESFNMVKGELRHLPTLLVDTHGEAVQKTVWDLLEYTYEHLPHIPPTLLERDF
                     NFPPFQELISEVQHIMSLQKKYAKTRLKNAA"
     gene            1906..2646
                     /locus_tag="PXA33_002464"
     CDS             1906..2646
                     /locus_tag="PXA33_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding domain-containing protein"
                     /protein_id="HDL5121755.1"
                     /translation="MPLKLVEEHKPLLKEIQAEFANAIRHNDVKLMSGNISPQRLGVY
                     SRLVRNNTLGFIDRCYVQLPQHLSPEEWVAVKEKFIREGKAHSPFFQDIAGEFLNFCR
                     ENGCMPPHLLELMDFENAQLLAEVSMATVPSEFEWDNDTIMQFSGAAELRQYAVNFIR
                     SEFKQFDFEPTNVLIWRDTEFGIYYNRLEELDFFLLSSLQEGANSLNGLLAELSEFTE
                     QNLELKALLRQLWEKWVEAEVIYPVYPE"
BASE COUNT          801 a          531 c          571 g          775 t
ORIGIN      
        1 ctataaaaat aggttataat ttttcacaca ttttttgtaa taaacgtata cttattctgt
       61 ctaataatca catttttagt taggagcatt tttatgtcat cattttcgtc atcattttca
      121 actcagtcag cttgctcaat gacagcttgt aaaaacggct tgggctttct tgtattacgt
      181 ctatttcttg cttatgaatt ttttgaagca gggttatcaa aactaaacgg tgaaaattgg
      241 tttgcctcaa ttcaagagag cttcccattc ccctttaacg tactaccagc cgatcttagc
      301 tgggctattg ctagcggagc ggaaatctta ctgcctattt tactcacttt gggtctgttc
      361 acccgcttca gtgcgttagg attaatgatc ttaacagcgg tagcgtggta tgctgttcac
      421 gctggcaatg gctacaatgt ctgcaacaat ggctataaaa tggcactcat ttatttagtg
      481 atgttagtac cacttttatt acaaggtgct ggctcgctct ctctcgattt tctgttacag
      541 aaaaaatccc caaccaaacg ctggttgaaa ttcctttaat cccttaattt atggagaatt
      601 tttaatgaaa aaagcaactt taatggcatt agctggtgca ttaactatgg caaccgcgac
      661 tgtaaacgct gaaactaaat cagtgaaagc agatgcttgt atggaagtaa aagatggcaa
      721 atgtgtaaaa accaaagcta cagaaggtaa atgtggcgaa ggcaaatgtg gagcaacctc
      781 ttctgagcca aaagcaaccg aaggtaaatg cggcgaaggg aaatgtggag caacggcacc
      841 aaaagcggca gaaggtaaat gcggtgaagg caagtgtgga gcttctaagt aagtagcgaa
      901 ggtgcgtttt ctaaacgcac ctaacctctt ataaacacaa tctacaagcg gttaaattcc
      961 gctgattttt tgcaaattaa aggaacaaga cgatgttaaa atttactggt gccggtctag
     1021 gctatcgccg tgacctagcg gacggatttt tatctctcac agataacaat ataattaatt
     1081 ttatggaagt agccccagaa aactggctga aaatgggcgg tcaggctcgg tataacttcg
     1141 ataaagtcgc tgaaaaaatc cctgttatta ttcacggttt atccctctct ctcggcgggc
     1201 aagcgccttt agatatgacg ctactaaaag ggatcaagaa tatgcagcag cagtataact
     1261 cctttttctt ctctgatcat ttaagctatt gcgaatgtga agggcattta tatgacctgc
     1321 tgccaatgcc gtttaccgat gaagccgtac atcacgtggc aaatcgcatt aaacaagtgc
     1381 aagattattt aggtatgcaa atttcccttg aaaatacgtc ctactatctg cattcaccaa
     1441 ccagtacgat gaatgaggta gagtttctca atgcaattgc ccaagaagcg gattgtggca
     1501 ttcaccttga tgtgaataat atttatgtta atggtgtcaa tcacggcttg cttgatccat
     1561 ttgtatttat tgatcaagtg gataaatctc gtgtcaatta tattcatatt gcaggccacg
     1621 atgaagatca cggtgcggcg gaagacgtta aaatgaccga gggtgaatca tttaatatgg
     1681 taaaaggcga actacgtcat ctgccaactt tactggtcga tacacacgga gaagctgtac
     1741 aaaaaactgt gtgggattta ctagagtaca cctatgaaca cctaccgcat attccaccaa
     1801 cactgttaga acgggatttc aacttcccac cgtttcaaga actgattagt gaagtacagc
     1861 acattatgag cttacagaaa aaatatgcaa aaacgaggct aaaaaatgcc gcttaaactc
     1921 gttgaagaac acaaaccgtt actcaaagag attcaagccg agtttgcgaa tgcaattcgc
     1981 cataacgatg taaagctgat gagtggtaat atctctccac agcggcttgg cgtctattct
     2041 cgcttggttc gcaataatac tctcggtttt atcgaccgtt gttatgtaca gctccctcaa
     2101 catctaagtc ctgaagaatg ggttgccgtt aaagaaaaat ttatacgaga aggtaaggct
     2161 cattcgccct ttttccaaga tattgctggc gaatttctca atttttgccg agaaaatggc
     2221 tgtatgccac cacacttgct tgagttgatg gactttgaaa atgctcaatt actggctgag
     2281 gtatcaatgg caaccgtacc atcagaattt gaatgggata acgatactat aatgcagttt
     2341 tctggtgcag ctgagctgcg tcaatatgcg gtaaacttta tccgtagcga gttcaaacag
     2401 tttgactttg agccgaccaa cgtactgatt tggcgagata cggaatttgg catctattac
     2461 aaccgcttgg aagagctgga tttcttcctg cttagctcgt tgcaagaggg ggcaaattcc
     2521 ctgaatggct tactagcaga actcagcgag tttaccgagc aaaatctgga gctaaaagcc
     2581 ttgttacgcc aactttggga aaaatgggtt gaagcagagg tgatttatcc tgtctatcct
     2641 gaatgaggat aaggtatgtg atatgtactg acatacat
//
LOCUS       DANLRK010000233         2608 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.234, whole genome shotgun sequence.
ACCESSION   DANLRK010000233 DANLRK010000000
VERSION     DANLRK010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.234"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            81..1196
                     /gene="wecB"
                     /locus_tag="PXA33_002465"
     CDS             81..1196
                     /gene="wecB"
                     /locus_tag="PXA33_002465"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015703970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HDL5121756.1"
                     /translation="MRYLIVFGTRPEAIKMAPLVEGFKKSGLNFKVCVTAQHRQMLDQ
                     VLELFEITPDYDLDIMSNKQTLSTVTSAILEKIQPVIAEYKPTTIFVHGDTATTLASS
                     LAAYYNQIDIAHIEAGLRTHNIYSPWPEEGNRKLTAALAKYHFAPTQSTKKNLLNENI
                     AEGTIYVTGNTVIDALFLACKKLDENIDLLSSLQEKFSYIKSKRTILITGHRRENFGN
                     GFENICKAVSTLASDFPDVQFIYPVHLNPNVREPVNRLLADKSNVHLIEPCDYLSFVY
                     LMRESYLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVEAGTVKLVGTAQSEIIKQV
                     TELLNDQAAYTKMSEAHNPYGDGTAVKQILSVFQSKQ"
     gene            1211..2482
                     /gene="wecC"
                     /locus_tag="PXA33_002466"
     CDS             1211..2482
                     /gene="wecC"
                     /locus_tag="PXA33_002466"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HDL5121757.1"
                     /translation="MSNFNTISVVGLGYIGLPTATVFAQHGLNVIGVDVNQHAVDHIN
                     QGKIHIVEPDLDVAVHQCVSSGKLKATLTPEPAEAFLIAVPTPFKGEDYEPDLSYIQA
                     ACKAIAPVLEKGNLVILESTSPVGATEQMAEWLAEYRPELSFPQQAGEDSDIRIAHCP
                     ERVLPGQVMHELIENDRIVGGMTNKCSVQAVNLYKTFVKGDCIVTNARTAEMCKLTEN
                     SFRDVNIAFANELSIICDRLDINVWELIALANRHPRVNILQPGCGVGGHCIAVDPWFI
                     VNKTPDLAKIIRTAREVNDHKPEWVISKMNEAVIYALQKTGKSIDQIKIACLGLAFKP
                     DIDDLRESPALKITEKLAEKYPNQVFAVEPNVEELPKKLANKNISLISVDEALEVADV
                     VVVLVDHSEFKLVKPVFLNDICVVDTKGIWR"
BASE COUNT          848 a          496 c          500 g          764 t
ORIGIN      
        1 acgatcttta taactaattt aacaaaatta cttacatcta gtgtatttta gttttatcta
       61 tatttttaaa ggagtaataa atgcgctatc tcatcgtctt cggtactcgt cctgaggcaa
      121 tcaaaatggc tcccttagtt gagggcttta agaaaagcgg tctaaatttt aaagtctgtg
      181 ttactgctca gcaccgccaa atgctggacc aagttttaga attattcgaa attactccag
      241 attatgacct ggatattatg agcaataagc aaacactttc tactgtaact tctgctattt
      301 tagaaaaaat tcaacctgtt attgccgaat acaaaccaac tactattttt gttcatggcg
      361 atacagcaac cactcttgcc tcttcattag cagcctatta caaccaaatt gatatcgctc
      421 atattgaggc aggtctaaga acgcataata tctactctcc ttggccggaa gaaggcaatc
      481 gcaaattaac agctgctttg gcaaaatacc atttcgcacc aacacaaagc accaagaaaa
      541 atctactcaa cgaaaatatt gctgagggca ccatttatgt aacgggtaat acggtaattg
      601 atgccttatt cctcgcttgc aaaaaattag atgaaaatat tgatttatta agctcgctac
      661 aagagaaatt ttcttatata aaaagtaagc gtactatttt gatcaccgga catcgccgcg
      721 aaaatttcgg taatggtttt gagaacatct gtaaagccgt ttcaacatta gcgtctgatt
      781 tccctgacgt tcaatttatc taccccgtac acctcaaccc aaatgtaaga gaaccggtca
      841 atcgcctatt agcggataaa tccaatgtgc atctcattga gccttgcgac tatctctcct
      901 ttgtttattt aatgagagag tcttacctta tcttaactga ctccggaggt attcaagaag
      961 aagccccatc attaggaaaa ccggtattag taatgagaga cacaacagaa agaccggaag
     1021 ctgtagaagc cggaacagta aaacttgttg gaactgcaca atctgaaatt attaaacaag
     1081 ttactgagtt actcaatgat caagctgctt atacaaaaat gtctgaggct cacaaccctt
     1141 atggcgatgg cacagctgtg aaacaaattc tctcagtatt tcaatcaaaa caataataag
     1201 ggataaatgt atgtcgaact ttaataccat ttcagtagta ggcttaggct atattggctt
     1261 accaaccgcc actgtatttg cccaacacgg tctaaatgta attggcgtag atgttaatca
     1321 gcacgccgta gaccatatca atcaagggaa aattcacatt gtcgagcctg atttagatgt
     1381 tgctgttcac caatgtgtat ctagcggaaa attaaaagcc acactgacac ctgaaccagc
     1441 agaagcattt ttaattgctg taccaacgcc ttttaaaggc gaggattatg agcctgattt
     1501 aagttatatt caagctgctt gcaaagcaat tgcacctgtg ctggaaaaag gtaatttagt
     1561 cattttagaa tcaacctctc cggttggtgc aacagaacag atggctgagt ggcttgccga
     1621 gtaccgtcca gagctaagct tccctcagca agcaggtgaa gattctgata ttcgtatcgc
     1681 ccattgccca gaacgagttc taccaggaca agtgatgcat gaattgattg aaaacgaccg
     1741 tattgtgggt ggtatgacaa ataaatgctc tgtgcaagcg gtcaatttat acaaaacttt
     1801 tgtaaaaggc gactgtatag ttaccaatgc ccgtaccgca gaaatgtgta aattaaccga
     1861 aaatagtttc cgtgatgtca atatcgcttt tgcaaatgag ctttctatta tttgtgatcg
     1921 cctagatatt aatgtatggg aactaattgc tttggctaac agacaccctc gagtcaatat
     1981 cttacaaccg ggttgtggtg taggtggaca ctgcattgct gtagatcctt ggtttattgt
     2041 gaataaaact cctgacttgg caaaaattat ccgcactgct cgtgaggtaa acgatcataa
     2101 acctgaatgg gttatctcaa agatgaatga agcggtaatt tatgccttac aaaaaacagg
     2161 taaatctatc gatcaaatca aaattgcttg tttagggttg gcattcaagc ctgatattga
     2221 cgatttgcgt gaaagtccgg cattaaaaat tactgaaaaa ttggcggaaa agtatccaaa
     2281 tcaagtattt gcagttgagc cgaatgttga agagctaccg aaaaaattgg ctaacaagaa
     2341 catttctttg atttctgttg atgaagctct ggaagtagct gatgttgtag ttgttttggt
     2401 ggatcatagc gaatttaaac tagtcaaacc tgtgtttttg aatgatattt gtgtggtgga
     2461 tactaaagga atttggagat aagtgatgaa aaagaaacag caatatcgta aatccactat
     2521 taacaatact aatgcatcac aacaaaattc aggcgaagta ttaaattctg tcaattatca
     2581 aacattactg gaagattatt taaataaa
//
LOCUS       DANLRK010000234         2596 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.235, whole genome shotgun sequence.
ACCESSION   DANLRK010000234 DANLRK010000000
VERSION     DANLRK010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.235"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(195..2471)
                     /gene="gshAB"
                     /locus_tag="PXA33_002467"
     CDS             complement(195..2471)
                     /gene="gshAB"
                     /locus_tag="PXA33_002467"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HDL5121758.1"
                     /translation="MKLQKLIRENHLALLFQQGNFGLEKESQRVDRNGNIVTTPHPAV
                     FGNRSYHPYIQTDFAESQLELITPPNAKLEDSFRWLSAIHEVVLRSLPEEEYIFPLSM
                     PAGLPPEEQIQEAQFDNPEDVQYRQHLSKNYGKYKQMVSGVHYNFQLSTEFVEKAFAL
                     QTEYSELKAFQNALYMKLANNFLRYQWILLYLLAATPTVESQYFYEKSPLARGQLVRS
                     LRSSPYGYVNSEKVVVNHDSLESYVESLEANVANGLLIAEKEFYSNVRLRGGKKAREL
                     LENGVKYAEFRLFDLNPFAPYGIELNDAKFIHYFLLGMLWLEETSGQKEVEIGKQKLY
                     QVALEDPRTETAFRAEGEAILNQLLAMLEELSAGQESLDLVREKLAQFADPSKTLNGR
                     LIAAIEEVGSYKALGAKLAQTYKAQAFERFYALSAFDNMELSTQALFFDLIQQGIEVE
                     VLDENDQFLALKFGDHLEYVKNGNMTSRDQYISPLIMENKVVTKKVLAKAGFNVPKSV
                     EFTNLEQAVAHYPLFEGKAVVIKPKSTNYGLGITIFQQAVKNREDFAKAVEIAFREDK
                     EVMVEDYLVGTEYRFFVLGDETLAVLLRVPANVVGDGIHSVKELVERKNDDPLRGDGS
                     RSPLKKIALGEIEQLQLKEQGLTVDSVPAKGQTVQLRANSNISTGGDSIDMTDQMHES
                     YRQIAVGIAQAMGAKVCGVDLIIPDLTKPAEPNLSSWGVIEANFNPMMMMHIFPYQGK
                     SRRLTKAVIKMLFPEIGN"
BASE COUNT          710 a          558 c          526 g          802 t
ORIGIN      
        1 ataaattggg ctaaatgtct tttctttata acattttgtt aacggagata ttttcgatcg
       61 tttttactta tacaaaaaaa ttcttctttt ggagtgtttt gcacaaagtg aaatttaatt
      121 ggagtaaaac tttagaaata aattaaggag aattcttgag taaattctcc ttaaaagagg
      181 tatagagaga aaatttaatt gcctatctca ggaaacagca ttttgattac cgctttggtt
      241 aaacggcggg atttgccttg gtaagggaaa atgtgcatca tcatcattgg gttgaagttt
      301 gcctcaatga cgccccaaga gcttaaattt ggctcggcag gtttggtgag atcaggaatg
      361 atcaaatcca ccccacacac tttagccccc attgcctgag caattcccac tgcaatttgt
      421 ctatagcttt cgtgcatttg gtcggtcata tcaatcgaat cgccgccggt gctgatgttg
      481 gaattggcac gcagttgaac tgtttgacct ttcgccggta cggaatctac ggttaagcct
      541 tgctctttaa gttgtaactg ctcaatttca cctaaggcaa tttttttcaa cgggctacga
      601 gagccatcgc cacgtagcgg atcgtcattt ttacgctcca ccagctcttt tacactgtga
      661 atgccatctc ccactacatt cgccggtaca cgcagtaaca ccgcaagggt ttcatcgccc
      721 agtacaaaga agcggtattc agtaccgact aaatagtctt ccaccatcac ctctttgtct
      781 tcacggaacg caatttcaac cgcttttgca aaatcttctc gatttttgac cgcttgttgg
      841 aagatggtaa tccctaaacc atagttagtc gatttcggct taatcaccac cgctttgcct
      901 tcaaatagcg gataatgtgc cacagcctgt tcaaggttgg taaattcaac gcttttcggc
      961 acgttaaagc cggcttttgc caatactttt ttcgtcacca ctttgttttc cataatcagc
     1021 ggtgaaatgt attgatctcg gctggtcata ttgccgtttt tcacatattc caaatgatcg
     1081 ccaaatttta gggcgaggaa ttgatcgttt tcgtccagta cttccacctc aatcccttgt
     1141 tggatcaaat caaaaaacag ggcttgagtg gaaagttcca tattatcaaa tgccgataac
     1201 gcataaaaac gctcaaaggc ttgtgcctta taggtttgag ccaatttagc ccccaatgct
     1261 ttataactgc cgacttcctc tattgcggca attaaacgtc cgttcagggt tttgcttgga
     1321 tcagcaaatt gagctaattt ttcccgcacc aaatctaggc tttcctgacc ggcagataat
     1381 tcttcaagca tcgctaaaag ttgatttaaa atggcttcac cttcggcacg gaaagcggtt
     1441 tctgttcttg gatcttcaag tgcgacttgg taaagtttct gtttgccgat ttcgacctct
     1501 ttttgaccgc ttgtttcctc cagccaaagc ataccgagta agaaataatg gataaatttg
     1561 gcatcattca gctcaatgcc gtaaggggcg aaagggttta aatcaaataa acggaattcg
     1621 gcgtatttca caccgttttc caatagctca cgggcttttt tgccccctcg taaacgcaca
     1681 ttggaataga attctttttc ggcaattaat aatccgttgg caacattggc ttcaaggctt
     1741 tcaacatagc tttctaagct gtcgtgatta accaccactt tttccgaatt cacataacca
     1801 taagggcttg aacgcaagct gcggacaagt tgccctctcg caagcggtga tttttcataa
     1861 aaatattgcg attccaccgt aggcgtggca gccaataaat agagcaaaat ccattgataa
     1921 cgcaaaaagt tattagctaa cttcatataa agtgcgtttt ggaaggcttt cagctcgcta
     1981 tattccgttt gtaaagcaaa ggctttttcg acaaattccg ttgaaagttg aaaattataa
     2041 tgcacgccgc ttaccatttg cttatatttc ccatagtttt tggaaaggtg ttggcgatat
     2101 tgcacatctt cagggttgtc aaattgtgct tcttgaattt gctcttccgg cggcaagcct
     2161 gccggcatac tgagagggaa gatatattcc tcctctggca acgaacgcaa taccacttcg
     2221 tgaatagcag agagccaacg aaaactgtct tctaatttag cattcggggg agtgattaac
     2281 tctagctgac tttcggcaaa atcggtttga atataagggt ggtaagagcg attaccgaat
     2341 acggcagggt ggggagtagt aacgatattg ccgttgcgat caacccgttg gctctctttc
     2401 tcaagcccaa aattcccttg ttgaaacagc aatgctaaat gattttctcg gataagtttt
     2461 tgtagtttca tcatctatcc tttgtagtca aaaaaatgta tcggcacaat aacacaaatg
     2521 aaagacttag tgaaatagtg ggttttagtt tattggaatg aagtagatga tttggttata
     2581 acagaaatac tgcttc
//
LOCUS       DANLRK010000235         2592 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.236, whole genome shotgun sequence.
ACCESSION   DANLRK010000235 DANLRK010000000
VERSION     DANLRK010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.236"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            1..732
                     /locus_tag="PXA33_002468"
     CDS             1..732
                     /locus_tag="PXA33_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011978780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL5121759.1"
                     /translation="MLRLINNNGVASINFLAEQLKVSHMTIRRDIQKLEEQGKVSLVS
                     GGVQSLERLIMELPHDDKSMLFQEQKSAIGSLASQLIPSNTTIYLDAGTTTLEIAHRI
                     IDRNDLLVVTNDFVIAHYLSNSGKCDLIHIGGSVCKENLSTTGSLAAEFLKNLSIDMA
                     FISTSSWNLKGLTTPNENKIPVKKAILEASKQNILVTDSSKYGKVATFFVCPLTVFDQ
                     IICDKGLFENVRDALKGLSIKLDLG"
     gene            complement(872..2263)
                     /gene="ffh"
                     /locus_tag="PXA33_002469"
     CDS             complement(872..2263)
                     /gene="ffh"
                     /locus_tag="PXA33_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011199698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HDL5121760.1"
                     /translation="MFENLSDRLSKTLRNITGKGRLTEDNIKDTLREVRMALLEADVA
                     LPVVRDFINKVKERALGVEVNKSLTPGQEFLKIVQSELETAMGEANEELNLAAQPPAV
                     ILMAGLQGAGKTTSVGKLAKFLKERHKKKVLVVSADVYRPAAIKQLQTLAEALKVDFF
                     PTETSQKPVAIAEAALKHAKLNFFDVLIVDTAGRLHVDGEMMEEIQQIHAVLNPIETL
                     FTVDAMTGQDAANTAKAFNEALPLTGVILTKVDGDARGGAALSIRQITGKPIKFLGVG
                     EKTDALEPFHPDRVASRILGMGDVMSLIEDIQRSVDQEKAEKIAAKFKKGDEFTLEDF
                     REQLIEMKKMGGMMSMLDKLPGAKNLPDHVKSQVDDKMFMKMEAIINSMTLKERANPD
                     IIKGSRRRRIALGSGTQVQDVNKLLKQFDDMQRMMKKMRKGGMAKMMRGVKGMMGGGL
                     GGLGGLGSMFGKR"
BASE COUNT          773 a          546 c          505 g          768 t
ORIGIN      
        1 atactacgac ttattaataa taatggtgtc gcaagtatta attttctggc agaacagcta
       61 aaagtatcac atatgacaat tcgccgtgat attcaaaagc tggaagagca aggtaaggta
      121 tccctagtat ctggtggagt tcaatctctt gaacgattaa tcatggaatt accacacgat
      181 gataaatcaa tgttattcca agagcagaaa tcagctatag gatctttagc atcacagcta
      241 attccctcaa acacaactat ttatcttgat gctggaacta ctacattaga aatagctcat
      301 cggataatcg atagaaatga cttactagtt gtaacaaacg attttgttat tgctcactat
      361 cttagtaatt caggaaaatg tgatttaatt catataggag gaagtgtctg taaagaaaac
      421 ctatcgacaa ctggttcact tgcagcagaa tttctgaaaa acctatcaat cgatatggca
      481 tttatatcta cttcatcctg gaatttaaaa ggtctaacaa cgccgaacga aaataaaatc
      541 cccgttaaaa aagcaatttt agaggcatcc aagcaaaaca ttttagtgac agactcttca
      601 aaatatggaa aagtcgcaac tttttttgtt tgccctctta ctgtatttga ccagattatt
      661 tgtgataaag gattatttga gaatgtacga gacgcactga aagggctaag tattaaatta
      721 gatttaggat aatcttctta ccaatttttt ctgagaacga caagcaatca aaaagtatta
      781 tttttgcaaa taagatatta atagggaaag ttaacaatcc tagcaacgga ttgttaactg
      841 attttcggct ggtactaata aaataggaat attaacgttt accaaacata ctgcctaagc
      901 caccgagacc gcctagacca ccacccatca tacctttaac accacgcatc atttttgcca
      961 ttccgccttt acgcattttt ttcatcatac gttgcatatc atcaaactgc ttgagcagct
     1021 tatttacatc ttgcacctgt gtgccggagc caagtgcgat acggcggcgg cgtgagcctt
     1081 taataatatc cggatttgcc cgctctttta aggtcataga attgatgatg gcttccattt
     1141 tcatgaacat tttatcatca acctgacttt taacgtggtc aggcaggttt ttcgcccccg
     1201 gtagtttatc gagcatcgac atcattccac ccattttttt catttcgata agctgctcac
     1261 ggaaatcttc caaggtaaat tcatcgcctt ttttgaattt ggcggcaatt ttttccgctt
     1321 tttcttgatc aaccgagcgt tgaatatctt caattaaaga cattacatcg cccataccta
     1381 aaatgcggga agccacacgg tcagggtgga acggctcaag ggcatcagtt ttttcaccca
     1441 cacctaagaa tttaatcggt ttaccggtga tttggcgaat agaaagtgcc gcaccgcctc
     1501 gggcatcgcc atcgactttg gtaagaataa caccggtaag cggcaacgct tcgttaaagg
     1561 cttttgcggt gttggcggcg tcttgaccgg tcatcgcatc aaccgtaaat agcgtttcaa
     1621 tcggattcag cacagcgtgg atttgttgga tctcttccat catttcgcca tcaacgtgta
     1681 aacgaccggc agtatccaca atcaatacat caaagaaatt aagcttagcg tgcttcagtg
     1741 ccgcttctgc aatcgctacc ggtttttggc tggtttcggt cgggaagaag tccactttta
     1801 acgcttcagc aagggtctgt agctgcttga tagcggcagg gcggtaaaca tcggcagaaa
     1861 ctaccaacac ctttttcttg tggcgttctt tcaggaattt tgccaatttg ccgacagagg
     1921 tggttttacc cgcaccttgc aaacctgcca ttaaaattac cgcaggcggt tgggccgcaa
     1981 gattcagctc ctcgttggct tctcccattg ccgtttctaa ttcgctttgt acgattttta
     2041 agaactcttg ccccggcgtg aggcttttgt ttacctcaac ccctaacgca cgctctttga
     2101 ccttgttgat gaaatcacgc acaacaggga gggcaacatc cgcctccaac aatgccatac
     2161 gaacttcacg cagggtatct ttaatattat cttccgttag gcgacctttg cccgtaatgt
     2221 tacgcagcgt tttagacagt ctgtcagaga ggttttcaaa catatcagct ttctcaattt
     2281 gcaaaatttt aggaaaaaag taccgcttgt gagcggaaag ttgccgccat tatacaaaaa
     2341 aaagcgttac ggttataggg aaataaaaaa acggcatagc gattaaacta tgccgtaaaa
     2401 tttgacgata ttcgaggaat cattatttag ttatattgtg cgatcataat actccgaatt
     2461 gcgtataaaa gtgaagtgat attttcaaat ttatgttaga aatgagaggt aaattacaaa
     2521 attaaagggg atattattgt ttgaaaaaat aagagcgaaa agaaaaccct gatgattgct
     2581 catcagggct tc
//
LOCUS       DANLRK010000236         2586 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.237, whole genome shotgun sequence.
ACCESSION   DANLRK010000236 DANLRK010000000
VERSION     DANLRK010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.237"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            <1..617
                     /locus_tag="PXA33_002470"
     CDS             <1..617
                     /locus_tag="PXA33_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121761.1"
                     /translation="NLAAISANLGKVTAGTITGTTISGGTISGTTITGGIIKGARIEG
                     LTGKFTGELEINQLIGGNIYEAKTWTSKATGKSKTYSETKNSFALSDTYYEYVCQFVL
                     PATKATRTLYVVHTPTGESKSETFYGSGSNRTPTTITLPSVIDISNTISPNVSNPFND
                     SGAILIANTQYVVTCRAWCAGRGRSAKVNFRAIISSTNGSSYQL"
     gene            627..785
                     /locus_tag="PXA33_002471"
     CDS             627..785
                     /locus_tag="PXA33_002471"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121762.1"
                     /translation="MKKIILLTTIMVSACTTQYKQDYTTKPTFCYQLAPQEQLPGKNC
                     IGSGGHSG"
     gene            complement(845..1885)
                     /locus_tag="PXA33_002472"
     CDS             complement(845..1885)
                     /locus_tag="PXA33_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HDL5121763.1"
                     /translation="MFYSNNPLIKHKTGLLNLAEELGNISQACKVMGMSRDTSYRYQQ
                     AVEQSGVDALLNQNRRVPNLKNRVDEATEQAVVKFAFDNPAFGQVRVSNELRKQGIFI
                     SAGGVRSIWLRHNLANFKQRLNALEKAVAEKGIILSENQVQALERKKEDDVACGEIET
                     AHPGYLGSQDTFYVGNLKGVGRIYQQTFIDTYSKVAFAKLYTMKTAISAADMLNDKVL
                     PFFESQGLPMLRILTDRGSEYCGKVENHDYELYLAINDIEHTKTKVKHPQTNGICERF
                     HKTILQEFYQVAFRKKIYTDLTTLQADLDEWLMYYNHHRTHQGKMCCGRTPMATLLDG
                     KRIWVEKNLSSN"
     gene            complement(2003..2302)
                     /locus_tag="PXA33_002473"
     CDS             complement(2003..2302)
                     /locus_tag="PXA33_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDL5121764.1"
                     /translation="MKTLDQMLNELSPERRQKVEDMAQEMIREIQLSKLREELNLSQQ
                     QLATALGISQPAIAKLEQKDNDPRLSTLKRYIEAMGGKLKLAVELPTGDERVIRI"
     gene            complement(2274..>2586)
                     /locus_tag="PXA33_002474"
     CDS             complement(2274..>2586)
                     /locus_tag="PXA33_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL5121765.1"
                     /translation="QERVLACLIYLRKFGYSLGRPYADTVYQSKFPNMKELRIQNKGK
                     PIRAFYAFDPLRQAIVLCAGEKGKDKRFYEKMIALADAEFTSYLNNLEKENENVRSNA
                     Q"
BASE COUNT          774 a          515 c          483 g          814 t
ORIGIN      
        1 acaatttagc ggcaatatcg gcaaatcttg gtaaggtaac tgctggtaca attacaggga
       61 cgacaattag tggtgggaca atttcaggta caacaattac agggggaata attaaaggag
      121 caagaattga gggattaaca ggtaaattta ctggagaact tgaaatcaac caattgatcg
      181 gaggtaatat ctatgaggct aaaacttgga catcaaaagc aacagggaaa agtaaaacat
      241 attcagaaac gaaaaacagt tttgcattaa gtgatacata ttatgagtat gtatgtcaat
      301 ttgtactacc tgctacaaaa gcgacgagaa cattgtatgt tgtccacact ccaacaggag
      361 agagtaaaag cgaaactttt tatggtagtg gtagtaatcg gacaccgaca acaattacat
      421 taccgtctgt aattgatatt agcaatacta tttcacctaa tgtatctaac ccatttaatg
      481 attctggggc aatactcatt gctaatactc aatatgtagt aacgtgtcgt gcgtggtgtg
      541 cagggcgtgg gagaagtgct aaagtgaatt ttcgagcaat tatatcatct acaaatggtt
      601 catcttatca attataggag gcaaatatga aaaaaattat actacttaca acaattatgg
      661 tttcagcttg tacaactcaa tataagcaag actatacaac aaaaccaacc ttttgttatc
      721 aactagcccc tcaagagcaa ttaccgggga aaaactgtat tggctcagga gggcattcag
      781 gttaactgta gaagatcaaa cctaatctga cagtcccccg ttttaaatta ccgtgtctgt
      841 cagattaatt tgagcttaaa ttcttttcca cccaaatccg ttttccatca agtaaggttg
      901 ccatcggtgt tctgccacag cacatttttc cttgatgtgt tcgatggtga ttataataca
      961 ttaaccactc atctaaatcc gcttgtaatg tcgttaaatc tgtataaatt ttcttcctaa
     1021 atgccacttg gtaaaattct tgtaagattg ttttatggaa ccgttcgcag ataccattcg
     1081 tctggggatg cttcactttc gttttcgtat gctctatgtc atttatcgct aaataaagct
     1141 cataatcgtg attttccacc ttgccacagt actcactgcc tcggtcggta agaatacgca
     1201 acatcggtaa tccttgggac tcaaagaacg gtaggacttt atcattgagc atatctgctg
     1261 cactaattgc tgtcttcatc gtatacagtt ttgcaaatgc gactttacta taagtatcaa
     1321 taaaggtctg ttgataaatg cgtccaaccc ccttaagatt ccccacatag aaggtatctt
     1381 gtgaacctag atagcccgga tgagccgttt caatttcgcc acaagccaca tcgtcttctt
     1441 ttttacgctc caaggcttga acttggtttt cactcagaat aatgcctttc tcagccactg
     1501 ctttttctaa cgcatttaaa cgctgtttaa agttagcgag attatggcgt agccaaatag
     1561 aacgcacacc acctgctgaa ataaaaatac cttgtttacg gagttcgtta ctgactcgta
     1621 cctgcccaaa tgccggatta tcgaatgcaa acttcacaac agcttgctct gttgcctcat
     1681 ccacacgatt ttttagatta ggaacacgcc gattttgatt aagtaaggca tccaccccac
     1741 tttgctcaac tgcttgttga tagcgatagg atgtatctcg actcatcccc atcaccttac
     1801 aggcttgaga gatattgccc agttcttctg ctaaatttaa taaaccggtc ttgtgtttaa
     1861 tgagagggtt gttagaataa aacatgagag tttccttttt tgtttagatt gaatttggac
     1921 actcatattc taaacgggaa actctcactt tttagagtga attgtcagat caagtctgat
     1981 cttctacagg ttaacaaagt aattaaatcc taattacacg ctcatcgcct gtaggcaatt
     2041 caacggcaag ttttaactta ccgcccattg cttcaatata gcgtttgagc gtggataatc
     2101 ttggatcgtt atctttctgt tcaagtttag caatagcagg ctgtgaaata ccgagagctg
     2161 tagctaattg ttgttgagag agatttagct cttctcttag ttttgaaagt tgtatctctc
     2221 taatcatttc ttgagccata tcttccactt tttggcggcg ttcaggagag agttcattga
     2281 gcatttgatc taacgttttc attttctttc tctaaattat ttaaatagga ggtaaattcc
     2341 gcatcagcaa gagcgatcat tttttcatag aatcttttgt ctttaccttt ctcgccagca
     2401 caaagtacta tcgcttggcg taaagggtca aaagcataaa atgctcgaat tggcttacct
     2461 ttgttttgaa tgcgtaactc tttcatatta gggaatttag actgataaac tgtatcagca
     2521 taaggtctgc ctagactata accgaatttc cgcagataga ttaaacaagc caagacacgt
     2581 tcttga
//
LOCUS       DANLRK010000237         2558 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.238, whole genome shotgun sequence.
ACCESSION   DANLRK010000237 DANLRK010000000
VERSION     DANLRK010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.238"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            118..408
                     /locus_tag="PXA33_002475"
     CDS             118..408
                     /locus_tag="PXA33_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HDL5121766.1"
                     /translation="MALRPLHDKVILKREEVETKSAGGIVLTGSAATKSTRGKVIAVG
                     TGRILDNGSVQPLAVKVGDVVIFNEGYGVKTEKIDGEEVLILAEHDILAIVE"
     gene            477..2117
                     /gene="groL"
                     /locus_tag="PXA33_002476"
     CDS             477..2117
                     /gene="groL"
                     /locus_tag="PXA33_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011608625.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HDL5121767.1"
                     /translation="MAAKDVKFGNDARVKMLKGVNILADAVKVTLGPKGRNVVLDRAY
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIVNE
                     GLKAVAAGMNPMDLKRGIDKAVAAVVEELKAISKPCETSKEIEQVGTISANSDSTVGQ
                     LIAQAMEKVGKEGVITVEDGTGLEDALDVVEGMQFDRGYLSPYFINKPEAGTVELENP
                     YILLVDKKVSNIRELLPVLEGVAKAGKPLLIIAEDIEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIAILTAGTVISEEIGMELEKATLEELGQAKRVVISKDNTTIID
                     GIGDETQIKGRVAQIRQQIEDSTSDYDKEKLQERVAKLAGGVAVIKVGAATEVAMKEK
                     KDRVDDALHATRAAVEEGIVPGGGVALVRAANKVADTLKGDNEEQNVGIKLALRAMEA
                     PLRQIVANAGEEASVVARNVKEGSGNYGYNAGTEQYGDMLEMGILDPTKVTRSALQFA
                     ASIAGLMITTECMITDLPKEEKLDPAAMGGMGGMGGMM"
     gene            complement(2242..2556)
                     /locus_tag="PXA33_002477"
     CDS             complement(2242..2556)
                     /locus_tag="PXA33_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5339 domain-containing protein"
                     /protein_id="HDL5121768.1"
                     /translation="MLGITLCTILVSSNAIASVKTSSLTVAKPTLSKNLPTECEKMFN
                     TANKLVSDAERQPGTHTQVLKMKNKLSLTKQQILKMDSELQQKSCEKGLTALNSLKQK
                     HQ"
BASE COUNT          809 a          419 c          615 g          715 t
ORIGIN      
        1 aaattttaca aatttgaccg cttgcacttg aaactctaaa atctgccttt atattgttag
       61 gcgttacgca ttttgcgtga agttttattt attcattttt aagaaaagga acatattatg
      121 gcacttcgtc cattacacga taaagttatt ttaaaacgtg aagaagttga aacaaaatcg
      181 gcaggcggta ttgttttaac cggttcggca gcaacaaaat caacccgtgg caaagtaatt
      241 gcagtaggaa caggtcgcat tttagacaat ggttctgttc agcctttagc agttaaagtg
      301 ggcgatgtag tgatttttaa cgaaggctac ggcgtaaaaa ccgagaaaat tgacggcgaa
      361 gaagtgctaa ttttagcaga gcacgatatt ttggcgattg tagaataatt tcgttctgca
      421 caagcggtta aaaaagcaaa attttttaca tatttcaaat acaaaggatt tagaaaatgg
      481 cagcaaaaga cgtgaaattt ggtaacgatg cacgcgtaaa aatgttaaaa ggcgtgaata
      541 ttttagccga tgcggtaaaa gtaactttag gtccgaaagg tcgtaatgtg gtgttagatc
      601 gtgcttatgg tgcaccaacg attacaaaag acggtgtgtc ggttgcccgt gaaatcgaat
      661 tagaagataa atttgaaaat atgggggctc aaatggtgaa agaggttgcc tctaaagcca
      721 atgatgccgc cggtgacggt acaactaccg caactgtatt agctcaagcg attgtaaacg
      781 agggcttaaa agcagtggct gcgggtatga acccaatgga tttaaaacgt ggtatcgata
      841 aagcggttgc agcagtggtt gaagagttaa aagcgatttc aaaaccgtgc gaaacctcaa
      901 aagaaattga gcaagtaggg acgatttctg caaactcaga ttcaacagtc ggtcaattaa
      961 ttgctcaagc aatggaaaaa gtgggcaaag agggcgtgat tactgttgaa gacggtacag
     1021 gtttagaaga tgcattagac gtggttgagg gtatgcaatt tgaccgtggt tacttatcgc
     1081 catactttat caataaaccg gaagccggta cggttgagtt agaaaaccca tatattcttt
     1141 tagtggataa aaaagtgtct aacattcgtg aattattacc ggtgttagaa ggtgttgcaa
     1201 aagcaggtaa accgttatta attattgccg aagatattga aggcgaggcg ttagctacct
     1261 tagtggtgaa cactatgcgt ggtatcgtga aagtggcggc agtgaaagca cctggctttg
     1321 gtgatcgccg taaagcaatg ttacaagata ttgcgatctt aactgcaggt actgtgattt
     1381 ctgaagagat cggtatggag cttgaaaaag caacccttga agagcttggt caagcaaaac
     1441 gtgtggttat cagcaaagat aacaccacaa ttattgacgg tattggtgat gaaactcaaa
     1501 tcaaaggtcg tgtagcacaa atccgccaac aaatcgaaga ttcaacttca gattacgaca
     1561 aagaaaaact acaagagcgt gtagcaaaat tagccggtgg tgttgcggta attaaagtag
     1621 gtgcggcaac tgaagtggct atgaaagaga aaaaagaccg tgttgatgat gctcttcacg
     1681 caacccgtgc tgcggtggaa gaaggtattg taccgggggg cggagttgct ttagtgcgtg
     1741 cagccaacaa agtggctgat acactaaaag gcgataacga agagcaaaat gtaggtatca
     1801 aacttgcctt gcgtgcaatg gaggctccat tacgccaaat cgttgccaat gcaggtgaag
     1861 aagcctcagt tgtagcccgc aacgtgaaag aaggttcagg caactacggc tataacgcag
     1921 gtacagagca gtacggcgat atgcttgaaa tgggaatttt agacccaact aaagtgactc
     1981 gttcagcact tcaattcgcg gcttctattg caggtttaat gatcactaca gaatgtatga
     2041 ttaccgatct gccaaaagaa gagaaattag atcctgccgc aatgggcggt atggggggaa
     2101 tgggcggaat gatgtaattg cccattgagt gaacaaaagc tactgaaatt aattcagtag
     2161 cttttttgtt ggtaacaagt ggttaaattt atgctatttt ttgcaaataa aaaaccaagc
     2221 aagtaagctt ggttttagcg tttattgatg tttttgttta aggctgttta gtgctgttaa
     2281 acctttctca caactttttt gctgtaattc agaatccatt tttaggattt gttgctttgt
     2341 taaagaaagt ttatttttca tttttagcac ttgagtatga gtaccaggct gtctttctgc
     2401 atcagagacc aatttatttg ccgtgttaaa cattttctca cattcagttg gcaaattttt
     2461 acttaaagta ggttttgcaa ctgtaagtga tgaggtttta acacttgcta tagcattact
     2521 ggaaaccaat atggtacaaa gtgttattcc taaaattc
//
LOCUS       DANLRK010000238         2542 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.239, whole genome shotgun sequence.
ACCESSION   DANLRK010000238 DANLRK010000000
VERSION     DANLRK010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.239"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(72..990)
                     /locus_tag="PXA33_002478"
                     /pseudo
     CDS             complement(72..990)
                     /locus_tag="PXA33_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005700778.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(992..>2542)
                     /locus_tag="PXA33_002479"
     CDS             complement(992..>2542)
                     /locus_tag="PXA33_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase/relaxase domain-containing protein"
                     /protein_id="HDL5121769.1"
                     /translation="VHDIGKVVVDIEITLKDGSRWFAWNGLPPQPYKFKYIKGRDYHL
                     HPVLGGFLANQLIPKSAFDWLGQYPEVISSLMYAMSGHNDKAGLLSEIVQKADQHSVT
                     LAIGGDVNKLVQRPVNSLPKQIVLALRYLLEQKIKLNTPKGPADGWFTEEGLWLVSKT
                     AADNIRAYLLGQGISVPKDNPKLFDEMQSLGIVEESPAGGAIWSCRIQADSGWTPANT
                     FTLLRIKPEVAWENINDRPNYFAGKVKIVEQITEESNIETQSTLVDGTTKPAPLPSVQ
                     TGGIIASKEVEISQVETNSDNAPSSEIIINDENDLTSFVLDMFAPTESSEPSLDDISI
                     GKGQEIASMPSKQEVQDKSTTKKLKVTQSAVETPETLTLSELQLQNKENSITGDNFVK
                     WLKSGIATNKFAINKPTAKLHIVEDHLFLVTPVIFEMYLQETGQPYDKETINNLQYDF
                     QGLGIHSPKNTMTNGKPDTINFWKCDVIGPRKTSSLTGYLIKNTRLFFGDKVLLNNHC
                     LTLQKEET"
BASE COUNT          730 a          491 c          459 g          862 t
ORIGIN      
        1 tacggaacaa agaagatatt atttcataaa aattcgcccc aaaagggaga agtatctccc
       61 ttttgtattt tttataacaa attaaccgtt tctctcaaca tttccacatc gtgttcaata
      121 tatcctagtg tcacactgat atctttatgc cctaacagtt tctgaaccac ataaacattt
      181 tcgggcttct tcattaattg agtagcaact gtatgtcgaa atctgtgtgg tgatgaagta
      241 aaaccgacca agtcagaaat atgccgaaat atatgtgtaa gctgattttg gctcatcggc
      301 tgtcctcttc ttcgagagca tcgagaaaat aaattgagat tgaacaattg gcaattatct
      361 gtttgttttc ttatttttaa ttcagtaata agcctttcta tatgaatata aagtgaatct
      421 gaaatcggta cgatatggta atcatgattt ttattgattt ccggcgaaat ataaatcgtc
      481 ctattagaaa gatctacatc actaattttc agtttcacta attgtcctcg ccgaatcccc
      541 gtacatctca atgtcataat aattgcaagg acaaaccagc ttggttttag aaagtcaggt
      601 aatacaacgt tattattgag tatgtggtct attttatgta attgttcggt tgaataagtt
      661 ttctttctcc ttttcgattc tcgaataaat aactgattaa atggatttcg atttatatta
      721 agtaatcctt gcgatatacc aaagttatat aaggctttta aatgtctgac ataattattc
      781 cacgttactg ctttgatttt atcgccaaca atttgattac gccattcaat gagcatatcg
      841 gtggtaatgt cattggcatt taactttgga taacgctttt tgataattcg gattacgttg
      901 ttgtagctcc gtttggtatc cggacgaaga tagtgacgat agaaaaaaaa atcggtctag
      961 tagcgaatca aaagagatag aagttatcat tttaagtttc ctctttctgt aaagttaaac
     1021 agtgattatt taatagtacc ttatccccaa aaaagagacg ggtatttttt atcaaatatc
     1081 ctgttaaaga ggaagtcttt ctcggtccaa tgacatcaca tttccaaaaa ttgatggtat
     1141 ccggcttacc gttggtcatt gtgtttttag gtgaatgaat ccctaatcct tgaaaatcat
     1201 actggagatt attaattgtt tccttatcat aaggctgtcc agtctcttgc aaatacattt
     1261 caaaaataac gggggtaact aaaaacaggt ggtcttcaac aatatggagc ttggcagtag
     1321 gcttgttaat tgcaaatttg ttggtcgcaa tacctgattt gagccacttt acaaagttat
     1381 ctcctgtaat tgagttctcc ttattctgta actgtagctc tgatagtgtt aatgtttcgg
     1441 gcgtttctac tgcactttgt gttactttga gctttttagt ggtagattta tcctgtactt
     1501 cttgtttgga tggcatactt gcaatttcct gtcccttacc aattgaaata tcatcaaggc
     1561 ttggttcgga actttccgta ggtgcaaaca tatccagcac aaaggaggtt aaatcatttt
     1621 catcatttat gatgatttct gaggatggtg cattatccga gtttgtctct acctgagata
     1681 tttcaacttc tttagaggca ataatgcccc ctgtctgaac actcggtaat ggagctggtt
     1741 tagtggtccc atcgactaat gtagactgag tctctatatt actttcttct gttatctgct
     1801 cgactatttt aacttttccg gcaaaataat ttggtcgatc attaatattc tcccaagcca
     1861 cttctggttt aattctcaat aatgtaaagg tatttgctgg cgtccaacct gaatcagctt
     1921 gaatacggca actccaaatt gctccaccag ccggtgattc ttctacaata ccgagagact
     1981 gcatttcatc aaataacttt ggattatcct taggcactga aattccctgc cccagtaagt
     2041 aagctcggat attatcagca gcagttttgc taacaagcca caatccttcc tcagtgaacc
     2101 aaccgtctgc cggacctttg ggtgtattta actttatttt ctgttccagt aaataacgta
     2161 gagccagcac aatttgcttc ggcaatgaat taactggtcg ctgtaccagc ttattcacat
     2221 cgccaccgat tgctagcgta acactatgct gatcggcttt ctgcacgatc tcagataaaa
     2281 gcccggcttt atcgttatgc ccagacattg catacatcag tgaagaaatg acttctggat
     2341 actgtcccag ccaatcaaac gcagattttg gaataagttg atttgcaagg aagcctccta
     2401 gcaccggatg aagatggtag tctcgacctt tgatgtattt gaatttataa ggttgaggtg
     2461 gtaacccatt ccaagcaaac caacgtgaac cgtctttgag tgtaatttca atatcaacga
     2521 caactttgcc aatatcgtga ac
//
LOCUS       DANLRK010000239         2518 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.240, whole genome shotgun sequence.
ACCESSION   DANLRK010000239 DANLRK010000000
VERSION     DANLRK010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2518
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.240"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            58..2445
                     /locus_tag="PXA33_002480"
     CDS             58..2445
                     /locus_tag="PXA33_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HDL5121770.1"
                     /translation="MEKMMRKINQLIISPYFFLSILSASANSDFWKSDLNFNLTNITK
                     RVGVDNFTTPVAGEPWAGIGPNGEAAGTAPLYYSRIPAMQVTEDNKLVVMFDLRWNRA
                     YDQDRIDPGIAISSDGGHTWTKKTAWSFDRTNHPARRSMDPTLLHNPIDNSLYVMHGT
                     WSMSDQQWYGGRVPHFNSGSWAATIYKSTDGGLNWEKNTEFSKFSNLDVFSKVTKNGK
                     PTLGFLGGVGSGIVMQDGTLVFPIQTAHQNGIATTIMYSKDNGKTWEMPEIDNPVAPN
                     QSSLENMVFELEPGKLVMTGRGNSRWAYSSTDMGKTWELFTPTNGLLPTSSQPTQGSS
                     IYVTLPNGRKVLLISKPDGKNDGWKRGDITLWMLDAKDPSHKHKVHIIRPGSGNAAGA
                     GYSSLAYKEGNLFVAFENDGDISVKNLTEHIAEMEAKAIEWGLPDELTPALDKINALP
                     YLNKAQKETLVEKMRRANDTAIAQSFVINKEMYNLKNNSDELDKLAKNITKALPSQQN
                     IYQRALAYVNKVTNTADTTYLNYNGIMDTYANLYESYLALNTKLDFTRYIQKARKFNE
                     YNTDILYRSFDNFFAHYDTGVKHNNLSLGLNTKLSENLRGGLFFEYSNKNRNSVQIGA
                     RAKYEMDNHQLSGFLRYRGVKHKDMLARNNSVDGYLNYAYRIQLDDKLSISPSVGAYV
                     SRSSRSLIDEDLAINSRTVYASDVGLNIHYKLNDIDAYIRPSIGFVNGDITLSQSNDM
                     TNTYKIKDKSNVYSVTTGLKKRFANGVALGADFKLHRYGSQTTESNFGLNVSYNW"
BASE COUNT          825 a          444 c          487 g          762 t
ORIGIN      
        1 attgaaccat ataatagctg ttcagctaaa aattagtctt tgcactttat ccccctaatg
       61 gagaaaatga tgagaaaaat caatcagtta atcatatccc catacttttt cctaagtatt
      121 ttatctgctt ccgctaactc tgatttttgg aaaagcgacc ttaattttaa tctcacaaat
      181 attaccaagc gtgtcggagt cgataacttt accactccag tagctggcga gccttgggct
      241 ggtattggtc caaatggtga agctgcaggt actgcgccat tatattatag ccgtattcca
      301 gctatgcaag ttaccgaaga taacaaatta gttgttatgt ttgatttacg ctggaatcgt
      361 gcgtatgacc aagatcgtat tgatcctggt attgcgattt cttccgatgg tggccatact
      421 tggacgaaga aaaccgcttg gtcttttgat agaactaatc acccagcacg ccgttcaatg
      481 gatccaacgc tattacataa tcctattgat aatagtttat atgtaatgca cggaacttgg
      541 tctatgtcgg atcaacaatg gtatggtggg cgtgttcctc actttaatag tggcagctgg
      601 gcagctacca tttataaatc tactgatggt ggtttaaatt gggagaaaaa tactgagttt
      661 agtaaattca gcaatttaga cgtattttct aaagtcacca aaaatggaaa acctacctta
      721 ggctttttag gcggggtcgg atctggaatt gtaatgcaag atggtacgtt ggttttccca
      781 attcaaaccg cacatcaaaa tggtattgcg accactatta tgtattctaa ggataatggg
      841 aaaacttggg aaatgccaga aattgacaac ccagttgcac cgaaccaaag ttctttagaa
      901 aatatggtgt ttgaactcga accgggtaag ctagtgatga ctggacgagg aaacagtcgc
      961 tgggcatact cttctactga tatgggtaag acctgggaat tatttactcc aactaatgga
     1021 ttattaccaa ctagctcaca accaactcaa ggctcttcta tctatgtaac actaccaaat
     1081 ggtcgaaaag tgctactaat ctctaaacca gatggaaaga atgatgggtg gaaaaggggt
     1141 gatattacac tttggatgct agatgcaaaa gatccatcgc acaaacacaa ggtgcatatt
     1201 attcgtccag gttcaggtaa tgccgctggt gctggttatt cctctttagc ctataaagaa
     1261 gggaatttat ttgttgcttt tgaaaacgat ggtgatattt cagtaaaaaa tttaactgaa
     1321 catattgctg aaatggaagc aaaagctatc gaatggggat taccagatga acttactcct
     1381 gctttggata aaatcaatgc actgccttat ctcaataaag ctcaaaaaga gacgctagtc
     1441 gaaaaaatgc gtcgtgctaa tgatactgcg attgcacaat cttttgtgat taacaaagaa
     1501 atgtataatc tgaaaaacaa tagtgatgag ctggataaat tggcaaaaaa tattactaaa
     1561 gcactgccgt ctcaacagaa tatttatcaa agagccttag cctatgtgaa taaagtcacc
     1621 aatacagcgg ataccactta tttaaattat aatggtatta tggataccta tgctaattta
     1681 tatgaaagct atttggcttt aaatactaaa ttagacttca ctcgctacat ccaaaaagcg
     1741 agaaagttca atgaatataa cactgatatt ttatatcgta gctttgataa tttctttgcc
     1801 cattacgaca caggtgtaaa acacaataat ctatccttag gcttgaatac aaaattaagt
     1861 gagaatttac gtggtggctt attctttgaa tacagtaata aaaatcgtaa tagcgtacaa
     1921 attggtgcaa gagctaaata tgaaatggat aatcaccaac tttctggctt cttacgctat
     1981 cgtggcgtaa aacataaaga tatgctagca cgtaataaca gtgttgatgg ctacctaaat
     2041 tatgcttatc gtattcaatt agatgataaa ttaagcatta gcccatcagt tggtgcttac
     2101 gtttcacgtt cttctcgctc attaatagat gaagatcttg cgattaatag ccgcacggtc
     2161 tatgcaagtg atgtaggatt gaatatccat tataagctaa atgatattga tgcttatatt
     2221 cgtccaagta taggctttgt caatggtgat ataactttat ctcaatctaa tgatatgaca
     2281 aacacataca aaataaagga taaaagtaac gtgtattctg tcacgacagg gttgaaaaaa
     2341 cgctttgcaa atggtgtggc attaggggct gatttcaaat tacatcgtta tggttctcaa
     2401 actactgaaa gtaatttcgg tctaaatgta agttataact ggtaataccg taaactgatt
     2461 acgatttaaa agttagacta gcccaagact gagttctata ttccatagag ctcagtct
//
LOCUS       DANLRK010000240         2474 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.241, whole genome shotgun sequence.
ACCESSION   DANLRK010000240 DANLRK010000000
VERSION     DANLRK010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.241"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(116..191)
                     /locus_tag="PXA33_002481"
     tRNA            complement(116..191)
                     /locus_tag="PXA33_002481"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(156..158),aa:Thr,seq:ggt)
     gene            complement(195..269)
                     /locus_tag="PXA33_002482"
     tRNA            complement(195..269)
                     /locus_tag="PXA33_002482"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(234..236),aa:Gly,seq:tcc)
     gene            complement(313..397)
                     /locus_tag="PXA33_002483"
     tRNA            complement(313..397)
                     /locus_tag="PXA33_002483"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(361..363),aa:Tyr,seq:gta)
     gene            complement(432..507)
                     /locus_tag="PXA33_002484"
     tRNA            complement(432..507)
                     /locus_tag="PXA33_002484"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(472..474),aa:Thr,seq:tgt)
     gene            754..1704
                     /gene="coaA"
                     /locus_tag="PXA33_002485"
     CDS             754..1704
                     /gene="coaA"
                     /locus_tag="PXA33_002485"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HDL5121771.1"
                     /translation="MSLEKTSKITPFLTFDRQQWADLRKSVPLKLTEQDLKPLLGFNE
                     DLSLEEVRTIYLPLARLIQYYIEENLKRQTVLHRFLGIEPPKVPYIISIAGSVSVGKS
                     TSARILQALLSHYPKERKVDLITTDGFLYPLATLTEKNLLKKKGFPESYDIHSLIRFV
                     SDIKSGKRNVKAPIYSHLTYDIIPNQFNTVDQPDIVILEGLNVLQSGMNYPSSPHNVF
                     VSDFVDFSIYVDAEESLLKKWYINRFLKFRRSAFADPNSYFHHYASLSEQEAIETAST
                     IWEEINGLNLRQNILPSRERANLILIKGQDHAIETVKLRK"
     gene            1857..2396
                     /locus_tag="PXA33_002486"
     CDS             1857..2396
                     /locus_tag="PXA33_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HDL5121772.1"
                     /translation="MNKKFLLPLILFLALSVAFLVQLARNSQGDDPKKLESALIGKTV
                     PSFNLESVLDEKQTIDQNIVKTGKPRLLNVWATWCPTCYAEHEYLTILAKQGVEIVGI
                     DYKDERPKAIKFLANYGNPYKAIIFDPKGSLGLDLGVYGAPETFIIDGNGVIQYRHAG
                     DVNDQVWNQVLKPIYDGLK"
BASE COUNT          780 a          512 c          468 g          714 t
ORIGIN      
        1 tttctaaaag cctcttaaac aaaaacggtt acaatggaac aaaagaaccc attaaccata
       61 atgtagaaat taataccaat aaaaatttgc gtgagaaaga tagaattcag agaagtggtg
      121 ctgataggcg gatttgaacc gccgacctca cccttaccaa gggtgcgctc taccaactga
      181 gctatatcag cgcttggagc gggcagcggg aatcgaaccc gcatcattag cttggaaggc
      241 taaggtaata gccattatac gatgcccgct gttctaaatt cgtctgacct acgcgattca
      301 aaatatcaga gatggtggag ggagaaggat tcgaaccttc gaaggctgag ccaacagatt
      361 tacagtctgc cccctttggc cgctcgggaa cccctccacg ataattgaat acttatttac
      421 gcctgagtta atggtgccga ctaccggaat cgaactggtg acctactgat tacaagtcag
      481 ttgctctacc tactgagcta agtcggcgtt gctgtgtcgt aagcaagtga tgtgcattat
      541 agggaaattt taacagcgtg caagtgtttt ttaaaaaaat attaaaaaaa gcgttctttc
      601 gatttttaaa tatacaaaat gactattttt tgtaaaatac aacggatttt ctctgcataa
      661 ctacggcaaa ataagtgcat atattgtata gttacgccct taatttggta tttaattacc
      721 ttaatattcc agcaaaaaat tgagattttg gctatgagcc ttgaaaaaac cagcaaaatt
      781 actccttttc tcacttttga tcgccaacaa tgggctgatt tacgcaaatc cgttccattg
      841 aagctgaccg agcaagacct aaaaccgctt ttaggcttta atgaagatct ttcattagaa
      901 gaagtacgca ctatttattt accgcttgcc cgtttaattc aatactatat agaagaaaac
      961 ttaaaacgcc agacagtctt acatcgcttt ctcggcattg aaccgccaaa agtcccttac
     1021 attattagta ttgccggcag cgtatcggtt gggaaaagta cctctgcccg tattctgcaa
     1081 gctctgctct cccattaccc taaagagcga aaagtggatt taattaccac cgacggtttt
     1141 ttatatccac ttgcaacgct aaccgaaaag aacttactca agaaaaaagg ctttccggaa
     1201 tcttatgata ttcacagcct gattcgcttt gtatctgata ttaaatccgg caaacgcaat
     1261 gtgaaagctc cgatttattc acatttgacc tacgatatta tcccaaatca atttaatacg
     1321 gtggatcaac ccgatattgt gattttagaa gggctaaacg tgttacaaag tggaatgaac
     1381 tacccgtcca gcccacataa tgtatttgta tcggactttg tggatttttc tatttatgtg
     1441 gatgccgaag aaagtttgct gaaaaaatgg tatatcaacc gtttcttgaa attccgccgc
     1501 agtgcctttg ccgacccaaa ctcctatttc caccattatg cgagtttgtc cgagcaagaa
     1561 gcgattgaaa ccgcctcaac gatttgggaa gagatcaacg gcttaaattt acgccaaaat
     1621 attctcccaa gccgagagcg tgccaacctc attttaatca aagggcaaga ccacgccatt
     1681 gaaacggtaa aattacgcaa ataatatttt gcaaataatt gggctttttc gaccgcttgt
     1741 aataccttaa aatccgttga ataccactca acggattttt tatttaaatc aatgcttatt
     1801 caactatttt ttggtaagct aacgactttg agagtgattg atattaggca tatacaatga
     1861 acaaaaaatt tctacttcct ttaattctct ttttggcgtt atctgtggca tttttggttc
     1921 aactcgctcg caactcgcaa ggcgatgatc cgaaaaaatt agaatcagcc ttaattggca
     1981 aaacagttcc gagctttaat ttagaatcgg tattagatga aaaacagaca atcgaccaaa
     2041 atatcgtcaa aaccggcaaa cctcgcctgc ttaacgtgtg ggcaacttgg tgtccgactt
     2101 gttatgccga acacgaatac ttaaccattt tggcaaaaca aggggtggaa attgtcggta
     2161 ttgattataa agacgaacgc ccaaaagcga ttaaattctt agccaattac ggcaaccctt
     2221 ataaagcgat tatttttgac ccgaaaggct cactcggttt agatttaggt gtttatggtg
     2281 cacccgaaac ctttattatt gacggcaacg gcgtgattca ataccgccac gccggcgatg
     2341 taaacgacca agtgtggaac caagtgctta aaccgattta tgatggatta aagtaaatgt
     2401 ttgtatttcc tgctgacatt aagaaatcta taaaaagcaa ttatcaaaga gaatataact
     2461 ttatttttat aata
//
LOCUS       DANLRK010000241         2396 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.242, whole genome shotgun sequence.
ACCESSION   DANLRK010000241 DANLRK010000000
VERSION     DANLRK010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.242"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            35..841
                     /locus_tag="PXA33_002487"
     CDS             35..841
                     /locus_tag="PXA33_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005726415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HDL5121773.1"
                     /translation="MALAVAACGNLSKVSDEGTTENPVFPKISESEFNHDGSQFGSWP
                     NWENVRQIEKGMNKDQLYNLIGRPHFEEGLYGVREWDYAFNYRENGVHKICQFKILFD
                     KNMNAQSFFWYPNGCNGNSSFALTGDFLFDFDKATLTTKGKEVVDNVAQQLKASKAQQ
                     VKVAGYTDRLGSAAYNLDLSQRRSNTVKARLVQQGVTAQIEAVGYGKANQVKACDGET
                     GQALKDCLRPNRRVEISANCGVMKQNEGGNVAGPNGPAPLYQTPAYDGSK"
     gene            929..1747
                     /locus_tag="PXA33_002488"
     CDS             929..1747
                     /locus_tag="PXA33_002488"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphatase"
                     /protein_id="HDL5121774.1"
                     /translation="MNYQAIAFDLDGTLLSSNATILESSKEAIKKVREKGIKVYFVTG
                     RHHTAVRPYYVELGLDTPVVCCNGTYVYDFKNEKVLSGNPLSAEIASKLVNDAQAQGI
                     HTAVYFEDAMTYESLNIHFTKFQKWVESCAPEVRPNVYQVENFQQEIDKGVTVWKVLI
                     SDPDLAKMQKFVEKLPPDQVSPEWSWVDRVDITSVGNSKGARLAEVLKTEGIDLQKVI
                     AFGDNFNDVSMLETVGLGIAMGNSEPDIQQKAKKTIGSNNEDSIANELKAQFNL"
     gene            1804..>2396
                     /locus_tag="PXA33_002489"
     CDS             1804..>2396
                     /locus_tag="PXA33_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili methyl-accepting chemotaxis
                     transducer N-terminal domain-containing protein"
                     /protein_id="HDL5121775.1"
                     /translation="MIGFASIISGISLGIMWSNKSDASLINVSGSLRMQSYRLLSEMD
                     SKKALLPERLLEYHRTLHATELSSLKQSVLLPDSVIESYQQLLKDWYEMQSYVAQGDK
                     VNYASHIEDYVQRVDDFVFRLQEFAELKLKIATGVIAIAMLLIIALAYIGVWYTRKKI
                     IGPLRQLVKASWQIQNKDFDHVKLAVNEPNELGLLSST"
BASE COUNT          794 a          372 c          499 g          731 t
ORIGIN      
        1 aaaagccctt ttccgtggat ttttattatc gacagtggca ttagcagtgg ctgcttgtgg
       61 taacttaagt aaagtgagtg atgaaggaac aactgaaaac ccggtattcc cgaaaatctc
      121 agaatctgaa tttaaccacg atggttcaca atttggttca tggccaaact gggaaaacgt
      181 tcgtcaaatt gaaaaaggga tgaacaaaga tcaattatat aatctaattg gtcgtcctca
      241 ttttgaagaa ggcttatatg gtgttcgtga atgggactat gcattcaatt accgtgaaaa
      301 tggcgtacat aaaatttgcc aatttaaaat tttattcgat aaaaatatga atgcacaaag
      361 tttcttctgg tatccaaatg gatgtaatgg aaactcatca tttgctttaa ctggcgattt
      421 cttattcgat ttcgataaag cgactttaac tacaaaaggt aaagaagttg ttgataatgt
      481 tgctcaacaa ttaaaagcat ctaaagcaca acaagttaaa gtagcaggct atacagaccg
      541 tttaggctct gctgcttata acttagactt atcacagcgt cgttctaata cagttaaagc
      601 tcgtttagta caacaaggcg ttacagcaca aattgaagcg gtaggttatg gtaaagcgaa
      661 tcaagtaaaa gcttgtgatg gtgaaactgg tcaagcatta aaagattgtt tacgccctaa
      721 ccgtcgtgtt gaaatttctg caaattgtgg tgtaatgaaa caaaatgaag gtggcaatgt
      781 tgcaggtcct aatggtccgg ctccacttta tcaaacccca gcatatgatg gcagtaaata
      841 atttctagtt ttgactaact tagagtagaa tattaaggca accaaatggt tgccttttct
      901 tttatctatt tatttatagg ggaatactat gaactaccaa gctattgcat ttgatttaga
      961 cggaacacta ctttcatcga acgcaacaat tttagaatcc agtaaagagg ctattaaaaa
     1021 ggtaagagaa aagggaatta aagtctactt tgttaccggt aggcaccata ctgcggttcg
     1081 cccttattat gtagaactag ggctggatac gccagttgtg tgttgcaatg gcacttatgt
     1141 gtatgatttt aaaaatgaaa aagtattaag cggtaatccg ttatcagcag aaattgcttc
     1201 aaaattagtt aatgatgcac aagctcaagg gattcatact gcggtttatt ttgaagatgc
     1261 aatgacttat gagtctttga atattcactt cactaaattc caaaaatggg ttgagtcttg
     1321 cgcaccagaa gttcgtccga atgtttatca ggtagaaaat ttccaacaag aaattgataa
     1381 aggtgtaacc gtatggaaag tgcttattag cgatccagat cttgccaaaa tgcaaaaatt
     1441 tgttgaaaaa ttaccgcctg atcaagtaag cccggaatgg tcttgggttg atagggttga
     1501 tattaccagt gttggtaaca gcaaaggggc aagattagcg gaagtattaa aaacagaagg
     1561 tattgattta caaaaggtga ttgcctttgg cgataatttc aatgatgttt ctatgctaga
     1621 aaccgttgga ttaggcattg caatgggaaa ttcagaacca gatattcagc aaaaagcgaa
     1681 gaaaactata ggttcaaata atgaagatag tattgctaat gaattgaaag ctcaatttaa
     1741 tctttaatta tgataaaagc aaaatactct attgctaggc ggataggact ctatttttta
     1801 attatgatcg gatttgcttc gattatcagt ggaatctcac ttggtattat gtggagcaat
     1861 aaatctgatg cgagcctgat taatgtttca ggctcacttc gtatgcaaag ttatcgattg
     1921 ttatcagaaa tggattcaaa aaaagcgctg ctacctgagc gattgttgga atatcatcga
     1981 acgctacatg ctacagagct tagttcatta aagcaaagtg ttttattgcc agatagcgtg
     2041 atagaaagct atcaacaatt acttaaagat tggtatgaaa tgcaatctta tgtcgctcag
     2101 ggagataaag ttaattatgc ttcgcacatt gaagattatg tgcagagagt tgatgatttt
     2161 gttttccgct tacaagagtt cgctgagtta aaattaaaga ttgcaacagg cgtgattgcg
     2221 attgcaatgt tactgattat tgcccttgct tatattggcg tttggtatac tcgaaaaaag
     2281 attattggac ctttaaggca attggtaaaa gcgagttggc aaattcaaaa taaagatttt
     2341 gaccacgtaa aattagcggt taatgaaccc aatgaattag ggctactttc ctctac
//
LOCUS       DANLRK010000242         2388 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.243, whole genome shotgun sequence.
ACCESSION   DANLRK010000242 DANLRK010000000
VERSION     DANLRK010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2388
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.243"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            4..495
                     /locus_tag="PXA33_002490"
     CDS             4..495
                     /locus_tag="PXA33_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014391214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HDL5121776.1"
                     /translation="MGLDKEIAKKLADELNGLLATYQVFYTNVRGYHWNVRGVNFFEL
                     HAKFEEIYDDLVTKVDEIAERILTLGHTPQNAFSQYLTKSQIQEHIGVSAAQECLRGT
                     LNGFKTLLGQQREILALANDANDEGTASQMSDYIKEQEKLVWMFTAACAECSDYAQGC
                     SCN"
     gene            554..1267
                     /locus_tag="PXA33_002491"
     CDS             554..1267
                     /locus_tag="PXA33_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HDL5121777.1"
                     /translation="MAMSQGFQFKQFFIRHDRCAMKVNTDGILLGAIAHIKDCQNILD
                     LGIGSGLVALMLAQRTDDCCHITALELEPNAYQQAVENAQNSAWANRISVLQGDVMQQ
                     AFEQKFDLIVSNPPYFADSLAARTTERDLARSISHYSHLNWLAKAKPWLAENGKITLI
                     LPIAAAEKLVDQSEQIGLYCVEQWLIFTKSGKLAKRSIVSFSLNKQKREHKELIIYDL
                     DNCYTTEFKRLTQDFYLNF"
     gene            complement(1378..2382)
                     /locus_tag="PXA33_002492"
     CDS             complement(1378..2382)
                     /locus_tag="PXA33_002492"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005651072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HDL5121778.1"
                     /translation="MAFNLKNRHLLSLVNHTEREIRFLLDLARDLKRAKYAGTEQQKL
                     KGKNIALIFEKTSTRTRCAFEVAAYDQGAHVTYIDPNSSQIGHKESMKDTARVLGRMY
                     DAIQYRGFKQSVVQELADYAGVPVFNGLTDEFHPTQMLADVLTMIENCEKPLSQISYV
                     YIGDARNNMGNSLLLIGAKLGMDVRICAPKALLPEDAFVKMCEKFAAESGARMTVTED
                     IETAIKGVDFVHTDVWVSMGEPLESWTERIDLLMPYRVTPELMQATDNPKVKFMHCLP
                     AFHNSETKVGKEIAEKYPALANGIEVTEEVFESPMNVAFEQAENRMHTIKAVMVASLA
                     "
BASE COUNT          707 a          476 c          468 g          737 t
ORIGIN      
        1 aatatcggtt tagataaaga aatcgcaaaa aaattagcag atgagttaaa tggtttatta
       61 gctacttacc aagtgtttta cactaatgta cgtggctacc attggaatgt acgtggtgtg
      121 aacttctttg aattacatgc aaaatttgaa gaaatctacg atgatttagt gacaaaagta
      181 gatgaaattg cagaacgtat tttaacctta ggtcacacac ctcaaaatgc gttcagccaa
      241 tatttaacca agtcacaaat tcaagaacat attggtgttt cagctgcaca agaatgttta
      301 cgtggtactt taaatggttt caaaacatta ctgggacaac aacgtgaaat tcttgctcta
      361 gcaaatgatg caaacgatga aggtactgct tctcaaatga gtgattacat caaagaacaa
      421 gaaaaattag tatggatgtt cactgcagct tgtgctgaat gttcagacta cgcacaaggt
      481 tgttcttgta actaagttgc aataatataa ttaacccccg ataatgaaag attatcgggg
      541 gttacttttt cagatagcta tgtcgcaagg atttcaattt aaacagtttt ttattcggca
      601 cgacagatgt gcaatgaaag tcaatacaga tggtattcta ttgggagcga ttgctcacat
      661 aaaagattgt cagaatattt tagatctggg tataggttct ggtttagtag cacttatgct
      721 tgctcagaga acagatgatt gttgtcacat tactgcgttg gaacttgaac caaatgccta
      781 ccaacaagcg gtcgaaaatg cccaaaattc tgcatgggca aatcggattt ccgttttgca
      841 aggtgatgtg atgcagcagg catttgaaca gaaatttgac ttgattgtta gtaacccacc
      901 ttattttgca gattcacttg cagctcgaac cacagagaga gatttagctc gctcaattag
      961 ccattacagc cacttgaatt ggctggcaaa agcaaaacca tggttagcag agaacgggaa
     1021 aattacactg attttaccta tagcagctgc agaaaaatta gttgaccaaa gtgaacaaat
     1081 tggattgtat tgtgttgaac aatggttaat ttttaccaag agcggcaaat tggctaaacg
     1141 cagtatagtg agttttagtt taaacaagca aaagcgtgaa cataaagagc tgatcatcta
     1201 tgatctagat aattgttaca ctactgagtt taaaagactt acccaagatt tttatttgaa
     1261 tttttaattt actccccggg ttagtgtttt ccacaaatct tcttcagcta cgatgatcta
     1321 gccttaagct aaaaagcaga ttaccaccca agataatccg ctttttagac ttagctatta
     1381 tgccaaactt gctaccatta ccgctttaat agtgtgcatt cgattttctg cttgctcgaa
     1441 tgccacattc attggcgatt caaacacctc ttcggttact tcaatgccgt ttgccagtgc
     1501 cggatatttt tcggcgatct ctttaccgac tttagtttca ctgttgtgga acgccggtaa
     1561 gcagtgcata aatttcactt tcgggttgtc ggttgcttgc attaattcag gcgttactcg
     1621 gtaaggcatt aataaatcta tacgttcagt ccagctctct agcggttcac ccattgaaac
     1681 ccaaacatca gtatgcacaa aatcgacacc tttaattgca gtctcaatat cttcggttac
     1741 cgtcattctt gcccccgatt cagctgcaaa tttttcacac attttcacga atgcatcttc
     1801 cggtaataag gctttcgggg cacaaattcg cacgtccatc cccaatttcg ccccaatcaa
     1861 caggagtgaa ttacccatat tattgcgagc atcgccgata tagacataac tgatttggct
     1921 aagcggtttt tcgcaatttt cgatcatcgt tagcacgtca gccaacattt gggttgggtg
     1981 gaattcatcg gttaagccgt taaacaccgg cacgccggca taatcggcaa gctcttgtac
     2041 taccgattgt ttaaagccgc gatattgaat cgcatcatac attctgccta gcactcgggc
     2101 ggtatctttc attgattctt tatgcccgat ttgcgaggag ttcgggtcaa tataggtaac
     2161 gtgagcccct tgatcgtaag ctgctacttc aaaggcacaa cgagtacggg tcgaggtttt
     2221 ctcaaaaatt aaggcaatat ttttgccctt taatttttgc tgctcagtgc cggcatattt
     2281 cgcccgtttt aaatctcttg ctaaatccaa caaaaagcga atttcacgct cagtgtggtt
     2341 tactaaactg agtaaatgtc tgttttttaa gttaaatgcc attttgtt
//
LOCUS       DANLRK010000243         2338 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.244, whole genome shotgun sequence.
ACCESSION   DANLRK010000243 DANLRK010000000
VERSION     DANLRK010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.244"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            40..1635
                     /locus_tag="PXA33_002493"
     CDS             40..1635
                     /locus_tag="PXA33_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HDL5121779.1"
                     /translation="MQRHEQLAQVQQTQKWDFIIIGGGASGLGIAVDAASRGYKTLLL
                     ESHDFAKGTSSRSTKLVHGGVRYLANGDVALVREALRERGRLAKNAAHLFKNQSFIIP
                     NYKFKDCVMYRVGLGLYDMLSGNLSLGKTENISKTEAKRRLPTLNDTGLRTGTVYQDG
                     QFDDSRLAVNMAQTVVEHGGTVLNHAKVTSLHKTPQGKIDGVTFYDELNQQEHTVFGT
                     AVINATGVFMNDILSMDHGSTKKFVVPSQGIHLVLDKSFLPSNDALMIPKTSDGRVLF
                     AVPWHEVLVVGTTDTLIENPSYEPIPLEQEVNFILETAAQYLTKKPTRDDVKAVFAGQ
                     RPLAAPEKEGQSTKEVSRSHKVLTDATSGLITITGGKWTTYRQMAEDTVEEALKVHSE
                     LGNKPCVTVNLPIHGNIPAAQVNLQDHLYVYGSDIPQLRALMTETPEYAQKIHPRLDY
                     TVAEVIWAVRHEMAQTVEDVLARRVRLLFTDARAAIDSAAKVAAIMAKELGKDEMWQQ
                     QQVIQFLDVAKHYLLVDYQPQPI"
     gene            complement(1722..2021)
                     /locus_tag="PXA33_002494"
     CDS             complement(1722..2021)
                     /locus_tag="PXA33_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121780.1"
                     /translation="MENIKMEELKLTEKELLVLKGNALRMRFKAETNKTKNEIMQPFK
                     QVKQTGLALVNSPAVKTIALDFLVKRLLSMKGISFSVLGLTAFFLLQQKHKRDDS"
     gene            complement(2008..>2338)
                     /locus_tag="PXA33_002495"
     CDS             complement(2008..>2338)
                     /locus_tag="PXA33_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDL5121781.1"
                     /translation="RGEMARIEINEQKNQLISVLIFILLAFVFLLVSFVSLLFGLDNY
                     LLPEQKVKVFFSIAIIMFIAVTICGMMILSALKKQRHFMQSTLTELKLDIAAFKSALT
                     LKQNGEH"
BASE COUNT          773 a          441 c          455 g          669 t
ORIGIN      
        1 aatgattaaa tgttttaatt cgagcaaaag gagcaaaaaa tgcaaagaca tgaacagcta
       61 gctcaagtac aacaaacgca aaaatgggat tttattatta tcggtggtgg cgcaagtggt
      121 ttggggattg cggtagatgc agcttcccgt ggctataaaa ccttattatt agaaagccat
      181 gactttgcga aaggtacatc gagccgaagc acaaaattag tccacggtgg cgttcgctat
      241 cttgcaaatg gcgatgttgc actcgtgaga gaagcattgc gtgaacgagg tcgtttagct
      301 aaaaatgcag ctcatttatt taaaaaccaa agctttatta ttccaaacta caaattcaaa
      361 gactgtgtaa tgtatcgtgt tggtttaggc ttatacgata tgctttcagg caacctgagt
      421 ttaggaaaga ctgaaaacat cagtaaaact gaagcgaaaa gacgtttacc aaccttaaat
      481 gatactggtt tacgtacagg cactgtttat caagatgggc aatttgatga ctcacgtctt
      541 gcagtgaata tggcacaaac ggtagtagag cacggcggta cagtactaaa tcacgccaaa
      601 gtgacttcac tgcacaaaac gccacaaggc aaaattgatg gggtgacttt ctacgatgaa
      661 ctcaatcaac aagaacatac tgttttcggc actgcggtga ttaacgcaac cggcgtattt
      721 atgaatgata ttttaagtat ggatcacggt agcacgaaaa aatttgtcgt accaagccaa
      781 gggattcact tagtattaga taaatcattc ttaccaagta atgatgcgtt aatgattcca
      841 aaaacctctg acggacgagt gttatttgct gtaccttggc acgaagtgtt agtcgtaggg
      901 acaacagata ctctgattga aaatccttct tatgagccaa ttccattgga acaagaggtg
      961 aactttattt tagagacagc cgcacaatat ctgacaaaaa aaccaactcg tgatgatgta
     1021 aaagcagtct ttgccggtca acgtccgctt gcggcaccgg aaaaagaagg tcaaagcact
     1081 aaagaagtat ctcgtagtca caaagttctc actgatgcaa ccagcggttt gattacgatt
     1141 acaggtggta aatggacaac ttatcgtcaa atggcagaag ataccgtaga agaagcgtta
     1201 aaagtccact ctgaactagg caataaacct tgtgtgaccg tcaacttacc gattcacggt
     1261 aatattccgg cagcacaagt gaatctacaa gatcatctat atgtatatgg ctcagatatt
     1321 ccacagttac gcgccttaat gactgagacc ccggaatatg cacaaaaaat ccatccgaga
     1381 ctagattata ccgttgcaga agtgatctgg gcagttcgcc acgaaatggc acaaacggtt
     1441 gaggatgtat tagctcgccg tgttcgtttg ctcttcactg atgcgagagc ggcgattgat
     1501 agtgcagcaa aagtggctgc tattatggcg aaagaattag gtaaagatga aatgtggcaa
     1561 caacagcaag ttatccagtt ccttgatgtt gcgaagcact atttattggt agattatcag
     1621 ccacagccaa tctaatacgc taaaagcaag cggttaaaat cgataaaaat attgcaatta
     1681 cgttatttgc aactttttaa accattttaa ccgcttgtat attagctatc atctctttta
     1741 tgtttctgtt gtaataaaaa gaatgctgtt aaccccaata cagaaaagct aattcctttc
     1801 atactcaata atctttttac caaaaaatct aatgcaatgg ttttaactgc cggtgaattg
     1861 accagtgcaa gccctgtttg tttcacttgt ttaaaaggct gcataatctc atttttggtt
     1921 ttattggttt ccgctttaaa tcgcattcta agtgcattac cttttagaac tagaagttcc
     1981 ttttcggtta atttaagctc ttccatttta atgttctcca ttctgtttca aagttaaagc
     2041 actcttaaat gcggcaatat ctaattttag ctcggttaat gtactttgca taaaatggcg
     2101 ttgttttttc aaggcagaaa gtatcatcat tccgcaaata gtcacagcaa taaacataat
     2161 aatggcaata gaaaagaata ctttaacttt ctgttcgggt aacaaataat tatctaaacc
     2221 aaataataaa ctcacaaaag aaactaataa aaatacaaaa gccaataaga taaatataag
     2281 tactgaaatt aattgatttt tttgctcatt aatttcaatt cttgccatct caccacgc
//
LOCUS       DANLRK010000244         2236 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.245, whole genome shotgun sequence.
ACCESSION   DANLRK010000244 DANLRK010000000
VERSION     DANLRK010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.245"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            <1..108
                     /locus_tag="PXA33_002496"
     CDS             <1..108
                     /locus_tag="PXA33_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1040 family protein"
                     /protein_id="HDL5121782.1"
                     /translation="KMRNAGKFEPIPGIKKDYEDDFKTALLRARGILND"
     gene            172..810
                     /locus_tag="PXA33_002497"
     CDS             172..810
                     /locus_tag="PXA33_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HDL5121783.1"
                     /translation="MKKLALKTAFVALSALFTFNSTAIAADPTAGKEYIEVRKAPSAQ
                     KEVVEFFSFYCPHCYDFELSYKIPSQIKEKLPSDSKLVQYHVNFLGRQSEDLTRAWAL
                     AMALGAEDKVKTALFEGAQKDAFKSMDDIRSVFLANGITAEQFDSGINSFAVNGLVNK
                     QVQLAEDFQIRGVPAFFVNGQYQLNLEGFADSSSTNDFIKRYVDAVVFLSKK"
     gene            894..1670
                     /locus_tag="PXA33_002498"
     CDS             894..1670
                     /locus_tag="PXA33_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HDL5121784.1"
                     /translation="MTKLSILNLVPIREGQTAKQAVDSMVKLAKHAESIGIERYWIAE
                     HHNMKNLASSATQLLIQHALSHTEKLRVGSGGVMLPNHSPYIVAEQYGTLETLYPNRV
                     ELGLGRAPGTDMRTATALRRNAQSLPFPDEISELKGYFEGTNSVSAYPAQGLNVPFYI
                     LGSSPESAYLAAERGLPYAFASHFAPRFMDDAVRIYRTHFKPSEVLDKPYVILGVNAI
                     AAETDQEAEQLATTQTQFFLNVVTNAQNYLQPPVESDDKV"
     gene            1680..1934
                     /locus_tag="PXA33_002499"
     CDS             1680..1934
                     /locus_tag="PXA33_002499"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase"
                     /protein_id="HDL5121785.1"
                     /translation="MKAEIVPHFGPVSFQQNALYNQEKPVVRQMTACSLIGSRESIEQ
                     QLKSLKARIEIDEIMAVSYIFDEQKQHQSYTWLKEIADKV"
     gene            1936..2229
                     /locus_tag="PXA33_002500"
     CDS             1936..2229
                     /locus_tag="PXA33_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HDL5121786.1"
                     /translation="MSKTMQQKAADAHNMCKLKGDSMLDNSDRQIAFEAVYDSEEIAV
                     QAVEFFTQKAKSVETEPCDIQSEITPIIDGFLMKMWITFSCQAEVILFQMAVR"
BASE COUNT          717 a          446 c          456 g          617 t
ORIGIN      
        1 aaaatgcgaa atgcaggcaa atttgagcct attcccggca taaaaaaaga ttatgaagat
       61 gattttaaaa cggctttatt gcgagctcgt ggaattttaa acgattaaga ttgtcttatc
      121 cgttagctag tttagcattt attatttatc aacatttttg aaggaatcat tatgaaaaaa
      181 ttggcattaa aaacggcatt tgttgcactt tctgcccttt ttacatttaa ctcaacggca
      241 atcgctgctg atccaaccgc aggcaaagaa tatatcgaag tgcgtaaagc accttctgca
      301 caaaaagaag tggtggaatt tttctcattc tactgcccac actgttatga ttttgaatta
      361 agctacaaaa tcccaagcca aatcaaagaa aaattaccaa gcgatagcaa attagtgcaa
      421 tatcacgtta atttcttagg tcgccaatct gaagatttaa ctcgtgcttg ggcattagca
      481 atggcattag gtgcggaaga taaagtaaaa accgcattat ttgaaggtgc tcaaaaagac
      541 gcattcaaat caatggacga tattcgctct gtattcttag caaacggcat tacagctgaa
      601 cagtttgata gcggtattaa tagctttgcg gtaaatggct tagtgaataa acaagttcaa
      661 ttagcggaag acttccaaat tcgtggtgtg cctgcattct tcgtaaatgg tcaataccaa
      721 ctcaacttag aaggctttgc agattcttca tcaacgaatg atttcatcaa acgttatgtt
      781 gatgcggttg tgttcttaag caagaaataa ccccttaaaa tagttagggt ttttacaatg
      841 ctgagtaggt aaatcctatt cagcattttt tatatgcaaa ctcggagaac aaaatgacaa
      901 agttatctat cctaaacctt gtacctattc gtgaagggca aactgccaaa caagcggtcg
      961 attctatggt gaaattagca aaacacgcgg aaagtatcgg cattgaacgc tattggattg
     1021 cagagcacca caatatgaaa aaccttgcca gctcggcaac acagctcctc attcagcatg
     1081 ccctttctca cactgaaaaa ttaagagtgg gatcaggtgg cgtgatgttg cctaaccata
     1141 gtccttatat tgtggcagag caatacggta cgcttgaaac actctaccct aaccgtgtag
     1201 aacttgggct tgggcgagca ccaggcaccg atatgcgaac cgctaccgct ttacgccgta
     1261 atgcacaaag tctgccattt cctgatgaaa tcagcgaact aaaaggttat tttgaaggca
     1321 caaattctgt gtctgcctac cctgcacagg ggttgaatgt gccgttttat attttaggct
     1381 caagccctga aagtgcctat ttagcagcag aacgtggctt gccttatgcc tttgcttcac
     1441 atttcgctcc tcgttttatg gacgatgcgg tgcgaattta tcgcacacac tttaagccgt
     1501 ctgaagtgtt agataaaccc tatgtgattt taggtgtgaa tgcgattgcg gcagaaaccg
     1561 accaagaggc agaacagctt gctaccactc agactcagtt tttcttaaat gttgttacca
     1621 atgcccaaaa ctatttacaa ccgcccgtcg aatccgatga taaagtgtga aaaaattttg
     1681 tgaaagcgga aatcgtaccg catttcgggc ctgtttcgtt ccaacaaaat gcgttataca
     1741 accaagagaa acccgttgtt cgccaaatga ctgcttgttc acttatcggt agccgagaga
     1801 gcattgaaca acagctcaag tcattaaaag cacgcattga aattgatgaa attatggcgg
     1861 taagctacat tttcgatgag caaaaacagc atcaatccta tacttggtta aaagagattg
     1921 cagataaggt gtgatatgag caaaacaatg caacaaaaag ccgctgatgc acataatatg
     1981 tgtaagctaa aaggcgactc tatgttagat aactcagacc gccaaattgc ctttgaagca
     2041 gtgtatgata gtgaagaaat agctgtacaa gcggtcgaat ttttcacaca aaaagcaaaa
     2101 tcggtagaaa ccgagccttg cgacattcaa agtgaaatca cacccattat agatggcttt
     2161 ctgatgaaaa tgtggataac tttcagttgc caagcagaag taatcttatt tcaaatggcg
     2221 gtgagataga tataaa
//
LOCUS       DANLRK010000245         2192 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.246, whole genome shotgun sequence.
ACCESSION   DANLRK010000245 DANLRK010000000
VERSION     DANLRK010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.246"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            <1..1140
                     /locus_tag="PXA33_002501"
     CDS             <1..1140
                     /locus_tag="PXA33_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015702017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HDL5121787.1"
                     /translation="SLISACSSLPTSGPSYSEILASNENTAETQLPEVNLIKLDNITV
                     QNLYQEQQSQRFSGFDGSMGVGGYAGTVNVGDILEISIWEAPPAVLFGGTFSAEGQGN
                     GHLTQLPQQMVNKNGTVTVPFIGNIKVAGKTPEVIQNQIVGALSRKANQPQALVRIAN
                     NLSSDVTVIRQGNSIRMPLSANNERILDAVAAVGGTSENIEDVTVRLTRGNKVRSMAF
                     ETLIADPSQNITLRSGDVIALMNTPYSFTGLGAVGNNQQMRFSSKGITLAEAIGKMGG
                     LIDERSDPRGVFVFRHIPFTQLDYDNQAQWREKGYAEGMDVPTVYQVNLLKPQSMFLL
                     QRFPIQDKDIVYVSNAPLSEFQKFLRMIFSITTPITGTANTIRAY"
     gene            1212..>2192
                     /locus_tag="PXA33_002502"
     CDS             1212..>2192
                     /locus_tag="PXA33_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis protein"
                     /protein_id="HDL5121788.1"
                     /translation="MSKKKKHFLANPLLWLTVIIPTACSGIYYSLFASDIYVSESSFV
                     VRSPNNQSSLTGVGALLQNSGFARSQDDTYSVQEYMRSRTALEQLEAEIPVKRFYSEQ
                     GDLLSKFNGLGFNDTQEAFYRYFKERLNIDVDSISGIATLRVRAFNAEEGQKINENLL
                     QQGETLINRLNERARQDTVEFAEERVKEAEKDVNNTALALSQYRTKNKIYDLPAQSSV
                     QLSLISTLKSELIRVETQLAQLKSITPDNPQVAALQMRQKSLKKEIDDQSKQLAGGNT
                     SVATQTAEYQRLVLANELAQQQLAAAMTSLQNTRGEADRQQLYLEVIGRPS"
BASE COUNT          718 a          396 c          435 g          643 t
ORIGIN      
        1 tcattaattt cagcctgctc tagcctacca acgtccggcc caagctatag cgaaatctta
       61 gcaagtaatg aaaatacagc tgaaacgcag ttgcctgaag tcaatttaat caagttagat
      121 aacatcaccg tacaaaactt ataccaagag caacaatctc aacgtttttc aggatttgat
      181 ggctcaatgg gtgtgggtgg ttatgctgga acagttaatg taggcgatat attagaaata
      241 tccatttggg aagcgccacc tgccgtgcta tttggtggaa catttagtgc tgaagggcaa
      301 ggtaatgggc atttaaccca attacctcag caaatggtga acaaaaatgg aacggtaact
      361 gtgcctttta tcggtaatat taaagttgct ggaaaaacac ctgaagtaat tcagaatcaa
      421 attgtcggag cattgagccg taaagctaat caacctcaag cattagttcg tattgcgaat
      481 aacttgtcgt ctgatgttac ggttatccgc caaggaaata gcattcgtat gccattaagt
      541 gccaataatg agcgtatttt agatgcagtt gcggcagtag gtggtacaag cgaaaatatt
      601 gaagatgtaa cagttcgttt aacacgtgga aataaagtgc gttcaatggc gtttgaaaca
      661 ttaattgctg acccctcaca aaatattaca ttacgttcag gtgatgttat tgctttaatg
      721 aatactcctt atagctttac cggattagga gcagtaggta ataatcaaca aatgcgtttt
      781 tcaagtaaag gcattaccct tgctgaagct atcggtaaaa tgggaggatt aattgatgag
      841 cgttcagacc ctcgtggtgt gttcgtattc cgccatattc cttttacaca attagattac
      901 gataaccaag ctcaatggcg tgaaaaaggt tatgcagagg gaatggacgt tccaactgtt
      961 tatcaggtga atttattaaa accgcaatca atgtttttat tgcagcgttt ccctattcaa
     1021 gataaagata ttgtatatgt gtcgaatgct ccactgtctg aatttcaaaa attcttgaga
     1081 atgattttct ccattactac accaattaca ggtacagcta atacaattag agcgtactaa
     1141 ttagctcata gataaaatgg taaaaaatca ttaaaaaatg accgcttgta acacttttcg
     1201 ttgaggattt tatgtcaaag aagaaaaaac attttttagc caaccctttg ttgtggctaa
     1261 ctgtaattat tccaactgct tgttctggaa tttattattc cttatttgcc tctgatattt
     1321 atgtttcaga atctagcttt gtggttcgtt cgcccaataa tcaatcgagc ttaacaggcg
     1381 ttggtgcttt attgcaaaac tccggtttcg cccgttctca agatgatacc tattcagttc
     1441 aggaatatat gcgttcccgt accgcattag aacaacttga agcagaaatt ccggtaaagc
     1501 gtttttatag tgagcaaggt gatttactaa gcaaatttaa cggtttagga tttaacgata
     1561 cccaagaagc attttatcgt tattttaaag aacgtctgaa tattgatgtc gattctattt
     1621 caggtattgc aacgctacgc gttcgtgctt ttaatgcaga agaaggacaa aaaattaacg
     1681 agaatttatt acagcaaggg gaaacattaa ttaaccgcct taatgaaaga gcaagacaag
     1741 atacggttga gtttgcagaa gagcgtgtaa aagaggccga aaaagacgtc aataatactg
     1801 cgttagcatt aagccaatat cgaactaaaa ataaaattta tgatttaccg gcacaatcca
     1861 gcgttcagct ctcattaatt tctaccttaa aaagtgaatt aattcgggta gaaacacaat
     1921 tagcacaatt gaagtcaatt acgccggata atcctcaggt agccgcatta caaatgcgtc
     1981 aaaaaagttt gaaaaaagaa attgatgatc agtccaaaca acttgcagga ggtaatactt
     2041 ctgtagcaac gcaaactgct gaatatcaac gtttggttct tgctaacgaa ttagctcagc
     2101 aacaacttgc agcagcaatg acttcattac aaaatacacg aggtgaagct gatcgccaac
     2161 aactttattt agaggtaatt ggtaggccaa gc
//
LOCUS       DANLRK010000246         2135 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.247, whole genome shotgun sequence.
ACCESSION   DANLRK010000246 DANLRK010000000
VERSION     DANLRK010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.247"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            512..991
                     /locus_tag="PXA33_002503"
     CDS             512..991
                     /locus_tag="PXA33_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HDL5121789.1"
                     /translation="MARVTTELNNTQIKNAKAQTKDYKLMDGKGLFLLVKKNGSKLWR
                     FRYKKPITGKESDLGIGSYPETTLAQARTIREEYRALLAENIDPLEHRQRLEAEALAE
                     IENTFYKVCLKWRDKCESRVKNGELQALTLKKNWRILEKYLLPCRLLYSKHHAKTSY"
     gene            960..1724
                     /locus_tag="PXA33_002504"
     CDS             960..1724
                     /locus_tag="PXA33_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005704989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL5121790.1"
                     /translation="MPNITPKLVINALEPAKEKGIGDTLKRAIRLLNEVMNFAVNADV
                     IEFNKCQNVGSNFSVAKSEHNPTIHPKELPQFLAALRDYNLSFQTKMLIKWQLLTMTR
                     SREASETLWSEIDLEAKTWTISAERMKMKRPHVIPLSRQAVEILERMRKITGNSPFVF
                     QSEMKPRQPMNSQTANRAIGLLGYAGTLTAHGMRSIASTYLNEQLVNYDVVEACLAHV
                     IKDQTRKAYNRSDYLDQRVDVMQQWADYVESCSTGI"
     gene            1774..2064
                     /locus_tag="PXA33_002505"
     CDS             1774..2064
                     /locus_tag="PXA33_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121791.1"
                     /translation="MNNLSEFKLRYINNPYSNKDTFFIFDELSILLKSLTSDELKKIG
                     TAKNLADRQLNKQRKRCKRYVSEAYRLVEDNKEPYSKFFNHDGITAYNIKAF"
BASE COUNT          745 a          395 c          402 g          593 t
ORIGIN      
        1 attttcccta aacattttac ccaagcacag aaaaaatatt tttatgctga tgtatgaaaa
       61 atgaaatctt gctgaagggc tgaatagttg ctgataatag aatagaaatg caactaggaa
      121 agacaaacca acctagccgc acaaatgaac attttataaa cgaaaataaa tagcttttat
      181 gtaaatgatc cctaaaatga tccctatttt tgtagagttt attttatctc tttataaatc
      241 aataagttat aagcgagttc agaggaggct gggaccaact taaattttcc actcttattt
      301 cttcattcgc attcttacgc aaaaccattg atgatactgg ctttagagca attttaaatt
      361 atttcttcat tcgtattctt tcgtgttctt gcgtttacat ccccttattt taagggtata
      421 tttgagggta taacgtaaaa gggtatgaaa cccataccct taaatccctt atgattctga
      481 taaagcacaa tgcgatttta ggtaaatttt tatggctaga gttaccacag aattaaataa
      541 cactcaaatc aaaaacgcaa aggctcaaac caaagactac aagctaatgg acggcaaggg
      601 gctgtttttg ctggttaaga aaaacggctc gaagttatgg cggtttaggt ataaaaagcc
      661 gattaccggc aaagagagcg atttaggcat aggcagttac cccgaaacta ccctagccca
      721 agcaagaacg attagagaag aatatagggc attactagcg gaaaatatcg acccactcga
      781 acaccgccaa agattagagg ctgaagcgtt agccgaaata gaaaacacct tttataaagt
      841 ctgcttgaaa tggcgtgata aatgcgaaag ccgtgtaaaa aatggcgaac ttcaagcctt
      901 aacgctgaag aagaattggc gaatccttga aaagtatctt ttgccttgcc gactactcta
      961 ttccaaacat cacgccaaaa ctagttatta atgctttaga gcctgctaaa gaaaaaggca
     1021 taggcgacac ccttaaacga gcaattaggc tacttaatga agtgatgaat tttgcggtaa
     1081 atgcggacgt tatcgagttt aacaaatgcc aaaatgtagg ctcaaatttt agcgtagcga
     1141 aatctgaaca taacccaacg atacacccaa aagagctacc tcaattttta gctgcattgc
     1201 gtgattacaa cctatcattt caaactaaga tgttgattaa atggcaacta ttaacaatga
     1261 cacgctcacg agaggcaagc gaaacgttgt ggagtgaaat tgatttagag gcgaaaacgt
     1321 ggaccatttc agccgaaaga atgaagatga aacgccctca cgttatccct ctttcacggc
     1381 aagcggtcga aattttagaa agaatgcgaa aaatcacagg gaatagccca tttgtttttc
     1441 aaagcgaaat gaaaccacgc caaccgatga acagccaaac cgccaacaga gccataggct
     1501 tattaggcta tgccggcact ctaacggctc acggtatgcg gtctattgcc tcaacctatt
     1561 tgaatgagca gttagtcaat tatgatgtag tagaggcttg tttagctcac gttatcaaag
     1621 accaaacccg aaaggcttat aaccgttctg attatttaga tcagcgtgta gatgtaatgc
     1681 agcaatgggc ggattatgta gaaagctgct caacggggat ttagtaaatt tttggcggaa
     1741 tttgaccgct tgtttaatta gaggtgtaca aatatgaata acctttctga gtttaaatta
     1801 cgatatatca ataaccctta ttctaataaa gatacatttt ttatatttga tgaattaagc
     1861 atactattaa aatcactgac ttccgatgaa ttaaagaaaa taggaacagc taaaaatctg
     1921 gcagatagac aacttaataa gcaaagaaaa agatgtaaaa ggtatgtatc agaggcttat
     1981 cgtcttgttg aagataataa agaaccttac tctaagtttt tcaaccacga tgggataacc
     2041 gcatacaata tcaaggcttt ctaatctatt tagagagcct tttttatatc gtcctgaaac
     2101 ctctttaatg tgtattatga tcacaatcac aaaac
//
LOCUS       DANLRK010000247         2126 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.248, whole genome shotgun sequence.
ACCESSION   DANLRK010000247 DANLRK010000000
VERSION     DANLRK010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.248"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(134..352)
                     /gene="ilvM"
                     /locus_tag="PXA33_002506"
     CDS             complement(134..352)
                     /gene="ilvM"
                     /locus_tag="PXA33_002506"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HDL5121792.1"
                     /translation="MEEYQLTLKANKRPETLERILRVIRHRGFEVKCLNVESTDSAFN
                     LNFTLASKRPLSLLSHQLEKLFDIIEIN"
     gene            complement(362..2014)
                     /locus_tag="PXA33_002507"
     CDS             complement(362..2014)
                     /locus_tag="PXA33_002507"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HDL5121793.1"
                     /translation="MNGARFITESLKAHGVTTLFGYPGGAIMPTYDAIYDSGLDHLLC
                     RNEQGAAMAAIGYARATGKVGVCIATSGPGATNLITGLGDAALDSIPVVAITGQVASH
                     LIGTDAFQEADVLGLSLACTKHSFIVQNIEELPEILALAFQIAQSGRPGPVLVDIPRD
                     VQLAQTSALPIVYPKEPADAQDPNKLVVAKQLLATAKRPVLYVGGGVSMADGVQAVRN
                     FVKIANIPSVSTLKGLGTIDPTDPLYMGMIGMHGTKAANYAVQECDLLIACGARFDDR
                     VTGKLDTFAPHAKVIHIDIDNAEINKLRQAQVALKGNLIEAVNYLAQPLDIEPWREDV
                     RNLKQQFDFQYQENSGDGNIDAVALLHTLSQRKAKNSIVTTDVGQHQMWSAQHLTYHD
                     PKNYITSAGFGSMGFGLPAAIGAIKARAEDQVILITGDGSIMMNIQELGTLKRADLAV
                     KILLLDNQRLGMVRQWQSLFFHGRHSQTILDDNPDFVLLAKAFDIDGERIEKASEVSD
                     AIDRMFNAKGAYLLHVCIPQEDNVWPLVPPGACNADMLDDEV"
BASE COUNT          621 a          456 c          436 g          613 t
ORIGIN      
        1 gaaaataaag agcaataagt gaaaaaaaag agttgaaatt tgaagaaatg aataaaatca
       61 aaaggatagc gaatgataaa ttttaagcat aaaaaagccg tgtatttttt tacacggcga
      121 atgaaaagtc tgattaattg atttcaatga tatcaaacag tttttctaat tgatggctta
      181 aaagagagag tggacgttta cttgctaatg taaaatttag gttaaaggcg ctgtctgttg
      241 attctacatt taagcatttc acctcaaaac cacggtgacg tataacccgt aaaatacgct
      301 ctaaggtttc agggcgtttg ttagctttaa gcgttaattg atattcttcc atcttattct
      361 cctatacctc atcgtctaac atatcggcat tacacgcccc cggcggtacc agtggccaaa
      421 cgttatcttc ctgtggaata caaacgtgaa gtaagtaagc ccctttggca ttgaacattc
      481 tgtctattgc atctgacact tcgctcgctt tctcaattcg ctcaccgtca atatcaaagg
      541 ctttcgccaa caacacaaaa tcagggttat catctaaaat ggtttggctg tgtcgcccgt
      601 ggaagaacag cgattgccat tggcgaacca tacctaaacg ttggttatcc agtaatagga
      661 ttttgaccgc taaatcagct cttttaagcg tgccgagttc ttgaatattc atcataatcg
      721 agccatcacc ggtaattaaa ataacctgat cttccgctcg agctttgatt gccccaatcg
      781 ccgcaggcaa gccaaaaccc atagagccaa aaccggcaga ggtgatatag tttttcggat
      841 cgtgataagt taaatgttgt gccgaccaca tttgatgctg ccctacatcg gtagtgacga
      901 tgctgttttt ggctttccgc tgtgaaagcg tgtgtaataa cgccacggca tcaatattgc
      961 catcaccact attttcttga tattgaaagt caaattgctg ctttaaatta cgcacatctt
     1021 cacgccatgg ttcaatatct aacggttgag ccaagtaatt cacggcttca atcaaattcc
     1081 cttttaaggc aacttgtgct tgacggagct tgttgatttc cgcattgtcg atatcaatgt
     1141 gaatcacttt agcgtgaggg gcaaaagtgt ctaatttgcc ggtaacacgg tcatcaaagc
     1201 gggcaccaca ggcaatgagt aaatcacact cttgtaccgc gtaatttgct gcttttgtgc
     1261 cgtgcatacc gatcattccc atatagagcg gatcggttgg atcgatagtg cctaaccctt
     1321 ttaaggtaga aacagacgga atatttgcaa tttttacaaa atttctgacc gcttgtacac
     1381 catcagccat actaacgcca ccgccgacat aaagtaccgg gcgtttggcg gtggctaata
     1441 attgtttggc aaccactaat ttgttgggat cttgtgcatc ggcaggttct ttaggataaa
     1501 cgataggcag agccgaagtt tgagctagtt gcacatcacg tggaatatcg accagcaccg
     1561 gaccaggtct gccactttgt gcaatttgga atgcgagggc taaaatttca gggagctcct
     1621 caatattctg aacaataaaa ctgtgtttag tgcaagcgag tgacaaacct agtacatcag
     1681 cttcttggaa ggcatcagtg ccgattaaat ggctggcaac ttgtccggta attgctacca
     1741 caggaataga atcaagtgcg gcatcgccta aaccggtgat taaattagtt gccccagggc
     1801 cggaggtcgc aatacaaacc cctactttac ctgttgcacg ggcataaccg atagctgcca
     1861 ttgcagcacc ttgttcattg cggcaaagta aatgatctaa gcctgaatca taaattgcat
     1921 cataggtagg cataatagcc ccgcccggat agccaaaaag tgtcgttacc ccgtgagctt
     1981 ttaaactctc ggtgataaat cttgctccat tcatggatgt tgtgtcctta atatttttat
     2041 tattttaaaa ccaggcaata tagataacat aaattccctg ttttttaaac tttattttca
     2101 gtaaaaataa ataggattcc atcatt
//
LOCUS       DANLRK010000248         2082 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.249, whole genome shotgun sequence.
ACCESSION   DANLRK010000248 DANLRK010000000
VERSION     DANLRK010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.249"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            67..762
                     /locus_tag="PXA33_002508"
     CDS             67..762
                     /locus_tag="PXA33_002508"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HDL5121794.1"
                     /translation="MARNIIGILGGMGPAATADMFQKFIRLTPADCDQAHIPLLISSI
                     PDIPDRTRYILANGENPAPVMEKYIEGLESAGATCIIIACNTAHYWFEHLKAKSQVEM
                     LSMIDCVVDEVLKCGKWRVGLLATDATLAVDLYKKQLEAQGLTFICPTENGQKAVMDS
                     IYLLKSGELEKATELMLQQRDELIRLGAEVIILGCTEVPIILAQEIEREPMRYVDSTL
                     VLVKKAIDWYQTH"
     gene            778..>2082
                     /locus_tag="PXA33_002509"
     CDS             778..>2082
                     /locus_tag="PXA33_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HDL5121795.1"
                     /translation="MIYAEIFVVLAAIYLGLRSGGLGIGLYGGLGLAILTLGFGLPMG
                     SIPVDVMLIIMTVVIGAAVLQAVGGMDLLVRYAEVLMRKNPKYINFIAPMITWLMTIM
                     AGTGFIVFSTLPVIAEVAKESGIRPSRALAGSVVSSQLAIAGSPLSAAMAAMIAVMEN
                     NSVTFFQVIAVCLPVSFIASMVAAFVASKQGCELQNDVVYLERLKAGIVSPSRKLASE
                     STYSKGAKLSIGIFLLATVLVVLLAAVPGLKPVYENGKSMSTRDIIIVMMLASSCLMM
                     LVGKIKPDTIVASPIFRSGMTSIAVIIGIVTLGMTFVDAHLIEIKASIGNILTEYPML
                     LAVVLFFTCALLYSQGSTSALIIPLAVSLGIPNWAILASFIAITGIFILPTYPTSLAA
                     MELDTTGSTRAGKYIIDHPFMLPALVGVAVGLAFGFVWAPMIV"
BASE COUNT          580 a          333 c          467 g          702 t
ORIGIN      
        1 tttctttttt gcataacttg tttgtataag tcatcgcagt aaattaaatc ttataaggag
       61 aagtgtatgg caagaaatat tattggcatt ttaggtggaa tgggacctgc agcaacagca
      121 gatatgttcc aaaaatttat ccgcttaaca ccagctgatt gtgatcaagc tcatattcca
      181 ttattgattt cctctattcc cgacatacca gatagaactc gctatatttt agcaaatgga
      241 gagaatccgg ctccggtaat ggaaaaatat attgaagggc ttgagagtgc aggggcaacc
      301 tgcatcatta ttgcatgtaa tactgcacat tattggtttg aacacttgaa ggccaaaagt
      361 caggtagaga tgctaagtat gattgattgt gtggttgatg aggttttgaa gtgtggaaaa
      421 tggcgggttg gattgttggc aactgatgca actttggcgg tagacctata caaaaaacag
      481 cttgaagcac aaggactgac ttttatttgc ccgacagaaa atggtcaaaa ggcagtaatg
      541 gatagtatct atttattaaa atcaggtgag ttagagaaag ccacagagct aatgttacaa
      601 caacgtgatg aactcattcg tttaggtgca gaagtaatta ttttaggttg tacagaagta
      661 cctattattt tagctcaaga aatagagcgt gagccaatgc gttatgtaga ttcaacgttg
      721 gtattggtta aaaaagcaat tgattggtat caaactcatt aatttaagga gatggatatg
      781 atttatgcag aaatctttgt ggtacttgct gcaatctatt taggactgcg ttctgggggc
      841 ttaggaatag gactatatgg tggtttaggg cttgcgattc taactctagg ttttggttta
      901 cctatgggtt ctattccagt tgatgtaatg ctgattatta tgactgttgt aataggtgct
      961 gctgtgttac aagcagtagg aggtatggat ttacttgttc gttatgcgga agtattgatg
     1021 cgtaaaaatc ctaaatatat taactttatt gcaccgatga ttacttggtt aatgacgatt
     1081 atggctggta caggttttat cgtcttttcc accttacctg tgattgcaga agtggcaaaa
     1141 gagagtggta ttcgcccttc aagagcatta gccggctcgg ttgtgtcttc tcaattagct
     1201 attgcaggtt ctccattgag tgctgcaatg gcggctatga tagcagtaat ggaaaacaac
     1261 agtgttacat tttttcaagt aatagcagta tgcttacctg tttcttttat tgcctctatg
     1321 gttgctgcat ttgttgcttc taagcagggc tgtgaattgc aaaatgatgt tgtttattta
     1381 gaacgtttaa aagcaggaat cgtcagccca tctcgtaagt tagcgagtga atctacctat
     1441 tcaaaaggag caaaactatc gattggcatt ttcttactag caactgtatt ggttgttcta
     1501 cttgcagccg ttccagggtt aaagccagta tatgaaaatg ggaagtcaat gagtacccga
     1561 gatataatta ttgtgatgat gttagcctct tcgtgcttaa tgatgttggt aggaaaaatt
     1621 aaacctgata cgattgtggc tagtccaatt ttccgctcag gaatgacttc tattgcggta
     1681 attatcggga ttgttacatt aggtatgact tttgtcgatg cacatttaat agaaattaaa
     1741 gcctctattg gaaatatttt aactgaatat ccaatgttgt tagctgtagt actctttttt
     1801 acttgtgcat tactttattc acaagggtca acttctgctc ttattattcc attagcagta
     1861 agcttgggga tccctaactg ggcaattttg gcttctttca ttgcaattac aggcattttt
     1921 atcctgccaa cctacccaac ttcattagca gcgatggaat tagatactac gggttcaact
     1981 cgtgctggca aatatattat agatcatccg tttatgcttc cagctttagt tggggttgct
     2041 gttgggcttg cttttggatt cgtatgggct ccaatgattg ta
//
LOCUS       DANLRK010000249         2077 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.250, whole genome shotgun sequence.
ACCESSION   DANLRK010000249 DANLRK010000000
VERSION     DANLRK010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.250"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            166..606
                     /locus_tag="PXA33_002510"
     CDS             166..606
                     /locus_tag="PXA33_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3577 domain-containing protein"
                     /protein_id="HDL5121796.1"
                     /translation="MTTQTSTQKNYFNLHTTGIGYLSNIRTVTPKKGDEFLACTIGAL
                     IGEAGEGAKAEYRYFDCKVVGEAAIDLVNRAKASVEADKKVLISFVLADLWTDTFTYQ
                     KDGKNHKKGDTGVMLKARLIRIKSVKIDGVVKYQEPEKIDAQSE"
     gene            713..1360
                     /locus_tag="PXA33_002511"
     CDS             713..1360
                     /locus_tag="PXA33_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HDL5121797.1"
                     /translation="MTINTYAKFAPTVFVAKCPEAHTKGEIITLTSKYGNETEVQIHN
                     LVKQDNEHYYYSFTRCDGMDSQSFAQQKAERYQGYADNATKRSQSWANAANEGLEFLR
                     LGEPIKIGHHSEKRHRNLIERNARRMDNALSEMNKTESYESRIAYWEKMAGKIDLSMP
                     ESLEYFKFELDKAKEKHKELKDNPEKREHGYSLTYAKKAVNELEKKVKLAELLWA"
     gene            1384..1791
                     /locus_tag="PXA33_002512"
     CDS             1384..1791
                     /locus_tag="PXA33_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme utilization protein"
                     /protein_id="HDL5121798.1"
                     /translation="MNRQGVYINGKQVFDSKGKEINSIEIIGSTVQTVVGINKGSISM
                     VSSNNALIVDGQTIAFEPSPTIHIEVFGNITNLNSGAGNVNIKGDVSHIETVSGDVNV
                     DGTVTSDVRTVSGDIRCKTIAGKVETISGDIYR"
     gene            1869..>2077
                     /locus_tag="PXA33_002513"
     CDS             1869..>2077
                     /locus_tag="PXA33_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121799.1"
                     /translation="MKDILLDFDDTSCYLGDWEEILEEYNDRVRVEDFWHYAKEFETV
                     PHLGNLYQEHVILKLIARFCIELDI"
BASE COUNT          686 a          360 c          437 g          594 t
ORIGIN      
        1 agcattccga aagggaactc cacaatattg tgggaatgcc gattaaacct ctaccatttc
       61 ggtgaattta catcgaaatt tcatttaact tccaccttgc ggggaacatt tttcccgtgt
      121 tgggtaagga tgtttctccg cttttttact ttggagaaac agactatgac aactcagact
      181 tctactcaaa aaaattactt caatcttcac acaaccggaa tcggctatct gagcaatatc
      241 cgcacagtga ctccgaaaaa aggtgatgaa ttcttagctt gtactattgg tgctctcatt
      301 ggagaagcag gagaaggtgc aaaagcagaa tatcgctatt ttgattgcaa agttgtgggc
      361 gaagctgcga ttgatttggt caatcgggca aaagcctctg ttgaagctga taagaaagta
      421 ctcatctctt tcgttttagc tgacctttgg acagatactt tcacctatca aaaagatgga
      481 aagaatcaca aaaaaggtga tactggtgta atgctgaaag ctcgcttaat tcggattaag
      541 tctgtcaaaa ttgatggagt ggtaaaatac caagaaccag aaaaaataga cgcacaatct
      601 gagtaatttt acctaagccc gacattttat gttgggcttt actttatgtc ccgtagggaa
      661 atgctccctg cggtgtgttt cccttcattt ttacaatttt aggagaaaca ctatgactat
      721 taacacttat gcaaaatttg cacctactgt tttcgtagca aaatgccctg aagcacatac
      781 caaaggtgaa attatcactc taaccagcaa atatggtaat gaaaccgaag ttcaaataca
      841 taacttagtg aagcaggata atgagcatta ttactattct ttcactcgtt gtgatgggat
      901 ggatagtcag tcatttgctc aacaaaaagc agaacgctat caaggctatg cagataatgc
      961 aaccaaacgc agccaaagct gggcaaatgc agctaatgag gggctggagt ttttgagact
     1021 aggtgagcca ataaaaattg ggcatcatag tgaaaaacgc caccgtaacc tcattgaacg
     1081 taatgcccgt cgaatggata atgcgctttc agaaatgaac aaaacagaaa gctatgaatc
     1141 ccgtattgca tattgggaaa aaatggcagg gaaaattgac ctttctatgc cggaaagtct
     1201 ggaatacttt aagtttgaat tggataaagc caaagaaaag cataaagaat tgaaggacaa
     1261 ccctgagaaa cgagagcatg gttattcact gacttatgct aaaaaagcag ttaatgaact
     1321 tgagaaaaaa gtgaaattag ctgaattact ttgggcataa tttttacata gaggattatt
     1381 aatatgaatc gacaaggcgt ttatatcaat ggtaaacaag tctttgactc taaaggcaaa
     1441 gagattaata gcattgaaat cattggtagc acagtgcaaa cagtcgttgg gataaataaa
     1501 ggatctatct caatggtctc cagcaacaat gcattaattg ttgatgggca aacaattgca
     1561 tttgaacctt ctccgactat ccatattgaa gtatttggta atattaccaa tctcaattca
     1621 ggagcaggaa atgtgaatat caaaggtgat gtaagccata tagagactgt gagtggtgat
     1681 gtgaatgttg atggtactgt gacttcagat gtaagaacag taagcggtga tattcgctgt
     1741 aaaactattg caggtaaagt cgaaaccatc agcggagata tttatcgcta atttttgcta
     1801 cttaccctat cgggaaacat tcccgatagg tgtgtttccc cacttttatt taaattttgg
     1861 agaaacatat gaaagacatt cttttggatt ttgatgacac ctcttgttat ttaggtgatt
     1921 gggaagagat actagaagaa tacaatgatc gtgttcgagt tgaggatttt tggcattacg
     1981 ctaaagaatt tgaaaccgta cctcatttgg gcaacctata ccaggagcat gtcatcctga
     2041 agttgattgc tcgattctgt attgaactgg atattga
//
LOCUS       DANLRK010000250         2059 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.251, whole genome shotgun sequence.
ACCESSION   DANLRK010000250 DANLRK010000000
VERSION     DANLRK010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.251"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            <1..452
                     /locus_tag="PXA33_002514"
     CDS             <1..452
                     /locus_tag="PXA33_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HDL5121800.1"
                     /translation="IYLGLIVVQGIYAYKFIKSLYHLVINLNEMDSNTVMLAVLNLID
                     VVMIANLLVMVIVGGYETFVSRLKVDDHPDQPEWLDHVNATILKVKLSMAIISISSIH
                     LLQTFINASNLDEKQMMWQVVIHLAFIVSALAMAYIDKMLNSGHMKH"
     gene            529..1899
                     /gene="gorA"
                     /locus_tag="PXA33_002515"
     CDS             529..1899
                     /gene="gorA"
                     /locus_tag="PXA33_002515"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009499791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HDL5121801.1"
                     /translation="MAKHYDYIAIGGGSGGIASINRAASYGQKCAIIEAKHLGGTCVN
                     VGCVPKKVMFYGAQVAEAINHYAPDYGFDITVNNFDYAKLVESRQAYIGRIHTSYGNV
                     LAKNNVDVLNGFARFKDAKTLEVSYADGSVEEVTADHILIATGGRPTVPNVKGAEYGI
                     TSDGVFALDALPKSVAIVGAGYIAVELAGVFNSLGVNTHLFVRQHAPMRNQDPLIVDT
                     LLEVLAQDGIELHTQATPQEVVKNADGSLTLKLQDGRETSVESLVWAIGREPATDKIN
                     LEAAGVETNERGFIKVDKFQNTNVPNIYAVGDIIEGGIELTPVAVAAGRRLSERLFNN
                     KPNEHLDYSLVPTVVFSHPPIGTVGLSEPKAIEQYGAENVKVYKSSFTAMYTAVTQHR
                     QPCRMKLVCVGEGEKVVGLHGIGFGVDEMIQGFAVAIKMGATKADFDNTVAIHPTGSE
                     EFVTMR"
BASE COUNT          623 a          295 c          456 g          685 t
ORIGIN      
        1 ctatttattt agggttgatt gttgtacagg ggatatatgc gtataagttt attaaatcat
       61 tgtatcatct agttatcaat ttaaatgaaa tggattcaaa tactgttatg ttagcggtat
      121 taaatttaat agatgtggtg atgattgcaa atcttttggt tatggttatt gttggaggat
      181 atgaaacttt tgtctctaga ttaaaagtag atgatcaccc tgatcagcca gaatggttgg
      241 atcacgttaa tgcgactatt ttgaaagtaa aactttcaat ggcaattatc agtatttctt
      301 ctattcattt attacaaaca tttatcaatg cttcaaatct tgatgaaaag caaatgatgt
      361 ggcaagtagt tattcactta gcctttattg tttcagcatt agcgatggcg tatattgata
      421 aaatgttgaa ttcagggcat atgaaacatt agtcttctat gcaaatttaa tttgataaag
      481 cgcttaacga ataagttgct tttgttttta tagatcgagg aatagcttat ggctaaacat
      541 tatgattata ttgctatcgg cggtggtagt ggtggtattg cttcaattaa tcgagcagca
      601 agctatgggc aaaaatgtgc cattattgaa gcaaaacatt taggtggcac ttgtgtgaac
      661 gtgggatgtg tacccaaaaa agtgatgttc tatggtgcac aagttgcaga agcgattaac
      721 cattacgccc ctgattatgg ttttgacatt acggtaaata attttgatta tgccaaatta
      781 gttgagagtc gccaggccta tattgggcgc attcatacct catatggtaa tgtgctggca
      841 aaaaataatg ttgatgtttt aaatggtttt gctcgcttta aagatgctaa aacattagaa
      901 gtgagttatg ctgatggttc cgttgaggag gtcacagcag accatattct gattgcaaca
      961 ggtggtcgtc caactgtacc caatgtgaaa ggggctgaat acggtatcac ttctgatggt
     1021 gtatttgcac tagatgcttt accgaagtcc gtagcaattg tgggggcagg gtatattgca
     1081 gttgaattag ctggagtatt taatagttta ggagtaaata cccatctttt tgttcgtcaa
     1141 catgctccaa tgcgtaatca agacccatta atagtggata cattgcttga agtattagct
     1201 caagatggga tcgaattaca tacacaagcg actcctcaag aagtagttaa aaatgcagat
     1261 ggttcattga cattaaaatt gcaagatggt cgtgaaacct ctgttgaaag cttggtgtgg
     1321 gctattggtc gtgagccagc gacagataag attaatttag aggctgctgg tgttgaaacc
     1381 aacgagcgtg gttttatcaa agtggataaa ttccaaaata ctaatgtgcc aaatatctat
     1441 gctgttggtg atattattga aggtggtatt gaattaactc ctgttgcagt tgcagctggt
     1501 cgcaggctat cggaacggtt gttcaataat aaaccaaatg aacatttgga ttatagctta
     1561 gtaccgacag tcgtttttag ccacccacca ataggtactg ttggtttaag tgagccaaaa
     1621 gcgattgagc aatatggggc agaaaatgtg aaagtgtata aatcttcatt tactgcaatg
     1681 tatactgcgg ttacacaaca tcgtcaacct tgccgaatga aattagtttg tgttggggaa
     1741 ggggaaaaag tggttgggtt acatggtatt ggttttggtg ttgatgaaat gattcaaggt
     1801 tttgctgttg caattaaaat gggcgcaacg aaagctgatt ttgacaacac agtggctatt
     1861 cacccgacag gatctgaaga atttgtaacc atgcgctaaa tgtaataaaa tgttacgtaa
     1921 ttttatgatt atgctacatt ttatttatgg gtttgctatg aaaaacgctt ttagcttttt
     1981 tgttatttct ctttttctta cgatgaatag tattagttat gcaagtaatc ctattcctag
     2041 taataaagaa aaattaaac
//
LOCUS       DANLRK010000251         2048 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.252, whole genome shotgun sequence.
ACCESSION   DANLRK010000251 DANLRK010000000
VERSION     DANLRK010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.252"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            6..353
                     /locus_tag="PXA33_002516"
     CDS             6..353
                     /locus_tag="PXA33_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121802.1"
                     /translation="MFREEAIEYQSKKWKSTAVLFSRVPALVVFSISFFIFTAFILFI
                     VFGNYTRRETVVGELVMQSHPILLSAPKSGYISENYIQPHQQVKKRAIRWLKLRWIEL
                     RIAKILMLILFFR"
     gene            350..1267
                     /locus_tag="PXA33_002517"
     CDS             350..1267
                     /locus_tag="PXA33_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HDL5121803.1"
                     /translation="MKSQIQATEQAITALYKNKSETINSLEKQIKNSQKIYQDKQSYL
                     VEIEKSMNDYAELVKRYEKLLKVGHSSHDEVNIQKSRYFQQKSLFNELKQELIQLKST
                     QLSLENEIETRKTEFNNQIIRYEMQKSDLNIRLMEFESVSDIIVNASLDGKIESTSVT
                     VGQIIKENDPLAQILPQNKGNYQLVMWVPNSAISFIKQGDEVNIRYEAFPFEKFGQFK
                     GKITSISTLPASLQELSFYKNLPVNLEQGIPLYKILIELADQNVKYNDTSLYFMSGMK
                     AEATLFLEKRKLYEWIFFPMYQLRKNMEQ"
     gene            1267..>2048
                     /locus_tag="PXA33_002518"
     CDS             1267..>2048
                     /locus_tag="PXA33_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HDL5121804.1"
                     /translation="MTGIKQLKFDFRRRIPVILQTETAECGLACLAMMLGYYDKNTNL
                     FELRSQYGTSSRGVTLHTLISIANDCGLITRPLSLELDEVPQLRLPCILHWDFNHFVV
                     LTHASEKQFIIHDPAFGKRKLSRAEFSNHFTGVALEVWSEVKFEKSNNENTISLYETL
                     KHISGIKGALVKIFALSMLIELIALLMPIGTQLVMDHVMQAKDQSLLLIICIGLFLFT
                     LFRTMVSMFRAWVSLKMNYLIDFQWTASFFSHLLKLPLDFFE"
BASE COUNT          684 a          335 c          359 g          670 t
ORIGIN      
        1 attatatgtt cagagaagaa gctattgaat atcaatcgaa aaaatggaaa agcacagctg
       61 tactatttag cagagttcca gctttggttg tcttttccat ttctttcttt atctttactg
      121 cctttattct atttattgtg tttggtaatt atacccgccg tgaaacggtt gtgggtgaat
      181 tagtgatgca atctcatccg attttattat ctgccccaaa atccggctat atatccgaaa
      241 attatattca accacatcag caggtaaaaa aaagggcgat ccgctggtta aaattacgct
      301 ggatagaatt acgcatagcg aaaatattaa tgttaattct attctttcgt tgaaatcaca
      361 aattcaagca acagaacaag cgataactgc tttatataag aataaatcag aaactattaa
      421 tagccttgaa aagcagatca aaaacagtca aaaaatttat caagataagc agagttattt
      481 agtggaaatt gaaaaaagta tgaatgacta tgcggagttg gttaaacgct atgagaaatt
      541 attaaaagta gggcattcca gccacgatga agtgaatatt cagaaaagcc gttatttcca
      601 acaaaaatca ctgtttaatg agctaaagca agaattaatt caattaaaat ccacccaact
      661 tagcttggaa aatgaaattg aaacccgtaa aaccgaattt aataatcaga ttattcgcta
      721 tgaaatgcaa aaaagcgatt taaacattcg tttaatggaa tttgaatctg tttcagatat
      781 tattgttaat gcctctttag atggaaaaat tgaaagtacc agcgtaacag ttgggcaaat
      841 tattaaagaa aatgacccgc ttgcacaaat tctgccacag aataaaggaa attatcagct
      901 agtgatgtgg gtaccgaata gtgccatttc atttattaaa caaggcgatg aggtgaatat
      961 tcgctatgag gcttttccgt ttgaaaaatt cgggcagttt aagggcaaaa ttacgtcaat
     1021 ttcaacctta ccagcttctt tgcaagaact tagtttttat aaaaatctcc ctgtaaattt
     1081 agagcaaggt attccgcttt ataaaattct gattgagctt gccgatcaaa acgtaaaata
     1141 taatgatacc tccctttatt ttatgagtgg aatgaaagcc gaagccacgt tatttttaga
     1201 aaaacgtaaa ctgtatgagt ggatattttt ccctatgtat caattacgca aaaatatgga
     1261 gcaataatga caggaattaa gcagttaaaa tttgattttc ggagaagaat acctgttatt
     1321 ttgcaaactg aaacagctga atgtggctta gcctgtttag cgatgatgtt gggctattac
     1381 gataaaaaca ccaatttatt tgaattgcga agtcaatatg gtacttcctc tcgtggcgta
     1441 acgctgcata ctttaatttc tattgcaaat gactgtggtt taataactcg ccctctttct
     1501 ctagagttag atgaagtacc acaacttcgt ctgccttgta ttcttcattg ggattttaat
     1561 cattttgtag tattaactca cgcaagtgaa aaacagttta ttatccacga tcctgctttc
     1621 ggtaaacgta aattatcccg tgctgaattt tccaaccatt ttaccggagt cgctttagaa
     1681 gtttggtctg aagttaaatt tgagaaaagc aacaatgaaa ataccattag tttgtatgaa
     1741 accttaaagc atatttctgg cataaaaggt gcattagtga aaatttttgc cctttcaatg
     1801 ctcattgaac tcattgcctt attgatgcca attggaacac agttagtgat ggatcacgtt
     1861 atgcaagcga aagatcaatc attactttta attatctgta ttgggctatt tttatttacc
     1921 ctttttcgca caatggtcag tatgtttagg gcttgggttt ccttgaaaat gaattattta
     1981 attgattttc aatggacagc cagtttcttt tctcatctct taaaattacc gcttgatttc
     2041 tttgaaaa
//
LOCUS       DANLRK010000252         2038 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.253, whole genome shotgun sequence.
ACCESSION   DANLRK010000252 DANLRK010000000
VERSION     DANLRK010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.253"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            <1..253
                     /locus_tag="PXA33_002519"
     CDS             <1..253
                     /locus_tag="PXA33_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121805.1"
                     /translation="IALWTVYSHLDTKVEARFSKIDDKFVQVETNIKNLDIRLTKVES
                     RLDNVEQRLDNVEQRLGNMEKKIDGVDNKLDLLLQQFKR"
     gene            385..567
                     /locus_tag="PXA33_002520"
     CDS             385..567
                     /locus_tag="PXA33_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HDL5121806.1"
                     /translation="MKYSEFLRYLLAQGCEIENHRRGSHRKVTLNGKQSVFPYHGSKE
                     IGTGLVNKIKKDLDLK"
     gene            606..1025
                     /locus_tag="PXA33_002521"
     CDS             606..1025
                     /locus_tag="PXA33_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012621799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HDL5121807.1"
                     /translation="MLRYPVEITPDDNGTYLVTCPDIPEMASVGEDLEEALLEAQDGL
                     ATALEFYFDDRREIPMPSPIKEGQHTVNLTVLQSMKVFLLNEMIKQGVRKAEMARRLD
                     VHLPQIDRLLDFNHSTKVEFVEKAYGKLNQHFTILPH"
     gene            1361..2005
                     /locus_tag="PXA33_002522"
     CDS             1361..2005
                     /locus_tag="PXA33_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL5121808.1"
                     /translation="MFGHIWQYAREWGYTTLASPNTGMKRFPIRKREIYVENNIFEMV
                     YEVADQDIKDLMDLAYLTGQRPVDLVNIQRDHIFDGYLHIVQQKTQAKLRIEIVGKLA
                     DIIQSRLEKTTRNFLFTTSKGEKMTSVKLGRRFTYIREQAAKKHPEYANEIKEFQFRD
                     LRAKSGTDKALAQGEEAARQQLGHTSVQMTKVYIRKAPIVTPIQESVSSQKHKP"
BASE COUNT          766 a          385 c          349 g          538 t
ORIGIN      
        1 aattgcatta tggacagtat attcacacct tgacaccaaa gttgaagctc gattctcgaa
       61 aattgatgat aagtttgtcc aagtagagac caatatcaaa aatcttgata ttcgcctcac
      121 taaagttgaa tctagattag ataatgtcga acaacgacta gacaatgttg agcagcgact
      181 aggcaatatg gagaagaaaa tagatggggt tgataataaa ctcgatctgt tactacaaca
      241 attcaaaaga taaaataata ttccttgaat aaaccgccct cgtggcggtt ttcttttctg
      301 ctacaaaaaa aacacaacaa aaatgttgta ataccaacaa aaatgttgta ttatttcctt
      361 gctgttttga taaggaggta tcttatgaaa tacagcgagt tcctgaggta cttacttgct
      421 caaggttgcg aaattgaaaa ccataggcga ggcagtcatc gaaaggtaac actaaacgga
      481 aaacaatcgg tttttcctta tcacgggagc aaagagattg gtacaggttt agtaaataag
      541 attaagaaag acttagatct taaataacaa agtccctcga aagaggggct tttctaggag
      601 tttatatgtt acgctaccca gttgaaatta cccctgatga taacggcaca tatcttgtga
      661 cttgccctga tattcctgaa atggcaagcg taggtgagga cttagaagaa gccctattag
      721 aagcacaaga tggcttagcc acagcccttg aattctattt tgatgataga cgagaaatac
      781 caatgccaag cccaattaaa gagggacaac atacggttaa tcttaccgtg ctgcaatcaa
      841 tgaaagtatt tctcttaaat gaaatgatta aacaaggtgt acgcaaagca gaaatggcaa
      901 gacgattaga tgtacactta ccacagatag atcgcctatt agattttaat cattccacaa
      961 aagtagaatt tgttgagaaa gcctatggta agctaaatca acactttact attctccctc
     1021 actaataaaa taaccgccta cgggcggttt tctttttcct aaaatctgca aaattctctt
     1081 caaaaacgac cgcttgcaac tcaatctgtt tatttattaa gcaatcaaac aacctttcta
     1141 aaaataaatt ctctttaaaa tcaataaatt actaaacaga actaaacttt atttattttc
     1201 cactaaataa acactttact aaatgtttaa tatttactaa actacaccca tcaaaacaaa
     1261 caacgccaca gacaaagggg aaaccaaaat gaacgcacaa acaacgctaa acaaccacaa
     1321 agactacatt cactaagaat ttttatcaaa gcccttttca atgttcggtc atatttggca
     1381 atacgcaaga gagtggggtt acacaacgtt agctagtcct aatacaggaa tgaaacgctt
     1441 tccaattaga aaacgtgaaa tttacgttga gaacaatatt tttgaaatgg tgtatgaagt
     1501 agcagatcag gatattaaag atttaatgga tctcgcttac cttacaggac aaagacctgt
     1561 agatttagtt aatatccaac gcgatcatat atttgatgga tatctccata ttgtgcaaca
     1621 aaaaacacaa gccaaactgc ggattgagat agttggcaaa cttgctgata taattcaaag
     1681 taggttagaa aaaaccacaa ggaatttctt atttacaacg tcaaaaggag aaaaaatgac
     1741 ctctgtaaaa ttaggaagac gctttactta tatccgagaa caagcagcaa aaaaacatcc
     1801 cgaatacgca aatgaaataa aggaattcca atttagagat ctaagggcta agtcagggac
     1861 agacaaagcc cttgcacaag gagaagaagc tgcacgccaa caattaggtc atactagcgt
     1921 tcaaatgaca aaagtctata tccgaaaagc cccgatagta accccaattc aagaaagcgt
     1981 ttcctcccaa aaacacaaac cttaaaacct agtttcggaa catctgagaa tttttcgg
//
LOCUS       DANLRK010000253         2001 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.254, whole genome shotgun sequence.
ACCESSION   DANLRK010000253 DANLRK010000000
VERSION     DANLRK010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.254"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            1..1111
                     /locus_tag="PXA33_002523"
                     /pseudo
     CDS             1..1111
                     /locus_tag="PXA33_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253679.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
     gene            1139..1873
                     /locus_tag="PXA33_002524"
     CDS             1139..1873
                     /locus_tag="PXA33_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDL5121809.1"
                     /translation="MARSSYFARLAEWTLLEHLLVKQPENISVCSFGQSFGTSGRLDA
                     EYYQPKFASLFAQLANFKTMKLGEIVNLQKSVEPGSEAYQTEGIPFVRVSDLSKFGIS
                     QTDKYLHPKDFGNVVRPKKDSILLTKDGTVGIAYRVPQDLNVITSGAIVHLELKTDEV
                     LPDYLALALNSPAVQLQAERDAGGSIIQHWKPSEILDVVIPVLPKNIQQTISDKVQQS
                     FALRVESEVLLEKAKILVEQEIENMR"
BASE COUNT          714 a          304 c          380 g          603 t
ORIGIN      
        1 gaaagaaggg gatgttttag taaatatcgt tggtgcaact ttggaggttg ttggtcgggt
       61 agcttatgtt agtagcactt ttccaaaagc aaatattaca caagcaatgt cttttgtaag
      121 attaaaatca aaatataata aagaattatt accaacgtat ttatttgctt ttttacaaag
      181 ttcttatgga aaaatccaaa ttaaccggaa tgcacgccca acaggacaat acaatctgaa
      241 taatgaggag cttggtgcga ttaaagtccc cttaattgat ctagaaacac aaaagcaggt
      301 agataaaatc attaagcaaa gtaatgattt tgtacaaaaa tcaacacaaa gttaccaaga
      361 agctgaaact ttattattag aaaatctcgg tttgagagct tttcaagctg atagcaatcc
      421 tgtaaatgtt aaatccttaa aagagagttt cttacaaaca ggacgattag atgcagagta
      481 ttatcaaact aaatatgagc aatactggaa tttaattcaa tcgcaagatt atgtttttat
      541 tcgtgatgaa tatttacata tcacccaaaa acctgattgg acaaaaccga tgtatcaata
      601 tattgaaatt ggtgatgtta atattggtga tggttcatat caaacgaatt ggatagagac
      661 acaagaatta cctgctaatg ctaaaactca agcccaaaca ggtgatattt taatttcaac
      721 cgttcgccct tatcgtggtg cagtaacaat tatcggtgaa aatgatcaag atttagttgt
      781 tagtggtgcg tttacagtat tacgcagaaa ggaaaactct gtttttaata atgaagtttt
      841 aaaggtgcta ttacggtcag aactatataa agattggtta ctacaattta atgtaggtac
      901 gagttatcct gtaattaaag ataacgatat tttgaattta ccaattccta aaataagtgg
      961 agaaatccaa gaaaaaattg ctgaatatat ccgccaaagc aacgatttaa ggcagcaagc
     1021 acaaaattta ctcgctcaag ctaaaaataa tgtagagttt gaaattgaaa ataattcatt
     1081 taatatcaat gagttagaag tgcggggggg ggtagatcct gctaagcctt atggtttatt
     1141 ggcgagatct tcttattttg cacgtttagc agaatggact ttattagaac atttacttgt
     1201 caaacagcct gaaaatattt cggtatgttc attcggtcaa tcattcggta caagcggtcg
     1261 tttagatgca gaatattacc aacctaaatt tgcaagctta tttgcacaat tagccaattt
     1321 caaaacgatg aaattaggtg agattgttaa tttacaaaaa tcggtagaac cgggctcgga
     1381 agcttatcaa acagagggaa taccctttgt gcgtgtatcg gatttatcta aattcggcat
     1441 cagccaaaca gataaatact tacatccgaa agattttggc aatgttgtaa gacctaaaaa
     1501 agacagtata ttactgacta aagacggcac agtcggcatt gcttaccgtg ttccgcaaga
     1561 tttgaatgtg attacatcag gggcaattgt gcatctagag cttaaaactg acgaagtttt
     1621 acccgattat ttggcgttag cattgaattc tcctgctgta caattacaag cggaaagaga
     1681 tgcgggaggc tctattatcc aacattggaa accgtctgaa attttagatg tagtgattcc
     1741 ggtattacct aaaaatatcc agcaaaccat tagcgacaaa gtacaacaaa gttttgcatt
     1801 gagagtagaa agtgaagttt tactggaaaa agctaaaata cttgtagaac aagaaataga
     1861 aaatatgagg taataatgcc taatcataat caagatttga aaaaagagtg gtttaataaa
     1921 gcaaggatag attaccactc tcctttttta atgttatggc tcagttgtaa tagctggtat
     1981 aacttccatt attccttagg t
//
LOCUS       DANLRK010000254         2000 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.255, whole genome shotgun sequence.
ACCESSION   DANLRK010000254 DANLRK010000000
VERSION     DANLRK010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.255"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(<1..1169)
                     /locus_tag="PXA33_002525"
     CDS             complement(<1..1169)
                     /locus_tag="PXA33_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HDL5121810.1"
                     /translation="MSIQQKKRHSRWYERIFFSTAVQSILGVMFSSVLPAIYFWGEKF
                     NSIETRSNTIIATSLAFVMIAISLRVLMKYPGNKSASFVFSTVISWYAVLLIVLTIFR
                     FDYSNTLLGTSFILTILFCFIGYFLSRSWIIPKIALVPFGRAINLNRIQNVEWIQLSE
                     PILGEKRINSIAVDLHYPDLSAEWHKFLAKCTLNGIPVYNVRQLEESLTGRVKIRHMY
                     ENDLGSLLPSPIYSLVKRIIDIALIVATFPITLPIMTITAIAIRLESEGGAMFIQKRV
                     GQGGKEFNIYKFRSMCKDSEKDDAQFASSADMRVTRIGKFIRKTRIDELPQFFNVLKG
                     DMSLIGPRPEQTAFVDKFEEEIPFYNYRHIVKPGISGWAQVVQGYAADVDDTKVKV"
     gene            complement(1173..1981)
                     /locus_tag="PXA33_002526"
                     /pseudo
     CDS             complement(1173..1981)
                     /locus_tag="PXA33_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_162680708.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
BASE COUNT          687 a          405 c          293 g          615 t
ORIGIN      
        1 acttttactt ttgtatcatc aacatctgcc gcataacctt ggacgacctg agcccaaccg
       61 gaaatacccg gtttaacgat atggcgataa ttatagaatg gaatttcctc ttcaaattta
      121 tctacaaagg ctgtctgttc tgggcgagga ccaattaagc tcatatcacc ttttaataca
      181 ttaaaaaact gaggtagctc atcaatacga gttttacgga taaatttccc aatacgagtt
      241 accctcatat ccgcactcga agcaaactgt gcatcatctt tttcggagtc tttacacata
      301 ctacgaaatt tataaatatt aaactccttt ccaccttgcc caacgcgttt ttgaataaac
      361 attgctccac cttcactttc taagcgaata gcaatagcgg tgattgtcat aataggtaaa
      421 gtaatgggaa acgtagcaac aatcaatgca atatcaatga tacgcttcac taatgagtaa
      481 attggagaag gaagtaatga acctaagtca ttttcataca tatggcgaat tttgacacgc
      541 ccggttaaag attcctcaag ctggcgaacg ttatatacag gaataccatt caatgtacat
      601 ttcgctaaaa atttatgcca ttctgcggac aaatcaggat agtgtaaatc taccgcaata
      661 gagttaattc tcttttcccc caatattggt tcacttaatt gaatccactc tacattctga
      721 attcggttca aattgatagc tcttccgaat ggaaccaaag cgattttagg gataatccaa
      781 cttcttgaaa gaaaataacc aataaaacaa aatagaattg tgagtataaa gctcgtacct
      841 agcaatgtat tagaataatc aaatctaaat attgttaaca cgattaataa aaccgcatac
      901 caagaaataa cggtggaaaa aacaaaactt gctgacttat tacctggata tttcattaac
      961 actcttaagc tgatagctat cattacaaag gcaagagaag tcgctataat cgtattagaa
     1021 cgagtttcaa tagaattaaa tttctctccc caaaaataga tagcgggtaa gacacttgag
     1081 aacataacgc ccaaaatact ttgaacagct gtactaaaaa agatacgttc ataccaacga
     1141 gagtgtcttt tcttttgctg aatgctcata atttatcctt ttcttaattt cttataaagt
     1201 cttcctaaaa gagatctagg caataaccgt ggtaaggctc taagtataaa aaacatgctt
     1261 gatgagatta ttgattgtaa tcccaactct tttttcaatt ttgctaattg gtactcactc
     1321 ttaatgtaat tccacccttt acgccgagcg tacattgcag aacctgaacg aacatttacc
     1381 agtacatctg gtaaattata cacctcatat ttctgtgtaa taactcgtaa ccataaattg
     1441 taatcttcca tataaagatg atgctgatag cctccaacat gctcgatcac agatttttta
     1501 taggctactg ccatatggtt aaaaggattt cttagtaatg ctgaaatacg aatttcatca
     1561 tcattaatgg gcacttgttt tatacccaga gaattagaca ttgtttcgtc aaattcttca
     1621 acttgtcccc ccaataaaac cacatcagga tttttactaa taaaatcaat ttgcttttca
     1681 aatcgagatg gtaagcaaat atcatcagta tccattctaa acacccactc attagagcag
     1741 tgttttaagc cttcatttaa ggccggccta aacccacatt ttgtggtaaa ctaacaacaa
     1801 caagcggtaa tttttcagta aaatgttgca caattcgctc taaatctcca ggaattatac
     1861 catcaaaaac cacaacaatt tcacttgctg gtaaagtttg ggatactaaa gaatctaaac
     1921 acatctctaa ataagctggt ttttctttat aatacaaaga tattaatatc gaaaacttca
     1981 caactacttt tcccactttt
//
LOCUS       DANLRK010000255         1959 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.256, whole genome shotgun sequence.
ACCESSION   DANLRK010000255 DANLRK010000000
VERSION     DANLRK010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1959
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.256"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            130..711
                     /locus_tag="PXA33_002527"
                     /pseudo
     CDS             130..711
                     /locus_tag="PXA33_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252416.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
     gene            712..882
                     /locus_tag="PXA33_002528"
     CDS             712..882
                     /locus_tag="PXA33_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HDL5121811.1"
                     /translation="MLKCKQITELVSLSQEQKLTLKQRVNVQVHLMLCSRCRAFSQNC
                     KTVDKLMKEFNS"
     gene            complement(931..1899)
                     /locus_tag="PXA33_002529"
     CDS             complement(931..1899)
                     /locus_tag="PXA33_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS1 family transporter"
                     /protein_id="HDL5121812.1"
                     /translation="MCLKLLFGFDNLPVVYVLFTVIQVGLSINGFKGIKWLENVSCVF
                     LIGILAYMLYVVNTKFSVEIGERFANIEGTWGMPFWAATTAFLGIYSTMILNASDYSR
                     HVKKGTTPTATGVIYVLSILPVTLFMGLIGLLVTAATGNSDPIEVFATAMDNKFLTVI
                     TLLFIAFAQVTTNVLNNIVPPSYILMDSFKMKWSHATILVGILSICVMPWKLVTAESA
                     DGLSLFIKIYSAFLGPIFAVMVVDYYILRKRTLNVNDLYNKEGIFKGINWAAIIAIAI
                     GSFCSLIVVDLSWYVSLIPSGLSYYILMRVLKSSEAFRKGTVLEHQ"
BASE COUNT          681 a          366 c          350 g          562 t
ORIGIN      
        1 acccgcccct acgattagta tgttaggttt aaaaatactt cataccaata tcccctaagt
       61 tgggtaaaac cttatgaaat aggatacaag cggttagttt ctctcaattt tttaccaaag
      121 gatcacgcaa tgagccaacc ggctttacgg caaatttccc cctcgcagct agaagatatt
      181 cgtctgcaaa tggtgaagtt tgccttttta cagttaaaaa ataacgaact tgccgaagat
      241 gtggtgcaag aggcgtttgt cagcgcttat aaatatgccg acagcttcaa aggtgaaagt
      301 gcgttaaaaa cgtagatttt tgcaatttta aaaaacaaga tcgtagattt agtgaaagcc
      361 aaaggcaaat tggtggcagt atctgagctg atggaagaag acgatcacga tcttgccgat
      421 aaattgttta acgaaaatgg cggttgggat aaagatgctt atcaatctaa tgagtggaag
      481 aacaccgaaa gccataccta ttccgctcaa ttctggcaag tatttgaatt ttgcctaaat
      541 aatctgccgg caaatcaagc aagggtgttt atgatgcgtt cccatttgga aatggaaacc
      601 gaagaaattt gcgcagaatg tgagatttca ccagcaaatg tgcatacctt actgtatcgg
      661 gcaagattgc agttacaggt ctgtttaagt caaaaatggt ttggaggtta aatgttaaaa
      721 tgtaagcaaa ttaccgaatt agtttctctg tcccaagaac aaaaactgac attaaaacaa
      781 cgtgttaatg tgcaagttca tttaatgctt tgttctcgtt gcagagcctt tagccaaaac
      841 tgcaaaaccg tagataagct aatgaaagaa tttaactctt agcgtaaaag caagcggtaa
      901 aatttttaat aaattttacc gcttgtcatc ttattgatgt tctagcactg tccctttacg
      961 gaaagcttcg gaacttttta acaccctcat caaaatatag tagctcaagc ctgatggaat
     1021 taaactgaca taccaagaga gatccaccac aatcagtgag caaaacgaac cgatagcaat
     1081 cgcaataatt gctgcccagt taatcccttt gaaaatacct tctttattat ataagtcatt
     1141 tacgtttaaa gtacgttttc ttaagatgta gtaatcgacg accattaccg caaaaatagg
     1201 gcctaagaat gccgagtaaa ttttaataaa caaagataat ccatctgctg attcagcagt
     1261 taccaatttc caaggcatta cacatataga aagaatgccc actaaaatgg tggcatgcga
     1321 ccatttcatt ttaaatgaat ccattaaaat gtaagatggc ggaacaatat tattcaacac
     1381 gtttgtcgta acctgtgcaa aagcaatgaa taataacgtt attactgtta aaaatttatt
     1441 atccattgca gttgcaaata cttcaattgg gtcactattg cctgtagctg ccgtaactaa
     1501 caaccctatt aaacccataa ataaggttac agggaggatt gataatacat aaatcacacc
     1561 tgttgctgtt ggggttgttc cttttttaac gtggcgagaa taatcggaag catttaaaat
     1621 cattgttgaa taaataccca agaatgctgt agttgcagcc cagaatggca ttccccaagt
     1681 gccttcaata ttggcaaagc gctcgcctat ttctacagag aattttgtat tcacaacata
     1741 aagcatataa gcaagaatac cgatgaggaa cacacaagaa acattttcca accatttaat
     1801 acctttaaaa ccattaatgg ataagccaac ttgaataaca gtgaatagca cataaacaac
     1861 aggcaagtta tcaaaaccga acaacagttt taaacacata ttgacagcta ttttttgcgt
     1921 tttatcattt aattcaaaaa ttaaattaaa tctatttgc
//
LOCUS       DANLRK010000256         1855 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.257, whole genome shotgun sequence.
ACCESSION   DANLRK010000256 DANLRK010000000
VERSION     DANLRK010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.257"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(86..244)
                     /locus_tag="PXA33_002530"
     CDS             complement(86..244)
                     /locus_tag="PXA33_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121813.1"
                     /translation="MDTTQKRVALDTILQCLHFAEEKGAIPPIPEDWRYEMTGRSMGI
                     EQIPNQKL"
     gene            complement(248..424)
                     /locus_tag="PXA33_002531"
     CDS             complement(248..424)
                     /locus_tag="PXA33_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121814.1"
                     /translation="MAYVRIYQGEAYGWCAEKGEANTECPEALLVSLTGDIFEAVGGD
                     KLKGAKKWQPYKKE"
     gene            438..695
                     /locus_tag="PXA33_002532"
     CDS             438..695
                     /locus_tag="PXA33_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121815.1"
                     /translation="MICHADPEYVKYNYAGLWEKGSDGTFFYNYQQFMPEVEAKESLR
                     EQEPELDDGYNEEEYSEEEYSDENDASDENDNYDETYDDNF"
     gene            833..988
                     /locus_tag="PXA33_002533"
     CDS             833..988
                     /locus_tag="PXA33_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121816.1"
                     /translation="MLFKEKGYDEFLTQKIAKGRAELESGKGIPLEQATLLVQSTIEE
                     TAQELDD"
     gene            1042..1200
                     /locus_tag="PXA33_002534"
     CDS             1042..1200
                     /locus_tag="PXA33_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121817.1"
                     /translation="MTEEYQNFLIEQVKQGLDDIENGRVISLEESRQLCQKTIYETLQ
                     ELQGAVYA"
     gene            1193..1510
                     /locus_tag="PXA33_002535"
     CDS             1193..1510
                     /locus_tag="PXA33_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL5121818.1"
                     /translation="MLSRKEVLMTRRASMNLQQIIRNVVEYTYSYESGDKLSAEIHNT
                     LDNLSYFPRIGVIGSVENTRECFCRGYRIIYRETENQVQILAIIHCRQQYPTDYYRSA
                     FKA"
     gene            1631..>1855
                     /locus_tag="PXA33_002536"
     CDS             1631..>1855
                     /locus_tag="PXA33_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121819.1"
                     /translation="MSLLKKAQTFISIFNEVRAKNEAQRQSSNNLNVALKNAAEMRAE
                     MNGFSLKPQKRSTYFADHYGCGIHAVETYDN"
BASE COUNT          648 a          362 c          322 g          523 t
ORIGIN      
        1 taaacaatta ttcataataa aaaagtatgg ttagctaaaa tttagccaca ccatactttc
       61 ttttgtgtat tttgtaaatg cccagctata atttctgatt tggaatctgc tcaatcccca
      121 tactacgccc cgtcatttca taccgccaat cttcaggtat aggcggtata gctcccttct
      181 cttcagcaaa atgcagacac tgcaatatag tatctaaagc cacacgtttt tgtgtcgtat
      241 ccatctgtca ttctttcttg taaggctgcc attttttggc acctttcagc ttatctccgc
      301 cgactgcttc aaaaatatcg ccggttaaac tcaccaataa ggcctcaggg cattctgtat
      361 ttgcctctcc tttttcagca caccaaccat aagcctcacc ttggtaaata cgaacatatg
      421 ccacccctca attagggatg atatgccacg ctgatcctga atatgtaaaa tacaactacg
      481 ccggactttg ggaaaaaggc agcgatggca cttttttcta caattaccag caatttatgc
      541 ctgaagttga agctaaagaa tcactaaggg aacaagaacc tgaacttgat gatggatata
      601 acgaagagga atatagcgaa gaagaatatt ccgatgaaaa tgatgccagc gatgaaaacg
      661 ataactacga tgaaacttat gatgataatt tttaaatatt cccaaaccac acttccaaaa
      721 accctgctct tgacactcta ttgctttaag cgtaaattat tccaatattt cgcatttagg
      781 agaacataat ccctatgcct gaaagccttg taataagtga aaggaacata aaatgctatt
      841 taaagaaaaa ggctacgatg agttccttac tcaaaaaatt gcaaaaggca gagctgaatt
      901 agaatccgga aaaggtattc ctcttgagca agccacattg ttagttcaat caactattga
      961 agaaactgct caagaactgg acgattaaca atttgtaaca ttgctaatat atctatatac
     1021 aactaatgat aggagttatc catgacagaa gaataccaaa acttcttaat agagcaagta
     1081 aaacaaggac ttgatgatat agaaaacgga cgtgttattt cattagaaga aagccgtcaa
     1141 ctgtgtcaaa aaaccatata tgaaaccttg caagagctac agggagccgt gtatgcttag
     1201 tcgaaaagag gtgttaatga ctcgcagagc aagtatgaat ttacaacaaa tcattcgtaa
     1261 tgttgtggaa tatacttata gctatgaaag tggtgataaa ttatctgcgg aaattcataa
     1321 cactttggat aatctttctt attttccacg aattggtgtt ataggttcag tagaaaatac
     1381 tagagaatgc ttttgccgag gttatagaat tatatatcga gaaacagaaa atcaagtgca
     1441 aattttagcc attatccatt gtcgtcaaca atacccaacc gactattatc gctctgcatt
     1501 caaagcataa caggagctat aaaatggaaa taaaaagaaa aatgaccgta gccgaattaa
     1561 aactcaaggc aatgctcgcc aagcctacca tagttcaata atattaggtg caaaaaacag
     1621 gagaaccaca atgtctctac ttaaaaaagc acaaacgttt atctctattt ttaatgaagt
     1681 gcgagctaaa aatgaagctc aacgccagtc ttcaaacaat ttaaatgttg ccttgaaaaa
     1741 tgctgctgaa atgagagctg agatgaatgg gttctccctt aagcctcaaa agcgttctac
     1801 ttactttgct gaccattatg gttgtggtat tcatgcagta gaaacctatg ataat
//
LOCUS       DANLRK010000257         1804 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.258, whole genome shotgun sequence.
ACCESSION   DANLRK010000257 DANLRK010000000
VERSION     DANLRK010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.258"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            <1..1539
                     /locus_tag="PXA33_002537"
     CDS             <1..1539
                     /locus_tag="PXA33_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase/relaxase domain-containing protein"
                     /protein_id="HDL5121820.1"
                     /translation="VHDIGKVVVDIEITLKDGSRWFAWNGQPPQPYKFKYIKGRDYHL
                     HPVLGGFLANQLIPKSAFDWLGQYPEVISSLMYAMSGHNDKAGLLSEIVQKADQHSVT
                     LAIGGDVNKLVQRPVNSLPKQIVLALRYLLEQKIKLNTPKGPADGWFTEDGLWLVSKT
                     AADNIRTYLLGQGISVPKDNPKLFDEMQSLGIVEESPSGGAIWSCRIQADSGWTPATT
                     FTLLRVKPEVAWENINDRPAYFAGKVKVIEQITEEVNAEVQPPLVEETTKISLLPSKQ
                     SEAIIPSEETEISKAITSSDNATSAEVIINNENDLTSFVLDMFAPTETIEQNISVISS
                     NKEQQIVMTTLTEQEKVPPKKVKLEKSKKKNLQLTTLEPWNDMINPITGKEFVNWLKE
                     GIATNKFAINKPSAKLHIVDGNLFLVTPVIFEMYLQECGQPYDKETINNLQYDFQGLG
                     LHSLKKTMTNGKPDTINFWKCDVIGPRKTSSLTGYLIKNTHLLFGDKMLLNNHCLTLQ
                     EEDT"
     gene            1579..>1804
                     /locus_tag="PXA33_002538"
     CDS             1579..>1804
                     /locus_tag="PXA33_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase SAM-like domain-containing
                     protein"
                     /protein_id="HDL5121821.1"
                     /translation="MFFYRHYLRADTKRSYNNIIRIIKKRYPNLNANDVTTDMLIEWR
                     NQVVGNTIKAVTWNNYVRHLKALYNFAIAQG"
BASE COUNT          601 a          313 c          374 g          516 t
ORIGIN      
        1 gttcatgata ttggcaaagt tgtggttgat attgaaatca ctcttaaaga cggctctcgt
       61 tggtttgctt ggaatggaca gccacctcaa ccttataaat tcaaatacat caaaggtcga
      121 gactaccatc ttcatccggt gctaggagga ttccttgcaa atcagctcat tcccaagtct
      181 gcgtttgatt ggctgggaca gtatccagaa gtcatttctt cactgatgta tgcaatgtct
      241 gggcataacg ataaagccgg gcttttatct gagatcgtgc agaaagccga tcagcatagt
      301 gttacgctag caatcggtgg cgatgtgaat aagctggtac agcgaccagt taattcattg
      361 ccgaagcaaa ttgtgctggc tctacgttat ttactggagc agaaaatcaa attaaatacg
      421 ccaaaaggac cagcagacgg ctggtttacc gaagatgggc tgtggctggt aagtaaaact
      481 gctgccgata atatccgaac ttatttactg ggacagggga tttccgtgcc taaagataat
      541 ccaaaactgt ttgatgaaat gcaatctctt ggtattgtgg aagagtctcc ttccggcggt
      601 gcaatttgga gctgccgtat ccaagccgat tccggttgga caccggcgac aacatttacg
      661 ctattacgag ttaaaccgga ggttgcttgg gagaatatca acgatagacc ggcgtatttt
      721 gccggaaaag tcaaagtgat tgaacagata actgaagagg tcaatgcaga ggttcagcct
      781 ccattagttg aagaaaccac taaaatttct ctattaccga gcaagcaatc ggaggctatt
      841 attccgtctg aggaaacgga aatatcgaag gcaattacaa gttcagacaa tgcaacatct
      901 gcggaagtaa tcataaataa tgaaaatgat ttaacttctt ttgtattgga tatgtttgct
      961 cctacagaga caattgagca aaatatttct gtcatttctt caaataaaga gcagcaaatc
     1021 gtaatgacaa cacttacgga acaagaaaaa gttccgccga aaaaagttaa attagagaaa
     1081 agtaagaaaa aaaatcttca attaaccact ctagaaccat ggaatgatat gattaatcca
     1141 attactggaa aagaattcgt aaattggttg aaggagggga tagcaaccaa taaatttgca
     1201 attaacaaac cttctgcaaa attacatata gtagatggta atttattttt agtcacacca
     1261 gttatttttg aaatgtattt gcaagaatgt ggccaacctt atgataagga aactataaac
     1321 aatcttcagt atgatttcca ggggcttggt cttcattctc ttaaaaaaac catgactaat
     1381 ggtaagccag atacaataaa tttttggaag tgtgacgtga ttggtcctag aaagacttcc
     1441 tctttaacag gatatttgat aaaaaatact catctcttat ttggggataa aatgttacta
     1501 aataaccact gtttaacttt acaggaagag gacacataat atggattcta agatttcctt
     1561 cgatatatta ttggaacgat ttttttctat cgtcattatt tacgggcgga cacgaaacga
     1621 agttataaca atattattcg aattatcaaa aagcgttatc caaacttaaa tgcgaatgat
     1681 gtgactactg atatgttaat tgaatggcgt aatcaagttg ttggtaatac aattaaagct
     1741 gtgacgtgga ataattatgt gagacaccta aaagcattat ataattttgc gatagcacag
     1801 gggc
//
LOCUS       DANLRK010000258         1790 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.259, whole genome shotgun sequence.
ACCESSION   DANLRK010000258 DANLRK010000000
VERSION     DANLRK010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.259"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            59..832
                     /locus_tag="PXA33_002539"
     CDS             59..832
                     /locus_tag="PXA33_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FNR family transcription factor"
                     /protein_id="HDL5121822.1"
                     /translation="MKIVSEPKTSGRHTCTIHCQNCSISQLCLPFTLNETELTQLDNI
                     IERKKPIQKSQVIFKLGDELRSLYAIRSGTLKAYTLSESGEEQITGFHLPGDLIGFDA
                     ITNMQHEGYAQALETSMICEIPFDILDDLAGKMPKIRHQIMRLMSNEIKSDQEMILLL
                     SKMNAEEKLAAFLYNLSQRYSARGFSAKEFRLTMTRGDIGNYLGLTIETISRLLGRFQ
                     KSGMISVQGKYIIINRMEELAELAGAIKPQQNIIAQTTA"
     gene            976..1767
                     /locus_tag="PXA33_002540"
     CDS             976..1767
                     /locus_tag="PXA33_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL5121823.1"
                     /translation="MGILTGKRILVTGLASNRSIAYGIANAMKQQGAELAFTYLNDKL
                     KPRVEEFAKEFGSDIVLPLDVATDESITECFTELSKHWEKFDGFVHAIAFAPGDQLDG
                     DYVNAATREGYRIAHDISAYSFVAMAQAARPFLNENAALLTLSYLGAERAIPNYNVMC
                     LAKASLEAATRVMAADLGKEGIRVNAISAGPIRTLAASGIKNFKKMLSAFEKTAALRR
                     TVTIDDVGNSAAFLCSDLASGVTGEVLHVDAGFSVMAMGELGDEE"
BASE COUNT          557 a          359 c          346 g          528 t
ORIGIN      
        1 tggtaaactt tgttctaaat caaatttagg aatacttttc taatttaagg ttgaatggat
       61 gaaaattgta tcagaaccta aaacaagcgg acgtcatact tgcacaattc attgtcaaaa
      121 ttgcagtatt agccaactat gcttaccttt tactcttaat gaaacggaat taactcaact
      181 agataatatt attgagcgta aaaaaccgat tcaaaaatcg caagttattt ttaagttagg
      241 tgatgaactg cgttcgcttt atgctatccg ttctggaaca ttaaaagcct ataccttaag
      301 tgaaagtggt gaagagcaaa ttaccggttt ccatttacct ggcgatctaa ttggctttga
      361 tgcaattacc aatatgcagc acgaaggcta tgctcaagca ctcgaaacct caatgatttg
      421 tgagattcca ttcgatattt tagatgatct tgccggcaaa atgccaaaaa ttcgccatca
      481 aattatgcgt ttgatgagta atgaaattaa aagcgatcaa gaaatgattc tattgctctc
      541 aaaaatgaat gcggaagaaa aactagctgc atttttgtat aatctttccc aacgttactc
      601 agcccgtggt ttctctgcaa aagagttccg tctaactatg acccgtggag atatcggcaa
      661 ttatcttggt ttaaccattg aaactatcag ccgtttatta gggcgtttcc aaaaaagcgg
      721 tatgatttca gtacaaggaa aatatattat tattaaccgt atggaagaat tagccgaatt
      781 agccggggca atcaaaccgc agcaaaatat tattgctcaa actaccgctt aatttatctt
      841 accaaatccc tttttgaaac ctcgttcaaa aagggatttc tgatccgatg agccgctaac
      901 tccatttgcc cgtttttcct atatccgtta taatcgacac aattttgccc caatcaatat
      961 aaaagaggaa tttttatggg tatcttaact ggtaaacgta ttttagtgac aggtttagca
     1021 agcaaccgtt cgatcgctta cggtattgca aacgcaatga agcaacaagg tgcagaactt
     1081 gcattcacct acttaaacga taaattaaaa ccacgagttg aagaatttgc caaagaattt
     1141 ggctcagaca ttgtccttcc gttagatgta gcaaccgatg aaagcattac tgagtgcttt
     1201 actgaattaa gcaaacattg ggaaaaattt gacggttttg ttcacgctat cgcatttgca
     1261 ccgggcgatc aattagacgg cgattatgta aatgccgcaa cccgtgaagg ttatcgtatt
     1321 gcacacgaca tcagtgccta tagctttgtt gcaatggctc aagctgctcg ccccttctta
     1381 aatgaaaatg ccgccctttt aaccttaagt tacttaggtg cagagcgtgc aatccccaac
     1441 tacaatgtaa tgtgcttagc caaagcctct ttagaagctg caacccgtgt aatggcagcc
     1501 gatttaggta aagaaggtat tcgtgtaaat gcgatttcag ccggcccgat ccgtacatta
     1561 gctgcttcag gcattaaaaa cttcaagaaa atgctttctg ctttcgagaa aaccgctgct
     1621 ttacgccgca ccgtaactat tgatgatgta ggtaactctg cagctttctt atgctcagat
     1681 ttagcctctg gtgtaacagg tgaagtgtta cacgttgatg ccggcttcag cgtaatggca
     1741 atgggtgaat taggcgacga agagtaattc ctataaaaat aacggactca
//
LOCUS       DANLRK010000259         1779 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.260, whole genome shotgun sequence.
ACCESSION   DANLRK010000259 DANLRK010000000
VERSION     DANLRK010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.260"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            <1..493
                     /locus_tag="PXA33_002541"
     CDS             <1..493
                     /locus_tag="PXA33_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL5121824.1"
                     /translation="ARPLNTLEVKGLTVVDDSQKPLFSEVNQALYHGLSPIEVVASRV
                     QITRAITTYTKNVTNTDDPSYLDLTTIRTLDYVRKAIQTRQRLRFPRAKNSHRIVAKV
                     RSEILDVLYQLEGEEVIENVEAWKNRLLVVRNQDPTYLDLEIPADVVNGLHVIRNKIT
                     LIL"
     gene            504..878
                     /locus_tag="PXA33_002542"
     CDS             504..878
                     /locus_tag="PXA33_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL5121825.1"
                     /translation="MSIVYEGSCVLEVDGVEIEITKLDVKTVTGRKLVKTMNSSGRAR
                     GYSQGIAEYTLSVTAVEPQDGSVIDWDNIKNAKLTKYPLDNSEKRTSYLGCFTTDVSE
                     SYTVDGESVRDIQLNALRKVNE"
     gene            878..1246
                     /locus_tag="PXA33_002543"
     CDS             878..1246
                     /locus_tag="PXA33_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121826.1"
                     /translation="MKITLNLGIEYNGKIRRDCTVRLLTMGGQCTAQEMIADLGFEEN
                     SEALSKREQTLVDMAYLSQQVTFDGIYQGNVTAQFLFDNLATEDYWQLIEATLTLRKK
                     RLENGVSQSESESPANPDLA"
     gene            1276..1464
                     /locus_tag="PXA33_002544"
     CDS             1276..1464
                     /locus_tag="PXA33_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121827.1"
                     /translation="MILLSKFAITAEQVWQMPLLEVFAWIESYLESEGVKTPHSPMGA
                     NSEVKRESYVFTRCGKIE"
     gene            1517..>1779
                     /locus_tag="PXA33_002545"
     CDS             1517..>1779
                     /locus_tag="PXA33_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121828.1"
                     /translation="MAKNMKVQLELTAKDTASPVLNQAAKTAEKAFKNTERAAQKSGN
                     AQTQAAQKAANATESSNRKIEQSYRNARRVAADVSRARETLGVR"
BASE COUNT          540 a          352 c          447 g          440 t
ORIGIN      
        1 ggctcgcccg ttaaataccc ttgaagttaa aggcttaacg gtcgtagatg actcgcagaa
       61 accgttattt agcgaagtaa accaagcgtt atatcacggt ttaagcccga ttgaggtggt
      121 ggcaagccgt gtgcagatta cccgtgcgat tactacatac accaaaaacg tgacgaatac
      181 tgatgaccca agctatttgg atttaaccac cattcgtacg cttgactatg tgcgtaaggc
      241 aatccaaacc cgtcagcgtt tgcgtttccc tcgtgcgaaa aactcccacc gtattgtggc
      301 aaaagtacgc tcggagattt tagatgtgct ttatcagctt gagggcgaag aggtaatcga
      361 aaatgtggaa gcgtggaaaa atcgcttatt agtggtgcgt aatcaagacc cgacttactt
      421 ggatttagaa atccctgcgg atgtggttaa tggcttgcac gttattcgta acaaaattac
      481 gttaatttta tagggggtgc tagatgtcaa ttgtttatga aggctcttgc gtgcttgagg
      541 tagatggcgt tgagattgaa attaccaaac ttgatgtaaa aaccgtaacc ggtcgcaagc
      601 tggttaaaac gatgaacagt tccggacgtg caagaggtta ttcgcaaggc attgcggaat
      661 atactctctc ggtgactgca gttgagcctc aagacggttc ggtaattgat tgggataaca
      721 ttaaaaatgc gaagctcact aaatacccgt tagacaatag cgaaaaacgc acctcttatt
      781 taggctgttt tactaccgat gtgagcgagt cttacaccgt tgatggtgag tcggtgcgtg
      841 atattcaact caacgcattg cgtaaggtga atgaataatg aaaatcactt taaatcttgg
      901 cattgaatat aacgggaaaa ttcgccgtga ttgtacggtg cgattgctga cgatgggcgg
      961 tcaatgcacg gctcaagaaa tgattgcgga tttagggttt gaggaaaact cggaagccct
     1021 atcaaagcgt gaacaaacct tggtcgatat ggcttacctt tcgcagcaag ttacctttga
     1081 tggtatttat caaggtaatg tgacggctca atttttattt gacaatttag ctaccgaaga
     1141 ctactggcag ttgattgagg caacgctcac actccgaaaa aagcggttag aaaatggggt
     1201 gagccaaagc gagagcgaaa gcccggcaaa tcccgatttg gcgtagcaga agcctttaag
     1261 cagtaccgac aagcggtgat tttactctca aaatttgcaa ttaccgccga gcaggtatgg
     1321 caaatgccgt tgcttgaggt atttgcgtgg attgagagct acttggagag tgagggcgta
     1381 aaaacaccgc atagcccaat gggtgcgaat agcgaagtga aacgcgaaag ctatgtcttt
     1441 actcggtgtg gcaaaattga ataggggcga aagccccttt tttgtacttt aaaatgagtt
     1501 taaagagact ttaaaaatgg caaaaaatat gaaagtgcaa ctggaattga cggcaaaaga
     1561 tactgcctcc ccagttttaa accaagccgc taaaaccgcc gaaaaagcct ttaaaaatac
     1621 agagcgagcg gcacaaaaaa gtggcaatgc tcaaacgcaa gcagcacaga aagcagcgaa
     1681 tgctacagaa agctcaaatc ggaaaataga acagagttac cgtaatgccc gccgagtggc
     1741 tgccgatgtg agccgagccc gtgaaacatt aggtgttcg
//
LOCUS       DANLRK010000260         1741 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.261, whole genome shotgun sequence.
ACCESSION   DANLRK010000260 DANLRK010000000
VERSION     DANLRK010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.261"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            <1..218
                     /locus_tag="PXA33_002546"
     CDS             <1..218
                     /locus_tag="PXA33_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121829.1"
                     /translation="KNLTDESEEQAILAYLTENIMARIEKEQPLEKTLILYSEQLASF
                     EQKSPMALHREKFEAKPTGLAELINNL"
     gene            229..1179
                     /locus_tag="PXA33_002547"
     CDS             229..1179
                     /locus_tag="PXA33_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P2 family phage major capsid protein"
                     /protein_id="HDL5121830.1"
                     /translation="MSLSTFAHALKLDKQGNVILFDDSPEEQAKLISRLKQNRLFKLL
                     NLVQTKHAKGEAIGLFTPIAGTTNTESGEEREPKQALAPAQKYHCEQINIDSYIRYEK
                     IDAYNLENSLEERLNQLLDDNLLRGLLMVGWQGVSRADTSNATNNQLAQDVKKGWLQK
                     IREGKPEAVINGATIGKEQQYKSLNALIKQGLGKISPAYAMGGDMIAICGRDIIADDV
                     IQLEHNDLSENLADLLTLCQQAKGGLKAVSIPYFPSNAILITRLDNLSLYLHLNNIRR
                     KAFDTPQKDSVETFYSLNVDYIVEDFNACCLIENIEVIEE"
     gene            1176..1370
                     /locus_tag="PXA33_002548"
     CDS             1176..1370
                     /locus_tag="PXA33_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121831.1"
                     /translation="MSLESYIQQAEEMELLEKLDFVSDLLELEKVSEIEAYRILTAHG
                     EPKPQPAQEKLIGCVDFLDD"
     gene            1488..>1741
                     /locus_tag="PXA33_002549"
     CDS             1488..>1741
                     /locus_tag="PXA33_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121832.1"
                     /translation="MTNYKLPARKWYTLEQATKRIKQLTGEDIEIEDLLHYWYLRKLE
                     LSIYLNRNGNGDLIIGNHSFNKEEYIVLEQPQIIIREDGS"
BASE COUNT          655 a          343 c          328 g          415 t
ORIGIN      
        1 ataaaaacct cacagatgaa agtgaagagc aagctattct ggcatatcta acagaaaata
       61 taatggctag aattgaaaag gaacagccac ttgaaaaaac attaattctt tattctgaac
      121 agttagcaag ttttgaacaa aaatcgccaa tggcattaca ccgtgaaaaa tttgaagcaa
      181 aacctaccgg cttagctgaa ttaattaata acttataaga ggtaaaaaat gagtttatcc
      241 actttcgccc acgcattaaa actagataaa caaggtaacg ttattttatt tgacgatagc
      301 cccgaagaac aggcaaaact aattagccgc ttaaaacaaa atcgcctttt taaattattg
      361 aatttagtgc aaaccaagca cgcaaagggc gaagcgattg ggctattcac accgatagcc
      421 ggcacaacca acaccgaaag cggagaggaa cgagagccaa aacaagccct tgcacccgct
      481 caaaaatacc actgcgaaca aatcaacatt gatagctata ttcgctatga aaaaatcgat
      541 gcttacaacc tcgaaaacag cttagaagaa cgcttaaatc agttacttga tgacaacctt
      601 ctaagagggc ttttaatggt tggctggcaa ggcgtaagcc gtgcagatac atcaaacgcg
      661 acaaacaacc aactcgccca agacgtgaaa aaaggctggc tacaaaaaat cagagagggc
      721 aagcccgaag cggtaattaa tggagcaacc ataggcaaag agcaacaata caaaagccta
      781 aatgctctga ttaagcaggg attaggcaaa attagcccag cgtatgcaat gggcggagat
      841 atgatcgcca tttgtgggcg ggatattatc gctgatgatg taattcagct tgaacacaac
      901 gatttaagcg aaaacctagc cgacttatta accttatgcc aacaagctaa aggcggacta
      961 aaagcggtaa gtattcccta cttcccaagc aatgcgattt taatcactcg attagataac
     1021 ctttcgctat atcttcattt aaacaatatc cgccgtaaag cgtttgatac gccacaaaaa
     1081 gacagcgtag agacatttta ctccctcaat gtggattaca ttgtagaaga ttttaacgcc
     1141 tgttgtttaa ttgaaaatat tgaggttatt gaagaatgag cctagaaagc tatatccaac
     1201 aagcggaaga aatggagctt ttagaaaaac tcgattttgt ttcagacctg ttagaacttg
     1261 agaaagtcag cgaaattgaa gcctatcgca ttttaaccgc ccacggcgaa cctaaacccc
     1321 aaccggcaca agaaaagcta ataggttgcg tagattttct tgatgactaa cccaagcccc
     1381 gaaaggggct ttttgcattt ttgcaacaaa aaacgaccgc ttgcaccctt tgaaattatt
     1441 gctatcatta ataaaaaaca ataaccaaat caaatgagag tacagcaatg acaaactata
     1501 aactaccggc tagaaagtgg tacaccttag agcaggcaac aaaacgcatt aaacaactca
     1561 ctggcgaaga tatagaaata gaagatttat tacattattg gtatttaaga aagctagaat
     1621 taagtattta tttaaataga aacggaaatg gcgatctgat tataggcaat cattctttta
     1681 ataaagaaga atatattgtc ttggaacaac cacaaataat cataagagaa gatggctcta
     1741 t
//
LOCUS       DANLRK010000261         1716 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.262, whole genome shotgun sequence.
ACCESSION   DANLRK010000261 DANLRK010000000
VERSION     DANLRK010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.262"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            <1..324
                     /locus_tag="PXA33_002550"
     CDS             <1..324
                     /locus_tag="PXA33_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDL5121833.1"
                     /translation="LASALENLIRNGQKYAKKMLSVSMNVEDGHLFLTVEDDGEGVPE
                     NEYENILKPFYRVDEARARETGGAGLGLAIVQNVVKQHQGTIHLDKSNMGGLKIELKL
                     PLWRS"
     gene            374..>1716
                     /locus_tag="PXA33_002551"
     CDS             374..>1716
                     /locus_tag="PXA33_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL5121834.1"
                     /translation="MKLIEIENLNKYFGLGQTRAHILKNINLSIERGDFVAIIGASGS
                     GKSTLMNIIGCLDTASSGICRIDGKETQTMSADELSDLRQRKLGFIFQRYNLLSALNA
                     NENVALPAIYAGMDSSSRKKRADTLLTKLGLSDKTQHKPNELSGGQQQRVSIARALMN
                     GGEIILADEPTGALDSKSGETVLEILRDLHYEGHTIIMVTHDPNIAAQASRIVEIKDG
                     QIIRDERQRTDVQETALNKTTTQKPRFWNQLFESFKMATSAIMAHKMRAILTMLGIII
                     GITSVISVVALGRGSQQQILSNINNLGTNTMDIMNGTGFGDRRANLTKNLTISDALML
                     SRQNYVESTTPSSNVSATLIYGNTAVTGSVRGVGEQFIDVKGLKLISGRFFSAEEVKN
                     VAQVIVIDPNTQKDLGLTEPVEGKTILVDKKPLQIIGVAQQSNNMNQSSLTLWAP"
BASE COUNT          568 a          332 c          371 g          445 t
ORIGIN      
        1 ctggctagtg ctttagaaaa tttgatccga aacggccaaa aatatgcaaa aaaaatgctg
       61 tcggtctcaa tgaatgtaga agatggtcat ttatttctca cagttgaaga tgatggcgag
      121 ggggttcctg aaaacgaata tgaaaatatt cttaaacctt tttaccgcgt agatgaagca
      181 agggcaagag aaacaggagg agccgggcta ggattagcga ttgtgcaaaa tgtagttaaa
      241 cagcaccaag gtactattca tttagataaa agtaatatgg gaggattaaa aatcgaactg
      301 aaacttcccc tttggcgttc ctaaggcaaa aagcatatcg tttgtaaaaa acagtcgaaa
      361 aataaccgct tgtatgaaat taattgaaat agaaaatctc aataaatact ttggcttggg
      421 gcaaacccga gcccatattc taaaaaatat caatctcagt attgaacggg gtgattttgt
      481 cgcaattata ggtgcttcag gttctggcaa atctacctta atgaacatta ttggctgttt
      541 agatactgcc agctcaggta tttgccgtat tgatggcaaa gagacccaaa caatgtctgc
      601 agatgaactg tctgatttac gccaacgtaa actggggttt attttccaac gctataatct
      661 cctttcagct ttgaatgcaa acgaaaatgt tgccctacct gcaatttacg ccggaatgga
      721 tagctcatca cgcaaaaaac gagctgatac gttattaacg aagttaggat tatcagataa
      781 aacccaacat aagccgaatg agttatctgg ggggcaacag caacgagtca gcattgcacg
      841 agccttaatg aacggcggtg aaatcattct tgcagacgag cctaccggtg cattagattc
      901 taaaagtggc gaaactgtat tggaaatctt gcgagatcta cactacgaag gtcataccat
      961 tattatggta acacacgacc caaacattgc cgctcaagcc agccgtatcg ttgaaattaa
     1021 agatgggcaa atcattcgag atgaacgcca acgtactgat gttcaagaaa cggctctcaa
     1081 taaaacgaca acacaaaaac ctcgcttttg gaatcaactg tttgaatcct tcaaaatggc
     1141 aaccagtgca attatggcgc ataaaatgcg ggcaatcctc acgatgctgg gcattattat
     1201 tggcattacc tctgtgattt ccgtagtcgc tctcggtcga ggctcgcaac aacaaatcct
     1261 ttctaatatc aacaacctag gcacaaatac gatggatatt atgaatggaa ccggctttgg
     1321 cgatagaaga gccaatctga ctaagaattt aaccatcagt gatgccttaa tgttgagtcg
     1381 gcaaaactat gtggaaagca ctactccttc aagcaatgtc agtgccacat tgatttatgg
     1441 taataccgca gtaaccggtt cagtacgagg tgttggagag caatttattg atgtgaaagg
     1501 attaaagctg atttcagggc gctttttcag tgccgaggaa gtaaaaaatg tagcgcaggt
     1561 tattgtaatt gaccctaaca ctcaaaaaga tttggggcta acagagcctg ttgaaggtaa
     1621 aacgatttta gtggataaaa aaccgttaca aatcattggt gtggcgcaac agtcaaacaa
     1681 tatgaaccaa agtagcctta ccttatgggc gcctta
//
LOCUS       DANLRK010000262         1703 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.263, whole genome shotgun sequence.
ACCESSION   DANLRK010000262 DANLRK010000000
VERSION     DANLRK010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.263"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            <1..1642
                     /locus_tag="PXA33_002552"
     CDS             <1..1642
                     /locus_tag="PXA33_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HDL5121835.1"
                     /translation="DLNADTSQAFLNELDQTLWTAADKLRKNLDAANYKHIVLGFIFL
                     KYISDSFTDFQAKLKTQLTTPESELYLDPALFDEQEFSQILAEELEQRDYYAAENIFW
                     VPEQARWDNIKSLSKLNLGDELPWGDKFKGVSRLIDDAFEAIERENPKLKGVLQRIAG
                     FGVPDEMLTGLIDLFSRTNFTQPMHNGEPVHLQAKDILGHVYEYFLGQFALAEGKKGG
                     QYFTPKSIVTLIVEMLEPYSGRIYDPAMGSGGFFVQADRFIQAHAGNRNAISVYGQES
                     NSTTRKLAVMNMAIRGIPFDFGDKPEDTLLNPLHIDKKMDVVMANPPFNQKEWWNESL
                     ANDPRWAYGTPPQGNANFAWLQHMIYHLSPKGKMALLLANGSMSSQTSGEGDIRKNIV
                     QADLVEAMIALPNQLFTNTQIPACIWIINKAKARKGEVLFINATQIGYLKDRVLRDFT
                     ADDIAKISDTYHNWQKQNGYENIPAFCYCATLDEIAKNDFVLTAGRYVGAVQEENDGV
                     PFAEKMQELTALLNEQFKQGRELEQQIAENLKGLGYGI"
BASE COUNT          512 a          442 c          363 g          386 t
ORIGIN      
        1 tgatctcaac gctgacacaa gtcaagcctt tttaaacgaa ctcgaccaaa ccctctggac
       61 tgccgccgac aaactgcgta aaaacctcga tgccgccaac tacaaacaca tcgttcttgg
      121 ctttatcttc ctaaaataca tctccgacag ctttaccgat ttccaagcca agctaaaaac
      181 ccagcttacc acccccgaaa gcgaactcta tcttgaccct gcactatttg acgaacaaga
      241 atttagccaa attcttgccg aagagttgga acagagagat tactacgccg ctgaaaacat
      301 cttttgggtg ccggagcaag cccgctggga caacatcaaa tcattaagca aactcaatct
      361 tggcgatgaa ttgccttggg gagacaaatt taaaggtgtc agccgcttga ttgatgatgc
      421 ctttgaagcc atcgaacggg aaaaccccaa actcaaaggc gtactccaac gcattgccgg
      481 ctttggcgtg cctgatgaaa tgctcacagg cttaattgac ctgttctcac gcaccaattt
      541 cacccagcct atgcacaatg gcgaacctgt gcatctgcaa gccaaagaca ttttagggca
      601 cgtctatgaa tactttcttg ggcaatttgc ccttgccgaa ggcaaaaaag gcggtcaata
      661 cttcacgcca aaatccatcg ttaccctgat tgttgaaatg ctcgaaccct attcagggcg
      721 gatttacgac ccagctatgg gcagcggcgg cttttttgtg caagctgacc gctttattca
      781 ggctcacgca ggcaaccgca acgccatttc cgtttatggg caagaatcca actccaccac
      841 tcgcaaactg gcggtgatga atatggcgat tcgtggtatt ccctttgact ttggcgacaa
      901 gcccgaagat accctactaa accctttgca catcgacaaa aaaatggatg ttgtgatggc
      961 aaatccgccc tttaaccaaa aagagtggtg gaatgaaagc ctagcaaacg atccacgctg
     1021 ggcatacggc acaccgccgc aaggcaacgc caactttgcg tggttgcaac atatgattta
     1081 ccacctctcc cccaaaggca aaatggcact cctgctcgcc aacggctcaa tgagcagcca
     1141 aacttcaggc gaaggcgaca ttcgcaaaaa catcgtgcaa gctgaccttg tcgaagcgat
     1201 gattgccctg cccaatcagc tattcaccaa cacccaaatc cctgcctgca tttggattat
     1261 caataaagcc aaagccagaa aaggtgaagt gctgtttatc aacgccaccc aaataggcta
     1321 cctgaaggac cgcgtcttgc gtgattttac cgctgatgac atcgccaaaa tcagcgacac
     1381 ctaccacaac tggcaaaaac agaacggcta cgaaaatatc cctgcgtttt gttattgtgc
     1441 cacgctggac gaaatcgcca aaaacgattt tgtgctgacc gcagggcgat atgtcggtgc
     1501 ggtacaagaa gaaaatgacg gcgtgccgtt tgcagaaaaa atgcaggaat tgaccgcttt
     1561 attgaatgaa caatttaaac aagggcggga attggaacag caaatcgcag agaatttaaa
     1621 ggggttggga tatggcattt aatcagtatg tattttcaga tattgttgaa ttaatatccg
     1681 aaaaaataaa aatcaaagac tta
//
LOCUS       DANLRK010000263         1691 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.264, whole genome shotgun sequence.
ACCESSION   DANLRK010000263 DANLRK010000000
VERSION     DANLRK010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.264"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            82..1035
                     /gene="msrP"
                     /locus_tag="PXA33_002553"
     CDS             82..1035
                     /gene="msrP"
                     /locus_tag="PXA33_002553"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HDL5121836.1"
                     /translation="MKKLTIQDVTPETIFYQRRKIITALGLGIAASALPKVSMAETQR
                     HLQSLQFKPDPNKGLELTPENKVIGYNNFYEFGVDKSAPAKNAANFKTDPWTLEIGGE
                     VENPFSLNHQQLLNTFPLEERIYRFRCVEAWSMVVPWIGFELARLIEMAKPTSKAKYV
                     VFHTLYDPAQMPGQKNRLLGGGIDYPYVEALRLDEALNSLTLLSVGLYGKTLPPQNGA
                     PIRLVVPWKYGFKSIKSIVKITFAEKRPLTTWESLAPNEYGFYANVNPNVDHPRWSQA
                     SERVIGAGGLLSVKRQPTLMFNGYENEVANLYRGLDLKVNY"
     gene            1035..1619
                     /locus_tag="PXA33_002554"
     CDS             1035..1619
                     /locus_tag="PXA33_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoxide reductase heme-binding subunit YedZ"
                     /protein_id="HDL5121837.1"
                     /translation="MLTWLRILIHLGCLLPLVWVAQLLYTGNETILGADPIKELEHFL
                     GYGAIVIFCIMFLLGIALQYLKKNQYQILRRPLGLWAFVWAALHISSYLLLELSLDVK
                     LFFSELAGRPYLILGAAAFFILSIMAITSLPMLKRKLGTRWGTIHQWAYPALVLAMVH
                     YYWSVKSVTFDPIIIGVLVLFIVAHKIQAKFAKK"
BASE COUNT          532 a          325 c          336 g          498 t
ORIGIN      
        1 aaaattacac ttattttgta agaattctag atcagctgag tccaaaataa taacaacaat
       61 tcataaaaaa aggatagttc gatgaaaaaa ttaacaattc aagatgttac acccgaaact
      121 attttctacc aacgccgtaa aattattacc gcgttaggtt taggtattgc agcttctgca
      181 ttgcctaaag taagtatggc agaaacacaa agacacttgc aatcactaca gtttaaaccg
      241 gatccaaata aagggcttga actcactccg gaaaacaaag taataggcta taacaatttc
      301 tatgagtttg gcgtggataa aagtgcacca gcaaaaaatg cggcaaactt caaaaccgat
      361 ccttggacat tggaaattgg cggagaagtt gaaaatccgt ttagcttaaa tcatcaacaa
      421 ttgcttaata cgtttccact agaagaacgc atttaccgtt ttcgctgtgt tgaagcgtgg
      481 tcaatggttg tgccgtggat cggctttgag ttagctcgct taattgagat ggcaaaacca
      541 accagtaaag ccaaatatgt ggtctttcac actttatatg atcctgcaca aatgccgggg
      601 caaaaaaatc gcttgctagg cggtgggatt gattatcctt atgtagaagc tttacgctta
      661 gatgaagcct taaattcatt aacactacta tccgtcggtt tatacggtaa aaccctgcca
      721 ccgcaaaacg gtgctcctat tcgcttagtc gtgccttgga aatatggctt taaaagtatt
      781 aagtcgattg tgaaaatcac ctttgctgaa aaacgcccac tcactacttg ggaaagtctt
      841 gctccaaatg aatatggctt ttatgctaat gtgaatccaa atgttgatca tccacgttgg
      901 tcgcaagcat ctgaacgagt aattggtgca ggcgggttat tatccgtcaa acgccaacca
      961 acactaatgt ttaatggcta cgaaaatgaa gtggcaaatt tataccgtgg cttagattta
     1021 aaggtgaact actaatgcta acctggttac gtatactcat tcatcttggc tgtttattac
     1081 cgctagtttg ggtagcacag ctattatata cgggtaatga aacaatatta ggtgccgatc
     1141 cgattaaaga gctggagcat tttctcggtt atggtgcaat cgtcattttt tgtataatgt
     1201 ttctacttgg tattgcccta caatacctaa agaaaaacca gtatcaaatt ttacgccgtc
     1261 cattgggctt atgggcgttt gtatgggcag ctctacatat ttcaagctat ctattgttag
     1321 aattaagcct tgatgtaaaa ttatttttta gcgagcttgc cggtcgccca tatttgattt
     1381 taggagcagc tgcttttttc attctaagca taatggcaat aacttcgctg cctatgctaa
     1441 aacgaaaatt aggaacacgc tgggggacga ttcaccagtg ggcttatcct gcattagtat
     1501 tggcgatggt acattattat tggtcggtaa agagcgtaac attcgatcca attatcatcg
     1561 gagtcttagt gctgtttatt gttgctcata aaatacaggc taaatttgca aaaaaatagc
     1621 aaaatcacac cgcttgcagc agtcaaacta catacaaaaa ataaggggct gtcctagata
     1681 acgactaaaa c
//
LOCUS       DANLRK010000264         1675 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.265, whole genome shotgun sequence.
ACCESSION   DANLRK010000264 DANLRK010000000
VERSION     DANLRK010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.265"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            <1..700
                     /locus_tag="PXA33_002555"
     CDS             <1..700
                     /locus_tag="PXA33_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121838.1"
                     /translation="ILNGALIKEPRNPLIYHRHSWHLEFDGSVLYIADPTLFKYESIN
                     LAWYVGDKDTDLYPYLAEFVTSVATHLNVETDHIVTYGSSGGGFAALQLGARIGNGVV
                     TVCINPQTNIMKYVPSHRNAFLNACFDFNAEEYDSLSNQEKFNALVLLKENTSKILYI
                     QNTQDEFHHKWHFLPFLELKGIDLSEIEKTHWREQQSKDVKVILYSHESGHAAEPKEM
                     IPEILSAVDQLIAS"
     gene            702..>1675
                     /locus_tag="PXA33_002556"
     CDS             702..>1675
                     /locus_tag="PXA33_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015586968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL5121839.1"
                     /translation="MLFYLVSDSLTRLSLSNESLVDLGRITSIGNYPFLHNAKNAKFL
                     MVESAWQGYKNRWKYKIASYPDNPERTNEKLVRLVEKAKDKGIPTVFWNKEDSVHFDR
                     FIDSAKHFDHIFTVDQNAIARYREVVPATTTVNVGMFPVQPKIHNFQGFNFKHLAANF
                     VGSYSHHIHNKRRERQDMLFGAALKAGLPVTIFDRNSDRKSENYRYPKNSFNLNVQPN
                     VSYTETANIYRDYLVSLNINTVEDSPTMFSRRVVEILACGGILVSTPSMAIDNIFKEY
                     CHIVQSEDEAIELFQRLKRGASRDDLNKAIAGSEFVLSNFTWEHFLSQ"
BASE COUNT          548 a          273 c          311 g          543 t
ORIGIN      
        1 gatattaaat ggtgcattaa tcaaagaacc cagaaatccc ttaatttatc atagacatag
       61 ttggcatcta gaatttgatg gctcagttct atatatcgca gatccaacat tatttaaata
      121 tgaatcaatt aatttggctt ggtatgttgg agataaagat actgatttat atccatactt
      181 agctgaattt gtaacttctg ttgctacaca tttgaatgtt gagacagacc atattgttac
      241 ttatggttca agtggtggtg gatttgctgc attacagttg ggagcaagaa ttggaaacgg
      301 tgtagttacc gtttgtatta atccacaaac caacatcatg aaatatgtac caagtcatcg
      361 taatgccttt ttaaacgctt gctttgactt taatgcggaa gagtacgata gtctttctaa
      421 tcaagagaaa tttaatgctt tagtattact taaggaaaat acatcaaaga ttttgtatat
      481 tcaaaatact caggatgaat ttcatcataa atggcatttc ctcccattcc tagagttaaa
      541 ggggatagat ctatcggaaa tagaaaagac ccattggaga gaacaacaga gcaaagatgt
      601 aaaagttatt ttgtattctc atgaatcagg tcatgcagct gaacctaagg aaatgattcc
      661 cgagatatta tccgctgttg atcaactgat agctagctaa aatgctgttt tatttggtat
      721 ctgattcttt aactaggctt tcattatcaa atgaaagcct agttgatctt ggaagaataa
      781 catctatagg aaattatcct ttcttgcata atgcaaagaa tgctaaattt cttatggtcg
      841 aatccgcctg gcaaggctac aaaaaccgtt ggaaatataa aattgcctct tatcctgata
      901 atcctgaacg aaccaatgaa aagttggttc gtttagtaga aaaagcgaag gataaaggca
      961 ttccaacggt cttttggaac aaagaagatt ctgtgcattt tgatcgattt attgattcgg
     1021 cgaagcattt tgatcatatt tttaccgtag atcaaaatgc cattgctcgt tatagggaag
     1081 tagtgcctgc gacaacaacc gtcaatgtcg gaatgttccc tgttcagccc aaaatccata
     1141 actttcaagg atttaatttt aaacaccttg ccgcaaattt tgtagggagt tacagtcatc
     1201 atatccacaa taagcgtaga gaaagacagg atatgttgtt tggtgcagca cttaaagcag
     1261 gtcttccggt aaccatcttt gatagaaact ctgatagaaa atcggaaaat tatcgttacc
     1321 caaaaaatag ctttaattta aatgttcaac ctaatgttag ttatactgaa actgctaata
     1381 tttatcgtga ttatcttgtt tctttgaata ttaatactgt cgaagattcc cctacgatgt
     1441 tttctcgcag agtcgttgaa atattagctt gtggaggaat attagtctct acaccaagta
     1501 tggctatcga caatatattt aaagaatatt gtcatatcgt acaaagtgaa gatgaggcta
     1561 tagaattatt ccaacgttta aaacgaggtg cttctcgtga tgatttgaat aaagcgattg
     1621 caggctctga atttgtattg agtaacttta cttgggaaca tttcttatcc caaat
//
LOCUS       DANLRK010000265         1666 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.266, whole genome shotgun sequence.
ACCESSION   DANLRK010000265 DANLRK010000000
VERSION     DANLRK010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.266"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            32..751
                     /locus_tag="PXA33_002557"
                     /pseudo
     CDS             32..751
                     /locus_tag="PXA33_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616566.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            811..1260
                     /locus_tag="PXA33_002558"
     CDS             811..1260
                     /locus_tag="PXA33_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudoazurin"
                     /protein_id="HDL5121840.1"
                     /translation="MLAKLKWNQLACVTLAFCSASVFSAHHEIKMLDNNAEGSMVFEP
                     GYVKAEVGDTVTFIPSHKGHWVESRSIPEGAEKFLSKEDEEFTITLTHEGVYVYYCPP
                     HRTMNMAGIIQVGQALNLEATQKEVEKIEKRTKENKGRLFEYLEQVK"
     gene            1361..>1666
                     /locus_tag="PXA33_002559"
     CDS             1361..>1666
                     /locus_tag="PXA33_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Type II secretory pathway, pseudopilin PulG"
                     /protein_id="HDL5121841.1"
                     /translation="MYKAITLIEILTTITIMVIAVYFISPVIFTLQDRIALHSEIENI
                     QSFIQQIQTKARYTKRNYTLTLSQRNNQWCMIAVIKPINTSKQIICDCLNKHFCDLGK
                     "
BASE COUNT          551 a          290 c          342 g          483 t
ORIGIN      
        1 gttttagtcg ttatctagga cagcccctac ttttaatact cgtaagcccc cttttgataa
       61 ccctaaagtt cgtcaagcat tgaattatgc ggtgaataaa actgaaattt taacttccgt
      121 ttaccaaggc tctgcaacgc cagctaaaaa cccaatgcct tctacgattt ggggatataa
      181 caacgaagta gccggttatg aatttaatcc ggaaaaggcg aagagcttgc ttaaagaggc
      241 cggattagaa aatggctttg aaacagaaat ttgggcaatg cctgtttctc gcccttacaa
      301 tccaaatgcc cgtagaatgg ctgaaatgat tcaagaggat tggaaagcaa taggggtaga
      361 ggcaaagata gtgagctatg aatggggaga atatcttaaa cgaatgggaa acggcgagca
      421 cgctaccgga atgattggtt ggaccggaga taacggcgat ccagataatt tcctcaatgc
      481 cctactcagt tgtaatgcgg tagaacaagg ctcaaactat gcagggtttt gtaatccaga
      541 gtttgaccgt ttaattaatg aggctgcgac tatcagtgat caaaacaagc ggtcagattt
      601 atataaaaaa gcgcaattca tctttaatga gcaagcacct tggttaccga ttgcacattc
      661 aacggtgtat tttccggtaa ggaaagaggt aaaaggctat gttgtcagcc cgtttattgc
      721 tcataatttt tatggtgttg agctggaata acctaaggta taatgaattg aactcatttt
      781 gtgcctcata aatttaggag caataaatat atgttagcga aacttaagtg gaatcaatta
      841 gcttgtgtaa cattggcatt ttgttcagcc tcagtatttt cagcccatca cgaaatcaaa
      901 atgttagata acaatgcgga aggatcaatg gtatttgagc ccggttatgt aaaagcagag
      961 gtgggcgata cggtaacatt tattccgtct cataaagggc attgggttga aagccgctcg
     1021 attccggaag gtgcggagaa gttcttatct aaagaagatg aggaatttac tattaccctt
     1081 actcacgaag gtgtttatgt ttactactgt ccgccacacc gaaccatgaa tatggccgga
     1141 attatccaag taggacaagc tctcaatctt gaagccacac aaaaagaagt ggaaaaaatt
     1201 gaaaaacgca cgaaagaaaa taaaggacgt ttatttgaat atttagagca agttaagtag
     1261 aagtgattta attttgcgat ctgcatcgaa aaatcaatat ttaatagttt acaaatgatg
     1321 cttaaattct aagataccct ctatcaatga gggtattttt atgtataaag ccatcacatt
     1381 aattgaaata ctaaccacta tcactattat ggtgatcgct gtttatttta tctcgccggt
     1441 tatttttaca ctgcaagatc gaattgcact tcatagtgaa attgaaaata tccaatcttt
     1501 tattcagcaa atccaaacta aagcacgtta taccaaacgt aattatacct taaccctttc
     1561 tcagcggaat aatcagtggt gtatgattgc ggtaattaag ccaatcaata ctagcaaaca
     1621 aattatttgt gattgtttga ataaacattt ttgtgattta ggaaaa
//
LOCUS       DANLRK010000266         1621 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.267, whole genome shotgun sequence.
ACCESSION   DANLRK010000266 DANLRK010000000
VERSION     DANLRK010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.267"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(2..184)
                     /locus_tag="PXA33_002560"
     CDS             complement(2..184)
                     /locus_tag="PXA33_002560"
                     /inference="COORDINATES: protein motif:HMM:NF015431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL5121842.1"
                     /translation="MALLADSTVDRLVAENGLVEVLGETENPKFDLTMIYAAHRYPSS
                     IVRAFVGFVLEQVKAN"
     gene            complement(153..863)
                     /locus_tag="PXA33_002561"
     CDS             complement(153..863)
                     /locus_tag="PXA33_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL5121843.1"
                     /translation="MNLNTLRLFVAVIQHGSLSKASERLNVPIATISRQIADLEKELN
                     IQLFDRQKSGVKPTMAGQRLYEQVHLSIEQLATAKEVLFEEQGNLKGKLRLSASPACE
                     PVLAWIADFQAAYPQVQVHCTLTDRLLDLSADGIDVAFRIGQLHGEQFIAKKVGQIGS
                     KWVAHPKFLARFGTPSTVKDLKNFPIAAWAKNEASVITLGTGRQAVEIPYLFSSNDSY
                     AIEYMAKSSGSACRQHGG"
     gene            complement(860..>1621)
                     /locus_tag="PXA33_002562"
     CDS             complement(860..>1621)
                     /locus_tag="PXA33_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121844.1"
                     /translation="DDWVKINDFPDVSNLLEVTINDEQWLVLERHDDFRELAKFGQKE
                     TYDRQMWLQIRSYLVKESQFSKAKNWLAQQDFMERWMPEGNEHYGMNIHQFFAENTLN
                     EWQEIYRHRENKSPSFKVIPSVDSHTWESTYGNDGISFYAPIPMLYHGMKMKNAIKDG
                     YWVDLQNNLVCFNPEINEKFQNSLLVKKDKFLDFLKEHKLKLVWTVLGEKHAYKESKF
                     LQELSGVYYLYGKNQQVQGSIKHFIQEIEHWGEKQ"
BASE COUNT          452 a          361 c          289 g          519 t
ORIGIN      
        1 ctcaattagc ttttacttgt tccaacacaa accctacaaa cgcccgcaca atgctagacg
       61 gataacgatg ggcggcataa atcatcgtca aatcaaattt ggggttttct gtctcaccca
      121 agacttccac caagccattt tctgccacca atctatccac cgtgctgtcg gcaagcagag
      181 ccactgcttt ttgccatata ttcaatggca tagctgtcat tgctggaaaa aagataggga
      241 atttctaccg cttgtctgcc agtgcctagc gtgatgacgc ttgcttcatt ttttgcccaa
      301 gcggcaatgg ggaaattttt caaatctttg acggtgctgg gcgtgccaaa gcgtgccaaa
      361 aatttagggt gtgccaccca tttactgccg atttgcccca cttttttggc gataaactgt
      421 tcgccgtgta gttgcccaat ccgaaacgcc acatcaatgc catctgccga caaatccaaa
      481 agacggtcgg tcagcgtgca atgcacttgt acttgtggat aagctgcctg aaaatcagct
      541 atccacgcca aaacaggctc gcaggcaggg cttgccgaca ggcgtaattt accctttaaa
      601 ttgccttgct cttcaaacaa cacttctttg gcagtggcaa gctgttcaat ggacaaatgc
      661 acctgttcat acaggcgttg ccctgccatt gtgggtttaa cgcctgattt ttggcggtca
      721 aagagttgaa tgttaagctc tttttccaaa tcggcaattt gacggctaat ggttgcaatc
      781 ggcacattga gtcgctcgga ggctttggag agactgccgt gctggataac ggcgacaaag
      841 aggcggaggg tgttgaggtt cattgttttt ctccccaatg ttcaatttct tgaataaaat
      901 gtttaatact gccttggact tgttgatttt taccatatag ataataaacg cctgaaagtt
      961 cttgtaaaaa tttactttct ttataagcgt gtttttcacc caacacagtc caaactaact
     1021 ttaacttatg ctcttttaaa aaatccaaaa atttgtcttt tttaaccagt aagctatttt
     1081 gaaatttttc atttatctct ggattaaagc aaaccaaatt attttgtaaa tcaacccaat
     1141 aaccatcttt aatcgcattt ttcattttca ttccgtgata aagcatagga attggcgcat
     1201 aaaatgaaat gccgtcattg ccataagtag attcccaagt gtgagaatcg acactaggta
     1261 ttaccttaaa tgaaggcgat ttattttccc tatgtcgata aatttcttgc cactcattta
     1321 atgtattttc ggcaaaaaat tgatgaatat tcattccata gtgttcatta ccttctggca
     1381 tccatctttc cataaaatct tgttgtgcca accaattttt tgcttttgaa aattgagatt
     1441 ctttaactaa ataacttcta atttgtaacc acatttgcct atcataagtt tctttttgcc
     1501 caaattttgc taattcacga aaatcatcgt gtctttctag aacaagccac tgttcatcat
     1561 taatcgttac ttccaaaaga ttactaacat caggaaaatc atttattttt acccaatcat
     1621 c
//
LOCUS       DANLRK010000267         1482 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.268, whole genome shotgun sequence.
ACCESSION   DANLRK010000267 DANLRK010000000
VERSION     DANLRK010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.268"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            13..336
                     /locus_tag="PXA33_002563"
     CDS             13..336
                     /locus_tag="PXA33_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL5121845.1"
                     /translation="MKLSSELQNQINQLNQLRKQALKQEQDEQKLFEKNKIEDLPHFG
                     TQLNAQRKLLGIELTTLEMQTGISSSTLKRLFKDPSQVKFQTICIVAETLGFNLCAIN
                     THKQE"
     gene            complement(413..1431)
                     /gene="glpX"
                     /locus_tag="PXA33_002564"
                     /pseudo
     CDS             complement(413..1431)
                     /gene="glpX"
                     /locus_tag="PXA33_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484632.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
BASE COUNT          461 a          350 c          266 g          405 t
ORIGIN      
        1 ctcaaaaata aaatgaaact atcaagcgaa ctacaaaatc agataaacca actaaatcag
       61 ttacgaaaac aggcactcaa acaagagcaa gacgagcaga aattgtttga aaaaaataaa
      121 attgaagatt tgccacattt tggcacgcag cttaatgctc agaggaaact attaggcata
      181 gaattaacca cccttgaaat gcaaaccggc atttcaagct ctaccctaaa acgcttattt
      241 aaagacccaa gccaagtgaa atttcagaca atttgtattg tggcagaaac actcggcttt
      301 aatttatgtg caattaatac tcacaaacaa gagtaagcgt gacaatgtag tgattttgat
      361 tactttggga agacaaaaaa tcgggctaaa agcccgattt atcagagaaa agctataaat
      421 ccaataaaga aaggagttcg ttatctttac gatcgacata atggtcggat tgaattttac
      481 gaatggttct agatttgcca cgaataagta aggtttcagt gctggcaata ttgcctctac
      541 gtttaatccc ttttaacaat tcacctgaag taatgccggt tgcaacaaag acgatattgt
      601 catcacgcac caaatcttct aatttcaaca ccgtattcac cggtacattc atttcattac
      661 agcgtaaaag ctctttttcg gtagcagtta aattttccgg gctattcccc ttcacttggt
      721 tgcgaggaat taaacgtgct tgcatatcgc cacccaatgc acgaaccgca gcccccgcca
      781 ctaccccttc aggtgctccg ccgataccat aaagcagatc caattcgctc tctggcaaac
      841 agcattgcac cgcagccgca acatcgccat caggaatcgc aatcactctt acccctaatt
      901 tttgcacatc ggcaattatt ttttcgtggc gaggcttagc aagatagcaa tggttaattg
      961 ggaaagtaat ttgccctttt tcgaggcgac acggcgaaga ttttgttcaa gcggaagatt
     1021 taaatcaatc attcctttgc attcctgccc caccactaat ttttccatat acatatcagg
     1081 ggcttttaaa aaggtttctt tgccgcctgc tgctaatacc gacaacgcat ttgcctgccc
     1141 cattgcagtc atacgagtac cgtcaatcgg gtcaaccgca atactgattt cctcatcttc
     1201 taggcgggat agaccgattt tctcgccaat ataaagcatc ggtgcttcgt caatttcgcc
     1261 ctcaccaatt accacttcgc cacggatttc catttggttc agcataaacc gcattgcttt
     1321 caccgcagct tcatcagcgg catttttatc cccacggcct agccaagagt aagctgctag
     1381 agccgctgct tccgtaacac gagaaaattc aaaagaaaga gttcgtttca tttttgttcc
     1441 ttagtttctt gaaagaaaaa ctttaaaaga agagagatta ta
//
LOCUS       DANLRK010000268         1458 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.269, whole genome shotgun sequence.
ACCESSION   DANLRK010000268 DANLRK010000000
VERSION     DANLRK010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1458
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.269"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            <1..415
                     /locus_tag="PXA33_002565"
     CDS             <1..415
                     /locus_tag="PXA33_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121846.1"
                     /translation="GAREAVTDSRQSIFSSFWVKVWIILLPLALFYCGYNLYIFLNGS
                     ESEVSHNTEQAKFASDENHNDIVIHLLPTIGKPLSTKWRITGELKRDNKSFVILADNE
                     NRLRLEPSSQFSFSGRMLQGEIDGGIVSYFSGESK"
     gene            412..>1458
                     /locus_tag="PXA33_002566"
     CDS             412..>1458
                     /locus_tag="PXA33_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspD"
                     /protein_id="HDL5121847.1"
                     /translation="MKIHQLGLLLMISSFSYAKNVDFKLEAVPLPKAISIIYDEVLQK
                     PYMLDPKLSNDSRVISFHTTEQQNFEQFIQRYFSNMNISVSEKKGVVYIGHIEPKQPK
                     VIKRSFVYSPIHRDVDYLASFLKDGTEGNQVFASGDKLVYYGTSEDIARVKSILKSID
                     TKSREVVVTGYVFEVQNIEKEGSGINLLAKLLSGKLGISVGYKQSYENFITVNAGNLD
                     AMFELFRTDQRFRVVSSPTLRVKSGSRGNFSVGSDVPVLSNVTYQDGRPIQSIEYRSS
                     GVIFDIQPTIKDKAIDLKIQQLSNFIKTDTGVNNSPTLVKRDLVTDVTVKSGDVIVLG
                     GLAENKISNGETSFS"
BASE COUNT          490 a          209 c          306 g          453 t
ORIGIN      
        1 tggtgcaaga gaagcggtta cagatagtag gcaatctata ttttcatctt tttgggtcaa
       61 ggtatggatt attcttttac cattggcatt attttattgt ggctataacc tttacatttt
      121 cctaaatggt tcagagagtg aagtaagcca taatactgaa caggctaaat ttgcttctga
      181 tgaaaaccat aacgatatag taattcattt acttcctact attggaaaac ctttatcaac
      241 taaatggcga ataacagggg aactaaaaag ggataataaa tcatttgtga tcttagctga
      301 caatgaaaac cgtttaagac ttgagcctag tagccagttt agttttagcg gtcgaatgtt
      361 gcaaggagag atagatggtg gaatagttag ttatttttcg ggagaatcta aatgaaaatc
      421 catcaattag ggctattgct catgattagc tcattttctt atgctaagaa tgttgatttt
      481 aaattagaag ctgtgccgtt accgaaagca atatcaataa tatacgatga agtgctacaa
      541 aagccatata tgcttgatcc taaattatct aatgattcaa gggttattag ctttcataca
      601 acagagcaac agaattttga gcagtttatt caacggtatt tcagcaatat gaatatcagt
      661 gtatcagaga aaaaaggtgt tgtttatatt ggacatattg aaccaaaaca gccgaaagta
      721 ataaaacgta gttttgtgta ttcgcctatt catcgtgatg ttgattattt ggctagcttc
      781 ctgaaagatg gaacagaagg aaaccaagtt tttgcaagtg gcgacaagct tgtatattat
      841 ggtacttctg aagatattgc acgagtgaaa agtatcttga aatcaatcga cacaaaaagc
      901 cgtgaagtag tggtaactgg ttatgttttt gaagtgcaga atattgaaaa agagggtagt
      961 ggcataaact tgttagcgaa attgctatca ggaaaattag gcattagtgt tggttacaag
     1021 caaagttatg aaaactttat caccgtgaat gcaggaaatt tagatgcaat gtttgaattg
     1081 tttcgcacag atcagcgttt tagggttgtc agcagtccga cattaagagt aaaatcaggc
     1141 tcaagaggta atttttcagt aggctctgat gtccctgtat tatcgaatgt aacctatcag
     1201 gacggaaggc ctattcagtc gattgaatac cgttcaagcg gtgtaatatt tgatattcag
     1261 ccaacgataa aagataaagc gattgattta aaaattcaac aactatcaaa tttcatcaaa
     1321 acagataccg gtgtaaataa tagcccaacg ttggtaaagc gtgatctggt aacagatgtt
     1381 acggttaaaa gcggtgatgt tatcgtttta ggtgggttag ctgaaaataa aatatctaat
     1441 ggcgaaacca gtttttca
//
LOCUS       DANLRK010000269         1389 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.270, whole genome shotgun sequence.
ACCESSION   DANLRK010000269 DANLRK010000000
VERSION     DANLRK010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.270"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            107..1369
                     /locus_tag="PXA33_002567"
     CDS             107..1369
                     /locus_tag="PXA33_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HDL5121848.1"
                     /translation="MLKRDMNIADYDAVLWQAIQDEDRRQEEHIELIASENYASPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKALFGADYANVQPHSGSQAN
                     AAVYMALLNPGDTILGMSLAHGGHLTHGASVSFSGKIYKAEQYGITDEGLIDYEALRK
                     QAQEVKPKMIVAGFSAYSQVMDWAKMREIADEVGAYLFVDMAHVAGLIAAGVYPNPLP
                     HAHVVTTTTHKTLAGPRGGLILSASGDEEMYKKLQSAVFPAGQGGPLMHVIAAKAVCF
                     KEALEPEFKAYQQQVVKNAKAMVEVFKQRGFDVVSNGTENHLFLVSFVKQGLTGKAAD
                     AALGAANITVNKNSVPNDPQKPFVTSGIRVGTPSVTRRGFKEAEVTALAGWMCDVLDS
                     IGKDNHEQVIAETKAKVLDICKRLPVYA"
BASE COUNT          425 a          257 c          323 g          384 t
ORIGIN      
        1 agttaatttt tatataaata atactatcta tgaaactaaa aaatgcgtaa aataacgtga
       61 aatcaactta cgcaaacgtt tacttaagct tattaggagt ccaaaaatgt taaaacgtga
      121 tatgaatatc gctgattacg atgcagtatt atggcaagcc attcaagatg aagatcgccg
      181 tcaagaagaa catattgaat taattgcatc tgaaaactat gcaagcccac gtgtaatgca
      241 ggctcaaggc tcacaattaa ccaataaata tgcagaaggt tatccgggta aacgttacta
      301 tgggggttgt gagtatgtgg atattgtaga acaacttgcg attgaccgtg cgaaagcatt
      361 gttcggggca gattatgcca acgttcaacc gcattcaggc tcacaagcga atgcagccgt
      421 atatatggcg ttgcttaatc cgggtgacac tattttaggt atgagtttag cgcacggcgg
      481 gcatttaacc cacggtgctt cagttagctt ctctggtaaa atttataaag cagaacaata
      541 cggcattact gatgaaggct taattgatta tgaagcatta cgcaaacaag cccaagaagt
      601 gaaaccgaaa atgattgttg ctggtttctc tgcttattct caagttatgg attgggcaaa
      661 aatgcgtgag attgccgatg aagttggggc atatttattt gtggatatgg ctcacgttgc
      721 aggcttaatt gcggcgggag tttatccaaa tccgcttcct cacgctcacg tcgtgacgac
      781 aacaacccat aaaaccttag ccggccctcg aggtggtttg attttatctg catctggcga
      841 tgaagaaatg tataagaaac tgcaatctgc cgtattccct gctggtcaag gtggaccatt
      901 gatgcacgtt attgcggcaa aagcggtttg ttttaaagaa gctttagagc cggaatttaa
      961 ggcatatcag caacaagttg taaaaaatgc aaaagcaatg gttgaggtat ttaagcaacg
     1021 tggttttgat gtggtttcaa acggtacgga aaaccattta ttccttgtta gctttgtaaa
     1081 acaaggttta accggcaaag cggcagatgc tgcattagga gctgcgaaca ttaccgtgaa
     1141 taaaaactcg gtgccgaacg accctcaaaa accattcgtg acttcaggta ttcgtgtggg
     1201 tacgccttct gtgacccgcc gtggttttaa agaagcagag gtgacagcat tagccggctg
     1261 gatgtgcgat gtgctagata gcattggtaa ggataatcac gaacaggtga ttgcagagac
     1321 taaagcgaaa gttttagaca tttgtaaacg tttaccagtg tacgcataat ctcaacttta
     1381 cactttttc
//
LOCUS       DANLRK010000270         1366 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.271, whole genome shotgun sequence.
ACCESSION   DANLRK010000270 DANLRK010000000
VERSION     DANLRK010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1366
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.271"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            <1..1104
                     /locus_tag="PXA33_002568"
     CDS             <1..1104
                     /locus_tag="PXA33_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015483985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL5121849.1"
                     /translation="GHYLCTKVKGVRFFNNIAIGGRNVSAYGATSCSDIFIFGNREQG
                     MAVTTVDVEAGVQVEDVQTGECVVANNIFRHDIWMSGAKNAIINSNLCRRLRVTSGNP
                     TDTGSNNVEFSNNQAKSIAISKYGSFDNPSLFIDNINFINNILEPSEFNQDVYIDGTI
                     LKQCQFINNRCISKSKQYSVRLARNNPFLNLTFKSNKLSDNPAIISGKQGVIFCDDLS
                     LGVNKHRLRLRFSSPINFTVNQEWQKMPFDRVLNDINGHVDIVSGQFIARTGGTFHLK
                     GKITVTNSSEDVLNLYQLRLLINNEEKERLINQSVSPKNSKSNTNGMTTINFDTVVDL
                     KVGDIVDLQYKTTHELTLLSDSFLSQIYVTQEF"
     gene            1105..1347
                     /locus_tag="PXA33_002569"
     CDS             1105..1347
                     /locus_tag="PXA33_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121850.1"
                     /translation="MTKKHYCTGWRNAPANINSCCHQHDRDYGIKGTVSRAEADKRLR
                     HCLISQGNPIKAWVFWVVVRLFGWIYYKEKNINGKS"
BASE COUNT          452 a          215 c          287 g          412 t
ORIGIN      
        1 ggacattatc tctgcaccaa agttaaaggt gtgcgatttt ttaacaatat agcaattgga
       61 ggtcgcaatg tatcagctta cggtgcaaca tcttgctctg atattttcat ttttggtaat
      121 cgtgaacagg ggatggcagt gacgacagtc gatgtggaag cgggcgtaca agtggaagat
      181 gtacaaactg gcgaatgtgt ggtagcgaat aatattttca gacacgatat ttggatgtct
      241 ggtgctaaaa atgccattat caacagcaac ctctgccgtc gcttgcgtgt cacatcaggt
      301 aatccgactg acacaggcag caacaatgta gaattttcaa ataatcaagc aaaatctatt
      361 gcaatatcga aatatggttc tttcgataat ccttcattgt ttattgataa tatcaatttt
      421 attaataaca ttttggagcc cagtgaattt aatcaagatg tgtatattga cgggacaatt
      481 ttaaaacagt gccaatttat taataatcgt tgtattagca agtctaagca atattctgtc
      541 agacttgctc gcaataatcc gttcttaaat ttaacattta agagtaataa attatcggac
      601 aatcctgcga ttatatcagg taagcaaggt gttattttct gcgatgattt gagcttagga
      661 gtaaataagc acagattaag attgcgattt tcatcgccga ttaattttac tgttaatcaa
      721 gaatggcaaa aaatgccgtt tgacagagtg ttaaatgata tcaatggtca tgttgatatt
      781 gtcagtggtc aatttattgc aaggacaggc ggcacttttc atttaaaagg taaaataacg
      841 gttacaaatt catcggaaga tgtgctgaat ttatatcaac tcaggctgtt aataaataac
      901 gaagagaaag aaagactgat taatcaaagt gtttcaccta aaaacagcaa atcaaataca
      961 aatggtatga caacaatcaa ttttgatact gtagttgatt taaaagtagg tgacattgtt
     1021 gatttgcagt ataaaacaac gcatgagttg acattactgt cagactcatt tttatctcaa
     1081 atttatgtga ctcaggagtt ttaagtgact aaaaaacact actgcacagg ctggcgtaat
     1141 gcgccagcca atatcaatag ttgctgtcat cagcatgacc gagactacgg tatcaagggc
     1201 acagtcagcc gagccgaagc cgacaagcgg ttaagacatt gcttaatttc gcaagggaat
     1261 ccgattaagg cgtgggtgtt ttgggttgtg gtgaggttgt ttgggtggat ttattacaag
     1321 gagaaaaata tcaatggaaa gtcataaaac aggaaatatc attact
//
LOCUS       DANLRK010000271         1340 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.272, whole genome shotgun sequence.
ACCESSION   DANLRK010000271 DANLRK010000000
VERSION     DANLRK010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1340
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.272"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            30..656
                     /locus_tag="PXA33_002570"
     CDS             30..656
                     /locus_tag="PXA33_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HDL5121851.1"
                     /translation="MTKIKFYGNLKKFGSEFSLEVKDTAEAIRALCTQISGLREALRD
                     GVYKVRIGKQYLDPSALEKGLFYCLKKGQTIHFTPVIKGAKSGGVFNFVLGAALIGAA
                     FFTGGASIAAWGAGAKMMGMLGASMLLGGVSQMLTKMPKAPTMGNETEKESSTAFSNL
                     NNLVTQGKPVPLAYGLIRTGSLVISQGVETITIKENQPASNKKTGFRK"
     gene            705..1316
                     /locus_tag="PXA33_002571"
     CDS             705..1316
                     /locus_tag="PXA33_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL5121852.1"
                     /translation="MNLTKAESNLNAKITEEKKARVDADKVNADKLTDITSRVANAES
                     TITNFQSTKANKSEVASLAQSSLQAVWKADAKSAVDSLSIGARNLLIDSTYNQHINYN
                     TKMSSITRTVYRGNMLIRLGMLGGAGVAGIVQAPAAQVSNIRQGQEYTLSLNVQGTAG
                     IQKTGLNYVFLMRADGANQRLATIPVIASLLNRPKIALGKMPV"
BASE COUNT          435 a          267 c          305 g          333 t
ORIGIN      
        1 tattttggct ataattgtaa tggtgagtca tgactaaaat taaattttac ggcaatttaa
       61 aaaaatttgg tagtgaattt agcttagaag ttaaagatac tgccgaagct atccgagcct
      121 tatgtacaca gattagcgga ttgcgtgagg cattacgaga cggtgtttat aaagtccgta
      181 tcggcaaaca gtatttagac ccgtcagccc ttgaaaaagg gcttttttat tgtctgaaga
      241 aagggcaaac cattcatttt acccctgtta tcaaaggagc gaaaagcggt ggcgtgttta
      301 attttgtttt aggtgcagcc ttgatcggag ccgcattttt tactggcggt gcttctattg
      361 cggcttgggg agctggagca aaaatgatgg gtatgttagg ggcttcgatg ttactaggag
      421 gagtttccca aatgctcacc aaaatgccaa aagcccccac aatgggtaat gaaacagaaa
      481 aagaaagctc aacggctttt tcaaatttaa ataacttggt gactcaaggc aaacctgtgc
      541 cgttggctta tggcttaatt cggaccggct cgcttgtgat atcgcaggga gttgagacga
      601 tcaccattaa agaaaaccaa ccggcaagca acaagaaaac aggctttaga aaataaggga
      661 taattaccga ttcagcacaa gcacagcaac ttaatcagtt atctgtgaac ttgaccaaag
      721 cagagagcaa tttaaatgcc aaaatcacgg aagaaaaaaa agctcgtgtt gatgcagata
      781 aagtgaatgc agataaactc acagacataa caagccgtgt tgcaaatgcg gaatcaacta
      841 ttaccaattt ccaatcaacc aaagcgaata aaagtgaggt tgcgagcctt gcacaaagtt
      901 cgttgcaggc agtttggaaa gctgatgcaa aatctgccgt tgattcactt tctatcggtg
      961 caaggaactt actgatagac agtacttaca accaacacat caactacaat acaaaaatgt
     1021 catcaatcac acgcacagtc tatcgcggca atatgttgat acgactcgga atgcttggtg
     1081 gcgcaggtgt ggccggtatt gtacaagcac cagccgctca agtaagtaat atcagacagg
     1141 gtcaagagta cacacttagc ctgaatgtac agggtacagc cggcatacaa aaaacggggc
     1201 tcaactatgt attcttgatg cgtgctgatg gggctaatca acggttagca acaatacctg
     1261 ttatagccag cctattaaac agacctaaaa tagcccttgg caaaatgccg gtgtaaccgc
     1321 tatccacggt ggctcaatag
//
LOCUS       DANLRK010000272         1336 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.273, whole genome shotgun sequence.
ACCESSION   DANLRK010000272 DANLRK010000000
VERSION     DANLRK010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1336
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.273"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(<1..>1336)
                     /locus_tag="PXA33_002572"
     CDS             complement(<1..>1336)
                     /locus_tag="PXA33_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL5121853.1"
                     /translation="QDISRLNILLEQKNIEENRNLERMISYQLGYALINACKSFSGFI
                     SLPSELLKIRKLAKDKKQQKLLPSSPVANTPKQQTALVTSAPARSQSIDLTRGLTLLD
                     PISELCWADAFTGYPLVRKSYADHIAGSTCKFAFFESAWQANKGSWIYAFTSPGLKHN
                     NAQALLDAIQKLKDKSIPIIFWNKEDPMHYEMFKPIAEKVDYVFTTDQLVVDKYKKEL
                     GHSNIWALPFAAPIKVTNPIGRFSLDTETVCFAGTYYAQNHENRKKQMDMLLPALLAY
                     NGCIYDRASKDTSGKYAYPEQYTHLIREGVNFNEIVKLYKKFKVFLNVNTITQSTTMM
                     SRRVYELLASGTPVVSTPSKAITAQFPGIVITVNNEKEAELAVHKLLTDSYYWHKQSM
                     LGIREVMSKHTYENRWEFACSVINNEPIKEKTPSVRIIAVYHNYLDLAIYIDS"
BASE COUNT          417 a          238 c          219 g          462 t
ORIGIN      
        1 gagaatctat atagatagct aaatctagat aattatgata aaccgcgata attcgaacac
       61 taggtgtttt ttcctttatt ggttcattat tgattacact acacgcaaat tcccaacgat
      121 tttcataagt atgttttgac attacttcac gtatgcccag catggactgc ttatgccaat
      181 aatagctatc tgtcagtaat ttatggacag caagctcagc ttctttctca ttatttacag
      241 taataacaat gccagggaat tgtgccgtaa tagctttaga tggcgtagat acaacaggtg
      301 tgccgctagc tagaagttca tacacacgtc ttgacatcat tgtggttgat tgagtgatgg
      361 tattaacatt taaaaatact ttaaatttct tatacaactt aactatctca ttgaaattta
      421 ccccttctcg gataagatga gtgtattgtt caggataagc atatttgcca cttgtatctt
      481 tagatgctct atcataaata cagccgttgt aagccaataa tgcaggtaac aacatatcca
      541 tctgtttttt acgattttca tgattttgag cataatatgt tccagcaaaa caaactgttt
      601 ctgtatcaag agaaaatctg ccaattggat tggttacttt aattggggca gcaaaaggca
      661 aagcccaaat atttgaatgc ccaagttctt ttttatattt atctacaacc aattgatctg
      721 ttgtaaaaac ataatctact ttttctgcga ttggcttaaa catctcataa tgcattggat
      781 cttctttatt ccaaaaaatg ataggaatgc ttttatcttt tagtttctga attgcatcta
      841 acaatgcttg agcattatta tgttttaagc ctggtgaagt aaaagcatag atccatgacc
      901 ctttatttgc ctgccaagca ctttcaaaaa aagcaaattt acacgtgctg ccagcaatat
      961 gatcagcata agattttcta actaaaggat accctgtaaa ggcatctgcc cagcataatt
     1021 cagaaatagg atccagtagt gtcaatcctc ttgtcaagtc aatagattga gatcgggcag
     1081 gtgcacttgt aactaatgca gtctgttgct ttggtgtatt tgcaacaggg gaagatggca
     1141 ataatttctg ctgtttctta tccttagcta acttcctgat ttttaataat tctgaaggca
     1201 aggatataaa accactaaat gatttacaag cgtttattaa agcatatcct aactgataag
     1261 aaatcattct ttcaagattt ctattttctt caatgttctt ttgctctaac aaaatattta
     1321 atctggatat gtcttg
//
LOCUS       DANLRK010000273         1253 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.274, whole genome shotgun sequence.
ACCESSION   DANLRK010000273 DANLRK010000000
VERSION     DANLRK010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1253
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.274"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            7..1221
                     /locus_tag="PXA33_002573"
     CDS             7..1221
                     /locus_tag="PXA33_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003244520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HDL5121854.1"
                     /translation="MNPSPRLLMCPGPTDADPRVLRAMSAPILGQFDPEFTSLMNETM
                     EMERRLFQTKNEQTYVVNSTSRGGLETVLTAAICPGDKVLIPAFGRFGYLLNEILARS
                     GADITIIEREWGTVFEPEEIEAELKKGGYKAVAIIHGETSTSMMQPLEEIGKICKQYD
                     AMLIVDTVATLGGVDIRVDEWGIDACIGGTQKCISAPSGTALITYNKRIEEIIAKRKR
                     VEKGIRAESDVDGTLPPIPSNYLDLAQLQDYWSPRRLNHHTETTSAQYGVHEALRIIL
                     QEGLEARFARHILNDKALCAGLEAMGLKIFGDRKHKAPVVTAFHIPEGMSGPALRSDI
                     LNHFGIELATSFGPLDGKVIRIGNMGFSSQKRNVLLTLGALEAVLLSHKVKVPSGDAV
                     AAALAVYKEADK"
BASE COUNT          385 a          261 c          265 g          342 t
ORIGIN      
        1 agagaaatta atccctcccc tcgtttatta atgtgccctg gcccaacaga tgctgaccca
       61 cgtgttttgc gtgcaatgtc tgcacccatt ttagggcaat ttgaccctga gtttacctca
      121 ttaatgaatg aaacaatgga aatggaacgc cgtttattcc aaaccaaaaa tgagcaaact
      181 tatgtagtca actccacctc tcgtggtggc ttagaaactg ttctaaccgc agcgatttgc
      241 ccaggcgata aagtgttaat tcctgcattt ggtcgttttg gttatttatt aaatgaaatt
      301 ttagctcgta gcggtgcaga tattaccatt atcgaaagag agtggggaac cgtatttgaa
      361 ccagaagaaa ttgaagcaga gttgaaaaaa ggcggctata aagcagttgc tattattcac
      421 ggcgaaacct caacctctat gatgcaaccg ttagaagaga tcggcaaaat ctgtaaacaa
      481 tatgatgcaa tgttaattgt agataccgtt gcaaccttag gcggtgtaga tattcgtgtg
      541 gatgaatggg gcattgatgc ttgtatcggc ggtacacaaa aatgtatttc cgccccatca
      601 ggcacagctt taatcactta taacaaacgg attgaagaga ttattgcaaa acgtaaacgt
      661 gtggaaaaag gcattcgtgc agagtctgat gtagatggca cattaccgcc aattccaagt
      721 aactacttag acttggcaca gctacaagat tactggagcc cacgccgttt aaatcaccac
      781 acagaaacca cttctgcaca atatggtgta cacgaagcgt tacgcattat tttacaagaa
      841 ggcttagaag ctcgttttgc tcgccatatt ttaaacgaca aagcattatg tgcaggttta
      901 gaagcaatgg gtttgaaaat ttttggcgat cgcaaacata aagcccccgt tgtaacggca
      961 ttccacattc cagaaggtat gagtggccca gcattgcgta gtgatatttt aaaccacttc
     1021 ggtattgaat tagccacctc attcggcccc ttagatggaa aagtaatccg tatcggtaat
     1081 atgggtttct caagccaaaa acgtaacgta cttttaacct taggtgcgtt agaagccgta
     1141 cttttaagcc ataaagtaaa agtgccttca ggcgatgcgg ttgcagctgc cttggcagtc
     1201 tataaagagg ctgataaata accaccttcc taaatttaca atacaactca cta
//
LOCUS       DANLRK010000274         1237 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.275, whole genome shotgun sequence.
ACCESSION   DANLRK010000274 DANLRK010000000
VERSION     DANLRK010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1237
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.275"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            13..699
                     /locus_tag="PXA33_002574"
     CDS             13..699
                     /locus_tag="PXA33_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL5121855.1"
                     /translation="MDTLASRLKIVLDQKGLTQEKFAEQIGVSQPSVFKILNGQTRNP
                     TRIYEIANVLDVDVNWLKTGKGKAPDFAKIGKNPTAYEEESSMLRLEVLDVYASAGNG
                     SFVTGDLTAYTHAVEFENAYFAQVFQRANAKGLSIINVDGDSMEPTIGNGDLLFVDTT
                     KSAYQGDGVYVFSYGENLYVKRLQFAGDELLVISDNPLYKEWRITSENEHKFQIHGKV
                     EFMQGHIRRV"
     gene            703..1221
                     /locus_tag="PXA33_002575"
     CDS             703..1221
                     /locus_tag="PXA33_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121856.1"
                     /translation="MEHQNLTHLVYITSHKQIGVYSISNLNIDDLYFKGYCIQKNRVI
                     TLRADRIIKQFDDLALAENYAKNIPQDVFYLFDSLLNQQRKEKITPIYKIGLCFTGFK
                     QARKNELIQLAVDNDLRVVQNVTGAVDFLIFDKESKTVGPAKLAKAEKLGIKIIDDEE
                     FLYMLETGVVPD"
BASE COUNT          423 a          202 c          245 g          367 t
ORIGIN      
        1 aaggagttcc taatggatac tttggcaagt cgtttgaaaa tcgttttaga ccaaaaaggt
       61 ttaacacagg aaaaatttgc agaacaaata ggtgtttcac agccatctgt ttttaaaatc
      121 ctgaatggac aaactcgaaa tcccacaagg atttatgaaa ttgcaaacgt cctagatgtc
      181 gatgtaaatt ggctcaaaac cggcaaaggc aaagcaccag attttgcgaa aatcggaaaa
      241 aatccgaccg cttatgaaga ggaaagctca atgcttcgac ttgaggtgtt agatgtatat
      301 gcctctgccg gcaatggtag ttttgtcacg ggagacttaa ccgcttacac gcacgcagta
      361 gaatttgaaa atgcctattt tgcacaagtc tttcaacgtg caaatgccaa aggcttatca
      421 attattaatg tagatggcga ctcaatggag ccaactatcg gtaacggtga tttgctgttc
      481 gtagatacca ctaaatccgc ttatcaaggc gatggtgtct atgttttcag ctatggcgaa
      541 aacttatatg taaaacgctt gcagttcgcc ggtgatgaat tgttagtcat ttccgacaat
      601 ccgctttata aagagtggag aatcacatca gaaaatgagc ataaattcca aattcacggt
      661 aaagtggaat ttatgcaagg gcatattagg agggtttagg tgatggaaca tcaaaatcta
      721 actcatcttg tctatattac atcacataaa caaattggag tttattcgat ttctaatttg
      781 aatattgatg acctttactt caaaggttac tgtattcaga aaaatagagt tatcacatta
      841 agagctgatc gcattattaa acaatttgat gatttagctc ttgccgaaaa ttatgccaaa
      901 aatattcctc aagatgtgtt ttatctattt gattctcttc tgaaccagca acgaaaagaa
      961 aaaataacac cgatctataa aatcggtttg tgttttactg gatttaagca agctagaaaa
     1021 aatgaattaa ttcaactagc agtagataat gatttgagag tagtacaaaa cgtaactggt
     1081 gcagttgatt ttttaatttt cgataaagag tcgaaaactg ttggaccagc aaaactagca
     1141 aaagctgaaa agttaggaat taaaatcatc gatgatgaag aatttttata tatgttagaa
     1201 actggcgttg tgccggatta ggtttgactt ctcaata
//
LOCUS       DANLRK010000275         1228 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.276, whole genome shotgun sequence.
ACCESSION   DANLRK010000275 DANLRK010000000
VERSION     DANLRK010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1228
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.276"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            <1..692
                     /gene="purU"
                     /locus_tag="PXA33_002576"
     CDS             <1..692
                     /gene="purU"
                     /locus_tag="PXA33_002576"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005641775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HDL5121857.1"
                     /translation="TELNGIFNDETLLTDLKFTLPEGSSYKLLPKQRKRIVILVTKEA
                     HCLGDLLMKNYYGGLDVEIAAVIGNHETLKSLVERFDIPFHLVSHENLTRVEHDKLLA
                     EKIDEYSPDYIVLAKYMRVLNPEFVARYPNRVVNIHHSFLPAFIGAKPYHRAYERGVK
                     IIGATAHFVNDELDEGPIIMQNVINVDHTYTAEAMMRAGRDVEKTVLSQALELVLADK
                     VFVYKNKTIIL"
     gene            complement(729..>1228)
                     /locus_tag="PXA33_002577"
     CDS             complement(729..>1228)
                     /locus_tag="PXA33_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDL5121858.1"
                     /translation="YREYAVVLGTAKFYSKDVINQYYKYRLEAAKQLVQEGKVNQLLL
                     SGDNKTPYYNEPKTMTSDLLKMGIATSKIKQDYAGYTTFYSIIRAKKVYKLPPFTIIS
                     QKFHCERALFIAKFRDIDAICYAATYPEGAYQVRMREILARSAMVFSLLIGKEPESLE
                     DIRSK"
BASE COUNT          388 a          241 c          214 g          385 t
ORIGIN      
        1 gtaccgaatt aaacggaatt tttaatgatg aaaccttact cactgattta aaatttacct
       61 tacctgaagg ctcaagctat aaattactcc ctaaacaaag aaaacgtatc gttattttag
      121 ttaccaaaga agcacattgt ttaggtgact tactgatgaa aaattactat ggtggattag
      181 atgtagaaat tgctgccgtg attggaaacc acgaaacatt aaaaagttta gttgaacgtt
      241 tcgatattcc attccattta gtcagccacg aaaatttaac ccgagtggaa cacgataaat
      301 tattagcgga aaaaattgat gaatattcac ctgattatat tgtgttggct aaatatatgc
      361 gggtgctgaa tccggaattt gtcgctcgtt atccaaaccg agttgtcaat attcaccact
      421 cgttcttacc cgctttcatt ggtgcaaaac cttatcatcg tgcatacgaa cgtggagtga
      481 aaattatcgg tgcaaccgcc cattttgtga atgatgaatt agatgaaggc ccgattatta
      541 tgcaaaatgt gattaatgtt gatcacacct acaccgcaga ggctatgatg cgtgccggac
      601 gtgatgtcga gaaaaccgta cttagccaag ccttagaatt agtgcttgct gacaaagtat
      661 ttgtatataa aaacaaaacc attattttat aagcaaaata gccggtcaac attatctgac
      721 cggctatctc atttagaacg gatatcttcc aatgattcag gctctttacc tatcaacaaa
      781 ctaaaaacca ttgccgatct ggctaaaatt tcccgcattc gtacctgata agccccctct
      841 ggatatgtag cggcataaca aattgcatca atatccctaa attttgcaat aaacaatgcc
      901 ctttcacagt ggaatttttg ggagataatc gtaaatggcg gtaatttata tactttttta
      961 gccctaatta ttgaataaaa agtggtatac cccgcataat cctgcttaat ttttgaagtc
     1021 gcaatcccca ttttaagtag atcacttgtc attgtttttg gctcattata gtacggcgtt
     1081 ttattatctc cacttaacaa aagctgattg acctttcctt cttggactaa ttgcttagcg
     1141 gcttccaaac gatatttata atattggtta atcacatctt tagagtaaaa ttttgccgta
     1201 ccgagtacaa ctgcatactc ccggtaag
//
LOCUS       DANLRK010000276         1206 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.277, whole genome shotgun sequence.
ACCESSION   DANLRK010000276 DANLRK010000000
VERSION     DANLRK010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1206
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.277"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            <1..587
                     /locus_tag="PXA33_002578"
     CDS             <1..587
                     /locus_tag="PXA33_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL5121859.1"
                     /translation="AENAKFFEKMTACFGDLSQFEQKIRQIAEIAGIDLAAYEIDHLA
                     VRMNQTETAEQWKILLLDGATLLKESEVNGRPIGLFALIQAVEFCGQFVKIVELPFPK
                     NKVYLEEGWEHIELVFPMLDNESSEQWVSRALSHFRLADNSEIKLKISQPQVEGERLP
                     NPSIAISLRNVTFCNPYCLKLHPYDIKSVIMSEN"
     gene            608..1072
                     /locus_tag="PXA33_002579"
     CDS             608..1072
                     /locus_tag="PXA33_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HDL5121860.1"
                     /translation="MKKLALAVALMSSFALVGCANTDVFSGSVYSGEQAKEARSIAYG
                     TIVSVRDVKIQARSEGVIGTVGGGVLGGVAGNAIGGGTGQAIATAVGAVAGAVIGNQV
                     EQKASQVSSLEMVIRKDDGKEIVVVQKKEDGFVPGKRVRIVGSNSDLNVSLL"
     gene            1103..1195
                     /locus_tag="PXA33_002580"
                     /pseudo
     CDS             1103..1195
                     /locus_tag="PXA33_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
BASE COUNT          367 a          192 c          296 g          351 t
ORIGIN      
        1 tagcagaaaa tgcaaaattt ttcgagaaaa tgaccgcttg ttttggcgat ttatcgcaat
       61 ttgaacagaa aatccgacaa attgctgaaa tcgccggcat tgatttagca gcgtatgaaa
      121 tcgatcatct tgcggtaaga atgaaccaaa ccgaaaccgc agaacagtgg aagatccttt
      181 tgcttgatgg ggcaacctta cttaaagaga gcgaggtaaa cggcagaccg atagggctgt
      241 ttgcattaat acaagcggtt gaattttgcg gacaatttgt aaagattgtc gagttgcctt
      301 ttccgaaaaa taaggtttat ttagaagaag gctgggaaca tattgagctg gtttttccga
      361 tgttagacaa cgaaagttcg gaacaatggg tgtcaagggc attgtcgcac tttagacttg
      421 ctgataattc tgagattaaa ttaaaaatca gccaacctca ggtagaaggg gagagattgc
      481 caaatccaag cattgcgatc agcttgagaa atgtaacttt ttgtaatcct tattgtctaa
      541 agctccaccc ttatgatata aaaagtgtaa ttatgtcaga aaattaacgt ttcggagaac
      601 aaacaaaatg aaaaaattag ctttagctgt cgcattaatg tcaagttttg ctttagttgg
      661 ctgtgcaaat acagacgtgt ttagcggaag cgtttattcc ggtgagcagg caaaagaagc
      721 tcgttctatc gcttatggca ctattgtttc agtgcgtgat gtgaaaattc aagcacgcag
      781 tgaaggtgtg atcggtactg tcggcggtgg tgtattaggt ggcgttgccg gtaatgcgat
      841 tggcggtggt acagggcaag cgattgcaac cgcagtgggg gcagttgccg gtgcggtaat
      901 tggtaatcaa gtagaacaaa aagcgagtca agtttcttca ttggaaatgg ttattcgcaa
      961 agatgatggt aaagaaatcg tggtcgttca aaagaaagaa gacggttttg tgcctggcaa
     1021 acgtgtgaga atcgtagggt ctaattcaga cctgaatgtt tctctgcttt aattttagtt
     1081 tttcctagag gatatattat taatgaaaaa actttcttta gctgtattag cttcacttat
     1141 gttagcagct tgtactgcag atgtttattc tgataaaggt aatgcaacat ttatctagca
     1201 aagcag
//
LOCUS       DANLRK010000277         1200 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.278, whole genome shotgun sequence.
ACCESSION   DANLRK010000277 DANLRK010000000
VERSION     DANLRK010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1200
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.278"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            157..>1200
                     /locus_tag="PXA33_002581"
     CDS             157..>1200
                     /locus_tag="PXA33_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121861.1"
                     /translation="MKFILFDSILERHLGESLKRALESLGHQAVFTDLILHGHSMIYK
                     QADIDFMWKKVKDICDEKADMFISFRPMNLTTEMLEYIGKRMKTAIWLSDDPVLYKTC
                     YSQVVNHYDVLLHCGYKEVMDFYEANNHPKGFNFPFWTDHVAFPSVYNPLHSDYEIAF
                     LGNMNGQVRRKRYMELASLPFTKKVFGLIDSDPLAMHGGFINEAYLHTKRVTEVLSKA
                     KVGVSIPQFFTEYNGLDYDYPELAGLGYFQFPSRVIQYAASGLPIAAVGDERMKEVYP
                     EIFVGNSITELEPYIKQICEDYDFALSESAKILTRFRKNYSALSRAMMLIDLVENLDK
                     LQSQTTQERAILFT"
BASE COUNT          395 a          173 c          231 g          401 t
ORIGIN      
        1 tcaatcttac ggccaataaa attcaggcta aaaattcacc tatctttata gctgatccat
       61 ttaatgtgct atatatagat gataaaaagt tattgaaagg tagagattcc ttacttaaat
      121 tggtttataa gacttctcca tatttagggg tttaatatga agtttatttt atttgattct
      181 attttagaac gtcatttggg cgaaagttta aaaagagctt tggagtcgtt agggcatcaa
      241 gcagtattta cagatttaat tttgcacggc cattctatga tttataaaca agcggacata
      301 gatttcatgt ggaaaaaagt aaaagatatt tgtgatgaaa aagcagatat gtttatctca
      361 tttcggccga tgaatctaac gacagaaatg cttgagtata taggcaagcg aatgaagacc
      421 gctatttggc tttcagatga ccctgtttta tataaaactt gctattctca agtagtaaat
      481 cattatgatg tattattaca ttgcggatat aaagaagtta tggattttta tgaagctaat
      541 aaccatccaa aaggctttaa ttttccgttt tggacagatc atgtagcatt tccaagtgta
      601 tataacccgc ttcattctga ttatgagatt gcatttttag gtaatatgaa tgggcaagtt
      661 agacgcaaaa gatacatgga attggcttca ttaccattta ccaaaaaagt attcggttta
      721 atagattcag atccattagc aatgcatggt ggatttatca atgaagcata tttacataca
      781 aaacgtgtaa cagaggtttt atctaaggca aaagttgggg tgagtatccc acaattcttt
      841 accgaatata atggtttaga ttatgattat cctgaattgg cgggtttagg atatttccaa
      901 tttcctagtc gagttatcca atatgctgca agtgggttac ctattgcagc ggttggtgat
      961 gagagaatga aggaagttta tcctgaaatc tttgttggca atagtattac agagctggag
     1021 ccgtacatta aacaaatttg tgaagattat gattttgcac tatcagaatc ggctaaaatt
     1081 ctaactagat ttagaaagaa ctattctgcc ttatcccgtg ccatgatgtt gattgatttg
     1141 gtagaaaatt tagacaaact acagagccag acaacacaag agcgcgcaat tttatttact
//
LOCUS       DANLRK010000278         1139 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.279, whole genome shotgun sequence.
ACCESSION   DANLRK010000278 DANLRK010000000
VERSION     DANLRK010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1139
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.279"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            <1..188
                     /locus_tag="PXA33_002582"
     CDS             <1..188
                     /locus_tag="PXA33_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
                     /protein_id="HDL5121862.1"
                     /translation="AKPIADSNGLFILAQKKAKVWIYSYISPKTGKRSTKNRIGSYPQ
                     MSLVEQSPQVFEQIARL"
     gene            240..827
                     /locus_tag="PXA33_002583"
     CDS             240..827
                     /locus_tag="PXA33_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SocA family protein"
                     /protein_id="HDL5121863.1"
                     /translation="MFDELKIAQATAYLLHKAGGTMEHIKVMKLLYLADRLSWQRYGY
                     SISNDEYYSLPYGPVLSNTLNLMRGEEASLYQKTSIWGEWIADKEDHKISLKKSVNTE
                     DEYFFDCLSESDTEILEEVFATFGNYKPFALVEYTHNPKYIPEWEDPQGRSKPISLST
                     LLTKLGKTPSEIESILEQQAEKGTMDNLFKGLGNA"
     gene            820..1137
                     /locus_tag="PXA33_002584"
     CDS             820..1137
                     /locus_tag="PXA33_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121864.1"
                     /translation="MLEIKRQVTILRPTNYGKEENHYKHLYILLTDPCFDGETGKGDM
                     ILSVNCSSIKDGKALDETCILKAGTHEFITRDSFIFYRHIKIDSLDEIKANIEAGYFI
                     QKP"
BASE COUNT          410 a          215 c          236 g          278 t
ORIGIN      
        1 atgcgaagcc tatcgcagac agtaacgggc tatttattct tgcacagaaa aaagcgaaag
       61 tatggattta tagctatatc agccccaaaa caggcaagcg aagcacaaaa aatagaatcg
      121 gtagctatcc gcaaatgagc ttagtggaac aatccccaca ggtatttgaa caaatcgcac
      181 gtctttaaaa tattaaatta agagaaagca ctatttgcga tgtagcagga gataaaacaa
      241 tgtttgatga attaaaaata gcccaagcga ccgcctattt gctacataag gcaggcggaa
      301 caatggagca tattaaggta atgaaattgc tttatcttgc ggatcgctta tcgtggcaaa
      361 gatacggcta ttctatttct aatgatgaat attattcatt gccttatgga ccggtattaa
      421 gcaacacact caacctaatg cgaggagaag aagcgagctt ataccaaaaa accagtattt
      481 ggggcgagtg gatagcagat aaggaagatc acaaaatcag ccttaaaaaa tccgtaaata
      541 ccgaagatga atacttcttt gattgccttt cagagagcga caccgaaatt ttagaagagg
      601 tatttgcgac ctttggaaat tataagccat tcgccctagt ggaatatacc cataacccta
      661 aatacattcc cgaatgggaa gacccacaag gcagaagcaa gccgatttct ttaagcacgc
      721 ttcttactaa gctagggaaa acgccaagcg agatcgaaag cattttagag caacaggcgg
      781 aaaaagggac aatggataac cttttcaagg gcttaggcaa tgcttgaaat caaaagacaa
      841 gtaacgattc ttagaccgac caactacggc aaggaagaga accactacaa gcatttatat
      901 attctgctta ccgatccgtg ctttgatggt gaaaccggta aaggagatat gattttaagt
      961 gtaaactgct caagcattaa agacgggaaa gcacttgatg aaacttgtat tttaaaggca
     1021 ggaacacacg aatttatcac acgggacagc ttcattttct accgccatat aaaaattgat
     1081 tcgttagatg agattaaggc aaatatagaa gccggttatt ttattcaaaa gccctaatt
//
LOCUS       DANLRK010000279         1134 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.280, whole genome shotgun sequence.
ACCESSION   DANLRK010000279 DANLRK010000000
VERSION     DANLRK010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1134
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.280"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            <1..1047
                     /locus_tag="PXA33_002585"
     CDS             <1..1047
                     /locus_tag="PXA33_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121865.1"
                     /translation="LTFAATLVLSACGGSGSGGSSSTPNHPKPVLVPKTQNNLQAQNV
                     PQAQNASQAQNAPQAQNAPQAQNAPQVENAPQAQNAPQVENAPQAEVTPPVPQPQSQK
                     IDGSFDKIGSVKLNKEAQTLELSRFTLVDKLGTPPKFDKVSGKKIIEEKDFLVLNLSD
                     INAEQLSGDFLIRRSDDLFYGYYHDTNGKNLVDAADKFSQYFVVYDEKRVNDNISDKL
                     TATYRKKEGFVYGSNPHTKEFAARISKLGDVEIKFENGQAQGSIKDEKDGNAEIFTIK
                     GDTKQLEITPTESNRIIIAILDQNQKSYTPGMEKAIMETKFIDSKAGNSDQKYLIGEA
                     KSDNWQAIMVSEKK"
BASE COUNT          419 a          210 c          213 g          292 t
ORIGIN      
        1 ttaacatttg ctgcaacctt agttttaagt gcttgcggag gaagcggtag cggaggttcg
       61 tcttcaacac cgaatcaccc caaaccagta ctagtaccaa aaacacaaaa taatcttcaa
      121 gcacaaaatg ttcctcaggc acaaaatgcc tctcaggcac aaaatgcccc tcaggcacaa
      181 aatgctcctc aggcacaaaa tgctcctcag gtggaaaatg ctcctcaggc acaaaatgct
      241 cctcaggtag aaaatgctcc tcaagcagag gttactccgc ctgtaccaca gccacaatca
      301 caaaaaattg acggttcttt tgataaaatt ggttcagtaa aactcaataa agaggctcaa
      361 actcttgagc ttagtagatt cactttggtg gataaattag gcacaccacc gaagtttgat
      421 aaagtaagcg gtaaaaaaat tattgaagaa aaagattttc tcgtattaaa tttgtctgat
      481 attaatgctg aacaactctc tggcgatttt cttattcgcc gtagcgatga tctattctat
      541 ggctactatc acgatacaaa tggcaaaaat cttgtcgatg ctgccgataa attcagtcaa
      601 tattttgtcg tgtatgatga gaaacgggta aatgataata tctctgataa attaacagca
      661 acttaccgta aaaaagaagg ctttgtatat ggttcaaatc cacatactaa agaatttgcc
      721 gcacggatca gcaaattggg ggatgtagaa attaaatttg aaaatggtca agctcaagga
      781 agtataaaag acgaaaaaga tggaaatgct gagatcttta ctattaaagg tgatacaaaa
      841 cagttagaga ttaccccaac ggaaagtaac cgaatcatta tagcaatttt agaccaaaat
      901 caaaaaagct atactccagg aatggaaaaa gcaattatgg aaactaagtt tattgattca
      961 aaggctggta attccgacca aaaatactta atcggtgaag caaaaagcga taactggcaa
     1021 gcaataatgg ttagcgagaa aaaataaagt tatcttttgc taaaaactga aataaaaagg
     1081 ctgagtccgg gtaatatcgg cctcagtctt ttaaattgta gaaaatcatc tgta
//
LOCUS       DANLRK010000280         1130 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.281, whole genome shotgun sequence.
ACCESSION   DANLRK010000280 DANLRK010000000
VERSION     DANLRK010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1130
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.281"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            <1..623
                     /locus_tag="PXA33_002586"
     CDS             <1..623
                     /locus_tag="PXA33_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1595-like element ISMha2 family transposase"
                     /protein_id="HDL5121866.1"
                     /translation="QHKLIELFVAGVTARTAAELVNVNKTTAAYYFHRLRLLIYQHSP
                     HLEMFEGEIEADESYFGGARKGKRGCGAAGKTAVFGLLKRDDKVYTVVVPNTQSATLL
                     PIIREKVKPDSLVYADFYRSYDVLDVSEFNHFRINHSTHFAENHNHINGIENFWSQAK
                     RHLRKFNGIPKAHFELYLKECEWRFNHSDLKSQIFLLKQLVKGGLG"
     gene            complement(822..1061)
                     /locus_tag="PXA33_002587"
                     /pseudo
     CDS             complement(822..1061)
                     /locus_tag="PXA33_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651213.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
BASE COUNT          341 a          226 c          211 g          352 t
ORIGIN      
        1 aacaacataa actcattgaa ctgtttgtag caggtgtcac tgctcgaaca gcggctgagt
       61 tggtgaatgt aaacaaaacc accgctgcct attactttca ccgtttgcga ttgctcatct
      121 atcaacatag ccctcatctg gaaatgtttg agggtgaaat tgaagcggac gaaagttatt
      181 tcggtggtgc tcgcaaaggt aaacgagggt gtggtgccgc aggcaaaact gccgtatttg
      241 ggctgttaaa acgtgatgac aaggtttata ccgttgtcgt accaaatacg caatctgcca
      301 cacttttgcc gattattcgg gagaaagtga agccagacag ccttgtttac gccgattttt
      361 atcgaagcta tgatgtgctt gatgtgagtg aatttaacca ttttcgtatc aatcacagca
      421 cacacttcgc tgaaaatcac aatcacatta acggaattga gaatttttgg agtcaagcaa
      481 aacgtcattt acgcaagttt aatggtattc cgaaagccca ttttgagcta tatttgaaag
      541 aatgcgaatg gcgttttaat cacagtgact taaaatctca aatattcctt ttaaaacaat
      601 tggttaaagg ggggttaggt tagttatcta ggacagcccc aaaaataatg gcataagtga
      661 ctcacttatg ccatttttta tatgctttaa cgtttattaa aaatcttaaa aatattgttg
      721 gcttcctcta aataatgcaa gattttccgc actcatattg ccgttaccaa cacctgctac
      781 gacaatacct tgatagccta catctaacaa ggctttagtg ggccaatttt cacaatctgc
      841 tcacctttga tattcgccaa tgttttcatt tctggcactg cttcaattaa ggtttcgata
      901 ttcaattgcc ctgcttgata agcactacta tttgcattct gctgactacc agcaatcgtt
      961 ccgcctgtcg ctaaaatagt gatattaggt aattctgccg cattagctgt tacaaataag
     1021 ccacctaaca ttgctgctaa tgctaatttt ttgaatttca tataaggctc cttaattaaa
     1081 aaaactaaag acagatgata ccgataaatc aatgtaaatt atttgatctg
//
LOCUS       DANLRK010000281         1103 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.282, whole genome shotgun sequence.
ACCESSION   DANLRK010000281 DANLRK010000000
VERSION     DANLRK010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1103
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.282"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            107..352
                     /locus_tag="PXA33_002588"
     CDS             107..352
                     /locus_tag="PXA33_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121867.1"
                     /translation="MTNDGSIIARGSGFTTYALELENDGLKYLVGFVQPNGNAKEFIE
                     PGATIRRSNDQPSIGSVDCDPRKKIHYKFDVHLMNTL"
     gene            571..>1103
                     /locus_tag="PXA33_002589"
     CDS             571..>1103
                     /locus_tag="PXA33_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4852 domain-containing protein"
                     /protein_id="HDL5121868.1"
                     /translation="MCKKAFPLWGLIFGLLLSTSAFSVSKNFNYQNILMESLHFSENI
                     ELSSLSKDWLLYSDRNTYHKYQNDEFEFQDKIEQAGKKLQKLVDDRKSEEAPIVFTIP
                     VNSEFGNYDFEQGKFEYQPLMNGHMYISSNQYTENLPRKINLYVSNPEFIDGIPMGKD
                     KAKEFLQKRKNSLGNINR"
BASE COUNT          380 a          181 c          213 g          329 t
ORIGIN      
        1 gacttttacc agcacagaag gtaaacctct cacagttaaa cgggtaggaa atgattacga
       61 gtattcgtat gacaaaacta ctttcaaaaa cccgattaaa aaggcagtga ccaacgacgg
      121 ttcgattatc gccagaggct caggttttac tacttatgcc ttagagttgg aaaatgatgg
      181 cttgaaatat ctagtcggat ttgttcaacc aaatggaaat gcaaaagagt ttattgagcc
      241 gggagcaaca attcgccgta gtaatgatca accaagtatt ggttctgtgg attgtgaccc
      301 tcgtaaaaaa atccattata agtttgatgt tcatttgatg aatactttat aatacctttg
      361 tggtgctaga gctagcccaa gttaaaaaag acaaagctcg gcagatgaac aattgtggtg
      421 tcagagctga cccacgttaa aaaacacaat gctcggtagg taaatccctt aggtttaaaa
      481 aatctaaggg atttttgttt aaaagacttg ctctttatta tcaggtggat ctatcataat
      541 ttaaactctc taatgtgtca ggagataaaa atgtgtaaaa aagctttccc attatggggc
      601 ttaatcttcg gattactgct atccacttca gcattctcag tgtctaaaaa cttcaactat
      661 caaaacatat tgatggaatc attacatttt agtgagaata tcgagcttag ttcgttatct
      721 aaagactggt tactttacag cgatagaaat acgtatcata aatatcaaaa tgacgagttt
      781 gagtttcagg ataaaattga gcaagctggt aagaaactac aaaaactggt ggatgacaga
      841 aaatctgaag aagcccctat tgttttcaca attcctgtaa actcagaatt cgggaattat
      901 gattttgaac aaggtaagtt tgagtaccaa cccctgatga atgggcatat gtatatttct
      961 tctaatcaat atactgaaaa tttgccaaga aaaattaatc tgtatgtttc taatcccgag
     1021 tttattgacg gtatacctat gggcaaggat aaagctaaag agtttttaca gaagcgtaaa
     1081 aattctttgg gaaacataaa tcg
//
LOCUS       DANLRK010000282         1100 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.283, whole genome shotgun sequence.
ACCESSION   DANLRK010000282 DANLRK010000000
VERSION     DANLRK010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.283"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(<1..192)
                     /locus_tag="PXA33_002590"
     CDS             complement(<1..192)
                     /locus_tag="PXA33_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121869.1"
                     /translation="MTTFNKILNPMYSTIASYSTQDDGSLNAKYVVGTGDDTDGEVTN
                     FVIITSEYKYIDAQSAKAIT"
     gene            complement(315..>1100)
                     /locus_tag="PXA33_002591"
     CDS             complement(315..>1100)
                     /locus_tag="PXA33_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121870.1"
                     /translation="PMLEEASDNVTDITQPSPWQNAGVTAIHGGSIVTNTVTAQQIAA
                     NTITANEIATGTIAARNMAANSINASHIVGSSITADKLNVNNLAAISANLGKVTAGTI
                     TGTTITGNTISGGSINGTTISGTTISGGTIKGARLEGATGKFTGELEVSQLIGGGVIE
                     QVTGKLKYSHRNRIYEGQEHGENSSSPIYRHEYIYKATVTINAAPTRRYIEIIGSESF
                     ILEANQSITKTIEKSLNRNASGSSLDFIVLAYSISTAKYISIS"
BASE COUNT          285 a          224 c          217 g          374 t
ORIGIN      
        1 agtaatcgcc ttggcggact gtgcatcaat gtatttgtac tcacttgtga tgattacaaa
       61 gtttgttact tctccatcgg tatcatcgcc tgtaccgacc acatatttag cattgagcga
      121 gccgtcatct tgcgttgagt agcttgcgat ggtggaatac attgggttta agattttgtt
      181 aaaagttgtc ataatttttc cttttatcga tagttgaaat aaaaacaatc gcctgaattg
      241 acctgcaagc ggttggtttt ggatatttaa aaaagctgtg gctatccgca ctcagtacta
      301 cattgaggcg ataactaact gatagagata tatttagccg tcgaaatgga ataggctaaa
      361 acaataaaat ctaaagagct accacttgca tttctattta atgatttctc tattgttttt
      421 gtgattgatt gatttgcttc taaaatgaat gactcacttc cgatgatttc aatatacctc
      481 cttgtcggcg ctgcatttat ggtgacagtt gctttataga tatattcgtg tctataaatt
      541 ggagaggagg agttttctcc atgttcttgc ccctcataaa tgcgattacg atgtgaatat
      601 tttaatttgc ctgtaacctg ctctataaca cctcccccta ttaattgaga tacttcaagt
      661 tcgccggtaa atttgccagt agccccctcc aatcttgcac cctttattgt gccgccagag
      721 atagttgtac cgctaattgt tgtaccatta atacttccgc cactaattgt attaccagta
      781 attgtcgtac ctgtaattgt accagcagtt accttaccaa gatttgccga tattgccgct
      841 aaattgttta catttagctt atccgcagtg atcgaactcc ccacaatatg acttgcatta
      901 atcgagtttg ctgccatatt tcgagctgca atcgtgccgg ttgcgatttc gttggcggta
      961 atggtatttg ctgcaatttg ttgagcagta acggtattag taactattga tccaccgtgg
     1021 atagcggtta caccggcatt ttgccaaggg ctaggctgag tgatatctgt aacattatca
     1081 ctagcctcct caagcattgg
//
LOCUS       DANLRK010000283         1075 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.284, whole genome shotgun sequence.
ACCESSION   DANLRK010000283 DANLRK010000000
VERSION     DANLRK010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.284"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(319..1062)
                     /locus_tag="PXA33_002592"
     CDS             complement(319..1062)
                     /locus_tag="PXA33_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121871.1"
                     /translation="MLNKHINKIINSSFSLAFFTLTSFATAYCYGWGQAIFHGYPWWH
                     VEIGNASMARSLAYVFGTSVILFLCYALGYSLLNKVFQLHYFRNLGWLRVIVLVSVFT
                     VPVILTFYLFTGIVPIYISFIYIFVSGVSVFFFRKRWNNTKFDLDFRKMLLGGSFALF
                     NIFIFLYFSLLSLHIGYLRAGLRTTYDYIEIENQKYYILSTNSDNGYIMGTKIRGNQS
                     FLFFNRDTHQYYHVHIEQCTDSRLCKVIV"
BASE COUNT          380 a          197 c          180 g          318 t
ORIGIN      
        1 tttaatcgag aattatgttc ttttttattt tgttattctc tttaggtata aaaaaacccg
       61 gtaaataccg ggcttttatt tcaattggtt atccaacttg cgagagcaat taccgcaata
      121 aaaataacta accaaataag ttgtaatttt acgcttttgc ggttgatctc ctggttgtgt
      181 aatcggcgct gttctaactt ttgccgatat atcgctaaat cggcttcttt aaacgagaag
      241 ccacaatgta tgcaatgttc taaactgtcg ctaatacgct ttcgacactc cggacaacgg
      301 tgtaatgcca taattacctc aaactattac tttgcataat cggctatccg tacattgttc
      361 aatatgaaca tggtagtatt gatgagtatc acgattgaaa aacaagaaag attgattacc
      421 tcttattttt gttcccataa tgtagccgtt atcactgttg gtggataata tgtagtattt
      481 ttgattctct atttcgatgt aatcataggt agttcttagt ccggcacgca aataaccaat
      541 atgcaatgag agcaaactga aatagagaaa aataaagatg ttaaacaggg caaaacttcc
      601 tcctaatagc atctttctaa agtccagatc aaacttggta ttattccatc tcttgcggaa
      661 gaagaatacg ctcacaccac ttacaaaaat atagataaaa gagatataaa tgggcactat
      721 cccggtaaat aagtaaaaag taagaataac aggcactgta aacacagaaa ccaacacaat
      781 cacccttaac caacctagat ttcggaaata atgcagctga aaaactttat ttagtaaact
      841 ataacctagt gcataacaca agaataagat gacggaagta ccgaatacat aagccaaact
      901 tcttgccata ctggcattgc cgatttcaac atgccaccaa gggtagccat gaaatatggc
      961 ttgtccccaa ccgtaacagt aagcggtagc aaaactagtt aatgtaaaaa atgcaagaga
     1021 aaatgaagaa ttgataattt tattaatgtg tttatttagc ataacaaaga atgtc
//
LOCUS       DANLRK010000284         1047 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.285, whole genome shotgun sequence.
ACCESSION   DANLRK010000284 DANLRK010000000
VERSION     DANLRK010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1047
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.285"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            <1..>1047
                     /locus_tag="PXA33_002593"
     CDS             <1..>1047
                     /locus_tag="PXA33_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121872.1"
                     /translation="SLADRDEIWTAFIALESDQCPAIERLLTWCQKSSGQENIEPEAL
                     LLGAIAVSWLFTSTDKRSRNGYINALAHLLWNRLEIALALFKEFIEVNDPYVMEGILS
                     AIHGAVAYSENSSGLKALALEIDNQIFNQPETEEIYPNVLVRDYARHIIEYSLQKDEY
                     SSDDIEIIRERITPPYRSSFPQNLPTNEEIDKKYHSDGQRIIIRSMTTEYGRGMCGYG
                     DFGRYTFEAKFRNWESYKFGNLINVDLLSNYACQMIFEQFGYDDKKHLEFDKETRSRY
                     WRRDDTNKIERIGKKYQWLALYEILARVMDNFPTFSNRYADEIGLEYITNLDNFYLPK
                     TQIKIVPPTKTDVF"
BASE COUNT          350 a          155 c          201 g          341 t
ORIGIN      
        1 tttctttggc agatagagat gagatttgga cagcttttat tgctttggag tctgatcaat
       61 gcccagcaat tgaacgatta ctaacttggt gtcagaaatc aagtggtcaa gaaaatattg
      121 aacccgaagc actattattg ggtgcaattg ctgttagttg gctatttact tctaccgata
      181 aacgaagtag aaatggctat attaatgcac tggctcattt attatggaat agattggaaa
      241 ttgcattggc gttattcaag gaatttatag aggttaatga cccttatgtt atggaaggta
      301 ttttatctgc cattcacgga gctgttgctt attctgaaaa ttcatctggc ttaaaagcac
      361 ttgcattaga gatagataat caaatattta atcaacctga aacagaagaa atttatccca
      421 atgttttggt tcgtgattat gctcgccata ttattgaata ctctttgcaa aaagatgaat
      481 attcatcaga tgatattgag ataatcagag aaagaattac gccaccttat agaagttcat
      541 ttccccaaaa cttaccaacc aatgaagaaa ttgataaaaa atatcattct gatggacaaa
      601 gaatcattat ccgttcaatg acaactgaat atggtcgtgg tatgtgtgga tatggtgatt
      661 ttggcagata tacttttgaa gccaaattcc gaaattggga gagttataaa ttcggcaatc
      721 tcatcaatgt tgatttatta agtaattatg cttgccaaat gatttttgag caatttggct
      781 atgatgacaa gaagcacctt gagtttgata aagaaactcg ttcaagatat tggcgaagag
      841 atgatactaa taaaattgag cgaattggca agaaatatca atggttggct ttgtatgaaa
      901 tcttggcaag ggtgatggat aattttccta ctttttctaa tcgctatgcc gatgaaatag
      961 ggcttgaata tattactaat ttggacaact tttatctacc caaaacccaa attaaaattg
     1021 taccgccaac taaaacagat gtatttt
//
LOCUS       DANLRK010000285          983 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.286, whole genome shotgun sequence.
ACCESSION   DANLRK010000285 DANLRK010000000
VERSION     DANLRK010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..983
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.286"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            136..>983
                     /gene="msrA"
                     /locus_tag="PXA33_002594"
     CDS             136..>983
                     /gene="msrA"
                     /locus_tag="PXA33_002594"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005546455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDL5121873.1"
                     /translation="MKYNQLLLCLVSAIFTTSSIANITTPNEMRSENLQDIYLAGGCF
                     WGIEAYMEKIYGVKEATSGYANGKTEQTAYRIIGQTDHAETVHVTYDANKISLEKLLQ
                     YYFKVIDPTSKNKQGNDIGRQYRTGIYYQNEQDKQIILKEIQAQQAKYKQKIQVQVEP
                     LKHYILAEEYHQDYLKKNPNGYCHIDLNQANSIIIDPKDYPKPSDATLKMKLTPLQYS
                     VTQRKNTERSFSNEYWDNTAPGIYVDITTGEPLFSSLDKYDSGCGWPSFTKPIINEVV
                     NYTEDN"
BASE COUNT          380 a          176 c          164 g          263 t
ORIGIN      
        1 taaacaatat tcgactaatc ctatagagag taaaaccaat tttgatctta gcaaaaaagt
       61 aagaaattat gaatgtttaa ggttaaaccc tcatccttaa aaaataaaat tacataagtt
      121 ataaaggaga actggatgaa atacaatcaa ctgttactat gtttggtgtc tgctatcttt
      181 accacctcct caatagcaaa catcacaacc cctaacgaga tgcgatctga aaacttacag
      241 gatatttact tggcaggagg atgtttctgg ggcattgaag cctatatgga aaaaatatat
      301 ggcgtaaaag aggctacatc gggctatgcc aatggcaaaa ctgagcaaac tgcttaccga
      361 attatcgggc aaaccgatca tgcagaaaca gttcacgtga cctatgatgc taataaaatt
      421 tctttagaaa agctgttgca atattacttt aaagtaattg atccaaccag taaaaataaa
      481 caaggtaatg atattggccg ccaatatcgg acaggtattt attatcagaa tgagcaagat
      541 aaacaaatta ttttaaaaga aatacaagct caacaagcaa aatacaaaca aaaaatacaa
      601 gtgcaagtag aaccattaaa acattatatt ttggcagaag agtatcatca ggattaccta
      661 aagaaaaatc caaatggcta ctgccatatt gatttaaacc aagccaacag cattattatc
      721 gatccgaaag attatcctaa acctagtgat gcaacattaa aaatgaaatt aacgcctttg
      781 caatatagcg ttacccaacg taaaaatact gagcgttcat tcagcaatga atattgggat
      841 aataccgcac cgggcattta tgttgatata actacaggag agcctctttt ttcatctctc
      901 gataaatatg actccggctg tggctggcca agttttacca aaccaattat caatgaggtt
      961 gttaactata ccgaagataa tag
//
LOCUS       DANLRK010000286          970 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.287, whole genome shotgun sequence.
ACCESSION   DANLRK010000286 DANLRK010000000
VERSION     DANLRK010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..970
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.287"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            128..376
                     /locus_tag="PXA33_002595"
     CDS             128..376
                     /locus_tag="PXA33_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121874.1"
                     /translation="MISGEKMTWESLGASAIGGAIGGGIGATMKAGAVTASTLSALGG
                     GMGEGLYKSTSQGIKNSIGIKASCNQSGSDYGDGCDYQ"
     gene            388..591
                     /locus_tag="PXA33_002596"
     CDS             388..591
                     /locus_tag="PXA33_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121875.1"
                     /translation="MKIQFPISYQEFRENYFEKKPLLMKGAISQKDLLSWKSINEILP
                     RCDLISEDAIKVMHKGKRAHKKD"
     gene            630..>970
                     /locus_tag="PXA33_002597"
     CDS             630..>970
                     /locus_tag="PXA33_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_046701250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL5121876.1"
                     /translation="MKITHCKLKKSLQKKLLEFFVAEVTARTAADLLGIQPNTAALFY
                     HKIRLVIEHHLALEAHEIFEGKIEVDESYFGGHRKGKRGRGAAGKVAVFGLLKRQGKV
                     FTKYQIRNANAG"
BASE COUNT          313 a          134 c          239 g          284 t
ORIGIN      
        1 ataaacatca tagaggaata tctcttatga aagagttaaa aattgaggat tgccaatttg
       61 tttcggcagg cttgcacttg ttattgtagg agctgttgct ggagctgcat attatgttgg
      121 gaatggtatg atttctgggg aaaaaatgac ttgggagagt ttgggagcat cagctatagg
      181 tggggctatt ggtggcggta ttggagcaac aatgaaagct ggtgcggtga cagcgtcaac
      241 cttatctgct ttaggtggtg gtatggggga gggcttgtat aaaagtactt ctcaaggtat
      301 caaaaatagt attggtataa aggcgagttg caatcaatca ggttcggatt atggtgatgg
      361 atgtgattat caataagaga gtatattatg aaaattcaat ttccaatttc ttatcaagaa
      421 tttcgtgaaa actattttga gaaaaaacca cttttaatga aaggagcaat atctcagaaa
      481 gatttacttt cttggaagtc aataaatgaa atattacctc gctgtgattt aatttctgag
      541 gatgcaatta aggtaatgca taaagggaaa agggctcata aaaaagatta ggggctgtag
      601 tagattagca tcactgctat actacaaaaa tgaagataac ccattgtaaa ttaaagaaat
      661 ctttgcagaa aaagcttctt gaattttttg ttgctgaagt gaccgctaga accgctgctg
      721 atttattagg tattcaaccc aatacggcag ccctgtttta ccacaaaatt aggctggtca
      781 ttgaacacca tttagccctt gaagctcatg aaatttttga gggaaaaatt gaggtagatg
      841 aaagctattt tggtggtcat cgcaaaggaa aacgagggcg aggtgcggct gggaaagtcg
      901 ctgtattcgg tttgttgaag cgtcaaggaa aggtgtttac aaaataccaa atcagaaacg
      961 ctaatgccgg
//
LOCUS       DANLRK010000287          966 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.288, whole genome shotgun sequence.
ACCESSION   DANLRK010000287 DANLRK010000000
VERSION     DANLRK010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..966
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.288"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            <1..296
                     /locus_tag="PXA33_002598"
     CDS             <1..296
                     /locus_tag="PXA33_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121877.1"
                     /translation="FALPVQADSLELARNKVKQIFISDEEPKVKDATWTAPFIFKVGV
                     FDDGTKRDGYAEYVCQVLYDHGFKGKGVLVSVIDIKKLVQTGDWVDLGKAKCQ"
     gene            396..656
                     /locus_tag="PXA33_002599"
     CDS             396..656
                     /locus_tag="PXA33_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excalibur calcium-binding domain-containing
                     protein"
                     /protein_id="HDL5121878.1"
                     /translation="MKKLVLSILFVISPMALAYSCNDPIPYCKEMQSCEQAKFYLNQC
                     NAGRLDRDNDGIPCENVCGKKGKSEKKDDKKSDKSKSKKTSN"
     gene            688..>966
                     /locus_tag="PXA33_002600"
     CDS             688..>966
                     /locus_tag="PXA33_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HDL5121879.1"
                     /translation="MMNNQSVWHLFCDESGISGKPFYAFGALWIREDKISLFEKEIQK
                     LREKHYCTDEIKWQGTNSKRFSEFYRELVSFFFQADYLFFNCIVVQQAI"
BASE COUNT          329 a          139 c          195 g          303 t
ORIGIN      
        1 tatttgcatt gcctgtacaa gccgatagcc tcgaacttgc tcgtaataaa gtaaagcaaa
       61 tttttatcag tgatgaagag ccaaaggtaa aagatgcgac ttggactgca ccatttatct
      121 ttaaggttgg agtttttgat gacggcacga aaagagatgg ctacgccgaa tatgtttgcc
      181 aagttttata cgaccacgga tttaaaggta aaggtgtgtt ggttagcgta atcgatataa
      241 aaaaactagt ccaaacaggt gattgggttg atcttggcaa agctaaatgt cagtaataca
      301 taagggacag attattctgt ctctttgtat ttaatcatta atgaagaaac ctttttacta
      361 cacagttaat actatatttt ataatggggt tcataatgaa aaaattagtc ctctcgattt
      421 tatttgttat ttcgcctatg gctttggctt atagctgcaa tgaccctatc ccttactgca
      481 aagaaatgca atcttgcgag caagcaaagt tttatttaaa tcaatgcaat gccggtcgat
      541 tagatagaga taacgatggc ataccttgtg aaaatgtttg tggcaagaaa ggaaagtccg
      601 agaaaaaaga cgataagaaa tcagataaat caaaatctaa aaaaacttca aattagagac
      661 aggcgttgtg cctgattaaa aggggtaatg atgaataacc agtcagtttg gcacttattt
      721 tgcgatgaat ccggtataag cggtaagcct ttttatgctt tcggtgcatt atggataaga
      781 gaagataaaa ttagcttatt tgaaaaagaa atacagaaat tacgagaaaa gcactattgc
      841 actgatgaaa taaaatggca aggtacaaat tctaaacgct ttagtgaatt ttatcgtgaa
      901 ttggttagtt ttttctttca ggctgattat ttatttttta actgtattgt agttcaacaa
      961 gctatt
//
LOCUS       DANLRK010000288          964 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.289, whole genome shotgun sequence.
ACCESSION   DANLRK010000288 DANLRK010000000
VERSION     DANLRK010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..964
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.289"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            223..964
                     /locus_tag="PXA33_002601"
                     /pseudo
     CDS             223..964
                     /locus_tag="PXA33_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007524908.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein"
BASE COUNT          343 a          167 c          151 g          303 t
ORIGIN      
        1 gactcctcta atagtccact cttgttccat tttggaacat ttttgtatat tatttcgcct
       61 tttctttctc attgtgttct atgtaggcaa cagcattttt tgcaaagtga tcgacgagaa
      121 cattcattac ttcagtccat tttacaggtc ttcctatctt cattccaact tccatcgcta
      181 atctttctat cttgagtttt ttctctaatt taatattgaa cagagcttta tgaaatggtt
      241 gatatagaac aaacgtcagt tgattggatt gatgtaactt attcagctca tattgattcg
      301 gaatctatcc aaaaacaact tatctctttt ttccaaaaca tccatttagg acaaacacgt
      361 cgtacaagac ataacaaaga atatgacaca acggcagaat ggaattcagg ttcagaacac
      421 cgtattttaa aagtttatct aaaaggtaat gaagttcaaa aaagactaca agaagcccag
      481 cacgacttca aaaaatcacc aagtaaaaag tatctgcaaa actcaattca agtattaagt
      541 aatccaaatc tcattgaatt ttcaaaaaaa tgtgttcgtt ttgaagcaag attaaaacaa
      601 cgttatttag ataaacatca aatcccacgc aatttatttg acttaattaa atatcagaga
      661 gattacgaag agcagggtag aagcctgatt aaagacctat ggcaagacgc attcaaagac
      721 attattaagg ctgtcgggga aagcaatatg aatatttaca acagagatca aattcagcgt
      781 ttattacgga aaaattattt ttcaatcact ccaaaaggaa atatgagcta tgcaaaggct
      841 gatcgtttat tcagctttta taagaattta ctaagtgatg gctatagaga aacgctagat
      901 tctatgccaa gaaacacatt ttggagatat gaaaaggatc ttatggccgt tggtttaact
      961 aaaa
//
LOCUS       DANLRK010000289          932 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.290, whole genome shotgun sequence.
ACCESSION   DANLRK010000289 DANLRK010000000
VERSION     DANLRK010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..932
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.290"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            68..463
                     /gene="mscL"
                     /locus_tag="PXA33_002602"
     CDS             68..463
                     /gene="mscL"
                     /locus_tag="PXA33_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HDL5121880.1"
                     /translation="MSILKEFREFAVKGNVVDMAVGVIIGGAFGKIVSSLVSDVVMPP
                     IGWLIGGVDFKDLAIEIAPAKEGAEAVMLKYGAFIQNVFDFLIIALAVFGMVKVINSL
                     KKAPVVEEAPAEPTAEEKLLTEIRDLLKK"
     gene            complement(576..839)
                     /gene="rpsT"
                     /locus_tag="PXA33_002603"
     CDS             complement(576..839)
                     /gene="rpsT"
                     /locus_tag="PXA33_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HDL5121881.1"
                     /translation="MANIKSAKKRAVQSEKRRQHNASQRSMMRTFIKKVYAAVAAGDK
                     AAAQTAFVEMQKVVDRMASKNLIHANKAANHKSKLAAQIKKLA"
BASE COUNT          263 a          151 c          200 g          318 t
ORIGIN      
        1 gattataatc gccagcccac gttcttgcga agaccgtgtt taatcattga atgaaaggaa
       61 agcccccttg agtattttaa aagagttccg tgaatttgcg gtaaaaggta atgttgttga
      121 tatggctgtc ggtgtgatta tcggcggtgc attcggtaaa attgtttctt cattagttag
      181 cgatgttgta atgccaccta tcggttggtt aatcggtggc gtggatttca aagatttagc
      241 cattgaaatc gccccggcaa aagaaggtgc ggaagcagtg atgttaaaat acggtgcatt
      301 tatccaaaac gtatttgatt tcttaattat cgcacttgcg gtattcggta tggtgaaagt
      361 gattaactca ctgaaaaaag ctccagttgt agaagaagct cctgcagagc caacagcgga
      421 agagaaatta ttaactgaaa tccgtgattt attgaaaaaa taattaactg attttgaaac
      481 aagggctatt ttagaatatc tagaatagcc ctttgtgtta tggttaaaaa gaaaaaaacc
      541 aacataaagt cggttttttt catatctgtt cgatattacg ctaatttttt gatttgcgct
      601 gctaatttag atttgtggtt agccgctttg ttagcgtgga ttaagttttt agatgccata
      661 cggtctacaa ctttttgcat ttcaacgaaa gccgtttgag ctgctgcttt atcgcctgct
      721 gctactgctg catatacttt tttgatgaat gtacgcatca ttgagcgttg gcttgcgttg
      781 tgttggcggc gtttttcaga ttgaaccgca cgtttttttg ctgacttgat attagccaag
      841 gtcaaactcc taaaatatct gtgtaaaaat tagatataaa caaaatcgtt gtttatattg
      901 cctatcgata gtttttaatt cattttcaac ga
//
LOCUS       DANLRK010000290          920 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.291, whole genome shotgun sequence.
ACCESSION   DANLRK010000290 DANLRK010000000
VERSION     DANLRK010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..920
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.291"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            <1..862
                     /locus_tag="PXA33_002604"
     CDS             <1..862
                     /locus_tag="PXA33_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HDL5121882.1"
                     /translation="TYMNDNVIPFMEKVEEFTETLKTLSRDDLSAEERTTKTIDSVKL
                     RIYLNEQARNRKNYINGFVPTDWIPEITDQMTKSKNLWLKYEEEAKTTLKPYQEETIS
                     RVIQDEINKKMAEIGTTTLEIGGKEHELEDLENQINALALTDEEKLAEDVENDLISKL
                     DEAKAALAKNQQTLKEKRDELLGLNNKLDSSPLVKKGKNALAEGTNAFASGENAIAFG
                     TDSQATGNNAIALGANSKANAESAIAIGKGAQALKEKALALGENAIANRASAIAIGDN
                     HSSKIVEDQT"
BASE COUNT          365 a          156 c          171 g          228 t
ORIGIN      
        1 cacttatatg aatgataatg taattccttt tatggagaag gtagaggaat ttactgaaac
       61 attaaaaact ttatctagag atgacttaag tgcagaggaa cgtacaacca aaacaattga
      121 ctccgttaaa ttaagaatat atctcaatga acaagcacga aatcgtaaaa attatattaa
      181 tggatttgta cctaccgatt ggattcctga aattacagat caaatgacaa aatccaaaaa
      241 tctatggtta aaatatgaag aggaagccaa aacaacatta aaaccatacc aagaagaaac
      301 aatatctagg gttatacaag atgaaattaa taaaaaaatg gctgaaatag gtactacaac
      361 actagagatt ggtggtaaag aacacgagct cgaggatctt gaaaaccaaa ttaatgcttt
      421 agctttaact gatgaagaaa aacttgcgga agatgtggaa aatgatttaa tctcaaagct
      481 tgatgaggct aaagcagcct tagcaaaaaa ccagcaaacg ctcaaagaaa agcgtgatga
      541 attactaggg ctaaataaca aattagatag ctcaccactt gttaaaaaag gtaaaaatgc
      601 acttgcagaa ggcacgaatg cctttgcttc tggtgaaaat gccatcgcat tcggcacgga
      661 ttcacaagct acaggtaata acgctatcgc actaggtgca aattcaaaag caaacgcaga
      721 aagtgcgatt gcaattggta aaggtgcaca agcattaaaa gaaaaagccc ttgctttagg
      781 cgaaaatgcc attgccaaca gagcatctgc tattgcgatt ggtgataacc attcatctaa
      841 aattgtagaa gatcaaacct aatctgacag tcccccgttt taaattaccg tgtctgtcag
      901 attaatttga gcttaaattc
//
LOCUS       DANLRK010000291          884 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.292, whole genome shotgun sequence.
ACCESSION   DANLRK010000291 DANLRK010000000
VERSION     DANLRK010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..884
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.292"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            19..198
                     /locus_tag="PXA33_002605"
     CDS             19..198
                     /locus_tag="PXA33_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module killer protein"
                     /protein_id="HDL5121883.1"
                     /translation="MRIATGAGYRIYYAQQGNVIYILLHGGDKSTQQADIEKAKALWA
                     ELKTTGESNGNNTNQ"
     gene            176..499
                     /locus_tag="PXA33_002606"
     CDS             176..499
                     /locus_tag="PXA33_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative addiction module antidote protein"
                     /protein_id="HDL5121884.1"
                     /translation="METIQINEQAAEKHGLRPFDVAEFLTDEATIQAYLAEVLKEGDQ
                     DEFLEALNNVARARGMAELAKQTGLGRESLYKTLAKGSKPRFDTIQKILGAFNLVLIP
                     QVKNG"
BASE COUNT          311 a          172 c          182 g          219 t
ORIGIN      
        1 acaggcggac tttatgaaat gcggatagca acaggagccg gatatagaat ttattacgca
       61 caacaaggaa atgtaattta tatcttgtta cacggcggcg ataaatcaac acagcaagcc
      121 gatatagaga aagcgaaagc attatgggct gaattaaaaa ctacagggga aagcaatgga
      181 aacaatacaa atcaatgaac aggcagcaga aaagcacggt ttacgccctt ttgacgtggc
      241 ggagttttta accgatgagg caactattca agcctattta gcggaagtgt taaaagaggg
      301 cgaccaagac gaatttttag aagccttgaa caatgtggca agggcaaggg gaatggcgga
      361 gcttgccaag caaacagggc tagggagaga gagcctatat aaaaccctag ccaaaggcag
      421 caaaccacgc tttgacacta ttcaaaaaat tctaggggca ttcaatttag tgcttattcc
      481 acaagtcaaa aatggctaaa acttatcact tgctacctgc caaaacctca cattcaaata
      541 gccgttgatt gatattaacg gcttttttat tgcttaattc tgtaccaatt acgcaccgat
      601 tgcggatcaa tacggggcaa gttttcgcaa aaccttaatg aaatatgacc gcttgtgatt
      661 ttgtacaaaa cgacacaaac caatgtataa aatcaaaact tgccacaaat ggcggaatga
      721 tattaaatgc cccacaaact agcagtattt cttaatgact tttcatttta agggtacatt
      781 taagggtatg tttaacaagt cttaaatgca cttctcaata ataacaagcc tcatagcata
      841 caattcaaac tcggctgggg accatttaag cgttgcatat tgtc
//
LOCUS       DANLRK010000292          863 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.293, whole genome shotgun sequence.
ACCESSION   DANLRK010000292 DANLRK010000000
VERSION     DANLRK010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.293"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            141..>863
                     /locus_tag="PXA33_002607"
     CDS             141..>863
                     /locus_tag="PXA33_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HDL5121885.1"
                     /translation="MLNWAGKSTAYQTLQTPTSKTLTPCEEESVDFENTQNIFIEGEN
                     LDVLKALQKSYFNSVKMIYIDPPYNTGNDFIYNDSFADTKADYAEKVGDVDEHGKLKR
                     AFVRNSKENGHYHSNWLNMMLPRLHLAKNLLRDDGVIFISIDDNEQAQLKLLCDEVFG
                     EENFVAILSVENNPKGRKNSKFISVSNDFCIIYAKNKEMGKFVENIPKNAKDMCQDEN
                     GHYVHASGKRVLVGENKFNQPVT"
BASE COUNT          299 a          147 c          166 g          251 t
ORIGIN      
        1 ttgccaactt ttcgcttaaa gatcaacaac tttctcaatt aaaatccctt ttacctgaaa
       61 ttttcaccga aggtaaaatc gacttcgaca aattccaaca acttttttcg gataattttt
      121 ccaccgaacc tgaccgctat atgctcaact gggcaggaaa atcaacagcc tatcaaacct
      181 tacaaacccc aaccagcaaa acgctcactc cttgcgagga agagagtgtg gattttgaaa
      241 atacgcagaa tatctttatt gagggtgaaa atttagatgt attaaaagcc ctgcaaaaat
      301 cctacttcaa ttcggtcaaa atgatttata tcgatccgcc atacaataca ggcaacgatt
      361 ttatttataa cgacagtttt gccgatacca aagcagatta tgcggagaaa gtgggcgacg
      421 ttgatgaaca cggcaaattg aaacgagcct ttgttcgtaa cagcaaagaa aatgggcatt
      481 atcacagtaa ctggctgaat atgatgttgc ctcgtttgca tctcgcaaaa aatttactgc
      541 gtgatgacgg ggtgattttt atttcaattg atgataacga acaggcacag ctgaaattgc
      601 tttgtgatga agtgtttggg gaggagaatt ttgttgccat attatcagtt gaaaataatc
      661 cgaaaggtag aaagaattct aaatttattt ctgtaagtaa tgatttttgt attatttatg
      721 ctaaaaataa agaaatgggg aaatttgttg aaaatattcc taaaaatgct aaggatatgt
      781 gtcaagatga aaatggacat tatgttcacg caagtggaaa acgagtatta gttggggaaa
      841 acaaatttaa tcagcctgta aca
//
LOCUS       DANLRK010000293          853 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.294, whole genome shotgun sequence.
ACCESSION   DANLRK010000293 DANLRK010000000
VERSION     DANLRK010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..853
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.294"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            68..586
                     /locus_tag="PXA33_002608"
     CDS             68..586
                     /locus_tag="PXA33_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein"
                     /protein_id="HDL5121886.1"
                     /translation="MQKKLFIAQIKQNLSELNVFSTDNIFLNSPYFSQQTGLVSVFIA
                     EIEKTVELLLNQTEVLYSEFYAEKLVKQVDALKNAVEKIQSKPESAQFHSSYQFSPNI
                     HRLAPNKRLQEYRKALRALNEKISWLVEQNLNTQNEATKQTLQNQITETEYRKMKCLK
                     AIEDLEQELLFK"
     gene            complement(590..663)
                     /locus_tag="PXA33_002609"
     tRNA            complement(590..663)
                     /locus_tag="PXA33_002609"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(629..631),aa:Cys,seq:gca)
     gene            complement(679..754)
                     /locus_tag="PXA33_002610"
     tRNA            complement(679..754)
                     /locus_tag="PXA33_002610"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(719..721),aa:Gly,seq:gcc)
BASE COUNT          301 a          160 c          150 g          242 t
ORIGIN      
        1 tttgctttaa aatagaagtt atatgaaata aaattcactt aaattttctt tattgataac
       61 atcaaccatg caaaaaaaac tttttatcgc ccaaataaaa caaaacctct cggaattaaa
      121 tgtattttca acggataata tttttctgaa ttcaccctat ttctcacagc aaaccggttt
      181 ggtgagtgtg ttcattgctg aaattgaaaa aacagttgag ctattattga accaaactga
      241 ggtgttatat agtgaatttt acgctgaaaa attggttaaa caagttgatg cattaaagaa
      301 tgccgtagaa aaaatccaaa gtaagccaga atcagctcaa ttccattctt cctatcaatt
      361 ttctccaaat attcatcgtt tagcgcccaa caaacgctta caagaatacc gtaaagcgtt
      421 acgtgcattg aacgagaaaa ttagctggtt agttgagcaa aatttaaata cgcaaaatga
      481 agcaactaaa caaaccctgc aaaatcaaat tacagagaca gaatatcgca aaatgaaatg
      541 tttaaaagca attgaagatt tagaacaaga gttgctattt aaatagtgat ggaggcgtgt
      601 cccggagtcg aaccgagcta catggatttg caatccagtg cataaccgct ttgctaacac
      661 gccataagat ttggaaattg gagcgggaaa cgaggctcga actcgcgacc ccgaccttgg
      721 caaggtcgtg ctctaccaac tgagctattc ccgcatctga attcccacaa cattgcgctg
      781 tggaacgagg tgcattttac ggatttatca ttttatgtca atgctaaatt taacattcta
      841 gattcaaatg ctg
//
LOCUS       DANLRK010000294          852 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.295, whole genome shotgun sequence.
ACCESSION   DANLRK010000294 DANLRK010000000
VERSION     DANLRK010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..852
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.295"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            <1..>852
                     /locus_tag="PXA33_002611"
     CDS             <1..>852
                     /locus_tag="PXA33_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121887.1"
                     /translation="AIAIQNSVNYLYEIAERQPPCNFELCWKNCAAHYLAIKSAESNN
                     ERDLNLFLDYINEPKDSTFTVAPLYKDNTLETRMILEIIKDDNQIGFGFCEASDLSLI
                     EDETKKIKAAMSLIDDLDPDTSNEIKCFIDTIYLTKEGLNGERFMRSGTNFYMWGMMF
                     LYINKEHSILYYADHIIHECAHTALNIINATDELVTNSADERFKAPLRKDTRPMIGLF
                     HALFVLHRICSSFQKMKEKIDDVYYDEFNHRLNDNLLKLHETYQTVRENAKTTEIGEK
                     ILNEIAKT"
BASE COUNT          315 a          113 c          148 g          276 t
ORIGIN      
        1 gctatagcaa tccaaaattc tgttaattat ttatatgaaa ttgcagaacg gcagcctcct
       61 tgtaattttg aactatgctg gaagaattgt gcagcacatt atttagcaat aaaatctgca
      121 gaaagtaata atgaaaggga tttaaattta ttccttgatt atattaatga gccgaaagat
      181 tctacattta cagtagctcc tttatacaag gataatacac tagaaacaag aatgatctta
      241 gaaattatta aagatgataa ccaaattggg tttggatttt gtgaggctag tgatttatct
      301 ttaattgagg atgaaactaa aaaaatcaag gcagcaatga gcttaattga tgatttagac
      361 cctgatactt ctaacgagat aaaatgtttc attgatacaa tttatttaac aaaagaaggc
      421 cttaatggcg agcgatttat gcgttctggc acaaattttt atatgtgggg aatgatgttt
      481 ttgtatataa ataaggaaca ctctattctt tattatgcag atcatataat tcatgaatgt
      541 gctcatacag ctcttaacat tataaatgca actgatgaat tagtaacaaa ttcagctgat
      601 gaacgtttta aagcaccttt aagaaaggat actcgtccta tgattgggct atttcatgcg
      661 ttgtttgtac tccatagaat atgttcctcc tttcaaaaaa tgaaagaaaa gatagatgat
      721 gtttattatg atgaatttaa ccatagatta aatgataacc tgttgaagtt acatgaaaca
      781 taccagactg ttagagaaaa tgcaaaaacg acagagattg gtgagaaaat tttaaatgaa
      841 attgccaaaa ct
//
LOCUS       DANLRK010000295          809 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.296, whole genome shotgun sequence.
ACCESSION   DANLRK010000295 DANLRK010000000
VERSION     DANLRK010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..809
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.296"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            <1..124
                     /locus_tag="PXA33_002612"
     CDS             <1..124
                     /locus_tag="PXA33_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CMP-N-acetylneuraminate-beta-galactosamide-
                     alpha-2, 3-sialyltransferase"
                     /protein_id="HDL5121888.1"
                     /translation="LNKSNHIEVVALRVNVDNPAYIESYELLEKLGVQIVDIRE"
     gene            126..>809
                     /locus_tag="PXA33_002613"
     CDS             126..>809
                     /locus_tag="PXA33_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HDL5121889.1"
                     /translation="MKKYLISLEKDVKRRELFFSQPNTEDFEVFSAINTMSSDWEALN
                     NHFDLALFEQRYGRKTTKGEVGCTLSHLAVYQKIAEDSTIAEDDYCLVCEDDALFADD
                     FQKHLDALLAQNLKVDIILVGQSKIPTFNDSELELNYPSTFGFLQKNIPNSQYKYAYS
                     YLNYFAGTVAYLIKKSTACQFLAQCAGKNPYWLADDFILFEKNFKFDVLIVRPLMAIE
                     NPVLISNLED"
BASE COUNT          271 a          114 c          156 g          268 t
ORIGIN      
        1 tttaaataaa agtaaccata ttgaagtggt agcattaaga gtgaatgtag ataatcctgc
       61 ttatattgag agctatgaat tgcttgaaaa attaggcgtt cagattgttg atattagaga
      121 ataaaatgaa gaaatattta atttcattag aaaaagatgt aaaaagacga gagctatttt
      181 ttagccagcc taatacggaa gattttgagg tgttcagtgc cattaataca atgagtagtg
      241 attgggaggc attaaataac cattttgatt tggctctgtt tgaacagcga tatgggcgta
      301 aaaccactaa gggcgaagta ggttgtactt taagccattt agccgtttac caaaaaattg
      361 ccgaagatag cactattgcg gaagatgatt actgcctagt ctgtgaagat gatgccttat
      421 ttgccgatga tttccaaaaa catcttgatg ctttattagc acaaaattta aaagtcgata
      481 ttattttagt tgggcagtcg aaaattccaa cttttaacga tagcgaatta gagcttaatt
      541 acccatcaac ctttggcttt ttgcaaaaaa atattccgaa tagccaatat aaatatgctt
      601 attcttacct gaattatttt gctggtacgg ttgcatattt aattaaaaaa tcgaccgctt
      661 gtcagttttt agcacaatgt gccggaaaaa atccatactg gcttgccgat gattttattt
      721 tatttgaaaa gaatttcaaa tttgatgtgt taattgtccg cccgttaatg gcgatagaaa
      781 atcctgttct aattagtaat ttggaagat
//
LOCUS       DANLRK010000296          794 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.297, whole genome shotgun sequence.
ACCESSION   DANLRK010000296 DANLRK010000000
VERSION     DANLRK010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..794
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.297"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            <1..>794
                     /locus_tag="PXA33_002614"
     CDS             <1..>794
                     /locus_tag="PXA33_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenine methyltransferase"
                     /protein_id="HDL5121890.1"
                     /translation="DKEIYSSSSYNESQLRTDFLDPFFGLLGWDITNSKNKLTSEREV
                     IVEEPLKEKVSENVKKPDYTFRFNSQRKFFVEAKKPSVKIKTDPLPAHQVRRYGFTAK
                     LSISVLSNFEYLAIYDCSEIVDVQQDALYARVRLYHYTEYVKFFDEIMNLLGRESVYS
                     GHFDNEWREISQKIENSKQTVDELFFEQINKWRHVLGSELLKINPCISNNELNDAVQD
                     YINSIIFLRVCEDRSIECYESLLDVTKLEKHKKFKTNLEKADKKYN"
BASE COUNT          306 a          105 c          131 g          252 t
ORIGIN      
        1 gtgataaaga aatttattct tcatcctcat acaatgagtc tcagttaaga actgactttt
       61 tagacccttt ctttggttta cttggatggg atataactaa ctcaaagaat aaactcacat
      121 ctgaaagaga agtgattgtt gaagaaccac taaaagaaaa agtttcagaa aatgtaaaaa
      181 aaccagatta tacatttaga ttcaattctc aaagaaagtt ttttgttgaa gctaaaaagc
      241 caagtgtcaa aatcaagact gaccctttac ctgctcatca ggtaagacga tatggcttca
      301 cagcaaaatt aagtatttct gttttatcta attttgaata tcttgctata tatgattgtt
      361 cagaaatagt tgatgtacaa caagatgctc tatacgcaag ggtacgatta tatcactata
      421 cagaatatgt gaaatttttt gatgaaatta tgaatctctt aggtagagaa agtgtttatt
      481 caggtcattt tgataatgaa tggcgtgaaa tctctcaaaa aatagaaaac tctaagcaaa
      541 cagtggatga actatttttt gaacaaatta ataagtggcg gcatgttctg ggtagtgaat
      601 tacttaaaat taacccttgt atttcaaaca atgaattaaa tgatgcagtt caagactata
      661 ttaatagcat aatcttttta agagtatgtg aagatcgaag tattgaatgt tatgaatctt
      721 tgctggatgt cacaaagtta gaaaaacata aaaaatttaa aacaaactta gaaaaagctg
      781 acaagaaata taac
//
LOCUS       DANLRK010000297          789 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.298, whole genome shotgun sequence.
ACCESSION   DANLRK010000297 DANLRK010000000
VERSION     DANLRK010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..789
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.298"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            7..678
                     /locus_tag="PXA33_002615"
     CDS             7..678
                     /locus_tag="PXA33_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HDL5121891.1"
                     /translation="MKKSTLAIILGGALLTGTAVAHQAGDVIFRAGAVHVKANSSSDA
                     KSKAATGIDVDLEVKNNTQLGLTATYMLADNVGIELLGATPFSHKINAKAKVLESGAE
                     VDLDRVVILKQLPPSLYAQYYFFELTAKVKPYVGAGLNYTRFYHAKSVSPAVTDLSVK
                     KHSFGPVANLGVDVKLTDNIYFNAAAWYTRIKTTAKFKAAGADHEVKVKLDPVVLFAG
                     FGVKF"
BASE COUNT          257 a          134 c          160 g          238 t
ORIGIN      
        1 atgaaaatga aaaaatcaac tttagcaatt attttaggtg gggcattatt aactggaact
       61 gcggtggcac atcaagcagg tgatgtgata ttccgtgccg gtgcggtaca tgttaaggca
      121 aattcaagtt cagatgcaaa atcaaaagca gcgacaggta ttgatgtaga tttagaagtg
      181 aaaaacaata ctcagttagg cttaactgca acttatatgt tagccgataa tgtgggtatt
      241 gagttattag gtgcaacccc tttctctcat aaaattaatg ccaaagccaa agttttagaa
      301 tcaggtgcag aagtagattt ggatagagtt gttatcttaa aacaattacc accgagcctt
      361 tatgcccaat actatttctt tgagctaact gcaaaagtaa aaccttacgt tggtgcaggt
      421 ttaaattaca ctcgcttcta ccacgctaaa tcagttagcc ctgctgttac tgacttaagt
      481 gtaaaaaaac attctttcgg accggtagca aacctaggtg ttgatgtaaa attaaccgat
      541 aacatctact tcaacgcggc agcttggtac acacgcatta aaacaaccgc aaaatttaaa
      601 gcagcaggtg cagatcacga agtaaaagta aaacttgatc cggttgtctt atttgccggt
      661 ttcggggtga agttctaatt atttgttggg aacgagttgg gctaatctta gtgattagtc
      721 cttttttgtt aaagttgttt gaatatagcc ctttccactt ttaattaaat aattgaagag
      781 ataataaaa
//
LOCUS       DANLRK010000298          786 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.299, whole genome shotgun sequence.
ACCESSION   DANLRK010000298 DANLRK010000000
VERSION     DANLRK010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..786
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.299"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            <1..>786
                     /locus_tag="PXA33_002616"
     CDS             <1..>786
                     /locus_tag="PXA33_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HDL5121892.1"
                     /translation="FNLDKFDARKFSPLSAKDQIFFKNESFETGKSLNLDEVFDRNLS
                     VETPNGFDVILEDKKTGNLSGVNQSNIKALQFFIYQSQQTEEPLQRRFENSEIYGPFV
                     VKSENHTYNQYFIKAVDAQKEWINTILDSPFIMAVLLMFAGIPLLIWLSFKITKPVKE
                     LTISANAVASGNLETNPKLEAEWIYEIGEVGKSFNHMVISLKNLTQQQQRMISDISHE
                     LKTPLARLQLAIAILRRKTCGLAEIDRIEAEVGKLDQLIKDLLT"
BASE COUNT          288 a          118 c          136 g          244 t
ORIGIN      
        1 tttaatttag ataaatttga tgctcgcaaa ttttctcccc tttctgcaaa agaccaaatt
       61 ttctttaaaa atgaaagctt tgaaacaggg aaaagcttaa atttagatga agtttttgat
      121 agaaatttat cagttgaaac acctaatgga tttgatgtaa tcctagaaga taaaaaaact
      181 ggtaatttaa gtggcgtaaa ccaaagtaat attaaagcat tgcagttttt tatttatcaa
      241 tcacaacaga ctgaagagcc gttacaaaga cggtttgaaa atagtgaaat ctatggtcca
      301 tttgtcgtga aatccgaaaa ccatacttat aaccaatatt ttattaaagc ggtagatgct
      361 caaaaagagt ggattaatac gattttagac tcacctttta ttatggcagt attacttatg
      421 tttgccggaa ttcctttatt aatatggtta tcctttaaaa ttaccaaacc ggttaaagaa
      481 ttaactatct cggcaaatgc ggtagcctct ggtaatttgg aaactaatcc taaattagaa
      541 gcagaatgga tctatgaaat tggggaagtt ggaaaaagct ttaatcatat ggtgataagc
      601 ttaaaaaact taactcagca gcaacaaaga atgatttcgg atatatctca tgagttaaaa
      661 acacctttag ccagattaca actcgctatt gctatattac gccgtaaaac ctgtggtctg
      721 gcagaaatcg atcgcattga agcagaagta ggtaaattag accaattgat taaagacttg
      781 ttaaca
//
LOCUS       DANLRK010000299          754 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.300, whole genome shotgun sequence.
ACCESSION   DANLRK010000299 DANLRK010000000
VERSION     DANLRK010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..754
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.300"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            <1..>754
                     /locus_tag="PXA33_002617"
     CDS             <1..>754
                     /locus_tag="PXA33_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121893.1"
                     /translation="QIQVEYFCFDKKQWIKEVHKYSKGGFKRCVNIPFFRFSKFFLNN
                     AQRTAFSYVTHYIKKHSYAYSKADNIQELLVKFFSRKQPVMQNAFNHQKRIVKWLWAE
                     FWDKNIISMTLRIDGYRNANLWHYLRYQSRIEVLYKIANEHPNLLPLLAYVKPEYWKC
                     SDLFSYQNWVRSRDKQPFILYAKCQDKMLCESKGQWRWLKKQSRIVVDQCFLHVNQWQ
                     LLTEIQMPFKIPVCVICFILTNFSMISCELDRS"
BASE COUNT          262 a          103 c          136 g          253 t
ORIGIN      
        1 tcaaattcaa gtcgagtatt tttgttttga taagaaacaa tggataaagg aagttcataa
       61 atattcaaaa ggtggattta agagatgtgt taatatccct ttctttagat ttagtaagtt
      121 tttccttaat aatgcacaac gaacagcctt ttcatatgta actcattaca ttaagaagca
      181 ttcctatgcc tatagtaagg cagacaatat tcaagaatta ttagtaaagt ttttctctag
      241 aaagcaacct gttatgcaaa atgcatttaa tcatcaaaaa aggattgtta agtggttatg
      301 ggctgaattt tgggataaga atattatctc gatgacactt agaattgatg gttatagaaa
      361 tgctaactta tggcattatt tacgttatca atcacggatt gaggtattgt ataaaatagc
      421 aaatgaacac ccgaatctac taccattatt agcctatgtt aaacctgagt actggaaatg
      481 ctcggactta tttagttatc aaaattgggt aagaagccgt gataaacagc cttttatttt
      541 gtatgctaaa tgccaagata agatgttatg tgaatcaaag gggcaatgga gatggttaaa
      601 aaaacagtcg agaatagttg tagatcaatg ttttctacat gttaatcaat ggcagttatt
      661 aacagaaatt caaatgcctt tcaagatccc agtatgtgtc atatgtttca tacttactaa
      721 ttttagcatg atcagctgtg agttagatcg atct
//
LOCUS       DANLRK010000300          723 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.301, whole genome shotgun sequence.
ACCESSION   DANLRK010000300 DANLRK010000000
VERSION     DANLRK010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..723
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.301"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
BASE COUNT          255 a          146 c           89 g          233 t
ORIGIN      
        1 actcatatct aacttactaa gcaacagtac atctataact ttcacaaaaa taacaggcaa
       61 tactataact aatatcaaaa tagaaaaatc caccatactt tctccattaa agtatttcaa
      121 cttactttat acattacttt aattgcatct tcagaaagta aatcacaacg aggtaatact
      181 tcattgatag ccttccaaga aagtaaatct tgctactcta tagcaccttt cattaaaagt
      241 ggttgtttct cagaataact ttcacgaaat tcttgataag aaattggaaa ttgaattttc
      301 attttcatct ccactagcct tgataatctg taccatcacc acaatcgtag tttgagccag
      361 attggttatt attcgttcta cctggaactc caaacgtagg ctttattggt aaagatgatg
      421 gaattaattc ttttacaccg ctaggtaaag aattttcatg tattgtaata ggtaggctcc
      481 tcgctaaatc aagggctaaa ttaacagaag tactggataa accgcctaaa gcccccctct
      541 tgcaaaccct gtagcaaaag caacgccata agatacaaga tctggttgat tagccttatt
      601 ttcagaattc cctccaacta ctaagcttaa ttgtaaaatt gatggttctt tcatttcatt
      661 ctccctttaa tctaattctg atcacaaaaa ccaatattaa aaataattct aaaaataata
      721 cta
//
LOCUS       DANLRK010000301          722 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.302, whole genome shotgun sequence.
ACCESSION   DANLRK010000301 DANLRK010000000
VERSION     DANLRK010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..722
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.302"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(61..693)
                     /locus_tag="PXA33_002618"
     CDS             complement(61..693)
                     /locus_tag="PXA33_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2625 family protein"
                     /protein_id="HDL5121894.1"
                     /translation="MKNLEQLLDKQSAWLILRDWFTQAKNHYEILASYPEDAGSALVG
                     MQLSTRSPLGAMVYETGGVFIDHGWLRILGSGNEKLPRGLFDWNFGKTFKQSGEQPSH
                     LLVADDAIGGYFAINAGGIGSDLGKVYYYHPKAKKWENTQLGYSEFLGWALAGDLNHF
                     YQDLRWENWQTDVEKLQGHQVLDLTSKIAVPIERHYQGEFAPENMGYSVA"
BASE COUNT          204 a          166 c          128 g          224 t
ORIGIN      
        1 tttctttcgt tctaaaaaaa taagctaaac agcctatatt tcagcctctc ttacaaacac
       61 ttaagccacc gaatatccca tattttcagg ggcaaattcg ccttggtagt ggcgttcaat
      121 cggaacggcg attttacttg taagatccag cacttgatgc ccttgtaatt tctctacatc
      181 ggtttgccag ttttcccaac gcaaatcctg ataaaaatgg tttaaatctc cggctaatgc
      241 ccagcctaga aattctgaat aacctaattg ggtgttttcc cattttttag cttttggatg
      301 ataataataa actttgccaa gatctgagcc gattccgccg gcattaatgg caaaatagcc
      361 accgattgca tcatccgcta ccagcaagtg gcttggttgt tcgccagatt gtttaaaagt
      421 tttgccaaag ttccaatcaa ataagcctcg aggcaatttt tcgttgcctg agcctaaaat
      481 gcgtaaccaa ccgtggtcaa taaatacgcc accggtttca tacaccattg cccctagcgg
      541 tgagcgagta gaaagttgca taccaaccaa tgcactgccg gcatcttcag ggtaactcgc
      601 taaaatttcg taatgatttt tggcttgggt aaaccagtct cttaaaatta accaagccga
      661 ttgtttatct aataattgtt ctaaattttt catttgcaaa atttccttag aatttgaccg
      721 ct
//
LOCUS       DANLRK010000302          720 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.303, whole genome shotgun sequence.
ACCESSION   DANLRK010000302 DANLRK010000000
VERSION     DANLRK010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..720
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.303"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            2..673
                     /locus_tag="PXA33_002619"
     CDS             2..673
                     /locus_tag="PXA33_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL5121895.1"
                     /translation="MTLPEIRRKKLTEWFNTRDIPEKEKSYISQLKSGKSSFGERAAR
                     RLENEYGMPSFYLDYDEETESAIVSAMSIQETQSHQYPIHLIDFKAKAGETGFVNTNY
                     PEIIQSIYFSLDGLLEIVGRKSSNGIEMITIPTDSMSPTINKGDVVFIDTTINYYDNE
                     GVYIFVIDDEVYIKRLQKIPGGIYRALSDNKTYEPFDIKRETLDTAIILGKFIRVLPI
                     NPKDL"
BASE COUNT          253 a          119 c          140 g          208 t
ORIGIN      
        1 tatgaccctg ccagagattc gcagaaaaaa acttacagaa tggttcaaca caagagatat
       61 tccagaaaag gaaaaaagct acatatcaca attaaaatca ggaaaatcat cttttggtga
      121 gcgtgcagca agaagattag agaatgaata tggaatgcct agcttttatt tggactatga
      181 tgaagaaaca gaaagtgcga ttgtgtctgc tatgtcaatt caagaaacac agtctcatca
      241 ataccccatt catctaattg acttcaaagc taaggcgggc gaaacaggtt ttgtcaatac
      301 taactatcct gaaattattc aatctatcta tttctcttta gacggcttgc ttgaaattgt
      361 agggcgaaaa tcaagcaatg gcattgagat gatcaccatt ccaactgata gtatgagccc
      421 aacaataaac aaaggggatg ttgtttttat tgatactaca atcaattatt acgataacga
      481 gggtgtttat atttttgtta ttgatgatga agtttatata aaaagactac aaaaaattcc
      541 aggtggtatt tatagagccc tatcggacaa caagacttac gaaccttttg atataaagag
      601 agaaacctta gatactgcta ttatccttgg gaaatttatt cgagtattgc caataaatcc
      661 taaagacttg taggcattag cgtagcggtt gagcatcgga gctatttgta gccagcggtt
//
LOCUS       DANLRK010000303          717 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.304, whole genome shotgun sequence.
ACCESSION   DANLRK010000303 DANLRK010000000
VERSION     DANLRK010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..717
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.304"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            41..>717
                     /locus_tag="PXA33_002620"
     CDS             41..>717
                     /locus_tag="PXA33_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HDL5121896.1"
                     /translation="MPNFSFTHLYKKHTKELPTVTLLSALIGLSQVSYANPLPLGNTT
                     GTNNLAVGEGSFALGTGSQAIGKNAIATGGNISPQEFNVELENFRDLVNQIEELRTQI
                     AEQEKQGSVNQQLQDALKSQIAQYDTILERVREKQAQQAILANSVISMRKELVNRRNQ
                     LTELENNFNTSFLTGKNKQEAYQKFSTVIESLDWDKLSKTNGVDQLATDLKTKIETDF
                     PNINFSN"
BASE COUNT          274 a          121 c          122 g          200 t
ORIGIN      
        1 taagaatgaa aagtaaaagt tttaatgagg aaattttgat atgccaaact ttagtttcac
       61 ccatctgtat aaaaaacaca caaaagagct ccctactgtt accttgctta gcgctctgat
      121 agggcttagc caagtctctt atgccaatcc acttccttta ggcaacacca ctggcaccaa
      181 taaccttgct gtaggtgaag gtagttttgc gttaggcaca ggttctcaag caatcggcaa
      241 gaatgccatt gcaaccggtg gtaatatctc cccccaggaa ttcaatgtgg agttagaaaa
      301 cttccgtgat ttagttaatc aaattgaaga gttacgtact caaattgctg aacaagaaaa
      361 acaaggctct gttaatcaac aattacaaga tgctttaaag agtcagattg ctcaatatga
      421 cactatttta gagcgtgtac gtgaaaaaca agcacaacaa gcaatattag ccaatagtgt
      481 tatatcaatg cggaaagaat tagttaatag aagaaatcag ttaacagaat tggaaaataa
      541 ttttaatact agttttttaa caggaaaaaa taaacaagaa gcatatcaaa aattttctac
      601 agtaatagaa tcattagact gggataaatt atcaaaaact aatggggtag atcaattagc
      661 aacagactta aaaactaaaa ttgaaactga tttccctaat attaattttt caaatga
//
LOCUS       DANLRK010000304          695 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.305, whole genome shotgun sequence.
ACCESSION   DANLRK010000304 DANLRK010000000
VERSION     DANLRK010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.305"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            96..374
                     /locus_tag="PXA33_002621"
     CDS             96..374
                     /locus_tag="PXA33_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bor family protein"
                     /protein_id="HDL5121897.1"
                     /translation="MKKLLAIATVFSLAACSTQTLELSPAKEAQYDRVQHFFVSGLAQ
                     KQEIDAAKICNGADKVGKVETETTFFNGLLGTITYGIYSPRQIRVYCN"
     gene            399..695
                     /locus_tag="PXA33_002622"
     CDS             399..695
                     /locus_tag="PXA33_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bor family protein"
                     /protein_id="HDL5121898.1"
                     /translation="MKKLLAMSVLTALLTTACTTQTAHIGGKVTDNIKPTKSVTQSFF
                     IGGIGQEETLNVVEVCGSKDKVQQVETVLSGGDILLGLVTIGIYTPRTANVYCK"
BASE COUNT          230 a          112 c          136 g          217 t
ORIGIN      
        1 acggatttga accaagttca gcaaataaat gctgttgcta taacttataa aggttggtag
       61 aatataatcc atttaaaaat aatggagact tttcaatgaa aaaattactt gcaattgcaa
      121 cagtattttc tttagcagcc tgttctactc aaacacttga actatctcct gctaaggaag
      181 ctcaatatga tcgtgttcaa cacttctttg tttcagggct tgctcaaaaa caagagatag
      241 atgcagcaaa aatttgtaat ggtgcagata aggtaggtaa ggtagaaact gaaactactt
      301 tctttaatgg cttgttaggt acaattactt atggtatcta ttcacctcgc caaatccgtg
      361 tatattgtaa ctaatcctag taatttaagg agttgaaaat gaaaaaattg cttgctatgt
      421 ctgtattaac agccctgctg actacagcat gtacaacaca aactgcccat attggcggca
      481 aagttactga taatattaaa ccaactaaga gtgtaactca gtcgttcttt ataggtggga
      541 ttgggcaaga agaaacattg aatgttgtag aggtttgtgg ttccaaagat aaagtgcagc
      601 aggttgaaac agtactttca ggtggggaca ttctattagg tcttgtgact atcggtattt
      661 atacacctcg tactgcaaat gtatattgta agtaa
//
LOCUS       DANLRK010000305          677 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.306, whole genome shotgun sequence.
ACCESSION   DANLRK010000305 DANLRK010000000
VERSION     DANLRK010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..677
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.306"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(<1..>677)
                     /locus_tag="PXA33_002623"
     CDS             complement(<1..>677)
                     /locus_tag="PXA33_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121899.1"
                     /translation="EVARKIVIEFLNEVDIGNKVKFYYPDERHELFVELLIVLATLRI
                     YRNRNIFFFEKGFFNFFPEFITILGTGAFNGFLDFPSLVGKVFPEIIDTINEGLDLDI
                     AIENFEVLSQEEMTECMGLYEQALIKKAEEGEIGLLNVDEINSFYEDNSELILLAKQP
                     MVEEIIPKPDSKASSAILKFFDSNSNILIAKLKESGLQLSAVALQNDQNIARVANVVY
                     NLLPGMV"
BASE COUNT          224 a          138 c           86 g          229 t
ORIGIN      
        1 cttaccatgc ccggcaataa gttataaacg acatttgcta ctcgtgcgat attttggtcg
       61 ttttgtaatg ccacagcact taattgcaaa ccactttctt ttaatttagc aattaagata
      121 tttgagtttg aatcaaagaa ttttagaatt gcagaggagg ctttgctatc tggtttaggg
      181 attatctctt caaccatagg ttgtttagca agtaagatta gctcactatt atcttcataa
      241 aagctattta tttcatcaac atttaataaa ccaatttcgc cttcttcagc tttcttaatg
      301 agagcctgct catataaacc catacattca gtcatttctt cctgactcaa cacttcaaag
      361 ttctcaatcg caatatctaa atctaaaccc tcatttatcg tatcaataat ttctggaaaa
      421 acctttccta ccaaactagg gaagtctaaa aatccattaa acgcaccagt cccgagaata
      481 gtgataaatt cgggaaaaaa gttaaaaaac cccttttcaa agaagaatat atttctatta
      541 cgatatattc ttaatgttgc tagaacaatg agcagctcta caaaaagctc atgtctttca
      601 tctgggtaat aaaacttaac tttattacca atatccactt catttagaaa ttctataaca
      661 attttccttg ctacctc
//
LOCUS       DANLRK010000306          661 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.307, whole genome shotgun sequence.
ACCESSION   DANLRK010000306 DANLRK010000000
VERSION     DANLRK010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..661
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.307"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            165..>661
                     /locus_tag="PXA33_002624"
     CDS             165..>661
                     /locus_tag="PXA33_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121900.1"
                     /translation="MASALGAFLLLLVASSYADFYYTQTKQKEQLYLQAEAHQLLNYF
                     QQHFQHIGYQGSHRTDSNFDLFQQAGKSLNIVHKNCIIGFYDLNQDGCLGKRKTKTAA
                     CKLGTVNNTREVLKEIVGFKVENNEIYTFSSQLDNCVKEQCQTLLENCDGNWSKFTEA
                     NNFKT"
BASE COUNT          246 a           96 c          125 g          194 t
ORIGIN      
        1 tggggtaagt ggtcaattag aatcaaaatg tattaagctc agtttaaata aagaaaatga
       61 aattttgcaa tttgaacaat taggacgggt ttatgtggca gcaaaaaata aacgtagcac
      121 ctgcaaagat tagtttatct gcttttagcc taattgaagt attaatggct agtgcattag
      181 gggcgttttt attattgctt gttgcttcaa gttatgccga tttctattac acacaaacca
      241 aacaaaaaga acagctctac ttgcaagcag aagctcacca gttactaaac tacttccaac
      301 agcattttca acacattggc tatcaaggct ctcacagaac agacagtaat tttgatttat
      361 ttcagcaagc agggaaaagc ctgaatattg tgcataaaaa ttgcattatt ggattttacg
      421 atttaaacca agatggctgt ttgggaaaac gtaagacaaa aacggccgct tgtaagttag
      481 gaacagtgaa taataccaga gaggtgttaa aagaaattgt tggctttaaa gtagaaaata
      541 atgaaattta tactttttca tcgcaattag ataattgtgt gaaagaacaa tgccaaacat
      601 tattggaaaa ttgcgatgga aattggagta aatttaccga agcaaataat ttcaaaacta
      661 a
//
LOCUS       DANLRK010000307          645 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.308, whole genome shotgun sequence.
ACCESSION   DANLRK010000307 DANLRK010000000
VERSION     DANLRK010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..645
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.308"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(19..303)
                     /locus_tag="PXA33_002625"
     CDS             complement(19..303)
                     /locus_tag="PXA33_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010128390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121901.1"
                     /translation="MANITLSHITPKHFFETVRLLKMQEHRIYIGVDSIEVRFKQKRF
                     ESKKETDKRLEQLEHFMLFLQEIINSDIPIDLDEFIYTYHRSVRRGFYRR"
     gene            complement(321..482)
                     /locus_tag="PXA33_002626"
     CDS             complement(321..482)
                     /locus_tag="PXA33_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007525341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HDL5121902.1"
                     /translation="MEKIENSSNLVLKLSLENQMLILESLEHPKEPNLVMLEALKKYS
                     LYVAKTSNK"
BASE COUNT          200 a          112 c          103 g          230 t
ORIGIN      
        1 cactcctctt tcacatcact aacgtcgata aaaacctctc cttactgacc tgtgataggt
       61 gtatataaac tcatctaaat ctataggaat atctgaatta atgatttctt gtagaaagag
      121 cataaaatgc tctaactgtt ctagtctctt atctgtttcc ttttttgact caaaacgctt
      181 ttgcttaaaa cgaacttcta tcgaatctac accgatgtaa attcgatgtt cctgcatttt
      241 gagtagtcgg acagtttcaa aaaaatgttt aggggtgatg tgagaaaggg ttatatttgc
      301 catagtttag cttgaaaacg ctatttatta cttgttttag caacatacaa agaatacttt
      361 tttaaagcct ctagcattac taaattaggc tctttaggat gttctaagct ctcaagaatt
      421 agcatttgat tttcaagaga aagctttaga acaagatttg aactattctc tattttttcc
      481 attttaatta tctcattaga tgactttcgc ataacaacag gttatgtgta aattctcgtg
      541 cagtgggaca agcccgattg tactgcactc gaattgtaac ataacctgta ggacattatg
      601 cgaaaattaa taatataagt ttttcatcaa tcggcggtat tattg
//
LOCUS       DANLRK010000308          644 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.309, whole genome shotgun sequence.
ACCESSION   DANLRK010000308 DANLRK010000000
VERSION     DANLRK010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..644
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.309"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            <1..>644
                     /locus_tag="PXA33_002627"
     CDS             <1..>644
                     /locus_tag="PXA33_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDL5121903.1"
                     /translation="EIEQTRAAYDRLKRSGVASQNELRRASEATKQRVRELNAELGKT
                     SFGDKAKGFGRGLMSAGVGIAAGAAMARQPAKNQMEFDRQLAMASNTAFSDRDVKGRI
                     EGKKELLAAVQEAVKVGGGTKEDALLTLDTLIASGTMKAETAMSLLPTLQKGAVATGA
                     SSEDMAKIAISSMQQFGIKEEDIGRALDMAVAAGQAGNFELADMARWLPQQMAAG"
BASE COUNT          184 a          129 c          190 g          141 t
ORIGIN      
        1 gaaattgaac aaactcgtgc agcctatgac cgcttaaaac gctcaggtgt tgcctctcaa
       61 aatgagctac gccgtgcctc agaagccact aagcaacggg tgcgagagct gaatgcagaa
      121 ttgggtaaga caagctttgg ggataaggct aaaggatttg ggcgtggttt gatgagtgcc
      181 ggtgtcggta ttgcagcagg tgctgcaatg gctcgtcagc cagccaagaa ccaaatggag
      241 tttgaccgac agttagcgat ggcatcaaac actgcctttt ccgaccgtga tgttaaaggg
      301 cgtattgagg gcaagaaaga gttattagca gcggtgcaag aggcggttaa ggttgggggt
      361 ggaacaaaag aagatgctct tttaactctt gatacgctaa ttgcctcagg aacgatgaag
      421 gctgaaactg caatgagcct attaccaacc ttgcagaaag gtgctgtagc aacaggtgca
      481 agctccgagg atatggcaaa aattgcgatc tcctcaatgc aacaatttgg gattaaagaa
      541 gaggatattg ggcgggcatt ggatatggca gttgcagccg ggcaagcagg taactttgag
      601 cttgcagata tggctcgctg gctaccacaa caaatggccg cagg
//
LOCUS       DANLRK010000309          639 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.310, whole genome shotgun sequence.
ACCESSION   DANLRK010000309 DANLRK010000000
VERSION     DANLRK010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..639
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.310"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            <1..>639
                     /locus_tag="PXA33_002628"
     CDS             <1..>639
                     /locus_tag="PXA33_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDL5121904.1"
                     /translation="LEGFKMKVVHNGLEGLNELESNQYDLVLLDVMMPVLSGVKTLKR
                     IRQKYSLPVLMLSARDDQIDRILGLELGADDYVPKPFNNRELVARMKAILRRTTSRPA
                     STDHSEQNYTENISKSLQFAGVELHSGRQQAYYQGCDLDLTGTEFALLQMLMRNSGEI
                     LSRELLSLKILGKNLTPYDRAIDMHISNLRKKLPEREDGLPWFKTLRGRGYL"
BASE COUNT          221 a           93 c          129 g          196 t
ORIGIN      
        1 cattagaagg atttaaaatg aaagttgtgc ataatggttt agaaggctta aatgagttag
       61 agtctaatca atatgattta gtattactag atgtgatgat gccggtatta agtggcgtga
      121 aaacattaaa acgtatacga caaaaatact cattacccgt tttaatgtta agtgcaagag
      181 atgatcaaat tgatagaatc ttaggtttag aattaggggc tgacgattat gtaccgaaac
      241 cttttaataa tcgtgaacta gtcgctagaa tgaaagcgat acttcgaaga acaacctctc
      301 gcccagcttc aacagaccat tctgaacaga attatactga aaacatctct aaatcattac
      361 aatttgccgg agttgaatta cattcaggac gtcagcaggc ttattatcaa ggatgtgatc
      421 ttgatttaac ggggactgaa tttgcattat tacaaatgtt aatgcgaaac tcaggggaga
      481 ttttgtccag agaattattg agtttaaaaa ttttagggaa aaatttaacg ccttatgatc
      541 gtgcaattga tatgcatatt tctaatcttc gtaaaaaatt acccgaacgg gaagatggat
      601 taccttggtt taaaacctta agaggtcggg gttacttat
//
LOCUS       DANLRK010000310          628 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.311, whole genome shotgun sequence.
ACCESSION   DANLRK010000310 DANLRK010000000
VERSION     DANLRK010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..628
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.311"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            <1..120
                     /locus_tag="PXA33_002629"
     CDS             <1..120
                     /locus_tag="PXA33_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDL5121905.1"
                     /translation="VPKLNQRNLNSILIAIPPISEQHRIVEKIEKLFSEIEKF"
     gene            complement(166..>628)
                     /locus_tag="PXA33_002630"
     CDS             complement(166..>628)
                     /locus_tag="PXA33_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL5121906.1"
                     /translation="FVIRFMAATGSRVSELIQIKCEHIKIGYLDLYSKGGKLRRIYIP
                     KSLQKECSEWLRLNNKHNGFIFLNKYGDRITTRGIASQLKVFARRYKLDPKIVYPHSF
                     RHRFAKSFLERFNDIAFLADLMGHESIETTRIYLRKTSTEQQEIVNKIIDW"
BASE COUNT          218 a          110 c           84 g          216 t
ORIGIN      
        1 gtacccaaat taaatcaaag aaacctaaac tctattttaa ttgctattcc acctatttct
       61 gaacaacata gaatcgttga aaaaattgaa aaattatttt cggaaataga gaaattttag
      121 aaaaacttat atgtccaagg gcgtgtgaaa cacgcccctt aaaaattacc aatcaataat
      181 tttattcaca atttcctgct gttctgtgct tgtttttcgt aaatagatcc ttgtggtttc
      241 tatgctttcg tgccccatta aatcagcaag aaaagcaata tcattaaatc gttctaaaaa
      301 actttttgca aaacgatggc gaaatgaatg tggataaaca attttaggat ctaatttata
      361 gcgtctagca aatactttta attggctggc aatgccacga gtagtaattc tatcaccata
      421 tttatttaag aaaataaatc cattatgctt attatttaat ctcaaccatt cagaacactc
      481 tttttgcaga gattttggga tgtaaattcg tcttaatttt ccacctttag aatagaggtc
      541 taaatagcct attttaatat gttcacattt tatctggatt aattcactta ctctcgaacc
      601 tgttgctgcc ataaaacgaa tcacaaaa
//
LOCUS       DANLRK010000311          628 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.312, whole genome shotgun sequence.
ACCESSION   DANLRK010000311 DANLRK010000000
VERSION     DANLRK010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..628
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.312"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            91..486
                     /locus_tag="PXA33_002631"
     CDS             91..486
                     /locus_tag="PXA33_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HDL5121907.1"
                     /translation="MKKLMIFATTAMIVSNLAHAAGEQSDAREAHESTVKTSTVKYSC
                     QNGKKLSVKYGFNKQGIPTYAEAKLSGKKRFMPINLYTTDATGTNFGDENNFSLYGDP
                     MEFTNHRKASVNIQSPASEILYKGCTPQK"
BASE COUNT          243 a          114 c          105 g          166 t
ORIGIN      
        1 tttttgaaaa aatatggaac tttatataaa actcactttc taaacaatac gttatctgaa
       61 ctaagataac gcttatttaa aggaaaatat atgaaaaaat tgatgatatt cgcaacaact
      121 gccatgattg taagcaattt agcccatgca gcaggtgaac aaagtgatgc aagagaagcc
      181 cacgaaagca ccgtaaaaac ctcaactgtt aagtatagct gccaaaacgg caaaaaatta
      241 agcgtgaaat acggcttcaa taaacaaggc attccaacct atgctgaggc aaaattaagc
      301 ggtaaaaaac gttttatgcc gatcaaccta tacacgacag atgcaaccgg cactaacttt
      361 ggtgacgaaa acaatttcag cctttacggt gatccaatgg aatttaccaa ccaccgtaaa
      421 gcatcagtaa atatccaaag ccccgcaagt gaaattttat ataaaggttg cacacctcaa
      481 aaatagataa aaagtaaaat gagtccgctc tgataagtgg ctttttaata gctgtttatt
      541 gcagatagga aagcattttg tttttatcta ctatccagaa acaagcggtt aaatttgttt
      601 aattttttac aaaaaatggg ccaaaatt
//
LOCUS       DANLRK010000312          601 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.313, whole genome shotgun sequence.
ACCESSION   DANLRK010000312 DANLRK010000000
VERSION     DANLRK010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..601
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.313"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            <1..300
                     /locus_tag="PXA33_002632"
     CDS             <1..300
                     /locus_tag="PXA33_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121908.1"
                     /translation="IINKAKNQNSQENNLTEINSNIQILAEKHQLTIESLQWNLEQGK
                     SIELSIIGNSRSLFDFIHSVKQIPYLKYNAISLIKSRQDRKVEMNTTLVILANKE"
     gene            303..>601
                     /locus_tag="PXA33_002633"
     CDS             303..>601
                     /locus_tag="PXA33_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121909.1"
                     /translation="MRNWFLFITLFISFSVKANDPFFGEKQETQASGNIAESNVEFAK
                     NTENITACKVPDNLSAKNIVTDFDKMTLIGLVKINEVMRALFIDEKQQLLALKEN"
BASE COUNT          246 a           92 c           89 g          174 t
ORIGIN      
        1 attatcaata aagctaaaaa tcagaacagc caagagaata atttaaccga aataaacagc
       61 aacatacaaa tattagctga aaaacaccaa ctaaccattg aaagtttaca atggaattta
      121 gaacaaggta aaagtattga attgagtatt atcggcaata gccgatcttt atttgatttt
      181 atccattcag ttaagcaaat accttatttg aaatacaatg cgattagcct catcaaatct
      241 cggcaagaca gaaaagttga aatgaatact accttagtca tattagctaa taaggaataa
      301 caatgcgtaa ttggttttta tttattactt tatttatcag tttttctgta aaagcaaatg
      361 atcctttctt tggagaaaaa caggaaactc aagcctcagg caacatcgct gaaagcaatg
      421 tggaatttgc aaaaaatact gaaaacatca ccgcttgtaa agtacctgat aacctttctg
      481 caaaaaatat agtaactgat tttgacaaaa tgacactgat aggattagtt aaaatcaatg
      541 aagtaatgcg tgcattattt attgatgaaa aacaacaatt acttgctcta aaagaaaatg
      601 a
//
LOCUS       DANLRK010000313          590 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.314, whole genome shotgun sequence.
ACCESSION   DANLRK010000313 DANLRK010000000
VERSION     DANLRK010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..590
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.314"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            <1..320
                     /locus_tag="PXA33_002634"
     CDS             <1..320
                     /locus_tag="PXA33_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121910.1"
                     /translation="YLEEYNDLGTIRYKFQEENLYGFLRDGATLVANGIVNEPSVDCF
                     SQEIAQFTGCHIFSSLYIAFNTQRSFKSHWDSRDIFAVQMQGKKRWIIHTPTFKKTIV
                     YAS"
     gene            310..>590
                     /locus_tag="PXA33_002635"
     CDS             310..>590
                     /locus_tag="PXA33_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin-like domain-containing protein"
                     /protein_id="HDL5121911.1"
                     /translation="MHRSKDMPEYAPNLDDVYMDIVLEAGDVLYLPRGWWHDPIPVGE
                     ETVHLAVGIFPAYTHNYLTWVSQNMVEKEIARASLSHYESDKELIAQLA"
BASE COUNT          183 a           84 c          117 g          206 t
ORIGIN      
        1 attatttaga agaatataat gatctaggta ctattcgtta taaatttcaa gaagaaaatt
       61 tatatggttt tttaagagat ggggcaacat tagtcgcaaa tggtattgta aatgaaccaa
      121 gtgttgattg cttctctcaa gagatagctc aatttacagg ctgtcatatt ttttcaagtt
      181 tatatattgc gtttaatact caaagatctt ttaaaagcca ttgggatagt cgtgatattt
      241 ttgcagttca aatgcagggt aaaaagcgtt ggattattca cactccaaca tttaaaaaaa
      301 ccattgttta tgcatcgtag taaagatatg cctgaatatg ctcctaatct tgatgatgta
      361 tatatggata ttgtattaga agctggagat gttctttatt tacctcgtgg ctggtggcac
      421 gatcctattc ctgttggaga agaaactgtg catttagctg taggcatttt tcctgcttat
      481 actcataact atctgacttg ggtatctcaa aatatggttg agaaagaaat tgcccgagct
      541 tctttatcgc attatgagtc agataaagag ttgatagcac agttggcaga
//
LOCUS       DANLRK010000314          588 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.315, whole genome shotgun sequence.
ACCESSION   DANLRK010000314 DANLRK010000000
VERSION     DANLRK010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.315"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            <1..>588
                     /locus_tag="PXA33_002636"
     CDS             <1..>588
                     /locus_tag="PXA33_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDL5121912.1"
                     /translation="LNTVLNTLQCDYVLFSSEDNYQVIINLKKADYPKNEANFITMTL
                     NKKFGDAKFSGANHYLRCASFFNKKSTNNNEKSVLVDFTNTKTEEDNKCYFDNLLSSY
                     KNNNVKLEPLDIKIIDELGDDKAVIAQKEIQAEIALCKRIFKQLDWSAVDFRIVKRLY
                     RKGFSENEIAVALVRFTDFEDRHCDSHDYLTRTITK"
BASE COUNT          232 a           89 c           91 g          176 t
ORIGIN      
        1 ctaaacacag ttttaaacac actacaatgc gattatgtgc tgttttcttc tgaagacaac
       61 taccaagtca tcatcaatct aaaaaaagct gattacccca aaaatgaagc aaatttcatt
      121 acaatgactt taaacaagaa atttggtgat gcaaaatttt ccggtgcaaa ccattactta
      181 agatgtgcca gcttcttcaa caagaaaagc acaaacaaca atgaaaaatc agttttagtt
      241 gattttacaa acacaaagac agaagaagac aataaatgtt attttgataa cttattatct
      301 agttataaaa ataacaatgt aaaacttgag cctttagaca tcaaaattat tgatgagctt
      361 ggagatgata aagcagttat tgcacaaaaa gaaattcaag cagaaattgc tttgtgtaaa
      421 cgtatattca aacaattaga ttggtctgct gttgatttta gaattgttaa gcgtctgtac
      481 aggaaaggat tttcagaaaa tgaaattgct gttgcacttg tcagatttac tgattttgaa
      541 gacagacatt gtgattcaca tgattatttg acaagaacaa tcacaaaa
//
LOCUS       DANLRK010000315          568 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.316, whole genome shotgun sequence.
ACCESSION   DANLRK010000315 DANLRK010000000
VERSION     DANLRK010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..568
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.316"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(<1..389)
                     /locus_tag="PXA33_002637"
     CDS             complement(<1..389)
                     /locus_tag="PXA33_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121913.1"
                     /translation="MAVSAYIGIPGSGKSYEVVKSVILPAVASNRRVVSNIYGLNKEK
                     IHQYLLRKNKKLMIEELGEVIYVTNEQCLESDFLPSMDEQDSFCKAGDLIIIDEVWRI
                     WGNDKDIPKNHRSFIAEHRHFADPITGV"
     gene            complement(399..>568)
                     /locus_tag="PXA33_002638"
     CDS             complement(399..>568)
                     /locus_tag="PXA33_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2523 domain-containing protein"
                     /protein_id="HDL5121914.1"
                     /translation="EMLVSRKLIPDLNALFGQLPESMWYFLNIFQIPMAISLMISAMI
                     SRFVRRMPFIG"
BASE COUNT          195 a          120 c           74 g          179 t
ORIGIN      
        1 acacctgtta taggatctgc aaaatgtcga tgttcagcaa taaatgaacg atgatttttt
       61 ggaatatcct tgtcgtttcc ccaaatacgc caaacttcat caataattat caagtcccca
      121 gctttgcaaa atgaatcttg ttcgtccatt gatggtaaaa aatccgattc caagcattgt
      181 tcattcgtta catatatgac ttcaccaagc tcttcaatca ttaatttttt atttttacgc
      241 agtaaatatt gatgaatttt ctctttattt aatccataaa tgttggaaac aactcttcga
      301 ttcgaagcaa cagcaggtaa aattactgac ttaaccactt catacgattt gccgcttcca
      361 ggaatcccta tatacgctga aacagccata gcactcctct atccaataaa tggcatacgt
      421 ctaacaaaac gtgaaatcat tgctgaaatc attaaagaaa tagccattgg tatctgaaaa
      481 atatttaaaa aataccacat actttcaggc aattgcccaa ataacgcatt taaatcaggt
      541 atcaattttc tagacacaag catctcta
//
LOCUS       DANLRK010000316          559 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.317, whole genome shotgun sequence.
ACCESSION   DANLRK010000316 DANLRK010000000
VERSION     DANLRK010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..559
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.317"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(<1..>559)
                     /locus_tag="PXA33_002639"
     CDS             complement(<1..>559)
                     /locus_tag="PXA33_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121915.1"
                     /translation="FLSACFNESYDFRGKEFEEPVQAILVESDKIYFIGSTIDAIFDK
                     TLIDNTKASDVIYFLNKLLQSPYGRKISNHLITLAIYDENIQIQYSPQFIAKNLTMSD
                     IQALQKLGFQFSHSKEYLWKSYAGLGGKITQFKNRKEILEKYKFKQPIIAKAKSYQKS
                     IEFSFDDTISVIAAPITIPASIILFV"
BASE COUNT          178 a           89 c           88 g          204 t
ORIGIN      
        1 tacaaataaa ataatagatg cgggaatcgt aattggtgct gcaatgaccg aaatggtgtc
       61 atcaaagcta aattctatgc ttttctgata acttttcgct ttagcgatta taggttgctt
      121 aaatttatac ttttctagta tttccttgcg atttttaaac tgtgtgattt taccaccaag
      181 acctgcatag cttttccata gatattcttt tgaatggcta aattgaaaac ctaacttttg
      241 tagagcttga atatcgctca tggtgagatt ttttgcaata aattgcggac tgtactgaat
      301 ttgaatattt tcatcataaa tggctaaagt aattaggtga ttactgatct tccttccata
      361 cggactttga agaagtttat tcaaaaaata aataacatct gaagcttttg tattatctat
      421 cagcgtttta tcaaatatcg catcaattgt ggaaccaatg aaataaattt tatctgattc
      481 aaccaaaatt gcttgtacag gttcttcaaa ttcttttcct ctaaaatcat aactttcgtt
      541 aaagcatgcg gagagaaac
//
LOCUS       DANLRK010000317          552 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.318, whole genome shotgun sequence.
ACCESSION   DANLRK010000317 DANLRK010000000
VERSION     DANLRK010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..552
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.318"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(<1..>552)
                     /locus_tag="PXA33_002640"
     CDS             complement(<1..>552)
                     /locus_tag="PXA33_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlwI family type II restriction endonuclease"
                     /protein_id="HDL5121916.1"
                     /translation="KDLGITIKSPEQAATYVNDERYRRFNTLIDKKFNDSVLIELLNC
                     FEKRDDKRIEELVTDEAAIPTIFEYILGIIWYKVSERQGNILDFMKLSLEANLLPKTH
                     AAGGYADIIYEYEACTSYPKHSLLLEATLADGNNQRRMEMEPVSRHLGDYRIRFNNPF
                     DYSLFVSTYLDKNVISDFRYRKII"
BASE COUNT          169 a          108 c           72 g          203 t
ORIGIN      
        1 aattatcttt ctatatctaa aatctgagat tacattttta tctaagtatg tgctaacaaa
       61 taggctgtaa tcaaacggat tattaaatct aatcctataa tcgcctaaat gtcttgaaac
      121 aggctccatt tccattcttc tttgattatt tccatctgca agggttgctt caagcaataa
      181 tgaatgtttc ggataagagg tgcacgcttc atattcatat ataatgtctg cgtaacctcc
      241 agctgcgtgg gtcttcggca ataaattggc ttctaatgat aacttcatga aatccaaaat
      301 atttccttgt ctttcgctca ctttgtacca tataattccc aaaatatatt caaaaatagt
      361 aggtattgca gcctcatctg taacaagttc ttcaatcctt ttatcatcac gtttctcaaa
      421 acaatttagt aactctataa gcacagaatc gttaaatttc ttatcgatca aagtattaaa
      481 tcttctgtat cgttcatcat ttacataagt tgcagcttgt tccggactct taattgtaat
      541 tcctaaatct tt
//
LOCUS       DANLRK010000318          550 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.319, whole genome shotgun sequence.
ACCESSION   DANLRK010000318 DANLRK010000000
VERSION     DANLRK010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.319"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            <1..>550
                     /locus_tag="PXA33_002641"
     CDS             <1..>550
                     /locus_tag="PXA33_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HDL5121917.1"
                     /translation="IDTVNASVGSNSSKITQVSNAVASVDGKLSATHTIKTETIAGGR
                     KAIAGIAFGATADNRNAESSVIVMADKFEVVKNAQDGTPVRLFGVVENKVAINGNLIA
                     TGSISGDHINANSVRTAVLTAGAIKTEHLAAGQISADKLAIGLGGNLLVNPIFATPDL
                     SIAPFGWNYWRGEVGDRLNIDTR"
BASE COUNT          163 a          111 c          142 g          134 t
ORIGIN      
        1 attgatacgg tcaatgcctc agtcggatca aactcatcta aaattaccca agtcagcaat
       61 gcggtagcaa gtgtggacgg caaattatct gctacccata ccatcaaaac cgaaaccatt
      121 gccggtggac gcaaagcgat tgccggtatt gcatttggtg caacggcaga taaccgtaat
      181 gcggaatctt ctgtgattgt gatggcagat aaatttgagg tcgtgaaaaa cgcccaagac
      241 ggcacgccag taagactatt tggcgtggta gaaaataaag tagcgattaa tgggaattta
      301 atcgcaacag gctcgatttc aggagaccac atcaatgcaa attccgtcag aactgctgta
      361 ttgactgcag gagcgattaa aacggagcat ttggcagcag ggcaaatatc ggcggataag
      421 ttagcgattg ggctaggtgg gaatttgctg gttaatccta tttttgcaac acccgatttg
      481 tcaatagctc cgtttggctg gaactactgg cgaggagaag ttggtgatcg gctaaatatt
      541 gacacccgta
//
LOCUS       DANLRK010000319          550 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.320, whole genome shotgun sequence.
ACCESSION   DANLRK010000319 DANLRK010000000
VERSION     DANLRK010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.320"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            <1..>550
                     /locus_tag="PXA33_002642"
     CDS             <1..>550
                     /locus_tag="PXA33_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HDL5121918.1"
                     /translation="IDAVTASVGSNLAKITQVSNAVAGVDGKLSATHTIKTETIAGGR
                     KAIAGIALGAAADNRNAESSVIVMADKFEVVKNAQDGQPVRLFGVAQNKVAINGNLIA
                     TGTIKGSHIDADSVRAGILTAGAIRTEHLAAGQISADKLAIGLGGNLLVNPIFATPDL
                     SIAPFGWNYWRGEVGDRLNIDTR"
BASE COUNT          156 a          111 c          145 g          138 t
ORIGIN      
        1 attgatgccg ttactgcttc tgttggctca aacttggcaa aaattactca agtgagtaat
       61 gctgtagccg gtgtggacgg caaattatct gctacccata ccatcaaaac cgaaaccatt
      121 gccggtggac gtaaagcgat tgccggtatc gcacttggtg ccgcggcaga caatcgcaat
      181 gcagaatcct ctgtcattgt aatggcagac aaatttgagg tagtaaaaaa tgctcaagac
      241 ggccaaccgg tacgattgtt tggtgtggct caaaataaag ttgcgattaa tgggaattta
      301 attgcaacag gtacaattaa aggtagccac attgatgcag actcagtaag agccggaata
      361 ttgacggcgg gagctatccg tacggaacac ctggcagcag ggcaaatatc ggcggataag
      421 ttagcgattg ggctaggtgg gaatttgctg gttaatccta tttttgcaac acccgatttg
      481 tcaatagctc cgtttggctg gaactactgg cgaggagaag ttggtgatcg gctaaatatt
      541 gacacccgta
//
LOCUS       DANLRK010000320          548 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.321, whole genome shotgun sequence.
ACCESSION   DANLRK010000320 DANLRK010000000
VERSION     DANLRK010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.321"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            33..233
                     /locus_tag="PXA33_002643"
     CDS             33..233
                     /locus_tag="PXA33_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL5121919.1"
                     /translation="MNLTSYLSDRPRGFKADFAKKLGISKSYLRQVETGYSPMPAYLA
                     KKIEEVTNGEVAKSELRPDLWD"
     gene            254..385
                     /locus_tag="PXA33_002644"
                     /pseudo
     CDS             254..385
                     /locus_tag="PXA33_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250081.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
BASE COUNT          168 a          100 c          117 g          163 t
ORIGIN      
        1 ctcaatctaa tttttactta acgggagtgt taatgaactt aactagttat ttgtcagatc
       61 gccctcgtgg tttcaaagct gactttgcaa aaaagcttgg tatttccaaa tcttatttac
      121 gacaagttga aacagggtat tcaccaatgc cagcctatct tgctaagaaa attgaagaag
      181 tcacgaatgg cgaagtagcc aaatctgagc ttcgccccga tctgtgggat taatttaccc
      241 aaaggaatgt gcaatgccac gcaatgaatt aacaaaaaat gcaagagcaa tcggactgag
      301 tgaattacaa tttagccgta catttgcaga taatgtggaa atggtggcgg tgattattga
      361 ttacttaagt attgagcttg cggatattcg tttcgcatta cgattagata atattcaagg
      421 taaagctaat ccatttttat ttgctcgtag gctaatgctt gaggaggcta gtgataatgt
      481 tacagatatc actcagccta gcccttggca aaatgccggt gtaaccgcta ttcacggtgg
      541 ctcaatag
//
LOCUS       DANLRK010000321          547 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.322, whole genome shotgun sequence.
ACCESSION   DANLRK010000321 DANLRK010000000
VERSION     DANLRK010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..547
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.322"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            <1..>547
                     /locus_tag="PXA33_002645"
     CDS             <1..>547
                     /locus_tag="PXA33_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121920.1"
                     /translation="HCTLLNACSKALGFSGGFAGFQEDYLSNLTQFMEKHGLLYLVDL
                     TKQKYPVNDMCMTRSLALKKQDISERIFFSGKEIPKRIFTGFNFRYDMHLANGFRIGN
                     SLKTPYSDIGICEDINEALKNPSIKIDCRDGTIRSMADVVIGRYLDKVLFYYFNLIGD
                     QLVQPRLDKYEIQCYYPQGYQG"
BASE COUNT          192 a           75 c          103 g          177 t
ORIGIN      
        1 cactgtactt tgttaaatgc ttgctcaaaa gctttaggat tttctggagg ctttgcaggg
       61 tttcaagaag attatttatc taatttgacg caattcatgg aaaaacatgg gctcttatat
      121 cttgtagatt taacaaaaca aaaatatcca gtaaatgata tgtgcatgac aaggagctta
      181 gctctgaaaa agcaagatat atctgaaaga attttcttta gcggaaagga aatccctaag
      241 agaatattta ctggatttaa tttccgatat gatatgcatt tagctaatgg ttttcgtatc
      301 ggaaattcct taaaaacccc atattctgac ataggtatat gtgaagacat aaatgaagca
      361 ttaaaaaatc catcaataaa aattgattgc cgtgatggca caatcagaag tatggcagat
      421 gtagtcatag gtagatatct tgataaagta ttgttttatt attttaactt gataggtgat
      481 cagttagtcc agcctagatt ggataaatat gaaatacaat gttattaccc tcaaggttat
      541 caagggg
//
LOCUS       DANLRK010000322          543 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.323, whole genome shotgun sequence.
ACCESSION   DANLRK010000322 DANLRK010000000
VERSION     DANLRK010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..543
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.323"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            <1..>543
                     /locus_tag="PXA33_002646"
     CDS             <1..>543
                     /locus_tag="PXA33_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDL5121921.1"
                     /translation="ANGAKMPRGDKNAMKGFIFNIPSIEYQKKCIANYFAFDQKIQLN
                     TQTNQTLEAIAQAIFKSWFVDFDPVRAKAAALSEGKSEHEANLAAMSVICGKDTSELN
                     DTEYKALWQIAEAFPSEFGDEGLPIGWKFNQADNLFDVGIGKTPPRKESEWFSDNAND
                     TEWISIKDMGNQGLFITESS"
BASE COUNT          193 a          106 c          117 g          127 t
ORIGIN      
        1 agcaaatgga gcaaaaatgc cacgagggga taaaaatgca atgaaaggtt ttattttcaa
       61 tatcccaagt attgaatatc aaaaaaaatg tattgctaat tattttgcct ttgaccaaaa
      121 aatccaactc aacacccaaa ccaaccaaac cctagaagcc attgcacagg caatcttcaa
      181 aagctggttt gtggattttg accctgtgcg cgccaaagcc gccgccctaa gcgaaggcaa
      241 aagcgaacac gaagccaacc ttgcggcaat gtcggtgatt tgtggtaaag acaccagcga
      301 gttaaacgac accgaataca aagcgctttg gcaaatcgcc gaagccttcc caagtgagtt
      361 tggagatgaa gggttaccta ttggctggaa attcaatcaa gcagacaatt tatttgatgt
      421 tggtattggg aaaaccccac caagaaaaga gagtgaatgg ttttctgata atgcaaatga
      481 tacagaatgg atttctatta aagatatggg aaatcaggga ttatttatca cagaaagtag
      541 cga
//
LOCUS       DANLRK010000323          543 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.324, whole genome shotgun sequence.
ACCESSION   DANLRK010000323 DANLRK010000000
VERSION     DANLRK010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..543
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.324"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(103..228)
                     /gene="ykgO"
                     /locus_tag="PXA33_002647"
     CDS             complement(103..228)
                     /gene="ykgO"
                     /locus_tag="PXA33_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HDL5121922.1"
                     /translation="MKILNSLKTAKSRHPDCQIVRRKGKLYVICKTNPRFKARQR"
     gene            complement(237..509)
                     /locus_tag="PXA33_002648"
     CDS             complement(237..509)
                     /locus_tag="PXA33_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005709555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HDL5121923.1"
                     /translation="MKKGIHPENYREVLFYDASVQQGWVIRSCAATNKTMVWEDGKEY
                     PLYTLDTSSASHPVYTGKRREVNTEGRASQFKDRFKGFASTLSNQK"
BASE COUNT          157 a           92 c          109 g          185 t
ORIGIN      
        1 ggaataagaa ataataaaat cgttgatgtg gttaaaatag gtaatattga gaagatgaaa
       61 taaaaaacac cttaataaat tagcttatta aggtgctggt ttttaacgct gacgagcttt
      121 aaatctcggg ttggttttgc aaatcacata aagtttgcct ttgcgtctga caatttggca
      181 atccgggtgg cgtgattttg cggttttaag tgagtttaag attttcattt tatcctctat
      241 ttttgatttg aaagcgttga agcaaagcct ttaaatctat ctttaaattg acttgcacga
      301 ccttctgtat tcacttcacg gcgtttacct gtgtaaacag ggtgagacgc cgatgaggta
      361 tctaaggtgt aaagtgggta ttctttgcca tcttcccata ccattgtttt gttggttgcc
      421 gcacaagaac ggataaccca gccttgttgc actgaggcat catagaacaa cacttcacga
      481 taattttcag gatgaatccc ttttttcata ttttctccag aatttagata aggtcattaa
      541 cca
//
LOCUS       DANLRK010000324          532 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.325, whole genome shotgun sequence.
ACCESSION   DANLRK010000324 DANLRK010000000
VERSION     DANLRK010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..532
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.325"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            <1..296
                     /locus_tag="PXA33_002649"
     CDS             <1..296
                     /locus_tag="PXA33_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121924.1"
                     /translation="DTRYNKNGQGAVSERNIIDAKNRFKASHPYAKIISCVRGSETYV
                     TRPAKANATTKSVKEKQSQPQESSSLSSMLLGAAVTAGVGFVANKWFSKKDNT"
     gene            324..>532
                     /locus_tag="PXA33_002650"
     CDS             324..>532
                     /locus_tag="PXA33_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121925.1"
                     /translation="MAGLLPPYFKKKNSDHYKDAVKQFGKEQVDEVIAQCDELESWVK
                     EMPTKQLIMYVGCFHTFNKLSQSKA"
BASE COUNT          197 a           83 c          100 g          152 t
ORIGIN      
        1 cagataccag atataacaag aatgggcaag gtgctgtttc tgaaagaaat attatagatg
       61 ctaaaaatcg ttttaaagcg agccatccat acgcaaaaat tatttcttgt gttagagggt
      121 ctgagacata tgtaacacgt ccagcaaaag ctaatgctac aacaaaatct gtcaaggaaa
      181 agcaatctca accacaagaa agttcatcgc tcagttcaat gttacttggt gcggctgtaa
      241 ctgctggggt tggttttgta gccaataaat ggtttagcaa aaaagataac acttaattta
      301 tctctaacca atgtggagta aaaatggctg gattattacc accttatttc aaaaagaaaa
      361 atagtgatca ctataaagat gctgtaaaac aatttgggaa agaacaagtt gatgaggtta
      421 tagcacagtg tgatgaactt gaatcttggg taaaagaaat gccaacaaaa caacttatta
      481 tgtatgttgg ttgtttccat acttttaaca aattatctca atctaaagct ta
//
LOCUS       DANLRK010000325          507 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.326, whole genome shotgun sequence.
ACCESSION   DANLRK010000325 DANLRK010000000
VERSION     DANLRK010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.326"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            <1..>507
                     /locus_tag="PXA33_002651"
     CDS             <1..>507
                     /locus_tag="PXA33_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HDL5121926.1"
                     /translation="NLVTAIPNFLEKVNNDFAISARNRDLYSHYLPYQGIDDWENSNF
                     NFRTLNGVYYDDNLTSTELLRKFMIQRDYNTLYALSSARTGNADSLAKNYHLVVAKMS
                     YENLKKELSKKPDSTFNKLKKLYETNGEFLNEYILDNFRGNPLELLTVPALKESLENQ
                     ESFPSRLYQ"
BASE COUNT          184 a           87 c           82 g          154 t
ORIGIN      
        1 aacttagtaa cagccattcc taacttcctg gagaaagtta ataatgattt tgctataagt
       61 gctagaaata gagacttata tagtcactac cttccttatc agggtattga tgattgggag
      121 aattctaatt ttaactttcg aacactaaat ggagtttatt atgatgataa tctaacttca
      181 acagaactcc tgcgaaaatt tatgattcaa agagattata acactcttta tgctctaagt
      241 tctgctcgca caggaaatgc tgatagtctg gctaaaaatt accatcttgt agttgcgaaa
      301 atgtcctatg aaaacctgaa aaaagaatta tccaagaaac ccgactctac atttaataaa
      361 ctgaaaaagc tgtatgaaac caatggtgaa ttcttaaatg aatatatttt agacaatttc
      421 cgaggaaatc cacttgaatt attaacagta cctgcgctta aagaaagttt agaaaaccaa
      481 gagtcattcc ctagcaggct gtatcag
//
LOCUS       DANLRK010000326          497 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.327, whole genome shotgun sequence.
ACCESSION   DANLRK010000326 DANLRK010000000
VERSION     DANLRK010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.327"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            32..457
                     /locus_tag="PXA33_002652"
     CDS             32..457
                     /locus_tag="PXA33_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDL5121927.1"
                     /translation="MYKHILVAVDLSEESLVLVRKGAALAEKCGAKLSLIHVDVNFSD
                     LYTGLIDINMSSVQYSVVEETNTALAELASKVNYPVSECLNGTGDFSQVLEEAVEKHN
                     VDLLITGHHQDFWSKFMSSTRRVMNNITVDMLVVPLADE"
BASE COUNT          159 a           81 c          105 g          152 t
ORIGIN      
        1 gtaaacaact ctcacaagca aggagcatat tatgtataaa catatcttag ttgcagtgga
       61 tctttctgaa gaaagtcttg ttttagtgcg taagggggct gcgttagcag aaaaatgcgg
      121 agctaaactt tccctcattc acgttgatgt taatttttcg gatctctata caggattaat
      181 tgatattaat atgtcttcgg tacaatacag tgttgttgaa gaaacaaata cggcactggc
      241 tgaacttgcc tcaaaagtaa attatcctgt atctgaatgt ttaaacggaa cgggagattt
      301 tagtcaagtt ttggaagagg ccgtagaaaa acacaatgtc gatttactca tcactggcca
      361 tcatcaggac ttctggagta aatttatgtc ttcaactcgt cgagtcatga ataatatcac
      421 tgttgatatg ctggttgtgc cattagcaga tgaatagcaa gaaagtgaat gaaaaataac
      481 cgcttgagac agtttat
//
LOCUS       DANLRK010000327          483 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.328, whole genome shotgun sequence.
ACCESSION   DANLRK010000327 DANLRK010000000
VERSION     DANLRK010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.328"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(187..>483)
                     /locus_tag="PXA33_002653"
     CDS             complement(187..>483)
                     /locus_tag="PXA33_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HDL5121928.1"
                     /translation="MTEAEKVLWQALRAKRFLAIKFKRQQVIGNYIVDFVCFSAKLVI
                     ELDGSQHLDQQNYDNLRTHFLNGQGFRVLRFWNNQIHSDLEAVLDTIFYEITRG"
BASE COUNT          166 a           90 c           72 g          155 t
ORIGIN      
        1 tcccacaagg ggagagggtt attctttcaa aaattcaaaa caactaaaaa attagcattc
       61 tccttttgta ggagaaaatg ttgttctttc aaaattctaa aataaccgca aaattaactc
      121 tcgccccttg tgggagagag acagataaat cattcgttca gaatgattta tcagagagag
      181 ggggaattac cctctcgtaa tttcataaaa aattgtatct aaaacagcct ctagatcgct
      241 atgaatttga ttattccaaa atcttaaaac cctgaacccc tgaccgttta aaaaatgagt
      301 tcgtagatta tcatagtttt gttgatctaa atgttgtgaa ccatctaatt caataactaa
      361 tttcgctgaa aagcaaacaa aatcgacaat atagttacct ataacttgtt gccgtttaaa
      421 ttttatcgct aaaaatcgtt ttgctcttaa tgcttgccaa agaaccttct ctgcttctgt
      481 cat
//
LOCUS       DANLRK010000328          470 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.329, whole genome shotgun sequence.
ACCESSION   DANLRK010000328 DANLRK010000000
VERSION     DANLRK010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..470
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.329"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
BASE COUNT          168 a           87 c           77 g          138 t
ORIGIN      
        1 attttctaac ttccatcttc gtttttctaa aatcgaaaag taatactggt catcgtctaa
       61 aatctcaata tatgtacttc caaagagaac ttttgtataa ggcgtatcat gatagatcgt
      121 gacatcagcc ttcaatttgt actcgccttc catgcattcc ccaaaatggt attgactaaa
      181 aacaatggtt atgatataat gacatggggc ttgaaaagca agctgattgc tcaataggta
      241 atcagaccac tttgaaagct cggtcctttt caaaagaaaa ggtcttacta attactcaca
      301 aaatctggag aacaatatga aaaacactac agcgcaacaa aagccaatga aagttacagc
      361 taaacatatt agagaatcta ctactcgttg ttaagagctt agctttgata taaaaagacc
      421 aactttttat atcggcgtag taggtctaat ttgagcctac gaaatgaaag
//
LOCUS       DANLRK010000329          451 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.330, whole genome shotgun sequence.
ACCESSION   DANLRK010000329 DANLRK010000000
VERSION     DANLRK010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.330"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            complement(<1..>451)
                     /locus_tag="PXA33_002654"
     CDS             complement(<1..>451)
                     /locus_tag="PXA33_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015483985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL5121929.1"
                     /translation="LSHLTLFDFWAVGDGVTNDESAFVNAMRYSQFFIENGTFRINNA
                     VNSVRDNVKILGNKTGKLVLGAGIQQAGAEVFNINHSNYFISGFCIETPNKAIGIRFK
                     SLDDAGVKNLHIDNVVFNGTFYGVRAGESIQADTNYPTDNVIVQNCQS"
BASE COUNT          147 a           95 c           74 g          135 t
ORIGIN      
        1 gattggcagt tttgtacaat cacattatca gttgggtaat tagtatccgc ctgaatactt
       61 tcacctgccc tgaccccgta gaaggtgcca ttaaacacaa cattgtcaat atggagattt
      121 ttaacaccag catcatccaa agacttaaat cgaataccaa ttgctttgtt cggtgtttca
      181 atacaaaaac ccgagataaa ataattgctg tgattaatat taaaaacttc ggcccctgct
      241 tgctggatac ctgcacctaa tactaatttg ccggtcttat tgcccaaaat tttcacatta
      301 tcgcgaacag aattaacggc attgttgata cgaaatgtac cgttttcgat aaaaaattga
      361 gaatatctca tagcatttac aaatgctgat tcatcatttg ttaccccgtc accgacggcc
      421 caaaaatcaa ataatgtaag atgtgaaagg t
//
LOCUS       DANLRK010000330          424 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.331, whole genome shotgun sequence.
ACCESSION   DANLRK010000330 DANLRK010000000
VERSION     DANLRK010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.331"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
BASE COUNT          143 a           80 c           60 g          141 t
ORIGIN      
        1 ttagttatat cctgaaaaat caaattataa agaaacctta tctatcaata cttgaagcca
       61 ttaagttcaa gtctcgacca ggcaccattt gactaaattt agttcaacag agctaaacga
      121 aaaaacctta gaaagcctgt aaaatcaacg tttacaggct tttttgttgt tttagctcaa
      181 tggcactaaa catttcatat cgcaaatttt aagtagccta gcaaattttc cgccaaatat
      241 gaccgcttgt aatgccgtta ggttattgcc tggcggcctt tttgttacag ctcttatatt
      301 ttttacacca aagttcagaa aaagtcactt ttatcactaa cattaagatg aaaccttgat
      361 acaaagcttg ggcttcggtg atatttaaaa aatatcactt ttttcaataa ttgtgacaaa
      421 tctc
//
LOCUS       DANLRK010000331          420 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.332, whole genome shotgun sequence.
ACCESSION   DANLRK010000331 DANLRK010000000
VERSION     DANLRK010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.332"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            142..>420
                     /locus_tag="PXA33_002655"
     CDS             142..>420
                     /locus_tag="PXA33_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HDL5121930.1"
                     /translation="MKNRIEHPKIFISYAWGSQEHDEKVIALATNLKGDGIDVIFDKW
                     QLKEGNDTFKFMEKSVLDESITNVLILIDPIYAKKANERAGGVGTETQI"
BASE COUNT          160 a           45 c           96 g          119 t
ORIGIN      
        1 aataccatga aatgccatta gagactgtag attggcatga tgggatgata aaagaggcta
       61 ctggagagta taagtcatag tgattataag atagtgagaa agaatactta ttttgaaagg
      121 aatacatggg aattgataca gatgaagaat agaattgaac atcctaagat ttttatttca
      181 tatgcttggg gatcacagga acatgatgag aaggttattg ctttagctac aaatcttaaa
      241 ggagatggta tagatgtcat ttttgataaa tggcagctaa aagaaggcaa tgataccttt
      301 aaattcatgg aaaaaagtgt tttagatgag agtattacca atgtactgat tcttatagat
      361 cctatttatg caaagaaagc aaatgaaaga gccggtggcg taggtacaga aacacagatt
//
LOCUS       DANLRK010000332          417 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.333, whole genome shotgun sequence.
ACCESSION   DANLRK010000332 DANLRK010000000
VERSION     DANLRK010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.333"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            38..>417
                     /locus_tag="PXA33_002656"
     CDS             38..>417
                     /locus_tag="PXA33_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL5121931.1"
                     /translation="MKNIETKWLEDFLTLEECRHFSQAAEKRNLSQSAFSRRILALEE
                     VVGVKLFDRTSIPLQLTEQGKLFHSQTRNLLQQLQNNLDELLGHNCNLPNIKFAAAHS
                     LSLSIMPKLIKKLSQTNENFIYSV"
BASE COUNT          154 a           67 c           64 g          132 t
ORIGIN      
        1 aatgaaatca taatgcgaaa tttgcattgg ttaggatatg aaaaacatag aaacaaaatg
       61 gttagaagat ttcttaacgc tagaagaatg tcgccatttt tctcaggcag cagaaaaaag
      121 aaatttgtca caatctgcat ttagcagacg tattttagct ttagaagaag ttgtgggagt
      181 gaagttattt gatcgtacct ctattccatt acaactcaca gaacaaggta agctatttca
      241 ttcacaaacg cgaaacttat tacaacaatt acagaataat ctagatgaat tattaggaca
      301 taattgtaac ttacctaata ttaaatttgc ggcggctcat tccctctctc tttctattat
      361 gcccaaatta attaagaaat tatcacaaac aaatgaaaat tttatttatt ccgttga
//
LOCUS       DANLRK010000333          411 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.denovo.334, whole genome shotgun sequence.
ACCESSION   DANLRK010000333 DANLRK010000000
VERSION     DANLRK010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.denovo.334"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            141..>411
                     /locus_tag="PXA33_002657"
     CDS             141..>411
                     /locus_tag="PXA33_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5121932.1"
                     /translation="MELGKTTETDVKEMYNLEPEGVNKYSKGNQYFIDPTQLDLEDLK
                     STLIIFDEKGVLVFVGSEFNSISSIEDSKNRYKKFDYLYKILASKY"
BASE COUNT          154 a           55 c           71 g          131 t
ORIGIN      
        1 aatagcatcc ccgcaagaat gaggagatgg ctatttttgt tatttatagc ttataggaaa
       61 ctcattatga aaaagatgtt aaagctacat tatttgcaac tattagtact tctgtattag
      121 ctgaaccaac cgtttttaaa atggaactag gtaaaacaac agaaacagat gtaaaagaaa
      181 tgtataacct tgaaccggag ggtgttaata aatacagcaa gggtaatcag tattttattg
      241 atccaacgca attagattta gaagatctta aatcaacgct tattatcttt gatgaaaaag
      301 gggtattagt ttttgttggt tccgagttta attctatatc ttctatcgag gattcaaaaa
      361 accggtataa aaaattcgat tatttgtaca aaatactagc aagcaaatat a
//
LOCUS       DANLRK010000334         4075 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995767-rid16722043.guided.191, whole genome shotgun sequence.
ACCESSION   DANLRK010000334 DANLRK010000000
VERSION     DANLRK010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995767
            Sequence Read Archive: SRR18050877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574734.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/13/2023 00:17:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,661
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 34 of 60
            Pseudo Genes (incomplete)         :: 19 of 60
            Pseudo Genes (internal stop)      :: 9 of 60
            Pseudo Genes (multiple problems)  :: 2 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4075
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995767-rid16722043.guided.191"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: ND"
                     /collection_date="2020-06-02"
                     /collected_by="North Dakota State University Veterinary
                     Diagnostic Laboratory, Fargo, ND"
     gene            <1..1314
                     /locus_tag="PXA33_002658"
     CDS             <1..1314
                     /locus_tag="PXA33_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HDL5121933.1"
                     /translation="QPQAVKFEQNVVKNSQKPTAYQPAYSVNQNVEQQINQAILSQDW
                     ATLEPLLSRYPQTENFDPLLWHYAQAALAYAKKDHQTAVFHYRQLLAKPPSLVYPRFD
                     LALILAEDQQFRAAEQEFKAIQAHLPPELAKIARNTQVQIAENEAWQPDFYLQYTQTD
                     NVNNASSSPIVNVNGRIFRKNADSLPQSAKGVRYGLGLSKLHNVKGNHFVGLTLNYSG
                     IYYWDNRDYREQSVSVSPNYSYRTVAYRLTFSPFIEQNWLGSSRYNVQFGATLNGLRY
                     LNSQWLVSGSFTHLQKRYFEELTASRYNSYQHQLSGGVQWQAVKNGRFFANLDGVREI
                     AREKAQSSNKWLARVGAVFQGERWATQASIGFGKRYFADKHYLFGYKRLDTEYQANLA
                     VWNRTWHWQGIVPKLNFRYQKIDSNIADFYSRQNKEVFFTLEKLF"
     gene            1460..1933
                     /gene="gpt"
                     /locus_tag="PXA33_002659"
     CDS             1460..1933
                     /gene="gpt"
                     /locus_tag="PXA33_002659"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDL5121934.1"
                     /translation="MSEKYTNEKYVVTWDMFHMHARKLAENLLPATQWKGIIAVSRGG
                     LFPAAVLARELGIRHVETVCIASYDHDSQGELNVLHAADTDGEGFIVVDDLVDTGNTA
                     KEIRRMYPKARFVTVFAKPHGAPLVDDYVIDIPQETWIEQPWDLGITFVQPLARR"
     gene            2046..2669
                     /gene="gmk"
                     /locus_tag="PXA33_002660"
     CDS             2046..2669
                     /gene="gmk"
                     /locus_tag="PXA33_002660"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HDL5121935.1"
                     /translation="MSLGNLYIISAPSGAGKSSLINALLADLPRSEVQLSISHTTRQP
                     RPAEQDGVHYYFTSHDEFKQLIEQGYFLEWAEVFGNYYGTSLPMIERSLEQGIDVFLD
                     IDWQGARQIREKVPNVKTVFILPPSKTELEKRLIGRGQDSAETIAKRMAEAVSEMNHY
                     NEFDYVIINDDFQQALAELKAILIAERLSLGSQQIRHQHLITDLLNN"
     gene            2759..>4075
                     /locus_tag="PXA33_002661"
     CDS             2759..>4075
                     /locus_tag="PXA33_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HDL5121936.1"
                     /translation="MKLSKLAFSVALALTVTACTTGNMSNDGTLQQAQQTYQSYDEIT
                     KQYQINEQWWQGYTDAELNRVVDLAIKNNLDLAKSGIAVNKALYNANLVGANLVPTFS
                     GSGSVSAAKGLGSASSNNVSTGVSTTAAQAAFNLSYTVDLWRRLADTASAAEWTHKAT
                     IEDLKATRLSIINAVVTTYYQLAYYRDAIRITEESIRHYEQISRILTNKMNAGAIDSL
                     AVDQAQQAVLNARNSVIDLKTNQKIAEQTLRNLLNVQPNTPLNIRYPNIMSVKLQGVD
                     LNVPVSAIANRPDVVGSLQRLQSAFKTLTATEKSWFPTLTIGGSITGSATNIGNVADN
                     PVGNGLIKLDLPFLDWNRVKNNVKISETDYVTARLNYEQKITSALNEIDRYYYSYTQS
                     RSSLKILQDTNAKNKRISSYYKNRYEQGVSEFREWITALNTELNSQL"
BASE COUNT         1194 a          802 c          928 g         1151 t
ORIGIN      
        1 cagccgcaag cggttaaatt tgagcaaaat gttgtaaaaa attcacaaaa accgaccgct
       61 tatcagcctg cgtattcagt caatcagaat gttgaacagc aaattaacca agcgattctc
      121 agccaagatt gggcaacgct tgaaccgctt ttaagccgtt atccgcaaac tgaaaatttt
      181 gacccgttgt tgtggcacta tgcgcaagcc gcacttgcct atgcgaaaaa agatcatcaa
      241 accgcagttt ttcactatcg ccaactgtta gctaagcccc cgagtttggt ttaccctcgt
      301 tttgatttgg cgttgatttt ggcagaagat cagcaatttc gtgcggcaga gcaggaattt
      361 aaggctattc aagcccattt accgccggaa cttgctaaaa ttgcccgcaa tactcaagtc
      421 caaattgctg aaaatgaggc gtggcagccc gatttttatc tgcaatatac ccaaacagat
      481 aacgtgaata atgcctcatc ttcgccgatt gtgaatgtaa atgggcggat tttccgcaaa
      541 aatgcagaca gtttgccaca atcagcaaaa ggcgtgcgtt atggcttagg gttgagtaaa
      601 ttgcacaacg tgaaaggcaa tcattttgtc ggtttaacgc tcaattatag cgggatttac
      661 tattgggata accgagatta ccgtgagcaa tccgtatccg tttcgccgaa ttatagctac
      721 cgcaccgtcg cttatcggct gactttttcg ccttttattg agcaaaattg gctggggagt
      781 agccgttata atgttcaatt tggggcaaca ctcaacggtt tgcgttacct taattcgcaa
      841 tggttggtgt cggggagttt tacccatttg caaaaacggt attttgagga gctaaccgca
      901 agccgttata acagctatca gcaccaactc agtggcggtg tgcagtggca agcggtcaaa
      961 aatgggcggt tttttgcaaa tttagacggc gtgcgtgaaa ttgcacggga aaaagcacaa
     1021 agctcgaata agtggctggc tcgagtgggg gcggtttttc aaggcgaacg ctgggcaacg
     1081 caggcaagta tcggctttgg caagcgatat tttgccgaca agcactattt gtttggctat
     1141 aagcggttag acacggaata tcaagccaat ttagcggttt ggaaccgcac ttggcattgg
     1201 cagggcattg tgccgaagct gaatttccgt tatcaaaaaa ttgacagcaa tattgctgat
     1261 ttttactctc gccaaaacaa ggaagtcttt ttcactttag aaaagctatt ttagtgttgg
     1321 ttttttgtgc taagcatacg attgcgtagc tgccggcaca aaaaaataaa tgaaatgatg
     1381 gcgaaaaaag cattttcggg ttatcctagt gcaatttttt atatcggggt agcccccaat
     1441 tttatattag gttgtttcta tgagtgaaaa atacacaaat gaaaaatatg tcgtgacgtg
     1501 ggatatgttc catatgcacg ctcgcaagtt ggcagaaaat ttattacctg ccacacagtg
     1561 gaaagggatt attgcggtga gccgtggcgg cttgtttccg gcagccgttt tagcacgtga
     1621 gttgggtatt cgccacgtag aaacagtgtg cattgccagc tacgatcacg atagccaagg
     1681 tgagttaaat gtgttacacg cagccgatac cgatggcgaa ggctttattg tggtagatga
     1741 tttagtggat accggcaaca cggcaaaaga aattcgcaga atgtacccga aagctcgttt
     1801 tgtaaccgtg tttgcgaaac cgcacggtgc accgttggtt gatgattatg tgattgatat
     1861 tccacaagaa acgtggattg agcagccttg ggatttaggc attaccttcg ttcagccttt
     1921 ggcacgtaga taattttgtg taatggacaa tgaaagttgt ccattatttt ttatttattc
     1981 ctatgaaatg taggggcgaa tcatcattcg ccctattccc aaaatcaaat aggtatatac
     2041 aaataatgag tttaggcaat ctttatatta tttctgcccc aagtggagca ggaaaatctt
     2101 ccttaattaa cgccttgttg gcggatttac cccgttcgga agtacagctt tcgatttcac
     2161 acactacccg tcagcctcgt ccggcagaac aggacggcgt gcattattat ttcacttcgc
     2221 acgatgagtt taagcagctg attgagcaag ggtatttttt agaatgggcg gaggtgtttg
     2281 gcaattatta cggcacatca ttgccgatga ttgaacgtag cctagagcag ggcattgatg
     2341 tctttttgga tattgactgg caaggggcta gacaaattcg ggaaaaagtg ccgaatgtga
     2401 aaaccgtttt tattttgcca ccgtcaaaaa cagaattgga aaaacgttta atcgggcgtg
     2461 ggcaagattc cgctgaaacc attgccaaac gtatggcaga ggcggtgtcg gaaatgaacc
     2521 attacaatga gttcgattat gtgattatta atgatgattt ccaacaggca ttggcagagc
     2581 taaaagcgat tttaattgcc gagcgtttga gcttaggttc gcaacaaatt cgtcatcagc
     2641 atttgattac tgatttgtta aataattaaa tgaacagttg ttcatttaac tggaaaggca
     2701 atctgttttt gggtagaata cgcccatttt cccctcttta ttttcaaaag gaaatgtaat
     2761 gaaactttca aaattagcat tttctgtggc gttggctcta accgtaacgg cttgtaccac
     2821 agggaatatg agcaatgacg ggactttgca acaagcacag caaacctatc aaagctatga
     2881 tgaaattacc aaacaatatc aaattaacga gcaatggtgg caaggctata cggatgcgga
     2941 attaaaccgt gtggttgatt tagccatcaa aaataacctt gatttagcca aaagcggaat
     3001 tgcagtcaat aaagccttgt ataacgctaa tttagtaggg gcaaatttag tgccaacctt
     3061 tagtggttca ggttctgttt ctgcggctaa aggtttaggt tcggcttctt caaataatgt
     3121 ttcgaccggt gtttcaacaa ccgcagctca agctgcattt aatttaagtt atactgtgga
     3181 tttatggcga cgtttagccg ataccgccag tgcggcagaa tggacgcata aagccaccat
     3241 tgaagactta aaagctaccc gtttatctat tattaatgcg gttgtgacta cttactacca
     3301 gttagcttac tatcgtgatg cgattcgtat taccgaagag agcattcgcc attatgagca
     3361 aatcagccgt attttaacta acaaaatgaa tgccggtgcg attgatagtt tagcggttga
     3421 tcaggctcaa caagcggtct taaacgccag aaatagtgta attgatctga aaaccaacca
     3481 aaaaattgcc gagcaaacat tacgcaattt gttaaatgtt cagccaaata ctccgcttaa
     3541 cattcgttac ccgaatatta tgagcgtgaa attgcaaggg gtagatttaa atgtgccagt
     3601 gtcagccatt gctaatcgcc ctgatgtagt aggctcgtta caacgcttac aaagtgcatt
     3661 taaaacctta accgcaaccg agaaaagctg gttcccaacc ttaaccatcg gtggttctat
     3721 taccggtagt gcaaccaata tcggcaatgt ggcagataac cctgtgggta acggcttaat
     3781 taaattggat ttaccgttct tagattggaa tcgggttaaa aataacgtaa aaatttctga
     3841 aacggattat gtgactgccc gtttaaacta tgagcaaaaa attacctcag ccttaaatga
     3901 aattgaccgt tattattaca gttatacgca gtcgagaagc tcgttaaaaa tccttcaaga
     3961 taccaatgcg aaaaataaac gtatcagttc atactataaa aaccgttatg aacaaggggt
     4021 gtcggagttc cgtgagtgga ttaccgcact gaataccgag cttaattctc agttg
//
